LOCUS       DATALY010000001         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_777521,
            whole genome shotgun sequence.
ACCESSION   DATALY010000001 DATALY010000000
VERSION     DATALY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777521"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..89)
                     /locus_tag="VHR42_00005"
     tRNA            complement(16..89)
                     /locus_tag="VHR42_00005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Gly,seq:ccc)
     gene            complement(524..688)
                     /locus_tag="VHR42_00010"
     CDS             complement(524..688)
                     /locus_tag="VHR42_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025604.1"
                     /translation="MRNGKDEIVVQNEEILQQLEFLNRNLNTVATNLTLIFQKIESIE
                     SKLPEKEKQG"
     gene            complement(923..1432)
                     /locus_tag="VHR42_00015"
     CDS             complement(923..1432)
                     /locus_tag="VHR42_00015"
                     /inference="COORDINATES: protein motif:HMM:NF025628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HEX3025605.1"
                     /translation="MKTEMLNRKKWIIFSVVALVAALVILTGSYVLFQNSRENAEPLK
                     PAQITVRIINEMNRTDLAEVSGSQLSKHYNIPDGVITESSLYMSRSSDSASELACFYL
                     ADKSKFAQLQTAVTGHLNSKAAGFKSLNPAQYNALKNASVTQKGRYVLVSVGSNPAAD
                     AKLFNEILK"
     gene            complement(1848..>3349)
                     /gene="thrS"
                     /locus_tag="VHR42_00020"
     CDS             complement(1848..>3349)
                     /gene="thrS"
                     /locus_tag="VHR42_00020"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX3025606.1"
                     /translation="EKANQTYKVELIEEHSGKGEKITFYKQGDYTDLCAGPHLMSTGC
                     VKAFKLTAATAAYWRGDSKNKMLQRVYGISFPKTSELEEYLAKIEDAKKRDHNVLGRQ
                     LGYFTTSDLIGQGLPILLPNGARVIQLLQRFVEDEEQRRGWLLTKTPYMAKSDLYKLS
                     GHWDHYLDGMFVLGDPEKDEEVFALRPMTCPFQYQAYLNKQRSYRDLPLRYNETSTLF
                     RNEASGEMHGLIRVRQFTISEGHLMCTPAQLENEFKGCLELAIYMLKTLGLYEDVSYR
                     FSQWDPNNRAKYIGTAEQWDEAQGIMKKILDHLQIPYFVGIDEAAFYGPKLDIQIKNV
                     FGKEDTLITIQIDQMLAEKFGMEYVDVDGKKKNPYIIHRTSIGCYERTLALLIEKYAG
                     ALPLWLMPEQIRILPISERLHPDAEKVLEKLQNAGFRATCDTRSEKIGYKIREAQLQK
                     IPYMLVIGDKEVENGTVAVRSRKDGDLGTMELDAFIAKAAGEVATKAKD"
BASE COUNT          796 a          824 c          751 g          978 t
ORIGIN      
        1 cgtgcctgtc tcttatggag cggaagacgg gaatcgaacc cgcgtcctta gcttgggaag
       61 ctaatgttct accattgaac cactcccgcg ttacattttt gtattttaac acaaactctt
      121 ttcagattca agaggaaatt cttattttat gcgcactccg gaaccgtatc gccttacttt
      181 taaagcaaaa accgcatcag gcagctgttt tgggctgctt gatacagttt cgtttagcaa
      241 atttaaaaag ttcatcctca acagacttga aacacaatct gacctcaatc cgatcttatt
      301 tttattgagg atattgtttg ctttctcttc tgaccaatct atcgaatggc cgatagaggt
      361 atgccgcctt ctatactgaa attattccgg ttcccaggca aacgaaatcc acccggtttc
      421 atttttatcg aaggaaagga acggcagatc gtatctgttt tacattctcg gcttttatat
      481 ttcctaatca ggcaccatta cgggccggat gaagcattat gtattatcct tgtttttctt
      541 tttccggcag cttactttca atgctctcaa ttttttgaaa aatcaacgtc aggtttgttg
      601 cgaccgtgtt taaatttcta ttgagaaatt ccagctgctg caaaatttct tcattctgca
      661 caacaatttc gtcttttccg ttacgcatat tcacccctcc taccactgaa taaaattatt
      721 atagcgaaaa atgcataaat tacaattact caaaggaaaa aataaaataa gttttgtacc
      781 gaatataaaa atacgctctt atgcatccgc cagtgacaca taagagcgtt ctacacccgc
      841 ttgccaatgc tgcaggtgct atggttttat tataatccgg tcaatttaaa aattctgtcg
      901 gttcatagac atactcccaa ttttatttta atatctcatt gaacagtttg gcgtctgccg
      961 cagggttgct gcctaccgaa accagaacat atctcccttt ctgcgtaaca gacgcatttt
     1021 tcagggcgtt gtactgcgcg gggttcaggc ttttgaaacc ggcggctttg gagttgagat
     1081 gaccggttac cgcagtctgc agctgcgcaa atttggattt atccgcaagg taaaaacagg
     1141 caagttcgga agcgctgtcc gatgatcggc tcatgtagag tgaactctca gtaatgacac
     1201 cgtccggaat attgtaatgc ttggaaagct gactaccact tacttcggcc agatccgtgc
     1261 ggttcatttc gttgataata cgcaccgtaa tctgggcagg cttaagcggt tccgcgtttt
     1321 cccggctgtt ctggaacaaa acgtagcttc cggttaagat caccagtgcg gcaaccagcg
     1381 caacaacgga aaaaataatc cactttttcc ggttcagcat ttccgttttc aagcctgcgc
     1441 ctccttctta atccgataaa ccattttatt ttactatacc atcagagaat agttattcta
     1501 tataggaata atataataat gtatataatt ttttctttcc taatcactat tttgtgcaaa
     1561 caacagtata aaaatgagtc tatttttcta atttacataa tctttccatt gtgtgcatca
     1621 aatcattaac attttccaca ttgttttcaa ccgtataaag ccttacgcgt ctgatttttt
     1681 acggcttttc aacttttccg gttcccgatt tataacagtc aggtggaaaa tgtttagttt
     1741 acataacaga tcggaaggac cgcaggaggg cggaggtttc cgtacgataa aagccggccg
     1801 gatagaatat ccatccggcc ggctctggct tcggtcaaat atgaagatca gtcttttgct
     1861 tttgtggcta cttcgccggc agctttcgcg atgaatgcgt caagctccat ggtgcccagg
     1921 tcgccgtctt tgcggctgcg caccgccacg gtaccgttct ctacttcctt atcgccgatt
     1981 acgagcatat acggaatctt ctgaagctgt gcttcacgga ttttgtagcc gattttctcg
     2041 ctgcgggtgt cgcaggttgc acggaatccg gcattctgaa gcttctccag aaccttttcg
     2101 gcatccggat gcaggcgctc gctgatcggc agtatgcgga tttgctccgg catcagccac
     2161 agcggcaggg cacccgcgta tttttcaatc agcagggcca gtgtgcgctc gtagcatccg
     2221 atggaggtac ggtggataat gtagggattc ttctttttgc cgtccacatc cacatactcc
     2281 ataccgaatt tttcggcaag catctggtcg atctgaatcg taatcagcgt gtcttccttg
     2341 ccgaatacgt tcttgatctg aatatcgagc ttcgggccgt agaaggccgc ctcgtctatg
     2401 cccacaaaat aaggaatctg cagatgatcc aggatttttt tcataatccc ctgggcctcg
     2461 tcccactgtt ccgcggttcc gatatacttt gcgcggttgt tcggatccca ctgggagaag
     2521 cggtaggaca catcctcata caggccgagc gttttcagca tgtagattgc cagttcaagg
     2581 cagcccttga attcattttc cagctgtgcc ggggtgcaca tcagatggcc ttccgaaatc
     2641 gtaaactggc gcacgcgaat caggccgtgc atctcgccgg aagcctcatt gcggaacagc
     2701 gtggaggttt cgttatagcg cagcggcaga tcgcggtagg agcgctgctt gttcagataa
     2761 gcctgatact ggaacgggca ggtcatcggg cgcagtgcga aaacttcttc atccttttcc
     2821 gggtctccca aaacaaacat gccgtcaagg taatggtccc agtggccgga aagcttgtaa
     2881 aggtcgctct ttgccatata gggcgtcttt gtaagcagcc agccgcgtct ctgttcctcg
     2941 tcctcaacaa agcgctgcag aagctgaatc acgcgggcgc cgttcggaag aagaatcgga
     3001 aggccctgtc cgatcaggtc actggtggta aagtagccga gctgtctgcc cagcacgttg
     3061 tggtcgcgct ttttcgcgtc ttcaatcttt gccagatatt cctcaagctc gcttgtcttt
     3121 gggaacgata tgccgtagac gcgctgcagc attttgttct tggaatcgcc gcgccagtag
     3181 gcggcggttg cggcggtcag cttgaacgcc ttcacgcagc ccgtgctcat aaggtgaggg
     3241 ccggcgcaga ggtcggtata atcgccctgt ttataaaacg taattttttc gcccttgccg
     3301 gaatgctctt cgatcagttc aaccttatac gtctggtttg ccttctcca
//
LOCUS       DATALY010000002         8371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_346323,
            whole genome shotgun sequence.
ACCESSION   DATALY010000002 DATALY010000000
VERSION     DATALY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346323"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..595)
                     /locus_tag="VHR42_00025"
     CDS             complement(26..595)
                     /locus_tag="VHR42_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3025607.1"
                     /translation="MKHRIVIADDEPITRMDIYEILTEAGYEVVGQATDGFQTVELCR
                     ALTPDLAIVDIKMPNLDGLKASKLILNENLVDSVILLTAYSGPDFIEQAKEAGVIGYI
                     VKPVSENTLIPEIEIAISRGKELRKIKNEMNKAQMEIAKRKVIDKAKKILMKKFRITE
                     DEAYKKLRNDSMNQRITLYDLALSIIENC"
     gene            complement(1003..1308)
                     /locus_tag="VHR42_00030"
     CDS             complement(1003..1308)
                     /locus_tag="VHR42_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX3025608.1"
                     /translation="MLKGRKVGTFTAGLSMVVFGVLFLLRIYFPSINYRLILTLWPLI
                     LIFLGAEILISFFINKQEVMKYDGGAIFLTIILTFFAMGMACAEFAIQNSHYYFYWR"
     gene            complement(1301..1828)
                     /locus_tag="VHR42_00035"
     CDS             complement(1301..1828)
                     /locus_tag="VHR42_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025609.1"
                     /translation="MRKKRSKFLTVVFSFLPGAGHMFMGFMKLGVSMMGLFFLVIFLS
                     TWLSLGALIYLLPVLWFYSFFDSINKRYSTDEEFEAFEDHYLLSMDKIIGLNGTMKKN
                     ARILLGILLLLIGGYSLWTGILNNIINRLIPGPTYNALIQVMDSIPQFVLGIVIVAAG
                     IWLIMGKKKEMDNNA"
     gene            complement(1809..2270)
                     /locus_tag="VHR42_00040"
     CDS             complement(1809..2270)
                     /locus_tag="VHR42_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025610.1"
                     /translation="MNQDLFDPQNHLTDQALRAMSDGILNEEDLLAAAEHLASCTKCA
                     LNLADILEKHGLEKVPAGFTEEVKIKISQKQTEKKIQFVFYSFRVAVAACAAIALVFL
                     GTFRVPASLQSGAHRLKPADNGVVDTITSRLKNFSQQVVNLEAFYHEKEKK"
     gene            complement(2267..2779)
                     /locus_tag="VHR42_00045"
     CDS             complement(2267..2779)
                     /locus_tag="VHR42_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3025611.1"
                     /translation="MMIIRQEEFSDCIKEYEKIVITVCLSFTKNYFDAEDLAQDTFLT
                     AYRSLNTFDGGNLKAWLITIAANKCRDFLKSPKRKTGLLSPEDILYLEDNRENPAERL
                     EEKATDEKVYSLCQKLKEPYQSVATDYFCKGVGLSEQSLASGQNLRTLQTRLYRAKKL
                     LKALWKEESA"
     gene            3301..4062
                     /locus_tag="VHR42_00050"
     CDS             3301..4062
                     /locus_tag="VHR42_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX3025612.1"
                     /translation="MKLISWNVNGLRACMNKGFEQFFHDENADVFCVQETKMQKEQAD
                     FTFSGYYDYWNSAVKKGYSGTAVFSRRKPLKVSYDIGVEEHTGEGRAITLEFDSFFLV
                     NVYAPNSQRDLARLEYRISWENALRGYLSGLDQRKPVILCGDLNVAHREIDLKNPKPN
                     NGNAGFTYEEREKFSLLLEAGFVDTFRALYPEKTGAYTWWSYLFHARDHNAGWRIDYF
                     LVSEKLRSLIRDSQIYSSVTGSDHCPIGLELPDLI"
     gene            complement(4161..5582)
                     /locus_tag="VHR42_00055"
     CDS             complement(4161..5582)
                     /locus_tag="VHR42_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX3025613.1"
                     /translation="MDYRTETDSIGEKEVPKERYYGVQSLRAAENFQITGRKMCPAFI
                     ESLVQIKKAAAIANSGAGLLSRKLAGAVTQACDEIIAGGLREEFIVDPIQGGAGTSAN
                     MNANEVIANRAIELLGGKKGDYNLVNPNDHVNMGQSTNDVYPTAGKITVLKLLPELKG
                     ELVRLRKVLQEKAEEFDDVVKMGRTQLQDAVPIRLGQEFKAYASMICRDLRKIEDLDE
                     KLKVINMGGTAIGTAINADPNYFCKIVPSIEAVTGLELTQAEDLIDATQNVDVFVTVS
                     SVIKICAVNLSKMCNDLRLMSSGPRTGFGEINLPARQNGSSIMPGKVNPVIPEVVTQI
                     AYHIVGNDLTISMAAEAGQMELNAFEPIIFHDLFESISTLTGGIRTLIDHCIGGITAN
                     RQKCSELLNSSVGIVTAIAPYIGYRKAAEIAKVAISSGNSVKSVLLGKGIMEAAQINN
                     ILDPARMTRADGEQNETRHYAPK"
     gene            5972..7144
                     /locus_tag="VHR42_00060"
     CDS             5972..7144
                     /locus_tag="VHR42_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3025614.1"
                     /translation="MINRNLKIFFFMLVILGLAALGFLSYRVSINRGQMDRQVSVKTL
                     KAESELTAQRKKEASRIANENFYQKLKNGDPVSILVIGDQIAAGAGSLNDISWDGQLV
                     TLLGKSYSSDIQVTKMTSFGSNIVSSWITYNKAQQALISQGKKESTFDCVMICTGYFD
                     QTQLSYKEFHMFYENLIDRLIKANPRVVIIPILENCMKDANDYSANISELSKYYGLNL
                     IDMKAAFYNDTKPYYELVQKDKLLPNNRGYSIYASQISEDIVRNVSEDKIVNYDFSAP
                     LFESSSMLKGFQCLQTAESSGFTLSGGAYTSNRTGSSLTFTANRSVVYLEYNIAPKGG
                     KFQVYLDDTLLSTVNTNSVAPLKRQLLISNTLNETHRIKLVALSKNAVSINGIITN"
     gene            7348..8058
                     /locus_tag="VHR42_00065"
     CDS             7348..8058
                     /locus_tag="VHR42_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX3025615.1"
                     /translation="MDQGVEINIKDLLFILKKHLKFILFVTILATLAGFLVSRFILHP
                     KYEATATLIVNASTTTTNTQGLTYNDVDLSQKLVDTYAIVMKSNTILDQVIKDLSLDI
                     DVKTLANNISVEGVGTTEVMDLVVADTNPDRAKAIASDIIQLAPKEIIRTVKAGSVEI
                     ISPAKTDRTPISPNVRLNTLVAALLGLVFSYGLSFLRELLNDKFRTDEDVHKKIGFTV
                     IGVIPDITKIDEKRGYGK"
     gene            8055..>8371
                     /locus_tag="VHR42_00070"
     CDS             8055..>8371
                     /locus_tag="VHR42_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX3025616.1"
                     /translation="MSRQNKPSSIKTLLINNKAPFQFVEAYKTLRTNLQFSDIDQTVN
                     KLIVTSSLPGEGKSTVSINLAISLAQLGKTVLLVDTDLRKPLIHKYLHLSGVKNGGVT
                     TALT"
BASE COUNT         2359 a         1833 c         1749 g         2430 t
ORIGIN      
        1 ttttccccct gcaattttct tctgcttagc aattttcgat gatcgacaga gccagatcat
       61 agagtgttat tctttggttc atactgtcgt ttctgagttt tttataagct tcgtcttctg
      121 taatccgaaa ttttttcatt aatatttttt tggctttgtc aatcacttta cgctttgcga
      181 tttccatttg ggctttgttc atttcattct ttatttttct gagctcttta cccctcgaaa
      241 ttgcgatttc aatttccggt atcagggtgt tttccgaaac gggcttgaca atataaccga
      301 taacaccggc ttccttggcc tgctcaataa aatcaggccc gctgtaagcg gtcagcaata
      361 tgaccgaatc gaccaggttc tcattcagaa ttaatttaga tgctttcaac ccgtctaaat
      421 tcggcatttt tatgtctaca atggcaaggt cgggtgttaa cgcccggcac agctccaccg
      481 tctgaaagcc gtccgttgcc tgcccgacga cctcgtatcc cgcttcggtc agaatctcat
      541 agatgtccat tctggtaatg ggttcatcat ctgcaatgac gattcgatgc ttcatatcaa
      601 ttctccctgc cgttgatgac cgaacagctt ccgcggtcgt aatcctttaa tatttaacaa
      661 tataatggcc tgtcacagaa gaattgtcaa gtgatttgat ggatgcactt cttcaggtca
      721 atttcccttt agcttatgac aaaatagttt taaccaatta ggagtgtgtc tttttatccg
      781 ttgccgccaa agtgatgaat ggcgcttttc aagaaatcct cccagaatat tgtccatgtg
      841 atgaagcagg atttcaagta tttgttaacg gaatgatcat aaatatggaa taccatttat
      901 ctaaatgaag gcataattga ctgcaaaaag acgctcccaa taagggaacg tctttttacg
      961 atacctttga atgtttgaaa acctcgtact ctttgtgatc atttatctcc aataaaagta
     1021 gtaatgcgaa ttctgaattg caaattcggc gcaggccatt cccattgcga aaaaggttag
     1081 aatgatggtg agaaatatcg caccgccgtc atatttcata acttcctgct tatttataaa
     1141 aaaggaaatt aaaatttcag cacccaaaaa aatcaggatc agcggccaga gcgttagaat
     1201 cagccgataa ttaatagagg ggaaatatat tcttaataga aataaaaccc cgaaaacaac
     1261 catggaaagc ccagcggtaa atgtgccgac ttttcggcct ttaagcattg ttgtccatct
     1321 ccttcttttt gcccatgatc agccagattc cggccgcgac aatgacaatt cccaaaacaa
     1381 actggggaat cgaatccatc acctgaatca gcgcgttata agtcgggccg ggaataaggc
     1441 gattgataat attatttaag atacctgtcc aaagcgaata tccgccgatt agaagcaaca
     1501 aaattccgag cagaatccgt gcgtttttct tcatggttcc atttagaccg atgatcttat
     1561 ccattgagag caaataatga tcttcaaatg cctcaaactc ttcgtcggtg gaataacgct
     1621 tgttgatgga atcaaaaaaa gaataaaacc acaggacggg cagcagataa atcagtgcgc
     1681 caagactcag ccaggttgac aggaaaatga cgagaaaaaa tagacccatc atcgaaacgc
     1741 caagtttcat aaaacccata aacatatgac ccgccccggg caaaaaagaa aaaacaacgg
     1801 ttaaaaattt acttcttttc tttctcatga tagaaagcct ccaaattcac aacttgttga
     1861 gaaaagttct taagccttga tgtaatggta tcgacaacac cattgtcggc cggtttcaaa
     1921 cggtgcgctc cgctttgcag cgaagccgga acacgaaaag tccccaaaaa caccagagcg
     1981 atcgccgcgc aggcagcaac agccacccga aaggaataaa aaacgaattg gattttcttt
     2041 tcagtctgtt tttgactgat tttgattttc acttcttctg taaagccggc cgggactttc
     2101 tcaagcccat gtttttcaag gatatcggcc aaattgagcg cgcatttcgt gcaggaagcg
     2161 agatgctctg ccgcggcgag cagatcctct tcatttaaga ttccatcgct cattgcccgc
     2221 agggcctgat cggtgagatg attttgaggg tcaaataaat cctgattcat gccgattcct
     2281 ccttccataa ggcctttaat aatttctttg cccggtagag tcgggtttgc agcgtcctaa
     2341 gattttggcc ggaggccagt gactgttccg acagccctac gcccttgcaa aaataatctg
     2401 tggcaactga ctgataaggc tccttgagtt tttggcacag ggaatatact ttttcatctg
     2461 ttgctttttc ctccaaccgt tcagcgggat tctcacgatt atcctccaga tataggatgt
     2521 cttccggaga gagcaaacct gtttttcgtt tcggactttt cagaaaatcc ctgcatttat
     2581 tagccgcaat ggtaatcagc cacgccttca aatttcctcc gtcgaatgta ttcaggctgc
     2641 ggtaggcggt taagaacgtg tcctgcgcca gatcttctgc gtcgaaataa tttttggtaa
     2701 aagaaagaca gacggtgatg actatttttt cgtattcttt gatacagtct gaaaattctt
     2761 cctgccgaat aatcatcacc ttccttttta cccatctatt tgaataaacg attccaagta
     2821 caaaaacact tcacgattcg caatattttt caatttgcaa ataaacttta taatttttat
     2881 ctcctcatgc cgactgagat tggcaaggaa atcggaggga ttccgccaga agttgattca
     2941 agcggctaaa atgagagaat aacgctgaat cctttacggg ttttcgattt gtaatattac
     3001 ccattttgtg ctataataaa ataatcgaag tgccgatcat aacgacgctt cgcctcctat
     3061 cttccaactc gcctgttgga agaccaaagc cttgcggtgc tgccgtgagg cttttctttg
     3121 atgaacggaa aataaacgac atctttattt taccataagg tgtaactgcc cgtacagctt
     3181 cgttgtttca tccgatcaat ggaggcggcc ggaaatctat ggtataattt aaaaagagca
     3241 tgatctgccg aggccttaac agagcgggtt attcaattat tctgtaaaga cggtgaaata
     3301 atgaaactga tttcatggaa cgtaaatggt ttaagagcct gtatgaacaa gggatttgag
     3361 cagttttttc acgatgaaaa cgccgatgtt ttctgcgttc aggaaacaaa aatgcaaaaa
     3421 gagcaggccg atttcacctt ttccggctat tatgattatt ggaacagtgc tgttaaaaag
     3481 ggatactccg gaaccgcggt tttttctcgc cgaaagccgc taaaagtcag ttatgatatt
     3541 ggtgtggaag agcataccgg ggaaggcagg gcaataacgc tggaatttga cagcttcttt
     3601 ctcgtaaatg tgtatgcacc gaattcgcag cgcgatcttg caaggctgga atatcgaatc
     3661 agctgggaaa acgcgttgcg cggctatcta tcggggctgg atcagaggaa acccgttatt
     3721 ctgtgcggag atctgaacgt tgcccaccgg gaaattgatt tgaaaaaccc gaaacccaac
     3781 aacgggaatg caggttttac ctatgaggag cgggaaaagt tcagtctatt gctggaagcg
     3841 ggatttgtcg atacgttccg cgcgctctat ccggaaaaaa ccggcgctta cacctggtgg
     3901 tcctatctgt ttcacgcgcg tgatcataac gccggatggc gtatcgacta ctttcttgtc
     3961 tccgaaaaac ttcgcagcct gattcgggac agtcaaattt attcctccgt taccggaagc
     4021 gatcactgcc ctatcggcct tgaattgccg gatcttattt aatttgatca gccttcccag
     4081 acaggataaa ataaaacaag ccgctgaacc taaaaagtct cagcggcttg tttttcgtga
     4141 ttagagcggt ctgtcggaac ctattttggg gcataatgtc ttgtttcgtt ttgctctccg
     4201 tcggccctgg tcattctggc tgggtcgagg atgttgttga tttgtgccgc ttccataatc
     4261 cccttcccaa gcaaaacgct ttttacggaa tttcctgaag aaatcgccac tttggcaatt
     4321 tcggcggctt ttcggtatcc aatataaggc gcaatggcgg tgacgattcc aacactgctg
     4381 ttgagtaatt cactgcattt ttgcctgttt gcggtaattc cgccgataca atgatcgatt
     4441 aaggtgcgga tacctccggt cagggtggag attgattcaa acaggtcgtg aaaaataatg
     4501 ggctcaaagg cgttgagctc catctggcct gcttccgcgg ccattgatat ggtcagatcg
     4561 ttgccgacaa tatgataagc aatctgggta acaacctccg gaataaccgg attaaccttc
     4621 cccggcataa tggaggaacc gttttgtctg gcagggagat taatctcacc aaagccggtt
     4681 ctgggaccgg aagacatcag cctgaggtcg ttgcacattt ttgacaaatt aaccgcgcaa
     4741 atcttaatca cggaggaaac cgtcacaaac acatcgacat tctgggtggc gtcaatcaaa
     4801 tcttccgcct gagtgagttc aaggcccgta accgcttcga tggacggcac aatcttgcaa
     4861 aaatagtttg gatctgcatt aatggcggtt ccgatcgcag ttcctcccat attaatgacc
     4921 ttcagttttt catccaggtc ctcgattttc cggagatctc tgcatatcat agaggcgtat
     4981 gccttaaatt cctgtcccag tctgatgggg actgcgtcct gaagctgagt tcttcccatt
     5041 ttgactacgt cgtcaaattc ctccgccttt tcctgaagga ctttccggag ccgaaccagt
     5101 tcacctttta actcgggcag taatttgagt acggtgattt ttccggctgt gggataaacg
     5161 tcgttggttg actggcccat attcacatga tcgttggggt ttacaagatt gtagtcgcct
     5221 tttttgccgc caagcagttc aatggcccga ttggcgatca cttcgttggc attcatattg
     5281 gccgaggtcc ccgcgccgcc ctgaatcggg tcaacgataa attcctcccg aagaccgccc
     5341 gcaataatct cgtcgcatgc ttgtgtcacc gccccggcaa gttttcggct caaaagccct
     5401 gctccggaat tggcaatcgc agcggccttt ttgatctgta caaggctttc aataaatgcc
     5461 gggcacattt ttcttccggt aatctgaaaa ttttcagccg cccgaaggct ctggaccccg
     5521 tagtatcttt ctttgggaac ttctttttcc ccaattgaat ccgtttcggt cctgtaatcc
     5581 atttttatcc ccctaaaaat aagaaaaagt aattgcttta gcaatagatt ttaaggttac
     5641 aaacgattgt ccggtgaacc ggccttcgtt ctttcataat aaatcatatc gcacaaaatt
     5701 gctgtggtaa agtaattgga ttaaaccgat tgctaatatt agccacgttt aattttatta
     5761 ttttttatta agcgattcgg gcggatttct ttctttttta aaagataaga tttcttcgta
     5821 aatatatggt cttcctgtca ttaatgattg aattgcaaaa ttttatcagt tataatggaa
     5881 ccataaaaat atggtaaact tcaaaatgat tgcatcagaa ttccccggtt gcgaattgca
     5941 taaatttaaa aaactgcttg ggtgatgacg tttgataaat agaaacttaa aaattttctt
     6001 ttttatgctg gtgattctgg ggctggccgc tttggggttc ctgagttatc gtgttagtat
     6061 aaaccgagga caaatggatc gtcaggtcag cgtgaaaact cttaaagccg aaagtgaact
     6121 gacggcacag cgaaaaaaag aggcctcccg catcgcaaat gaaaattttt atcaaaaatt
     6181 gaaaaacggc gatccggtat cgatattggt aattggagat cagattgccg cgggtgcagg
     6241 cagtctgaac gatataagct gggatgggca gcttgtcact ttactcggaa agtcttattc
     6301 ttctgacatt caggtaacaa agatgacttc cttcggaagc aatattgtca gcagctggat
     6361 tacatacaat aaagctcaac aggcactcat aagccagggt aaaaaggaat ccacgtttga
     6421 ttgtgtaatg atttgcacag gatattttga tcagactcag ttatcctaca aagagttcca
     6481 tatgttctat gaaaatttga ttgacaggct gattaaagca aatccaagag tcgttataat
     6541 accgatattg gaaaactgta tgaaagatgc caacgattac tcagcaaaca tttccgagct
     6601 ttccaaatat tacgggctca atctgatcga tatgaaagca gccttttata acgatacgaa
     6661 accctattac gaactggttc aaaaagacaa gcttcttcca aacaacagag gttattccat
     6721 atatgccagc cagattagtg aggatatcgt cagaaatgtt tccgaggata aaattgtgaa
     6781 ttatgatttt tctgcgccgt tatttgagag ttcgtccatg ctgaaaggtt ttcaatgttt
     6841 gcaaacggcc gaatcttccg gctttaccct ttccggcggc gcttacactt ccaaccggac
     6901 cggatcgtca ctgactttta ccgcgaaccg gtcggttgtt tatctggagt acaacatagc
     6961 acccaaaggg ggaaaattcc aagtgtattt agacgacact cttctttcga cagtgaacac
     7021 aaactcagtg gctcccttga aaagacagct cttgatatcg aatactttaa acgaaactca
     7081 tcggattaaa ctggtcgctt tatcgaaaaa cgcggtgagc attaatggaa taataaccaa
     7141 ttgattcata gatatcccat agcctacgga acaactttta gttccgtagg ctattttttt
     7201 tatttttttc gttaaaataa aaaaaacatt ctccgttttg ttggtagatt caccaatatt
     7261 caagtttggt cgacattttt caacaaatta tgatataatt aaaaagaaat tataatatta
     7321 aaattaaagt tcacggaggc gaacgtgttg gatcaaggcg tggaaatcaa catcaaagac
     7381 cttctattca ttttaaaaaa acatcttaaa tttatcctct tcgttaccat tctggctacc
     7441 ttagccggct ttcttgtatc gcgtttcatt ctgcatccca aatatgaagc caccgctact
     7501 ctgattgtta acgcttcgac cacgaccacg aacacacagg gcttgactta taacgacgtt
     7561 gatttatctc aaaagctggt tgacacctat gcgattgtca tgaaaagcaa caccattctc
     7621 gatcaggtaa tcaaagattt gagtttggat attgatgtta aaacattggc aaataatatt
     7681 tcggttgaag gtgtcggaac caccgaggtt atggatttag tggtggcgga caccaatccg
     7741 gatcgggcca aagcaattgc cagcgatatc attcaacttg cacctaaaga aattataaga
     7801 accgtgaaag ccggatcggt tgaaattata tctcctgcaa aaaccgatcg gactcccata
     7861 tcacccaacg ttagacttaa cactttagtt gccgctctgt tgggacttgt cttcagttac
     7921 ggattatctt tcttaagaga gttgctgaac gataaattca ggactgatga agatgtgcat
     7981 aaaaagattg gtttcacggt tattggggtt attcccgaca taacaaaaat cgatgaaaag
     8041 aggggctacg gaaaatgagc aggcaaaaca agccgtcttc cataaaaacg cttttaatta
     8101 ataacaaggc cccttttcag tttgttgagg cttataaaac gttacggacg aatctacagt
     8161 tttccgatat tgaccaaacc gtcaacaaac tgattgttac gagttccctt ccgggtgaag
     8221 gaaaatcaac ggtttccatt aaccttgcta tttcactggc acaattgggg aaaaccgttc
     8281 tgctcgtcga taccgatctt cgtaaaccgc tgattcataa atatctccac ctcagcggtg
     8341 taaaaaacgg cggggtaacc actgcactaa c
//
LOCUS       DATALY010000003         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_346771,
            whole genome shotgun sequence.
ACCESSION   DATALY010000003 DATALY010000000
VERSION     DATALY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346771"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="VHR42_00075"
     CDS             complement(<1..1581)
                     /locus_tag="VHR42_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NuoF family protein"
                     /protein_id="HEX3025617.1"
                     /translation="MLKIRDDLIKMRDTYARSLAAESKKILVCAGTGCISSGSLEIFK
                     RLQTIMNEKNIPSSVELQKEPHDTVGIKKSGCHGFCEMGPLIRIEPQGWLYTKVQLSD
                     CEDIIEKTIINGELIERLAYKKNGVTYPRQDEVPFYKKQTRLVLEHCGHIDADSIHEY
                     IAIGGYAAFEKALFDMSGDQVVKTIKDSGLRGRGGAGFPAGIKWETVRKLESDQKYIL
                     CNGDEGDPGAFMDRSIMEGDPHRVLEGMMIAGIACGASEGYIYVRAEYPMAVERLRRA
                     IDQAHETGLLGTDILGSGFDFEIHINRGAGAFVCGEETALIASIEGYRGMPRVRPPLT
                     VVKGLFGKPTVPNNVETYANVPLIINRGAGWYKSIGSPQNSGTKAFALTGNIENTGLI
                     EIPMGTPLREVIYDIGGGCRNGAEFKAVQIGGPSGGCLTKENLDLPLDYDSLKSVGAM
                     IGSGGLVVMDEFTCIVEVARFFMNFTQNESCGKCVPCREGTKRMLEILENIVAGKGQE
                     GDIDLLLELADMITATSLC"
     gene            complement(1583..2059)
                     /locus_tag="VHR42_00080"
     CDS             complement(1583..2059)
                     /locus_tag="VHR42_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX3025618.1"
                     /translation="MNDSFDYKPVDGILVSLGCRQSAVIAILQAIQEHYRYLPGEIFP
                     YLAAKLNVSEARIFSVATFYENFSLKPKGKFVIRVCDGTACHVRKSVPILERLRRELG
                     LSETKVTTDDLNFTLETVSCLGACGLSPVLTVNDKVYPAMTPEKAAGLLNQLKEEL"
     gene            complement(2072..>2699)
                     /locus_tag="VHR42_00085"
     CDS             complement(2072..>2699)
                     /locus_tag="VHR42_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HEX3025619.1"
                     /translation="YDREFTAKTLCTMLLNECYSLYGGQPGDDVTVCAVKVRQRKPVN
                     LIIGPPADPADVHKMMSLFFSKEGKHIVCGGTTSQLAAGFLEKPLVTGVPLYVDPEIP
                     PTAEIEGVNLVTEGVITINRVLEYANDYICGNQKYTEWSYKKDGASLIARMLFEEATD
                     INFFVGRAVNPAHQNPTLPINFNIKMQLINELAKCLEKIGKKIKVSYF"
BASE COUNT          675 a          685 c          591 g          748 t
ORIGIN      
        1 gcaaagagag gtagctgtaa tcatatcagc cagttccagc aaaagatcga tgtcaccctc
       61 ctgacccttg cctgctacga tattttccag aatttcgagc atacgtttgg ttccctcacg
      121 gcagggaaca cattttccgc aggattcgtt ctgggtaaaa ttcataaaga aacgcgcaac
      181 ctcgacaata caggtaaact cgtccattac aacaagtcct ccggagccga tcatcgcgcc
      241 gaccgatttg agcgaatcat aatcaagagg aaggtcgagg ttctcttttg tcaggcatcc
      301 gcccgaaggc cctccgatct gcaccgcctt aaattccgcg ccgttgcggc agccgccgcc
      361 gatgtcataa attacttccc gcaggggtgt gcccattgga atttctatca gtccggtatt
      421 ctcaatattg ccggtcagag caaaggcttt cgtaccggaa ttttgcgggc tgccgatgct
      481 cttgtaccag ccggcgccgc ggttaatgat cagaggcaca tttgcataag tttcaacatt
      541 gttaggcacg gtgggttttc cgaataaacc tttaacgaca gtgagcggcg gtcttaccct
      601 tggcattccg cggtagcctt cgatggaggc aatgagcgcg gtttcttcgc cgcagacgaa
      661 tgctcccgca ccgcggttga tgtgaatttc aaaatcgaaa ccgctcccga gaatatcagt
      721 gcccagcagt cctgtttcat gtgcctgatc aatggcacgg cgcagtcttt ccacagccat
      781 aggatattcg gcacgcacgt aaatataacc ttccgacgca ccgcaggcaa taccggcgat
      841 catcatacct tccaatacgc ggtgaggatc gccttccata atgcttctgt ccataaacgc
      901 gccggggtca ccctcgtcac cgttgcagag tatgtatttc tgatccgatt ccagctttct
      961 gaccgtttcc cactttatac ctgccggaaa accggcaccg cctctgcccc gcaggccgga
     1021 atctttaatt gttttaacaa cctgatctcc gctcatatca aagagagctt tttcaaaggc
     1081 agcatagcct ccgatagcga tatattcgtg aattgaatcg gcgtcgatgt gaccgcagtg
     1141 ttcaagcaca agacgggttt gttttttgta aaaaggaacc tcgtcctgcc tgggatatgt
     1201 aacaccgttc tttttgtagg caaggcgttc gatcagttca ccgtttatga tcgtcttttc
     1261 gataatgtcc tcgcagtcgg acagctgaac ctttgtatac agccagccct ggggttcaat
     1321 tcttatcaaa ggacccattt cacaaaaacc atgacatccg cttttcttta ttccgaccgt
     1381 gtcgtgaggt tctttttgaa gctccacaga actcgggata tttttttcat tcataatagt
     1441 ttgcagacgc ttgaatattt caagagaacc gctggatatg catcccgtac ccgcgcacac
     1501 aagaattttt ttcgattccg ccgcaagcga tcttgcgtat gtatcgcgca tttttatcag
     1561 atcatcgcgt attttcagca ttttacagtt cctccttcaa ttgattcaaa agtccggcag
     1621 ctttttcggg ggtcattgcg ggatatacct tgtcatttac agtcaatacg ggagacagtc
     1681 cgcaggcgcc gagacatgaa accgtttcca aagtaaaatt aagatcatcg gtggtcactt
     1741 tggtttcgga aagaccaagc tctcggcgca gtctttccag gatcggaaca gactttctca
     1801 catgacaggc agttccgtcg cagactctta ttacaaattt ccccttgggt ttaagggaaa
     1861 agttttcata aaacgtagca acgctgaaaa tcctcgcttc gcttacattt aattttgccg
     1921 caagatacgg aaaaatttca ccgggcagat aacggtaatg ttcctgtatc gcctgcagga
     1981 tcgcaattac agcactttgc ctgcatccga gggaaacgag gataccgtca acaggcttgt
     2041 aatcaaatga atcattcata tgagtttctc cttagaaata gcttactttt attttttttc
     2101 ctattttctc aagacatttg gcgagttcgt taatcagctg catcttgatg ttgaagttaa
     2161 tgggaagagt cgggttctga tgtgcgggat taaccgcccg gcccacaaaa aagttaatat
     2221 ctgtagcctc ctcaaacagc atccttgcta tcagagacgc gccgtccttt ttataactcc
     2281 attcggtata tttctgatta ccgcagatat aatcatttgc atactcaagt acgcggttta
     2341 tagtaataac accttctgtc accagattta cgccttcaat ttcagctgta ggcggaattt
     2401 cgggatctac ataaagcgga actccggtaa ccaaaggttt ttcaagaaat ccggcagcaa
     2461 gctgagatgt tgtccctccg cagacaatat gttttccctc tttggaaaag aaaagcgaca
     2521 tcattttgtg aacatcggcg ggatcggccg gaggacctat aatcaggttt acaggctttc
     2581 tctgacggac ttttactgcg catacagtaa cgtcatcacc cggttgtccg ccatacagcg
     2641 aataacactc gttgagcagc atagtgcata atgtcttggc ggtaaattcc ctgtcatat
//
LOCUS       DATALY010000004         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_260643,
            whole genome shotgun sequence.
ACCESSION   DATALY010000004 DATALY010000000
VERSION     DATALY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260643"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..727
                     /locus_tag="VHR42_00090"
     CDS             80..727
                     /locus_tag="VHR42_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3025620.1"
                     /translation="MKKLLYITVSSKPENMSVSRQAGRAFLKSFQEDYPGYELEELDL
                     ATYDLPEPNYRYFKGWVELVSGAEYDALPEPDQEAVDRMNSLCTQFLAADITVIAAPM
                     WSMSFPFRLKQYLDCIMLNNRLIRLTPTEATGLLNDKVRKMVYIQSSGGIYPKIFDGK
                     FNMGIKYFHDLFLHLGVKDFYKILIQGTDMVDVGKEKALQAAQDDFDDVLDKICG"
     gene            920..1420
                     /locus_tag="VHR42_00095"
     CDS             920..1420
                     /locus_tag="VHR42_00095"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX3025621.1"
                     /translation="MHSKISIGLTRFLTAVVAALTLCDAILTVTAPWWLTLINKISYQ
                     DLRIMLGGIFQGARSNGSYPVYLSFTILCGLLSLGILISALRILTRIRQNDPFCFRNA
                     DNFKNAAFFAFALSLIFLMKMTFSASILTLICAGVFLLGGLFLMVLSELFQTAARIKE
                     ENELTI"
     gene            1440..>1579
                     /locus_tag="VHR42_00100"
     CDS             1440..>1579
                     /locus_tag="VHR42_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3025622.1"
                     /translation="MPIIVNLDVMMAKRKISLSELASRVDITLANLSVLKNNRAKAVR
                     FST"
BASE COUNT          435 a          362 c          339 g          443 t
ORIGIN      
        1 gacaaaaagc tcataatata atggattaat tgcagccgga cgaaaacaca ccggctcttt
       61 ccagtaagga gcctaaatta tgaaaaaatt attatatatc actgtcagca gcaaaccgga
      121 aaatatgtcc gtcagcagac aggccggccg ggcatttcta aaaagttttc aggaagatta
      181 ccccggttat gagcttgaag agctggatct tgccacatac gatcttcccg aaccgaacta
      241 tcgctatttt aaaggctggg tggaacttgt cagcggagca gaatacgatg ccctcccaga
      301 accggatcag gaagcggtag accggatgaa cagtctctgc acgcaatttc ttgccgccga
      361 tattacggta atcgccgcgc cgatgtggag catgagcttt ccgttcaggc taaaacaata
      421 ccttgactgc atcatgctca ataaccgcct catccggctg actcctaccg aagctaccgg
      481 tcttctcaat gacaaagtaa gaaaaatggt ttatatccag tcctccggcg gaatttatcc
      541 aaaaatcttt gacgggaaat ttaatatggg cattaaatat tttcacgatc tcttcctgca
      601 tctcggcgtc aaggactttt acaagattct gattcaaggt acggatatgg tcgacgtggg
      661 aaaagagaaa gccctccagg ccgctcagga tgattttgac gatgtactgg acaaaatctg
      721 cggctgacac attatatttt cttcataaag cagcatttgt aaaacaggtt ccttccgcgg
      781 aaggaacctg tttatctttt tacattatat atttatcgtt aatcgataaa aacatattga
      841 catataataa gctgaatgat aaaatgtgtc aaagagaaac cggtttaaaa gacaatcatg
      901 atttaaagag gggatattga tgcattcaaa aatttcaatc ggccttaccc gattccttac
      961 ggctgtcgtt gccgcgctta cactttgcga cgcaatcctg accgtcaccg ctccctggtg
     1021 gctgactctg attaacaaaa tcagctatca ggatctgcgt atcatgctcg gcggaatctt
     1081 tcagggcgcg cgcagcaacg gcagttaccc ggtctatctg tcatttacga tactttgcgg
     1141 gctgctcagc ctgggcattt taatttcggc gctgaggatt ctcacccgaa ttcgccaaaa
     1201 tgaccctttt tgcttccgca acgccgacaa ctttaaaaat gcggcatttt ttgcctttgc
     1261 cctatcactg atttttttga tgaaaatgac cttttccgct tcgattttga ctctgatctg
     1321 cgccggagta ttcctgttgg gcgggctttt ccttatggtt ctgtcggaat tatttcagac
     1381 cgccgcacgc attaaagaag aaaatgaact gacgatatga atttttttgg ggtgatacaa
     1441 tgcctattat cgtaaatctg gacgttatga tggccaaaag gaaaatatct ctgtcggagc
     1501 ttgcctcccg tgtggatatt acgctcgcga acctttctgt tttaaaaaac aatcgggcga
     1561 aagccgttcg tttttctac
//
LOCUS       DATALY010000005         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_87837,
            whole genome shotgun sequence.
ACCESSION   DATALY010000005 DATALY010000000
VERSION     DATALY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87837"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1747)
                     /locus_tag="VHR42_00105"
     CDS             complement(<1..>1747)
                     /locus_tag="VHR42_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3025623.1"
                     /translation="LRLQEPISTLYYEGRVGQASYSCPDGIINGEILLKRQFEIENRR
                     LLSYNDIDITFDEELLKPYLSVSSNTRLKNIIATIQSEQNRIIRAGLLKPLIVQGAAG
                     SGKTTVALHRIAYLIYATTIKPEQFLIIAPNAIFLDYISNVLPDLGVENVHQKTFDSF
                     AQNFIGKKLKIEDPGEKLNRILDEKNADEEISASGFKSSLLFRDVIERYLKDLEKEQL
                     PREHFCVGRHVLCSYGKIFGLFTKEYAGFSFSKRMNFIKERLVNLIADNANRVIAKID
                     VERRLRISELPDSLSAEEYKKQKQQIYEQDEDAVHDLLTGGKNALKAYFKKIKLPTAL
                     ECYHRLISDPEILKKYASTANTEILSFLSSRSQKIKTGYVEYEDLAPLMYLHYRIHGV
                     KQGLSIRHVIIDEAQDLSAFQFFVLKEVLECSSMTILGDIAQGIYSYRGTGNWGSVAD
                     KIFGSDCEYATLQKSYRSTVEIMGQANRVISKLSGFQESAAAEPVIRNGDPVRYIKAN
                     SEKEIVAGIAERIKQLRGKGFKNISVICKTMRGCEAFAKLLKKYISEFTVISGKEYSG
                     GVSIVPSYLVKGLEFD"
BASE COUNT          423 a          421 c          393 g          510 t
ORIGIN      
        1 cgtcaaattc aagacctttt acgagatagg acggcacaat gctcactccg ccggaatatt
       61 cttttccgct tatcacagta aattccgaaa tgtatttttt cagtaatttt gcaaaagctt
      121 cgcagccccg catggtttta cagataaccg aaatgttttt aaagccctta ccgcgcagtt
      181 gcttgatacg ctctgcgata ccggcgacga tttccttttc gctgtttgct ttgatatagc
      241 gtaccgggtc gccgtttcga attaccggct cggctgctgc ggattcctga aacccgctga
      301 gcttcgaaat aaccctgttt gcctgcccca tgatttctac cgtggaacgg tagcttttct
      361 gcagggttgc atattcgcag tctgagccga aaatcttatc ggcaacgctg ccccagttac
      421 cggttccgcg gtaagagtaa attccctgtg cgatatcgcc cagtatcgtc attgagctgc
      481 actccagaac ctcttttagc acaaaaaact ggaatgcact taaatcctgc gcctcgtcga
      541 tgataacatg ccgaatcgaa aggccttgtt ttacgccgtg gatccggtag tgaagataca
      601 ttaatggcgc aaggtcttca tattccacat agccggtttt tatcttttgc gaacgggatg
      661 ataggaacga aagaatttct gtatttgcgg tgcttgcata ctttttcagg atttccggat
      721 cggatatcaa cctgtgatag cattcaagag ccgtcggcag ctttatcttc ttgaaatatg
      781 ccttcagagc atttttccca cctgtaagca gatcatgcac ggcatcctcg tcctgctcat
      841 aaatttgctg cttttgcttt ttatattcct ccgctgaaag actgtcgggc agctccgaaa
      901 ttcggagcct tctttcaacg tcgattttag cgataacccg atttgcgttg tcggcgatga
      961 ggttgacaag ccgctctttg atgaagttca tccgttttga aaaggaaaat ccggcgtact
     1021 cttttgtgaa cagaccaaat atttttccat aactgcacaa gacgtgtctg ccgacgcaaa
     1081 agtgctcacg cggaagctgt tccttttcga gatcctttaa gtaccgttcg atcacgtcgc
     1141 ggaacaaaag cgacgacttg aatccggaag cggaaatttc ctcatccgca tttttttcgt
     1201 caagaatccg gtttagtttt tcaccgggat cttcgatttt gagctttttt ccgataaaat
     1261 tctgcgcaaa agaatcaaag gttttctgat gaacgttctc gacgccgagg tccggcagca
     1321 cattggagat atagtccaga aaaattgcgt tcggggcaat gatcaaaaac tgctccggtt
     1381 taatagttgt ggcgtagatg agatacgcga tccggtgcaa cgcaacagtg gttttaccgc
     1441 ttccggctgc gccctgcaca atcagcggtt ttaaaagccc cgcacgtata atcctgttct
     1501 gttcggattg tatcgttgcg atgatgtttt tcagccgtgt gtttgagctc acggaaagat
     1561 acggctttaa aagctcctca tcaaaggtga tatcaatgtc gttatagctg agaagcctgc
     1621 ggttctcaat ctcaaactgc cgttttaaaa gaatctcacc gttaataatc ccgtcagggc
     1681 aggaatagga agcctgcccc acgcggcctt cgtaatataa tgtggatatg ggttcctgca
     1741 gtctaag
//
LOCUS       DATALY010000006         9096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_433703,
            whole genome shotgun sequence.
ACCESSION   DATALY010000006 DATALY010000000
VERSION     DATALY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433703"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VHR42_00110"
     CDS             <1..197
                     /locus_tag="VHR42_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025624.1"
                     /translation="FLNKAVFIISFSLTVLAFIYGWVSGQTNNIFDLIIGAIINFIIW
                     YLIVYFVFWLVKRKRRDDKV"
     gene            558..2081
                     /locus_tag="VHR42_00115"
     CDS             558..2081
                     /locus_tag="VHR42_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3025625.1"
                     /translation="MDKIEPYAKSVFAYLRKSRAEENENTEEVLARHKAELTRFATKH
                     RLNIKKWYEEVVSGDSLFVRPRMIELLHDVEDGKCTGVLCMDIDRLGRGNMQEQGLIL
                     NTLKDADTVIITPDKVYDLNDEQDETQTEFKTFFARQELKMIKKRLSRGVRMTIEKGG
                     YVSNVPFGYARDYKDKIPTLAPHPEESEYVRMIFNLYIQGDGCQTIAYKLKNMGVKPH
                     RGNEFNRTSIRKIITNPVYIGKIVWGQKKHLRPKAIGEKHRTVYLPKEDWMIYDGLHP
                     AIIDEDIFRKANDMLRSKYHVPYRESDQIVNPLAGLLFCRQCGKAITRRPFKNRKYQT
                     THMICTTLGCVKSARADYVEEDFLEALRGEYKKLIEQQSEEMKEKRSEQTQILLSGMK
                     VEISRLLKQKERLYDLLEQGVYSIETFTEREMNLLNRINGLSKEIKEAEAAELKRNRR
                     AEILIPQIKNVLQNYWEGTPSDKNNLLKGVVEKAFYFKDKEAGPKDFTIEMELRKDL"
     gene            2620..3567
                     /locus_tag="VHR42_00120"
     CDS             2620..3567
                     /locus_tag="VHR42_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3025626.1"
                     /translation="MKTKGILKIIAGAAVTVISVVFAVKALGDVDFYAVFHAQINWGM
                     AVVSAVLFVLSTFFRGLAYTRGIDRNLTAMQSWRIVAVGNASNLFLPLRGGEGIRFAL
                     FPEGYSAIERTRLTMVPGVADIAAILAVSLSAVIFADFRDPVLVDWLNLIAAVFVSVV
                     FVVVLVLYILPKTRSAVRRHMSGDVLMMSFWVALSWVIVLLSIWVGILSFGADPNGSG
                     DMTIAVFAGTSLALFIPSSPGGIGVFEYSVILSLGSLGMPPAPAKVIGILLHLIQYAS
                     LLPAGIILYLTGMDPNKRKKFRTGFRKRRRRRPAALLKK"
     gene            3649..4386
                     /locus_tag="VHR42_00125"
     CDS             3649..4386
                     /locus_tag="VHR42_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX3025627.1"
                     /translation="MPKIKVLNIEFDNLTLDEAAGRVMEFAQGDTPRIVVTPNAEIAE
                     CCYQNEQLSRAVAAADMAVPDGISVVMASKILGRPLKTRLPGIDLARMLFPMMIRKNL
                     SLYLLGAKPDVAAKAAKNLQTEFPGLRIAGIQHGYFKNDDAVIEAVNRSGADVLFVAM
                     GFPRQEIWMAENRDQLRVGVMLGLGGSLDVFAGEVRRAPDFFIRLNLEWLYRLIKQPS
                     RFGRMLKLPRYLLRAIGVRIFGGEQRK"
     gene            4426..5202
                     /gene="thyX"
                     /locus_tag="VHR42_00130"
     CDS             4426..5202
                     /gene="thyX"
                     /locus_tag="VHR42_00130"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX3025628.1"
                     /translation="MKVELLAYTPEPEKIVAAAAKLCYSPSSISDITEGLTEEKTASF
                     VDMLSQIGHESPIEHASFTFGVEGVSRSLLAQLTRHRIASFSVQSQRYVREDCFEYVI
                     PPEIRGIPRAEAEFIAAMKEDQRHYDSLAKMLQEKHYQEFLRRGEDEKSAARKAEKAA
                     IEDARFVLPNACTTKIIMTMNCRSLIHLFRLRCCNRAQWEIRELTVAMYRLVVQVAPH
                     LFAKAGPSCVDGSCSEGKMSCGRADEVRETFRCLRNSAKQ"
     gene            5219..5812
                     /locus_tag="VHR42_00135"
     CDS             5219..5812
                     /locus_tag="VHR42_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX3025629.1"
                     /translation="MGKIFCLMGKSGSGKDTVFRRLLKERGLGLKAVVTYTTRPRRQN
                     ERDGVEYYFITCDELNQFRDQGRIIEIRRYDTVQGAWYYCTVDDGQIDLDSGNYLMIV
                     TLEAFNSLKRYFGAEKVVPLYLFVDDGERLTRAIAREKRQPGPDYSELCRRFLADAAD
                     FEDQKLAAAGIHQSYANDNLDDCADKICRAIAEDIAH"
     gene            6005..6544
                     /locus_tag="VHR42_00140"
     CDS             6005..6544
                     /locus_tag="VHR42_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HEX3025630.1"
                     /translation="MVYLFLAEGFEEAEAVAPADILRRAGAEVRLVGVGGLLVKGAHG
                     IGITADLKQDEAEKEGLEMIVLPGGMPGTANLENDAVVQGLIEYAYEKGLWIGAICAA
                     PSILGHKGFLKGKKAVCYPGYEKDLEGARVLKTATCEDGNIITANGPGAAMEFGILLA
                     SKLKGTGRSSQIRNSMMMK"
     gene            6545..7192
                     /locus_tag="VHR42_00145"
     CDS             6545..7192
                     /locus_tag="VHR42_00145"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX3025631.1"
                     /translation="MNVYFRKKELRQSLREIRRRLSPEEKARLDQSVFENLIRTEEYR
                     KAQAILCYVSVRDEVDTLRVIGHALETGKKVAAPICIPEGNRMEFHLISSIDDLKIGH
                     FGLLEPEPKPQTLFYPAKLDFCIVPGLSFDRLGGRLGYGGGYYDRFLSKYRITAAGIC
                     YSSLISKIILPTGRYDVPVSIVVTDRAVIRTGHIPGGRGRRSRNGGANNQLKKAP"
     gene            7456..8916
                     /gene="mltG"
                     /locus_tag="VHR42_00150"
     CDS             7456..8916
                     /gene="mltG"
                     /locus_tag="VHR42_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX3025632.1"
                     /translation="MEKDQNSQTNAVPESEPRETEKNQDMVRKKAGPDTNDVGDRQDG
                     EPGGAAPAQREKPDECTELTEQPQPGQADNGGENPDEDKNGDLPDQKETSPDGQETAG
                     PSEQSGEPEDTSGADNGDGSMTATGFFKGKSKKKGCLIYSLIITVVSVLLAFFILLGI
                     NDMAGFIKPDKVIRVSIPAGASNGQVADQLKKDGVISNDLLFKFYLKVKKEGGFQRGT
                     YDLNSSMGYAQIVETMKSAANNKAVVRVVVPEGYTLRQIGETLESKKVCKKSAFLSAV
                     ESAGIKFDYMSDVPYSQNRFYHLEGYLFPDTYEFYTGESATSVAQIMLKNFKSKFNSA
                     YLARAKQINMTMDQVVTLASIIQAEASKTDEMSKVSSVFHNRLTHGVDGRKVLESDAT
                     IFYVKRNIQPVLKDDSKYELAYNTYKHEQLPPGAICNPGIAAIKAALYPDTTSYYFFV
                     TDSENHYYYSQTYAQHQIAVRKAMKSGSAIGTDVGN"
BASE COUNT         2457 a         1996 c         2427 g         2216 t
ORIGIN      
        1 aatttttaaa taaagcagta tttataattt cattttctct cacagtactt gcttttattt
       61 atggctgggt atcgggacaa acgaacaaca tttttgattt gattataggt gccataataa
      121 attttataat atggtatcta attgtttatt ttgttttttg gcttgttaaa agaaaaagaa
      181 gagatgataa agtatgaaaa agattttgtt cgtggttttt gtagtgttat acatagcgtc
      241 aatttcttta acaacgatat ttttcattcc aaaatatgct gaatatagag atggaaacat
      301 gaagcaacgg atatcaaccg gatattatag aatttacaaa acaactgttg ctacggacag
      361 aagagatcaa ctatatcaac ggattactga aaacttacaa atagacacgg gacaatggtt
      421 gattatcaca tttgctgaaa gcgtaatttt cttactgccg gcaatgttat tttttaagaa
      481 tagtaaaaat aattaataat ggagtaaact aattaagccc cggattactc cgaggctttt
      541 aatttaaggt gggtaaaatg gataaaatag agccttatgc aaaaagtgtc ttcgcatatc
      601 tccggaaatc cagagcggaa gaaaatgaaa acaccgaaga agtattggcc cggcacaagg
      661 ccgagctgac gcggtttgct acgaagcaca gattaaatat taaaaagtgg tatgaagaag
      721 ttgtcagcgg agacagcctt ttcgttcgtc ccagaatgat tgaactgctg catgatgtgg
      781 aagacggaaa atgtaccggt gtgttatgca tggatattga ccgtctcggg cgcggaaata
      841 tgcaagaaca aggattaatc ctgaatacgc ttaaagacgc tgacacggtt attataacgc
      901 ctgacaaggt atatgatctg aacgacgagc aggacgaaac gcaaacggaa ttcaaaactt
      961 tctttgcccg gcaggaactt aaaatgatta aaaagcggtt gagtcgcggc gttcgaatga
     1021 ccatcgagaa gggcggttat gtatcaaacg tgcctttcgg gtatgcgagg gactacaaag
     1081 acaagattcc gacgctggcg ccgcacccgg aagaatcgga atacgtccgg atgatcttta
     1141 acctgtatat tcagggtgac ggctgtcaaa caatcgccta taagctgaaa aacatgggag
     1201 tgaaaccgca tcggggaaac gaatttaacc ggacgagtat ccgaaagatc attaccaacc
     1261 cggtttatat cgggaaaata gtctggggac agaaaaagca tttaaggccg aaagcaatcg
     1321 gagaaaagca ccggactgtg tatttgccaa aagaagattg gatgatttat gacgggctgc
     1381 atccggccat aatcgacgag gacatttttc gtaaggcaaa cgatatgctt cgcagtaagt
     1441 atcatgtgcc atatcgggaa agcgatcaga tcgtcaatcc tcttgcggga ctcctgtttt
     1501 gccgacaatg cggtaaggcc attacccgcc gaccgttcaa aaatcgaaaa taccagacaa
     1561 cgcacatgat ttgcacaacg cttggctgtg tgaaatctgc ccgggcagat tatgtggaag
     1621 aggatttcct tgaagccctt aggggagaat ataaaaagct gattgaacag caatccgagg
     1681 aaatgaagga aaagaggtcg gaacagaccc agatcctttt aagcggaatg aaagtcgaaa
     1741 tttcccggct gctgaaacaa aaggaacggc tttatgattt gctggagcag ggcgtttaca
     1801 gtattgagac gttcaccgag cgggaaatga atttactcaa tcgtattaac ggcttgtcca
     1861 aagaaatcaa agaggcggaa gctgcggagc tgaaacgaaa caggagagcg gaaattctga
     1921 taccacagat aaaaaacgtc cttcaaaatt attgggaggg aacgccctcc gataaaaaca
     1981 atttgctcaa aggcgttgtt gaaaaggcat tttattttaa agataaagag gccgggccaa
     2041 aggattttac gattgaaatg gagttaagga aagacctgta atatctttat atacaggatg
     2101 aacattcttg ttcgctgcag gaatcataaa cgcggcccgc atcgatacaa acggcttctt
     2161 tgaaggtttg atgtccaccc gcgttcgcgg cgggcgaaac cttactgtcc accataagta
     2221 tcctcctaag cattgttctg aagtaaactt caatactata ttatgaatat tccaaacttg
     2281 tgtgaaagcg acgggctgta aataatggaa aatacgcccg gcgttttcgg tatgctttac
     2341 aaaacaatgc cgcttcaatc tgcagacggg ctgtatcgcg gcggctttcc tgtccgaatg
     2401 aaatttttga tttattacca ttatatgctc cgagcgccaa tgttatcttc tgcaattcaa
     2461 acatcttctg attgatcatc cgggctgtga ctgaaacggt cgcggcgcgg ttataattca
     2521 tccacattcc agtataatag tactgaactg taacgaaccg gtttctccgg atttttttcc
     2581 ggtgccttcc gcagtaatct tttgatcgag agagttcata tgaaaacaaa aggtattttg
     2641 aaaataatag ccggcgcggc tgtaacggtc atttctgtgg tgtttgccgt gaaagcgctt
     2701 ggcgatgtgg atttttacgc tgtttttcac gctcagatca actggggcat ggctgttgtt
     2761 tcggctgtcc tttttgtttt atccaccttt tttcgcgggc tcgcctacac gcgcggaatc
     2821 gaccgtaacc tgacagccat gcagtcatgg cggattgtag ccgttggcaa cgcttccaat
     2881 ctgtttctgc ccctgcgcgg cggtgaagga attcgttttg cccttttccc ggaaggatac
     2941 agcgcgattg agagaaccag gctgacgatg gttccggggg tggctgatat tgcggccatt
     3001 ctggccgttt cgctgtcggc cgtaatcttt gcggatttcc gggacccggt tttggtggat
     3061 tggctgaatc tgattgcggc tgtttttgtc agcgtggttt ttgttgtggt gctggttctt
     3121 tacattttgc cgaaaacgcg gtcggcggtc agaagacata tgagcggaga cgtcttaatg
     3181 atgtcgttct gggtcgctct gtcctgggtg atcgttttgc tttccatctg ggttggaatc
     3241 ctatcttttg gggccgatcc caatggctca ggggatatga cgatcgccgt tttcgcggga
     3301 accagtctcg cgctgtttat tccgtcctct ccgggaggaa tcggcgtgtt tgagtattcc
     3361 gttattctca gtctcggcag tctcggtatg ccgcccgctc ccgcgaaggt catcggcatc
     3421 cttctgcacc tgattcagta tgcctcgctg cttccggcgg gaatcatcct ctatctcacc
     3481 ggaatggatc cgaataagcg aaaaaagttt cgaacgggtt tcagaaagcg gaggagaaga
     3541 aggccggctg ccttattgaa aaaatagctg tctgtgatat aatgtgctat aatggataaa
     3601 aatactacac cgttggactc ttaaaaagat agtctgaccg aggaaactat gcctaaaatc
     3661 aaagtactga atattgaatt tgacaatttg acattagacg aagctgccgg acgggtcatg
     3721 gaatttgcgc agggcgacac gcccaggatt gtagtgacgc cgaatgcgga aattgctgaa
     3781 tgctgttatc agaacgagca gctcagccgg gcggtagcag ctgcggatat ggcggtaccg
     3841 gacggcatca gcgtcgtcat ggcgtccaaa atacttggac ggccgttgaa aacgcgtttg
     3901 cccggaattg atctggcgag gatgctgttt ccgatgatga tccggaaaaa cctgtcgctt
     3961 tatcttttgg gtgcgaagcc ggatgtagct gccaaagctg caaaaaatct tcaaacagag
     4021 tttcccggtc tccggatagc gggcattcag catggctatt ttaaaaatga cgatgcggta
     4081 attgaagcgg tcaaccgttc gggggcggac gttttgtttg ttgcgatggg atttccgagg
     4141 caggaaatct ggatggccga aaacagagac cagctccgtg tcggggtgat gcttggtctt
     4201 ggaggaagcc ttgatgtgtt cgccggggaa gtcaggcgcg cgccggattt ttttatacgg
     4261 ctgaatctgg agtggctata ccggctgatc aaacagccgt cgcggtttgg ccggatgctc
     4321 aagctgcccc ggtatctgct ccgggcgatc ggtgtacgga ttttcggcgg agagcagcgg
     4381 aaatgagaaa acagtgggcc gcaaggcggc cggaggtaga aaaaaatgaa ggttgaactg
     4441 ctggcgtata cgccggagcc ggaaaaaatt gtggcggcgg ccgcaaaatt gtgttattcc
     4501 ccaagttcca tttcggatat taccgaaggg ctgaccgaag aaaagacggc ctcgtttgtg
     4561 gacatgctct cgcaaatcgg gcatgagagc cccattgagc acgccagctt tacttttgga
     4621 gttgaaggag tgtcccggtc gttgctggct cagttgaccc gtcacagaat cgcatccttc
     4681 agcgtccaaa gccagcggta tgtccgggaa gattgctttg aatatgtgat tccccccgaa
     4741 ataagaggca tcccacgggc ggaagccgaa tttattgccg cgatgaagga agaccagcgt
     4801 cattacgaca gcctggctaa aatgcttcag gaaaagcatt atcaggaatt tctccggcgc
     4861 ggggaagatg aaaaatcggc cgcccgcaag gcggaaaagg ccgcgattga agatgcgcgg
     4921 tttgttcttc caaacgcctg cacgaccaaa atcattatga ccatgaactg ccgcagcctg
     4981 attcaccttt tcaggctccg ctgctgcaat cgggcgcagt gggaaatccg ggagctgacc
     5041 gttgccatgt atcggctggt tgtccaggtg gcgccgcatc tgtttgccaa ggcgggcccc
     5101 tcctgtgtag acggctcctg ctccgaaggt aaaatgagct gcggcagggc ggacgaagtc
     5161 cgggaaacct tccgttgtct gcggaattcg gccaaacagt aaaggaattg tgttgcatat
     5221 gggcaaaata ttttgcttaa tgggaaaaag cggctcgggc aaagatacag tattccgccg
     5281 ccttttgaaa gaacgcggcc tcggtctgaa agctgttgtt acatacacaa cgcggcccag
     5341 acggcagaac gagcgcgacg gtgtggaata ttattttatt acctgcgacg agctgaatca
     5401 atttcgagat cagggccgga tcattgaaat acgccgttat gacaccgtac agggcgcgtg
     5461 gtattactgc acggtagacg acggccagat tgatcttgat tcggggaatt atctgatgat
     5521 agtcacactc gaagcgttca acagtctcaa acgatatttc ggggcggaaa aagtagtccc
     5581 gctttatctt ttcgtcgatg acggggagcg gctgaccaga gccatcgcaa gggaaaaacg
     5641 gcagccgggc ccggattaca gcgagctttg ccggcgtttt ctggcagacg ctgctgattt
     5701 tgaagaccaa aagcttgccg ctgcggggat tcatcaaagt tatgcgaacg ataatcttga
     5761 cgactgtgcc gacaaaatct gccgggcgat tgccgaagac atcgctcatt aaggattgaa
     5821 cagacaaact gtttattttg tctgttgggg tttatactat tacggtggtc tctcaataga
     5881 gcgcattatg aaaaaagagg gtcccttaat caacaatgat caaccggcag ggataaattc
     5941 ttgggtatac acggccggaa tgttagacgg atggtaaatt ctcagtcaga cagggaggat
     6001 tgtcatggtt tatttgtttt tagccgaagg atttgaggaa gccgaggcgg tggcgccggc
     6061 cgacattctc agacgcgccg gggcggaagt ccgtttagtc ggcgtcggag gcctgctggt
     6121 caaaggagct cacgggatcg ggatcacggc ggatcttaag caggatgagg cggaaaaaga
     6181 gggattggaa atgattgtcc ttcccggcgg catgcccggc acggccaatt tggaaaacga
     6241 cgccgtcgtt cagggcttga tcgagtacgc ctatgaaaaa ggtctttgga tcggagcaat
     6301 ctgcgccgcc ccctccatat tgggtcacaa aggatttctc aagggaaaaa aagccgtctg
     6361 ttatcccgga tatgagaaag acctcgaagg cgcccgggtt ctgaaaacgg cgacctgcga
     6421 agacggaaac attattaccg caaacggccc tggagcggcg atggaattcg gtattttgct
     6481 cgcttccaag ctgaaaggga cgggaagatc ctcacaaatc cgaaactcga tgatgatgaa
     6541 ataaatgaat gtttactttc gaaaaaaaga gctcagacaa agtctcagag aaatccggcg
     6601 gcggctttct ccggaggaga aggcccgcct cgatcaatcg gtttttgaaa acctgatccg
     6661 gacggaagaa taccggaagg cgcaggcaat cctatgctac gtttcggttc gtgacgaggt
     6721 ggataccctg agggtgattg gccacgcgct cgaaacaggt aaaaaagtcg ctgccccgat
     6781 ctgcatcccg gaaggaaacc ggatggagtt tcatttgatt tcttccatcg acgatctcaa
     6841 aatcggccat ttcgggcttt tggagccgga gccgaaacca caaacacttt tttatccggc
     6901 caaattggat ttctgtatcg taccggggct ttcttttgac cgtcttggcg gccggctcgg
     6961 ctatggcggc ggatattatg accggttttt gtcaaaatac cggatcactg ccgccggcat
     7021 atgctattcg tcactgatca gcaaaatcat ccttccgaca gggcgctatg atgttcctgt
     7081 ttctatcgtg gtgaccgacc gggcagtgat ccggaccgga catataccgg gcggccgagg
     7141 acgtcgttcc cggaacggcg gcgcaaataa tcaattaaaa aaagcccctt aaatcggggc
     7201 ttttcagtat tcagagatgt agtccggtca gcagcaaccg tctttgcagc agggcctgtt
     7261 gttgttgttg ttgcacaggc aaagaaaaat tacagcaagt atgataatac attcccagtt
     7321 atttccccat ccattaaaac acatttcttt cacctcttgt tggttttgtt ccatcttatg
     7381 aaaaccggga cagaatcgtt acagtaaaat gcagaccccg gaaggtccgg actacatagg
     7441 aatagagggt aatttatgga gaaggatcaa aacagccaga ccaacgcggt tccggagtcg
     7501 gaaccgcggg aaaccgaaaa aaatcaggat atggtccgga agaaagctgg gccggatacg
     7561 aatgacgtcg gtgacaggca ggacggggag cccggcggtg cggcgccggc tcaacgtgag
     7621 aagccggatg agtgtaccga attaacggaa cagccgcagc ccgggcaagc cgataacggc
     7681 ggggaaaatc cggatgaaga caaaaacggc gatttgccgg atcaaaagga aacttcgccc
     7741 gacgggcagg aaacggccgg gccgtcggag cagagcggag agccggagga tacttccggg
     7801 gcagataacg gcgatggctc tatgacggcg acgggctttt tcaagggaaa atcaaagaaa
     7861 aaggggtgcc tgatctactc tctgattata actgtcgtat cggtgctgct ggcatttttc
     7921 attcttctgg gaataaacga tatggcaggc ttcatcaaac cggataaagt gatccgcgtt
     7981 tctattcccg cgggagccag caacggacag gttgccgatc agctgaaaaa agacggggtt
     8041 atatcaaacg atttgctttt taaattttat ctgaaggtca aaaaggaagg cggatttcag
     8101 cgcggaactt atgacctgaa ttccagcatg ggatacgccc agatcgtcga aacgatgaaa
     8161 tctgccgcaa acaacaaggc cgtcgttcgg gtggtagtgc cggaaggcta cactctgcgc
     8221 cagattgggg aaacgctgga gtcgaaaaag gtctgcaaaa aaagtgcgtt tctgagcgcg
     8281 gtggaaagcg ccggtatcaa atttgattat atgtccgatg taccgtacag ccaaaaccgg
     8341 ttttatcatc tcgaaggcta tttgtttccc gatacttacg agttctatac cggcgagtct
     8401 gccacgtccg tagcgcaaat catgctgaaa aattttaaaa gtaagttcaa ctccgcctat
     8461 cttgccaggg caaaacagat caatatgacc atggatcagg ttgtgacgct cgcctcgatt
     8521 attcaggccg aagcaagcaa aaccgatgaa atgagcaagg tttcttcggt ttttcacaac
     8581 cggctcacac acggagtgga cggccgaaag gtattggaat ccgacgcgac aattttttat
     8641 gtaaaaagaa atattcagcc ggttttgaag gacgattcca aatatgagtt ggcgtacaac
     8701 acctacaagc atgagcagct gccgccgggc gcaatctgca atcccggaat cgccgccatt
     8761 aaagccgctc tttatcccga tactaccagc tactacttct ttgttaccga tagtgaaaat
     8821 cactactatt actcgcagac ctacgcccag catcagattg ccgtgcgcaa ggccatgaag
     8881 tccggctccg cgattggtac cgatgtagga aactgagaag gaaggtcatc atgaaaaagc
     8941 ccgaactgct ctcaccggcc ggtgattttg aaaggctggt catggctgtc cattatgggg
     9001 aaataactgg gaatgtatta tcatacttgc tgtaattttt ctttgcctgt gcaacaacaa
     9061 caacaacagg ccctgctgca aagacggttg ctgctg
//
LOCUS       DATALY010000007         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_1151,
            whole genome shotgun sequence.
ACCESSION   DATALY010000007 DATALY010000000
VERSION     DATALY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..101)
                     /locus_tag="VHR42_00155"
                     /pseudo
     CDS             complement(1..101)
                     /locus_tag="VHR42_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
     gene            222..476
                     /locus_tag="VHR42_00160"
     CDS             222..476
                     /locus_tag="VHR42_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX3025633.1"
                     /translation="MSLAHSALIQSYQGRSVGRSGLQAGDLVFFDTNGGGGSINHVGI
                     YVGGGRFVQACSRNPNAITVSSLNERYYQRTYVTARRILN"
     gene            complement(608..1483)
                     /locus_tag="VHR42_00165"
     CDS             complement(608..1483)
                     /locus_tag="VHR42_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3025634.1"
                     /translation="MVIKLNYKIITVIFLILFILFLSPSLLNIGKHPVTAAETSTDAP
                     IRIPIILYHEIRPSHLNNMSITPWDFESDLKFLKKSGYTAINMSDLIGYVNGKGSLPD
                     HPVMITFDDGYLNDYVYAYPLIKKYGVKIVLSVIGKNTEDFSNVSDSNLDYAHVTWKQ
                     LNEMSDSGFIEIQNHTYNLHSITRKRFGCEKSKSESVGQYQDLLRNDLSKCQNLISSN
                     TGQLPTTFAYPYGSISKEALPVIKSLGFQATFSCSYGINKITKNPAELYGLKRITRYH
                     GRPLQRTMKECDKLK"
     gene            1728..1955
                     /locus_tag="VHR42_00170"
     CDS             1728..1955
                     /locus_tag="VHR42_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025635.1"
                     /translation="MELQRAKLENIKTRLNHGEEVKSTRVESDRFCVQLTNGPEITVD
                     DNYKKQVENTINKVNRGVRENTPGPDKTLND"
     gene            complement(2047..>2281)
                     /locus_tag="VHR42_00175"
     CDS             complement(2047..>2281)
                     /locus_tag="VHR42_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX3025636.1"
                     /translation="AIFVILAVEVRFMNAGTIVWYNENQGFGFISPDGGGNKLYFNSS
                     QRDSLTVGQEVTFDIKINPNNKRQIAVNVVPKA"
BASE COUNT          613 a          476 c          561 g          631 t
ORIGIN      
        1 tatacatact ttgtaaaacc ggagcagtca aacccgccgg gcgcagcgcc acccatgacg
       61 taccgggttc ccatgaattg ctccgcatag gccacaatgc ctcccgcttc ctgcgatccc
      121 gaggcggaga gagcctgtgc accgccggag tcatcaccgc cgctgcaccc ggcgggtttg
      181 actgctccgg ttttacaaag tatgtataca gtaaatacgg cgtgtcactg gctcattccg
      241 cgctgatcca gtcatatcag gggagatcgg tcggaagaag cggacttcag gccggcgatc
      301 tggtgttctt tgacactaac ggcgggggag gctcgatcaa ccacgtcggc atttatgtcg
      361 gaggcggcag gtttgttcag gcgtgctcca gaaacccgaa tgcgatcacc gtcagcagtc
      421 tgaatgagcg ttattatcaa agaacttatg tgaccgcgcg tcgcattttg aattaaaatt
      481 cgtttggtta taaaaaaagt caccggtttt ccggtgactt ttttgctttc acggcgcggg
      541 taaagtgcga ttgttaaacc gggccgatca cgcggcattt ggcggacaca tggggtgggg
      601 ctccggatta tttcagtttg tcgcattctt tcatagttct ctgtaatggc cgcccgtgat
      661 agcgggtgat tcttttcaga ccgtataatt ctgcagggtt tttggtgatc ttgttgattc
      721 cgtagctgca ggagaaggta gcctgaaagc caaggctttt aataaccggg agagcttctt
      781 tgctgatact tccgtaaggg taggcaaaag tggtgggcag ctggccggta ttcgaagaaa
      841 taaggttctg gcatttcgac aggtcgtttc ggagcagatc ctgatactgg ccgacggatt
      901 cagattttga tttttcacag ccaaaccgtt tgcgtgtgat ggaatggaga ttataagtgt
      961 gattttgaat ttcgataaaa cccgaatcgg acatttcgtt cagctgtttc catgtgacat
     1021 gggcgtaatc gaggttgctg tcactgacgt tggaaaaatc ctcggtattt ttcccgataa
     1081 cggaaagaac aatcttgacc ccgtattttt taattaaggg gtaggcgtaa acgtaatcgt
     1141 tcagatagcc gtcgtcaaaa gtaatcatca cgggatgatc cggaaggctt ccctttccgt
     1201 tgacgtagcc gatcaggtct gacatgttga tggccgtata tccgcttttt ttaaggaatt
     1261 tcaaatcact ctcaaaatcc cagggtgtga tgctcatatt gttaagatgg gagggcctga
     1321 tttcatgata aagaatgatg ggaatccgga tgggggcatc tgttgaggtt tcagccgccg
     1381 tgaccggatg ctttccgata ttcagcagag agggcgatag gaataagata aacaaaatta
     1441 aaaaaataac ggttataatt ttataattca gtttaatgac cattccaatg cttccttttc
     1501 attgttgata attgaagcgg acaggagctt ttttcggtat gtgtgctctt ctgccgttac
     1561 atcgaccgtt ttcggagtca gtatccatta tattcaacaa aggcaatgac cattcttaat
     1621 aatatattct atggattata gcaatcgttt aaagtacagc gcatactttc gggcaggatc
     1681 ttttatgatg actttctccg ggtataataa ttacaggagg gatttttatg gaattgcaaa
     1741 gagcaaaact tgaaaatata aaaaccagat tgaatcatgg agaagaggtc aaaagcacac
     1801 gagtagaaag cgaccggttt tgtgtgcagc ttacgaacgg cccggagatt accgttgacg
     1861 acaattataa gaaacaggtt gaaaatacca taaataaagt aaataggggc gtccgtgaaa
     1921 atacgccggg ccctgataaa acgctgaatg attaaataaa acagcagcgc cttgcacagg
     1981 gtgctattag gatgatgatt ctggggttta taaaaagaag ccatctcgta cgagatggct
     2041 ttcaaattat gccttaggta caacgttgac tgcgatttgc ctcttgttat tgggatttat
     2101 ctttatgtca aaagttacct cttgcccaac tgtcaaagaa tctctctgtg atgagttgaa
     2161 atacagcttg tttccgccac catcaggaga tataaagcca aaaccctgat tttcgttgta
     2221 ccacacaatg gtaccagcat tcataaaacg cacctccacc gccagtataa caaatatcgc
     2281 a
//
LOCUS       DATALY010000008         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_779634,
            whole genome shotgun sequence.
ACCESSION   DATALY010000008 DATALY010000000
VERSION     DATALY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779634"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VHR42_00180"
     CDS             <1..233
                     /locus_tag="VHR42_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEX3025637.1"
                     /translation="VKDKELRVYNEIPNPKVVEAVGICPISGGVFRDSYAVANQLDAY
                     IPVDVNVPGCPPRPQAIIDGIAKAIEIWKTRF"
     gene            288..824
                     /locus_tag="VHR42_00185"
     CDS             288..824
                     /locus_tag="VHR42_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3025638.1"
                     /translation="MSTFYKVILRNIIKGPSTMKYPFEDSPASEKLRGKIKHNPEACI
                     ACHMCEYVCAGGAIRIQESEDKESFRYTVWHNTCTFCGLCEHYCPTNAIHLTNDYHTA
                     HLQEDKYDYCEKTIVKKQHCICCGEPIVTLSPMLIEKAYGKDGDVRGLSKMCPKCRRK
                     VLWEDGGLKNDRTCKSPE"
     gene            796..981
                     /locus_tag="VHR42_00190"
     CDS             796..981
                     /locus_tag="VHR42_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025639.1"
                     /translation="MTEPVKVPNKNRQCSQCGEPILPLPPMMVEKIYGKDGDVRGLSK
                     MCPKCRKKVLWKGGVTR"
     gene            978..1550
                     /locus_tag="VHR42_00195"
     CDS             978..1550
                     /locus_tag="VHR42_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX3025640.1"
                     /translation="MNEEVRNRFSSRLDEVLGKNFELKWDIDKKGVVCGWCRLVNPED
                     IYPAAELAAEFKGRVMTISALNTPIDFEGKLPLKEANPEAPINLVINYHLYFQGINCT
                     FTITLPDKKRSVKSITPVLKSADWHEREMQDLFNIKLEGHPNPKRLFLDESIHMDDHT
                     MIPLSEAMNEASTNTLWEKLMESGRKDEER"
     gene            1547..>2260
                     /locus_tag="VHR42_00200"
     CDS             1547..>2260
                     /locus_tag="VHR42_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX3025641.1"
                     /translation="MSSYNIPIGPIHVVLEEPIYFKLKVEGETVVGLDINAGHVHRGM
                     EFLAMHRNYYQNLTLTERVCSLCSNNHPFTYCMAVEKIAGIEISERAEYLRTIADEVK
                     RIASHLFNIGLIPHIIGFDSLFMHAMELRETMQDLKESVFGNRMNLSVNSIGGVRYDI
                     SDDQAKYILKTLDNLKKPFEEMVKIYQSNASIRRRTEGIGVLSYEKAVEYGVIGPVAR
                     ASGIPYDVRVDNPYAAYDKV"
BASE COUNT          663 a          515 c          575 g          507 t
ORIGIN      
        1 atgtaaaaga taaggagttg cgtgtctaca atgaaatccc aaacccgaag gttgttgagg
       61 cggtgggcat ttgccccatt tcgggcggtg tgttccgtga cagctacgcc gttgcaaacc
      121 agctggacgc atacattccg gtggatgtaa atgttcccgg ctgccccccg cggccgcagg
      181 caattattga tggcatcgca aaggcaatcg aaatctggaa aacaagattc tgaggggaaa
      241 agttttgtcc ttcccgcaaa aagcaccgcg aaaggatggt cataaagttg agtacgtttt
      301 ataaggttat tctgcggaat attataaaag gtccgtctac gatgaaatac ccctttgaag
      361 attcgcctgc gtcggaaaaa ctcaggggta aaatcaaaca caatccggag gcctgcatcg
      421 catgtcatat gtgtgaatat gtttgcgcgg gcggcgccat aagaatacag gaatcggaag
      481 acaaagaaag ttttcgctac accgtgtggc ataatacgtg cacattttgc ggcttatgcg
      541 aacactactg ccccaccaac gcaatccatt tgacgaacga ttaccacacc gcgcatttgc
      601 aggaggataa gtatgactat tgtgaaaaga ctattgtaaa aaaacaacat tgcatttgct
      661 gcggtgaacc gattgtaacg ctttctccca tgctgattga aaaagcatat ggcaaagacg
      721 gcgacgtgag gggcctgagc aagatgtgcc cgaaatgccg gagaaaagtt ttgtgggaag
      781 acggtggatt gaaaaatgac agaacctgta aaagtcccga ataagaaccg gcagtgctcc
      841 caatgcgggg aacccattct gccgcttccg ccgatgatgg ttgagaaaat atacggcaaa
      901 gacggcgatg tgagaggcct gagcaagatg tgcccgaaat gccgcaaaaa agttttatgg
      961 aaaggtggtg tgaccagatg aatgaagagg taagaaaccg tttcagcagc agactggatg
     1021 aggtgttggg caaaaacttc gaactgaaat gggacattga caaaaaaggt gttgtttgcg
     1081 gttggtgcag gctggtcaac ccggaagata tttaccccgc agcggaactg gcagccgaat
     1141 tcaagggcag agtcatgacc atatccgcgt tgaatacgcc gattgatttt gaaggaaagc
     1201 tgccgctgaa agaagccaat ccggaagccc ccatcaatct ggtaatcaat tatcacctct
     1261 attttcaggg aatcaactgc acctttacca ttacgctgcc ggacaaaaaa cgctcggtaa
     1321 aatcaatcac cccggtgctg aaaagcgcag actggcatga gagagaaatg caggaccttt
     1381 tcaacatcaa actggaaggc cacccgaatc caaaacggct gttcctggat gaaagcattc
     1441 atatggatga tcatacgatg atccctcttt cggaagcaat gaacgaagcg agcaccaaca
     1501 cgctatggga aaaactcatg gaatccggca gaaaggatga ggaacggtga gcagttataa
     1561 tataccgatc ggcccgattc atgtcgtgct ggaagaaccc atctatttca agctgaaggt
     1621 tgagggtgaa acggtcgtcg ggcttgatat caatgcaggc catgtgcacc gcggcatgga
     1681 attccttgca atgcaccgga actattacca gaacctcacg cttaccgagc gcgtttgttc
     1741 cctttgttcc aataaccatc cctttaccta ttgcatggcg gttgaaaaaa tcgcagggat
     1801 tgaaatatcg gaaagagccg agtatttaag aacgattgcc gacgaagtca aacgcattgc
     1861 atcccatctt ttcaacatcg gcctcattcc gcatattatc ggcttcgatt ctcttttcat
     1921 gcatgccatg gaactcaggg aaaccatgca ggatctgaaa gaatccgtct tcggcaaccg
     1981 gatgaacctg tccgtcaaca gcatcggcgg cgtccgctac gatatttcgg acgaccaggc
     2041 aaagtatatt ctcaaaactc tggataatct gaaaaagccg tttgaagaaa tggtgaaaat
     2101 ctatcagtca aacgcatcca tccgcagacg caccgagggc attggcgttc tttcctatga
     2161 aaaggctgtt gaatacggcg tcattgggcc ggtggcaaga gcttccggga ttccgtatga
     2221 tgtccgggtg gacaatccct atgcggctta tgacaaagtc
//
LOCUS       DATALY010000009         7390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_434195,
            whole genome shotgun sequence.
ACCESSION   DATALY010000009 DATALY010000000
VERSION     DATALY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434195"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..244)
                     /locus_tag="VHR42_00205"
     CDS             complement(35..244)
                     /locus_tag="VHR42_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025642.1"
                     /translation="MNRKRTAAIILNYNDSLNTLRLISQFKHYGVIDKILVVDNSDRD
                     GLDAGLPDFDGEKNHSDYESKQRLR"
     gene            complement(247..1113)
                     /gene="rfbA"
                     /locus_tag="VHR42_00210"
     CDS             complement(247..1113)
                     /gene="rfbA"
                     /locus_tag="VHR42_00210"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016136075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX3025643.1"
                     /translation="MKGIILAGGAGTRLHPITKAVSKQLLPVYDKPLIYYPLSVVMLA
                     GIREVLIISTPRDLPMYQDLLGDGSDFGIELSYAVQKEPKGLAEAFIIGEKFIGTDSV
                     CRALGDNFFYGQGLTQILHKASAIKNGAVIFGCYVKNPSAFGVVEFDASGSVLSSEEK
                     PKEPKSNYAVPGLYFYDNRVIEIAKNVKPSARGEVEITSVNNAYLNEGSLKVELFGRG
                     LAWLDTGTCQGLLQAANFVEAVQTRQGFYIACLEEIAFRQGFINRERLMERAKALSKT
                     DYGKYLEEIAGE"
     gene            complement(1300..2340)
                     /locus_tag="VHR42_00215"
     CDS             complement(1300..2340)
                     /locus_tag="VHR42_00215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3025644.1"
                     /translation="MKLLTIAVPAYNVEKTLSDTLESFCIDELLESLDVIIVDDGSTD
                     KTREIAKRFINSHPDSFRIISKKNGGHGSAVNTGIQNAAGRYFKVVDGDDRLEEDGFR
                     ALLKHLETTDSDLVATHYKKVPVDGGNPVPMRFESVLFNRQYSFGGLPADGSVYLGIH
                     AVTYKTALLKDTGIRLQEHTFYVDVEYGLLPMPFVKTVEFLDVYTYLYFVGSAGQSIN
                     YDNFVKRYDHHYRVVKRMVKFAATPGLDEAVTDYVYSVLDKLCFTHYMLSAFYDRDVK
                     RGKKRAAEFDAWLSASSPRLYRELGKSKYIRFLRATRFTVLPRGKALKKAVKSVSGSL
                     KQLFGRKGRFTY"
     gene            2550..3752
                     /locus_tag="VHR42_00220"
     CDS             2550..3752
                     /locus_tag="VHR42_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025645.1"
                     /translation="MNGLVISWFYPPATSAEGLVTFKLLKNSEFHYDVISAESRQWSY
                     DTDSPLSSPNITVHPVKSDHFSTFIREADETAGRLMGQNHYDFLMTRAMPAQSHTVGL
                     KIKKRSGVFWIASMADPIGRNPYDYDRYFGSAGKILKNPIRTAARVVIYLRNILFENK
                     VIRKADRIVFPSLNQCKYTLGKNYEKYKEKIMIIPHTFDPELCPPERTDAAANERITI
                     SHLGHLNGQRSAEGLISALSKLKRDKPELGRRLFFRLIGNIPKEQKELVEREGLTAMI
                     SVEKAVDYFESLRIMQESDILLLIDAKFNCMKENIFFASKLADYMGAGRPIIGLTDET
                     GPSGGIIKRSGGTVCNPEDIDHIYHLFRRIAEDGIPSHLPEVYRCYESRLSARKFDED
                     LKTCLHNK"
     gene            complement(3809..4036)
                     /locus_tag="VHR42_00225"
     CDS             complement(3809..4036)
                     /locus_tag="VHR42_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HEX3025646.1"
                     /translation="MANTNSSNEALIPQAREALDRFKMEAASEVGVNLKQGYNGDLTS
                     RQAGSIGGQMVKKMIAQVENSMAGGSTSGMR"
     gene            complement(4426..4992)
                     /locus_tag="VHR42_00230"
     CDS             complement(4426..4992)
                     /locus_tag="VHR42_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025647.1"
                     /translation="MNTVLLYGSEKNARTGIALFAAIRAVGCSALHVTPKSISMIPPG
                     NRAPDFLILEFTEIPELIVGNGLVLFEQESGLSLFQKQPEIPVGFTAVVDPDNADAIE
                     MLQRNSMQTVTCGMSQKDTVTFSSLDSDRAVVSLQRELRSLSGTTIEPREFPVGFSKQ
                     PGEYQLLASAAVLLLSDIPIPDDGFMLS"
     gene            5521..5793
                     /locus_tag="VHR42_00235"
     CDS             5521..5793
                     /locus_tag="VHR42_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025648.1"
                     /translation="MQNQKSEKKEQSDTLLEKRLNELEREYDSKRDPVNDLRLEELNF
                     YLSGLLGRNSLHYLREYADLLIARYNTDAGWFYNKGYEDAFKSMSR"
     gene            complement(6139..6936)
                     /locus_tag="VHR42_00240"
     CDS             complement(6139..6936)
                     /locus_tag="VHR42_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3025649.1"
                     /translation="MKVLILNKKSILKGILILCLAVAVLIAGISFVPRVVATMTGNRE
                     LPIYKVDRQEKIASLSFDAAWGNEDTNQLIKILNQYHVKATFFVVGQWVDKYPESVKA
                     LSDAGNEVMNHSNTHPHMTKLSRDDMLKEINACDEKISKITGKKPILFRAPYGDYSNA
                     LIETLTQSKHYCIQWDVDSLDWKEIPAAKIQQRVLSKVKNGSIILFHNAAKYTPEALP
                     GILKSLQDQGYKLVPVSQLIYQGSYTIDHAGTQHTSEAAQSSQGASS"
     gene            complement(7037..>7390)
                     /locus_tag="VHR42_00245"
     CDS             complement(7037..>7390)
                     /locus_tag="VHR42_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3025650.1"
                     /translation="AGISFVPRVVATMTGNRELATIKVLGFYDRELAVYLYRENAVLT
                     ILGTGLGIWLGYYMERYVTLTSEVDLVMFSRDILWPSYVLSALLTLLFAAFVNLVMFR
                     RLKNINMVSSLKSGE"
BASE COUNT         1934 a         1837 c         1703 g         1916 t
ORIGIN      
        1 ctcattgaga tagtcaagcc ccgcgtaaat tccctcagcg taaccgttgt tttgattcgt
       61 aatcagaatg atttttttcc ccgtcaaaat cgggcaggcc ggcatccaga ccgtcccggt
      121 ccgaattgtc gacaacgaga attttatcaa ttacgccata gtgtttaaac tgggaaataa
      181 gcctcaacgt attcaggctg tcattataat tcagaatgat ggccgcggtt cttttcctgt
      241 tcaatcctat tctcccgcaa tttcctcaag atacttgcca tagtccgttt tggaaagcgc
      301 tttagccctt tccatcagtc gttcgcggtt gataaagccc tgtctgaaag caatttcctc
      361 caggcacgca atgtagaagc cctgtctggt ctggacggct tccacaaaat tggcagcttg
      421 cagaaggccc tggcacgttc ccgtgtcaag ccacgcgaga ccgcggccaa aaagctcgac
      481 tttcaggctt ccttcgttca gataagcgtt attaacgctc gttatttcga cttccccgcg
      541 cgcggaaggc ttcacgtttt tggcaatttc gatcacgcgg ttgtcataaa aatacaagcc
      601 cggaaccgcg taattcgatt tcggctcttt cggcttttcc tcactcgaca aaacactgcc
      661 gctcgcgtcg aactcaacca cgccgaacgc agacggattt tttacataac aaccgaaaat
      721 aaccgctccg tttttgattg cagacgcttt gtgcaatatc tgcgtcagac cctgaccgta
      781 aaagaagtta tcgccaaggg cacggcagac gctgtcggtc ccgataaatt tctcaccgat
      841 gataaaggcc tccgcaaggc ccttcggctc tttctggacg gcgtaggaga gttcgattcc
      901 gaaatcactg ccgtcgccga gaagatcctg atacatggga agatcccttg gagttgatat
      961 aatcagcact tcccggatcc ccgcgagcat cacaactgaa agaggataat aaatgagagg
     1021 cttgtcataa accggcaaaa gctgttttga cactgccttg gtaatcggat gaagtcttgt
     1081 gccggctcct ccggcaagaa taataccttt cataattcct gtcccgccgc tgccgcaacg
     1141 gcccataaat gtcagcttcc cgcggcaaaa tttgggaaga gcatattttc ctatcgaaat
     1201 ttcattgaac tcttacatta cagatcattc cattaagcaa tatcgaaagg gcggatgccg
     1261 ccggtctccc tcccgttatc cggaagaaaa atcagcgtgt caataagtaa atcgtccctt
     1321 tctcccaaaa agctgtttca gcgacccgct tacgcttttg accgccttct ttagcgcctt
     1381 tcctcttggc agaacggtga atcgggttgc ccttaaaaaa cggatatatt tactttttcc
     1441 aagctcgcgg taaagccggg gagaactggc agaaagccat gcgtcaaatt cagccgcgcg
     1501 ctttttcccg cgttttacat cccggtcgta aaaggccgag agcatataat gggtaaaaca
     1561 gagcttgtcc aacaccgaat aaacgtaatc cgtcaccgct tcatccaggc ccggagtcgc
     1621 cgcgaatttt accattcgtt tgacaacgcg gtaatgatga tcataacgtt ttacgaaatt
     1681 atcataattt atactctgac cggcagagcc cacaaagtaa aggtaagtat aaacatccag
     1741 aaactcaacg gtttttacaa aaggcatcgg aagcaaaccg tattcgacgt caacataaaa
     1801 ggtatgttcc tgaagccgga ttccagtgtc tttgagcagg gcggttttat aggtcaccgc
     1861 atggatgccg agatacacac tgccgtcggc cggcaggccg ccaaatgaat actgcctgtt
     1921 aaacagcacg ctttcaaacc gcatcgggac ggggtttcct ccatcgaccg gtactttttt
     1981 ataatgggtg gcgacaagat ccgaatcggt ggtttccaaa tgcttcaaaa gggcccgaaa
     2041 gccatcctcc tcgagcctgt catctccatc aacaacttta aagtatctgc ccgcggcatt
     2101 ctggattccg gtgttgaccg cagacccgtg ccctccgttc tttttggaaa tgatccgaaa
     2161 agaatcgggg tggctgttga tgaaccgttt tgcgatttcc cgtgttttat ctgtcgaacc
     2221 gtcgtcaaca ataatgacat ccaacgattc aagtaattca tcaatacaaa aagattcaag
     2281 tgtgtcgctc agggtttttt ccacattata agccggcacc gcaattgtta acagtttcaa
     2341 aatatgcctc cgtttcaaac gcctcgtttt ctttcccttg gaaagacgcc gttattccgt
     2401 tgttggctgg tgcggtggaa tgcggtcgcc gggggacccc cgaaccgtta ccgtatgaaa
     2461 ggaattattg gcaaaaactc tttaatttaa cgtcaatcta ttgtatcatt aatatacgtc
     2521 aatttcaaga cccaacggag gcttaaggta tgaacggttt ggtcatctcc tggttttacc
     2581 cccctgcgac atccgcggaa ggtttggtga ccttcaagct gcttaaaaat tcagagtttc
     2641 attacgacgt catcagcgcc gagagcaggc aatggagtta cgatacggat tcaccgctgt
     2701 caagcccgaa catcacggtt catccggtga aatcggatca tttttcaact tttatccggg
     2761 aagcggatga aacggcaggc cggctgatgg gtcaaaacca ttacgacttt cttatgacgc
     2821 gcgccatgcc ggcgcaaagc cacacggttg ggcttaaaat caaaaagaga tcaggcgttt
     2881 tctggatcgc gtccatggcc gaccccatcg gccgaaaccc ttatgattac gaccgttatt
     2941 tcggcagcgc cgggaaaata ctgaaaaacc cgatacggac ggcggccagg gttgtgatct
     3001 atttgagaaa tattctgttc gaaaataaag tcattcgcaa agcggatcga attgtctttc
     3061 cgagtttgaa ccagtgcaaa tatactctgg ggaaaaatta tgagaaatac aaagaaaaaa
     3121 tcatgattat cccccatacg tttgacccgg aactatgtcc gcctgaaagg acagatgcgg
     3181 cagcgaatga aagaattacc atatcccatc tcgggcatct gaacggccag cgctcggcgg
     3241 aaggattgat ttcggcgctt tcaaaattaa aaagggacaa gccggagcta ggccgcaggt
     3301 tatttttcag gttaatcgga aacattccga aagagcagaa agagcttgtt gaaagggaag
     3361 gcctcaccgc gatgatcagc gtcgaaaaag ccgtcgatta ttttgaaagc ctgagaatca
     3421 tgcaggaatc ggatatcctt ctgctgattg acgcaaaatt taactgcatg aaagaaaaca
     3481 ttttttttgc ttctaagctg gcggattata tgggcgcggg cagaccaatc atcggcctga
     3541 cggatgaaac cggcccgagc ggcggaatca ttaaacgatc gggaggaacg gtatgcaacc
     3601 cggaagatat tgaccatatt tatcatcttt tccgcagaat cgcggaagat ggaattccgt
     3661 cccacctgcc tgaagtctac cgctgctatg aatcccggct ttctgcgcgg aagtttgacg
     3721 aagatctcaa aacctgtctc cataacaaat aagtaaagaa aatattaaaa ggggttccca
     3781 tacgggaacc ccttttatca gcggtttgtt atctcattcc gcttgtggaa ccgccggcca
     3841 tgctgttctc aacctgagcg atcatcttct tgaccatctg tccgccgata gaaccggcct
     3901 gccttgaggt aaggtcaccg ttgtaaccct gtttaaggtt tacgccaacc tcgctggcag
     3961 cttccatctt aaaacgatcc agagcttcac gagcctgagg aatcagtgct tcattgctac
     4021 tattagtgtt agccatatta tatctctcct tagaaaaagg tattttaatg cgtttgcatt
     4081 aatattctgt tctttcgcgg gtgaattatt aaaagtaatt tttagcaaca ttgttattgt
     4141 ttagcaacgg tttttatccc aaaaaacttc cgaccttctg aacgccggcg gctgcctgcc
     4201 attgggtggt gaaacattgg tcttaacggg aattcatccg tcgttttgag ctgctcaatt
     4261 gataattgat ttatttaaaa cagctcctga tcccgtttgt gatcggatca cgctgatggg
     4321 tatgaattta aatggtttca cctgcactgt ttttcacggc gaaaatctct ggaggtctat
     4381 tttccgtctg ctattctcga ttcccgcccc gctcctttgc gacagctacg agagcataaa
     4441 tccgtcatcc ggaatcggga tatccgataa caacaaaacc gccgcagacg ccagcagctg
     4501 atattcgccc ggctgttttg aaaaaccgac cggaaattcg cggggctcga ttgtggttcc
     4561 gctcaaagac ctcagctccc tttgaaggct gacgaccgcc ctgtctgaat caaggctgga
     4621 aaaagtcacg gtatctttct gcgacatacc gcaggttacc gtctgcattg aattgcgttg
     4681 aagcatttct atggcgtcgg cattgtcggg gtcgaccacc gcggtgaacc ccaccggaat
     4741 ttccggctgt ttctgaaaca gagaaagacc actttcctgc tcaaacagca caaggccgtt
     4801 cccaacaatc agctcgggta tttcggtaaa ttccagaatc agaaaatccg gcgccctgtt
     4861 tccgggggga atcatagaga tggattttgg cgtgacatga agtgccgagc agcccaccgc
     4921 ccggatggcg gcaaaaagtg cgattcctgt tcgggcgttt ttttcggaac catagagcaa
     4981 taccgtattc atgaatcggc ccccatctat tcgattgatt ttttcctgtc tttattttct
     5041 gatcctttcc tcaaaatatt tatcgggaat aaaattttaa gattcgatca tgaacaaagg
     5101 ggagcttcat gccgcaaatc cgcacgaagt tcccctttta aatttcgacc atatttgtaa
     5161 atcaaaggca attatatctt gcataacggg ttttgctggt ttataatgga catatacgga
     5221 acagcggccc gaaagaaagg gccgaagcag cctgaatgtg ttcaaagcac aatcaggcct
     5281 gtgcaggtgt cgagaccacc tacataaccg gcttttgccg gcgctccgac gttatttatt
     5341 ataaacgatt caccgtccca ttgcaagcaa ggtgaggccg cgctcagcgc tcccctcttc
     5401 tttgtcttgt ctgttggtct ggcggttttg ggtttgtatg tcgagcgggt gtaggtatgt
     5461 aatacctgcg cccgcttttt cgggccccga tccgtcaaat caacagagga gcgaggatct
     5521 atgcaaaatc aaaagagcga aaaaaaggaa caaagcgaca cgcttctgga aaagaggctg
     5581 aacgagctgg aacgcgaata tgattccaaa cgcgacccgg taaacgactt aaggcttgaa
     5641 gagctgaatt tctatttatc cggtctgctg ggccgaaaca gcctccacta cctgcgggaa
     5701 tacgccgacc tgctgatcgc gaggtataac accgacgccg ggtggtttta taacaaaggc
     5761 tatgaagacg ccttcaagtc gatgagccgc tgatgaccgg cggggatatt gacctcgatt
     5821 aatcgtgaaa aacaccgcct gttatttcgg ctgtattcgc aaatattgtg ggtataacaa
     5881 tatgatttcg ggtaactaaa gactgtatgt ctttattgca tctacggttt tatccaccag
     5941 caaatcaaca ttattttatg ttccaaatta gtataacata tacaccggat acccacaaaa
     6001 ctgaaaacag cgccattacc tttagaaact ccttttatca atcggcccgc tgcgccggtt
     6061 gaatcgctat ttgacgcgtc caaaaagccg ccggtattca taccggcggc ttaaaactga
     6121 ttgaacctga atgcggtttc aggagcttgc cccctggctt gactgtgcgg cttcggaagt
     6181 atgctgtgtt cccgcgtgat caatcgtata agagccttga taaatcagct gcgaaacggg
     6241 cacgagttta tagccctgat cctgcaggct tttaagaata cccggcaggg cctcgggagt
     6301 atatttcgcg gcgttatgga acaggatgat cgacccgttc ttcactttgg agagaaccct
     6361 ctgctgtatt ttagcagccg ggatttcctt ccagtcaagg ctgtcgacat cccactggat
     6421 gcagtaatgt ttcgactggg taagagtttc aatcagcgca ttgctgtaat ccccgtaggg
     6481 ggcgcggaac agaatcggct ttttgccggt gatcttgctg attttttcat cgcaggcatt
     6541 gatttccttg agcatgtcgt ctctcgataa tttggtcata tgaggatggg tgttggaatg
     6601 gttcatcact tcatttccgg cgtccgacaa agctttcacc gactccggat atttatcgac
     6661 ccactggccc accacaaaaa aggttgcctt gacatgatac tgattcagta ttttaatcag
     6721 ctggttggtg tcttcgttgc cccaagcagc atcaaaagag aggctggcga tcttctcctg
     6781 ccggtcgacc ttatagatgg gaagctcccg gtttcccgtc atggtggcta ccacgcgggg
     6841 aacaaaactg attccggcta tcagaacggc caccgcaaga cacaagatta agatgccctt
     6901 taagattgat tttttattca gaataagaac tttcatgata actactcctg tccgttaacg
     6961 ttcagtataa gcctatgcac agacaggttg gactaatgcg taaagcgcaa aaattatcct
     7021 caaagtccgg cggttttcac tcgccgcttt tgagggaaga aaccatattg atatttttca
     7081 ggcgacggaa cataaccaga ttgacaaacg cggcaaacaa aagggtgagc agcgctgata
     7141 acacatagct cggccaaaga atatcgcggc tgaacatcac aaggtcaacc tccgaggtga
     7201 gagtaacata acgttccata tagtaaccca gccagatccc gagacctgtt cccaaaatgg
     7261 tcaggacggc attctcacga taaagataaa ccgcgagttc cctgtcataa aagccgagca
     7321 ctttgatcgt cgcaagctcc cggtttcccg tcatggtggc taccacgcgg ggaacaaaac
     7381 tgattccggc
//
LOCUS       DATALY010000010        10341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_348147,
            whole genome shotgun sequence.
ACCESSION   DATALY010000010 DATALY010000000
VERSION     DATALY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348147"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VHR42_00250"
     CDS             <1..317
                     /locus_tag="VHR42_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025651.1"
                     /translation="AASIFSATGAVACFEQTAAEILNQFEPVYQSFLTGGAETFLNDY
                     RKNCVTLGHMVKVSGGGRELEGLALDVFPDGRLAVKTETGVEKVEAGDVSVRGIMGYS
                     DL"
     gene            449..1543
                     /gene="ychF"
                     /locus_tag="VHR42_00255"
     CDS             449..1543
                     /gene="ychF"
                     /locus_tag="VHR42_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX3025652.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITNAGAESANYPFCTIEPNVGVVAV
                     PDKRLDVLADLYHPVKITPAVIEFVDIAGLVRGASKGEGLGNKFLSHIREVDAIIHVT
                     RCFEDTDVIHVEGSVDPLRDIETINLELIFSDMEILDRRIDKAKKMLKGDKKYQDEID
                     LFERIKTTLEAGLPARAVECTPKEEAILSTVSLLTEKPVIYAANMSEKDFKGNYDTKT
                     NYQKVKELAEKEHAEVIPICAKIEEELSGLESDDRELFLADLGLKNSGLDRLIQASYR
                     LLGLISYLTAGQPEVRAWTITKGTKAPQAAGKIHSDFERGFIRAEVVAYEDLIACGSI
                     AAAKEKGLVRSEGKEYVMQDGDVVLFRFNV"
     gene            complement(1708..2818)
                     /locus_tag="VHR42_00260"
                     /pseudo
     CDS             complement(1708..2818)
                     /locus_tag="VHR42_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(2968..4140)
                     /locus_tag="VHR42_00265"
     CDS             complement(2968..4140)
                     /locus_tag="VHR42_00265"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HEX3025653.1"
                     /translation="MNEMQLKYGCNPNQTPARIFMKSGELPIKVLNGRPGYINLLDAL
                     NGWQLVQELKKSTGLPAAASFKHVSPAGAAVGTPISETIQKICFADDLELSPLASAYA
                     RARGADRMSSYGDFIALSDICDVPTARLIAREVSDGIIAPGYDEQALAILKSKRKGNY
                     TIIRMEETYRPGLIEQKDVFGITFEQGRNEVPISEALLQNIPTQNKEIPQNAKIDLII
                     SLITLKYTQSNSVCYVKDGQAIGIGAGQQSRVHCTRLAGDKADKWYLRQNPKVLSLPF
                     QESIRRPDRDNAIDVYLSDDYGDVLSDGCWEELFTKKPEPLTREERKEWIKTLKGVSV
                     GSDAFFPFGDNIERAHRSGAEYIAQTGGSIRDDNVIETCDKYHIAMAFTGVRLFHH"
     regulatory      complement(4183..4259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(4434..6095)
                     /locus_tag="VHR42_00270"
     CDS             complement(4434..6095)
                     /locus_tag="VHR42_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025654.1"
                     /translation="MKKLRLISLVILFILFGIIIFVASPNLNPLYDDGLAFWGLVITT
                     FVVVYWLISGVEKVKGFAGEIKDRATGKSSKITKLPKMGAALVVIALAPWVILIVVNI
                     VSSVVFHSSRYRDQLTKPESRQFTSDLQAIDVSQLPVVDYNLAYKLADKKLGEKPSLG
                     SQVTLGDPTIQKVDGKLVWVVPLEHSGFFKWLSNMEGTPGYIVVSATDPSDVTYKDSY
                     PIKYQPSAYLLDNLTRHVRFSGGLFTGITDYSFELDDSGKPYWVVTTYKNELGFNLPE
                     ANGVLVVDAATGATKHYSIKDVPSWVDRVQPGDYIMTQIKNRGKYIHGIFNFSNKDKF
                     EPSDNYAIVYNNGRCYLFTDLTSVGSDQSATGFMMVDMVTKKTYLYQMSGATEYAAQK
                     SAEGRLQNYKYTASYPLITNVNGEPTYFMTLKDEEGLIKQYAFVSVKNYMIVGNGDTL
                     QDALDDYDGKVRAAPDGNSISAGAKKVTLDGTIARIAMEQESGNSIFKLMLTNKPGVI
                     FSAAYDLSNELALTRDGDKVRLVYSQTDKSLITLTGFTNESIQLK"
     gene            complement(6280..6504)
                     /locus_tag="VHR42_00275"
     CDS             complement(6280..6504)
                     /locus_tag="VHR42_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HEX3025655.1"
                     /translation="MSNSRKSRMLNPQAYDVMNQFKMEVASEMGRTQFVKENNDHYKG
                     DVTARENGSQGGPIGGEMVRRMIKMSEEHL"
     gene            6857..8410
                     /locus_tag="VHR42_00280"
     CDS             6857..8410
                     /locus_tag="VHR42_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX3025656.1"
                     /translation="MSILDVTGLTHYFGDKKIFSDTGLSLFRGDKMGLTGLNGAGKST
                     LINILAGTVIPDQGYIKWNPKVRAGYLDQQANIDGCQSVRDYLKGAFDHLYQTETLLE
                     ETNAKIAACKDRTELEKLMAVSGDCQNLLESNNFYGLESEIDKVAAGLGITAFGTDKP
                     VEKLSGGQRAKVMLAKLLLENPDVLLLDEPTNFLDREHIEWLGKFLINFKGSFLLVSH
                     DFPFLNRVVNCICNIEFFTITRYNGSFESFLKQKEQKAEEYQRRYSRQQKEIAKLEDY
                     IGRNLYRASTTAMAQSRRKKLEKMEKIDRPQQAVKPTFTFKYQSIPPKIMLRVKDLEV
                     GYGEPLLPKIAINLRTGERLSVSGFNGIGKTTFLKTIAGLLPKISGSFKYDPNVVIGY
                     YEQEHHWEDPSRTAFNQIRELFPKLKDKDVRGNLAKCGLKHEQAMQSLSTLSGGEQAK
                     VKLCILTLTPCNLLILDEPTNHLDQNAIEHLKQAIMDFPGSVIFVSHSKEFCEALADK
                     QLNMEKLFD"
     gene            8610..9206
                     /gene="yyaC"
                     /locus_tag="VHR42_00285"
     CDS             8610..9206
                     /gene="yyaC"
                     /locus_tag="VHR42_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HEX3025657.1"
                     /translation="MEHMVYYDSREKLVQWKLDETLYSLISKVDKSFRQICVICIGTD
                     RSTGDSFGPLTGHMLSRLTAFHFNLYGTFEAPVHALTLPETLDRINSKDSLVIAVDAS
                     VGNGEQVGYIGMGYEPVRPGSGLGKELPPVGDISITGIVAMGGLAPFIMLQNASLGMV
                     YHMAEKTFLAIQYSLYKLHQREIAPAENDRDLIRKPSA"
     gene            complement(9203..10066)
                     /gene="rsmI"
                     /locus_tag="VHR42_00290"
     CDS             complement(9203..10066)
                     /gene="rsmI"
                     /locus_tag="VHR42_00290"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX3025658.1"
                     /translation="MAQGQLVLVATPIGNLGDFSTRAEQALRDADFIAAEDTRVTIKL
                     LNRFEIKKPMISYFEHNKKLRGEEIVRRIEAGETCALVTDAGMPAISDPGEDLVALCA
                     DNGIKVTIVPGPCAVVSALAVSGLPTGRFSFEGFLSVNKKSRREHLQSVREDQRTLIF
                     YEAPHKLRTTLDDLLAGLGDRRIALVREMTKMHEEVVRTTISKAVEFYAQNTPKGEFV
                     LVVEGMIRQETEDGEENRIEQAAQLVRELVGEGMSHSEAVKTASARLGVRKSELYHTT
                     LGNENFQDIEG"
     gene            complement(10092..>10341)
                     /locus_tag="VHR42_00295"
     CDS             complement(10092..>10341)
                     /locus_tag="VHR42_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEX3025659.1"
                     /translation="CLCHRPGRLAEILSLMARYRVEPKRLRFVQQKAGSEPWLFLAEG
                     RKNASPGGLRVEPVLLFEEPDGSPGMELRRIYGAEFIK"
BASE COUNT         2576 a         2671 c         2434 g         2660 t
ORIGIN      
        1 acgcggcctc tatattttcc gccaccggcg ccgttgcctg ctttgaacag acggctgcgg
       61 aaattctaaa ccaatttgag ccggtttatc agtcctttct gaccggaggg gccgaaacgt
      121 ttctgaacga ctaccgaaaa aactgcgtta ccctcggcca tatggtcaaa gtgtcgggag
      181 gcgggcgaga gcttgaaggt ctggctctgg atgtttttcc cgatggcagg ctcgccgtca
      241 aaaccgaaac aggcgtcgaa aaggttgaag cgggggatgt ttccgtcagg ggaatcatgg
      301 gttattcgga cctgtgaatc ttccgaaaca gttgattgac actgcgcaat cggctataat
      361 gttgaaataa cactaaattt aatacatcag atccgggaat tcggaatttt ccgcctggtt
      421 ctggttcttt cggaaaggat agaattttat gaaactcgga attgtcggac tgccgaacgt
      481 gggcaaaagc acgcttttta acgcgatcac caacgccgga gccgaatcgg cgaactaccc
      541 tttttgtacc attgagccca atgtcggcgt cgtcgccgtt ccggataaga gactcgacgt
      601 tctggccgat ctttaccatc ccgtcaagat tactcccgcc gtaatcgaat ttgtcgatat
      661 cgccgggctt gtgcgcggag catccaaggg cgaagggctg gggaacaagt ttctctctca
      721 tattcgggaa gtggacgcca tcatccatgt gacccgctgt tttgaagata ccgacgtcat
      781 tcatgtcgag ggcagcgtcg accctctaag agacattgaa accattaatc tggaactgat
      841 tttttccgat atggaaattc tcgaccgcag aattgacaaa gccaaaaaaa tgctcaaagg
      901 cgataaaaaa tatcaggatg aaatcgacct gtttgaacgg atcaaaacaa cgctggaagc
      961 cggacttccg gcccgggccg tggaatgcac accgaaggaa gaagctattc tgtccaccgt
     1021 gtcgctgctg accgaaaagc ccgtcatcta cgccgccaac atgagcgaga aagattttaa
     1081 gggcaactat gacaccaaga ccaattatca gaaggttaag gagctggcgg aaaaggaaca
     1141 tgcggaagtg attccgatct gcgccaaaat cgaggaagag ctttccgggc tcgagtcgga
     1201 cgaccgggag ctgtttttag cggatctggg gctgaaaaac tccgggcttg accgtctgat
     1261 tcaggcaagc tatcggctgc tcggcctgat ttcctacctc acggcaggac agccggaggt
     1321 tcgcgcctgg accattacca aaggcaccaa agccccgcag gcggcaggca aaatccattc
     1381 cgattttgaa cggggattta tccgcgcgga agtggtagcc tatgaggacc taatcgcctg
     1441 cggcagcatc gcggccgcca aagaaaaagg ccttgtccgc agcgaaggca aggagtatgt
     1501 gatgcaggac ggcgacgtcg ttttgttccg gtttaatgtt taatcgtttg tgattaggaa
     1561 taacgtcctt aacaaaggaa tacagtgctt caatgagata gtaaaatcgg cctggtgttt
     1621 attataaaaa caccaggccg attttactta taaatagaac ctgtttgatg attgcttttt
     1681 catcaattat tgatttctaa aggcggctca aaccgccttt atgatttcca caacaaactc
     1741 ggttgctttg acaagatctt caatatcaat aaattcctca acggtgtggc ctctgtgcat
     1801 cgccgtgctg gtgtcaacag ctgtgattcc cctgtcattg agcacattga tatcgcttcc
     1861 gccgccggaa acctccaaga ccagattgac acccgctttt ggggccgctt ttttcataat
     1921 ggcgataagt tcatcgtttt cgctgacgct gaatcgcgga tattccttat agaattggaa
     1981 ttccacgctt cctccgaact tataggcgga ctgcaagaag cattgacgca tatgctcggt
     2041 ctgaaggtcc agtttttcat cgtttctgct gcgcgcctct gctttgattt caatcctgtc
     2101 gcatacgata tttgttgcct tacctccgtt gatgctgcca atattcgcag tggtctcctc
     2161 atcaaccaac ccgagacgca tatgtgagat ggcgtcggcc gcaatctgaa tggcgcttac
     2221 ccctttttgg ggagcgacgc cggcgtgggc cgccttgccg gtaattacga cgtcgatgat
     2281 gttttgggat ggccccccga tcccggtggc gcctatctgg ccgcccgcgt ccagcgcaac
     2341 ggcatacttt gcgtaaattt ttgaataatc cagatttttt ggcgccaagg agtccgactt
     2401 cttccgcaac tgtaaaagcg atctggatat caccgtgctc ggcaccgctt tcctgtatca
     2461 aacggatcgc ctcaaggatt gctgtgatcc cggaaacatc gtccgcgccc agaacagtcg
     2521 ttccgtcgga atggatcacg cttccgtcca taacggcctt tttgccgata ccgggcgtaa
     2581 ccgtatccat atgcgccgaa aacaggattg ccggaacatc cttggtaccc ttcagtgtgc
     2641 agatgagatt tcccgtgttg ccgccgattt ttttaccggc atcatcctcg taaacctcaa
     2701 tccccatgct cttgagctta ccgatgagtc tgtccgccac ttctctttcc tgacggctaa
     2761 ggctgtcgat ggcaaccagc tccaaaaaat cgttgaccag tctctctctg ttaaccattt
     2821 atgctccccc ttgattcaat gagtattttt gccatgttcc attacatcac aatttaattg
     2881 atttttcaag tgttgttaca gctgaacggc aaagccgatc aaaagttttg tgtttacaga
     2941 tcccctgctc tatccgatta caataagtta atggtgaaat aatcggacac cggtaaaggc
     3001 catcgcaata tgatatttat cacaggtttc aatgacgttg tcgtcgcgaa tcgagccgcc
     3061 ggtttgggcg atatattcgg cgccgctgcg atgcgccctt tcaatgttgt ctccaaacgg
     3121 gaagaaggca tcagaaccta ccgacacacc tttgagtgtt ttgatccatt cctttctttc
     3181 ttcccgggtt aaaggctcag gcttttttgt aaataattct tcccagcagc cgtcggagag
     3241 gacatcccca taatcatcgg aaagatacac gtcgatcgcg ttatcacggt ccggacgacg
     3301 gatgctttct tgaaacggca gactgagaac ctttggattt tggcgcagat accatttatc
     3361 cgctttatcg ccggcaagac gggtgcaatg gactcttgac tgctgcccgg cgccgattcc
     3421 aatcgcctgg ccatccttca catagcatac cgagtttgac tgcgtatatt tcagggttat
     3481 caacgaaatg atcaagtcga tttttgcatt ttggggaatt tctttatttt gagtcggaat
     3541 attttgaagc agcgcttcgg atattggaac ctcgtttctg ccctgctcga atgtaatgcc
     3601 aaaaacatcc ttttgctcaa tcagacccgg gcggtaggtc tcctccatcc ggataatcgt
     3661 ataattgcct tttcgctttg atttcagaat agcaagagcc tgttcatcat aaccgggcgc
     3721 gatgataccg tccgagactt cccttgcaat gagccttgct gtcggaacgt cacagatatc
     3781 ggataacgcg ataaaatcgc cataggagga cattctgtcg gcgcctctgg ctcttgcata
     3841 agccgaagcc agcggggaca gctccaaatc gtccgcaaaa caaattttct gaatggtttc
     3901 actgatcggc gtgcccacgg ctgcaccagc cgggctgaca tgcttaaacg aagcggcggc
     3961 cggcagcccg gttgactttt taagctcttg taccagttgc cagccgttca gcgcgtccaa
     4021 gagattgata tacccaggtc tgccgttcag aacctttatg ggcaattccc cgcttttcat
     4081 aaagatccgg gcaggtgttt gattcggatt gcatccgtac ttcaattgca tttcattcat
     4141 gacagatcgc tcctttttgc aaaataaaaa gccaatccac cggactttcg cccagacggt
     4201 cggctttttc tgtcatactc ccttgtggta ttccacatcc gtcagtcata tgactcatta
     4261 ctaataaatt acaccgaaaa tgcagttttg tcaatgcgga gctgaatctt cgaaaaacaa
     4321 aagcggtttg cttttctccg ctacacaacg ctttaaaata gaattcaaaa gtattgcacg
     4381 tgatgataca aaaaagcctg ccgtaataaa cggcagactt ttcaatgtta ttactatttt
     4441 aactgaatgg attcatttgt gaaaccggtc agcgtaatca gcgatttatc ggtctgtgaa
     4501 taaacaagcc tgactttatc cccgtcacgg gtcagcgcca gctcattgct cagatcataa
     4561 gccgcagaga aaatcacccc cggtttattt gtcagcatca gtttaaaaat cgaatttcca
     4621 ctttcctgct ccatcgcgat tctggcgatc gtgccgtcaa gcgttacttt ttttgctcct
     4681 gcgctgatac tgtttccatc cggtgccgct cttacctttc cgtcataatc gtcaagcgca
     4741 tcctgaagtg tgtcgccgtt tcctacaatc atataatttt tcacggaaac aaacgcatat
     4801 tgtttaatca ggccttcctc atccttaagc gtcatgaaat aggtcggctc gccgttgaca
     4861 ttggtaatca gcggatagga cgcggtatac ttatagttct gcagccgccc ctccgcggat
     4921 ttctgcgccg cgtattcggt ggctccgctc atttgataga gataagtttt ctttgtgacc
     4981 atatccacca tcataaatcc ggtagcgctc tgatccgatc cgacgctggt caggtcggta
     5041 aagagataac atctgccgtt attatacacg atggcgtagt tatcggatgg ctcaaactta
     5101 tccttattgg aaaaattaaa gattccgtga atatattttc ctctgttttt gatctgagtc
     5161 atgatataat cgcccggctg aacccggtca acccaggagg gaacatcctt gatggaataa
     5221 tgtttggttg cccctgttgc cgcatccacc acaagcactc cgttggcttc cggcagattg
     5281 aagcccagct catttttata agtagtcact acccagtaag gcttgccgga atcgtcaagt
     5341 tcgaaggaat aatcggtgat accggtaaag agcccgccgg aaaagcgcac atgtctggtc
     5401 agattatcca gaagataggc agaaggctga tatttgatcg ggtagctatc cttataagtg
     5461 acatcggaag ggtcggtcgc cgacacaaca atatagcccg gcgtgccttc catattggac
     5521 agccacttga aaaagccgga atgctccagc ggaacaaccc agaccagttt cccgtcgact
     5581 ttctggatcg tagggtcgcc cagcgttacc tggctgccaa gggaaggttt ttctcccagt
     5641 ttcttatccg caagcttata ggcaagattg taatccacca ccggcagctg ggatacgtca
     5701 atcgcctgca aatcggaagt aaactgcctg ctctcaggtt tggtcagctg atcccggtag
     5761 cgggaggagt ggaacaccac agacgatacg atattaacaa caatcagaat tacccacggc
     5821 gccagtgcga tcaccaccag ggcggcgccc atctttggca gtttcgtgat ttttgaggat
     5881 tttcctgttg ccctgtcctt gatttcaccg gcaaacccct tgaccttttc gacaccgctg
     5941 atcagccagt aaacaacgac aaatgtcgta atgacaaggc cccagaaggc aagtccatca
     6001 tcatataacg gattcagatt cggggaggct acaaaaatta taatgccaaa gaggatgaac
     6061 aggataacaa gtgaaatcaa tcttaacttt ttcatatttc cgccatttct ccatatggtt
     6121 caattcattt ccggccgccc gaagcggcag ccaagccaga tatatgttat catattcttt
     6181 tcgttttcgc aatcagtttc taacagaagg gccgccccgc gcttttaagc ggggcggccc
     6241 cggaatcagg catcctgatt gccgacagac tatgattgtt taaagatgtt cttccgacat
     6301 tttaatcatc cggcgcacca tttctccgcc gatcgggccg ccctggctgc cgttttcgcg
     6361 cgcagttacg tcgcctttat aatgatcgtt attttctttg acaaactgcg tacggcccat
     6421 ttcagacgcc acttccattt tgaactgatt catcacgtca tacgcttgag gattcaacat
     6481 tctggattta cggctgttcg acataattat cacctcgtaa ttattgtgat ccttattacg
     6541 gtcatttatg aaatgtaaaa aattcaaaat aagcaaaaat tcagccggca aggttgacga
     6601 atcgaggctg atcctgtata gtaatattgg tgttattcag tgggagatgt tatgaaatga
     6661 tcaaaaacct cgtcgttctt gcggtaaagt tagtctaaac agggtaattc tacctttttt
     6721 tagaaattca ccgtaaacgg cagggtataa aacttacaat gctgcacagg cagaaggttt
     6781 tattaccttt gacctgtgtt ttttatttat ccccgacaga ttacaaacaa ttttaagata
     6841 acagaggaat accgcaatga gcatattaga tgtaaccggg ttaacccatt attttggaga
     6901 taaaaaaata ttcagcgata cgggcctttc tctttttcga ggcgataaaa tgggtctgac
     6961 cggcctgaac ggagccggga aaagcacact gattaacatt cttgccggaa ccgtgatacc
     7021 ggatcagggc tatattaaat ggaaccccaa ggtcagagca ggttaccttg atcagcaggc
     7081 caacattgac ggctgccaga gtgtcaggga ttatttaaag ggtgcgtttg accatcttta
     7141 ccaaacagag acgcttttag aagaaaccaa cgctaaaatt gccgcctgca aggatcgtac
     7201 cgagcttgaa aagcttatgg ccgtctcggg ggattgtcaa aaccttttgg aaagcaacaa
     7261 tttttacggg ttggaaagcg aaatcgacaa agttgccgca gggctcggca ttaccgcctt
     7321 tggaaccgac aaaccggttg aaaagcttag cggcggtcag cgggcgaagg ttatgctggc
     7381 caagcttctg ctggaaaatc ccgatgtgct gctgctcgac gagcccacca actttctcga
     7441 ccgggaacat attgaatggc tgggtaagtt tttgattaat tttaaaggct cgtttcttct
     7501 tgtttcccac gatttcccgt ttcttaaccg tgtggtcaac tgtatttgca acatcgagtt
     7561 ttttaccatt acccgctaca acggcagttt tgaaagcttt ctcaaacaaa aagagcagaa
     7621 agccgaggaa tatcagagaa gatacagccg ccagcaaaag gaaattgcca aactggagga
     7681 ttacatcggc cgcaatcttt accgcgcgtc caccacggca atggcgcaaa gccgccgcaa
     7741 gaaactcgaa aaaatggaga aaattgaccg gccgcagcag gccgtaaagc cgacctttac
     7801 gtttaaatat cagtccatcc ctcccaaaat catgctgcgg gttaaagatc tggaagtagg
     7861 gtacggggag ccgctcctgc caaagatcgc gattaatctc agaacgggcg aacgcctcag
     7921 cgtttccggt ttcaacggca tcggcaagac cacctttctg aagaccatcg ccggtctgct
     7981 tccaaaaatc tccggcagct tcaaatacga cccaaacgtc gttatcggct attatgagca
     8041 ggaacaccac tgggaagacc cctcccggac ggctttcaac caaatcaggg agctgtttcc
     8101 aaaacttaag gataaagacg tccgggggaa tctggcaaaa tgcggcctga aacacgagca
     8161 ggccatgcaa agcctttcca ctctgagcgg gggcgagcag gccaaggtca agctttgcat
     8221 cctgacactg acgccctgca acctgctgat tctcgacgag cctaccaatc atctcgacca
     8281 aaacgctatt gagcatctga aacaggcgat catggatttt cccggcagcg tgatctttgt
     8341 ttcccacagc aaggaattct gtgaagcgct ggccgacaaa cagctgaaca tggaaaaact
     8401 gtttgactga acaaagaatt taatcgaatt aattaccata ttctgacaat ttatttaagc
     8461 cgtatccatt aaaatatcct ttgaaaccgg tcatcggtct caaaggatat tttttagcac
     8521 agcaggaagt ctttgcgtat cattcggggg cctccgggaa tcatgacagt ataggacggc
     8581 ccttttatga ctctatgcgg ggtgaataaa tggaacatat ggtgtattac gattcccggg
     8641 aaaagctggt gcagtggaag ctggatgaaa cgctttacag tttaatcagt aaggttgaca
     8701 aaagtttccg tcagatctgc gtaatctgta tcggaaccga ccgttcaacc ggtgacagct
     8761 tcggcccgct gaccggtcac atgctttcaa gactgaccgc atttcatttt aatctttacg
     8821 gaacctttga agcccccgtc cacgcgctga cactccccga gacactcgac cggatcaatt
     8881 caaaggattc tctggtcatc gcagtagacg caagcgtcgg caacggcgag caggtcggct
     8941 atatcggcat ggggtacgaa cctgtccgtc cgggcagcgg gcttgggaaa gagctgccgc
     9001 cggtcggtga tatctccatt accggcatcg tcgccatggg agggctcgcg ccgtttatca
     9061 tgctccagaa cgcctcactc ggcatggttt atcatatggc ggaaaaaacc tttctcgcca
     9121 ttcaatattc gctttacaaa ctgcatcagc gggaaattgc tccggccgaa aatgatcggg
     9181 acctcattcg gaaaccctca gcttagccct cgatatcctg gaaattttca tttccgagcg
     9241 ttgtatgata aagctccgac ttccggacgc cgagacgcgc ggatgccgtt tttaccgctt
     9301 ccgagtggct cattccttcc ccgaccagtt cccggaccag ctgcgccgcc tgctcgatcc
     9361 ggttttcttc gccatcctcg gtttcctgcc tgatcatacc ttccacaacc aaaacaaatt
     9421 cccccttggg ggtgttttga gcgtaaaact caacggcttt ggaaatggtc gtccggacga
     9481 cctcctcatg catctttgtc atctcacgca caagggcgat ccgcctgtcg ccaagcccgg
     9541 ccagcaggtc gtcaagcgta gtccggagtt tatgcggcgc ttcgtaaaaa atcagggttc
     9601 tttggtcttc gcgaaccgac tgcaggtgct ccctgcggct ttttttattt acgctcaaaa
     9661 agccctcgaa gctgaatctt ccggtgggaa gccccgatac ggcgagcgcg gaaaccacgg
     9721 cgcaggggcc cggcacaatg gtcaccttta tcccgttatc ggcgcagaga gccacaagat
     9781 cctcgccggg atcggaaatg gcgggcattc ccgcatcggt taccagcgcg caggtttcac
     9841 ccgcctcaat acggcggacg atctcttcac cgcgcagttt tttattatgc tcaaaatagc
     9901 tgatcatcgg ctttttgatc tcaaaacggt tcagcagttt tatcgtcact ctggtgtctt
     9961 ccgcagcaat aaagtcggca tcgcgaagcg cctgctccgc ccgggtggaa aaatcgccga
    10021 gattgccgat tggcgtagcg actaaaacaa gttgtccctg tgccatcata tcccccgttc
    10081 ccctcttttt attatttgat aaattcagcg ccgtatattc tccgcagctc catgccgggc
    10141 gaaccgtccg gctcctcgaa gagcaataca ggctccaccc tgagcccgcc gggggaagcg
    10201 ttctttctgc cctccgcaag aaacagccat ggttctgagc cggctttttg ctgcacgaac
    10261 cgcagcctct tcggctccac ccggtatcgc gccatcaggg acagaatttc ggcaaggcga
    10321 cccggtctgt gacagaggca c
//
LOCUS       DATALY010000011        17270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_88665,
            whole genome shotgun sequence.
ACCESSION   DATALY010000011 DATALY010000000
VERSION     DATALY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88665"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VHR42_00300"
     CDS             <1..808
                     /locus_tag="VHR42_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3025660.1"
                     /translation="KSQSENLMNELKQQMTGQIGRILQQSGAAGAVRSGSSSLRTPEL
                     TMPSVTFKLEVPQSRFIYLNGSDTMTAFDSIAAMMMGFFIFFFVFLIAGVSFLHERIS
                     GTMERLLATPIRRHEIVMGYFIGFGIFVVAQTLLIQLFMVYALHIPLKGNFLLVLLVN
                     VLLASGSLSLGTFLSAFARNEFQMIQFIPIVIVPQILFCGLFDLRAAPLWVTVLSKLF
                     PLTYGADALNSIALRGMGVSSIANDLAVMGVFTVLFLILNTLALKRYRKI"
     gene            complement(930..1880)
                     /locus_tag="VHR42_00305"
     CDS             complement(930..1880)
                     /locus_tag="VHR42_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HEX3025661.1"
                     /translation="MKNKVIKIFGYLITIVAFVFLIKSIIEKHPDFSKITHPFAAVGL
                     GLLITVMFVFIVYVSAYAWKSILEFIQGGKIHEPSLLSVYTKANIGKYLPGNFMHFAG
                     RNVLAGKLGFTQLDIAFSTIIEIAVLFLTACFWSLLLAFQYFVKVARQSLDLIASHKV
                     ISAIAIIILVLAICGFLFLMFRKDSFRKYRRFLTRGFLFLVLKLFIIYSFTLLLPAVS
                     FVVILPAVFGCSMTPYVFMLTTAAYVTSWVAGYMVPGAPGGIGVRESVLLLVLGASFP
                     SDILLIAIIFHRLLSVAGDGMAFLFDTLRNKVRKQQESKI"
     gene            complement(1900..2913)
                     /locus_tag="VHR42_00310"
     CDS             complement(1900..2913)
                     /locus_tag="VHR42_00310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3025662.1"
                     /translation="MLIPEIFEKLSNQTVLFITTKNLDYLRNTQEINLLRGHAAHVDV
                     IGSKKSSYLFRLISVYRRLATTSFKRYNVVFIGFAPQLIIPFFSWKLKGKTVFEDFFI
                     SLYDTFVFDRKKISPKSLIAKLLYRLDYKTIHAADAIISDTRADSRYFSKTFNMDSKM
                     LQVLYLEADTEYYKPQPLAEKPTDKFTVLYFGSILPLQGIEIILECARIMSDHDNIAF
                     DIIGPVEKKLMKQYGSLPNTRFTDWLPQKDLADRIARSDLCLAGHFSSEIDKASRTIP
                     GKAYIYRAMEKPMILGDNPANHELFQEKNSSIFYVQMGSAPKLKEKILFAMDQLGYKG
                     GNK"
     gene            complement(2919..3890)
                     /locus_tag="VHR42_00315"
     CDS             complement(2919..3890)
                     /locus_tag="VHR42_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3025663.1"
                     /translation="MKLIIQIPCFNEEKTLPETVRDLPKSIPGIDEIEYLIIDDGSRD
                     RTVEVAKEIGIHHVVKFKRNKGLAYGFMAGIDACLRLGADIIVNTDADNQYRGSDIGK
                     LVVPILENTADMVIGSRPIDEIEHFSKQKKMLQHLGSWVVRCASKTDVPDSPSGFRAY
                     SRDAAMRLNVVNHFTYTLETIIQAGRTKMAITSVPIGTNPETRKSRLFKGMTNYIKMA
                     STTIIRSFLMYNPLKFFSLIGGGLSVFGLLLIIRFLILYAIGNGSGHIQSLIFSVIFI
                     ILGFLTVIMGLLADLIAANRKLLEDIQYRVRKIDYNSDSSKSTDEKD"
     gene            4263..5213
                     /locus_tag="VHR42_00320"
     CDS             4263..5213
                     /locus_tag="VHR42_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX3025664.1"
                     /translation="MILKSVIMDCDPGIDDAVALFLLLASEKVDLLGLTACAGNQIPE
                     KTYQNARKLLALAQREDIPVFRGAPKPLIRELLIAGDVHGDSGLGGAALPESGAVIRS
                     EKAWDFIAETVAESSEQVTLLATGPLTNIAILLLCAPEIKSKINEIVLMGGACFGGNI
                     TPQAEFNVFVDPEAAKIVFNAGIPIKMFGLDVSTKAQMYRKEVECLKGYGSRTGAFFH
                     DILTFYNKTTIPYFLAPEGQEEGVHLHDPCAAAYLIDPSLFVLRDCFVEVETKDGPTL
                     GSTVVDYDRLTGKPFNASVAFGVDRERLIGLILESLKKFR"
     gene            complement(5300..5848)
                     /locus_tag="VHR42_00325"
     CDS             complement(5300..5848)
                     /locus_tag="VHR42_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3025665.1"
                     /translation="MKVLIISGTPKKEGLAFSCAEAAKKGVESAGGVCEIIRLCDQKL
                     IRCAMCGDGWGECLEHYTCKYGDDGFSGIQQKIADSDAVVLTSPVYWGDMSEVTKAFF
                     DRFRRCEARKGDDGAMAGKPVLLIASPGGSGAGAISCLEQMERLCHHLRAKVFDYVSV
                     NRWNKDYKLETITRAAEAMASI"
     gene            complement(6022..6975)
                     /locus_tag="VHR42_00330"
     CDS             complement(6022..6975)
                     /locus_tag="VHR42_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3025666.1"
                     /translation="MNIISTIGLEEKLRKQLVERCPNDVIRHEQLSSLSDGELKEIDG
                     LITFGNDIPVPVIDKMPKLSWIHIAQSGINDLPFDKLIEREIIVTTSKGINSITIAEY
                     VIGMMINVIRNNYTFYQAQKRGEWDFNTTLDEIYGRTLGIIGLGMVGKEIAKRGKALG
                     MRVIALEKFKIENPENVDQLILHDEKELIFRESDFIVTSLPLTDETLHFVDEKELSLM
                     KPTSYLINVGRGPVVNESALINTLKARKIAGAVLDVFEVEPLPKESELWSLDNVILTP
                     HVAGDRQSSYYQRVLDLFYYNLEKYPESGKMKNVPNLKLGF"
     gene            complement(7011..7742)
                     /locus_tag="VHR42_00335"
     CDS             complement(7011..7742)
                     /locus_tag="VHR42_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3025667.1"
                     /translation="MPDFLYNDISEQFEKKILSGSLKQGDKLPSERIMAQEYGVSRNV
                     IRESLRLLSEKGLIETRSGKGVYVIFPNEKKIADSLERVLISNKSTLMDMLEVRETLE
                     LSIIQKAVVLAKPADIDTLKEICNRLDEEKNDLSKFVEDDSRFHIQIARCTNNSIYPI
                     LVSAFYKITDNQLFLFTKINPNTVDATQADHRRLTEAIENKDAGSAERIMKSHLDGIR
                     TDLSALEQRSGISAGIEIEKTKLDD"
     gene            8043..8972
                     /locus_tag="VHR42_00340"
     CDS             8043..8972
                     /locus_tag="VHR42_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX3025668.1"
                     /translation="MAFKVILYQVILMLILMLLGTLLVKIKMLDDKGVSSLTEILLNV
                     VSPCVAIISFQLKFSSSLLRGLLFSAAAAGLIHIVSIVIGTCVFNKKIVDGHHGKILK
                     FASVYSNCGYIGIPLLAAVMGTQGIFYASVYGIVFTIFQWTHGVLLFTGKTDRKALVK
                     ALINPNVIAIVIGLFLFCFSIKLPALLYDSMNYVFNLNTPLSMIVIGSHLAQIDFRHV
                     FKDKWIWPGVALRNLLIPFAAIFLLHLAGAKGTLLMACLIPIACPVAGTTVLFSDLFH
                     EDAKFPTLLMAASTVLSIVTIPFVVYLVSALRF"
     gene            9195..9872
                     /locus_tag="VHR42_00345"
     CDS             9195..9872
                     /locus_tag="VHR42_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3025669.1"
                     /translation="MTKILFVEDDKTIAMGVEYSLRQEGFEVTLAFRFEEALKQLGNP
                     YDLILLDLGLPDGNGYEICRLAHADGTPVVILTARDEEVSVVQGLDMGADDYITKPFR
                     LRELISRLRAVLRRRQPDGAPGRNLLRCGGIAVDTQTMKVTKDYRDIPLTALEYRLLL
                     ILLTNKGRVLTRERLLECIWDVAGDFVNDNTLTVYIKRLREKLEDDPQNPSVIKTVRG
                     VGYVAGE"
     gene            9874..11109
                     /locus_tag="VHR42_00350"
     CDS             9874..11109
                     /locus_tag="VHR42_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3025670.1"
                     /translation="MNNRIYGEKKEWGVAALFAVTATALVLSVLLSILAVTYANRSTQ
                     DSYARITAAVCRQYPNAERAVVREIQNSANSNPAEGYRILGRYGLKELKISNSGAASE
                     MIGYLLPRELAFTGLFCAAFLFLEIRSRIHTRRGLNSLTEYLRRIHKGKDSLDLRDNG
                     EGSFSILKNEIYKITVMLREQAETLKRDKTALADSIADISHQIKTPLTSLFVLEDLLT
                     SDPPEDARREFLIRLRQQLERIQWLISSLLKLSRLDAGAVELKRDRVLFEDLMKKAAE
                     PLEIPADIKNQRIQVSGSPSAGFTGDLGWSAEAVSNILKNCLEHTPEGGEIQVKFEEN
                     TLFSALTITDNGEGVAPEDLPHIFSRFYRGKNASENSVGIGLAIAKAVIQAQGGEITA
                     GSETGRGTTFEIKIYRGVI"
     gene            11204..11890
                     /locus_tag="VHR42_00355"
     CDS             11204..11890
                     /locus_tag="VHR42_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3025671.1"
                     /translation="MEILKVEDLSKIYGKGETQVKALDNVSFTVQKGEFVAIIGPSGS
                     GKSTLLHILGGVDRPTSGKVMIDNTDIYALDPTRLAIFRRRQIGLIYQFYNLIPILNI
                     EENITLPLLLDGQKVDAGRLSEILQTLGLTDRVSHLPNELSGGQQQRVSIGRALVNRP
                     AIVLADEPTGNLDTNNSKEIIELLKYSNKHYDQTLIVITHDQKIALQADRILSIEDGR
                     IVRDVEVNHR"
     gene            11887..13596
                     /locus_tag="VHR42_00360"
     CDS             11887..13596
                     /locus_tag="VHR42_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3025672.1"
                     /translation="MNILKSYTLKYLRLNRRRTVVTIIGVILSCGLLCGTAVLAASFW
                     NMGIRTAIANSGNYHALFSGVPSAKAGYLTENSAVQTGMTSRVLGFSALQNSRNPYKP
                     YVFVVGYDANMLKTQPLKLVSGRFPQKSGEIVVSQDMISEKSEQVKAGEKITLSIGRR
                     VKQGETLSTSDSFETGERLAGIKRETFTVTGVIQKPEYQDYSCPGYTAIAYLDPNQLA
                     PSDQVDVSLLLKNPVKAFQTVPKMAQEAGIKVSSEYNSGAVTLNYNTSLLTWLGARTN
                     SSYTFSMAIIVAVIVLIIMIGSIGVIYNAFSISVGERKKQFGMLASVGATSRQIRRMV
                     FFEALIIGAVGIPFGILGGIAGIAAVISIASGILKSVLSNYETLRLTVSPAVIAVTLL
                     TASLTIWISALIPAKRASKVSPIDAIRQSSEYGNIKRRKLKSTGLAGKLFGFEGELAI
                     KSLKRDRKKYRTTVFSLFISIVMFVSFSSLMLYSFQETGTKSLPKYDFSAYLNNSGKV
                     DYTDFIRQAQKLSEVKECEVIRSIDGVVQTDSAGLSTEIKKLPDLIYGLDSKTDEKSK
                     SSC"
     gene            13615..14538
                     /locus_tag="VHR42_00365"
     CDS             13615..14538
                     /locus_tag="VHR42_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3025673.1"
                     /translation="MTLGKEAFAKYVKALGLNEADYENTNRPEGIFVNRNSIYMTDQT
                     THQNKHYVFDMLRLKTGGTVAVSQQKMSENDRPKAIDITVGAVDNRIVQDDMPSHGVY
                     IYVSDTVYNSLASKFNKISISNTITMNFSTRNNGKFDTEIKNLYTRLYGGTLSCSNTM
                     TERRNTQMMVWLVDLFCYGFITLITLIGVTNIINTINTSVRLRKREFAVLKSVGLTPK
                     GFEKMLRCESLFYGLKALLYGLPVSIGISYLLYRQFASGFQDFQRFSLPWGALSGCVA
                     GVFVIIFATMMYASVKVKDENIVDAIREENL"
     gene            complement(14737..15441)
                     /locus_tag="VHR42_00370"
     CDS             complement(14737..15441)
                     /locus_tag="VHR42_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3025674.1"
                     /translation="MELFAVKYGESYYDEKYIYHDMASSNGKTKFMWMFYLAKYQEKI
                     LLFDTGFRDATLAATYQLERKEINRELQNIVGGPLKADVVFLTHSHFDHIGNLDLFEH
                     SEIIISEKEYRTALEKCSEPVKNRLKSNRVIRVKEQYLFEEKFNFKVIGGHTFGSSVI
                     YFKENGKHYVLTGDECYMCDNLTQLRPIGDFTDLQKNKKFIENTYENRIIPLPFHDGK
                     IFETYKHVSENIAQII"
     gene            complement(15593..16354)
                     /locus_tag="VHR42_00375"
     CDS             complement(15593..16354)
                     /locus_tag="VHR42_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3025675.1"
                     /translation="MNSSTVFFRKEWKEQIRTMKGIVILIVLLLFGMTSPLMAKLTPE
                     IIKMAGAGIDIRVPTPTFLDAYAQFFKNIGQMALIVLILVFSGGVVTETSKGTAQMML
                     TKRLSRSGFILSKFCCYSLVWTISYAAAAGLCIAYTVFLFPDGRPQNLFLSLACMWIY
                     GLLIIAASVFSSVLFNNYALAATGGFCLWILLGLLSSIPRLKDYAPCALGSMNTALIS
                     GSLTLSAVRIPLLTGLAAAALLLIGACLIFRNREL"
     gene            complement(16347..17243)
                     /locus_tag="VHR42_00380"
     CDS             complement(16347..17243)
                     /locus_tag="VHR42_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3025676.1"
                     /translation="MAHDEKVIKYGGQRTGDKGSVFGFLGLNGAGKTTTIRMITGLAK
                     PTGGTISVCGEKVRFGSSAANRHIGYLPDVPEFYGFLKPAEYLNLCGRLSGMEHGRVR
                     PKAAELLNLVGLQTANRRIGGFSRGMKQRLGIAQALIHEPELLILDEPTSALDPVGRK
                     EILEIIASLRGRVTVLFSTHILSDAQRVCDNIGILHRGKLALSGSLSEIEQKFAGQSI
                     RIGIHEPNRISELRQRLAELAYVKEIKDDGPSGLLLHCGDTDLLGQTICPILSELGLS
                     LLHFEQVESNLEDVFIEVIGNE"
BASE COUNT         4396 a         4009 c         4338 g         4527 t
ORIGIN      
        1 gaaaagccaa tcggaaaatc tcatgaacga gctcaagcag caaatgaccg gccagatcgg
       61 ccggattttg cagcaatcgg gcgcagcggg agcagtgcgc agcggctcgt cttctctccg
      121 gacaccggag ctgacaatgc catcggttac ttttaaactg gaggttcccc agagccgttt
      181 catttatctg aacggttcag ataccatgac ggcttttgat tcaatcgccg ccatgatgat
      241 gggattcttt attttctttt tcgttttttt aattgcgggc gtatcgtttc ttcatgaaag
      301 aatcagcgga acgatggaac gtctgctggc aactccgatc cgaaggcatg aaatcgtaat
      361 gggctatttc attggctttg gtatttttgt ggtggcgcag acgctgctga tccagctttt
      421 tatggtgtat gcgcttcaca ttcccctcaa gggaaatttt ttgctggtgc tgcttgtaaa
      481 tgtactgctg gcgtccggct cgctgtcgct gggaactttc ctgtcggcat tcgcccggaa
      541 tgagtttcag atgatccagt ttatccccat tgtaattgtt ccccagatct tgttctgcgg
      601 gctgtttgac ctacgggccg ccccgctgtg ggtgacggtt ttgtcaaaac ttttcccgct
      661 gacttatggc gccgacgcgc tgaacagtat tgccctgcgg ggaatgggtg tatcgagtat
      721 tgcgaatgat ttggctgtta tgggggtatt cacggttttg ttcctgattc tcaatacgct
      781 ggccctgaaa cgttatcgaa aaatttaaag cggtgataga atcaaatacg atgagaaccc
      841 ccgagacagc attttttaaa tgctgtctcg ggggtaatgt tttggagatg aaccgtatgt
      901 tgcgataaat tttccggcaa gaagtcacct tagatttttg attcctgctg tttacgaact
      961 ttgtttcgca aagtgtcaaa cagaaatgcc atcccgtctc cagcgacgga aagtaatcgg
     1021 tgaaagataa tggcaatcag cagaatatcg ctgggaaatg aggccccgag aactaaaagc
     1081 aaaaccgatt cccggacgcc gattccgccc ggcgctcccg gtaccatgta accggccacc
     1141 caggatgtca cataagctgc ggtggtcagc atgaaaacat agggagtcat ggagcagcca
     1201 aaaaccgccg gaaggataac aacaaaggaa acagcgggga gaagaagcgt aaagctgtaa
     1261 atgataaaga gctttaaaac caaaaacaaa aatccacgcg tcaggaaacg gcggtatttt
     1321 ctaaaagaat cctttcgaaa catcaggaac agaaaaccgc agatggcaag aacaagaatt
     1381 ataattgcaa ttgcactgat tactttgtga gaagcaatca gatcaagcga ctgacgggcc
     1441 accttgacaa aatactgaaa agcaagaagc agcgaccaaa aacaggcggt cagaaataaa
     1501 accgcaattt cgatgatggt gctgaaggca atatccagct gagtgaatcc cagtttccca
     1561 gcgaggacat tcctccccgc aaaatgcata aaattccccg gaagatattt tccgatgttt
     1621 gccttggtgt agaccgataa gagggaaggt tcatggattt taccgccctg aataaactcc
     1681 aggatggatt tccatgcata cgccgaaaca taaacgatga agacaaacat taccgtaatc
     1741 agcagaccga gcccaaccgc tgcaaacgga tgggttattt ttgaaaaatc aggatgcttt
     1801 tcaataatgg attttatgag gaatacaaag gcgactatcg taatcaggta gccaaatatt
     1861 ttaatcactt tatttttcaa tgtatcatct cgcccaaact catttatttc cgcctttgta
     1921 tcccaattga tccatggcga acagaatttt ttcctttaat tttggggcgc tgcccatttg
     1981 cacgtaaaaa atggagctat tcttttcctg aaaaagctca tggtttgcag ggttatcccc
     2041 caaaatcata ggcttctcca tcgcacggta aatataagcc ttgccgggaa tcgtacggga
     2101 cgccttgtcg atctcactac tgaaatgccc cgcgaggcac aaatcggaac gggcgatccg
     2161 atcggcgagg tctttctggg gaagccaatc ggtaaatcgt gtgtttggca gcgatccata
     2221 ttgcttcatt agtttttttt caacaggccc gatgatatca aacgcaatat tgtcatggtc
     2281 tgacatgata cgggcgcatt ccaatatgat ctcaattccc tgcagcggca aaatactgcc
     2341 aaaataaagc acggtaaatt tatccgtggg cttttctgcc aacggctggg gcttgtagta
     2401 ttcagtatcg gcttccaaat acaatacttg cagcattttc gaatccatat taaatgtttt
     2461 cgaaaaatat cgggaatcgg cccgagtgtc ggaaataatc gcgtccgccg cgtggatcgt
     2521 tttgtaatcc agccggtaaa ggagctttgc aatcaagctt ttgggtgaaa tttttttgcg
     2581 gtcaaagaca aacgtgtcat ataatgaaat gaaaaagtct tcaaaaacag ttttgccctt
     2641 caatttccag gaaaaaaaag ggataatcag ttgtggtgcg aatcctataa aaacaacatt
     2701 gtaccttttg aaagaagtcg tggccaacct ccggtaaacg gagatcagcc ggaaaaggta
     2761 acttgatttt ttagaaccga tcacatcgac atgtgccgca tgaccgcgaa gaagattgat
     2821 ttcctgcgtg ttacgcagat aatcaagatt ttttgttgtg ataaacaaaa ccgtttgatt
     2881 cgaaagcttc tcaaatattt caggtatcag cattcttgtt agtccttttc atccgttgat
     2941 ttcgaggaat cggaattata atcgattttg cgaacccgat actggatgtc ttcaagaagc
     3001 ttccggtttg cggcgatcag gtcagccaaa agtcccatga taacggtaag aaagcccaga
     3061 atgataaaaa taaccgagaa aatcaaagac tggatatggc cgctgccatt cccgatcgcg
     3121 tataggatca gaaagcggat aataaggagc aggccgaaaa ccgataggcc tccgccgatg
     3181 agcgaaaaga atttaagagg gttatacatt aaaaaggaac ggataatggt ggttgaagcc
     3241 atcttaatgt agttggtcat acctttaaac agtcttgatt tccgggtttc cggattggtt
     3301 ccgatcggaa cggatgtgat ggccatttta gttctgccag cctgaataat ggtttcaagc
     3361 gtataggtaa aatgattcac cacattcaaa cgcatggccg catcacgcga gtaggcgcga
     3421 aatccgctgg gggaatccgg aacatctgtt ttggatgcgc aacggacaac ccagcttcct
     3481 aaatgttgaa gcattttttt ttgcttggaa aagtgttcga tttcatcaat tggcctgctg
     3541 ccgatcacca tatcggctgt gttttccaga atcggaacaa ccagttttcc aatatcggaa
     3601 ccgcgatact gattatccgc atcagtgttt acaataatgt cggcccccag acggagacag
     3661 gcgtcgattc cggccataaa tccataagcc aaacccttgt tgcgtttaaa tttgaccaca
     3721 tggtggatgc cgatttcttt cgcaacttca acggttctgt cgcggcttcc gtcatcaatg
     3781 atcaaatact cgatctcgtc gattccggga attgattttg gcagatcccg taccgtttcg
     3841 ggaagcgttt tttcttcatt aaaacaggga atttgaataa tcagtttcat atttgacttc
     3901 ctttagcata gtctttctgt tttataatga ttccgcataa gcggcgttaa cggttgacgg
     3961 ctatatgtaa aagtattgac aagtattttc acatatattt ttggcaatta ccctaatgcc
     4021 tgtgaattgt ttcaatatca gcttgttttg aaggtttatc ggatggatgt taaagagtct
     4081 aaccaacgat atggtagcaa aatttattca attagtcaat gttaacgtaa gcagtaagcg
     4141 tgtcaatttg cagtctgacg cttaaatcgt tctatcaaaa cagaaacttc tgttcttgca
     4201 atgccctgaa ttcatagtat aattttcttt aagagcgaaa aacaaacaag acgaaagcgg
     4261 ggatgatttt gaaatcagtt atcatggact gtgacccggg aatcgatgac gcggtcgcat
     4321 tgtttctcct gctggcgagt gaaaaggtag atcttcttgg cttgacggcc tgcgccggga
     4381 atcagattcc ggagaaaacg taccaaaatg ccagaaaatt gctcgcgctg gcgcagaggg
     4441 aggatattcc ggtcttccgg ggcgcgccga aaccgttgat tcgggaactg ctcatagccg
     4501 gagacgttca tggtgattcc ggtctcggcg gggccgcgtt gccggaatcc ggcgcggtaa
     4561 tccgcagtga aaaagcgtgg gattttatcg ctgaaacggt tgccgaatcc tctgagcagg
     4621 tgacgctttt ggccacaggt ccgctgacca acatcgccat tctgctgctc tgcgcccctg
     4681 aaataaaatc aaaaatcaat gaaattgtcc tgatgggagg agcatgcttt ggaggcaata
     4741 taacacctca ggccgagttt aatgtttttg tcgatcccga agctgccaaa attgttttta
     4801 atgcgggtat tccgattaaa atgtttggcc ttgacgtttc cacgaaagca cagatgtatc
     4861 gtaaggaagt ggaatgcctt aagggatatg ggagcaggac aggtgcgttt ttccatgata
     4921 ttctgacttt ttacaacaag accaccattc cgtattttct cgcccccgag ggacaggagg
     4981 aaggagtgca tcttcacgat ccctgcgcgg ccgcatacct gatcgacccg tcgctgtttg
     5041 tcctgcggga ctgttttgtc gaagttgaaa caaaagacgg cccgacgctg ggcagcacgg
     5101 tagtcgatta cgacaggctg accgggaaac cgtttaacgc gtcggtcgcg ttcggtgtgg
     5161 atcgggagcg cctgatcggc ctgattttgg agtcccttaa aaagttccgg tagggaaacg
     5221 aatgacgcaa gaggcgaaca ccgtaaaatg cgagagaagt cctccggtta ccggaggact
     5281 tctgctttta gcggaaaagt taaatggaag ccatggcttc agccgcgcga gtgatggtct
     5341 ccagtttgta atctttgttc cagcgattga cgctgacata gtcgaaaacc ttggctcgca
     5401 ggtgatggca aagcctttcc atttgttcca gacagctgat ggctcccgct ccgcttccgc
     5461 cgggggaggc gatcagtaaa accggttttc ccgccatcgc gccgtcgtcg cctttgcgtg
     5521 cttcgcagcg ccggaaccgg tcgaaaaatg ctttcgtaac ttccgacata tctccccagt
     5581 aaacgggaga ggtcagcaca acagcgtcgg aatcagcaat tttttgctga attccggaga
     5641 atccgtcgtc tccgtacttg caggtgtaat gttccagaca ttcgccccag ccgtcgccgc
     5701 acattgcgca ccgaatgagc ttttggtcac agagccgaat aatttcgcag actccgccgg
     5761 cgctttccac accttttttc gccgcttcgg cgcaggagaa ggcgaggcct tcctttttcg
     5821 gcgttcctga aatgataagt actttcatcg ctgtcaactc ctttggattg aatcattcgt
     5881 atggtattac gataatacct gatatctatt ataatgctga acttacaaca gatatccttt
     5941 tgattcctga tctgatgaaa ggaaacaggg cgcggggcaa acaaagttta tcgtcagggc
     6001 cttatgtatc cataacggag gttaaaatcc gagtttcagg ttgggtacgt ttttcatctt
     6061 tcccgattcc ggatattttt cgagattgta gtagaaaaga tccagcactc gctgataata
     6121 gcttgactgc cggtcgcccg cgacatgcgg ggtgaggatg acgttgtcga gcgaccataa
     6181 ttccgactct ttgggaaggg gctcgacttc aaaaacatcc agaacggcgc cggcaatttt
     6241 gcgggccttc agggtgttta tcaatgcgct ttcgttaacg accggtcctc ttccgacatt
     6301 gatgagataa gaggtgggct tcatcagcga cagctctttc tcatccacaa aatgaagagt
     6361 ttcatccgtc aggggcagag aagtaacaat gaagtcgctt tcgcggaaga tcagttcttt
     6421 ttcatcgtga aggatcagct gatcgacatt ttccggattt tcaattttaa atttctccag
     6481 agcgattacg cgcatgccca aggctttgcc gcgtttcgca atctctttgc caaccattcc
     6541 gaggccgatg atgccgagcg tccggccgta gatttcgtca agcgtcgtat tgaaatccca
     6601 ttcgccgcgc ttctgcgctt ggtaaaaagt ataattgttg cggataacat taatcatcat
     6661 cccgattaca tactccgcaa tggtgatcga gttgattcct ttggaggtgg tcacaatgat
     6721 ttcccgctca ataagcttgt cgaacggaag atcgttgatt cccgactgag cgatatggat
     6781 ccagctgagt ttgggcattt tgtcaatcac cggaaccgga atgtcgtttc cgaatgtgat
     6841 caggccgtcg atttctttaa gctcgccgtc gctcagtgag gaaagctgct catgacggat
     6901 gacgtcattc gggcatcgtt ccactaattg ttttctgagt ttttcctcga gacctatggt
     6961 tgaaataatg ttcattgata aattccctcc aaatgaaact ctttggtttg ttaatcatca
     7021 agctttgttt tttcgatttc gatcccggct gagatgccgg atctttgttc cagagcggaa
     7081 aggtcggtac gaatgccgtc gagatggctt ttcataatcc gctccgcgct gcctgcgtct
     7141 ttgttttcaa tcgcctcagt cagtctcctg tggtcggcct gtgtggcatc caccgtattt
     7201 gggtttatct tggtaaacag aaaaagctga ttgtcggtga ttttataaaa ggcgctgacg
     7261 aggattggat aaatactgtt attggtgcat cgggcgatct ggatgtgaaa ccgcgaatcg
     7321 tcttcgacaa atttagaaag gtcgtttttt tcttcgtcca gcctgttgca gatttccttc
     7381 agcgtgtcga tatccgcagg cttggcgaga acgacagctt tttgaataat cgacagttcc
     7441 agcgtttcgc ggacttccag catatccatt aatgttgatt tattgctgat cagcacccgc
     7501 tccagcgaat cggcaatttt cttttcgttg gggaagatca cataaacgcc tttgcccgac
     7561 cgggtttcaa tcagaccctt ttcgctcagc aggcgcaggg attcccgaat cacatttctg
     7621 cttacaccgt attcctgggc cataatccgc tctgaaggga gcttgtcccc ttgcttaaga
     7681 gatccgctca ggattttttt ttcgaattgc tccgaaatgt cgttatacaa aaaatccggc
     7741 atcgttttca cccttctggt caaaccagta gtaccaattt cattctatca tatatcatac
     7801 cgcgattggt tgtcaatctg taaaatgaat caaaacatag gccggattag cgctccgctt
     7861 ttttgccttg acaattccgg aacctgtgtt aaaattaaac ggtatgaccg gtaagaccgg
     7921 tgtgcgcggc aagccggttt ggccgatccg cggcactgtt ccggtttcat agtacccgtg
     7981 atcagatctt tcattttacc acttttttta aaaaacattt attcattatc gggggattat
     8041 tcatggcctt taaagttatc ttgtatcagg tgatcctgat gttgattctg atgcttctgg
     8101 gcacgcttct ggtgaagatc aaaatgctgg atgataaagg agtcagcagt ctgacggaaa
     8161 ttctgctgaa tgtggtttca ccctgcgttg ccattatttc atttcaactg aaattttcat
     8221 cgagtctgtt gagggggttg cttttttcgg ctgccgcggc cgggttaata catatcgtat
     8281 ccattgtaat cggaacgtgt gtctttaata aaaagattgt cgacgggcat catggaaaaa
     8341 ttctaaaatt cgcatccgtc tattccaatt gcggctacat cggtattccg ctgcttgccg
     8401 ccgtaatggg aacccaggga atcttttacg cttcggtata tgggattgtc ttcacgattt
     8461 ttcaatggac gcacggcgtt ttgcttttca ccggaaaaac agaccgaaaa gcgttggtta
     8521 aggcgctgat taatccgaat gtgattgcca ttgtgatcgg gttgttttta ttctgttttt
     8581 cgattaaact tccggccctg ttgtatgaca gcatgaatta tgtgtttaat ctaaacacac
     8641 ctctgtccat gattgtcatc ggcagtcatt tggctcaaat tgatttccgg catgttttca
     8701 aggataaatg gatctggccg ggcgttgcgc tgcggaacct tctgattccg ttcgcagcga
     8761 tttttctgct gcatctggcc ggggcgaaag gcacgttgct gatggcctgc ctgattccga
     8821 ttgcctgtcc ggttgccggg actacggtgc ttttttccga tctgttccat gaagacgcca
     8881 aatttccgac tttgctgatg gctgcatcca cggtgctgtc catcgtaacg attccgttcg
     8941 ttgtctatct ggtgtccgcc ctccggtttt gaccggaagg aatggccgat agcacttttg
     9001 tgaaatagaa tcagcccgaa cggcaaacgc aggggaactt gtctgacaag tcacaccctg
     9061 cgttttttct gctttgtaag tgttacccat ggcttcagac cggatttgtg cggcctcgaa
     9121 tcgtacgtga tttttatgat tttagtaaga aaaagctgta taatagttga aaaggcgacg
     9181 tggggggata cggcatgacg aaaattctgt ttgtggaaga tgacaagacc attgccatgg
     9241 gtgttgaata ttcacttcgg caggaaggct tcgaggttac gctggctttt cgtttcgaag
     9301 aggccctgaa acagcttggg aatccttatg atctgattct tcttgatctt ggcctgccgg
     9361 acgggaacgg ctatgaaata tgccgcctgg cgcacgcgga cgggacgccg gttgttattc
     9421 tgactgcccg tgatgaggaa gtcagtgtcg tgcagggtct ggatatgggg gccgacgact
     9481 atattaccaa accgttccgg ctcagagaac tgatatcccg tctcagggcg gtgctgcggc
     9541 gcagacagcc cgacggagcg cccggccgaa accttctgag atgcggcggt attgcggtgg
     9601 acacgcagac gatgaaagta acgaaagatt acagggacat tcctctgacc gccctggaat
     9661 acaggctgct tttgatcctg ctgaccaata agggaagggt tctgaccagg gagcgtctgc
     9721 tggaatgtat ttgggatgtt gcgggtgatt ttgtcaacga caacacgttg acagtctata
     9781 ttaagaggct gagggaaaaa ttggaggatg atccgcagaa tccttccgtc attaagactg
     9841 tgcgtggagt aggatatgtg gcaggtgaat aaaatgaaca atagaatcta tggggaaaag
     9901 aaggaatggg gcgttgcggc cctgttcgcc gtgacagcga cggcgcttgt tttatcggtt
     9961 ctgctgagta ttctggccgt tacatacgcc aaccgttcca ctcaggactc ttacgcccgg
    10021 ataacggcgg cggtctgccg gcaatatccg aacgcggaac gggcagtggt gcgcgaaatc
    10081 cagaattccg cgaattcaaa tcctgccgag ggctaccgga ttctgggccg ttacgggcta
    10141 aaagagctta agatcagtaa cagcggtgcc gcgtcggaga tgatcggcta tctgctcccg
    10201 cgggaactgg cgtttaccgg tcttttttgc gccgcgttcc tgtttcttga aattcggagc
    10261 cggattcata cgcgccgcgg gctcaacagt ctgactgaat atctgagaag aatccataaa
    10321 ggcaaagatt cgctcgatct gagagataac ggcgaaggca gtttcagtat cttaaagaat
    10381 gaaatttata aaatcaccgt gatgctgcgg gagcaggcgg agaccttgaa aagggataaa
    10441 accgccctgg ccgattccat cgcggatatt tcccatcaga ttaaaacgcc cctgacctcg
    10501 ctgtttgtgc tggaggatct tctgacatcc gatcctccgg aggatgcacg gcgggaattt
    10561 ctgattcggc tccggcagca gctcgaacga attcagtggc tgatatcctc tctgctcaaa
    10621 ctgtcgaggc tggatgctgg ggcggtagag ctgaaacggg acagggtgct gtttgaggac
    10681 ctgatgaaaa aagcggcgga gccgttggaa attcccgcgg acattaaaaa tcaacgaatt
    10741 caggtttccg gcagcccttc cgccggtttt acaggggacc tcggctggag cgcggaggct
    10801 gtatccaata ttttgaaaaa ctgcctcgag catacccccg aagggggaga aatccaggtg
    10861 aagtttgaag aaaacacgct cttttccgcg ctgaccataa ccgacaacgg cgagggcgtc
    10921 gcgccggagg acctgccgca tattttttcc cggttttacc gggggaaaaa cgcttccgaa
    10981 aacagtgtcg gcatcggtct tgccatagcg aaggcggtga ttcaggcgca ggggggagaa
    11041 atcaccgccg gaagtgaaac cggcagggga accacctttg aaattaaaat ttaccggggc
    11101 gttatctgat gattggatca gtgacaaaat tgtcactctg caggtcaccg ggatgtcacc
    11161 ttgctgtcgt atacttggca gagaacaaaa gggggacgca ggaatggaaa ttttaaaagt
    11221 cgaagacctc tccaagatat acggaaaagg tgagacgcag gtcaaagcgc tcgacaatgt
    11281 aagcttcacg gttcaaaagg gtgaatttgt tgccattatc ggcccgtccg gttcgggcaa
    11341 atcgacattg ctgcatattc tggggggcgt ggacaggccg acctccggca aagtcatgat
    11401 cgacaatacc gatatttacg cgcttgatcc gacccgtctg gccatctttc gcagaaggca
    11461 gatcgggtta atttatcagt tttataatct gatcccgatt ttgaatattg aggaaaatat
    11521 tacgctgccc cttctgctcg acgggcagaa ggtcgacgcc ggacggcttt cggaaattct
    11581 tcagacactc ggccttaccg accgggtcag tcacctgccc aacgagcttt ccggcggtca
    11641 gcagcagagg gtgtccatcg gtcgagcgct ggttaaccgc ccggcaatcg tgctggccga
    11701 tgagccgacc ggaaacctcg acaccaacaa ttccaaagag atcatcgagc tgctcaaata
    11761 ttccaacaaa cattacgacc agacgctgat tgtgattacc cacgaccaga aaatcgccct
    11821 tcaggccgat cgcattcttt ccattgaaga cgggcggatt gtacgggatg tcgaggtgaa
    11881 tcacaggtga acatattaaa atcctataca ctcaaatatc tgagacttaa caggcgccgc
    11941 acggtggtga cgattatcgg tgtgatcctt tcctgcgggc tgctctgcgg cacggcggtg
    12001 ctggccgcaa gcttttggaa tatgggtatc cgcaccgcca ttgcgaatag cggaaattat
    12061 cacgcgctgt tttccggagt gccgtccgca aaagcaggat atttgaccga aaacagcgcc
    12121 gttcagaccg gaatgacgag cagggttctg gggttttccg cgctgcagaa ttccagaaac
    12181 ccctataagc cctatgtgtt tgttgtcggg tatgacgcca atatgcttaa aactcagccg
    12241 ctgaaactgg tttccgggcg ttttccgcaa aagtcgggcg aaatcgtggt ttcgcaggat
    12301 atgatttcgg aaaaatcgga gcaggttaaa gcgggcgaaa agatcactct ttccatcggc
    12361 cggagagtga agcagggcga gacgttatcc acatcggata gctttgagac cggtgaaagg
    12421 cttgccggga tcaaaaggga aactttcacg gtaacgggcg tcatccagaa accggaatat
    12481 caggattata gctgcccggg ctacacggct atcgcctatc tcgacccgaa tcagcttgcg
    12541 ccgtcggatc aggtggatgt ttccctgctg ctgaaaaatc cggtcaaagc gttccagacg
    12601 gttcccaaaa tggcgcaaga agcaggcatc aaagtgtcgt ccgaatataa cagcggggcc
    12661 gtgacgttaa actataacac ttcgctgctt acctggcttg gagcgcggac caacagcagt
    12721 tatacttttt ccatggccat tatcgtcgct gttatcgtgc tgattatcat gattgggtcg
    12781 ataggggtta tttataatgc tttttccatc tcggtcggtg aacggaaaaa gcagttcggg
    12841 atgctcgcaa gcgtcggggc gacgtcgcgg cagatccgcc ggatggtctt ttttgaggcg
    12901 ctgatcattg gcgcggtcgg gattccgttc gggatactcg ggggaatcgc cggcatcgcg
    12961 gccgtcattt ccatcgcgag cggaatctta aaatccgtgc tgtcaaacta tgagacgctg
    13021 cgccttaccg tgtcgcccgc tgtgattgcc gttacgctgc tcaccgcttc gctgacgatc
    13081 tggatctcgg cgctgattcc ggccaagaga gcgtccaaag tttcaccgat tgacgccatc
    13141 agacaaagca gtgaatatgg caatatcaaa cgccgcaaac tgaaatctac gggccttgcg
    13201 ggaaaattat tcggctttga aggggaactg gcgatcaaaa gcctgaagag ggatcggaaa
    13261 aaataccgta caacggtttt ttctttgttt atcagcatcg tcatgtttgt atcctttagt
    13321 tccctgatgc tgtattcctt tcaggaaacc ggaacgaaat cccttcccaa atatgatttt
    13381 agcgcctatc tcaacaattc cggaaaggtg gattacactg atttcatcag acaggcccaa
    13441 aagctttccg aggtaaagga atgcgaggta atccgaagca tcgacggagt tgttcaaacg
    13501 gattccgccg gcctgagcac ggaaatcaaa aagcttccgg atctaatata cggtctggat
    13561 tcgaaaacag atgaaaaaag caaaagcagc tgctagaacg tcgacgtggc cgtcatgaca
    13621 cttggaaagg aagcgttcgc caagtatgtg aaagcgctcg ggcttaacga ggcggattac
    13681 gaaaatacaa ataggccgga aggcattttc gtcaaccgga acagcatcta tatgaccgat
    13741 cagacgactc atcaaaataa gcattacgtc ttcgacatgt tgcggctcaa aaccggcgga
    13801 acggttgctg tttcgcagca gaagatgtcg gaaaacgacc gccccaaggc aattgacatc
    13861 accgtcggcg cggttgacaa tcgtatcgtg caagacgata tgccgagcca tggcgtatac
    13921 atttatgttt ccgacaccgt atataacagt ttggcttcca aatttaataa aatcagtatt
    13981 tccaatacga tcacgatgaa tttcagcaca agaaacaacg gaaaattcga cacggaaatc
    14041 aaaaacctat acacccggct ttacggcgga acgctcagtt gctcgaacac gatgaccgaa
    14101 agaagaaaca cacagatgat ggtgtggctg gtcgaccttt tctgctatgg ttttatcaca
    14161 ctcatcacgc tgatcggcgt aaccaatatc atcaacacca tcaataccag cgttcggctt
    14221 cgaaaaaggg aatttgccgt tttgaaatcg gttggcctta ccccgaaagg tttcgaaaaa
    14281 atgctccggt gcgagagctt gttttacggt ctgaaagccc ttctctacgg gctgccggtc
    14341 agtatcggaa tcagttatct gctttacagg cagtttgcgt cgggctttca ggattttcag
    14401 cggttttcac ttccctgggg ggcgttgtcc ggatgtgtcg ccggggtgtt tgtcatcata
    14461 ttcgcaacga tgatgtatgc ttcggtaaag gtaaaagacg aaaatattgt cgacgccatc
    14521 cgggaggaaa acttgtagag aatagaacaa aaggggctgg cccaaaatac cccatgaaaa
    14581 ataatggcac acaaaaaaat cagcccgaac aggcgttact caatgcttgt tcgggctttt
    14641 tgttttatct gatatttcag gtatccttac agcaattcca attaagatgc agaattggaa
    14701 tcgctgtagt ttgccgcact tgccatggcg gcagttctaa ataatttggg caatattttc
    14761 ggaaacgtgc ttataggttt cgaagatttt cccatcgtgg aaaggaagag ggatgatcct
    14821 gttttcatag gtgttttcaa taaatttctt gtttttctgt aagtctgtaa aatccccgat
    14881 aggccttaat tgcgtaaggt tatcgcacat ataacactcg tctccggtaa gaacatagtg
    14941 ttttccgttt tccttaaaat aaattacgga agaaccaaat gtatggccgc caatcacctt
    15001 aaagttaaat ttttcttcaa aaagatactg ctcttttacc cggattaccc ggttgctttt
    15061 taacctgttt ttgaccggtt cggagcattt ttccaatgcg gtccggtatt ccttttccga
    15121 gatgataatt tccgaatgtt cgaatagatc caagtttccg atatgatcga aatggctgtg
    15181 agtaagaaag acaacgtcgg ctttcaacgg cccgccgaca atattttgca gttcacgatt
    15241 gatttctttt ctctccaatt gataggttgc cgcaagcgtt gcatcgcgga atcccgtgtc
    15301 aaacaacaga atcttttcct gatatttggc cagataaaac atccacataa attttgtttt
    15361 gccattggac gaagccatat catgataaat atacttttcg tcataataag attccccgta
    15421 tttaacagcg aataactcca tgttttctcc catatcctta cgtcttaaaa tttaacaacc
    15481 ttgtttgaaa ccggctgact ttaataatca attataccac aaaattcaga ctttttaacg
    15541 acgggctcgg cctttgcggt tacggatgtt ttttatcgtc agatattttt atttacagct
    15601 cgcggtttcg gaaaatcaga caggctccaa tcagcagcag agcggcagcg gcgagccccg
    15661 taaggagggg aatccgaaca gccgagagtg taaggctacc gctgataaga gccgtgttca
    15721 tgcttccaag cgcgcaaggg gcgtaatctt tcagcctggg tatgcttgag agcagaccga
    15781 gcaggatcca gaggcagaaa ccgcctgttg cggcgagggc gtaattgttg aacaggacac
    15841 tggaaaaaac agaggcggcg ataatcagca atccgtaaat ccacatgcac gcaagtgaca
    15901 gaaacagatt ctggggtctg ccgtcgggga acagaaagac ggtgtaggcg atgcaaagcc
    15961 ctgccgccgc cgcgtaggaa atcgtccaca cgagagaata gcagcagaat ttcgaaagga
    16021 tgaaaccgct tctggagagc cttttggtca gcatcatctg agcggttcct tttgaagttt
    16081 ccgtgaccac tcctcccgag aacacaagaa tcagcacaat tagcgccatc tggccgatgt
    16141 ttttgaaaaa ctgggcgtat gcgtccagaa aggtaggtgt tggaacgcgg atatcaatgc
    16201 ctgccccggc catcttgata atttcagggg taagcttggc catcagggga cttgtcatgc
    16261 caaacagcag aagcacgatc aggatgacga tccctttcat ggttctgatc tgctctttcc
    16321 attctttccg aaaaaaaacg gtggaactat tcatttccga tcacctcgat aaaaacgtct
    16381 tccaggtttg attccacctg ctcgaagtgc agcagggaaa gccccagctc agagagtatc
    16441 gggcagattg tctgaccgag caaatcggtg tctccgcagt gaagcagcag gccggagggg
    16501 ccgtcgtctt tgatttcttt gacatacgca agctccgcga ggcgctgccg gagttcgctg
    16561 atccggttcg gttcgtggat tccgatccga atgctttgcc cggcgaattt ttgctcgatc
    16621 tcactcagac tgccgctcag cgccagtttg ccgcggtgta agataccgat attatcgcag
    16681 acgcgttgcg cgtccgacag gatatgggtg gaaaacaata cggttacccg gcccctgagc
    16741 gaagcgataa tttcgaggat ttccttgcgg cccaccgggt cgagagccga tgtaggttcg
    16801 tcaagaatca gcagctcggg ttcatgaatc agggcctgcg cgattccaag gcgctgtttc
    16861 attccgcgtg agaatccgcc gatacgccgg tttgcggttt gaagtccgac aagattcagc
    16921 agctccgcgg ctttcggacg gacgcggccg tgctccatac cgctgagtct gccgcaaagg
    16981 ttaagatatt ccgccggttt aagaaagccg taaaattccg gaacgtcggg cagatagccg
    17041 atgtggcggt tcgcggcgga ggaaccgaac cgaaccttct cgccgcagac ggaaatagtc
    17101 ccgccggtgg gtttggcaag ccctgtaatc atgcggatgg ttgtggtttt tcccgcgccg
    17161 ttaaggccga gaaacccgaa gacactgccc ttatctcctg tccgctgccc tccatatttt
    17221 attacttttt catcgtgcgc cacgacaaaa aatcagaaac aataaaaaaa
//
LOCUS       DATALY010000012        12653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_88866,
            whole genome shotgun sequence.
ACCESSION   DATALY010000012 DATALY010000000
VERSION     DATALY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12653
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88866"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2568
                     /locus_tag="VHR42_00385"
     CDS             <1..2568
                     /locus_tag="VHR42_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3025677.1"
                     /translation="RRNYVVRKALYDKLMQCADMGVIFVRGGAGTGKTTLLSSFIRET
                     GLKNVGWLSLDASNTNVYSFWLYFTAAVNTFLEDDDGFLTLMRSNMDASHMESLLTIL
                     INRLCGEQDYYLVLDDVHCLNDAALIRTFEYFISAMPENFHLFMLSREEPPVYLGTLA
                     VSGRLLFIDGRQMQLSPEEGLTFLKQTMRMTGSDEELNTLNTYAEGWIGGLQLAAAAE
                     AAGKHSGELLRAGGGIAAEYLTREIYGALTQNEQDFLIGTGFLSYFDAELCTHLFDGF
                     TKAQFDEMIETLIKKNLFIICIDEQNGVYRYHNILSEYLTHRFLGITPEDRKIYYARA
                     AKAFEQRGDCEEALRQYCAADDYENVLRVAQSMGGKIEAWSYLDKVPADQLIQDADLA
                     AQSFLYNLGNLNVERCRVLYEKFKEYYGDSDVFKIMQFAEAYVSSGEAILPEYHALTA
                     EQIDGLSFGPVAKAMILVENSAALEERMQYEEAKNCLDRAIQVCAGTNAFVDFFAYNQ
                     MAQLNEELGRLNDSLACYERSKEMFKSPSMMSGIGTNFYFGIAGVYMRRMELDKAAET
                     LEQSRRLLEEQRIHVDITDMTLTYHLAEMKFLSGDAESGASYVNEILSEYPAFNVINL
                     GRLIHELDCAGLLPTELADEFLKEIEKASNYKGQPFFRLLRARILFEHGDTAEAFKET
                     EYVLTFSRAHKNQLRLVEAGLLKIFMLLRCSETASGHRETSNLLREAVYYAHKDRILM
                     PFYLDRAVLLPLLRGLITQSAGKSFMSVAEITFVRDAIALCDNTVTAARKPETLSARE
                     LEVLGELAKGITNREIAEKLCISQATVKTHLLSVFGKLGVTSRVMAVKVGQKKGLIL"
     gene            complement(2756..3994)
                     /locus_tag="VHR42_00390"
     CDS             complement(2756..3994)
                     /locus_tag="VHR42_00390"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX3025678.1"
                     /translation="MDKGQELTNVYSQYSDQALRKILYVSPETYEEHALSAAKNELCQ
                     RFEAFFSTYSGIPIPFRVILREVTFNEVSDELLLYIQKKSLIQEYKQVFEKLLLIESD
                     LDTSFGLNVLQSKNGKLNVEVIDISSNELVISDLLTWNEWLNAKVKQEQILKYGIERI
                     IAISLIKMTTNGFYEGESRQQLSEMEKYYNGVKDVKGDKQLTQIGSQDESYFLAHGIL
                     EYRAEKKQKPIQETLNHDEEYKRGKVPQVRPWVRFFARMIDYVLIYSIIIFFLKIMTP
                     GLFALYQSVTYISIPALVWLIIEAILISTIGTTPGKWILRITVRNSDGSKLNFKNSLL
                     RSLMVFSCGYGLSLPYVNIITSIISFIYLKSNKKTLWDKKMNLQVTHEKIDTWRGSVA
                     AVVLIFLPVLIFFFIKKHII"
     gene            4404..5012
                     /locus_tag="VHR42_00395"
     CDS             4404..5012
                     /locus_tag="VHR42_00395"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEX3025679.1"
                     /translation="MTTEYIPAAEQKTAKWSGGTTTELYIYPRTADYKNRDFLFRLSS
                     ATVDDAVSSFTKLDGYDRTLILLSGSLRLVHKNIREATLGQYDKDCFYGGYETTGYGS
                     GVTDFNLMAKEGCPAKAELCRMPAVGRMPVSVDRETHAIIGFYALSGDIRINCGGEKW
                     KLADKDFFTVEKESGEKLEIEALSFPGRLFVLSVLTINETAV"
     gene            5088..5951
                     /locus_tag="VHR42_00400"
     CDS             5088..5951
                     /locus_tag="VHR42_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3025680.1"
                     /translation="MAIAENSDFEIFTQKLIEISKESPTYEKLAYYIEKNYLRLIFMT
                     AGELASELNISQGSVSRFFITLGFRGYNDFLRSLQKIVSAKITAPQRLEYTAAGTKID
                     STISVEVGNMCELDRLMHEPAYHKMIDCLISDRDLFILSARMSATLVPYMRYILCKMR
                     DSVYEVTPDSIAWDTINLREPSKTEIFTIAFPRYPKTLVDKIKNLHDSGFHISAITDS
                     QLSPIIPYCDNVIDVPITVSSIFDIYSTPMAFINLMLRDAAKEIPSLEDRLNRIEKNE
                     GDSGAYYKRGK"
     gene            6268..7530
                     /locus_tag="VHR42_00405"
     CDS             6268..7530
                     /locus_tag="VHR42_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX3025681.1"
                     /translation="MPNHTITVNRQRLSDNLSALAQIGKSPSGGLDRHFGSSADLEAR
                     RFLKALWQKAFGAEVITDAAANLWVKAGGLEALPPIAIGSHHDTVKNGGMYDGAMGVL
                     LATEAFERINEAGIRLRHPLLLAAFTGEEPNPFGFSTIGSKLLSGKLTHEKLKGGYDA
                     EDGVTIEEALKNAGGDFAAVDSVRMNPGELAAFIECHIEQGVILEGRGAPIGVVERIV
                     GIYREELLVKGEANHSGTTVMARRRDALTAAAQVVLAIEDAARSFESDTLVATVGRME
                     VLPNSANIIPGEVALTFEVRTPDENCKKKALEKLGKAFTMIIEERGVTIERRTALDQP
                     TAVMDARVVSALERASQGICKTLPGMVSMAGHDAAHMAKVTRSGMLFVRTPGGKSHCP
                     EETANLDDIEKAGNALLSAIILLDKELD"
     gene            7580..8860
                     /locus_tag="VHR42_00410"
     CDS             7580..8860
                     /locus_tag="VHR42_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3025682.1"
                     /translation="MASSGVVEQDGMIADIGDAAALKKQYPNTAEIRWDGLVMVPGTV
                     NVHNHSFQSLLRGIAADRPFLEWRDEALYKYSPRLTPEDLYNGALFAFGEMLRYGVTT
                     VCDFFYLHNDGLESDEAIIRAAHDLGIRLVLARTMYDWEGAPKGYVESVDKACEHTKM
                     LFEKYRHSDMVSVVPAPHSLHAASLEMIKAGHALAKELGTAFHIHVAEEPFEVEQIRN
                     QYGKRTVELLDDIGVIDESTVIVHGVWLDGGEIETLAKKGGKLAYCPSSNMFLADGIT
                     DIEKMLSLGVKIGLGTDGGCSNNRISVFEEMRMAPLLQKAYKHNALCVNYREAFKMGT
                     EWGGELLGLPVGILKKGYCADFVGIDRYDLSMQPVTERGGQLLPNIVYSMQPGAIRRV
                     VAGGVERAADGHLSSIPERTVIERVKETMSRIER"
     gene            8956..10983
                     /locus_tag="VHR42_00415"
     CDS             8956..10983
                     /locus_tag="VHR42_00415"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX3025683.1"
                     /translation="MNFNEQAKEAMQIMLDDGLPEMPEFADGIRRAPSRGFHLTKEQT
                     KTALKNALRYIQPKYHKELIPEFLNELMTYGRIYGYRFRPQGRIYGRPIGEYRGNCIE
                     GKAFQVMIDNNLDFEVALYPYELVTYGETGSVCQNWMQYRLIKKYLEVLTQDQTLVVE
                     SGHPLGLFKSKPTAPRVIMTNALMIGAFDNEADWSVAEEMGVANYGQMTAGGWMYIGP
                     QGIVHGTYNTLLNAGRLKLGIPNDGDLAGRLFISSGLGGMSGAQSKAIEIANGVGIIA
                     EVDGSRIATRHDQGWVGVVSDNLDEVFRIAKEHLEVQKSVSIAYHGNIVDLLEYVVKN
                     KIKVDLLSDQTSCHAAYEGGYCPQGVTFGQRTELLATDRDKFCGLVDKSLRRHYELIK
                     SCVANGTYFFDYGNSFLKAIYDAGVKEISKNGIDDKDGFIFPSYVEDIMGPQLFDYGY
                     GPFRWVCLSGTDGDLETTDKAAMSCINVEGRYQDRDNYNWIRDAKKNRLVVGTKARIL
                     YQDAEGRMNIALKFNEMVRNGEIGPVMIGRDHHDVSGTDSPFRETANIRDGSNVMADM
                     AVQCYAGNAARGMSLVTLHNGGGVGIGKSINGGFGLVLDGSERIDEIIKCSILWDVMG
                     GVARRSWARNEHSIETSIEYNRKNAGQSQITLPFIPDEELVAKIVEAALKK"
     gene            10985..12235
                     /gene="hutI"
                     /locus_tag="VHR42_00420"
     CDS             10985..12235
                     /gene="hutI"
                     /locus_tag="VHR42_00420"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX3025684.1"
                     /translation="MGNMLILNAGEIATPIGRTALRGAQMNAIKTIPNGSVAVINGKI
                     AAVGPTDEIEREYGRKGLPVIDAAGCCVVPGFVDSHTHFIFSGYREEEFLMRLQKCSY
                     LDIMRAGGGIVNTVTHTRELSHDGLYQLGKERLHEMLAFGVTTLEGKSGYGLDLESEL
                     KMLRVQKQLDADGPCDIVASFLGAHAVAPEFKGRADDFINFLIESVLPVVTREKLAEF
                     CDVFCETDVFSVEQSQKLLLAAKKMGLTPKVHADEMSNLGGAQLACRVNAISADHLLN
                     VSKEGIQALAESETVATLLPATAFCLGMPYAVARGMIDSSCAVALASDYNPGSCFTNS
                     VPLILSLACIYMGMTAAEALTAMTINGAAAIGRADKIGSIEPGKQADLLILRYPSYKF
                     LIYNTAVNIVGTVIKNGRVAYQNL"
     gene            12254..>12653
                     /locus_tag="VHR42_00425"
     CDS             12254..>12653
                     /locus_tag="VHR42_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007084500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX3025685.1"
                     /translation="MVILTGNTLTLEEIDRIINRDEKVQAAPESMEKAAASRRAVERV
                     VSENRTVYGITTGFGKFSSVRIDPEDSDELQLNLIYSHACGVGEPFSPQVAKTMLLLR
                     VNTLLKGYSGVSLPVIDLMIELVNRNITPVV"
BASE COUNT         3307 a         2939 c         3347 g         3060 t
ORIGIN      
        1 cgcaggaact atgtcgtgcg caaagctttg tatgacaagc ttatgcaatg cgcggacatg
       61 ggcgtgatct tcgtacgcgg cggagccgga accggcaaga ccacactgct gtcctccttc
      121 atccgcgaaa cgggattaaa aaatgttggc tggctatccc ttgatgcgtc caataccaat
      181 gtctattcct tctggctcta tttcactgcg gcagtcaaca catttttgga ggatgacgac
      241 ggctttctta cgctgatgcg ttccaacatg gacgcttcgc atatggagag tctgcttacc
      301 atcctgatca accgcctttg cggcgaacag gactattatc tggtactcga cgatgtgcat
      361 tgcttaaacg atgcggctct gattcgcacc tttgagtatt ttatcagtgc aatgccggaa
      421 aactttcatc tttttatgct ctccagagaa gaaccgcccg tatatctggg cactctggcc
      481 gtttccggcc gtcttctttt cattgacggc cggcagatgc agctttcccc cgaggaggga
      541 ttgacctttc taaaacagac aatgaggatg accggaagcg atgaggagct gaatacgctc
      601 aacacctatg ccgagggctg gatcggcggg ctgcagcttg ccgcggcggc cgaagcggct
      661 ggcaagcatt ccggcgagtt gctgcgcgcg ggcggaggga tcgccgccga atatctgacg
      721 cgtgaaattt atggggcgct gacacaaaat gagcaggatt ttctgattgg caccggtttt
      781 ttgtcctatt ttgacgccga gctctgcaca catctttttg acgggtttac aaaggctcaa
      841 tttgatgaaa tgattgaaac gctcatcaaa aagaaccttt tcatcatctg catcgacgag
      901 caaaacggcg tttaccgcta tcataacatt ctttccgaat atctgacaca tcggtttttg
      961 ggcatcacgc cggaagatag aaagatatat tatgcaagag cggcaaaagc ctttgagcaa
     1021 cggggcgact gtgaggaagc cctgcggcaa tactgcgcgg cggatgatta tgaaaatgta
     1081 cttcgcgtgg cgcagtctat gggcggaaaa attgaggcgt ggagttatct tgacaaagtg
     1141 ccggcagatc agctgatcca agatgctgat cttgctgctc agtcctttct gtataacctc
     1201 ggcaacttga atgtggaacg ctgccgggta ttgtatgaaa aattcaagga atattacggc
     1261 gattccgatg tctttaaaat tatgcagttc gcagaggctt acgtttcaag cggtgaagcc
     1321 atcctgccgg aatatcatgc gctgaccgcc gaacagatcg acggcctttc ctttgggcct
     1381 gtagcaaaag caatgatatt agtggaaaat tcggccgcgc tggaagaacg aatgcaatat
     1441 gaagaggcaa aaaattgtct cgacagggcc attcaagtct gtgccggtac aaatgctttt
     1501 gttgattttt tcgcatataa ccaaatggcg cagctgaatg aagaactggg gcggctgaac
     1561 gacagccttg cgtgttatga aaggtccaaa gagatgttca agtcgccgtc gatgatgtcg
     1621 ggaatcggaa caaattttta ctttggcata gccggtgtct atatgcggcg gatggagctt
     1681 gataaagcgg cagagacgct tgagcaatcc cggagattgt tggaagagca gcgcattcat
     1741 gtggatatta cagatatgac gctaacctat catttggctg agatgaaatt tttaagcggc
     1801 gacgccgaat ccggcgcatc ctatgtgaac gagattttat ccgaatatcc ggcgtttaac
     1861 gtaattaact taggtcggct gattcatgag cttgactgcg cagggctgct tcccacagag
     1921 ctggccgacg aatttctgaa agaaattgaa aaagcaagca attataaagg ccagcctttt
     1981 tttaggctgt tacgcgcccg tattctcttt gagcacgggg ataccgcgga agcctttaaa
     2041 gaaacggaat atgtgcttac cttctcgcgt gcccataaaa accagctgcg ccttgtcgag
     2101 gcggggttgt taaaaatatt catgctgctg cgctgctccg aaacggcaag cgggcatcgt
     2161 gaaacaagca atctgttgcg tgaggctgtt tattacgcgc acaaagaccg tatcctcatg
     2221 ccgttctatc ttgaccgcgc cgtactgctg ccgctgctgc gtgggctgat cactcagtct
     2281 gcgggtaaaa gtttcatgag cgttgcggag ataaccttcg tgcgtgacgc gatagcgctg
     2341 tgtgacaata ctgtcaccgc ggcaagaaag ccggaaacgc tgtcggcacg cgagcttgaa
     2401 gtgctgggcg aattggcaaa gggtatcacg aatcgggaga ttgccgaaaa gctttgtatc
     2461 tcacaggcca ctgttaaaac ccatttgttg agtgttttcg gcaaactggg cgttacctca
     2521 cgggtgatgg cggtaaaggt gggccaaaag aaagggctta tactgtaacg gttagttcgt
     2581 aatataagac gaatcccagt gcaaacgaat cgtcacaaat ccttatacca ttcgggtttt
     2641 ttatagcatt tatccctaag cttaccgctt ggaaaattgt accccctgtt tacccaaaca
     2701 aatacgtctt acaaaaaaca aagagtttaa ataatactaa aatgcacatt gtaatttaga
     2761 ttatatgttt ttttataaag aagaaaataa ggacaggtaa aaatattaag acaaccgctg
     2821 ccacgctgcc tctccaagta tcaatttttt catgtgtgac ttgtaaattc atttttttat
     2881 cccaaagcgt ttttttatta gactttaaat aaataaacga aatgattgac gtaataatat
     2941 ttacatatgg caaacttaaa ccgtaaccgc aagaaaaaac cattagactg cgtaacaggg
     3001 agtttttaaa atttagtttg cttccatcac tgttacgaac agttatccta agaatccatt
     3061 tccccggtgt tgttccaatt gttgagataa gtattgcttc tatgattagc catactagtg
     3121 cggggatgct tatgtacgtt actgactgat ataaagcaaa aagaccgggc gtcataattt
     3181 tcaggaaaaa aataattatg gaatagataa gaacatagtc gatcattcgc gcaaaaaatc
     3241 gcacccatgg tctgacttgt ggaactttcc ctcgtttgta ttcttcgtca tgattcaatg
     3301 tttcttgtat tggtttttgc tttttttcgg cacggtattc caatatacca tgtgctaaaa
     3361 aataactttc atcttgagag cctatctgag taagctgctt atctcccttt acatctttta
     3421 caccattata atacttttcc atttccgaaa gctgctgtct ggattcacct tcataaaatc
     3481 cgttggttgt cattttaatc agcgagatgg cgatgatacg ttctattcca tatttcagaa
     3541 tctgttcttg ttttactttt gcatttaacc actcattcca agtcaacaaa tctgaaataa
     3601 ctaattcatt gctggatatg tcaatcactt caacattcag cttaccattt tttgattgta
     3661 gtacattcaa tccaaatgaa gtatccagat cggattcaat caggagaagt ttttcgaaaa
     3721 cctgtttata ctcttgaatt aacgactttt tctgtatata taatagtaac tcatcagata
     3781 cttcattaaa tgtgacttct ctcaagataa cgcgaaaagg gattggaatt ccgctatatg
     3841 tagaaaaaaa ggcctcaaac cgttgacata attcattttt ggcggcactc aatgcatgtt
     3901 cttcataagt ttcaggtgaa acataaagta tttttctaag cgcttgatcc gaatactgtg
     3961 aataaacatt cgtcaattcc tgtcccttat ccatcagatg gattcacccc gatattaata
     4021 atatatgtcc taaattttct atcaaattgg ctgttctgat gttgatttca taataccatt
     4081 attacccacc aattgcaata aaaccgtctt caatattgat cctatcatta atcacattca
     4141 gagacgatta ttgacattca tttaaacgga tgctataata agcgtgaatt aaagaacgga
     4201 aaaagcaaag gcgctatgca tcctgcaaag cgcctttgct ttttcgctga atgatatggg
     4261 gcagcaggac gggaaaacag tatcgcaagc gcaatgatca tggaacgccg cttgccgatc
     4321 gtgcagaaac ctcgttctgc gacgagatta agattgatga gacgaacggt ttgattaccc
     4381 gtatagaatg aggaggggta gcaatgacaa ccgagtatat cccggccgcg gagcaaaaga
     4441 ccgcaaaatg gtccggggga acgaccactg agctttacat ttatccgcgc accgccgact
     4501 ataaaaatcg cgattttctg ttccggctga gttccgccac tgtcgatgat gcggtttctt
     4561 ccttcacaaa gctggacggg tatgaccgca cgctgatcct tttgtcgggg agcctgcggc
     4621 ttgtgcacaa gaacatccgg gaggcaacgc tagggcagta tgacaaagac tgtttttacg
     4681 gcggttatga aacgacaggc tacggaagcg gcgtcacaga cttcaacctc atggccaaag
     4741 aaggctgccc ggcaaaagcg gagctgtgca gaatgcctgc tgtgggccgg atgcctgttt
     4801 ctgtggacag ggagacgcat gcgatcatcg gcttctatgc cctttcagga gacatccgga
     4861 taaactgtgg cggagagaaa tggaagcttg ccgacaaaga ttttttcaca gtcgaaaagg
     4921 aatcgggaga aaagcttgaa atcgaggcgc tttcgtttcc cggccggctt tttgtgttgt
     4981 cggtgctgac gataaacgaa acggctgtgt gattgccaac aattttgtct tgaaagataa
     5041 tgttgcggca gccgacgtat ctaaaactga ccaatgaggt ggatgaaatg gcgattgcgg
     5101 aaaacagcga ctttgaaatt tttacgcaga aactgattga aatttcaaaa gaatccccaa
     5161 cctatgaaaa gcttgcctat tatatcgaaa agaattacct caggctgatc tttatgacgg
     5221 cgggggagct cgcttccgaa ctgaacatca gtcaggggag tgtgtcacgt ttttttatca
     5281 ccctgggttt tcggggctac aacgactttc tccgcagcct gcaaaagatt gtcagcgcga
     5341 aaataaccgc gccccagcgg cttgaatata cggcggcggg cacaaaaatc gacagcacta
     5401 tcagcgtcga ggtcggtaat atgtgcgagc ttgaccggct catgcatgag ccggcttatc
     5461 ataagatgat cgactgcctg atttccgaca gggatctttt tatcctttct gcccgcatgt
     5521 ctgccacgct cgttccctac atgcgttaca ttctgtgcaa aatgcgggat tcagtgtacg
     5581 aagttacgcc ggattccatc gcatgggata cgatcaatct gcgtgagccg tcgaagacgg
     5641 agatttttac gatcgctttt ccgcggtatc caaagactct tgtcgacaaa atcaagaatc
     5701 tgcatgattc gggttttcat atttcagcga tcacagacag ccagctttcc ccgatcattc
     5761 catactgcga caatgtgatc gacgtaccca tcactgtttc ctcaattttt gacatataca
     5821 gtacgccaat ggcctttatt aacctcatgc ttcgggatgc cgcgaaagaa attccctcgc
     5881 tcgaagaccg cctcaaccgc atcgaaaaaa acgaagggga cagcggcgcc tattacaaaa
     5941 ggggaaaatg aagggtgaaa gaggaaatct ttcacaaatc ctttcgatgg aatattgaca
     6001 cgcatcgtga atgatgctat gatagaagca atgaagaatg atgatgaaat caatcaataa
     6061 caaacgatga tgtttgcatt aattgcagat gtcgtcattt ttttattatc gtttgcggaa
     6121 caaagttccg gcagaggtat tcgtcatctg gagtgccgga aatgatcata gcagaaatac
     6181 tatggtgtat cgcgccgtta atattgaaac gaaacatatt tgagagcaat ttttgttcgc
     6241 atagatttca ggaaaagagt gtgtacattg cccaatcata caataaccgt aaacaggcaa
     6301 agactgagtg ataacctttc ggcgctcgcg caaatcggca aaagcccgag tggagggctt
     6361 gaccgtcatt tcggaagcag cgcggatctt gaggcgagaa gatttttaaa agcgctgtgg
     6421 caaaaagctt tcggtgcgga ggtcataacc gacgcggccg ccaatctgtg ggtgaaagcc
     6481 ggcggtcttg aggctctgcc gcctatcgcg atcggatcgc atcacgacac cgtgaagaac
     6541 ggcggcatgt atgacggcgc aatgggtgtg ctgcttgcga ccgaggcttt tgaacgaatt
     6601 aacgaggcgg gtattcggct acgtcatccg ctgctgctcg ctgcctttac aggcgaggag
     6661 ccgaatccat tcggtttctc caccatcggc agtaagcttt tatccggcaa gcttacacac
     6721 gaaaaattga aaggcggtta tgacgccgaa gacggggtga caattgagga agcgctgaaa
     6781 aacgccgggg gtgattttgc ggccgttgat tcggtgcgga tgaatcccgg cgaactggcg
     6841 gcctttatcg agtgccatat cgagcagggc gtgatccttg aagggcgcgg cgctccgatc
     6901 ggtgtagttg aacggattgt gggcatatac cgcgaagagc ttttggtaaa aggcgaggca
     6961 aaccattccg gaacgacggt gatggcaagg cgccgcgatg cgctgacggc agccgcacag
     7021 gtcgtcttgg ccattgagga tgccgcccgt tcatttgaaa gtgataccct tgttgctact
     7081 gtcgggcgaa tggaggttct cccgaacagc gccaacatca ttcccggcga ggtcgcactc
     7141 acttttgaag ttcgcacgcc ggacgaaaac tgtaagaaaa aagcacttga gaagctcgga
     7201 aaagcgttta caatgatcat agaggagcgc ggcgtaacca tcgagcgcag aaccgcgctc
     7261 gaccagccca ctgcagtgat ggacgcccgt gtcgtttcgg cgttggaacg cgcttcgcag
     7321 gggatatgca agactctgcc gggcatggtc agcatggcgg ggcacgacgc ggcccatatg
     7381 gcaaaggtga cccgttccgg gatgctgttt gtccgtacgc cgggcggaaa aagccactgc
     7441 ccggaagaaa ccgcaaatct ggatgatatt gaaaaagcgg gcaacgccct gctttctgca
     7501 attattctat tggataagga gctggattga aacgaaaaaa ctgtatttgc ctgaacttct
     7561 ttttgacggc gaaaattttt tggcctcttc cggtgttgtc gaacaggacg gcatgatcgc
     7621 cgatatcgga gacgccgcgg cgttgaaaaa acaatatccg aacacagccg agatcaggtg
     7681 ggacggactt gttatggtgc ccggcactgt taacgtgcat aaccattcgt ttcagagcct
     7741 tttgcgcggg attgcggcgg ataggccttt tctcgaatgg cgggacgaag cgctttacaa
     7801 atattcgccg cggctgacgc cggaggacct ttacaacggc gcgttgttcg cattcggcga
     7861 aatgctgcgg tatggcgtca cgacggtctg cgattttttc tatcttcaca acgacggcct
     7921 tgaaagcgac gaggcgatca tccgcgcggc gcacgatctc ggtatccgcc ttgttctcgc
     7981 aagaacaatg tacgattggg aaggcgcgcc gaagggatat gtcgagtcgg ttgacaaagc
     8041 ctgtgaacat acaaaaatgc tttttgaaaa atatcggcac agcgatatgg tatcggtggt
     8101 tcccgcgccg cacagtctgc acgcggcatc gctggagatg atcaaagcgg gtcatgcgct
     8161 tgcaaaggag ctcggtacgg cctttcacat tcatgtggcg gaggagccgt tcgaggtcga
     8221 gcagatccgg aatcaatatg ggaaacggac ggtcgagctg cttgacgaca tcggcgtaat
     8281 tgatgaatcg acggtgatcg tgcatggcgt gtggcttgac ggcggcgaga ttgaaacgct
     8341 cgcgaagaag ggcggaaaac ttgcctactg cccgtcgagc aatatgtttc tggcggacgg
     8401 aatcaccgac atcgaaaaaa tgctgtcgct cggcgtaaaa atcggacttg gaaccgacgg
     8461 aggatgcagc aacaaccgca tcagcgtttt tgaggaaatg cgcatggctc cgctgctgca
     8521 aaaggcgtac aaacacaacg cactttgcgt taattaccga gaggcattta aaatgggcac
     8581 ggaatggggc ggcgagctgc tcggcttacc ggtcggaata ctgaaaaagg gctattgtgc
     8641 tgattttgtc ggcattgacc gttacgacct ttccatgcag ccggtaacgg agcgcggcgg
     8701 gcagctgctt cccaatattg tctattccat gcagccgggt gcgatcagac gcgtcgttgc
     8761 gggcggcgtt gaacgtgcgg cggacgggca cctttcgagc attcctgaaa gaacggtgat
     8821 tgagcgcgta aaagagacaa tgagccgtat cgaacggtaa tcctccgtat ccctgatgat
     8881 ggatcgcaga gttgatgaac agcgaaaatc tgaatcttga cgatagatcg aaaaacatta
     8941 aagggggcaa aagccatgaa tttcaatgag caggcaaaag aagcgatgca gatcatgctt
     9001 gacgacggac ttccggaaat gccggagttt gcggatggaa tccgccgggc accgtcacgc
     9061 ggatttcatc tgacaaagga gcaaaccaaa acggctctga aaaacgcgct gcgctatatt
     9121 cagccgaagt atcacaagga gcttattccg gaatttctga acgagcttat gacctacgga
     9181 agaatctacg gctatcgctt tcgcccgcag ggcagaatct acggcaggcc gatcggtgag
     9241 tatcggggca actgcattga aggcaaggct tttcaggtga tgatcgacaa caacctcgat
     9301 tttgaagtcg cactttaccc ttacgagctt gtcacctatg gcgaaaccgg cagtgtctgc
     9361 cagaactgga tgcagtacag gctgatcaaa aaatacctcg aggtattgac gcaggatcag
     9421 acgctggtcg tcgaatccgg gcatccgctc ggccttttca aatcaaagcc gacggcgccg
     9481 cgcgtcatca tgacaaacgc gctgatgatc ggcgcgttcg acaatgaggc cgactggtcc
     9541 gtagccgaag agatgggcgt cgcgaattac gggcagatga cggcaggcgg ctggatgtat
     9601 atcggcccgc agggcattgt gcacggaacc tataacaccc ttctgaacgc ggggcgctta
     9661 aagctcggca taccgaacga cggagacctt gccggcaggc tgttcatcag ctctgggctc
     9721 ggcggcatga gcggagcgca gtcaaaggcg atcgagattg caaacggcgt cggtatcatc
     9781 gccgaggtcg acgggtcaag aattgcaaca agacacgatc agggctgggt cggggtggtt
     9841 tccgacaatc ttgacgaggt ctttcggatc gccaaagaac atcttgaagt acaaaagagc
     9901 gtttccatcg cttatcacgg caatatcgtc gatttgcttg aatatgttgt caaaaataaa
     9961 ataaaggtcg accttttgtc cgatcagacc tcctgtcacg ccgcttacga gggtggttac
    10021 tgcccccagg gagtgacctt cggtcagcgc acggaattgc tcgcgaccga ccgcgacaag
    10081 ttctgcggcc ttgtcgataa aagccttcga cgccactatg aactgatcaa gtcgtgcgtt
    10141 gcaaacggca cctacttctt tgattacgga aattccttcc tcaaggccat ctacgacgca
    10201 ggcgtaaagg aaatttcaaa aaacggcatt gacgacaagg acggttttat cttcccgtcc
    10261 tacgttgagg atatcatggg cccgcagctg ttcgattacg gttacggccc gttccgctgg
    10321 gtctgcctga gcggtaccga cggggatctt gaaacaacgg acaaagctgc gatgagctgc
    10381 atcaatgtag aaggccgcta tcaggacaga gataattata attggattcg cgacgcaaaa
    10441 aagaaccggc tcgtcgtcgg taccaaggcg cgcattttgt atcaggacgc ggaaggacgc
    10501 atgaatattg ctctgaagtt taacgaaatg gtacggaacg gtgaaatcgg ccccgtgatg
    10561 atcggccgcg atcatcacga cgtcagcggc actgactctc ctttccgcga gacggcgaat
    10621 atccgggacg gcagcaatgt gatggcggac atggcggttc aatgctatgc gggcaacgcc
    10681 gcacgcggta tgtcgctcgt gacattgcac aacggtggcg gcgtcggcat tggaaaatcg
    10741 attaacggcg gctttggttt ggtactcgac ggcagcgagc ggatcgacga gattatcaaa
    10801 tgttcgattt tatgggacgt gatgggcggc gtcgcccggc gcagctgggc gcgtaacgag
    10861 cattccatcg aaacgtcgat tgaatataac aggaaaaacg cagggcaaag ccagatcacc
    10921 cttcccttta ttccggatga ggagcttgtc gcaaaaatcg tcgaagcggc attgaaaaag
    10981 tgaaatggga aatatgctga tcctgaacgc aggggaaatc gcgacaccga tcggcagaac
    11041 ggcgctgcgc ggtgcgcaga tgaatgcaat caaaacaatc cccaacggtt cggttgccgt
    11101 gataaacggg aaaattgcag ccgttggccc gactgatgag atcgagcggg aatacgggcg
    11161 gaaggggctg ccggttattg acgcggccgg atgctgtgtt gttccgggct ttgtcgattc
    11221 gcacacccat tttattttca gcggataccg cgaagaagaa tttttaatgc gccttcaaaa
    11281 atgcagttac cttgatatta tgcgcgcggg cggcggcatc gtgaataccg taacgcatac
    11341 aagggagctg tcacacgatg gattgtatca actggggaag gaacgtctgc acgaaatgct
    11401 tgcctttggc gttacaacgc ttgagggcaa aagcggctat ggtcttgacc ttgaaagcga
    11461 gctcaaaatg ctcagagtgc aaaaacagct tgacgcggac ggcccgtgcg acatcgtggc
    11521 gagcttcctc ggggcgcacg ccgtcgcgcc tgagtttaag ggccgcgcgg acgattttat
    11581 aaatttcctg atcgaatcgg tgctgcctgt tgtgacgagg gagaagcttg cagagttttg
    11641 cgatgtgttc tgcgagacgg atgttttttc agtggaacag tcacagaagc tgttgcttgc
    11701 cgcgaaaaaa atgggcctta cgccgaaggt tcacgccgat gaaatgtcga atctcggcgg
    11761 cgcgcagctt gcgtgcaggg tgaacgccat atcagccgat catctgctca acgtctcaaa
    11821 agaaggaata caggcactcg ctgaaagtga gacggttgca acgctgctgc ccgcgacggc
    11881 tttttgcctt ggcatgccat acgcggtcgc gcgcggcatg atcgactcca gctgcgccgt
    11941 cgctcttgcg agcgattata atccgggcag ctgttttaca aattccgttc cgcttattct
    12001 ttcgctggcc tgtatttata tgggcatgac ggcggcggag gcgctgaccg ccatgacaat
    12061 caacggggct gccgcgatcg gcagggcgga taaaatcggg agcatcgaac cgggcaaaca
    12121 ggctgacctg ttgattctca gatatccaag ttataaattt ctcatataca atacggcggt
    12181 caacatcgtc ggcaccgtga tcaaaaacgg gcgggtcgca tatcaaaatt tataagactg
    12241 tggaagtgaa atcatggtaa tattgaccgg caacacattg acactcgaag aaattgatcg
    12301 aatcatcaac agagatgaga aggtacaggc cgcgccggaa agcatggaaa aggcggcggc
    12361 ttcaagaaga gccgtggaac gggtcgtgag tgaaaacaga accgtttacg gcattacgac
    12421 cggtttcgga aaattcagca gcgtgcgcat tgatccggag gattccgacg agctgcagct
    12481 taatctcatt tattcgcatg cctgcggcgt tggcgaaccg ttcagcccgc aggttgcaaa
    12541 aacgatgctg ctccttcggg taaacacgct gctgaagggc tactccggcg tcagtctgcc
    12601 ggtcattgac ctgatgatcg agcttgtcaa ccgcaacatc acacctgtcg tgg
//
LOCUS       DATALY010000013         5192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_693782,
            whole genome shotgun sequence.
ACCESSION   DATALY010000013 DATALY010000000
VERSION     DATALY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693782"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VHR42_00430"
     CDS             <1..1237
                     /locus_tag="VHR42_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3025686.1"
                     /translation="MVVVGRFSGSDALAAVGATGSIINLIVNLFMGLSVGTSVVVARD
                     YGAGKEDDVSRSVHTSIAISIIGGLMVMALGLAFSKPLLVIMGTPADILGLSVLYLKI
                     YFISMPANMVFNFGAAILRAVGDSRRPMYYLVVTGILHVIFNLFFVIVLHMSVDGVAW
                     ATVISQYLSVLLILLCLSRYDGAIRFIPRQMCIDRQKLKAIVQIGLPAGLQSLLFSIS
                     NVLIQSAVNSFGSIMVAASSAANNVENYVGTTMNAYYNSAITVTAQNMGAKKYDRIDT
                     IAKVCTVLIFSTWILLGGATLLFGRPLLGIFTTDPNVIELGMLRLVVMMAAYFTCGTM
                     NVYPGLSRGMGYSILPMLCTLVGACLLRIVWLATIFTWYPTEIVLFACYPVTWALAGI
                     GQVIIFFYARRQIHKQILA"
     gene            complement(1395..2516)
                     /locus_tag="VHR42_00435"
     CDS             complement(1395..2516)
                     /locus_tag="VHR42_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3025687.1"
                     /translation="MKHLMKFFCLILAAALLVSVAGCSKSAGKSSASAGTITITDHNG
                     NTVKLPSHINRIVVCDILPLPSVLAVFFDSAKKIVGMSEPSMTAAKNGLLKQLYPEIL
                     KAKTDFIKDSDVNVEELAALKPDVVFYSASSKGLGEKLKKSGFNAVAISVNKWGYNSI
                     ETLNNWIALLSKIFPDNDKTKIVADYSNKTYDMVHKRVANLSDSERERIFFLFKYSPN
                     SIQTSGKLFFGEWWAEAVGAVNVSNELSKDNSVAVNMEQIYKWNPQKIFITNFTTAQP
                     KDLSGNTVGSFNWSGIKAVQSKQVYKMPLGMYRSYTPGVDTPITLMWLAKTVYPKLFE
                     DIDITRETKKYYKTVFGIDLTDQQANSIFTPTSAAAKGF"
     gene            complement(2533..3672)
                     /locus_tag="VHR42_00440"
     CDS             complement(2533..3672)
                     /locus_tag="VHR42_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX3025688.1"
                     /translation="MRQTASRISIYAADVSGVCSALFELGGMTVMHDASGCNSTYNTH
                     DEPRWYDMDSMVYISGLSEMEAIMGDDEKLIDDIVSAAKQLSPRFIAIAGTPIPMMIG
                     TDFQAIAAVVERRTGIPVFGFDTNGMNSYLSGVSMAFQTIASRVVDRDAHKGSNRSVN
                     IIGATPLDFSVNGSVKSIENFLTDNGFIIISNWAMGGTLDKIRNAGRAAVNLVVSYGG
                     LSAAKELQKIFGTPYVIGVPYGKNFSDKLLDDLKEAADIGRSKVSFTGRKISNMPDIT
                     VIGESVGSGSLAAAISAESGKTVQVLCPLETEARLLADGDFIAADEDDLIPLLKKSKV
                     LIADPLYRPICPESCKFIPLPHEAFSGRIYRNEIPDFVARTIKLY"
     gene            complement(3669..4997)
                     /locus_tag="VHR42_00445"
     CDS             complement(3669..4997)
                     /locus_tag="VHR42_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX3025689.1"
                     /translation="MLKSISRIRDEYGASVLIKDASFSSPFVSGLEFGSPARGVWNIV
                     HVGMLIPEAHEIFVCASSCLRGVVLTAAEMNASDRFSCITIGESNVLEGDMESLIIDG
                     VTDILNKLPKRPPAVLIYTSCIHHFMGCDLPLVYRTLGEKFPDVAFTDCYMNPIMRKS
                     GLTPDQIMRRQLYSLLEKRSLNPVSVNIIGNNAPTDESSELVRMLRSNGFEIKDITRC
                     RSYRDYLDMAGSAVNISYNPAAKAGGDALEKRLGQRHLYLPLCYGYGEIEKNLDLLAD
                     YLKIRKPDFTSAVERAEQSLKKAKEVIGDTPIAVDYTATMRPLGLARLLMEHGFHVER
                     IYADGFNKEEIDDFEWLKENRPDLKLYATVQVKMRVLPRKCDEKTLAIGQKAAYFTGS
                     DYFVNIVEGGGMYGFDGICRMAELMTDAFLQKKDARSLIQIKGLGCGCCL"
     gene            complement(4991..>5192)
                     /locus_tag="VHR42_00450"
     CDS             complement(4991..>5192)
                     /locus_tag="VHR42_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HEX3025690.1"
                     /translation="EDAKVEELAGDIHSSIVGTLDRSETVQAAEELQKTVLEAFPESE
                     MARQYRKLAVSVMHVCGKEIIC"
BASE COUNT         1173 a         1383 c         1239 g         1397 t
ORIGIN      
        1 tatggttgtc gtcggacgtt tttcaggcag cgacgcgctg gccgcggtgg gcgcgacagg
       61 ttccatcatt aacctcatcg tcaacttgtt tatgggactg tcggtaggta cgagtgtcgt
      121 cgtggcacgg gattatggtg ctggaaaaga agacgatgtt agccggtccg ttcacacttc
      181 catcgctatc agtatcatcg gcgggctgat ggtcatggcc ttaggcctcg cctttagtaa
      241 gcctttgctt gtgattatgg gcaccccggc ggacatttta ggcctttcgg tattgtattt
      301 gaaaatctac tttatcagca tgcctgccaa catggtgttc aactttggcg cggcgatcct
      361 acgcgctgta ggcgacagtc gccgcccgat gtattacctt gtcgttaccg gtattctgca
      421 cgtgatattt aacctgttct ttgtgatcgt tttgcacatg agcgtcgacg gtgtggcatg
      481 ggccacggtc atttctcagt atctctcggt gctgctgatt ctgctttgcc tatccaggta
      541 cgacggagcc atacgcttca taccccggca gatgtgtatt gatcggcaaa agttgaaggc
      601 cattgtgcag attggcttgc cagcaggcct gcaaagcctg ctgttctcca tttccaatgt
      661 gctgattcag tcagccgtta attccttcgg ctcaatcatg gtagcggcca gttccgctgc
      721 aaacaatgtt gaaaactatg tcggcaccac aatgaatgcc tattacaact cggccataac
      781 cgttaccgca cagaatatgg gtgcgaaaaa gtatgatcgt atcgacacca ttgcaaaggt
      841 gtgcacagta ttgattttct ccacctggat tctcctgggc ggcgcgacat tgctgtttgg
      901 caggcctttg ctcggtatct tcacgaccga tccgaatgtg attgaattgg gcatgttacg
      961 tttggttgtc atgatggccg cttacttcac ctgcggcaca atgaacgttt atcccggcct
     1021 gtcccgtggg atgggctatt ccatattgcc tatgctctgc acgctggtgg gcgcctgcct
     1081 cctgcgaatt gtctggctgg ctacgatctt tacctggtat cctacagaga ttgtgctatt
     1141 cgcatgctat ccggtcacct gggcgctggc aggaatcggt caggtaatca tctttttcta
     1201 cgcacggcgc caaatacata agcaaatttt ggcttgatca cttatttcga acttcatatg
     1261 tttgagatac tttttacagc aggccgtttt aacatctttc gattccaccg taaaaaagaa
     1321 aaagcacccg accccaaaaa gccaggtgct cagattgaca tcattcagct gcaagccgcc
     1381 ggcgtcgttc ccaattaaaa cccttttgcg gcagcggatg tcggggtaaa aattgaattt
     1441 gcctgttggt cggtcagatc aatgccaaat acggtcttat aatatttctt tgtttccctg
     1501 gtaatatcaa tatcctcaaa aagcttggga taaaccgtct ttgccagcca catgagcgtg
     1561 atcggcgtgt cgacaccggg cgtatagctg cggtacatgc caagcggcat cttatagacc
     1621 tgcttgcttt gaactgcttt gataccactc cagttaaagc tcccgaccgt gttgccggac
     1681 agatccttcg gctgcgcagt ggtgaaattg gtgatgaata ttttctgagg attccactta
     1741 taaatctgct ccatattgac cgcgacggaa ttatctttgc taagctcatt ggaaacattc
     1801 accgccccga ccgcctcggc ccaccattcg ccgaaaaaca gttttccgga ggtttgtatg
     1861 gaattcgggc tgtatttgaa caggaaaaag atgcgctcgc gttcgctgtc ggaaaggttt
     1921 gcaacgcgct tatgcaccat atcgtacgtt ttgttgctgt agtccgcgac aattttcgtc
     1981 ttgtcgttat ccgggaaaat tttactcaga agggcgatcc agttattaag cgtttcgatc
     2041 gagttgtatc cccatttatt aacggagatc gcaacggcgt taaatccgga ctttttcagt
     2101 ttttcgccaa gtcccttgct ggaggcactg taaaaaacaa catccggttt cagcgctgca
     2161 agctcttcca cattgacgtc gctgtctttt ataaagtcgg ttttggcttt caatatttca
     2221 ggatacagct gcttgagcaa gccgttcttc gctgcggtca tgcttggctc cgacatgccg
     2281 acgatttttt ttgcggaatc aaagaaaacc gcaaggacgg aaggaagcgg gagaatatcg
     2341 cagacgacga tacggttgat atgagagggc aactttaccg tattgccgtt atgatccgta
     2401 attgtgattg ttccggcgga agcgctgctt ttgcctgcgc ttttggaaca gccggctacc
     2461 gataccagca gcgccgcggc cagtatcaag caaaaaaatt tcatcagatg tttcatttca
     2521 ttttacctct tctcaatata acttgatcgt ccgggcgaca aaatcaggaa tttcattgcg
     2581 gtatatcctg cctgaaaacg cctcatgcgg cagcgggata aacttgcagc tttccgggca
     2641 tatgggacga taaagcgggt cggcgatcaa aacctttgat ttcttcagaa gaggtatcag
     2701 atcgtcctcg tccgcggcga tgaagtcgcc gtccgccaaa agccgggctt ccgtttccag
     2761 cgggcaaagc acctgcaccg tcttgccgga ttcggcgctg attgccgccg cgagggaacc
     2821 ggatccgacg ctttccccga tgaccgtaat atccggcata ttcgaaattt ttctgccggt
     2881 gaaggatacc ttgcttctcc cgatatcggc ggcttctttt aaatcatcca gaagcttatc
     2941 cgaaaagttt ttgccgtatg gcaccccgat cacatagggg gtaccgaata ttttttgaag
     3001 ttctttcgcc gccgagagcc cgccgtaaga caccacaaga ttgaccgcag cccgccccgc
     3061 attacggatt ttatccagcg tgccgcccat cgcccagtta ctgatgatta taaaaccatt
     3121 atccgtcaga aaattttcga tcgacttcac acttccgttt acggaaaagt caagcggagt
     3181 cgcgccgatg atattgacag accggttgct ccctttatgg gcatccctgt caaccacgcg
     3241 gcttgcaatc gtctgaaagg ccatactgac gccggataag taggaattca ttccgtttgt
     3301 gtcaaagccg aataccggta ttccggtccg gcgctccact acggcggcaa tcgcctgaaa
     3361 atctgttccg atcatcatcg gaatcggggt tccggcaatc gcaataaacc gcggcgacaa
     3421 ttgtttggcc gcacttacaa tatcatcaat cagtttctca tcgtcgccca tgatcgcttc
     3481 catttccgag agcccggaga tatagaccat gctgtccata tcgtaccacc ggggttcatc
     3541 gtgcgtgtta taggtcgaat tgcatccgga agcgtcatgc atgaccgtca tgccgcccaa
     3601 ctcgaagagc gccgagcaca ctcccgacac gtcggccgca taaatggata tcctgctcgc
     3661 agtttgtctc ataggcagca tccacacccc aatcctttga tctgtatcaa gctccgcgca
     3721 tcctttttct gcaggaaagc gtctgtcatc agttcggcca tgcggcaaat accgtcaaat
     3781 ccgtacatgc ctccgccctc aacgatattc acgaagtagt cggaaccggt aaaatacgcc
     3841 gccttttgtc cgatcgccag tgttttttcg tcgcattttc ttggcagaac acgcattttc
     3901 acctgaacgg tggcgtataa cttcaaatcg ggcctgtttt ccttcagcca ctcaaaatcg
     3961 tctatttctt ccttgttaaa tccgtcggcg taaattcttt ccacatggaa cccgtgctcc
     4021 ataagaaggc gcgcaagccc gagcggacgc attgtcgccg tataatcgac tgcaatcggc
     4081 gtatcgccga ttacttcttt tgcttttttc agcgattgct ccgcgcgttc aacggcagat
     4141 gtaaagtccg gtttccggat tttcagatag tccgccagca gatccagatt tttttcaatt
     4201 tcaccataac catagcagag aggcaggtac aaatgtctct gcccaagacg cttttccagc
     4261 gcatcgccgc ccgctttcgc cgcgggatta taagaaatat tcactgcgct ccccgccatg
     4321 tcgaggtaat cccggtaaga tctgcaccgc gtgatgtctt tgatctcaaa accattgctg
     4381 cgaagcatac gcacaagctc gctgctttcg tcggtcggcg cattgttgcc gatgatattg
     4441 acggagacgg ggttcagaga gcgtttttca agcaggctat acagctgccg gcgcatgatc
     4501 tgatccggcg tcagcccgct tttgcgcatg atcggattca tataacagtc tgtaaacgcg
     4561 acatcgggga atttttcccc cagcgtgcgg taaacgagag gaaggtcgca tcccatgaag
     4621 tggtgtatac agctcgtgta tatcagcacc gccggaggcc ttttcggcaa tttattcaga
     4681 atatccgtta caccgtcaat aatcaggctt tccatatcgc cttcgagtac gttgctctca
     4741 cctatcgtaa tacaggaaaa acggtcggat gcgttcattt cggccgccgt cagcactacg
     4801 cctcggaggc agcttgaagc acaaacgaaa atttcatgcg cttcagggat cagcatgcca
     4861 acatggacaa tattccagac gcctctggcg ggcgagccaa attccaaccc cgatacgaag
     4921 ggcgaggaaa acgacgcgtc ctttatcagc acgctggctc cgtattcatc ccggattcgg
     4981 gaaatcgatt tcagcatata atttcctttc cgcagacatg catgacactt acggccagtt
     5041 tcctgtactg ccttgccatt tcactttccg gaaacgcctc aagaactgtt ttctgcagct
     5101 cttccgccgc ctgaacagtc tcgctgcggt caagcgttcc gacaattgag gaatgaatgt
     5161 cgccggccag ttcctctact tttgcatctt ca
//
LOCUS       DATALY010000014         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_694044,
            whole genome shotgun sequence.
ACCESSION   DATALY010000014 DATALY010000000
VERSION     DATALY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694044"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1139
                     /gene="prmA"
                     /locus_tag="VHR42_00455"
     CDS             222..1139
                     /gene="prmA"
                     /locus_tag="VHR42_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3025691.1"
                     /translation="MDWTEITVEVNAEDIDRAGDIAQMVVPYGIYIEDYSHLNEEVEE
                     IAHIDLIDEELLKKDRTKAKVHVYISPEESPAEAIAFLSERYNAVGIRHAIRMDPCVE
                     EDWINNWKKYFKPIKVGQKLLIRPTWEEEYEAGNRKVLHLEPGLAFGTGTHETTRLCM
                     ELLEEYVRPGFDVLDVGCVSGILSVAALLLGAEKAVGVDIDALAVKTAVQNAEINHVE
                     DRFTGICGDLTEKVSGKYQIVVANIVADVIIELTKNVGQFMYPDTVYLMSGIIDTREK
                     DVLTALESRFEILSRKTEKGWVALAAKQK"
     gene            1348..2082
                     /locus_tag="VHR42_00460"
     CDS             1348..2082
                     /locus_tag="VHR42_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX3025692.1"
                     /translation="MKKGCWLLFGTAVLLLVIGANSLANFFGGYVYYLFYQGIYGIVL
                     STLLPVFYVFFVEKGGLNELGIRKITIRSVLVAVLFIAFSIGGQLYKKGFIISSSDYL
                     IMISIPLVMTTFFEEFLFRGFFQIRFEKIFGTVPAVLLSGLMFSLYHLGYPKFRSWDS
                     LLTLLLVGIMFALSFKLSGNSLFTAYFVNLPNAILTYLYNPHNFPDFNLCTAAVSAAV
                     ILLLIFFFVLAPGRLKQAEQRIPTAI"
     gene            2095..>2281
                     /locus_tag="VHR42_00465"
     CDS             2095..>2281
                     /locus_tag="VHR42_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3025693.1"
                     /translation="MNITVRPAQPSDAPEISRIYAASWRRAYRGMVPQEYLDQLKDKN
                     DHWTGKFCRDLGQGTLHA"
BASE COUNT          582 a          490 c          618 g          591 t
ORIGIN      
        1 tgaatatcag ggtgcggaac cagatattgc tcagccgcag cagaacggag ccggcggtta
       61 aaataaccgc gttgagaatg aatttatttt ttttcatttg attaacacct ttatctgttg
      121 ttaatcaaaa tgtattcaca aggggcgtaa aataaatcta tattttttcg gccttatcgc
      181 gtataataaa agattagaaa atgaaattac ggaggaagca tatggattgg acggaaatca
      241 ccgttgaggt aaacgcagag gatattgacc gtgccggcga catcgcgcag atggttgtac
      301 cgtacggtat ttatattgag gattactccc acctgaacga agaggtagag gaaatcgccc
      361 atattgatct gattgacgag gaactgctga aaaaagatcg cacaaaggca aaggtgcacg
      421 tttacatcag cccggaggag agccccgcag aggcgattgc gtttttgagc gagcgctata
      481 acgccgtcgg catccggcat gcgatccgca tggatccctg cgtggaggag gactggatca
      541 acaactggaa aaagtatttt aagccgatca aggttgggca gaagcttctc atccgcccca
      601 cgtgggagga ggaatacgag gccggaaatc gcaaagtgct ccatctggag cctgggctgg
      661 ctttcggcac gggcacgcac gaaaccacgc gtctctgcat ggagcttttg gaagagtatg
      721 tgcgaccggg atttgacgtg ctggacgtgg gctgtgtcag cggaattctt tccgttgcgg
      781 cgctgcttct gggtgcggaa aaggcggtgg gcgtggatat cgacgcgctt gcggtgaaaa
      841 ccgcggtgca gaacgcggag atcaaccatg tggaagaccg cttcaccggc atctgcggcg
      901 acttgaccga aaaggtcagc ggaaaatacc agattgtcgt cgccaatatc gtcgccgacg
      961 taattattga gctgacaaaa aatgtagggc agtttatgta tccagatacg gtttacctga
     1021 tgagcggcat catcgatacg cgagaaaagg atgtgctgac cgcgctggaa agccgatttg
     1081 agattctttc acgtaaaacg gaaaaaggct gggttgctct ggcggcaaaa caaaaataaa
     1141 gaggtaagaa ttatggcact ggaaaacgga tcggaaacct gtacctgccc aaagagtaac
     1201 tgtgagcgcc acgggaaatg cgcggagtgc ctcgagcatc ataagaacca taaaaggttc
     1261 cctccctact gcaaacggga aaggaaaccg cgttcggaaa gacgggcacg ccgccatgaa
     1321 tgaggctttt gaaaaaataa aggcactgtg aaaaagggat gctggctgct ttttggtaca
     1381 gccgtacttt tgctggtgat tggagcaaac agtctggcga actttttcgg aggatatgtt
     1441 tattatctgt tttatcaggg aatctacgga atcgttttaa gcacgctgct gccggttttt
     1501 tatgtgttct ttgttgaaaa gggcgggcta aacgaactgg gtatccgtaa aatcacgata
     1561 aggtccgttc tcgtagcggt tttgtttatc gctttttcca tcggcggcca gctgtataaa
     1621 aagggtttta taatctcgtc ttccgattac ctgattatga tttcgatccc actggttatg
     1681 accacgtttt ttgaagaatt cctgttccgg ggctttttcc agatccggtt tgaaaagatc
     1741 ttcggaacgg tgccagccgt tctgctttcc gggctgatgt tttcgctgta tcaccttggt
     1801 tacccgaaat tccggtcgtg ggattcgctt ttgacgctgc ttttggtcgg catcatgttt
     1861 gcactgtcct ttaaactttc cggaaattct ctgtttacgg cctatttcgt gaatcttccg
     1921 aatgcaatct tgacctattt atataacccg cataattttc cggattttaa tctgtgtacc
     1981 gctgcggttt cagcagccgt tattcttctt ttaatctttt tctttgtgct ggctcccggc
     2041 agattgaaac aggcggagca gcgtattcca acggcaattt aaggagagaa tgttatgaat
     2101 attacagtca ggccggcgca gccgtcggat gctccggaaa tcagccgtat ttatgccgca
     2161 agctggcgga gagcctaccg cggaatggtt ccgcaggagt atttggatca actgaaggat
     2221 aaaaatgacc attggacagg gaaattctgc cgtgatctgg ggcagggcac cctgcatgcc
     2281 t
//
LOCUS       DATALY010000015         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_2426,
            whole genome shotgun sequence.
ACCESSION   DATALY010000015 DATALY010000000
VERSION     DATALY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VHR42_00470"
     CDS             complement(<1..773)
                     /locus_tag="VHR42_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007727126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3025694.1"
                     /translation="MNIAGKKVLVTGADGFIGSHLAEELVRRGCSVRAFVCYNSFNSW
                     GWLDQSPKEIQSELDIFQGDIRDPYRVKTALEGCSAVFHLAALIAIPYSYYSPCSYID
                     TNIKGTLHVLQAAQELGTERVVQTSTSEVYGTAAYVPIPETHPLQAQSPYAASKIGAD
                     QLALSFYRSFSTPVSVIRPFNAYGPRQSNRAVIPTVITQIAQGKRRIKLGSLTPTRDF
                     SFVRDTVRGFWETACCDGAVGEVVNIGSGFEVSIGETVQ"
     gene            complement(969..>2013)
                     /locus_tag="VHR42_00475"
     CDS             complement(969..>2013)
                     /locus_tag="VHR42_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3025695.1"
                     /translation="VLSVCMTLGYQLVERLFDFSGGSGHFSPQWWNFLLPSAWFAAPF
                     SLLVDHDYSTIYLLLTAAGIVIPILAVILYLKAVAPAFEKNLQKLAGSAERGHTEKNL
                     SVQHTVSRLLCPGKLEQVFFIFSSQIMQNERKLKLRLYPNIAFAVIFPFIFLFNRINE
                     RQSFAQTLAQIKSGNFFLFLYLSAMMLAPMLTILGMSENYKGAWIYRMMPVASPEEIL
                     RGTFTAFICKYVFPVFLFCGLVFALIYGPKIIPDLILIFLNTLLLMLLVFKYSDKELP
                     FCKDFQATQNNNISILILSLVLCGVFAGLHYLLRRFFSPGIFLFLAVSLVFSLILWRT
                     AFHVTWQELAENE"
BASE COUNT          563 a          481 c          508 g          461 t
ORIGIN      
        1 atctgaacgg tttcaccgat tgaaacttca aatccgcttc caatattgac gacttcgcct
       61 accgctccgt cgcagcatgc cgtttcccaa aaaccccgga ccgtatcccg cacaaagctg
      121 aagtcccggg taggggtaag agaaccgagt ttaattcggc gtttcccttg cgcaatctga
      181 gtgattacag tgggaatcac cgcccggttt gactgccgcg gcccataggc gttaaatgga
      241 cgaatcacgg aaaccggcgt ggaaaacgag cggtaaaagg agagcgcaag ctgatcagcg
      301 ccgatttttg acgcggcata aggagactgt gcctgcagcg gatgtgtctc aggaatgggg
      361 acgtacgccg ccgttccgta cacctcgctg gtagaagtct gtacaacgcg ctccgttcca
      421 agttcctgcg ctgcttgcaa aacatgaagt gtccctttga tattcgtgtc aatataggag
      481 cacggggaat aatacgaata gggtatggcg atcagggctg ccagatgaaa tacggcgctg
      541 cagccctcta gagcggtttt tacgcggtag ggatcgcgga tgtcaccttg aaaaatatcc
      601 aattccgatt gaatctcttt gggagattga tccagccatc cccatgaatt gaacgaattg
      661 taacagacaa aagcgcgtac ggagcatccc cttcggacca gttcctccgc taaatgagaa
      721 ccgataaagc cgtccgcgcc cgtgaccaga accttttttc ctgcgatatt cattttgtgc
      781 ccccaatgat gaagtattct ctgaaagaaa gccggttttc gcttctttcc attcatattc
      841 cggctgatta aaaaatagaa gcgcctgtgt cttcaggcgc ttccaaacat gaaattgctt
      901 catcggaggg attgcgatcg cgtgataaac ctctgtaaat ccgttcagtc gtgcgggttg
      961 caggtgcttc actcgttttc tgcgagttcc tgccatgtga catggaaagc agtacgccat
     1021 aaaatcaagc taaacacaag cgacaccgca aggaagagaa agattccggg actgaaaaag
     1081 cgcctcagca gatagtgcag gcccgcaaac actccgcaca agaccaaaga aagaatcaag
     1141 atggaaatgt tgttgttctg cgtagcttga aaatccttgc agaaaggaag ctctttgtct
     1201 gagtatttaa agacaagcag catcagaagc agggtgttga gaaaaatcaa aataagatcg
     1261 ggaataattt tgggtccgta gattaatgca aaaacaagac cgcaaaaaag aaagacaggg
     1321 aacacatatt tgcaaatgaa agccgtgaac gtcccccgga gtatttcttc ggggctggcg
     1381 acgggcatca tcctgtaaat ccatgcgcct ttgtagtttt cgctcattcc aagtattgtg
     1441 agcataggcg cgagcatcat cgccgacagg tataaaaaca aaaagaaatt accgcttttt
     1501 atctgtgcca gagtttgcgc gaacgattgc ctttcattta tacgattgaa aagaaagatg
     1561 aatggaaaga tcaccgcaaa cgcgatgttt ggatagagcc tgagttttag cttccgctca
     1621 ttttgcatta tttgcgagga gaaaataaaa aatacctgtt ccagtttgcc ggggcagagg
     1681 aggcgtgaaa ccgtgtgctg taccgagaga tttttttccg tgtgtccccg ctccgcactg
     1741 ccggcaagtt tttgaaggtt cttttcaaag gcaggcgcca cggcttttag gtagaggata
     1801 accgccagaa tcggaatgac gatccccgcc gctgtgagta gcaagtaaat cgtgctgtaa
     1861 tcgtgatcca ccaacagact gaagggcgcg gcaaaccacg ccgatggcag cagaaaattc
     1921 caccactgtg gggagaaatg tccgctcccg ccggaaaaat caaacagccg ctctaccaat
     1981 tggtagccca gagtcatgca gacggaaagg acg
//
LOCUS       DATALY010000016         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_435764,
            whole genome shotgun sequence.
ACCESSION   DATALY010000016 DATALY010000000
VERSION     DATALY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435764"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2511
                     /locus_tag="VHR42_00480"
     CDS             190..2511
                     /locus_tag="VHR42_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3025696.1"
                     /translation="MKNKTWIPALPVIAVSFLLYLIVSTLQNEFFGDILSPFCALLAS
                     AIIFNTYFKSGRARKINRIWAALGMACLSWAAADILWALFDFVFHLDPQESVLISILY
                     MGTNFFLFAAIFIYSVYKFRKWNAIQLVVDAVMISVTSIFLLWILFFDKDPEMFSRIG
                     NEGWITSVSIIIDVAAFICIVVWCFSIHGGKLPRYLYVSIGSIFLYIVSDLYWFYLDI
                     NNTYAPNSLIDAAYMASLLGIAVAAEMFRADSGAGERDFSNVGSKNRGLFLLSGLLLT
                     VVFKGFVLTDLLVFLFITLAYISFTVYIQGSIKSDQLLRKEYDLNAELESRIADRTKE
                     LDYLSNRDLVTNLYNRRSFIRRLTERLAMVQPGETIALLFIDVDRFKTINDMYGHFVG
                     DQVLVELSKSLMRFKQPNNTLARLGGDEFIFAVHADYGYREAEKLAEQVVQACGETLN
                     IGNYAFSLTVSVGISIYPLDAENSDMLLKNADIAMYQAKKLGCNKYVSFSEELSGAIQ
                     RKNEIEMLLKTVDYDQEFKLFYQPQFSIPDKKLIGMEALLRWNSPQKGFIPPDEFIPL
                     AEETGCIMPIGDWVMQKAISQIDSWNHYYGMELQMGINVSPRQLDQSSFIHNLQSSMK
                     AHHVNPQWVDIEITEGVAMADKYRMPEIANQFKDAGVSISIDDFGTGYSSLSCLKLFP
                     FDRIKIAKPLIDAITSDNFDQNITKYTILMAKSVGIKTIAEGVESQKQFDLLTQLGCE
                     QIQGYFLGRPVPPSKFEEEFLKDRTEYRLAAEA"
BASE COUNT          698 a          611 c          686 g          747 t
ORIGIN      
        1 tatggtataa ttcgattata gtaagcggaa tttaaaaaat ttgtttcccg gatgttgcaa
       61 aagaacggct gactttccat tatttagcaa aatatttccg gacagcacca aaattaatcg
      121 gattttttac gaatagggtt atgtgtgccg aaaccaagaa ttttaaacga aactgaatgt
      181 gtgattgaaa tgaaaaacaa gacatggatt ccagcattgc ctgtgattgc ggtttccttc
      241 ctattatacc tgattgtgag cacactgcag aatgaatttt tcggggatat tttatccccg
      301 ttctgtgcgt tgctggcatc ggccattatt tttaatacat atttcaagtc cggccgtgcc
      361 cgcaagatca accggatttg ggccgcttta gggatggcat gcctttcctg ggctgccgcc
      421 gatattttat gggcgctttt tgattttgta tttcaccttg atccgcagga aagcgtcctg
      481 atttccattc tgtatatggg aacgaatttc tttctgtttg ccgcgatttt tatttattct
      541 gtttataaat ttcggaaatg gaacgcgatc cagctggttg tggatgccgt tatgatatcg
      601 gtgacgagca tctttctcct gtggatcctg ttttttgata aagaccctga aatgttcagc
      661 cggatcggca atgagggctg gatcacatcg gtaagtatta ttattgatgt tgccgcgttt
      721 atctgcattg tggtatggtg cttctccatt cacggcggga agcttccgcg ctatctgtac
      781 gtttctatcg gctcgatttt tctgtatatt gtttcagacc tgtattggtt ttatttggat
      841 attaataaca catatgctcc gaattccctg attgacgcgg cctacatggc ctcattgctc
      901 ggcattgctg tggcggcgga gatgttccgc gctgacagcg gagccggtga acgtgatttt
      961 tctaatgtgg gaagtaagaa caggggtctg tttctcttgt cgggcctgct cctgaccgtc
     1021 gtcttcaagg gttttgtcct gacggatctg ctggttttcc tttttattac acttgcctat
     1081 atcagcttta ccgtttatat tcagggctcc atcaaatccg accagcttct gagaaaggaa
     1141 tacgacctca acgccgagct ggaaagccgt attgcggacc gcacaaagga gctcgactat
     1201 ttgtccaacc gcgacctggt caccaatctt tacaaccgcc gcagctttat ccgccgcctg
     1261 acggaaaggc ttgcaatggt tcaaccaggc gagaccattg cgcttttgtt tatcgatgta
     1321 gaccgtttta aaacgatcaa cgatatgtat ggccattttg taggcgatca ggtgctggtg
     1381 gagctgtcaa aaagtcttat gcggtttaag cagccgaaca atacgctggc gcgcctcggc
     1441 ggcgacgagt ttattttcgc ggtacacgca gactacggtt accgcgaggc ggaaaagctt
     1501 gcggagcagg tggtccaagc ctgcggcgaa actcttaaca tcgggaatta cgcgttttcc
     1561 cttacggtca gcgtgggaat ttcaatttat ccgctggatg ccgaaaacag cgatatgctc
     1621 ctgaaaaacg cggatatcgc gatgtatcag gccaagaagc tcggctgcaa taaatacgtt
     1681 tcgttcagcg aagagctttc cggcgcaatc cagcgaaaga acgaaataga aatgcttctg
     1741 aaaacggtcg attacgatca ggaattcaag cttttttatc agccgcagtt cagcatccct
     1801 gacaaaaagc tgatcgggat ggaagcgctg cttcgctgga acagccccca gaaagggttc
     1861 ataccgccgg atgagtttat tcctctggcg gaagaaaccg gctgcattat gccgatcggc
     1921 gactgggtga tgcaaaaggc catcagccag atcgacagtt ggaaccatta ttacgggatg
     1981 gaactccaga tgggaatcaa tgtttcaccc agacagctgg accaaagcag ttttatccat
     2041 aacctgcagt cttccatgaa agcgcatcat gtgaacccgc aatgggtgga tatcgaaata
     2101 acggaaggcg tggcaatggc ggacaagtac cgcatgcccg aaattgcgaa ccagtttaag
     2161 gacgccgggg tttccatctc gatcgatgat tttgggacag gctattcctc gctgagctgt
     2221 ctcaaactgt ttccgtttga ccgcatcaag atcgccaagc cgctgattga cgccataacc
     2281 tcagacaatt ttgatcagaa tattaccaaa tatacgattt tgatggcaaa atccgtcggc
     2341 atcaaaacca ttgccgaagg cgtggaatcc caaaaacagt tcgacctgct gacccaactg
     2401 ggctgcgagc agattcaggg gtattttttg ggcaggccgg tgccgccttc caaatttgaa
     2461 gaagaatttt taaaggacag aaccgagtat aggctggccg cggaagcgta agtatagtcg
     2521 attaacttta aaaacagcta aggatttgcg aggatatttt tcgtgatgta aggcggacga
     2581 cgaagccagg ctggcgcctg gcgaggagga caacgcaaca gcacggaaaa tagaccgcaa
     2641 agactgggtg gctttggagt taattgacta tatgtatggc ttatggacta aaatagaatc
     2701 agttaaaaca aaccccgaaa taccgcctga gtggacggtg ct
//
LOCUS       DATALY010000017        24550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_2828,
            whole genome shotgun sequence.
ACCESSION   DATALY010000017 DATALY010000000
VERSION     DATALY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24550
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..834)
                     /locus_tag="VHR42_00485"
     CDS             complement(7..834)
                     /locus_tag="VHR42_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX3025697.1"
                     /translation="MPGLTVYEYGKSLLNSGEKACYSQIAQALKNVSPSVTVKTNITP
                     ETFEKIYQYYLYDHTEIFYLKNSTMKYDYLTVVGKKTYNSYTLTFQYRYNGKTVAAMR
                     KAMRQGALEMLASAESKSSALGKEKALHDTLVATCSYDSSAQTNKDLNSVSYTAYGIF
                     VNKKAVCDGYARALKILLSSAGIKSAYVTGTASNSAGEWEDHGWDMAYVSGRWYYVDA
                     TFDDPVYVDSQGREISGSGDRVSYSYFNFSSKSDHVLGKFNGSNPFDDNSQNYETMP"
     gene            1294..1476
                     /locus_tag="VHR42_00490"
     CDS             1294..1476
                     /locus_tag="VHR42_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025698.1"
                     /translation="MHCPDGIDGKEYVALVAAASILLSESLNAKDTFILAEFLQAVSY
                     QMFTLGAFKEFDRPHP"
     gene            complement(1479..2408)
                     /gene="ribF"
                     /locus_tag="VHR42_00495"
     CDS             complement(1479..2408)
                     /gene="ribF"
                     /locus_tag="VHR42_00495"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEX3025699.1"
                     /translation="MEVFLPNHEICLNGPTAVALGFFDGVHLGHAAVIQSARLTGLQT
                     VVITFLQHPGGKKTLRLMSNRQKEEAFQQLGVEIMAYLDFPQVKDLEPEEFVRDILDA
                     QFHAKKVFCGFNYHFGRGAVAGSEELKRICGKYSIETGCVEPVYSEIKGEDGPVPISS
                     TLIRGFVENGEMEQAAACLGRPYAFCYEVVSGRRLGRQLGTPTINQKFPEGFMIPRFG
                     VYASRAYAGGKQYPAVTNVGVKPTVGSDCVLAETYIIGYEGDLYGTEIKVELLSFLRG
                     EKKFDGLVQLKNQIGLDAEQSISICRTFERSNT"
     gene            complement(2383..3306)
                     /gene="truB"
                     /locus_tag="VHR42_00500"
     CDS             complement(2383..3306)
                     /gene="truB"
                     /locus_tag="VHR42_00500"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX3025700.1"
                     /translation="MNGIICIDKRAGLTSFAIVGRVRKLTGQRKIGHAGTLDPMATGV
                     LPVFMGRATKEIDILPCQDKTYVATVKLGVTTDTGDTTGHVLTETAVAVEPERFEQAA
                     LSLRGKIRQIPPMYSALKVNGRRLYDLAREGREVERQPREVTIYDICVLGRKAGDEYT
                     IQVSCSKGTYIRTLCEDIGSILGCGAAMSGLRRTVAAGFSIQECRTLEQLEDLSNEGK
                     LESVILSPEYPFRMLPEVLVTDKQTVRFLNGGALSLERVRCAPTNGLCRIKTKNRELL
                     GIAWVDIERSELKMKCLLSGGETDGGFPSES"
     gene            complement(3303..4289)
                     /locus_tag="VHR42_00505"
     CDS             complement(3303..4289)
                     /locus_tag="VHR42_00505"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX3025701.1"
                     /translation="MGFQQVKTPAYQQRMDEAVRFFTSADKILILSHLSPDGDTLGSA
                     FALCRALLKMGKKARVICSDPFPEKFKYMYDGLEMEEFSPAAIVSVDIASRNLFGRKL
                     EPFTDKVDFCIDHHPSNTGYAARTLLNPEAAATCEIILELVKLLGVEINNFIADCIYT
                     GLATDTGCFRYSNTTPQTLRCAAEMMERGAQAAKISKRMFETLSRPRLEMERQVLNSL
                     EYYYEGKVALVYVTREMCEKTGVPDSDLEGIPSIPVRIEGVLAGITIKEKSPEIYKIS
                     LRTNSGVNASEICSNFGGGGHAAAAGCQIEGSLAQVREKILEATGKALDGLK"
     gene            complement(4292..4633)
                     /gene="rbfA"
                     /locus_tag="VHR42_00510"
     CDS             complement(4292..4633)
                     /gene="rbfA"
                     /locus_tag="VHR42_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX3025702.1"
                     /translation="MPGHRMDRVTGDIKREVSEIIRGLKDPRVSGIVSIVKVEVTQDL
                     AYAKIYVSSVGSDLGQTVKGLNNAAGFVRKELSSRLTIRKTPEIKFIADDSIEKSARI
                     SKIIDEISKDD"
     gene            complement(4715..7165)
                     /gene="infB"
                     /locus_tag="VHR42_00515"
     CDS             complement(4715..7165)
                     /gene="infB"
                     /locus_tag="VHR42_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX3025703.1"
                     /translation="MMIKYRVHEVAKDLGIPSKNIIELLAKHYEEPRKHMTALSEEEL
                     NLIFEHYTKENSVENFDEFFAMRKQMAQQTMQSGEQVRPAAAQQQKNQPAAAGQSAQQ
                     PAAGNQAPGGNPANQVRRDNAQPGQQRTQPQQGNRPVAANQGKQQQPNAAASKNPASG
                     TRAVRYVDTRGAQVELEKYNEHYEEIAPNQTPKLAPQAKQKIRQKSADRRPPYSRKSS
                     EAEKMRRLALEKQKKPQLKISIPEEISVGELAARLKVTASQVIKKLMAMGVMASISQI
                     IDFDTAGVVAMELGAKFEKEIEITIEDQLIDDSEDKDTDLQPRSPVVVVMGHVDHGKT
                     SLLDAIRHSDVVAGEAGGITQHIGAYRVKINGSDITFLDTPGHAAFTAMRARGAQATD
                     IAVLVVAADDGIMPQTVEAINHAKAANVAIIVAINKIDKPGANPEKVKQELTEHGLVA
                     EEWGGDTICVPVSAVKHENIDKLLEMIQLVAEMKELKANPNRAGKGIVIEARLDKGRG
                     PVATLLVQNGTIHAGDVIIAGTTVGKIRAMTDEKGKRIEKAGPSVPVEVTGISEVPLA
                     GDIFHVVKDERMARELVEQRRQQEKEEQFSTYQKVTLDNLFAQMQEGEVKELNIIIKA
                     DVQGSVEAVRQSLEKLTNEEVRVKVIHGGVGAINESDVYLAESSNAIIIGFNVRPDTD
                     AQNRASQSGVEMRMYRVIYDCIEEIESAMKGMLTPKQREVQLGRVEVRHVYKISSVGT
                     IAGCYVTDGKVSRNAQIRVVRDGIVVTEDKIDSLKRFKDDAKEVMQGYECGIGLEKFN
                     DIKEGDIFEPFIIEEYHD"
     gene            complement(7140..7490)
                     /locus_tag="VHR42_00520"
     CDS             complement(7140..7490)
                     /locus_tag="VHR42_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEX3025704.1"
                     /translation="MEDKLFGMLGIARRAGKITFGFDAVVRSVTEGTAFLVLMSQDVA
                     ERTERQTIRACDENSVKAMKTKKSMLDIGYAIGRGSTAVLSVTDSSIAAKLEELCRDK
                     GEERTDYDDKVQGS"
     gene            complement(7480..7752)
                     /locus_tag="VHR42_00525"
     CDS             complement(7480..7752)
                     /locus_tag="VHR42_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX3025705.1"
                     /translation="MRQKKMPMRMCTGCGEMKPKRELVRVVKNNLGEISLDLTGKKPG
                     RGAYVCKNLECLKKARKTKRFERAFQCVIPDEIYARMEEELNADGG"
     gene            complement(7854..8999)
                     /gene="nusA"
                     /locus_tag="VHR42_00530"
     CDS             complement(7854..8999)
                     /gene="nusA"
                     /locus_tag="VHR42_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX3025706.1"
                     /translation="MNAEFFAALEALEKEKGIPEGYLAEKIANAINIAVKHNYKTGEN
                     TVVELDPAKKVFRVALRKTVVENVENPQMEILPEEARTIDPSASLGDEVDIELDTHDF
                     GRIAAQTAKHVIRQGIREVEREIITSEVMRRQHEMVTAMIQRVDKNTGTAYLELGRTE
                     AVMPTNEQIPGETLREGEHIKVYVAEVKNNEKGPKTIVSRTHPGVIRRLFELEVPEIF
                     DGTVEIMGIAREPGSRTKVAVDSKNPDVDAIGSCIGPKGNRVGNIVRELNGEKIDLIP
                     YSQDVSKYIAGALAPADVISVDSDRDEHRARVVVPDDQLSLAIGNKGQNVRLAAKLTG
                     WRIDIKARSAAETGDAAAEESDEKQTTMAAAFEAAEKLNEDGEPAEE"
     gene            complement(8989..9489)
                     /gene="rimP"
                     /locus_tag="VHR42_00535"
     CDS             complement(8989..9489)
                     /gene="rimP"
                     /locus_tag="VHR42_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX3025707.1"
                     /translation="MAVRKGGNVISAVEQIAIPVARELGLTLWDIEFVKEGASWFLRI
                     YIDKESGISVEDCEAFSHGIDKKLDEVDPIEQSYYLEVSSPGIERELKKDWHFEKMNG
                     KIITIHLIRPVDGRKEIRGVLKAFSKDSVTVLDGEEEISVQRQNAAHIRLFDEDFTGG
                     IEENEC"
     gene            complement(9615..10340)
                     /locus_tag="VHR42_00540"
     CDS             complement(9615..10340)
                     /locus_tag="VHR42_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HEX3025708.1"
                     /translation="MVEKYSMEFRVSSYDVDPARRLKLSSLLKVIQEAAGRHLDGDVL
                     TYEYMREKGIVFLLVKEAVKVYHMPKYNEPVTVETWFLKTKGAQFIRNLRFLGADGIL
                     AEAETYWVIVDPVSHRILRPSAYPFEMRSHEQKVGIHASKFDMPQNIEKVGVRPVRWS
                     DTDCNRHMNNAVYPDVICDFFPGGMNEREISTLQIHFLGEAVEGEEITVSAGTGKNGE
                     YYFRGETPGRACFEAFVISKKMK"
     gene            complement(10380..11654)
                     /gene="serS"
                     /locus_tag="VHR42_00545"
     CDS             complement(10380..11654)
                     /gene="serS"
                     /locus_tag="VHR42_00545"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3025709.1"
                     /translation="MLDIKRIRNEKEIVEKGLRKRGKEYDIDAVIKADDERRSLISQT
                     DLMKAEQNADSKKIPALKKEGKGDEADLLLKRMKELSDGIKNNDAKIRELEETLKNLI
                     LAIPNVPHESVPAGKNSDENIEIRRWSEPRNFGFEPKPHWEIGEQLGILDPETAAKVT
                     GARFHFYKGLGSRLERAIINFYLDFHSKNSGYTEIFPPYMVNRASMTATGQLPKFEED
                     AFKLANYDYFMIPTAEVPVTNMHRDEILDGGKLPIKYCAYSACFRAEAGSAGRDTRGL
                     VRQHQFNKVELVKFAKPETSYDELEKLTNDAEKVLQALGLAYRVVCLCGGDLGFSSAK
                     TYDIEVWMPSYNRYLEISSCSNFEDYQARRASIRYKETSRDKAKLVHTLNGSGVAVGR
                     TVAAILENCQNDDGSVTVPEVLRPYMGVDVIK"
     gene            complement(11743..12606)
                     /locus_tag="VHR42_00550"
     CDS             complement(11743..12606)
                     /locus_tag="VHR42_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3025710.1"
                     /translation="MKRGLGKGLDALFADNAVESARDANGSTMLRLSEIEPNRGQPRK
                     TFDEEALSQLADSIRQHGVLQPLMVRPNPGMNGYQLVAGERRWRAARMVGLTEVPVVI
                     KELDDLQVMEIGLIENLQREDLNPIEEAEGYRTLMETFHLTQDEVAQKVGKSRSAVAN
                     AMRLLNLPDRVSDMVRNGDLSQGHARTLLSLENPDQMLIVAEDIIKKGLSVRNVEKMM
                     KRGEKPQEQKQIKSTGGVVASEIERSLTEYLGRKVTVVSGKRRGTIEIEFYGEEDLKQ
                     LSQMIVESSNG"
     gene            complement(12603..13382)
                     /locus_tag="VHR42_00555"
     CDS             complement(12603..13382)
                     /locus_tag="VHR42_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3025711.1"
                     /translation="MSKVIAVANQKGGVGKTTTSVNLTAALGEAGKRVLLIDVDPQGN
                     ATSGLGVNKRNVKKSTYDLMIGNATAEEILIHTPFQNVDILPSNISLAAAEIELVESS
                     NREAVLKKALLPVRSNYDYIIIDCPPSLGLITLNALNAADTLLIPAQCEYYALEGLSQ
                     LMATVRMVKRLYNPSIDIEGVLLTMFDGRLNLTIQVVEEVKRFFPRKVYKTVIPRNVR
                     ISEAPSYGQPVLYYDKGSRGTEAYRDLAKEVMEMNKGVDSR"
     gene            complement(13759..13986)
                     /locus_tag="VHR42_00560"
     CDS             complement(13759..13986)
                     /locus_tag="VHR42_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HEX3025712.1"
                     /translation="MEKIKLTAEQKREAITEIRDYFATEREEEIGGLAAEMILDFITD
                     KIGKHYYNQGITDAQSYMTEKVEDLYGLLIL"
     gene            complement(14117..14932)
                     /locus_tag="VHR42_00565"
     CDS             complement(14117..14932)
                     /locus_tag="VHR42_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3025713.1"
                     /translation="MLGFTIKAKNNNRVISVPIGKIKPNPNQPRRQFEHEALMGLAES
                     IRHNGILQPLSVRPLEDGNFELVAGERRLRASLMAGFTQVPCIEVSLNDKQSAVMCLI
                     ENLQRQDLNFFEEADGIAKLIDTCGLTQEEIAHRLGKKQSTIANKLRLLRLTNGERKM
                     ILDHEMTERHARALLRLDDGVRESALRIIISKKLNVQETDKLIEQMLLPNQDKKTQRK
                     MPVIKDVRLFINTVSNAISVMKRAGIDAVAVSRDFDDYLEYTIRIPKAARKSA"
     gene            complement(15054..15773)
                     /gene="rsmG"
                     /locus_tag="VHR42_00570"
     CDS             complement(15054..15773)
                     /gene="rsmG"
                     /locus_tag="VHR42_00570"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX3025714.1"
                     /translation="MNEELKGTLIRSAEALKVDLDNRAIERFSIFMEELQSWNQRMNL
                     TAITEPGEIITKHFVDSLTVRPYLEGSKVDLIDIGCGAGFPGIPLLISDNRIFLTCLD
                     SLMKRLNFIEQLMPKLEITQYQCIHGRAEELGRMKEHREQYDIAVSRAVAQLSKLLEY
                     CLPLVKKGGKFLAMKGPDYREEMSDIQKAVKLLGGEIESADEITLPLTDMKRSIIAIR
                     KIKPTPQRYPRQQAKINKMPL"
     gene            complement(15813..17696)
                     /gene="mnmG"
                     /locus_tag="VHR42_00575"
     CDS             complement(15813..17696)
                     /gene="mnmG"
                     /locus_tag="VHR42_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX3025715.1"
                     /translation="MEYQAGNYDVAVIGAGHAGIEAALAAARLGCKTVIFTINLDAVG
                     NMPCNPSIGGSAKGHLVREIDALGGQMGKAADQTFLQSRMLNRGKGPAVHSLRVQSDR
                     REYQKVMKHTLELQKGLDLKQAEVIDVRVKDGKVESVVTKLGAVYKVKAVILATGTYL
                     KGKIHIGEISYDGGPDGMFGADALSDCLKRLGIEMMRFKTGTPARVNRSSINFDKLEE
                     QQGDEKVVPFSFETEGEFSNQVVCHIAYTNNQTHEVIRENLSRSPLYSGRIEGVGPRY
                     CPSIEDKVVRFADKERHQIFVEPLGIDTEEMYLGGMSSSLPEEVQQKFLQTISGFENI
                     KMMRTAYAIEYDCVNPLELKHTLEIKKIIGLFGAGQFNGTSGYEEAAAQGLVAGINAA
                     LEVGGREPLVLDRSESYIGTLIDDLVIKGTNEPYRMMTSRSEYRLVLRQDNADERLTP
                     TGYKIGLIAEQRYRKFQEKMNLIRQEKERLEKTNVPPSHQINEFLCSRHTAPLSSGIR
                     LSELLKRPQIDYDSLTDYDLERKALPEPVTEQVEIQLKYEGYIKRQMTQIKEMQRLEI
                     KKIPKSLDFDNIRGLRLEARDKLKRIRPENIGQASRISGVNPADIAVLLIYLEANLSG
                     VGQ"
     gene            complement(17721..19082)
                     /gene="mnmE"
                     /locus_tag="VHR42_00580"
     CDS             complement(17721..19082)
                     /gene="mnmE"
                     /locus_tag="VHR42_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX3025716.1"
                     /translation="METIAAIATPVAAGGIGIVRISGENAFEIAQKIFRSKNSKEIVE
                     MKPYTAAYGKIIDGEKEIDEGIALVFHAPKSYTGENVVELSCHGGIMVMRMVLKAAIL
                     AGARLAEPGEFTKRAFLNGKLDLTKAESVMDLINAQSKMAVNAARMQHEGALFSSIML
                     IKSHLIEISSDISAWMDFPDDEMEGLQEDNLKSSIKQIADKMKRLIATYETGKMIREG
                     IDTVITGSPNVGKSTLMNLLSGYEKSIVTEIPGTTRDIVEGRVVLGDLILNLSDTAGL
                     RQTDDPIEKIGVERAKSKLENADLILAVFDSSRELDEEDMKLIDFISNKLTVAIINKA
                     DLPKKIDDKYILNKIQHTVYISAKTHEGIKELEKVAAEIIGTNQLDLGAGILANERQY
                     QSVNDAEKGLEEALFALESGMTLDAISIGIDEAIGCLSALTGEKASDEIIQRVFEKFC
                     VGK"
     gene            complement(19158..19817)
                     /gene="jag"
                     /locus_tag="VHR42_00585"
     CDS             complement(19158..19817)
                     /gene="jag"
                     /locus_tag="VHR42_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX3025717.1"
                     /translation="MKYEAIGTGRTVEEAIRIACESLGVEREQVEFEILELPSKRLLG
                     LLGASDAKVKVVYNEGVEDKARNYLKQILEKMELPGVIIESTETEKGINFDLAGEGLG
                     IIIGHRGETLDSLQYLVSLVANKGEEQYKRITIDTGNYREKREKTLMALAKRIALNSI
                     RTKRTTTLEPMNPYERRIIHTAVQEIRGAASWSVGEEPNRRVVIGAENASRAPQKMFR
                     S"
     gene            complement(19838..20983)
                     /locus_tag="VHR42_00590"
     CDS             complement(19838..20983)
                     /locus_tag="VHR42_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX3025718.1"
                     /translation="MNLFADIGNFFNMVIYRPLGFLLGACYQVGRNYGIALILFTLVT
                     RVLMIPLAIKQQKSSAEMIRMQPKMAEIQKKIKDKTKQAEELQKLYQEEGYSPFAGCL
                     PLLIQLPIIYGLFGVIYKPLTYILGLTAVQVNKVVATLTPQIQTMLHHAINSGDQSKI
                     EIYAAQAMNGNMDKLSFLPHKIMALDFNFLGVDLSKTPSLSLNILILIPILCYVTNFL
                     YMWVNMKMMATPTTQNNPMNNKMMMVLMPIMSTVFSLQLPAGIGFYWIITNLFMIVQV
                     YVINIFYNPKKLAEQAEIAAKKRKDARAAARKAAIAANKSDDKVTEGSDKPSGISDTA
                     EAGPDEEKKKSAKALPQEMKSKKQIKEENRKRLAASRKMEKRGGQDK"
     gene            complement(21058..21303)
                     /gene="yidD"
                     /locus_tag="VHR42_00595"
     CDS             complement(21058..21303)
                     /gene="yidD"
                     /locus_tag="VHR42_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX3025719.1"
                     /translation="MKGVFIRLIRFYQRYLSPLKPPSCRFYPTCSNYAITAISRFGVL
                     RGGLLSMRRILKCHPFHPGGVDPVPLIYPSEKICKRN"
     gene            complement(21300..21638)
                     /gene="rnpA"
                     /locus_tag="VHR42_00600"
     CDS             complement(21300..21638)
                     /gene="rnpA"
                     /locus_tag="VHR42_00600"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3025720.1"
                     /translation="MQQTVSLKENREFRRLYKKGRSYVSPVVVVYMLKNKKQYNRIGI
                     TTGKKLGKAVIRNRVKRIIREAYSGVETQTKTGWDFVFVARNRAVNLKSWQMMKTLKG
                     LFIQAGVWKK"
     gene            complement(21766..21900)
                     /gene="rpmH"
                     /locus_tag="VHR42_00605"
     CDS             complement(21766..21900)
                     /gene="rpmH"
                     /locus_tag="VHR42_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX3025721.1"
                     /translation="MIRTYQPKKIHRNKEHGFRKRMATKNGRKVLARRRAKGRKQLTY
                     "
     gene            22360..23703
                     /gene="dnaA"
                     /locus_tag="VHR42_00610"
     CDS             22360..23703
                     /gene="dnaA"
                     /locus_tag="VHR42_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX3025722.1"
                     /translation="MQSFKEVWSQVCQYCKGKISDVAFKTWIYCMEPVELKSDEAVFF
                     VRSDFQRNIIFDRYKELLIDGFESVLGFKVDINIVTEEDKPNGYRSQYDFVITQNPED
                     IEKSYASADYEYTFDTFIVGSSNKFAHAASLAVASNPAMAYNPLFIYGGSGLGKTHLL
                     HAIKYEIEKNDPGSNIIYIKGDDFTNELVESIRQNSQIQFRNKYRQSTVLLVDDIQFI
                     AGKEGTQEEFFHTFNTLYEAKKQIVLTSDRPPKEIQTLEDRLRTRFEWGLLADVQPPD
                     FETRLAIIKRKAELLGISIPDEVSEYIANKLKNNIRQLEGAVKKMKAYNLLAGDPPSI
                     AIAQNAIRDILNDNQPVPVTVDRIIGEVSRTFGVTGDELRGKMRSGNISSARQVAMYI
                     VRDITQMSTSSIGDEFGGRDHSTVVYAIQKVEDDMSSNQRFRATVQDIIKNIRDH"
     gene            24021..>24550
                     /gene="dnaN"
                     /locus_tag="VHR42_00615"
     CDS             24021..>24550
                     /gene="dnaN"
                     /locus_tag="VHR42_00615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX3025723.1"
                     /translation="MELSCNKKDLAEAVSTVQRAVSTKSSLPALEGILIKASEHVLTL
                     SAYDLELGISKSISASVSQPGEIVLNARIFADMVKKLPAEQVELKTDEKLLTTVNSGN
                     SEFTFLGIPSSEFPEIPSLTDGTMFTLPQSLLSSMIRQTLFAVSSDDTKPIHTGSLFE
                     IDNGVFNIVSVDGYRLA"
BASE COUNT         5900 a         6410 c         4795 g         7445 t
ORIGIN      
        1 ggtcctttat ggcattgtct cataattttg cgaattgtcg tcaaacggat ttgacccatt
       61 aaattttccg aggacatgat cggattttga actgaaatta aaataactgt aactgaccct
      121 gtcgccggac ccggatattt cccgcccctg gctgtcaaca tagacgggat cgtcaaaagt
      181 tgcgtcaaca taataccacc gcccggaaac atacgccata tcccatccgt gatcttccca
      241 ttcgcccgcg ctgttcgaag ccgtacccgt tacatacgcc gatttaatac cggcagatga
      301 cagaagaatc ttcaaagccc tggcataacc gtcgcaaact gcttttttat taacaaatat
      361 cccatatgca gtatagctga cgctatttaa atccttattt gtctgggcgc tgctgtcata
      421 gctgcaagtt gccaccaagg tatcatggag cgccttttcc ttccctaagg cgcttgattt
      481 gctttcggcc gaggccagca tttcaagcgc cccctgccgc atcgccttcc tcattgcggc
      541 gaccgttttg ccgttatagc gatactgaaa ggtcagcgtg taggaattat atgtcttttt
      601 ccccactact gtgagatagt catatttcat tgtcgagttt ttcagataaa agatttccgt
      661 atggtcataa agataatatt gatagatttt ttcaaatgtt tcaggggtta tgttggtttt
      721 gaccgttacg ctgggcgaca catttttcag agcctgagca atttggctgt aacaagcctt
      781 ttcaccggag ttcagcagtg actttccata ctcataaacc gtaaggcccg gcattccggt
      841 atccccccag aagctgcccg aatcagctgc tatatcctgt ccggctgatt ctgactgtga
      901 accgttagac ccattcgaaa acagcaaaga aaactcagcc agaaaattct tcccggctaa
      961 cgcggaaaaa gctgactgga tttggctttt tgcactgttt aagcaaccgg acgcatccgt
     1021 gaaaatgttc ttcccattcg ccaatgatag aaaaaggaca gcagtcaata caatgactac
     1081 tgtcgagaaa aaagcttttg caaaagtttt cataaaatcc tccagacact tcaggacaaa
     1141 agacagcgct tttttcccgg ccgataaatt ttatgtcgca tcacaaattg gtcatagtcc
     1201 gcttttgcct ggtcaatatc agtgctccat taataatcag cacaatgatt tccatcatat
     1261 attaaagata tacggatgtg gggagggtca ttcatgcatt gcccagacgg cattgacggt
     1321 aaggagtacg tcgctctggt tgcggccgca tctattctgc tctccgaaag ccttaacgcg
     1381 aaagacacct ttattctggc tgagtttctt caggcagtca gctatcagat gtttaccctc
     1441 ggcgcgttta aagagttcga ccgtcctcac ccataatcct atgtattgct ccgctcaaag
     1501 gttcggcaga ttgagatcga ctgctcagcg tcaaggccga tctggttttt cagctggaca
     1561 agtccgtcaa actttttttc accgcggaga aacgaaagaa gctctacttt aatttctgtg
     1621 ccgtaaagat ccccttcgta cccgataata taagtttctg ccaaaacaca gtcgcttccg
     1681 acggttggct tgaccccaac gttcgttaca gccggatact gtttgccccc cgcataagcc
     1741 ctcgatgcgt aaacaccaaa gcgcggtatc ataaaccctt ccgggaattt ctgattgatt
     1801 gtgggcgttc ccagctgcct tcccaatctg cggccgctca ccacttcgta gcaaaacgca
     1861 tacggccttc caagacaggc ggctgcctgt tccatctctc cgttttccac aaagccgcga
     1921 atcagcgttg aactgattgg tacgggcccg tcctctccct tgatctctga gtagacaggt
     1981 tccacacagc ctgtttctat cgaatatttc ccgcaaattc tcttaagttc ttccgaacct
     2041 gctaccgctc ctctgccaaa atggtaattg aagccacaaa aaaccttttt tgcatgaaat
     2101 tgggcatcta ggatgtcccg tacaaattcc tccggctcca gatcttttac ctgggggaaa
     2161 tcaagataag ccatgatctc cacgccaagt tgttggaacg cttcttcctt ctgccggttg
     2221 ctcatcagcc taagcgtctt tttaccgccc ggatgctgca gaaaggtaat caccactgtt
     2281 tgcaagccgg ttagtctggc actttgaatg acagcggcat ggcccagatg tactccgtca
     2341 aagaagccga gagcgacagc tgtcggcccg tttaagcata tttcatgatt cggaaggaaa
     2401 acctccatcg gtttcacctc cggataaaag gcatttcatt tttaattccg acctttctat
     2461 atctacccag gctattccca gaagttcacg gttttttgtc ttgatccggc acaaaccgtt
     2521 ggttggggca cagcgcactc tttccagtga caaagctccg ccattgagga atctgaccgt
     2581 ctgcttgtct gtcaccaaaa cttcgggcag catccggaac ggatactcgg ggctgagtat
     2641 gaccgattcc agtttaccct cgttcgagag atcctccagc tgctccaacg ttctgcattc
     2701 ctgaatggaa aaaccggcgg caacagtcct ccttaagcca gacatcgcgg cgccgcaacc
     2761 cagaatcgag ccaatatctt cacacagcgt ccgaatataa gtgcctttgg agcaggatac
     2821 ctgaatggta tactcgtccc cggcctttct tcccagtacg caaatatcat atattgttac
     2881 ctctctgggc tgccgctcaa cttctctgcc ctcccgggcc agatcataaa gccgcctgcc
     2941 attaactttc agagcggaat acatcggagg gatctgccta atttttcctc ggagagacaa
     3001 ggctgcttgc tcaaaccgtt ccggctcaac ggcaactgcg gtttcggtca aaacatgacc
     3061 cgtggtatcg cccgtatcgg tcgtgactcc aagtttaaca gtcgccacat aggtcttatc
     3121 ctgacaggga agaatgtcga tttctttggt agcacggccc ataaagaccg gcagaacgcc
     3181 cgtagccata ggatcaagcg ttcccgcatg tccaatttta cgctgaccgg taagctttct
     3241 gacacggccg acgatagcaa aagaagtcag ccccgccctt ttatcgatgc aaataatccc
     3301 gttcatttca gaccatccag tgcctttcct gtggcttcga ggattttctc tctaacctgc
     3361 gcgagactgc cttcaatttg gcagccggcg gcggccgcat gcccgccgcc gccaaaattc
     3421 gaacatatct ccgacgcgtt gactccggaa ttggtgcgaa gcgaaatctt atagatttcc
     3481 gggctttttt ccttgatggt tattcccgca agcacccctt caattcgaac cggtatggat
     3541 ggtattccct cgaggtccga atccggaacg cctgtttttt cacacatttc ccgcgtaaca
     3601 taaaccagcg cgacttttcc ttcataataa tattcgagtg aattgagcac ctgtctttcc
     3661 atctccagcc tcggtctgga aagcgtctca aacatacgct tgctgatttt cgccgcctgt
     3721 gcgcctctct ccatcatctc cgcagcacac cgcagcgtct ggggagttgt attggaatac
     3781 cggaagcaac cggtgtccgt tgcaaggccg gtatagatac aatcagcaat aaaattattg
     3841 atttctactc cgagcaattt tactaattct aaaataatct cgcaggtcgc agccgcttcc
     3901 ggattgagga gcgttctcgc ggcgtatccg gtattagaag gatgatgatc aatacaaaaa
     3961 tcaactttat ccgtgaatgg ctccagtttt cgcccaaaga gatttctaga tgcaatatcc
     4021 accgacacga ttgcggcagg agagaactcc tccatttcaa gcccatcata catatattta
     4081 aatttttcgg gaaaaggatc cgaacaaatc acgcgggctt ttttacccat tttgagcaga
     4141 gctctgcaaa gtgcaaacgc cgatccgaga gtatcgccgt cgggcgaaag gtgggataag
     4201 atcagaattt tgtcggcaga tgtgaaaaat cttacagcct catccattct ctgctgatag
     4261 gcaggagttt taacctgttg aaagcccact tttaatcatc ctttgaaatt tcatcgatta
     4321 ttttagagat cctcgcgctt ttttcgatgg aatcatccgc gataaattta atctccggcg
     4381 ttttgcgtat ggtaagacgg ctggaaagct ccttgcgcac aaagcccgcg gcattattca
     4441 ggcctttgac agtctggccg agatcgctgc cgaccgagct gacataaatt ttggcataag
     4501 cgagatcctg ggtcacttca accttgacta tactcacgat acccgaaact ctggggtctt
     4561 taagccctct gataatttcg gaaacctccc gtttaatgtc tccggtgact ctatccattc
     4621 tatgccccgg cattcaaaca ccttctttac acgaatcatt ttaaagtcct catgcaaata
     4681 taacaacttt gcgtctgttt ataactgtat tatactaatc gtgatactct tcgatgataa
     4741 aaggctcgaa tatatcgcct tccttaatat cgttgaattt ctcgagacct attccgcatt
     4801 cgtaaccctg catgacctct ttcgcgtcat ccttaaaacg tttcagcgaa tcaattttgt
     4861 cttccgttac gactatgcca tcccggacaa cacgaatctg ggcgttacgc gaaaccttac
     4921 cgtcggtcac ataacatccg gcaattgttc cgacactgga aatcttgtat acatgacgta
     4981 cttccacgcg accaagctgc acttctctct gcttgggtgt gagcatgccc ttcatggcag
     5041 attcgatttc ttcaatgcag tcatagataa cgcggtacat tctcatttca acgccgctct
     5101 gagaagctct gttctgtgca tcggtatcgg gtcttacatt aaatccgata ataattgcat
     5161 tggatgactc cgcaagatat acgtcggatt cattgatcgc cccaacgcct ccatggatca
     5221 ccttaacccg aacttcctcg ttcgtcagtt tttcaagcga ctggcggaca gcttccaccg
     5281 agccctgaac atccgccttg atgatgatat tcagttcctt gacttctccc tcctgcattt
     5341 gggcaaaaag attgtcaagg gttactttct gataggtact aaactgctcc tctttctctt
     5401 gctgacggcg ctgctctaca agctcgcgtg ccatacgctc gtctttaaca acatggaaaa
     5461 tgtcgccggc cagcggaact tccgatatac cggttacttc aaccggaacc gaaggtccgg
     5521 ctttttcaat gcgttttccc ttctcgtcgg tcattgcgcg aatttttcct acggtcgttc
     5581 cggcaatgat cacatcacct gcgtgaatgg ttccgttttg aaccagcagg gtcgccacag
     5641 ggcctctccc tttatcgagc cttgcttcta tcacaattcc cttaccggcg cggttcgggt
     5701 tggctttcag ctctttcatt tcggcaacca actgaatcat ttccagcagc ttgtcgatat
     5761 tttcatgttt cacagcggaa accggcacac agatggtgtc gccgccccat tcttcggcga
     5821 ccagaccatg ctcggtcagc tcctgcttaa ccttttcagg attggcgccg ggtttatcaa
     5881 ttttattaat ggcaacaata attgccacat tagcggcctt tgcgtggtta atcgcttcaa
     5941 ccgtctgggg cattatgccg tcgtcggcgg caaccaccag aacggcaata tcagtagcct
     6001 gcgcacctct tgccctcatc gctgtaaaag cggcatgtcc cggtgtatca aggaacgtaa
     6061 tatcgctgcc gttaattttg acgcgatagg cgccgatatg ctgcgttatg cccccagcct
     6121 cacccgcaac aacatcactg tgacgaattg catccagaag cgaagtcttt ccgtggtcga
     6181 cgtggcccat gaccactacc acagggcttc ttggctgaag atccgtgtct ttatcttcac
     6241 tgtcgtcaat cagctgatct tcaatcgtga tttcgatctc tttttcaaat ttggccccaa
     6301 gctccatagc caccacaccg gccgtatcaa aatcaatgat ctgtgaaatg gaagccatca
     6361 cgcccattgc catgagtttt ttgatcactt gagaagcggt tactttcagt ctcgctgcaa
     6421 gttcgcccac agagatttcc tcaggaattg aaattttgag ctgcggtttt ttctgcttct
     6481 caagtgcaag cctgcgcatc ttctccgctt cactgctttt acgcgaataa ggcggtctgc
     6541 ggtctgcgga tttctgccgt attttctgct tggcctgagg agccagtttc ggcgtctgat
     6601 tgggggcaat ctcctcataa tgctcattgt atttttcgag ctccacctgt gcgcctctgg
     6661 tgtcgacata tctcaccgca cgagtaccgg atgccgggtt tttcgaagcg gcagcgttcg
     6721 gctgctgttg tttaccctga tttgcggcaa caggcctgtt accctgttga ggctgtgttc
     6781 tttgctgccc cggctgcgcg ttatctctcc gaacctgatt cgccgggtta ccaccgggag
     6841 cctgatttcc ggcagcaggc tgctgtgcgc tttgacctgc cgcagcgggc tggttttttt
     6901 gctgctgcgc agccgccggg cggacctgct ccccgctttg catcgtctgc tgggccatct
     6961 gcttgcgcat agcaaaaaat tcatcgaaat tctcaactga attttctttg gtgtaatgct
     7021 caaaaatcag attaagctct tcctcgctta aagccgtcat atgttttctg ggttcttcat
     7081 aatgctttgc taaaagttcg ataatattct tgctgggtat tcccaggtct ttggcaacct
     7141 catgaaccct gtactttatc atcataatct gttctttcct cccctttatc ccggcaaagc
     7201 tcttcaagtt tcgcagcaat cgaactgtct gttacagaca gaaccgcggt tgagcctctg
     7261 ccgatcgcat agccgatatc cagcattgat ttctttgttt tcatcgcctt cacgctgttt
     7321 tcatcgcagg cccgaatggt ctggcgttca gtccgctccg caacatcctg actcataaga
     7381 accagaaagg ccgtgccttc tgtaacggac ctgacgacgg cgtcgaaacc aaaggtaatc
     7441 tttcccgctc tgcgggcgat tcctagcatt ccgaacaatt tatcctccat cggcgttcag
     7501 ctcctcttcc attcgtgcat aaatttcgtc gggtatcacg cattgaaagg ctctttcaaa
     7561 cctttttgtt tttcttgctt ttttcaagca ttctaaattt ttacatacat aggctccccg
     7621 tcccggtttc ttaccggtca ggtcaaggga tatttcaccc agattatttt ttacaacccg
     7681 gacaagttct ctcttgggtt tcatttcccc gcagcccgtg cacatgcgca tgggcatttt
     7741 tttctgccgc attttatcac cccagacgaa cgctgcaatc agctgtttcc gtttccttta
     7801 tatcataagt gtttgctaaa attttatcgc catgccaatt tccaaccgag attttattct
     7861 tcggcaggct caccatcttc attcagcttt tccgccgctt caaacgcggc tgccatcgta
     7921 gtttgcttct catcggattc ttccgcggca gcatcgccgg tctcggctgc gcttctggct
     7981 ttgatgtcaa ttctccaacc ggtcagtttc gcagcaagcc ggacattctg gcctttgttt
     8041 ccgattgcaa gagagagctg atcatcgggc acaaccactc gggcgcgatg ttcgtcgcgg
     8101 tctgaatcta cgctgattac gtcggcaggc gcaagcgctc cggcgatata ttttgaaaca
     8161 tcctgactgt aaggaatcag gtcgattttt tcgccgttta attctcttac aatattaccc
     8221 acacggtttc ccttaggccc aatgcaggag ccaatggcat ccacatcggg atttttggaa
     8281 tccactgcga cctttgttct cgaaccgggc tcgcgggcaa tccccattat ttcaacggtt
     8341 ccgtcgaata tttccggaac ctccagttca aacaatctgc ggattacgcc gggatgtgtt
     8401 cttgaaacaa tggttttggg ccctttttca ttatttttaa cttccgcgac atagaccttg
     8461 atgtgttcgc cctcacgaag cgtttccccc ggaatctgct cgttggtcgg catcaccgct
     8521 tcggtgcggc cgagctccag ataggcggtc ccggtgttct tgtcgactcg ctggatcatg
     8581 gccgtcacca tttcatgctg tctgcgcatt acttcgctgg tgataatttc tctttccact
     8641 tcgcggattc cctgacgaat aacatgtttg gccgtctgag ccgcaattct gccgaaatca
     8701 tgcgtatcga gctcaatatc cacttcgtct cccaaagaag cagaagggtc gattgttctg
     8761 gcttcctcag ggagaatctc catctgggga ttttccacat tttccacaac cgttttgcgt
     8821 aaagcaaccc gaaacacttt tttcgccgga tcgagttcaa ctaccgtatt ttctcctgtt
     8881 ttataattat gcttgactgc aatattaatt gcatttgcta ttttttccgc gagatatcct
     8941 tccgggatac ctttctcctt ttccagtgct tcaagagctg caaaaaactc agcattcatt
     9001 ttcctcaatt cctccagtaa aatcttcatc aaatagtctg atatgagcgg cattctgccg
     9061 ctgaaccgaa atttcctctt caccatcgag gacggttacg ctgtctttgc tgaaagcttt
     9121 cagaacgccc ctgatttcct ttcttccgtc taccggcctg atcaggtgaa tcgtaatgat
     9181 cttgccgttc attttttcaa aatgccagtc tttttttagt tccctctcaa ttccgggcga
     9241 agatacttcc agatagtaac tttgctcaat cggatccacc tcgtccagtt ttttatcgat
     9301 gccgtggctg aatgcctcac agtcttcaac tgaaatgcca ctttctttat cgatatatat
     9361 cctcaaaaac caggaggccc cttctttgac aaactcgatg tcccagagcg tcaagcctag
     9421 ttcccgggct acgggaatcg caatttgttc gaccgcggaa ataacatttc cgcctttacg
     9481 gacagccatg gatatccctc caaaataaac gaaagagtgg gtttccccac tcttttctcc
     9541 ttcgtaacaa acctattata gatgatttcc tgataaaaag caataacttt ttggatttct
     9601 tccaaaattt ttccctattt cattttttta ctaatcacaa acgcttcaaa acaggctctg
     9661 ccgggggtct caccgcgaaa atagtattct ccgtttttcc ccgttccggc tgaaaccgta
     9721 atttcctcgc cttccaccgc ttcgccgagg aaatgaatct gcagcgtgga tatctcccgt
     9781 tcgttcattc ctcccggaaa aaaatcgcag atgacgtccg gatagaccgc gttgttcata
     9841 tgccggttgc aatcggtatc cgaccatctg acaggccgta ctcccacttt ttctatattc
     9901 tgcggcatat caaatttcga tgcgtgaata ccaacctttt gttcatggga tctcatctca
     9961 aaaggatagg ccgacggccg aagaatcctg tggctgaccg ggtcgacgat gacccagtag
    10021 gtttccgcct cggccaggat accgtccgct cccagaaatc gtaaatttct gataaactgg
    10081 gctccttttg tttttaaaaa ccatgtttcc accgtcaccg gttcattgta tttcggcata
    10141 tgatagactt ttaccgcctc tttcaccagc agaaacacaa tgcctttttc ccgcatatat
    10201 tcataagtca gcacatctcc gtcaagatgc cggccggcgg cttcctgtat cactttcaga
    10261 agggacgaca gtttcagccg tcttgccggg tcaacatcat aggatgacac acgaaattcc
    10321 attgaatatt tttcaaccat aactgactcc ccagcaagca gcccggatag aaccggactt
    10381 tatttaatca catccacacc catatatggt ctgagaactt ccggaaccgt gactgagccg
    10441 tcatcattct gacagttttc caaaattgcg gcaaccgttc tgcccaccgc aacgccggaa
    10501 ccgttgagcg tatgaacaag cttggccttg tcgcgggaag tctctttata gcgaatcgac
    10561 gccctgcggg cctgatagtc ctcaaaattc gagcaggagg aaatttctaa atagcggtta
    10621 taggatggca tccagacttc gatgtcatag gtcttagcgc tggagaatcc cagatctccg
    10681 ccgcaaaggc agacaacgcg gtaggcaagc ccaagcgcct gaaggacttt ttccgcatcg
    10741 ttcgtcagtt tttccaattc atcataggat gtttccggtt ttgcgaattt aacaagctca
    10801 accttgttaa attgatgctg gcgaaccaac ccccgggtat ccctgccggc cgatcccgcc
    10861 tctgccctga aacatgcgga ataagcgcag tatttaatag gaagttttcc tccgtccaaa
    10921 atttcatctc tgtgcatatt ggtaaccgga acctcagcgg tcgggatcat aaaataatca
    10981 tagtttgcca gtttaaaggc atcctcttca aatttcggca gctggcccgt cgcggtcatt
    11041 gaagcacgat tgaccatgta gggagggaaa atttctgtat atccgctgtt tttcgaatgg
    11101 aaatcaagat aaaaattgat gattgccctc tcaagtcttg aaccaaggcc cttataaaaa
    11161 tgaaatcttg cgccggtgac ttttgccgca gtctcgggat cgagaattcc aagctgctca
    11221 ccgatttccc agtgaggttt tggctcgaat ccgaaatttc tcggttcgct ccagcgcctg
    11281 atttctatat tttcgtcact gtttttcccg gcgggcacgc tttcatgcgg aacattcgga
    11341 atcgccaaaa tcagattctt aagggtttcc tcaagttccc ggatttttgc atcgttattt
    11401 tttataccgt ccgagagctc tttcattctt ttgagcagga gatcggcttc gtcgcctttc
    11461 ccttcttttt tcaaagcggg aattttcttg ctgtctgcat tttgctcggc tttcatcaga
    11521 tcggtctggg aaatcagact tcttcgctcg tcatccgctt tgattacagc gtcgatatcg
    11581 tattcttttc ccctcttacg cagccctttt tccacaattt ccttttcgtt tctgattctc
    11641 ttaatatcca gcattccaat acacttcctt atcgttctca accagttaca ttttatcggt
    11701 ctttactcag ttctctaatt gaaacaggtt cttttatttt cctcagccgt ttgaactttc
    11761 cacaatcatt tgggaaagct gcttcaggtc ttcttcacca tagaactcga tttctatcgt
    11821 tcctctgcgt ttgccggaaa ccaccgtcac ttttcttccc aggtattccg tcagactgcg
    11881 ctcaatctca gaagccacaa caccgccggt tgattttatt tgcttctgtt cctgaggctt
    11941 ttccccacgt ttcatcattt tttcaacatt acgaacggaa agcccctttt taatgatatc
    12001 ttctgcaaca ataagcattt gatccggatt ttccaacgac agaagcgtcc tggcgtggcc
    12061 ctgtgaaaga tctccgttcc gaaccatatc gctcactcta tccggaagat tcagcagcct
    12121 cattgcgttt gcgaccgcgg aacgtgattt tccgaccttt tgagccacct catcttgcgt
    12181 taaatggaat gtttccatca atgtccgata gccttcggct tcttcaatcg ggttaagatc
    12241 ttccctttga aggttttcaa ttaaaccgat ttccatgacc tgaagatcgt ccagctcttt
    12301 tatcacaacc ggtacttccg tcagcccgac catccgcgcc gcccgccatc ttctctcacc
    12361 cgcaaccagc tggtaaccgt tcatgccggg atttggcctt accattagcg gctgcagcac
    12421 cccatgctgc ctgattgaat cggcaagctg cgaaagcgct tcctcatcaa atgttttgcg
    12481 gggctggccg cggtttggct caatttctga aagccttaac atcgttgaac cattcgcgtc
    12541 acgagccgat tccacggcgt tatccgcaaa taaggcgtca agcccttttc ccaagcctct
    12601 tttcatcgag aatccacccc cttattcatt tccattactt ctttggccag atcccgatag
    12661 gcttcggttc cccttgagcc tttgtcataa tataataccg gctgcccata actcggagcc
    12721 tcagatattc ttacatttct tggaattacc gtcttgtaca cctttcgggg aaagaatcgt
    12781 tttacctctt cgactacctg aatcgtaaga ttcagcctgc cgtcaaacat cgtcagaaga
    12841 acgccttcga tatcaattga ggggttgtaa agccgcttta ccatacgaac cgtagccatc
    12901 agctgtgaaa gcccttccag cgcataatat tcacattgtg cgggaatcag cagcgtatca
    12961 gcagcattca gtgcatttaa agtgatcagg ccaagcgatg gcggacagtc aataatgata
    13021 tagtcataat tggatcttac cggaagcaaa gcctttttta acacggcttc tctgtttgag
    13081 gattccacca gctctatttc cgcggccgcg agcgatatat tggacggaag aatgtcaaca
    13141 ttttgaaacg gtgtgtgaat caggatttcc tcggccgttg cgtttccgat cattaaatca
    13201 taagttgact ttttaacgtt tcttttatta actccaagac cgctggtggc gttgccctgt
    13261 gggtccacat caataagcag cacccttttt cctgcttcac ccaatgctgc cgttaaattc
    13321 accgaggttg tggttttccc taccccaccc ttctgattgg ccaccgctat cactttgctc
    13381 attccatcgc tccattcaat ctgaaacgtc attttgacgt gattattaca atgtatcttt
    13441 gtatatgagc ctacgctgac ggcccattcg tatcgcgcta tcgtttattt atccacaatc
    13501 gacttcgttg cctttttatg tccaactcac aatctaatcc attgctattc ctgggaaaat
    13561 cgcacatttt ccgaccgggc atttaaaaaa tcagtttgaa gccatatctt tctaaccaga
    13621 gaaagtagta attttagcct gtcatgccta attctggcct cgtaaagccg acttttacat
    13681 tatatcacac caaacaggcg atggaaagtt taaatatgca caagaacatc ccttctgtga
    13741 atgtttcacg tgaaacattc acagaataag aagcccataa aggtcttcta ctttttctgt
    13801 catgtaggat tgagcatctg ttattccctg attataatag tgtttcccaa ttttgtctgt
    13861 tataaaatca agaatcattt cggcggcaag ccccccaatt tcctcttccc gctctgtagc
    13921 gaaataatca cgaatttctg ttattgcctc tcttttttgt tccgctgtca attttatttt
    13981 ctccatttca ctcttccctt cctttttcct atcgtatcgc ttctaagttc tttctgtcaa
    14041 gtttttatca cgatcaaaaa cgaaaagacc gcacagctgt taaactgtgc ggtcttcact
    14101 cattgattga tttgatttaa gctgactttc ttgccgcttt cggaattcta atggtatatt
    14161 ccaagtagtc gtcaaaatct ctgcttaccg caacggcgtc aattcctgct cgcttcatta
    14221 cgctaatcgc gtttgaaacc gtattgataa ataatcgtac atccttaata acaggcattt
    14281 tcctttgtgt ttttttatct tgattcggca atagcatttg ctcgatgagc ttgtccgttt
    14341 cctgaacatt gagtttcttg ctgataatga tacgtaatgc cgattcccgt actccgtcat
    14401 ccaatctaag cagcgcgcgc gcgtgccttt cggtcatttc atgatcaaga atcatttttc
    14461 tttcgccgtt ggtcagccgt aaaagccgaa gcttatttgc aattgttgat tgctttttcc
    14521 cgagtctatg ggcaatttcc tcttgtgtca gcccacatgt atcaataagt ttcgcgattc
    14581 cgtccgcttc ttcaaaaaag ttcagatcct gacgttgaag attttcaatc aggcacataa
    14641 ccgccgactg tttatcatta agactgactt caatacaggg tacttgagtg aaaccggcca
    14701 tcaatgacgc ccttaagcgc ctctcccccg caacaagctc aaaattgcca tcttccaacg
    14761 gccttaccga taaaggctgc aaaataccat tgtgccttat gctctcggct aaacccatca
    14821 gtgcttcgtg ctcaaactgt cgtctgggtt gattcggatt tggtttgatt ttaccgatag
    14881 ggaccgaaat caccctgtta ttattcttcg cctttatcgt aaaacccagc atgacattca
    14941 ctcctaaccg tattttttca tcagcttcat cataacacga ttaggaaatt atttccagtc
    15001 tttttcatcg tcttttttcg tcatttttct catctatctg ccttgttttg cggctataac
    15061 ggcattttgt tgattttagc ctgttgtctt gggtatcgct gaggtgttgg tttgattttt
    15121 cgaatagcga tgatcgaacg tttcatatct gtaagcggca aagttatttc atccgccgac
    15181 tcaatctcgc ctccaagcag ttttaccgct ttttgaatgt ccgacatttc ttcccggtaa
    15241 tccggccctt tcattgccag aaacttaccg ccttttttaa ccaacggcag acaatactcc
    15301 agtaattttg aaagttgtgc cactgctctt gaaaccgcaa tgtcatactg ttcccggtgt
    15361 tctttcattc tgcctaattc ttctgccctg ccatgaatgc attgatattg ggtaatctcc
    15421 agttttggca ttaactgctc aataaagttt aaccgtttca ttagactgtc aagacaggtt
    15481 agaaaaatcc gattatcgga tattaaaaga ggaatacccg ggaaaccggc gccacatcca
    15541 atgtcgatca ggtcaacttt cgaaccctcc agatagggcc tgactgtcag actgtcgaca
    15601 aaatgctttg taatgatttc tccgggctcg gtaatggcgg tcaggttcat ccgttgattc
    15661 caagactgca attcttccat aaatatagaa aatctctcaa ttgccctgtt atctaaatcg
    15721 acctttagcg cttcggctga ccgaatcagt gtacctttta attcctcgtt catgaaattg
    15781 tcctccaatt tatgtttcac gtgaaacaat tactactgac cgacgccgga aaggtttgct
    15841 tcgagataaa tcagcagcac agcgatgtct gcggggttta cacccgagat tcttgacgcc
    15901 tgcccaatat tctcaggcct gatcctttta agcttgtcac gagcctccag tctaagccct
    15961 cggatattgt caaaatcaag gctctttgga atctttttga tctcaagcct ttgcatttct
    16021 ttaatctgtg tcatttgtct cttaatataa ccttcatact tcagttgtat ttccacctgt
    16081 tctgttaccg gctcgggcaa agctttgcgc tcaaggtcat aatccgtcaa tgaatcataa
    16141 tcgatctgtg gtcttttcaa taactccgac aggcggatgc cggacgataa aggagctgta
    16201 tgtctggagc aaagaaattc attgatttga tgcgacggag gtacattggt cttttcaagt
    16261 ctttcttttt cctgacggat taggttcatc ttttcctgaa atttccggta tctctgttcc
    16321 gctatcaatc cgattttata tccggtcgga gtcaaacgct catccgcatt atcctgcctg
    16381 agaaccagac ggtattcgga tctggatgtc atcatccgat aaggttcatt ggttccttta
    16441 attacaaggt catcaatcag tgtcccgatg taagactcag aacgatctaa tacgaggggc
    16501 tctcttcctc caacctccaa tgccgcatta atgcccgcaa cgagcccctg cgcagcagct
    16561 tcttcatatc cggatgtccc gttgaattgc ccggcgccga acaggccaat tatcttcttt
    16621 atttcgagag tatgtttgag ttcaagagga ttcacacagt cgtattcaat cgcgtaggcg
    16681 gttcgcatca ttttgatatt ttcaaaacca ctgattgtct gtaaaaattt ctgttgcact
    16741 tcttccggca aagaagagga cattccgccg agatacatct cctcggtatc aatgcccaat
    16801 ggctccacaa aaatttgatg gcgctctttg tcggcaaacc gaacgacctt atcttcaata
    16861 ctcgggcaat aacgcggccc gaccccttca attcttccgg aatacaacgg tgatctcgaa
    16921 agattttctc ttatcacctc atgtgtctga ttattggtat acgcgatatg gcaaacaacc
    16981 tgatttgaaa attcgccttc cgtctcaaaa gaaaaaggaa cgactttttc atcaccctgc
    17041 tgctcttcca gcttatcaaa attaatgctg ctcctgttaa cacgagccgg tgttcccgtt
    17101 ttaaaccgca tcatttcaat acccaggcgt ttcaggcagt ccgaaagagc gtcggctcca
    17161 aacatcccgt cagggccgcc gtcataagag atctctccga tatgaatctt tcctttaagg
    17221 taggtcccgg ttgctaaaat aaccgctttg actttataga ctgccccaag tttggtaaca
    17281 acactctcca cttttccgtc tttgaccctg acatctatga cttccgcctg tttgagatca
    17341 agtccttttt gcagttcaag cgtatgcttc atcactttct gatattctct tcgatcactc
    17401 tgcactctga gagaatgaac cgccgggccc tttcccctgt ttaacattct gctttgcaaa
    17461 aaagtttgat ccgctgcctt gcccatttgt ccgccaagag catcaatttc tctgacaaga
    17521 tgcccttttg cagatccccc aatcgaagga ttacaaggca tattgccaac cgcatcaaga
    17581 ttaatcgtaa atattaccgt tttacatcca aggcgggcgg ccgccagcgc ggcctcgatt
    17641 cctgcatgtc cggctcctat taccgcaaca tcataatttc ccgcttgata ttccacaaca
    17701 atcgtccttc ctgttgaaaa ctattttccc acacaaaact tttcgaacac gcgctgtatg
    17761 atctcatcgg aggccttctc accggtgagc gctgataaac agccaattgc ctcatcaatt
    17821 cctattgaaa tggcgtcaag agtcattccg ctttctaatg caaagagcgc ttcttccaat
    17881 cccttttccg catcatttac cgattggtac tgacgttcat ttgcaagaat tcctgctccc
    17941 aaatccagct gatttgttcc gataatttct gctgcaacct tttctaattc cttgataccc
    18001 tcatgcgttt ttgcagatat atatactgta tgttgtattt tatttaaaat atacttatca
    18061 tcaatttttt tcggcaaatc cgctttattg attatggcaa ccgtcagctt atttgatata
    18121 aagtcaataa gcttcatatc ctcctcgtca agttcccggg aagaatcaaa aacagctaat
    18181 atcaaatcag cattttcaag tttgcttttc gcccgttcaa caccgatttt ttcaatcgga
    18241 tcatccgtct gccttaaacc ggcagtatcc gagaggttta aaatcaaatc gcccaacact
    18301 acccggccct ccacaatgtc ccgcgttgtt cccggtattt cggtaacgat acttttttca
    18361 tatccggaaa gaagattcat caatgtcgac tttcctacat tagggcttcc cgtgattacg
    18421 gtatcgatac cctcccgaat cattttaccc gtttcatagg ttgcaatcag cctcttcatc
    18481 ttatccgcta tttgcttgat ggatgatttt aaattgtctt cctgcaatcc ttccatctca
    18541 tcatcaggga aatccatcca agccgaaata tctgaagaaa tttcgatcag atgactctta
    18601 atcagcatta tggaagaaaa caatgcaccc tcatgctgca ttcttgctgc atttaccgcc
    18661 attttactct gggcattaat gagatccata acagattccg ctttcgtcag gtccaatttt
    18721 ccattcaaaa acgctctttt cgtaaattct cccggctcgg caagtcttgc tccagccaaa
    18781 atcgctgctt ttagaaccat cctcattacc attattccgc catgacagga taattccaca
    18841 acattctcgc cggtataact tttcggagca tgaaaaacaa gtgcgatacc ttcgtcaatt
    18901 tctttctcac cgtctataat ttttccatat gcagccgtat agggcttcat ttcaactatt
    18961 tctttgctat tctttgaccg gaatatcttc tgagcaattt caaaagcgtt ttcccccgat
    19021 attctgacga ttccgattcc tccggccgcc accggtgttg cgattgctgc aatggtttcc
    19081 actgttcctt catctccatt ccatataaga caaatccggg ggtcttctcc cccggatcct
    19141 attgtctatc ccttatatta tgatcgaaac attttctgcg gcgctcttga agcattctcc
    19201 gctccaatga ccactctccg atttggctct tcacctaccg accaggaagc tgctcccctg
    19261 atttcctgaa cagccgtatg aatgattctt ctttcatatg gattcattgg ctcaagcgtt
    19321 gtcgttctct tcgttcgaat tgaatttaac gcaatcctct tggcgagagc catcagcgtt
    19381 ttttctcttt tttcccggta atttccggtg tctattgtaa tccttttata ttgttcttcg
    19441 cctttatttg ccacaagtga taccaggtat tgaagcgaat caagcgtttc ccctctgtgg
    19501 ccgataataa ttccgagccc ttcacccgcc aaatcaaaat ttattccttt ttctgtttca
    19561 gttgattcga tgatgactcc cggcaattcc attttttcaa gaatctgttt gaggtaattt
    19621 cgcgccttgt cttctacgcc ttcattataa actaccttga cctttgcgtc ggaggcacca
    19681 agtaatccca aaagtctttt tgaaggcagc tcaagaattt caaactcgac ctgttctctt
    19741 tcaacaccaa ggctctcaca agcaattcta attgcttcct caactgttct tcccgtgcct
    19801 atcgcttcgt atttcacttg ccggcacccc ctgtttttta tttgtcttga ccgccgcgct
    19861 tttccatttt ccgcgaagcg gcaagtcttt ttctattttc ttcctttatc tgcttttttg
    19921 atttcatttc ctgaggaagt gcttttgcgg actttttctt ttcttcatcg ggacctgctt
    19981 cggctgtatc ggaaattccg cttggcttat cgcttccctc ggttacttta tcgtcacttt
    20041 tatttgcggc aatggccgct tttctcgcgg cggctcgggc gtcttttctt ttcttcgctg
    20101 caatttcagc ctgttccgcc agcttcttcg gattatagaa tatgtttatt acataaacct
    20161 gcacaatcat aaacaagttt gttatgatcc agtagaaacc gattcccgcc ggaagctgca
    20221 gcgaaaaaac agtggacatg atgggcataa gaaccatcat cattttgttg ttcatcggat
    20281 tattctgggt agtcggggtc gccatcattt tcatattgac ccacatgtaa aggaagttgg
    20341 tgacataaca caaaattggt ataagtatca gaatattaag gctgagtgac ggggttttgg
    20401 ataaatcaac tcccaaaaag ttaaaatcaa gcgccatgat tttatggggt aaaaaggata
    20461 atttatccat attgccgttc atggcctgcg cggcatatat ctcaatcttc gattgatcac
    20521 cggaattaat tgcatgatga agcattgtct gtatctgtgg agtaagcgtt gccactacct
    20581 tattgacctg aacggcggtt aaaccgagga tataagtcaa aggtttataa ataacaccga
    20641 aaagtccata aataatcgga agctgaatta aaagcggaag acaaccggca aacggactgt
    20701 atccttcttc ctgataaagc ttctgaagtt cttcggcctg tttcgtttta tccttaatct
    20761 ttttttggat ttccgccatt ttcggctgca tccggatcat ttccgccgat gacttctgct
    20821 gcttgattgc aagcggaatc atcagcactc ttgtaaccag cgtaaacaga atcagagcga
    20881 ttccgtaatt tcttccgacc tgataacaag cacccagcaa aaagccgaga ggccggtaaa
    20941 ttaccatatt aaaaaagttt ccgatatcgg caaataaatt cattttgacc tccatgcgcg
    21001 gatacgcacc cttggtattc cattatttga cgcatggaag cgattctggc catgcgctta
    21061 attgcgtttg caaatttttt cggatggata aataagcgga accggatcaa cgcctcccgg
    21121 atgaaacgga tgacatttca aaattcttct cattgaaaga agccccccgc gcaaaacccc
    21181 gaaacgggat attgcggtga ttgcataatt cgaacaagtc ggataaaatc tgcagctcgg
    21241 gggttttaaa ggtgatagat acctttgata aaaccgtatt aaacggatga aaacgccttt
    21301 cactttttcc agactcccgc ctgaatgaac aggcccttca atgtcttcat catttgccat
    21361 gatttaaggt tgaccgctct gtttctcgcg acaaatacga aatcccatcc tgtttttgtc
    21421 tgagtttcca caccgctgta cgcttccctg atgatgcgtt taaccctgtt tcttataacc
    21481 gcttttccca actttttccc tgttgttatt ccaattctgt tatattgctt tttgttttta
    21541 agcatataaa caacaacgac gggagaaaca taggatctgc cctttttata aagccttcta
    21601 aattccctgt tttcttttaa cgaaactgtt tgttgcatat catcatcctg aaagcaccgc
    21661 aaaacacccg gaattcattt ccagcagtgt tttaacggca gatcggcggc ggtttaaccg
    21721 ccccgccgcc ataaaataaa gaccacacat aaggtggcct tcggattagt aggtcaactg
    21781 cttcctaccc ttggccctgc ggcgggcaag aaccttgcgt ccgtttttcg tggccattct
    21841 tttgcggaac ccatgttcct tatttctgtg tatctttttg ggctgatatg ttcttatcat
    21901 tttaagcccc cctttcgagg tcaaccttgc actgtaacat tatataataa cgccccatca
    21961 gtgtcaacat ttttttacgg ttttctgccc ctgcttcaat tcaatcgttt tcagccttct
    22021 atttgattat aatgtaactt aatttttatt tcccatgttt ttctatttta cacccatttc
    22081 attcatttat tcaactcttt tcttttgtct gcatgaaaag tcgtcaaaaa tcaaataagt
    22141 tgataaaaca acgtctttgt gttatgataa tatatattta tacgcctgtt tatatgtttt
    22201 ttttctattg tgtcttaaaa ttcgtatttc atatgccatc aggtgaaaat cagacggtgt
    22261 tccacagctt ttaaccggtt ttcatctatc ttttcacagg ttttcaacat tttcaacaag
    22321 gttttcaaca gctattaaaa ctgtggaggt ttgacgttaa tgcagtcctt taaggaagtt
    22381 tggtcccagg tttgccaata ttgcaaagga aaaataagcg atgttgcatt taagacgtgg
    22441 atatattgca tggaacctgt tgaactgaaa agcgatgaag ccgtattttt tgtgcgttcc
    22501 gattttcagc gcaatatcat ttttgatcgt tacaaggagc tgctgatcga tggctttgaa
    22561 agcgttcttg gctttaaagt cgacattaac atcgttacgg aagaggataa gccaaacggt
    22621 tatcgctccc aatacgattt tgtgattacg cagaacccgg aagatatcga aaagtcctat
    22681 gcaagcgcag attatgaata cacgtttgat acttttatcg ttggttcttc caacaaattt
    22741 gctcacgctg cttctcttgc cgtcgcatca aatcctgcga tggcctataa tcctctattt
    22801 atttatggcg gatcagggct tggcaagaca catcttcttc atgcaatcaa gtatgaaatt
    22861 gaaaaaaacg accccggcag caacattatt tacattaaag gcgacgattt taccaacgaa
    22921 ctggtcgaat ccatccgcca gaacagtcag attcagttcc gtaataaata ccggcagtca
    22981 accgttcttt tagtggatga tattcagttt attgccggaa aagaaggaac acaggaagag
    23041 ttttttcata cctttaatac gctatatgaa gcaaaaaagc agattgttct gacttcagac
    23101 cggcctccaa aagaaattca aacattggaa gaccgtctga gaacccgctt tgaatggggg
    23161 cttctagccg atgttcagcc tcccgatttt gaaacaagac tggcaatcat taaaagaaaa
    23221 gcggaattgc tcggaatatc gattccggac gaagtttcgg aatacattgc aaacaagctt
    23281 aaaaacaaca tccgtcagct ggaaggcgct gtgaaaaaaa tgaaagcgta caatcttctg
    23341 gccggcgacc ccccctctat tgcaattgcc caaaacgcca tccgcgatat tttaaacgat
    23401 aaccagccgg ttcccgtcac cgtagaccgc atcatcggcg aagtatcgag aacattcggc
    23461 gtaacaggcg acgaactcag agggaaaatg cgttccggca atatttcatc cgctcgtcag
    23521 gtcgccatgt atattgtccg ggatattacg caaatgtcga cttcttccat tggcgatgaa
    23581 ttcggcggaa gagatcattc tactgttgtt tacgccattc aaaaggttga agacgacatg
    23641 tcctccaacc agagattccg ggctaccgtt caggacatta taaaaaacat cagagatcac
    23701 tgacttctac atatctttat caacaactac atttttcttt tttccaccac tattcctgca
    23761 agatattcat ccctcttctc ttttcaccga atcttcaact ttttatccac tttcaaccga
    23821 aaattttcaa cctgctgttt aaaagaaaaa ccaccctgtt gaaaagcttc cgtcttctca
    23881 ctcccgttca acaatgagca aaatcgctca tccctgtcac tgtctctaaa acaagacctt
    23941 ttcaactttt caaccgtccc taatactact actgtttatt tatccttatt attctttata
    24001 gaaaggacgt ggtgcgcatc atggaacttt cctgcaataa aaaagatctg gccgaagcgg
    24061 tatctaccgt tcaaagagct gtttctacaa aatcttcact tcccgcgctg gaaggaatat
    24121 tgataaaagc gtctgaacat gttctaacgc tttccgctta cgatcttgag cttggtatct
    24181 ccaaatcgat cagcgccagc gtttcccagc cgggcgaaat tgttttaaac gcccgtattt
    24241 ttgccgatat ggtaaaaaaa cttccggccg agcaggtaga attgaaaaca gacgaaaagc
    24301 ttttaaccac cgttaacagc gggaactccg aatttacgtt tctcggtatt ccttcttccg
    24361 aatttcccga aattccttcc ctgaccgatg gtacaatgtt tacccttccc caaagtctgc
    24421 tttccagtat gattcgccag acattgtttg cggtttcttc cgacgatacg aaaccgattc
    24481 acacagggtc tctttttgaa atagacaacg gcgtttttaa tatcgtttct gttgacggct
    24541 atcgtctcgc
//
LOCUS       DATALY010000018         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_177419,
            whole genome shotgun sequence.
ACCESSION   DATALY010000018 DATALY010000000
VERSION     DATALY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177419"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VHR42_00620"
     CDS             <1..256
                     /locus_tag="VHR42_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025724.1"
                     /translation="DKRKSGLFSIHITSDLDKFEDYITLTDHGKILFSRSKDDLLEGY
                     ALYSVKSQLLGLFHAPPAESNSRFFFGYNENREQIAVYPV"
     gene            complement(509..1438)
                     /locus_tag="VHR42_00625"
     CDS             complement(509..1438)
                     /locus_tag="VHR42_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEX3025725.1"
                     /translation="MERKLQIFNLVMWILIAAALILLMVFFMFRGPWYQRFNLFSWKP
                     SAAAAETYDKTFASADITKICVGSGAADITVKQSDTGDIRVIHRSSKDIKDSSFSVQT
                     ENGTLNIQDNNDFSGIHLFMFSSWTEQIEIDLPRSFGKAFSIENGSGDVIFTGGNTFA
                     ELSIQTGSGDFTADNIKAQSFSLKTGSGDIHVENLNAAHYDAESGSGDIRFNDLAGCG
                     RLKASSGEIEARCSALTGDLTASTGSGDVKLSLPENTSEKIQASTGSGDINSSLPLSY
                     TGWDKNTATGTVGSGPFQNVEIHTGSGDINLNK"
     gene            complement(1445..>1652)
                     /locus_tag="VHR42_00630"
     CDS             complement(1445..>1652)
                     /locus_tag="VHR42_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025726.1"
                     /translation="VEEFKEWHSSNSDKVRIRKSISSVLWPLVVITYLLVSFFTGQWG
                     ITWIIFLIGVAIENIIRAVFELKR"
BASE COUNT          421 a          411 c          388 g          432 t
ORIGIN      
        1 agataaacgg aagtctggcc ttttttccat ccatatcaca agtgatcttg acaaatttga
       61 ggattatatc acgctgactg accacggaaa aatcctcttt tcacgaagca aggacgattt
      121 gcttgaggga tacgctcttt acagtgtgaa gtcgcaattg ctcggcttgt tccatgcccc
      181 acctgccgag agtaacagcc gttttttctt cgggtataac gagaatcggg agcagatagc
      241 cgtttaccct gtataggtaa tttcttctgc catggtcaaa gcgcctcctt aaacacgctc
      301 cagatggaac tggaaacgca gctccacgaa ttgctgaacg cctgactgag cgccgagctc
      361 aaagggcctg ttagccaatc gaccaaataa tccaccccct aaaatacagc aaggccggga
      421 tgtcatacaa aaacatcccg gccttctcag tttcatagta tttatttttg attcttataa
      481 aaaatgtttc atactttctt tcgggaacct atttgttgag attgatatcg cccgagcccg
      541 tatggatttc gacattttgg aacgggcctg agccgacggt tcctgtcgcg gtgtttttgt
      601 cccagccggt ataagaaaga ggtaggcttg agttgatgtc tcccgaaccg gtggacgcct
      661 gaattttttc ggatgtattt tcaggaagac tcaacttgac gtcacccgac ccggttgaag
      721 ccgtaaggtc gcccgttaac gcggagcagc gcgcctcgat ttccccggaa gaagccttca
      781 gcctgccgca gccggcaaga tcgttgaacc ggatatcgcc tgaccctgat tcagcgtcgt
      841 aatgggcagc attgagattt tccacatgaa tgtcgcccga cccggttttc aggctgaagc
      901 tctgcgcttt tatattgtcc gcggtaaaat cgcccgatcc ggtttgaatc gatagttccg
      961 caaaagtgtt gccacctgtg aaaatgacgt cgccggaacc gttttcaatc gaaaaggcct
     1021 tgccgaagga gcggggaagg tcaatttcaa tttgctccgt ccagctggaa aacatgaaca
     1081 gatggatgcc ggaaaaatcg ttgttgtcct gtatgttcag cgtgccgttt tcagtctgta
     1141 cggaaaagct gctgtctttt atatcttttg aagagcgatg gatcacgcgg atgtcgcctg
     1201 tatcggactg cttcacggta atatcggccg caccgcttcc aacgcaaatt tttgtaatat
     1261 ccgcggacgc aaaagtctta tcgtaggttt cagctgccgc agcactcggt ttccagctga
     1321 acaggttaaa tcgctggtac catgggccgc ggaacataaa gaagaccatc agcaaaatca
     1381 gagccgccgc gatcagaatc cacatgacga gattgaatat ctgcagtttt ctttccattc
     1441 taaattacct ctttagctca aagacggctc tgatgatatt ttcaatcgct acgccgatca
     1501 gaaaaataat ccatgtgatg ccccactggc ctgtaaaaaa gcttacaagc agataggtaa
     1561 tcacaacaag gggccacagc acggaggaaa tagatttacg gatccggact ttatcggaat
     1621 tgctgctgtg ccattccttg aattcctcta cc
//
LOCUS       DATALY010000019         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_177589,
            whole genome shotgun sequence.
ACCESSION   DATALY010000019 DATALY010000000
VERSION     DATALY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177589"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..617)
                     /locus_tag="VHR42_00635"
     CDS             complement(237..617)
                     /locus_tag="VHR42_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025727.1"
                     /translation="MDIDRCAQITVGDEQYKLVLTTRATKEIAKRYGGLEDLGEKLMK
                     SENFEMALDEIVWLIVLLANQSVLIHNLQNAEKWELLTEEAVELLTSPLDLADYKNAI
                     MEAMMKGTKRYVESEDSEKNAEVG"
     gene            complement(632..1219)
                     /locus_tag="VHR42_00640"
     CDS             complement(632..1219)
                     /locus_tag="VHR42_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HEX3025728.1"
                     /translation="MATIGLDKLYYSKITEGTGGDETYAAPVLLAKALKCDLSVDLAE
                     ATLYADDAAQTVVKEFKSGKLSLGVDDIGVSAAEDLTGAVLDKNGVLISASEDGGGPV
                     AIGFRAKKANGKYRYFWLYRVVFGIPATNLETKGDSIKFSTPTIEGTITQRNKVDGNG
                     KHPWKSEANENDSGVLAATISGWFTQVYEPDYTQA"
     gene            complement(1220..1564)
                     /locus_tag="VHR42_00645"
     CDS             complement(1220..1564)
                     /locus_tag="VHR42_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025729.1"
                     /translation="MSILEELNTLLTGIGLSVETGMFSGAPPDEYLVITPLADTFEGY
                     ADDLPQQEIQEVRLSLFSKGSYTARKNQIVRSLLENNFTVMDRRYIGHEDDTNFHQYA
                     IDVAKEYEMGDE"
     gene            complement(1561..>1887)
                     /locus_tag="VHR42_00650"
     CDS             complement(1561..>1887)
                     /locus_tag="VHR42_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEX3025730.1"
                     /translation="KVKSNLQAVVGSNTKYKSRSTGELVDALGVTSPKQDRDGNFNIK
                     IGFAEPRKNTGKAQNASNAMLASILEYGKTGQPPKPFLKPAKTATKKPCIQAMTEALE
                     KEIENL"
BASE COUNT          406 a          504 c          431 g          546 t
ORIGIN      
        1 aagttaatga attattccta aacaaagtat atgtttttgc ctgtatcggc atgtcagcga
       61 ttaaataata tcgtcaataa agtattcccg tttcggcttt gccaacccga gaaattgctt
      121 gtggatttcc cattggtcga gcaggtcacc catcggcatg agccacacgt cggatttgga
      181 tcggtgaagt tgggtgacgc cataataaat cagtcgggca aacagttctt cgtctgttac
      241 ccgacctccg cgtttttttc ggagtcctcg ctttcaacat aacgctttgt gcccttcatc
      301 atagcttcca taatggcgtt tttgtaatcc gctaaatcca gtggtgaagt gaggagctcg
      361 actgcttctt cggtgagcag ttcccatttt tccgcgttct gcaggttgtg aattaaaaca
      421 ctctggttcg ccagaagcac aatcagccac acgatctcat ctagagccat ctcaaaattc
      481 tcggacttca taagcttttc gccgaggtct tccagcccgc catagcgctt ggcaatttct
      541 tttgtcgcgc gggtggtgag gacaagctta tattgctcgt ccccaaccgt aatttgagcg
      601 catcgatcaa tatccataaa ctaattctcc ttcatgcctg cgtgtaatcc ggctcataaa
      661 cctgcgtaaa ccagccggag atagtggcgg ccagaacgcc ggaatcattc tcgttcgctt
      721 cggacttcca cgggtgtttt ccgttgccat ccaccttgtt acgctgggta attgtaccct
      781 caatggtagg cgtggaaaat ttgatgctgt cgcccttggt ttcgaggttt gttgcgggaa
      841 tcccgaacac gacccggtac agccagaaat accgatattt tccgttcgct tttttcgccc
      901 ggaagccaat cgcaacaggt ccgccaccat cctcacttgc ggaaatcagc acgccgtttt
      961 tgtcaaggac agcaccggtc aaatcttccg ccgccgaaac gccgatgtca tcgacgccca
     1021 gcgaaagctt cccggatttg aattccttta caacggtctg cgccgcgtcg tccgcataca
     1081 gcgtcgcttc cgcaagatca acggaaaggt cgcatttcag cgcttttgcc aatagtaccg
     1141 gtgcggcgta tgtctcgtcg ccgccagtgc cttctgtgat tttcgaataa tacagcttat
     1201 ccaagccgat ggtcgccact tattcatccc ccatttcata ttcttttgcc acatctatgg
     1261 catactggtg aaaattcgta tcatcttcat gtccgatata ccggcggtcc atcacggtaa
     1321 aattgttctc cagcagggac cggacgattt gattttttcg cgccgtgtaa ctgcccttgg
     1381 aaaacaggga cagccggact tcctgtattt cctgctgagg caggtcatcg gcataacctt
     1441 caaaagtgtc tgcaagcgga gtaatcacca gatattcgtc cggtggagcg ccgctgaaca
     1501 tgccggtttc tacggaaagt ccgattccgg tcagaagtgt attgagttct tccaagatgc
     1561 tcataaattt tcaatttcct tttccagagc ctcggtcata gcctgaatgc acggcttttt
     1621 tgtcgccgtt tttgccggtt tcaggaacgg tttcggcggt tggccggttt tcccatattc
     1681 cagaatgctg gccaacatgg cgttgctggc gttttgggct tttcccgtat tttttctggg
     1741 ttccgcaaaa ccaattttaa tgttgaaatt cccgtcccgg tcctgttttg gtgaggttac
     1801 gcccaacgca tcaaccaatt cgccggtgga gcgggactta tattttgtat tacttccgac
     1861 caccgcctgc agattgcttt tcacctt
//
LOCUS       DATALY010000020         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_436663,
            whole genome shotgun sequence.
ACCESSION   DATALY010000020 DATALY010000000
VERSION     DATALY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436663"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VHR42_00655"
     CDS             <1..335
                     /locus_tag="VHR42_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEX3025731.1"
                     /translation="RYICGMHGIKINIEDYADRISAIYRELVRQNKALEINTSGLRQQ
                     FGTTMPDLWGLELYKKAGGEMITVGSDAHTAKDVGTGIMEGIALAKQAGFSDVYLFRD
                     RKPVAQKI"
     gene            554..1807
                     /locus_tag="VHR42_00660"
     CDS             554..1807
                     /locus_tag="VHR42_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3025732.1"
                     /translation="MNILYNMVINFWILLNIATTTYCLYHISLAIAGFRKKKPFSGEL
                     KNHRFAAIIAARNEESVIGSLIESIRLQNYPKELIDIIVVADNCDDKTAEIAQKCGAI
                     VYKRFNKAEVGKGFVLKFVFSKIFEERDVYDAFCIIDADNIVDRNFFLHMNQAICEGY
                     RVAQGYRDMKNPADTWISGGHSLFYWMENRFFNHSRSILGLSATINGTGFMVATDLIK
                     EIGFQTYTMTEDIEFTIHCVLHGYKVGYVPEARVYDEQPITFAQSMRQRIRWTNGLMQ
                     CFMRYTGVFAKRLWQKFDWPILDMLMYLFAVPTMVIGAVASVIYAILAAVRIFDPMGV
                     FVNSLLLLVGSVAAFWLVGWLTVTLEKKDVKKMAWSVIAYPVFNLLWILIYAICIFKK
                     KIDWKPITHVRNISIHEMESSKTIK"
     gene            complement(1954..3228)
                     /gene="uraA"
                     /locus_tag="VHR42_00665"
     CDS             complement(1954..3228)
                     /gene="uraA"
                     /locus_tag="VHR42_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEX3025733.1"
                     /translation="MQSKIIQVEERVPLMKGIPLSLQHMFAMFGASVVVPLTFHINPA
                     IVLLMNGVGTLLFILITKGRAPAYLGSSFAFISPVLMILGPHFTDYRLALGGFVAVGA
                     VFCLVAILIRFFGTKWIDVMLPPAAMGAVIALIGLELAGTAAQTGGLILTETYKALDP
                     RYVAVFLVTLLVAVFGSVLFRKFFAVIPILIAIITGYVLACLLHIVDFSLVAKASWFA
                     LPNFQTPVFNLNAILIVLPASLVVISEHIGHQLVTSNIIGRDLIKDPGLHRSLLADGL
                     STIISGFTGSVPTTTYGENIGVMAITHVYSVWVIGGAAVISATIAFIGKVSAFIQTIP
                     GPVIGGVSFLLYGMIASSGIRLLVESKVDYSRSRNLALTSVVLVTGLSGAYIPLGSIQ
                     LKGMALATIVGMALSLLFYLIDKFKLANDIAE"
     gene            complement(3375..>3983)
                     /locus_tag="VHR42_00670"
     CDS             complement(3375..>3983)
                     /locus_tag="VHR42_00670"
                     /inference="COORDINATES: protein motif:HMM:NF023166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HEX3025734.1"
                     /translation="PVSSKISDFYRQTALDYYNYAMDKLYHTALDDYHERKNNKIPFH
                     TYTAMMTYEVPLNLNGLLSITYDIYEFTGGAHGNTVKHADTWNMLSGNRLDLSDYFIS
                     PSYKSIIFHNITAQISDQIAQGNNYYFDNYQTNVFKYFNENNYYLTPDGFAFFYPLYT
                     IAPYVAGIVTFIVPYEKFDGYYTPEMKNWIKALPDSIKTREK"
BASE COUNT         1108 a          859 c         1004 g         1012 t
ORIGIN      
        1 ttcgttatat ctgcggaatg cacggcatta aaattaacat agaagattat gctgaccgga
       61 tttctgcaat ttaccgtgaa ctcgttcgtc agaataaagc tttggagatc aatacttccg
      121 gtctccggca gcagtttggt acgacaatgc ccgacctgtg ggggctggag ctttacaaaa
      181 aagcgggcgg cgagatgatc accgtgggat cggacgccca taccgcgaag gatgtcggca
      241 ccggtatcat ggaaggtatc gcgcttgcaa aacaggcagg cttttcggat gtttatcttt
      301 tccgcgaccg gaaacctgtc gcgcagaaaa tctgatcatt caaaactcct tccaaaaata
      361 tggacatttt tcatataatc tgataatatt attgaaacag acttttcgtt tccggttgcg
      421 cacagctccg gcggcttttt aaattgaccg gatcttaaaa atttgtaaat actattttca
      481 aatgacgata aaattacccg ggctggctgc ccaagtgaga gaccggcctc cgaataatac
      541 cgaggtgatg tggatgaata ttctatataa tatggttatt aatttttgga tactgttaaa
      601 tattgcgact acaacctact gtttatatca tatttccctt gccatcgcgg gattccgcaa
      661 aaagaaaccg ttttccggtg aactcaaaaa ccatcggttt gcggcgatta ttgccgcaag
      721 aaacgaagaa tcggtcatcg ggagcctgat tgagagcata cggcttcaaa attatccgaa
      781 agagttgatc gatatcatcg tggtcgccga caactgtgac gataaaacag cggaaatcgc
      841 gcaaaaatgc ggcgcaatcg tttataaaag gtttaacaag gcggaagttg gaaaaggttt
      901 cgtcttaaag tttgtttttt ccaaaatatt tgaggaacgc gacgtctatg acgcattctg
      961 tatcatagat gctgataata tagtagatag aaatttcttt ctacatatga atcaggcaat
     1021 ctgtgagggc tatcgggtcg ctcagggtta ccgggatatg aaaaatcccg ccgatacctg
     1081 gatctcgggc gggcattcgc tgttttactg gatggaaaac cggttcttca atcattcaag
     1141 aagtattctg ggtttatccg ccacgattaa cggaacaggt tttatggtgg caaccgacct
     1201 gatcaaggag atcgggttcc agacttacac catgactgag gatattgaat ttacgattca
     1261 ttgtgtgctt catggctaca aagtgggtta tgtccccgag gccagggttt acgacgagca
     1321 gccgataacg ttcgctcagt cgatgcgcca gcggattcga tggaccaacg ggctgatgca
     1381 gtgttttatg agatatacag gcgtttttgc caagcggcta tggcaaaagt ttgattggcc
     1441 gatcttagat atgctgatgt atcttttcgc ggttccgacg atggtgatcg gcgctgtcgc
     1501 aagtgtaatc tacgcgattc tggcggctgt gcggattttt gatccgatgg gtgtctttgt
     1561 caacagtctc ctgcttctgg ttggctctgt cgctgcattt tggctggtcg gctggctgac
     1621 tgtcacgctc gaaaagaaag atgttaaaaa gatggcttgg tcggtgatcg cctatcctgt
     1681 ttttaatctg ctttggattt taatttacgc gatctgcata ttcaagaaaa agatcgactg
     1741 gaagccgatt acgcatgtgc gaaatatcag tatccacgag atggagtcgt cgaaaacaat
     1801 caaataggaa ataatccaag atagcagggc cggctttcca caaacgcgga aagccggccc
     1861 tgattttaat ttcagtgatc tttatagata aggctgcggc ccgttgccgt tttgatctct
     1921 gcagccgaac aggcggtgat accgaagccg atgttattca gcgatgtcgt tggccagctt
     1981 aaatttgtcg atcaggtaga agagaagact gagggccatg ccgacaattg tggccaaagc
     2041 cattccttta agctgaattg atccaagagg aatgtaagcg ccgctcaggc ctgttaccag
     2101 aacgactgaa gtgagtgcaa gattgcggga acggctgtaa tccactttgg attccaccag
     2161 aagccggata cccgaggaag cgatcattcc gtagagaaga aagctgactc cgccgataac
     2221 cgggccgggg atggtctgaa tgaatgcgga aacctttcca ataaaggcga tggtggcgga
     2281 aattaccgcg gcgccgccaa tcacccagac gctgtaaaca tgcgtgattg ccataacgcc
     2341 gatgttttca ccgtaggtcg tggtcggaac cgagccggtg aaaccggaga tgatggtgga
     2401 aaggccgtcg gcaagcaaag aacgatgaag tccggggtct ttaattaaat cccggcctat
     2461 aatattgctt gtgaccagct gatggccgat gtgctcggag atgacaacca gcgaagcggg
     2521 taaaacaatc agaatcgcat tcaaattgaa aaccggtgtc tggaagttgg gaagggcgaa
     2581 ccagctggct tttgcaacca ggctgaaatc gacgatatga agcagacagg caagcacata
     2641 gccggtaata attgcgatca ggattggaat gaccgcgaaa aatttgcgga ataagaccga
     2701 accgaaaacc gcgacaagca gcgtgaccag aaatacagcg acatatctcg ggtcaagcgc
     2761 cttatatgtt tcggtcagaa tcaggccgcc ggtctgcgcg gctgttcccg caagctccag
     2821 accgatcagc gcgatgaccg cacccatggc cgccgggggg agcattacgt cgatccattt
     2881 ggttccaaag aagcggatga gtatggcaac caaacaaaat accgctccca ctgcgacaaa
     2941 tccgccgagt gccaagcggt aatccgtaaa atgcggcccg agaatcatta atacgggcga
     3001 gataaatgcg aaacttgaac ccaagtaagc gggagctcta cctttggtga tcagaatgaa
     3061 caggagcgtt cccacaccat tcatcaaaag aacgatagcc gggttaatat ggaaggtcag
     3121 cgggacaaca accgaagcgc caaacatggc aaacatatgc tggagactaa gcggaatccc
     3181 cttcatcagc gggactcttt cctccacctg gataatttta ctctgcacaa attacacctc
     3241 cgagtttttt caatccggca gccgcaagaa tcaatcgtgc tcacacataa gcggcagctt
     3301 tcatttccag tcatttagta tagcataagt ttcgactaat gaaaatgttt tcgctcaaaa
     3361 tcggtcaaat aaattcattt ttcacgggtc ttgatcgaat caggcagggc cttgatccaa
     3421 tttttcattt caggagtata gtatccatca aatttctcat agggaacgat aaaggtgacg
     3481 atgccggcaa cataaggagc aatggtataa agcggataga aaaaggcaaa tccatccggt
     3541 gtcagataat aattattttc attgaaatat ttgaaaacgt tggtctgata gttgtcaaaa
     3601 tagtagttgt tcccctgagc gatctggtcg gagatctgag ccgtgatatt atgaaaaatg
     3661 atgcttttat aagaagggga tataaaatag tcgctcagat ccagacggtt tccgctgagc
     3721 atgttccagg tatcggcatg tttgaccgta ttgccgtgcg cgccgccggt gaactcgtaa
     3781 atgtcatagg tgatgcttaa caggccattg agattaagcg gcacttcata ggtcatcatt
     3841 gcggtgtatg tatggaaggg aatcttattg tttttccttt cgtgataatc atccagcgcg
     3901 gtgtgataaa gcttatccat ggcgtagttg taataatcca gcgctgtttg ccgataaaaa
     3961 tcgctgattt tactggatac cgg
//
LOCUS       DATALY010000021        18855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_3751,
            whole genome shotgun sequence.
ACCESSION   DATALY010000021 DATALY010000000
VERSION     DATALY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18855
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3751"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VHR42_00675"
     CDS             complement(<1..1053)
                     /locus_tag="VHR42_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3025735.1"
                     /translation="MIEYLICAMRNLGRKKIRTILTVSGIMIGVASVIVIGAIGEGGK
                     LAITGQLDQLGINGLDVRSKTTQTGAYSASINDKDVTACLNVKGVQSAMPVVMQSAVS
                     VLRGSQKDSLIWGVSSDAEKIISLKIIHGRMFSQSDVKTGANVCLVDDSFARSAYKRD
                     NISGKKITVFVGTESRELTIIGIVKTGSSLLNSLAGNYIPSFVYMPVSTAKDLKGQVG
                     YDQIAIKTTSDKNTDSIGNKIISKLNQIHGVSDNYVADNIFKQKEKLSNLLSIVTLII
                     SAVGAISLVVAGLGIMTVMTVSVNERTREIGIKKAIGAKKSVVLMEFLFEALLISLIG
                     CLLGIAAGTGISFLASR"
     gene            complement(1050..2141)
                     /locus_tag="VHR42_00680"
     CDS             complement(1050..2141)
                     /locus_tag="VHR42_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3025736.1"
                     /translation="MKKYVILLGVTLAAALLLILIPQLAGSSITTVTAVRIMNGNMKS
                     TISCSGTISSEYTSAVSYGYSMKISGISVAVGDSVKKSQKLMDIDQQATRQGIQSGSV
                     SSQSGSSTVSASSSADSQQSEYLNSLAKQYNLTQYSSSGSASSQSGSSSSASVSQIPS
                     EVCAPISGTVTQISAVCGAFTNPRAAIITISDLSHLEVTAQVEESLITKVKDGQTAYV
                     TGDGFSGCCKGYVARIYPSASQSSSLSGTGSTVAVVVKIEKPVSGLKPNLTANVEIVT
                     SDNPKTIAVPYEAVLQDDSNVEYVFRYRNGRAMRQNVVTGDEYDDCVQVVKGLSQGDE
                     ILLNPPSTLKSGEIVRLKTAKTGSGVSSK"
     gene            complement(2480..3850)
                     /gene="radA"
                     /locus_tag="VHR42_00685"
     CDS             complement(2480..3850)
                     /gene="radA"
                     /locus_tag="VHR42_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX3025737.1"
                     /translation="MAMKSKSIYVCTQCGYESPKWYGKCPGCGEWNTLVEEIREPVKP
                     SAKSHPTASGVSRSVKINELSMENEERYSTGLKELDRVLGGGIVRGSLILLSGDPGIG
                     KSTILLQICGFLGQDKKILYITGEESVRQIKLRAQRLNIDSPNLFLLAETDMENVVQV
                     IYGEKPDIVIVDSIQTMYKRDMSSSAGSVSQVRECTHELLNAAKFTSIPLFVVGHVNK
                     EGAIAGPKVLEHIVDAVLYFEGERNLSYRILRAVKNRYGSTNEIGVFEMTDTGLREVE
                     NPSMMLLSGRPLNISGTSVACVMEGTRPILAEVQALVTSSGYGNPRRMSTGFDYNRMS
                     VLLAVLEKRAGYYFSNADAYLNIVGGLRLDEPAADLAVALSMVSSLKDQPVDPYTVAF
                     GELGLAGEIRAAGRAEARIAEASRLGFKRCILPRHNLNTLQGREKYEIELIGVKSVRE
                     AFEAVI"
     gene            complement(3874..4602)
                     /gene="cwlD"
                     /locus_tag="VHR42_00690"
     CDS             complement(3874..4602)
                     /gene="cwlD"
                     /locus_tag="VHR42_00690"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HEX3025738.1"
                     /translation="MRRQNISGDLLIQVTIVSFVILLVIFVDLTAFRSSRAFGSEDVK
                     TEEKTIIIDAGHGGEDGGAIGYDGVIEKDINLSISLKLKTFFRLAGYQVIMTRDSDKA
                     IYDSGSNTIRQKKTSDLHNRLKLASSYPDALFLSIHQNIYESSRYSGAQVFYSQNNPQ
                     SKTLAEIIQKQIREQLQPTNNRMIKKAEKNLFLLYYAKSPSVMVECGFLSNPTECKNL
                     ENNSYQQKIAFSIFSAVLSYGSGT"
     gene            complement(4670..5188)
                     /locus_tag="VHR42_00695"
     CDS             complement(4670..5188)
                     /locus_tag="VHR42_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025739.1"
                     /translation="MLRQTTLKLSLGLLESAVLGVVLSTIFVIMQGGIANPTLKTVIT
                     LLTLLIFGSLVYTTAWREGNKDPNRVHYGRMKKCMAKGLIAGALASAPVIIDYILLLI
                     TSAAGRENFIFLVLFRIFNLYLLQWVNLLINHPFICAIFLLPIPLFAGLGYVMGYQQK
                     SILIRLIYKKKK"
     gene            5353..6084
                     /locus_tag="VHR42_00700"
     CDS             5353..6084
                     /locus_tag="VHR42_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX3025740.1"
                     /translation="MSHRVKKRAVCHRLTLIVSFGVLLLCVTAAVIRITGTSGRIPAD
                     PVKKPKLVYSIFIDIESNRIYLLKSGKLFRSYQCATGKSETPSPAGSYEIISKAHWGE
                     GFGGYWIGIDCPWGIYGIHGTRRPDTVGYAASHGCFRMYNQDCKEIYGLVSVGTKVVI
                     TAGCYGPFGNGFRDIRPHMYGLDVQAVQKRLKELGYYKGRCNGKYENESFKTAIHMFQ
                     KDNCLKVTDVIYQSMVIKMGFVIME"
     gene            6231..7226
                     /locus_tag="VHR42_00705"
     CDS             6231..7226
                     /locus_tag="VHR42_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX3025741.1"
                     /translation="MRKYSEQESPRIIRDFCVYMETIRGKSPGTVQEYYTDLRTFFRF
                     IKQYRNLCPKDAEFDSISIEDVDLSMIGSVTLHDIYEYLFFITRVRRNNASTRSRKIS
                     SLRSFYKYLCDKTGVLRENPTQNLEMPKKKKSLPKYLTLEESIDFLNGIDGEYRERDF
                     CIMILFLNCGLRLSELVGLNLSDIHDNYLTVTGKGNKERSVYLNDACSQALSSYRSRR
                     PNEGVKDKDALFLSRFKTRISPKTIQWIVKKYLKTAGLSEKGYSTHKLRHTAATLMYQ
                     HGGVDIKALQEILGHENLNTTEIYTHISNSQLEKAAKSSPLANFKIRKASKNQEP"
     gene            complement(7301..8161)
                     /locus_tag="VHR42_00710"
     CDS             complement(7301..8161)
                     /locus_tag="VHR42_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEX3025742.1"
                     /translation="MNFNKPGPGVRKDLPKKRRFIVFFEIYFRKFWKLIQANLLFCAA
                     CFPFFIPLLFLLTSKIEVNYIYLIGFLPIAGIGPAIAGFTYILRNFAREQHAFIWMDF
                     RDTYKSNWKQATLVTLINIIVGTVLYMVISFYYANLHKGQLMVVPFSLSLVIAAIFIF
                     VQYYLYTMLITFDLTTRQLYKNAVIFAVVGLGKNIVTTLICGIIVLLIYAFKPLTLAL
                     IPFIFLATVGLIISFNSWPLIEKHLMPKTEGDEEYGAETDSEESSKNADQGRTVEQDH
                     IFEDSNPDKE"
     gene            complement(8197..9081)
                     /gene="xerD"
                     /locus_tag="VHR42_00715"
     CDS             complement(8197..9081)
                     /gene="xerD"
                     /locus_tag="VHR42_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX3025743.1"
                     /translation="MDENIQGFTEYLLNQKSASKSTVQSYQRDIRQFRQYLEQKKVKS
                     LTAVDSAILSGYNEYLKKCGKSSSTISRFIASIRCFYRYLISIGAAHENPAAGLKLAR
                     EKKHLPEIMTNEEVELLLDQPACIDFKGYRDKAMLEILYATGIRVSELISLSIGDVNL
                     DLGILSCRGEGKDRVIPIYPAAVKAVSQYLYQARGVLIDDSNSGPLFVNLSGNHLTRQ
                     GFWKIIKMYTEQAGIRKRITPHTLRHSFAAHLLENGADLKSIQEMLGHADISSTQIYT
                     KIVKNRYRDVYNKCHPRA"
     gene            complement(9101..9739)
                     /locus_tag="VHR42_00720"
     CDS             complement(9101..9739)
                     /locus_tag="VHR42_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX3025744.1"
                     /translation="MVRTVAGKRSRSAARGLPANPAFNQYLTIAVLIIVGIVFGAILA
                     NQAGIVKKSSFSFFVQDMLSKDVASKGFASLFLSSFFSSAVLICAAFFLGLCAFGQPG
                     NLLIPVFKGAGIGLSMSYVYIQYGIKGMGICLLFLLPQAVLSSLSIIVACREGIRFSN
                     TLAKTIFHGSQQTLWPDFSNLCYKYVFCFVLVLAAAVIEAFSIMGFSRLFFS"
     gene            complement(9836..10642)
                     /gene="proC"
                     /locus_tag="VHR42_00725"
     CDS             complement(9836..10642)
                     /gene="proC"
                     /locus_tag="VHR42_00725"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX3025745.1"
                     /translation="MADRIGFIGAGNMAGAIIEGMIKSSVAIPENIAVYDLNSEKTSA
                     FLSRGIQVKQSICEIASFSNILFLSVKPQNYEEVLSNLKDCFSVEKTILVTIAAGISS
                     DFIKGFLGDGCKVVRAMPNTPLLLGEGATALCRCLPATDEEFDRVKKIFLAGGVVEVL
                     DEDHMNAVIAVNGSSPAYIYLFAKAVIDGAENQGIDRQVATNLIAKTLIGSAKMLTDS
                     GYGPDELIRMVSSPGGTTLKALEALYDHDFEDAILDAMERCTARAEELGK"
     gene            complement(10864..12228)
                     /gene="scfB"
                     /locus_tag="VHR42_00730"
     CDS             complement(10864..12228)
                     /gene="scfB"
                     /locus_tag="VHR42_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HEX3025746.1"
                     /translation="MIHKYRLGGYSIVLDVNSGTVHVLDDVTYDLLDYAENALPGNLP
                     QAAVAALSEKYGRESLEDSWKEIAQLHSQGLLFSPDVYDAYRNAGEHAPIKSMCLNIS
                     HDCNLRCKYCFASTGDFGSGRKLMPFEVGKAAIDFILEKSAGRHNLEIDFFGGEPLMN
                     FDTVKKVVEYGREASRPVHKNIRFTITTNGLLLDDDKIDFINREMSNVVLSIDGRKEV
                     NDKMRIRTDGTGSYDEILKNFKNLVEKRNHENYYVRGTFTRENLDFMADVLHLADLGF
                     DQVSVEPVIAGEELPYSIGEAELPGIFEEYEKLSEEIIKRHRQGRKFNFFHFMIDLDH
                     GPCAIKLLKGCGCGNEYVAVTPDGDIYPCHQFVGHDEFVMGNVLNHDGGMIDEKMKDQ
                     FSKANIFTKAGCSECWAKFFCSGGCNANNHEFNGSIFKPHSLSCKMEKKRLECAIMIK
                     AALS"
     gene            complement(12353..12490)
                     /gene="scfA"
                     /locus_tag="VHR42_00735"
     CDS             complement(12353..12490)
                     /gene="scfA"
                     /locus_tag="VHR42_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HEX3025747.1"
                     /translation="MKHIKTLNTRNLKNTAAKGGCGECQASCQSACKTSCTVANQKCE
                     K"
     gene            complement(12514..12927)
                     /locus_tag="VHR42_00740"
     CDS             complement(12514..12927)
                     /locus_tag="VHR42_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HEX3025748.1"
                     /translation="MKSEQIKQKGNLNGTAMQILMGVLGGFAFSLLILLLLSFVMTVK
                     DLPQNAVDPLACVSIVCGSFFGGWIAARIRRSQGMIYGAVTGFIFFLLLWATGAILHQ
                     AGFGTLLLIKLLLSIGFGCLGGIAGVNVKRKKQRI"
     gene            complement(13047..13388)
                     /gene="yajC"
                     /locus_tag="VHR42_00745"
     CDS             complement(13047..13388)
                     /gene="yajC"
                     /locus_tag="VHR42_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX3025749.1"
                     /translation="MFNLHFLPLAASASTANTSTIGSLAVTFLPLLLIFVVFYFLLIR
                     PQRKKEKDTQKMRSNVQVGDEVITIGGIMGRVVSIKDDTLVIETAADRNKVRIKKWAI
                     QTNTTVHDEIA"
     gene            complement(13550..14680)
                     /gene="tgt"
                     /locus_tag="VHR42_00750"
     CDS             complement(13550..14680)
                     /gene="tgt"
                     /locus_tag="VHR42_00750"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX3025750.1"
                     /translation="MFELIKIQGKARRGRLDTVHGVVETPAFMNVGTQAAIKGGVSSH
                     DLAGLKCQVELCNTYHLHLRPGDEVIRELGGLHKFMGWNRPVLTDSGGFQVFSLSGLR
                     KIKEEGVYFSSHIDGRKIFMGPEESMQIQSNLASTIAMAFDECVENPAEYDYARQSCE
                     RTYRWLCRCKEEMERLNARHDTLNRSQMLFGINQGSTYEDLRIENMVKSSGLDLDGYA
                     IGGLAVGEPASEMYRIIDCVEPYMPENKPRYLMGVGTPTNILEAVSRGVDLFDCVMPS
                     RNARHGHVFTFQGKMNLFNAKYERDSLPIEEGCGCPTCRQYSRAYLRHLFKAGEILAL
                     RLCVMHNLYFYNTLMEKIRESLDNGTFDRLKADYSPTIDRRI"
     gene            complement(14703..15734)
                     /gene="queA"
                     /locus_tag="VHR42_00755"
     CDS             complement(14703..15734)
                     /gene="queA"
                     /locus_tag="VHR42_00755"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX3025751.1"
                     /translation="MKKSDFYYELPQELIAQEPLRQRDCSRLMTLDRETGKTGHYLFH
                     DILDLLREGDCLILNDSRVLPARLYGQKEKTGGHIEFVLLNEKRKDVWEVILRPGRMA
                     KPGMRFLFGDGLLKAEILEVTDEGNRIVKFEYDGNFYEILEKIGEMPLPHYITRKLPE
                     SEKEYYQTVYAKQPGSAAAPTAGLHFTPELLEKLRKKGVGLGYLTLHVGLGTFRPVKA
                     DRIEDHVMHFEHYELPQKTADLINETKKNGGRVIAVGTTSCRTMETVAHETGRIQAAE
                     GWTGIFIYPGYQFKAIDGIITNFHLPESTLIMLISAFAGRDHVLGAYEEAIREKYRFF
                     SFGDAMLIC"
     regulatory      15898..16071
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            16257..17336
                     /gene="asd"
                     /locus_tag="VHR42_00760"
     CDS             16257..17336
                     /gene="asd"
                     /locus_tag="VHR42_00760"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX3025752.1"
                     /translation="MKKYRVGIIGGTGMVGQRFATLLNNHPWFDVTVIAASSRSKGKT
                     YSQAVDGRWAMKEPIPEKLKDLIVYDAVTDVAEVSKNVDFVFCAVDMKKDEIKALEET
                     YAKAECPIISNNSANRFTPDVPMIIPEINPDHAEAILSQRKRLGTKRGFIAVKSNCSL
                     QSYVPALHPLKKYGVTKALACTYQAISGAGKTFDRWPEMIDNVIPFIGGEEEKSEKEP
                     LKIWGSLENGVIVPAAGPSITTQCIRVPVADGHLAAVFASFEKKPQIEEVISDWENYE
                     GRPQELGLPSAPKQFIHYFTEDNRPQTRLDRSLEGGMAVSVGRLRRDSQYDIKFVSLS
                     HNTIRGAAGGGILMAELLCAEGYLD"
     gene            17470..18357
                     /gene="dapA"
                     /locus_tag="VHR42_00765"
     CDS             17470..18357
                     /gene="dapA"
                     /locus_tag="VHR42_00765"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX3025753.1"
                     /translation="MKKQLFTGSGVAIVTPMHPDGSVDFDLLGKLIEFQIKSGTEAII
                     ICGTTGESPTLSHDEHISCVKFAVEKTAGRIPVIAGAGGNNTKSSAELSIEVEQTGAD
                     GLLLVTPYYNKTTQTGLIRHFFYIADRVNVPIILYNIPSRTGLDIKPETYLELSKHPN
                     ITGIKEANCNIESVAKTASLCGDEMAIYSGNDAETLPILSLGGKGVISVLANIMPKEM
                     HDLCKKFFDGDVAGSRKLQLDLMGLISALFIEVNPIPIKHAMNLIGYAVGECRMPLAP
                     MLEKNAEILKTELKRNHLL"
     gene            18479..>18855
                     /locus_tag="VHR42_00770"
     CDS             18479..>18855
                     /locus_tag="VHR42_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX3025754.1"
                     /translation="MNRIILSGCNGKMGGVVSKTIMENRDCVIVAGIDVKSGQQEDYP
                     VFSDPSGFQGKADVIIDFSNPGMLDCLLTFAKNRKIPIVIATTGFSRAQIQEIEAAAK
                     EIPVFFSANMSLGVNLMIEIAKKA"
BASE COUNT         4968 a         4541 c         4386 g         4960 t
ORIGIN      
        1 tctggatgca agaaaggaaa tccctgttcc ggcggctatt cccaacaaac agccgatgag
       61 tgaaatcagg agcgcttcaa acagaaactc cattagaacg acgctttttt tcgctccaat
      121 tgcctttttt attccgattt cccttgtgcg ttcgttcacc gaaacggtca ttactgtcat
      181 aattcccaga cctgcaacta cgagtgagat tgcgccgaca gcggaaatta tcaatgtcac
      241 aatggacaga agattggaaa gcttctcttt ctgtttaaaa atattatccg ccacataatt
      301 gtcacttact ccgtgaatct ggttaagctt actgatgatt ttgttcccga tcgaatctgt
      361 atttttgtcc gaggttgttt taatggcaat ttgatcgtat cccacctggc cttttagatc
      421 tttggcggtc gagacaggca tatagacaaa agacggaata taatttccgg ccagactgtt
      481 cagcagactg cttcccgttt tgacgatgcc gataattgtc aattcccggc tctccgtacc
      541 tacaaaaacc gttattttct ttccgctgat gttatctctt ttataagccg accgggcaaa
      601 actgtcatca accaaacaga cattcgcgcc ggttttaaca tcgctctgcg aaaacattct
      661 gccgtgaata attttcaggg agataatttt ttccgcatcc gacgaaacgc cccaaatcaa
      721 agagtccttc tgactgcctc tcaaaaccga gacagcggat tgcatcacaa caggcattgc
      781 ggactgaacc cctttgacgt tcaggcaggc cgtcacgtct ttgtcattaa tggatgcaga
      841 ataggctccg gtttgcgtgg tctttgatct gacatccagt ccgttgatac caagctgatc
      901 cagttgaccg gtaatggcca attttcctcc ctccccgatt gctccgatga cgatgaccga
      961 ggccactccg atcataatgc cggaaacggt caggatggtt cgaatttttt tcctgccgag
     1021 atttctcatc gcacaaatta aatattctat cattttgaag aaacccccga cccggttttt
     1081 gcagttttta accggacgat ctctccgctt ttgagagtgg acggaggatt tagtaaaatt
     1141 tcatcaccct gactgagacc cttgaccacc tgcacgcagt cgtcgtattc gtcgccggtg
     1201 acgacattct gacgcattgc ccggccgttc cgataacgga agacatattc gacattgctg
     1261 tcatcctgca gaacagcttc ataaggcacg gcgatagttt tagggttgtc ggaagttaca
     1321 atttcaacat ttgcagtcag attcggcttg agaccggata ccggtttttc aatcttgacg
     1381 actacggcta ccgtgctgcc cgtaccgctg agagaactgc tctggctggc ggaagggtat
     1441 attcttgcaa catatccttt gcagcacccg ctgaaaccgt ctccggtgac ataggctgtc
     1501 tgcccgtctt tcactttagt aatcaggctc tcctcaacct gcgcggttac ttcgagatgg
     1561 ctgaggtcgg aaatggtaat gattgctgcc ctcgggttgg taaaagcgcc gcaaaccgcg
     1621 gatatttggg ttaccgttcc tgaaatcggc gcgcacactt ctgacggaat ctgcgagacg
     1681 gatgccgaag aggagcttcc gctttgcgat gaagcactgc cggaactgct gtattgcgtc
     1741 aggttatatt gttttgccaa actgtttaag tattcgctct gctggctgtc ggcggatgat
     1801 gaggcagaaa cggtggagga gccgctttgc gaggataccg atccgctttg aatcccctgc
     1861 cgggtagcct gttggtcaat gtccatcagc ttttgcgatt ttttgacgct gtcgccgact
     1921 gcgacgctga ttccgctgat tttcatcgag taaccataac ttacggctga tgtatattcg
     1981 gagctgatgg ttccgctgca ggaaatggtg gatttcatgt tgccgttcat gattcgtacg
     2041 gcggtaacag tggtaattga ggaccccgcc aactggggaa tcagtatcag aagaagagcc
     2101 gccgcaaggg taacgcctaa tagaattaca tattttttca tcccgatcac tcccgtactc
     2161 ttattgtgtc aacagccggg ctatttatga tacgggaccg taaaatttaa aaggttctgt
     2221 tcagaatgtc gatgcttcac tttcgggcta aagaataaat gaagagattc cgaaaaagat
     2281 atagtaaaaa taaaatgtaa tggagtcgga tctatgagtt tgatacattg cacgagtgac
     2341 tgcaaatttc agaatgaagg atattgtcag ctggatgaaa ccgcacgggt cacaaatttg
     2401 tcggaatcaa atggctgtca ttattttgtt cccaaaacag aaatccgcag aaccgggcgc
     2461 aaaacgaagg gagattccat cagataacgg cttcaaaggc ttccctgaca ctttttacac
     2521 cgatcaactc gatctcatac ttttccctgc cctgcagggt attcaggtta tgacggggca
     2581 gaatacagcg tttgaagccc agtctggacg cttcagcgat tctggcctcc gccctgcccg
     2641 cagcgcgtat ttctccggcg agcccgagtt ccccgaatgc cacggtatag gggtctacgg
     2701 gctgatcctt taggctggat accatggata aggcgactgc caaatccgcc gccggttcat
     2761 ccagcctcag tcctccgacg atatttaaat aggcgtcggc gttggaaaaa tagtaacccg
     2821 cgcgtttttc cagtaccgcc agaagaaccg acattcggtt atagtcaaaa ccggttgaca
     2881 tccgccgggg atttccgtag ccggatgacg tgacgagagc ctgcacttcc gcgagaatcg
     2941 gccttgtgcc ttccatgacg caggcaacgc ttgtcccgga aatattgagg ggtctgcccg
     3001 aaagcagcat catcgaggga ttttccactt cccgcaagcc ggtgtcggtc atttcgaaga
     3061 cgccgatttc attggtggag ccgtaacggt ttttaaccgc acggagaatt cggtaggaca
     3121 ggttcctttc gccttcaaag taaagaaccg cgtcaacgat atgttccagc accttcggcc
     3181 cggcaatcgc gccttccttg ttaacatggc cgaccacaaa caacggaatg gaagtgaatt
     3241 tagccgcgtt gagcagctcg tgggtgcatt ctcttacctg gctgacgctt cccgccgagg
     3301 aagacatatc ccgcttatac atagtctgta ttgagtcgac aatgacgatg tccggttttt
     3361 ccccataaat gacctgaaca acattttcca tatcggtttc agcgagcaaa aaaaggttcg
     3421 ggctgtcgat attgagcctc tgcgccctga gtttaatctg tcgaaccgat tcctctcccg
     3481 tgatatagag aatcttttta tcctgcccca aaaagccaca gatctggagc agaatcgttg
     3541 attttccgat accggggtcc ccactcagaa ggatcagcga accgcggaca atgcctccgc
     3601 caagcacccg gtcgagttct ttaagccccg ttgagtaacg ttcctcattt tccattgaca
     3661 gctcattgat ctttaccgaa cggcttacgc cggacgcggt cggatgtgat tttgccgacg
     3721 gttttaccgg ttccctgatt tcctctacca gggtattcca ctccccgcat ccggggcatt
     3781 ttccatacca ttttggcgat tcataaccgc actgggtgca gacatatata ctttttgact
     3841 tcattgccat tgaacacacc gtccccaatc aatttatgtt ccggagccgt agctcaatac
     3901 ggcgctgaat atagaaaaag caattttttg ctgatagctg ttattttcaa gatttttgca
     3961 ttctgtcgga ttagataaaa agccgcattc caccatgact gacggagatt tggcgtaata
     4021 aagcagaaac aaattttttt cggccttctt gatcatccga ttgttcgtcg gctgaagctg
     4081 ctcgcggatt tgtttttgga tgatttccgc caaagttttg ctctgtggat tattttggga
     4141 gtaaaatacc tgggccccgc tgtatcggct gctttcataa atattctggt gaatgcttaa
     4201 aaacagcgcg tcggggtaag aactggccag cttaagccga ttatgaagat cggaagtctt
     4261 tttttggcgg atcgtattgc ttccgctgtc ataaatggct ttgtcactat ctctcgtcat
     4321 aatgacctga tatccggcca accgaaaaaa cgttttgagt ttcagagaaa ttgagagatt
     4381 aatgtctttt tcaatcactc cgtcataacc gatcgcgccg ccgtcttctc ccccatgccc
     4441 ggcatctata ataattgttt tttcttcggt cttcacgtct tcagaaccga aagcccgtga
     4501 cgacctgaag gccgttaaat cgacaaaaat aaccaaaaga atcacaaaag aaacaatggt
     4561 gacctgaatc aataagtctc cggaaatgtt ttgcctgcgc ataagataaa ccacccccga
     4621 aaaacactat gccgggagtg ggtaaagtat aacaggcagg ctgtaacaat tattttttct
     4681 ttttataaat cagtcgaatg aggatggatt tctgctggta gcccataaca tagccaagcc
     4741 ctgcaaacag aggaatcggc aaaagaaata tggcgcagat aaagggatgg ttaatcaaaa
     4801 ggttaaccca ttgaagcagg tacaagttaa atatgcggaa aagcactaaa aagataaaat
     4861 tttcccgccc tgccgcggaa gtaatcagaa gcaggatgta atcaatgatg acgggagccg
     4921 aagcaagagc gcctgcaatc aggccttttg ccatgcattt tttcattcgg ccatagtgga
     4981 cacgatttgg gtctttattg ccctcccgcc aggccgttgt ataaaccaga ctgccaaaga
     5041 tcaaaagagt cagcagcgta atcacagttt tcagcgtcgg atttgcgatt ccgccctgca
     5101 tgattacaaa aatggtagac agaacgaccc caagaacagc cgattccaaa aggccaagtg
     5161 acaattttaa tgttgtttgt ctcagcacag tgtttcctcc cgagttactt ttaaaaacat
     5221 tatataacaa tcaccggaaa taaacaacaa cccttccacg gctctggtgg taaacaccgc
     5281 gttccgggca tatggttata ttggttcaga tgaaaatctg ccgtcttcca tagactaact
     5341 ccggaggcat gtatgagtca cagagtgaaa aaaagagccg tttgccaccg attaaccctg
     5401 attgtttcat tcggggtttt attactttgc gtcacagccg ctgtaattcg tatcaccgga
     5461 acttccggac ggattcccgc cgacccggtc aaaaaaccta aattggttta ttccatattc
     5521 attgatattg aatccaacag gatctatctt ctgaaaagcg gaaaactgtt tcgttcctat
     5581 cagtgcgcca ccggaaaatc cgaaacccct tcccctgccg gcagttacga aatcatctct
     5641 aaggcacact ggggggaagg cttcggcgga tactggatag gaatcgactg tccatgggga
     5701 atttacggca tacacggaac cagaaggccg gacacagtgg gttacgccgc gtcccacggc
     5761 tgttttcgga tgtataatca agactgcaaa gaaatttacg gtctggtatc tgtcggaact
     5821 aaggtggtca ttacagccgg atgttacggc ccgtttggga atggctttcg ggatatccgg
     5881 ccgcatatgt acggacttga tgtgcaggct gtccagaaaa gactgaagga actgggctat
     5941 tataaaggac gatgcaacgg caagtatgaa aatgaatcgt ttaaaaccgc catccatatg
     6001 tttcaaaaag ataactgcct gaaggtaacc gacgtgattt accaaagcat ggtgataaaa
     6061 atgggttttg tgattatgga ataaaagttg tcattatccg ggaaaagcct tataataaaa
     6121 ttgcagtctg agacgacagc cgggtttccg acaggcgtcg cggccctgcg cgggaggact
     6181 gtcatccttg taagtcttcc tatattttgt cgattgggat gaggcttttc atgcggaaat
     6241 attcggaaca ggaatctccg cggatcatcc gcgatttttg cgtttatatg gaaaccatcc
     6301 gaggcaaatc gcccggaacg gtgcaggaat actacaccga tctgcggaca tttttccgtt
     6361 ttatcaagca atacaggaat ctttgcccga aagacgccga gtttgattcg atttcaatcg
     6421 aagatgttga cctttcgatg atcggctcgg taacccttca tgatatctat gaatatttgt
     6481 ttttcatcac ccgtgttcgc cgaaacaatg cttcgacccg gtcgcgcaaa atctcgagtt
     6541 taaggtcttt ttataaatat ctctgtgaca aaacaggagt tttgcgtgag aatcccacgc
     6601 aaaaccttga aatgccgaaa aagaaaaagt cactccctaa atatctgaca ctcgaagaaa
     6661 gtattgactt tctcaatggt attgacggcg aataccgtga gcgggatttc tgcattatga
     6721 ttctgttttt gaactgcggg ctccgtctct cggaattggt cggcctcaat ctttccgata
     6781 ttcatgacaa ctatctgacc gttacgggaa aaggaaacaa ggagcggtcg gtttatctca
     6841 atgacgcctg ttcccaggcg ctttcttcct atcgtagcag acgtcccaac gaaggcgtaa
     6901 aggataaaga cgctctgttt cttagcagat ttaaaacgag aatcagccct aaaactatcc
     6961 agtggatcgt taaaaagtat cttaaaaccg caggactttc cgaaaagggc tattccacgc
     7021 ataaattaag acataccgcg gcgaccctga tgtatcagca cggcggagtt gatatcaagg
     7081 cgctgcagga aattctcggg catgaaaatt taaacacaac cgaaatctac acccatattt
     7141 caaacagcca gctggagaaa gcggcaaaaa gttcaccgct ggcgaatttt aaaatccgaa
     7201 aagcctcgaa gaatcaggaa ccgtaatcat tgctccggcg aaaaagattc tcccgttaaa
     7261 caaaaaccac cggctcactt agtgagccgg tggtttttgt ttactcttta tccggattac
     7321 tgtcttcaaa aatatggtct tgttcaacgg tacggccttg atcagcattt ttgcttgatt
     7381 cttccgaatc cgtctctgcg ccgtattcct cgtcgccttc tgtttttggc atcaggtgct
     7441 tttcaatcag cggccatgaa ttaaagctga taatcaaacc gaccgtagca aggaagataa
     7501 atggaattaa agccagcgtc agcggcttga aagcataaat aagcagaaca attattccgc
     7561 atataagagt ggtcacaata ttttttccaa gcccgacaac ggcgaaaata acggcattct
     7621 tataaagctg cctggtggtg aggtcaaacg tgatcagcat ggtgtaaaga taatactgaa
     7681 caaaaatgaa aatggcggcg atcacaaggc tcagagaaaa cggcacaacc atcagctggc
     7741 ctttatgaag gttcgcgtag taaaagctga ttaccatata aagtaccgtg ccaacgatga
     7801 tattaatcag cgttacgagg gttgcctgtt tccagttgct tttataagtg tcgcggaaat
     7861 ccatccagat aaacgcgtgc tgttcgcggg caaaatttct taaaatatag gtgaaacccg
     7921 caatcgcggg gccgataccc gcaatcggga gaaaaccaat caggtaaata taattgacct
     7981 caattttaga tgtcagcagg aagagcagag gaatgaaaaa aggaaaacag gcggcgcaaa
     8041 aaagcagatt tgcctgaata agcttccaga actttctgaa ataaatttcg aaaaatacaa
     8101 tgaagcggcg ttttttaggt aaatcctttc tcactcctgg gccgggtttg ttgaaattca
     8161 taaaaaatcc tgccatattt gttaatactc ctttgcttac gctcttggat gacatttatt
     8221 atagacatcc cgataccggt tttttacgat ttttgtataa atttgagttg aagaaatatc
     8281 cgcgtgcccc agcatttcct gaattgattt aaggtctgcc ccgttttcca acagatgggc
     8341 ggcaaaggaa tgccgcagag tatgcggtgt aatccgtttg cggatgcctg cctgctcggt
     8401 atacatttta atgattttcc agaagccctg tctggtgaga tgattgccgc tgagatttac
     8461 aaacagcgga ccgctgttgc tgtcgtcgat cagaactccg cgcgcctggt ataaatactg
     8521 ggaaaccgcc ttgactgccg ccggataaat tggtattacc cggtccttcc cttcgcccct
     8581 gcaggaaaga attccaagat caaggttaac atcaccgata ctcagtgaaa taagctccga
     8641 aacccttatg ccggtagcgt aaagaatctc aagcatggct ttatcccgat aacccttaaa
     8701 gtcgatacag gcgggctgat caagcagcag ctctacttcc tcattggtca tgatttccgg
     8761 aagatgtttt ttttctcttg caagcttaag accggcggcc ggattttcat gtgcggcccc
     8821 aatcgaaatc agatatcggt aaaaacaacg gattgatgcg atgaaacggg aaattgtgga
     8881 cgatgacttt ccacacttct tcagatactc attgtatccg gatagtatgg cgctgtcaac
     8941 tgccgtaagg ctttttactt ttttctgctc cagatactgc cggaactggc ggatatcccg
     9001 ctgataagac tggacagtgc tcttggatgc agatttttga ttcagaagat attctgtaaa
     9061 tccctgaata ttttcgtcca taattcattt cccttccgtg ctaagaaaaa aataaccttg
     9121 aaaaacccat aatcgaaaag gcctcgatca ctgcggctgc taacaccagc acaaaacaaa
     9181 aaacgtattt ataacaaaga ttggaaaaat cgggccaaag tgtctgctgg cttccatgaa
     9241 atatcgtttt cgccaacgta ttcgaaaacc ggatgccttc tctgcaggca acaataatgg
     9301 agagtgatga aagaacagcc tgtggaagaa gaaacagcaa acagatcccc atgcccttga
     9361 ttccatattg aatgtaaaca taactcatgg aaagcccgat tccggcgccc ttaaaaacgg
     9421 ggatcaaaag attgcccggc tgaccaaaag cgcaaaggcc gagaaaaaac gcagcgcaaa
     9481 tcaaaacggc tgatgagaag aaagaggaca gaaacaggct ggcaaagccc tttgaagcca
     9541 catcttttga aagcatatcc tgaacaaaaa agctaaagct gcttttttta acgatacccg
     9601 cctgattggc gagaattgcg ccaaaaacaa taccgacaat gatcagcacc gcgattgtca
     9661 ggtattgatt aaacgcggga tttgcgggca atccccgcgc ggcggaacgg cttcttttac
     9721 cggcgacagt tcgaaccaag ggcttggccc cctttgcaag tttgttttaa taagacttat
     9781 gcaagagggg gctttattat tcccgtttcc cgaaggcagc gggaaatatt ccgatttatt
     9841 tgccgagctc ttccgcacgg gcggtgcatc tttccattgc atcaaggatg gcgtcttcaa
     9901 aatcgtgatc atagagcgct tcaagcgcct tgagggtcgt accccccggc gaggatacca
     9961 tccggatcag ttcgtccggg ccgtaaccag aatcagtcag catctttgct gaaccgatca
    10021 gcgtcttcgc aataaggttt gttgcaacct gacggtctat tccctgattc tccgccccgt
    10081 cgatcacagc tttggcaaaa agataaatat aagcggggct gctcccgtta acggcaataa
    10141 cggcgttcat atggtcttca tcaagcactt cgactacgcc gccggccaaa aaaatctttt
    10201 taacgcggtc aaattcttcg tctgttgcgg gcaggcaacg gcaaagggct gtcgccccct
    10261 cgccaagcaa aagcggcgta ttgggcattg cgcggacaac cttgcagcca tcccctaaaa
    10321 agcctttgat aaagtcggaa gaaatccccg cggctatcgt gacaagaatc gttttttcta
    10381 ctgaaaaaca atctttcaga ttgcttagta cttcttcata attctgcggc ttgaccgaca
    10441 aaaagagaat attgctgaaa gaggcaattt cacaaataga ctgtttgacc tgaattcccc
    10501 tgcttaaaaa agccgacgtc ttctcgctgt ttaagtcata taccgcaatg ttttccggta
    10561 ttgcgacgct gctttttatc attccttcaa taattgcgcc tgccatattg ccggcgccga
    10621 tgaaaccgat ccgatctgcc attttctcgc cttcctacct aacatgtaaa ataaatataa
    10681 accaattttt cagcgcaatc aataataagt tttgatttta cttaatttta ggttacttgc
    10741 acaaagttta tgtcaaaaca attatgtaaa cgtacaaata ttatgttaac tacgttatgt
    10801 aaacctatgg tcaaacgaaa cgggcggcgc tttccgtccc cttgtttcgt cgggttttat
    10861 cgctcaggaa agagccgcct tgatcataat ggcgcattca agccttttct tttccatttt
    10921 gcaggaaagg ctatggggct tgaaaatact tccgttgaat tcatggttgt tggcattgca
    10981 tccgccgctg cagaaaaact tcgcccagca ttcggagcag ccggccttgg taaaaatgtt
    11041 ggccttggaa aattgatctt tcattttttc gtcaatcatc ccgccgtcgt gattcaaaac
    11101 gttgcccatg acaaattcat cgtggccgac aaactggtgg cacggataaa tatccccgtc
    11161 cggggtaacg gcaacatact cgttgccgca gccgcagcct ttgagcaatt tgatcgcgca
    11221 agggccgtga tcaagatcaa tcatgaaatg gaaaaagtta aattttctgc cctgcctgtg
    11281 gcgtttgatg atttcttccg agagtttctc atactcttca aaaataccgg gcagttcggc
    11341 ttcgcctatg gaataaggaa gttcttctcc ggcaataacg ggctcgacgg aaacctgatc
    11401 gaagcccaga tcggccaaat gaagaacatc ggccataaaa tcaaggtttt ctctggtaaa
    11461 cgttcctcgg acataataat tttcatgatt ccgtttttca acaagatttt tgaaattttt
    11521 aaggatttcg tcgtaactgc ctgtcccatc cgtacggatc cgcatcttat cgttgacttc
    11581 tttgcggccg tctatcgaca gcacgacgtt gctcatttcc ctattgataa aatcaatttt
    11641 gtcgtcgtcc agcaaaagcc cattggtagt gatggtaaaa cgaatgtttt tatgaacggg
    11701 ccgcgaggct tcccggccgt attcgaccac tttctttacc gtgtcaaaat tcatcagcgg
    11761 ctcgccgccg aagaagtcta tttcgagatt gtgacggccg gccgattttt ccaaaataaa
    11821 atcaatcgcc gcttttccca cctcaaaggg catcagtttt cttccgcttc cgaaatcacc
    11881 ggtggaggcg aagcaatatt tgcagcggag attgcagtca tgggatatgt tcagacacat
    11941 ggatttaatc ggcgcgtgtt cgccggcgtt acggtaagcg tcgtaaacgt cgggggaaaa
    12001 cagcagcccc tgactgtgca gctgggcaat ttccttccag gaatcttcaa ggctttcccg
    12061 gccatatttt tcagacagcg ccgcaacagc agcctggggc agattaccgg gcaatgcatt
    12121 ttccgcgtag tcgagaagat cataggttac gtcgtcaaga acatgaacag ttccgctgtt
    12181 gacatcaagc acgatcgaat agccgccgag cctgtattta tgtatcattg attttttacc
    12241 tccgtgcgcc atgcgctttc aataaatcca gtctgccgcc ggtaaaccgg caaaaattca
    12301 gggacgtaaa acgtccctgt agattggagt gttttgtttt agtcctcagt cgttatttct
    12361 cacatttctg gttggcaacc gtgcaggaag ttttgcaggc tgactggcag gaagcctggc
    12421 attctccgca tccgcctttt gcagcggtat tcttcaggtt tctggtgttt aatgttttga
    12481 tgtgtttcat gatgtatatc tccttagaag tttttatatt cgttgttttt tacgtttaac
    12541 attgactccc gcaattccgc cgaggcatcc gaaaccgatt gacagaagaa gcttgatcaa
    12601 aagcagcgta ccgaacccgg cctgatgcag gattgctccg gtcgcccaga gaagcagaaa
    12661 aaagatgaac ccggtgacag cgccgtaaat cattccctgt gaacgccgga ttctagccgc
    12721 gatccaacca ccgaagaacg aaccgcagac gattgataca caggcaagcg gatcgaccgc
    12781 gttttgcgga agatctttta ccgtcatgac gaacgatagc agcagcagaa tcaggagact
    12841 gaaggcaaaa ccgcccagca cgcccatcag tatctgcatc gccgtgccgt taaggtttcc
    12901 cttttgtttt atctgttcgg atttcatggc aatcccctct taacgcatat tattcggtgt
    12961 attttactcg gctgtcagcc ttatataata caatttattc gggaaccgcc tggtttatgc
    13021 agaccgaaac ggacaaatag gggaaactac gctatttcat cgtgaacggt cgtgttggtc
    13081 tggatcgccc attttttaat acgcaccttg ttacggtccg ccgcagtctc aattaccagc
    13141 gtgtcatcct taatggagac cacacggccc atgattccgc cgatcgtgat gacctcgtcg
    13201 ccgacctgaa cattagagcg cattttctgt gtgtcttttt cttttttacg ctgcggcctg
    13261 atcagaagaa aataaaacac cacaaaaatg agaaggagag gaaggaatgt caccgccagt
    13321 gaaccaattg tgcttgtgtt cgccgttgaa gcagatgctg ccagcggcag aaaatgaaga
    13381 ttaaacaact acttacacct ccaagtttat ttgacctatt ttaatcttgt cccattctgt
    13441 taaaacaatt tgaagaggaa accgtctaaa gttttcgcag tgtgggcttc aagcgaaaaa
    13501 ctgcatttta attatacctg caatatcata acttgacaag atgattttgt caaatccttc
    13561 tgtcgattgt cggggaataa tccgccttca ggcggtcaaa ggttccgttg tccagagact
    13621 cccggatttt ctccataagc gtattgtaga aatagaggtt atgcatgaca caaagacgaa
    13681 gcgcgagaat ttctccggct ttgaacaaat gcctcagata agcgcgggaa tactgccggc
    13741 aggtcgggca gccgcatcct tcttcaatcg gaagagaatc ccgttcatat ttggcgttga
    13801 aaaggttcat ttttccctga aaggtaaaga catggccgtg gcgtgcatta cggctcggca
    13861 taacacagtc gaacagatcg accccgcgtg acaccgcctc gagaatgttc gttggcgtcc
    13921 ccacccccat cagatagcgg ggcttgtttt ccggcatgta aggctcgacg cagtcgatga
    13981 ttcgatacat ctcagacgcc ggttccccga cggccagacc tccaatggca taaccgtcaa
    14041 gatcaaggcc tgaggactta accatatttt cgatccggag atcctcatag gtggaaccct
    14101 ggttgatgcc aaacagcatt tggctgcggt tgagcgtatc atgccgggcg ttaagccgtt
    14161 ccatttcctc tttgcagcgg cagagccagc ggtaggttct ctcgcaggac tgtcttgcat
    14221 aatcgtattc ggcagggttt tcgacgcatt catcaaaagc catggcgatc gtggacgcaa
    14281 ggttggattg aatctgcatg ctttcttccg ggcccataaa tatttttctg ccgtcaatgt
    14341 gggaagaaaa gtagacgcct tcttctttga ttttacgaag cccggaaaga gagaatacct
    14401 ggaatccgcc gctgtcggtc aggacgggcc tgttccaccc cataaacttg tgaagaccgc
    14461 caagttcccg aatgacttca tcgccgggac gcaggtgaag atgataagta ttgcagagtt
    14521 ctacctgaca tttcaggccg gcgaggtcat gggaggatac cccgccttta atggcagcct
    14581 gggtgccgac gttcataaac gcgggcgttt ccacaactcc gtgaaccgta tcgagccttc
    14641 cccttctcgc tttaccctgt attttgatca attcaaacat attttcgctc cgtttccgtc
    14701 aactaacaga ttaacatggc atcgccaaag ctgaagaacc ggtatttttc ccggattgcc
    14761 tcttcatagg cgccgagcac atgatctcgg ccggcaaacg cggatatgag catgatcagc
    14821 gtgctttccg gcagatgaaa attggtgatg atgccgtcga tagccttaaa ctgatagccg
    14881 gggtaaatga agattcctgt ccacccctcg gcagcctgta tgcggcctgt ttcatgggcc
    14941 accgtttcca tcgtccggca gctggtagtt cccacggcga tcacccttcc gccgtttttc
    15001 tttgtttcat taatcaaatc ggccgttttc tgcggcagct cataatgctc aaaatgcatc
    15061 acatgatcct caatccggtc ggctttgacc gggcggaagg ttcccagccc cacatgtaag
    15121 gtcagataac caaggccgac gccctttttt ctgagttttt cgaggagctc gggtgtaaaa
    15181 tgcaggcctg ccgtcggcgc cgcagccgat ccgggttgtt tggcataaac ggtctggtaa
    15241 tattccttct ccgattccgg aagttttcgg gtaatataat gaggaagggg catctcccct
    15301 attttttcca gaatttcata gaaattcccg tcgtattcaa attttacgat tcggtttcct
    15361 tcatcggtta cttccagaat ctccgctttg agcaggccat cgccaaacag aaagcgcatg
    15421 cccggttttg ccatccggcc gggtctgaga atgacttccc agacatcttt tcgtttttcg
    15481 ttgagcagga caaattcaat atgcccgccc gtcttttcct tttgtccgta aagccttgcc
    15541 ggcagcaccc gggaatcgtt cagaatcagg cagtcccctt cccgcagaag atcgagaata
    15601 tcatggaaca gataatggcc ggtttttccg gtttcacggt caagcgtcat aagacgggaa
    15661 cagtcccttt gcctgagggg ctcctgcgcg atcagttcct ggggtaattc gtaataaaaa
    15721 tccgattttt tcatttgatc aatctgacct tccaaatcgt taaattttaa aaatcctgaa
    15781 acagccgaaa aaactgctgc tttttccggc ggatttatca gaaaactact tgatttcagc
    15841 tgctgaaagg gtttgacatg cagcgtttta gatggtaaaa taaatctaaa ttgaatatga
    15901 tgtgatagag gtgcgttttt aaagagtagt ttaccggagg ccgccggggc cgttgaacgt
    15961 aattgaaagg taaaaacgcc gaagggataa gcttcggctg cttatcgcct ggttgcccgg
    16021 cgaataactg tgtgactgtc atcatgtaat tgatggagag ctatccggca ctttttaaca
    16081 aagcgtcaat gcttctatta tattgcatga atgaccggtt ttcaaccggt ttttttattg
    16141 catagctgtt aattccggtc ggcccgtggg atgcgggcga gtcatgaaaa cagaaatatt
    16201 caacaaaaat gcttgtatat gtgtcgcaaa gggccatatg gcaggaggtt aaagaaatga
    16261 aaaaatacag agttggaata atcggcggaa ccggaatggt cggtcagcgt tttgctacgc
    16321 tcctgaacaa ccatccctgg tttgacgtaa cggttatcgc ggcctcttcc cgctcaaagg
    16381 gcaagactta cagccaggcg gtcgatggcc gctgggcgat gaaagagccg attccggaaa
    16441 aactcaaaga cctgattgtt tacgatgccg taaccgacgt cgcggaagtc agtaaaaatg
    16501 tcgattttgt tttttgcgcg gtggatatga aaaaagatga gattaaggct ctggaagaaa
    16561 cttatgccaa agcggaatgt cccattattt ccaacaacag cgcgaaccgc tttacccccg
    16621 atgtccccat gattattccc gaaatcaatc cggatcacgc agaggcgatc ctttcacagc
    16681 gcaaacggct tggcacaaag cgcggcttta ttgccgtgaa gtcaaattgc tctcttcaaa
    16741 gttatgttcc cgcgctgcat ccgcttaaaa aatacggtgt caccaaagcg cttgcctgca
    16801 cttatcaagc gatctccggc gcaggaaaga cctttgacag atggcctgaa atgatagaca
    16861 acgtgattcc gtttatcggc ggagaagaag aaaaatcgga aaaggaaccg ctgaaaatct
    16921 ggggaagcct tgaaaatggt gtgattgttc ccgcagcggg cccatccatt accacccagt
    16981 gtattcgtgt tccggtagcg gacggccatc ttgcagcggt cttcgcctcg tttgaaaaga
    17041 aaccgcagat tgaggaagtt atcagtgact gggaaaatta tgaaggacgt ccccaggagc
    17101 tcggcctccc cagcgctccg aaacagttta ttcactattt caccgaggat aacagaccgc
    17161 agaccaggct tgaccgttcc cttgagggcg gaatggccgt atcggtcggc cgtctgcgcc
    17221 gcgacagcca gtatgacatt aaatttgtga gcctttctca taatacgatc cggggcgcgg
    17281 ccggcggcgg aatcctgatg gctgagctgc tctgcgcgga aggctatctg gattaatcgg
    17341 cgggtctgat ctttcgcaac attcagagtc ttgctgtttt atatcaggcg agcaaccgcg
    17401 tgtgaatgta tgcgcggaag gatgtttcaa ttatttttat gcgttaacgc gcatggaggc
    17461 tatcagaata tgaagaaaca gctttttacc ggatcaggcg tcgcgatagt aacgcccatg
    17521 catccggatg gttccgtcga tttcgatctg ttgggaaaat tgatcgaatt tcagatcaag
    17581 tcggggacgg aagccataat tatatgcgga acaaccggcg aatcccctac gctttctcat
    17641 gacgaacaca tttcctgcgt caaatttgcg gtggaaaaaa ctgcgggaag aattcccgtc
    17701 atcgcgggag ccggcggcaa caacaccaaa tcgtccgccg aactttccat agaagtggaa
    17761 cagaccggag ccgatgggct cttgctggtc acgccttatt acaacaaaac gacccagacc
    17821 gggctgatcc gtcatttctt ttacattgcg gaccgagtca acgtgcctat tattctgtat
    17881 aatatcccca gcagaacagg gctcgacata aaaccggaaa cctatctgga gctttccaaa
    17941 catccgaata tcaccggtat caaagaggcc aactgcaaca ttgaatcggt ggcgaaaaca
    18001 gcgtcgctct gcggcgatga aatggctatc tattcgggaa acgacgccga gacactgccc
    18061 attctttctt tgggcggcaa gggtgtcata tcggttttag ccaacatcat gcccaaagaa
    18121 atgcatgatc tctgcaagaa attttttgac ggcgatgtgg cgggctcccg gaaactccag
    18181 ctggatctga tgggtttaat cagcgcgctg ttcatcgagg tcaatcccat accgatcaaa
    18241 cacgcaatga acttgattgg atatgcggtc ggtgagtgcc gtatgcctct tgctccgatg
    18301 cttgagaaaa atgcggaaat tctaaaaact gaattaaaac gaaatcattt gctataatct
    18361 gattataggg gaattttcgg caatgcaata attttgtttt ccccctatgc acggggcgga
    18421 ccggattcgt ccccgtgcgg tttttcaaag aatacgctcc gggcgtatgg aggttaaaat
    18481 gaatcgcatt attttaagcg gatgcaacgg aaaaatgggc ggcgtggttt caaaaaccat
    18541 catggaaaac cgggactgtg ttattgtcgc gggaatcgac gtaaagtccg gccagcagga
    18601 ggactaccct gttttctccg atccttccgg gtttcaggga aaagcggacg tcatcattga
    18661 tttttccaat cctggtatgc tggattgtct tctgactttt gcaaaaaacc ggaaaatccc
    18721 tatcgtgatt gccacgaccg gattcagccg cgcgcagatc caggaaattg aggcggctgc
    18781 aaaagaaata cccgtctttt tctctgccaa tatgtcactt ggcgtcaacc tgatgataga
    18841 aattgcaaaa aaagc
//
LOCUS       DATALY010000022         9991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_91461,
            whole genome shotgun sequence.
ACCESSION   DATALY010000022 DATALY010000000
VERSION     DATALY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91461"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1919
                     /locus_tag="VHR42_00775"
     CDS             <1..1919
                     /locus_tag="VHR42_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3025755.1"
                     /translation="TIDGISIAKEIGLGGRINTILQAAFFKLANIIPVEDAVKFMKDA
                     ATASYGKKGEKIVAMNHAAIERGIAGAVKIDVPADWANATDEDACNNVEGDRQELVSF
                     VKNILAPVNAQQGDNLPVSTFKDMADGTFPQGSAAYEKRGIAVDVPEWKPENCIQCNF
                     CSYVCPHAVIRPIVMTGEEAANAPKGMKMKDMTGMPGYKFAIAPSVLDCTGCGSCANV
                     CPGMKGNKALEMKPLDSQLGVEEDYLYGQKLPVKAEVWAKFKPESVKGSQFKQPLLEF
                     SGACAGCGETPYAKLATQLFGDRMYIANATGCSSIWGGSAPSTPYTVDKRGFGPAWAN
                     SLFEDNAEYGYGIFLAQQHIRDRLEGYLDELHEAKVSSELNASIDNWKATKEDGKANR
                     EATDTLIASLEKIKGNDKVNELIANKDFLAKKSVWILGGDGWAYDIGFGGLDHVIASG
                     QDVNILVFDTEVYSNTGGQSSKASPAGSVAQFAAAGKSTKKKDLAAMAMSYGYVYVAQ
                     IAMGSDYNQCIKAFTEAESYHGPSLIIAYAPCINHGIRGGMGISQTEEKKAVAAGYWH
                     LFRFDPRVADEGNNPFFLDSKVPTASYKDFIMNEVRYSSLTRSFPERAGTLFDAAEKQ
                     AKEKFDHLTRLADK"
     gene            2332..3030
                     /locus_tag="VHR42_00780"
     CDS             2332..3030
                     /locus_tag="VHR42_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX3025756.1"
                     /translation="MSFSEFIVPFVASVGFVTLNEMGDKTQLLAMAFATRFKFWKVML
                     GVLIATILNHGLAVFVGAMLARIPGWQGWVRFIAAALFILFGLWALVSDQLDGDDSGK
                     KTGNEVATVAIAFFFAEMGDKTQLATITLSAQYYGSPLLVLAGTTTGMLLADGLGILI
                     GVVLHQRLPAKALKVMASGAFILFGLVGIWQSLTGTFKFDNNLAATIVSVVALLSIVI
                     GTFLYRKDSKKREV"
     gene            3169..5409
                     /locus_tag="VHR42_00785"
     CDS             3169..5409
                     /locus_tag="VHR42_00785"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX3025757.1"
                     /translation="MTELSENAQIVLEKRYLIRDAKGEPVETIEQLFQRVSTAIAEAD
                     AKFRDDADVEATKENFYNVMTSLEFLPNSPTLMNAGRPLGQLSACFVLPVEDSMEAIF
                     EAIKQAALIHKSGGGTGFSFSRLRPKGSTVNSTGGVASGPISFMRVFNMATEAVKQGG
                     TRRGANMGILRVDHPDILEFIDCKRDNTDITNFNISVGLTESFMNAVINDEEYDLIDP
                     STESVTRSLKAKEVFDKIVESAWQNGEPGIIFLDRLNRDNIVPGQGEIESTNPCGEQP
                     LLPYESCNLGSLNLYRMLIQNEGKWAIDWDKLATVTKTAVHFLDNVIEANRYPLEKIG
                     TTTKLTRKVGLGIMGWADMLLKLGIPYNSDEAVDLADKVMAFITLNARQSSVELAKER
                     GAFPLFEESVLSQGDKVRNATVTTIAPTGTLSIIANCSSGVEPVFAYVFVRNVMDGTE
                     MIEVNPVLREVLEQRGLYSQELMKKIASCGSLEHIPEIPDDIKRVFVSAHDISPEYHI
                     RMQAAFQSHTDNAVSKTVNFENTATRDDVRDVYMLAYHLGCKGVTIYRDGSRDAQVLN
                     IGSVNKGEEKTEEKQGLSPRPRPEVTTGFTEKVKIGCGNLYITVNYDDNGICEVFTNT
                     GRAGGCPSQSEATSRLVSIALRSGMEVDSIIEQLKGIRCPSTIRQKGMKVMSCPDAIA
                     RVIEKVKKYQDGGERMMMPPAAPESGPKNENTSVHMKFCPECGSFLEHEGGCVMCRNC
                     GYSKCG"
     gene            5614..6441
                     /locus_tag="VHR42_00790"
     CDS             5614..6441
                     /locus_tag="VHR42_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3025758.1"
                     /translation="MTGDLHCHTRFSDGSMQPEDLVAYARRAKISCVAITDHDTMEGV
                     GRACESGKNCGVTVIPGVEVSTFDYKRGKKAHLICYQPRNPRLLLEYCAETLRKRHEA
                     SLKIIEKVSRNYPLDLKTVAKYSSENGIIYKQHIIAALMDMGYTLSLFGELFDSILSR
                     KNGWAVFEPEYPDTREAVKLIRESGGFAVLAHPGVYGNFELIGELCKLGLEGLEVFHP
                     RQSDADTRMASEAASRYRLLPTGGSDFHGMYASRVNPLGTRMAGEAAVETLISLGNG"
     gene            6600..6887
                     /locus_tag="VHR42_00795"
     CDS             6600..6887
                     /locus_tag="VHR42_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HEX3025759.1"
                     /translation="MEYTYITQGVCSRAVRFVIEDGIIVSVEFDGGCSGNTQGVGKLA
                     VGMKPEEVIQRLKGIRCGFKETSCPDQLAKGLEEALGLASSEEKAACAEKN"
     gene            6914..7612
                     /locus_tag="VHR42_00800"
     CDS             6914..7612
                     /locus_tag="VHR42_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025760.1"
                     /translation="MEFVLREGVAFMPYIIWLLIIFLFLIVAQEMPFFSHMLETARTD
                     HCYLLEGFREDDSDIKIAGRKIWNSRPVDELSDARELEHQKENGNLEKARGLVKEISE
                     KIIEADGSKPETGDGNARNLSRLMLIFAAFYSVESCIKSGLLQRFILNLIYDGLRVSL
                     PDFYAYLEKSGAFSLYYLCVRRGADVYDCIGRNYAKLVGENTDESIQEEGKSLFSNYF
                     DVTRKMIFSMEFAE"
     gene            7650..9350
                     /locus_tag="VHR42_00805"
     CDS             7650..9350
                     /locus_tag="VHR42_00805"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX3025761.1"
                     /translation="MSCMIEYQRWLAIPSLDEEIKKELESINGNPEMIQSRFGSMLEF
                     GTAGLRGIMGAGLNRMNRFTVRHATQGLANLIDSLGEEAKQRGVAVAYDCRNHSREFA
                     IESAKVLAASGIRSFVFDSLRPTPELSFAVRELHCIAGINVTASHNPKEYNGYKVYWE
                     DGAQLPPDRADVVLKEIRENDIFRDVFVMPLEEAESKGLIRYIGAEMDEKFIQKVLEQ
                     SVCGDAVKKAADEFKIIYTPFHGTGYRLVPEVLKRLGFRNIVPVEEQMTPDGNFPTVK
                     SPNPEDKEGFAIAIELAKRNGIDLIIGTDPDADRVGVIVRNAAGEYLSLTGNQVGVLL
                     TDYVISARREKGTLARDAAVISTIVSTRMTSEICRKNGVSIYEVLTGFKFIGEKIKEF
                     EESGKHTFLFAFEESYGYLSGTYARDKDAVVGSMLIAEMAAWYRAKGMTLYDALEGLY
                     QKYGYFAEKTVSIKMEGMDALEKMKKMMADLRGKTPEEIAGIRVAAVRDYQSGERIET
                     ASLQRSPTGLPKSNVLYYEMEDGSSVIVRPSGTEPKVKLYLLVKAKDKKRSGDSSGKL
                     "
     gene            complement(9514..>9991)
                     /locus_tag="VHR42_00810"
     CDS             complement(9514..>9991)
                     /locus_tag="VHR42_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HEX3025762.1"
                     /translation="VKTQYDYHTTELKIVEEHAYSNGEKAAIKCYGADDVCEGVEIMK
                     KEGVDVSITGNSTNPVRFQHLVAGTYKKWCNDNDKKYFSVASGGGTGRTLHPDNVGAG
                     PASYGLTDTLGRLHGDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVACAVAVAQA"
BASE COUNT         2570 a         2262 c         2761 g         2398 t
ORIGIN      
        1 ataccatcga tggtatcagc attgccaagg aaatcggtct gggcggacgc atcaacacga
       61 ttcttcaggc agctttcttc aaacttgcca acatcattcc ggtagaagat gccgtgaaat
      121 tcatgaagga cgccgcaacg gcttcctatg gcaagaaggg cgaaaagatc gtcgctatga
      181 accacgccgc gatcgaacgc ggtatcgccg gcgccgtcaa gattgacgtt cccgccgact
      241 gggccaatgc gaccgacgaa gatgcttgca acaatgtgga aggcgaccgt caggaacttg
      301 tcagctttgt caagaatatc ctggccccgg tcaacgccca gcagggagac aatcttcctg
      361 tttccacctt taaggatatg gccgacggta cgttcccgca gggttccgcc gcttatgaaa
      421 aacgcggaat cgccgtggat gttcccgaat ggaaacctga gaactgtatc cagtgcaact
      481 tctgctctta tgtttgcccg catgccgtta tccgtcctat cgtcatgacc ggagaggaag
      541 cagccaatgc tcccaagggc atgaagatga aagatatgac cggtatgccc ggctataaat
      601 ttgcgatagc tccttccgtt ctcgactgca caggctgtgg ttcctgcgcc aatgtctgcc
      661 cgggaatgaa gggtaacaaa gctcttgaaa tgaaacctct cgacagccag ctcggcgttg
      721 aggaagatta tctctatggg cagaaactgc ccgtaaaggc ggaggtttgg gccaaattca
      781 aaccggaaag tgtgaaaggc agccagttta aacagccgct gttagagttc tccggcgcct
      841 gcgcgggctg cggtgaaaca ccgtatgcaa agcttgcgac gcagctgttc ggcgacagaa
      901 tgtatattgc gaacgcgacc ggatgttctt ctatctgggg cggttccgcg ccttctactc
      961 cttacacggt cgacaaacgc ggctttggtc ccgcctgggc caactcgctg tttgaagaca
     1021 atgcggagta cggctatggt atcttccttg cccagcagca catccgtgat cgtctcgaag
     1081 gctatctgga tgaactgcat gaggcaaagg tcagcagcga attgaacgct tccattgata
     1141 actggaaggc gaccaaggaa gacggaaagg ccaaccgaga ggctaccgac actctgatcg
     1201 cttcccttga aaaaatcaaa ggcaacgata aagtgaatga gctgattgca aataaagatt
     1261 ttcttgcgaa gaaatccgtc tggatactgg gcggcgacgg ctgggcttat gacattggct
     1321 tcggaggcct ggatcatgtg atcgcttccg gccaggatgt caacattctg gtctttgaca
     1381 ccgaggtgta ttccaacacg ggaggacagt cctccaaggc gagtcctgcc ggctctgtgg
     1441 ctcagtttgc cgctgccggt aagagtacca agaagaagga tctcgctgca atggcgatga
     1501 gctacggtta tgtctatgtg gctcagattg caatgggttc cgattacaac cagtgcatca
     1561 aagccttcac tgaggctgag agctatcacg gcccgtcgct gatcatcgct tacgccccct
     1621 gtatcaatca cggaatcaga ggcggtatgg gtatttccca gaccgaggag aagaaagcgg
     1681 tcgctgccgg gtattggcat ctgttccgct tcgaccctag ggttgctgat gagggcaata
     1741 atcctttctt ccttgacagc aaggttccga ctgcttccta caaagacttc atcatgaatg
     1801 aagttcgtta cagttctctg acccgttcgt tccccgagag agcgggcact ctgtttgatg
     1861 cagccgaaaa gcaggccaag gagaaatttg accacctgac caggctggcc gacaaataaa
     1921 ttttttcgac ctgttataat tccgctccgg aaatgctctg aaccagagca aatcggagcg
     1981 gaattttttt tatccggcct tggccggata attgaggatt gacgatttcc cggcggcatg
     2041 atatactttg aacggttaaa ggggagtagt tgttcctggc tgtcaacata ctggcggcat
     2101 gtttttgaca tgtgcctggc ggcgaggccc gattttgggt aacgagactt ttaacatggc
     2161 cggagtcggc tatgttaaaa gtctttttta atatttcatg aaaaattaat acttgggcag
     2221 ggggtgtaat atgattgtat atccgggtaa tcagcgtaaa atacaggcgt agatacccct
     2281 gttcaaaaca cgaaaaatat ctggaaacac cacagcacag gaggattata aatgagtttt
     2341 tctgaattca ttgtgccgtt tgtcgcaagc gtaggttttg taacgcttaa cgaaatgggc
     2401 gacaaaacac agcttttggc tatggctttt gccacacgtt ttaaattttg gaaggttatg
     2461 ctgggcgttc tgattgcgac aattctgaat cacgggcttg cggtttttgt cggtgccatg
     2521 cttgcccgga taccgggctg gcagggatgg gtccggttta tcgcggcggc gctgttcatc
     2581 ttattcggcc tgtgggcgct ggtaagcgac cagcttgacg gcgatgattc cggcaaaaaa
     2641 acggggaacg aagtggcaac ggtcgccatt gcgtttttct ttgcggagat gggcgacaaa
     2701 acacagcttg ccaccattac gctctcggct cagtattacg gctctccgct tcttgttttg
     2761 gcgggaacca ctaccggaat gctgctggcc gacggtctgg gaatcctcat cggagtggtg
     2821 cttcaccaga gacttcccgc aaaggcctta aaagttatgg cgtcaggagc gtttattctg
     2881 tttggccttg tgggaatttg gcagtccctg acgggaactt tcaaatttga taacaacttg
     2941 gctgctacca tcgtttccgt cgttgcattg ctttcgatcg ttatcggaac ctttttatat
     3001 cgaaaagaca gtaaaaaaag ggaagtttaa acaaaattca gcttaaaata taatgagaga
     3061 tcacatattg acttgcttgg ccttctatta taagatagta ctgttaacac tatatctggt
     3121 gtaaaaatcg ggaaaaataa atatatgtag gttaggcggg taatttaaat gaccgaatta
     3181 tcggaaaacg cacaaattgt attggaaaaa agatatttga tcagggacgc caagggtgag
     3241 ccggtcgaaa cgatcgaaca attgtttcag cgcgtttcaa cagccatcgc cgaagcggac
     3301 gcaaagtttc gggatgacgc ggatgtagag gcaaccaaag agaattttta caacgtaatg
     3361 accagcctgg agtttttacc taattcgccg actctgatga atgccggacg tccgcttggc
     3421 cagctttccg cctgttttgt tctccctgtg gaagacagta tggaagccat atttgaagcc
     3481 attaaacagg cggcgctgat tcataaatca ggcggcggaa cgggtttttc tttttcccgg
     3541 ctgcgtccca agggcagcac ggtgaattcg accggaggag tagcctcggg cccgatttca
     3601 tttatgcggg tgtttaatat ggcaaccgaa gcggtcaaac agggcggaac aaggcgcggc
     3661 gcgaatatgg gtattctgcg ggtcgatcat ccggatatat tggagtttat cgactgtaag
     3721 cgggataata cggatattac caactttaat atctcagttg gattaacaga aagttttatg
     3781 aatgcggtaa taaacgatga ggaatatgat ttaattgatc cttccaccga gtcggtaacc
     3841 agatcgctca aggcgaaaga agttttcgat aaaattgttg agtctgcctg gcagaacgga
     3901 gaaccgggaa tcatattttt ggaccggctc aaccgggata atatcgtacc cggccagggc
     3961 gaaatcgagt ctaccaatcc ctgcggcgaa cagcctcttc tgccttatga atcctgcaac
     4021 cttggttcgc tgaatcttta tcggatgctg attcaaaacg aaggcaaatg ggcgattgac
     4081 tgggacaagc ttgccacggt tacaaaaacc gccgtccatt ttcttgacaa tgttattgaa
     4141 gccaatcgtt acccgctcga aaagatcgga accacaacca aactgaccag gaaagtcggc
     4201 cttggcatta tgggctgggc ggatatgctg ctgaaactcg gcatccccta taactctgat
     4261 gaagcagttg atcttgccga caaggtcatg gcgtttatca cgcttaacgc gcggcagtcc
     4321 agcgtggagc ttgcgaaaga gaggggagct tttcctctgt ttgaggaaag cgttctttct
     4381 cagggggata aagtccggaa cgcaacggtg acgacgatcg ctccgaccgg aacgttatcc
     4441 attattgcca actgttcgag cggcgtagaa ccggtttttg cctatgtctt tgtccgaaat
     4501 gtaatggatg ggactgagat gatcgaggtg aatcctgttt taagagaagt cctggagcag
     4561 cgcgggctgt attcacagga actgatgaaa aaaatcgcct cctgcggcag ccttgagcac
     4621 ataccggaaa taccggatga tattaaaagg gtgttcgtca gcgcgcacga tatttctccg
     4681 gagtatcata tcagaatgca ggcggcgttc cagagccata ccgataatgc ggtatcaaaa
     4741 accgtcaatt ttgaaaacac agctacccgg gatgatgtga gagacgttta tatgctggct
     4801 tatcatctcg gctgcaaggg agttaccatc taccgtgacg gcagccggga tgctcaggtg
     4861 ctcaatatcg ggtctgtcaa caagggtgag gaaaaaaccg aagaaaaaca gggtctctct
     4921 ccccgcccca gaccggaggt cactaccggt tttacggaaa aggttaagat tggctgcgga
     4981 aatctttata tcactgttaa ctatgacgac aacgggatct gcgaggtatt caccaatacc
     5041 ggccgggcag gcggatgccc gtcccagagc gaggcgactt caagactggt atctatcgct
     5101 ttgcgctcgg gtatggaagt cgacagcatt atcgagcagc tcaaaggaat ccgctgtccg
     5161 tcaaccatcc gccagaaggg catgaaggtc atgtcctgcc cagacgccat tgcaagagtg
     5221 attgaaaagg tcaagaaata tcaggacggc ggcgagcgca tgatgatgcc gccggcggcg
     5281 cccgaatcgg gccctaaaaa tgaaaatacg tcggttcaca tgaaattctg tcccgaatgc
     5341 ggaagctttc tcgaacatga gggcggctgt gtgatgtgcc gcaactgcgg atattccaag
     5401 tgcggttaat taagagatat cggaacggat gccgggcaga gcgcccggct atctgttttt
     5461 ttgcctcggc ggatcaaacc gtcgggatga gttacaataa tgtagaacga acccgattga
     5521 aagaacagga aaaataaagt ataatgaatg ggaaatattg atgcggccgg ttccccggaa
     5581 gggcctccgg cttttccaga aaagaggact cagatgacgg gtgaccttca ctgtcatacc
     5641 agattttccg acggttccat gcagccggag gatttggtcg catacgcccg gcgggcaaaa
     5701 atcagctgtg tggccatcac ggatcacgat acgatggaag gggttggaag agcctgcgaa
     5761 tccgggaaaa attgcggagt caccgtgatc cccggagttg aggtgtcgac tttcgactat
     5821 aaaagaggga aaaaggcgca cctgatctgt tatcagcccc gaaaccccag acttttgctt
     5881 gaatattgcg cggaaacgct tcggaaaaga catgaggcgt ctctgaagat cattgaaaaa
     5941 gtttcccgga actatccgct tgatctcaaa acggtcgcaa aatattccag cgagaacggg
     6001 attatctata agcagcatat tatcgcggct ctgatggaca tgggttatac gctgtctttg
     6061 ttcggtgagc tgtttgattc aattttatca cgaaaaaatg gatgggcggt ttttgaaccg
     6121 gagtatcccg acacacggga agcagtaaag ctgattcggg aatcaggagg cttcgccgtg
     6181 ctggcgcatc ctggcgttta cggtaatttt gagttgatcg gagagctttg caaactgggg
     6241 cttgaagggc tggaggtttt ccacccgagg caatcggatg cggatacccg aatggcctcg
     6301 gaggctgctt cccgctatcg tttgctgccg acgggaggct cggattttca tggaatgtac
     6361 gcttcgcggg tcaacccgct cgggacaaga atggccggtg aagccgcagt ggaaacgctg
     6421 atttcgctgg gaaatggata gaaagcggcc ccgtggctgc ccttgtctgc aaattccgct
     6481 tgatgtataa taagagatca gacagggcag tatcctgaat ggatgaattg ccgcgtcaac
     6541 cgatgtgccg gagtcttatt gatcgatctg ccgggcgggt ggaaatggag ggatcaaaca
     6601 tggaatatac ctatattaca caaggcgtct gctcacgggc ggttcgcttt gtaattgaag
     6661 acggaattat tgtaagtgtt gaatttgacg ggggatgctc gggtaatacc cagggtgtcg
     6721 gcaaactggc cgttggtatg aagcccgaag aagtcattca aagactgaaa gggatccggt
     6781 gcggttttaa ggagacttcc tgtccggatc agttggcaaa aggtctggaa gaggctcttg
     6841 gcctggcttc ttcggaagaa aaagcggcgt gcgcagagaa aaattgaatt tttgaaagga
     6901 ggcattccgg gttttggagt tcgttttgcg ggagggtgtt gcttttatgc cgtacattat
     6961 ctggcttctc atcattttcc tgtttctaat tgtcgcacag gaaatgcctt ttttttcgca
     7021 tatgctggag acggccagaa cggatcattg ctaccttctg gaagggttcc gggaggacga
     7081 cagtgatatt aagattgcag gccggaaaat ctggaacagc aggccggtgg atgaactgag
     7141 cgacgcgcgg gaactcgagc atcagaaaga aaacgggaat ctggaaaagg cccgcggtct
     7201 ggtgaaagaa atttctgaaa aaataattga ggcagacggg tcaaagccgg aaactgggga
     7261 tggcaatgcc cggaacctga gtcggctgat gctcattttt gccgcctttt attcagtcga
     7321 gagctgtatt aaaagcggat tgctccagag atttatcctc aatctgatct atgacggcct
     7381 ccgggtttcg ctgcctgatt tttatgctta tctggagaag tccggagcgt tttcactcta
     7441 ttacctttgt gtcaggcgcg gcgcagatgt ttacgactgt atcggcagga actatgcgaa
     7501 gctcgtgggg gaaaacacgg atgaaagcat acaggaagag ggaaaatcat tattttccaa
     7561 ttattttgat gttacgcgca aaatgatttt ttcaatggaa ttcgcagaat aaaaaaataa
     7621 gatgttatcg gatatcggag gtcaaaaata tgagctgtat gattgaatat cagcgctggc
     7681 ttgcaattcc ttcattagac gaggaaataa aaaaagagct ggaatccatt aacggaaatc
     7741 ccgaaatgat ccagtcgaga ttcggatcga tgctggagtt tggaaccgcc ggccttcgcg
     7801 gcattatggg cgcggggctc aaccggatga atcgcttcac cgtccgccac gcgacgcagg
     7861 gtctcgccaa cttgatcgat tctctcggcg aggaggccaa acaaaggggt gtcgcggttg
     7921 cctatgactg ccgcaaccat tcccgtgagt ttgcaattga gtcggcaaag gtgcttgctg
     7981 cgtcgggcat ccgctccttt gttttcgatt cgctccgtcc gactccagag ctgtcgttcg
     8041 cagtcaggga gcttcattgt atcgccggaa tcaatgtcac agcaagccat aatccgaagg
     8101 aatacaacgg ctataaggtc tactgggaag atggcgcgca gctgcctccc gaccgcgcag
     8161 atgttgtgct taaggagatc agggagaacg atattttccg ggacgtcttt gttatgccgc
     8221 ttgaagaagc agaatcaaaa gggctgattc gttatatcgg cgccgagatg gatgaaaagt
     8281 ttattcaaaa agttctggaa cagtcggtct gcggtgacgc agtcaaaaag gctgccgatg
     8341 agtttaagat tatttataca ccctttcatg gaacaggtta ccggcttgtt cccgaagttc
     8401 tcaaaagact tggatttcgg aatattgtcc cggttgaaga gcagatgacc ccggatggca
     8461 acttcccgac ggtgaagtcg ccaaatcctg aggataagga aggcttcgcc attgcaatcg
     8521 aattggcgaa aagaaacgga atcgacctga ttatcggcac agatcccgac gccgaccggg
     8581 ttggcgtgat tgtccgcaat gcggccggag aatatctcag cctgacgggg aaccaggtag
     8641 gagtgcttct gaccgattat gttatttctg cccgcaggga aaaaggaacg cttgccaggg
     8701 acgcggcagt catctcgacc attgtttcta ccagaatgac ttccgaaatc tgccggaaaa
     8761 atggcgtttc tatttacgaa gtgctgaccg ggtttaagtt tatcggtgaa aaaatcaaag
     8821 agtttgaaga atccggaaaa catacgttcc tgtttgcttt tgaagaaagt tacggctatc
     8881 tttccggaac atacgccaga gacaaagatg cggtggtcgg ctcgatgctc atcgcggaaa
     8941 tggcagcgtg gtaccgtgcc aaaggcatga ccctctacga tgctctcgag gggctctatc
     9001 agaaatatgg atattttgcg gaaaaaacag ttagcattaa gatggaaggc atggatgccc
     9061 tggagaaaat gaagaaaatg atggcggatt taaggggcaa gacaccggaa gaaatcgcag
     9121 ggattcgtgt cgcggccgta agggattatc agagcggcga gcggattgag acagcgtcgc
     9181 ttcaaagatc cccgaccggg ctcccgaaat cgaatgtgct ttattatgaa atggaagacg
     9241 gaagttcggt aatcgtccgc ccgtccggaa ccgagccgaa ggtgaagtta taccttctgg
     9301 ttaaggcaaa agacaaaaaa aggagcggag actcttctgg aaagctatga ccgtggtttc
     9361 aagagcttga tcggccgtta aaaaaggcga ctgttctttc aaaagaattg agccccccgc
     9421 gaatgaatcg cggggggctc aatctgtgga tcaaaagagg gaaaatccga agaaactgcg
     9481 tttaaaagga ttgcccctct taaataaatt tgattaggct tgagcaaccg caaccgcgca
     9541 ggcgacagtc gcgccaacca tcgggttatt gcccattccg atcagaccca tcatctcaac
     9601 gtgggccgga acagaggacg aaccggcgaa ctgggcgtcg ccgtgcaggc ggccgagcgt
     9661 atcggtcaga ccgtaagaag ccgggccggc tcccacgtta tcaggatgga gtgtgcggcc
     9721 ggtgcctccg ccggaagcaa ccgagaaata ttttttgtca ttgtcgttgc accatttttt
     9781 ataggtaccg gcgacaaggt gctggaagcg aacggggttg gtggagtttc cggtgatgga
     9841 aacgtcaacg ccctcttttt tcatgatctc aacgccttca cagacatcgt cggcgccgta
     9901 gcatttgatg gcggcttttt cgccgttgga gtaagcatgc tcctcgacga tcttgagctc
     9961 ggtggtgtgg tagtcatatt gggttttgac a
//
LOCUS       DATALY010000023         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_264800,
            whole genome shotgun sequence.
ACCESSION   DATALY010000023 DATALY010000000
VERSION     DATALY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264800"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1893)
                     /locus_tag="VHR42_00815"
     CDS             complement(<1..1893)
                     /locus_tag="VHR42_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX3025763.1"
                     /translation="MANGKSAVKTRGRPAGKKKSSVRVVKDKSVGQKDARKIGRQAGA
                     ILLFAAALLLASIILIPGVNAWKFFHNLCFGIFGPCAFLWPVVLIYIAVMTAKDCFSS
                     STHVKLLEALGLAVLICSFLHIFMIGSFPRAGITELYQKGITLKSGGVAGGMLGAPLY
                     LLFGKTGAAITVFLLIFVFTLLVTGVTLGSLFNWAAAPIRKLRQRTGQTEESDSEPAA
                     IFFKHKKPKYNIDVDLGPENQAEPEEVPESEKPAQTVETEKDKPPAALDDLVKKISNF
                     NQEEAPKIREEIPAMETPDGQQVLYTYPPVTLLKQETRPITQDVSEELKSNAARLVDT
                     LKSFGVETRITDICRGPAVTRYELQPSAGVKISRITSLADDIALNLAAAGVRIEAPIP
                     NKPAVGIEVPNKAVSIVHIREIVDSKEFRDARSRLTFALGRDIAGNVAVADIAKMPHM
                     LIAGATGSGKSVCINSIIISLLYKGTPDEVKFLMVDPKVVELGIYNGIPHLLVPVVTD
                     PRKAAGALNWAVTEMLNRYKLFADNNVRDMKGFNALAVKTEGLTPMPHIVIIIDELAD
                     LMMAAPNDVEDAICRLAQMARAAGMHLVIATQRPSVDVITGVIKANIPSRIAFAVSSQ
                     VDSRTIL"
     gene            complement(2161..2424)
                     /locus_tag="VHR42_00820"
     CDS             complement(2161..2424)
                     /locus_tag="VHR42_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HEX3025764.1"
                     /translation="MLLYTIMSYEDIFSESYRPEIQTAKIKGGYLEYVKSGGDYSVSR
                     LISTDPRMYLNARIAPGARFGFHSEALRLPSASEKDTNPLYKG"
     gene            complement(2426..3289)
                     /locus_tag="VHR42_00825"
     CDS             complement(2426..3289)
                     /locus_tag="VHR42_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX3025765.1"
                     /translation="MTEDKDMDQARCQEEEEDKNEELDARTQQIVESGSITMSDGHHL
                     IHCLTIIGQVEGHFILPPQNKTTKYEHVIPQIVAIEEDPNIEGLLIILNTVGGDVEAG
                     LAIAELVAGMNKPTVSLVLGGGHSIGVPLAISARKSFIAPTATMTIHPVRMNGIVVGV
                     PQTLNYFDRMQDRIVNFVTLNSHITAERFKKLMMNTGELVMDVGTVLDGAAAVREGLM
                     DSLGGLADAIHCLHEMIEEGKPKDQQGEKDGEEEKPEKSKEKSKAGKKSVHKRNSQEK
                     KVSRKKGGGQK"
     gene            complement(3423..>3913)
                     /locus_tag="VHR42_00830"
     CDS             complement(3423..>3913)
                     /locus_tag="VHR42_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HEX3025766.1"
                     /translation="PLKKSLFAYVVKLLKKRQQELIDIIKEYPKFRAPTNYAHIKQMV
                     TDYLGYGNKMGEGWLLTGEMLELINSGVENIVCAQPFGCLPNHVVGKGMIRKIKQNYP
                     SSNVVAIDYDPGATRINQENRIKLMLANARMTTAKTASAKETEPVSAADQKPAVAYAK
                     QF"
BASE COUNT          879 a         1104 c          937 g          993 t
ORIGIN      
        1 cagtatggtg cgtgaatcca cctgggaaga gacggcaaaa gcgattctcg atggtatatt
       61 ggccttgata acgccggtaa tgacgtcgac cgacggccgc tgggtggcaa tcacaagatg
      121 catgcccgcc gcccttgcca tctgggccag cctgcagatc gcgtcttcca catcattggg
      181 ggcggccatc atcaggtcgg ccagctcatc aatgataatc acgatatgcg gcatcggggt
      241 cagaccctcc gtttttacgg cgagagcgtt gaagcctttc atatccctga cgttgttgtc
      301 tgcaaacagc ttgtagcggt tgagcatctc ggtgacggcc cagttcagcg ctccggcagc
      361 cttgcgcgga tcggttacta ccggaaccag cagatgcgga atcccattat agattccgag
      421 ctcgacaact ttcgggtcaa ccatgagaaa ttttacctca tccggcgttc ccttatacaa
      481 aagactgatg ataatggaat tgatgcagac cgattttccc gagccggtag cgcccgcgat
      541 cagcatatgc ggcattttgg caatatccgc cacggcaaca ttcccggcaa tatcccggcc
      601 cagcgcaaag gtcagacggc tgcgcgcatc acggaattcc ttcgagtcga cgatctcgcg
      661 gatatgcacg atgctgaccg ctttattcgg cacttcaatg cccacggccg gcttgtttgg
      721 aatcggagcc tcaattcgga ctcccgccgc cgcaaggttg agcgcaatgt cgtcggcgag
      781 agacgtaatc ctgctgattt taaccccggc cgacggctgg agctcatagc gggtgaccgc
      841 gggtcctcgg cagatgtcag tgattcgtgt ctcaacacca aagcttttta acgtatcgac
      901 cagcctggcc gcgttggatt tcagctcttc ggaaacatcc tgggtgatcg gccgcgtttc
      961 ctgtttgagc agcgtcaccg gggggtaggt gtagagcacc tgctggccgt ccggggtctc
     1021 cattgcggga atttcctcac ggattttagg agcttcctct tgattaaaat tcgaaatctt
     1081 tttgaccaga tcatccagag ccgccggcgg cttatctttt tcggtttcga ccgtctgagc
     1141 aggtttttcc gactccggaa cctcttccgg ctccgcctga ttttcgggtc cgaggtcgac
     1201 gtcaatatta tattttggct ttttgtgttt gaaaaaaatt gcggccggct cgctgtctga
     1261 ttcctcggtc tgaccggttc tctgccttaa tttccggatc ggcgcggccg cccagttaaa
     1321 aagagagcca agcgtaacgc ccgttacaag aagggtaaat acaaatatca gcagaaacac
     1381 cgttatggcc gctcccgttt tgccaaacag cagataaagc ggcgccccaa gcattccacc
     1441 ggccacaccg ccgcttttca gggtgattcc cttctgataa agctcggtga ttcccgctct
     1501 tggaaatgac ccgatcataa aaatatgaag gaaggagcag atgagcaccg ccagccccag
     1561 cgcctccaac agtttcacat gcgtggaaga ggaaaaacag tcttttgcgg tcatgaccgc
     1621 tatgtaaatc aaaaccacag gccaaagaaa agcgcagggc ccgaagatac caaagcataa
     1681 attgtgaaag aatttccagg cattcacacc gggaattaaa ataattgaag ccagcagcag
     1741 cgcggcggca aaaagcaaaa ttgctccggc ctgcctgccg attttccgag cgtccttctg
     1801 cccgaccgac ttatctttca caacccggac cgaagacttt tttttaccag ccggccttcc
     1861 cctggtctta accgcagatt tgccgttcgc catcattcat caccttttcc catccttttt
     1921 cacctgcgtg ggccgtcccg cgcaggtgaa accggccaaa ttgccggctt ttattgtcca
     1981 tccttcaata taacacagcc gatcgacgaa aaaacagaaa aaaacacgaa tccgaccgtc
     2041 cgtccgacag ccgccgataa gcggaataaa aagcgccgaa cgatctcttc ggcgcaggat
     2101 gccacggcgg ctggatcaat taaaatacta tctctatttt atagtcggag cccgtttatg
     2161 tcaacctttg taaagcggat ttgtgtcttt ctccgaggcg gacggcagcc ggagcgcttc
     2221 cgaatgaaat ccgaatcttg ctccgggcgc aatcctggcg ttgagataca ttctcgggtc
     2281 ggtcgaaatc agtctggata ccgaataatc ccctcccgac ttcacgtatt ccagataacc
     2341 gccctttatt tttgccgtct gaatttcggg tctgtagctc tcggagaaaa tatcttcata
     2401 agacatgatc gtgtataaaa gcattttatt tttgcccgcc gcctttcttc cgggaaacct
     2461 ttttctcctg gctgttcctt ttatgaacgg atttttttcc cgcctttgac ttttcttttg
     2521 atttctcggg tttttcctcc tcgccgtcct tctccccctg ctgatccttg ggtttcccct
     2581 cctcgatcat ctcgtgaaga cagtgaatcg cgtccgcaag ccctccgaga ctatccatca
     2641 gcccctctct gacagcggcc gcgccgtcaa gaacagttcc gacatccatg acgagttcgc
     2701 ctgtattcat catcagcttt ttaaaacgtt ccgccgtgat atgagaattc agcgtcacaa
     2761 aatttacaat acgatcttgc atccggtcaa aataattcag cgtctgggga accccgacta
     2821 caattccgtt cattctcacc ggatgaatgg tcatggtcgc ggtgggagcg ataaacgact
     2881 tccttgccga tattgcaagc ggaacgccaa tggagtgtcc gccccccaaa accagagaaa
     2941 cagtcggctt gttcattccc gccaccagct ccgcgatggc aaggccggct tccacatcgc
     3001 ctccgaccgt gttgagaatg atcagcagcc cttcgatatt ggggtcctcc tcaatagcga
     3061 cgatctgggg aatcacatgc tcatatttgg tcgtcttatt ctgcggaggc aagataaaat
     3121 gtccctccac ctgaccgata atcgtcaggc agtgaatcag gtgatggccg tccgacatcg
     3181 tgatagagcc ggattccaca atctgctggg ttcgggcatc caactcctca ttcttgtctt
     3241 cttcctcctc ctggcagcga gcctgatcca tatccttgtc ttcggtcatg caatcccatc
     3301 cttgtccgcg tcagcatccc gcgtcagcgc aaaataatac cgcctccgca actgaaaaca
     3361 gaagcagaaa cggtattatt gttgccggat tatcggcagg tatgcttacg ggccttttca
     3421 aatcagaatt gtttcgcata ggcgaccgca ggtttttggt cggctgccga cacgggttca
     3481 gtctctttcg ccgaggcggt tttcgcggtt gtcatccgcg cgttcgcgag catcagtttg
     3541 atcctgtttt cctgattgat tctggtcgcg cccgggtcgt aatcaatggc cacaacattg
     3601 gacgatggat agttttgttt gattttccgg atcataccct tgccgacaac atggtttgga
     3661 agacagccga aaggctgtgc gcaaacgata ttttcaacac ccgaattgat gagctccagc
     3721 atttcacccg tcagaagcca gccctcgccc attttattcc cgtagcccag ataatcggtc
     3781 accatttgct taatgtgggc ataattggtg ggggccctga atttcgggta ttcctttata
     3841 atgtcaatca actcctgctg gcgtttcttc agcagcttca caacatacgc aaagagtgat
     3901 tttttcagag gat
//
LOCUS       DATALY010000024         9896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_4296,
            whole genome shotgun sequence.
ACCESSION   DATALY010000024 DATALY010000000
VERSION     DATALY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4296"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VHR42_00835"
     CDS             <1..644
                     /locus_tag="VHR42_00835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX3025767.1"
                     /translation="QSINGRHTDTYPNPEDYDLMFVFGGSQHVWDKASDPWLTDEIRF
                     VSHALASGKPVIGFCLGSQIISEALGGRVFQSATKETGFYQINIKTEGETTLMKGLPN
                     PFRSLLLHSDHYTLPTGCICEAFTPASPNQAFYSEVAPAVGFQFHPEYTKEKVRYFLE
                     VIPDGWHIESGPCSREAVLQSLDSRPDTIGLFQILIENAIRLFNDRFHLELKS"
     gene            complement(748..960)
                     /gene="copZ"
                     /locus_tag="VHR42_00840"
     CDS             complement(748..960)
                     /gene="copZ"
                     /locus_tag="VHR42_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEX3025768.1"
                     /translation="MAKQTSILHVDGMSCSHCENAVKKAVGSLPGVEKVTVDLPGKTV
                     TVEYEDGKVTLREMEDAITDQGYDVQ"
     gene            complement(981..3437)
                     /locus_tag="VHR42_00845"
     CDS             complement(981..3437)
                     /locus_tag="VHR42_00845"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3025769.1"
                     /translation="MLRKETIPISGMTCASCAARIEKGLARLEGVRSAGVNFAVETAT
                     VEYDDSLVERGQIVQLVSKLGYSVIEEKDPNLSKAVLAISGMTCAACSARIEKKLNRL
                     DGVSKAAVNLTTEKATVEYSAEKLNVPDLVKAVENLGYGARKIGGAGADREKQQRDRQ
                     RKRLLYTFLCSAALSSPLLLAMILPVFGIHVLVLHNPYFQLAIATPVQFVIGFRFYRN
                     AFYSLKSGGANMDVLVSMGTSAAYFFSLYHVFAGSEGADGMNHLYFEASAVIITLVLL
                     GKYLESAAKGKTSEAIKKLMGLRAKTARVIRQGTERDIPVEQVEVGDTVIVRPGEKVP
                     VDGRILEGNSSVDESMLTGESLPVEKKAGDSCIGGTINKVGTFRFEATQIGRDTVLSQ
                     IVQMVEDAQGSKAPIQKIADRVSGIFVPAVAGIALLTFFGGLIFANDLTRAVVSAVAV
                     LVIACPCALGLATPTAIMVGTGKGAQNGILFKGGEHLEIACRISAVVFDKTGTITSGR
                     PEVTDVIALGELNENEVLTLAASAEKDSEHPLGTAIYEKGKKELSTVSDPEAFEAVPG
                     KGVTAVTGGRRIEIGTRNFLKEKEIDLSPLEDQLARLESQGKTAMIMAVDGKIEAIIA
                     VADTIREHSREAVSELRKMGVEVYMLTGDNARTAAAIAGQAGISHVLADVLPEHKARE
                     VEKLKQAGKTVAMVGDGINDAPALATSDVGMAIGTGTDVAIEAADVTLMRADLRAVPA
                     AIRLSRQTMRKIRQNLFWAFFYNSIGIPFAAFGMLNPMIAGAAMAFSSVSVVTNSLSL
                     NAYHPFPKLKTGKAIESETK"
     gene            complement(3614..4057)
                     /locus_tag="VHR42_00850"
     CDS             complement(3614..4057)
                     /locus_tag="VHR42_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3025770.1"
                     /translation="MKISTKGRYGLRAMLDLAVFSNGNQVSLFNIAERQNISVNYLEQ
                     VFSSLRKAGLVRSVKGSQGGYILVSPPEEIKVGDILRCLEGNLKVVDEEPDNSGTQSG
                     IMLTCIKQAVWDKINQSIDRVVDSVTLANLTEEYRNTDNQPMFYI"
     gene            4301..4927
                     /locus_tag="VHR42_00855"
     CDS             4301..4927
                     /locus_tag="VHR42_00855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX3025771.1"
                     /translation="MIQVEKDGGVVIRAPSSLSVNSLEKFIQGKQTWIRNAVERQKKH
                     HLKTSLSIRDGGRLLYLGELRPVVSDSGVRDAYYQEGTYYIPTLSEEENRQSVISLLR
                     KTAKRTFTRRVKYFSCLAGIRYSSIRITGAATRWGSCNAKGGMNFSYRLLFTPPETID
                     YVIVHELSHILQHNHSEAFWKVVENIMPDYRRRARALRNFEKEYDFSV"
     gene            5065..5724
                     /locus_tag="VHR42_00860"
     CDS             5065..5724
                     /locus_tag="VHR42_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX3025772.1"
                     /translation="MSLDRIELHKYSLAEELISSISHGVGAALSIAALVVGLVFSGLY
                     NDIWCMVSMAIYGSMLILLYLMSTIYHGLRPNSGKKVFRVLDHCSIFLLIAGTYTPYM
                     LVTLRGPLGWTMFCVVWGVSILGIVLNAVDLIKFAKFSMVCYLAISWAIIIVIKPLIN
                     HIKFGGILLLLLGGVAYTVGAVLYGIGRRKKYVHSVWHFFVLAGSILHYFSILFYVVM
                     A"
     gene            complement(5793..6008)
                     /locus_tag="VHR42_00865"
     CDS             complement(5793..6008)
                     /locus_tag="VHR42_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025773.1"
                     /translation="MQDQREKMAHRSEEDMMQFSPAGVGRVKKEIRSVLKMMEISAEP
                     GGDREKKGTGNSRESKRRIGNNDLKQH"
     gene            complement(6321..7136)
                     /locus_tag="VHR42_00870"
     CDS             complement(6321..7136)
                     /locus_tag="VHR42_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3025774.1"
                     /translation="MNQNEDFIKLVVENNLINTLFQPVVDLSDGNIIGYEAFSRGPEN
                     SNMYSPLSMIYEAKNRKMIGELDTLFRKDALENAAKQGMKRLLFLNVDPDVIYQGELL
                     TDSGEEELDIPNSSIVIEITERSNLCNFNTFSQSIKRYKEAGFKIALDDIGSGFSELD
                     CVSKIGPDFVKIDNILINGMDQDAKKQDTVQSIIKLSEITGSKIIAEGVESEKELLEL
                     LKLGVYAAQGKYFGTPETDIKGVNPDAVNCINQYYKEKYDQELKNLIDETASE"
     gene            7346..9040
                     /locus_tag="VHR42_00875"
     CDS             7346..9040
                     /locus_tag="VHR42_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HEX3025775.1"
                     /translation="MKVVLAALNSKYIHMSLAPWYLKSYSGRDSIRVMEFTVNQDAGD
                     ILRDIYREKPDVLGFSCYIFNIQTVEQILEDIKKVMPEIKIILGGPEVSFDPRDVLTR
                     CKAVDYVICSAGEIPFKKLLDELESGREPEKIGGVALRAGNGILCNPPDVPLSRSLAA
                     IPSPYTDEMLAQAKGKILYFETSRGCPFHCSYCLSSESGGVRYFPMERVRRDLKTIAL
                     SEVRQVKFVDRTFNCNSSRAKEIFRYLIDLSQSLGENHFKNYHFEAAADLFDDEMIEL
                     LSHAPEGLFQFEIGIQSFNEPTLDACGRKTDLSVCANYISRLLEAGNIHIHLDLIAGL
                     PLEDLPTFAHSFNSVYALRPHCIQLGFLKLLKGSGMRRDARRHSIDYSARPPYEVLQT
                     ASMSFDDLIFLKSIDFCVDRLYNSEKFSQSLKFLISRSESPFRLFSDFSAFLNGKPEN
                     GISLKNLYGLLYHFAEQSLRPDDLDVFNEFIKYDYFSSDNSCNPPREIKRCIDPQIKQ
                     LYAGLKPGGERVHFERFEIDPLDSTKKFPERKETVLRFDYGRRNRVTGLYGVTPIS"
     gene            complement(9267..>9896)
                     /locus_tag="VHR42_00880"
     CDS             complement(9267..>9896)
                     /locus_tag="VHR42_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX3025776.1"
                     /translation="LSAIREFTEFGSGFQIALRDLEIRGAGNILGSQQHGHMEAVGYD
                     MYLKLLNEAVLEEKGEKPASAGECMVDLQISAHISENYIESSNLRLETYRRIASILDE
                     EDASDVIDEMIDRYGEPPVEVKSLVDVALIRNTAASLGIREISQRESMILVFPEKLDF
                     KAVSGLVTRLRGRVMVNAGQKPYVSVKMKTGESVIDTIRETLESLKAES"
BASE COUNT         2377 a         2611 c         2214 g         2694 t
ORIGIN      
        1 ttcagtctat taatggcaga catacggata cttacccgaa cccggaagac tatgacctga
       61 tgtttgtatt cggcgggtcc cagcatgtct gggataaagc atccgaccct tggctcacag
      121 acgaaatccg ttttgtcagc catgcgctcg ccagcgggaa acccgtcatc ggtttctgcc
      181 tcggcagcca aattatttcc gaagccttgg gcggaagggt tttccaatcc gcaacaaagg
      241 aaaccggatt ctatcaaatt aacatcaaga cggagggaga aaccacgttg atgaaagggc
      301 tgccaaaccc tttccgttcg ctgctcctcc actccgacca ttacactctc cccacaggct
      361 gtatttgtga agcgtttacg cctgcttctc cgaatcaggc gttttacagc gaggtcgccc
      421 ccgctgtcgg gttccagttt caccccgaat acaccaaaga aaaagttcgt tattttctcg
      481 aagtgattcc agacggatgg catattgaaa gcggtccctg ctcgcgggaa gcagtacttc
      541 aaagccttga cagccgtccc gataccattg ggctgtttca aattctcatt gaaaatgcca
      601 tccggctatt taatgatcgt tttcacctgg agcttaaatc ataatacgat tcatttgatt
      661 ggcaatcgaa aaggtggaaa catccgcaac gggatgtttc cacctttttc aacgcgccga
      721 cggtctgaaa gcaggccgct ttcttattta ttggacgtca tagccctgat cggtgatggc
      781 gtcttccatt tcgcgaagag tgaccttccc gtcttcatat tccaccgtta cggtcttgcc
      841 cggcagatca acggtgacct tctccacacc gggaagcgat ccgaccgcct ttttgacggc
      901 gttctcgcaa tggctgcagg acattccatc cacatgcagg atactggttt gttttgccat
      961 gataattcct ccaatgaact ttattttgtt tccgattcta tcgctttacc cgtctttaac
     1021 ttaggaaacg gatgataggc attcaagctg agcgaattag tcacaaccga aacggagcta
     1081 aacgccatgg ccgctccggc aatcatcggg ttcagcattc caaacgccgc aaacggtatg
     1141 ccgatgctgt tatagaaaaa ggcccagaac agattttgcc ggatttttct catcgtctgc
     1201 ctggaaagcc ggatagcggc gggcaccgcc ctcagatcgg ccctcatcag cgtgacgtcg
     1261 gccgcttcaa tggcgacatc cgttcctgtg cctatggcca ttccgacgtc tgatgtggca
     1321 agggcgggcg cgtcgttgat cccatccccc accatggcga cggtcttgcc ggcctgtttg
     1381 agtttttcca cttcccttgc cttatgctcg ggcagaacat cggcaagaac atggctgatt
     1441 ccggcctgtc cggcaattgc cgcggctgtc cttgcgttat cgcctgtcag catataaacc
     1501 tctacgccca ttttccgcag ttcgctgaca gcctcccggg aatgttcgcg gatggtgtcc
     1561 gctaccgcaa tgatcgcctc tattttaccg tcaaccgcca taatcattgc agttttcccc
     1621 tggctttcaa gccgggccag ttgatcctcc aacggactta aatcgatttc tttttccttc
     1681 aggaaatttc tcgttccgat ttcaatccgg cggccgcctg ttactgccgt cacgcccttt
     1741 cccggaaccg cctcaaaagc ttccggatca gaaaccgtag aaagttcctt tttccccttt
     1801 tcataaatcg cggttcccag agggtgttcg gagtcttttt ccgccgatgc ggccagcgtt
     1861 aaaacttcat tttcattcag ctcgcccaaa gcaattacgt cggtgacttc cggccgcccg
     1921 ctggtaatgg ttccggtttt gtcaaaaacc acggcgctga tccggcaggc aatctccaga
     1981 tgctccccgc ccttaaacag aatgccgttc tgagcgccct ttcccgtgcc gaccataatg
     2041 gcggtcggcg tagccaggcc cagcgcgcag ggacaggcaa ttacaagcac ggcgacggcg
     2101 ctgacaaccg cgcgggtcag atcattggcg aaaatcagtc cgccgaaaaa ggtcagcaga
     2161 gcgattccag ccaccgccgg aacaaaaatt ccggaaactc tgtcggcaat tttctgaatc
     2221 ggggctttgg agccctgcgc atcttccacc atctgaacga tttgggaaag cacggtatct
     2281 cgcccgattt gggtcgcttc aaagcgaaag gtccccacct tgttgatcgt tccgccgatg
     2341 caggaatccc ccgccttttt ttccaccgga aggctctcgc cagtgagcat ggactcatcc
     2401 acgctcgaat ttccctcaag gattctgccg tcgacgggga ctttttctcc gggccggaca
     2461 atcacggtgt cgccgacctc cacctgttca acgggaatat cgcgttcggt tccctgccgg
     2521 atgactctgg ctgttttcgc ccgcagcccc atcagctttt taattgcctc ggatgttttt
     2581 ccctttgcgg cggattcaag atatttaccg agcagaacca gcgtaatgat gactgccgac
     2641 gcttcaaaat aaagatgatt cattccgtca gcaccttcag agccggcaaa cacatgataa
     2701 agactgaaaa aataggccgc cgaagttccc atggaaacca gaacatccat attcgcccct
     2761 cccgatttga gcgaatagaa cgcattgcgg taaaatcgaa acccgatcac aaactgaacc
     2821 ggtgtggcaa tggccagctg gaaatacgga ttgtgaagta ccaggacatg aattccgaat
     2881 accggaagaa tcatcgccaa cagcaaaggc gagctaagcg ctgcggaaca aagaaaggtg
     2941 tataggaggc gttttctttg ccgatccctc tgctgttttt cccgatcggc cccggctccc
     3001 cctattttcc gcgcgccata gcccagattt tccactgctt tcacgagatc cggcacattc
     3061 agcttttcgg cggaatattc caccgttgct ttttcggtag tcagatttac cgcggctttt
     3121 gatactccat caagccggtt taactttttt tcaattctcg ccgaacaggc tgcgcaggtc
     3181 atgcccgaaa ttgccaaaac agcttttgaa agattcggat ctttttcttc aatcaccgaa
     3241 tatcccagtt tggaaaccag ctgtacgatt tggccgcgtt cgaccagtga atcatcatac
     3301 tccacagtgg cggtttcaac ggcaaaattt acgccggccg accggacgcc ttccagtctg
     3361 gcgagaccct tctcaattct cgcggcacag gaggcgcaag tcatgccgga gatcggtatt
     3421 gtttctttcc gcagcattgt ttcccttctt tcggtatcgc ctgctttttt gtgatcgata
     3481 agcccaggct tccacttttt ctttaattat accacataag tatagtttat gtcatcaaga
     3541 aattcaaccg aaaaaaaaca aaagtctccc tttaacagga gactggattt catccaaaat
     3601 cagaaacatg ggctcagata taaaacatcg gttgattatc cgtgtttctg tactcttccg
     3661 taagatttgc aagcgttacg gaatccacca ccctgtcaat gctttggttt attttgtccc
     3721 aaacagcctg tttgatacag gtcagcatga ttccgctttg cgttccgctg ttatcaggtt
     3781 cctcatcgac tactttcaga ttgccctcca gacaccttag aatatctccg accttaatct
     3841 cttccggggg tgaaacaaga atatagcctc cctgtgagcc ctttacgctc ctgaccagcc
     3901 cggcttttct aagggaagaa aagacctgct ccagatagtt gacggatata ttctgccgct
     3961 ccgcaatatt aaaaagcgat acctgattac cattggaaaa aacagcgaga tcaagcatcg
     4021 ctctcagccc gtatctgcct ttggtagaaa ttttcatact tcgacctcat tcatattata
     4081 ttaatcatat atgtttataa tataatctat ccccccccgt tgtcaacctc atttactaag
     4141 ttttcccgac cggattgtga aacattctga attgaggtat gataggcaat ggaatccaaa
     4201 ggcattggat aaatgaaatg gtggtggttc tacgaaaaga aaaggacgtt caatcagccg
     4261 cgatggttac ccggtcatcc ggtccgaacg aaaaaccata gtgatacaag tagaaaaaga
     4321 cggcggagtc gtgatcagag ctccttcctc attatccgta aattctctcg aaaagtttat
     4381 ccaaggcaaa cagacgtgga tcaggaatgc agtggaacgt cagaaaaaac accatttaaa
     4441 aacaagcctg tcgatccggg acggaggccg gcttctgtac ctcggcgagc taaggcccgt
     4501 ggtgtcggac tccggcgtcc gcgacgcata ttatcaggaa gggacctact acattcccac
     4561 tctcagcgaa gaggaaaacc ggcaatccgt catcagcctg ctcaggaaaa ccgcgaaaag
     4621 aacgtttacc cgcagggtga agtatttttc atgtttggcg ggcatccggt attcctcgat
     4681 tcggataacg ggagcggcca cccggtgggg ctcctgcaat gcgaaaggcg gaatgaactt
     4741 ttcctaccgg ctgctgttta ctcccccgga aaccatcgac tatgtgattg ttcacgaatt
     4801 atcccatatt ctccagcata accattccga agcattctgg aaggtggttg aaaacatcat
     4861 gcccgattac cggcgacggg ccagggcgct gagaaatttt gaaaaagagt atgatttttc
     4921 ggtttaggac tccgataatt tttttaaaaa gtttgattga aattagaaat ttatggttta
     4981 taatgaaagt agactgcacc tatctgattc tcgaacttca aagcatgacg gcgcgtgaat
     5041 attcgcgcag aaatggcggg acttatgtca ctggatcgta tcgaactcca caaatacagc
     5101 ctggcggaag aactgatcag ctctatatcc catggcgtgg gggctgccct ttccattgcc
     5161 gccctggtgg tcgggctcgt tttttccggc ctttacaatg atatttggtg catggtcagc
     5221 atggctattt acggatcaat gctgattttg ctctacctga tgtccaccat ttatcacggg
     5281 ctccggccaa attccgggaa aaaggtattc cgggtgctgg atcactgctc cattttcctt
     5341 ctgatcgccg gaacctacac cccctatatg cttgtgacac taaggggacc tttgggctgg
     5401 acgatgttct gcgttgtgtg gggagtatcc attctcggga tcgttttaaa cgccgttgat
     5461 ctaattaaat tcgccaaatt ctccatggtt tgttatctcg ccatcagctg ggctattatc
     5521 atcgtaataa aaccgctgat aaaccacatt aaattcggcg gaatccttct tctgctgctt
     5581 ggcggcgtag cctatacggt tggcgcggtt ctttacggaa tcgggcggcg gaaaaagtac
     5641 gttcattcgg tgtggcactt ttttgtgctt gcgggaagta ttcttcacta tttttccatt
     5701 ctgttttatg tcgtcatggc ctgaatcttt acagcatgga aaaatcccct gcgcgaatca
     5761 aaacgggccc gaaaaaaccg ggcccgtttt ttttagtgct gtttcaggtc attgtttccg
     5821 atgcgccttt tgctttcccg agaattacct gttccttttt tctcacggtc tccgcctggt
     5881 tccgcagaaa tttccatcat cttgagaacc gatcgaattt ctttttttac tcttcccaca
     5941 ccggcagggc tgaactgcat catatcttct tctgaccggt gagccatttt ctcccgctga
     6001 tcctgcatat ttacgcttcc cttcttttca ttcgaaaccc tacgcaatta ttctgagata
     6061 agaagtgcgt aaatattctc taaaaagcag cggcccggtt taaaaccggg ccgccaaact
     6121 atttttacag aatcagatca ttctgaaaat ttgaaattgc agtaacagaa cgttaacaga
     6181 tccgtcatat atgatggcta taataaaacc agtaagtaat cagctaatcc tcttaataat
     6241 ttgttttcat atatcccctc ctcatacttt ttgtcctatc cgcgggcagc agtcaggctg
     6301 tcggcggaat ttttttgcct ttattccgaa gcggtctcgt cgatcaggtt cttaagctcc
     6361 tggtcatatt tttctttata atactggttg atgcagttca cagcatcggg gtttacccct
     6421 ttgatgtctg tttcgggtgt tccgaaatat tttccctgtg ccgcataaac gccgagcttc
     6481 aaaagctcaa gaagctcttt ttcagattcc acgccttcgg cgataatctt tgatccggtg
     6541 atttcagaaa gcttaataat ggactgcacc gtatcctgct tcttagcgtc ctgatccatt
     6601 ccgttaatga gaatattatc aatcttaaca aaatcgggtc cgatttttga aacgcagtca
     6661 agctctgaaa aacccgagcc tatgtcatcc agcgcaattt taaaaccggc ctctttgtag
     6721 cgtttgatcg actgggaaaa agtattgaaa ttgcaaagat ttgacctttc tgttatttcg
     6781 attacgattg agctgtttgg aatatcaagt tcttcttcgc cggaatccgt cagcaattct
     6841 ccctgataaa taacatccgg atcgacattc aggaataaaa gccttttcat cccttgcttg
     6901 gccgcatttt ccaatgcatc cttgcggaac agagtatcaa gttcccctat catttttcgg
     6961 ttttttgcct cgtaaatcat gctcaaaggg gaatacatat tcgagttttc cgggcctctg
     7021 ctgaaggcct cataaccaat gatattcccg tcgctcagat ccaccacagg ctgaaaaagc
     7081 gtattgatca gattattttc caccactaat ttgataaagt cctcgttctg gttcatcatg
     7141 tcacctcttc ttagcgaaat attccgaatt tctccgcaga ttgattaatt tccgcaggag
     7201 tgataatata tgttcaaatc cggctgcctc ccgtgcatgt gatcgacggc aaaaacgaaa
     7261 accggcgaat gccgagcggc agcagaatat gattttcacg cttaaattaa cgctatttta
     7321 aatgtttatc aaagcggagg ctccaatgaa agtagtttta gcagcattaa actcaaaata
     7381 tatccatatg tccctcgcac cgtggtacct gaaatcctat tcgggccggg acagcatccg
     7441 ggtcatggag ttcaccgtca atcaggacgc cggtgacatt ctgcgcgata tttaccggga
     7501 aaagcctgat gttctgggct tttcctgtta cattttcaat atccagacgg tggagcaaat
     7561 tctggaagat atcaaaaaag taatgccgga gatcaagatc attctgggcg ggccggaggt
     7621 ttcttttgac ccgcgggatg tcctgacacg gtgcaaagcg gtggattacg tcatctgttc
     7681 cgcgggggaa atccccttta aaaagctgct cgacgagctt gaatccggcc gtgaacccga
     7741 aaagatcgga ggggttgccc tgcgcgccgg gaacgggata ctctgcaatc cgccggacgt
     7801 tcccctgtcc cgaagccttg ccgccatacc ttccccctat accgacgaga tgcttgcgca
     7861 ggccaaaggg aaaattctct attttgaaac ctcgagggga tgcccgttcc actgctccta
     7921 ctgcctgtcc tcagaaagcg gcggcgtacg ttattttccg atggaaagag tccgtcggga
     7981 tcttaaaaca atcgcattgt ccgaagtcag acaggtaaaa ttcgttgacc ggactttcaa
     8041 ctgcaattcg tcccgcgcaa aagaaatttt tcgttatctg attgatttgt cccaaagtct
     8101 gggggaaaac catttcaaaa actatcattt tgaagcggcc gccgacctgt tcgacgatga
     8161 gatgattgag ctgctctctc acgcgccgga gggtttgttc cagttcgaaa tcggcataca
     8221 gagcttcaac gagccgacgc ttgacgcctg cggaagaaaa acagacctgt cggtgtgcgc
     8281 caattatatt tcacgactgc tggaagccgg gaacattcat attcatcttg atctgatcgc
     8341 cggcctgccg ctggaagacc tgcctacctt cgcccattct ttcaacagtg tttacgccct
     8401 tcgcccccac tgtatccagc ttggattcct caagcttctc aagggctccg gcatgcggcg
     8461 ggacgcgcgg cgccattcca ttgattatag tgcccggcct ccctacgaag ttcttcagac
     8521 cgcttcgatg agctttgatg atctaatctt tctcaagagc attgactttt gcgtcgaccg
     8581 gctttataac agcgaaaaat tcagccagag cctgaaattt ctgatcagcc ggagtgagtc
     8641 gcccttccgg ctgttttcgg atttttccgc ttttctcaac ggaaaaccgg aaaacggcat
     8701 ttcactgaaa aatctttacg gtcttctgta ccattttgcg gagcaatcgc tccgcccgga
     8761 tgatctggat gtttttaatg aattcataaa atacgactat ttttcctctg acaactcctg
     8821 caatccgccc cgcgagataa aacggtgtat cgaccctcaa atcaagcagc tttacgccgg
     8881 tcttaagccg ggaggggagc gcgtccactt tgaaagattt gaaattgacc ctctcgacag
     8941 cacaaaaaaa ttcccggaac gaaaggaaac cgtcctgcga tttgactatg gccgccgcaa
     9001 tcgggtgacc gggctttatg gcgttacccc tataagctga cattttgtga caccatttga
     9061 cactactctt atattttata acttttttct cttttgtaaa taactaaaag gaatttattt
     9121 ctaacatttt gataaattat acactctgcc ttttggcagg gcgtgtcgaa cctccgcatt
     9181 tttccgtatt tcggaggtaa ataggtcgct ttcattatcc ccgtctgttg acggggaata
     9241 ttttcatctt ccggtatccg gctggttcag gactccgctt tgagtgactc cagcgtttcc
     9301 cgaatggtgt cgatcaccga ttcgccggtt ttcattttaa cggaaacata cggtttctgc
     9361 cctgcattga ccatcaccct gcccctgagc ctggtcacaa gccctgaaac ggctttgaag
     9421 tcgagttttt ccgggaaaac cagaatcatg ctttcccttt gggaaatttc ccggataccg
     9481 agagaagccg cggtatttcg aatcagcgca acatcgacaa ggcttttgac ctcgacgggc
     9541 ggttcgccgt aacggtcgat catctcgtca atgacatcgg aagcatcctc ttcatcgaga
     9601 atcgaggcga tccttcgata ggtttcgagc ctgagattgg agctttcaat ataattttcc
     9661 gaaatatggg ccgaaatctg cagatcgacc atacattcgc cggcagaagc cggtttttct
     9721 cccttttcct ccaaaacggc ctcgttaagc agtttgagat acatgtcata gcccaccgct
     9781 tccatatggc cgtgctgctg ggaaccgaga atattaccag cccctcggat ttccagatcc
     9841 cggagcgcga tctggaatcc ggaaccgaac tccgtgaatt cgcggattgc cgacag
//
LOCUS       DATALY010000025        11521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_351103,
            whole genome shotgun sequence.
ACCESSION   DATALY010000025 DATALY010000000
VERSION     DATALY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351103"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VHR42_00885"
     CDS             <1..596
                     /locus_tag="VHR42_00885"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX3025777.1"
                     /translation="WGNFFNREAYGAETNLPWRMALDNSGAAYHPCFLYESIWCILGF
                     VLLHFYSKKHRKFNGEIFLMYVAWYGFGRFFIEGLRTDSLMLGNTPIRVSQLLSALAF
                     FAAIITLIVVYKKLWDKRQNDTTGYTPIYSAGINSVEDTDTAETTNETAETETDGEAA
                     EEPSSEPSKAETEPKEAAETDKEEILPENEEKNTEEN"
     gene            597..1451
                     /gene="folD"
                     /locus_tag="VHR42_00890"
     CDS             597..1451
                     /gene="folD"
                     /locus_tag="VHR42_00890"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX3025778.1"
                     /translation="MPAQILDGKAIAAGVKARVAGETEKLKTLGIVPGLAVVIVGNDP
                     ASRVYVNNKRRDCEQVGITSYEYAMPEETTQEELLSLVHKLNEDRTVNGILVQLPLPK
                     HLDEKSVINSINPAKDVDAFHPVNVGRIMIGDFDFLPCTPAGVMEMLDSAGIDPKGRE
                     CVVIGRSNIVGKPMGMLLLHRHGTVTICHSRTKNLREVTQRADILVAAVGKANFVTAD
                     MVKEGAVVIDVGMNKKPDGKLTGDVDYENVCQKASYISPVPGGVGPMTRAMLLVNTIR
                     AAKLQNSL"
     gene            1559..2290
                     /locus_tag="VHR42_00895"
     CDS             1559..2290
                     /locus_tag="VHR42_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3025779.1"
                     /translation="MKIAVIYGSPRNGSTNKAVSLFKERMQQCGNVEFREFFLPKDMP
                     EFCRGCFTCILNDEHKCPHFRFTEPVVSALLEADGFIFSSPVYVMAESAGMKALLDHL
                     AWLFLVHRPKPEMFAKKAVILSTTAGAGTRRCIAMIRESLLYWGVNRIYGYGFILHSA
                     SWKGIEEKRREKTENKIQAKAVKFYREVASGKRRRPYLKQWFMFNAVRMFIKKDKNTS
                     VDRKYWEKNGWLGKNSPFKQPTGQR"
     gene            2426..2737
                     /gene="rplU"
                     /locus_tag="VHR42_00900"
     CDS             2426..2737
                     /gene="rplU"
                     /locus_tag="VHR42_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX3025780.1"
                     /translation="MYAVIETGGKQYRVQEGDTVFVEKLGVEENDAVVFDKVMALGLD
                     DGLKAGAPYVEGAKVTAKVVKNGKEKKVIAFKYKAKKNYRNKKGHRQPYTKVMIEKIE
                     A"
     gene            2774..3034
                     /gene="rpmA"
                     /locus_tag="VHR42_00905"
     CDS             2774..3034
                     /gene="rpmA"
                     /locus_tag="VHR42_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX3025781.1"
                     /translation="MAHKKGVGSTKNGRDSESKRLGVKRSDGQAVLAGNILVRQRGTK
                     IHPGDNVGKGSDDTLFALIDGKVRFERMGRDAKKVSVHAIEQ"
     gene            3179..4438
                     /gene="leuC"
                     /locus_tag="VHR42_00910"
     CDS             3179..4438
                     /gene="leuC"
                     /locus_tag="VHR42_00910"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX3025782.1"
                     /translation="MPMTMSQKILASHAGLSSIQAGQLIEARLDLVLANDITAPVAIQ
                     EFKKTGAKQVFDKSRVALVMDHFTPNKDIKAAAQCMYCREFAGEYEIENFFDVGQMGI
                     EHALLPEKGLVVSGDLVIGADSHTCTYGALGAFSTGVGSTDLAAGMARGVCWFKVPSG
                     VKFVLRGELPRWVSGKDVILHIIGRTGVDGMLYKSMEFTGDGLKSLSMDDRFTIANMA
                     IEAGAKNGIFEADGVAAQYVREHSQKPFTVVKADENAPYDEVYDIDLSSLRPTVAFPH
                     LPENTRTVDNCGTVKIDQVVIGSCTNGRLSDLKTAAELLQNRKIAKGVRCIVIPATQK
                     IYLEAMERGYLKTFIEAGCAVSTPTCGPCLGGHMGVLAKGERAVCTTNRNFVGRMGHS
                     ESEIYLASPAVAAASAVTGYITDPGQL"
     gene            4462..4953
                     /gene="leuD"
                     /locus_tag="VHR42_00915"
     CDS             4462..4953
                     /gene="leuD"
                     /locus_tag="VHR42_00915"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3025783.1"
                     /translation="MKASGRAFLYGDNINTDVIIPARYLNTSDPKELAAHCMEDIDAD
                     FVKKIKSGDILVAGKNFGCGSSREHAPIAIRASGVSCVIASSFARIFYRNAINIGLPI
                     LECKEAAESIENGDEVEIDFDAGIISDKTKKKSYKAAPFPEFIQNIIRSGGLINAVRN
                     GKL"
     gene            4971..6047
                     /gene="leuB"
                     /locus_tag="VHR42_00920"
     CDS             4971..6047
                     /gene="leuB"
                     /locus_tag="VHR42_00920"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX3025784.1"
                     /translation="MEYNIALIKGDGVGPEITNEAVKALNRVGELFGHRFRFHEVLMG
                     GRAIDEKGTPLPDETVETCRKCDSVLLGAVGGPKWDTLPGNLRPEKGLLGIRSALGLF
                     ANLRPARLYPALKNACPLKNEICGGGIDLMIVRELTGGIYFGKRGRKNGENGAEAFDT
                     ELYSENEIRRIGRIAFEIARLRRKKVVSVDKANVLESSRLWRETMHALSRQYPEVEYS
                     DMLVDSAAMQLVKNPARFDVLVTSNLFGDILSDEASMLTGSIGMLPSASLGNGTPSLY
                     EPIHGSAPDIAGKNVVNPVGSILSAAMMLRISLKLEKEADAIENAVARALSDGLRTMD
                     ICGKGGTVMNTEQMGSAVAERITA"
     gene            6073..6771
                     /locus_tag="VHR42_00925"
     CDS             6073..6771
                     /locus_tag="VHR42_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3025785.1"
                     /translation="MEKILLIEDDSALVAGLRYSIEKEGYDITTAGSVREGCEKFFSG
                     NFSLVVLDIGLPDGTGFDVCRTIREKSMIPILFLTACDEEVNVVMGLDMGADEYITKP
                     FRLRELLSRIRALLRRSGTARAADILESGCFRLNTSDCRLFKGKTEITLTPAEYRLVF
                     FLMKNEGRTITRDQLLEKLWDAGGDYVDANTLTVYIRRLREKIEDDPSLPLYISTVRG
                     LGYRWEAAPAQSDS"
     gene            6842..8098
                     /locus_tag="VHR42_00930"
     CDS             6842..8098
                     /locus_tag="VHR42_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3025786.1"
                     /translation="MISKPYVLENAGCRRLFIFLATASFIFLLILLAATASSRRALET
                     GFISRESAVAGKLLNAHPEMDKTEIVRAFTTDTGAKDYQAGSDALKSAGYSIETADRF
                     FAFAGTGYIGGLLFSFLALAALLLLFFAAMYLSLKNIYQRIDHIAASAHCVTRGEYNT
                     KISDTVEGAFSRLSHSFNEMTGSVAAGFEKLAHERNFLKNLLSDISHQLKTPLSALKM
                     YNEIILQEEISPTVRDFIEKSGGQIERMEWLALGLLKTAQIEAGTLEFDMKPENAAAM
                     AADCVDSFAVTSRESGVSLRSGGISSTVLRCDRRWLCEALSNVIKNCIEHTPPGGTVT
                     VTTDETPVTVSFTVSDTGRGIYPDDLPHIFKRFYTGHGQTGGNSAGIGLSLAKSITEQ
                     LGGTLTAKNSLSGGAVFEFTFLKNVI"
     gene            complement(8318..8890)
                     /locus_tag="VHR42_00935"
     CDS             complement(8318..8890)
                     /locus_tag="VHR42_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HEX3025787.1"
                     /translation="MGLKVGFAITGSFCTFEKTFAQLPAFIEAGYEIIPIMSETAYST
                     DTRFGTAQSFVERLEKLTGKSVIHTIKDAEPFGPKALLDILVIAPCTGNTLGKLAGGI
                     TDTSVTMSVKAHLRNQRPVVIAVSSNDALAASARNIGQLINIKNIYFVPMRQDDFVKK
                     PTSMVADMTMILPAVQAALEGKQIQPVYIK"
     gene            complement(8893..9771)
                     /gene="dpsA"
                     /locus_tag="VHR42_00940"
     CDS             complement(8893..9771)
                     /gene="dpsA"
                     /locus_tag="VHR42_00940"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HEX3025788.1"
                     /translation="MLRNKTFAVIGGDMRQVYLAGLLSNDDGYSVLAVGFDRDIVLAP
                     KVMRCKSATEAVQKAGCVILPLPFTVDGITVNAPYARTPLPLEEIWGAAESDTLITGG
                     KLTEKVWDISRRHGFKLVDYYDREELEVLNTIPTAEGAIQIAMEELPITIHNAKCLVM
                     GYGRVSKMLCHDLKALGAQVTACARKYADLAWIKAMGYNAVHITNLPAVIGGFDVVFN
                     TVPAVILTESVLSNLRKDCLVIDLASKPGGVDFETAGHLGLKTVWALSLPGKVAPITS
                     GEIIKDTIINIISEEV"
     gene            complement(10228..10377)
                     /locus_tag="VHR42_00945"
     CDS             complement(10228..10377)
                     /locus_tag="VHR42_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025789.1"
                     /translation="MEYFTVGPPGVLLFTEVRDETGFDAGCETPGLEEMAEETTPADD
                     SAADE"
     gene            10364..10717
                     /locus_tag="VHR42_00950"
     CDS             10364..10717
                     /locus_tag="VHR42_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX3025790.1"
                     /translation="MKYSIDARKAVDKSTGKSFGMLLPECDVPLQSGDTVYSVLKRIC
                     KLKKLALDATTIGEAYVKGIGGAEEFDNGPQSGWIYRLNGVYAASGCSIKLKAGDRVE
                     WIYTTDYGKSEGGTK"
     gene            complement(11203..>11521)
                     /locus_tag="VHR42_00955"
     CDS             complement(11203..>11521)
                     /locus_tag="VHR42_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025791.1"
                     /translation="YNADGNVIRLIASDNKAIRDYIPKIMESPFVTEIVTDTGLKEKI
                     SIGGSKSDPSRVVAWAFTERDGTLFHIVADLSFSFAVGNKDILMNVAKSFGFEKSEAV
                     CGK"
BASE COUNT         2901 a         2929 c         3029 g         2662 t
ORIGIN      
        1 gctggggcaa ctttttcaac cgcgaagcat acggcgccga aaccaatctt ccctggagaa
       61 tggcgcttga caattccggc gccgcatacc atccatgttt cctttatgaa tcgatctggt
      121 gcatcctcgg ctttgtcctg ctgcattttt attcaaagaa acaccgcaaa ttcaacgggg
      181 agatcttcct catgtatgtg gcatggtacg gcttcggccg gttcttcatc gaagggctcc
      241 gcaccgacag tctgatgctg ggcaatactc cgatccgggt gtcgcagctt ctttccgcgc
      301 ttgcgttttt tgcggcgatc ataactttga tcgtggttta taaaaagcta tgggacaaaa
      361 ggcagaacga cacaaccgga tatacgccga tctattccgc cggtataaac agcgtggaag
      421 acacggatac tgccgaaaca acgaatgaga cggcggaaac cgaaacggac ggcgaagcgg
      481 cggaagagcc ttcttccgaa ccctctaaag ccgaaacaga gccaaaagag gctgctgaaa
      541 cggataaaga ggaaattcta cccgaaaatg aagaaaaaaa cacggaggaa aactgaatgc
      601 ccgcacaaat attggacggt aaagccattg ccgccggggt aaaagcgcgc gtcgcaggcg
      661 aaacggaaaa gctgaaaacc cttggcatag tcccggggct tgcggttgtc atcgtcggga
      721 acgaccctgc ttcacgcgtc tatgtcaaca acaaaaggcg cgactgtgaa caggtcggca
      781 ttacttctta cgaatatgcc atgccggagg agaccacgca agaagagctg ctttcgctgg
      841 ttcataagct taacgaagac cgcacggtga acggcatcct tgtccagctg cccctgccga
      901 agcatcttga tgaaaaatcc gtaatcaatt cgatcaatcc ggcaaaggat gtcgacgctt
      961 tccatccggt taacgtcggg cgcatcatga tcggcgattt tgattttctc ccctgcacac
     1021 cggccggggt tatggaaatg ctggactcgg cggggatcga ccccaaagga cgggaatgcg
     1081 tcgttatcgg gcgcagcaat attgtgggaa aacctatggg gatgcttttg ctgcacaggc
     1141 acggaaccgt taccatctgc cattcgcgta cgaagaatct cagagaagtt acccaacgcg
     1201 ccgacattct cgtggctgcc gtcggcaagg cgaactttgt tacggcggac atggtaaagg
     1261 aaggcgcggt ggtaatcgac gtggggatga acaaaaagcc agacggcaaa ctcaccggcg
     1321 atgtagatta tgaaaatgtc tgtcaaaagg cgtcctatat ttctccggtt ccgggcggcg
     1381 tcggccctat gacccgcgcc atgcttctgg tgaatacaat ccgtgccgca aagctgcaga
     1441 acagtctgta agtaacgtaa aatccatgcg gcgctgcata taaaataaat gccgggcggt
     1501 tctattcatt gtgttaatag aaccgcctga tttttaaagg attcaggtgt tgcagtttat
     1561 gaagatagcc gttatttatg gaagcccgag gaatggcagc accaacaaag cggtaagtct
     1621 tttcaaagaa agaatgcagc aatgcggaaa cgtggaattc cgtgaatttt tcctgccaaa
     1681 ggatatgccg gaattctgcc gcggctgctt tacctgtatc ttaaacgacg agcataaatg
     1741 cccgcatttt cggtttaccg agccggttgt cagcgctctg cttgaagccg acgggttcat
     1801 tttttcctca cccgtttatg tcatggcgga atccgccggg atgaaagctc tgcttgacca
     1861 tttggcatgg cttttccttg tgcaccggcc gaaacctgaa atgtttgcga aaaaggctgt
     1921 gattctttcc actactgccg gagcgggtac gcgcagatgt atcgctatga tccgggaaag
     1981 cctgctttac tggggtgtga accggattta tggttacggt tttatactgc attccgcaag
     2041 ctggaaaggc atagaggaaa aacgccgcga aaagacggaa aataaaattc aggcaaaagc
     2101 cgtgaagttt taccgggagg ttgcctccgg caaacggaga aggccgtatt tgaaacagtg
     2161 gtttatgttc aatgccgtaa ggatgtttat taaaaaggat aaaaacactt ctgttgaccg
     2221 gaagtattgg gaaaagaacg gatggctcgg caaaaattcg cctttcaagc aaccgaccgg
     2281 gcaaagatga aatttcaaga attcagaata atatttgaca agctttttga gctgtgttat
     2341 aatgaacttt gccgcttgcc aagggcttga aaggggtaac cctgccccga gcagcgagat
     2401 tgaattcaag caggtgtctt gagtaatgta tgcagttatt gaaacaggcg gtaaacaata
     2461 ccgtgtgcag gaaggcgaca ctgtttttgt cgaaaaactc ggcgttgaag aaaacgacgc
     2521 ggttgttttt gacaaagtaa tggctctggg cctggacgac gggctgaaag ccggagcacc
     2581 ttatgtcgaa ggtgcaaagg tcaccgcgaa ggtcgttaag aacggcaaag agaaaaaggt
     2641 aatcgctttc aagtacaaag ccaaaaagaa ttaccgtaac aaaaagggcc acagacagcc
     2701 ctataccaag gtaatgatcg aaaagatcga agcgtaaggc catttaattt aaaaatttcg
     2761 gaggtgtggc tatatggcac ataagaaggg cgttggctct accaaaaacg gccgcgacag
     2821 cgagtcaaaa cgtctcggcg taaagcgctc cgacggtcag gcagttctcg cgggaaatat
     2881 tcttgttcgt cagcgcggaa cgaaaataca tccgggcgat aatgtcggaa aaggctcgga
     2941 cgatacactt tttgctctga tcgacggaaa agtccgtttt gagcgcatgg gacgcgacgc
     3001 gaaaaaagtc agcgtacacg cgatcgagca gtaatctgat tataaaaggc agttccccga
     3061 agcggtttcg cttcggggaa ctttttttgc cgccgccggc tgacgctttc cttgaaaggt
     3121 cctgtgattc atgttatact aatattgtaa aaaccatttc ccgaaaggat cgaacgccat
     3181 gccgatgacg atgtcgcaga agattcttgc gtcccacgcc ggcctttctt ccattcaggc
     3241 gggacagctt atcgaagcgc gtcttgacct tgttcttgcc aacgacataa ccgctcccgt
     3301 ggcgattcag gaatttaaaa agaccggtgc gaaacaggtc tttgacaaaa gccgcgtggc
     3361 tctagttatg gaccatttta cgccgaacaa ggatataaag gctgcagccc agtgcatgta
     3421 ctgccgcgaa tttgccgggg aatatgaaat cgaaaacttt tttgacgtcg gacaaatggg
     3481 catcgaacac gccctgctgc cggaaaaggg gcttgtggtc agcggcgacc ttgtcatagg
     3541 cgcagattcc cacacttgca catacggcgc gctcggcgcc ttttccacag gggtcggcag
     3601 cacggacctc gccgccggaa tggcacgcgg cgtctgctgg ttcaaggtgc cgtccggcgt
     3661 aaaattcgtc ctgcgcgggg aacttcccag atgggtttcc ggcaaggatg tcatccttca
     3721 cataatcggc cgtacaggtg ttgacggaat gctctataaa tccatggagt ttaccgggga
     3781 cgggctgaaa agcctttcga tggacgaccg ttttacgatt gcaaacatgg cgatcgaggc
     3841 cggtgcgaaa aacgggattt ttgaagcgga tggggtggcc gcccaatatg tccgggaaca
     3901 ttctcaaaag ccctttacag ttgttaaggc agacgaaaac gcgccttatg acgaggtata
     3961 cgatatcgac ctttcctcgc tccgcccgac ggtggctttc ccgcaccttc cggaaaatac
     4021 gcggacggtc gacaactgcg gaacggtaaa aattgaccag gttgtcatcg gctcctgcac
     4081 aaacggccgg ctcagcgacc tgaaaacagc ggcggaactt ctgcaaaacc ggaaaattgc
     4141 aaagggcgta cgctgcattg taattcccgc aacccagaaa atttatctgg aagccatgga
     4201 acggggatat ctgaaaacct ttatcgaagc gggctgcgcg gtttcaaccc cgacgtgcgg
     4261 gccctgtctc ggcggacata tgggcgtgct cgcgaaaggc gaacgcgcgg tctgtaccac
     4321 aaaccgaaac tttgtcggtc ggatgggcca cagcgagtcg gaaatttatc ttgcaagccc
     4381 cgctgtcgcg gccgcttcgg ctgtaacggg atatattacc gacccgggac aactttaaaa
     4441 ttgcgaagga agagctgcac tatgaaagct tccggcagag cgtttctgta cggcgacaac
     4501 atcaacaccg acgtcatcat cccggcaagg tatctgaata catctgaccc aaaggagctt
     4561 gctgctcatt gtatggaaga cattgacgcc gatttcgtca agaagattaa aagcggcgat
     4621 attctggtgg ctggaaagaa ctttggctgc ggttcctcaa gggagcatgc gccgatcgcc
     4681 atccgggcgt ccggtgtgtc ctgcgtcatt gcgtccagct ttgcccggat cttttaccgc
     4741 aacgccatca atatcggcct gccgattctg gaatgcaaag aagcggccga aagtatcgaa
     4801 aacggcgacg aagtggaaat cgatttcgac gcgggcatta tctcggataa aacgaagaag
     4861 aaaagttata aggcagcgcc tttcccggag tttatccaga atattatccg aagcggcggc
     4921 cttataaacg ctgtccgaaa cgggaaactc tgaaaaacgg ggtgacggtc atggaataca
     4981 acattgcgct gataaagggc gacggagtcg gccccgagat tacaaacgaa gccgtaaagg
     5041 cgctcaaccg ggttggcgag cttttcgggc accgtttccg ttttcacgaa gtcttaatgg
     5101 gcggacgggc cattgacgaa aaaggtacgc cgctgccgga cgaaacagtg gaaacctgcc
     5161 ggaaatgcga cagcgtgctg ctcggagccg taggaggccc gaaatgggac acgctgcccg
     5221 gaaacctgcg tcccgaaaag gggctgctcg gcatccgttc cgctttgggg ctttttgcga
     5281 acctgcgccc cgccaggctg taccccgccc tgaagaacgc ctgcccgctg aagaatgaaa
     5341 tctgcggcgg cggaatcgac ctgatgatcg tccgggagct taccggcggc atttatttcg
     5401 gtaagcgggg caggaaaaac ggcgaaaacg gagccgaggc atttgacacc gagctttaca
     5461 gtgaaaatga gatcagacgc atcggacgga ttgccttcga gatcgcccgg ttacggcgga
     5521 aaaaggtcgt cagcgtcgac aaggcaaacg tactggaatc ctcccggctt tggcgtgaga
     5581 ccatgcacgc cctttcccgg cagtacccgg aagtcgaata ttcggacatg ctggttgaca
     5641 gcgcggccat gcagcttgtg aaaaatcctg ctcggtttga cgtgctcgtc acttccaatc
     5701 tgttcggcga catcctctcc gacgaagcaa gcatgctgac cggctccatc gggatgctgc
     5761 cgtccgcttc acttgggaac gggacgccca gcctttacga gccgattcac ggctcagcgc
     5821 cggatatcgc cgggaaaaac gttgtaaacc cggtcggctc gattctttcg gccgccatga
     5881 tgctgcggat ttctttaaaa ctggaaaagg aagcggatgc gatcgaaaac gcggttgccc
     5941 gcgcgctttc agatgggctc cgcacaatgg atatatgcgg caaaggcgga actgttatga
     6001 ataccgaaca aatgggcagc gccgttgccg aaaggataac ggcttaaaac ttttacaggc
     6061 ggtcgataag acgtggaaaa aatacttttg atcgaagacg actccgctct tgtggcgggg
     6121 cttcgctaca gcattgaaaa agagggctat gacatcacaa cagccggaag cgttcgcgaa
     6181 ggctgtgaaa agttcttttc agggaacttt tctctcgtgg tactcgacat cggcctgccg
     6241 gacggaaccg gctttgacgt ttgtcgcacc ataagggaaa aatccatgat cccgattctt
     6301 ttccttaccg cctgcgacga agaagtcaac gtcgtgatgg ggcttgatat gggcgcggac
     6361 gaatatatca ccaagccttt ccggctgcgt gagctgctgt cgcgtatccg cgcgctttta
     6421 agacggagcg gcacggcgcg ggcggccgac attctggagt ccggctgttt caggctcaac
     6481 acgtccgact gccggctttt taaaggcaaa acggaaatta cgctcactcc ggcggaatac
     6541 cggcttgtct tttttctgat gaaaaacgaa ggccgcacca tcacccgcga ccagctgctc
     6601 gaaaagctct gggacgccgg aggggattac gtcgacgcga acacccttac ggtttatatc
     6661 cggaggctgc gcgaaaaaat tgaagacgac ccgtccctgc cgctttacat ttccacggta
     6721 aggggattgg gctaccggtg ggaggccgcc cctgcccaga gtgattcata acactttaac
     6781 atctctttca tcgggaagcc tgttcaaaag cctgggtgaa accaaaagga cggtatataa
     6841 aatgatttct aaaccttatg tacttgaaaa cgccggatgc aggagacttt ttatcttcct
     6901 tgcgacggcg tcctttattt ttttgcttat tctgctggct gcgacggcct cgtcccggcg
     6961 cgcgctggaa accgggttta tatcccgtga aagtgccgtg gccggcaaac ttttgaatgc
     7021 gcatccggaa atggacaaaa ccgaaatcgt ccgtgcgttt acgactgata ccggtgcaaa
     7081 agactatcaa gccgggtccg atgccctaaa aagcgccggt tattcgatcg aaacggccga
     7141 ccgctttttc gcctttgcgg gtaccggtta tataggaggg cttctgttct cttttcttgc
     7201 cctcgccgct cttcttctgc ttttttttgc agcaatgtat ctttctttaa aaaacattta
     7261 tcaaagaatc gaccatatcg cggcgtcggc ccattgcgtt acccgcggcg aatataatac
     7321 gaagatttcc gataccgtcg agggcgcttt ttcgcggcta tcccattcgt ttaacgaaat
     7381 gaccgggtct gttgcggccg gattcgaaaa gctggcgcat gagcggaatt ttctcaaaaa
     7441 cctgctctcc gatatttccc atcagttaaa aacgccgctt tctgccctga aaatgtacaa
     7501 tgagattatc ctgcaggaag aaatcagccc tacggtgcgg gattttattg aaaaaagcgg
     7561 cgggcagatc gagaggatgg aatggcttgc cctcggcctc ctgaaaaccg cgcagatcga
     7621 agctggtacc ctggagtttg acatgaaacc ggagaatgcg gcggcgatgg cggcggactg
     7681 cgtcgatagt tttgccgtta cgtcccgtga aagcggcgtg agccttcgtt cgggcgggat
     7741 atcctccact gttctccggt gcgaccggcg ctggctttgc gaggccttga gcaatgtgat
     7801 aaaaaactgt atcgaacaca ccccgcccgg cggaaccgtt accgttacga ccgatgagac
     7861 ccctgtaacc gtttccttca cggttagcga caccggccgc gggatttatc ccgacgatct
     7921 gccccatatt ttcaagcggt tttatacggg ccatgggcag actggcggca acagcgctgg
     7981 aatcggcctg agccttgcca agtcgattac cgaacagctc ggagggaccc tgaccgcaaa
     8041 gaacagcctg tccggcggtg ccgttttcga gtttacattt ctgaaaaatg tcatataaat
     8101 gttcgtcagg gtccgaaaaa aagcggcgtg ctataaaaaa taaggacagc cataagctgc
     8161 cctacttgaa aacaaaagac tactaaaata ttgcgcaaaa agtttttatt tagaaagccg
     8221 acggcaactt tcaccgcgcg ggatagacca ggccaaagac atgccaagcg gtcgccgaag
     8281 gcggcaaaac ggcgaaggtg atgctgactg tttaatccta tttaatatag accggctgta
     8341 tctgcttccc ttcaagcgct gcctgcacag ccggcagtat catggtcata tccgccacca
     8401 tggaggtcgg ctttttcaca aaatcgtcct gcctcatcgg gacaaaataa atattcttga
     8461 tattgataag ctggccgatg ttcctcgcgg aggctgccag cgcatcgttt gaggaaaccg
     8521 cgatcacgac gggcctttgg ttgcgaagat gcgcttttac cgacattgtg acggacgtgt
     8581 cggtgatacc cccggcaagc ttgccgagcg tgtttccggt gcagggcgca attacgagaa
     8641 tatccagcag ggctttcggg ccgaaaggtt cggcgtcttt tatggtgtgt atcacgcttt
     8701 tcccggtcag cttttcaagc ctttccacaa agctctgcgc tgtaccgaat cgtgtgtcgg
     8761 tggaataggc tgtttccgac ataatcggaa tgatttcata gcccgcttca atgaaagccg
     8821 gaagctgcgc aaatgttttt tcaaacgtac agaaagaacc ggttatggcg aaacctactt
     8881 tcaaacccat ctttacacct cctccgaaat aatgttgata atcgtgtcct tgattatctc
     8941 tccggaggtg atcggcgcta cttttccggg cagcgacaac gcccatacgg ttttcagccc
     9001 cagatgtccg gctgtttcaa aatccacgcc gccgggtttt gaggcaaggt cgatcacaag
     9061 acagtctttt ctcaggttgg aaagcaccga ttcggtgaga ataacagcgg gaaccgtgtt
     9121 gaataccaca tcaaatccgc cgatgaccgc gggaaggttt gtgatatgta cggcgttgta
     9181 tcccattgcc tttatccagg ccagatcggc gtattttctg gcacatgccg taacctgagc
     9241 gccgagtgct ttcaggtcat gacaaagcat tttggaaacc cgtccatatc ccatgacaag
     9301 gcattttgcg ttgtgtatcg taatcggcag ttcttccatt gcaatctgga tagcgccctc
     9361 cgcagtcgga atcgtgttta aaacttcgag ttcttccctg tcgtaataat ccacaagctt
     9421 aaagccgtgc cgtcgagaaa tatcccagac tttttccgtc agttttccgc ccgttataag
     9481 ggtatcgctt tccgcggctc cccagatttc ttcaagcgga agcggagtcc gcgcatacgg
     9541 cgcgtttacc gttatcccat cgacggtaaa cggcaggggc aggataacgc atccggcctt
     9601 ttgtactgcc tctgtggcgg atttacagcg cattaccttc ggagcaagca caatatccct
     9661 gtcaaacccc accgccagca cgctgtaacc gtcgtcattg gaaagcagcc cggccagata
     9721 cacctggcgc atgtctcccc ctatgaccgc aaaggtttta tttcgaagca tcctaataac
     9781 ctccatacgc tgctgcgtgt aaatatcaaa tgaactgtgt ggaaacgaag atcgaataga
     9841 gaatgcacaa aataaagaga cacctataag tacgaacaat attcgcccgc atttaaatta
     9901 ccggacgtac aatgtgcgcc cctgcaagat gaatttgtgc aatgttttcc ctctatcatc
     9961 gtatgaagat tcgcaccggt aagtgaagaa caaacccgcc tgctgcaata tttttattga
    10021 caggacggct ttatggaggt aaattattta tacgggcagc tcagccccgg caaatcaaaa
    10081 gcaaagcagg aacttataca ttatgaaaaa gctcagcatg attataccat tagttatagt
    10141 acttgtgctc tgcggatgca gcctgcgccc aaaaccggcg tccccttcat ccgaaagcac
    10201 aacagcggtt acctctgtcg cttcatctta ttcatccgca gccgaatcgt cggccggtgt
    10261 ggtttcttcc gccatttctt ccaaacccgg cgtttcgcag ccggcgtcaa agcctgtttc
    10321 atcccgcact tccgtaaaaa gcagcacgcc gggcgggccg actgtgaaat attccatcga
    10381 tgctcgaaag gcggttgaca aaagtacggg aaagagcttt ggaatgcttt tgcccgagtg
    10441 cgacgttcct ctgcagagcg gcgatacggt ttatagcgtt ttaaagagaa tctgcaagct
    10501 caaaaaactc gcgcttgacg cgaccaccat cggggaagcc tatgtaaagg gcataggcgg
    10561 cgccgaggaa tttgacaacg gcccgcagag cggctggatt taccgcctca acggcgtgta
    10621 cgcagcatcg ggatgctcta taaaattaaa agccggtgac cgggtcgaat ggatttatac
    10681 cacggattac gggaaatcag aaggcgggac aaagtaatat tttttcgtcc tgctattgga
    10741 aatttctcca taatattcta taatagacgc gtggaagtta ccataaggcg cacgaatgtg
    10801 ggcctttaaa taaaaaagcc gcgacaggcc ggtgcgtaac agcacaccgt ccctgcatac
    10861 aggtgaagag accacccgca cacaactgtt tacacagcat cgcgacgacc tttattataa
    10921 cccatttctt tccctttttc aaccagaaaa aaatcagaag ttttgggttt gccaagcagg
    10981 ttttccggct tcgggcagcg ttgtggttct gttacagttc aaggctatct ttgaattgat
    11041 ggcgcgtgca ggtgaacccg tttagggctg aatctgtgcg cgttttttta ttgcccgtcc
    11101 tcttacctcc gggctggtag ctccccgaaa atgggtttgc gcggggcatt atgtctcaca
    11161 ataaacgccc cgtgccgccc gcctttttcg gggagctgca acctattttc cgcaaactgc
    11221 ttcgcttttt tcaaacccga aactttttgc aacgttcatt aatatatcct tgtttcccac
    11281 cgcaaagctg aaggacaggt cggccacgat gtgaaacaag gtgccgtccc gttcagtaaa
    11341 agcccatgcg acgacgcggc tcgggtcgct ttttgagccg cctatactga tcttttcctt
    11401 taagcccgtg tctgtgacga tttccgtcac aaacgggctt tccattattt tggggatata
    11461 gtcccggatc gccttgttat ccgaagcgat cagcctaata acgtttccat ccgcgttata
    11521 a
//
LOCUS       DATALY010000026        12813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_351356,
            whole genome shotgun sequence.
ACCESSION   DATALY010000026 DATALY010000000
VERSION     DATALY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351356"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VHR42_00960"
     CDS             complement(<1..176)
                     /locus_tag="VHR42_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3025792.1"
                     /translation="MDWITGVQKAIDYVEDHITENIDYEEVAKQAYSSSFHFQRVFSI
                     LCGFTLGEYIRSRR"
     gene            complement(304..696)
                     /locus_tag="VHR42_00965"
     CDS             complement(304..696)
                     /locus_tag="VHR42_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HEX3025793.1"
                     /translation="MGKFDLHALIGDVRFASWNRICALIDKLYDMETIWSDGGKKWDV
                     EYKFRRGGKTLCGLYAREDCFGFMIIFGKDEREKVEAIRDQLSITTLTAYDSATVYHD
                     GKWVMFDENVPTEDAKILLAIKRKPNRK"
     gene            complement(683..1153)
                     /locus_tag="VHR42_00970"
     CDS             complement(683..1153)
                     /locus_tag="VHR42_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3025794.1"
                     /translation="MQYKCTLLAVKNVEISKKFYGELFDQTVTLDLGWNVTFSGGFAI
                     QQNFGWLCDIPENAIVSKSNNMELYFEVDNFDEFLNKLESYPDINYVNRPKTHEWKQR
                     VVRIYDPDYHIIEIGESMAVIARRYLAEGYSIAETAEIIQHPVQFVEAVKNGEI"
     gene            1579..3087
                     /locus_tag="VHR42_00975"
     CDS             1579..3087
                     /locus_tag="VHR42_00975"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX3025795.1"
                     /translation="MAHYFSEPSRTFNEYLLLPGYSSKECVPYNVSLQTPLVKFNKGE
                     ESAITMNIPMVSAIMQSVSDDKMAVALAMQGGISFIFGSQSVENQAAMVKRAKQHKAG
                     FVYSDSNLKPDQTLSDVLELKASTGHSTMAVTDDGTSNGKLLGVVTGRDYRVTRMSGD
                     TKVREFMTPYESLIFAREGISLKEANDIIWDNKLNSLPIVDTEDHLAYFVFRKDYNAD
                     KEHPDSLLDASKRYVVGAGINTKDYEQRVPALVEAGADVLCIDSSEGFTEWQKMTIDF
                     IRQRYGDTVKVGAGNVVDREGFRYLADCGADFIKIGIGGGSICITREQKGIGRGQATA
                     VIDVAKARDEYFEETGVYIPICSDGGIVYDHHITLALAMGADFVMLGRYFARFDESPT
                     NKLNINGNYVKEYWGEGSSRARNWQRYDMGGASKLSFEEGVDSYVPYAGNLEDNVKAT
                     LSKVKSTMCNCGVTSISELQKNAKLTVISSTSIVEGGAHDVILKDTNVITVK"
     gene            complement(3305..3994)
                     /locus_tag="VHR42_00980"
     CDS             complement(3305..3994)
                     /locus_tag="VHR42_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3025796.1"
                     /translation="MRQEIVLISASPRVTADAASAQFVEMASKRLKDENTETTIIDVR
                     RSFSRENTREDFEVITWANAIVIAFPLYFFCLPGMLTRFLEDYRDYLLETGNRNLHPK
                     IYAIVNCGSPEAGMNEEAVRVVRSFSLQIKAEFRFGIMIGGGMLIGTKDAPFMKKNLS
                     SLNDAFSSLKQDILNDGRSFLVNHLINPNFPRKLYLYLGNRRLIHAARKNGLNKKDLH
                     CRPYQSDARNK"
     gene            complement(3991..4551)
                     /locus_tag="VHR42_00985"
     CDS             complement(3991..4551)
                     /locus_tag="VHR42_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3025797.1"
                     /translation="MAALVLSDQDDKSELRLHLEELISNCLSERGLQTSRIRVGRNDL
                     GFCMGCFGCWVRKPGECVIGDAMGEINRQCMNSDVVVYLSPVVFGQFSANLKNVIDRW
                     LPNMLPFFETNPDGSTIHPSRYASNPCQFMIGYGQNVTEEDAQLFCDITQKHRNKTEA
                     LVYRGDDTQIIGALSRMKLERVSGQL"
     gene            complement(4606..6135)
                     /locus_tag="VHR42_00990"
     CDS             complement(4606..6135)
                     /locus_tag="VHR42_00990"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HEX3025798.1"
                     /translation="MQRIIEFIKRRAALLTLAAFAATVLPMMIITIYDRPSGDDLGYG
                     RSVYAALRSGGSLLDILNAAVSMVVWTYHTWQGTYSALFLMSLQPGVFGARFYAIGSL
                     LVMGSLIFATFRFSNTLFCVLLRQPRRSALIFSAAMLFCSMQYVPSVVEAFYWFNGAV
                     YYTFYYALMLLYFDALLLLIHGSDKRPWPRLLLALALALILGGGNFVAALVTALGGTA
                     LGGYCLLRRRHAFFRVILPHIILLTGFLISVRAPGNAFRQAKHHKMGVLSAIQFSFAK
                     AAVDIRYWSTFATIALFILLVPILCRAAKGCLCQFRMPGLAMLCAFLLFAAQNTPAYY
                     AMSHAGPGRLRDAVYYSYFWLVAFCLFYCCGWLVRQDFFRKFRSSHRDGTDLFWRRSK
                     KIILSLAAFVFLFSTLKPSAFVRSAGYQCARDLINGTALSYSRQMDARLTQLENPRIN
                     DVRVPGLTAKPEVLVDCDISSNPIDTINRQFGLYYHKHSVASYPAKQAFDFRVSSHKH
                     K"
     gene            6494..7261
                     /locus_tag="VHR42_00995"
     CDS             6494..7261
                     /locus_tag="VHR42_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX3025799.1"
                     /translation="MSEASANQIIRISKLCAQVILENGGETYRAEETVQRICRAFGFD
                     EADVIAIPTGVFITLCGEGNHVSTAIKRIKKRSFNLTAIEEVNNISRMLIGGGITATD
                     ALEHLNRLNSPQKEGRLLSVFAAGMSSGCFTLLFRGSLFDFAVATMCGMLVQLVASSI
                     NIIDMFNFAISILGGIMIAVGSVLSVKIFGTGDLEKIITGAMMPLLPGIPMTNAIRDA
                     MRGDLVSGVTRATEALLVAVALAFGVGVVLKLYYIFL"
     gene            7289..7492
                     /locus_tag="VHR42_01000"
     CDS             7289..7492
                     /locus_tag="VHR42_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX3025800.1"
                     /translation="MNVLRDFLICVPATAFFAILFKVPRRAILISAVLGALGYAVYDL
                     ISPVTHSTIGGYFFRNAVHGGSE"
     gene            7476..7751
                     /locus_tag="VHR42_01005"
     CDS             7476..7751
                     /locus_tag="VHR42_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX3025801.1"
                     /translation="MAVLSEILARVMKMPATIFMIPAIIPLVPGVGLYQTMMFLVQQQ
                     DNQAAKTGTTTLLSIVAMAMAMVFTAILTQFISAIAIMISHKKNPVN"
     gene            complement(7809..8993)
                     /locus_tag="VHR42_01010"
     CDS             complement(7809..8993)
                     /locus_tag="VHR42_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017688603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3025802.1"
                     /translation="MDYVFSNRTNGMKPSAIREIVKYFEGTKCIQFADGNPSAESFPI
                     DAIHKISEHIISDHAAEVFQYGVSEGYKPLRETIKKRMEKCNCVRDFDEIVITSGSQQ
                     AIDLSSRTFLNEGDTVICDNPSYMGALNNFQSYNINLVGIEMEKDGMNIAMLEKSLKE
                     EKNVKLIYVVPTFQNPTGISISLEKRKAIYELAKKYGVIIIEDNPYGDLRYKGQDIPP
                     IKSLDTEGIVIYCGSFSKVLCAGIRIGYALAPASIIDKIVIGKQVDDVHTGSFFQVLA
                     EHYMNQYDFDAHIDSIRELYRHKLSIMTSCLNEKMKECVYQEPEGGLFLWCDLPKWVN
                     SMAYCKQAGENGVAVVPGLTFTIDENPNCNAIRLNFSTPSDEQIEKGITILADTLNSF
                     KK"
     gene            complement(9702..10547)
                     /locus_tag="VHR42_01015"
     CDS             complement(9702..10547)
                     /locus_tag="VHR42_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3025803.1"
                     /translation="MVRVLGFGDNVVDKYEHIKTMYPGGNCVNFAVYAKQNGVESSAY
                     MGIFGTDSEAEHVIHTLGELNIETFKCRQIPGENGCAKVTLENGNRVFIGSNKGGVRK
                     TNPFILDRFDLEYIKTFDLVHTGNNSYTESELSKIRQAGVEVSFDFSHGSTDDYFKKV
                     APDVDYAFLSCSDQTEEEIRVILKKISDYGCKIVVATMGPRGCMAFNGSRYFFEPAVD
                     AEVIDTMGAGDSLATSFMVHYVRGKKQGTDQNTLIPHCLVEGSKFAIKTCMVPGAFDH
                     GARYE"
     gene            complement(10595..11530)
                     /locus_tag="VHR42_01020"
     CDS             complement(10595..11530)
                     /locus_tag="VHR42_01020"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025804.1"
                     /translation="MNEVLKRLCKSYGPPANTKAVAAIIEKEISPFVDEICIDKLGNL
                     IAHKKGLGKKVWIGAAMDQPCMIVTYAPDNGRLKFSVEGILDLRPLSESEVVFEDGSV
                     GTLLPEHESAGGESRDYYIRLSDGGRAGIGDVAVLKGSYSENGALLSSALHQRAGCSC
                     LIDVIKSIPAEAPGNFCFAFTSHSVAEARGAKTASFDFRPDIAVSIGTSPADHRIHPG
                     FGPVLHMRDPASVTHPDLVAAFENAASRIHIQLQREVASHGFSLISGVVKNGFNLKTA
                     SVCLPVAGYQTPDEAADSGDLKDLSALIIGAISLF"
     gene            complement(11527..12513)
                     /locus_tag="VHR42_01025"
     CDS             complement(11527..12513)
                     /locus_tag="VHR42_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEX3025805.1"
                     /translation="MLLKDLASAWGVTGQEGEVRAILKKEIGPYVDEIRRDGLNSMIA
                     VKNGRADLPKVMICAHMDEVGFLITEIHRDGTLGFESLGRIDLKVDASKTIYIGKNRI
                     HGVIRTGRSYRESYIDIGARSSEEAGKHVRVGDGAIFTTRFEDFGDGRIKSKALDDRT
                     GCGVLAETLKNHYEMPVYGVFSVQEEIGERGAFAAAGLVRPDIGIVLEGTVCSNVSVV
                     EPYRHSTSLGEGPAISIADRTSHFDLELSRAACKLAEKNGVPFQRRRITGGGNDGGAI
                     HTAGSGARCITISVPCRYIHSPICETSMEDFRNTIRLVDLFLKEIESGRIGA"
     gene            complement(12504..>12813)
                     /locus_tag="VHR42_01030"
     CDS             complement(12504..>12813)
                     /locus_tag="VHR42_01030"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025806.1"
                     /translation="EGPAIFTGPTSDPELTGALIKFAESSSINYQLRAAAEPEESEAW
                     PLQVACGGIPLAVISIPVKYRYSSVEEANLGDLETAAELLSGYLTILEKADWGKLLCC
                     "
BASE COUNT         3463 a         3096 c         2927 g         3327 t
ORIGIN      
        1 aaacgccttg agcggatata ctcgccaagg gtgaagccgc acagaatact gaacacccgc
       61 tgaaaatgaa agctggacga ataggcctgc ttcgccactt cttcataatc aatgttttca
      121 gtgatatggt cttccacata atcgattgcc ttctgtacgc ctgtgatcca gtccatcact
      181 ccgtctcctt tctgtatgcg cctattatag aggaaactgc atttgacttc gtgacttttt
      241 gtgcggagtg gtgttgcaca atatttttct ccgtccttgc tatttgaaat ggcacaataa
      301 ctgttatttt ctattcggct ttcttttaat tgcaagcaga attttcgcat cttcagtcgg
      361 tacattttcg tcaaacatca cccacttgcc atcatgatac acagtcgccg aatcatatgc
      421 cgtaagagta gtaattgata actgatcccg tatggcttca accttctcac gctcgtcttt
      481 tccgaagata atcataaatc caaagcaatc ctctcttgcg tatagcccac acagcgtttt
      541 gccgcctcgt ctgaatttat attcaacatc ccacttcttg ccgccgtcgc tccatattgt
      601 ttccatatca taaagcttgt ctataagcgc acaaattctg ttccaagaag caaatcgcac
      661 atcacctata agcgcatgca aatcaaattt ccccattttt aaccgcctcc acaaactgca
      721 ccggatgctg gataatttca gccgtctctg cgatcgaata tccctctgcc aaataccgcc
      781 gggcaatcac cgccatagac tcgccgattt caatgatatg ataatcgggg tcataaatcc
      841 gcacaacacg ctgtttccat tcgtgcgttt tcggcctgtt cacatagttg atatcaggat
      901 agctttcgag tttatttaag aattcgtcaa agttgtcaac ttcaaaataa agctccatat
      961 tatttgactt tgagacaatt gcgttttccg gaatatcgca aagccacccg aaattctgct
     1021 gaatcgcaaa gccgcccgaa aaggtaacgt tccagccaag gtcgagcgta accgtctggt
     1081 cgaacagttc gccgtaaaat ttctttgata tttcaacatt cttcaccgca agcaacgtac
     1141 acttatattg catatcaatc acgcttcctg ttcttttttt ccgcagcatg gaatgacatt
     1201 ctggccgata ccgttaaaac cattcgcacc attgataaaa agcatttcct tatgattggc
     1261 ggtgtcaact ataatgcaat tgatccactc agcacattaa agctgccaaa agataaacgc
     1321 ctgattgtta ctgtacacta ttaacacaac aaagcgctgt caatcttgtt atgcatctta
     1381 attggttcct cgggtctgcc atttccacaa aatggatcaa agatcattaa attctttggt
     1441 gtttctcgtt catttatcga tttggcctga tatggataga ttcgtaagct gtttttaatt
     1501 tacttgttaa taaatatatg gtactattta attgatatat ttattgattt ttaattaaaa
     1561 tataaaggag atattacatt ggcacactac ttttctgagc cgtcaagaac ctttaacgag
     1621 taccttcttt taccaggtta ttcctccaag gaatgtgttc cttataatgt ttcgcttcag
     1681 acacctttgg tgaagtttaa caagggtgaa gagtccgcta tcacaatgaa catccctatg
     1741 gtttccgcta tcatgcaatc ggtatccgac gacaaaatgg cggttgcctt ggcaatgcag
     1801 ggtggaatct cctttatatt tggttcacaa tcggttgaaa atcaggcggc aatggtaaag
     1861 cgtgcgaaac agcataaggc cggctttgtt tacagcgatt caaacttgaa gcctgatcaa
     1921 accttatcgg atgtgctcga gctaaaagcc tcaacagggc actcaacaat ggctgtaacc
     1981 gatgatggta catcaaacgg caaactgttg ggcgtggtta cgggacgaga ctatcgggtt
     2041 acaagaatga gcggagacac aaaagtaaga gagtttatga caccgtatga gtcccttatt
     2101 tttgcaagag agggaatctc gctcaaagaa gcaaacgaca tcatttggga caacaagcta
     2161 aactcactgc cgattgttga taccgaagat caccttgcct actttgtttt cagaaaggat
     2221 tataatgccg ataaggagca tccggactct cttcttgacg cttcaaaaag atatgtcgtc
     2281 ggagccggta ttaacacaaa agactatgaa caaagggttc cggcactggt tgaggcagga
     2341 gcagatgtgc tttgtatcga ctcctcagag ggttttaccg agtggcagaa gatgaccatc
     2401 gattttatca gacaaagata cggcgatact gttaaggtgg gtgccggaaa tgttgtggac
     2461 agagagggct ttcgatattt agccgactgc ggtgctgatt ttattaagat aggaatcggc
     2521 ggcggttcaa tttgtattac ccgcgagcag aagggtatcg gaagaggaca agctactgct
     2581 gtgattgatg ttgctaaggc aagagatgaa tattttgaag aaacaggagt ttatataccg
     2641 atctgctcgg atggcggtat cgtttatgat catcatatca ctctcgctct tgcaatgggc
     2701 gctgattttg tgatgctggg aagatacttt gcacgctttg atgaaagccc tacaaataag
     2761 cttaatataa atggaaacta tgttaaagaa tattggggtg agggatcaag ccgcgcccgt
     2821 aattggcaga gatacgatat gggtggcgca agtaagctgt cgtttgaaga gggcgtagac
     2881 tcctacgtcc cttatgcagg aaatcttgag gataacgtaa aagcaacact cagcaaggta
     2941 aaatctacta tgtgcaactg tggcgtcacc tcaataagcg agctgcagaa gaatgcgaag
     3001 ctcactgtaa tatcctctac aagtattgtc gagggcggcg ctcacgacgt catattaaag
     3061 gatacaaacg ttattaccgt aaaatagaac cacgtttatt tgtaaaatca ttatcgttta
     3121 tgatcctaat tggaaccatc tcggaaaacg tgaaaaattg cccgacaaaa ccgccttgac
     3181 aggcggtttt tcctttttta tatcgttctt acattttata gcataaaaaa acagccttgc
     3241 cccatccgtg tattgcaccc aagggaacgg aaatcatagc ccccccttat tccccacgtt
     3301 tcgtttattt gttacgggcg tcggattgat agggtctaca gtgaaggtct tttttattca
     3361 gaccgttttt tcgcgcagca tggatcaatc tcctgtttcc cagatacaga tataattttc
     3421 tcggaaaatt aggattgatc aaatgattca cgaggaagga tctgccgtcg ttcaggatat
     3481 cctgtttcag cgaagaaaag gcgtcgttga gacttgaaag attttttttc ataaatggag
     3541 cgtctttcgt gccgattaac ataccgccgc cgatcatgat tccaaaacgg aactccgctt
     3601 tgatctgaag gctaaaactt cggacgaccc gaaccgcttc ctcattcatg cccgcttccg
     3661 gagagccgca gttgacgatc gcgtagattt ttggatgcag gttcctattg ccggtttcca
     3721 acaaataatc gcggtaatcc tctaaaaatc gcgtcagcat ccctggcaga cagaaaaaat
     3781 agagagggaa ggcaattacg attgcatttg cccatgttat aacttcaaag tcctcacggg
     3841 tattttcccg ggaaaaactc cggcgcacat caatgatcgt tgtttctgtg ttttcgtctt
     3901 ttaaccgttt ggaagccatc tcaacaaatt gagcggatgc agcatctgcg gtgaccctcg
     3961 ggcttgcact gatcagaaca atctcttgtc tcataactgc ccactcaccc tttcgagttt
     4021 catccgactg agagcgccga tgatttgcgt atcgtctccc cggtagacca gagcctctgt
     4081 cttgtttcgg tgtttttgtg taatgtcaca aaacaactgc gcatcctcct cggtaacatt
     4141 ttgaccatac ccgatcataa actggcacgg attggaggcg tatctcgacg gatggatcgt
     4201 ggatccgtcg ggatttgttt caaaaaaagg caacatattg ggcagccaac ggtcgattac
     4261 gtttttaaga ttcgcactga attgaccgaa aacgacggga ctcagataca caacaacatc
     4321 gctgttcata cattgccggt taatttcgcc cattgcgtcc ccgatgacac actcgccggg
     4381 tttccggacc cagcatccaa agcagcccat gcagaagccg agatcgtttc ttccgactct
     4441 gatccggctt gtttgaagtc ccctctccga caggcagttg ctgatgagct cctcaaggtg
     4501 aagacgcagt tctgacttgt catcctgatc cgataaaact aacgcagcca tgatcaaatc
     4561 cactccttaa tcggcggcac atagcagaat tttcccaaag cccggctact tatgcttgtg
     4621 ggaagaaact cgaaaatcaa aagcctgttt ggctggataa gaggccactg aatgcttgtg
     4681 ataatacagc ccgaactgcc ggttgattgt gtcgatcggg ttgctgctga tatcacagtc
     4741 aacaagcacc tcaggcttcg cggtgaggcc gggtacccta acatcgttaa tgcggggatt
     4801 ctccagctgt gtcagccgcg cgtccatctg ccggctgtaa gacagggcgg ttccgtttat
     4861 aaggtcgcgg gcgcactgat aaccagcgct tctcacaaac gcggatggtt ttaacgtaga
     4921 aaagagaaaa acgaaagccg ccagagacag gataatcttt ttggaacgac gccagaacag
     4981 atccgtcccg tcacgatgag aactgcggaa ttttcgaaaa aagtcctgac ggacgagcca
     5041 tccgcagcaa taaaacaggc agaaggcaac caaccagaaa taagagtaat agaccgcatc
     5101 gcgcagcctc ccgggccccg cgtggctcat cgcatagtat gcgggagtat tttgagcggc
     5161 aaacagaagg aaagcgcaca gcattgcaag gcccggcata cgaaattgac agagacagcc
     5221 cttcgctgca cggcaaagga taggcacaag cagaataaac agtgcgatgg tggcaaaggt
     5281 tgaccagtat ctaatatcga cagcagcctt tgcgaaagaa aactgaattg ccgaaagaac
     5341 acccatttta tggtgcttcg cctgccggaa agcgttaccc ggcgcacgga cgctgattaa
     5401 aaaacctgtc agaaggataa tgtgggggag aattacacgg aaaaaagcgt gccggcgcct
     5461 gagcagacaa taacctccga gcgctgtacc gccaagcgcc gtcacgagag ctgcgacaaa
     5521 attaccgccg ccaagaatca gggcaagtgc gagagcaagc agcaggcggg gccagggccg
     5581 cttgtcgctg ccgtgaatca aaagcaggag ggcgtcaaag tacaaaagca tcagagcata
     5641 atagaaggtg tagtaaaccg ctccattaaa ccagtagaac gcttccacga cggaaggaac
     5701 atattgcatg gaacaaaaca gcatagcggc ggaaaagata agcgcggacc ttcgaggctg
     5761 cctgagcaag acacaaaata gcgtattgga aaaccgaaag gtcgcaaaaa tcaggcttcc
     5821 cataaccagc agcgacccta tggcgtaaaa tcgcgcgccg aagaccccag gctgaaggct
     5881 cattaaaaac agggcggaat aagttccctg ccatgtgtga taggtccata cgaccatgct
     5941 cacggcggca ttcaggatat caagcaggga gccgcctgat ctcagagcgg catatacgct
     6001 ccttccgtaa ccaagatcgt cgccggacgg ccggtcatag atggtaatga tcatcatggg
     6061 cagcacagtc gccgcaaaag cggcaagggt cagcaaggct gcccggcgtt ttataaattc
     6121 aatgattctt tgcacgagtt ttcctccaaa aatatgtctg gaaggccgtc agaacgataa
     6181 ttggaaaaac ggcatcctta tttgaaccgg tttttagttt aagcagacag atgccgcata
     6241 gacggcaaca tctttatagc acagacatgg aagcttcccc ttattttaac gtttgtcaca
     6301 tagaattgtc aatgcgtgga agaaaaaaca aataaatgtt tttctccggg tggtaacgga
     6361 gaagtattct aagttctgga taacctaaga acaagcccgt tgactgcgcg gactcttgtt
     6421 ttcaccggga attcaagcta taatggaacc gttccatata gaaagaaaac caattggata
     6481 cggagataat aaaatgtcgg aagccagcgc aaatcaaatt atccgaatct caaaattatg
     6541 cgcccaggtt attctagaaa acggcggcga aacctatcgc gcggaagaaa ccgttcagag
     6601 aatctgccgg gctttcggat ttgacgaagc cgatgtgatt gcaataccaa caggtgtttt
     6661 tataacactt tgcggggagg gcaatcatgt cagcacggct atcaaacgaa tcaaaaagcg
     6721 ttcgtttaac ctgaccgcca ttgaagaagt caataatatt tcaagaatgc tgatcggcgg
     6781 tggaataaca gccactgacg cattggaaca tctgaacaga ctgaattctc cacaaaaaga
     6841 aggcagacta ctctcggttt tcgcggccgg aatgtcgtcg ggctgcttta cgcttttatt
     6901 ccgcggcagc ctgtttgatt ttgccgtggc gacgatgtgc ggcatgttgg ttcagctcgt
     6961 agcgagctcg attaatatca ttgatatgtt taactttgcg ataagcattc ttgggggcat
     7021 catgattgcc gtcggctcgg ttttgtcggt caagatcttc ggaacaggcg atctggagaa
     7081 aatcataacg ggtgcgatga tgccgctcct accgggcatt ccaatgacaa acgccattcg
     7141 ggacgccatg cgcggagatt tggtctcggg tgttacaagg gctaccgagg cgctgctggt
     7201 tgcggttgct cttgcctttg gcgtcggcgt tgttttaaag ctttattata tttttctata
     7261 atagcctgtt cgggagggaa tctataattt gaatgtcttg cgagatttct tgatctgcgt
     7321 acccgccact gctttttttg ccatcctgtt taaagtaccc cgccgtgcca tattgatcag
     7381 cgcggtatta ggcgctttgg gctacgccgt ctatgatttg atttctccgg taacacattc
     7441 cacgatcgga gggtattttt ttcggaacgc tgttcatggc ggttctgagt gaaattctgg
     7501 cgcgcgtgat gaagatgccg gctacaattt ttatgattcc ggcaatcatt ccccttgtgc
     7561 cgggcgtggg gttatatcaa accatgatgt tcctggttca gcagcaggat aatcaggcgg
     7621 caaaaacggg cacgacaacg ctgctttcca tcgtcgcgat ggcaatggcg atggttttca
     7681 ccgcaatcct gacccaattt atctccgcta tcgccataat gatcagccat aagaaaaatc
     7741 ctgtcaatta aaaatgtgac aaatagaaac gcccggcttt cgccgggcgt ttctattatg
     7801 ttttcttctt actttttaaa gctgttcaga gtgtcggcca gtatagttat acctttttcg
     7861 atctgctcat ccgacggagt cgagaaattc aggcggatgg cgttacagtt ggggttttca
     7921 tcgatggtaa aagtaagccc aggaacgacc gccacgccat tttcgccagc ctgcttgcaa
     7981 taggccatac tgttgaccca cttcggaagg tcacaccaca ggaacaatcc gccttcgggc
     8041 tcctgataaa cgcactcttt catcttctca ttcaggcagg aggtcatgat tgacagtttg
     8101 tgcctgtaaa gctcacggat ggagtcaata tgtgcgtcaa aatcgtactg attcatataa
     8161 tgctcggcaa gaacctggaa gaacgagccg gtgtgaacat cgtctacctg tttgccgata
     8221 acaatcttgt caatgatcga cgcgggcgcg agagcatagc cgatacggat accggcgcaa
     8281 agaaccttcg aaaacgagcc gcagtatata acaattcctt ccgtatcaag cgatttaatc
     8341 ggaggaatat cctgaccctt gtagcgaaga tcgccatagg ggttatcctc aatgatgatg
     8401 acgccatatt tcttggccag ctcatagatt gcttttctct tctcaagcga aatcgaaatt
     8461 cccgtcgggt tctggaaggt cggaacaaca taaatcagct tgacgttttt ttcttccttc
     8521 agggattttt ccaacatggc aatgttcatg ccgtcttttt ccatttcgat gccgacaagg
     8581 ttaatgttgt aagactggaa gttgttcaaa gcgcccatat agctcggatt gtcgcagatg
     8641 accgtatcgc cctcgttaag aaaggttctg ctggaaagat caattgcctg ctgtgagcct
     8701 gatgtaatca caatttcatc aaaatctctg acacaattgc atttttccat tcttttttta
     8761 atcgtttccc ttaacggctt ataaccttcg gaaacgccgt attgaaatac ttcggcggcg
     8821 tgatcgctga taatatgttc cgaaatttta tgtatagcgt caatcggaaa tgattcggct
     8881 gaaggatttc cgtcggcaaa ctgaatgcat tttgttccct caaaatactt gacgatttcc
     8941 cggatagcgg aaggcttcat accattcgtg cggttcgaaa atacataatc catactctta
     9001 ttctccttat ttcatataat aatttaaaaa acgaaaggca gtttgtgaac ggaaatcttc
     9061 gaccgggaat caaacgaggc tcgatccaga tcaaaaggcc agccgttaat ccaggtgctg
     9121 aatatgatgc gaaagagcgg cggcccaaca aaaaataatt gtattttaca gccaaaccgg
     9181 caggaatata caatatacaa taaaatcagg taataaaaat ggcgcagtac gggtcttgac
     9241 ccatcatggc gtcatttgga tgaaacatgc ctgatcagaa tatactccgc ggattaaaac
     9301 agagccttca tttgaacagg ttgctgaatc catagtgcaa tatatcataa acatttattc
     9361 ataatgctta aatttcatta agaattcagt cgaccccaaa aatgccctga cctcttataa
     9421 atgggaggat tgtcacccaa aagcgccttt tttacgctta taacaacagt atcattatat
     9481 tacgattatg caattgtgtc aatataaaca ttcactttgt acgaatgacc tgcacaaagc
     9541 gggagtgttg tataatatat acaatccatg acttgagcgg atttcaattt caaagcacga
     9601 aaagaacgtc cgtcgtcggg tccgacggtc cgttttgcct taacaaagaa aaatgccgcc
     9661 ggcgcaaatc gccggcggca ttggcagaaa ttcaatgaag tttattcgta acgggcgcca
     9721 tgatcaaacg caccgggaac catacaggtt ttaatggcga acttggatcc ttcgaccaaa
     9781 cagtggggga tcagcgtatt ctgatcggtt ccctgctttt taccccgcac atagtgcacc
     9841 ataaaggatg ttgcaaggga atctcccgct cccatggtat cgataacctc ggcatcgacg
     9901 gccggctcaa agaagtaacg gctgccgtta aaggccatgc agccgcgagg tcccatcgtt
     9961 gcgacgacaa tcttacagcc gtagtcggaa atctttttca gtattacgcg aatttcctcc
    10021 tcggtctgat cgctgcagga taagaaagca tagtcaacat ccggagcaac ctttttaaag
    10081 taatcatccg tcgagccatg agaaaaatca aatgaaactt caacaccggc ctgccggatc
    10141 ttggacaatt cgctttccgt atagctgttg tttcccgtat ggacaaggtc aaaggtctta
    10201 atatattcga gatcaaaacg atcgagaata aagggattcg tttttcggac tccacccttg
    10261 ttgctgccga taaatacacg gttaccgttt tcaagcgtta ctttggcgca gccgttttct
    10321 cccggaatct gacggcattt gaatgtctcg atattgagtt cgcccagcgt atgaatcaca
    10381 tgctctgctt cactgtcggt tccgaaaatg cccatatatg cggatgattc cacaccattc
    10441 tgttttgcgt agacagcaaa gttgacacag ttgccaccgg gatacatcgt tttaatatgc
    10501 tcgtatttat cgacgacgtt gtcgccgaag cctaaaactc taaccattcg cagatcgctc
    10561 cttaaatttt attataaaaa tcggcgcaaa aaggttaaaa caggctgatt gccccgataa
    10621 tcaacgccga aagatctttc aggtcaccgg aatcagccgc ttcatccggg gtttgatacc
    10681 cggcaaccgg cagacaaaca gatgccgtct tgaggttaaa gccattttta accacaccgg
    10741 atatcagtga aaaaccatgg gatgcgacct ctctctgcag ctggatatgg attcgtgagg
    10801 cggcgttttc aaatgccgcc acaagatcag gatgcgtaac ggacgccgga tcccgcatat
    10861 gtaaaacagg cccgaaaccc ggatgtatac gatgatcggc cggagaagtc ccaatactta
    10921 cagcaatatc gggccgaaag tcgaaggaag cggttttggc cccccgggct tccgccacac
    10981 tgtgggaagt gaaggcaaag caaaaattgc ccggcgcttc tgcagggata cttttaatta
    11041 cgtcaatcag acagctgcat ccggctcgct ggtgcagagc cgatgacagt aatgcgccgt
    11101 tttccgaata actgcctttt aaaacagcta cgtcccctat acctgcccgc ccgccgtcag
    11161 agagccgaat ataataatcc cgcgattcgc cgccggccga ttcatgttcc ggcagcaggg
    11221 ttcccaccga accgtcctca aaaacaacct cggattcgga taagggtctc agatcgagaa
    11281 tcccttccac agagaatttc aggcggccgt tgtccggcgc gtaggtcacg atcatgcagg
    11341 gttgatccat agccgcgccg atccatactt ttttgccgag gcctttttta tgggcaatca
    11401 gattgccaag tttatcaata cagatttcgt cgacaaaagg ggaaatctcc ttttcaatga
    11461 tcgctgctac cgcctttgtg ttggccggag ggccgtaact cttacaaagg cgttttaaca
    11521 cttcgttcat gctccgatcc tcccgctttc aatctctttt agaaataaat caacaagtct
    11581 tatggtattt ctaaaatcct ccatgctggt ttcacagatg ggtgaatgaa tgtatctgca
    11641 aggaacggaa atggtgatgc atcttgcgcc tgagccggcg gtatggatgg ctcctccgtc
    11701 gtttcctccc ccggtgatcc gccttctctg gaaaggaacc ccgttttttt cagccagttt
    11761 gcaggcagcc cggcttaact ccagatcaaa atgggaggtt ctatccgcaa ttgagatagc
    11821 gggtccttca cccagtgaag tcgaatgccg ataaggttcg actacggata cgttcgagca
    11881 aacggttcct tcaagaacga tgccgatatc cggccgaacc agacccgccg ccgcaaacgc
    11941 gccgcgctcg ccaatctcct cctgcaccga aaacacaccg tagaccggca tttcataatg
    12001 gtttttaagc gtttcggcca gaacaccgca tcctgtccgg tcatccaggg ccttggactt
    12061 tatccgcccg tcgccaaagt cctcaaaccg ggttgtaaaa attgcgccgt ctccgacacg
    12121 gacatgtttt ccggcttcct ccgaagacct tgcgccgata tcaatgtagg attcacgata
    12181 actgcggccg gtccggatga ctccgtgaat acgatttttc ccaatgtaaa tcgttttgga
    12241 tgcgtcgact ttcagatcaa tccgcccaag gctttcaaag ccaagggttc cgtcacggtg
    12301 gatttcagta atcaggaatc ccacttcgtc catatgggca caaatcatga ctttcgggag
    12361 gtctgccctc ccgtttttaa ccgcgatcat gctatttaac ccatctcttc tgatttcgtc
    12421 aacataaggg ccgatctctt ttttcagaat ggcccgaacc tctccctcct gcccggtaac
    12481 accccaggcc gaagccaaat ccttcagcag caaagcagtt tcccccaatc cgctttttcc
    12541 agaatagtga gatagcccga cagcagttca gccgccgttt ccagatcgcc caagttggcc
    12601 tcttccacag aagaatagcg gtattttacc gggatggaaa tcaccgcaag tggaattccc
    12661 ccgcaggcta cctgaagcgg ccaggcctct gattcttccg gttcggccgc ggccctgagc
    12721 tgatagttga tggaagaaga ttctgcaaat ttgatcaggg cgccggtaag ttccgggtca
    12781 gaggtagggc ctgtgaaaat cgcgggcccc tcg
//
LOCUS       DATALY010000027         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_92076,
            whole genome shotgun sequence.
ACCESSION   DATALY010000027 DATALY010000000
VERSION     DATALY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92076"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1723)
                     /locus_tag="VHR42_01035"
     CDS             complement(182..1723)
                     /locus_tag="VHR42_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3025807.1"
                     /translation="MTFFELSTKMLFANFRRYRLYFLCNVFSIVLFYCMASLFTNQSF
                     MNGNVVDSSISSNIYAPSLFTGIFVALFIPYSYHAFMKNRKQEYGILMTLGMSETEVL
                     ANMLLENCVTAGLSLISGLILGTFVSFLFYFIIQQIIGLAGLRWYFNADSYKWTVILY
                     GVTMLLTLVTGILGFLKTQLTDLMKEKFRAEKKGKSLPGLFAAGAVLVIVSVVIGYGH
                     DPSNILLASMALMFAGLWLTINHAESAEQFIFKRNPDYRRRHILGISFVRQHDASRKM
                     VGILAAWLIGFSVFFAGFCAISYPSFTENAIHYSPYDLVYSRIFGKNQAADSEIKNLL
                     SQNGVSVKALKQVDYLRSGAFNLLSVSEADKNLNCNYQIPKGRFLTVFQYDLNDGYQH
                     DPVAAQTIPFHCGHEELKLQSAGSSVRILFNQNPTFADYTLILNDADYGKIASESHDF
                     WRGTMKLYSFHDWKNSEKGVNAVRNYLLEKNQGGINPSRNIIRQPPELKPTQLRNSPR
                     NFLFF"
     gene            complement(1710..>2459)
                     /locus_tag="VHR42_01040"
     CDS             complement(1710..>2459)
                     /locus_tag="VHR42_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3025808.1"
                     /translation="IKDVWKSYQLRNKEEITILKALNLQVNQGETVAIMGPSGSGKTT
                     LLNIISGVDRPDKGGVWIEGQSLADMNKAETALFRRRRLGMVFQDFNLIESLSVKENI
                     LLPMILEKKSAEEQEEQSEKILKILGIEDIQAKNITEISGGQKQRAAICRALINNPAV
                     LLADEPTGNLDSKSTREVMHHFDHINHEMKTSILMVTHDTFAASYCNRAVLLKDGEFV
                     ADIVKTGARKAFLDAITEMLALTGGEQDDIF"
BASE COUNT          651 a          602 c          502 g          704 t
ORIGIN      
        1 atcacctgcg gcatatagta acagaggttt ttatgccgta tcatttcgag catttctttt
       61 tccgttactc caatccggtt cagaccggac agcattcgct gttcttcttc cgattctgcc
      121 tttatcttga aatgaatcat aatatcggaa gcaccacaga ataaaaccac aataaaaaac
      181 attagaaaaa caaaaaattc cgcggactgt ttcgcagttg tgtaggtttc aattctggag
      241 gttgccgtat aatatttctg ctcggattga tccccccctg attcttttcg agtaaatagt
      301 tccggacggc attgacaccc ttctctgaat ttttccagtc atgaaaagag tataacttca
      361 tggttcctct ccagaaatca tggctctccg atgctatttt gccgtaatcc gcgtcgttca
      421 atatcaacgt gtaatccgca aaggtcggat tttggttaaa caggatccgg acactgcttc
      481 ctgccgactg caatttcagt tcctcatgac cgcaatgaaa aggaattgtc tgagcggcaa
      541 ccggatcatg ttggtaaccg tcattcaaat catattggaa aaccgtcaga aacctgcctt
      601 tcggaatttg ataattgcaa ttcaggtttt tatccgcctc cgataccgaa agcagattaa
      661 aagcgccgct tcttaaataa tccacctgct tcagggcttt gacggagacg ccgttttggc
      721 tcaaaagatt tttgatctcg ctgtcggcgg cctggttttt tccgaaaatc cgggaataaa
      781 ccaaatcgta tggtgaataa tggattgcat tttcggtaaa gctcggatac gatattgcgc
      841 aaaaccctgc aaaaaagacg gaaaatccaa tcagccatgc cgccaggatg ccgaccatct
      901 ttcgtgacgc atcgtgctgc cggacaaaag agataccgag aatatgccgc cgcctgtaat
      961 cggggtttct cttaaaaata aattgctccg cgctctccgc atgattgatc gtcagccata
     1021 agccggcaaa catcagggcc atgcttgcaa gcaggatatt tgaagggtcg tgaccatatc
     1081 cgataacgac cgaaacgatg accaaaaccg cccctgccgc gaatagtccc ggcagcgatt
     1141 ttcccttctt ctccgccctg aatttttcct tcattaaatc ggtaagctgc gttttcagga
     1201 atcctaaaat accggtaacc agcgtaagga gcattgtgac gccataaaga ataaccgtcc
     1261 atttatagga atccgcgtta aaataccatc gcagaccggc taaaccgatg atctgttgga
     1321 tgatgaaata gaacaggaag gatacgaacg ttcccaatat cagcccgctg atcagggata
     1381 aacctgccgt tacgcagttt tcgagcagca tattggccag tacttccgtc tcgctcatgc
     1441 ccaatgtcat taaaataccg tattcctgtt ttctgttctt catgaaagca tggtatgagt
     1501 aggggataaa cagagcaaca aaaatcccgg tgaataaact cggcgcgtaa atgttgctgg
     1561 aaatgctgct gtcaacgacg ttcccattca taaacgattg attggtaaac agggaagcca
     1621 tgcagtaaaa cagcacaatc gaaaacacgt tgcataaaaa atacagccgg tatcgccgga
     1681 agttcgcaaa taacattttc gttgaaagtt caaaaaatgt catcctgttc acctccggtc
     1741 agcgcaagca tttccgttat ggcgtcaaga aatgcctttc tggcgcccgt tttcacgatg
     1801 tccgcgacga attctccgtc tttcagcaaa accgcccggt tacaatagct tgccgcaaat
     1861 gtatcgtgcg tgaccattag aatacttgtt ttcatttcat gattgatgtg gtcaaaatga
     1921 tgcatcactt ctcttgtgga ttttgaatcc aggtttccgg taggttcatc cgccaacagc
     1981 accgcgggat tattgatgag tgctctgcag attgccgcgc gctgtttctg tccgccggat
     2041 atttccgtta tgtttttagc ctgtatgtct tcaataccaa gaattttcag aattttttcc
     2101 gactgctctt cctgttcctc cgcggacttt ttctctaaaa tcataggcag caggatattt
     2161 tccttcacgg aaaggctttc gatcagatta aaatcctgaa agaccattcc taatcttctt
     2221 ctgcggaata atgccgtttc cgctttattc atgtcggcta aagactggcc ttcaatccat
     2281 acgccgcctt tatccggccg gtcgactccg cttatgatgt taagcagtgt cgttttcccg
     2341 cttcccgacg gccccatgat tgcaaccgtt tctccctggt ttacctgcaa attaagagct
     2401 tttaatatag tgatttcttc cttgttcctt aattgataac ttttccatac gtctttaat
//
LOCUS       DATALY010000028         6895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_610488,
            whole genome shotgun sequence.
ACCESSION   DATALY010000028 DATALY010000000
VERSION     DATALY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610488"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VHR42_01045"
     CDS             complement(<1..731)
                     /locus_tag="VHR42_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025809.1"
                     /translation="MNVTIPINIFQIMASNNANRFIYYFKRIPLIGRLMPDRVYQKVG
                     LKRNVALAALMIGFIKNFLGKGIYLGLMIVLPLALMQKGLSQPTRFQTFTQMLAFLSF
                     AVGSFLSPVLFKTDREKFICIRLMRMNPKSYLVSSALLRAISDAVYFLPALMICSALL
                     GATALHGILLTVMLGASRLVGEAFHLAFYLVFRVVLCQKTAVVSAFAIAGILAAYLPV
                     GLHAPAVLGSALLFIPVAIFVLAAGV"
     gene            complement(728..1435)
                     /locus_tag="VHR42_01050"
     CDS             complement(728..1435)
                     /locus_tag="VHR42_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3025810.1"
                     /translation="MELTIQNLSKSYGDKQVLKGVKFTFEQGKIYGLLGRNGAGKTTL
                     FNCLSGEDKPDAGEMILKTQDREGTLKPENIGYVFATPILPEFLTGYEFLKFYLDINS
                     GRIPERKPIEYYFEMVQIAPEDRHRLIKGYSHGMKNKMQMLCFLISKPPVILLDEPLT
                     SLDVVVALEIKKLLRSMRKDHIILFSTHILQLATDLCDEIVVLNEGKLEQIDHRMIGS
                     SHFEDSIINLLKDENDS"
     gene            1786..2988
                     /locus_tag="VHR42_01055"
     CDS             1786..2988
                     /locus_tag="VHR42_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX3025811.1"
                     /translation="MDIYVARQPIFDRRLNLFGYELLYRRSMNNFYEGTDDASSTAAL
                     IDNTFLVIGLDELTGGARAFINFSEEFLKKEIPLLLPKENVVVEILETVAVSGIVVDA
                     CKKLKSLGYTLALDDFAFQEEYDPLIDLADIIKVEFPGTNMDDQLHLLQKYKKKKIFL
                     AEKVETRSEYQTALQMGYDLFQGYFFSRPSILNSREIPSLSMNLIQILNELNEPEPDY
                     LKISEIIEKDLGLSYKLLKMANSFYFQSQRKITSIHQALVRLGTQEMIQWTHLMSLKS
                     VQTTENAELVKFSSIRGKFMSLLSAELKLASAGSEFFLTGILSSIDAILNRNMQEIID
                     GLPLTGRVKEALTGTDNVLRRLLDVTLDYEAAKWDQLTASQTVKQVGLDKFISLYLEA
                     VNWQREFA"
     gene            3225..3566
                     /locus_tag="VHR42_01060"
     CDS             3225..3566
                     /locus_tag="VHR42_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3025812.1"
                     /translation="MFSELFGKKNYRNLSQTEAKKKLDLGEPVVLLDVRSPEEYAQVH
                     IPGSVSLPLDRLADGISKVASKDSELLIYCQSGMRAATASRLLSSLGYTNINNIGGIQ
                     TWKYETEKGRR"
     gene            complement(3690..4367)
                     /locus_tag="VHR42_01065"
     CDS             complement(3690..4367)
                     /locus_tag="VHR42_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3025813.1"
                     /translation="MTKEQIRIFLNGQLPFFKKLPQPEAEAVLSSASGAFYRRGSMLL
                     SREKECNGLVLVKSGQLRAYFESEDGKEITLYRLLSGDICILSASCVLRNITFEVTLE
                     AEKDSEVVLIPASVWGKLSEQNPEAGLFTQALINERFSEVMWVMEQIVSKNMGQRLAA
                     FLLEQSALDDTRILSMTHETIAKNLGTAREVISRMLKYFENDRIVSLSRGKIQLEDLN
                     RLKEMSL"
     gene            4577..5917
                     /locus_tag="VHR42_01070"
     CDS             4577..5917
                     /locus_tag="VHR42_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3025814.1"
                     /translation="MKKGFNRNHLTVMALGNIIGSGVFLGSSTVISIAGPAAVLSYLL
                     GGVIMAMEVSFITEMSVVNPVPGAFRVHATEIFGPWIGFVNGWVFWFCGILGMASEVA
                     AAAIFTRFWFPGIPMWMFCIVYSALMAAINLNDPRGLSRMEGILASVKIAALIFFILF
                     GLFLTLRIIHVGSLRISNPLTSMHAFAPNGIRGILSSMILVMFSYTGTGIIGLSIADA
                     ENPEKDAPSAIRIIITSVLALFVLSALFTVLLTPWNRISSSASPFVIILQHLKIPYSD
                     SILNFIVLSAALSGLNSSMYSSSRMLNSLSRDGQAPRIFLKTNHNGVPIYALGTGSAA
                     LFLTAVLSYLIPSTVFVILATASGFLAMFNWLTISVTYYFYRKKTLKERPWKLRYKAP
                     GYPYAPWIEMALIVAILITAPLYPGQLTGLAGGILLFLGLMAIYRILKKRGFLK"
     gene            complement(6229..>6895)
                     /locus_tag="VHR42_01075"
     CDS             complement(6229..>6895)
                     /locus_tag="VHR42_01075"
                     /inference="COORDINATES: protein motif:HMM:NF025756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YheC/YheD family protein"
                     /protein_id="HEX3025815.1"
                     /translation="KDVYIKPVAGLQGRGIIRIISLDQGGFVFRYRDGEKNFEKKFRN
                     QRSAMKFLKKRTSSGNYLVQQSVKLLQYRGGPVDFRCVMQKNQTGMWKCRTIIGRCGR
                     PGSVVSNISSGGSALRGEEAIRRIRPEWDSGAVISRMEKLALSVCNALDGLGLNFGTL
                     GLDIGVDDEGIFWLIEINNRDPDTTIALNIKDKKLFCRLKEGPLFYAKALAGFSSTDE
                     EES"
BASE COUNT         1816 a         1761 c         1554 g         1764 t
ORIGIN      
        1 acgccggcgg ccaaaacaaa aatggcaacg ggaatgaaaa gcagcgcgct gcccaaaacg
       61 gcaggcgcgt gaagcccaac gggaagataa gccgccagaa ttccagctat ggcaaaagca
      121 gaaacaacgg ctgttttttg acacagcacg actcggaata caagataaaa cgcaagatga
      181 aacgcttccc cgaccagacg ggaagcgccg agcatgactg tgagcagaat gccgtgcaga
      241 gccgttgcgc ccaaaagcgc cgaacaaatc ataagggccg gaagaaaata gacggcgtcg
      301 cttatcgcgc gcagaagagc cgaagacaca agataacttt tgggattcat tcgcatgaga
      361 cggatgcaga taaatttttc cctgtctgtc ttaaacaaaa ccggcgacag aaaagatcct
      421 accgcaaagc tgaggaaagc aagcatctga gtaaacgttt gaaaccttgt cggctgtgac
      481 aggcccttct gcatcagcgc aaggggaaga acgatcataa gaccgagata gatgcccttg
      541 cccaaaaagt tcttaataaa accaatcatc agcgcggcca acgcgacgtt ccgcttgagc
      601 ccaacctttt gatagacacg atccggcatg agcctgccga tcagtggaat ccgtttaaaa
      661 taataaataa aacggttcgc attattggac gccataattt gaaagatatt aatcggtatc
      721 gtaacattca tgaatcattc tcatctttca gaagattgat aatgctgtct tcaaaatggc
      781 tgcttccgat catacggtga tcaatttgtt caagttttcc ctcattcagc acaacgatct
      841 catcgcaaag atcggtagcc agctgaagaa tgtgtgtgga aaaaaggata atgtgatctt
      901 ttctcatgga acgaagcagt tttttaattt ccagtgcgac caccacatcc agagaggtca
      961 gcggttcgtc gagcaaaatc accggcggct tcgaaataag gaagcaaagc atctgcattt
     1021 tattcttcat tccatgggaa tagcccttaa tcagacggtg ccggtcttcc ggcgcaatct
     1081 gcaccatttc aaaatagtac tcaatcggct ttctttccgg gattctgccg ctgttgatgt
     1141 ctagataaaa ttttaaaaac tcatagccgg tcaggaactc cggaaggatc ggcgttgcaa
     1201 aaacataccc gatattctcg ggcttcagcg tgccttcccg atcctgagtc ttcagaatca
     1261 tttcacccgc gtcgggcttg tcttcgccgc tcaggcagtt aaacagcgta gtttttcctg
     1321 cgccgtttct tccgagaagc ccgtaaattt tgccctgctc aaaggtaaat ttcactccct
     1381 tgagcacctg tttgtcacca taggacttcg ataaattttg gatggtcaac tccattttct
     1441 tcttccctcc aaggccatat ttaaaagagg ctgtcattgt cccgattgat tttttgcttt
     1501 tgtttttaga atcccgtttc tttcattata acaatttttg gatcaaaatc aaaatttgga
     1561 tattgatggt tgcttcccca ggaacaatca ggtataataa gtatatcgac attattcgtc
     1621 aaaaaagccg aaaatattaa acgttaagtc gtaaatttat gaaaataaat tcaaatctat
     1681 gaggccgtca caaattgatc ctaaaagttt ctctttttat tttgttatga aactccgaaa
     1741 cataccacgg atttctgtct tgctattatg taaggagtga tatacatgga tatttatgtc
     1801 gcccgtcagc cgattttcga tcggcgcctg aatcttttcg gttatgaatt attataccgg
     1861 cgcagcatga ataattttta tgagggaacg gatgacgcca gctctaccgc ggcattgatc
     1921 gacaacacat tccttgtcat tggactggac gaactcaccg ggggggcaag ggcttttatt
     1981 aatttttcgg aagaattttt aaaaaaggaa atccctttgc tgctgccaaa agaaaacgta
     2041 gtcgtcgaaa ttctggaaac cgtcgcggta agcggcattg tcgttgatgc ctgcaaaaag
     2101 ctgaaatctt tagggtatac ccttgctctt gatgattttg cctttcagga ggagtatgat
     2161 cccctgattg atctcgcgga catcatcaaa gtagaattcc cgggtactaa catggacgat
     2221 cagctgcatc tgctgcaaaa atacaaaaag aagaaaatat ttctcgcgga aaaggtcgaa
     2281 accagaagcg aatatcaaac cgcgcttcaa atgggctatg acctctttca gggttacttt
     2341 ttcagcagac catccattct caactccagg gaaattccat cattaagtat gaatctgatt
     2401 cagattctca acgagctgaa tgaaccggag ccggattatc tgaaaatttc ggaaatcatt
     2461 gaaaaagatt tgggcctttc ctataaactc cttaaaatgg ccaactcgtt ttatttccaa
     2521 tctcagcgaa aaatcacctc catccatcag gcgctcgtcc ggctcggtac acaggaaatg
     2581 attcagtgga ctcacctgat gtctctcaaa agtgttcaga ctaccgaaaa tgcggagctt
     2641 gtcaaattct cgtctatccg ggggaaattt atgtctctcc tgtcggccga gctcaaactc
     2701 gcaagcgctg gttcggagtt cttcctcacg gggattcttt cgtcgataga cgccatcctg
     2761 aatcggaata tgcaggaaat catcgacggc cttcccctca ccggaagagt caaagaagcc
     2821 ctgacgggca cggataacgt attaagaaga ctactggatg tcactctcga ttacgaggcc
     2881 gcaaagtggg atcagctaac tgcctctcaa accgtgaaac aggtcggatt ggataaattt
     2941 atcagccttt acctcgaggc ggtcaactgg cagcgggaat tcgcataagc atacaggccg
     3001 gccacaaaca ggtggccggc ctctctttat ccttccggac cggcagagat gtcctgatag
     3061 aatccgtgtg acgttgtcac tgttaacaga cgacaggcag gatatactgt tcatgaaaga
     3121 agttcaagcg gcatacggaa tccatttttt atatttccgc gttttcagcc gacaatttcg
     3181 ccgctcggat ggattgaaaa tcaacgaccg gaaaggtgaa aacaatgttt tcagaattat
     3241 ttgggaaaaa gaactaccgg aatctgtccc agacagaggc caaaaagaaa ctcgatttgg
     3301 gagaaccagt tgtccttctc gacgtcagaa gcccggaaga atacgcacag gttcatattc
     3361 ccggcagcgt ttcgctccct cttgaccggc tggcagacgg catttccaaa gtcgcatcaa
     3421 aagacagcga gctccttatt tactgccaga gcggcatgcg tgcagcaacc gcaagccggc
     3481 tgctttcctc tctcggctac acaaacataa acaatatcgg gggaattcaa acctggaaat
     3541 atgaaaccga aaagggacgc cgttaatcgg gctgagaact cctgccgttt caacgcctga
     3601 cctcctgtaa aacaaaaagc gggaagcccc atgaaggctt cccacccaag acgctgttcc
     3661 ttgaaagcgg cattccgccg ttcaaccggt cagaggctca tttccttaag cctgttcaaa
     3721 tcctcaagct gaatttttcc ccgggacaga gaaacgattc ggtcattttc aaaatatttt
     3781 aacatacggc tgatgacctc ccgcgcggtt cccagattct ttgcaattgt ttcatgcgtc
     3841 atgctcaata tccgggtatc atccagcgcc gactgctcca ataaaaaagc cgccagccgc
     3901 tgccccatgt ttttggaaac gatctgttcc atcacccaca tgacttcaga aaacctctcg
     3961 ttgatcagtg cttgtgtaaa aagcccggct tccggattct gctcggaaag ttttccccag
     4021 accgacgccg gaatcagtac aacctcgctg tctttttccg cctcaagagt cacttcaaac
     4081 gtgatattcc gcagcacgca ggatgcggaa agaatacaga tatcacccga aagcagtcgg
     4141 taaagcgtaa tctccttacc atcctccgac tcaaaatacg ctctcagctg cccgcttttc
     4201 acaaggacca ggccattaca ctccttttcc ctgctcagga gcatactgcc ccgccggtaa
     4261 aaggccccgg atgccgaaga caaaacggct tccgcctccg gctgcggcag ttttttaaaa
     4321 aacggcagct gcccgtttaa aaatattctg atctgctctt ttgtcataag gacacctccg
     4381 aactttcctg tctgaaaagg ttctgacgga tgctcatttt tagtatagca tgcgccgggc
     4441 ccaatgaaaa gcaacaaaaa taaaacatag ccccaaacgg ttgggcagtc ttaaaaaagg
     4501 ctcggctggg catgataaaa aaacggagaa aattcattca gaacggtcag gcattaaaag
     4561 caacggagtg ttttatttga aaaaggggtt taacagaaac cacctgacag tgatggccct
     4621 gggcaacatc attgggtcag gggtattcct cggcagctcc acggtcatat cgatcgccgg
     4681 gcccgccgca gtattgtcct atctgctcgg cggtgtgatc atggcaatgg aagtgtcctt
     4741 tattactgag atgtcggttg tcaatccggt gccgggtgcg ttccgcgttc acgcaaccga
     4801 aattttcggg ccctggatcg gttttgtcaa cggctgggta ttctggtttt gcggcattct
     4861 tggaatggca agtgaagtgg ccgccgccgc aatattcaca aggttctggt ttcccggcat
     4921 ccccatgtgg atgttctgca tcgtttattc cgcgttaatg gccgcaatca atctcaacga
     4981 cccgagaggg ctgagcagaa tggaaggaat tctcgcgtct gtcaagatag ccgcgctgat
     5041 tttctttatc ctgttcggtc tttttcttac gcttcgaatc atccatgtcg gctccctccg
     5101 gatcagcaat ccgttaacat caatgcatgc ctttgcgcca aacggcataa ggggaattct
     5161 gtcttcgatg attcttgtga tgttctccta taccggcacc ggaatcatcg gcctttcgat
     5221 cgcggacgca gaaaatcctg aaaaggacgc tccctccgcc atccgtatca ttatcacctc
     5281 cgttttggcg ttgtttgttc tttccgcact gttcaccgtt ctgctgacgc cctggaaccg
     5341 tatttcctcc tccgcaagcc cgtttgtaat aattcttcag catcttaaga ttccctattc
     5401 cgacagcata ctgaatttta tcgtgctcag cgcggctctc tccgggctga actcctccat
     5461 gtacagctcc tcccgcatgc tcaactcact gagccgtgac ggacaggctc cccggatatt
     5521 tttaaagaca aatcataacg gtgttccgat ctacgctctg ggaacgggaa gcgcggcgct
     5581 gtttctgacg gccgtgcttt cctatctgat tccgtcgacc gtctttgtaa ttttggctac
     5641 ggcaagcgga tttcttgcaa tgttcaattg gctgaccata tcggtgactt attactttta
     5701 tcgaaaaaag acgttaaaag agcggccctg gaaactgcgt tacaaggctc cgggctaccc
     5761 ttacgccccc tggatcgaaa tggcgctgat tgttgcaatc ctgattaccg ccccccttta
     5821 cccgggacag ttaaccggtc ttgccggagg catactgtta tttctggggc tgatggcgat
     5881 ttatcggatt ctcaaaaaaa gaggctttct gaaataacct cgttcaaaca aatggattgc
     5941 gccgtccgct gtggcagacg gcgcattttt tcttccgttg ttttcgcatc agtcggaaat
     6001 tatgatttga attccaggct catgcggtga caacggaaga aagatgatcc gggtccttag
     6061 aaagaaacat cccatatctc agggtatgac gcatgtctgt gaaaagtgcg tcatacaggc
     6121 tcaatgaaag cggagccgca aaggggaaaa acttcccgca gctcctaaga agggcggcca
     6181 cagcggtatc gtttccggct gcccacacgc tccgcggcct gtttcctttt aggattcctc
     6241 ttcatctgtc gaggaaaaac cggcaagagc cttggcataa aacagcggac cttccttgag
     6301 ccggcagaaa agttttttgt ccttgatatt cagagcgatc gtggtgtcgg gatcacggtt
     6361 attgatttca atcagccaga aaatcccttc atcgtccaca ccgatatcca ggccgagcgt
     6421 gccgaaatta aggccgagac catccagcgc attacatacg gacagcgcca gtttttccat
     6481 acggctgatg accgctccgg agtcccattc gggccggatt cgccggatcg cctcttcgcc
     6541 ccggagcgcg cttcccccgc ttgagatatt gctcacaaca ctgccggggc gtccgcatcg
     6601 gccgatgatc gtccggcatt tccacatgcc ggtctgattc ttttgcatca cgcaccgaaa
     6661 atccaccggg ccgccgcggt attgcagcag tttaaccgat tgttgaacca gatagttccc
     6721 ggatgaggtt cgctttttca gaaatttcat tgcggaccgc tgatttctga attttttctc
     6781 aaaatttttt tctccatccc gataacgaaa aacaaagcca ccttgatcca gactgattat
     6841 tctgataata ccccgaccct gaagccccgc aaccggttta atatagacat cctta
//
LOCUS       DATALY010000029         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_696938,
            whole genome shotgun sequence.
ACCESSION   DATALY010000029 DATALY010000000
VERSION     DATALY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696938"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VHR42_01080"
     CDS             complement(<1..981)
                     /locus_tag="VHR42_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX3025816.1"
                     /translation="MNSEIKAAVEKFEKLLESQLLRIEQMKQQKEFINYDKLDRIIIG
                     ECGGDGIGPVITNEASRVLKHLLAKEIESGRVVFKQIDGLTIENRAKVNKAIPDDVLE
                     ELKQCHVILKGPTTTPRAGDPWPNIESANVAMRKELDLFANVRPVKVPDKGIDWTFFR
                     ENTEGSYAVGSSGVHVNPDLAVDFTITTAEGTDRIARLAFDYAKRNGKNHVSVVTKAN
                     VIKTTDGKFLNICQNIAKEYPSITTDDWYIDIMTAKLIDEKRRKDFSVFILPNLYGDI
                     ITDEAAEFQGGVGTAGSANIGKRYAMFEAIHGSAAFSDDDPVEFIIIDEFF"
     gene            complement(1019..>2104)
                     /locus_tag="VHR42_01085"
     CDS             complement(1019..>2104)
                     /locus_tag="VHR42_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3025817.1"
                     /translation="AIKVDQVCIGSCTNSSLQDMMKVATILRGSKVHPNVSLSISPGS
                     KQVYTILAQNGALADLISAGARILECACGPCIGMGQSPNSGGVSLRTFNRNFEGRSGT
                     ADAQVYLVSPETAAASAISGVLTDPRSFGSEPEIRQPETFYINDNMIEAPASPEKAEK
                     VEILRGPNIKPFPVNTELSENIEAGCLLKVGDNITTDHIMPAGAKILPYRSNIPKMSE
                     FCFAVCDKTFPQRAKAAGKGMIVGGANYGQGSSREHAALVPMYLGIKAVIAKSFARIH
                     FANLINAGILPLTFKDADDYDKISQGDTLALTGIRNAVENGGPIILENKTSDTKTELS
                     CELSKRQREMLLAGGLLNFTREQARNA"
BASE COUNT          424 a          521 c          591 g          568 t
ORIGIN      
        1 aaagaattca tcaattatga taaactcgac cggatcatca tcggagaatg cggcggagcc
       61 gtgaatcgct tcaaacatgg catagcgctt gccgatattt gcgctgcccg ccgtgccgac
      121 gccgccctga aattccgcgg cctcgtcggt gatgatgtcg ccatagaggt tggggaggat
      181 aaagacgctg aagtcttttc tccttttctc gtcaatcaat ttggcggtca tgatgtcaat
      241 gtaccagtcg tcggttgtaa tcgagggata ttccttcgcg atattctggc agatattgag
      301 gaattttccg tccgttgtct tgatgacgtt ggccttggtg acaaccgata cgtgattttt
      361 accgtttctt tttgcgtagt caaaggcaag gcgggcaatc cggtccgtac cttccgccgt
      421 tgtgatggta aaatcgacgg caaggtcggg gttcacatga acgccgctgc tgccgaccgc
      481 ataagagcct tcggtgtttt cgcggaagaa agtccagtca atccctttgt cgggaacctt
      541 gacggggcgg acgtttgcaa aaagatcaag ttccttgcgc atcgcgacgt tggcgctctc
      601 aatattcggc cagggatctc ccgcccgggg agtagtggtc ggccctttga ggatgacatg
      661 gcactgtttg agttcctcaa gaacgtcgtc gggaatcgct ttgttgactt tggctctgtt
      721 ttcaatcgtc aggccgtcaa tctgtttgaa taccacccgg ccgctttcaa tttctttggc
      781 aagcaggtgc ttgagcaccc gtgacgcttc gttggtaata acggggccga ttccgtcgcc
      841 gccgcattct ccgatgatga tccggtcgag tttatcataa ttgatgaatt ctttttgctg
      901 tttcatttgt tcaattctga gaagttgcga ttctagaagt ttttcaaatt tttccacagc
      961 agctttgatt tcggaattca tggttccaac atcctttcga ttttcggttt gccgtttatt
     1021 aagcgttgcg ggcctgctcc cgcgtaaagt tgagaagacc gccggcaagc agcatttccc
     1081 gctgccgttt cgacagctcg cagctaagct ctgtttttgt gtcagacgtt ttattttcaa
     1141 gtatgatcgg cccgccgttt tcaacggcgt ttcgaattcc ggtcagggca agcgtgtcgc
     1201 cctggctgat tttatcataa tcatccgcgt ctttaaaagt taacggcaga atgccggcgt
     1261 taatcaggtt tgcaaagtgg atgcgggcaa acgattttgc aatgaccgct ttaattccca
     1321 gatacatagg tacaagggcg gcgtgctccc tggaagatcc ctgcccgtaa ttggcgccgc
     1381 cgacaatcat ccccttgcct gccgcttttg cgcgctgcgg gaaggttttg tcacagactg
     1441 caaagcagaa ttccgacatt ttggggatgt tggagcggta gggaagaatt ttggcaccgg
     1501 cgggcatgat gtgatcggta gttatgttgt cgccgacttt cagtaagcag ccggcttcga
     1561 tattctcaga aagctccgtg ttgaccggga acggcttgat attcggcccg cggaggattt
     1621 ccaccttctc cgctttttca ggggaagcgg gcgcttcaat catgttgtcg ttgatataaa
     1681 aggtttcggg ctggcggatt tccggttcgc tgccaaagct tctcgggtcg gtcagaacgc
     1741 cggatatcgc cgaggccgcc gcggtttcag ggctgacaag atagacctgc gcgtcggctg
     1801 ttccgcttct gccctcgaaa ttacggttaa aggttctcag ggagacgcct cctgagttgg
     1861 gggactggcc catgccgatg caggggccgc aggcgcattc caatatacgg gcgccggcgg
     1921 aaatcaggtc ggccagagcc ccgttctggg caagaatagt atagacctgt ttggagccgg
     1981 gcgaaatgct caggctgacg ttggggtgaa ccttgctgcc gcgtaaaatg gtggcgacct
     2041 tcatcatatc ctgaagcgaa gagttggtgc aggagccgat gcagacctgg tcgaccttga
     2101 tcgc
//
LOCUS       DATALY010000030         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_179410,
            whole genome shotgun sequence.
ACCESSION   DATALY010000030 DATALY010000000
VERSION     DATALY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179410"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VHR42_01090"
     CDS             complement(<1..328)
                     /locus_tag="VHR42_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX3025818.1"
                     /translation="MGSDGSIPLQLILLFILILINGFFAASEIAIITINDKKLKKLAE
                     DGNDNAILLSQLLTKPSSFLAAIQVGITLASLLSSAVASESFADRITAYFAVFRFPVH
                     RRPSSTG"
     gene            970..>2282
                     /locus_tag="VHR42_01095"
     CDS             970..>2282
                     /locus_tag="VHR42_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX3025819.1"
                     /translation="MARKMKTMDGNTAAAHVAYAFTDVAGIYPITPSSVMAELADKWA
                     ADDRNNIFGQKVKVVEMQSEAGAAGTVHGSLNGGALTTTFTASQGLLLMIPNMYKIAG
                     ELLPGVIHCSARALASHALSIFGDHSDVYACRQTGFAMLCSSNVQEVMDLAAVAHLSA
                     IKGRVPFLHFFDGFRTSHEVQKIEIWDYEDLKSMADMDAINAFRKRALNPEHPVLRGT
                     AQNPDIFFQAREACNTYYNAVPAIVEEYMGKVNKLTGKDYKLFNYYGANDAENVIVAM
                     GSACDAIDETIDYLNAKGEKTGLIKVRLYRPFSAEHLLAAMPKTVKKITVLDRTKEPG
                     SLGEPLFLDIVAALKNTEFDAVPIYSGRYGLGSKDTTPGQIVSVYRNAVSATPKKRFT
                     IGIEDDVTNLSLAVTENPDTTPAGTHSCKFWGLGSDGTVGANKNS"
BASE COUNT          596 a          557 c          549 g          580 t
ORIGIN      
        1 ggccagttga agaaggccgc cgatgaacgg gaaagcggaa aaccgcaaag taagcggtaa
       61 tacgatcggc gaaactttcg gatgctacgg cgctcgacag aaggctggcc agcgtgatgc
      121 ctacctgaat ggcggcgaga aaactgctgg gcttggtcag caactgagaa aggaggatgg
      181 cgttatcgtt gccgtcttcc gccaatttct taagcttttt atcatttatg gtgatgattg
      241 cgatttcgga agcggcaaaa aagccattga ttaatattaa aatgaacagc agaatcagtt
      301 gtagggggat actgccatcg gaacccattt acaaactcct ctcaaaaagc gaaaaatacc
      361 gaaaaaagca gccgctgcga tgatcctcca gcagttgcgt atctttttct aacattatat
      421 taatggtttt ccactgtaat gtcaaatacc ggaatacgct tattttcaag cttttatggc
      481 cgacgtgggt tgctgcgccg cgtcaccgat ttcaatccca gtcgcaagct gttttacgcg
      541 tttccaaagc ctctggggcg ttcgcagttt aatggctggc aataaaaagt tgtagcaaca
      601 atgaaaagtt ataaattgtt gatttgatgt ttacagaaat aaagtaaagt gctaatatat
      661 tctttgaata attaaatcaa ctttgttaaa aaataaacta tctaatagta agaagagagc
      721 atttccgccc agtttatgat tatgcatttc ggctgggatt tatgcttgtc aaagaggaaa
      781 tgattattcg aacgccggcc tttccagggc ggctgaccat cggcttatgc agctctgcgg
      841 ctggttattc gaagcgagct gttttgagca tgcgcattcc gttttccggg ttgatgcgta
      901 tcgataaagg tagctgctat aaattctttt tgtagtagta ttattaaatt aaagggagga
      961 acttattcca tggcaagaaa aatgaaaacc atggacggga acaccgctgc tgctcatgta
     1021 gcatatgcgt ttaccgacgt ggcgggtata tacccgatca cgccctcgtc cgtcatggct
     1081 gaactggcag acaaatgggc ggctgatgac agaaacaaca ttttcggcca aaaggttaaa
     1141 gttgttgaaa tgcaatcaga agccggtgct gccggaaccg ttcacggctc actcaacggc
     1201 ggtgcgctca ccacgacatt cacggcttca cagggtctgc ttcttatgat ccccaatatg
     1261 tataagattg ccggcgagtt gctccccggt gtcattcact gttctgcccg tgcgcttgct
     1321 tcccatgcgc tctccatttt cggagaccat tccgatgttt atgcctgccg ccagactggt
     1381 ttcgccatgc tctgctccag caacgttcag gaagttatgg atcttgccgc cgttgcgcat
     1441 ctttcggcga ttaaaggcag agtaccgttc ctgcattttt ttgatggttt ccgtacctcc
     1501 catgaagtcc agaaaattga aatctgggat tatgaagatc tgaagagcat ggccgatatg
     1561 gatgcgatca acgcattccg caaacgtgct ctgaaccctg agcatcctgt gcttcgcggc
     1621 acagcgcaga acccggatat tttctttcag gcaagagaag cctgcaacac ctactacaat
     1681 gctgttccgg ctattgtcga agaatatatg ggcaaggtca acaagctgac cggcaaggat
     1741 tacaagctgt tcaattacta tggcgcgaac gacgccgaaa atgttatcgt tgcgatgggc
     1801 tcggcctgcg acgccatcga cgaaacgata gattacctca acgccaaagg cgaaaaaacc
     1861 ggcctgatca aagtccgtct ttacagacct ttctcggctg agcaccttct tgccgcaatg
     1921 cccaaaacag tcaagaagat taccgtcctc gaccgtacca aggagcccgg cagcctcggc
     1981 gagccgcttt tccttgatat cgttgccgct ctcaaaaaca ccgagtttga tgcggttccg
     2041 atttattccg gacgctatgg cctcggctcc aaagacacca caccgggcca gattgtttcc
     2101 gtctatcgca acgctgtttc ggcaaccccg aaaaagcgtt tcaccatcgg tatcgaagac
     2161 gatgttacca atctgtcgct tgcggttacg gaaaatccgg ataccacgcc tgcaggcacc
     2221 cattcctgca aattctgggg tcttggctcg gacggaaccg tcggcgccaa caagaactcc
     2281 at
//
LOCUS       DATALY010000031         5511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_611087,
            whole genome shotgun sequence.
ACCESSION   DATALY010000031 DATALY010000000
VERSION     DATALY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611087"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="pflA"
                     /locus_tag="VHR42_01100"
     CDS             complement(<1..660)
                     /gene="pflA"
                     /locus_tag="VHR42_01100"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HEX3025820.1"
                     /translation="MKGRIHSFQSFGAVDRPGLRCVVFLQGCTLRCAYCHNPDTWDPA
                     DGTECSVDEAAEKILRYRPYFAANGGVTVSGGEPLLQWEFVAELFRRLRREGVHTALD
                     TAGIGDPEGTRAVLLNTDLVLCDLKFSCAEDYQQYCGGTRDVVLRFLKQTEELRVPLW
                     IRHVVVPGLADGEECILKIAEEALRFTNLQKLELLPFRKLCLPKYRAMDLPFPLESND
                     EC"
     gene            complement(660..2891)
                     /gene="pflB"
                     /locus_tag="VHR42_01105"
     CDS             complement(660..2891)
                     /gene="pflB"
                     /locus_tag="VHR42_01105"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEX3025821.1"
                     /translation="MSNQAWKNFKSGTWETEINVRNFIQTNYTPYEGDGEFLTPATER
                     TKALMKKLNRLLELEQEFGGVLDVDTETVSSLTSYRPGYLDKEHEIIVGLQTDRPLKR
                     GVNPFGGLNMTRKACEAYGYRLSDQVEKEFQYRTTHNEGVFRAYTDEIRAARHCGIIT
                     GLPDAYGRGRIIGDYRRVALYGIDRLIEEKKKDKAEVGAQNMDADHVRQLEELFQQIT
                     FLGYLKDMAALYGFDISRPAENAKEAVQWLYFGYLGAIKEQNGAAMSLGRVSTFLDIY
                     FERDLESGILTEQEAQEIFDDFVMKLRMARHLRTPEYNELFAGDPMWITESVGGMGED
                     GRTLVTKSSFRMLHTLYNLGPAAEPNLTVLWSGRLPEPFKSFTAKVSCDTDAIQYEND
                     DIMRPKFGDDYGIACCVSAMRLGKDMQFFGARANLAKLLLMSLNGGRDEKSGQQVAPA
                     RAPYEGDILDYGKVVSGMAFYRDWLAKTYVSAMNIIHYMHDKYAYEKTQMALHDTDVH
                     RFMAFGIAGLSVLTDSLSAIRYAKVKCIRDETGLIRDFETIGEYPAYGNDDDRVDSIA
                     QEQVKLFYESLKKNPTYRSAEHTLSILTITSNVVYGKKTGATPDGRKAGEPFAPGANP
                     MHNREKNGALASLNSVAKLSYDICKDGISNTFSIIPQALGHREEEQHENLVSLLNGYF
                     GQNAHHLNVNVLNRETLLEAYEHPDRYPNLTIRVSGYAVNFHKLSKEQQREVISRTFH
                     EAM"
     regulatory      complement(2977..3065)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(3163..4083)
                     /locus_tag="VHR42_01110"
     CDS             complement(3163..4083)
                     /locus_tag="VHR42_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3025822.1"
                     /translation="MKKSIVCILAAAFLFGTMEVSLKLAGASFDPIQMTFLRFLIGGL
                     CLFPFALYDLKKRQCHLTKSDWLYLFLLGFTCICVSMLLFQIGVLRTNANLAAVVIST
                     SPVFTMIFAQFFANEKFTLVKFFVLLLNVMGLIIVANPIKLMKGHTAVDGILITLIAA
                     ASFGLYTAMGKKRIAKIGGLTQSSFSFIMGSLVLLVIQLAKKGPVMKGIQPNTLPLLL
                     YLGVLVTGFGYFCYIRAIEVSGPSTASITFFIKPVFAPIIAFLILKEPITLNIILGVV
                     FVLSGSLLNLFGEKIRASFSSVASSKEEAA"
     gene            complement(4306..5331)
                     /locus_tag="VHR42_01115"
     CDS             complement(4306..5331)
                     /locus_tag="VHR42_01115"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX3025823.1"
                     /translation="MEKKDLDWANLDFSYRKTDKRFVANYREGAWDEGQLTDDDKVVI
                     SECAGVLQYSQSCFEGLKAYTTEDGRIVAFRPDMNAERMENSAKRLEMPVFPKDKFVD
                     AVIATVKANAAFVPPYGTGATLYVRPYMFASGPVIGVAPSDEYQFRILTTPVGPYFKG
                     GAHPITLCVSDFDRAAPHGTGHIKAGLNYAMSLHAIVTAHKNGYSENLYLDSATHTKV
                     EETGGANLIFVTKENKVITPKSESILPSITRRSLLTIAKDYLGLEVEEREVYFSEVAD
                     FAECGLCGTAAVISPVGKIVDHGKEICFPSGMQDMGQVTKKLYDTLTGIQMGRLKGPE
                     GWIRVIE"
BASE COUNT         1406 a         1511 c         1270 g         1324 t
ORIGIN      
        1 acattcgtca ttgctttcca gcggaaacgg cagatccatc gcccggtact tcggcaggca
       61 tagcttccgg aacggcagca attccagctt ttgcagattg gtaaatcgca aggcttcctc
      121 agcgattttc aaaatacact cttccccatc cgccaggccc ggcacaacaa catgacgaat
      181 ccagagtggc actcgcagtt cctccgtctg cttcaaaaaa cgcagcacca cgtcccgggt
      241 gcccccgcag tattgctgat aatcctccgc gcaggagaat ttcaggtcgc agagaacgag
      301 gtccgtattg agcaaaactg cccgcgtacc ctccggatcg ccgataccgg cagtatccag
      361 cgccgtatgg acgccctcgc gacgaagacg gcgaaacaat tccgcaacga attcccattg
      421 cagcagcggt tctcctcctg aaacggtaac gcctccgttt gccgcaaaat agggcctgta
      481 gcgcagaatc ttttccgcag cttcatcgac agaacattcc gtgccgtcgg ccgggtccca
      541 ggtatctgga ttgtggcagt aggcacagcg cagtgtgcag ccctgcagaa acacgacgca
      601 tcgcaaaccc ggcctgtcga ctgccccaaa actttggaaa gaatgaattc tgcctttcat
      661 cacatcgcct cgtggaaggt gcgggaaatt acttcgcgct gctgctcctt cgaaagtttg
      721 tggaagttca cggcgtagcc ggacacgcgg atggtcagat tcgggtaacg gtcgggatgt
      781 tcataggcct ccaaaagtgt ttcgcggttc agcacattga cgttcaggtg atgcgcgttc
      841 tgcccgaaat acccattcag gagcgaaacc aggttttcat gctgttcctc ttccctgtgg
      901 cccagtgcct gcggaatgat ggaaaacgtg ttggaaatcc catccttgca gatatcatag
      961 gaaagctttg cgacggagtt cagcgaagcg agcgcgccat tcttctcccg gttatgcatg
     1021 gggttcgcgc cgggtgcaaa gggttccccg gcttttcggc cgtccggggt cgccccggtc
     1081 ttcttcccgt agacaacgtt ggaagtgatg gtcagaatgg atagcgtatg ctctgcgctg
     1141 cggtaggtcg ggttcttctt cagtgactca taaaacagct ttacctgctc ctgtgcaatg
     1201 gaatccacgc ggtcgtcatc gtttccgtaa gccgggtatt caccgatcgt ctcaaaatcg
     1261 cggatcaatc ccgtttcatc gcggatgcat ttgaccttcg cgtaccggat cgcggaaagc
     1321 gaatccgtca ggacggacag tccggcgatg ccaaacgcca tgaagcggtg cacgtcggta
     1381 tcgtgcaatg ccatctgcgt cttctcatag gcatatttgt catgcatata gtggatgata
     1441 ttcatcgcgc tgacataggt tttcgccagc cagtcccgat aaaaagccat cccggagaca
     1501 accttgccat aatcaagaat gtctccttcg taaggagcac gcgccggagc gacctgctgg
     1561 ccgctttttt catcgcgccc gccgttcaga ctcatcagca gaagcttcgc aaggtttgcc
     1621 cttgccccga aaaattgcat gtccttcccc agccgcatcg ccgaaacaca gcaagcaatt
     1681 ccataatcgt caccgaattt cggtcgcatg atgtcgtcgt tctcatactg gatggcatcc
     1741 gtatcgcagg aaacctttgc agtaaaactc ttgaaaggct ctggcaaacg tccagaccac
     1801 aaaacggtga gattcggttc cgccgcaggg cccagattat aaagtgtgtg aagcatgcgg
     1861 aagctgctct ttgtcacaag ggttctgcca tcctccccca tgcccccgac agattcggta
     1921 atccacatcg ggtcaccggc aaacagctca ttgtattccg gcgtacgcaa atgccgggcc
     1981 atgcgcagtt tcatgacaaa gtcgtcgaag atttcctgcg cttcctgctc tgttaaaata
     2041 ccgctttcca gatcacgctc aaaatagata tccaggaaag tgctaacgcg gccaaggctc
     2101 atagcggcgc cgttctgctc cttgatcgcg cccagatagc caaagtacag ccactgaacc
     2161 gcctctttgg cattctcagc cggccttgag atatcgaatc cgtacagggc cgccatatcc
     2221 ttgagatacc ccaaaaacgt gatctgctga aaaagttctt ccaactggcg aacatgatcg
     2281 gcatccatgt tctgcgcacc cacttccgcc ttatcctttt tcttctcttc gatcagccgg
     2341 tcaatcccgt aaagcgcgac ccgacggtaa tctccgatga ttcgccctct tccgtaggcg
     2401 tccggcaatc ccgtgatgat tccgcagtgg cgtgctgccc taatttcatc tgtataagcc
     2461 ctgaaaactc cctcattatg agtcgtacgg tattggaatt ccttttctac ctggtctgag
     2521 agccggtacc cgtacgcttc gcatgccttg cgcgtcatgt tcagaccgcc gaagggattc
     2581 actccgcgct ttaagggacg gtccgtctgc agccctacaa taatctcatg ctctttgtct
     2641 aggtatccgg ggcggtaact cgtcagtgag ctgacggttt cggtgtccac gtccaaaact
     2701 ccaccgaatt cctgttccag ttccaacagc cgattcagct ttttcatcag cgcttttgtc
     2761 cgctccgtcg ccggagtaag aaattccccg tctccctcat acggcgtgta attggtttga
     2821 atgaaattcc gaacgttgat ctctgtttcc caggttccgc tcttaaaatt cttccaggcc
     2881 tgattgctca taaaaaacgc tccttctgtt ttcatagcaa tccggccttg cgtcccgccc
     2941 taagtataca aaaaaggcgg cacccggcaa aaaccggaat gctgcccaga cggtcggctt
     3001 tttctaagac ttttatttcc ctgtggttta tattccacaa tcatccgtca gtaataaaag
     3061 cctgttcaat tgtattttta ttataataga caaaacccgc gcctgtcaac cggaaatcaa
     3121 aatttctctt ttcaatttta ggttaaagat gaataagcat cactaagcgg cttcttcttt
     3181 tgatgacgca acagaagaga aagaagcccg tatcttctct ccgaaaaggt tcaacaggga
     3241 accggacagg acaaacacaa cccccaaaat aatattcagc gtaatcggct cctttaaaat
     3301 caaaaaagca ataatcggtg caaaaacggg tttaataaaa aaggtaatcg aagcggtgga
     3361 gggacccgag acctcgatgg cccggatata gcaaaaataa ccgaacccag ttaccagaac
     3421 gcccaaataa agaagcagag gcagcgtatt gggctgaatt cctttcatga cagggccttt
     3481 ctttgcgagc tgaatcacaa gcaataccag cgagcccata ataaaactga agctgctctg
     3541 cgtcaaacca ccgatttttg ctatgcgctt cttccccatc gcggtgtaaa ggccaaagga
     3601 cgcggcggcg atcaaggtta ttaaaatgcc gtccacagca gtatggccct tcataagctt
     3661 gattggattt gctacgatga tcagccccat aacattcagc agcagaacga agaacttgac
     3721 cagggtaaac ttttcatttg caaaaaactg agcgaaaatc atggtgaata ccggactggt
     3781 tgaaataact acagcagcca gattcgcgtt tgtgcgaaga acaccgattt gaaacaggag
     3841 catactgacg cagatacagg taaaaccgag aagaaaaaga tacagccaat cgcttttggt
     3901 taaatgacac tgccgctttt tcaaatcgta cagtgcaaat ggaaagaggc aaagcccgcc
     3961 gatcaggaag cgcagaaagg tcatctgaat aggatcaaag gaagctcccg ccaatttgag
     4021 tgaaacctcc attgtgccga acagaaatgc agccgccaaa atacatacga tacttttttt
     4081 cattccggat ctcttttccc attttctatg tagaatacgg cgtttatcca cagatggagc
     4141 aaaaccgcag tcaacgcttt ctattatacc accttgcaat tatgattcat aacgcatatt
     4201 gtacaaagct attataaaat atgcttgaat ttgacagcaa agtggcaatt attgccacaa
     4261 atgcacacaa aaaggcaccc ctaagggtgc ctaacgaact gagaattatt cgattaccct
     4321 gatccagcct tccggccctt tgagccgacc catctggatg cccgtcaatg tatcatacag
     4381 cttctttgtt acctggccca tatcctgcat tccgctcggg aagcagatct ccttcccgtg
     4441 gtctacgatc tttccgaccg gggaaataac ggcagccgta ccgcagagac cgcattcggc
     4501 aaagtcagcg acctcgctaa agtacacttc ccgctcctca acttcaaggc caagataatc
     4561 cttggcaatg gtcagaagcg aacgacgggt gatcgatggc aggatgcttt cggatttagg
     4621 agtaataacc ttattttctt ttgtgacaaa gatcaaattt gctccgccgg tttcttccac
     4681 cttggtgtgg gtcgccgaat ccagatacag attctcactg tagccgtttt tatgggcggt
     4741 tacaatcgcg tgcaggctca tagcgtaatt aaggcccgcc ttgatatgac ccgtgccatg
     4801 cggtgccgcg cggtcaaaat cactgacaca gagcgtaatc ggatgagcgc cgcccttgaa
     4861 ataaggtccc acaggagtag tgagaatgcg gaactgatat tcatcggaag gagcaacgcc
     4921 gattaccggg ccactggcaa acatgtaggg gcgaacgtat agggttgcgc ccgtcccgta
     4981 aggcggaaca aaagcggcgt ttgcctttac ggttgcaata accgcgtcga cgaatttgtc
     5041 tttcggaaac accggcattt ccagacgctt cgcgctgttt tccatgcgct ctgcgttcat
     5101 gtccgggcgg aacgccacaa tacggccgtc ttccgtcgta tacgccttaa ggccttcaaa
     5161 gcaggactgg gaatactgga gaacaccggc gcattcgctg ataaccactt tatcatcgtc
     5221 cgtcagctgg ccctcgtccc aagcaccttc acggtaattc gcaacgaatc tcttatctgt
     5281 cttgcgatag ctgaaatcaa gattcgccca atcaagatct ttcttttcca aaataaacac
     5341 cccttcgtaa aaatttgcag cccatttaga aacaatctat cttgtgtcag cggaaaataa
     5401 aaagccctaa gctaatagct tagggcgaat ataaaatccg tggtaccacc taattcagag
     5461 aaaactctgc actcatacag taataaaata ctgcgtccgt tttaacgggc g
//
LOCUS       DATALY010000032         4596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_266264,
            whole genome shotgun sequence.
ACCESSION   DATALY010000032 DATALY010000000
VERSION     DATALY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266264"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VHR42_01120"
     CDS             complement(<1..208)
                     /locus_tag="VHR42_01120"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX3025824.1"
                     /translation="MEKSPRKRKKTVADINLIIPHQANVRILKSAARKMHICEDKFYI
                     NIDHTANTSSATIPIALDEAVGSGR"
     gene            complement(162..893)
                     /locus_tag="VHR42_01125"
     CDS             complement(162..893)
                     /locus_tag="VHR42_01125"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX3025825.1"
                     /translation="MGIEIKSTGSCLPERIVTNDDLSTFLDTSDEWITTRTGIKTRHI
                     SVGETTSDMASRAAEVALERSGLSAQQIDLVICATVTPDVCVPMVASNVKKALGVEHA
                     AAFDLNANCSSFIYAVTTACSLMESAGYRNAVVVGSDTNSQIVDWSDRATCVLFGDGA
                     GAVVLSRSERKGILSSFLGGAIDRDNVLLCRNSRDRTPFDLTHEEQKPKVEMSGQGVM
                     RFAINAFVEAVGEVTAKAQKNSRRH"
     gene            1205..2218
                     /locus_tag="VHR42_01130"
     CDS             1205..2218
                     /locus_tag="VHR42_01130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3025826.1"
                     /translation="MLNFEEKAYLADFSKVTGLKTKIESYVDEITAGGIENIFLVGIG
                     GTISLMYPMESFIKKHSKIPVYTENAAEIVLGVNCSLSEKSAVILYSESGTTKETVAA
                     AEYCKSRGIPTIGVSGADNSPLANALKYPIVSSEGDYYSSDADYIRLYMIVSRLLYRL
                     GDYPAYDAFLKALGDLPKALCDVKVKVDPAALKFATEYKDEPYHMLVGSGNMWGETYC
                     YAMCILEEMQWIHTKAIHAAEFFHGTLELVDDKTSVILLKGEDQTRPLTDRVEKFVTR
                     YTEKVTVFDTKDYALEGIPTQFRADFSPIVMTAILGRVSAHLEVVRNHSLALRRYYRK
                     VEY"
     gene            2270..2944
                     /locus_tag="VHR42_01135"
     CDS             2270..2944
                     /locus_tag="VHR42_01135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3025827.1"
                     /translation="MDSDLLKPYHLLKKSDLKKAADVFAGAFLDDPLYQCIQADRSKR
                     AACMKYFFRYYLRLMYQSSYILTDSSDMKMLAVVWDPSHSEHHPVYLAKVVWSFLRGL
                     RVIPTIGLSGYVRAVSILTRMTSAWTDQYLSRPFLHLDMFAVQKQFRGQNLGGNFLRA
                     FTGYCKERGLLCTLETQQESNIGLYHHFGFCVTEKMIDTRTRLTEYCMVCEPDSNKIQ
                     SLEKQV"
     gene            complement(3028..>4596)
                     /locus_tag="VHR42_01140"
     CDS             complement(3028..>4596)
                     /locus_tag="VHR42_01140"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3025828.1"
                     /translation="GKMEADIILLSDSIFMARNGEAHANPGAIAIRGNRIIKVGDRVT
                     VNSFKGGGTKVYDLGARTVCPGFIDNHQFFTGFALKRWGPDVTGARAENALEILKKYA
                     SGLSPERTVVGHGLSVEGWSEKEKSGQMLDREFGSRPVAVFTPDRDFCWMNRAAENKY
                     GFSPDECYAEATWKLLRELLESEEEITAQYASYQTMLASRGVTAAKEIGFDDYSGFTQ
                     ILAKLENEHKLTHRVNLVSQPVARAADFDYLTRCREKFTGEFVRCMGLNIMIDGEIDS
                     FEGDLLAPYASKPELRCGMKVDYQAVEKTVLRADREGFRVALHAEGDAAVKRCVDIFE
                     ACVRQNGKRDSRHAIVDLEIADPDDLKRMAALDVTAINYLQIMNCYPSYGAFHPEEYF
                     SPDKIKNVWQYRRMIDSGVNICCGTDLPLDEPDIPLSAYFAEARLFPDGSPRGGFNKE
                     QAITTAEVLRSWTSAGQYVNFEEKRLGMLEEGMLADIAVLDCDLFALPIERLREAKVC
                     LTLSDGRVVYDTVS"
BASE COUNT         1113 a         1262 c         1079 g         1142 t
ORIGIN      
        1 cccggccgct cccgaccgct tcgtcaagag caatcggaat ggttgccgag gaggtgttcg
       61 ccgtatgatc aatgttgata tagaatttat cttcgcagat gtgcattttt cgggcggctg
      121 attttagaat tcggacgttg gcctgatgcg gaatgatcag attaatgtcg gcgactgttt
      181 ttttgcgctt tcgcggtgac ttctccaacg gcttctacaa aagcgttaat cgcaaagcgc
      241 attacgccct gaccgctcat ttcgactttg ggtttctgct cctcatgcgt caaatcgaat
      301 ggagtccgat ctctcgagtt acggcagagc agaacattgt cccggtcaat cgccccgcct
      361 aaaaagctcg acaggatccc cttcctctcg gatctggaga ggactaccgc tcccgccccg
      421 tcgccgaaca gcacacaggt ggcccggtcg ctccaatcaa caatctggct gttggtgtcg
      481 ctgccaacca caaccgcgtt gcggtaaccg gcgctttcca tcagactgca ggccgtcgtc
      541 acggcataaa taaaactgga acagttggcg ttcaggtcga atgccgctgc atgttccaca
      601 cccaatgcct ttttgacgtt agaggccacc atcggcacac agacgtccgg ggtaacggtg
      661 gcgcagatca ccagatcaat ctgctgagcg gaaaggccgc tgcgttcgag cgcaacctcc
      721 gccgcgcggc ttgccatgtc cgacgtcgtt tctccgacag aaatatgcct tgttttaata
      781 cccgtccggg ttgtgatcca ttcatcgctt gtatctaaaa atgtcgataa atcatcgttt
      841 gttacgatcc gctccggaag gcagctgccg gtcgatttta tttcaattcc cattctaatc
      901 ctcttttatt gtcataatcc agcttctggg ccgggttgtt tgatgttaaa ctttattata
      961 atactttcca ctgcgcttgt aaaacaacca tctgaaaatc ttatagcaaa tttgctcctt
     1021 atgacgatcg ggcaatgacc tgcaatcatc cgaatttttc ggctcttgac agttcggcaa
     1081 aatattggta tgcttatatt gctaagtggt atgtaaatca taccaatcgg atttaagaaa
     1141 gctttaccga ttctgatcag accactttcc aacaatcttc atcatcaagg aggcaaacga
     1201 aaccatgcta aattttgagg aaaaagcata tcttgcggat ttttccaagg taaccgggct
     1261 gaaaacaaaa atcgaatcct atgtggatga aatcacagcc ggcggtattg aaaacatttt
     1321 tttggttggc atcggcggaa ccatttcgct gatgtaccca atggaaagtt tcatcaaaaa
     1381 acattccaaa attccggtct ataccgagaa cgccgcggag attgtccttg gcgtcaactg
     1441 cagtctgagc gaaaaatcgg cggtcatcct ctattccgaa tcgggcacaa ccaaagaaac
     1501 ggttgccgct gccgaatact gcaaatcacg tgggataccc acaatcggtg tatcgggagc
     1561 agacaattcc ccgctggcca acgccctgaa atacccgatc gtctccagtg aaggcgatta
     1621 ttattcttcc gacgccgact acatccgcct ttatatgatt gtttcacggc ttctttacag
     1681 gctgggtgat tatcccgcat acgacgcgtt tctgaaagcg ctcggcgatc tgccaaaagc
     1741 gctgtgcgac gtaaaggtca aggtagatcc ggcagctttg aaattcgcca ctgaatacaa
     1801 agatgagcct taccacatgc tggtcggttc gggcaacatg tggggcgaga cttattgcta
     1861 cgcgatgtgt attctcgaag aaatgcagtg gattcacacc aaagcgattc atgccgccga
     1921 atttttccac ggcacgctgg agcttgtgga cgataaaacc agcgtgattc ttcttaaagg
     1981 ggaagaccag acccggcccc tgaccgaccg cgtcgaaaaa tttgtcacaa gatacaccga
     2041 aaaagtgacg gtttttgaca cgaaggacta tgcccttgaa ggcattccaa ctcagttccg
     2101 ggccgatttt tcccccattg tcatgacggc gattctgggt cgcgtcagcg cccatctgga
     2161 agtcgtccgg aatcattcac tggcgctgcg aagatattac cgcaaagtgg aatattgagg
     2221 ccgggatagc ggccccaccc accaaaaacc aaagattaga aggggaagaa tggattcaga
     2281 cttattgaaa ccctatcacc tgctaaaaaa atcggatctt aagaaagcgg ccgacgtatt
     2341 tgccggcgct tttctcgacg acccgctgta tcaatgcata caggccgacc gcagcaaacg
     2401 ggcggcctgc atgaagtatt tttttcggta ttacctccgt ctgatgtatc agagcagcta
     2461 tattctgacc gattccagcg atatgaaaat gctcgccgta gtttgggacc caagccattc
     2521 cgaacatcat ccggtttatc tggccaaggt agtttggagc ttcctcaggg gacttcgtgt
     2581 aattccgact atcgggctga gcggatatgt ccgtgccgtt tcaatcctta cgcgtatgac
     2641 ctccgcctgg acggatcaat atctctcccg gccgtttttg catttagaca tgtttgctgt
     2701 tcagaagcaa ttccgcggac agaatctggg cggaaacttt ctgcgggcct tcaccggcta
     2761 ctgcaaagag cgcggccttc tctgcacact ggaaacccag caggaatcca acatcggcct
     2821 ttatcatcac tttggttttt gtgtaacgga aaagatgatt gatacccgga cgcgccttac
     2881 cgaatactgc atggtatgcg agccggattc aaataaaatt cagagtcttg aaaaacaggt
     2941 ttaactgctt acccgcctcc taaagaaaga gctccggcga agaaatcatt tccttcgccg
     3001 gagctctttc ttgcataccc ttccgtttca agacaccgta tcgtagacca cgcggccgtc
     3061 cgacagggtc aggcagactt ttgcttctct gagtctttcg atgggaagag caaacaaatc
     3121 gcaatccagc accgcgatat cggcaagcat cccttcttcg agcatgccaa gccttttttc
     3181 ctcaaaattg acatactgcc cggcggaagt ccagctccgg agcacttcgg ccgtggtaat
     3241 cgcctgctcc ttgttgaagc ctcccctggg agagccatcc gggaataagc gggcctcggc
     3301 aaaataggca gagagcggaa tatccggttc atccagagga agatccgtgc cacagcaaat
     3361 attgactccg ctgtcgatca tccgacggta ttgccagacg tttttaattt tatccggcga
     3421 aaagtattcc tccgggtgaa acgctccata actgggatag caattcatga tctgcaaata
     3481 gttgatcgct gtgacatcca gcgcggccat ccgttttaaa tcgtccggat cggcgatctc
     3541 cagatccact atggcgtggc gagagtctct tttgccgttt tgtctgacgc acgcttcaaa
     3601 aatatcaacg caccgtttga ccgccgcgtc gccctccgca tggagcgcca cccggaatcc
     3661 ttcccggtcg gccctcagaa cagttttctc aacggcttga taatccacct tcataccgca
     3721 ccgcagttcc ggtttggagg catacggagc cagcagatcg ccctcaaagc tgtcaatttc
     3781 tccgtcgatc atgatattca ggcccataca gcggacaaac tctccggtaa atttctcgcg
     3841 gcaccgggtg agataatcaa aatccgcggc ccgtgcaacc ggctgagaaa caagattgac
     3901 cctgtgtgtc agcttatgct cattctccag cttcgcaaga atctgagtaa atccagaata
     3961 atcgtcgaaa ccgatttctt tggcggccgt tacccccctg gacgccagca tcgtctgata
     4021 actggcatat tgggccgtta tctcttcctc agactccaaa agctcccgga gcagtttcca
     4081 tgttgcctcg gcgtagcatt catccggcga aaaaccatat ttattctcag cggcgcggtt
     4141 catccagcaa aagtcacgat ctggtgtaaa aaccgcaacc ggacggctgc caaattcccg
     4201 gtcaagcatt tgtccgcttt tctctttttc gctccagcct tccaccgaca ggccgtggcc
     4261 gacaaccgtc cgctccggtg agagaccgga agcatatttt ttcagtatct caagagcgtt
     4321 ttccgcacgc gcgccggtca catccggtcc ccaccttttg agcgcgaatc cggtgaaaaa
     4381 ctgatggttg tcaataaatc ccgggcaaac cgtcctggcg cccaagtcat agacttttgt
     4441 cccgccacct ttgaaagaat tgaccgtcac ccggtctccg accttaataa ttctgttccc
     4501 ccgaatggca atggccccgg gattcgcatg ggcttcgcca ttgcgggcca tgaaaatgct
     4561 gtcagacagc agaatgatgt ccgcttccat tttacc
//
LOCUS       DATALY010000033         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_525074,
            whole genome shotgun sequence.
ACCESSION   DATALY010000033 DATALY010000000
VERSION     DATALY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525074"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VHR42_01145"
     CDS             complement(<1..306)
                     /locus_tag="VHR42_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX3025829.1"
                     /translation="MLSILRIILIPVFILLFFSNGTKSVLIAAIVLIVSGLTDILDGI
                     IARKFNMTTALGRILDPLADKLTQASVCICLVVKKVAPVWLLLLLILKEVLMIAGGAK
                     "
     gene            complement(486..1187)
                     /locus_tag="VHR42_01150"
     CDS             complement(486..1187)
                     /locus_tag="VHR42_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX3025830.1"
                     /translation="MSLFLDVVTVAVMGATILFSYRKGLVRSVIELAGYIAAAVAAYI
                     FSVPLGNWLFSLFRPMLAGKFSAGISQALAQQSGGVGTGLNEIAKFVSKYGISTGNAS
                     AWSAGLKSGETALTGSVMNSVVDPFASGIGRILAFALIFALCMVLVRLVARASDVVFH
                     IPVIRQVNGFGGAVVGVVKGFVIMFLISAIITVLIPVFALEKNPPITSQTVSSTIVYK
                     YICQINPLTQLLLKK"
     gene            complement(1260..2516)
                     /locus_tag="VHR42_01155"
     CDS             complement(1260..2516)
                     /locus_tag="VHR42_01155"
                     /inference="COORDINATES: protein motif:HMM:NF039834.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HEX3025831.1"
                     /translation="MKNKDGGSPDSQTEKSRIVTIRFDGKNDRNKDETAIVVKDGAAK
                     RTKKKKPKKRRMILLALALLAVAFIYLNWDALSPQSIGQTFGDVFSGFEKSRYPVMLS
                     QGNFIGAVPVGQNLGVLTDTSFLIYSSTGKPLAERQHGMSSPSVISSGNKAMIYDRGG
                     KQYKVETRYSEVYSGQTDYPIITAAVSKSGYFALVTESDNYLSEILVYNQNGVNVFKY
                     YSAEGRVLSVALSPDGSRIAAVTVGAKNGDIQSTVKIYKTSGRNEISSKSYDGLLLFS
                     IGFKGNDRITAIGDTASIFLHNDGSSISRYDYQDKTLKCYSNTPWTTALVFSKYGVGS
                     DSTVTSFGANGDVAGQTDIHSDVKSVYAGNKMVVALTQRGIWHSDTSLKNSATTNVSG
                     DMIHTISIQNDVYIFGLQAVYQYKES"
     gene            complement(2521..3261)
                     /gene="truA"
                     /locus_tag="VHR42_01160"
     CDS             complement(2521..3261)
                     /gene="truA"
                     /locus_tag="VHR42_01160"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX3025832.1"
                     /translation="MRNILVFITFNGARYHGWQVQKNAVTVQQTVQDALEKILKTRPG
                     IVGCSRTDAGVHAYEYAFQFRTQSTIPCISLVKALNVTLPHDIAAFRCREVNEDFHAR
                     YSVVYKEYIYQIYNSKTRNPFFEGRALFVPRELDCAAMADAAARFLGRHDFTSFCAAG
                     GSVLDKVRNIMLFDVAREGSLVTIRIRADGFLYNMVRILAGTLLDVSDGKIGAGDIKA
                     ILASGDRNKAGPTLPAHGLYLNQVCYGE"
     gene            complement(3297..4112)
                     /locus_tag="VHR42_01165"
     CDS             complement(3297..4112)
                     /locus_tag="VHR42_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX3025833.1"
                     /translation="MIKDITLGQYFPGESAVHKLDPRMKIVLTCVYMVVLFCAFNAWG
                     LVLSGLFMVAVILISRIPVRLVFKSVKPIVPVIIITSLINAFYIPGRSIFKFYFMNIT
                     LEGVEMAVFMSVRIVFLIVGTSMMTYTTSPISLTDGLERLMAPLKAMKLPVHELSMMM
                     TIALRFIPTLIEETDKIMSAQKARGADFESGNLMRRMKALLPILIPLFVSAFRRAEEL
                     ALAMDCRCYKGGEGRTRMRQLRMTSYDYSAAGLFTLLLGGVIALNLLAPSLIK"
     gene            complement(4109..>4453)
                     /locus_tag="VHR42_01170"
     CDS             complement(4109..>4453)
                     /locus_tag="VHR42_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEX3025834.1"
                     /translation="GRDSILARIREYHQEKHSTILLVSHSMEDIAENATRVLVMNRAR
                     VAMYGSVDEIFARSGELIAMGLDIPEVTRIFIGLRRKGFDLSDAVYTVEQGKREILRY
                     LGRESENGGNGR"
BASE COUNT         1162 a         1220 c          992 g         1079 t
ORIGIN      
        1 tttggctcca ccggcgatca tcagaacctc tttgagaatc agaagcagaa gcagccagac
       61 cggagcaacc ttttttacaa ccagacaaat gcagactgac gcttgggtca gcttgtcggc
      121 aagcggatcg aggatgcgtc ctaaagccgt cgtcatgttg aattttctgg ctataattcc
      181 atccaatata tctgtaaggc ctgacacaat cagaacaatt gccgcaatca ggacagattt
      241 tgtaccatta gaaaaaaaca acaggataaa aaccgggatc aagatgattc tcaatatgga
      301 aagcatattg ggaattccca gattttttta tgctgcgggt tcgaataccc gacatacaca
      361 aaacgccccc tcgtgcaaat tgaagtccgg atctcagaca ttttgtttga catagttttc
      421 tatcactata gattggattg tctcatctgt taaaaatgca aaagcactat aataatatca
      481 tattactatt tttttaacaa cagttgcgtt aagggattta tctgacaaat atatttgtag
      541 actatcgtac tgctgactgt ctgactggtt atggggggat ttttttcaag agcaaatacg
      601 ggaattaata ccgttattat ggcgcttatc aggaacatta ttacaaaacc tttcacaacg
      661 ccgacgacag cgccgccaaa accgtttacc tgacgtatca ccggaatatg aaaaacaaca
      721 tcggatgctc tcgcgaccag ccgcaccaaa accatgcaaa gagcaaaaat cagcgcgaac
      781 gcaagaatcc tgccgatgcc ggaagcgaat ggatcaacca cagaattcat caccgatccg
      841 gtcagagcgg tctctccgga ttttaatccg gcagaccatg ccgaggcgtt ccccgtgctg
      901 ataccatact ttgaaacaaa ctttgcgatc tcattcagcc ccgtcccaac cccgccgctt
      961 tgctgcgcca aggcctggga gatgcccgcg gaaaacttcc cggccagcat cggccgaaaa
     1021 agagaaaaaa gccagttgcc caaaggaacg ctgaaaatat aagcggccac cgccgccgca
     1081 atatagcccg caagctcaat taccgagcgc accagccctt tccggtaaga gaacaggatt
     1141 gtcgcgccca taacagccac cgttaccacg tctaagaaaa ggctcatttt gacatctcct
     1201 tatcagaaac tcccgttttt ttattttcga ctgttaagcc ggcatgttct gccgtccgct
     1261 caactttcct tatactgata tactgcctga aggccgaaaa tatacacgtc attctgaatg
     1321 gatatcgtat ggatcatatc tccggaaaca tttgtcgtcg ccgaattttt taaactcgtg
     1381 tcgctgtgcc agattccgcg ctgggtcagc gcaacaacca ttttgttccc cgcgtaaacc
     1441 gatttgacgt cagaatgaat atcggtctgg ccggcaacat ctccattggc tccgaacgac
     1501 gtcaccgtgg agtcgctccc cacgccgtat ttgctgaata ccagcgcggt ggtccatgga
     1561 gtattgctgt aacatttcag cgttttatcc tgataatcat agcgggagat gctcgagccg
     1621 tcgttatgta aaaaaatgct ggccgtatcg cctatcgccg tgatcctgtc attgccttta
     1681 aacccgattg agaaaagcag cagcccgtcg tagcttttgg aagagatctc atttctgccg
     1741 ctcgtcttat aaattttaac cgtcgactgg atgtccccgt ttttcgctcc gaccgtaacg
     1801 gcagctatcc ggcttccgtc cggactgagt gcaacgctca ggacacgccc ttccgccgaa
     1861 taatacttaa aaacattcac gccgttttga ttgtaaacca gaatttcact caggtaattg
     1921 tccgattcgg tgacaagggc gaaatatccg cttttgctga cggccgccgt tataatcgga
     1981 taatcggtct gacccgaata gacctcggaa taccgggttt caactttata ctgcttaccg
     2041 cccctgtcat aaatcatggc tttgtttccg gaagaaataa cggaggggct gctcattccg
     2101 tgctgacgtt cggccagcgg ctttcctgtc gatgagtaaa tcaggaagga tgtgtcggtc
     2161 agaacaccaa gattctgccc aaccggaacc gcgccgataa aatttccctg ggagagcatg
     2221 acgggataac ggcttttttc gaagccgctg aacacatcgc cgaacgtctg gccgatcgac
     2281 tgcggcgaga gggcgtccca gtttaaatag atgaatgcca cggccagaag ggccagagcc
     2341 agaagaatca tccggcgttt tttaggtttt ttctttttgg tacgcttcgc cgcgccatct
     2401 ttaaccacaa tggcggtttc atccttgttc ctgtcgtttt ttccgtcaaa ccgaatcgta
     2461 acgatcctgc tcttttccgt ttgactgtcc ggcgatccgc cgtctttatt cttcacaaaa
     2521 tcactccccg taacagacct gattcagata taacccgtgg gctggaagag tgggaccggc
     2581 cttgttccgg tcacctgaag caaggatcgc tttgatgtct ccggctccga tttttccatc
     2641 ggaaacatcc aaaagcgttc cggccagaat ccggaccata ttatataaaa agccatcggc
     2701 tcgaatcctt atggtaacaa ggctgccttc ccgggcaacg tcaaaaagca tgatgtttcg
     2761 aaccttatcg agaacgctcc cgcccgccgc gcagaatgat gtgaaatcat gtctgcccaa
     2821 aaagcgcgcg gccgcatccg ccatggctgc gcaatcaagt tctctgggaa caaacagggc
     2881 ccttccctca aaaaaggggt tacgggtctt actgttatag atctgataaa tatattcctt
     2941 ataaaccacg ctgtatctcg catggaaatc ttcgttaacc tctctgcatc ggaacgcggc
     3001 gatatcgtgc ggcagagtca cattcagcgc cttcacaagg cttatgcagg gaatcgtgga
     3061 ctgcgtgcgg aattggaacg cgtattcata agcgtgaacg cccgcgtccg tccggctgca
     3121 tcccacaatt cccggccgtg ttttcagaat tttctccagc gcgtcctgca cggtctgctg
     3181 aacggtcacg gcgtttttct gtacctgcca tccatgataa cgcgcgccgt taaatgtaat
     3241 aaacaccaga atatttctca tcctgccacc ccgttcgaca tcccgtattt cagctgtcat
     3301 tttatcaatg acggcgccaa aagattcagg gcaataaccc ccccgagcag caaagtgaac
     3361 aaaccggcgg cggaatagtc gtaagacgtc atcctgagct gccgcatccg ggttcttccc
     3421 tccccgcctt tataacaccg gcaatccatt gccagcgcaa gctcctccgc gcggcggaac
     3481 gctgaaacaa acagcggaat caagatcggc agcagcgctt tcatccgcct catcaaattg
     3541 cccgactcaa agtcggcccc cctggctttc tgcgcgctca tgattttatc cgtttcctca
     3601 atcagagtgg ggataaaccg cagggcgatc gtcatcatca tcgacagttc atgcaccggc
     3661 agcttcatcg ccttaagcgg tgccatcaga cgctcaagac cgtcggtgag cgaaataggc
     3721 gaagtggtgt aggtcatcat cgaggttccc acaatcagga aaacgatgcg caccgacatg
     3781 aaaacagcca tttccacgcc ctcgagcgtg atattcataa agtagaattt aaaaattgat
     3841 ctgcccggta tataaaaagc gttaatcaga gacgtaataa taattaccgg aacaataggc
     3901 ttgacacttt taaaaaccag cctgaccgga atgcgtgaaa tcaggatcac agccaccata
     3961 aacaggccgg aaagcacaag gccccatgcg ttaaaagcgc agaacagcac caccatatat
     4021 acgcaggtca gcacgatttt cattctcggg tcaagcttgt gaacggccga ttcacccgga
     4081 aaatactggc ccagggttat gtctttaatc atcggccatt ccctccgttt tcactttcac
     4141 gaccgagata gcgcaaaatt tcgcgtttgc cctgctccac cgtataaacc gcgtcgctta
     4201 agtcaaaacc tttccggcgc agcccgatga aaatgcgggt aacctccgga atatccagcc
     4261 ccatcgctat gagttccccg ctgcgggcaa agatttcatc aacgctgcca tacatggcga
     4321 cccgggcccg gttcatcacc agaaccctgg ttgcgttttc agcaatatcc tccatgctgt
     4381 gggaaaccag cagaatggtg gagtgcttct cctgatgata ttcccgaatt ctcgcaagaa
     4441 tggaatccct tcc
//
LOCUS       DATALY010000034         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_439576,
            whole genome shotgun sequence.
ACCESSION   DATALY010000034 DATALY010000000
VERSION     DATALY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439576"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VHR42_01175"
     CDS             <1..986
                     /locus_tag="VHR42_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3025835.1"
                     /translation="RFFEQELIQMDVEKNLPLSIIMGDVNGLKLTNDAFGHLAGDSLL
                     KAAASVMRKSCGKDDIVARWGGDEYIILLPNTDNREAGKIAEHIRATCAETTVGNISL
                     SISLGYSTKARAQDDLMEALKRADDSMYKSKLIEGRSMKSGAVNTIINTLHEKNPREK
                     NHSLRVGAICRQIGLAMGFLQSQITDMELLARIHDIGKISIDEKVFEKTGKLTREELE
                     EIHRHPETGYYIIGASPELSYLANDVLSHHERWDSGGYPNHLAGNNIPLLARILSIAD
                     AYEAMTGERQYKKAMTKEEAMEELKRCSGSQFDPVITEKFLRVLKDGNCLL"
     gene            1111..1644
                     /locus_tag="VHR42_01180"
     CDS             1111..1644
                     /locus_tag="VHR42_01180"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3025836.1"
                     /translation="MEFIKKEYNVQVLERAIKTIEALSKHPSGIELRFLADELALNKT
                     TVFRILATLLPYDYVRQDPYTGKYRLGYKFIGLSNTVMQNIDIHEVAMPFLDELSRLT
                     GEVVHLVIADHDMGIYIAKVDKSSGSVKMVSNIGSHFSLRPILAGAKQTLRCSARTAR
                     ADLSDSPACGRIKIRVA"
     gene            1714..2037
                     /gene="trxA"
                     /locus_tag="VHR42_01185"
     CDS             1714..2037
                     /gene="trxA"
                     /locus_tag="VHR42_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX3025837.1"
                     /translation="MPVINLTRNNFEEIVMNSDKPVLIDFWAGWCGPCRMQSPIIEEL
                     AGELGDSAVIAKINVDESPELAARFSVMSIPTLVFLKDGEIVSRKIGVTSKPALLSIL
                     GSVRA"
     gene            complement(2125..2784)
                     /locus_tag="VHR42_01190"
     CDS             complement(2125..2784)
                     /locus_tag="VHR42_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEX3025838.1"
                     /translation="MKKFNDILKQDWYVLIIILAGFIIGAVFYPRLPERVPIHWDMSG
                     NVNGYGSKMTAAFLLPLINLGVYLLMLVSPKIDPRAKNYEKFKGSYQIIKCIPIAILM
                     IAQISSLLFAVGFKVNIAMVSQIALSLMFIIIGNVMGRFRHNYFVGIRTPWTLANEEV
                     WRKTHRFSGPVWVLGGLADLVLTLAAVRYQTIGFVIILAVMVIVPVVYSYLVFRRINS
                     R"
BASE COUNT          833 a          630 c          729 g          715 t
ORIGIN      
        1 gccgcttttt tgagcaggag ctgattcaga tggatgtgga aaagaatctt ccgctgtcga
       61 tcatcatggg cgacgtgaac gggctgaaac tgaccaacga cgcttttgga catctggccg
      121 gcgacagcct cctcaaagcc gccgcctccg tcatgcggaa atcctgcgga aaggatgata
      181 tcgttgcgcg ctggggcggg gatgaataca tcattctttt gccgaatacc gataacagag
      241 aagctggaaa gattgccgaa cacatccgtg cgacctgcgc ggaaaccacg gtcggcaaca
      301 tttcactttc catatccctt ggctattcca ctaaggcaag ggctcaggat gatttgatgg
      361 aggcgctcaa gcgggctgac gattctatgt ataaaagcaa gctgatcgag ggccgaagca
      421 tgaaaagcgg agcggtcaat acaattatca atacgcttca tgagaagaat cccagggaga
      481 agaaccattc cctgagggtc ggagcaatct gtcggcagat cggactggcg atgggttttc
      541 tgcagagtca gatcacggat atggaactgc ttgccagaat acacgatatt gggaaaatat
      601 ccattgacga aaaggtgttt gaaaaaacgg gcaaattaac ccgggaagag ttagaagaga
      661 tccaccgcca tccggaaaca ggatattata ttatcggggc ttccccggaa ctatcctatc
      721 ttgccaacga tgttctgtcc catcatgaga gatgggacag cggagggtat ccgaatcatc
      781 tggctggaaa caatattcca ctgttggccc ggatattaag cattgcggac gcatatgaag
      841 ctatgacggg ggaacgtcaa tacaaaaagg cgatgaccaa agaggaagcg atggaagaat
      901 tgaaacgatg ctccggcagt cagtttgacc cggtgattac agagaaattt ctaagggttt
      961 taaaggacgg caactgctta ctctgatggc aagtctatga agtgattgtt atattttaca
     1021 gaaacaatac gtgaagttta tttacatatt tttacagaag tgtttataat gtaaaaacac
     1081 cgttcatata atgaaatagt ctggaggata atggaattca taaaaaaaga atataacgtt
     1141 caggtattgg aacgcgcgat taaaacgatt gaagcgcttt ccaaacatcc ttccggaatc
     1201 gaattaagat ttctggctga cgaattagcg ctgaataaaa caacggtttt tcgaattctt
     1261 gctacccttt tgccctatga ttacgtccga caggatcctt ataccgggaa atacaggctc
     1321 ggttataagt ttatcggttt gtcgaacacg gtaatgcaga atattgatat ccacgaagtg
     1381 gcaatgcctt ttcttgacga gttgtccaga ctgacgggcg aggtggtaca tctggtgatt
     1441 gccgatcacg atatgggcat ttatatcgct aaagttgata aatcttccgg tagtgtgaag
     1501 atggtttcca acattggcag tcatttttct ttgaggccta ttttggcggg agcgaagcaa
     1561 acgttgcgct gctccgctcg gacggcgcgg gcagatttat ccgatagtcc tgcatgcggc
     1621 cggataaaaa ttcgtgtagc gtgattaagt tacagaaggc caaaacggtt cggattataa
     1681 taaaggcaaa tcaaagctgg aaaggtgatt gaaatgcctg taattaatct gacccgcaat
     1741 aatttcgaag aaattgtcat gaactccgat aaacccgtgc tcatcgattt ttgggccgga
     1801 tggtgcggcc cctgccgaat gcagtcaccc attattgagg agcttgccgg agagctgggt
     1861 gattcagccg ttatcgccaa aattaatgtc gacgaaagcc ccgagctcgc cgcgcgcttt
     1921 tccgtgatga gcatcccaac gctggtgttt ctgaaggacg gcgaaattgt aagccgaaaa
     1981 atcggcgtta catcaaaacc tgctctttta tccattctgg gttctgttcg ggcttaaaac
     2041 tcactttcgc cttccatatt taaaacgaac ccggccattt tcaatggccg ggttcgttgt
     2101 tcgttcagac ctgaatcagg cctgtcagcg gctgttgatt ctgcggaaga ccaggtaaga
     2161 ataaacaacc ggaacgatga ccattacagc gagtatgata acgaaaccga tggtttgata
     2221 cctgacggcg gccagtgtaa ggacaaggtc tgccaaacct cccaaaaccc agacggggcc
     2281 tgaaaaacgg tgcgtttttc tccagacttc ctcgtttgca agcgtccacg gtgtccgaat
     2341 gccgacaaaa taattgtgcc ggaaacggcc catgacgttg ccgataataa tgaacatgag
     2401 cgacagggca atctgagaaa ccatagcgat gttgacctta aaaccaactg cgaaaagcag
     2461 ggaagaaatc tgcgcgatca taagaattgc gatcgggatg catttgatga tttgatagct
     2521 gcctttgaat ttttcataat tctttgccct gggatcaatt tttggtgaaa caagcatcag
     2581 caggtatacc ccaagattga tcaacggcag gagaaaagcg gcagtcattt tcgatccgta
     2641 accgttgacg ttccccgaca tatcccaatg aatggggacg cgttcgggca ggcggggata
     2701 aaagaccgcg ccgatgatga aaccggcaag aataatgatc agaacatacc agtcctgttt
     2761 tagaatatca ttgaattttt tcatgttgtt cgccttcctc tgataaatta gacaactcaa
     2821 aaagccagcg gatgacttcc tgaaaaaccg tagtgttcag cgagtaaaca acgttttgtc
     2881 cccgacgttc gtcaagaaca agcgatg
//
LOCUS       DATALY010000035         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_612003,
            whole genome shotgun sequence.
ACCESSION   DATALY010000035 DATALY010000000
VERSION     DATALY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612003"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VHR42_01195"
     CDS             complement(<1..259)
                     /locus_tag="VHR42_01195"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX3025839.1"
                     /translation="MAILIAVIDLKIHQIPNEMVLCVVILGSAFQLFSGGGKGLLYAG
                     LSMLAVMACFTLLAGAMGFRSVGAGDIKLAGAMGLTLSYPYI"
     gene            complement(1025..>1961)
                     /locus_tag="VHR42_01200"
     CDS             complement(1025..>1961)
                     /locus_tag="VHR42_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025840.1"
                     /translation="YANNTLIGSSAITNGMAQTNWKPSDENTVSLKAVYQPDAAKNDR
                     YGRVESTAISYTPASNPSGGSSGSSSTVSLPSSLTDAAAGVQADLFGAEFPSYVTSIS
                     LSVTKPAQGDRQAADFRTALLASPSTGVINIPELCNISLLDQSGSPVSFTGTVRMKIP
                     LPAGVRGTPHVFRCEPDTQKFTDMNATVANGYLVFSTDHLGDYAIAGVGNAIVLDTLN
                     YQMPVNGQYQIGLRLPGTKAASVKYYSTNDKVAAVIRLKNGNYQVIAKGLGTAYIMFD
                     VYDNKNHLLTHASVRIDVKTGIRPRGDSTRQFGVF"
BASE COUNT          541 a          466 c          460 g          494 t
ORIGIN      
        1 aaatataggg atagctcaag gtcagtccca ttgcacctgc aagctttata tctccagcgc
       61 cgacgctgcg gaatcccatt gcaccggcca ataaagtgaa acacgccata actgccaaca
      121 tagaaagtcc ggcatagaga aggccttttc cgccgccgct gaaaagctga aaggcagaac
      181 ctaaaatcac tacgcataaa accatttcat tgggaatctg atgaattttc agatcgatca
      241 cagcgatcag aatagccacg gtccaaagcg aactaaaaaa ggaaggtggt aaagggactg
      301 cgtgaaaaac aggaaaccac tgtccagccg gctgcattga ttatgcttgt aacgatactg
      361 aacgttaaat tcggcatcca aagcagctcc cccgcaaatc cgttcccgcc ggaatattta
      421 taagtaatta ttcgttgtgc aagctgcgga aagcagtacc ctgtgagcaa gccaaatatt
      481 ccaaggaaaa tgctgtattc tgttatcata gttttctcca aaatgttaat acgaataaat
      541 gtaaagccgg ggaaccgaaa tacgttaagg tcacttgatc tcgatgtaat gggtcgttgt
      601 tacgtcaaat tttgagttgt cattgggtag tttgaattct tgcattattt aatacaaaat
      661 cctatgaaac agcgtaaatt tttataaaag taatctctta tttattttaa atctgatacc
      721 attccacata attgcggggt tgaactttgg tggattttcc ataatattct tatcttttta
      781 acaccggcaa atggactctc cgcggccaca cacccgcaaa agatttccgg gcagcgcagt
      841 gtttcaagac aaagacctgt gtattcttct cctacaaacc gatgagccaa cgaaaacaga
      901 gtggttccac tcggtcagga gcacggagaa aactttatcc ggcaatcttg ttcacaagcg
      961 gcttcgaaca tagcaaaggt ccgctcctgt agaaaggggc ggaccctgtt tattttgctg
     1021 aatatcagaa tactccgaac tgccttgttg aatcgcccct aggccgtatt ccggtcttta
     1081 catcaatcct cacagatgca tgggtaagca aatgattttt attgtcgtac acgtcgaaca
     1141 tgatatacgc cgtgccaagc cccttggcga taacctgata atttccgttt ttcagcctga
     1201 tgaccgctgc aactttatcg ttagtagaat agtattttac acttgctgct ttcgttccgg
     1261 gcagacgcag accaatctga tattggccgt tgaccggcat ctgataattc agcgtatcca
     1321 aaacaatggc gttccccacg cccgctatgg cgtaatcgcc gagatgatcg gtggagaaga
     1381 caagataccc gtttgctact gtcgcgttca tgtcggtgaa tttttgcgtg tccggttcac
     1441 agcggaaaac atgcggcgta ccgcgaacac cagccggaag cggaattttc atccgaacag
     1501 taccggtaaa ggaaaccgga cttccactct gatcaagcag agagatatta cacagttccg
     1561 ggatattgat gacgcctgta cttgggctcg caagcaaagc agtgcggaaa tcggccgcct
     1621 gtctgtcgcc ctgcgctggt tttgtgacgg agagcgaaat tgaagtaacg tacgacggga
     1681 actccgcgcc gaaaaggtcg gcctgtaccc cggcagcagc atcggtcaaa ctggacggca
     1741 aagaaacagt ggaagagctg ccggatgaac cgccggaagg attggacgcc ggcgtatagc
     1801 tgattgccgt agattccact ctgccgtagc ggtcgttttt ggcagcatcc ggctggtaga
     1861 ctgcctttaa agaaaccgta ttttcgtccg acggcttcca gtttgtctgc gccatgccgt
     1921 tggttattgc agatgaacca atgagggtgt tattcgcgta a
//
LOCUS       DATALY010000036         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_180507,
            whole genome shotgun sequence.
ACCESSION   DATALY010000036 DATALY010000000
VERSION     DATALY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180507"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VHR42_01205"
     CDS             <1..1011
                     /locus_tag="VHR42_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3025841.1"
                     /translation="TFLRACNYYRTAGFYRGLLIADGCTEEVWENLKKLDGLSLECFS
                     KVIQYGKPKIEPIEVPYENTTLPGHFYRVPSEAEFKTAPTLVMINGYDGTKEEMYGCA
                     LEALKRGMNCFAFEGPGQGEMIRTRGIPFRPDWENVVTPVLDYLIQKLGVAPDRIILW
                     GESMGGYLAPRAAAFDHRAAACVANSGVYDFMGTRGPKGIDRNTYFGYIEQMPEADFE
                     AMMKKQMDLVVQLNWALKHGMYVFQAKSLKDYFLKAKDYYMGDICGQIACPTLVIDTE
                     GENNFPGQAKMLYDALNCPKDYLLFTEAEGAEEHCEEGSKLYANCRIFDWIENTLGGL
                     GW"
     gene            complement(1103..1591)
                     /locus_tag="VHR42_01210"
     CDS             complement(1103..1591)
                     /locus_tag="VHR42_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEX3025842.1"
                     /translation="MNFLSDDIRVLRFNGVLINGYSRKQVDEVLRQIAEENEQKYGAE
                     IREKYGVETVEQANRKLADMTEETFNRAAALEKQMIELFLEAFQTGNPASEAAQCAAD
                     LHRQWLSFYWAQYSPEAHAGVARMYVADPRFTAYYDKYQPGLAEFLRDAVLIYTGQIQ
                     PQ"
     gene            1791..>2284
                     /locus_tag="VHR42_01215"
     CDS             1791..>2284
                     /locus_tag="VHR42_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3025843.1"
                     /translation="MEYTVKKLAQLSGVSPRALRFYDEIGLLSPARINSSGYRLYGER
                     EVNLLQQILLYRELGLSLEEIKQIVTQSDFDVLAALQEHRKQLLEQRTRLDALIENVE
                     KTIRNAKGETSMTDSEKFEGFKQKLIDENEQKYGAEIREKYGVETVEQANRKLADMTE
                     ETFNR"
BASE COUNT          582 a          558 c          621 g          523 t
ORIGIN      
        1 accttcctcc gcgcctgcaa ttattaccgt actgctgggt tctaccgcgg tcttctcatt
       61 gccgacggct gtaccgaaga ggtttgggaa aacctgaaaa agttggatgg tctgtctctg
      121 gaatgctttt ccaaggtgat tcagtacggg aaaccgaaga ttgaaccaat cgaagtgcct
      181 tacgaaaaca cgactctccc aggccatttt tacagggtgc cttcggaggc ggaatttaaa
      241 actgccccga ccttggtcat gatcaacggc tatgacggca caaaagaaga aatgtacggc
      301 tgtgcgctgg aagcactcaa gcgaggcatg aactgtttcg cgttcgaggg cccggggcag
      361 ggggagatga tccgcaccag agggattccg tttcgtccag attgggaaaa cgtggtgacg
      421 ccggtgctgg attacctgat tcaaaagttg ggagttgccc cggaccggat cattctgtgg
      481 ggagaaagca tgggcggcta tctcgcgccg cgagccgccg ccttcgacca ccgtgccgcg
      541 gcctgtgttg ccaacagcgg ggtttacgat ttcatgggga cacgcgggcc gaagggaatc
      601 gaccgcaaca cctatttcgg ctatattgaa cagatgccgg aagctgattt tgaagctatg
      661 atgaaaaagc agatggattt ggtggtccag ctgaactggg cgctaaagca cggcatgtat
      721 gtttttcagg cgaaaagtct gaaggattac ttcctgaagg caaaggatta ttacatgggc
      781 gacatttgcg ggcagattgc ctgtccgacg ctcgtcatcg acaccgaggg ggagaataat
      841 tttcccggtc aggcaaaaat gctgtatgat gcgctgaact gtccgaagga ttatctactt
      901 ttcacggaag cagagggcgc cgaggaacat tgtgaggaag gctcgaagct gtatgccaac
      961 tgccggattt ttgactggat cgagaatacg ctgggcggac tcggatggta gaaactgaca
     1021 taatgaccac gctatacaga gaattggaaa gcccgccggg atacgttgac agatcgtatc
     1081 ccggcgggct ttttcactgt tgttattggg gttgtatctg cccggtgtaa atcaaaacgg
     1141 catcccttaa aaattccgca aggcccggct ggtatttgtc gtaataggcc gtaaagcgcg
     1201 ggtcggcaac atacattcgg gcaacgcccg catgtgcctc cggactatac tgagcccaat
     1261 aaaacgacag ccactgccgg tgaaggtcgg ccgcgcactg tgcagcttca ctcgccggat
     1321 tgcctgtttg aaaggcttcc agaaagagtt caatcatctg cttttccagc gccgccgccc
     1381 ggttaaacgt ctcttccgtc atgtcggcca gtttccggtt ggcctgttcc accgtttcca
     1441 cgccgtattt ttcgcggatt tccgctccat acttttgttc attctcttcg gcgatttgcc
     1501 gaagcacctc atccacctgt tttctgctgt aaccgttgat cagaacaccg ttgaaccgaa
     1561 gcacacgaat gtcgtccgaa agaaagttca cttgcctcgc cctcctgccg tttccgataa
     1621 atattattct gtattataat attctgtatt taccgcaagg tgaatccttt ttcgctcggc
     1681 caaccgaaac aaaaaatagt taaaacagct attgaccttg acgcagcgga aaggtgtatg
     1741 attcttttca gtggaaccgg attcacaaaa gaggaaaaga ggtcgatcgc atggaataca
     1801 ccgtaaaaaa gctggcacag ctttccgggg tgagtccccg cgccctgaga ttctatgatg
     1861 aaattgggct gctgagtccg gcaaggatca attcttccgg ctatcggctg tacggcgaac
     1921 gggaagtgaa tctgctgcag cagattctgc tttaccgtga gcttggtctc agccttgagg
     1981 aaatcaaaca gatcgtcacc cagtcggatt ttgatgtgct ggccgcactg caagaacacc
     2041 gcaagcaact tctggaacag cggacaaggc tggacgcgct cattgaaaac gtggaaaaaa
     2101 ccattcgaaa cgcgaaagga gaaacatcga tgaccgattc ggaaaaattc gagggcttca
     2161 agcaaaagct gatcgacgag aatgaacaaa agtatggagc ggaaatccgc gaaaaatacg
     2221 gcgtggaaac ggtggaacag gccaaccgga aactggccga catgacggaa gagacgttta
     2281 accg
//
LOCUS       DATALY010000037         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_353500,
            whole genome shotgun sequence.
ACCESSION   DATALY010000037 DATALY010000000
VERSION     DATALY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353500"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VHR42_01220"
     CDS             <1..1151
                     /locus_tag="VHR42_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3025844.1"
                     /translation="VENRSKKTGEVVRVKKDWNSQETYLSKGYCRVRKAYSSDANGNE
                     AETGSHVSLELECQPMFNRYNSKTVLEGEHSVYAFCDFRITQIKEIKEGKNSITGLVY
                     DHFSGDSMEQAKGWVNSESSFAEMPLRFGYYTPSMGNGKRPLIIWLHGAGEGGNDTAI
                     AYTGNKVVNLSSVNTQKIFGGAYVLAPQAPTMWMDDGSGQYTQDGRSKYGKALKALID
                     EFIHAHTDIDRNRIYLGGCSNGGFMTMRMIIDYPGFFAAAYPVCEALFDSTISDADIT
                     AIQNTPIWFTHAKNDPVVKPELTVLPTYRRLIQAGAADVHFSFFDKIVDISGLFKDSQ
                     GNPFEFFGHASWIYMLNDACRLDYDGSPVKVDGKEVTLLQWLAAKSKV"
     gene            1289..3910
                     /locus_tag="VHR42_01225"
     CDS             1289..3910
                     /locus_tag="VHR42_01225"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEX3025845.1"
                     /translation="MKITKLKANHVSDPLGFELDPLCLSWVVCDSESKRQTAAQVRVA
                     FDPEFHNVCCDSGKSSEISSLCFCPELKLEPYTRYYWQVCVWGDGGDSAVSKTAFFET
                     GKMKTPWSAKWITPNPDEEEHPLLRRSFVVPSPIAKARVYVCGLGLYELEINGQKAGE
                     EYLTPGFHSYDFSLQYQTYDISKLLKQGENAVGAMLGNGWYKGRFGFDGGYTKLYGDK
                     FALICEIRITLENGATLVIGTDENWKWKESPVLKSGIYDGEVYDARKETPGWSSPGTD
                     EESWKPVSELDLGYDKLTARRSLPVVVKERKKPVSLIHTPKGESVLDFGQNMAGWVEF
                     DCSMPSGKETVLQYGEVLQDGCFYNDNLRTAKAEYRYVSNGKERHVRPHFTYYGFRYV
                     KVDGMESVDPSAFTACVVYSDLEKTGTIETSNELVNRLFLNALWGQKSNFLDVPTDCP
                     QRDERMGWTGDAQIFCGTACFNMDTPAFYTKFMADLGNEQKVLGGSVPFVVPCIKPPE
                     PINGIDIGKSQGSAAWGDAATVIPWTTYLFYGDRSLLEKQYPTMKAWVDYIKRQDDKD
                     GSSRLWKTGFHLADWLALDNPDKDSPMGGTDMYFIASAYYYYSASLTAKAAKAIGNEE
                     DAAFYQKLADEVKNAIQREYFTATGRIAIDTQTAMAVALFMNLVPEEFRKRLAADLRK
                     KLEANKMYLNTGFVGTAYLCRALSGNGANDCAYTLFLNEDYPSWLYEVKLGATTVWER
                     WNSILPDGKISGTGMNSLNHYAYGAIVEWMYREMCGLNPTDDAPGFKKVKICPRPDQR
                     IRQVSMSYQSATGLYKIGWNYLDENTVSFDFEIPFDAQAFITLPQGGKVINGPTENEN
                     TEFKLETGKYHFDYQLN"
     gene            complement(4011..>4411)
                     /locus_tag="VHR42_01230"
     CDS             complement(4011..>4411)
                     /locus_tag="VHR42_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3025846.1"
                     /translation="IPTDHPFVHKLTPSMEGAYADMLKILQQKSPVADAYFADNDLIA
                     AGAMRAFQESGYRIPEDVSIIGFDDMPICEFLEPKLTTMTVPKRKLAEMAVERLVNKI
                     KNPKSTVTKIEVSTHLHERLSVKCALKSGD"
BASE COUNT         1209 a          975 c         1165 g         1062 t
ORIGIN      
        1 atgttgaaaa ccgcagcaaa aaaacgggtg aagtcgttcg tgtgaaaaag gactggaatt
       61 cacaagaaac ctacctgtca aaagggtact gccgcgtacg aaaagcatac tcgtccgatg
      121 ccaatggaaa tgaagcggaa accggcagtc atgtctcgct tgaactcgaa tgtcagccca
      181 tgttcaatcg ttataattca aaaaccgttc tggaagggga gcattccgtt tatgctttct
      241 gtgatttccg gattactcag atcaaagaga tcaaggaagg aaaaaactcc attacagggc
      301 ttgtttatga tcatttcagc ggcgacagca tggagcaggc aaagggatgg gtcaacagcg
      361 aatcatcgtt tgccgagatg ccgctgcgtt ttggatatta cacaccaagt atgggaaacg
      421 gaaaacgccc cctgattatt tggctgcatg gcgcgggcga gggcgggaat gacacagcca
      481 ttgcttatac tggtaataaa gtagtaaatc tatcgagtgt aaacacacag aaaatattcg
      541 gcggcgcgta cgttcttgcc ccgcaggccc ctacgatgtg gatggacgat ggatcgggcc
      601 agtatacaca agacggcagg tcaaaatacg gcaaggcgtt gaaggcgctg attgacgaat
      661 tcattcatgc ccataccgac attgaccgaa accgcatcta cctgggtggc tgttccaacg
      721 gcggctttat gacgatgcgg atgataattg attatcccgg ctttttcgcg gcggcatatc
      781 ctgtatgcga ggccctcttt gattcgacaa tttcagacgc ggatatcacc gcgattcaga
      841 ataccccaat ctggtttacc cacgcaaaaa atgatcccgt cgtgaaacct gagttaacgg
      901 tgctgccaac ataccggcgt ttgattcaag ccggagcggc agatgttcat ttctctttct
      961 ttgataaaat cgtggatatc tccggcttat tcaaggattc gcagggcaac cccttcgaat
     1021 tttttggcca tgcttcttgg atttacatgc tgaatgacgc gtgccggctt gattacgacg
     1081 gatctcctgt aaaagttgac ggaaaagaag taacccttct gcaatggctg gcggcaaaat
     1141 ctaaggttta atttactgac agcggcttaa aaaatccatc tgtaaatttt tcaaacaaat
     1201 tagagaaaac ataaaacagg attcgttcat gcacttttcg aaaaggcata agcggatcct
     1261 gattgaattt ggcacaggag gaagcagcat gaaaataaca aagttaaagg cgaatcatgt
     1321 gtctgatcca cttggttttg aacttgatcc gctctgtctt tcatgggtag tgtgcgattc
     1381 tgagtcgaag cgtcagaccg cggcacaggt tcgggtggcg tttgacccgg aatttcacaa
     1441 tgtttgctgc gacagtggaa aaagttcaga aatcagcagt ctctgttttt gtccagaact
     1501 gaaactggaa ccgtataccc gctattactg gcaggtatgt gtttggggtg acggcggaga
     1561 cagtgcggtc agcaaaacag ctttttttga aaccgggaaa atgaaaactc cgtggagtgc
     1621 gaaatggatt acgccaaacc ctgacgagga ggagcatccg ctgcttcgca gaagttttgt
     1681 ggtgccttcg ccgattgcaa aggcgcgggt gtatgtctgt ggccttggtc tgtatgaact
     1741 tgaaatcaat gggcaaaagg ccggagaaga atatctaaca ccgggttttc attcgtacga
     1801 ttttagtctg cagtaccaga cgtatgatat cagcaaactc ttgaaacagg gagaaaacgc
     1861 agttggcgca atgcttggca acggctggta taaaggccgg ttcgggtttg acggcggcta
     1921 cacaaagctt tacggagaca aatttgcgct gatctgcgaa atcaggatca cattggaaaa
     1981 cggcgcgacg ctggtgatcg ggacggatga gaactggaag tggaaagaat cgcccgttct
     2041 gaaaagcggt atttatgacg gcgaagttta cgacgccaga aaggaaacgc cgggctggtc
     2101 gtctcctggt acagacgaag aaagctggaa acccgtttct gaattggatt tgggctatga
     2161 caagttaaca gcgcgccgca gtttaccggt tgtcgtcaaa gagcgcaaaa aaccggtttc
     2221 tctgatccac acgccgaaag gcgagtcggt tctcgatttc ggacagaaca tggcaggctg
     2281 ggtggaattc gactgcagca tgccttccgg taaagagacg gtcctgcaat acggcgaagt
     2341 cctgcaggac ggctgttttt ataatgataa tttacgcacc gcgaaagccg agtatcggta
     2401 tgtttcaaac ggaaaagaaa ggcatgtcag accgcatttt acctattatg gcttccgcta
     2461 tgttaaggtg gacgggatgg aatcagtcga cccatcggcg ttcacggcct gcgtcgttta
     2521 ttccgacctt gaaaagaccg ggacaatcga aacttcaaac gaactggtta accggctttt
     2581 cctgaatgcg ttgtggggac agaaaagcaa tttccttgac gtgccgaccg attgtcccca
     2641 gcgtgacgaa agaatgggat ggacggggga cgcgcagatt ttctgcggaa cagcttgctt
     2701 taatatggat accccggcgt tttacacaaa attcatggca gatctgggaa atgagcagaa
     2761 agttctggga ggttcggttc cgtttgtggt tccctgcatc aagcctccgg aaccgatcaa
     2821 cggcattgac attggaaaat cccaaggctc cgccgcttgg ggcgatgcgg caacggtaat
     2881 tccatggaca acatatctgt tttacgggga cagaagcctt cttgaaaagc agtacccaac
     2941 catgaaagca tgggtcgatt atatcaaaag gcaggacgac aaagacggga gttcgagact
     3001 ttggaaaaca ggtttccatc ttgcggattg gctggcgctc gacaacccgg ataaagacag
     3061 tccgatgggc ggcacagaca tgtactttat tgcctcggcc tattattact attctgcttc
     3121 gcttaccgca aaggctgcga aagcaatcgg caacgaggaa gacgcggcct tctaccaaaa
     3181 gcttgcggat gaagtgaaga atgcaatcca aagggaatac ttcaccgcga cgggcagaat
     3241 tgctattgac acccagaccg ccatggcggt tgcgttgttt atgaatcttg ttccggagga
     3301 gtttaggaaa aggttggcgg ctgaccttag aaagaaactc gaagcaaata aaatgtatct
     3361 caatacgggt ttcgtcggta cggcctatct ttgccgtgcg ctttcgggaa atggtgcgaa
     3421 tgactgcgcc tatacgctct tcctgaatga agattatcca agctggcttt acgaagtcaa
     3481 actcggtgcg acgacggtgt gggagcgctg gaattccatc ctgccggacg gaaaaatcag
     3541 cggaacgggc atgaacagtc tcaaccatta cgcgtacgga gcgattgtgg aatggatgta
     3601 ccgcgaaatg tgcggtctta atccaacaga cgatgctccg ggtttcaaaa aggtgaaaat
     3661 ctgtccgaga ccggatcagc gtatccggca ggtttccatg agctatcagt ctgcaactgg
     3721 gctttataaa atcggatgga attatctcga tgaaaacacg gtgagcttcg attttgagat
     3781 tccgtttgat gcacaggcgt tcattacttt accgcagggc ggcaaggtga taaacggacc
     3841 gacagaaaac gaaaacacag aattcaaatt ggaaacagga aaatatcact tcgattatca
     3901 attgaattaa aaagaggttc cctctcagac gaaacacagt ggattcgttc tggaagggaa
     3961 ccgttttatt agcgcttcac ataatcggaa ataataaaga gcgtggaaat tcaatcaccg
     4021 gacttcagtg cacattttac gctcaaacgt tcgtgcagat gcgtggaaac ttcaatcttc
     4081 gtcacggtac ttttcggatt tttaatctta ttaacaaggc gctccaccgc catctcggca
     4141 agcttgcgtt tcggcactgt cattgtggta agctttggct caagaaattc acagattggc
     4201 atgtcgtcga agccgatgat tgagacgtct tccggaattc ggtatccgct ttcctgaaaa
     4261 gcccgcatgg ccccggcagc aatgaggtcg ttgtcggcaa aataggcgtc cgctaccggg
     4321 gatttctgct gcaaaatttt gagcatatcg gcatacgccc cttccatgga aggcgtaagc
     4381 ttatgaacaa aaggatggtc ggtcgggatt t
//
LOCUS       DATALY010000038         4604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_6658,
            whole genome shotgun sequence.
ACCESSION   DATALY010000038 DATALY010000000
VERSION     DATALY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6658"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1649
                     /locus_tag="VHR42_01235"
     CDS             138..1649
                     /locus_tag="VHR42_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX3025847.1"
                     /translation="MSTKQSKQLNVLKYFDRSSKILLASACLILAAIAAEIFGGVETK
                     FLLLSLSAEAVLFVCLILSKVIQSILSSRDWPVSINRQFSPSEESEEIEVDMDLYNNG
                     QFKSLIKLIEMLQTNIPFKDILKSIYNNFFEYLPYTHIGVSLIDNDSQTIRASYAVSS
                     KIHGSLKNKLIGFQIPVNETSLGKILKSGKPRVINDLEQYLQGREIKDYNRMLLDSGI
                     KSSITFPLINNGIPIGIIFFSSNKTNVYNQEHINFLRTLANSLMLSLEKDILMDDMII
                     SSTLAMATLTEERDLDTGEHLYRMKRYSRFLADLLLSEKVYPEIIDADFISSIERFSP
                     LHDIGKVAIRDSILLKPGKLTQKEFEIMKTHTTYGANVLKMADENLIKLGRSVFKMGI
                     EIAEGHHEKWDGNGYPYGKKGSEIPLSARIVAVADVFDALTSKRSYKPAYSFEESVSI
                     IAGEKGKQFDPVIIDVFLKDIDKFEHYYKSVLSRQLHQQASGAAQEPGPAERV"
     gene            1734..2411
                     /locus_tag="VHR42_01240"
     CDS             1734..2411
                     /locus_tag="VHR42_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025848.1"
                     /translation="MFLYDSVIMLAVILVITFFARVVKIKNKTALVSVILVSIFLGGI
                     ALYVVQCFFGVRFYSQKIDLNQLRQLRVTDIRKGYDQKHFNDFSKISESSGRYENAVC
                     KTYRVKEKGVNSKIDVTVYFFKDKKEADNYFILSQKLYENKTYLPSDPLHSVKKENTY
                     PRYIVSYVKSYYRDYGDIVYLPSKISYLSDVTYQDESMVIVMNEESNKAETGKNAVLK
                     DLISRFR"
     gene            2767..3789
                     /gene="namA"
                     /locus_tag="VHR42_01245"
     CDS             2767..3789
                     /gene="namA"
                     /locus_tag="VHR42_01245"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HEX3025849.1"
                     /translation="MKLFESFKIKDMALKNRIVMPPMCMYQATSDGFPTLFHIGHYAA
                     RALGGAGLIIVESTGVTPEGRITDRDLGIWDDRQISGLKRIVDIIHSNGAKAALQINH
                     AGRKSTSASGHPLGPSAIPFDSKSRAPSEMTRQQILNITAAFKDAAVRANAAGFDALE
                     IHGAHGYLIHEFLSPLTNKREDEYGGSLENRLLFLQNILKEVQSVWPSEKPLWLRVSA
                     SDYQKGGIDGDMMVRMIDQIKPFVDLIHVSSGGLLPVKIDDYPGYQVALAEKIRKQCG
                     IATIAVGLITNEEMAEEILQNGRADLVALGRELLRNPYWPVHTAVAHKIGGYIPESYL
                     RAYPKM"
     gene            3854..4603
                     /gene="gpmA"
                     /locus_tag="VHR42_01250"
     CDS             3854..4603
                     /gene="gpmA"
                     /locus_tag="VHR42_01250"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX3025850.1"
                     /translation="MKLVLVRHGESEWNQKNLFTGWTDVDLSESGVYEATEAGVLLKS
                     ELLDFDVCYTSYLKRAIHTLNLILEQLDREWLPVVKTWKLNERHYGALQGMNKTETAQ
                     KYGDAQVKIWRRSFGVRPPALEENDPRNPMRQEQYRNEKAQELPLCESLQDTIGRVIP
                     YFNRVIKKDIRQGKRVLISAHGNSIRALVKYFENLSEQEIMEVNIPTGIPLVYEFDDK
                     WNILRKYYLGSQELVRAKMQKVASQGVSAGK"
BASE COUNT         1314 a         1051 c         1033 g         1206 t
ORIGIN      
        1 atttttttca cggacattga tctttcctcc taaccttttg ataggattat tattcctgac
       61 ccggctccag ttcatgcatc ccgaatgcta ttttattgct tcttactgac attttacaac
      121 attacgaggt acggcaaatg tcaactaaac agagtaaaca attaaacgtt cttaaatatt
      181 ttgaccggtc cagtaaaata ctgctcgcct cggcctgttt gattcttgca gccatcgcgg
      241 ctgaaatatt cggcggggtc gaaacaaaat tcctgctttt gtctttgtcg gcggaggcgg
      301 tgttgtttgt ctgcctgatt ctatccaagg taattcagag tattttatcg tccagagact
      361 ggccggtatc gataaaccgc caattttccc cctcggaaga gtctgaagag attgaagtgg
      421 atatggatct ctacaacaat ggccagttta agagcctgat taaactgatc gaaatgctcc
      481 aaaccaatat cccatttaaa gatatcctga aatccattta caataacttt ttcgaatacc
      541 ttccctatac ccacatcggc gtttcgctga tcgacaatga cagtcagacg atccgggctt
      601 cctacgccgt ctcatccaag attcacggct ctctgaaaaa caagctgatc ggtttccaga
      661 ttccggttaa cgagaccagc ctgggtaaaa ttctgaagag cggcaagccg agagtcatca
      721 atgatctgga acaatacctt cagggcaggg aaataaaaga ttataaccgg atgcttctcg
      781 attccggtat caaatcctcc ataaccttcc ccctgattaa caacggcata cccatcggca
      841 tcatcttttt ctcaagtaat aaaacaaacg tttataatca ggagcatatc aactttttaa
      901 gaacgctggc caacagtctg atgctcagtc tggaaaaaga tattctgatg gacgatatga
      961 ttatcagctc cacgcttgct atggcgacgc tgaccgaaga gcgcgatctt gatacgggcg
     1021 agcatcttta tcgtatgaaa cgctattccc gtttcctcgc cgatctgctt ttatcggaaa
     1081 aagtataccc tgaaatcatt gatgccgatt ttatcagctc catcgagcgt ttcagcccgc
     1141 ttcatgacat cggaaaagtg gcgatccgcg attcaatcct cctcaagccg ggaaaactga
     1201 cccaaaaaga atttgaaatt atgaagaccc atacgactta tggggcaaat gtcttgaaaa
     1261 tggcggatga aaacctgatc aaattaggca gaagcgtttt taagatggga atcgaaattg
     1321 cggaaggcca tcatgaaaaa tgggacggaa acggctaccc ctatggcaag aaaggctcgg
     1381 aaattcctct gagcgcccgt attgttgccg tagcggacgt atttgacgct ctgacttcca
     1441 aacggtccta taaaccggct tacagttttg aagaatcggt ctccatcatc gccggtgaaa
     1501 aagggaagca gtttgacccg gtgatcatcg acgtattttt aaaagacatt gataaatttg
     1561 aacattatta taaatcggtt ctctcaagac agctgcatca gcaagcgtcc ggggccgccc
     1621 aagagcccgg tccggcagag cgggtttaga ccttttccgt tgacgttatg cggtttaagt
     1681 ggtaaaatgg cttatcatac tgtaaaaaat atcttccccg acaggagaat cctatgtttt
     1741 tatatgatag tgtcatcatg ctcgcggtta ttctggtcat cacctttttc gcccgtgtcg
     1801 tcaaaatcaa gaataaaacg gcgcttgttt cagtcattct ggtcagtatc tttttaggcg
     1861 gaattgccct atatgttgtc caatgttttt tcggtgttcg tttttattcc cagaaaattg
     1921 atctgaatca actcagacag ctgcgggtta ccgatatccg taaaggatac gaccagaaac
     1981 atttcaatga tttttcgaag atatcagaat cctcgggccg gtatgaaaac gccgtctgca
     2041 aaacataccg ggtgaaagag aaaggcgtaa attccaaaat tgacgtgacc gtatattttt
     2101 tcaaagataa aaaagaagcc gataattatt ttatcctcag ccagaaactt tatgagaaca
     2161 agacctatct cccctccgac ccgcttcatt cggtgaagaa ggaaaatact tatccgcgtt
     2221 acatcgtttc ttacgtgaaa tcctattacc gggattacgg tgatatcgtt tatctgccct
     2281 caaaaatatc ctatttatcc gatgtgacgt atcaggacga aagtatggtc attgtgatga
     2341 atgaagagtc caacaaggcg gaaaccggca aaaatgcggt attgaaagac ctgatcagcc
     2401 gctttcggta attctgtatc aggatcttcc tctctcgggt tatttaaaaa cacagctgaa
     2461 aggctgtgtt ttttttgcgc ccgcatatgg aaaatacatg caaataattg tagcaaattc
     2521 aaactgaata agatataata acaggcatga aaagcattat cgtctgaaaa atcaagctta
     2581 agaatatcac tgttttttct ttatctgcgg cttgattatg atgcttattg cacaagtaaa
     2641 atacaatggc ggattatttt aaatgaatat tgtaatttat ttttttataa acttcttata
     2701 cggtcagggg gttgattaat ctatactgtt atagtataat ataatcaaat caaaggagtg
     2761 ttttaaatga aactgtttga aagttttaaa attaaagaca tggctttgaa aaatcggatt
     2821 gtcatgccgc ccatgtgcat gtatcaggcg acatccgacg ggttccccac cctgtttcat
     2881 atcggccatt acgcggccag agcactgggt ggcgcaggcc tgatcattgt ggaatctacc
     2941 ggcgttactc ccgaaggcag gatcaccgac cgcgatctcg gaatctggga tgaccgccag
     3001 atttcaggtc tcaaacggat tgttgatatc attcattcaa atggagccaa agccgcgctt
     3061 cagatcaatc acgccggacg gaaaagcact tccgcctccg gccacccgct ggggcccagc
     3121 gctatcccgt ttgacagcaa atcccgggct ccgtcggaga tgaccaggca acagattttg
     3181 aacattaccg cggccttcaa agacgccgct gtacgggcca acgccgcagg tttcgacgca
     3241 ttggagattc acggcgccca cggctatctg attcacgaat ttttatctcc gctcaccaat
     3301 aaacgagagg atgaatacgg aggaagcctg gaaaaccgtc tcttgtttct tcaaaacatt
     3361 ctgaaagaag ttcaatctgt ctggccgtcg gaaaaacccc tttggcttcg ggtctctgca
     3421 agcgattatc aaaagggcgg catcgacggt gacatgatgg ttcggatgat tgatcaaata
     3481 aaaccttttg ttgatctcat tcatgtcagc tccggcggcc ttctgccggt taaaattgat
     3541 gattaccccg gttatcaggt ggcgcttgcg gaaaaaatcc gtaaacagtg tggaattgca
     3601 acgatcgcag tcggcctgat taccaatgag gaaatggcgg aggaaatcct gcaaaacggc
     3661 agggctgatc ttgtcgcgct cggccgggag ctgcttcgca acccctactg gcccgtacat
     3721 acagcggtcg ctcacaaaat cggcggatat attccggaat cctacctgag ggcctacccg
     3781 aaaatgtaag accctctcaa agccgcctta aaattttacg ccgtcttaac ggatggcccg
     3841 gaaggtgaaa aagatgaaac tggtgttggt aaggcacgga gaaagcgaat ggaaccaaaa
     3901 gaaccttttt accggctgga cggacgttga tctatccgaa agcggagttt acgaagcaac
     3961 agaagcgggc gtgcttttaa aaagtgaact gcttgatttc gacgtttgtt atacctctta
     4021 tctgaaacgg gccatacata cgttaaacct gattctggaa cagcttgacc gggaatggct
     4081 tccggttgtg aaaacatgga aactcaacga aagacactat ggtgcgctgc agggtatgaa
     4141 caaaacggaa actgctcaga aatacggtga cgcccaggtc aaaatctggc ggcgttcttt
     4201 cggcgtccgt cctcccgcgc tggaggagaa cgatccgcga aacccaatgc gtcaggagca
     4261 ataccgaaac gaaaaggcgc aggagcttcc cctctgcgaa agtctccagg ataccatcgg
     4321 cagagtcatt ccttatttta accgggtcat aaaaaaggac atccgtcaag gaaaacgtgt
     4381 tttaatttcc gctcacggca actccatccg ggcccttgta aaatattttg aaaacctgtc
     4441 ggaacaggaa atcatggaag tcaacattcc caccggaatc ccgctggttt atgaatttga
     4501 cgataaatgg aacatcttaa gaaaatacta tcttggcagt caggaactgg tgagggcgaa
     4561 aatgcagaag gtcgccagcc agggtgtgtc ggcaggaaag tgat
//
LOCUS       DATALY010000039         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_353618,
            whole genome shotgun sequence.
ACCESSION   DATALY010000039 DATALY010000000
VERSION     DATALY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353618"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VHR42_01255"
     CDS             complement(<1..968)
                     /locus_tag="VHR42_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX3025851.1"
                     /translation="MLKNMRIGKKLVLTFLFVTLISSIGGFLGLQMMSTMNSRYSEAL
                     VSYGFAQGDIGLFNTEFNNNSALIRDLVIRQNTPELDNTVQQISRSNAKLTQYLDKMK
                     TEMVTDKEKGYYDAIKASLDKYAATRNQAVQFALSQQAAKVSDLVMNECTPRSNEVRT
                     AVETLIKNKSDAGNSIAADLKSQAYRSAFIIVFAVLLSILFSLFIAVRISRGISKPVA
                     ELAEAAKKMAEGDLSVEIKVNTKDEIGQLGAAFSETVSTIHAYIEDIRKNLAKMEQGD
                     LTIKSSLEYKGDFIYLRNSIAGIVNSLNDVISQIQQSAEQVSSGSG"
     gene            complement(1196..1588)
                     /locus_tag="VHR42_01260"
     CDS             complement(1196..1588)
                     /locus_tag="VHR42_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3025852.1"
                     /translation="MNKAAQFLRDCGTFYLATDDNGQPRVRPFGAVIEYEGRTYLCTN
                     NTKNCFKQMQKNPKVEICGTKGGEWIRITGEVVSDPRTEVKERMLEETPSLKNIYRAD
                     DNIFEVLYLKNATATISSFTAKPDTFSL"
     gene            1720..>1921
                     /locus_tag="VHR42_01265"
     CDS             1720..>1921
                     /locus_tag="VHR42_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3025853.1"
                     /translation="MEKEEDFFGKCPYVTSQKLISGKWFILIIHCLTSGVLRFGELQR
                     KIPGITQATLDNYNNGLIGPGHC"
BASE COUNT          497 a          438 c          426 g          560 t
ORIGIN      
        1 tggccggagc cggaggaaac ctgttcggca gactgctgaa tctgggaaat tacgtcgtta
       61 agggaattga cgataccggc aatggaattt cggagataaa tgaaatcgcc cttgtattcc
      121 agactgcttt taatagtaag gtcgccctgc tccattttcg caaggttttt tcggatatcc
      181 tcgatatacg cgtggatggt ggaaaccgtt tccgaaaacg ccgcgccgag ctgcccgatt
      241 tcatcttttg tatttacttt gatttcaacg ctcaaatcac cttcggccat cttctttgcc
      301 gcttctgcca gctcggcgac tggcttgctg atgccgcggg aaatccgaac tgcgataaac
      361 agggaaaaca ggatggaaag caggacggca aaaacaatga taaaagccga ccggtatgcc
      421 tgtgacttta aatcggcggc aatgctgttt ccagcatccg atttattctt gatcagagtc
      481 tccacggccg tgcgtacttc gttggatctt ggcgtacatt cattcataac cagatcagag
      541 accttcgctg cctgctggga aagggcaaac tgaaccgcct gattacgggt agcagcatat
      601 ttatccaaac ttgctttaat cgcgtcatag taaccttttt ctttgtccgt taccatttct
      661 gttttcattt tatcaagata ctgtgtcagc tttgcattgg atcgactaat ctgctgaacc
      721 gtgttatcca gctcaggggt gttctggcgt atgaccagat cacgaatcag cgcactgtta
      781 ttgttgaatt ctgtgttaaa taagccgatg tcgccctgtg cgaatccgta gctgacaagc
      841 gcttctgagt acctgctgtt cattgttgac atcatttgca gaccaagaaa gccgccgata
      901 ctggaaatca gcgtgacgaa cagaaacgta agaactaact ttttaccgat cctcatgttt
      961 ttcagcatac ataatcctct tctctttttt ctttttacaa tcctttttta aaaacagaat
     1021 ttccaaataa atttcctgtt tttacaaccc actttttaaa tatcggttga atgcgtcaaa
     1081 agattaatgg ttttaaggac attttatcga aaaagataaa atagtttgaa acagcagata
     1141 aaaagtcctg tctgccgttt ggcaaacagg acttttattg ggcgcccgcc gaattttaca
     1201 aggagaatgt gtccggtttc gcggtgaaag aggagatcgt cgccgtagcg tttttcaggt
     1261 agagaacctc aaaaatattg tcgtccgccc ggtagatgtt cttcagggaa ggggtttcct
     1321 ccagcatccg ttcctttact tccgttctcg ggtcggaaac aacttcgccg gtaatccgaa
     1381 tccattctcc gcccttcgtg ccgcagattt ccactttcgg gttcttctgc atctgcttaa
     1441 aacagttttt cgtattgttg gtgcagagat aagttctgcc ctcgtattcg atcactgcgc
     1501 cgaacggacg cacgcgcggc tgcccgttgt catccgtagc aagataaaat gttccgcagt
     1561 cccgtaaaaa ttgcgctgct ttgttcataa atataacctc cgggatattt tttatgatat
     1621 aatcatatca cagtttaatt ttatcatgta catcaatttt tacaagtacg attatacaac
     1681 atggtaagta tcataaatga tactaaagga ggagacggga tggaaaaaga ggaagatttc
     1741 tttggcaaat gcccctatgt tacttcgcag aagctgattt ccggaaaatg gttcatttta
     1801 attatccact gcctgaccag cggggtactg cgttttggag agctgcagag gaaaataccg
     1861 ggaatcacgc aggcaacgct ggacaattac aacaacggcc tgatcggccc gggccactgc
     1921 t
//
LOCUS       DATALY010000040         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_613222,
            whole genome shotgun sequence.
ACCESSION   DATALY010000040 DATALY010000000
VERSION     DATALY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613222"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VHR42_01270"
     CDS             <1..1357
                     /locus_tag="VHR42_01270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3025854.1"
                     /translation="GWWTPFAPGIRHGLQSHSKTYAATAVGIAYTEGLLKLDDRIIDI
                     FKDVAPSNPSENLKKLTVHHVLCMGCGMDTMPFPTDHWIRDFLATPVNHVPGTTYMYN
                     STGSTLLGAIVRKLTGLGLQEYLKPRLFDKIGIDADNLRWICMPDGMEIGGGGLFATT
                     EDNLRLMKLYADGGVWNGERILAADYVAKATSLQNQSATESKNNPEAKDNFVGYGYQI
                     WMCRPKGVYRADGAMGQFSIVVPDKDIIIAITENASGAHWAQKTLDVMWEFLDEISDD
                     PELPDDEASSTKLAQRMRRLALPNPAFAPYNKNACSISGKDYIVTNGTFMLENPMMSF
                     MSGSKLSGLSEFRLNFGSDACVFDYVQDSEKKQLTIALDGTRRQNHIASKTSPIATAL
                     VSGAWVGDTVFEMNIRWIETCFVSKFTFFFEGDAVTIKTNNTSIFSTKEPEPIKAKLK
                     H"
     gene            1496..1912
                     /locus_tag="VHR42_01275"
                     /pseudo
     CDS             1496..1912
                     /locus_tag="VHR42_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(1912..2409)
                     /locus_tag="VHR42_01280"
                     /pseudo
     CDS             complement(1912..2409)
                     /locus_tag="VHR42_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          628 a          597 c          599 g          592 t
ORIGIN      
        1 gggctggtgg acgccgtttg ccccgggaat ccgtcacggg ctgcagtctc actccaaaac
       61 atacgcggcg acggcggtgg gaattgccta cacggaaggt ttgctgaaac tggacgaccg
      121 cattattgac attttcaagg acgtagctcc ctccaaccct tcggagaacc tgaaaaagct
      181 gaccgtccat catgtgctct gcatgggctg cggcatggat acgatgcctt tcccaacaga
      241 ccactggatt cgtgattttc ttgctacccc ggtcaatcat gttcccggca caacttacat
      301 gtacaacagc accggctcta ccctgctcgg cgcaatcgtt cggaagctca ccggccttgg
      361 gctgcaggaa tacctgaaac cgagactgtt tgacaagatt ggaattgacg cggataacct
      421 tcgctggatt tgcatgccgg acggaatgga aatcggcggc ggcgggcttt ttgccacaac
      481 ggaggacaat ctgcgcctga tgaagcttta tgccgacggc ggtgtctgga acggcgaaag
      541 aatcttggcg gcagattatg ttgcaaaagc aaccagtctg caaaaccaat cggcaacgga
      601 atcaaagaat aaccccgaag cgaaagacaa tttcgttggg tacggttatc agatttggat
      661 gtgccgcccg aagggcgtat accgcgccga cggtgcgatg ggtcagttct cgattgtcgt
      721 accggataaa gacataatca tcgccatcac ggaaaacgcc agcggcgccc attgggctca
      781 aaaaacgctg gatgtcatgt gggaattcct cgacgagatc tcagacgatc cagaactgcc
      841 cgacgatgag gcgtcgtcga ccaaactcgc tcagcgcatg agacggctgg ctctgccgaa
      901 cccggctttt gccccctaca acaaaaatgc ttgctccatc tcgggaaaag attatattgt
      961 cactaacggt acttttatgc tggaaaaccc gatgatgagt ttcatgagcg gcagcaaact
     1021 ttccggtctt tccgagttcc gactgaattt tggttcggat gcctgcgtct tcgattatgt
     1081 acaggattcc gagaagaaac agctgaccat cgcgctggac ggaacgagac ggcaaaacca
     1141 tattgcttca aaaacttcgc cgattgcaac cgctcttgtt tccggcgcct gggtcggtga
     1201 cactgttttt gaaatgaaca ttcgctggat tgagacctgc tttgtcagca agttcacgtt
     1261 cttctttgag ggagatgcgg taaccatcaa aacaaataac acctccatct tttcgacgaa
     1321 agagccggaa ccgatcaagg caaaactgaa acactgatct gctgtacttc aagcacaata
     1381 cggtcagcat agactcaaca acttatctcc ccccgccgcc ggcaggggga gatatatgca
     1441 atacgaatta attcgaattg ggtgatatct cgtgaaaaaa attgaaaaca aaatcatgct
     1501 gattacttat cctgactcga tcggcaataa tctgaatgat ctgcgtgaaa tcttaaaaga
     1561 atatatcggt gacgccgtcg gcggaattca tcttctcccg tttttcccgt cctccggtga
     1621 ccgggggttc gccccaatga cttatcagga tgtcgaccct gcgttcggcg actggaacga
     1681 cgtgaaaaag ctttccgaag attactatct gatggttgat tatatgatta accatatttc
     1741 ggccaaaagt gaatattaca gggattttct gaaaaataaa gaccagtctc cctatcagga
     1801 tttatttatc cgctttaagg acttttggcc caacggagaa ccgaccaaag aacagggcga
     1861 cgcaatctat aaacggaagc cacgggttcc gtataccgag gccaactttt ccgcggcacc
     1921 gtgagaagcc agatatttca ggttgccctc gatgaaggaa tggcctgttt tggaattgca
     1981 gttgatgtca atctgttctt catcaaaggt gcaccagact ttttcggtgc tgccgtcgga
     2041 aaagttggcc tcggtatagg gaacccgtgg cttgcgctta tagattgcat cgccctgttc
     2101 tttggtcggt tctccgttgg gccaaaagtc cttaaagcgg ataaacaaat cccgataagg
     2161 agattggtct ttatttttca gaaaatcctt gtaatattca cttttggccg aaatatggtt
     2221 aatcatatag tcaaccatca gatagtaatc ttcggaaagt tttttcacgt cgttccagtc
     2281 gccgaacgca gggtcgacat cctgataagt cattggggcg aagccccggt ctcctgagga
     2341 tgggaaaaat gggaggagat gaattccgcc gacggcgtca ccgatatatt cttttaagat
     2401 ttcgcgcaga tcactt
//
LOCUS       DATALY010000041         6028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_699141,
            whole genome shotgun sequence.
ACCESSION   DATALY010000041 DATALY010000000
VERSION     DATALY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699141"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VHR42_01285"
     CDS             complement(<1..752)
                     /locus_tag="VHR42_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEX3025855.1"
                     /translation="MNWFKNFLKSNHRMGLRIVKTGIAVTVCVAVTFFCKLNQPFFAV
                     IATVMSMGKSIDTSFKSGKNKFFGVLLGAVIGYVFFTIAPANAGLCGMGIILTIYLCQ
                     LFRLKGAATLSCFVFSGMMFLYNLGAHRPLQFAISCTADSMIGIGIALVVNLLVMPPN
                     YAEEIKQSFEQLRVQIAKSMDNASAHRQIDTRAVEAAIQKINYTVNLYIAEAKFLRWN
                     DEDVFKISCRLSTYQMILDELRAIQILEPTENG"
     gene            complement(756..1514)
                     /locus_tag="VHR42_01290"
     CDS             complement(756..1514)
                     /locus_tag="VHR42_01290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3025856.1"
                     /translation="MKAERKKLANLSENDYYHIVYLNRQGDILYKACIFDLDGTLANT
                     LNSIAFFANSALEKCGHNTIETSKYRFLVGNGADLLMHRMLNTACGEGNYSEDDVQKL
                     RKTYDALYESDPTHIVTNYDGMQELIRELRQAGIKLAVLSNKPHNCTEAVVEALFAPG
                     TFDACYGQRPEVARKPSPQGALLIAKELGVKPEDCLYIGDTDTDMKTGAAAGMDTVGV
                     LWGFRPRQELEENHAKYIVETPKEIKDILMCGVR"
     gene            complement(1596..2033)
                     /locus_tag="VHR42_01295"
     CDS             complement(1596..2033)
                     /locus_tag="VHR42_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025857.1"
                     /translation="MRDKAVILVCVTGQHDCDRLIRAGRKIADEHHASLQVLSVQPVS
                     AGFETSCEELEYLRQTARDAKAEMTVYFNSDPALVAVSFVKKIRATHLVTGMAEAPVN
                     GFIEIIHKLLPKMPISMVAKDGTVYNICPPNIDTKPGKLVLQH"
     gene            complement(2367..3086)
                     /locus_tag="VHR42_01300"
     CDS             complement(2367..3086)
                     /locus_tag="VHR42_01300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3025858.1"
                     /translation="MNNLHLLNNGSRKNNSKGFYIALGVCLIAIGVAAWTTYDSVVNY
                     TAPDEKTTSSAVAKQTNQAVSGIKVINSEPAAVSSAAPSSQAASSAAPKKTAASVPAK
                     KTVAPALTFSYPVGTSILQKYSGQNLAYSKTMKDWRAHTGTDFAAKQGEAVKAVADGT
                     VKNVYVDGLYGNTVVIAHGDVEAYYCGLYQTTVKKGVKVKQGDKIGTVGTAPMESADN
                     AHLHLSMKKNGKFIDPLTVLK"
     gene            3595..4179
                     /gene="lexA"
                     /locus_tag="VHR42_01305"
     CDS             3595..4179
                     /gene="lexA"
                     /locus_tag="VHR42_01305"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX3025859.1"
                     /translation="MLTESQQKVYDYLKSRTQNGLPPTVREICAATGLKSTSSVHAHL
                     KTLERKGYISRDAGLNRAIHLAGEQNVIQVPIVGRVAAGQPILAVEEVEGYVPYSLPH
                     EGSEEYFALNVRGESMKDAGILNGDIVVIRKTPTAHDGEIVVALIEDEATVKRFYREK
                     DRIRLQPENPDYSPIYTRDVLILGKVVAVFRYYQ"
     gene            complement(4293..4871)
                     /locus_tag="VHR42_01310"
     CDS             complement(4293..4871)
                     /locus_tag="VHR42_01310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX3025860.1"
                     /translation="MKIFDVSRELFGTPAYPGDPQPYRDSIRRMNMGDLCNLSGFYTG
                     CHSATHVDAPRHFIEDGKTIDELGLERFTGRCTVVEASGIITGSDIDAILPKSEKMIL
                     FKGDGKAFLASSAAFALSDAGITLVGTDAQSIEMENEAYQVHKQLLGEEIPILEGLEL
                     SKIQPGSYRLIAFPLLLGGAEASPVRAVLIEE"
     gene            complement(4884..5576)
                     /locus_tag="VHR42_01315"
     CDS             complement(4884..5576)
                     /locus_tag="VHR42_01315"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX3025861.1"
                     /translation="MIGIIGAMRVEVEDLIGIMTDKTAETISSITYHMGTISGVKCVV
                     AQCGVGKVAAAVCAQTMIFKYQPVALINVGVAGGIGENIRIGDIVISRGLVQHDMDTS
                     AIGDEKGLISGLNLITIPASEKLVNLVASLSSEVFGQGVHTGIIATGDQFICDREKFQ
                     AIAADFGASACEMEGASIAQVCYINKVDFVVIRSISDNADEEASVDFNTFAVASAHKL
                     TELIRKLLPDIR"
     gene            complement(5946..6021)
                     /locus_tag="VHR42_01320"
     tRNA            complement(5946..6021)
                     /locus_tag="VHR42_01320"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5986..5988),aa:Asn,seq:gtt)
BASE COUNT         1530 a         1566 c         1366 g         1566 t
ORIGIN      
        1 ccgttttccg tcggttcgag aatctggata gcgcggagct cgtccagaat catctgataa
       61 gtcgacagcc tgcaggatat cttgaataca tcctcgtcgt tccagcgaag gaacttcgct
      121 tcggcgatat acagatttac agtgtagttg attttctgaa ttgcggcttc caccgcgcgc
      181 gtgtcgatct ggcggtgcgc agaggcattg tccatcgact tcgcgatctg gacgcgcagc
      241 tgttcgaaag actgcttgat ctcctcggcg taattggggg gcataacaag cagattgacc
      301 accagcgcga tcccgatgcc aatcatgctg tccgcggtgc agcttatggc aaactgcagc
      361 gggcggtggg caccgaggtt atacaggaac atcatgccgg aaaatacgaa gcaggaaagg
      421 gtcgccgcgc ctttcagcct gaaaagctgg cacaggtaaa ttgtcagaat gattcccatg
      481 ccgcaaagcc ctgcgtttgc gggggcaatg gtgaaaaata catacccgat cacggcgcca
      541 agcagtacgc cgaagaattt atttttcccg gatttgaacg acgtgtcaat ggattttccc
      601 atagacatca ccgtggcaat caccgcgaaa aacggctggt tcagtttgca gaaaaaggta
      661 accgccacgc aaaccgtaac cgcaatgccc gttttcacaa ttcgaaggcc catacgatga
      721 tttgacttaa gaaagttttt gaaccaattc attcattacc tcacgccgca cataagaata
      781 tcctttattt cttttggagt ttcaacaata tattttgcat ggttttcctc cagctcctgc
      841 cgcggccgaa atccccaaag cacgcctaca gtgtccatcc cggcggccgc cccggtcttc
      901 atgtccgtat cggtgtcgcc gatataaagg cagtcttccg gcttcactcc aagctctttc
      961 gctatgagca gcgcaccctg aggcgaaggc ttccgcgcca cttccggccg ctggccgtag
     1021 caggcgtcaa acgtgcccgg cgcgaacagc gcttcaacca ctgcttccgt gcagttgtgc
     1081 ggcttattgg aaagaaccgc cagcttgatt cccgcctgcc ggagttcccg aatcagttcc
     1141 tgcatcccgt cgtaatttgt cacgatatga gtgggatcgc tttcatacag cgcatcgtag
     1201 gtcttcctca gcttctgaac gtcatcctcc gaatagtttc cctctccgca cgccgtattc
     1261 agcatccggt gcatcagcaa atccgcgccg ttgccgacca aaaagcggta ctttgaagtc
     1321 tcaatggtat tatgtccaca tttttcaagc gcactgtttg cgaaaaatgc aatggaattc
     1381 aatgtatttg ccaaggttcc gtctaaatca aaaatgcaag ctttatacaa aatatcaccc
     1441 tgtctgttaa gatacacaat atgataataa tcattttcac tcaaatttgc aagctttttc
     1501 cgctctgcct tcatggaatc ggcaatcctc acggtaataa gaaaaagccg gagtatccgc
     1561 tccggctatg tattcctgca taatattcga atttattaat gctgcaaaac cagctttccc
     1621 ggtttggtat caatatttgg tgggcatatg ttataaacgg ttccgtcctt cgccaccatg
     1681 gaaatgggca tcttaggcaa cagcttgtga atgatctcga taaatccgtt taccggcgct
     1741 tccgccattc ctgttacaag atgggtcgcc cggattttct taacaaagct cacggcaacc
     1801 aacgccggat cactgttaaa ataaacggtc atttccgctt tcgcatctct tgccgtctgt
     1861 cgcaaatatt caagctcctc gcagcttgtt tcaaatcccg cggaaaccgg ctgaacactc
     1921 agtacctgca aagaagcatg atgttcatcg gcaatttttc ttcccgcccg aatcagtctg
     1981 tcgcaatcgt gctgcccggt aacgcaaaca aggattactg ccttgtctct cacttctcta
     2041 cctcctttta cttcataatt tgattataac aaatctctgt taagtttatg gtaatagaag
     2101 ttgtataaat cattaacaaa gtgttaatta ttcattcgac aaattttgtg atatttatat
     2161 aatttattat attatatcat ggaatccatt ggaattcaac cgctaaaagt cttatgcttc
     2221 tccgcttttc atttataacg ccgctgcgtt ccggtaagtc gttcgtatat gtcgcaggac
     2281 aagccggggg tacaaaaaaa tagccgcaga acgccattgt cctgcggctg tgatttgccc
     2341 ctaagcgctt agagtacggg ggcaggttat ttcagaacgg tcagagggtc aataaatttg
     2401 ccgttcttct tcatagacag gtgcagatgc gcattgtccg cgctttccat cggagcggtg
     2461 cccactgtgc cgatcttatc cccctgcttg accttgacgc cctttttaac ggttgtctgg
     2521 tacagcccgc agtaataggc ttccacatcg ccgtgggcta ttacaacagt atttccgtac
     2581 aatccatcca catagacgtt cttaaccgta ccgtcggcca cggctttcac cgcttcaccc
     2641 tgctttgctg caaagtcggt gccggtatgg gcgcgccagt ctttcatcgt cttggaatag
     2701 gctaaatttt ggccgctgta tttctgcaga atactggtgc caacaggata ggagaatgtg
     2761 agcgccggag ccacggtctt ttttgccgga accgacgccg cggtcttctt gggtgccgca
     2821 gaggatgctg cctgggacga cggtgccgca gaggataccg cagccggttc gctgttaatt
     2881 actttgatac cgcttaccgc ttgattcgtc tgttttgcca cggcgctgct tgttgttttt
     2941 tcatccggtg cggtatagtt caccacgctg tcataggtag tccatgcggc tacgccgatt
     3001 gcgatcaggc acacgcccag tgcgatataa aatcccttag aattattttt tctcgatccg
     3061 ttattcagta gatgtaaatt attcattcgt gccacctccg aatatatttt gaccaaatat
     3121 ttctggttat attcggcgga atcaattaat ttttaaaaga atgtacgcaa attcccccgt
     3181 tattttacaa aaaccgacag ggtatacggc agttccccgc agagccgggc attggctgca
     3241 aaaaaaccgg caggccgcga aaaaacacgt tatattttta tgtcatacca aacgtttata
     3301 cggaaggaga ataaaaattt tggaataaca aaaagaaaaa gaacccaatc caaaatgatc
     3361 gggttctttt tcattagctt gaggggtata ttgaggaggg tagaacatga atcaaggatg
     3421 gtgcggctcc atcttcttga ttacaaccac attatagccg ttttgtcaaa atcagttgtt
     3481 acgcagctat gaacttctta tgaatttttg agaaatccaa acatttgttt gtattttccc
     3541 gaaagatatg ttataatact ctcatgaacc aaaggaacgg ggaggaccat cattatgttg
     3601 accgaaagcc agcaaaaggt atatgattat ctgaaatcca gaacccagaa cgggcttccg
     3661 ccgactgtgc gggaaatctg cgccgcaacc ggcttaaaat ccacctccag cgtacacgca
     3721 cacctgaaaa ctctggagcg aaagggctat atcagccgtg acgccgggct aaaccgtgca
     3781 atccatcttg ccggggagca aaacgtgatt caggtcccca tcgtcggcag agtcgcggcc
     3841 ggccagccga ttctggccgt tgaggaagtg gaaggctatg tgccatacag tcttccgcac
     3901 gagggcagcg aagaatactt tgccctgaac gtccgcggcg aaagcatgaa ggacgccggc
     3961 atcctgaacg gcgacattgt agtcatccgc aaaacgccca ccgcgcacga tggcgaaatc
     4021 gtggtggcgc tcattgagga cgaagcaacg gtaaagcgct tttaccggga aaaggaccgg
     4081 atacgcctgc agcccgaaaa cccggactat tccccgattt acacgcgcga cgtactgatt
     4141 ttaggcaagg tcgtcgccgt attccggtat tatcagtaat acaaagtacg taaagactgt
     4201 cgcgatgtta aagcagttca aacaataatc aagcctgaaa gagcgttcct gttctttcag
     4261 gctttttcat atccattata aaatattctt ttttactcct caatcaaaac agcgcgtact
     4321 ggtgaagctt cagccccgcc cagaagcagg ggaaacgcaa tcagccggta actgcccggc
     4381 tggatctttg acagttccag accctccagg atcggaattt cctctcccag cagctgttta
     4441 tgaacctgat acgcttcatt ttccatctcg atactctggg catccgttcc caccagcgta
     4501 attccggcgt cggaaagcgc aaaggcggcg cttgaggcta aaaaagcctt tccgtcccct
     4561 ttgaaaagga tcattttctc cgatttcggc agaatcgcgt cgatatccga gccggtaata
     4621 ataccgcttg cctccacgac ggtgcatctg cccgtaaacc gctccaaacc aagttcgtcg
     4681 atcgtttttc catcctcaat aaagtggcgc ggcgcgtcca cgtgggtggc gctgtgacaa
     4741 cccgtgtaga atccggaaag gttgcacaga tctcccatgt tcatccggcg gattgaatca
     4801 cggtacggct gggggtctcc aggatatgcc ggcgtgccga acagttcccg tgagacatcg
     4861 aagattttca cggtatcacc ttcttatcgg atatcgggca gtaatttgcg gatcagttcc
     4921 gttaatttat gggcggaagc aaccgcaaac gtattgaagt caacactggc ctcctcatcc
     4981 gcgttatcag agatggaacg gatgacaaca aagtcgacct tgttgatata gcagacctgc
     5041 gcgatgcttg cgccttccat ttcgcaggcg gacgcgccga aatcggcggc aatcgcctga
     5101 aacttttccc ggtcacagat aaactgatcc ccagttgcaa taattccggt atgcacgccc
     5161 tgcccgaata cttcactgga tagggaggcg accagattaa ccagcttttc gctggccgga
     5221 atggtgataa ggttcagacc gctgataagg cccttttcat ccccgatcgc ggaggtatcc
     5281 atatcatgct gaaccaaccc acgcgaaatg acgatatcgc caatacggat gttttcgccg
     5341 atcccgccgg caacgccgac attaatgaga gcaaccggct gatacttaaa tatcatcgtc
     5401 tgcgcgcaga cggccgcggc caccttccct acgccgcact gagccaccac gcattttaca
     5461 ccgctgatcg ttcccatatg gtaagtaata ctgctgatgg tttcagctgt tttgtcggtc
     5521 atgataccga tcaaatcttc cacttcaaca cgcatcgcgc cgataattcc gatcatatta
     5581 gcaccccgca attttttatc gtagataaat tataatgcaa aatatcagga aaagcaaatt
     5641 tcaccaacaa atgaaaaagg gcggcctaag tcgtcccttt tatcatagct gtactacctc
     5701 ttctggatca agattccgcc ttttagtaga tacactgcga aatgatcaaa ctcctccaag
     5761 gttatgctgt ctctccaggg ctttaagatt ggtatgcaaa aatatgttct gctttaatac
     5821 agaaaggggt atttgatgct actcctgatt ttttcaattt tgggttttac tccaaccatt
     5881 gacaaagagc caaaagtttc cgtataacag caaaaaacca cttgttaaaa aacaagtggt
     5941 ttttttggct ccccaagttg gactcgaacc aacgaccctg cggttaacag ccgcatgctc
     6001 taccggctga gctattgagg aatatttg
//
LOCUS       DATALY010000042         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_268258,
            whole genome shotgun sequence.
ACCESSION   DATALY010000042 DATALY010000000
VERSION     DATALY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268258"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VHR42_01325"
     CDS             <1..361
                     /locus_tag="VHR42_01325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3025862.1"
                     /translation="YSGSGYISCPFGGYSGHTGADYACAEGTPVKAADAGTVVSAGWD
                     GGYGNCVKISHGNGIVTIYAHCSALLVSTGEKVYKGQLISRVGHTGHVIGNPGDHLHF
                     EIRVNSVAVNPFRFVTP"
     gene            770..>1726
                     /gene="murA"
                     /locus_tag="VHR42_01330"
     CDS             770..>1726
                     /gene="murA"
                     /locus_tag="VHR42_01330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX3025863.1"
                     /translation="MEKFLITGGHQLTGEVDISGAKNAAVAIIPATILAEGPCRIENI
                     PNISDVSIIIEILHKLGAQVRLINKTTLEIDTSRLNEPIVTYELARKMRASCYFLGAL
                     IGRFSHAQVAMPGGCDFGVRPIDQHIKGFEALGASISLEHGMVEANTLKLTGNQIYLD
                     VVSVGATVNIMLAAVKAEGLTVIENAAKEPHIVDLANFLNSIGADVRGAGTDVIKIRG
                     AQKLHGTTYSIIPDQIEAGTFMAATAATGGSVLIKNVIPKHLESISAKLVEMGVTIEE
                     FDDSILVRRVGPLYKANVKTMPHPGFPTDMQPQMAVLMSLARG"
BASE COUNT          465 a          362 c          429 g          470 t
ORIGIN      
        1 ttattccggc tccggatata tatcctgccc gttcggcggc tattccggcc ataccggagc
       61 agattacgcg tgcgcggaag gcacgcctgt caaagccgcg gatgccggaa cggttgtctc
      121 tgccggttgg gacggaggat acggaaattg tgttaaaata agccatggca acggaatcgt
      181 gaccatttac gctcattgca gcgcgcttct tgtttcaacg ggggaaaagg tgtacaaagg
      241 gcagctgatt tcaagggtcg ggcatacggg gcatgtcatc ggcaaccccg gcgaccacct
      301 gcattttgaa atccgcgtaa attctgttgc ggtcaatcct ttcagatttg taacaccctg
      361 attacaattt ggaaaaatcg ccacagcttt gccaaagctg tggttttttt ctaacacctg
      421 tgatataata ttaaagttat ttatagtgaa atattccaaa atttatgcgg atcttaaatg
      481 attgcatgta ttgtgttttt tacatagaaa aagcagcaga aaatcatgga taaaaatgaa
      541 attaatggaa aacatattgt tatccgattt ttatattcct gcgaaatcag gcggttttac
      601 gctcatagcg cggattataa aagatacaac tgcgataact cgttttttct tcttgtgata
      661 ccattttatt tctgatatta ctgaaatttt gcatattaat ttttctatgc ttgtgaaaga
      721 ctattagtat tggatcaaac agatttaaat tcaataggga gcggttgcgt tggaaaaatt
      781 tttaattacc gggggccatc aattgaccgg tgaagtcgat atcagcggag cgaaaaatgc
      841 cgctgttgcg attattccgg caacaattct tgctgaaggg ccctgcagaa tagagaatat
      901 tccgaatatc agtgatgttt cgattattat agagattctt cacaaacttg gagcgcaggt
      961 ccggctcatc aataaaacga cgcttgagat tgacacgtca cggctcaatg agccgattgt
     1021 cacttatgag cttgccagaa aaatgcgcgc gtcctgttac ttcctcggag cattaatcgg
     1081 gcgtttttcg cacgcgcaag ttgccatgcc gggcggatgt gatttcggtg tcaggccgat
     1141 cgaccagcat attaaaggat ttgaagccct tggggcaagc atatctctgg aacatggtat
     1201 ggtggaagcg aacacgctga agttaacggg caaccagatt tatcttgatg tggtttcggt
     1261 tggagcaacc gttaatatca tgcttgcagc ggtgaaagcg gaaggcctga cggtaattga
     1321 aaatgcggca aaggaacctc atattgtaga tttagccaac tttctaaatt ctatcggggc
     1381 ggatgtccgc ggagcgggca ccgacgttat aaagatccgg ggcgctcaga agctccacgg
     1441 aacgacttac tccattattc ccgatcagat tgaagcaggc acgtttatgg ctgccacagc
     1501 ggcgaccggc gggagtgttc tgatcaaaaa cgtgattccg aagcatctgg aatcaatttc
     1561 agcaaaactt gtggaaatgg gcgttaccat cgaagaattt gacgattcta ttctggtcag
     1621 gcgtgtcggc ccgttatata aggcgaacgt caaaacaatg cctcatcccg gtttcccgac
     1681 ggacatgcag ccgcaaatgg ccgtgttgat gtcacttgcc cgggga
//
LOCUS       DATALY010000043         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_441397,
            whole genome shotgun sequence.
ACCESSION   DATALY010000043 DATALY010000000
VERSION     DATALY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441397"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1282)
                     /locus_tag="VHR42_01335"
                     /pseudo
     CDS             complement(1..1282)
                     /locus_tag="VHR42_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926202.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
     gene            complement(1923..2364)
                     /locus_tag="VHR42_01340"
                     /pseudo
     CDS             complement(1923..2364)
                     /locus_tag="VHR42_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
     gene            complement(2478..3298)
                     /locus_tag="VHR42_01345"
                     /pseudo
     CDS             complement(2478..3298)
                     /locus_tag="VHR42_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271426.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
BASE COUNT          784 a          851 c          759 g          904 t
ORIGIN      
        1 gtcatctgta ccgcgcccca gcggtcaaag gcaatctcac ggatattgta atgcgttccc
       61 agttcttcta tgaagctttc aataaagccg taatggacaa cgttaccttc ggtggtctgt
      121 aaaaaaacct gccgttccca cagatcataa ttcatatggt cgcgccggac gcgcagatca
      181 aggttgtccc cgggaatcca gaagtatgga agaatgctgt atttatcgtt ctcatcttct
      241 ggcggaaaaa ccagaacgaa tgcggtgata tcggtcgtac tggaaaggtc gagtccgccg
      301 tagcagaccc ggccttccag tttctttgga tcagccgcaa aagcgcactt gtcccatttc
      361 tccataggca tccaccggat ggactgtttc acccactggt tcagacgaag ctggcggaaa
      421 gcgttttctt ccgccggatt ctcttttgcg ctctcgcatg ccgtccgtac cttgtcaatt
      481 ccaaccgtaa tccccaacga cggattcgct ttcttccaca ccttcgggtc ggtccaatcg
      541 tcggattcgt ccgcgccgta aatcaccgga taaaaggtcg ggtcatgctt gcggcctgcc
      601 agaatgtcct tggctttctg atgcacttcc caacagatgg agttggtgtt gtctcctgcc
      661 gtcgtaatca gaaaatacag cggctgcatg cgggcatcgc cggagccgcg cgcataacgt
      721 catagagttt acggtttggc tacgcgtgca gttcatcaaa tactactccg tgaatgttca
      781 ggccgtgctt ggtgtaggct tccgccgaaa gcacctgata gaaactgttg gtcggaagat
      841 acaccagtcg cttgagggaa gccagtattt ttacgcgctt ggcaagcgcc ggacactgct
      901 tcaccatatc ggaagcaacg tcaaagacga tcgacgcctg ctgccgatca gcagcacaac
      961 catacacttc ggcgcgttcc tcatgatcgc cacaggtcag aagcagagcc acagcggcgg
     1021 caagctcgga ttttcccatc ttcttcggga tctccacata ggcattggtg aattgccgat
     1081 agccgtcggg tttcaaaata ccgaaaacat cccggatgat ctgctcctgc cagtcgatca
     1141 gttcaaaatt ctttcccgcc caggttcctt tggtgtgagc aagacattcg atgaagccta
     1201 cggcgtagtc ggcggcggcc ttgctgtaaa ccgaatcctt cgccttaaaa ttggtgggga
     1261 tgtacttttt cagtttgcgt ataattgccg cctcctaaat gtgcagaaaa aaaggaactc
     1321 catttctgaa gttccttgaa actatatatc tgcaagctat ttcttggaag ttttatgctt
     1381 taaagcaatt actctcgaca aattgaagaa tactatttct gaaatcataa tgccgattaa
     1441 tccaaaggcc caaaacggaa ccccccaata ctcaaatcga aacagcataa attggataaa
     1501 agggcctaat gcaaatatta ttaatcctgc aattgcacaa aggatcttga taactatgaa
     1561 tgatttcata ttaattcctc ccaataggta atctctgctc tcatgcgagc atgttatata
     1621 aatcgggaaa tatcagtcct attataaaaa gtatccccaa aaacagagca aatacagtta
     1681 tcaccagtaa aatacttacg attattgtaa atagcgtttt gcttttattt aatttataat
     1741 ccggattctt tctttccata aatctgttcc atagtgaaag aacagcatct atcattaatt
     1801 caagaacacc ttcaaatagc gcatccaaac tatttacctc acattacttt ttatgtagtt
     1861 tgattatatc atgcacagat taaaaggtaa tgaggtatat tatggcaata aagagagttt
     1921 tcttatagtt ttctagttac tgcctgtttc agatcattca aatcaaatcc ttccgagagg
     1981 taaccttgta aaatgacatt gtaataatag cagctcggtt ttccgagcgg tctgccctcg
     2041 ttcatgatgt agaccatcgc ctcgaccgat ttgccgttca gagtgaccga catggtttct
     2101 ttccggtaga ggtacggcca cccttcatag tggtccagcg ctacttcgtc ggcgggagtg
     2161 atcttccaca gcagaaccag tacacttcca cctttttgcg gttcaatggt agcgcgggtt
     2221 acaattcccg ccgcgaaaca gaagcctgta gtattccaag gttgccgctc cggcaatctt
     2281 ggcggttgga caccgcgcaa ccatttgctc cagattcaaa ttcgagccat aggctaagta
     2341 aagcattgtt tgttctcctt gcattttgtt cgggccgtgc agccccgtgt tgctttttct
     2401 tctccaggcg gaaccaccgt tgccagtaag gactggcggg gcacacggcc ccaccgttgc
     2461 cttcaccggc ggttccttca ggcagcggag ccgaaccgcc atgccgccga accgtccagt
     2521 tggctgcaaa ggtgttcccg gcagtttttg aattcttctc caatcaggcc gatgcggttc
     2581 aggtacgtac gcatcgcgaa cttttcattt tcaacctgtg gctttttagc tgatgcgcat
     2641 ttctgcgtca gcgcctgatg gttcagcgcc agagctaaaa caatgtagct tcggattttc
     2701 ccggcgtgaa gctcgctgtt gaagcctctc agttccaccg tatggttgcc gttgaaaaag
     2761 ctgtgcaagt tcaggaaatg gtaccggctg ctgtggtaat gggtgctgcg gctggaattg
     2821 taacccgcgt accaaatctc ttccattttc cgcatagtct tcggcttttt ccggttcatg
     2881 gtttccacca gcgtatcgtc catctttttg caaaaccgca tcctcgccgg gtcaatctgc
     2941 tcagggcttt gtagaaaagg tcgttcttgc tggcgatgat gttgacaaaa ttccggatgc
     3001 tacgcggagt gtggttcgcg ctgtcgaggt ggatatgaat tccgcaggag ttgttcgcaa
     3061 acccgcctgc atggcgaatc cggcggatca gctcctgcag ggtatcgatg tcctcgcggt
     3121 aggtgagaat cgggcttacc agttcaacgc tgtattcgcc cgttgcggtg ataacccggc
     3181 cccgttcctt tttctcggtg cgaatactgc catcgctcat ggttttccaa actctgccgt
     3241 ccggcgcggt aatctttttc gtatcgtagt agtcccgacc gtcaataatc attccgcc
//
LOCUS       DATALY010000044         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_182493,
            whole genome shotgun sequence.
ACCESSION   DATALY010000044 DATALY010000000
VERSION     DATALY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182493"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1846)
                     /locus_tag="VHR42_01350"
     CDS             complement(<1..1846)
                     /locus_tag="VHR42_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX3025864.1"
                     /translation="MIFILLAAAVISAVLQEITDSIIILLVVLLNGIIGYIQEARAEK
                     AIDALKKMSTPKAVVRREGKTEEIDASQLVPGDIVLLEAGRVVPADLRLIESSNLKIE
                     ESALTGESVPSEKDSGFVAEGDTAVGDRINMAYMTTPVTYGRGTGVVTATGMDTEIGR
                     IAKALEEDDDEMTPLQKRLADLGKLLGILTVALCVVLFLVAVLQKRDVLDMMLTAISL
                     AVAAIPEGLPAVVTIVLALGVQRMVKSRTIVRRLPSVETLGAVNVVCSDKTGTLTQNR
                     MTAVKTYFNGKLTEPKELNASDAAFYLEGFTLCNDASIEDGKNVGDPTETALLDMSKL
                     FELSRKGLESKYPRINEIPFDSDRKMMTTVHDYGDKKLSYTKGALDVILKHTKRIFVS
                     GKMREITPEDIENINGAASQMAHDALRVLALSCRESDGEAVEEDLCFIGLVGMIDPAR
                     PEAKEAVESCRRAGVTTVMITGDHKETALAVARDIGIASDPSQCISGEELNRMTQEEL
                     NQKVMNLRVFARVSPEHKVMIVRALKASGNIVSMTGDGVNDAPSLRAADIGLAMGITG
                     TDVAKGAADMILTDDNFATIKKAIQEGRNIYNNIKKSAIYLLSSNIGEI"
BASE COUNT          411 a          516 c          478 g          543 t
ORIGIN      
        1 cgatctcgcc gatgtttgat gaaagcaggt aaattgctga tttcttgata ttgttatata
       61 tattgcggcc ttcctggata gcttttttaa ttgtcgcaaa gttgtcgtcc gtcaggatca
      121 tatccgcggc gccttttgcg acgtctgtgc cggtgatccc catggcaaga ccgatgtcgg
      181 cggctcttaa agacggtgcg tcgtttacgc cgtcgcctgt catcgaaacg atattgccgc
      241 tcgccttgag cgcccggact atcattacct tgtgctccgg agaaacgcgg gcgaaaacgc
      301 gcagattcat aaccttttgg tttaattcct cctgcgtcat cctgttaagt tcctcgccgg
      361 agatacactg tgacgggtcg gacgctatgc cgatatcgcg tgcgacggca agcgccgttt
      421 ccttgtggtc gcctgttatc atgacggttg ttacaccggc tctgcggcag ctttcaacag
      481 cctcttttgc ttccgggcgc gccgggtcga tcatgccgac aagcccgatg aagcataagt
      541 cttcctcgac cgcttcaccg tcgctttcgc gacagctcag ggcaagcacc cgaagggcgt
      601 catgggccat ttgggacgca gcgccgttga tgttttctat atcttcaggt gttatttccc
      661 gcatttttcc gctcacaaaa atgcgttttg tatgctttag gattacatca agcgcgccct
      721 ttgtataaga gagtttttta tctccgtaat catggactgt ggtcatcatt ttgcggtcgc
      781 tgtcaaacgg tatctcgttt attctcgggt atttgctttc aagacctttg cggctgagtt
      841 cgaataattt gctcatgtca agaagcgcgg tttccgtcgg atcgccgaca tttttcccgt
      901 cttcaatcga agcgtcgttg caaagagtaa agccttcgag ataaaaggcc gcgtcactcg
      961 cgttaagctc ctttggctcg gtgagttttc cgttaaaata tgtttttacc gctgtcatgc
     1021 ggttttgtgt cagcgttccc gttttatccg agcagacaac gttgactgcg ccaagtgttt
     1081 cgacagacgg aagtcttctg acaatcgtgc gtgatttgac cattcgctga acaccgaggg
     1141 caagcactat cgttactaca gcaggaagtc cttcggggat agccgcgacc gcaagtgaaa
     1201 ttgccgtgag catcatgtcc agaacatcgc gtttttgaag gacggcaaca aggaaaagaa
     1261 caacgcacaa agccacggtc agaattccga ggagtttacc gagatcggca agccgctttt
     1321 gaagaggtgt catctcatca tcgtcttctt cgagcgcttt tgcgattctg ccgatttccg
     1381 tatccatacc tgttgccgtt acgacgcctg ttccgcgccc gtaagtaacc ggagtagtca
     1441 tatatgccat attgattctg tcaccgactg cggtatcgcc ctccgcgaca aatccgctgt
     1501 ccttttccga cggaactgat tcacccgtca gcgcggattc ttcaattttg aggtttgagg
     1561 actcgataag gcgcagatcc gcggggacaa ctctcccggc ctccagcagg actatatcgc
     1621 ccggaacaag ctgcgacgcg tcgatttctt ccgttttgcc ctcccgtcgg actacagcct
     1681 tcggggtcga catttttttc agcgcgtcga ttgccttttc cgctcttgct tcctgaatat
     1741 aaccgataat tccgtttaac agcacaacaa gcaggattat aatgctgtcc gtaatttcct
     1801 ggagaacagc tgaaatcacc gccgccgcga gcaggataaa aatcatcgga tcgttgaatt
     1861 gcaacaaaaa ttttataacg cggctatatt tccgcgcttg ccgcgggtat cgcagggtct
     1921 ctgacaaata caattcttgt gctcggct
//
LOCUS       DATALY010000045         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_8569,
            whole genome shotgun sequence.
ACCESSION   DATALY010000045 DATALY010000000
VERSION     DATALY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8569"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VHR42_01355"
     CDS             complement(<1..605)
                     /locus_tag="VHR42_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX3025865.1"
                     /translation="MNIKITSENLNLHYGDVHALKNVNLEAYENRVTALIGPSGCGKS
                     TFLKTLNRMNDLIAGVRIEGKVTLDGEDIYARDTDVTLLRKRVGMVFQKPNPFPMSVY
                     DNVAYGPRIHGIKSRSQLDEIVQRSLEDAALWDEVKDRLKKNALGFSGGQQQRLCIAR
                     ALAVEPEVVLMDEPTSALDPISTMKIEELISVLKDKYSIVIV"
     gene            complement(606..1493)
                     /gene="pstA"
                     /locus_tag="VHR42_01360"
     CDS             complement(606..1493)
                     /gene="pstA"
                     /locus_tag="VHR42_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX3025866.1"
                     /translation="MKFSHKRALFSDRAATGVLYAVACFFLLLLAAFTLYILYKGALA
                     CKPEYLAFNRGGIGVEFFNTIYLVLISLVISLPIGIAAGIYMAEYAGNGRLTRFLRTC
                     IETLSSLPSIVVGLFGYLVFLVLTGSKWNLLAGAAAVSILNLPLLTTVTEDALEGIPA
                     IYREGSLGIGATHWQTIVNVLFPAVVPRIVTGVIMAAGRGFGEAAALLFTSGMSSDVN
                     FNNWNPMSVTSPLNPFRPADTLAVHIWALKTEGIDANANQLADLSAAVLILMVFVFSL
                     GSRIIGNHLERKISGKGGK"
     gene            complement(1490..2386)
                     /gene="pstC"
                     /locus_tag="VHR42_01365"
     CDS             complement(1490..2386)
                     /gene="pstC"
                     /locus_tag="VHR42_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008858636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX3025867.1"
                     /translation="MDLRRKIRKEYLGRAVVTLFGFFIILLTLSIGAFLFYKGMATFT
                     VHKHSILEFLFSSKWSPAQIGQGGSVGAAIFIFGSITISLLALLIATPFSLAAALFLT
                     EISPHLGRKLLQPAVEIFVGIPSVVYGWVGLTVLVPAIAKLFHLPYGYSVLAGAIVLA
                     VMIFPTITSVSSDAIRNVAEEYKEASYGLGSTRWQLIRHVIVPAALPGILTGVVLGLA
                     RAFGEALAVAMVIGKMQAFPGNILKPTVNLTSEITADMGNTISGSEWNDALWTMALFL
                     LVISFIFIVLIRIVGRMGENKK"
     gene            complement(2584..3480)
                     /locus_tag="VHR42_01370"
     CDS             complement(2584..3480)
                     /locus_tag="VHR42_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004009306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3025868.1"
                     /translation="MKKTLALFMATALIGLSMTACGNSDNASSASQSGSSSQSLSGSI
                     TAAGSSALQPLVQAAADDLKAKNPDLSITVNAGGSGQGLQNALNKTVDIGNSDVFAEE
                     KLDASSSKTLVDHQVCVVGVAAVVNPDVKVTSITKDQLIKIFTGKIKNWKEVGGNDEA
                     IVIINRPSSSGTRALFKKYGLDGNDEVTGQALTEDNSGTLRQNVTQTKGSISYLAFSY
                     LDSSVNALSIDGVKPAYDNVYSGKYPIWGYEHMYTNGEASGAAKALIDYMKSDSFSST
                     VTAKGYGLIGKMTVKRAAPAAK"
     gene            3759..4313
                     /gene="yfcE"
                     /locus_tag="VHR42_01375"
     CDS             3759..4313
                     /gene="yfcE"
                     /locus_tag="VHR42_01375"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX3025869.1"
                     /translation="MKLFFIADIHGSAYYLKKTLAVYEQEKADYLVILGDELYHGARN
                     PLPEEYNPKEVAGLLNDYSGKIIAVRGNCDSEVDQMVLNYPIMSDYSTILYHGKRLFL
                     THGHLYQENNMPGLCDGDVFFYGHTHVLRAEKKDGVTIINPGSITLPKENLPHTFGLL
                     ENDVFEIRDLNGGVVKKIRFGKVL"
     gene            4330..5406
                     /locus_tag="VHR42_01380"
     CDS             4330..5406
                     /locus_tag="VHR42_01380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3025870.1"
                     /translation="MSENKMIKWKDKIRSGAVFFCLWGLSILYLGFNCFYHLDRTMVM
                     SYDEARHGVNAFEMMRTGNYIVSTFNYHNDYWNLKPPMSFWAIILDFKIFGTTVLGLR
                     FYSALALFLTILMISVFLLKRYGKIECLVITTMLACCFSFYAGHFGRNGDADALYGLF
                     LTAGALALFYVKDNHAFLYLTGAMFSLAFLTKSWHAFILLAVTAACLAAGGQLKRIRP
                     AEWLIFGLCSFGPILIWAAARFHYDGMRFFSGMVSYDLMKRSSSVIEGHQGDAFYYVT
                     ELFVNTYYTGLFYLLAFLVVIVAVFGMKSGRIHFSRIRPIWNDLMFYGLWILVPTLLY
                     SIAKSKIKWYVYPLIYPLVIFTGF"
BASE COUNT         1318 a         1287 c         1389 g         1436 t
ORIGIN      
        1 acgataacaa tggaatattt gtctttcaga accgaaatga gctcctcgat tttcatggtg
       61 gaaatgggat caagggcgct ggtcggctca tccattaaaa cgacctccgg ctcaacggcc
      121 agagcccggg cgatgcaaag cctttgctgc tggcctccgg aaaaacccag cgcgtttttt
      181 ttcagcctgt ctttcacttc gtcccagagt gccgcgtcct ccaggctacg ctgcacaatt
      241 tcatcaagct gactgcggct tttgattccg tgaatcctcg gcccgtacgc tacgttgtca
      301 taaaccgaca tgggaaaagg gttgggtttc tggaatacca tgccgacccg tttgcgaagc
      361 agcgtcacgt cggtatcccg ggcgtagata tcctcgccgt ccaatgtgac cttgccttcg
      421 atccggacgc cggcgatcag gtcgttcatc cggttcagcg ttttcaaaaa ggtggacttt
      481 ccgcagccgg agggcccgat cagcgccgtc acccggtttt cgtaggcttc aagattaaca
      541 ttttttaagg catggacatc gccgtaatgt aaattcagat tttctgaagt gattttgatg
      601 ttcatttatt tgcctccctt tcccgaaatt tttctttcca gatgattgcc gatgatccgc
      661 gagccgagac tgaaaacaaa aaccatgagg atcagcacgg cggccgacag gtctgccagc
      721 tggttggcgt tggcgtctat gccttccgtt ttcaatgccc agatgtggac ggcgagtgtg
      781 tcggctggac gaaacggatt caaaggggag gtgacgctca tgggattcca gttgttaaaa
      841 ttgacgtcgg agctcatgcc ggaggtgaac agcagagcgg cggcttcacc gaaacctctt
      901 ccggccgcca taatcacacc ggtcacaata cggggaacaa cggcgggaaa caggacgtta
      961 acaatcgtct gccagtgggt cgcgccgatt ccgaggctgc cctcccgata gattgcggga
     1021 atgccctcca gagcatcttc cgtcacggtg gtcaaaagcg gcagatttaa aatcgagacg
     1081 gcggccgcgc ccgcaagcag attccatttt gatccggtca gaaccagaaa taccagatat
     1141 ccgaacaaac cgacgacgat ggagggcagg gaagagagtg tttcaataca ggttcgcaga
     1201 aaacgggtta atctgccgtt tccggcgtat tcagccatgt aaattcccgc ggcgatgccg
     1261 atcgggaggg aaataaccag agaaatcaag accaaataga tcgtgttaaa aaactcaact
     1321 cctattccgc ccctgttgaa ggcaagatat tccggtttgc aggcgagggc tcccttatac
     1381 aggatataaa gggtgaaagc ggcgagcaaa agcagaaaaa aacaggcgac ggcgtagagt
     1441 acgccggtcg cggccctgtc ggaaaacagt gcgcgcttat gagaaaactt cattttttat
     1501 tttcccccat tcttccgaca attctgatca ggacgataaa gatgaacgaa atgacaagaa
     1561 gaaacagtgc catcgtccag agtgcgtcgt tccattcgct gccggaaatg gtgttgccca
     1621 tgtcggcggt gatttccgac gtcagattta cggtcggctt gagtatattt cccgggaaag
     1681 cctgcatttt tccaattacc atggcgacgg caagcgcttc gccaaaggcc cgggcgaggc
     1741 ccagcaccac gccggtaaga attccgggaa gggcggccgg cacgatcaca tgacggatca
     1801 gctgccatct ggtagagccc aggccgtaag aggcctcctt gtattcttcc gcgacattcc
     1861 gtatggcgtc ggaggaaacg cttgtgatgg ttggaaatat cattacggcc aggacgattg
     1921 ctcccgcgag cacgctgtaa ccgtaaggca ggtgaaacag tttggcgatt gccggcacca
     1981 aaaccgtcag gcctacccag ccgtaaacta ccgacgggat gcccacaaag atttctactg
     2041 cgggctgaag cagttttctg cccagatggg gggatatttc agtcaggaac agcgccgccg
     2101 ccagactgaa gggcgttgcg atcagcaggg cgagcaggct gattgtgatt gagccgaaaa
     2161 taaaaatcgc ggcacccacg ctcccgccct ggccgatctg ggcggggctc catttggagg
     2221 aaaacaaaaa ttcgaggatg ctgtgtttat gaacggtgaa tgtggccatg cctttgtaaa
     2281 acagaaaagc gccgatggac agtgtgagca ggataataaa aaatccaaac agtgtcacga
     2341 cggcccttcc aagatattct ttcctgattt ttcttcgtaa atccatccgg atcaaccttt
     2401 catcatggct tcggattcaa tcatgtattc ggtaagacaa cggattctta ctgttttgta
     2461 tgaccgtatg gagattaggc tgagctttaa agcagaatac ttcaaattac tacggcgcaa
     2521 ggccgggcag cattccgctg ccggcctttt tgccggaggt gatattttta ttttagcaac
     2581 ggtttatttt gcggcgggcg ctgctctttt cactgtcatt tttccgatca gcccgtagcc
     2641 ttttgcggta acggtagagg aaaaactgtc actcttcatg taatcgatca gggcttttgc
     2701 agcgccgctt gcttcgccgt tggtatacat gtgctcatag ccccagattg gatattttcc
     2761 gctgtagaca ttgtcgtacg caggtttgac accgtcgatg ctcagcgcgt tcaccgagct
     2821 gtcgagatag gagaaagcaa ggtaggagat cgagcctttg gtctgcgtga cattctgacg
     2881 gagtgtgccg gagttatctt ccgtcagagc ctggccggtt acttcgtcgt tcccgtcaag
     2941 tccgtatttt ttaaagaggg cgcgtgttcc ggaagaggac ggccggttga tgataacgat
     3001 cgcttcgtcg tttccgccga cttctttcca gttcttaatt tttccggtaa agattttaat
     3061 cagttgatct ttggtgatac tggtgacttt cacatcgggg ttcacaactg ccgcgacgcc
     3121 taccacgcaa acctgatggt cgaccagagt ttttgaggag gaagcgtcaa gcttttcttc
     3181 ggcaaaaaca tccgagtttc cgatgtcgac ggttttattc agagcattct gaagaccctg
     3241 gcctgagccg cctgcgttga ccgtaatgga taaatcgggg tttttcgcct ttaggtcgtc
     3301 ggccgctgcc tgaaccagag gctgcagtgc ggaagatcct gccgctgtga tgcttcccga
     3361 aagcgactgg gatgaggacc cgctctgaga ggcggaagat gcgttatctg aattaccgca
     3421 ggccgtcatt gacagaccta taagagctgt cgccatgaaa agtgccaaag ttttcttcat
     3481 tcagttacac tccttaactt cttttggatt acaactatta ctatactgtc aaccgtagcc
     3541 atcaaaaagc aggctatggt atatgaaatg taaagtttgt gtaaagttcc aattctgtta
     3601 cagttttctt gattatatcg acagaaaaac ccttcggtga tataatttac atactttact
     3661 aaattataag agactgaaag aaaacagcct gctccaaatt ttcgaaaaaa atgcaattac
     3721 cgggcgggca ggaaaagaat ccggcggagg aatttgaaat gaaactgttt tttattgcgg
     3781 atatacacgg ttccgcatat tatctcaaga aaacgcttgc agtttatgaa caggaaaagg
     3841 cggattatct tgtgattttg ggcgatgagc tttaccacgg ggccagaaac ccgcttccgg
     3901 aagaatataa ccccaaagaa gttgcaggtc tgctgaatga ttattccggt aagatcattg
     3961 cggttagggg caattgtgac agtgaagtgg atcaaatggt tctcaattat ccgattatgt
     4021 cggattattc cacaatctta tatcatggaa aaaggctttt tctgacccac ggccatcttt
     4081 atcaggaaaa caatatgccc ggtttgtgcg acggggatgt atttttttac ggccataccc
     4141 atgtgctccg ggccgaaaaa aaggatggtg tgacaatcat taatcccgga tccattacgc
     4201 ttcccaaaga gaatcttcct cataccttcg gtctgctgga gaatgacgtc tttgaaatca
     4261 gggatctaaa cggcggggtt gtcaaaaaaa tcagatttgg gaaggtcctt tgacacgcgg
     4321 ggtgaaatta tgtcggaaaa taaaatgatt aaatggaaag acaaaattcg ttccggagca
     4381 gtttttttct gcctttgggg gctgtcgatt ctgtatctgg gattcaactg cttttatcat
     4441 ctggaccgga cgatggttat gagctacgat gaagcaagac acggtgtcaa cgcttttgaa
     4501 atgatgcgga cgggcaacta tattgtcagc acatttaact atcacaacga ttactggaat
     4561 ctgaagcccc cgatgagttt ctgggcgatt attctggatt tcaagatatt cggcacaaca
     4621 gtgctgggcc ttcgttttta ttctgctctg gctcttttcc tgacgattct gatgatttcc
     4681 gtattccttt taaaacgtta cggaaaaatc gaatgtcttg tcattacaac catgctggcc
     4741 tgctgtttct ccttctatgc cgggcatttc ggccgcaacg gcgacgcgga cgccttgtac
     4801 gggctgttcc ttacggcggg cgcgctggcc ctgttttatg taaaagacaa tcatgccttt
     4861 ctttatctta cgggcgccat gttttcgttg gcgtttctga ccaaaagctg gcacgcgttc
     4921 atccttcttg ccgtcacagc agcgtgtctt gcggccggcg gtcagctgaa aaggatcagg
     4981 ccggctgaat ggctgatttt cggcctgtgc tctttcggcc cgattttgat ttgggccgcc
     5041 gcccggtttc attacgacgg aatgcgattt ttcagcggta tggtgtctta cgacctgatg
     5101 aaaagatctt cctctgtaat tgaaggccat caaggggatg ctttttatta tgttaccgag
     5161 ttatttgtca atacatacta tacaggtctt ttctatctgc tggcgtttct cgtcgtgatt
     5221 gtggcggtct tcggaatgaa aagcggccgt attcattttt cccgtattag gccgatttgg
     5281 aatgacctta tgttttacgg cctgtggatt ttggttccga cgctgctcta ctcgatcgca
     5341 aagtccaaga tcaaatggta tgtttacccg cttatctatc cgcttgtgat ttttaccggc
     5401 ttttgaaata caaaaataat aatccagacg
//
LOCUS       DATALY010000046         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_355750,
            whole genome shotgun sequence.
ACCESSION   DATALY010000046 DATALY010000000
VERSION     DATALY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355750"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..91)
                     /locus_tag="VHR42_01385"
     tRNA            complement(1..91)
                     /locus_tag="VHR42_01385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Ser,seq:cga)
     gene            complement(207..980)
                     /locus_tag="VHR42_01390"
     CDS             complement(207..980)
                     /locus_tag="VHR42_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025871.1"
                     /translation="MGQQRTIRRSHAEHRRLRRRKRAGITAVGALLILAVVIGTGVLL
                     FYAGKSAVQYIKGPEESNAFFEKYIEPVVVEDPKPFSNIKKVDSNWALMTAIWVALNE
                     DENSGRYGYTEDNRTILPVEDIRKSFETYFGDLIQPQYHTFISGKATFEYDAKENCFY
                     IPAIALSNVYLPKVAKVERNQKTVKLTVEYIPGSGWSSDENGKVIKPAPAKVMYYTLS
                     GSRGKYALLSIENVPDSVQKATASMASSSQKSQSAKGKS"
     gene            complement(1000..2244)
                     /locus_tag="VHR42_01395"
     CDS             complement(1000..2244)
                     /locus_tag="VHR42_01395"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX3025872.1"
                     /translation="MTLDQLGKRAKSASPLLALAGTALKNAALMKIADDLESNADRIL
                     TENRRDIDAAKASGMSEALVDRLSLNVSRISAIADGVRKVAALDDPTGKSDGSWTRPN
                     GLRIVKTRVPLGVVGIIYESRPNVTADAASLCLKSGNACILRGGREAIHSNLAIAEIM
                     RAAIRDSGLPEDCVQVVEDTSRESSREMMRLNEYLDVLIPRGGGGLIRSVVENSTVPV
                     IQTGVGNCHVYVDETAELDMGAKIIFNAKASRPSVCNAAETLLVHEKIADAFLPKAKE
                     LLDTKRVELRGCEKTKAILGGDVVPASEDDWRTEYLDYILAVKVVRNIDEAIEHINRY
                     GSGHSEAIVTESYSNAEKFLNRVDAAAVYVNASTRFTDGYEFGFGAEIGISTQKLHAR
                     GPMGLFELTTIKYAIYGMGQTR"
     gene            complement(2234..>2951)
                     /gene="proB"
                     /locus_tag="VHR42_01400"
     CDS             complement(2234..>2951)
                     /gene="proB"
                     /locus_tag="VHR42_01400"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX3025873.1"
                     /translation="RVLADLKNAGREIILVTSGAIGVGVSRLKLRERPKETEMKQAAA
                     AIGQCELMHLYDKLFSEYGHTPAQILMTRIITDHDNTKRNLINTFDTLLKLGVIPIVN
                     ENDSVAIEEIDSEEIAFGGNDKLSAVVATLVGADLLILLSDIEGLYDKDPHLNQDAKL
                     IPYVYDINDEIRAKAGGAGSALGNGGMVTKLEAAELATEAGIDMIIMDGADPCKMYDV
                     FENRHVGTFFPAKRRKTDDA"
BASE COUNT          691 a          830 c          627 g          803 t
ORIGIN      
        1 tggcggagaa ggtgggattc gaacccacga gcctcgttag agactaccgc atttcgagtg
       61 cgggccgtta tgaccacttc gatacttctc catagggcta aactttgatc agaatcactt
      121 gaatattata tcaaaaccac cgacaggtgt caaacagttt attcagcacc cgaatattag
      181 caatatttca agattcagag cctttatcag gacttaccct tggcgctctg cgatttctgt
      241 gaagaggatg ccatagatgc cgtagccttc tgaacagaat ccggaacatt ttcgattgac
      301 agcaacgcgt attttccgcg gctgccggaa agggtgtaat acatcacttt tgcaggagcc
      361 ggtttaataa cctttccgtt ttcatcagaa gaccatcctg atcccggaat atattctacc
      421 gttagcttta cagtcttctg atttctctcc acttttgcca cttttggcag ataaacatta
      481 ctcagggcaa tggccggtat gtagaaacag ttctcttttg cgtcatattc aaaggtcgct
      541 tttccgctga tgaacgtatg gtattggggc tggatcaaat cgccaaagta tgtttcaaaa
      601 ctcttacgga tatcctcaac cggcaatatg gtacgattgt cttcggtgta tccataacgt
      661 ccgctgtttt catcctcatt gagcgcaacc caaattgccg tcatcagcgc ccagttggaa
      721 tccacttttt taatattgct aaatggcttg ggatcttcca caaccaccgg ttcaatatat
      781 ttttcaaaaa acgcattgct ctcttccggg cctttaatat actgaaccgc cgattttccc
      841 gcgtaaaaaa gaagtacacc tgttccgata acgactgcaa gaatcagcag cgctccaacc
      901 gcggtaatcc cggcgcgttt ccggcggcga agccttctat gctctgcgtg agaacgtctg
      961 attgtccttt gctgtcccat cggctatact ccttacccat taccttgtct ggcccatgcc
     1021 gtatatggca tattttatcg ttgtcagttc aaaaagaccc atcgggcccc tcgcatgcag
     1081 tttctgcgtc gatattccaa tctccgcgcc gaagccgaat tcatatccgt ctgtaaaccg
     1141 cgtggacgca ttaacataaa cggccgcggc atccactctg tttaagaatt tttccgcatt
     1201 ggaatagctc tcggtcacaa tggcctcact gtgcccgctg ccataccgat tgatatgctc
     1261 aattgcctcg tcaatattcc ttacaacctt cacggccaga atataatcca gatattcggt
     1321 tcgccagtca tcctcagagg ccggaacaac atcgccccct aaaatggctt tcgtcttttc
     1381 acagccccgc agctccaccc gtttggtatc taaaagctct ttggcctttg gtaaaaacgc
     1441 gtccgcaatt ttctcatgaa ccagcagcgt ttccgccgca ttgcaaaccg atggtctgga
     1501 cgccttcgca ttaaagataa ttttagcgcc catatccagt tccgccgttt catcgacata
     1561 aacatgacag ttccccactc cggtctgaat gaccggaacc gttgaatttt ccacgaccga
     1621 tcggattaat cccccgcctc cccgcggaat cagtacgtct aaatattcgt tcagccgcat
     1681 catttcccgg gaggattccc gtgaggtatc ttccaccacc tgaacgcaat cctccggaag
     1741 tccggaatcg cggatagcgg cgcgcataat ttctgcgatc gcgagattgg agtgtatcgc
     1801 ttccctgccg ccacgaagga tacaggcatt tcccgatttc aggcaaagag aagcggcgtc
     1861 ggccgttaca ttcgggcggg attcataaat gatgccgacg acacccagcg gcactcttgt
     1921 tttgacaata cgcagaccgt tcggccttgt ccaggatcca tccgactttc cggtcggatc
     1981 gtcaagagcc gcaacctttc tcacgccatc ggcgattgct gagattcggc tgacattcag
     2041 ggaaagacgg tccaccaaag cttcgctcat tcccgacgct tttgccgcgt cgatatcccg
     2101 gcggttttcc gtcaagatcc gatccgcatt tgattccaaa tcatcggcga tcttcattaa
     2161 tgcggcattt ttcagcgcgg ttccggctaa agcaagtagg ggcgacgctg attttgccct
     2221 tttccccaat tgatcaagcg tcatcagtct tcctcctttt cgccgggaaa aaggtcccga
     2281 catgcctgtt ttcaaaaaca tcatacatct tacagggatc tgctccatcc ataataatca
     2341 tgtcgattcc ggcctcggtc gccagctctg cagcctcaag cttggttacc atcccgccgt
     2401 ttcccagagc agagcctgct cctccggcct ttgctctaat ttcatcatta atatcatata
     2461 cataaggtat gagcttcgca tcctgattca aatgcggatc cttgtcataa agcccctcga
     2521 tatcactcaa cagaattaaa agatcagccc cgaccagcgt cgctactacg gcggaaagct
     2581 tgtcgttgcc tccgaatgcg atttcctcac tgtctatttc ctcaattgca accgagtcgt
     2641 tttcatttac aattggaatt acgccaagtt ttagaagcgt atcgaacgta ttgattagat
     2701 ttcgcttggt attatcatga tcggtaatga tacgggtcat caggatctgg gccggcgtat
     2761 gcccatattc cgagaaaagc ttatcgtaaa gatgcatcag ctcacactgg ccgattgcgg
     2821 cggccgcctg cttcatttcg gtttctttcg ggcgttcccg cagtttcagt ctggaaacgc
     2881 caaccccgat cgcccccgaa gtcacaagga tgatttccct gccggcattt ttcaggtcgg
     2941 ccagaaccct g
//
LOCUS       DATALY010000047         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_701407,
            whole genome shotgun sequence.
ACCESSION   DATALY010000047 DATALY010000000
VERSION     DATALY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701407"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VHR42_01405"
     CDS             complement(<1..1210)
                     /locus_tag="VHR42_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX3025874.1"
                     /translation="MSSRLFQNFPMLLDGATGTNLMRDGMPQGVCVETWILDHPQVLQ
                     NLQRDYARAGSNAVMAPTFETNRHKLAQYGLEDKVEPLIKDLVALSREAVGDKVLVAG
                     NLSPTGLFIEPLGELTFDELTDLYLEQALALERAGVDYIALETFMSLTEARSALLAAK
                     QTGLPVTVTLTVDQNGRTLSGGSVPACLFILASMGADAVGLNCSTGPDIILRCLKEAA
                     PLLSVPVIAKPNAGLPVEGKTGVYDISPLQFAQKVPDFLAAGISILGGCCGTTPEHIG
                     LMRETIDRLPDPIPADGTCSAGLPLAAGERSLFEIGPDILPSAQTVACEDLAETLGDI
                     SGGPIRIPVHNADDAREFGMNAPFLSAGPVIFSSGDAQALEAALKLYQGRALIEAGAG
                     IGDRALSELAE"
     gene            complement(1287..2981)
                     /gene="argS"
                     /locus_tag="VHR42_01410"
     CDS             complement(1287..2981)
                     /gene="argS"
                     /locus_tag="VHR42_01410"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX3025875.1"
                     /translation="MLNPINTAIEEVRSAIMDAMGKCVAEGLLPPEPIPPFSVEIPTD
                     RSHGDFAANIAMISARAFKMSPRKIAESILSKINLPCGYLKSAEVAGPGFLNFTLSPA
                     WYSSVIDAVQKEGETYGSSDLGRGRKLMVEFVSANPTGPMHMGNARGGALGDCLASLL
                     ERAGFDVTREFYINDAGNQIEKFANSLEARYLQIYKGEDVIPFPEDGYHGEDIAQRAR
                     EFADRNGDRFVNAAAEERKAALVEYGLEENIKKLRNDLEKYRIIYDVWFRESLLHEDG
                     EIAETIELLKTRGFTYEKDGAVWYAATKFGSEKDEVLIRSNGVPTYFAADIAYHRNKF
                     LKRGFDKVINVWGADHHGHVARLKGAMDAVGIDSSRLDIVLMQLVRLTRGGEVVRMSK
                     RTGKAISLDNLLEEVPIDAARFFFNLREANSHLEFDLDLAVEQSSQNPVYYVQYAHAR
                     IWSILKNLEAEGIRPRACTPQELELLSSSEERELIRHLAELPSEIIEAAKAYDPARIT
                     RYAIDLATLFHKFYNAQRVKGEDEHLMQARLNLCICVRDVIKNVLTLLKIESPQKM"
     gene            complement(2953..3414)
                     /locus_tag="VHR42_01415"
     CDS             complement(2953..3414)
                     /locus_tag="VHR42_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEX3025876.1"
                     /translation="MAKDVLINIKGTVISENSPPDIIELTTAGRYSSKDGNYYISYKE
                     SEATGLEGITTTLKVEGESSVTLMRNGAATSRLVLEKGRRHLCHYDTGYGQLMVGVSG
                     CKIKSQLSDLGGDLAFFYTLDINSSLISQNEVFITVKEANKKDVKPYQYSN"
     gene            complement(3458..>4236)
                     /gene="murI"
                     /locus_tag="VHR42_01420"
     CDS             complement(3458..>4236)
                     /gene="murI"
                     /locus_tag="VHR42_01420"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX3025877.1"
                     /translation="GGLTVVKELNKILPCEDIVYFGDTGRLPYGTKGSDTIIKFATQD
                     IRFLLSFDVKAIIIACGTVSSVAFNEACAASSVPVVGVVVPAATAAVQATKTGRIGIL
                     GTNATIRSGSYEKEILRIRPDAEVRGRPCPMFVPLVENGYSNEGNPVSAMIAHEYLDG
                     YFEEKTDTLILGCTHYPLLRSFIRQTVGENITLIDAGRETAQAATRLLEENSLLNPGT
                     EPGKCRFYVSDCSEDFTRLAGVFLEHSVFGYVEKAEIENY"
BASE COUNT          906 a         1128 c         1180 g         1022 t
ORIGIN      
        1 gttcggcgag ctcgctgaga gccctgtctc cgatccctgc tcccgcttca attaaagctc
       61 ttccctgata aagctttaaa gctgcttcga gcgcctgcgc gtctccggaa gagaaaataa
      121 ccgggccggc ggaaaggaac ggcgcattca tgccgaattc cctcgcgtcg tcagcgttgt
      181 ggacggggat gcggattggc ccgccgctga tgtctcctaa tgtttcagcc agatcttcgc
      241 aggcgactgt ttgagccgac ggaagaatat cagggccaat ttcaaaaagg cttctttcac
      301 cggcagccag aggaagcccg gctgagcatg tcccgtccgc agggatcgga tccggcagtc
      361 ggtcgattgt ttcccgcatc agaccgatat gctcgggagt tgtgccgcag cagccgccca
      421 gaatcgagat gccggcggct aaaaagtccg gtactttttg ggcgaactga agaggtgaaa
      481 tgtcatagac accggttttg ccttctaccg gaagcccggc gtttggtttc gcgatgaccg
      541 ggacggacag caaaggtgcc gcttctttga gacagcggag aataatatcc ggcccggtgg
      601 agcagttcag ccctacggcg tccgccccca ttgaggccag tatgaaaaga caggccggaa
      661 cactgccgcc cgaaagggtt cttccgtttt gatcgaccgt cagcgtcacc gtaacaggca
      721 gaccggtctg ctttgccgca agaagggccg aacgggcttc ggtgagactc atgaaggttt
      781 ccagcgcgat gtagtcgacg cctgctctct ccagggccag agcctgttcg aggtagagat
      841 cggtcagctc atcaaaagtc agttcgccca gaggctctat aaatagcccg gtgggggata
      901 ggttgccggc cacaaggacc ttgtcgccca ccgcttcgcg ggaaagggcg acgaggtcct
      961 tgattagggg ttcaacctta tcttccagac catactgtgc cagcttgtgg cggttggttt
     1021 caaaagtcgg cgccatcacg gcgttactcc ccgcgcgggc atagtcccgc tgaagattct
     1081 gcagtacctg aggatggtca agaatccagg tttcaacgca gacgccctgc ggcataccat
     1141 cgcgcatcaa gttggttccg gttgctccgt caagcagcat cggaaaattt tgaaataatc
     1201 ggctggacat atttgcaggc tccgccgctg ccgctgcggc tatttgaaag gcaatattcc
     1261 cctgcggcgg atttgggact tgccggttac attttctggg gagactcgat tttaagaagc
     1321 gtaagcacgt ttttgatcac gtcgcgtacg caaatgcaca ggttaagcct tgcctgcatc
     1381 aggtgttcat cttcgccctt gacgcgctgt gcgttgtaaa acttgtggaa aagcgtcgca
     1441 aggtcgatgg cgtaacgtgt aatgcgggcc gggtcatacg ctttggccgc ttcgataatt
     1501 tcagagggca gctccgcaag gtgacggatc agttcccgtt cttccgaaga gctcaggagc
     1561 tcaagctcct gaggcgtgca ggctctgggc cggatgccct cggcttcaag gttttttaaa
     1621 atactccaga ttcttgcgtg ggcatactgg acgtaataga ccggattctg ggaggactgc
     1681 tcgacggcga gatccaggtc aaattccagg tggctgttgg cttcccggag attgaaaaag
     1741 aagcgggccg cgtcgatagg gacttcctca agcaggttat caagggagat ggctttaccg
     1801 gttcttttgg acatccttac aacttcgccg ccgcgggtca ggcgaaccag ctgcatgaga
     1861 acaatgtcaa gccgggaact gtcgatgccg acggcatcca tagcgccctt gagccttgcc
     1921 acatggccgt gatggtcggc tccccacacg tttatgacct tgtcaaaacc tcttttgagg
     1981 aatttgttgc gatgataggc aatatcggcg gcaaaataag tgggaactcc gttggaacgg
     2041 atgagcactt catctttttc cgagccgaat tttgtggctg cgtaccagac ggcgccatct
     2101 ttttcatagg tgaaacccct ggttttgaga agctcgatgg tttcggcgat ttcgccgtct
     2161 tcatggagaa ggctttcccg gaaccagaca tcatagatga tgcggtattt ctccagatcg
     2221 ttccgcagct tcttaatgtt ttcttcaagc ccgtattcga cgagggcggc tttgcgctcc
     2281 tcggccgcgg cgttgacaaa acggtcgccg ttcctatctg caaattcccg ggctctctgg
     2341 gcgatgtcct cgccgtgata gccgtcttca gggaacggaa tgacgtcttc acccttgtaa
     2401 atctgaagat atctcgcctc gagggaattc gcgaattttt cgatttgatt gccggcgtcg
     2461 ttaatataaa attctctggt gacgtcaaaa ccggcccgtt ccagaaggga tgccaggcag
     2521 tcgcctaaag ctccgccccg ggcgttgccc atatgcatag ggcctgtggg gttggccgat
     2581 acaaattcaa ccatcagctt ccggccgcgg cccagatccg atgagccgta agtttcccct
     2641 tccttctgaa ccgcgtcgat gaccgaggaa taccatgcgg gggaaagcgt gaaattcagg
     2701 aatcccgggc cggcaacctc agcgcttttg agatatccgc agggcagatt gatttttgac
     2761 aaaatcgatt cggcaatttt tctcggggac attttaaaag ccctggcgga gatcattgca
     2821 atgttggcgg caaaatcgcc gtggctgcgg tcggtcggaa tctcaaccga aaacggcggg
     2881 atcggttccg gcggcaacag accctccgca acgcattttc ccattgcgtc catgattgcg
     2941 cttctgactt cttcaattgc tgtattgata gggtttaaca tccttcttat ttgcctcctt
     3001 cacggtgata aagacttcgt tctgactgat caggctgctg ttgatatcaa gcgtatagaa
     3061 aaacgcgagg tctccgccca ggtcgctcag ctgcgatttg attttgcagc cggaaacgcc
     3121 gaccattaac tgaccgtaac cggtgtcata atggcagaga tgtcgtcgcc ctttttccaa
     3181 aacaagacgc gaggtcgcgg cgccgtttct catcagggtc acgctgcttt cgccttcgac
     3241 cttcagcgtg gtagtaatgc cctccagacc ggtggcctcc gactctttgt aggagatata
     3301 gtaattcccg tccttgctgg aatatcgacc ggcggttgtc agctcgatga tatcgggcgg
     3361 ggagttttcc gatattacgg ttcctttaat gttgatcagc acatcttttg ccataagttc
     3421 ctcctgccgg tgactgttgt cacggctccg gttatcgtca ataattttca atctccgctt
     3481 tttccacata gccgaatacc gaatgctcaa gaaacacacc ggccagccgg gtaaaatctt
     3541 cggagcagtc gctgacatag aaccggcatt ttcccggttc ggtgcccgga ttgaggaggc
     3601 tgttttcttc aagcaaccgg gtagctgcct gagcggtttc ccttccggcg tcaatgagcg
     3661 tgatgttttc tcccacagtc tggcggatga agctccgcag caggggataa tgggtgcaac
     3721 ctaatatcag ggtgtcggtt ttttcttcaa aatatccgtc cagatattcg tgcgcgatca
     3781 tggcagacac ggggttgcct tcattggagt aaccgttttc gaccagagga acaaacatcg
     3841 gacacggccg gccccgaact tccgcatcag gccgtatgcg gagaatttct ttttcataag
     3901 aaccgctccg aatggtcgcg ttggtgccga gtatgccgat acggccggtc tttgtggcct
     3961 gaacggcggc tgttgccgcg ggaaccacca cgcccacgac gggcacggat gaagcggcgc
     4021 aggcttcgtt gaaagcaacc gagcttacgg ttccgcaggc aatgataatg gccttgacgt
     4081 caaagctgag cagaaagcga atatcctgag tggcaaattt gataatggtg tcgctgcctt
     4141 tggttccata aggaagccgg ccggtgtctc caaaatagac gatgtcttcg cagggaagta
     4201 tcttattaag ctctttgacg acggtcagac cgccga
//
LOCUS       DATALY010000048         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_183941,
            whole genome shotgun sequence.
ACCESSION   DATALY010000048 DATALY010000000
VERSION     DATALY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183941"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VHR42_01425"
     CDS             complement(<1..399)
                     /locus_tag="VHR42_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX3025878.1"
                     /translation="MKNLSIMIVGVGGQGTLLASRVIGSAAVAAGLDVKVSEVHGMSQ
                     RGGSVETYVKFGDKVHSPLVGKGEADVIICFERLEALRFAAYLKKDGILIVNEQVIDP
                     MPVITGSQKYPERIIEKLIEQLPQGRVVAFD"
     gene            complement(416..2170)
                     /gene="iorA"
                     /locus_tag="VHR42_01430"
     CDS             complement(416..2170)
                     /gene="iorA"
                     /locus_tag="VHR42_01430"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX3025879.1"
                     /translation="MKRLMLGNEAVAHGVRDAGATVVSAYPGTPSTEITENIARFDDV
                     YSEWAPNEKVAAEVAIGASIAGARSFCAMKHVGLNVAADPVFTASYTGVNGGLLITVA
                     DDPGMHSSQNEQDSRYYARSAHLPMLEPSDSQECYDFAREAYRISEEFDTPVVLRLTT
                     RIAHSQSLVEQRERDGFQLKPYVKDFRKYVMMPGMARARHLVVEKRDIDLKKFADETG
                     LNRVEMNDTKIGIICSGIAYQYAKEAFPNASFLKLGMVHPLPEKKIAGFAEKVENLIV
                     VEELEPFFEEVIKRLGIPVLGKDRLTLQGELTPAMLRKNFADLAGTQAEEAAPALSAQ
                     EIPQRPPVMCPGCPHRGAFYVLKKLKLVVSGDIGCYTLGALPPNECMDACVCMGASVG
                     MAHGMEKARGAEFAKNTVAVIGDSTFIHSGITGLIDIVYNKGVSTVIILDNSITGMTG
                     HQDNPTTGFTIKGEPTKQVDLKKLCEAIGVTRVRICDPFDLKAFEQAVKEETAAPEPS
                     VIISQRPCALLKRVKYGSAFEINSEKCKNCGSCLKIGCPAIVRKENGMMINDALCCGC
                     GLCESLCAFGAISRRESR"
     gene            complement(2427..>2766)
                     /locus_tag="VHR42_01435"
     CDS             complement(2427..>2766)
                     /locus_tag="VHR42_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3025880.1"
                     /translation="AFVSVYSYSMNLQTARTAAFLTLVGVQLIHVFECKSESKNLFQI
                     NLFNNFYLIASCIVSFLITLAAVYIPALQPVFGTTSLNLKEIIWVVLYSLIGPVLSIP
                     FVHGKNRRRV"
BASE COUNT          656 a          791 c          653 g          666 t
ORIGIN      
        1 atcaaatgcg actaccctgc cctgcggcag ttgttcgatc agcttttcaa taatcctttc
       61 gggatatttc tggctgcccg taataaccgg catcgggtcg atgacctgtt cattaacaat
      121 caggataccg tcttttttca aataagccgc gaaccgcagc gcttcgagtc tttcaaaaca
      181 aataatgacg tccgcctcgc ctttgccgac caaaggcgaa tgaaccttgt cgccgaattt
      241 cacataggtt tccacactgc cgcccctctg ggacataccg tgaacctcgg ataccttgac
      301 atccaacccc gcggcaaccg cggcgcttcc gatcaccctg ctcgccagca gcgttccctg
      361 gccgccgacg ccgacgatca tgatagaaag atttttcata gtaacctcca tatgattaac
      421 ggctttcccg tctgctgatg gctccgaacg cgcaaaggct ttcgcaaagc ccgcatccgc
      481 agcaaagcgc atcgttgatc atcattccgt tctctttccg gacgatggcg gggcatccga
      541 ttttcaggca gcttccgcaa tttttacatt tttcactgtt gatttcaaag gcagagccgt
      601 atttcactct tttgagcaga gcgcagggtc tttgcgaaat gataaccgac ggctcggggg
      661 cggctgtctc ttcctttaca gcctgctcaa atgccttgag gtcaaacggg tcgcagatcc
      721 gcacgcgggt cacgccgatc gcctcgcaga gtttcttaag atctacctgt ttggtcggtt
      781 cgcccttgat ggtgaaaccg gtggtcgggt tatcctgatg gccggtcatg ccggtaatcg
      841 agttgtcaag aataatgacg gtagaaacgc ccttattata cacgatgtca atcaggccgg
      901 tgatgccgga atgaataaac gtggagtcac cgatcacagc caccgtgttt ttggcaaact
      961 cagcgcctct ggccttctcc atcccgtgcg ccattccaac actcgcgccc atacagacgc
     1021 aggcatccat gcactcattc ggaggcaaag ccccgagcgt atagcagccg atgtcgccgc
     1081 tgaccaccag ctttaatttt ttcaacacat aaaaagcgcc tctgtgaggg cagcctgggc
     1141 acatgaccgg cgggcgctgc ggaatttcct gagcggatag cgccggcgcg gcttcctccg
     1201 cctgcgttcc cgcaagatca gcgaaatttt tccgaagcat ggccggggtc agttcaccct
     1261 gcagggtcag ccggtctttc cccagtaccg ggatacccag cctcttgata acctcttcaa
     1321 aaaacggctc aagttcctca actacgataa gattctctac cttttcggcg aaaccggcaa
     1381 ttttcttttc gggaagagga tggaccattc ccaattttaa aaagctggcg ttgggaaacg
     1441 cttctttcgc atactggtaa gcgatgccgg aacagataat gccaatcttc gtatcgttca
     1501 tctcaacgcg gttcaatccg gtctcatcag cgaatttttt caggtcaata tcccgttttt
     1561 ccacgacgag atgccttgcg cgggccatgc ccggcatcat gacatacttt ctgaaatcct
     1621 tgacataggg ctttaactga aacccgtcgc gttcccgctg ctcgaccaga ctctgggaat
     1681 gggcgatccg ggtcgtcaga cgcagaacga caggggtgtc gaattcctcg ctgattcggt
     1741 atgcttcccg ggcaaaatcg tagcattcct gactgtcaga cggctcgagc atcggaagat
     1801 gcgcgctgcg cgcgtaatag cggctatcct gctcgttctg actggaatgc ataccgggat
     1861 cgtccgccac ggtgatcagc aaaccgccgt taacccccgt gtaggaagcg gtaaaaaccg
     1921 ggtcggctgc aacattcagg ccaacgtgct tcatcgcgca aaatgatctt gctccggcga
     1981 tggacgcacc gattgcaacc tcggccgcaa ccttttcatt cggggcccac tcgctgtaaa
     2041 cgtcgtcaaa acgcgcaata ttctctgtga tttcggtgct cggggttccg ggataagccg
     2101 aaacaaccgt cgctccggcg tcgcgcactc cgtgtgcgac cgcctcattg cccaacatca
     2161 atcgtttcaa tatgatacct ccagaagctt tcaaactaaa tctctatctt gtgataattg
     2221 gtttctcaaa tcaatgaccc gttttgccca gccgaacaac cgcctgataa ttttggcgca
     2281 aaacgattcc cgtttatggg tatgccgtcc tgttacaaaa tcatactgtt ttgaagttta
     2341 ttttatcata tcctgcaact tttttcaacg agaccgacaa aatgtgcggg ccgaggattc
     2401 ggcccgcaca cgacgtaaat ctgcttctat accctccgcc tgttcttccc gtggacaaac
     2461 ggaatagaca aaaccggccc aatcagagaa tatagcacca cccagataat ttctttcaga
     2521 ttcagcgaag tggtcccaaa aaccggctgg agcgccggta tgtaaaccgc agcaagggta
     2581 atcagaaatg aaacgataca ggacgcgatc agataaaagt tgttaaacag attgatctga
     2641 aacaggtttt tgctctcgga tttgcattca aacacatgga tgagctgcac gccgaccaat
     2701 gttaaaaacg ccgcggtccg cgccgtctga agattcatcg aatagctgta aaccgacaca
     2761 aaagcc
//
LOCUS       DATALY010000049         5405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_529138,
            whole genome shotgun sequence.
ACCESSION   DATALY010000049 DATALY010000000
VERSION     DATALY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529138"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VHR42_01440"
     CDS             <1..1057
                     /locus_tag="VHR42_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX3025881.1"
                     /translation="DESPTKYVDAINKIKKTEEPEGKKQKAKKAHAIPILSGIAAALL
                     LIALIVVFIALYANGYFPGSKVPDVQVPNLVGKKYVDVQADTSYKNFTIKQGNTEYSD
                     KYDEGQIISQDPAANETVKKGSTITVTVSKGSQKGTVPDVADSDQSTAQVTLQNQGFT
                     NTKISKIYDDTTAVGYVIKTDPEANSNVSLSATITLYVSMGPDPGALISVPDLTGLTY
                     SQAKSRLEQNNLKIGTETSQYSSSASGTVISQSPTNGTQVSKNTVVNVVVSKGQKPAT
                     ATVTLQDYYGDDASTVRKNLINLGLNVTIKQVKSTVAAGLVISQNPVGGSTVNVGTTV
                     TLYVSYNDQSSGTGGNQ"
     gene            1044..1943
                     /gene="rsgA"
                     /locus_tag="VHR42_01445"
     CDS             1044..1943
                     /gene="rsgA"
                     /locus_tag="VHR42_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX3025882.1"
                     /translation="MEISNMKNEGFIIKATGGFYYVRCGEETYECRARGIFRKEGISP
                     LCGDRVVFSIENNTGTVTEILPRKNELVRPPVSNLDQLIIVISTRDPEPNCLVIDKLT
                     AIAEIKKIEPVIVFTKTDLESPEGLRECYEKAGFEVICVSSANGMGVEKVKAVLKGKL
                     SAFTGNSGVGKSSLLNRIDSRLGLETGEISHKLGRGRHTTRTVQLYQTGDGGWVADTP
                     GFSSLDIERFETIFKDDLQYAFREFGPYWDNCRFNGCSHIREKDCAVMGAVENGEIPA
                     SRYQSYCQLYDQVKGLKEWELKK"
     gene            1925..2611
                     /locus_tag="VHR42_01450"
     CDS             1925..2611
                     /locus_tag="VHR42_01450"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEX3025883.1"
                     /translation="MGTEKMNRCMIIAGGPIGDYGRICSKIRFRAQDYIICVDGGISH
                     AAGLGVSPDLMVGDMDSAARIPAGVKTLRYQSEKDETDTILAIQYALKEGYRDITIVG
                     GLRGRLDHTMANMEALLYLTRNGGSGRIVDSDNEVCLLMEGKMTFSKRENTYFSVFPL
                     FESAEGVTEKGFKYALDRATLKSSFPLGVSNEFKEDQAEISVERGPLLIILSEEIHEK
                     NKTPYNVNGK"
     gene            2724..2867
                     /locus_tag="VHR42_01455"
     CDS             2724..2867
                     /locus_tag="VHR42_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025884.1"
                     /translation="MNPLKCIALAAVFLGVGILITCFLPAQVLVVIAAAAIIIIGCYY
                     LRC"
     gene            3164..4765
                     /locus_tag="VHR42_01460"
     CDS             3164..4765
                     /locus_tag="VHR42_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HEX3025885.1"
                     /translation="MDLSLTRDTVRISEPVFETAADHPIECDIILPDYCPDIARILKS
                     EANVCIDSKSVDGGRLTIGGNFTVKIIYIPENSGVIRCISHDQAFSAQFEAGQAEPEA
                     YVKAKARVVYVNCRPVGPRRVQVKASVGISAKIWCRKEEEFVSGAEDQNAEMLRKQMK
                     ACSLVGAADRDFKLSDELEVGYGKPEIAAVIKSDAVAVVQDYKVISNKIILKGEVMLH
                     TIYSTDQDGGEMREDEEQENTPRPKLEMMEHTIPISQIIDLEGADEDCICDVGLMAGA
                     LKVEAASDEDGESRVLAVEMDLTATVRAYKMQEFFIVTDAYCPDYDMDLKTRQLSFEQ
                     IVDTVKATEMIRESVDIAGMDVASITDCTVKADIDNVSLEGKVLNISGSLNASFLAED
                     SQSGPSGFEKAIPFRIREELKEAAEVMRCEPNITVVSTGYSMNGLDKIDLRVECMVEA
                     MIFGVSSETAVTDMNVDDSRPKECGNKKALTLYYADEGEKIWDIAKKYSTSMDAIKRE
                     NGMEEDRLNARRMLLIPKKKCGRTE"
     gene            4920..5270
                     /locus_tag="VHR42_01465"
                     /pseudo
     CDS             4920..5270
                     /locus_tag="VHR42_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
     gene            complement(5172..5402)
                     /locus_tag="VHR42_01470"
     CDS             complement(5172..5402)
                     /locus_tag="VHR42_01470"
                     /inference="COORDINATES: protein motif:HMM:NF042743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HEX3025886.1"
                     /translation="MFYKVLGETTGLEINGEGDLIETMMGLSKMKKEYDKIKDALDEV
                     NARVIVLNISESARNNISDAVDHTDTEGRVIG"
BASE COUNT         1467 a         1245 c         1479 g         1214 t
ORIGIN      
        1 cgacgaaagc ccgaccaaat atgtcgacgc aatcaataag attaagaaga ccgaggaacc
       61 ggagggcaag aaacaaaagg caaaaaaagc ccatgccatt cctattctgt cgggaatcgc
      121 cgcggcgctg ctcctgattg cgctgattgt tgtgtttata gcactttatg ccaacggtta
      181 tttccccgga agcaaagtgc cggatgttca ggtgccgaat ctggttggta agaaatatgt
      241 cgacgttcag gccgatacca gttacaaaaa ctttaccatc aagcagggca acaccgaata
      301 cagcgacaaa tacgacgaag gccagatcat ctctcaggat cctgcagcca acgaaacggt
      361 aaagaagggt tccaccataa cggtaacggt cagtaagggc agccagaagg gcaccgttcc
      421 ggatgtcgcc gacagcgatc agagcacagc gcaggttacc cttcagaatc agggctttac
      481 caacaccaaa atttccaaaa tctatgacga tacgaccgcc gtcggttatg tgatcaaaac
      541 cgatccggag gctaattcca atgtatccct gagcgcgacc attactcttt atgtcagcat
      601 ggggcccgat ccgggcgcgc tgatctccgt tccggacctg accggcctga cctatagtca
      661 ggcaaagagc aggcttgagc agaataatct gaaaatcgga actgaaacat cccagtattc
      721 cagctcggcg tcgggcacgg tgatctccca gagtcctacc aacggaacgc aggtttccaa
      781 aaacaccgtg gtgaatgttg tcgtcagtaa ggggcagaag ccggcaacag ctacagtaac
      841 attgcaagat tattatggcg atgatgccag tacggttcgt aagaatctga taaatcttgg
      901 cttgaacgta accataaaac aagttaaaag cactgtagca gcaggccttg taatcagtca
      961 aaaccccgtt ggcggttcga cggtgaatgt tggaactacc gtgaccctct atgtgagcta
     1021 taacgatcaa agcagcggaa ccggtggaaa tcagtaatat gaaaaatgaa ggattcatca
     1081 tcaaagctac gggcggcttc tattatgtcc gatgtggcga agagacttat gaatgccgcg
     1141 ccagggggat attccgcaaa gagggaatat cccctctttg cggcgaccgc gtagtgtttt
     1201 ccatcgagaa taacacgggc acggtgaccg aaattctgcc gaggaaaaac gagctggtgc
     1261 gcccgcccgt atccaatctc gatcagctga tcatcgttat ttccacccgc gacccggagc
     1321 cgaactgtct tgtgatcgac aagctgacgg cgatcgccga aatcaagaaa atcgagccgg
     1381 tgatcgtgtt taccaaaacc gatctcgagt cgccggaggg gctgagggaa tgttatgaaa
     1441 aagccggatt tgaggtgatc tgcgtttcca gtgcaaacgg catgggggtc gaaaaggtaa
     1501 aggctgtgct gaaaggcaaa ctcagcgcgt tcaccggaaa ttccggcgtc ggaaaatcca
     1561 gcctgctcaa ccgaattgac agtcggctcg gtctcgaaac aggggaaatc agccacaagc
     1621 tcggccgggg caggcatacg accagaacgg tgcagctcta tcagaccgga gacggagggt
     1681 gggtcgccga tacgcccggc ttctcttcgc ttgacattga gcggtttgaa acgattttta
     1741 aagatgatct gcaatacgca ttccgggaat tcgggcctta ctgggataac tgccggttca
     1801 acggctgctc acatatccgg gaaaaagact gcgccgtgat gggcgcggtc gagaacggag
     1861 aaattccggc gtcgcgttat caaagttact gtcagctcta tgaccaggta aaggggttga
     1921 aggaatggga actgaaaaaa tgaacagatg tatgattatc gcgggcggcc cgatcggaga
     1981 ttacggccgg atttgttcaa aaatccgatt ccgcgcgcag gattatatta tttgcgtgga
     2041 cggcggaatc agccacgcgg ccgggttggg ggtatcaccg gacctgatgg tgggcgatat
     2101 ggattccgcc gcccgaatac cggcgggggt caaaaccttg cgctatcaaa gtgaaaaaga
     2161 tgaaaccgac accattctcg ccatccagta cgcgttgaag gaaggctacc gcgacattac
     2221 gattgtaggc ggcctcaggg gcagactgga tcatacgatg gccaacatgg aagcgctgct
     2281 ttatctcaca cggaacggcg gcagcggccg gattgtggat tccgataacg aggtctgcct
     2341 tctgatggaa ggaaagatga cattttcaaa gcgggaaaat acttattttt cagtctttcc
     2401 tctttttgag tcggccgaag gcgttacgga aaagggattt aagtatgcgc ttgaccgggc
     2461 gacgctgaaa tcttccttcc cgctcggggt cagcaatgag tttaaagaag atcaggctga
     2521 aatttcggtt gagcggggcc ctttgctgat catcctttcg gaggagattc acgaaaaaaa
     2581 taaaacccca tataatgtta atggtaagta agatctacta cctttcacct ccgcgcagaa
     2641 atgcgcagaa ataaagcatt gttctcaccc ggcctgattc agaccggtaa ggccgataca
     2701 gccggagata cggggtggtg tgcgtgaatc ctttaaaatg cattgcgctt gcggccgtat
     2761 ttctcggcgt cggcatactt attacctgtt ttttacccgc tcaggttttg gttgtcattg
     2821 cggcagccgc tatcatcata atcggctgtt actatcttag atgctgaggg aaccggaggt
     2881 ttggcgtatg aaagttgtag tctggaagag tccgaagttt ttaaagggta ttctgcgaat
     2941 gttattcgga attaaaaaag aagaatattg aaattaaatt ttttaccgga aagcgaaggc
     3001 tttccggtaa ttttttgcat aagattgaat tcggacaaat atatataatt gtgaaacaga
     3061 agtggatttt gtggccggac agccgtttaa aaccgggaat gcggcccgcg tgaaagagca
     3121 aagcatggat ctatcagcaa acaggataaa aggagaggat cttatggatt tatcgttaac
     3181 acgggatact gttcgcatca gcgagccggt ttttgaaacg gcggcggatc atccgattga
     3241 atgcgacatc atattgcccg attattgtcc tgatatagca agaattctaa aatcagaggc
     3301 aaacgtctgc attgattcga aatcggtgga cggcggcagg ctgacaatcg gcggtaattt
     3361 taccgtaaag attatttaca tacctgaaaa ttcgggtgtg atccgctgca tttcccacga
     3421 tcaggctttc agcgcacagt tcgaggccgg tcaagcggaa cccgaagcct atgtcaaggc
     3481 gaaagccaga gttgtctacg taaattgccg cccggtggga ccgagaaggg tgcaggtgaa
     3541 ggcatcagtg gggatctcgg cgaaaatctg gtgccggaaa gaggaagagt ttgtttccgg
     3601 ggcagaggat caaaatgccg aaatgctcag aaagcagatg aaggcttgtt ctctggtcgg
     3661 agccgccgac cgtgatttca agctgagcga cgagctggaa gtcggttatg gcaagcccga
     3721 aatcgcggct gtcatcaaat ccgacgctgt cgcagttgtc caggattata aggtaatcag
     3781 caataagatt atcctcaagg gtgaagtaat gcttcacacg atttattcca ccgatcagga
     3841 cggcggggaa atgcgtgagg acgaggagca ggagaacact cccagaccga aacttgaaat
     3901 gatggaacat accattccca tcagtcagat cattgatttg gagggagcgg atgaagactg
     3961 catctgcgac gtcggtctca tggcgggcgc cctgaaagtg gaagcagcgt cggatgaaga
     4021 cggagagagc cgggtgcttg ccgttgaaat ggatctgacc gccacggtac gggcttataa
     4081 aatgcaggaa ttctttattg tgaccgatgc ctactgtccc gattatgata tggatttaaa
     4141 aacaaggcag ctgagttttg agcagattgt ggataccgtc aaagcgaccg aaatgattcg
     4201 ggaatcggtc gatattgcgg ggatggatgt tgcctccatc acagactgca ctgtgaaagc
     4261 cgatattgac aacgtcagtc ttgaaggaaa ggttctgaat atttccggca gtctgaacgc
     4321 ctcgttctta gcggaagatt ctcagtccgg cccatccggt tttgaaaagg cgattccatt
     4381 caggattcgg gaggagctca aggaagcggc ggaagtcatg cgctgcgagc ccaacataac
     4441 cgttgtttcc acgggttaca gcatgaacgg ccttgataag attgaccttc gcgttgaatg
     4501 tatggttgaa gcgatgatct tcggcgtttc cagtgaaacg gcggttaccg atatgaatgt
     4561 ggacgacagc aggccaaagg aatgcggaaa caaaaaggct ctcactcttt attatgccga
     4621 tgaaggcgag aaaatctggg atattgcgaa aaagtacagt acttcgatgg acgccatcaa
     4681 acgtgaaaac ggcatggaag aagatcgtct caatgcccgc cgcatgctgc tgattccgaa
     4741 gaaaaaatgc ggtagaaccg aatagccgga ccggcttttt tatcctggca gccgcaccgc
     4801 tttcccggat tgcaggtaga aaaggaagcg cgctgcgctt cggccggaag acgtttgagg
     4861 tttaacccgc cgtgtgaaac gaaaaccggc atactaatga cctataggag gagaaaagca
     4921 tggataacag cagtatctat caggaaattg cgcagcgcac ggatggcgat atctacatcg
     4981 gcgttgtggg gccggtgcgc accgggaaat ccacctttat caaacgcttt atggaaaacc
     5041 tggtgctgcc caatatctca aacagcttta agaaagagcg cgcgaccgac gagcttccgc
     5101 agagcgccac cgggcggacg attatgacga ccgagccgaa gtttatcccc aatgaggctg
     5161 tggaagtgac gttagccgat aacgcggcct tccgtgtccg tatgatcgac tgcgtcggat
     5221 atattgttcc gagcgctctc ggatatattg agaacgataa cccgcgcatt cacctcgtcg
     5281 agcgcgtctt tgatcttgtc atattctttt ttcatctttg acaaacccat catggtttcg
     5341 atcaggtcgc cctctccgtt gatttcaagc cccgtggttt ctcccagaac cttatagaac
     5401 agctc
//
LOCUS       DATALY010000050         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_443381,
            whole genome shotgun sequence.
ACCESSION   DATALY010000050 DATALY010000000
VERSION     DATALY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443381"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..71)
                     /gene="ffs"
                     /locus_tag="VHR42_01475"
     ncRNA           complement(<1..71)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHR42_01475"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            597..2519
                     /locus_tag="VHR42_01480"
     CDS             597..2519
                     /locus_tag="VHR42_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3025887.1"
                     /translation="MINFKNLLLGKPLKTTDIKNQKLSRLWGLPIMASDAVSSVAYAC
                     EEILLVLVPVIGTVSFHFVPWVTLPIILLLLILVFSYSQIIDHYPNGGGAYVVSKENL
                     GKYPALLAAAALVVDYIMTVAVSISSATAAIASAFPPLDGYKVEVSLLFVAIITLGNL
                     RGLRESSKLFGLPTYIFIGSMGLMIFVGFFKIITGTLHPVVYSAAHIAGLHNSMEGLG
                     IAILLKAFSSGCSALTGVEAVSNAIPNFKEPSQRNAKHILFMLCGVIIFIFGGTTLLE
                     IHLRVLPVTGLTVTSQIAMAIFGNSFMYYIIQIFTAVILILAANTAYNGLPLLMYILA
                     HDNFVPRQFAHRGTKLSFSNGILFIFVIACILIIEFQADTHRLIPLYSIGVFISFTLS
                     QYGMFRRWIKTKDKGWAYKSWINGFGALATLVGLGVVFFNKFHDGAWILLIAIPVIML
                     IMVYVNRHYDKVGKELRLDCFYPYYDKIGHRSSQCIVLVQTINKSLLKSLNYANSISD
                     NITALHVCRHPEHAESLRKQWESLHIPVKLEIVLTPYQDIIKPFDEYIWKRELGLKHG
                     ENLSVIIIKYVSEHWYDVVLHNQTTYFLERILSKHKNVSSVIMPFHYNAAEFQKLCKN
                     PDLGQDEQQDHPNPEQ"
     gene            2573..3544
                     /gene="dusB"
                     /locus_tag="VHR42_01485"
     CDS             2573..3544
                     /gene="dusB"
                     /locus_tag="VHR42_01485"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX3025888.1"
                     /translation="MKIGNAEIKGYTALAPMAGVADHAMRILCHEFGASYLVGEMASA
                     KGLCMSDRKTSELLAVTPGERPMAVQLFGSDPAVMAQAAKKALEYGPDVIDINMGCPA
                     PKVAGNGGGSSLMKDPVLAGRILEAVVHAVNVPVTVKIRKGWDDKSVNAVEIAEIAEA
                     NGAAAITVHGRTREQMYAPPVDLEIIARVKEAVGIPVIGNGDVRTPEGAKKMLEATGC
                     DLVMIGRGALGTPWIFSQTESYLNTGRYEPAPPVRERMNVMLRHIRMLCEFKGEFIGM
                     KEARKHAAWYLKGMIGAAALRNEAGKLSVYRDIEILAEKVIRAGNES"
     gene            complement(3555..3698)
                     /locus_tag="VHR42_01490"
     CDS             complement(3555..3698)
                     /locus_tag="VHR42_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025889.1"
                     /translation="MRIIDNLCSDLKIKSTQRKTKFITEFIKITLAAIAGAAAGAFFF
                     WLF"
     gene            3731..>3991
                     /locus_tag="VHR42_01495"
     CDS             3731..>3991
                     /locus_tag="VHR42_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025890.1"
                     /translation="MVKGMTRRIVEIKETGSGYFEKAIFFVKSDGNSAAEQTLTEEAM
                     RIIDNLCSDLKIKSTQRKTKFITEFIKITLAAIAGAAAGAFFF"
BASE COUNT         1020 a          878 c          974 g         1119 t
ORIGIN      
        1 aaatccgctt aatgctgctc ggttccccgc ctgacatggt tcacggcaca ccgtcgcacg
       61 ggcggcccgg aacctgcatg tcccgttaca ctatccttcc tggaccccgg ttcatcctga
      121 tgccatttta attttaaacg cacatcatta tggtttatta taatacagaa aatcatttta
      181 agcaatattc tttttcctga acggggctct tgaaatactc tttttccaat gataaaatga
      241 aattgccgta aagaggaaaa ttcggaaaaa tccccaagga gtaacaccgg aagattcgat
      301 tataatattt taggaagacg gctgcatcaa cttcgtgggt ttatattttt taagtccgtt
      361 tgttttatta ttaataggaa gaaaattgag gaccatctat tttgtcgggc gatctgcctg
      421 ttttcgcatg cggatgcaat tacttgaaat ttaggacgaa acccggataa tttgtgcata
      481 atctatcaaa taacgatacc cggttcacaa ataatatcct ctttttgttg ttgtttttgc
      541 gaaatttgta tgctaaaata ttgcagttat gacaaaaata agcgcaggtg aactaaatga
      601 ttaattttaa aaacttactt cttggaaaac ctttaaaaac aaccgacata aaaaatcaga
      661 agctgtcacg tttgtggggc ctgccgatta tggccagcga cgcagtttca tccgttgcat
      721 atgcgtgcga agaaattctg ctggttctgg ttccggttat cggaacggtt tctttccatt
      781 ttgtgccttg ggtaactctg cccatcattc tgttgctttt gattctcgtt ttttcttatt
      841 cccagataat cgaccattat cccaacggcg gaggcgctta tgttgtttca aaggaaaatc
      901 tgggtaaata cccggcgctt ctggctgcag ccgccctggt tgtggattac ataatgacgg
      961 tggcggtgag tatttcgtcg gctaccgccg caatagcgtc tgcttttccg cctttggacg
     1021 gatataaagt ggaggtttcc ctgttgtttg tggctatcat tactttaggt aacctgagag
     1081 ggctgcggga atcctcgaaa ctgtttggtc tgcctactta tatttttatt ggctcgatgg
     1141 gcctgatgat ttttgtaggc tttttcaaga ttataaccgg aacccttcat ccggtagtct
     1201 attcggctgc acacatagcc gggcttcata attcaatgga aggcttggga attgcaatct
     1261 tgcttaaagc gttctcgtcg ggctgttccg ctctgacggg cgttgaggct gtcagcaatg
     1321 ccataccgaa tttcaaggag ccatcccagc gaaatgccaa gcacatttta tttatgcttt
     1381 gtggagtcat tatcttcata ttcggcggaa ccaccttgtt ggaaatacac ctcagagtat
     1441 tgccggtgac gggccttacc gttacatccc agattgccat ggcgattttc gggaactcgt
     1501 ttatgtacta tatcattcag atctttaccg ccgttattct gattcttgcg gcaaacactg
     1561 catataacgg ccttcccctt ctgatgtata ttctggcgca tgataatttt gttccgcgtc
     1621 agtttgccca caggggaaca aagctcagct tttccaacgg catcctcttt atttttgtca
     1681 ttgcctgtat cttgatcata gagtttcagg cggatacgca tagattgatt ccgctttatt
     1741 ccatcggcgt gtttatctcc tttacactgt cacagtatgg catgttccga agatggatca
     1801 agaccaaaga caaaggctgg gcttataaat catggattaa tggttttggc gcgctggcca
     1861 cactggttgg cctgggggtt gtgtttttca ataagttcca tgatggcgcg tggattcttc
     1921 tgatcgcgat tccggtgatc atgctgatca tggtctatgt aaaccggcat tacgacaaag
     1981 tcgggaaaga gcttcgtctt gactgctttt acccctatta tgataaaatc ggccatcgtt
     2041 ccagccaatg cattgtgctg gtgcagacga ttaataaatc gcttctgaag tcgctcaatt
     2101 atgcgaactc aatctccgat aatattacgg cgctgcatgt ttgccgccat cccgaacatg
     2161 cggagtcgct tcgcaaacaa tgggaaagtc ttcatatccc ggttaagctt gaaattgtgc
     2221 ttactcccta tcaggatatc ataaagcctt ttgacgaata tatttggaaa agggaactcg
     2281 gtctcaaaca cggtgaaaat ctttcggtta ttattatcaa atatgtttcc gagcactggt
     2341 atgatgtggt tctccataat cagaccacct atttcctcga acggattttg agcaagcata
     2401 aaaacgtttc gtcggtgatt atgcctttcc attataacgc cgcggagttt cagaaattat
     2461 gtaaaaatcc cgatttgggc caggatgagc agcaggatca tccgaatcct gaacaataaa
     2521 aaaacgaaaa acaccggcag ataccggtgt tttttcttat caggagatat gtatgaaaat
     2581 tggcaatgcg gagattaaag gttatacggc gcttgcgcca atggcggggg tagcggatca
     2641 cgcaatgcgt attctctgcc atgaattcgg tgcgtcttat cttgtgggtg agatggcgag
     2701 cgccaaaggg ctttgcatgt ccgaccggaa aacgtccgaa ctattggccg tgacacccgg
     2761 tgagaggcca atggccgtcc aactgtttgg gagtgatcct gcggtaatgg ctcaggccgc
     2821 caaaaaagct ctggaatacg gccccgatgt cattgacatc aatatgggat gccccgcacc
     2881 gaaggttgcc gggaacgggg gcggaagctc tctgatgaaa gatcctgttc tggcgggcag
     2941 gattcttgag gcagttgttc atgccgtcaa cgtgccggtt accgttaaaa tccgcaaggg
     3001 ctgggatgac aaaagcgtaa acgccgtgga aatcgccgag atcgccgaag ccaacggcgc
     3061 cgcggcgatc accgtccacg ggcgcaccag agaacaaatg tacgcccctc cggtggatct
     3121 tgaaattatt gcacgcgtca aagaggcagt gggtattccg gttattggga atggggatgt
     3181 ccgaactccg gaaggagcaa aaaagatgct ggaggcaacc ggctgcgatc tggtgatgat
     3241 cggccgcggc gcattaggaa cgccgtggat tttttcccag accgagagtt atctcaacac
     3301 cggccggtat gagcccgcac cgcctgtcag ggaacggatg aacgtcatgc ttcgccatat
     3361 tcggatgctt tgtgaattca agggtgaatt cattggcatg aaagaagcga gaaaacatgc
     3421 cgcatggtat ctgaaaggta tgattggcgc agcagccctt cgcaatgagg cgggaaagct
     3481 ttccgtttat cgggatattg aaattttggc tgaaaaagtg attcgtgcag gaaacgaaag
     3541 ctgaccgggc gtacctaaaa aagccagaag aaaaaggcgc ccgcggctgc tcctgcaatt
     3601 gctgccagag tgatttttat aaattcggtg atgaatttgg ttttccgctg cgtgctttta
     3661 atttttagat cggagcagag gttgtcaata attctcatgg cctccccggt ctgctggaag
     3721 gatggtctat atggtcaaag ggatgacgag aagaattgtc gagattaagg aaaccgggag
     3781 cggttatttt gaaaaagcga tcttttttgt gaaatcagac ggaaatagcg ctgcagagca
     3841 gacattgacc gaggaggcca tgagaattat tgacaacctc tgctccgatc taaaaattaa
     3901 aagcacgcag cggaaaacca aattcatcac cgaatttata aaaatcactc tggcagcaat
     3961 tgcaggagca gccgcgggcg cctttttctt c
//
LOCUS       DATALY010000051        17517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_529532,
            whole genome shotgun sequence.
ACCESSION   DATALY010000051 DATALY010000000
VERSION     DATALY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529532"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VHR42_01500"
     CDS             <1..730
                     /locus_tag="VHR42_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX3025891.1"
                     /translation="DILSFRTIRIALCDDEPISIEKLAAALHRYEERNGVCFQILTFD
                     NPINLLSQLETDQEMMDIALLDIQIGNNNGIKTAKQIHDLNENVIIIFVSNYDSYMQA
                     GYEVNAFRYILKDQIDSYLEKNISAAIHELMKKEKQVFVYKWKGESIRVKFSEIIFFE
                     SCQRLIRIVTAHNESCFYGKLDNIEKELNDLRFIRCHKSFLINVEYIESTSNISARLK
                     GEYSIPISRSKLSAVKRAQVWAMR"
     gene            736..2049
                     /locus_tag="VHR42_01505"
     CDS             736..2049
                     /locus_tag="VHR42_01505"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEX3025892.1"
                     /translation="MVFEWVSVFSYFTECVIIFAFLSFVLPSRDRFERQKILKFLLFF
                     LTFVGIVVINKCPQNPIPIFFIGIVLQTAVSLVVFEGPVFYKVLSVLTFYAALSASDL
                     ITFCIVDLLVKVSAEVLTSQLVPYITSFCISKVFLIFILSLICRFKTNRTDSIKLHNW
                     LLMIVIPISSIVCLFSIMYFSLLLNPKGNSLVLAASASAGILIINASVFYIMEMVSES
                     NRIQTQYLLMKQNTAAQYKRFREIEDIYEQTRIFKHDLDKHLSCISSLVEQNNYEEVH
                     QYLNGLKSAAKLITIPILSGNPIIDTIINTKYNVAKQRGIQMQITACNVNDVPMEQID
                     LCAIVANLLDNAIESCEKITDEKIEKFVKINLMDKNEGLLLSVSNSVEVKPDIRAGRF
                     VSTKSDAEHHGIGLKSVRQSVEKYHGIFETGFDESRFSAVVFLKY"
     gene            2151..2804
                     /locus_tag="VHR42_01510"
     CDS             2151..2804
                     /locus_tag="VHR42_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEX3025893.1"
                     /translation="MLNAFACKIASFFINNSKINAKEKAIFLFGLEIILSTIASIITV
                     LVISIPFGQFWGTTLYLFVFILIRQFTGGYHADSYRSCYLTFLVIYLLTIPIPLLCDS
                     KARILTILFSWISTIPVFLFAPVQNANRMLTAAETIRFRRISRITVCALSILLTAGAV
                     FLNGSKGLFLWGSLALLTMAIFIVVAIIKNGGFKNEKHGNETDERMRGNGHGCSGNG"
     gene            complement(3050..5398)
                     /gene="secA2"
                     /locus_tag="VHR42_01515"
     CDS             complement(3050..5398)
                     /gene="secA2"
                     /locus_tag="VHR42_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEX3025894.1"
                     /translation="MNKRNIENNLKVYGKILEEIKEHSLSDYEEAQLKTLSRQLRARA
                     LDGEPEQQLLPEVFAVVFEVVKRVLGITPFDVQLLAAAAMASGRIAELPTGEGKTLCA
                     VFVACLGALSGKGVHVLTFNDYLAKRDAVWMKPVFDFWGLSVQSIHEGMDKSERKKAY
                     QADITYLTAKEAGFDYLRSFLAGDMDSIVQRPYHMAIVDEADSILIDEARIPLVIAGD
                     MPARVEMKKRILSSVSKLQKNTHFTTEEYSNSLSLTERGVTFLEDQLDVDNLYDPKNL
                     ELLSKINVSMQAVFLLKKDIGYIVRGEQVLLVDEFTGRISKNRQWSDGLQDAVELKEG
                     LAPQTHGIVMNRITLQNFLQFYPFLCGMTGTACPSASEFYEFYEKTVTVIPPHKPCIR
                     IDHPDLIFTHKDAKHQALVKEIEKVHALGRPVLVGTATVEESEYLAKLLRPYLPGITV
                     LNARNDAEEAGIIANAGKRGAVTISTNMAGRGVDIRLGGGDTMEYQEVCALGGLYVIG
                     TNRYESLRIDQQLRGRAGRQGDPGESRFFISLEDDLLEKYGVTESIPPKYRDRKQIEP
                     LKISAVNKAVLHVQRVVEGQALSAKITLSKYSSVAEDQRKIVHQKRMKILAGEETLSV
                     LEKENPAKLKEILKQVSISEYQNAQRQVELFAVNQCWADHLLALESAMDEVQVISQVR
                     GDPFLIYNQKLVENFDHLQQHVSELILNLFDRIVIHNGHIDLDKMGIRGPSSTRTYLI
                     DDGSEQLGMLNGIAASLINAPLFGLYLFTAFWDKLRKKSNES"
     gene            5662..5799
                     /locus_tag="VHR42_01520"
     CDS             5662..5799
                     /locus_tag="VHR42_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025895.1"
                     /translation="MPKDRNKNSQKSKGQNPGYSNDQLGENSGEGRMMKDQEKKEKKK
                     S"
     gene            6113..6772
                     /locus_tag="VHR42_01525"
     CDS             6113..6772
                     /locus_tag="VHR42_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002824366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3025896.1"
                     /translation="MFHLDLLLRLVVACICGSIIGYERKNRLKEAGIRTHIIVALGAA
                     LIMIISKYGSFDVMNIKGISYDPTRIAAQIVSGIGFLGAGMIFIRRQAVSGLTTAAGI
                     WTTAGVGMAVGARMYFVGISATILVLIVQIILHKNFRWLSLPVSEQINIEFDESNDSI
                     SLIQQKLTENNIEIINLKAEKLGNGKMEAELYVKLPQRYEVAKLMDLFKDNPQIHSIE
                     Y"
     gene            complement(6977..7471)
                     /locus_tag="VHR42_01530"
     CDS             complement(6977..7471)
                     /locus_tag="VHR42_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HEX3025897.1"
                     /translation="MPRPKIKLPYSQPEKLLQILSLFGVLGLIVLTVFSMINLPESIP
                     THFGADGRADGWGGKGSLLMLPIMAVILYTALTILERFPWIYNYPGEITEENAEYQYK
                     LGRLALEWIKFIMITIFLYIQWQTAQAARGLSDGLGSWFLPVFLLALFGVTGFMIYKM
                     VKHQ"
     gene            7655..8107
                     /locus_tag="VHR42_01535"
     CDS             7655..8107
                     /locus_tag="VHR42_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX3025898.1"
                     /translation="MQKQTGVSEFLSMHHIRPSYQRVKIMQYLLDRKSHPTIEEIYRA
                     LIPDIPTLSKTTVYNTLNLFIRERITQPLSIEENQTRYDADISVHGHFRCLNCGKIYD
                     FCVDAENLAVSGLDGFQIDSRYIFYQGICPVCRKAEGRSSAEGKPEHQ"
     gene            8132..8668
                     /locus_tag="VHR42_01540"
     CDS             8132..8668
                     /locus_tag="VHR42_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX3025899.1"
                     /translation="MKDLKGTKTEQNLQAAFAGESQARNKYTYFASAAKKEGFEQIAG
                     IFQETADNEKEHAKIWFKCLNGIGSTVENLKAAAAGENYEWTTMYKNFAAQAHEEGFA
                     EIAALFEMVADIEKSHDERYQSLLKNIEDGKVYLKDGSVIWKCRNCGHIHVGKNAPEI
                     CPVCKHPKAFFEVAADNY"
     gene            complement(8783..9484)
                     /locus_tag="VHR42_01545"
     CDS             complement(8783..9484)
                     /locus_tag="VHR42_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025900.1"
                     /translation="MNFLFKILRRIRFPALIFGILLGCAITAFLYIKLPRYTVKKGPD
                     TQGATAVNLLKVENVTQSFRAEENGFSAVKVKLLVQPKELSNMNRIKFELFDEKGLVL
                     SSQIIESSSIVDNQYVEFPISYIENSQGRLYSIKLSPVTAISKGGILLFSGTPNKHAV
                     HFALNGRKAGGELVFDTVYKTNRLEALYNGRYQSSRLNAVISVSVIGIFLGSILLCSL
                     LFHFEGNQSGLNRKA"
     gene            complement(9504..10817)
                     /locus_tag="VHR42_01550"
     CDS             complement(9504..10817)
                     /locus_tag="VHR42_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3025901.1"
                     /translation="MKKTVLDSEKRFVLLLVLASFVLYLSWSLITPFGQAPDEVLRFD
                     IVDFIYKYRQLPVAGDPRLLYGGYGATYAAMPFLSYMVSAFFCIFAKAIGIHLPAYII
                     SRQVSVLSGVITVFFTYKIGAKLFRKSFARYFLPVFLAFLPQFSFANSYTNQDSFMMM
                     LSAVTVYLWIRGIETQWDLKTVVLTGVVSGLMLLTYLNGYVLVVATLLIVLLTHPQKR
                     SPEFFKKLLLCIGILLLISGWFFIRNAYYYHGDIFGLSTTTKISETKAAMGYKPSELI
                     SKLSIRHGLGGLLVFTNWPSTSFVSFWADFGNMEITLNPYYYLAIFALMLISFIGLSY
                     RFLQKKREGIASLFSNKLAITFLFVIFGAFALHVYYSLNCDFQPQGRYLFPAIIPIVI
                     LMTKGFEQFFSQKRIRLFYKVVAASFILLDFYTLYLILFQRYFVL"
     gene            complement(10889..12022)
                     /gene="rffA"
                     /locus_tag="VHR42_01555"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(10889..12022)
                     /gene="rffA"
                     /locus_tag="VHR42_01555"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HEX3025902.1"
                     /translation="MIPFNLPPYAGGELDYIEKAVKVNRKISGDGPFTRSCSDIISKQ
                     LGVRNTYLTTSGTHALEMSALLLGLKPGDEVIMPSYTFVSTANAFLLRGAIPVFVDIR
                     PDTMNINEKLIEDAITPKTKAIVVVHYAGVSCEMDEICEIARKNNLVVIEDAAQAFLS
                     TYKGRCLGTIGSLGAFSFHETKNISMGEGGAIVFNSDAYKLKAEIIREKGTDRSRFFR
                     GEVDKYTWMDIGSSYLPSEINAAYLNAELDKVDDILSDRMNSWNYYYEALKPLEERGI
                     LELPYIPKECTHNAHLFYIKCKDLPTRTALMSHLKKIGIESVFHYIPLHSSPAGKKYA
                     RFHGKDEFTTKESERLLRLPLYYRLGRNQSERVTEEILKFFCS"
     gene            complement(12056..12394)
                     /locus_tag="VHR42_01560"
     CDS             complement(12056..12394)
                     /locus_tag="VHR42_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3025903.1"
                     /translation="MIKYVLLMVFSVAIASVSQVMLKTSSNRVHQSFLKEYCNVLVIS
                     GYGLLALSTVLTLLAFRGLEFKNAPVLESMGYVFILILSRLFLKEKITGRKLLGNLII
                     LAGILIFYLK"
     gene            complement(12387..12764)
                     /locus_tag="VHR42_01565"
     CDS             complement(12387..12764)
                     /locus_tag="VHR42_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3025904.1"
                     /translation="MNGKNNKKSFLLNILFVQLIMILYSLASVTAKFASAYPFTSAGF
                     ILLYALEIFLLGIYAVLWLQVLKKVDLSIAYANRGTAIFWAMIWSALLFHETVTFQNL
                     VGIIVVFCGITIVNSDKCGYKHD"
     gene            complement(12748..13920)
                     /locus_tag="VHR42_01570"
     CDS             complement(12748..13920)
                     /locus_tag="VHR42_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX3025905.1"
                     /translation="MKHAIVIIGANNFQNPLILKAKELGYETHVFAWKDGAVGAETAD
                     HFYPISIINKEEILEICRKISPKGILSVGSDLAVPTVNYIAEKLGLVGNGAECTLMST
                     NKFEMRRRLRKYGLPMPSFTLTGSKDGIDTKGFHYPLIVKPVDRSGSRGVTKVLGPDG
                     LARAIQCSLDSSFEKKAIVEEYIDGPEYSAECISYQGEHRLLALTQKFTTGSPHFIET
                     GHLQPVALDEQTVSKIVTTIQKGLDALGIKFGASHSEFRIKPDGSIAIIEIGSRMGGD
                     CIGSDLVRLSTGYDFIKMAIDVACGNSPDFTKYNQPKIAFIRFIFDENDLYQLNRIKK
                     VCPSAIYRISPIELPGLQKVTDSSTRLGYYILTCSSKKQLNELLQLHEVSCDERKE"
     gene            complement(13986..14981)
                     /locus_tag="VHR42_01575"
     CDS             complement(13986..14981)
                     /locus_tag="VHR42_01575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3025906.1"
                     /translation="MTKVSFVIPVYNSEKTIQNVIGRIIKTINGMPCKLNFEIILVDD
                     GSKDHSVEVCSELTRKVPQVKLFKLSKNFGQASALMAGLNQTSGDFVVCLDDDLQTPP
                     EEFPKLYNKMAEGNFDVVYGFYEHKQHSAFRNFGTKMNELMQAFVLETRSNIKTSSYF
                     IARRYVVDIVRNYNKPFPYLPGLFLQATGNIACVPIHHNSRKNGHSGYGIKQLLGLWL
                     NGFTNFSIKPLRLASIMGVLMSFCAFIMLIVIIIFKLTHANIQEGWTSTITLVLFIGG
                     IQLFSVGLLGEYVGRIYLSVNKTPQFVIRDLTQKDDRPTQDDTESMKRPPSEDQQ"
     gene            complement(15353..15955)
                     /locus_tag="VHR42_01580"
     CDS             complement(15353..15955)
                     /locus_tag="VHR42_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HEX3025907.1"
                     /translation="MTIFYKYGGKLYVNVTNRCPCNCIFCIRQNGDSLGDADSLWLER
                     EPSLEEIIAAYENYDKTGCPEVVFCGYGEPLERLDIVLETCKYLRGVSDMKIRINTNG
                     LSDLINGKPTAHLLKGLADTVSISLNAATAKEYSRVTQPSFGESAFDAMLKFAEDCKQ
                     IVPRVVFSVVDIISPEEIAACQRLADQMGIPLRVRAYEGS"
     gene            16278..17129
                     /locus_tag="VHR42_01585"
     CDS             16278..17129
                     /locus_tag="VHR42_01585"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEX3025908.1"
                     /translation="MNQSFASVSRVAAINDISGFGRCSLTVAIPILSAMGIQCCPLPT
                     AVLSQHTGFEHFYFNDLTPQMSAYIESWKLQGLRFDTVYSGFLGSEEQIRLVDGFITA
                     QKENALIFVDPVMGDGGKVYPTYTSQMCDGMRELVHKANIITPNITEACLLTGTPYPG
                     EAPGIAAAKEMAVKLAELGPAYSVITGICRGNQITSLAYDGNEKNFFTCSVERTDVPY
                     LGTGDIFASVLCGCMTKGQSLRSALQNACDFVRDTTQYTKKLGSPIMDGVAFEPLLFH
                     LGGNMDA"
     gene            17122..>17517
                     /locus_tag="VHR42_01590"
     CDS             17122..>17517
                     /locus_tag="VHR42_01590"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HEX3025909.1"
                     /translation="MHKNNIEKLTFTAVFIALIFVTTAYILHIPVGNGYIHLGDSLIY
                     LAASFLPAPFAIAAAALGAGMSDALTGFPQYVLFTMLIKPLNALCFSQKGDKLLTTRN
                     RIAPFISAPITVVGYYLADVIHYIQTDCRF"
BASE COUNT         4882 a         3841 c         3962 g         4832 t
ORIGIN      
        1 tgacatttta agctttagga caattcgtat tgccctgtgt gatgatgaac cgatcagcat
       61 cgagaagctt gctgccgcac ttcatcgcta tgaagaacgg aatggcgtct gttttcagat
      121 cctgaccttt gataatccga tcaatttgct cagtcagctg gaaacggatc aagagatgat
      181 ggatattgcg ctgcttgata tacaaatagg aaataacaat ggaatcaaaa cagcgaaaca
      241 gattcatgac cttaatgaga acgttatcat tatttttgta tcgaattatg attcctatat
      301 gcaggcaggc tatgaagtca acgcgtttcg ctatattttg aaagaccaga ttgattccta
      361 tctggaaaag aatatctcgg ctgccattca tgaacttatg aaaaaggaaa aacaggtttt
      421 tgtttataaa tggaaaggtg aaagtataag agtaaaattt tctgagatta tcttttttga
      481 aagctgccaa agactgatcc gaattgttac tgcgcataat gaaagctgtt tttacggcaa
      541 actggataat attgaaaaag agctgaacga cttgcggttc attcgctgtc ataaaagctt
      601 cctgatcaat gttgaatata tagaaagcac atcaaacatc agcgccaggc tcaagggaga
      661 atacagtatt ccaatcagcc gaagcaagct gagtgctgtt aaaagggcgc aggtttgggc
      721 gatgaggtag ttgaaatggt tttcgagtgg gttagtgttt tttcctattt taccgaatgc
      781 gtcatcatct ttgcgttttt gtcttttgtt ttaccgagtc gagacagatt tgaaaggcag
      841 aaaattttaa aatttctgct gttcttttta acatttgtag gtattgttgt cattaataag
      901 tgtccccaaa accccattcc gatctttttt atcgggattg ttttgcagac cgccgtgtcg
      961 ctcgttgttt ttgaggggcc ggtattctac aaggttctat ccgtactgac attttacgcg
     1021 gcgttatcgg cttctgattt gattacattc tgtattgtgg atttgctggt aaaagtcagt
     1081 gctgaagtgc tgaccagcca gctggttccc tatattactt cattctgcat ttcaaaagtt
     1141 tttctcatat ttattttatc tctgatctgc aggttcaaaa cgaatcgaac ggattccatc
     1201 aaactgcata actggttgct gatgattgtt attccgattt catcgattgt ctgccttttc
     1261 agtattatgt atttcagcct gctgttaaat ccgaaaggta attctttggt tttggcggct
     1321 tccgcgtcgg ccggtattct tattatcaac gcatccgtat tttatatcat ggaaatggtt
     1381 tcggaaagca accgcatcca gacccagtat ttattgatga agcagaatac cgccgcgcag
     1441 tataaacgct ttcgggaaat cgaggatatt tacgagcaga cgaggatctt taaacatgac
     1501 ctggataaac atctgtcgtg tatcagcagt ttggtggagc aaaataatta tgaagaggtt
     1561 catcagtatc tgaatggatt gaaaagtgcg gcaaagctta ttaccatacc gatattaagc
     1621 ggaaatccga ttattgatac tattatcaac acgaagtaca acgttgcaaa acagcggggg
     1681 atacagatgc agataaccgc ttgcaatgtt aatgatgttc caatggagca gattgactta
     1741 tgcgctattg tggccaacct gctggataac gccattgagt cctgcgaaaa aatcacagat
     1801 gaaaagatcg agaagtttgt taaaatcaat ctcatggaca agaacgaagg gcttcttctt
     1861 tcagtttcga actcagttga ggtgaagccg gatatcaggg ccggtcggtt tgtgagtacc
     1921 aaatcggacg cggaacatca tgggatcggt ttaaaaagcg ttcgtcagtc tgtggaaaaa
     1981 tatcatggaa tcttcgaaac gggttttgat gaaagcaggt tttcggctgt agtattcttg
     2041 aaatattagc aaattattac aataaaaatg tcaaaaatta catagctatt taattgtaaa
     2101 tcaattggtg ctatcttaaa agtcaaagaa gtaagacgtg tggtgagaac atgttgaatg
     2161 cctttgcctg taagatagca tcttttttta tcaataattc gaaaataaat gcgaaggaaa
     2221 aagcaatttt tcttttcggt ctggaaatta tcctgtcgac aattgccagt attattacgg
     2281 ttcttgtgat ttcaattccg ttcggccagt tttggggcac cactttgtat ctgttcgttt
     2341 ttatcttgat ccggcaattt acaggaggat atcatgccga ttcctatcga agctgttatc
     2401 taacatttct tgttatctat ctgctgacca ttccgatccc gcttctgtgt gattccaagg
     2461 cgcgaatatt gacgattctg ttttcgtgga tcagcacgat acctgttttc ctgtttgcgc
     2521 cggttcaaaa cgcaaaccgt atgttaacgg ctgccgaaac gatccgattt cgcaggatca
     2581 gcagaatcac ggtctgtgcg ctgtcaatcc tgctgacagc aggtgcggtt ttcctgaatg
     2641 gatcgaaggg cctgtttttg tggggctctc ttgcattgct tacaatggca atctttattg
     2701 tcgttgcaat aataaaaaat ggaggattta aaaatgaaaa gcatggcaat gaaactgatg
     2761 aacgtatgcg cggtaatggt catgggtgta gcggtaatgg gtaaatggca gccaagtctt
     2821 tttatttttc atcagccgga ggccccgaag gaattacagg agtattcctt caagagtttg
     2881 aaaaagtatc tctaatttag ttcggagttc ttacagcaat gtcacaggag gcctaatttc
     2941 gctttcttct ttgttggtac gtctctgaaa catagcataa tcggtggagg acgcaaataa
     3001 catatttgcg tcctccaccg atttattcta ctttaaggct gaatcccttt taagattcat
     3061 tgcttttctt ccgaagtttg tcccaaaaag cggtgaacag ataaagtcca aacaatggag
     3121 cgttaatgag gcttgccgcg attccgttca acattcccag ctgttcagag ccgtcatcga
     3181 tcagataggt tcttgtggag gatggacctc gaattcccat tttgtctaaa tcaatatggc
     3241 cattgtgaat aacgatccgg tcaaaaagat tgagaatcaa ttcggaaaca tgctgctgca
     3301 aatgatcaaa gttttccacc agtttctgat tataaatcaa aaacgggtcg ccccgcacct
     3361 gactgatgac ctgtacctcg tccattgcgc tctcaagggc cagcaggtgg tccgcccagc
     3421 actgattgac ggcgaaaagt tctacctgcc gctgagcgtt ctgatactca ctaatcgata
     3481 cctgttttaa aatttctttc agttttgccg gattctcctt ttccagaaca gagagggttt
     3541 cttcgccggc cagaatcttc attcgttttt gatggacgat ttttcgctgg tcctccgcta
     3601 cggaggagta ttttgacagg gtgatttttg cgctcagagc ctgaccctca acgactcttt
     3661 gaacatgcag aacagctttg ttaacggctg aaattttgag aggctctatt tgctttctat
     3721 ccctgtattt tggagggata gactcggtga cgccatattt ctcaagcaaa tcatcctcca
     3781 gactgataaa aaaccggctt tcgcccgggt cgccctgcct tccggccctg ccccgtaatt
     3841 gctggtcgat tcgaagactt tcatagcggt tggtcccgat gacataaagc ccgcccagag
     3901 cacagacttc ctgatattcc attgtgtccc cgccgcccag gcggatgtcc actccgcgcc
     3961 cggccatatt ggtcgatatt gtcactgcgc cccgtttccc cgcatttgcg atgattccgg
     4021 cttcctccgc gtcgtttctg gcattgagaa ccgtgatccc gggaagataa ggccggagca
     4081 gttttgccag atattccgac tcttccacgg ttgctgttcc tacaagaacc ggtctgccca
     4141 aagcatgcac cttttctatt tctttcacca gagcttggtg ttttgcgtcc ttgtgtgtaa
     4201 aaatcaggtc ggggtgatct atgcggatac agggcttgtg cgggggaata acagtgacgg
     4261 ttttttcgta aaattcataa aattcggatg ccgacgggca ggcggtgccg gtcatgccgc
     4321 agagaaaagg gtagaactgc agaaaattct gaagcgtgat ccgattcatc acgatcccgt
     4381 gtgtctgcgg agcaagccct tctttcagct ccaccgcgtc ctgcaggccg tcggaccact
     4441 gacggttttt tgaaattcgg ccggtaaact catccaccag cagcacttgt tccccacgga
     4501 caatatagcc aatatctttt ttgagaagga acacagcctg catgctaacg ttgattttcg
     4561 aaaggagctc aagattcttc gggtcgtata aattatcgac atccagctga tcctcaagaa
     4621 aagtgacgcc gcgctcggtt aaggatagac tgtttgaata ttcttccgtt gtaaaatgtg
     4681 tgtttttctg caacttggat actgaggata aaatcctttt tttcatctca acccgggcgg
     4741 gcatatctcc tgcgatcacc agggggatac gcgcttcgtc gatcaggatt gaatcagctt
     4801 catctacgat ggccatatga taaggccttt ggacaattga atccatatcg ccggctaaaa
     4861 aacttcgcag ataatcaaac ccagcctctt tggctgtaag ataggtgatg tccgcctgat
     4921 aggccttttt gcgctcgctt ttatccatgc cctcatgaat cgattgaacc gacaaccccc
     4981 aaaaatcaaa aaccggcttc atccaaacgg cgtctctttt tgcaagataa tcgttaaagg
     5041 ttaaaacgtg cacgcccttg ccggacagcg cccccaagca ggccacaaaa acggcgcaca
     5101 acgtttttcc ttctcctgtc ggcaactctg cgattcgccc tgacgccata gcggcggcgg
     5161 ccagaagctg aacgtcaaag ggagtaatgc caaggacgcg tttcacgact tcaaaaacga
     5221 cggcaaagac ttcgggaagc agctgctgct ctggttctcc atcgagggcc cgggcacgca
     5281 attggcgtga cagggttttt aattgggctt cctcgtaatc ggatagcgaa tgttctttga
     5341 tttcctcgag aattttgccg taaactttta aattgttttc aatatttctc ttattcataa
     5401 tatcaaccgt tgcctcaaag ccggtttggc tcagagactt tctttccgat aaataatctt
     5461 aattacagca taccgttgtt ttccaatttg tcaaccgggg gggcctgtcc gcagcccccg
     5521 gaatcttttt cgtgacgtct tgccggcgca gttgagacag gcagatctta tggtcaagaa
     5581 acctgaccga aaactcgtat aaatgagtcc tttccctgac agaataatga tgaaatgatc
     5641 tattgggaaa ggagaacagc aatgccgaag gacagaaata aaaacagcca aaaatcaaaa
     5701 ggccaaaatc cgggatatag caatgaccag cttggtgaaa attccggtga aggccgcatg
     5761 atgaaggatc aggagaaaaa agaaaagaaa aagtcatagc cagtcataag gagtgctgtt
     5821 ttttgtccct ggacggcgat gccgtatcag ggactttttt tgaagtcata gcatgcggta
     5881 taatgataga aagcggtttc ttttctatta aaacagcccg cgcggccttg agatttgccg
     5941 gcaaatagga taaaataggg agataacatc cggcccaggt aattttaggg atgtttttcg
     6001 gccttggtgt tgagctgtgt gtctgaaatg atgggtttcg gcttcttata aattagaagg
     6061 atattgttga ttttaaaata gggaaggagc atccgcattg agcaaatttt taatgtttca
     6121 ccttgattta cttctcagat tagtcgttgc atgtatctgc gggtcaatca ttggttatga
     6181 aagaaaaaac agactgaaag aggcggggat aagaactcat attattgttg ctctgggagc
     6241 cgctttgatt atgattattt ccaagtacgg ttcttttgac gtgatgaaca ttaagggtat
     6301 atcgtacgac cctacaagga ttgcggcaca gattgtcagc ggaatcggct ttctcggcgc
     6361 aggaatgatc tttatcagaa ggcaggcggt aagcgggctg accaccgcag cgggaatatg
     6421 gacaacggcg ggcgtcggaa tggccgtcgg agccagaatg tattttgttg gaatatccgc
     6481 aacaattttg gtccttatcg tacagattat attacataaa aatttcagat ggctttcact
     6541 gcctgtttca gagcagatca acattgagtt tgacgagagc aatgattcca tatcccttat
     6601 tcaacagaag ctcacggaaa acaatatcga gattatcaat ttaaaagccg aaaaactggg
     6661 aaacggaaag atggaggccg agttatatgt aaaactgcct cagagatatg aagttgcgaa
     6721 actgatggat ctgtttaaag ataatccaca gattcactcg attgagtatt gatttaaagg
     6781 cggatcgagg tttattgtaa cgattattgg tattaatgat tggacgcagg gctgttgtag
     6841 gatgaataaa tggcctgtaa gcatcaaaat gcgctttgag aaggccgttt aagcccaatc
     6901 aacgcgcatt ctttgtgctt tttacagggc tgttttcagc ggcctctttt catacggttt
     6961 taaaatgccg gccatattat tggtgcttaa ccattttata aatcatgaaa ccggtcactc
     7021 cgaacagagc aagcaggaaa acgggcagga accaagagcc gagcccgtct gataagcccc
     7081 ttgccgcctg cgcagtctgc cactgtatgt aaagaaagat cgtaatcatg ataaatttta
     7141 tccattccag cgccagacgg cccaatttgt attgatactc tgcgttttct tcggtgattt
     7201 cgcccgggta gttataaatc caggggaatc tttcgaggat ggtaagggct gtgtagagta
     7261 tgactgccat gattggcagc ataagcagcg agccttttcc tccccatccg tccgcgcggc
     7321 cgtcggctcc gaaatgggtc ggaatactct cggggagatt gatcatcgag aaaacggtca
     7381 ggacgatcaa tccgagcacg ccaaagaggg ataaaatctg cagcagcttt tcaggctggc
     7441 tgtagggcag tttgattttc ggcctcggca tatgaagcgg tcctttccta tgttaagtac
     7501 agaaaccatt aagaatcaac agattaagta aatgatagca cattctgcca tggaggtaaa
     7561 tagaaaaacc tattgactta ccgaaataaa atgatataat attatctgta accaatacat
     7621 aattgaaata actacaaaac ggcaggtgtc gcgaatgcag aaacaaacgg gtgtcagtga
     7681 atttctctct atgcatcata tccgtccgtc ctatcagcgt gtcaagatca tgcaatatct
     7741 gcttgaccga aagagccacc caacgatcga agaaatttat agggcgctca ttccagatat
     7801 cccgacactg tcaaagacca ctgtttataa tacgctgaat ttgtttatcc gggaaaggat
     7861 cacccagccg ctttccattg aagaaaacca gacccgctat gacgcggata tttcggttca
     7921 cggccatttt agatgcttaa attgtggaaa aatttatgac ttctgcgttg acgcggaaaa
     7981 tcttgccgtc agcggcctgg atggctttca aatcgactcg cgttacattt tttatcaagg
     8041 catctgcccg gtttgccgga aagcggaagg ccgttcttct gcagagggga aaccggagca
     8101 tcaataaatt taaatgacga ggtgtgtaca aatgaaagat ttaaagggta ccaaaacaga
     8161 acagaatttg caggcggctt ttgcaggcga gtctcaggca agaaataaat atacttattt
     8221 tgcttccgcg gccaaaaagg aaggatttga gcagatcgcg ggaatcttcc aggaaacagc
     8281 ggataacgag aaggaacacg cgaaaatctg gtttaaatgt ctgaacggga taggcagcac
     8341 cgtggagaac ctcaaggcgg cagccgccgg agaaaactat gaatggacaa ccatgtataa
     8401 gaatttcgcc gcgcaggctc atgaggaggg ctttgccgag attgcggccc tttttgagat
     8461 ggttgccgat attgaaaaaa gccatgacga gcgttatcaa tcgctcctga aaaacattga
     8521 ggacggaaaa gtctatttaa aggatggatc ggtaatctgg aaatgccgca actgcgggca
     8581 tatccatgtc ggtaaaaacg caccggaaat ttgcccggtc tgcaaacatc ccaaagcttt
     8641 ctttgaagtg gccgccgata attattaagg ttttccgaag gctgaacaat ttacgattcc
     8701 ccataaacat aaaaatacga ccggcatata gccggtcgta tttttattgg aatcaactga
     8761 cacgtggggg gatactgtat tctcatgctt tgcgattcaa accggattga ttcccttcaa
     8821 agtgaaataa caggctgcaa agaaggatag accctaaaaa aattccaatt accgagacgc
     8881 tgatgaccgc attcagccgg gaagattgat accgcccatt atagagcgct tccaggcggt
     8941 ttgttttgta aaccgtgtca aaaaccaatt ctccgccggc ctttcggcca tttaaagcaa
     9001 agtggaccgc atgtttgttc ggggttccgg aaaaaagtaa aataccccct ttgctgattg
     9061 ccgtaacagg gcttaactta atcgagtaga gccttccctg ggagttttca atgtaagaga
     9121 tgggaaactc aacgtattga ttgtccacga tggaagaaga ctcgataatt tgactggaga
     9181 gaaccaggcc tttctcatca aacagctcaa atttaatccg gttcatgttt gataactctt
     9241 tcggctggac aagcagtttc acctttacag ccgaaaatcc gttttcttcg gcccgaaagc
     9301 tttgagtgac gttttcgact tttaatagat tcaccgccgt ggccccttgt gtgtcgggtc
     9361 ctttttttac cgtataacgg ggaagcttga tatacagaaa tgcggtgatc gcgcagccaa
     9421 gcagaatacc gaaaattaga gcaggaaagc gaatacgcct taaaatttta aaaagaaaat
     9481 tcataatatc atacctaccg ccttcacaat acaaaatatc gctggaacaa aataagataa
     9541 agagtataga aatccagcag tataaaggat gcggcgacaa ctttataaaa gagtcgaatc
     9601 cgtttttggg agaaaaattg ttcaaaaccc tttgtcatca ggattacaat gggaataatt
     9661 gcgggaaaca gatatctgcc ctgaggctga aagtcacagt tcagggaata atagacatgc
     9721 agcgcgaatg cgccgaaaat aacgaacaga aaggtaatag ccagcttgtt gctgaagagt
     9781 gatgcgattc cctctctttt tttctgtaaa aagcggtagg aaagaccgat aaatgaaata
     9841 agcataaggg caaaaattgc aaggtaataa tacggattta atgttatttc catatttcca
     9901 aaatcggccc aaaaggaaac gaacgaagtc gaaggccagt tagtaaaaac cagtagtccc
     9961 cccagcccat gccggattga cagcttggat atcagttcgg acggcttgta tcccatggcg
    10021 gcttttgttt cggaaatttt ggtggtagtc gataagccaa aaatatcccc gtgataataa
    10081 taggcgtttc gtataaaaaa ccagccgctg atcagcaata gaatgccgat gcacaggaga
    10141 agttttttaa aaaactccgg cgatctcttt tgagggtggg tgagaaggac aatcagcagc
    10201 gttgcgacga cgagaacgta gccgtttaaa taagttaaaa gcatcagacc ggatacgacg
    10261 cctgtcaaaa ccactgtttt aagatcccac tgcgtttcga ttcctctgat ccacagatag
    10321 acggtgacag cgctcagcat catcataaac gaatcctggt tcgtatagga atttgcgaaa
    10381 gaaaactgcg gcaggaacgc caaaaacacc gggagaaaat agcgggcaaa tgatttcctg
    10441 aatagtttag cccctatttt gtaagtgaaa aacacggtga ttacgccgga aagaaccgat
    10501 acctgacgtg aaatgatata agcgggcaga tgaatcccga ttgctttggc gaaaatgcaa
    10561 aaaaaggccg aaaccatata ggacaaaaaa ggcatcgcgg cataggttgc gccatagcct
    10621 ccgtataaca gtctcggatc ccccgccacc ggcagctgcc gatacttata gataaagtca
    10681 acaatatcaa agcgaaggac ttcgtcaggc gcctggccga atggggtaat caagctccat
    10741 gaaagatata gcacaaagga agcaagcaca agcagcagga caaaacgttt ttcgctgtcc
    10801 aacactgttt ttttcaatag catcacctac agaatgttat ttttaaacgg ccggccgcag
    10861 catgaaacgg ccgcctgaat ttgtatcatt agctgcaaaa gaacttcagg atctcttccg
    10921 tcacacgctc ggactgattt ctgcccagcc gatagtaaag cggcagccgc agcagcctct
    10981 cactttcttt ggtggtaaat tcatctttcc catgaaagcg ggcatatttt tttccggcag
    11041 gcgaactgtg gagcggtatg taatggaata cgctttcaat accgatcttt ttaaggtgtg
    11101 acataagagc cgttctcgtg ggtaagtctt tgcacttgat atagaaaagg tgggcgttgt
    11161 gggtgcattc cttaggaata taaggcagtt ccagaatgcc tctttcctcc agaggtttaa
    11221 gcgcttcata atagtaattc caggaattca tccggtcgga caaaatgtcg tccaccttat
    11281 cgagctccgc gtttagataa gccgcattaa tttcgctcgg caaatagctt gagccgatat
    11341 ccatccatgt gtacttatca acttccccgc ggaaaaaacg acttcgatcc gttccttttt
    11401 cgcggatgat ctcggctttc agtttataag cgtccgaatt gaaaacgatt gctccgccct
    11461 cacccatgga tatatttttt gtttcatgaa aactaaatgc cccaagactt ccgattgttc
    11521 cgaggcaccg tcccttatag gtcgagagga atgcctgggc cgcgtcttca attacaacaa
    11581 ggttgttttt tcgggcaatt tcacagattt catccatttc gcagctcaca ccggcataat
    11641 ggacaacgac aatcgctttc gttttaggtg tgattgcgtc ttcaatcagt ttctcgttga
    11701 tattcatcgt gtcagggcga atgtcgacaa aaacgggaat cgctccccgg agcaaaaaag
    11761 cgtttgccgt tgacacaaag gtataagaag gcataattac ttcgtccccg ggtttcaggc
    11821 cgagaagcag cgccgacatt tccagcgcgt gcgtaccgga tgtcgtcaga taagtattgc
    11881 gaacccccag ctgctttgaa ataatgtcgc tgcagctacg tgtaaacggc ccgtctccgc
    11941 tgatttttcg gttgactttt acagccttct caatataatc aagttcccct cccgcatagg
    12001 gcggtaaatt aaagggtatc aagttgattc ctccatccag ttctgatttg ctgaattatt
    12061 tcagataaaa aattaaaatg cctgccaaaa taatcagatt gccgagaagt tttcttcccg
    12121 tgattttttc ttttaaaaac agacggctca gtatcagaat aaaaacgtat cccatagact
    12181 ccaaaaccgg ggcgttttta aattcgagcc ctctaaaagc caacagggtg agaacggtcg
    12241 agagcgccag cagcccgtat ccgctgatca caagaacatt gcaatattcc tttaggaacg
    12301 actgatgtac cctgttgctg cttgttttca acattacctg tgagaccgaa gcgatcgcaa
    12361 cggaaaaaac catcaacaga acgtatttaa tcatgtttat acccacactt atcggagttg
    12421 actatggtta ttccgcaaaa caccacgata atccccacta aattttgaaa cgtcacggtt
    12481 tcgtgaaaaa gaagagcgga ccagatcatg gcccaaaaga tggctgtgcc acggttggca
    12541 taagcaatgg aaagatccac tttttttaaa acctgcagcc aaagcactgc gtaaattcct
    12601 aacagaaaaa tctcaagcgc ataaagcaga atgaatcctg cggatgtgaa cggatatgcg
    12661 gatgcaaact tagcggttac cgaagccaag gaatagagga tcataatcag ctgaacgaac
    12721 aatatattca aaagaaaact ttttttgtta ttctttccgt tcatcacagg aaacctcatg
    12781 cagctgcaaa agttcattca attgtttttt gctggaacag gttaaaatgt aatatcccaa
    12841 ccgtgtggaa ctgtccgtta ctttctgtaa gcccggcagt tcaatcgggc tgattcgata
    12901 gatggctgac ggacaaacct ttttgattct gttaagctga tataaatcat tttcatcaaa
    12961 aataaaccga ataaaagcga tcttcggctg attatatttt gtaaaatccg gactgtttcc
    13021 gcacgcgacg tcaattgcca tttttataaa atcatatccg gtggatagcc ggacgagatc
    13081 cgaaccaatg cagtctccgc ccatgcgcga cccgatttca ataatcgcaa tggaaccatc
    13141 gggcttgatt cgaaactcgg aatgcgacgc tccgaatttg atacccagcg cgtcaagccc
    13201 tttttgaatc gtcgtgacaa tctttgaaac tgtctgctca tccagagcga cgggctgcag
    13261 atgccccgtt tcaataaaat gcggcgaacc tgtggtgaat ttctgcgtca gggcaagcag
    13321 gcggtgttct ccctggtaag agatacattc cgcgctgtat tccgggccgt cgatgtattc
    13381 ttccacgatc gctttttttt caaaagacga atccagactg cattgaatgg cccgcgcaag
    13441 cccatccggc ccgagaactt tcgtgacccc gcggcttccg gaccggtcga ccggcttaac
    13501 aatcaaaggg tagtgaaatc cttttgtgtc aatcccgtct ttggaaccgg taagcgtaaa
    13561 gctcggcatt ggcaatccgt attttctgag ccggcggcgc atttcaaact tatttgttga
    13621 catcagggta cattccgcgc cgttgcccac aaggccaagc ttttcggcaa tataattgac
    13681 cgtaggaacc gcaaggtcgg aacccacaga taaaatgcct ttcggtgaaa tttttcggca
    13741 tatctctaaa atttcttctt tgttgatgat gctgatcgga taaaaatgat cggctgtttc
    13801 ggcgccgacc gcgccgtctt tccatgcgaa aacatgtgtt tcatagccaa gctcttttgc
    13861 tttgagaatc aacggatttt gaaaattatt cgctccgatg ataacgattg cgtgtttcat
    13921 agcgcctcca tagaacaaga atgttttaaa acacccataa cttcagtgtt taaaatcgag
    13981 gacatttact gctgatcttc cgacggcggc cgcttcatac tttcggtgtc atcctgtgtc
    14041 gggcggtcat ctttctgtgt caaatcccgg atcacaaatt gcggggtctt gttgacgctc
    14101 agatagattc ttcccacata ttccccaagc agcccgacgg aaaacagctg gattccgccg
    14161 ataaacagga caagggtaat tgttgaagtc cagccttcct gaatatttgc atgcgtgagt
    14221 ttgaaaataa taatgacaat cagcatgata aatgcgcaaa aggacataag cactcccatt
    14281 atggatgcta aacggagcgg cttaattgaa aaattagtaa aaccatttaa ccataaacct
    14341 aataattgct tgatgccgta accggaatgg ccgttttttc tggagttgtg gtggataggt
    14401 acacaggcaa tattgcctgt cgcctgcaaa aaaagaccgg gaagataggg gaaaggcttg
    14461 ttataattcc ggacaatatc aacgacatat ctgcgcgcaa taaagtaact tgacgttttt
    14521 atattactgc gtgtttcaag cacaaacgcc tgcataagct cgttcatttt tgtcccgaaa
    14581 ttccggaaag cgctgtgttg tttatgctca taaaaaccat aaactacatc aaagtttcct
    14641 tccgccattt tgttataaag tttcgggaat tcttcaggcg gagtttgcaa atcatcatca
    14701 agacatacga caaaatcgcc ggaagtttga ttcaatccgg ccatgagagc gcttgcctgt
    14761 ccgaagtttt ttgaaagttt aaacagtttt acctgtggaa cttttcttgt caattcgctg
    14821 caaacttcaa cagaatggtc tttgcttccg tcatccacaa gaataatttc aaaattcaat
    14881 ttgcaaggca taccgttaat ggttttgatg atccgtccta taacattttg aattgttttt
    14941 tcggaattgt ataccggtat cacaaacgat acttttgtca ctttatcctc tcctgctaat
    15001 gataattgta aaaaatttca tgccgtttat tattatacac acttatcaag aaaagaacag
    15061 gatttcaaaa gatatttaat cgcatagtgt ccgtattgta acaaattccc ttagatttaa
    15121 ccaaaaagta actttttttt ctgaaaagga ccttcaaaat gctgctttgt ttttgcttaa
    15181 aaagcaaaac agaatgctct caaaaaaaac ttgaaaagat tgaaaagaat tcgcggacag
    15241 ggaaaaagga agacccgtat aaacatttca tttatacggg tctgtgtatg gcgtttatca
    15301 tggaaaagca ggagtctgcc ggtttcgtgc cggcttcgcg cgggcaattc gattaggatc
    15361 cctcgtatgc cctgacgcgc agcggaattc ccatttgatc cgcaagacgc tgacaggcgg
    15421 caatttcctc aggggaaata atatcgacta cggagaaaac cacccgcgga acgatctgct
    15481 tgcaatcttc tgcaaattta agcattgcgt caaaggcgga ttctccgaaa cttggctggg
    15541 ttacccgcga atattccttt gcggtggccg cgttcaaact gatggatacg gtgtctgcca
    15601 gtcctttcag taagtgcgcg gtcggttttc cgttgatgag atccgaaagc ccattcgtat
    15661 tgatacgtat tttcatgtca ctgactccgc gcagatattt gcaggtttcc agcacgatat
    15721 ccagccgctc aagaggctct ccgtaaccac agaataccac ttcggggcag ccggttttat
    15781 cgtaattttc ataagccgca ataatttcct caagggaagg ttcacgttcc agccagagtg
    15841 agtctgcgtc gccaagagaa tccccgttct gccggatgca aaaaatacaa ttgcagggac
    15901 agcggttcgt aacattcaca taaagctttc cgccatattt ataaaaaata gtcatgaagc
    15961 tacctcccgt gtatcgtttg gttcattata aatcactttt catcgtataa caagagtacg
    16021 gtccgtcggg ctttctgtaa aatgatacaa tccggggcca tacctacccg ttcaattatg
    16081 agtattgacg atttgatttt acagggtttg ttttttgggg tgaaacgctt gtctaaagcg
    16141 cagtaaacca ttggttcgga agccgcaaaa cctgtgcatt tatccgaatt atatcaattt
    16201 taacggaagt gccgcgtttt gtaatcgaaa cgattatcga gtatactaag acagaatcaa
    16261 tgacaaagag gtgcgttatg aatcagagct tcgcatctgt ttcccgtgtt gcggccatca
    16321 atgatatttc cggatttggc cggtgttccc tcacagtcgc gatcccgata ctctctgcta
    16381 tggggattca atgctgtcct ctgccgaccg ctgtcttatc ccagcacacc ggatttgagc
    16441 acttttattt taacgatctg acgccgcaga tgtccgcgta tatcgaaagc tggaaactgc
    16501 aagggctccg ttttgatacg gtttacagcg gatttttggg ctcggaggag caaatccgtc
    16561 ttgttgacgg ctttatcacc gcgcagaaag aaaacgcctt gatttttgta gaccccgtca
    16621 tgggagacgg cggaaaggta taccccacct atacctccca aatgtgtgat ggaatgcgtg
    16681 aacttgtgca caaggcgaat atcattaccc caaacataac cgaggcatgc ctgcttaccg
    16741 gtacgcctta tccgggagaa gcgccgggca ttgccgccgc caaagaaatg gccgttaaac
    16801 tcgcggagct tgggcccgct tattctgtta tcacaggtat ttgcaggggc aatcaaatta
    16861 caagccttgc ttatgacggc aacgagaaaa acttttttac ctgttcggtg gagcgcaccg
    16921 acgtacctta tttaggaacc ggggatatct ttgcatccgt actctgcggc tgtatgacaa
    16981 aaggtcaaag tctgcggtca gcgctgcaaa atgcctgcga ttttgttcgt gatacaacgc
    17041 agtataccaa aaagcttggt tccccgatta tggacggcgt cgcgttcgag ccgctgcttt
    17101 ttcatttagg aggaaacatg gatgcataaa aacaacatag aaaagctcac gttcacagcg
    17161 gtatttatcg cattgatttt cgttacgacc gcttatatcc ttcatattcc cgtcgggaac
    17221 ggttatattc atctcggaga cagccttatt taccttgcgg caagtttcct tcctgcccct
    17281 tttgcgattg cagccgcagc attaggagca gggatgtcgg atgctctgac gggttttccg
    17341 cagtatgtgc tgttcacaat gctcattaag ccgctcaatg ccctttgctt ttctcaaaag
    17401 ggcgataagc ttctgactac gcgcaaccgt attgctcctt ttatcagcgc gcctattacg
    17461 gtagtcgggt attatctggc cgatgttatc cattacatcc aaactgattg ccgcttt
//
LOCUS       DATALY010000052         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_616028,
            whole genome shotgun sequence.
ACCESSION   DATALY010000052 DATALY010000000
VERSION     DATALY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616028"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..>2464
                     /locus_tag="VHR42_01595"
     CDS             299..>2464
                     /locus_tag="VHR42_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX3025910.1"
                     /translation="MKTVFQKAVSLILSLAVLLGTTPLVRMTASAAESGDVTAYYCDS
                     GLFKDSHYTETVDDAFRSTITGVQVGDDTQIIGDSAFESCKALTAIAIPGSVTTIGNR
                     AFYQCAKLKSVTFGKNSPLNLIGEYAFFQCTALTAIVIPDGVTAIGNEAFQLCGIKSV
                     TFGEGSKLKSIGESAFECCGDLTQIRIPDSVTTIGNHALQQCTNLKSVTFGEGSKLQS
                     IGEFAFLSDENLTDFEIPQSVTKIGREAFFFCSSLTRIEIPHGVTALGADFDDQTFGA
                     CTGLTCVTVSDSLPVHADTFNGCTSLSNLYVIGSQKPSAMREVTNVFFFSENPDGTYK
                     LEDYSGTGAGRVIPAELYGKDINPPIQEADLHEQEIIAYCDGANIYKNQDKTGAIGSG
                     DPYRFMISKVEFTGADIQTITAPGSYEGAFLNCTGLTEITVPASVTQIGSNSFRDCTG
                     LKSLAFENGSKLQSIGSQAFDGCVKLNGIDLPGGVTFIGPFAFNGCKSLTGIDLPGGV
                     TDIPSNTFNNCTGLTDIVIPDNVTKIEYMAFAGCTSLKNVTFGKDSKLQSIGIYAFRG
                     CTSLTGISIPKSVTEIASSAFSGCTGLTDITIPKSVTNFGYGVFSRCGSLNKIAVEPG
                     NTVLYTDDSGVLFYIDPTAGKTLVRCPVGKSGTYEIPNGIVRMDESSFFGCTGLTGVT
                     IPDSIQFISPYAFDGCTGLNFVKFGGSATINDWYSNYAFH"
BASE COUNT          641 a          599 c          603 g          621 t
ORIGIN      
        1 atgtcgatta cgctggtttt ctctaccccg cgctcttcaa acaacgccac tccggcttca
       61 aaaattttct cctttgtctt catcccttta cttttttcgg ccattttcga aatatcactc
      121 tttcatattg actttttgca atcaagatca tatactggat atatgacctc ggtcatattt
      181 atattatact acgaaacctt acatattgct acaaaaatct tgtcgtccgg caaaagagac
      241 ggtaggcgtc tgcttgtgtc aaattacagc aaaaagaaaa agcaggggga aatgcaaaat
      301 gaaaacggtt tttcaaaagg cggtatcact gattctctca ctggccgtat tgctcggcac
      361 aacgcctctg gtacgcatga ccgcgtccgc ggcggaaagc ggggatgtga ccgcctacta
      421 ctgcgactcc gggctgttta aggattccca ctatacagag acagtggatg atgcattccg
      481 aagcaccatt accggagtgc aggtagggga cgatacgcag ataataggcg attctgcctt
      541 tgaatcctgt aaggcgttaa cagccattgc aataccgggc agcgtaacaa ccattggcaa
      601 tcgggcgttt tatcagtgtg caaaattaaa aagcgtcact ttcggaaaaa acagtccgct
      661 gaatttgatt ggtgaatatg cttttttcca gtgtacggct ttaaccgcca ttgtgatacc
      721 ggacggcgta acggctatcg gtaatgaggc gtttcagctc tgtggcataa aaagcgtcac
      781 ctttggagaa ggcagcaagc tgaaatccat cggggaatcc gcgtttgaat gctgcggtga
      841 cctgacccaa atcagaatac cggacagcgt aacaaccatc ggcaatcatg cgctccagca
      901 atgcacaaac ctgaaaagcg tcacttttgg agaaggcagc aaactgcaat ccatcggcga
      961 atttgcgttt ttaagtgacg aaaatctgac cgactttgaa atcccccaaa gcgtaacgaa
     1021 gatcggcagg gaagcgtttt ttttctgctc ctccttgacc cggatcgaaa ttccacacgg
     1081 cgtaacagcc ctcggcgccg attttgatga tcagacgttt ggagcctgca caggcttgac
     1141 ctgcgttacg gtcagcgatt ctttgcccgt ccatgcggat acattcaacg gatgtacttc
     1201 cctgagcaat ctttatgtga tcgggagcca aaagccatcc gcaatgcggg aagtcactaa
     1261 tgtttttttc ttctcggaga acccggacgg gacttacaag cttgaggact attccggtac
     1321 gggagccggg cgggtgatcc ccgccgaatt atacggaaag gatatcaacc ctccgattca
     1381 ggaagccgat ttgcacgagc aggagatcat cgcttactgc gacggagcca atatctataa
     1441 aaatcaggat aagaccgggg caatcggctc aggcgacccg taccgcttta tgatttcaaa
     1501 ggtagaattc acaggtgcgg atattcaaac gattacagca cccgggtcgt atgaaggcgc
     1561 gttcctaaac tgcacgggtt tgacggaaat caccgtaccc gcaagtgtga ctcagattgg
     1621 cagcaactct ttccgcgact gcacgggcct gaaaagcctt gcgtttgaaa acggaagcaa
     1681 gctgcagtcc atcggttccc aggcatttga tggatgcgta aagctgaacg gaatcgacct
     1741 gcccggcggc gtaacgttca tcggcccgtt tgcgttcaac ggctgcaaaa gtctgactgg
     1801 cattgatctg cccggcggtg taacggacat tccgtcgaat acgttcaaca actgcacggg
     1861 tcttaccgat attgtgattc cggacaatgt gacaaaaatc gaatatatgg cctttgccgg
     1921 ctgtacgagc ctgaaaaacg ttacctttgg gaaagacagc aaattgcagt ccatcggcat
     1981 ctacgcgttt aggggctgta caagtttgac cggcatttcg attcccaaaa gtgtaacgga
     2041 aatcgcaagt tctgcgtttt ccggctgtac aggtttgacc gacattacaa tacccaaaag
     2101 cgtaacgaat ttcggctacg gtgtgttttc ccgctgcgga agcctgaata aaatcgccgt
     2161 ggagcccgga aatacggtgc tttacacgga tgacagcggc gttctgtttt atatagaccc
     2221 gacggccgga aaaactttgg ttcgctgccc agtcggaaaa tccggaacat acgagattcc
     2281 gaatggtatt gtaaggatgg atgagagttc gtttttcggc tgcacgggct tgaccggcgt
     2341 taccattccc gacagcatcc agtttatttc tccttacgca tttgatggct gcaccggttt
     2401 aaactttgtt aaatttggcg gatcagctac tataaatgac tggtattcga attatgcttt
     2461 tcat
//
LOCUS       DATALY010000053         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_98311,
            whole genome shotgun sequence.
ACCESSION   DATALY010000053 DATALY010000000
VERSION     DATALY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98311"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..792
                     /locus_tag="VHR42_01600"
     CDS             181..792
                     /locus_tag="VHR42_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017116305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025911.1"
                     /translation="MLVLAAFLTAFFSAFFLAVVFFTAVFAAAAFTVDFLAVVFTAVF
                     LAAVLFAAVFTVVILAVAAFAAFAVFAAVFVAVFFTVVTAVFLTATFFAAVFTAVFLA
                     VLLIAFFLAGVFLTAVFLTAVFFIGAFLTAACTAVFFTVAVFTADFAVSAFFTVLETG
                     ADAAGFALFVCAANAGTAQTETIIARLSTNDTIFFIDDYLFLF"
     gene            complement(1097..2989)
                     /locus_tag="VHR42_01605"
     CDS             complement(1097..2989)
                     /locus_tag="VHR42_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3025912.1"
                     /translation="MPGPRHNASGAKPKNVRKTLARISGYIFQQKFRFFTVLFFILIS
                     SSANVAGTYFIKPLLNQCIAPLVGKKVTPADFIPFIQMIAVMGCIYAIGVLSSLIYSQ
                     LMIKISNGTLNAVRRDLFNKMQDLPIKYFDTHTHGELMSRYTSDVDTLREAITMGVTQ
                     LLGSTITVVGTFIMMLVLSPILTLLIVVMLLIMFTIIKKVGGMSAKYFKKQQAAIGAA
                     NGYIEEMIEGQKVVKVFCHEDKVKKEFDTLNENLRQAATSANYSASVLMPTMGNLGYV
                     NYALTAVAGAVLVISGWMDIGSIASFLQYTRTFNQPITMISQQLNTVFAALAGAERIF
                     EVIDEQPESDDGYVTLVNAKKDAQGNLTETAERTGLWAWKHPHHDGAVTYTQLNGDVR
                     LHDVTFGYEPDKTVLHDISLYAEPGQKIAFVGSTGAGKTTITNLLNRFYEVPEGKIRY
                     DGINITKIKKDDLRRSLAMVLQDTHLFTGTVRENIRYGRLDATDEEIVAAAKLANADS
                     FIHHLPNGYDTMLTSDGTNLSQGQRQLLAIARAAVANPPVLILDEATSSIDTRTEALI
                     EKGMDKLMNGRTVFVIAHRLSTVRNSNAIMVLENGEIIERGSHEDLIKEKGKYYQLYT
                     GQFELS"
     gene            complement(2989..>3750)
                     /locus_tag="VHR42_01610"
     CDS             complement(2989..>3750)
                     /locus_tag="VHR42_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017473832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3025913.1"
                     /translation="DSEKVKDGSVVFEGVSFKYNPNAQANTLEQVSLNIKSGETVGII
                     GGTGSAKSSFVQLIPRLYDVTAGRILVGGRDVRSYKIEDLRDAVSMVLQKNVLFSGTI
                     KDNLKWGNDGASDEQIVAACKAAQAHDFIMSFPNGYDTDLGQGGVNVSGGQKQRLCIA
                     RALLKNPKILILDDSTSAVDTATDAKIRSALKEARRDVTTIIIAQRISSVCEADKIIV
                     LDDGRINAVGTHDELMETNEIYRDVYESQQKGVAV"
BASE COUNT          814 a          957 c          929 g         1050 t
ORIGIN      
        1 tccaaagaag aaggccgacg ggactggaat ttaattgtga tggagaaaga gttaatacaa
       61 cggtaaggat aaaataaaaa agacgttccc atttaaaatg gagcgtcttt tttatgtcca
      121 aatcggacgg gtattaaagg aagttaaaag ggctgattac ttcaggcagc tcccttggcg
      181 gtgctagttt tagctgcttt tttaacagct ttcttcagtg cgttcttctt ggccgttgtc
      241 ttcttcaccg cggtctttgc cgcagcggcc tttacagttg actttttagc cgttgttttt
      301 accgctgttt tcttggcggc cgtccttttc gctgctgttt ttactgtggt tatcttggct
      361 gttgctgcct ttgctgcctt tgctgttttt gcagctgtct ttgttgcggt ctttttcact
      421 gttgttaccg cggtcttctt gactgccact tttttcgctg cagttttcac cgctgttttt
      481 ttagccgttc tcttgattgc gttcttcttg gccggcgtct tcttaactgc tgtcttttta
      541 acagctgtct tttttatcgg tgccttcttg accgctgcct gtaccgcggt tttctttaca
      601 gtcgcagttt tcaccgccga ctttgcagta tccgcctttt tcacagtact ggaaaccggt
      661 gccgatgctg ccggctttgc attgtttgtc tgtgcggcaa atgccggtac ggcacaaacc
      721 gaaacgatca ttgcgagact gagcacaaac gacacgattt ttttcataga tgattacctc
      781 tttctatttt gatattcgga ttgcggaagg cacaaagatt tgcctaaccc tgattctctt
      841 actggaaaac atttccctcc tttcatgcac caaatattag tacgttcgtt tttaattatc
      901 aagtcacaat aagggcacga aacaaattta aggtaaattt aactatttat taaagattaa
      961 ttaagttgtt atattataat tgttcataag aaactgtata tttcttcata tatgaacgcc
     1021 tacagaaaag cgcacccaaa ggaatcaaaa agttttcctt cgggtgcgct tttaaatttg
     1081 ataataggaa ctacgttcag gacagctcga actgaccggt ataaagctga taatatttgc
     1141 ctttttcttt gatcaggtct tcgtggctgc cccgctcgat gatttcaccg ttttcaagca
     1201 ccatgatcgc gttggaattg cggacggtgg acagccggtg agcaatgaca aacaccgtgc
     1261 ggccgttcat cagcttatcc atgccctttt caataagagc ttccgtgcgg gtatcgatgg
     1321 aactggtcgc ttcatccaga atcagaaccg ggggattggc gaccgccgcg cgcgcgattg
     1381 cgagaagctg gcgctgcccc tggctcaggt tcgtgccgtc agaggtcagc atcgtgtcgt
     1441 agccgttcgg cagatgatgg atgaacgaat ccgcgttggc aagctttgcc gccgccacaa
     1501 tttcctcatc cgttgcatca agcctgccgt aacggatgtt ctcccttacc gtaccggtaa
     1561 acaggtgggt atcctgcaga accatggcaa gcgaacggcg aagatcatct ttcttgatct
     1621 tggtgatatt aatgccgtca tagcggattt tcccttccgg cacctcatag aaccggttca
     1681 ggaggttggt gatggtcgtt ttgcccgcac cggtcgaacc gacgaaagca atcttctgcc
     1741 ccggctccgc atagagcgaa atatcgtgga gcaccgtttt atccggctca tagccgaatg
     1801 taacgtcatg gagccttacg tcgccgttga gctgggtata ggttaccgcg ccgtcgtggt
     1861 gcgggtgctt ccacgcccag aggcccgtac gttcggcggt ttccgtcagg ttgccttgag
     1921 catccttttt ggcgttgacc agcgtgacat agccgtcgtc gctttccggc tgctcgtcga
     1981 tgacctcaaa aatacgctcc gcgccggcga gtgcggcaaa caccgtgttc agctgctggg
     2041 aaatcatagt gatcggctgg ttaaaggtcc ttgtatattg gaggaaagag gcgatggagc
     2101 cgatgtccat ccatccggaa ataaccagca ccgccccggc taccgcggta agcgcgtaat
     2161 tgacatagcc caggttgccc atggtgggca taaggacgct ggcgctgtag tttgcgcttg
     2221 ttgccgcctg cctcaggttt tcgttcagag tatcgaattc tttctttacc ttgtcttcat
     2281 ggcagaaaac ctttaccacc ttctgcccct cgatcatttc ttcaatataa ccgttggcag
     2341 caccgatggc agcctgctgc tttttgaaat actttgcgga catcccgccg accttcttga
     2401 taatggtaaa cataatcagc agcatgacga caatcagcag cgtcagaatc gggctgagca
     2461 caagcatcat aatgaaggtg cccactaccg tgattgttga tccgagtagc tgggtcacgc
     2521 ccatggtgat cgcctcgcgc agggtatcaa cgtcgctggt gtagcggctc atcagttcgc
     2581 cgtgggtatg ggtgtcaaag tatttgatgg gcagatcctg catcttgttg aacaggtcgc
     2641 ggcgtaccgc gttgagcgtg ccgttcgata ttttgatcat aagctggctg taaatcaggg
     2701 aagatagaac gccgatcgcg taaatgcatc ccatgaccgc gatcatctga ataaacggga
     2761 tgaaatccgc aggggtgacc tttttgccga ccagcggcgc aatgcactgg ttcagcaggg
     2821 gctttatgaa atacgtaccc gcaacattgg cggaagaact gatcagaata aagaacagga
     2881 cggtaaaaaa ccggaatttc tgctggaaaa tgtacccgga aatccgggca agggtcttgc
     2941 ggacattctt gggctttgct ccggatgcat tgtgtcttgg tcccggcatc agacagccac
     3001 ccctttctgc tgcgattcat agacatcgcg gtaaatctca ttggtttcca ttaactcgtc
     3061 gtgtgttccg accgcattaa ttctgccgtc gtccagcaca atgattttat ccgcttcaca
     3121 taccgaagag atacgctgcg caataatgat cgtcgtaacg tccctgcgcg cctctttcag
     3181 cgcggaacgg atttttgcgt ccgtcgcggt atccacagcg ctggtgctgt catccagaat
     3241 caggattttc ggattcttga gcagcgcgcg cgcaatgcag aggcgctgct tctggccgcc
     3301 ggacacattg acgcccccct gcccaaggtc ggtatcatag ccattgggaa agctcatgat
     3361 gaaatcatgg gcctgtgcgg ctttgcaggc tgccacaatc tgttcgtcgc tcgcgccatc
     3421 attgccccac ttaaggttat cctttatcgt accggaaaac aaaacgtttt tctgcagaac
     3481 catgctgacc gcgtcgcgca aatcctcgat tttatagctg cgcacatcgc gcccgccaac
     3541 cagaatccgc cctgcggtca catcgtaaag acgcgggatc agctggacaa agctgctctt
     3601 tgcagagccg gttcctccga tgatgccgac ggtttcgccg gatttgatat tcaggctgac
     3661 ctgctcaagc gtattggctt gcgcattcgg gttatattta aaggatacgc cctcaaatac
     3721 aacggaaccg tccttcactt tttcgctgtc
//
LOCUS       DATALY010000054         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_98564,
            whole genome shotgun sequence.
ACCESSION   DATALY010000054 DATALY010000000
VERSION     DATALY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98564"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VHR42_01615"
     CDS             <1..499
                     /locus_tag="VHR42_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3025914.1"
                     /translation="DSIGLEYNGKAVGAGCEDAESGPLTLNLGIVRCDGAKAESVIDI
                     RYPVTKNGGEIAGPILEHAKTHGIEAAVKSDSKPLMVDPDSELIEKLRSAYKTATKEE
                     PRLYSTGGGSYARSLENRGVAFGAGVRPCSFYNIHGADEFLDIEDFMKHCQICLQAMY
                     ELGCC"
     gene            743..1612
                     /locus_tag="VHR42_01620"
     CDS             743..1612
                     /locus_tag="VHR42_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HEX3025915.1"
                     /translation="MPTRAEIRDRMVKAGVKICGMVDFCCLRDKLIPCSALRRLPVNA
                     KTVIMALFPYYLPLEGDRRISRYAVPRDYHLTVGESLTAASKKLKEDFPDNEFVPFCD
                     ASPIPEVYAARLAGLGAAGRHGLLINREFGSFVFIGEIVTDLEVSGEMEKAGEDCLSC
                     GLCVRSCPGGAIGANGIDRERCVSHLTQKKGVLTGEQERLIQKSGSLWGCDICQDVCP
                     MNRKIRETGLKPFRDDLKMTITPDELEQEDFAVRMKDRAFLWRGIGVLKRNLRILKGA
                     PADNKDKSSVKEC"
     gene            1614..2024
                     /locus_tag="VHR42_01625"
     CDS             1614..2024
                     /locus_tag="VHR42_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3025916.1"
                     /translation="MQVKEVMTTRVVSVAPNSNVKDAAALMNKHNIGSVPVVDSGMVR
                     GIITDRDIVLRCVAEGRDANNLRVSDICSSGAIAVKPVQSLSDAIHVMASEQVRRLPV
                     VDDGKLVGMLSLADIAREKSGMELAGAISDISMP"
     gene            complement(2176..2826)
                     /locus_tag="VHR42_01630"
     CDS             complement(2176..2826)
                     /locus_tag="VHR42_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX3025917.1"
                     /translation="MFSPCGRKTEKVYCKSCEFLVDSQIESLPVNPFDIIRANRWGLV
                     TYQELADLLGTSQEEIIQASASCDGFTIFNGRNYCIAYNSRVRQYSRIAFTLLHEAAH
                     IVLGHFAECDSFHNNLAFYRQFESEANFFASNVMAPYVVMRECGLLTPQLLHCACGLS
                     YEAAERRLAGYRCWSPRAIDKKVFSNFSAFIRFSRIQSVHRPDIEIIRDETVSAAK"
     gene            complement(2826..3179)
                     /locus_tag="VHR42_01635"
     CDS             complement(2826..3179)
                     /locus_tag="VHR42_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3025918.1"
                     /translation="MSIGNRIRERRESLGLTLEEIGTRLGVNRSTVKRYESGETKRIP
                     ISTIKKLASILNTTPEYLLGLDQSKEGSGNKEEFDPDVIILARLVQNIPKEKREMLGR
                     IIHTMSEIADEEMKK"
BASE COUNT          802 a          764 c          880 g          790 t
ORIGIN      
        1 tgacagtatt ggcttggaat ataacggaaa ggctgtgggc gcgggctgtg aagacgctga
       61 atccggccct ctgacgctga atcttggtat tgtgcgctgt gacggggcaa aggccgaatc
      121 ggtgattgac atccggtatc ccgttaccaa aaacggcggg gagatagctg gcccgattct
      181 tgaacacgcc aaaacccacg gaattgaagc cgcggtaaaa tcagattcga agccgctgat
      241 ggttgaccct gacagcgaac tgatcgagaa gctgcgcagc gcctacaaga cggccacgaa
      301 ggaagagccc cgtctttatt ccaccggcgg cgggagctat gccaggtcgc ttgagaaccg
      361 gggcgtcgcg tttggcgcgg gagtccggcc ttgctcgttt tataatattc acggcgcgga
      421 tgaatttctg gacattgaag attttatgaa gcactgccag atttgccttc aggcaatgta
      481 tgagcttggc tgctgttaac aaattaaaaa aaacaagaca aaggcccggg cgggggagcc
      541 cgagcctttg ccatatagcc tgaggggtaa tttgaggagg gtagagcatg taccggatga
      601 tgggtgcggc ttccatctga tcggtacagt cttattataa ggcctcgtcc ctgatgattt
      661 gttacatatc tatgaacaaa gcgttacgag agaatcaatt cgagggcagg caaagcaagt
      721 ttatagatag ggtgtgaaca gaatgccgac aagagctgag ataagagacc gaatggtaaa
      781 agcgggcgtg aaaatttgcg gtatggttga cttttgctgt ctcagggaca agctgatccc
      841 ctgctcggcg ctcaggcgcc tgccggtgaa tgcgaagacg gtcattatgg ctctgtttcc
      901 ctattatctg ccgctggaag gagaccgccg gatttcccga tacgcggttc cgcgtgatta
      961 tcatctgacg gtgggggaat ctctgacagc ggcgtcgaag aaactcaaag aggactttcc
     1021 ggacaacgag tttgttccat tctgcgacgc ttccccgatt cctgaagttt acgcggcgcg
     1081 gcttgccggg cttggcgcag cgggcagaca cggccttctg atcaaccggg aattcggctc
     1141 tttcgtcttt atcggcgaaa ttgtaaccga tcttgaagtc agcggcgaga tggagaaagc
     1201 cggggaggac tgtctttcgt gcgggctttg cgtgagatcc tgcccgggcg gggcaattgg
     1261 cgcgaacgga atcgaccggg aacggtgcgt gtcgcattta acgcagaaaa aaggcgttct
     1321 taccggggaa caggagcgcc tgattcaaaa atcagggagc ctctggggct gtgacatttg
     1381 tcaggatgtc tgccccatga atcggaaaat ccgtgaaacc gggctcaaac cgtttcggga
     1441 tgatttaaaa atgactatta cccctgatga actggaacaa gaggattttg ccgtaagaat
     1501 gaaagatcgt gccttcctct ggagaggaat cggtgtttta aaacggaacc tccgtatatt
     1561 gaaaggcgct cctgcggata ataaagataa atcatccgtg aaggagtgtt aaaatgcagg
     1621 taaaagaagt tatgaccacc cgcgttgtca gtgtggctcc gaattccaat gtaaaagacg
     1681 cagccgccct gatgaataag cacaacatcg gctcggttcc ggttgttgac agcggcatgg
     1741 tgcgcggaat tattaccgac cgcgacattg tgctcagatg tgttgcggaa ggccgcgatg
     1801 ccaataacct gagagtatcg gatatctgct ccagcggcgc gattgcggtc aaaccggttc
     1861 aatccctttc cgacgccatt catgttatgg catcggaaca ggtcagaagg ctccccgttg
     1921 tagatgacgg aaaacttgtg ggtatgctta gtcttgcgga cattgccaga gaaaaatcgg
     1981 gtatggagct cgcgggagca atttcggaca tatccatgcc ctaatgcata aaaaagcagg
     2041 gcgccggtgt tcagatcatc acgcccggct taaaacagcc gttaaaaaaa gaagacggcg
     2101 gctgccgtct caacttcttt taacagataa cagaataaaa atgaatcatt tataaaagcc
     2161 ggctctgccg gctttttatt ttgccgccga aacggtttca tcacggataa tttcaatatc
     2221 cggccggtgc acgctttgaa ttctgctgaa tcggataaaa gcggagaaat ttgaaaacac
     2281 ttttttgtca attgcacggg gcgaccagca ccgatagccg gccagccttc tttccgcggc
     2341 ctcataggaa agcccgcagg cgcagtgaag cagctgcggg gtcagcaaac cgcattctct
     2401 cattactaca tagggagcca tgacattgga agcaaaaaaa tttgcttccg attcaaattg
     2461 ccggtagaag gccagattgt tgtgaaaaga gtcgcattcc gcaaaatggc cgagaacgat
     2521 gtgcgcggct tcatgcagca gcgtgaaggc aattctgctg tattgcctga ccctgctgtt
     2581 gtaggcgatg cagtaattcc ggccgttaaa gatcgtgaag ccgtcgcagc tcgccgaggc
     2641 ctgaataatt tcttcctgac ttgtgcccag taaatccgcc agctcctgat aggttacaag
     2701 cccccatctg tttgccctga taatgtcgaa agggtttacg ggcagggatt ctatttgaga
     2761 atcaaccaga aattcacagg atttgcaata gactttttct gtcttgcggc cgcagggaga
     2821 aaacattatt ttttcatctc ctcgtcggcg atttccgaca tggtatgaat gatcctgccc
     2881 agcatctccc ttttttcctt gggaatattc tgaaccagcc gggcgagaat gatcacatcc
     2941 ggatcaaatt cctctttgtt tccggaacct tcttttgact gatccagacc gagaaggtat
     3001 tccggggttg tgtttaaaat agaagcaagt ttttttatag tcgagatggg aatccttttg
     3061 gtttccccgc tttcatagcg tttcaccgtc gaacgattta cgccaagccg ggttccgatt
     3121 tcctcaagcg tcagcccgag tgattccctg cgctccctga ttctgttgcc tatgctcata
     3181 acggcaggcg gctcctttca gatatctttt gtcataccgc ttctgataac tttatt
//
LOCUS       DATALY010000055         6076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_789494,
            whole genome shotgun sequence.
ACCESSION   DATALY010000055 DATALY010000000
VERSION     DATALY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789494"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..881
                     /locus_tag="VHR42_01640"
     CDS             18..881
                     /locus_tag="VHR42_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX3025919.1"
                     /translation="MILTLLSVAAGLRFLGFVLIAAGGFQSVTSTRFNFAYFDRGKRL
                     DSAVTEKETVSPSKCGQIELDLVNADLTVSSTDGSDITLTYQRFYEKEWSYSLQGRTM
                     ELKYGQKHGWLSSLTNWVDNSGWGDSSRRTVVLSIPRNAKAAYKIINVNGSVHISGIS
                     ADSLNMNGVNTQAEISDVVSAGDIRLNSVNGGITINRAKMPALRFSGMVNADCTVNQS
                     NADIKAGSFVNSTLNVTTANTDDYYIVYKTVGGVVNVQDGHYSGKGSIGAKDRAHRID
                     FEGVNSNLSVK"
     gene            1063..2700
                     /locus_tag="VHR42_01645"
     CDS             1063..2700
                     /locus_tag="VHR42_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3025920.1"
                     /translation="MKTKRIISFFFLFLCIFVGIILRLFWLSQSDNEYKQAAASHGTY
                     NLTLSSNRGTIYDTNLKPLVNNTDVYYAAVSPTTKNSSSQLEALRSHVLNFKTFLTRM
                     EESLPFLTRVDSLNIQAKGVTMIKTTQRYSDSALAPHIIGYLGSDGNGVSGIEKSYNS
                     QLAKYSQTISAKFAIDANGRSLEGVPADIEVDGDSTAGGVVLTIDRNIQQIAQKAADK
                     YLKTGAIVVMDVKNGDILASVSNPGFSQNSLGAALTNQESPMLNRAFSAYNVGSIFKI
                     AVAASALESGVSSGYSINCSGSVDIYGRTFNDEKKGGHGSQNMSQAMANSCNVYYISL
                     GQKIGGNKILDMAKKLGFGTSGELAPGLKPDDGVLPTSSSLEIPAALANFSMGQGNFM
                     ATPVQVARMISAVANGGLLPTPRLVKELVDDKMSVVKDNPSAVPDRVMSAEIAKTLKN
                     FLIGTVKSGTGVPAAPTYGGAGGKTATAETGWVKNGKAINQAWFAGFYPADNPKYAIV
                     ALLENGVAGGADAGPAFKYIADSLAPSLGYPTVSESK"
     gene            2893..5523
                     /gene="alaS"
                     /locus_tag="VHR42_01650"
     CDS             2893..5523
                     /gene="alaS"
                     /locus_tag="VHR42_01650"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX3025921.1"
                     /translation="MEWTGLNELREKFLSYFESKGHIRLPSFPLVPQDDNTLLLINAG
                     MAPMKKYFLGQQTPPGKRVVTCQKCIRTPDIERVGKTARHGTFFEMLGNFSFGDYFKS
                     EATAWGWEFITKVLELPEDRLWVSIYESDDEAFEIWTKEVGVKPERIVRFGKEDNFWE
                     HGTLPCGPCSEIYFDRGEKYGCGRPECAVGCDCDRYVEFWNIVFQQFDSDGKGHYTPL
                     KKPNIDTGMGLERLACVLQDVNNLFEVDTIANIMKHVIRIAGVSYGANPKQDVSLRVI
                     TDHIRGTVFMVADGVIPSNEGRGYVLRRLIRRAARHGRLLGISGAFLTDICDTVIREN
                     SSAYPELNEKKDYIKRVISMEEERFSTTIDQGLQLLNAMIEKMERNQQTVLSGADAFR
                     LYDTFGFPLDLTSEILSERSMRADEDGFQSLMKEQRERARAARNAGGDGPVEEIDDDE
                     IPDTVFVGYKTLECDAKIAGIFAHGEKAGFAGEGEKISVILDQTPFYAESGGQVGDSG
                     VLKTENAVVRITGCKKTPSGRHIHSGVVLTGRITIGDSVKANVDESRRSAIARDHSVA
                     HLLQASLRKVLGSHVQQAGQLVDEHRVRFDFTHFSALTGEELVRVEGMVNEAILSALT
                     VSVTEMPIAEAKKTGALALFGEKYGSVVRVVDMGSVSTEFCGGTHVKNTAQIGLFKIV
                     SESSVAAGVRRIEGATGLNLLDLLNNDGKLINETAAVLKSTPGELSRKAGQLVAELRV
                     KEREIDSLNRKMAEMQLDGLMKDAKEIKGFKVISASFPGAAPDALRLITDRIREMEPN
                     SVSVLAGVQEGKINFVAACGKAAVDKGAHAGKIIKQVAAAAGGGGGGRPDSATAGGKD
                     VSRVGDALNLVSKLVEELAG"
     gene            5570..5908
                     /locus_tag="VHR42_01655"
     CDS             5570..5908
                     /locus_tag="VHR42_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX3025922.1"
                     /translation="MDCLFCKIAAGEIPSNTIYEDETVRAFYDIDPKAPVHFLVIPKQ
                     HISSPAEINSENSAVVAHVYEVIAMLSEKLKLEKGFRVVANCGEEGGQTVGHLHFHVL
                     AGRLLAWPPG"
BASE COUNT         1487 a         1484 c         1720 g         1385 t
ORIGIN      
        1 ccgcatgatg aaaaaaagtg attcttacac ttttatcggt agccgcggga ttgaggtttc
       61 ttggatttgt cctgattgct gcgggcggct tccaatccgt tacatctact cgttttaact
      121 ttgcttattt cgaccggggg aagcgccttg attccgcggt tacggaaaag gagacggtca
      181 gcccgtcgaa gtgcgggcag atcgagcttg atctggtgaa cgccgatctc accgtttcga
      241 gtacggacgg gtcggatatt actctcacct atcagcgttt ctatgaaaag gaatggtctt
      301 acagtctcca ggggcggacg atggaattaa aatacggcca aaaacacgga tggctcagca
      361 gcctgacaaa ctgggtcgac aacagcggct ggggcgacag ctcccgtcga acggtggttc
      421 tgagcattcc tcgaaacgcc aaagcggctt acaaaatcat aaatgtaaac ggcagcgtac
      481 atatcagcgg catctcggct gattcattaa acatgaacgg tgtcaatacg caggcggaaa
      541 tatccgatgt ggtttcagcg ggagacatcc ggcttaacag cgtcaacggc ggaatcacga
      601 ttaaccgggc gaagatgccg gcgctgcggt tttcaggcat ggtcaacgcc gattgcacgg
      661 tgaatcaatc gaatgccgac ataaaggcgg gtagtttcgt caacagcact ctgaatgtca
      721 cgacagccaa tacggatgat tactatatcg tttacaaaac ggtcggcggc gtagttaacg
      781 ttcaggacgg tcattatagc ggcaagggaa gcataggcgc taaagacaga gcccatcgaa
      841 tcgactttga gggcgtcaac agcaatttgt ccgtaaaata aaaacgtcgg ctttcccgat
      901 catcccaagc atggaccttt ccgacacttt gagtcgggcc gtttcggccc ggctttcgtt
      961 ttgtaaggaa aaactgtatg taatttttca tcctggttca taataccaat agaggccgca
     1021 ttttgaaatc aattatgaat gaaatcagga aggggacaca ggatgaaaac caaacgtatc
     1081 atttcttttt tctttctttt tctctgcata tttgtgggga taatcctgcg gcttttctgg
     1141 ctgtcccagt ccgataacga atataaacag gcggccgcgt cgcacggaac ttacaacttg
     1201 acactgagca gcaaccgggg aacgatttac gatacgaatt taaaaccgct ggtgaacaat
     1261 accgacgtct attacgcggc ggtatcccca acgaccaaaa attcttcaag tcagctggag
     1321 gcgctccgct cacatgtatt gaattttaag actttcctga ccagaatgga agaaagcctt
     1381 ccgtttttga cccgtgtcga ttcgctgaat atacaggcga aaggcgtgac catgatcaaa
     1441 acaacccagc gttacagcga cagcgccctc gcgccccata taatcggcta tcttggcagc
     1501 gacggcaacg gcgtgtcggg aatcgaaaaa tcctataaca gtcagctggc aaaatattcc
     1561 cagacaatca gtgcaaaatt cgcgatcgac gctaacggca gatcgctcga aggggttccg
     1621 gccgatattg aggtggacgg agacagcacg gcgggaggcg tggtgctgac catcgaccgg
     1681 aatatccagc agatcgccca gaaggcggcg gacaaatatc tgaagaccgg cgcgattgtt
     1741 gtcatggacg tcaaaaacgg tgatatcctc gccagtgtaa gcaacccggg attttcacag
     1801 aacagtctcg gagccgcgct gacaaaccag gaatccccga tgcttaaccg ggcgttttcc
     1861 gcttataacg tggggtcgat ttttaaaatt gccgtagcgg cgtccgcgct cgagtcgggg
     1921 gtgtcgtccg gttattccat caattgctcc ggcagcgtgg atatctacgg ccgtaccttc
     1981 aatgatgaaa agaaaggcgg acacggctct cagaatatgt ctcaggctat ggccaattcc
     2041 tgcaatgtct attacatttc cctcgggcag aaaatcggcg gaaacaagat ccttgacatg
     2101 gcgaaaaagc tggggttcgg aacctccggc gagcttgctc cgggcctgaa acccgatgac
     2161 ggggttcttc ccacatcaag cagtctggaa attccggctg ctcttgccaa tttctccatg
     2221 gggcagggga actttatggc cactcccgtt caggtggcca gaatgatcag cgcggtggcg
     2281 aacggcggcc tgcttccgac cccgcgtctt gtcaaggaac tggtggatga taaaatgagc
     2341 gttgtgaaag acaatccatc cgctgttccg gatcgggtga tgagcgctga gatcgcgaaa
     2401 accctgaaaa atttcctgat cgggacggtt aagtccggaa ccggcgttcc ggcggcccca
     2461 acctacggcg gagccggagg aaagacggcc acggccgaaa cgggatgggt caagaacggt
     2521 aaggcgatta accaggcgtg gttcgcagga ttttatccgg cggataaccc gaaatatgcg
     2581 attgtcgccc tgcttgaaaa cggcgtggcg ggcggagccg acgccgggcc ggcttttaaa
     2641 tatattgccg acagtcttgc gccgtcgctc gggtacccga ccgtgtcgga aagtaagtaa
     2701 acgccgcgcg gtgaagcgga tcggtgaaat cttttgccct gtttttcctg ttttcacttg
     2761 aatttgttaa ttttgctgtg atataatctt aatatgtgct attatgccga tgatgacgct
     2821 ccgttcctgg gcaaacagcc cgcggaacgg attttcattg attgaatata ttcagggggg
     2881 actccgaacg gtatggaatg gacaggctta aacgagttga gggaaaaatt tctttcctat
     2941 tttgaaagca aaggccatat ccggctgccg agctttccgc tggttccgca ggatgacaat
     3001 acgcttctgc tgattaacgc gggcatggcg ccgatgaaaa aatattttct cggtcagcag
     3061 actcctccgg gcaaacgggt ggtcacatgc cagaaatgta tccgtactcc ggatatcgag
     3121 agggtcggaa aaactgcccg ccacggtact ttttttgaaa tgctcggtaa tttttcgttt
     3181 ggcgactatt ttaaaagtga agctacagct tggggctggg aatttatcac gaaggttctg
     3241 gagctgccgg aagacaggct ctgggtttcc atctatgaga gcgacgacga agcgtttgaa
     3301 atctggacaa aagaagtcgg cgtcaaaccc gaaagaattg tccgttttgg caaagaagat
     3361 aatttctggg agcatggcac attgccgtgc ggaccctgct cggagattta tttcgaccgc
     3421 ggcgagaaat acggctgcgg cagaccggag tgtgcggttg ggtgtgactg cgaccgttat
     3481 gtggaatttt ggaatatcgt gttccagcag tttgacagcg acggtaaggg acattatacc
     3541 ccgctcaaaa agcctaatat tgacacagga atgggtctgg agcgtcttgc ctgcgtcttg
     3601 caggacgtga ataatctttt tgaagtggac accattgcca atatcatgaa acatgtcatc
     3661 cgtattgccg gagtttccta cggtgccaac ccgaaacagg atgtctccct acgcgtgatc
     3721 accgatcata tccggggcac cgtgtttatg gtggccgacg gtgtgattcc gtccaatgaa
     3781 ggccggggct acgtgctcag acgtctgatt cgccgggcgg cccgtcacgg ccggctcctt
     3841 ggaatcagcg gggcgttcct gacagatatc tgcgacacgg tgattcggga aaacagctcg
     3901 gcctatcctg agctgaatga gaagaaggat tacatcaagc gcgttatttc aatggaagaa
     3961 gaacgcttct ccacgacaat tgatcagggt ctccagctcc ttaacgcgat gattgaaaaa
     4021 atggagcgga atcaacagac cgttctttcc ggcgcggatg cgttccgcct ttatgacacc
     4081 ttcgggtttc ctcttgatct gacctccgaa attttgtccg aacgctcgat gagggcggac
     4141 gaagacggtt tccagtctct gatgaaggaa cagcgcgagc gggccagagc ggccagaaac
     4201 gcgggcggag acggcccggt tgaagaaatt gacgacgatg aaattcccga tacggttttt
     4261 gtcggataca aaacgctgga atgcgacgcg aaaattgccg gaatattcgc tcatggggaa
     4321 aaagccggtt ttgccggtga aggcgaaaag atttcagtca ttttggatca gacgcccttt
     4381 tatgccgaaa gcggcggtca ggtgggggat tccggagtgc tgaaaaccga aaacgcggtc
     4441 gttcgtatta cggggtgcaa aaagacgcct tcgggcagac atattcactc cggagttgtc
     4501 ttgaccggtc gaattacgat cggcgacagc gtgaaggcaa atgtggatga aagccgccgc
     4561 agcgccattg cgcgcgacca cagcgttgct catctgcttc aggcaagtct gcgcaaggtg
     4621 cttggcagcc atgttcagca ggccggccag ctggtggacg agcatcgcgt ccgttttgat
     4681 ttcacccatt tctcggcgct caccggcgag gagctggtac gggtcgaggg catggttaac
     4741 gaggcgatcc tctcggcgct gacggtcagc gtaacggaaa tgccgatagc cgaagcgaaa
     4801 aaaaccgggg ctctcgcgct tttcggcgaa aaatacggaa gtgttgtccg ggttgtcgat
     4861 atgggttcgg tctcgaccga attctgcggc ggaacccatg tgaaaaatac cgcccagatc
     4921 gggcttttca aaatagtcag cgagtcatcc gttgccgccg gtgtgagaag aatcgaaggc
     4981 gcaaccggcc tgaatcttct ggacctgctc aataacgacg gaaaactgat caatgaaacg
     5041 gctgctgttt taaaatccac tcccggtgag ctttccagaa aagccgggca gcttgtcgcc
     5101 gagcttaggg tgaaggaacg cgaaatcgac tcgcttaacc gcaaaatggc cgaaatgcag
     5161 ctcgatgggc tgatgaaaga cgcaaaagag atcaagggat ttaaagttat cagcgcttct
     5221 tttccgggag ctgctcccga tgcgctccgg ctgatcaccg accgtatccg cgagatggag
     5281 cccaattccg tgtccgtgct tgcgggcgtt caggagggta agatcaattt cgtcgccgcc
     5341 tgcggaaaag cggcggttga caagggcgcc cacgccggaa aaatcatcaa acaggttgcg
     5401 gccgcagcgg gcggcggagg cggcggacgc cccgacagcg caacggctgg aggaaaagat
     5461 gtttccaggg tcggcgacgc tctgaatctg gtttcaaagc tggtcgaaga gcttgccgga
     5521 taatttccgg cgggcagtcg ataaatgaat gagaaggagg cttgtgcgga tggattgcct
     5581 gttttgtaag atcgcggcag gggagattcc ttccaatacc atttatgaag acgagaccgt
     5641 ccgggctttt tatgatattg acccgaaggc ccccgtccat ttccttgtga ttcccaaaca
     5701 gcatatttcg tctccggcgg agattaactc cgaaaacagc gctgttgtcg cgcatgtcta
     5761 tgaagtgatc gcaatgcttt ccgaaaaact gaagcttgaa aagggctttc gcgtcgtagc
     5821 caattgcggc gaagagggcg gccagacggt agggcatttg cattttcatg ttcttgcggg
     5881 ccggctgctg gcgtggcctc cgggctgatg cgcggcggct gtccgccgtc tcgcggggaa
     5941 cgaaaaaatt acgcgcaatg cgtttggagg aactcacatg gatacatata cgctgataac
     6001 tttaaatccc ttgatctctt ttgcgtcttt catcagccca tcgagctgca tttcggccat
     6061 tttgcggtta agcgag
//
LOCUS       DATALY010000056         7561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_185330,
            whole genome shotgun sequence.
ACCESSION   DATALY010000056 DATALY010000000
VERSION     DATALY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185330"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /gene="trxA"
                     /locus_tag="VHR42_01660"
     CDS             <1..286
                     /gene="trxA"
                     /locus_tag="VHR42_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX3025923.1"
                     /translation="FEEEVMQSKLPVLIDFWAPWCGPCKMVSPIVDEIATEGEGSLVV
                     GKINIDEEPDLATRFNVFSIPTLIVVKDGEVQATSVGARAKEDIKQTLGI"
     gene            487..1245
                     /gene="ispD"
                     /locus_tag="VHR42_01665"
     CDS             487..1245
                     /gene="ispD"
                     /locus_tag="VHR42_01665"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX3025924.1"
                     /translation="MGLIQTVRNRMDYWKDRGEVPPVTAIVVAAGKGERMGGEASKQL
                     LSVCGMPVIARTLGAFENCRSVNEVVVVARDEDMVEISRIVKDFELTKVVRIVRGSDT
                     RQRSALCGLEAAGEAAYFAIHDGARPLVSPECIDRVVEGALRHGAAAAAVRVKDTVKI
                     ADDEGFVLSTPDRSRLWSIQTPQVFEAGLYRGAVKKAVEEQADYTDDCQMIESVGQKI
                     RLIEGEYTNLKITTPDDILAAEAYLGEGEESETF"
     gene            1242..1730
                     /gene="ispF"
                     /locus_tag="VHR42_01670"
     CDS             1242..1730
                     /gene="ispF"
                     /locus_tag="VHR42_01670"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX3025925.1"
                     /translation="MRIGHGYDAHRFEMGRKLILGGVEIEYEMGLLGHSDADVLTHAV
                     MDAILGAAALGDIGRHFPDTDEKYKGADSLVLLREVMAMAEKKGYRLGNLDATVIAQA
                     PKIKPYLDRIRRNLAECCRVDPDSVNVKATTEEGMGFTGRLEGVSAHAVCLLCKLPEC
                     DR"
     gene            2024..2248
                     /locus_tag="VHR42_01675"
     CDS             2024..2248
                     /locus_tag="VHR42_01675"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Se/S carrier-like protein"
                     /protein_id="HEX3025926.1"
                     /translation="MSRQFIVVSSITYAYKGRDALQNKGFSAYIERAPKELSSCGCHY
                     CVYFKNCSVEYATEILKSAHVRIISTGVTQ"
     gene            2288..3436
                     /locus_tag="VHR42_01680"
     CDS             2288..3436
                     /locus_tag="VHR42_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3025927.1"
                     /translation="MVYLDNAATTFPKPASVNRAVYRFLTDKAANPGRSGHQLAMASS
                     EEVYRCRKLAAEFFNAPGPECVAFTMNATMAINMALKGRLTGGHVVTSNLEHNSVMRP
                     LSRLAKLGVTFSTAQVDLASDEQTLENIEKLIGRDTVMVACTHASNVCGRSLPIKQIG
                     RLCKSRGIEFLLDASQSAGILPIDMQNCCIDYLCMPGHKSLYGPMGTGMLITSKGETL
                     ETITEGGTGSKSVSFDQPDFMPDRMESGTVNAPGIAGLRAGMNYVRKRGIDTIFREEM
                     KLYLMAYDLLSELDGIRLYNPRPVQGYVPVLGFNIGEMPSEQAVIDLDHLGIALRGGL
                     HCSPAAHQAFGTLSQGMIRASFGAFNRREDVFALTDGVQKIMKRAKIL"
     gene            3708..4565
                     /gene="cdaA"
                     /locus_tag="VHR42_01685"
     CDS             3708..4565
                     /gene="cdaA"
                     /locus_tag="VHR42_01685"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX3025928.1"
                     /translation="MNFINSSLQFFISILNTVTLLDILDIVIVAYLIYKTVQLVRETR
                     AEQLIKGIVLLIIAYYFSMLLNLKALSFILYNIWQFGVIALVVVFQPELRSALEHLGR
                     TSVGNFKIFSFDNTEDGELKNKEALVAVCEAAEVLQKNRIGALILFERKTKLGEIIKT
                     GTVINADISPEILVNVFYPKAPLHDGAMVIRNGKVFAAGCFLPLSQNQEVSRELGTRH
                     RAALGISENSDVLVVVVSEETGIISLVSNGVIKRNLTIDTLRIALESGLLPHKAESVK
                     KSAFRMFGR"
     gene            4570..5829
                     /locus_tag="VHR42_01690"
     CDS             4570..5829
                     /locus_tag="VHR42_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX3025929.1"
                     /translation="MKSKFKLNKIFYNNQILKLFSIVTAILFWFIVASSINPEYSRTI
                     GGVKIQVDTSGKKFSDLGLHVISLSQDQLSVGVTGPRYLIGKLNADDFTAVPDFDSVN
                     KPGTYNLNLDVKFSGSQSNMSISKLKPGTISIRFDTMTTKKVPVKAALDENQLSDGYL
                     AQSAVIEPSSVTITGPTTDVAKVTNAVAKISIPQGTSRTVSGKEKIMLVDSKNKEVST
                     EYISIDQEYAQVSVPVLKYKDVPLAVSFDNLPAGYDKKNISCVFDPTTVRLAGEDDII
                     DGIGQVAVKNIDFLSVGLQKTISCDIQIPDGIVNVNHINKVKLTVTLNDTALKIVNTA
                     SITAANVPSGYTAKLLTDQISGIKLYGLKSAVGQVGSVNAVVDMSKATGGSGYYFLPV
                     TIQSPGSGMWTAGTYTAEVRLKRVSTW"
     gene            5823..7421
                     /locus_tag="VHR42_01695"
     CDS             5823..7421
                     /locus_tag="VHR42_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025930.1"
                     /translation="MVIVNNIRIPLEAEESVAVDLALKRLKLRLSDVQQTCIVKKSVD
                     ARHRSRISLVYSVGIEVEQRHQERLKSFVIDAPDVLIRAQKPYEIKPGKEALKNRPVI
                     AGFGPAGMFAGLILSKMGYAPLVIERGADVDERMAKVDRFWKNGILDTETNVQFGEGG
                     AGTFSDGKLTTRINDPRCETVLNELVRHGAPEEVQRITRPHIGTDKLIGIVKSIRKEI
                     VQNGGEVRFGAKLDKISFQSGKLKSVTLNDGTDIETRTIILAIGHSARDTFEMLSKCG
                     VMLTPKPFSVGVRIEHLQSEIDRGLYGEYAGHPSLPKGEYQLSLRQGNRAVYTFCMCP
                     GGVVVAAASEQGGVVTNGMSYFDRGGPNANAALAVSVGPEDFGNSLFAGVEFQRKLEY
                     EAYRAGNGSFRAPFQTVNAFIGGLDQFPIARVEPTYPVGVEPYRLDRIFPDTVNQMLQ
                     TGLYAFDRRLPGFAAGDAILTGVETRTSSPVRISRNEEFEAVGLHGVYPAGEGAGYAG
                     GIMSAAVDGLRCAEAIIRRYSPIR"
BASE COUNT         1899 a         1719 c         2183 g         1760 t
ORIGIN      
        1 ttttgaagaa gaagtcatgc agtccaaatt accggtgctg attgactttt gggctccctg
       61 gtgcggccca tgcaaaatgg tttcaccgat cgtggacgaa attgccacgg aaggagaagg
      121 cagcctggtt gtcgggaaaa tcaacatcga cgaagagcct gatctggcta cgcggtttaa
      181 tgtgttcagc attccgactc tgattgttgt aaaagacggg gaagtccagg caacctctgt
      241 cggagcgcgg gcaaaagagg atatcaaaca gaccctcggc atctaaaacc gattcagaca
      301 cagctgcgaa aattttcgca gctgtgtttt tttgcgtctg acaggatttc tatcggagta
      361 agaacccggt tggccggaaa tacgatggaa agaccgtctg gccggggtgg cggttttcag
      421 cgacacttgt tataatggat tcatacggaa atggaatcgg aaagtgttag ctgaaggaat
      481 gatattatgg gattgattca gacagtcagg aacagaatgg attactggaa agacagggga
      541 gaggttcctc cggttaccgc aattgttgta gccgccggaa aaggagaaag aatggggggc
      601 gaggccagca aacagctgct gtcggtctgt ggaatgccgg tgatcgccag aacgcttgga
      661 gcttttgaaa actgccggtc ggtgaatgag gtcgttgttg tcgcccggga tgaagatatg
      721 gttgagattt cccggatcgt caaggatttt gaattgacaa aggtcgtgag gatcgtgagg
      781 gggtctgaca cacgtcagcg ctcggcgctt tgcggccttg aagcggccgg agaagcggcg
      841 tattttgcca tccatgacgg ggcccggcca ctcgtttcgc ctgaatgtat cgaccgggtt
      901 gtggaaggcg cccttcgcca cggagccgcc gccgccgccg tccgggtcaa ggataccgtc
      961 aaaatagcgg atgacgaagg ctttgtgctc tcgactcccg accgttcccg actctggagc
     1021 attcagactc cccaggtgtt tgaggccggg ctttaccgcg gcgcggtaaa aaaggcggtg
     1081 gaggagcagg cggattatac cgacgactgc cagatgattg agagcgtcgg gcagaaaatt
     1141 cgtctgattg aaggggagta caccaatctg aagattacca ctccggatga tattctggcc
     1201 gcggaagcct atttgggcga gggcgaggag agtgagacgt tttgagaatc gggcatggtt
     1261 atgacgcaca ccggtttgaa atgggaagaa aattaattct cggcggtgtc gagattgaat
     1321 acgagatggg gctgctcggg cattccgacg cggatgtgct gactcacgcc gtaatggatg
     1381 cgatcctcgg agcggcggct cttggggata tcggacggca tttccccgat accgatgaga
     1441 aatacaaagg ggcggacagc ctggtgctgc tgcgggaagt aatggcgatg gcggagaaga
     1501 agggttaccg gcttggtaat ctcgatgcga cggtcattgc gcaggctccg aaaatcaagc
     1561 cgtatctgga tcgcatccgg cgcaatcttg ccgaatgctg cagagtggat ccggattcag
     1621 tgaatgtcaa agccaccaca gaggaaggta tgggtttcac cggaagactg gagggcgtat
     1681 ctgctcacgc agtttgcctg ctctgtaagc tgccggaatg cgatcgttaa atcgtggcgg
     1741 tttcatagga tccggctgtt tgcccgatcg gtttcgcgcc gatacaggct gatgaaaaac
     1801 tcatggcggc cggcaaagca atgtcgttaa tcagcttttt tgcataagat agaatgctac
     1861 gatcagattg aaatcggagc agagaatctg agacgccggg aggccatgaa agcgagcggg
     1921 ttatgcagcc gggctatttc agttactgga cagcactgtt tctgtgtatt tttccgtgcg
     1981 cttccggcag aaccgtcgcc tgacagaacc gtgaggtgaa accatgagca ggcagtttat
     2041 tgtcgtaagc tcaatcactt acgcttataa gggccgggac gctctccaga ataaaggctt
     2101 cagcgcatat attgaacgcg ctccaaaaga attatcgtcc tgcggttgcc attattgtgt
     2161 ttattttaag aactgcagcg ttgaatacgc aacggaaatt ttaaaaagcg cccatgtcag
     2221 gatcatttca accggagtca cgcagtaatc cggcagatcg ggagttctct gttaacgggg
     2281 ggatactatg gtttatcttg acaacgcggc tacaacattt ccaaagcccg cctcggtcaa
     2341 ccgggcggtt tatcgttttt taacggacaa agcggcgaac ccgggcagaa gcggacatca
     2401 gctggcaatg gcttcctccg aggaggtata ccggtgcaga aaactggcgg ctgagttttt
     2461 caacgcgccg gggcctgaat gtgtcgcgtt cacaatgaac gcgacgatgg cgattaacat
     2521 ggctcttaag ggcaggctga caggcggcca tgtcgtcact tccaatttgg aacataactc
     2581 cgtcatgcgt cctctgagca ggcttgcaaa attgggagtc acattcagca ccgcacaggt
     2641 cgaccttgca agtgacgagc agactcttga aaatattgaa aaacttatcg gcagagacac
     2701 ggtgatggtc gcctgcactc atgcctccaa tgtttgcggc aggagtctgc cgatcaaaca
     2761 gatcgggcgt ctctgcaaaa gccggggaat tgagtttttg cttgacgcgt cacagtccgc
     2821 ggggatcctc ccgattgaca tgcagaattg ctgcatcgat tacctgtgta tgccgggaca
     2881 caagagcctg tatgggccga tggggaccgg catgctgatt acttcaaaag gcgaaacatt
     2941 ggaaaccatc acagagggag gaaccggcag caagtcggtc agttttgacc agccggattt
     3001 catgccggac aggatggaga gcggaacggt caacgctccc gggatcgccg gtctgcgcgc
     3061 cggtatgaac tatgtcagaa aacgcggcat cgatacgatt ttccgggaag agatgaaatt
     3121 gtatctgatg gcctatgatc tgctgtccga acttgacggt atccggcttt acaacccgag
     3181 gcctgttcag ggttatgttc cggtattggg ttttaacatt ggggagatgc cgagcgagca
     3241 ggcagtcatt gatcttgatc acctgggtat tgcgctccgc ggcggacttc actgctcgcc
     3301 agccgcccat caggccttcg gaacactgtc tcagggaatg atccgcgcaa gttttggggc
     3361 gtttaaccga agggaagatg ttttcgcact gacggatggt gttcaaaaaa tcatgaaacg
     3421 cgcaaaaata ctttgaatct tcgctgtttt atggtagaat aataacaatt ggatagaacg
     3481 gccgatttta tgaatgccga atagtcgatt ggctaagcaa acaggagaat gaaaattctc
     3541 ttgtttgacc gtaacaggga caggcattcg cttttacgac gacacccgca tgaatatttc
     3601 gtttcttaac gcccgcatcg gcagggcggg tcaaacatac aaggctgcat gaggctaacg
     3661 gtcgttaaag gcaaagaata ccaaaatacc ggaagggtgc tttgcttatg aatttcatta
     3721 attcctctct gcaatttttt atcagtattt taaataccgt cacattactg gatattttag
     3781 atatcgttat cgtcgcttat ctgatttata aaacggttca gcttgtcagg gagacgcggg
     3841 ccgagcagtt gattaaagga attgttctcc tgattatcgc gtattatttc tcgatgctgc
     3901 tcaacctcaa agcgctttct tttattctct ataatatctg gcagtttggc gtgatagcgc
     3961 tggtcgtcgt gttccagccg gaactgagaa gcgcccttga acatcttgga cggaccagcg
     4021 tcggtaattt taaaatcttt agcttcgata atacggaaga cggcgaattg aaaaataaag
     4081 aggcgttggt ggccgtctgc gaggcggcgg aagtgctgca aaaaaaccgg atcggcgcgc
     4141 tgattctttt tgagcgcaaa accaagctcg gggaaatcat caaaaccggc accgtcatca
     4201 acgcggatat cagccccgaa attttggtaa acgtctttta tcccaaggct ccgctgcacg
     4261 acggggcaat ggtgatcagg aatggaaaag tgttcgcggc gggctgtttc cttccgctgt
     4321 ctcaaaatca ggaggtcagc agggagcttg gaacaagaca ccgcgcggcg ctgggaatca
     4381 gtgaaaactc cgatgttttg gttgtcgttg tttcggagga aaccggaatc atttctttgg
     4441 taagcaacgg cgtgataaag cgcaatctca ccattgatac cctccggatt gccctggaaa
     4501 gcggcctttt gccgcataag gccgagtcgg tcaaaaaatc ggccttccgg atgtttggaa
     4561 ggtgatggaa tgaaatcaaa atttaaactc aataagattt tttataataa ccagatatta
     4621 aagctgtttt ccattgtaac ggcgattttg ttctggttta ttgtggcttc cagcatcaat
     4681 ccggaatact ccagaacaat cggcggcgtt aaaattcagg ttgatacttc gggaaagaaa
     4741 ttttccgatc tgggtttgca tgttatttcc ttatcgcagg atcaattaag tgtcggggtc
     4801 accgggccga gatacctgat cggcaagctg aacgcggatg attttaccgc ggttcccgat
     4861 ttcgacagtg tcaacaaacc gggaacatat aatctgaatc tggatgtcaa attctccggt
     4921 tcgcaatcca atatgtccat cagtaaatta aaacccggaa cgatcagcat ccggtttgat
     4981 acgatgacga caaaaaaggt gccggtgaaa gcggcacttg atgagaatca gctgtccgac
     5041 ggctatcttg cccagagcgc ggtcattgaa ccgtccagcg tcacgattac cgggccgacc
     5101 accgatgtgg caaaagtgac gaacgctgtc gcaaaaatca gcatccctca gggaaccagc
     5161 cggacggtgt ccggaaaaga aaagatcatg ctggtggaca gtaaaaataa agaggtcagc
     5221 acggaatata tttcgatcga tcaagagtat gcccaggtat ctgtcccagt gttaaaatac
     5281 aaagatgttc cgctggccgt ttcctttgat aatttgccgg cgggttatga taagaaaaat
     5341 attagctgcg tgtttgaccc gacgacggtc aggcttgcgg gtgaagatga tattatcgac
     5401 ggaatcggcc aggtagccgt taaaaatatt gatttcctgt cggttggcct tcagaaaacg
     5461 atatcctgtg atattcagat tccggacggc atagtgaatg tcaatcatat aaacaaagtg
     5521 aaactgaccg ttacgctgaa cgacacggct ttgaaaatcg tgaacacggc gtcgattaca
     5581 gccgccaatg tcccaagcgg atataccgct aaacttctga ccgaccagat cagcggaatc
     5641 aagctttacg gcctgaaatc cgcggtcggt caggtcggtt cggtaaatgc ggtggtggat
     5701 atgagtaagg cgacaggggg cagcgggtat tactttttgc cggtgacaat tcagtctccc
     5761 ggttcgggaa tgtggacggc cggaacctat acggcggaag tcaggctgaa aagggtgagc
     5821 acatggtaat cgtcaataat atcaggattc cgttggaagc agaggaaagc gtggcggttg
     5881 atcttgcgct caaacgcctg aaacttcgat tgtccgatgt tcagcagacc tgtatcgtta
     5941 agaaatcggt agacgcgcgt caccgcagcc gtatttcgct ggtttattcg gtgggaattg
     6001 aagttgagca gcgccatcag gaacggctga aatcctttgt cattgatgct cccgacgttc
     6061 tgatccgcgc ccaaaagccg tatgagatca agccgggaaa agaagcgctg aagaataggc
     6121 cggtaattgc cggttttggc cccgctggaa tgtttgcggg gcttattttg tcgaaaatgg
     6181 gatacgctcc tctcgttatc gagcgcggag ccgatgtgga tgaacggatg gcaaaggttg
     6241 accggttctg gaaaaacgga atcctcgata ccgaaaccaa cgtgcagttc ggtgaaggag
     6301 gggcgggcac cttttcagac ggaaaactca ccacacgcat caacgacccg agatgtgaaa
     6361 cggttttgaa tgagcttgtc cggcacgggg cgcccgagga ggttcaaagg atcaccaggc
     6421 cccatattgg gacggacaag ctgattggta tcgtaaaatc cattcgaaaa gaaattgttc
     6481 aaaatggcgg agaggtgcgt tttggggcaa agttggataa aatctcattt caaagcggca
     6541 aactgaaatc cgttaccctg aacgacggca ccgatattga aacgcggacg atcattctgg
     6601 caatcggcca cagcgcccgg gatacgttcg agatgctgtc aaaatgcggg gtgatgctca
     6661 caccgaaacc gttctccgtc ggagttcgga tcgagcacct gcagtcggaa atcgaccggg
     6721 gcctttatgg tgagtatgcc gggcatccaa gccttccgaa aggagagtat cagctttccc
     6781 tgagacaggg gaaccgggct gtttatacct tctgcatgtg ccccggcggg gtggttgtcg
     6841 ccgcggcttc cgagcagggc ggcgttgtaa ccaacgggat gagttatttt gacaggggag
     6901 ggcccaatgc aaatgcggcg ctcgccgtgt cggtcggtcc tgaagatttt ggaaattccc
     6961 tgtttgccgg agtggaattc cagagaaaac tggaatatga ggcctatcga gcgggtaacg
     7021 ggagtttcag ggctccgttc cagacggtaa acgcttttat cggcgggctt gaccagttcc
     7081 caatcgcgcg tgtggaacca acctatccgg tcggagtcga accttatcgg ctggacagga
     7141 tattccccga caccgtaaac caaatgctcc aaacggggct ttatgccttt gacaggcggc
     7201 tgcccgggtt tgccgccgga gatgccatcc tgacaggagt ggagacccgg acgtcatcac
     7261 cggtcagaat cagccgaaat gaagaatttg aagctgtcgg gctacatggt gtttacccgg
     7321 ccggggaggg tgccgggtat gcgggcggca tcatgagcgc cgcggtggac gggctcagat
     7381 gtgcggaggc aatcatcagg agatactcac cgatccggta aaaaatccgt atgatgtaag
     7441 aaagagcaag actgggttgg ttgaggctgt tatcctgggg ggacaaaagt gaaacttgga
     7501 atcgcgctgt ccggcggggg actctggggc gcttattgtg ccggcgtcct ggagggactt
     7561 c
//
LOCUS       DATALY010000057         9394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_272340,
            whole genome shotgun sequence.
ACCESSION   DATALY010000057 DATALY010000000
VERSION     DATALY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272340"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VHR42_01700"
     CDS             <1..538
                     /locus_tag="VHR42_01700"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519125.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX3025931.1"
                     /translation="MESKIPFGLGFVKNRYVGRTFIQPTQGQRDTLVGIKLNALASAV
                     KGKRVVMVDDSIVRGTTSARIVSLLRNAGATEVHVRISAPPFTNPCYFGTDIDSKDKL
                     IACRMTVDEICRSIGADSLGYMKIEDLDHITEGTKCGFCKGCFTGDYPIEVPKEFPKD
                     RFEVKLKEASNCMGHEKP"
     gene            583..1617
                     /gene="purM"
                     /locus_tag="VHR42_01705"
     CDS             583..1617
                     /gene="purM"
                     /locus_tag="VHR42_01705"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX3025932.1"
                     /translation="MKSFSESYKAAGVDVTAGYKAVELIKSHVARTVIPGVISGIGGF
                     GGLFELDFLNYKKPVLVSGTDGVGTKLKIAFLMDKHDTVGVDCVAMCVNDVVCGGAKP
                     LFFLDYIAVGKNVPDKIAKIVSGVADGCVRAGCALVGGETAEMPGFYPPEEYDLAGFG
                     VGIVEKDRIIDGGRVRQGDAIIGLASSGVHSNGFSLVRKIFGLNAGKLSTYVEDLSET
                     IGEALIRPTRIYVKPMLRLMEEVDVHAVSHITGGGFYENIPRMLPEGMKAVIHRDAFE
                     VPPIFHLIGRTGKVPERDMFNTFNMGIGMCAAVAADQADRAVELLRAEGEKASIIGEI
                     VKGDNEVELI"
     gene            1614..2243
                     /gene="purN"
                     /locus_tag="VHR42_01710"
     CDS             1614..2243
                     /gene="purN"
                     /locus_tag="VHR42_01710"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX3025933.1"
                     /translation="MNIAVLVSGGGTNLQALIDAQESGRISNGNIAVVISSKDGVYAL
                     QRAAEHRIPSRVIARKQFSSAEEFDRALLGCLKEYDVGLVVLAGFLSILGDTVTAAYR
                     NRIINVHPSLIPSFCGNGYYGLKVHEAALKYGVKISGATVHFVNEVTDGGAIILQQAV
                     EVFPDDTPETLQLRIMQQAEWKLLPQAVSLFCEGRLTLKDGKVRITQKS"
     gene            2300..3436
                     /locus_tag="VHR42_01715"
     CDS             2300..3436
                     /locus_tag="VHR42_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX3025934.1"
                     /translation="MSDNQENMQNAAGELGIIPMRGCEDILSKVNNYLLEWRKDEPDC
                     PDTYEIEVDCPRFSSGEAKGHIQKSIRGHDLYILCDVFNYGVTYKMYGKQVPMSPDDH
                     FADLKRIIAAASGKARRITVIMPMLYEGRQHRRISRESLDCALALQELQNMGVTNIIT
                     FDAHDPKIQNAIPLIGFDNVYPTYQMIKALVRTVPDIKIDREHLSVISPDEGGMSRCI
                     YYSSVLGLDLGMFYKRRDYSKIVKGRNPIISHEYLGGNIKGMDVCVVDDIIASGDSMI
                     DIAQKLREKQAGRVYLFATFGLFSDGLDRFDEAYKNGLIEKVYTTNLIYTPEELKNRE
                     WYEKVDMSKYIAYIIDTLNYDRSMSDLLQPVKRINALLDRIKNK"
     regulatory      3496..3574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            3640..5190
                     /gene="purH"
                     /locus_tag="VHR42_01720"
     CDS             3640..5190
                     /gene="purH"
                     /locus_tag="VHR42_01720"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX3025935.1"
                     /translation="MKKRVILSVSDKTGVVEFARALEGLGYEIVSTGGTSKALSEAGV
                     EVTGVSDITGFPECLDGRVKTLHPKIHAGILAMRSNPEHVRQIKELGVTPIDIVAINL
                     YPFKQTILKPDVTLEQAIENIDIGGPTMIRAAAKNWQDVAVVVDPADYQMVVDELRAK
                     GEVSRETKFNLAGKVFEHTAAYDALIASYLRRQRNAKGFPDDLTLTFEKVQEMRYGEN
                     PHQPAAFYKEIGNFDNTLAAAEQLHGKELSYNNINDANGALDTLKEFGFDQPCAVAVK
                     HANPCGVGLGEDLYEAYLNAYNSDPVSIFGGIVALNREVDEKTAEELAKIFLEIIIAP
                     SFSDRALEILTKKKNIRLLKLSGVAKANCKDTLDMKKVVGGLLVQKLDTEQLNEAELH
                     CVTRRRPTEQEMKDLLFVWKVVKHVKSNGIALAKNYMTVGVGPGQTNRITALELAIKY
                     AGDRARGSVMGSDAFFPFSDCVEAAVKAGITAIIQPGGSIRDEDSIKAADEAGIAMLF
                     TGMRHFKH"
     gene            5332..6588
                     /gene="purD"
                     /locus_tag="VHR42_01725"
     CDS             5332..6588
                     /gene="purD"
                     /locus_tag="VHR42_01725"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX3025936.1"
                     /translation="MDILVVGGGGREHAVIKKLKQSPHTGTVYCAPGNGGIAQDAICV
                     PVKATDVDAVVALAVEKKVGLVVVTPDDPLMLGMVDALEEKGIRAFGPKKNAAEIEGS
                     KVFSKNLMQRYHIPTAGYHVFDDPAQALAFIREQNSYPTVVKADGLALGKGVVIAETE
                     QEAETALRSIMEDKIFGASGNRVVIEEFLTGPEVSVLSFTDGKTIVPMVSSKDHKRAL
                     DGNLGPNTGGMGTISPNPHYTGEMAEWCMKEIFRPTVEAMNREGRPFKGCLYFGLMLT
                     ADGPKVIEYNARFGDPETQVVLPRLKTDLVEIFDAVIDEKLDSVEIEWDDRAAACVVA
                     ASGGYPGKYETGRKITGLERAEALGNEVYHAGTRMENGSIVTSGGRVLGVTALGEGLD
                     QALTKAYQGLSQIHFEGMYYRKDIGR"
     gene            complement(6669..7376)
                     /locus_tag="VHR42_01730"
     CDS             complement(6669..7376)
                     /locus_tag="VHR42_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3025937.1"
                     /translation="MCLLNADFPRLLTLLRKEKGISQKSAAKELKISQALLSHYEKGI
                     RECGLDFIVRAADFYGVSCDYLLGRSPDRTGAVISVDEIPEPDAAGKENVFKGSLLPT
                     LNKKLIANSLNIIFDLLQKSESRELTSSVSSFLMIAVYKMFRYIYQSNPKNPDGLFAA
                     DRHVFEELSTASMIVSEMKVRCTSEGVRTKGITPFEEPDKLDLSPEKIAGQYPLFAAS
                     LSNLITNAESVMGSRKK"
     gene            7537..8187
                     /locus_tag="VHR42_01735"
     CDS             7537..8187
                     /locus_tag="VHR42_01735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3025938.1"
                     /translation="MKAYHIYLLRHGETQGNREGRYVGRTDLPVSEEGRKRLGELAAH
                     NRYPDAEEIYSSPMERCVETLGMLYPGRKPEVIDDLRECDFGNYEGKTLEQLRDDPEY
                     QKWASGASSAPPGGESSVAFQKRCCAAFEIIVESMMKTGRGSAVVMAHGGTIMSVLAR
                     YAFPRRNFYDWLCESGTGFEVLITPQLWMSGKALEVVGYVPESVEESGGASDEAAE"
     gene            8207..8719
                     /locus_tag="VHR42_01740"
     CDS             8207..8719
                     /locus_tag="VHR42_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX3025939.1"
                     /translation="MGGLIHIYTGDGKGKTTAAVGLALRASGAGKSIVMVQFLKGRDT
                     SELGPLRRLGVRIVRSDVKKFIPDMTGRELDECKTQQNHCFDEAEREAEGCDLLILDE
                     IMGAMSVGMIAPERVENLVRSRQGKTELVLTGRDAPEWLIGLADYVSDIRSVKHPYDR
                     GVPARKGIEY"
     gene            complement(8766..9077)
                     /locus_tag="VHR42_01745"
     CDS             complement(8766..9077)
                     /locus_tag="VHR42_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025940.1"
                     /translation="METAYFERPAEFMVKSRRMAAVARPTEQTKLPGQIKRQSITTKG
                     GHVLADQKKSDPLKPYRHHQPTSYNRNLGYKPSPYNGNQQFKDMIQKAEQVMSDNEFK
                     N"
BASE COUNT         2356 a         2166 c         2641 g         2231 t
ORIGIN      
        1 tatggagtca aaaattcctt tcgggcttgg atttgttaaa aacagatatg tcggcagaac
       61 ctttattcag ccgacccagg gccagagaga cactttggtc ggaattaagc tcaacgcact
      121 cgccagtgcc gtcaaaggca agagggttgt catggtggac gactcgattg tccggggaac
      181 caccagcgcg agaattgtct cgctgctgcg taacgccggt gcgacggaag ttcatgtgag
      241 aatctccgct cctccgttta ccaatccctg ctatttcgga acggatattg attcaaagga
      301 caagctgatt gcctgccgaa tgaccgtcga cgaaatatgc cggtcaatcg gagcggacag
      361 cctcggatat atgaagattg aagacctcga ccatattacc gagggtacga aatgcggttt
      421 ctgcaaaggt tgttttaccg gagattatcc gattgaggtt ccaaaagagt tcccgaaaga
      481 ccgttttgaa gtcaaactga aagaggcttc gaactgtatg ggtcacgaga agccgtaatg
      541 gacagcgcgg ccttttccca acaacaattt caggaggttt caatgaaaag tttcagcgaa
      601 agctataagg cggccggggt agacgttaca gccggctaca aagcggtgga gctgatcaag
      661 tctcatgtgg cccgcacggt gattccgggt gtgatttccg gaataggagg ctttggcggt
      721 ctgtttgagc tggatttcct caactacaaa aagcccgttc tggtttccgg aaccgacggc
      781 gtggggacga aactgaaaat cgcttttttg atggataagc acgacaccgt tggcgttgac
      841 tgcgttgcca tgtgcgtcaa cgacgtcgtt tgcggaggag ccaaacctct ttttttcctt
      901 gattacatcg cagtggggaa aaatgttccc gacaaaattg caaaaatcgt ttccggcgtg
      961 gctgatggct gcgttcgtgc cggatgcgcc cttgtaggag gagaaaccgc ggaaatgccg
     1021 ggcttctatc cgcctgagga atacgatctg gccggattcg gcgtcgggat cgtagaaaaa
     1081 gaccggatca ttgacggcgg cagggtcagg cagggcgacg cgatcatcgg acttgcgtcg
     1141 agcggcgtac attccaacgg attttccctg gttcggaaaa ttttcggcct gaatgccgga
     1201 aagctttcca cctatgtcga agacctttcc gagacgatcg gcgaggcgct gatcagaccg
     1261 accagaattt acgtcaagcc tatgctcagg ctgatggaag aagtggacgt acacgccgta
     1321 tcccatatca cgggcggcgg attttatgaa aatattccgc gcatgctgcc tgaaggcatg
     1381 aaagcggtta ttcatcggga cgcattcgag gtcccgccga ttttccatct gattggcagg
     1441 acgggcaagg ttcccgaaag ggatatgttc aacaccttta acatgggtat cggcatgtgt
     1501 gccgccgtcg cggcggatca ggccgaccgg gcggtggaac tgctcagggc agaaggcgaa
     1561 aaggccagta tcatcgggga gatcgtcaaa ggcgacaacg aggtggaact gatatgaata
     1621 tcgcggttct ggtttccggc ggcggcacga atcttcaggc attaatcgac gcgcaggaaa
     1681 gcggccgtat ttcaaatgga aacatcgcag tggtaatttc cagcaaagat ggggtttatg
     1741 cattgcaaag agccgcggag catcggattc cttcccgggt gattgccaga aagcagtttt
     1801 catcggccga agagtttgac cgcgctctgc tgggctgtct gaaagaatac gatgtcggcc
     1861 tggtggtgct ggcgggattc ctttcgattc tcggcgatac tgttaccgct gcctaccgca
     1921 acaggattat caatgttcat ccctcgctga tcccctcgtt ttgcgggaat ggctattacg
     1981 gcctgaaggt tcacgaggcg gcgctgaaat acggggttaa gatcagcggg gctactgttc
     2041 attttgtcaa tgaagttacc gacggcggag ccattatact gcagcaggcg gtggaagttt
     2101 tcccggacga cactccggaa acgctccagc ttcggattat gcagcaggcc gaatggaagc
     2161 tgctgcccca ggccgtttcc ctcttttgtg agggcaggct gacgttgaaa gacggaaaag
     2221 tgaggatcac gcagaagtcg taatgctgcg gcgaatccga tctggtaatt tattttgaaa
     2281 aagaaagggt catgatttta tgtcagataa tcaggaaaat atgcaaaatg ccgcagggga
     2341 actcggcatt atcccgatga gaggatgtga agacattctt tcaaaggtga ataattatct
     2401 gcttgagtgg cgcaaagacg agcccgactg cccggatacc tatgaaatcg aggtcgactg
     2461 tccccgattc agctcaggag aggcaaaggg ccatattcag aagtccatcc ggggccatga
     2521 cctttatatc ctctgtgacg tctttaatta cggggttacc tacaagatgt acggcaagca
     2581 ggttccgatg agccccgacg atcattttgc ggatcttaag cggattattg ccgccgcctc
     2641 gggcaaagca aggcgcatta ccgtcattat gcccatgctt tatgaaggaa ggcagcacag
     2701 aagaatttcc cgcgaatccc tcgactgcgc tctggccctt caggaactgc agaacatggg
     2761 tgttacgaac atcattacgt ttgatgcgca tgacccgaaa attcaaaatg caattccgct
     2821 gatcggtttt gataacgttt atccgaccta ccagatgatt aaggcgctgg tgcgaacggt
     2881 tccggatata aaaatcgacc gggagcatct ttccgttatt tctcctgatg aaggaggcat
     2941 gtcccgctgc atatattact cctcggtttt gggcttggat ttgggtatgt tttataaacg
     3001 ccgcgactat tccaaaattg tcaaaggcag aaatcccatc atcagccacg aatatctcgg
     3061 cggaaacatt aagggaatgg acgtctgtgt ggtcgacgat attattgcat cgggcgactc
     3121 aatgatcgac attgcccaaa aactccggga gaagcaggcg ggaagagttt atctgtttgc
     3181 aaccttcggt cttttcagcg acggccttga ccgttttgac gaagcctata aaaacggcct
     3241 gatcgaaaag gtctacacca ccaatctgat ctacacaccc gaggagctca aaaaccggga
     3301 atggtatgag aaagtcgata tgtcaaaata tatcgcttac attattgata cgctgaatta
     3361 tgaccgttca atgtccgatc tgctccagcc ggtcaagcgc atcaacgccc ttctggaccg
     3421 gattaaaaac aaataaaaat acttgataac catgcgcacc tgttatatac ttaaaaaaga
     3481 gatactttat aattttatag gccgtatgac tgacggaagt ggaatcacca catggagtac
     3541 ggttttttaa atgccgaccg tctgggcaaa ctgtaattgt ctgggcggtc tttgtctttt
     3601 tttcgcccgg atgagggaaa agctgaagga ggaactaaaa tgaaaaagcg tgtaatcctg
     3661 agtgtttcag acaagacggg cgttgttgag tttgccagag ctctcgaagg tcttggatat
     3721 gagatcgtgt ccacgggggg cacttcaaag gcattgtcag aggccggcgt cgaagtaacc
     3781 ggcgtgtcgg atatcacggg ttttcccgaa tgcctcgacg gacgtgtaaa aacgcttcac
     3841 ccaaaaatcc acgcgggcat tcttgcaatg cggagcaatc ccgaacatgt ccgccagatc
     3901 aaagagctgg gcgtaacgcc gattgacatt gtcgcgatca atctttatcc gttcaaacag
     3961 accatcctta aacccgatgt aaccctcgag caggcgattg agaatattga tatcggcggc
     4021 ccgactatga tccgggcggc ggccaaaaac tggcaggatg tcgcggtcgt tgttgatccg
     4081 gctgattatc agatggttgt ggatgagctc agggcgaagg gtgaagtgtc gcgcgaaacc
     4141 aagttcaatc ttgccggaaa ggtctttgaa cataccgccg cttatgatgc gctgattgcc
     4201 tcctatttgc gccgtcagcg caatgcaaag gggttcccgg atgatttgac cttaaccttc
     4261 gaaaaggttc aggaaatgcg ttacggggag aacccccacc agcctgcggc tttttataag
     4321 gaaatcggaa attttgacaa tacgcttgcg gcagccgagc agctgcatgg caaagagctg
     4381 tcttacaata acataaatga tgccaacgga gcgcttgata cgctgaagga atttggtttt
     4441 gaccagccct gcgcggttgc agtcaaacat gccaatccct gcggcgtagg gctgggagaa
     4501 gacctttacg aggcttacct caacgcttat aattctgacc cggtttccat ttttggcgga
     4561 attgtcgccc tgaaccgcga agtcgacgag aaaacagccg aagagctggc aaagatattc
     4621 cttgaaatta ttattgctcc gtccttcagt gaccgcgcgc ttgaaatatt gaccaaaaag
     4681 aaaaatattc gccttttaaa gctttccggc gttgcgaagg ccaattgcaa ggatacgctt
     4741 gacatgaaga aggtcgtcgg cggcctgctg gtgcagaaac tggataccga gcagctcaac
     4801 gaagcggaac tgcactgtgt gaccaggcgc cggcccaccg agcaggaaat gaaagatctg
     4861 ctttttgtct ggaaagtggt caagcatgtg aaatcaaacg ggattgcgct ggcaaagaac
     4921 tacatgaccg tgggcgtagg cccgggccag accaaccgca tcaccgcgct cgagcttgcg
     4981 attaaatatg cgggcgacag agccaggggt tcggttatgg gctcggacgc gttcttccca
     5041 ttttccgact gtgtcgaagc ggccgtaaaa gcgggtatta ccgccatcat ccagccgggc
     5101 ggctcaatcc gcgacgagga ttccatcaaa gcggcggatg aagccggcat tgcgatgctc
     5161 tttaccggaa tgcgtcattt caaacattaa ttgcgttccg gtttgctgct tttgcggcgg
     5221 cggagattgt tttttcttcc gaaatcggtt attatggaac aaaagttcga tcgaatcagc
     5281 gaggggtgaa tcatggggcg gacgatctgg ggatcgtgcc ccggtaagct tatggatatc
     5341 ttggttgtcg gcggcggcgg aagagaacac gccgtcatta aaaaattaaa gcaaagccct
     5401 cacacgggca cggtttactg cgctccgggc aacggaggca tcgctcagga cgcaatttgt
     5461 gtcccggtca aagccaccga cgtagacgct gttgtcgcgc ttgcggttga gaaaaaagtg
     5521 ggtctggttg tcgtgactcc cgacgacccg ctgatgcttg gcatggtcga tgcgctggaa
     5581 gagaagggca tccgggcttt cgggccgaaa aaaaatgcgg ccgaaatcga gggaagcaag
     5641 gttttttcca aaaacctgat gcagcggtat catattccga cggctggtta ccatgtgttt
     5701 gacgatcccg ctcaggcgct tgcttttatt cgtgagcaaa acagctatcc gacggtagtg
     5761 aaagccgacg ggcttgcgct gggcaaaggc gtagtaatcg ccgagacgga gcaggaggct
     5821 gaaaccgctc tccgctccat catggaggat aagatctttg gcgcttcggg caaccgtgtg
     5881 gtgattgaag aatttctgac aggccccgaa gtatcggtgc taagctttac cgacgggaaa
     5941 accattgtcc ctatggtgtc gtccaaggac cataaaaggg cgcttgacgg gaatctcggc
     6001 ccgaataccg gcggcatggg aaccatcagc cccaaccctc attacacagg ggagatggcc
     6061 gagtggtgca tgaaagaaat tttcaggccc accgtggagg ccatgaaccg ggaggggaga
     6121 ccgtttaagg gctgccttta ttttggtctg atgctgacgg cggacggccc aaaggttatc
     6181 gagtataatg cccgttttgg ggatccggaa acccaggtag tgctgccacg gctcaaaact
     6241 gatctggtgg aaattttcga cgccgtgatt gacgaaaagc ttgactcggt tgagattgaa
     6301 tgggacgatc gggcggctgc ctgtgtggtt gcggcgtccg gcggttatcc gggaaaatat
     6361 gagacgggca ggaagatcac cggtcttgag cgcgcggagg cgttgggcaa tgaagtttat
     6421 cacgccggaa cccggatgga aaacggttcg attgtgacct ccgggggaag ggtcctgggc
     6481 gttaccgcgc ttggcgaagg tctggatcag gcgctcacaa aagcctatca ggggctgtct
     6541 cagatccatt ttgagggtat gtattaccgg aaagacatcg ggagataata aaatgccccg
     6601 gagcgtaaca gcttcggggc attggttttt ctatgagatt ctttgtgcta taataagctt
     6661 gttcggcttt atttcttgcg ggagcccatg acgctctccg cgttggtgat caggttggag
     6721 agggaagccg cgaaaagcgg atattgcccg gctattttct ccgggctgag atcgagcttg
     6781 tccggttctt caaacggggt gattcctttt gtacggactc cttccgaagt gcatcggact
     6841 ttcatttcgg aaacgatcat cgacgcggtc gacagttcct caaagacatg ccgatccgcc
     6901 gcgaaaagcc cgtcggggtt cttcgggttg gactgatata tgtatcggaa catcttataa
     6961 acggcgatca tcagaaaact ggaaaccgaa gaggtcaact cacggctttc cgatttctga
     7021 agaagatcaa aaatgatgtt cagcgagttg gcaatcagtt ttttgttgag agtgggaaga
     7081 aggcttccct tgaatacgtt ttccttcccc gcggcatccg gctcaggaat ttcatccacc
     7141 gagatcacgg caccggttcg gtcgggagaa cgtcccagaa ggtaatcgca ggaaacgccg
     7201 taaaaatccg ccgccctgac gataaaatcc agcccgcatt cccgaatgcc tttttcgtaa
     7261 tgcgaaagca gcgcctggga aatcttaagt tctttggcgg cgcttttctg gctgattccc
     7321 ttttcttttc ttaaaagtgt caaaaggcgg ggaaaatccg cattcaacag gcacaccccc
     7381 aattgttgaa taacattatt gaataacatg tggtataatt atataccagt atttagctgt
     7441 tgtaaaggtg gcaaaaaagt agttttttgc gttgtcggaa aaaatcgaat tgtccggaat
     7501 cggaacgaac ttctgatgaa agacagggag gaacctatga aagcttatca tatttatctg
     7561 ctgcgtcacg gagaaacgca gggaaaccgg gaagggcgct atgtcggcag gacggatctt
     7621 ccggtaagcg aagaaggcag gaaaagactt ggcgagcttg cggcccataa tcgctatccc
     7681 gacgccgagg agatttactc cagccccatg gagcggtgcg tggaaacgct cggcatgctt
     7741 tatccgggcc ggaagccgga agtgattgat gatttgaggg aatgcgactt cggcaattat
     7801 gagggtaaga cgctcgaaca gctgcgggat gatcccgaat accagaaatg ggcgtccgga
     7861 gcgagttcgg cgcctcccgg cggggaaagc tcggtagcat tccagaaacg gtgctgcgcc
     7921 gcttttgaaa tcattgttga gagcatgatg aaaaccggca gaggcagtgc tgtcgttatg
     7981 gcccatggcg ggacgatcat gtcggtttta gcccgatacg ctttccccag acggaatttt
     8041 tacgactggc tctgcgaaag cggaaccgga tttgaagtat tgatcacccc tcagctttgg
     8101 atgagcggca aagcgcttga agtagtgggg tatgttccgg agtctgttga agaaagcggc
     8161 ggcgcatccg acgaagcggc agaataacgg agaggatggc ttgaatatgg gagggcttat
     8221 ccatatttat acgggagacg gcaaaggaaa aacaacggcg gcggtaggat tggccttacg
     8281 ggcttccggc gccgggaaaa gcattgtgat ggtccaattc ctcaaaggac gcgacacaag
     8341 cgaactgggg ccgttaagac ggcttggagt gcgcattgtg cggagtgatg ttaaaaagtt
     8401 tattcccgac atgacaggtc gggaactcga cgaatgcaaa actcagcaga atcactgttt
     8461 tgatgaggcg gagcgggaag cggaaggctg tgatctgctg attctggatg aaataatggg
     8521 tgctatgtcg gtcggcatga tcgccccgga gcgggtagag aacctggtca ggagcaggca
     8581 gggtaagacg gaacttgtcc tgaccggccg tgatgccccg gaatggctga tcggtcttgc
     8641 ggattatgta tccgatatcc gttcggtgaa gcatccttac gacaggggag tcccggccag
     8701 aaaaggaatt gaatattaag aaaacggcct gccgatttcg gcaagccgtt tttccggtgc
     8761 gggcgtcagt ttttaaactc attgtctgac atgacctgct ccgctttttg aatcatatcc
     8821 ttgaactgct gattaccgtt atagggggaa ggtttataac ccagattacg gttatatgaa
     8881 gtcggctggt gatggcggta aggtttcaaa ggatctgatt ttttctggtc tgccaaaacg
     8941 tgtcctcctt ttgtagtaat ggattgccgt tttatctgtc ccggtaattt tgtctgttcg
     9001 gtcggccggg ctacagctgc catgcgtcgt gatttcacca taaactctgc cggccgttca
     9061 aaatatgctg tttccatttt aaaatggccg cacttttatt cttcactttt ttaataaaaa
     9121 tcattcaaca aatgcaaatc agccgaaatc ctgatgacag aatgacaggc tttaagcgga
     9181 tgatcacgat ccgatcaaaa tgatcaggtg tcttaacttt tgattaaaaa aacatccgcc
     9241 tccggttgta accgtgcacc gaaccctgtt aattagacac ggaaaaagca cccaaatcgt
     9301 agaataggtt tagattactc gtgctggcac cgcatttgat acggtgtcag catattttta
     9361 gtctgtatgc gttcatagtt gtagaaatga atat
//
LOCUS       DATALY010000058         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_272362,
            whole genome shotgun sequence.
ACCESSION   DATALY010000058 DATALY010000000
VERSION     DATALY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272362"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1120
                     /locus_tag="VHR42_01750"
     CDS             416..1120
                     /locus_tag="VHR42_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3025941.1"
                     /translation="MVKILLVEDDAALAVGLKYSMEKEGYQVTTAGGVHAGLSEFGQG
                     NFGLVVLDITLPDGTGFDLCRQIRGQSTVPILFLTACDEETDVVMGLDMGGDEYITKP
                     FRLRELLSRINALLRRSTEGKEDKLRSRGYLLKAEEGRLLRGETDIPLTPAECRLAYL
                     LMSNRNCTLTRSQLLAKLWDNAGDFVDANTLTVYIRRLREKIEKDPSNPNIILTIRGI
                     GYQWSDPHPELEGKLS"
     gene            1117..>1939
                     /locus_tag="VHR42_01755"
     CDS             1117..>1939
                     /locus_tag="VHR42_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX3025942.1"
                     /translation="MKQVHLFENSGVRRLFALLAVLTAVYLLTAFFLNQISVSRFEAV
                     LINRESAVAGKLIALHPEQSDEIISAFVTAGNIKDSERGTAAFGRAGYSGQTVPVVFP
                     SIRGELGRITALYTAPALTALIFFGILTFFALSAIYGRVEQAGKKALSVSRGNYGLRL
                     EEDAEGTFARMNYAFNEMSSGVNAGFEKLRQERVFLKNLISDISHQLKTPLATLKMYN
                     EILLQEPLSGAARDFTVKSSEQLERMEWLILGLLKMARMEAGCLEMNLKNANATVI"
BASE COUNT          499 a          487 c          499 g          454 t
ORIGIN      
        1 atattcattt ctacaactat gaacgcatac agactaaaaa tatgctgaca ccgtatcaaa
       61 tgcggtgcca gcacgagtaa tctaaaccta ttctacgatt tgggtgcttt ttccgtgtct
      121 aattaacagg gttcggtgca ccaattggcc ctcagagctg ctttgtttat gattatctcc
      181 agaattcaag caattgcgac aagtaacgtc ttaaaaagta aacggcaatt ggagaggatg
      241 taaaagataa cgcttttctt acaaaactgt aagttttcgc gtctccttcc gggaaagaat
      301 ccatgctata atagaaacaa tgcggaaaaa gaacttaacg actgcgattc agcggaaaac
      361 cgctgctttt gggatacgaa caatctaaat ttcagataaa gagacggtga tcagaatggt
      421 aaagattctg ctggtcgaag atgacgcggc cctcgctgtg ggtcttaaat acagtatgga
      481 aaaagagggc tatcaggtta caacagccgg cggcgttcat gccggcctgt cggaatttgg
      541 gcagggaaac tttggccttg tcgttttgga tatcaccctc ccggacggca cgggtttcga
      601 cctatgccgg cagattcgcg ggcagtcgac ggttccgatt ctgtttctca ccgcctgcga
      661 cgaagaaacc gatgtggtca tggggctgga tatgggcggc gacgagtata tcaccaaacc
      721 gttccggctt cgggaactcc tttcgagaat caacgccctg ctgcggcgca gcacggaagg
      781 aaaggaagac aaacttagat cccgcggcta tctgctcaaa gcggaggaag gccggctcct
      841 tcgcggtgaa acggatattc cgctcacacc ggcagaatgc cgtctcgctt acctgctgat
      901 gagcaaccga aactgcacgc tgacccgttc tcagctgctg gcgaagttat gggataacgc
      961 gggcgatttc gttgacgcca acacgctgac cgtctacatc agacggctcc gcgaaaagat
     1021 tgaaaaagac ccgtccaacc caaatattat tctgaccatc cggggaatcg gctaccagtg
     1081 gagcgatcca cacccggaat tggaggggaa actgtcatga aacaggtcca tctatttgaa
     1141 aacagcgggg tacggcggct atttgcacta ctcgctgttt taaccgccgt ttaccttctg
     1201 accgcttttt ttctcaatca aataagcgtc agccgtttcg aagcggtcct gatcaaccgc
     1261 gagagcgctg ttgccggaaa gctgatcgcc ctccatccgg agcaatcgga tgagattatc
     1321 agcgcattcg tcacagccgg aaacataaaa gattccgaac gcggaacggc cgcatttggc
     1381 cgggccgggt attcgggtca aaccgtcccc gttgtttttc cgtcgattcg cggtgagctc
     1441 ggtcggatta cggcacttta tacggctccg gcgctgacgg ccctgatctt cttcggcatt
     1501 ctgacttttt ttgctctttc ggcaatctac ggccgggtcg agcaagcggg caaaaaggcc
     1561 ctttccgtca gccgtggcaa ttacggactc cgtctggagg aagacgcaga ggggacgttc
     1621 gcgcgtatga attatgcttt caacgaaatg tcgtccggtg taaacgcggg tttcgaaaaa
     1681 ctgcggcagg aacgcgtttt tctcaaaaat ttaatttcag acatctctca ccagctaaaa
     1741 acacctcttg ccacgttgaa aatgtataac gagattctgc ttcaggaacc tctcagcggc
     1801 gcggcgcgcg attttaccgt aaaaagcagt gaacagcttg aacggatgga atggctcatc
     1861 ctcggtcttc tgaaaatggc cagaatggaa gccggctgtc tggaaatgaa tctgaaaaac
     1921 gcaaacgcca cggtcatcg
//
LOCUS       DATALY010000059         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_790146,
            whole genome shotgun sequence.
ACCESSION   DATALY010000059 DATALY010000000
VERSION     DATALY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790146"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VHR42_01760"
     CDS             <1..1139
                     /locus_tag="VHR42_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025943.1"
                     /translation="SFLSLLMMRAVARMRHREVFATIGGILIFVLVFFGQTMLSSRLS
                     SSNSAELVKAMTARGGLVTQASRIYPPSEWLTFALSGNSAPKYFLYLLGLSAAAVYIV
                     IALSSRIYQKGMQVQFESPKGGSRKKYRFSSSSPVITIVKNEWISIIKTPVYAMNTLL
                     GIILAPLVLIMPAFSGGSDTSSMIRLLHLNVGSFVMILIFAGVMSFFGLINPAAATAI
                     SREGKCLWLMKTIPLPPRTQLLGKFWSGYSISALIVLATAVSLAAVYRLSLAVVLVAS
                     LLALALLLTTTAFSLMVDVIRPKLTWSNQTEAIKQNMNSLFAMLIGFLVVVFFVAVSI
                     ALLLLNLPAAVSVIILLALLSGAAIGTTEWMFWLFDRKFYNMSI"
     gene            1236..1886
                     /locus_tag="VHR42_01765"
     CDS             1236..1886
                     /locus_tag="VHR42_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX3025944.1"
                     /translation="MVLLLSQARGAVFDFDGTLADSLGVWKNVDRKFFENRGLPEPDG
                     YEQAIQSMSITETAEYTRALAGLPESCEEIIKEWHELAREEYNENVRLKPGAADFVLR
                     LRNQGLKLALATASCREFYEPVLKSAGIYGCFDAFTTLEEVSRNKSFPDIYEKTAEKL
                     GLKTQECVAFEDILPAVCGAKSCGMKVIGVYDSHSERDSARIKQAADGYILSFKEI"
     gene            2136..2309
                     /locus_tag="VHR42_01770"
     CDS             2136..2309
                     /locus_tag="VHR42_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025945.1"
                     /translation="MEHSYIIMDKSGRILKKGNLAEEEVRMVAKAGFRVFEGEKEITP
                     ILTGLFDRNQDLL"
     gene            2454..2933
                     /locus_tag="VHR42_01775"
     CDS             2454..2933
                     /locus_tag="VHR42_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX3025946.1"
                     /translation="MNLKPGMPAAAAVLRGSVLAPRISGTVEFYPVESGTVVRVDVCG
                     LPVYMPAAEGRQPVGPFGFHIHEGNSCTPADGMNPFSSSGGHYNPARQPHGNHAGDLP
                     VLFSNHGCAHMCVFTDQFRVEEVIGRTVIIHQNPDDFRTQPAGNSGLKIACGVISRI"
     gene            complement(2989..3267)
                     /locus_tag="VHR42_01780"
     CDS             complement(2989..3267)
                     /locus_tag="VHR42_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025947.1"
                     /translation="MQKQTKDPTGPDNACLEKRLTELEREYDSKRDPANELRLEQLNF
                     YLSGLLGRNNLHYLREYADLLIARYNIDAGWFYQKGYEDACRAAKRRS"
     gene            3966..>4194
                     /locus_tag="VHR42_01785"
     CDS             3966..>4194
                     /locus_tag="VHR42_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484628.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX3025948.1"
                     /translation="MPKDRTEVEVDAAILDKQREYQNKILLINRALYRQNSRAQKAMV
                     ATYGCQQNESDSEKIRGMLCNMGYELTDSAEN"
BASE COUNT         1021 a          959 c         1139 g         1075 t
ORIGIN      
        1 cgtcgttttt atcgctgctg atgatgagag cggttgcgcg catgagacat cgggaggttt
       61 tcgccaccat aggcggaatt ctgatatttg tgctggtgtt tttcggccaa acgatgctga
      121 gcagccgact gagctcttcc aacagtgcgg agctggtgaa agcaatgacg gcgcggggcg
      181 gtctggtaac gcaggcaagc cgtatctatc cgccgtccga gtggctgaca tttgctttga
      241 gcggaaattc ggcgccgaag tatttccttt atttgctggg gctgtcggcc gcggcggttt
      301 atattgtcat tgctttgtcg tcgcggatat atcagaaggg catgcaggtg cagttcgagt
      361 cgccgaaagg gggctcccgg aaaaagtacc gtttttcctc ttcctctccg gtaattacca
      421 tcgtgaaaaa tgaatggatt tcgattatca aaacccccgt ttacgcaatg aataccctgc
      481 tgggcatcat tcttgctccg ctggtgctga tcatgccggc gttcagcggg ggaagcgata
      541 cctcgtcgat gatccgtttg cttcatttaa atgtcggaag ttttgtgatg atattgattt
      601 tcgcgggagt gatgtcgttt ttcggcctga tcaatcccgc ggcggcaacc gctatttcgc
      661 gggagggaaa atgcctctgg ctgatgaaaa cgattccgct tccgccaaga acccagctgc
      721 tcggaaagtt ctggtccggc tattccatat cggcgctgat cgtgctggcc accgccgttt
      781 cgctggccgc cgtttaccgg ctgagccttg cggtggtttt ggtggcgtcg ctgcttgcgc
      841 tggcgctcct gttaaccacc accgcgtttt ctctgatggt ggacgtcatc cgacccaagc
      901 tgacatggag caatcagacg gaagcgatca aacaaaatat gaattcactt ttcgccatgc
      961 tgatcgggtt cctggttgtt gtgttctttg tggccgtctc aattgctctt ttactgctga
     1021 atctgcccgc ggcggtatcc gtgatcattc tgctcgcact tctttccggc gccgcaatcg
     1081 gcaccaccga atggatgttt tggctgtttg accggaaatt ttacaatatg agcatttgaa
     1141 tttgtttgat aaaagaccgc aatttatgtt aaaaatcggc aggtttcgaa tggggtcatg
     1201 cgggcccgga aggttgattt tgaaaggata tggttatggt tttacttctc agccaggcac
     1261 ggggcgccgt ttttgatttt gacggaactc tcgccgactc cctcggcgtt tggaaaaatg
     1321 ttgaccggaa gttttttgaa aacagggggc tgcctgagcc ggacggctac gaacaggcca
     1381 tccagtccat gagcattacc gagactgctg aatatacccg cgcgctcgcc gggcttccgg
     1441 aaagctgtga ggaaatcatc aaagagtggc atgaattggc aagggaagaa tataatgaaa
     1501 atgttaggct taaaccgggg gccgcggatt ttgtgcttcg gctcagaaat cagggcctga
     1561 aactggcgct tgcaacggcg tcgtgccggg aattttatga gccggtctta aagagcgcag
     1621 gaatttacgg ttgctttgac gcgtttacga cgctcgaaga ggtaagcagg aataagtcgt
     1681 ttcccgatat ttatgagaaa acagcggaga aacttggctt aaaaacacag gaatgcgtgg
     1741 cgtttgaaga tattcttccc gctgtctgcg gggcgaaatc ttgcggaatg aaagtgatcg
     1801 gcgtctatga cagtcactcc gagcgggaca gtgcgagaat caaacaagcc gccgacggct
     1861 atatcttaag ttttaaagag atatagagcc gctgagaaaa gaaaaagaat gtaaaagctc
     1921 caatcgggcc gtttgggtcc ggctggagca tttttcttat gtaacaaatt gatcataatt
     1981 tgtataaact aataaacagg tctcatttta tatctaaaac aaatataatt attcaatatt
     2041 gattaaataa tttataaaat aactacaaat aatatcgtta ccgtggtaga ataagagtac
     2101 tagcatggaa cggagagagg ttcgggggcg gaaagatgga acacagttat attattatgg
     2161 ataaaagcgg caggattctt aaaaaaggta atctggcgga agaggaggtc cggatggtgg
     2221 cgaaggccgg attccgggtc tttgaaggtg aaaaggaaat caccccaatc ctcaccgggc
     2281 ttttcgaccg aaatcaggat cttttataaa aacagaaccg aaccgcggaa gacaggccgc
     2341 agctttccaa aagctgcggc ctgtcttctt ttgcgtgttt ctgtcaacat tcccgggatc
     2401 ttaaataaaa tgatattgtg ggaaggtggt gaagacttat cgaaagcgga aaaatgaatt
     2461 tgaaaccggg tatgccggct gccgccgctg ttttaagagg cagcgtgctg gctccccgga
     2521 tcagcggcac ggttgaattt tatccggtgg aatccggaac agtggtcagg gtggacgtct
     2581 gcggactgcc cgtctatatg cccgcggcgg aaggccggca gccggttggg ccgtttggct
     2641 ttcatatcca tgagggcaat tcctgtacgc cggccgacgg aatgaatcca ttttcgtcat
     2701 cgggcggtca ttataatccg gcccggcagc cgcatggcaa ccatgcgggc gacctgccgg
     2761 tgctgttttc caaccacggc tgcgcgcata tgtgcgtttt taccgatcag ttccgggttg
     2821 aggaggtaat cggcagaacg gttatcatcc atcagaatcc ggatgatttc aggactcagc
     2881 cggccggtaa ctccgggctg aagattgcct gcggagtgat aagccgaatt taaacagtta
     2941 catgaaaggg gagcttcttg ccgcgatccg gtaaaagctc ccacttgctt atgaccggcg
     3001 tttcgccgcc cggcaggcgt cttcgtagcc tttttgataa aaccacccgg cgtcgatgtt
     3061 atagcgggcg atcagcaggt cggcgtattc ccgcagatag tgcaggttat tccggcccag
     3121 cagaccggat aaataaaaat tcagctgttc gagccttagc tcatttgccg ggtcgcgttt
     3181 ggaatcgtat tcgcgctcca gctcggtcag acgcttctcc agacaagcgt tgtccggccc
     3241 ggttgggtct ttcgtttgct tttgcataat cctcgctcct ctgttgattt aacggatttg
     3301 ggctcgaaaa agcgggcgca ggtatttttt cgtaccctac acccgctctg acatacaaac
     3361 ccaagcccgt cagatttgca aaaaaggtga agggataata ccgtcgggtt ccctttcatt
     3421 tgcttgcaaa gaaacggcta aacgtttata ataaataacg tcggagcgcc ggccaaaagc
     3481 cggttatgta ggtggctgtt cacctgcaca ggcctgatcg tgttccagca catcaggctg
     3541 cttcggccct gttcttttcg ggccgcagtt ccgttaagat tattataaac cagtaaaacc
     3601 ggttatgcaa gatataattc cctgcgattg gtaagattgg taaatatgtc agggttcaat
     3661 aggggctgtg tgccggttag cggcatgaag ctgcatgaag caggaagcgc gccgtccggc
     3721 ccgcttcttt ttttacagga aaagacaaaa aacgccggac cggggcaaaa ttctctctca
     3781 accgcaagat tcggcagttt cactcaaaat ctttacaaca tattgtgaac gtggtatcat
     3841 aagattgccg tttttgaggc aaaatttcat cggaatatcg ttttaaaatt gccaattgca
     3901 gtttttcttt ttcaaggaag aattgctgta aataacaggg gaaccgtaac tttggaggtg
     3961 tcgctatgcc caaagaccgg acggaagtcg aagtagacgc cgccattctt gacaagcagc
     4021 gggagtatca aaataaaatt cttctgatca accgggcgct ttaccggcag aactccaggg
     4081 ctcaaaaagc gatggtagcc acctacggct gtcagcagaa tgaaagcgac agtgaaaaaa
     4141 tccgtggaat gctctgcaat atgggctatg agctgaccga ttcggcggaa aacg
//
LOCUS       DATALY010000060        12431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_12082,
            whole genome shotgun sequence.
ACCESSION   DATALY010000060 DATALY010000000
VERSION     DATALY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12082"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            733..930
                     /locus_tag="VHR42_01790"
     CDS             733..930
                     /locus_tag="VHR42_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX3025949.1"
                     /translation="MNAGTIVWYNENQGFGFISPDGGGNKLYFNSSQRDSLTVGQEVT
                     FDIKINPNNKRQIAVNVVPKA"
     gene            complement(1002..1229)
                     /locus_tag="VHR42_01795"
     CDS             complement(1002..1229)
                     /locus_tag="VHR42_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025950.1"
                     /translation="MELQRAKLENIKTRLNHGEKVKSTRVESGQFYVQLTNGPEITFD
                     DNYKKQVENTINKANRGIRENTPGPDKTLND"
     gene            1460..2335
                     /locus_tag="VHR42_01800"
     CDS             1460..2335
                     /locus_tag="VHR42_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3025951.1"
                     /translation="MVIKLNYKIITVIFLILFILFLSPSLLNIGKHPVTAAETSTDAP
                     IRIPIILYHEIRPSHLNNMSITPWEFESDLKFLKKSGYTAINMSDLIGYVNGKGSLPD
                     HPVMITFDDGYLNDYVYAYPLIKKYGVKIVLSVIGKNTEDFSNVSDSNLDYAHVTWKQ
                     LNEMSDSGFIEIQNHTYNLHSITRKRFGCEKSKSESVGQYQDLLRNDLSKCQNLISAN
                     TGQMPTTFAYPYGSISKEALPVIKSLGFQATFSCTYGINKITKDPAELYGLKRITRYH
                     GRPLQRTMKECDKLK"
     gene            complement(2467..4284)
                     /locus_tag="VHR42_01805"
     CDS             complement(2467..4284)
                     /locus_tag="VHR42_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX3025952.1"
                     /translation="MTLKKTAASQNVAGGAKNFGDVQKSRAHKIASEVILKFYRISYK
                     IGKLAFCAAGAAGSATRSAGRLLLSEGAAGILSFLRKCSEFKRSRRGRGELSLAKAAS
                     AAALGAAGVLRQTARTIVSEGPVEGFRFFGRLIRRKVSSFWAKNRRMFNYAAPLAGMA
                     VFAVTVYFWSNMSFALSVTYNNQQVGLIKNEQEFRNVAYQMETNVSTAAGRNFTLTQE
                     PQFRLVLVKNSELSTKDDLYDNLVLASSENVVKGYGLYVDKRLIGASEDGAAMQTMLD
                     SILAPYKENPKVQKVEFAQDVEIINGVYPATVVKPVENMLRLMTSSVEGQKAYTVKKG
                     DSPLKVAAMFHLSVNQLYVMNPALTSDQMVQGQTVMVKNARPFLTVRLIVNEAFNEKI
                     PCPVQTVETDSLYQGQTQVKQNGADGIRRIVAAVAYEGKSVAGKIVLSSSVLQQPVEQ
                     IVYVGTKKRPQGSGGDDSGGAQALSASGSREAGGIVAYAEQFMGTRYVMGGAAPGGFD
                     CSGFTKYVYSKYGVSLAHSALIQSYQGSSVGRSGLQAGDLVFFDTNGGGGSINHVGIY
                     VGGGRFVQACSRNPNAITVSSLNERYYQRTYVTARRILN"
     gene            complement(4546..4845)
                     /locus_tag="VHR42_01810"
     CDS             complement(4546..4845)
                     /locus_tag="VHR42_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HEX3025953.1"
                     /translation="MQFNINNQQGQQGQQQMGDMEMMNDSIASQKLISSTYDVYANEC
                     ATPNLRDEFLNIFKDEHQIQADIFTEMQKRGWYQIKPADQQQVTQAKQKYQNMLG"
     gene            complement(5159..5533)
                     /locus_tag="VHR42_01815"
     CDS             complement(5159..5533)
                     /locus_tag="VHR42_01815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX3025954.1"
                     /translation="MTVHRGDIYYADLSPVVGSEQGGVRPVLIVQNNIGNKYSPTVIA
                     AAITSQTGKTNLPTHIRLNAEKCGLSKDSVVLLEQIRTLDKKRLKERMGSADNLSMRM
                     VNEALSISFGLESGEDSGVSFT"
     gene            5841..6335
                     /locus_tag="VHR42_01820"
     CDS             5841..6335
                     /locus_tag="VHR42_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX3025955.1"
                     /translation="MANLFKVNGKFSLSKLAGALAITLGAGAVAGLMSMNAAAIYAAL
                     DLPSFAPPTWVFMPVWTVLYLLMGLALYRILMYEPTPDRKAATFYYSLQLIFNLLWSL
                     LFFVLQLRLAAFVGLVILFFYIVVTTVKFFKIDKPSGFLMVPYLLWVVFAGILNFAVV
                     MLNG"
     gene            complement(6592..7425)
                     /locus_tag="VHR42_01825"
     CDS             complement(6592..7425)
                     /locus_tag="VHR42_01825"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3025956.1"
                     /translation="MLVNVLLLIKSVIMGIVEGVTEFLPISSTGHLILVGHLIGFESA
                     FAKQLFEIVIQLGAILAVVVLYRKKIFKSFHSLRPGGEGFRLWSGIVLAFIPSGIVGV
                     LFNSKVEKYLMRPVTVALALLVGGLWMIYAERRYRKNSRVKRIDDVSYRQAFVIGLFQ
                     CLSILWPGFSRSASTIIGGWIMGLSTVAAAEFSFFLAIPSMFCASGYSLVKSFKTGMV
                     LSGWEVLALIIGFVVSFLVALIVVKKFIDFLKHRPMKGFAYYRIVVGVVILVMVLFRL
                     F"
     gene            7744..8655
                     /gene="nadA"
                     /locus_tag="VHR42_01830"
     CDS             7744..8655
                     /gene="nadA"
                     /locus_tag="VHR42_01830"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX3025957.1"
                     /translation="MDNNSLINEIKRLKRERNAVIVAHNYQNDEVQDIADAVGDSFYL
                     SRFCAQNKSGTIVFCGVRFMAESAKILSPYKTVLLPENQAGCPMADSIIPEDVRRLKL
                     EHPGAAVVCYINTSAEVKAECDICCTSSNAVKIVRSVREKDVIFIPDKNLGQYVAGKV
                     PEKNLILFDGCCVTHSRFGVKEIEVAKGLYPEALTAVHPECPPEVVEKSDFVGSTSEI
                     IHFCKTNPCNEFIIGTEMGILHKLKQDSPGKKFYLLTPRLVCSNMKMTTLNSVHRSLN
                     ELKNVIEVDAEVSAKAVTCLQRMLDAK"
     gene            8685..9776
                     /gene="mnmA"
                     /locus_tag="VHR42_01835"
     CDS             8685..9776
                     /gene="mnmA"
                     /locus_tag="VHR42_01835"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX3025958.1"
                     /translation="MNKTVIAAMSGGVDSSVAAALLKEQGYHVIGITLNVWPSEESKL
                     ACSRTCCSVTDVDDARQVCSKLGIPHYVLNMRDYFKEHVIDYFISEYLEGRTPNPCIA
                     CNAHVKFESLMQKAEALGADYLATGHYANIIKRENGEFCLKRSEDVRKDQTYVLYMLG
                     QRQLSRLLLPCGGYIKPDIRRIAEKYDLPTSSKADSQDICFVGEEGYSGFIRSNCGGA
                     ARPGKVVDKSGKILGDHDGIFNFTIGQRKGLGKYANERKYVIRIDAQTATVYLGDHED
                     LFSPAMLVEKIHHVTSDGIVNGKRMTVKIRYSAPEADARIELSESGTTAKVIFDQPQR
                     AVTPGQAAVFYQDGMVMGGGIIAHPIPLF"
     gene            complement(9852..10004)
                     /locus_tag="VHR42_01840"
     CDS             complement(9852..10004)
                     /locus_tag="VHR42_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025959.1"
                     /translation="MAKAGMRRPDPSEPHGTESNHKQKFPKNSVKPVPEIQGQAKSGK
                     QKAKPA"
     gene            complement(10056..10367)
                     /locus_tag="VHR42_01845"
     CDS             complement(10056..10367)
                     /locus_tag="VHR42_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3025960.1"
                     /translation="MDQSLQQYIKPELLVIIPVLNVLGSFIKRTKIPDWKIPFILGVV
                     SILLSTLWVFSKSPVTGLRDVLYALFVGVTQGILTSGTSVYLNQLVKQGKEGNVTKKD
                     T"
     gene            complement(10530..11321)
                     /locus_tag="VHR42_01850"
     CDS             complement(10530..11321)
                     /locus_tag="VHR42_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3025961.1"
                     /translation="MQKIFDIHTHAYPEKVAEKAVDFLNQYYPVQCKGDGTIGDLAAS
                     AREGGVDYLLVHAVATKPNQVENINDWISEQLTDRIFGFGTIHPHYENDIVGEINRIR
                     SLGLRGIKLHPDFQGFFVDDPATDPIFRTAQGVLPVLIHAGDINSEFSSPSRIARVLE
                     KYPGLTVIAAHLGGYSEWDEAEKNLIGKKVYIDTSSSLWALPPERSVNMIRAHGVDRV
                     LFGTDYPLTRHREELERFYALGLTEEENRKILFENASRLLNLDLN"
     gene            complement(11509..12267)
                     /locus_tag="VHR42_01855"
     CDS             complement(11509..12267)
                     /locus_tag="VHR42_01855"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEX3025962.1"
                     /translation="MELIIKKHSRIDLLDAVRGIAILAMVFYHALFDLQDVFQINTHI
                     FSMLEIFEPPFAGAFILLAGVSCRFSHNNYLRGLKVLALAMAVTVVTVLLIPGQSIYF
                     GILHFMGVSILIYALFGRWIEKIPVRAGLVLWGLLFVLTCLLPLTGSIGIPGLWMLVI
                     PGSFYSIPGSFLFGLPGPDFYSADYFPLIPWIFLFLAGAAAGLPIRQNKLPEKFYTMK
                     VPFLAAVGRNTLLIYALHQPVIYGLLFLFVKGTK"
BASE COUNT         3382 a         3139 c         2662 g         3248 t
ORIGIN      
        1 gcttgacgtg caaagggtca ggggttcgag tcccttagcg cccaccaata caaaccgctt
       61 aggtagttta cctaagcggt tttgttttgc ttataaaccg cgggtcatct ggcaaatcag
      121 aagcctcacc cttcgacgcc atcgccggcc cggagaaata aaagaagaag cctcctaccg
      181 agtctgatgc tgtgttacca caaccccaaa cctcaacgcc cgaaacgagc ggtaggccag
      241 cttctaatat cgggtatatc acgaaatggt gaaaaatata atgccgactt aaattcccat
      301 ttaacctgct tttttgtcaa ttaatcgatt cgcactatcc gaattatatt caaaaggatt
      361 tttgattagt ttatggcact tttcacagac aatccaaaaa ccatccgctt caagttctga
      421 gattgacgat cgcttgcatt ggcagaattt aatctttatt ggcatttttg ttcctcgcta
      481 tccgattgtg tatttggtag tagacaatat aaatacgatt gaagtcacct aatccgtgca
      541 aaataaatta aaaaaaggtg aggtattata tgccaattaa aatgagcgcc gatcaagcgg
      601 caaatagaat cgaaacgctt gcaaaatctg tacttgatga tggaaatata cggggatcat
      661 cgggattggc ggcatcttgt caatttgaaa acgaatacga tatttgttat actggcggtg
      721 gaggtgcgtt atatgaatgc tggtaccatt gtgtggtaca acgaaaatca gggttttggc
      781 tttatatctc ctgatggtgg cggaaacaag ctgtatttca actcatcaca gagagattct
      841 ttgacagttg gacaagaggt aacttttgac ataaagataa atcccaataa caaaaggcaa
      901 atcgcggtca acgttgtacc taaggcataa tctgaaagcc atctcatacg agatggcttc
      961 ttttataaac cccagccctg tgcaggacgc tgctgtttta tttaatcatt cagcgtttta
     1021 tccgggcccg gtgtattttc acggatgccc ctatttgctt tatttatggt attttcaacc
     1081 tgtttcttat aattgtcgtc aaaggtaatt tccgggccgt tcgtaagctg cacataaaac
     1141 tggccgcttt ctactcgtgt gcttttgacc ttttctccat gattcaatct ggtttttata
     1201 ttttcaagtt ttgctctttg taattccata gaaatccctc ctgtaattat tgtacccgga
     1261 ggaagtcata ataaaagatc ctgcccgaaa gtacgcaccg ctttaaacga ttgcaatgac
     1321 aattcttaag aattgtcatt gcttttgttg aatataattg atactgactc cgaaaacggt
     1381 cgatgtagcg gcagaagagc acacatatcg aaaaagtgat gtctgcttca attatcaaca
     1441 atgaaaagga agcattggaa tggtcattaa actgaactat aaaattataa ccgttatttt
     1501 tttaattctg tttatcttat ttctatcccc ctctctgctg aatatcggaa agcatccggt
     1561 cacggcggct gaaacctcaa cggatgcccc catccggatt cccatcattc tttatcatga
     1621 aatcaggccc tcccatctta acaatatgag catcacaccc tgggaatttg agagtgattt
     1681 gaaatttctt aaaaaaagcg gatatacggc catcaacatg tcagacctga tcggctacgt
     1741 caacggaaag ggaagccttc cggatcatcc cgtgatgatc acttttgacg acggctatct
     1801 gaacgattac gtttacgcct accccttaat taaaaaatac ggggtcaaga ttgttctttc
     1861 cgttatcgga aaaaataccg aggatttttc caacgtcagt gacagtaacc tcgattacgc
     1921 ccacgtcacc tggaaacagc tgaacgaaat gtccgattcg ggttttatcg aaattcaaaa
     1981 tcacacttat aatcttcatt ccatcacacg caaacggttt ggctgtgaaa aatcaaaatc
     2041 tgaatctgtc ggccagtatc aggatctgct ccgaaacgac ctgtcaaaat gccagaacct
     2101 tatttctgcg aataccggcc agatgcccac cacttttgcc tacccttacg gaagtatcag
     2161 caaagaagct ctcccggtca ttaaaagcct tggctttcaa gctaccttct cctgcaccta
     2221 cggaatcaac aagatcacca aagaccctgc agagttatac ggtctgaaaa gaatcacccg
     2281 ctatcacggg cggccattac agagaactat gaaagaatgt gacaaactga aataatccgg
     2341 agccccacct catgtgtccg ctaaatgccg cgtgatcggc ccagtttaac aatcgcactt
     2401 tactcacgcc gtcaaagcaa aaaagtcacc ggaaaaccgg tgactttttt aataacaaaa
     2461 cgaattctaa ttcaaaatgc gacgcgcggt cacataggtt ctttgataat aacgctcatt
     2521 cagactgctg acagtaatcg cattcgggtt tctggagcac gcctgaacaa acctgccgcc
     2581 tccgacataa atgccgacgt ggttgatcga acctcccccg ccgttagtgt caaagaacac
     2641 cagatcgccg gcctgaagcc cgcttcttcc gaccgaactc ccctgatacg actggatcag
     2701 tgcagaatga gccagtgaca cgccgtattt actgtataca tactttgtaa aaccggagca
     2761 gtcaaacccg ccgggtgcag cgccgcccat gacgtaccgg gttcccataa attgctccgc
     2821 ataggctaca atgcctcccg cttcccgcga tccagaggcg gagagagcct gtgcaccgcc
     2881 ggagtcatca ccgccgctgc cctgaggcct ttttttcgtt cccacataaa caatttgttc
     2941 gaccggctgc tgaagaacgg aggacgacag gacgattttc ccggcaacac ttttgccttc
     3001 ataagcaacg gcggccacaa tcctcctgat tccgtcagcc ccgttttgct tcacctgagt
     3061 ctggccctga taaaggctgt ctgtctccac cgtctgcacc gggcaaggaa tcttttcatt
     3121 aaaggcttca ttgacaatca gcctgactgt caaaaacggt cttgcgtttt tgaccataac
     3181 cgtctggccc tgaaccattt gatccgaggt aagcgccgga ttcatcacat agagctggtt
     3241 gaccgaaaga tgaaacattg ccgcaacttt taacggagaa tcgccttttt ttaccgtata
     3301 ggccttttgc ccctccacgc tggaagtcat cagcctgagc atattttcaa caggtttgac
     3361 caccgttgcg ggatagacgc cgtttatgat ctccacatcc tgcgcaaatt ccactttctg
     3421 aactttcggg ttctctttat atggggcaag aatgctgtca agcatggtct gcattgccgc
     3481 gccatcctca ctcgcgccga tcagccgctt gtcaacatat aatccgtagc ccttgaccac
     3541 attttcggag gatgccagta caaggttgtc atacaaatca tcctttgtgg ataattcaga
     3601 gtttttcacc agcacaagcc ggaactgcgg ttcctgagtc aaagtaaaat ttctgccggc
     3661 cgctgttgaa acattcgtct ccatctggta ggccacgttt cggaattcct gctcattttt
     3721 aatcaaaccg acctgctgat tgttgtaggt gaccgacaag gcgaaactca tattggacca
     3781 gaaatagacg gtaaccgcaa atactgccat tcctgccagc ggagcggcat aattaaacat
     3841 ccttctattt ttcgcccaaa acgaggacac cttccggcgt atcaacctgc caaaaaaccg
     3901 gaatccttcc accgggcctt ccgatacgat cgtccgggct gtctgcctga gcacaccggc
     3961 agctccaaga gccgcggccg atgcagcctt cgccagagaa agttctcccc gtccccgccg
     4021 cgaacgttta aactccgagc acttgcggag gaaactcaaa attcctgcag cgccttcgga
     4081 cagcagcaat cttccagccg aacgggttgc cgatccggca gcgcccgcgg cgcagaacgc
     4141 cagcttacct attttgtatg atatcctata aaatttaaga ataacttccg atgctatttt
     4201 atgagctctg cttttttgaa cgtctccgaa gttttttgcc ccgccggcca cattttgtga
     4261 agccgcagtc ttcttcaacg tcatagcatc atcacctttc tgttcaacga tcaaatggtt
     4321 acaaacttgt aacaaccatt tattagtata ctacaatttg taacttttac aagacccgaa
     4381 cggaaagaat tcctctttta accgtaattt gtaattcccg ttaagttatc cggctgattt
     4441 ttagatagta tttacaaact gtcagacatg atttttcggt aatgtgtata ctcataacaa
     4501 cagaaagccg caggtatcac ctgcggcttt ctgaacggat attaattagc caagcatatt
     4561 ctggtatttt tgttttgcct gcgtaacctg ttgctgatcg gccggtttga tctgatacca
     4621 tccgcgtttc tgcatttccg taaaaatgtc ggcctggatc tgatgttcat ctttgaagat
     4681 gttgaggaat tcgtcgcgaa ggttcggggt tgcgcactca ttggcgtaaa catcgtaagt
     4741 ggaactgatc agtttctgcg acgcgatcga atcgttcatc atttccatgt cgcccatctg
     4801 ctgctgcccc tgttgtccct gttgattatt tatattgaac tgcatcgttt tttctacctc
     4861 ctttaactta aatgcgtcat caaacgattg aaatgatcct gatgccttgc tgagatctgt
     4921 tcacacttgg atttaagctg cgggtccgta cactgcgttg aaaacgaacg gtatttctta
     4981 accaggatct gctcgtaatt caactgatct tccaatgccg acaattcttt tgaagttaga
     5041 tttggcatat taacactcct ttttgatttt tcataggtat ctttcccttt tgacttcata
     5101 atattctata aaattaacca aatggcgcac gcttttcata cgaagaagtt tgggcggttt
     5161 atgtaaaact gacacccgag tcctctccgc tttccaatcc aaaactaatt gacaacgctt
     5221 cattcaccat cctcatggat agattgtcag cgctgcccat ccgctctttc agccgtttct
     5281 tatcgagtgt tctgatttgt tcgagcaaaa ccacagaatc cttcgaaagg ccgcattttt
     5341 ccgcattcag tctgatatgt gtcggcaaat tcgtcttgcc cgtctggctg gttatggccg
     5401 ccgcaattac ggttgggctg tatttgtttc caatattatt ctgcacaatc agaacaggcc
     5461 gcactccgcc ctgctcagac cccacgaccg ggctcaaatc cgcgtaataa atatctcctc
     5521 tgtgtactgt cacacctgcc acgctccgat tattatgata ttttcatttc acccggcgca
     5581 accgggcttc tgttttattt ttccgcactt tttctaaaat taatcattta attttgaatt
     5641 ttacagaata ctgccggctg actcaccatt cattctggca tccgtcattc tattttattc
     5701 aaaagttcgg aaaaagggga caactaccgt gagctcataa aaggcgcaat ttaaaatagt
     5761 cccgcctgat cgaatgaaaa tgaaaaaaac acatatattt atattaataa gcaacagtaa
     5821 aattttcggg ggaaatgaat atggccaatt tatttaaagt caatggcaaa ttcagtttat
     5881 caaagcttgc cggcgcgctg gcaattacgc ttggcgcggg cgcagtcgca ggactgatgt
     5941 ccatgaacgc agcagctatt tatgcggcgc tggatcttcc ctcatttgct ccgcccacct
     6001 gggttttcat gcccgtctgg acggttctct atctcttaat ggggcttgca ctttaccgca
     6061 tcctaatgta tgagcccaca cccgatcgaa aagccgccac attttattat tcacttcaat
     6121 tgatctttaa cctgctctgg agcctgctgt ttttcgtctt acagctgcgc cttgccgcct
     6181 ttgtcggcct tgtcattctg ttcttctaca tagtagtcac gaccgttaaa ttctttaaaa
     6241 ttgataagcc atccggcttc ctgatggtgc cttatctgct ttgggtcgtt tttgccggaa
     6301 tacttaattt cgcggtcgtg atgctaaacg gttagcaggc tattcaatca ttctgaataa
     6361 ttcataaaca acctgtcaca ataaatgtgt gctaaaattg caattgagaa attttttgtc
     6421 aattcatttt agcgcttact ggcaattggg tgtgtcttaa gctgctttca atgcccggca
     6481 aaatcaacac agaaacaacc atgcgccaga caatacccaa aacaaaaagc ccggttcaga
     6541 cgcacacgcg cctgggccgg gcttttgacc gatttctcac cgattgattt tttaaaacag
     6601 tcgaaaaaga accatgacca atattacaac acccacgacg attctataat acgcaaaacc
     6661 tttcatcggc cggtgtttga gaaaatcaat aaacttcttt acaacgatta atgccaccag
     6721 gaaagacacg acaaatccga tgataagggc aaggacctcc cagccgctca aaaccattcc
     6781 tgttttgaac gatttcacaa gcgaatagcc cgaagcgcag aacatcgagg gaatggcaag
     6841 gaaaaacgaa aattccgccg ccgcaaccgt ggagagaccc atgatccagc cgccgataat
     6901 ggtggatgcc gatctggaaa atccgggcca cagaatggaa aggcattgaa ataaaccgat
     6961 tacaaaagcc tgacggtaac tgacatcgtc aattctcttc accctgctgt tttttcggta
     7021 acgacgctcc gcataaatca tccacaggcc gccgacgaga agggcaagcg ccacggtaac
     7081 cgggcgcatc agatattttt caaccttgct gttaaacaga acgcctacga tacccgatgg
     7141 gatgaacgcc aggacaattc ccgaccagag gcgaaatcct tctcctcccg gacgaaggct
     7201 gtgaaacgat ttaaaaatct ttttgcggta aagcaccacg accgccagaa tggccccgag
     7261 ctgaattaca atttcaaaaa gctgttttgc aaaagcgctt tcaaaaccga tcagatgacc
     7321 caccaatatc agatgtccgg tggaggaaat gggtaaaaat tcggttacgc cttccacaat
     7381 gcccataatg acggacttaa tcagaagcag tacatttacc agcatatcaa aaccccttaa
     7441 tcttttttgc cgcaggacgc ccgccgtgca gggcctccat actccataac agctgaaacg
     7501 atcaatattg cttttatttt aaggcaaata aatttaagat gcaagcgatt ccgtaatttg
     7561 attatgaaaa acgatctgtc cggaattgca aaaaacattt tttgtgatat aatgagcaag
     7621 gctatttatt gtacttcaga cggatcacga atgaaaaaag ctgttttggt taagccaatt
     7681 aataataaca gaaaacaata ttactactgc atgttactac tgtatggtat ggggatgaac
     7741 ggaatggata ataacagcct gatcaatgaa atcaaacgac tcaaacggga acggaacgcg
     7801 gtaatcgttg cccacaatta tcaaaatgac gaagtgcagg atattgccga cgcggtgggc
     7861 gattcgttct atctcagccg cttctgcgcc cagaacaaat cgggaaccat tgtattctgc
     7921 ggggttcgat ttatggcgga aagcgcaaag attctttcac cgtacaaaac ggtgcttttg
     7981 cccgaaaatc aggccggctg cccgatggcc gactcgatta tcccggaaga cgtccgccgg
     8041 ctcaagctgg agcatccggg ggcggccgtc gtgtgctaca ttaacacttc cgccgaggtt
     8101 aaggcggaat gcgatatttg ctgcacttct tccaacgcag ttaaaattgt ccgctcggtc
     8161 agggaaaagg acgttatttt tatccccgac aaaaacctgg ggcagtatgt ggcgggcaaa
     8221 gtgcccgaaa aaaatctgat tttatttgac ggctgctgcg taacccacag ccgtttcggc
     8281 gtaaaggaaa ttgaagttgc caaaggcctg tatcccgagg ctctcaccgc cgtccacccc
     8341 gaatgtcccc cggaagtcgt tgaaaaatcc gattttgtcg gcagcaccag cgaaattatt
     8401 catttctgca aaaccaaccc gtgcaatgag ttcattatcg ggactgagat gggtattctt
     8461 cataaactga agcaggacag ccccggcaag aaattctatc tgctcactcc ccggcttgtc
     8521 tgcagcaaca tgaagatgac gacgctgaac agcgttcacc gttcgttaaa tgaattaaaa
     8581 aatgttatcg aggtcgacgc ggaggtcagc gcgaaagctg ttacctgcct gcaaagaatg
     8641 ctcgacgcca aataattgtt ttcattatta aacgggagga tcctatgaac aaaacggtca
     8701 ttgcggccat gagcggcgga gtcgacagct cggttgcggc ggccctttta aaagaacagg
     8761 gttatcatgt catcggcatc accctaaacg tttggccgtc ggaggaatcc aagctggcct
     8821 gttccagaac ctgctgctcg gtcaccgatg tcgacgacgc acggcaggtc tgctcgaagc
     8881 tcggcattcc gcactacgtg ctgaatatgc gtgactattt caaggaacat gtcatcgact
     8941 actttatttc cgaatatctg gaaggccgca caccaaatcc ctgtatcgcc tgcaacgctc
     9001 acgttaaatt tgaatccctc atgcaaaaag ccgaggcgct cggtgcggat tatcttgcga
     9061 ccggccacta cgcaaacatc atcaaaagag aaaacgggga attttgttta aagcgttcgg
     9121 aagatgtccg gaaagaccag acctatgtgc tgtacatgct gggtcagcgg cagctttcac
     9181 gtctgctgct gccctgcggc ggttatataa agcccgacat ccggcgcatc gccgaaaaat
     9241 atgatcttcc cacctcttcc aaagccgaca gtcaggatat ttgctttgtc ggggaggaag
     9301 gatattccgg gttcatccgc agtaactgtg gcggcgcggc ccggcccggg aaagtcgtcg
     9361 ataaaagcgg taaaattctc ggcgatcatg acggtatctt taacttcacc atcggccagc
     9421 gaaaaggtct gggaaaatac gcgaacgaac gcaaatatgt aatacgcatc gacgcacaga
     9481 ccgctactgt ttacctgggc gaccacgaag atcttttcag cccggccatg ctggttgaaa
     9541 agatccatca tgtcacctcc gacgggattg taaacgggaa acggatgacg gtaaaaatcc
     9601 gttacagcgc ccccgaagcg gacgcacgca ttgagctttc cgaatcggga accacggcca
     9661 aagtgatttt tgaccagcct cagcgcgccg ttactcccgg ccaggccgcc gttttttatc
     9721 aggacggcat ggtgatggga gggggcatta tcgcccaccc gattccactc ttttaaggaa
     9781 tgccttaccc cggcagacaa cggaacgcct aatcaacgac cgccgcttca agcagcggtc
     9841 gtttcttttc attatgcggg ttttgctttt tgtttaccgg attttgcctg cccctgaatc
     9901 tccggaacag gtttcacgct gtttttggga aatttctgct tgtgattgct ctcagtcccg
     9961 tgcggctcac tcggatcggg ccttctcatc ccggcttttg ccatcgtctc accccctttt
    10021 gaatctgatc ttattttgct gagggggagc gggttttatg tatccttttt ggtcacgttg
    10081 ccttcttttc cctgcttgac aagctgattc agataaacgc ttgttcccga agtcagaatt
    10141 ccctgggtca caccgacaaa cagcgcataa agcacatccc gtaaaccggt gacagggctt
    10201 ttggaaaaca cccacagtgt agaaagaaga atactcacca cacccagtat aaagggtatc
    10261 ttccaatccg gtatttttgt tcttttaatg aaagagccca gcacattcaa caccgggata
    10321 atcaccagaa gttccggttt gatatattgc tgcaaacttt gatccattcc aaattcctcc
    10381 cgccataacg gtttttcgac ttggtgaaac aacggcctta ttcaccgctc taatcattat
    10441 atggaaaagc ccggccggtt tatgacggca ggaaaataaa aagcggacga ccggtctctt
    10501 cgaccgccgt ccgcctgact gttcaacaat taattcagat caagattcaa aagccgcgag
    10561 gcgttttcaa ataaaatctt gcggttttct tcttcggtaa gcccaagcgc gtaaaatctc
    10621 tcaagctctt cccggtgacg ggtcagcgga taatctgtcc cgaataaaac ccggtcaact
    10681 ccatgagcgc ggatcatgtt caccgatcgc tcgggcggca aagcccacag cgagctggaa
    10741 gtatcaatat acaccttttt cccgatcaaa ttcttctcgg cctcatccca ttcggaatag
    10801 ccgcccagat gagccgcgat aaccgtcagg ccgggatatt tctccagaac ccgggcaatc
    10861 cttgacggcg aggaaaattc agaatttatg tcacccgcgt gaatcaacac aggcagaact
    10921 ccctgcgcag ttctgaaaat cgggtcggtt gcagggtcat ccacaaaaaa gccctgaaaa
    10981 tcaggatgca gcttaattcc ccggagaccg agcgaccgga tacggttgat ttcaccgaca
    11041 atatcgtttt cataatgcgg atgtatggtt ccgaatccga aaatccgatc tgtcagctgc
    11101 tcgctgatcc aatcattgat attttccacc tgattcggtt tggtcgccac agcatgcacc
    11161 aacagataat cgacgccgcc ttctctggca gacgcggcaa gatccccgat tgttccgtca
    11221 cccttgcatt gaacagggta gtattggttt aaaaaatcca ccgccttttc cgccactttt
    11281 tcgggatagg cgtgggtgtg aatgtcaaag attttttgca taagagagcc tccataacaa
    11341 gtgcgttgca gagcaggacg cagaattcag actgttttag aacaaaagtc caactttaat
    11401 tttacacctg attccgaata aaagaaacct gccgggcaaa tttatctttt atttcgtccg
    11461 atttttctac cgattgtcga tttctgtatg aaaacaacga tttggatttt attttgtccc
    11521 tttcacaaac aaaaacagga gaccatagat caccggttgg tgaagcgcat agatcagcag
    11581 cgtatttctt ccaaccgcgg ccaaaaaagg gactttcatc gtgtaaaact tctccggcag
    11641 cttgttctgt cggatcggca gaccggccgc cgctccggcc aggaacagga aaatccaggg
    11701 gatcagcggg aaataatcgg ccgagtaaaa gtcggggccg ggcagaccga agagaaacga
    11761 gccgggaatg ctgtaaaaag agcccggaat taccagcatc cataaacccg gaatcccgat
    11821 tgagccggtc agaggcagaa gacaagtcag cacaaacagc aacccccata acacaagccc
    11881 cgcccggaca ggaatttttt cgatccagcg gccaaataac gcataaatca gaattgaaac
    11941 gcccataaaa tgcagaatgc caaaatagat gctctgaccc ggaatcaaca acaccgtcac
    12001 cacggtaacc gccattgcaa gggcaagtac tttcaaacct ctgagataat tgttatggga
    12061 aaatcgacag gaaacgcctg ccagaagaat aaacgctccc gcgaagggcg gctcaaaaat
    12121 ttcaagcatt gaaaagatat gcgtgttgat ctgaaaaaca tcctgcaaat caaacagcgc
    12181 atgataaaaa accatcgcca gaatagcgat gcccctgacg gcgtcgagca gatcaatccg
    12241 gctgtgcttt ttaataatca attccataat tccaattaac ctccgaatgt tccgtaacgg
    12301 gtaatcccgc tttccggcgg tgaagtgatc ggtttgtgca ttgggtttcg gatacaacat
    12361 tcgattaaaa taatcgggaa caccagtatt tctattgaaa ccgattaaaa aactacgggt
    12421 tttattgtat a
//
LOCUS       DATALY010000061         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_617960,
            whole genome shotgun sequence.
ACCESSION   DATALY010000061 DATALY010000000
VERSION     DATALY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617960"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VHR42_01860"
     CDS             complement(<1..669)
                     /locus_tag="VHR42_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3025963.1"
                     /translation="MKKEPFCTHETAWCPGCGNFTILECLKTALEQLGKDPSEVLIAA
                     GIGQAAKTPQYISANAFCGLHGRSLPAAVAAKIANERLTVIVDTGDGDSYGEGGNHFI
                     HNIRRNVDITHFVHDNQIYGLTKGQASPTSVIGLKTGVQTDGNFSEPLNPVLLAIACG
                     AGFVARAFTGHKEHLISLMKQAIEYKGYALVDILQPCVSFNKTNTFAWYNHRVYELDE
                     SYDFK"
     gene            complement(666..2336)
                     /locus_tag="VHR42_01865"
     CDS             complement(666..2336)
                     /locus_tag="VHR42_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX3025964.1"
                     /translation="MYNLLIGGAAGQGIDTTAAILEKLLKRSGYSVFTARDFMSRVRG
                     GHNFSLIRFGTKPVLSHSNRLDGIVALDQETISLHREQLKDNGFILCDDKLSESDPRL
                     IRVEMDAMAKALGNPRVSGSIAVGVILKLFGESLDNVEDVLRTFVKQPYLDINVKAVE
                     EGYQSTETRFPHLDGEYRDQMLISGSKAVALGAIAAGLRLYSAYPMSPSTAIMETLAS
                     KADEAGIVVEQAEDEIAAINMAIGASYAGARSMTGTSGGGFSLMVEALGLSGMAEIPL
                     VVIDVQRPGPVTGLPTRTEQSDLKFVISASQGEFPRMVIALRNPSDAFYQTMRAFNLA
                     EKYQIPVILLSDQYLGDATSTVGPFDPKRIQMVEPESEYSEKGEYLRYRYTETGISPR
                     LIPGKTEHLVAIDSDEHDERGWITESAEVRIKMMDKRMKKLESLKQDLQEPEFIGDDG
                     FETLLIGWGSTWGPIDEAVQLLNEDKDGRYAALVFGDVYPLPVKLLKEKAAKAHRIIN
                     VEQNATGQLADLIRQETGIICESSILKYDGRQISGEEIAERVRKGETE"
     gene            complement(2411..2785)
                     /locus_tag="VHR42_01870"
     CDS             complement(2411..2785)
                     /locus_tag="VHR42_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HEX3025965.1"
                     /translation="MSIVDFYRCERCGNIVALIKKGGGTLMCCGQAMTKLEANSTDAA
                     KEKHVPVVTKENGKIHVVVGSTLHPMLPEHSIEWIALVADDKVELKYLKPGEEPKASF
                     AEAKSGTVYVYCNLHGLWKAGF"
     gene            complement(2807..3080)
                     /locus_tag="VHR42_01875"
                     /pseudo
     CDS             complement(2807..3080)
                     /locus_tag="VHR42_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
     gene            complement(3851..4417)
                     /locus_tag="VHR42_01880"
     CDS             complement(3851..4417)
                     /locus_tag="VHR42_01880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3025966.1"
                     /translation="MNNKKIGVFVGSLRRGSFSRSIAKAAVSLAPAEFEFHFIDIGSL
                     PLYNQDFDDDGQPPESYQSFRNEVADLDGFLFVTPEYNRSIPAVLKNALDVASRPYGH
                     NVWSGKPGAIISVSPGGLGAFGANHHLRQPMVFLNVLLLQQPEVYISKVSSLLDEKGE
                     LIDESTRTYLKKFMDAFSEWVKVISESH"
BASE COUNT         1115 a         1129 c          956 g         1242 t
ORIGIN      
        1 tttaaagtca taagattcat ccagctcgta caccctatga ttgtaccacg caaaagtatt
       61 ggttttgtta aagctgacac atggttgcag gatatcgacc agcgcataac ccttgtattc
      121 gattgcctgt ttcatcagtg agatcagatg ttctttgtga cctgtaaatg cgcgcgcaac
      181 aaacccagcg ccacacgcga tggcaagaag gaccggattg agcggctcgc taaagtttcc
      241 gtccgtctga acgccggttt ttaatcctat cacggaagtc ggcgacgcct gacctttggt
      301 caatccataa atctggttgt catggacaaa atgtgtgatg tcgacattgc ggcgaatatt
      361 gtggatgaaa tggtttccac cttctccata cgagtccccg tcgccggtat cgacaattac
      421 cgtcaatctc tcatttgcaa tttttgccgc tacggccgcg ggaagggaac gcccatgaag
      481 accgcagaac gcatttgcgc tgatatactg gggcgttttc gccgcctgac cgataccggc
      541 agcaatcagc acctccgaag ggtctttgcc aagctgctca agagcggtct tgagacattc
      601 caaaatggta aaatttccgc agcccgggca ccatgccgtt tcatgcgtac aaaaaggttc
      661 ctttttcatt ctgtttcccc cttccgaacc cgctcggcaa tttcctcgcc ggaaatctgg
      721 cggccgtcat atttcaaaat actgctctcg cagataatgc cggtttcttg gcggatcaag
      781 tctgccagct gaccggtcgc gttctgctca acgttaatga tacggtgagc ttttgccgcc
      841 ttttctttga gcagcttaac aggcagaggg tagacgtcgc cgaacacaag agcagcatag
      901 cggccatcct tatcttcgtt caggagctga accgcctcat cgataggccc ccacgtggaa
      961 ccccagccaa tcaacagagt ttcgaaaccg tcgtcaccga taaattccgg ctcctgcaga
     1021 tcctgtttca ggctttccag ctttttcatg cgtttgtcca tcattttgat tcggacttcc
     1081 gccgattccg tgatccagcc gcgctcgtca tgttcatcgc tgtcgattgc cacaagatgt
     1141 tccgttttcc ccggaatcag tcggggagat atgccagttt ccgtataccg ataccgaaga
     1201 tattcgcctt tttctgaata ctccgactcc ggctcaacca tctggattct cttcggatcg
     1261 aacggtccaa cggtagacgt ggcatcacca agatactggt cactcagtag aatcacggga
     1321 atctggtatt tttccgccag attgaaagcc cgcatggtct gataaaaagc atcggacgga
     1381 ttccgcagag caatcaccat acgggggaat tcgccctgcg acgcggaaat gacaaacttc
     1441 agatcgcttt gctccgtcct tgtaggaagg ccggtcacag gccccgggcg ctggacgtca
     1501 atcactacaa gaggaatttc tgccataccg gaaaggccga gcgcttccac catcagagag
     1561 aaaccaccgc cggacgtgcc tgtcatagaa cgggcgcccg cataggaggc tccgattgcc
     1621 atatttattg cggcaatttc atcttccgcc tgctctacta caatacccgc ttcatcagct
     1681 tttgatgcaa gcgtctccat aatagccgta gagggggaca tcgggtaagc ggaatagagc
     1741 cggagaccgg cagcaatagc tccaagagca acggctttgc tgccggagat gagcatctga
     1801 tcgcgatact ccccgtcaag atgcggaaag cgcgtttctg tagactgata gccttcttct
     1861 accgctttga cattgatatc aaggtatggc tgttttacaa atgtacgcag gacatcttca
     1921 acgttatcaa gagattctcc aaacagcttc agtatgaccc ctaccgctat gctcccggag
     1981 acccgcgggt tgccgagagc cttcgccatc gcgtccattt ctacgcggat aaggcgtgga
     2041 tcggactcgg ataatttatc atcgcagaga ataaatccgt tatctttcag ctgttcccta
     2101 tgtagactga ttgtttcttg gtcaagggcg acaattccgt cgagtcgatt gctgtgcgaa
     2161 aggaccggtt tcgtcccgaa acgtatcaac gagaaattat ggcctcctcg aacccgggac
     2221 ataaaatccc ttgccgtaaa cacactgtat ccggaacgtt ttagcagttt ttcaaggatc
     2281 gccgcagtgg tgtcaattcc ctgaccggct gctcctccaa ttaataagtt atacattagg
     2341 gcctcctttc gggtttttcc tttcttgata gggagaaacc ctgcctgaac aggatctctc
     2401 atttcaccgt ttaaaaccca gctttccaaa ggccatgcaa attgcagtac acatagacgg
     2461 tacctgactt tgcttcggca aacgatgctt ttggctcttc gcccggcttt agatatttca
     2521 gttctacttt gtcgtccgca acgagtgcaa tccactcgat ggagtgctcc ggcagcatgg
     2581 gatgcagtgt agagccaacg acgacatgaa tcttaccgtt ttctttggta acgaccggaa
     2641 catgtttctc tttcgccgca tccgtcgagt tggcttccag cttcgtcatg gcctgtccgc
     2701 agcacatcaa tgtcccgccg ccttttttga tcaaggcaac gatatttccg cacctctcgc
     2761 aacgatagaa atcgacgata gacattttca agactccttt taattatttt ttattaaccg
     2821 gctgcgatag caataccggc aataatccaa atttgtcatt cccgatccat cttttcaagc
     2881 actaaattca gagtatggat tgcacgcttc agataagacg tataacaaat atcaaaatca
     2941 aattcgttct gccgaagcag ttcaccggca tcctgggctt ccttttcacc tttttcagtc
     3001 aggtcgacat ccgcccaacc ggtaaagaga ttcttttgat tccattcgct ctctccatgc
     3061 cgtaacaaaa cgagtttcac gattttgcac cctttctaca aattctacct tgattataat
     3121 cctactttac ccaaaataca acatattttt aataataata attattttca aaatagactt
     3181 caggtatcaa attgcgattc aggcatgatt ttcaggaacg ggtttcattt ttggattttt
     3241 ttatagggga aacagttcct catcattttc aaaaacggaa aagccaggga tctgcccggg
     3301 cttcctgatc aaacatcatc gcggggaatt cagccgaaca gcaacttcat tcggaatggt
     3361 ataataatta tcgccatttt tctcttttgt ccctgtgctt ttactgctga ttttttggga
     3421 attcattttt ttcattatga aagtctcccg gcattatgga acttttagtt ccgtcatgtt
     3481 ttttgttatt tcccaatcat gagatccttc cggaattcca gcaggctcat aatccaggtt
     3541 tcttttttaa tagccattct ttaccttacg cctaaagtat actaatattg tgtacttaaa
     3601 ttgatggcaa ttttgggttt ttaagcttgt tcttttcttc cttttgttta attttaaaag
     3661 aaaaaggaga ccactgaatg agtgatctcc ttgcaatagc cagcttccct cataatgtct
     3721 gttgatgcac ccagtggtac aggtgctcat aatgacaaac ttgggaaaac cataaaatga
     3781 agagttactg cgtcaagctt acaaactatt attaaaatgt ctcgattata gtcatttcga
     3841 tgcgtaagac tcaatgagac tcagagataa cttttaccca ttcagagaaa gcatccataa
     3901 actttttcag gtatgtgcgg gtcgattcgt cgataagttc tcctttttca tccaaaagcg
     3961 acgacacttt actgatatag acttccggct gttggagaag caaaacgttt agaaatacca
     4021 tgggctgacg caaatggtga ttggctccaa aggctccaag accgcctgga gatacgctga
     4081 taatggcgcc tggttttccg ctccagacgt tgtgtccata tgggcgagaa gccacatcca
     4141 gtgcattttt cagcacggcc ggtatggaac ggttgtattc cggggttaca aaaaggaaac
     4201 cgtcaagatc cgccacttca ttacgaaacg actgatagga ttcgggcggc tgcccgtcat
     4261 cgtcaaaatc ctgattatac agcggaaggc tgccaatatc gataaaatga aattcaaatt
     4321 ctgccggcgc aagcgataca gccgcctttg caatggagcg gctgaaagag cctctgcgaa
     4381 ggcttcctac aaaaacacca atctttttat tgttcataat ttattctcct tatatttttc
     4441 ag
//
LOCUS       DATALY010000062         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_791074,
            whole genome shotgun sequence.
ACCESSION   DATALY010000062 DATALY010000000
VERSION     DATALY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791074"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..909)
                     /locus_tag="VHR42_01885"
     CDS             complement(304..909)
                     /locus_tag="VHR42_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEX3025967.1"
                     /translation="MARDWKKQTSKYGSIFTDGSKTAAGTNKVLFGHSMKDGRMFADV
                     LKYANLDFYRSAPILTYQEESEVSQWKVFAVIKTNTDASQGKVFDYLKATFPSSDNFL
                     EFLYQVRLRSVLTLPVDIRPSDEILTLSTCSYEFDGFRTAVFARKIRAGESGSVQTSA
                     ACVNSKTLYPDCWYRKYGGTKPSFPSFAEARKQGAVSWLSS"
     gene            complement(920..1258)
                     /locus_tag="VHR42_01890"
     CDS             complement(920..1258)
                     /locus_tag="VHR42_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025968.1"
                     /translation="MRKRNRDENSTLRKRLITAATVLCAAVFLISAGALVYQLVILPA
                     QSDNIVNDARSLYRKDVSSSNAPSSIPSKQVKAASLAELQKINPDVQGWITVPNSVID
                     YPVLCYPKKR"
     gene            1517..2485
                     /locus_tag="VHR42_01895"
     CDS             1517..2485
                     /locus_tag="VHR42_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX3025969.1"
                     /translation="MKFSEMPYKRPDLDAMERDYSRITEEFGKADSAEEQYRLILEHE
                     KLTRDFDTMSTIAYIHNSINTADPFYEKEMAFFDANSPVMNSWRQKFYEAVDRSPLKE
                     KLEKRTGKLFFTNISLSLKSFSDATMELQKKENALVTEYEKLLASAKIEFHGETLNLS
                     EIQKHAISSDRGERSEAWTKTAGFFSENSKHLDEIFDELVRNRTEQAHLLGYENYVQL
                     GYDRLGRNCYGPKEVAEYREQIVHELVPIITDIKKHQAQRLGLPALKFWDDACIFPDG
                     NPKPQGSTKELVDAAQKMYSEMSPLTGEFFRFMRENELAGAETEQR"
BASE COUNT          656 a          632 c          634 g          607 t
ORIGIN      
        1 ggaagaaggg aatcttcaaa ggagcggtta cggagcatct ccgggtaaag gccgccgtct
       61 cccgcgcggc tgatgtcctc gaagaacagg ccgtatagat cgggtgctgt tgggaatcgt
      121 ttttgctttg tttgaatctg aattcggctc atgaaatgca cctccgagtt ttctttttaa
      181 ccatcatacc attttttccg tgagaaaata tccgaacgaa aaaataagaa aattttttcg
      241 ttcttaaaat gccaaaaagc gcggccctca aacgagagcc gcgcccgggt taaacacaca
      301 ctgtcaggaa gacagccacg aaactgctcc ctgcttcctt gcttcggcaa aggaagggaa
      361 agaaggtttt gttccgccgt attttcggta ccagcaatcc gggtagagcg ttttgctgtt
      421 gacacaggca gcggacgttt gcaccgaacc tgattctccc gcgcgtatct tgcgggcaaa
      481 cactgctgtg cggaatccgt caaattcata ggaacaggtg gaaagcgtca ggatttcgtc
      541 cgacggacga atatccaccg gcagcgtcag caccgagcgc agccgcacct ggtaaagaaa
      601 ttccagaaaa ttatccgaag aagggaacgt tgctttcagg tagtcgaaca ctttcccctg
      661 tgaagcgtct gtgttcgtct tgatgaccgc aaagaccttc cactgtgaca cctccgactc
      721 ctcctgatag gttaaaatgg gggcggaccg gtaaaagtca aggttcgcat acttcagaac
      781 atccgcaaac atccggccgt ctttcatgct gtggccaaac agaactttat tggtgccggc
      841 agcagtttta ctgccgtccg taaagatgct gccgtatttc gaagtctgct tcttccagtc
      901 acgggccaag tagaagtttt catcgttttt ttggatagca gagcacgggg taatcgatga
      961 ccgaattggg aacggtaatc cagccctgca catccgggtt tattttctgc agctccgcaa
     1021 gggaagccgc tttcacctgt tttgatggta tcgatgaggg tgcattagag gaggaaacgt
     1081 ctttccggta aagcgaacgc gcatcattta caatgttgtc tgactgcgcg ggaagaatca
     1141 ccagctggta aaccaaggct cccgcgctga ttaaaaaaac ggcggcacaa aggacggtcg
     1201 ccgcggtaat caggcgcttt cgcagtgtac tattctcatc tctgttccgt ttccgcatac
     1261 cgcaagcctc cgttttttgt tccgtgctcc gtttattgca atccgctcat gcgcattttc
     1321 cgtgtacttt ggtcagctca agaatatcat tttgcaccat gtttgtaaag ctggcagagt
     1381 aatttcggca gtgccggtat ctctctttcc tgatttccgg ggaaaacaca gcggcgtttt
     1441 ttcattttcc ggtttatccg tgaaaagtta tagtataata gttgaagaaa aagtttcctg
     1501 caaggaggtt acctcgatga aattttcaga aatgccgtac aaacgtcccg atcttgatgc
     1561 aatggaacgg gattacagcc gcattaccga ggaattcgga aaagccgatt ccgctgaaga
     1621 acaataccgg ctgattctcg aacacgaaaa gcttacccgt gacttcgaca ccatgtctac
     1681 catcgcgtac atccataaca gcatcaacac agccgacccg ttttatgaaa aggaaatggc
     1741 tttcttcgat gcgaatagcc cagtcatgaa cagctggagg cagaaatttt atgaagctgt
     1801 ggaccgttcc cctctgaaag aaaagcttga gaagcgcacc ggcaagctct ttttcaccaa
     1861 catttcactt tcgctgaaat ctttttccga tgcgaccatg gaactgcaga aaaaggaaaa
     1921 tgcactggtc acggaatacg agaagcttct cgcttccgca aaaattgagt ttcacggtga
     1981 aacgctgaac ctctcggaga ttcagaagca tgccatttcg tctgaccgtg gggaacgcag
     2041 tgaggcctgg acgaaaaccg caggtttctt ctccgaaaac tccaagcatc tcgacgagat
     2101 ttttgacgag ctggtgagaa accgcacaga gcaggctcat cttctgggct atgagaatta
     2161 tgttcagctg ggttacgacc gtctgggccg caactgctac gggccgaaag aagtggccga
     2221 ataccgtgag cagattgttc acgagcttgt gcccattatc acagacatca agaagcacca
     2281 ggcgcagcgt cttgggctcc ctgccctaaa gttctgggat gacgcctgca tcttcccgga
     2341 cggaaaccca aagccgcagg gaagcaccaa agagcttgtg gatgccgctc aaaagatgta
     2401 cagcgaaatg agcccgctga ccggtgaatt cttccgcttc atgagggaaa acgagcttgc
     2461 cggtgcggaa acggaacaga gatgagaata gtacactgcg aaagcgcctg attaccgcgg
     2521 cgaccgtcc
//
LOCUS       DATALY010000063         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_360722,
            whole genome shotgun sequence.
ACCESSION   DATALY010000063 DATALY010000000
VERSION     DATALY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360722"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..584)
                     /locus_tag="VHR42_01900"
     CDS             complement(150..584)
                     /locus_tag="VHR42_01900"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX3025970.1"
                     /translation="MKTIGIIGGMSWESTATYYLELNRYINKKMGGYYSAKCILYNVQ
                     YQEIKEIHKSGDWERAGEILGEAALTLQNAGADFIILATNTMHLVASRIQEKIHIPFL
                     HIAEVTADKLLEDGIKSVALLFQSGYDIAEIAAGRVLDFPDD"
     gene            complement(604..1029)
                     /locus_tag="VHR42_01905"
     CDS             complement(604..1029)
                     /locus_tag="VHR42_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025971.1"
                     /translation="MLGAVFLLLGIMSRAGILKTDPASRGDPGIVFPILGSAFLIVGI
                     SSALAASIKGKRKAQLFESGMPVTAKIDAIKQNFFTKWGSSHPYIIYFTYEVDGVPYK
                     GKSGLFWVLPSVREHDHETVFVDMDHPKRYALKLTSEDS"
     gene            1414..3327
                     /gene="cooS"
                     /locus_tag="VHR42_01910"
     CDS             1414..3327
                     /gene="cooS"
                     /locus_tag="VHR42_01910"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HEX3025972.1"
                     /translation="MDEAIREKTQGRVSYHDSVEEMLQKIRKDGLSNAFDRYALQEKI
                     RCKFCLEGLSCQLCSDGPCRISEKGGQEKGVCGIGPDAMAMRTLLLHNIMGAGTYSHH
                     AYEAFRTLRETARGKTCFQIKDPAKLKWMCGKVGINTSQSDNLMAEQLADLLNDEMSK
                     GPDCPSVMVEAFAPKKRKDVWRKLNIYPSGVVHEEQNCVASCLTNVDGDYVSLAKKAL
                     RLGLATIYTAQIGLEMVQDILYGTPTPHEVNTDLGIMDADYVNIVFNGHQPWVGVATL
                     QKAKLPEIQQKAKDAGAKGLRIVGSIETGQELLQRFPVDDVFTGLMGDWLTIEPLLAT
                     GTVDVFAMDENCSPPAMDQYAEKYQAALVSISSIIGVPGLSQFLPYHPQDADKMADSL
                     ITLAIENFQKRHGTITPTVPKKIQKAIAGFSTEAVLSALGNQLDPLVEVIKAGKIKGV
                     VALASCSTLRNGPQDWNTVNLTKELIKRDLLVVSCGCGNHALEVAGLCDLCAKDLAGP
                     GLNEVCGMLKIPPVLSFGTCTDTGRVSMLVTALADFLDVDVPQLPIAVTAPEWMEQKA
                     TIDGVFAVAYGAYTHLSPTPFITGAPQLVELLTQDIESLTGGKVALGDDPVQVANDME
                     AHINLKRKALGLN"
     gene            complement(3381..4094)
                     /locus_tag="VHR42_01915"
     CDS             complement(3381..4094)
                     /locus_tag="VHR42_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025973.1"
                     /translation="MKNCKLTLLCLSLLLVTAAGCSMQNGETAGSSVISSSEINRPSV
                     SSQVPSSSSQPPVLSSSSVPADSAGSQPASGNASSGSKVVKSASSAAKVVSQAESKSS
                     QVSPTWVAYIDNDHHLHVKKKDGSNDKILVKDVEEAPCVAGDWVYFLPDLNEIDKVKL
                     DGSQRTKVCSTDPFIVYNANLNISHEINGSSSVTAEYKDGYILYTCFQLKEVGEEKTN
                     PPSYYKLDLAQNKLIPVKK"
     gene            complement(4138..4893)
                     /locus_tag="VHR42_01920"
     CDS             complement(4138..4893)
                     /locus_tag="VHR42_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062192.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX3025974.1"
                     /translation="MIFYFSGTGNSFHVASEIAKTQGELPVSIAKELDQGKLEYQLKQ
                     GERIGFVYPIYAWAPPKIVLDFIKKMKISCEKPYVFSISTCAGNEGNATPLLQKALST
                     KGLTLDSAFSVLMPSNYVTGADICSKDEAAKIVKRAEETIKTINDIIEKKQTGVFQLI
                     PGGKPFLHTSIINPLFNSFGRSTKNFYATDACTHCGLCEKLCPVHTITVKEKPVWGKR
                     CTQCLACINRCPAKAIQYGAHTAERGRYVFPNE"
     gene            complement(4940..5632)
                     /locus_tag="VHR42_01925"
     CDS             complement(4940..5632)
                     /locus_tag="VHR42_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018882925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEX3025975.1"
                     /translation="MRVKNFQNDLYTGKIVRYNLTSVKIKRGVRNVTDKPYHHGNLKN
                     ELIEAGISLINEEGLSGFSLRKVATKCNVSNAAPYSHFNSVDDLIAAMGQHVTEHFME
                     KLRASIRGQEENPQATKLLGIAYIDFFAENPQYFQFLFYHSGLKINMDQESPDDYPPF
                     ALFRTTAYQLFRSQSIPEENYKEKLAALWSSVHGIASLLSSQGIQYSGSWHEVLAQLT
                     TRKEKPNPDSAS"
BASE COUNT         1490 a         1337 c         1452 g         1488 t
ORIGIN      
        1 aagacggtat caaatcggta gctctgctgg gtacccgctt taccatggaa aaagattttt
       61 ataaaaatat actgatcagc cgcggaatcg atgttcagat tcccctaaag gaagatatcg
      121 accggattca caaaattatc aatcaggaat taatcatcgg ggaaatcaag gactcttccc
      181 gcagctattt cagcaatgtc ataaccggat tgaaacagca gggctaccga tttgataccg
      241 tcttccagca gcttatccgc tgttacttcg gcaatatgca ggaagggaat atgaatcttt
      301 tcctgtatcc ttgaggctac aagatgcatg gtgtttgtgg cgagaataat aaaatcggcg
      361 ccggcattct gaagagtcaa tgctgcttcg ccgagaatct cgccggcacg ttcccaatca
      421 ccgcttttat gaatctcctt gatctcctga tactgaacgt tatataaaat gcatttcgcg
      481 ctgtaatatc cgcccatctt tttattgatg tatcggttta gctccaaata gtaagttgcg
      541 gttgattccc agctcatccc gcctatgata ccgatggttt tcatgtgcac tctcctttat
      601 gattcacgaa tcctcgctgg ttagcttcaa tgcataacgc ttcggatggt ccatatcgac
      661 aaaaaccgtt tcgtgatcgt gttctctgac ggacgggaga acccagaaaa ggccgctctt
      721 ccctttgtag ggaacgccat ccacttcata ggtgaaatag atgatatacg gatgggaaga
      781 accccacttg gtaaaaaagt tctgctttat ggcatcgatt tttgctgtta caggcattcc
      841 ggattcaaag agttgtgctt ttctttttcc ttttatgctt gccgcaagcg cggaagagat
      901 tcctacgatc aggaaggcac tgcccagaat tggaaataca atccccggat ctccacggga
      961 agcgggatcg gtttttaaaa tgcctgctct tgacataatt cccaaaagca aaaataccgc
     1021 tcctaacaaa atgaaaagct ccccgaagaa tccccacatg ctttttggct ttctcgtaaa
     1081 aacaccccct gaacctgaat gataggacag ccggattttt cgtccttttt tccatcatac
     1141 actgaatttc tttccgatgc aatcttaaga aatgggaaag gggtttgcgg aatgaaccta
     1201 cattcggaag tcgcctctgt cgcggcccaa atcgaatgca ggctgcctca tcctggaccc
     1261 accgatgaat gttctggagt atttcaagtg aaagacgctt ttgtttcttt ttctcattcg
     1321 acgttccgaa actatcgcag ccgtttatat gaatcttggt taattcttca taaatcggtg
     1381 aaaatagcat catcaaaaac aggaggttta agaatggatg aagctattcg tgaaaaaacg
     1441 caggggcggg taagttacca tgattccgtg gaggaaatgc tgcaaaagat ccgaaaggac
     1501 gggctttcca acgcttttga ccgctatgcg ctgcaggaaa agatccgatg caaattctgt
     1561 ctggagggcc tgagctgtca gctttgctcc gacggaccct gccggatcag tgaaaaaggc
     1621 ggtcaggaga agggcgtctg cggcattggc cccgacgcaa tggcaatgag aacgctgctg
     1681 ctccataata ttatgggtgc cggaacttac agccatcatg cctacgaagc attccggacg
     1741 cttcgagaga ccgcgcgcgg gaaaacctgt ttccagatca aagaccccgc caaactgaaa
     1801 tggatgtgcg ggaaggtcgg aatcaacacc agtcaaagcg acaatctaat ggcggagcag
     1861 cttgccgacc ttttgaatga cgaaatgtcg aaaggcccgg actgtcccag tgtgatggta
     1921 gaagcatttg ctccgaaaaa acgcaaagat gtctggcgta agctcaatat ctatccgtcg
     1981 ggagttgtgc acgaagagca gaactgcgtt gccagctgcc tgacgaacgt cgacggcgat
     2041 tacgtatcgc ttgccaaaaa ggcgctgcgg ttagggcttg caacaattta caccgctcag
     2101 atcgggcttg aaatggtaca ggatatctta tacggaacgc cgacaccgca tgaagtaaat
     2161 actgacctgg gaatcatgga cgccgactac gtcaatattg tctttaacgg gcaccagccg
     2221 tgggtggggg ttgcaacgct gcaaaaggca aagctgccgg aaattcagca gaaagccaaa
     2281 gacgcgggag ccaagggcct gcggatcgtc ggctccattg aaacggggca agagctgctg
     2341 caaaggtttc ccgtggacga tgtctttacc ggtctgatgg gcgattggtt aaccattgag
     2401 ccgctgctgg cgaccggcac ggtggacgtt tttgccatgg atgagaactg ttctccgccg
     2461 gctatggacc agtatgcgga aaaatatcag gccgcgctcg tcagcattag ctcgatcatc
     2521 ggggttcccg gactcagtca gttcctgccg tatcatccgc aggacgcgga taaaatggcg
     2581 gacagcctga ttactctggc aattgagaac tttcagaaac ggcacggcac aattacgcct
     2641 accgttccga aaaaaataca aaaagcgatc gcagggtttt cgaccgaggc ggtgttgagc
     2701 gcactgggaa accaacttga tcctctggtc gaagtcatta aggcaggcaa aattaagggc
     2761 gttgtcgccc ttgcaagctg ttcgacgctg aggaatggcc cgcaggattg gaatacggtt
     2821 aatctgacga aggaactgat caaaagggac ctgctggttg tcagctgcgg atgcggaaac
     2881 cacgcgcttg aggtggcagg tctctgtgac ctttgcgcga aggatcttgc ggggccgggt
     2941 ttgaatgagg tttgcggcat gctgaaaatt ccccctgtat tgagctttgg cacctgtacc
     3001 gataccggca gagtttcgat gcttgtcacc gcgctggccg attttctgga tgttgacgtg
     3061 cctcagctgc cgattgccgt cacggctccg gaatggatgg aacaaaaagc aacgatcgac
     3121 ggcgtttttg cagttgcata cggggcatac acacatctgt ctcccacgcc gtttatcacc
     3181 ggggcgccgc aattagttga gctgctgact caggacattg aatcccttac aggaggaaaa
     3241 gttgctttgg gggatgatcc ggttcaggtg gcaaacgata tggaagcgca tattaatctg
     3301 aaacgaaaag cgttgggact taattaagaa accgagtcaa aagatgctcc gcccaaaaca
     3361 ggacggagca tcttttgcgc ttacttcttc acggggatca gtttattctg tgcaaggtcc
     3421 aatttataat aggaaggagg attcgttttt tcctctccta cttctttcag ctgaaagcag
     3481 gtatataaaa tataaccatc cttatactcg gcggtgacgg aagagctgcc gttaatctca
     3541 tgactgatat ttaaattcgc attatatacg ataaagggat cggtactgca cacttttgtc
     3601 cgttgcgaac catccagttt tactttgtcg atttcattca aatccggaag gaaataaacc
     3661 caatcaccgg ctacgcaggg agcttcctca acgtctttca cgagtatctt gtcattggaa
     3721 ccgtcttttt tcttaacgtg gaggtgatga tcattatcga tataagctac ccacgtggga
     3781 ctgacctgcg aacttttgct ttccgcttgg gaaacgactt tcgcagcaga agaagcgctt
     3841 ttcacaactt tggaaccgga gcttgcgtta ccggatgctg gttgagaacc tgcagaatca
     3901 gcaggaaccg aggatgacga aagtaccggc ggctgggacg atgacgaggg cacctgagaa
     3961 gaaaccgaag gacggttgat ctccgacgag ctgatcacgg aacttcctgc agtttctccg
     4021 ttttgcatgc tgcatcccgc cgcggtgacg agaagaagtg ataagcaaag aagagttaat
     4081 ttgcaattct tcatataaaa gaccctccat ttggaagcta ttcaccgctg gcttcgctta
     4141 ttcattcggg aagacataac ggccccgttc cgcggtatgg gccccatatt ggattgcctt
     4201 tgcaggacag cggttaatgc aggctaagca ttgcgtacag cgtttgcccc atacgggctt
     4261 ttcttttacg gtaatggtat gaacggggca tagcttttca caaaggccgc agtgggtgca
     4321 ggcatcggtt gcgtagaaat tttttgtgct tctgccaaag gagttaaaga gaggattaat
     4381 aatggaagta tgaaggaacg gtttaccgcc ggggatcagt tggaaaacgc cggtctgctt
     4441 tttttcaatg atgtcattga ttgtcttgat cgtttcttcc gcacgtttca caatctttgc
     4501 ggcttcatct ttggagcaga tatctgctcc tgtgacgtaa ttgctcggca tgagaacgga
     4561 aaaagcgctg tcaagagtca aacccttcgt gctgagcgct ttttggagaa ggggcgtggc
     4621 gtttccttca ttgcccgcgc aggtgctgat ggagaacacg tacggtttct cacagctgat
     4681 tttcattttt ttgatgaaat caaggacgat tttgggaggc gcccatgcgt agatcggata
     4741 tacaaatccg attcgttcgc cctgctttaa ttggtattcg agctttccct gatccaattc
     4801 ttttgcaatg gacacgggga gctcgccctg tgtcttcgca atttctgaag caacgtggaa
     4861 cgagtttccc gtccctgaga aataaaaaat catttgtctc tgctccttta aaatcgattt
     4921 gttaactcac actttccagt caggaggcgc tgtccggatt cggtttttcc tttcgggtgg
     4981 tgagctgagc caatacttcg tgccagctgc cggaatattg aattccctgt gaggagagca
     5041 gcgaggcgat gccgtgcacc gatgaccaca gcgcggccag tttttcctta tagttttctt
     5101 cgggaatact ctgactgcga aaaagctgat aggccgtggt gcggaacagg gcgaacggag
     5161 ggtaatcatc cggactttcc tgatccatat ttattttcag gccgctatga tagaagagaa
     5221 attgaaagta ctgcggattt tccgcaaaaa aatcgatgta ggcgatgcca agaagcttgg
     5281 ttgcctgggg attttcttcc tgaccacgga tggacgcgcg aagcttctcc ataaaatgct
     5341 ccgtaacatg ctgccccatt gcggcgatca gatcgtctac actgttgaaa tggctgtagg
     5401 gtgcggcgtt gctgacgtta cattttgtgg caactttcct gagagaaaac ccgctcagcc
     5461 cttcttcatt aatgagcgaa ataccggctt caatcaattc gttttttaaa ttaccatggt
     5521 ggtaaggctt atcggtcacg ttgcggacac ctcttttaat ctttacactg gtaagattat
     5581 aacgtacaat cttaccagtg taaagatcgt tttggaaatt ttttaccctc aagcacatcg
     5641 attccgaaaa ctattatgac tatatgaaga aataaaaagc ccttgataca ataaaatgcg
     5701 gtccgacggc tgcaaaataa agtatcaagg gacatgacaa accaacagaa tgctggggaa
     5761 gcggcaa
//
LOCUS       DATALY010000064        10908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_705277,
            whole genome shotgun sequence.
ACCESSION   DATALY010000064 DATALY010000000
VERSION     DATALY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705277"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VHR42_01930"
     CDS             complement(<1..769)
                     /locus_tag="VHR42_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX3025976.1"
                     /translation="MDIFNFFGSVLGYLLWFFYLIVHNYGIAIILFTISLKIITFPFS
                     VKSQKSMAMNSRMMLKQQELQKKYGNDKAKYQEELQKLYEKEGINPASGCLTNFVPLI
                     VMMGLYYTVINPLANALHVSGDAVTKAVNMLKMVPGLGSTFNTNYAQIEIIKNFQSLK
                     PFLTMFSGDEMARMESFSHGFQFLGLNLLDSPADGANIFGTLFRSNLWLIPVLCLVTS
                     LITQFFMNKMQPGMQQQQGCMKGMLYVLPLFSAWIACS"
     gene            complement(794..1021)
                     /gene="yidD"
                     /locus_tag="VHR42_01935"
     CDS             complement(794..1021)
                     /gene="yidD"
                     /locus_tag="VHR42_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX3025977.1"
                     /translation="MKRLLLGLIRFYQAAVSPYKQFHCKYYPTCSNYAIQAIERFGAL
                     KGTCLAVYRILRCNPFSRGGYDPVPEKREKR"
     gene            complement(1018..1353)
                     /gene="rnpA"
                     /locus_tag="VHR42_01940"
     CDS             complement(1018..1353)
                     /gene="rnpA"
                     /locus_tag="VHR42_01940"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3025978.1"
                     /translation="MNSYLPIKENKDFRRIYNRGKSYVSPVLVTYVLKNRNNTVRIGI
                     TTSKKTGNAVKRSRARRVIREAFRTMERSVKPGYDFIFVARGKTPFVKSTDVCRAMTA
                     QLKNAGVLE"
     gene            complement(1437..1571)
                     /gene="rpmH"
                     /locus_tag="VHR42_01945"
     CDS             complement(1437..1571)
                     /gene="rpmH"
                     /locus_tag="VHR42_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX3025979.1"
                     /translation="MLRTYQPKKLHRQKEHGFRKRMSTSNGRKVLARRRAKGRARLSY
                     "
     gene            1932..3248
                     /gene="dnaA"
                     /locus_tag="VHR42_01950"
     CDS             1932..3248
                     /gene="dnaA"
                     /locus_tag="VHR42_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX3025980.1"
                     /translation="MESFTEVWNLVCDYCKNKITEIAYSTWISRIEPVSLDFAQGTAV
                     LKVPNELHRQTLTHYYKDLLDEAFRQIFGQEIQIRICTPDEAETEKQNGDETPPINDD
                     YEFTFDTYIVGPSNKFAHAASMAVANKPATLYNPLFIYGNSGLGKTHLLYAICNEIGK
                     NHPEMNLIYIKGDEFTNELIDAIRRGTTAEFHNRYRKADVLLVDDIQFIAGKDSTQEE
                     FFHTFNTLYEAKKQIVLTSDRPPKDIATLEERLLTRFEWGLTADIQPPDFETRIAIIK
                     RKAELLEIDLPDNVAEYIANRLKNNIRQLEGAVKKMKAYHLLAGEGLSIATAQAAISD
                     IINNDQPAPFTVEKIIEEVARTFGTTGDDIRSSKRSANISSARQVAIYVVREITQMPM
                     TAIGEEFGGRDHSTVVYAIQQVEKGIARDPKTKATVEDIIKNIRDR"
     gene            3620..4726
                     /gene="dnaN"
                     /locus_tag="VHR42_01955"
     CDS             3620..4726
                     /gene="dnaN"
                     /locus_tag="VHR42_01955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX3025981.1"
                     /translation="MKISCSRQQLNEAVLNIQRAVSSKSSVPALEGILLKASQDELSL
                     CGYDLELGMTTKMEARVEESGSVVLSARLFGDIVRRLPADSVNLSVDAKNITSIKSGP
                     AEFSIVGIPADEYPELPSVTGETSIKIAHNVLKSMIRQTLFAVAESDAKPIHTGTLFE
                     LEQNRIRLVSVDGYRLALREEPAQCGEQMSFVVPGKTLGEVLKLLEDDDSELEILIGR
                     RHIIFQIGTYCVISRLLEGEFLDYKAAIPAASSTEIIVPTRSFIDSVERVSLLITDRL
                     KSPVRCRFEENEIKVSCSTSIGRANDQLEANITGSPVEMGFNNRYLLDALRNTEGDEV
                     RVQLNGALSPMKILPREGDAFLFLVLPVRLKSDS"
     gene            4774..4992
                     /locus_tag="VHR42_01960"
     CDS             4774..4992
                     /locus_tag="VHR42_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX3025982.1"
                     /translation="MTEKKIIIETKYIRLDALLKLAGAFETGGQAKVAIQGGEVKVNG
                     EVCTMRGKKMRPGDRAEFSGTVYEVCDG"
     gene            4989..6056
                     /gene="recF"
                     /locus_tag="VHR42_01965"
     CDS             4989..6056
                     /gene="recF"
                     /locus_tag="VHR42_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX3025983.1"
                     /translation="MKILRLDYSGYRNLQPGSIEPDGGINVIYGKNAQGKTNLLEAMW
                     LFTGGRSFRGSRDADLIGAGERQARLDLHFFSEEREQAARILIENGKRSATLNEVPKR
                     SAAGLIGSFCAVIFSPEHLSLVREGPAFRRNFIDSALCQIQPSYAALLTRYNRTLIQR
                     NSLLKDIPRHAQLLDTLEIWDEKLVRCGMEVVRQRIEYIRQIGEPAEAVYAGICEKKE
                     SLGLNYLKSAENLKQALYAARVSDIAGGHTSVGPHRDDLEIQIDGRSARAFGSQGQKR
                     SAVLALKLAEAEILRRRTGEKPVVLLDDVMSELDSGRQDYLLNHLENFQVFLTCCEPD
                     AVKRLKTGRLFEMSSGRLEQK"
     gene            6076..6348
                     /locus_tag="VHR42_01970"
     CDS             6076..6348
                     /locus_tag="VHR42_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HEX3025984.1"
                     /translation="MYLHLGQDTVIKIQDIIGIFDMETSTISKVTRNYLADAQKAGRV
                     VNVSMEMPKSFVLCCKKSGEITVYITQISSSTLLKRTGYMEDISNV"
     gene            6363..6794
                     /locus_tag="VHR42_01975"
     CDS             6363..6794
                     /locus_tag="VHR42_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025985.1"
                     /translation="MKLSKVQCPYCGKKLGLTAAWILRTQGEYHCPDCGAYSNIEMSG
                     GIYLLAVIAAILSAGAFLVQLVLVRSFSWLSLAVVCIPFILFFLASPFFVRLQKPAVR
                     RRRPSPPQASDTQNFTPPNASPGNPADSDRLERTIVMRRLK"
     gene            6808..7137
                     /locus_tag="VHR42_01980"
     CDS             6808..7137
                     /locus_tag="VHR42_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025986.1"
                     /translation="MLKLPLCPYCGARFLYPTVKESIKYKTKSCPHCNNTYKINSKYK
                     YFLFLAAILLLVGVNWLFLTIPSMNLLFLTGITALGVIAAYFLIPYTIRYQKTGPAAE
                     HPKKSRK"
     gene            7183..9123
                     /gene="gyrB"
                     /locus_tag="VHR42_01985"
     CDS             7183..9123
                     /gene="gyrB"
                     /locus_tag="VHR42_01985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX3025987.1"
                     /translation="MEFSEITQTQQKYDANQIQVLEGLEAVRKRPGMYIGSTGPRGLH
                     HLVYEIVDNAIDEALAGFCDKIDVKILPGDVISVRDNGRGIPVGIQPKLGIPAVTVVY
                     TVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVEVYVDGKIYCQRFERGKAVNE
                     LKQCGDTEQTGTLVRFKPDSEIFQETTAFDYETLQTRLREQAFLNAGIHIELVDERDP
                     QNRIQNNYCYHGGISSFVEFINKKKAVETVHPDIIHFSAVAPDDNATAEVAMQYNDSY
                     NELMLSFANNIHTTDGGTHEDGFKRALTRVMNDYARKYNILKENDKNLSGEDVREGLT
                     VIISVKLKEAQFEGQTKARLGNTEIATLVGNMISEKLSTYLEENPATARAIFDKALAA
                     SRARDAARKARDLVRRKSALENAALPGKLADCQSRNPDETEIYIVEGDSAGGSAKSGR
                     NRKYQAILPLWGKMLNVEKARLDKVYGNEKLMPVVTALGCGIGEEFDLSKLRYGKIII
                     MADADVDGSHIRTLLLTFFFRFMKPLVEEGHVYLAQPPLFRLTKAKNHYYAYTDPERD
                     RLMTELGSNYEIQRYKGLGEMDAEQLWETTMNPETRTMLRVEVEDAAAADEAFTILMG
                     DKVEPRKEFIEKNAKYVTNLDI"
     gene            9174..9902
                     /locus_tag="VHR42_01990"
     CDS             9174..9902
                     /locus_tag="VHR42_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025988.1"
                     /translation="MKKFISEQDSAAKPPEPQGERTPAEADESLPEYESGEELLDETM
                     EELQENEDTEEPIEWDGSEAELVADEDAAEYEEQNTGIQLKYVLKSQEIFRVLFKTQM
                     TPKRTALTVTAALLGVALGVYFLIRTVNGDHIAGSPGAICLILAGIILASPVLHIRSQ
                     AKNSENGKEIRMKIYPDHIQMGRGEGGWEIPLDGTVVRNVLKGLIFFYIDGNRLVILP
                     LRCVEPSVLPEVQAMIFAGTSPSR"
     gene            9953..10531
                     /locus_tag="VHR42_01995"
     CDS             9953..10531
                     /locus_tag="VHR42_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX3025989.1"
                     /translation="MPTFYEGNPIETVEQIISEQDYADAYYAAHSSSALLHRRDVQAG
                     ICLSLAVLCASVIPLYRARFSTFWGPVCGILAFLALAAVFFFLKPQDTKNWARRLYRS
                     NALMALPQKVTVYRDSIVLENGRERFSEYWTDFSGCVETKDAFILTGGRERLLLLIKK
                     KGLSPERQNMLSAHFADAFAYRFRKFGRQGGT"
     gene            10534..10854
                     /locus_tag="VHR42_02000"
     CDS             10534..10854
                     /locus_tag="VHR42_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3025990.1"
                     /translation="MKNPISVSFLVTEPDFVDFKTAAAKACVKKSEIYLLRVTGCVLI
                     FTALVLCAFYSGPFGRNALYILIAAAGAVLGAFHDLTIHCLVRRQAASFYEENKEKFG
                     AQNT"
BASE COUNT         2747 a         2832 c         2872 g         2457 t
ORIGIN      
        1 tggaacacgc gatccaggcg ctgaacagcg gaagaacata aagcatgccc ttcatgcatc
       61 cctgctgctg ctgcatgccg ggctgcattt tgttcataaa gaattgcgta atcagggatg
      121 taaccaggca cagtaccggg attagccaaa ggttggaacg gaacagcgtt ccgaaaatat
      181 tggcgccgtc cgccggagaa tcgagcagat tcaggccaag gaactgaaag ccgtggctga
      241 aggattccat gcgggccatt tcgtcgccgc tgaacatggt caaaaacggc ttcaggcttt
      301 gaaagttctt tataatttct atctgcgcgt aattggtgtt aaaagtggag cccagcccgg
      361 gcaccatttt cagcatattc accgccttgg tgaccgcgtc gccggaaaca tgcagcgcgt
      421 tggccagcgg gttgattacg gtataataca gacccatcat gacaatcagc ggcacaaagt
      481 tggtcaggca gccgcttgcc gggttgattc cttctttttc ataaagcttt tgaagctctt
      541 cctgatattt cgccttgtcg tttccgtatt ttttctgcag ctcctgctgc ttgagcatca
      601 tccggctgtt catcgccatg gacttttgcg acttgacgga aaacgggaac gttatgattt
      661 tcagcgaaat tgtaaacagg ataatcgcaa ttccgtaatt atggacgatc agataaaaaa
      721 accagagcag ataaccgaga acgcttccga aaaaattaaa aatatccata caggtaaatt
      781 ctcctctatt tggttatcgc ttttcccttt tttcagggac cgggtcatac ccgccccggc
      841 taaacgggtt gcagcgcaat attcggtata cagcgaggca cgtccctttc agcgccccga
      901 atcgttcaat cgcctgtatc gcataattcg aacaggtcgg ataatatttg cagtgaaact
      961 gcttgtacgg ggaaacggcc gcctgataaa aacggatcag cccgagcagc aggcgtttca
     1021 ttccagaacc cccgcgtttt tcaactgcgc cgtcatggcg cggcaaacat ccgtgctttt
     1081 cacaaacggg gtttttcccc ttgcgacaaa aataaaatcg taaccgggct ttacgctccg
     1141 ctccattgtg cggaacgcct cgcgaatcac gcggcgcgcg cggcttcgct ttacggcatt
     1201 gccggttttt ttactggtcg taatgccgat tcgcacggtg ttgtttcgat ttttcagcac
     1261 gtaagtcacc agaacggggc ttacatagga cttccccctg ttataaattc tacgaaaatc
     1321 tttattttct ttaatcggca aatagctgtt catagattga aaccgcacca aaaatccttt
     1381 tcaaatacgc aagaccgaaa gctaaaaaga aaggccactg gaagtggccc ttttgcttag
     1441 tagctcaagc gtgctctgcc cttagccctt ctgcgagcca ataccttgcg gccgttgctt
     1501 gtggacattc tttttctgaa accgtgctcc ttttgtctgt ggagcttttt gggctgatat
     1561 gttctcagca tgaaaaaatc ctcctttcgg ggtacaaacc ttgcaatata gcattatata
     1621 ttatattctt gcagcctgtc aaccataatt tcaccgaaca ggctaaaaaa aaggcttatc
     1681 tgcctgtaat ttggaaaacc ttttgccatt gttgttcttt tgattaaata taccacaata
     1741 tgaggcaaaa taaaaggaaa agagattaaa actgaaaaaa atttatcttt tggtgtttct
     1801 taaaatcttt cctacaatat aatgtataac aggaaagaaa tgaaatttgt caaacctggc
     1861 attttgtgat aatatattct ttagttatgc ctgaaaatat aaatattagg aaatcaaatg
     1921 aggttttaaa aatggaatcc ttcaccgaag tctggaattt agtctgcgat tactgcaaaa
     1981 acaaaatcac cgagattgcg tattctacct ggatcagcag gattgaaccc gtgagccttg
     2041 actttgcgca gggaaccgcg gtactgaagg tgccgaacga actgcaccgt cagacgctga
     2101 ctcattatta taaggatctg ctggatgaag cgttccgaca gattttcggt caggagatac
     2161 agatccgcat ttgcaccccg gacgaagcgg aaacggaaaa gcagaacggc gacgaaacac
     2221 ccccgatcaa cgacgactat gaattcacct ttgacactta tattgtcggg ccgtccaaca
     2281 aatttgccca cgcggcttcc atggcggtgg cgaataaacc ggctacgcta tataatcccc
     2341 tgtttatata tggaaattca gggcttggaa aaactcacct tctttatgcc atctgtaatg
     2401 aaatcggaaa aaaccaccct gaaatgaacc ttatctatat taaaggcgac gagtttacga
     2461 acgaactgat cgacgccatc cgccgcggca ccaccgcgga atttcataac cggtaccgaa
     2521 aggcggacgt gcttctggtc gacgatatcc agttcatcgc cggcaaggat tcgacacagg
     2581 aagaattctt tcacaccttc aatacgcttt acgaggcgaa aaaacagatt gtgctgactt
     2641 ccgaccggcc gcccaaggat atcgccacgc tggaggagcg ccttctgacc cggttcgaat
     2701 ggggcctgac cgcggacatc caacccccgg attttgaaac ccgtatcgcc attatcaaac
     2761 ggaaagcgga gcttctggaa atcgacctgc ccgacaatgt ggcggaatac atagccaacc
     2821 ggctgaaaaa caacatccgt cagctggagg gcgcggtaaa aaaaatgaaa gcataccacc
     2881 tgctggccgg cgagggcctg tccatcgcga ccgcccaggc ggccatcagc gatatcatca
     2941 acaatgacca gcccgcaccg ttcaccgtcg aaaaaattat cgaagaggtt gcccggactt
     3001 tcgggacaac cggcgacgat atccgttcct ccaagcgttc ggcgaatatt tcaagcgccc
     3061 ggcaggttgc catctacgtc gtaagggaaa ttacccagat gccgatgacc gccatcggcg
     3121 aggaattcgg cgggcgcgac cactccacgg tggtttacgc cattcagcag gtcgaaaagg
     3181 gaattgcccg cgaccctaag actaaggcga ccgtcgagga tatcatcaag aatatccgcg
     3241 accggtagaa cgggattctt ttccccttct ccgcgaaaag gcgttgtttt ccgcacgccc
     3301 tgcgggaaag gcggaaaagt tcaaaaactt tcgacttttt cacaaaaccg gttaaatacc
     3361 gattttcggc gctgaaagtt ttccccgaag tttttcactt tttttcaaca ttccccacag
     3421 agttttcaac agtatgttaa atactaaaaa tttaatacgg aatgttaaca accgtttcac
     3481 aacgcgttct gaaaaataaa cctgagattt ccgcatgacc gctggaaaaa cggaacattt
     3541 caacttttcc gcgcccccta ctgctcttac taaattaaat cttttattgc tattataaaa
     3601 ggatgcgaaa ggtgagctta tgaaaatttc atgcagccgg cagcaattaa atgaagccgt
     3661 gttgaatatt caaagggccg tatcgtccaa gtcctctgtt ccggccctgg agggaatcct
     3721 tctgaaagcg tcacaggatg agctttcgct ctgcggctac gacctggagt taggcatgac
     3781 caccaagatg gaagcccgtg tggaagaatc cggcagcgtc gttttaagcg cgcgcctgtt
     3841 tggggacatt gtccgcagac tgccggcgga ttccgtgaat ctttcggtgg acgcgaaaaa
     3901 cattacttcc atcaaaagcg gaccggcgga attctccatt gtgggaatcc cggcggacga
     3961 ataccccgag ctgccctccg tcaccgggga aacttccatt aaaatcgcgc acaacgtttt
     4021 gaaaagcatg atccgccaga cgctgttcgc ggtggcggaa agcgacgcaa agccgattca
     4081 caccggaacc ctgtttgaac tggagcagaa tcgcatccgg ctcgtttcgg tggacggcta
     4141 ccggctggct ctgcgggagg aaccggcaca gtgcggcgag cagatgagct ttgtcgtgcc
     4201 gggcaaaacg ctgggcgaag tattgaaact gctggaggac gacgacagcg agcttgaaat
     4261 actgatcggc aggcgccaca ttatcttcca gatcggaacc tactgtgtga tttcccgtct
     4321 gctggaaggg gaattcctcg attacaaggc ggcgattccc gcggcgagtt cgacggaaat
     4381 catcgttccc acccgctcgt tcatcgacag tgtggagcgc gtttcactcc tgattaccga
     4441 ccggctgaaa agcccggttc gctgccgctt cgaagaaaat gaaatcaagg tttcctgttc
     4501 cacctccatc ggccgggcaa acgaccagct ggaggcgaac attaccggca gtccggtgga
     4561 aatgggcttt aacaaccgtt atctgctcga cgcgctgcgc aataccgagg gcgacgaagt
     4621 gcgggttcag cttaacgggg cgctcagccc gatgaaaatt cttccccgcg agggcgacgc
     4681 cttcctgttc ctggtgctgc cggttcgcct gaagagcgat tcctgattga attcacagga
     4741 aagtaacagt tcgttacaga aaaggataaa aaaatgaccg agaaaaagat tataatcgaa
     4801 acgaagtata tccggctgga cgcacttttg aagcttgccg gcgcttttga aaccggcggt
     4861 caggcaaaag tcgcgattca gggcggcgaa gtgaaggtga acggagaagt ctgcaccatg
     4921 cggggcaaaa aaatgcgccc cggcgaccgc gctgaattca gcggaaccgt ttatgaggtg
     4981 tgtgacgggt gaaaatcctg cgccttgatt attccggata ccgcaacctt cagcccggaa
     5041 gtatcgaacc ggacggcggg atcaatgtaa tatacggcaa aaacgcccag gggaaaacca
     5101 atctgctgga agccatgtgg ctttttaccg gcgggcgctc gttccgaggt tccagagacg
     5161 ccgacctgat cggcgccggg gagcggcagg cgcggctgga tcttcacttt ttcagcgagg
     5221 aacgcgaaca ggccgcccgt atcctgattg aaaacggcaa gcggtcggca accctgaacg
     5281 aggtgccgaa acgctccgcc gccgggttga tcggttcgtt ctgcgcggtg atcttttccc
     5341 cggagcatct ttccctggta agggagggcc ccgctttccg gcgaaatttt atcgattccg
     5401 cgctgtgcca gattcagccg tcctacgcgg cgctgctgac ccggtacaac cggaccctca
     5461 ttcagagaaa ttccctgctg aaggatatcc cccgccacgc ccagcttttg gatacccttg
     5521 aaatctggga tgaaaaactg gtgcgatgcg gcatggaggt tgtgcggcag cgcatagaat
     5581 atatccgtca gatcggcgag ccggccgaag cggtttatgc cggaatctgt gaaaagaagg
     5641 aatcgctcgg tctaaattac ctgaaaagtg ccgaaaatct gaaacaggcg ctgtatgccg
     5701 caagagtttc ggatatcgcg ggcgggcaca cctccgtcgg gccccaccgc gacgatctgg
     5761 aaattcagat cgacggccgt tccgccaggg cgttcggttc acaggggcaa aagcgctccg
     5821 cggtactggc gctcaagctg gcggaagccg aaattctccg ccgaagaacc ggggaaaaac
     5881 cggtggttct tctggacgat gtgatgagcg agttggattc cggcaggcag gattatctgc
     5941 tgaaccacct ggaaaatttt caggtttttc tcacctgctg tgaaccggac gcggtaaaaa
     6001 ggctgaaaac gggccgtctt ttcgaaatgt cctcaggccg gctcgaacaa aaatgaaaca
     6061 gggaaaggtt tttttatgta tcttcatttg gggcaggaca cggtgattaa aattcaggac
     6121 atcatcggaa tcttcgatat ggaaacctca acaatttcga aggtgacgcg aaattatctg
     6181 gcggacgccc agaaagccgg acgtgttgtg aacgtttcca tggaaatgcc caagtcgttc
     6241 gtgctgtgct gcaaaaaaag cggtgaaatc accgtttaca tcacgcagat ttcgtcttct
     6301 acgctgctga agcgaaccgg atatatggaa gatatctcca atgtgtaaga aaggtggact
     6361 ccatgaaact gagcaaggtc cagtgcccct actgcggaaa aaagttaggc ctgacggccg
     6421 cctggatttt aagaacgcag ggtgaatatc attgtccgga ctgcggggcc tattcaaata
     6481 tcgaaatgtc cggcgggatt taccttttgg cggtaatcgc cgcgattttg agcgcgggcg
     6541 cgtttttggt ccagcttgtt ttagtccggt cgttcagctg gctgagtctg gccgtggttt
     6601 gcatcccgtt tatcctgttt tttctggctt cccccttttt tgtacggctg cagaagccgg
     6661 ccgtcagaag gcggcgccca tcgccccccc aggcgagcga tacccagaat tttaccccgc
     6721 ccaacgcatc ccccgggaac ccggccgact ccgaccggtt ggagcgcact atagtgatgc
     6781 gccggctgaa atagccgggt ggatttgatg ctgaaacttc cgctttgccc gtactgcggg
     6841 gcacgttttt tgtaccccac ggttaaggaa agtataaaat ataaaacaaa atcatgtccc
     6901 cactgtaata atacttataa aatcaacagc aagtataaat attttctttt tctggcggca
     6961 attctgctgc tcgttggcgt gaactggctg tttttaacga ttccctcgat gaatctgctg
     7021 tttttgacgg ggattacggc gctcggcgtg atcgcggctt attttctgat tccctacacc
     7081 atccggtacc aaaaaaccgg cccggccgcg gagcacccga aaaaaagccg gaaatagaag
     7141 aatgatgaat atacacagtg taaaaatact ggagggttaa acttggagtt tagcgaaatt
     7201 actcagacgc agcaaaaata tgatgccaat cagatccagg tgctcgaagg actggaggcg
     7261 gtgcgcaagc gccccggcat gtacatcggt tcgaccgggc cgcgcgggct gcatcatctt
     7321 gtttacgaaa ttgtggataa cgcaatcgac gaggcccttg ccggtttctg cgataaaatc
     7381 gacgtaaaaa tcctgcccgg cgacgtaatc agcgtgcgcg acaacggccg cggaatcccg
     7441 gtcggcatcc agccgaagct gggcatccct gcggttacgg tcgtttacac cgttctgcac
     7501 gcgggcggca agttcggcgg cggcgggtac aaggtttccg gcggactgca cggcgtgggt
     7561 gcttccgttg tgaacgcgct ttccgagtgg ctcgaagtgg aagtctatgt ggacggaaaa
     7621 atttactgcc agaggtttga acgcggcaag gccgtgaacg aactcaaaca gtgcggcgac
     7681 accgaacaga ccggaacgct ggttcgcttt aagccggatt cggagatttt tcaggaaacc
     7741 acggcatttg actacgaaac ccttcagacc cgtctgcgcg aacaggcgtt tttaaacgcg
     7801 ggcattcata tcgaactggt cgacgaacgc gacccgcaga accggattca gaacaattac
     7861 tgctaccacg gcggcatcag cagctttgta gaatttatca acaagaaaaa agcggtggag
     7921 accgtccacc cggacatcat ccacttttct gcggttgcgc cggacgacaa cgcgactgcc
     7981 gaagtagcta tgcagtacaa cgattcctat aacgagctga tgctttcgtt tgcgaataat
     8041 atccatacca ccgacggcgg cacgcatgag gacggcttta agcgggccct cacccgcgtg
     8101 atgaacgatt acgcgcgaaa atataatatt ctcaaggaaa atgataaaaa cctgtccggc
     8161 gaggatgtgc gcgaaggtct gacggtcatt atcagcgtca agctgaagga ggcgcagttc
     8221 gaaggccaaa ccaaggcccg cctcggcaat acggaaatcg ccacgctggt gggcaacatg
     8281 atctccgaaa agctgtccac ttacctggaa gaaaaccctg cgacggcccg cgcgattttc
     8341 gataaggctc tcgcagcctc ccgcgcgagg gacgccgccc gcaaggcgcg cgatcttgtg
     8401 cgccgcaaat ctgcgctgga aaacgccgcg ctgccgggca agctggccga ctgccagagc
     8461 cgcaacccgg acgaaaccga aatttacatc gtcgaaggag attccgccgg cggctccgcc
     8521 aaaagcggac gtaaccgcaa atatcaggca atcctgccgc tctggggcaa aatgctcaac
     8581 gtggaaaagg cccggctcga caaggtttac ggcaatgaaa agctgatgcc ggtcgttacg
     8641 gcgcttggct gcggcatcgg cgaggaattt gacctcagca agctgcgcta cggcaaaatc
     8701 atcatcatgg ccgatgccga tgtcgacggc tcccatatcc gcacgctgct tctgactttc
     8761 ttcttccgct ttatgaaacc ccttgtggag gaaggccacg tttacctggc gcagcccccc
     8821 cttttccgcc tgacaaaagc gaaaaaccac tactatgcct acacggaccc ggagcgcgac
     8881 cggctgatga cggagctcgg cagcaactac gaaatccagc gttacaaagg tctgggcgaa
     8941 atggacgccg aacagctttg ggagacaacc atgaatcccg aaacccgcac catgctccgc
     9001 gtggaagtgg aggacgctgc cgccgcggac gaagcgttca ccatcctgat gggcgacaag
     9061 gtggaacccc gcaaggaatt cattgagaaa aacgcaaaat atgtgacgaa tctggatatt
     9121 tagattatac tctttgtata tttattatat attatacatt aaggagcatt attatgaaaa
     9181 agtttatcag tgaacaggac tcggccgcaa aacctccgga accccagggg gaacggaccc
     9241 ccgccgaagc ggacgaaagt ctgccggaat acgaatccgg agaagaactg ctggatgaaa
     9301 ccatggagga actgcaggaa aacgaggata ccgaagagcc aatcgaatgg gacggctccg
     9361 aagcggagct ggttgccgac gaggacgccg cggaatacga ggaacagaac accgggattc
     9421 agctcaaata tgtgctgaaa tcacaggaga ttttccgcgt ccttttcaaa acgcagatga
     9481 cgccgaaacg aacggcgctg accgtaaccg ccgcgctgct gggtgtagcg ctgggcgttt
     9541 acttcctgat acggactgtg aacggggatc acatcgcggg ttcgccgggc gcaatctgcc
     9601 tgattcttgc cgggatcatc cttgcctccc ccgttctgca catccgttcc caggccaaaa
     9661 attcggaaaa cggaaaagaa atccgcatga agatttatcc cgaccatatt caaatgggcc
     9721 gtggggaagg cggatgggaa attccgctgg acggcacggt ggtgcgcaat gtgctgaaag
     9781 ggctgatttt cttttacata gacggaaaca ggctggtgat cctgccgctt cgctgcgtgg
     9841 agccttccgt tctgcccgaa gtgcaggcca tgatttttgc gggaaccagc ccgtccagat
     9901 aacggaggaa ctccggtttt caaaggttcc tcggaaaagc aaaggaaggt ttatgccgac
     9961 attttacgaa ggaaacccga tcgagacggt cgaacagatt atcagcgaac aggattacgc
    10021 cgatgcttac tacgcggcgc acagttcttc cgcactgctg caccgcaggg acgtgcaggc
    10081 gggaatctgc ctgtcgctgg cggtgctgtg cgcttcggtc atcccgcttt accgggcaag
    10141 gttttccaca ttctgggggc ctgtctgtgg aattcttgcg tttctcgcgc ttgcggcggt
    10201 gttttttttc cttaagccgc aggacacgaa gaactgggcc cgcaggcttt accgttccaa
    10261 cgcgctgatg gccctgccgc agaaggtgac tgtttaccgc gacagcatcg ttttggaaaa
    10321 cgggcgggaa cggttttcgg aatattggac ggacttttcg ggctgtgtgg aaacgaaaga
    10381 cgcctttatc ctgaccggag ggcgtgaacg gcttttgctg ctcattaaaa aaaagggctt
    10441 aagccccgaa aggcaaaata tgctttccgc tcattttgcc gacgcgtttg cctaccggtt
    10501 ccgaaaattc ggccgccagg gagggacctg attttgaaaa atcccatctc cgtcagtttt
    10561 ctggttactg aaccggattt tgttgatttt aaaacggccg ccgccaaggc ctgcgtaaaa
    10621 aagagtgaaa tttacctgct ccgggtcacg ggctgcgtgc tgatttttac ggccttggtt
    10681 ttatgcgcgt tttacagtgg cccgtttggc cgaaacgcgc tttacatttt gatcgcggca
    10741 gccggcgccg tgctgggcgc ctttcacgat ttgacgattc actgtcttgt ccgccggcag
    10801 gcggcctcct tttacgagga gaacaaagaa aaattcggcg cgcagaatac ctaaaacagc
    10861 gaccggtgcg tccggtttcc ctccgacagt tattcggcgg agctgccg
//
LOCUS       DATALY010000065         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_792347,
            whole genome shotgun sequence.
ACCESSION   DATALY010000065 DATALY010000000
VERSION     DATALY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792347"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VHR42_02005"
     CDS             complement(<1..607)
                     /locus_tag="VHR42_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3025991.1"
                     /translation="METIRKHFFSDIGVMLGRSMRHISRSMDTIITVTIMPIAFMLLF
                     VYVFGGAIQAGTDNYVNYLLPGILLIAIASGIAYTAYRLFMDMQRGIFERFQSMPIAR
                     SAALWGHVLTSLVSNAISVVVIILVALIMGFRSSAGVLSWLTVAGILALFTLALTWIA
                     AIAGLSAKSVDGAGAFSYPISPYYFVLSLTFFMALLTSCSTD"
     gene            complement(608..1366)
                     /locus_tag="VHR42_02010"
     CDS             complement(608..1366)
                     /locus_tag="VHR42_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3025992.1"
                     /translation="MEKAIEVKGLQKSYKKLHVLKGVDFKVEKGSIFALLGSNGAGKT
                     TIVKILTTLLKQDSGTAAVNGFDVTEKPDYVRQSISLTGQFAAVDEILTGRENLIMIA
                     KLRHLTNPRQVADDLLNRFGLTDAADRRVSTYSGGMRRRLDIAMSLIGKPQLIFLDEP
                     TNGLDPEARIETWKIVKELADGGTTVFLTTQYLDEAEQLADRIAILHEGKIIANGTLA
                     DLKKLFPPAKVEYVEKQPTLEEIFLAIIGKKEGK"
     gene            complement(1382..1732)
                     /locus_tag="VHR42_02015"
     CDS             complement(1382..1732)
                     /locus_tag="VHR42_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HEX3025993.1"
                     /translation="MRIQDIIEGKKEWRAHVARVKALPQDYQIVYKEIQKYLFKVGPV
                     ELTDGTGLLSGIIDLFEEGAALGKGVLEVTGSDVAAFCDDLIKDSKTYADIYQESVEQ
                     EVNKAMKKVRDKTK"
     gene            complement(1742..2089)
                     /locus_tag="VHR42_02020"
     CDS             complement(1742..2089)
                     /locus_tag="VHR42_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HEX3025994.1"
                     /translation="MNFYEKITGSDMTKEMKTFESRAKKLPADYQAAWEKIKANLWQH
                     SDFTGRNLMPILDGVLGLLEETAADGQSAQEVLGDDIKGFCSALVGEEGAKSFRDKWR
                     EQLNNNIAKKLGR"
     gene            complement(2089..>2221)
                     /locus_tag="VHR42_02025"
     CDS             complement(2089..>2221)
                     /locus_tag="VHR42_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX3025995.1"
                     /translation="KPSDMGPPRKFFVLNDAGRKELQKFWEKWGFVSSKINELKEKI"
BASE COUNT          542 a          582 c          485 g          612 t
ORIGIN      
        1 aatctgttga acaagaagtt aacaaggcca tgaaaaaggt tagggataaa acaaagtaat
       61 agggggatat cggataggaa aaggctcctg caccgtccac cgacttcgcg gacagtccgg
      121 caatcgccgc gatccatgtc aaagccagcg taaacagcgc gagtataccg gctacagtaa
      181 gccatgacaa tactccagcc gacgagcgga agcccataat gagagcaaca agaatgatta
      241 cgacaaccga aatcgcattg gataccagcg aggtcagcac atgcccccac agcgcagccg
      301 aacgcgcaat cggcatggat tggaaccgtt cgaagatgcc tctttgcata tccataaaca
      361 gacggtaagc cgtataggcg atgccgcttg caatcgcaat aagtagaatg ccgggcagca
      421 ggtaattcac atagttgtcc gtgccggctt gaatcgcacc gccgaacaca tagacgaaca
      481 gcagcataaa cgcaataggc atgattgtga ctgtgatgat ggtgtccatg ctgcgtgaaa
      541 tatggcgcat ggaacgtcca agcataactc ctatatcact gaaaaagtgt ttcctgatcg
      601 tttccattta cttaccctcc tttttgccga tgattgcgag gaatatctct tccaatgtcg
      661 gctgtttctc aacatactcc acctttgcgg gcgggaacag ctttttcaga tctgcgagcg
      721 tgccgttagc aataattttc ccttcatgca gaatggcaat tctgtcggcg agctgttcag
      781 cttcatccaa atactgtgta gtcagaaata ccgtcgtacc gccgtcggca agctccttta
      841 caattttcca agtctcgatg cgcgcctctg ggtcaagccc gttggtcggc tcgtcgagga
      901 aaatgagctg cggttttccg ataaggctca tggcgatgtc taatctgcgg cgcataccac
      961 ccgaataggt ggaaactctg cggtcggctg catcggtcaa gccgaagcgg ttcagtaaat
     1021 catccgcaac ctgtcgcgga tttgtaaggt gacgcagctt ggcaatcatg atcaaatttt
     1081 ctcgcccggt caagatctcg tctacagcgg cgaattgccc tgtcaggcta atcgactgcc
     1141 gcacatagtc gggtttttcc gtaacatcga atccgtttac ggcagcagtt ccactatcct
     1201 gcttgagcaa cgtggtgagg attttgacaa tcgttgtttt gcccgcgcca ttggagccga
     1261 gcagggcgaa aatactgcct ttttccacct taaaatctac gccctttaaa acatgaagtt
     1321 tcttgtagga cttttgcagt cctttcactt caattgcttt ttccatcccg atatccccct
     1381 attactttgt tttatcccta acctttttca tggccttgtt aacttcttgt tcaacagatt
     1441 cttgatagat gtcagcgtaa gtttttgaat ctttgattag atcgtcgcag aaagccgcta
     1501 cgtcactgcc cgtcacttcg agcacgcctt tccccaaggc cgcgccctct tcaaaaagat
     1561 cgataatccc cgagagcaaa cccgtcccgt cggttagctc aacaggtccg accttaaata
     1621 gatatttttg aatctcttta tacacaatct gataatcttg cggaagcgct ttgacacgcg
     1681 ctacgtgcgc tcgccactct tttttgcctt cgatgatatc ctgtattctc atgttatcct
     1741 cctatctacc taatttttta gcgatattat tgttgagttg ctcgcgccat ttgtcgcgaa
     1801 aagactttgc cccttcttcg ccgaccagcg ctgaacagaa gcctttgata tcgtcaccca
     1861 aaacctcttg ggcactctga ccgtccgccg ccgtttcttc gagcaggcca agcacaccgt
     1921 caagaattgg cattaagtta cggccagtga aatctgagtg ctgccaaaga ttggccttaa
     1981 ttttttccca tgctgcttga taatcagccg gcagcttttt ggctcgcgat tcaaaagttt
     2041 tcatttcttt agtcatgtcg ctgcctgtaa ttttctcata aaaattcatt atattttctc
     2101 ctttaactca ttaatttttg atgatacgaa cccccatttt tcccagaatt tctgtagttc
     2161 cttgcgcccc gcgtcgttga gcacgaaaaa tttgcgcggc ggtcccatat cggatggttt
     2221 t
//
LOCUS       DATALY010000066         9169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_705446,
            whole genome shotgun sequence.
ACCESSION   DATALY010000066 DATALY010000000
VERSION     DATALY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705446"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VHR42_02030"
     CDS             complement(<1..385)
                     /locus_tag="VHR42_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX3025996.1"
                     /translation="MIRSHVLVCGGTGCTSSGSEQIIKTLESEIKKQGLDEEVKVVRT
                     GCFGLCALGPIMIIYPEGAFYSMVKIEDIPEIVSEHLLKGRIVKRLLYKETVAEDNIK
                     SLNETPFYKKQLRRLFFGCRQNQGRG"
     gene            complement(399..770)
                     /locus_tag="VHR42_02035"
     CDS             complement(399..770)
                     /locus_tag="VHR42_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX3025997.1"
                     /translation="MKSLAELAEIRDKMKGKVNIRENSGDTIRVVVGMATCGIAAGAR
                     PVLNTMVEEVSKRKLENVMVTQTGCIGICQYEPVVEVYEQGKEKVTYVKMTAEKALRV
                     VSDHLVNGNVVMEYTIGAAAK"
     gene            complement(799..1356)
                     /locus_tag="VHR42_02040"
     CDS             complement(799..1356)
                     /locus_tag="VHR42_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3025998.1"
                     /translation="MMQEISLNILDIAQNSVSAGASLIEITVDEQPGPDSLIVTIRDN
                     GKGMTPEQVEAVTDPFYTTRTTRKVGLGVPFFKMAAEMSGGSFSIRSKVGEGTVVCGK
                     FGLSNIDRMPIGDVNATILTLITCNPGLDFVYTRRKGGGEFILDTREVRNVLGEVPLN
                     NGEVTLWIRDFLREGEAEVAPDNPQ"
     gene            complement(1365..1859)
                     /locus_tag="VHR42_02045"
     CDS             complement(1365..1859)
                     /locus_tag="VHR42_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX3025999.1"
                     /translation="MQKRKTSVPFKGTKEQEEQLLAVIEKHKTDKGSLMPILQEAQEI
                     YGYLPIEVQQIVADSMGKSLEEVYGVISFYSQFSLSPKGQYQISVCLGTACYVKGSNK
                     ILDKISDKLGIKPGECSADGKFSLDATRCIGACGLAPVMTVNDEVFGRLVEDDVEKIL
                     AKYN"
     gene            complement(1967..2698)
                     /locus_tag="VHR42_02050"
     CDS             complement(1967..2698)
                     /locus_tag="VHR42_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3026000.1"
                     /translation="MTGSGQFCYDFHIHSCLSPCGDADMTPNNMVNMAKLNGLDAIAI
                     TDHNTCKNCGAAVKAGERAGLLVIPGMELCTREEIHVICLFPDVERAESFAGFIYDRL
                     PGIPNRSDIFGDQLVLDDMDRVTGSEEKMLLNAVDIGVDSVKALVSEYDGAAFPAHID
                     KESYSILGVLGSIPPETGFFCAELSTLCERERFLADHPELSEFRFVVNSDAHYLWQIS
                     EREHSMSGEVSAGAILLSMNRQKIL"
     gene            complement(2695..3030)
                     /locus_tag="VHR42_02055"
     CDS             complement(2695..3030)
                     /locus_tag="VHR42_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026001.1"
                     /translation="MTVKQIAEYLECRTICGGNALDKQVKDGYSGDLLSWVMGRAPAG
                     SAWITVMGNVNAVAVAVLADAACIILAEGAVLDGEAAAKALSNNVAVLCSDRTSFDLA
                     SNLSRIFRL"
     gene            complement(3020..4390)
                     /locus_tag="VHR42_02060"
     CDS             complement(3020..4390)
                     /locus_tag="VHR42_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX3026002.1"
                     /translation="MGEFYHSVTLDSSRCKGCINCIKRCPTEAIRVRNGKAYIISERC
                     IDCGECIRVCPHHAKKAVFDPLSSIDRYKWKIALPAPAIYGQFNNLDNIDKVLTALKK
                     IGFDDIYEVSSAAEIISECTRRMMLAGLVQKPVISSACPAVTRLIRVRFPNLCRNVLP
                     LLAPIELAARTAKEEAAEKTGLKPEEIGVFFISPCPAKVTYAKSPLGIKKSAVDHVLS
                     IGDIYPKIVGIIKHMKNPEALSKSGIIGVGWAGSGGESSALLSEKYLAADGIENVIKV
                     LEELEDEKLGEIDFIELNACSGGCVGGVLTVENPYVAKARIHRLRKYLPVAMNHLEGD
                     VPASILWNIPLEYVPVMKLDQDVSAAMKKMIEIDSLAENLPGLDCGSCGAPSCRAFSE
                     DVVRGMATEGDCVFKLRERIHKLVDELSELEGYVPPPFRKSESDGGQKNGDVSSNQGK
                     DVTHDG"
     gene            complement(4428..4856)
                     /locus_tag="VHR42_02065"
     CDS             complement(4428..4856)
                     /locus_tag="VHR42_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026003.1"
                     /translation="MNESMHLHFDIPSDDFTHAGEASGNVKKVLKQLGFAPDVIRRVT
                     ISMYEGEINMVIHAGGGMAEVYITPEKIEIKLIDQGPGIPDIGLAMQAGYSTAPDNIR
                     SLGFGAGMGLPNMKKYTDQLEIESEVGKGTSVHMTVDVKG"
     gene            complement(4860..5204)
                     /locus_tag="VHR42_02070"
     CDS             complement(4860..5204)
                     /locus_tag="VHR42_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HEX3026004.1"
                     /translation="MTVREIIQILHANVITGENNLESEVKSACGSDMMSDVLAFVKNQ
                     SVLLTGLVNPQVVRTAEMMDMKCIVFVRGKSPDGSIVKLADEKGIVLLNTKERMFTAC
                     GLLYSSGLKGAE"
     gene            5744..6826
                     /gene="serC"
                     /locus_tag="VHR42_02075"
     CDS             5744..6826
                     /gene="serC"
                     /locus_tag="VHR42_02075"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX3026005.1"
                     /translation="MKRVYNFSAGPSMLPEPVLKRAAEEMLDYGKSGQSVMEMSHRSK
                     IYDSIITGCEELLRKVMNIPSNYKVLFLQGGASSQFSMIPLNLMNGSGKADFVITGQW
                     AKKAYQEASRYGKCNVVASSADKTFTYIPELDPNKFDKEADYFHICYNNTIYGTKFVS
                     LPDTGKVPLVADVSSCILSEPIDVSKFGLLFAGAQKNMGPAGLTVVIVREDLIGHARD
                     ITPTMFNYQIHADNKSLYNTPPCYSIYICKLVLEWLQELGGIEKMAEINREKAGLLYD
                     YLDSSKLFRGTVEKEFRSLMNIPFITGNEETDKKFVKEAEAAGFINIKGHRTVGGMRA
                     SVYNAMPVQGVRDLVAFMKDFEAKNA"
     gene            6893..8059
                     /locus_tag="VHR42_02080"
     CDS             6893..8059
                     /locus_tag="VHR42_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX3026006.1"
                     /translation="MFSIRTMNKISPEGLSRFEPGAFTCSDDAQKPDALMVRSASLHE
                     MEMPSSVKAIARAGAGVNNIPVDQCSKEGIVVFNTPGANANAVKELVLGAMIFSARNV
                     INAVEWAKTLKGSGAEVGKLVEKGKSKFAGPELSGKKLGVIGLGAIGVMVANDAHSLG
                     MEVYGFDPYISVDAAWGISRAINRANSMKEIFEKCDFITIHVPLNPETRGTFNAEAFA
                     AMKKGVRLFNFARGELAVSEDVAEALASGQVASYTVDFPCDELLGVPGVISIPHLGAS
                     TPESENNCASMAADELIQFLKTGTIRNSVNFPNADMAFSSATRITVIHKNIPNMLSQI
                     STAFAQENINIENMLNKSKKEYAYTMLDIDGEAIDKAVEKLRAIDGIIRVRVIR"
     gene            complement(8153..8476)
                     /locus_tag="VHR42_02085"
     CDS             complement(8153..8476)
                     /locus_tag="VHR42_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026007.1"
                     /translation="MEEYKKDVRNVLKHLGVLNGEPELNGKTPVEVTGVVYLRATNAG
                     CWYPCVTANEKVAVGQKLGEVRDFFGKVLDTYYSEINGVVLYMTVSLAVRKRAHLVAY
                     GKLPE"
     gene            8548..>9169
                     /locus_tag="VHR42_02090"
     CDS             8548..>9169
                     /locus_tag="VHR42_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX3026008.1"
                     /translation="MLVHPVNTQGFNARGSDIVPEDGKNINRVFPGDRDGTITDQIAY
                     VLTHEFQDEADFYIDIHGGDLNELMTPLAFFPGAAEREVTEVSLGAAKSLRVEYLVRS
                     GAKTGAYNSAAIRGVPSILIERGGRGLWGREEVEEYKKDVRNVLKHLGVLNGEPELNG
                     KTPVEVTGVVYLRATNAGCWYPCVTANEKVAVGQKLGEVRDFFGKVL"
BASE COUNT         2242 a         2498 c         2133 g         2296 t
ORIGIN      
        1 aaccccggcc ttgattttgt ctacacccga agaaaaggcg gcgaagctgt tttttataga
       61 acggtgtctc attcagcgac ttgatattat cttccgcgac ggtttcctta taaagcaggc
      121 gtttgacaat tcttcccttg agaagatgct cgctgacaat ctcagggata tcctcaatct
      181 taaccatact gtaaaaggcg ccttccgggt agataatcat aatcggaccc aaagcgcaaa
      241 ggccaaagca accggttctg acaaccttta cttcttcgtc aaggccctgt ttcttaattt
      301 cactttcaag cgtttttatt atctgctcgc tgcctgacga ggtacagcca gtgccgccgc
      361 agacaaggac atgcgaccga atcatttatg aaacctcctt atttagcggc tgcgccgatt
      421 gtatattcca tcactacatt gccgttgacc agatgatcgc tgacgactct taaagccttc
      481 tccgcagtca tcttgacata agtgaccttt tcttttccct gctcgtaaac ttccacaacc
      541 ggttcatact ggcagatgcc gatgcagccg gtctgggtta ccattacgtt ttcaagcttc
      601 cgcttggaaa cttcctcaac catggtgtta agaacgggac gtgctcccgc ggcgatgccg
      661 caggtagcca ttccaacgac aacacggatc gtgtcgccgc tgttttcgcg tatattgacc
      721 ttgcctttca ttttatctct gatttcggca agctctgcaa ggctcttcat aaaaacacac
      781 ctttctttac gattcttatt actgcgggtt atccggggca acttccgctt cgccttcccg
      841 gagaaaatcc ctgatccaaa gcgttacttc accattattc aggggaacct cccccaaaac
      901 attccgtacc tcacgggtgt caagaataaa ctccccgccg ccttttcttc gggtgtagac
      961 aaaatcaagg ccggggttgc aggtaatcag tgtgaggatt gtcgcgttca catcaccgat
     1021 cggcatccgg tcgatattgg aaaggccaaa ttttccgcag accacagttc cctctccgac
     1081 ctttgaacgg atggaaaaac tgcctcccga catttcggca gccatcttaa aaaaaggcac
     1141 tccaagcccg acttttcgcg ttgttctggt tgtgtaaaac ggatcggtca ccgcctcaac
     1201 ctgttcgggt gtcatacctt ttccattgtc gcggatcgtg acgatcaggc tgtcagggcc
     1261 cggctgctca tccacggtga tctcgatcag ggatgcgccc gccgaaacgg aattctgggc
     1321 aatgtccaga atattcagtg atatttcctg cataatttta aaatttaatt atatttcgcg
     1381 aggatctttt cgacatcatc ctcaacgagc ctgccgaaaa cttcatcatt gaccgtcatc
     1441 acaggtgcaa gtccgcaggc gccgatgcag cgggtggcat ccagtgaaaa ctttccgtcg
     1501 gcagagcatt ctcccggctt aatcccgagt ttatccgaga ttttgtcaag tattttgttt
     1561 gagcccttaa cgtagcatgc cgttccaaga catacagaaa tctggtattg acctttcggg
     1621 ctcaaagaga actgtgagta aaagctgata acaccataga cttcttccag cgatttcccc
     1681 atactgtcag ccacaatctg ctgaacctcg atcggcagat aaccgtagat ctcctgagct
     1741 tcctgcagaa tcggcatgag tgagccctta tccgtcttat gcttttcaat gacggcaagc
     1801 agctgttcct cctgctcctt tgtccccttg aaaggaacac tggttttgcg cttttgcatg
     1861 acttttcctc ctacggatat agtttgttaa attattgaca agtgttcacc aacaaaaacg
     1921 attatatcac aaaattcggc cacaggccat atttattcaa gaaaaattaa aggatttttt
     1981 gtcggttcat ggataataga atcgcaccgg ctgatacttc gccggacatc gaatgctccc
     2041 gctcgctgat ctgccagaga taatgcgcgt cggaattgac aacaaaacga aattcggaaa
     2101 gctccggatg atcggccaga aaccgttccc gctcgcaaag cgttgaaagt tccgcgcaga
     2161 aaaatcccgt ttccggagga atggaaccca gcacccccag aatactataa gactccttat
     2221 caatatgcgc gggaaaagct gcgccgtcat attccgaaac cagtgctttc acactgtcaa
     2281 cgccgatatc cacagcgtta agcagcattt tttcttcact gcccgtcacc cggtccatgt
     2341 cgtcgagaac cagctgatcg ccgaatatgt cggatcggtt tggtatcccg ggcagcctgt
     2401 cgtatataaa accggcgaaa gactcggccc gctctacatc cggaaacagg cagatcacat
     2461 ggatttcctc tctggtgcac agctccattc cggggatcac cagcagcccg gcccgctcgc
     2521 ccgctttaac cgccgccccg caatttttgc aggtgttgtg gtcggtaatc gcgatcgcgt
     2581 ccagcccgtt caatttcgcc atatttacca tgttgtttgg cgtcatatcg gcgtcgccgc
     2641 agggcgacag gcaggaatgg atatggaaat cataacaaaa ctgccctgat cctgtcatag
     2701 ccggaatatc cttgaaagat tcgatgccaa atcgaaagac gtacggtcgc tgcaaagcac
     2761 cgccacgtta ttggagagcg cctttgcggc ggcttcaccg tcgagtacgg ctccctccgc
     2821 cagaatgata caggcggcgt ctgcaagcac ggccaccgcc acggcattga cgttgcccat
     2881 tacggtgatc caggcgcttc ctgctggcgc cctgcccatc acccagctta acaaatcccc
     2941 gctgtagcca tccttcacct gcttgtcaag ggcatttcct ccgcagatcg tcctgcattc
     3001 caaatattcc gcaatttgct taaccgtcat gggtgacatc ctttccctga tttgaagaga
     3061 catcgccgtt tttctgaccg ccgtcgctct ccgacttacg gaacggcgga ggcacatagc
     3121 cctccagttc ggacaattcg tcgacaagtt tatgaatccg ctctcggagc ttaaaaacgc
     3181 agtcgccctc ggtcgccatg ccgcgaacga catcttccga aaacgcacgg caggacggag
     3241 ccccgcagga accgcagtca agtccgggaa gattctcggc taagctgtcg atctcaatca
     3301 tttttttcat cgccgccgaa acatcctgat caagcttcat caccggcaca tattcgaggg
     3361 ggatgttcca cagaatcgag gcgggtacat cgccttccag atgattcatc gcaacgggca
     3421 gatatttgcg cagccgatgg atccgggctt tcgccacata gggattttcc acggtaagaa
     3481 ctccgccgac acagccgccg ctgcaggcgt taagctcaat aaagtcaatc tcgcccaact
     3541 tctcgtcttc aagttcttcc aaaactttaa tgacattttc gatgccgtcg gccgcgaggt
     3601 atttttcgct gaggagggcg gaagattcac cgcccgagcc ggcccagcct accccgataa
     3661 tcccggattt cgacagagct tccggatttt tcatatgttt gatgattccg acgattttcg
     3721 ggtaaatatc accgatggat aaaacgtgat ccactgcgga ctttttaatt cccaggggag
     3781 atttcgcata agtaacctta gccgggcagg gcgatataaa aaacaccccg atttcctccg
     3841 gtttgagacc ggttttctcc gcggcttctt ccttggccgt tcgcgcggcg agctcaatag
     3901 gagccagcag cggcagaaca ttccggcaga gatttggaaa cctgacccgg atcagccttg
     3961 tcaccgccgg gcaggccgag ctgatcaccg gcttctgaac aagccccgcc agcatcatcc
     4021 gccttgtgca ttcggatata atctcagcgg cgctgctgac ctcgtagata tcgtcaaatc
     4081 caattttttt cagcgcggtc agcaccttgt cgatattgtc cagattatta aactgcccat
     4141 aaatcgcagg agcgggcagg gcaatcttcc atttataccg gtcaatgctg gaaagcgggt
     4201 cgaacaccgc ctttttcgcg tggtgcgggc acacccggat gcattcaccg cagtcgatgc
     4261 atctttccga tataatataa gccttgccgt tgcgcaccct tatcgcttcg gtcgggcatc
     4321 ttttaatgca gttgatacag cctttgcatc gggagctgtc gagcgtaacc gaatgataga
     4381 attctcccat ccgtcaaaca cctccatcca tttgcggcac cagtgcttta acccttcaca
     4441 tctactgtca tatgtacgct ggttcccttg ccgacctccg attctatttc cagctggtcg
     4501 gtgtattttt tcatgttggg aagacccatt ccggccccaa agccaagaga gcggatattg
     4561 tcaggcgctg tggaataacc tgcctgcatg gcaagaccga tgtccggaat acccgggccc
     4621 tggtcaatga gtttaatctc tattttctcc ggcgtaatat aaacctccgc catccccccg
     4681 cctgcgtgga taaccatatt gatttcacct tcgtacatcg agatggtgac ccggcggatt
     4741 acatccggag cgaaacccag ttgtttaagc acctttttca catttccgga cgcttcccca
     4801 gcatgggtaa aatcatcgct cggaatgtca aaatgcagat gcattgattc attcattttt
     4861 tactccgccc cctttaaacc actcgagtaa agaagaccgc aagccgtgaa cattctttct
     4921 ttcgtgttga gtaaaacaat tcctttttca tcggcaagtt taacaatcga cccgtccggc
     4981 gatttccccc ggacaaaaac aatgcacttc atatccatca tttccgccgt acggacaacc
     5041 tgcggattga caagaccggt caacagaacc gactgatttt taacaaacgc caaaacatcg
     5101 ctcatcatat cgctcccgca ggccgatttc acctccgact ccagattatt ttcgccggtt
     5161 atgacattgg cgtgcaatat ttggatgatt tcccgcaccg tcatgccgta gccctcccaa
     5221 ttttcatatt atgatgattt cccctgatcg cggcggccgg ctgatccgat gaccgtcttc
     5281 agccggtaac aggccgaccg tgtgcaatta cacggcacct atcttctcgt ttgcgccgtc
     5341 cgaattttct ctgctttttc tgaaatgttt tctatatttt aaccgtccgc ttcatgaagt
     5401 atggcggcat cgccggccga accggtacgg tttctgtttg ccgcttaata ttatatcatt
     5461 tatcgaagca cttcacaaca ttttgcggcc ctgccggaag cgtaacctca tctttccaaa
     5521 aaggccaata agaaatcaga aaaagtttgt tataatttta tcataataac acaatttttt
     5581 gctttatcac gtaaaattat tgacttgaac aacagcttag tactagaata gttatattgt
     5641 catagccttt gccaagcaat ctgtgcatat ggcatggacc ggccgcgaat cacggttgat
     5701 ccgaattaca gccttaaggg caatcaaaca ggaggtatcg caattgaaaa gagtttacaa
     5761 cttttccgca ggcccgtcaa tgctgcccga gccggtttta aaacgcgcgg ctgaggaaat
     5821 gctggactat ggcaagtccg gccagtccgt tatggagatg tctcatcgat caaaaatcta
     5881 cgacagcata atcactggct gcgaagaact tctccgtaag gtcatgaaca tcccctcaaa
     5941 ctacaaggtt ctcttccttc agggaggcgc ttcaagccag ttttcaatga taccgctcaa
     6001 cctgatgaac ggaagcggaa aagccgattt tgtcatcacc ggccaatggg cgaaaaaagc
     6061 ctatcaggaa gcttcacgtt atgggaaatg caacgttgtt gcatcttccg ccgacaaaac
     6121 ttttacatat atcccggaac ttgacccgaa caaattcgat aaagaggccg attatttcca
     6181 tatttgctat aacaacacca tttatggaac gaaattcgtc agtctccccg acaccggcaa
     6241 ggttccgctg gtggctgatg tttcgtcctg cattctgtcc gagccgatcg atgtctctaa
     6301 attcggtctg ctttttgccg gagcgcaaaa aaacatgggc cccgccggac tgaccgtagt
     6361 cattgtccgg gaagacctga tcggccacgc ccgtgatatt accccgacca tgttcaacta
     6421 tcaaatccac gcggataaca aatccctcta taacacgcct ccctgctact cgatttatat
     6481 ctgcaaactg gttctcgagt ggctgcagga actcggcggc attgaaaaaa tggccgagat
     6541 caaccgggaa aaggcgggcc tgctttacga ttatctggat tcttcaaaac tcttccgtgg
     6601 aacggtggaa aaagaattcc gctcgctgat gaacattccg tttattacgg gcaacgaaga
     6661 gacagataag aagtttgtaa aggaagccga ggccgccggt ttcatcaaca tcaagggcca
     6721 ccgtacggtg ggcggcatgc gggcaagcgt ttacaacgcc atgccggtac agggagtccg
     6781 cgatctggtt gcgttcatga aagattttga agcaaagaac gcttaaataa tttagaatta
     6841 acaaaagggg cgctgaaaac gccccttaaa tttcatatgg gaggtttgac ctatgttctc
     6901 gatcagaacc atgaataaaa tatcgcccga aggcctttcc cgttttgagc cgggcgcttt
     6961 cacatgctcg gacgatgcgc agaaaccgga tgcgctgatg gtccgctcgg catcccttca
     7021 cgagatggag atgccttcct ctgtcaaggc gatcgccaga gccggagccg gtgttaacaa
     7081 catcccggta gaccagtgca gcaaagaagg gattgttgtt tttaacactc ccggagccaa
     7141 tgccaacgcc gtaaaagagc tggttctcgg cgctatgatt ttctcggcaa gaaacgttat
     7201 caacgccgtg gaatgggcca aaaccctcaa gggcagcgga gccgaggtcg gcaagctcgt
     7261 tgaaaaagga aaaagcaaat ttgcgggccc tgaactttcc ggtaagaagc tcggcgttat
     7321 cggccttggc gccattggcg ttatggttgc aaacgacgcc cacagcctcg gcatggaagt
     7381 ctacggtttc gacccctata tttcggtaga cgcggcttgg ggcatctcaa gggccatcaa
     7441 tcgtgccaac agcatgaagg aaatctttga aaaatgtgat ttcatcacca ttcacgttcc
     7501 gctgaatccg gaaacccgcg gaactttcaa cgccgaagcg ttcgcagcca tgaaaaaggg
     7561 cgtgcgtctg tttaatttcg ccagaggcga gctcgcggtc agcgaggatg tcgctgaagc
     7621 gcttgccagc gggcaggtgg cctcctatac ggtggatttc ccctgtgatg agcttctggg
     7681 cgttcccggc gttatttcca tcccccatct cggcgcttcc acacccgagt cggaaaataa
     7741 ttgcgcctct atggcggcag acgagttgat acagttcctg aaaacaggaa cgatccggaa
     7801 ttccgtcaac ttccccaatg ccgacatggc attttcttcc gccaccagaa tcaccgtcat
     7861 tcataaaaat atccccaaca tgctttcgca aatttccacg gctttcgccc aggaaaacat
     7921 caacattgaa aacatgctga acaaatccaa aaaagaatat gcctatacga tgctggatat
     7981 cgacggcgaa gcgattgaca aagcggtcga aaaactccgt gccatcgacg ggatcatccg
     8041 cgtacgggta atccgctgat cttcttatat actgcagaaa cggacgaaag cgttggcttt
     8101 cgtccgtttt ttataccctg accggtttgg ttgtcaggct attcgtctgt tattattccg
     8161 gcaattttcc gtaagcgacc agatgggctc tctttcttac cgccagggaa acggtcatat
     8221 agagaacaac tccgttgatc tcggaataat aagtatcaag aactttgccg aaaaagtcac
     8281 gaacctcgcc cagcttctgg ccgacggcga ccttttcatt cgcagtcaca cagggatacc
     8341 agcagccggc atttgtcgcc ctgagataga ccacgccggt aacttcaacc ggagttttcc
     8401 cgttcaattc cggctctcca ttcaaaacgc ccaaatgctt taacacgttt cggacgtctt
     8461 tcttgtattc ctccacttcc tccttgcatt gagaccgcca ttgagcttgc gcaggaaatc
     8521 cagcctcatg aaatagcggg aagcatcata ctagtgcatc ctgtcaatac acaggggttt
     8581 aacgcccgcg ggtcggacat tgttcccgaa gacgggaaaa atatcaaccg ggtgtttccg
     8641 ggcgacaggg acggaacgat taccgatcag attgcctatg ttttaactca tgaatttcag
     8701 gacgaggcgg atttttacat tgacattcac ggcggtgatc tgaatgagct tatgacacct
     8761 ctcgcctttt tccccggggc ggcagaacgg gaagttacgg aagtgtcact gggggccgcc
     8821 aaatcgcttc gtgtcgaata tcttgtcagg tcgggcgcga aaacgggagc ttacaattcc
     8881 gccgcaatcc gcggcgtgcc ttccattttg atcgagcggg gaggccgggg tctctggggc
     8941 agggaggaag tggaggaata caagaaagac gtccggaacg tgttaaagca tttgggtgtt
     9001 ttgaatggag agccggaatt gaacgggaaa actccggttg aagttaccgg cgtggtttat
     9061 ctcagggcga caaatgccgg ctgctggtat ccctgtgtga ctgcgaatga aaaggtcgcc
     9121 gtcggccaga agctgggcga ggttcgtgac tttttcggca aagttcttg
//
LOCUS       DATALY010000067         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_188443,
            whole genome shotgun sequence.
ACCESSION   DATALY010000067 DATALY010000000
VERSION     DATALY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188443"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHR42_02095"
     CDS             complement(<1..186)
                     /locus_tag="VHR42_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026009.1"
                     /translation="MKKINRALIYILGLFSLALGIVLNTKAKLGVSPIISIPYGISII
                     WNINPIMIVMITLDASST"
     gene            670..2010
                     /locus_tag="VHR42_02100"
     CDS             670..2010
                     /locus_tag="VHR42_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002126688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3026010.1"
                     /translation="MELSFKGLTGAVVTPYEEGYEESRQEWNRAIQKFPLAIVYCYSA
                     AEIQNAVCWARRNDVGIRIRSGGHHYQGYSVGNGVLVIDVSFRKHIELDKADGTVFMQ
                     SGIQNRDVYDYLGARGYPFPGGTCPTVGVAGYTMGGGWGYSSRYMGLGCDSLTGLEMV
                     DYQGNLIRADSSRNPDLLWACKGAGGGNFGVVTSMTFQLPPKMEQVTLLGLEYLDASP
                     SAMAEFLNTWQKWLIGLDPRITVNASLYHSAEDGKGIYGKGLFYGSASEAAQILKPFE
                     RIGVTISLAEMTFSEAIKTIQDAYPDSEKFQSTGRFVNRNYSMEEIETIVDLIRQRAE
                     GSVYTAVSVYALGGKVSEINQRDTAFYYRNSAYIMGIQSVWEEDRYASANRYWLNERF
                     QYLKQLTVGSYVNFPYNCLTDYEEEYYGGNVPRLQKIARRYDPINVFSFPQAIQ"
     gene            complement(2078..2794)
                     /locus_tag="VHR42_02105"
     CDS             complement(2078..2794)
                     /locus_tag="VHR42_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX3026011.1"
                     /translation="MKDSLCIPSDSADRSIQAFNLNAHFIAPNPPTLINPKSICPCIH
                     STAYVGPFSSVIGDVTIGENVFIAPNVSIRADEGTPFYIGSNTNIQDGVILHGLEYGR
                     VLHNGTEYSIYIGNEVSCTHGCIIHGPCKLEDHVFIGFHSIVLNAVIGEGSYISTNAL
                     VTGGIRIGRNRYVPSGAIIETQEQADALGPVPESQEEFAEEVQHVNQQFPNAYSFQFG
                     ATRCSCGLACDCHTLKAILD"
     gene            complement(2954..3823)
                     /locus_tag="VHR42_02110"
     CDS             complement(2954..3823)
                     /locus_tag="VHR42_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3026012.1"
                     /translation="MNEKIEAVQRMQDYIEAHLCETITLSDLSRVSLFSPWYSYRLFT
                     RWTDSTPSDYIRRLRLSKSALKLRDQSCKVADVAFELGFGSVDGYQRAFFREFGCNPR
                     DYAQKPVPLYLFTPYGVRAQNTVKEKKMENVKSIFVQIVEKPARKVLIKRGVKASDYF
                     SYCAEVGCDLWGLLTSIKSISGEPVCLWLPAAYIAPGTSEYVQGVEVPVSYDEAVPNG
                     FDVIELPAAKYLMFRGEPFQEEDYCQAIEEVWAAIDKYDPSAIGFSWDESNPRIQLEP
                     IGTRGYIELRPIQ"
BASE COUNT         1003 a          912 c         1006 g         1034 t
ORIGIN      
        1 tgtactggat gcgtcaagcg ttatcattac gatcataatc ggattgatat tccaaataat
       61 cgatatgccg tatgggatcg atataatcgg ggatacgccg agtttcgcct tggtgtttaa
      121 gacaattcct aacgcaagag aaaaaagacc taagatgtaa atgagagcgc gatttatttt
      181 tttcatgttt atttccttat cctattgtgt gattgttgcg gcgacgtccg gctatcacgg
      241 gagagctaaa tggttcagat acgcggaatg tttcaaaagc gttcattctc ctttatcaaa
      301 ttcttgtttg ccttgttccg gcggcaacga atgcctgttt ctattttact cttttatagc
      361 gaaaaagccc agcgttcgct ggacttttcc cggcagaccg ggggagtcga tcaaaatacg
      421 gcgccattta cttttccacc gcagtttcat tcgcgacatc tgaatcttag ttgtccggag
      481 gtaaggagaa ggaaaaagaa gtgcgcggaa ttaaaaactg aaattccaaa acggaacgcc
      541 gatcagttat tttccatttc aaccacttca tgaccagcgg agcgatcttc tgccgatgta
      601 acaaagcaga gagaagtcat attatggaaa gacagcattc agcaaatttt taagtttggt
      661 gatgaaaaaa tggaattaag ttttaagggc ctgaccggcg cggtcgttac cccttatgag
      721 gaaggatatg aagaatcgag gcaggaatgg aatcgggcaa ttcaaaaatt tccgcttgcg
      781 attgtgtatt gctattccgc tgcggaaatt cagaacgcgg tttgctgggc aaggcgcaac
      841 gatgttggaa tccgtattcg gtccggagga caccattatc agggctattc ggttggaaac
      901 ggcgttttgg tgatcgatgt cagtttccgg aagcacatcg aacttgataa ggcggacggc
      961 actgttttta tgcagagcgg gattcagaac cgtgacgttt acgattattt aggggccaga
     1021 ggatatcctt tccccggagg aacctgtcca accgttgggg ttgccggata cacgatgggc
     1081 ggcggctggg gatactccag ccgttatatg ggcctggggt gcgacagcct gaccggattg
     1141 gaaatggtgg attatcaggg aaatctcatt cgggctgact ccagccgcaa tcccgacctg
     1201 ctctgggcct gcaaaggggc gggtgggggt aatttcggcg tagtgacctc tatgacgttt
     1261 cagttgccgc ccaaaatgga acaggttacg cttttgggac ttgaatattt ggatgcttcg
     1321 ccatccgcaa tggcagaatt tctaaatacc tggcagaaat ggctgatcgg tttggacccc
     1381 agaataacgg ttaacgcaag cctgtatcat tctgcggaag acggcaaagg catatatggg
     1441 aaaggactgt tttatgggtc cgcctcagaa gctgctcaaa ttttaaagcc ctttgaacgc
     1501 atcggtgtaa ccataagcct ggcggaaatg accttttcgg aagcgatcaa aacgatccaa
     1561 gacgcctatc cggattccga aaagtttcag tccaccggca gatttgtcaa tcgaaactac
     1621 agcatggaag aaatcgaaac cattgttgat ttgatccggc agagagcgga aggttcggtc
     1681 tataccgcgg tgagcgttta tgccctcgga ggaaaagtca gcgagatcaa tcagcgggat
     1741 acggcgtttt attaccggaa ttccgcttat attatgggga ttcagtctgt gtgggaagag
     1801 gaccgatacg catccgccaa ccgatactgg ctcaatgaaa gatttcagta tttgaaacaa
     1861 ctgacggtgg gttcgtatgt caatttcccc tacaattgct taaccgacta tgaagaagaa
     1921 tattacggtg ggaacgtacc ccggcttcag aaaattgcaa ggcgatatga tcctataaat
     1981 gttttttcct ttccgcaggc tattcagtag catttagccg gttcctccca ttctgcaaaa
     2041 agctctccgt gatgttgaat caggagagct ttttcggtta gtctaaaata gcttttaagg
     2101 tgtggcagtc gcacgcaagg ccgcagctgc atctggttgc gccgaactga aatgaatagg
     2161 cgtttgggaa ttgctgattg acatgctgaa cttcttcggc aaattcctcc tgactttcgg
     2221 gcactggccc cagggcatcg gcctgctcct gcgtttctat aatagccccg gatgggacat
     2281 agcggtttcg gccgatccgg attcctccgg ttaccagcgc gttggtcgaa atgtagctgc
     2341 cttctccgat tacagcattg agcacaatgg aatgaaagcc gatgaaaaca tggtcttcca
     2401 gcttgcacgg tccgtgaata atacagccgt gtgtgcagct gacttcattc ccgatgtaga
     2461 tggaatactc ggttccgttg tgaaggactc ttccgtattc caacccatgc agaattacgc
     2521 catcctgaat attggtgttg gagcctatat aaaacggagt tccttcatct gcgcgtatgc
     2581 tgacattggg agcgataaaa acattttccc caatggttac atccccgatc acagaagaga
     2641 atggtcccac ataagcagta gagtgaatgc acgggcagat gctttttgga tttatcagcg
     2701 taggcgggtt cggtgcgata aaatgggcgt tcagattgaa tgcctgaatg gatctgtcgg
     2761 cagagtcact ggggatgcag agagaatctt tcatttgttt tcctcccggc cgttcggcct
     2821 cgaatcatgc ggcaagacga agaatatagt gtagaatatg aaataaagag gaaaaatgta
     2881 ccgaagagat tctgaaagac aaaataaact aagaattatc ttctccgaac tctgtagatt
     2941 ttcaggggcg ctgttattga atcgggcgca gttctatata gcctcttgtt ccgatcggtt
     3001 ccagctgaat tctgggattg gattcatccc acgaaaagcc gatcgcggac ggatcgtatt
     3061 tgtcgattgc cgcccagact tcttcgatgg cctgacagta atcttcctct tgaaacggtt
     3121 ctccccggaa catcaggtat ttcgccgcgg gcagttcaat cacgtcgaac ccgtttggta
     3181 ccgcttcatc ataggagacg gggacctcca cgccctgcac atactccgaa gtacccggag
     3241 caatgtaagc cgccggaagc cacagacata caggctcgcc gctgattgat ttgatgctgg
     3301 tcagcagtcc ccagaggtca cagccgactt ccgcgcaata ggaaaaataa tcggatgctt
     3361 ttactcctct cttgatcagc accttgcgtg ccggtttttc tacaatctga acgaatatgc
     3421 tctttacgtt ttccatcttc ttctccttta ccgtgttttg agccctgact ccatagggcg
     3481 tgaacagata cagggggaca ggcttctggg cgtaatcgcg ggggttgcag ccgaactccc
     3541 ggaaaaaggc ccgttggtac ccgtccacac tgccgaaacc aagctcaaac gcaacgtctg
     3601 cgactttgca ggattggtcc cggagcttca gcgccgattt tgacaatcgc agtcgccgga
     3661 tgtagtcgct tggggttgaa tctgtccacc gtgtaaacag gcggtaagaa taccatggcg
     3721 aaaacaagga gacccgcgac agatcggaaa gcgtaatcgt ttcgcacaaa tgcgcttcga
     3781 tatagtcctg catgcgctgt accgcctcga ttttctcatt catccgttat cacctcctca
     3841 cagtccttat tctatcaaca tcgaaacgaa ttctctcgat gattcttgct aagatgccgc
     3901 atgcccgtat tttggctgga tcaagaaaga aaatgggctg gcttcctgct ttgaa
//
LOCUS       DATALY010000068         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_620365,
            whole genome shotgun sequence.
ACCESSION   DATALY010000068 DATALY010000000
VERSION     DATALY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620365"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHR42_02115"
     CDS             <1..222
                     /locus_tag="VHR42_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX3026013.1"
                     /translation="FDVPSYCMTVTYQKRKRSNRPGTTIYLDGPFYPEKSLSKMKRKQ
                     KLRDDIYHCMERNSLNSTYSYIQYRKETG"
     gene            224..1102
                     /locus_tag="VHR42_02120"
     CDS             224..1102
                     /locus_tag="VHR42_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005711819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026014.1"
                     /translation="MNILYCGNDKTEDGLLISILSLLKNAGETLNIYLLTMRLNFGDM
                     VYSPISGSFAAYADRLAKQTNPANSVVLLDATNLFYSELPLMNLDTRFTPYCMLRMFA
                     DEIPELPERILYLDCDVICRKSCAEFYHQDISNYEFVGVLDHYGRWFFRRNFLHFDYQ
                     NSGVLLMNLRKIKETGLLSRCRRLCMVKKMFMPDQSALNKLAVSKKALPRRFNEQRSL
                     HKDTVLQHFTTSFRLFPWFHVMSVKPWEVEKVHRLLKLNEYDDLLAEYTHLLPEIKAK
                     KQENFNCRGDKNEQAE"
     gene            1086..2048
                     /locus_tag="VHR42_02125"
     CDS             1086..2048
                     /locus_tag="VHR42_02125"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEX3026015.1"
                     /translation="MNRRNEIIPIFFSVDDGYAPYLSVALTSIIRNASKKYSYKAVVL
                     YKELSVQNQKKLAALSTENFEIQFVSMGNRLDSITNQSCNRLRCDYFTLTIFFRLFIP
                     IMFPEFDKGIYLDSDIVVPGDISELFHTDMGENLIGAAADHSVVDVPQLAKYMEYGVG
                     VEKHHYINSGVLLMNLRRLREVQLERRFLELLDTYHFECIAPDQDYLNVLCSGRICYL
                     SPCWDAMPIKDAEPMKEPKLIHYNLFAKPWYYDQIPYESYFWEYARDCGYLDEIISCK
                     SNYDEEKQKTDTESMNRLLSTAEKVADSEINFRTVFQSGKERRL"
     gene            2045..2908
                     /locus_tag="VHR42_02130"
     CDS             2045..2908
                     /locus_tag="VHR42_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017262438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3026016.1"
                     /translation="MILGQKREQVIRNIRRAAESGSFYSKVEIDDPVLTEEQKKTILE
                     TYFSKKKTWRWQVNNRIARFAVNTASKKLNTETIMNGLEHLSGVTGGAIVTSNHFNPL
                     DNTLLRTLALKTGKNKLCIASEETNLAMSGWIGYLMNYADTVPVSSNLNYMGDKFDPM
                     LEGLLKQENWILIYPEQEMWFNYRKPRPLKRGAYYYAAKYRVPVISCFAEIHDLEQLD
                     TPEFHKVRYTLHVLPTIFPDPAKGIRENSIAMCQQDYRQKTAAYEAAYGKLLTYHFEP
                     SDIAGWIPSPI"
BASE COUNT          817 a          706 c          706 g          716 t
ORIGIN      
        1 tttgacgtgc cgtcctactg catgacggtc acctatcaga aacggaaacg ctcgaacaga
       61 ccgggcacca caatatatct ggatgggccg ttttatccgg agaaatctct ttcaaagatg
      121 aagcggaagc aaaagctgcg ggacgatatc taccattgca tggaacgaaa tagcctcaac
      181 agtacgtatt cctatatcca atatcggaag gagacaggat gaaatgaata ttctgtactg
      241 cggaaacgat aaaaccgaag acggtctgtt gatttccatc ctgtctttat tgaaaaacgc
      301 cggagaaaca ctgaacatct atttgctgac catgcgtctc aatttcggcg atatggttta
      361 ttccccgatc tcaggttcct ttgcggcata cgcggaccgt ctcgcgaaac aaaccaatcc
      421 cgctaattcc gttgtcctgc tggacgcgac aaatctgttt tacagcgagc tccccctcat
      481 gaatctggat acacgtttta ctccttactg tatgctgcgg atgttcgcgg atgaaattcc
      541 cgagctgccg gagagaatcc tctatctgga ctgcgacgtg atctgccgaa aaagctgtgc
      601 ggagttttat catcaggata tctccaacta tgaatttgtc ggagttctcg atcactacgg
      661 acgctggttc ttccgccgaa atttcctgca tttcgattat cagaattccg gggttctgct
      721 gatgaatttg aggaagatca aagaaaccgg gcttctcagc agatgcagga ggctctgcat
      781 ggtaaagaaa atgtttatgc cggaccagtc cgcgctgaac aagcttgctg tttctaaaaa
      841 agccctgccg cgtcggttca acgagcagcg cagcctgcac aaggatacgg ttctgcagca
      901 ctttacgacc agctttcgcc ttttcccatg gttccatgtt atgtcggtca aaccctggga
      961 ggttgaaaag gtacaccgtt tgctgaaact taatgaatat gacgatttgc ttgctgaata
     1021 cacgcatctg ctgccggaaa taaaggcgaa aaaacaggag aattttaact gcagggggga
     1081 caaaaatgaa caggcggaat gaaatcatcc ccattttctt ttccgttgac gacggctatg
     1141 cgccatactt aagcgttgcg ctgacctcga tcatccgcaa cgcttcaaaa aaatacagtt
     1201 acaaagcggt agtgctttac aaggaactca gcgtccaaaa tcaaaagaaa cttgccgctc
     1261 tttcaaccga gaatttcgaa attcagtttg tgtcgatggg gaaccggctc gacagcatta
     1321 caaaccagtc ttgtaacagg cttcggtgcg actattttac gctcacgatc ttttttcgcc
     1381 tgtttattcc cataatgttt ccggaattcg acaaggggat ttatcttgac agcgatatcg
     1441 tcgttccggg ggatatttcg gaactgttcc acacggacat gggagagaat ctgattggtg
     1501 cagcggcgga ccactcggtg gtggacgtgc cgcagcttgc aaaatacatg gaatacggcg
     1561 tcggcgtcga gaaacatcac tatatcaatt cgggtgttct gctgatgaac ctcaggcggc
     1621 tgcgggaagt acagctcgaa cggcgatttc tggagctgct cgacacctat cattttgagt
     1681 gcatcgcacc cgatcaggat tacctcaacg tgctttgcag cggtagaatc tgctaccttt
     1741 ccccctgctg ggatgccatg ccgatcaaag acgcggaacc aatgaaagag ccgaagctga
     1801 ttcactataa cctgtttgcc aagccctggt actacgacca aataccgtat gaatcgtact
     1861 tttgggagta tgcccgcgac tgcggatatc tggatgagat tatcagctgc aagtcgaact
     1921 acgatgaaga gaagcaaaaa accgatacgg aaagcatgaa ccgtttgctt tccaccgctg
     1981 aaaaggtagc ggattcggag attaactttc ggaccgtatt tcagtcgggg aaagagcggc
     2041 ggctatgata ttgggccaaa aaagggaaca ggtaattcgg aatatccgcc gcgcggcgga
     2101 aagcggctcc ttctacagca aagtggaaat cgatgatccg gtgcttacgg aagaacagaa
     2161 aaaaacgatc cttgaaactt atttttcgaa aaaaaagacc tggcgctggc aggtgaataa
     2221 ccgaattgca cggtttgcag tcaacaccgc cagtaaaaaa ctaaatacag aaacaatcat
     2281 gaacgggctt gagcatcttt ccggcgtcac tggcggcgcg attgtaacca gcaatcattt
     2341 taatcccctt gataatactc tgctgcgcac cctggcatta aaaaccggaa aaaacaagct
     2401 gtgtatcgca agcgaagaaa cgaaccttgc catgtccggc tggatcggct acctgatgaa
     2461 ctatgcggat accgttccgg tcagttccaa tctgaactac atgggagaca agtttgaccc
     2521 aatgctggag ggtctgctga agcaggaaaa ctggattctg atctatccgg aacaagaaat
     2581 gtggtttaac taccgaaaac cccggcctct gaaacgtggg gcttattatt atgcggcaaa
     2641 ataccgagtc cccgttattt cctgttttgc cgaaatccac gacctggaac aattggacac
     2701 gccggaattt cacaaggtac ggtatacgct gcacgtgttg ccgactattt tcccggaccc
     2761 ggctaaaggc atacgggaaa acagcatcgc catgtgtcag caggattatc ggcagaaaac
     2821 ggccgcttat gaggcagctt acggaaaatt acttacctat cactttgaac caagcgatat
     2881 tgcagggtgg attccaagcc caatctgacc taaatgttca aattagctaa ttcctttttc
     2941 ataat
//
LOCUS       DATALY010000069         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_361293,
            whole genome shotgun sequence.
ACCESSION   DATALY010000069 DATALY010000000
VERSION     DATALY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361293"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..78)
                     /locus_tag="VHR42_02135"
     CDS             complement(<1..78)
                     /locus_tag="VHR42_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX3026017.1"
                     /translation="MRIDILTLFPEMCESVLNESIVGRAR"
     gene            complement(68..583)
                     /gene="rimM"
                     /locus_tag="VHR42_02140"
     CDS             complement(68..583)
                     /gene="rimM"
                     /locus_tag="VHR42_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485931.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX3026018.1"
                     /translation="MLKRFLETGQIVTTHGLKGEVRVIPWSDTPDFLLAFDRFFFDRG
                     DRYMEVESSRVHKSLVIIKFSGVDSLEQAMPLLRQTLYIDRSWVELPEGSYFEQDLIG
                     LKVIHEQTGKEYGQITAVSKTGANDVYHILRSEGGEVLIPAIPDVIRSVDLDGAEMKI
                     IPLKGLFSDED"
     gene            complement(691..924)
                     /locus_tag="VHR42_02145"
     CDS             complement(691..924)
                     /locus_tag="VHR42_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX3026019.1"
                     /translation="MQDLLIAIVQGLVENPSEVKVTEDPPTDDGTIVYHLHVAEGDMG
                     RVIGKQGRIAKAIRTVVRAAANRQNKKIAVEID"
     gene            complement(1067..1309)
                     /gene="rpsP"
                     /locus_tag="VHR42_02150"
     CDS             complement(1067..1309)
                     /gene="rpsP"
                     /locus_tag="VHR42_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX3026020.1"
                     /translation="MAVKIRLRRMGKKKTPFYRIVVADSRFPRNGRFIEEIGYYNPLE
                     NPPVVKVDSEKAKKWIENGAQPTDTVKALLKKNSEL"
     gene            complement(1355..>2509)
                     /gene="ffh"
                     /locus_tag="VHR42_02155"
     CDS             complement(1355..>2509)
                     /gene="ffh"
                     /locus_tag="VHR42_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX3026021.1"
                     /translation="TERAVGVEVLESLTPAQQVIKIVNEELTGLMGGEQSKINFSSHP
                     PTVVMLCGLQGSGKTTHAAKLASLFKKQGKRPLLVACDVYRPAAIKQLQVVGEKAGVP
                     VFEMGTGNPVKIAKAAVSNAKDHGNDLVILDTAGRLHVDQELMDELRNIKAEVSPNEI
                     LLVVDAMTGQDAVNVASSFNETLGIDGVILTKLDGDTRGGAALSVRAVTGKPIKLVGM
                     GEKLDDLEPFHPGRMASRILGMGDMLTLIEKAQQQVDEKKAAQLAKKMKKNSLDLEDF
                     LEQMQQIKKMGSLNSLLDMVPGMGKQLKDVEIDDKQIAHVEAIITSMTPKERQKPETV
                     NPSRKRRIAAGSGMKVEDVNRLLKQFEQSRKMMKQMSNMSKSKRRFMLPF"
BASE COUNT          570 a          710 c          550 g          679 t
ORIGIN      
        1 tctggctcgc ccgacaatgc tctcgtttaa aaccgactcg cacatttccg ggaaaagcgt
       61 cagtatgtca atcctcatcg ctgaacagtc cttttaatgg aataattttc atctccgcac
      121 catccaaatc aaccgaacgg atgacgtctg gaattgccgg aatcagaacc tcgccgcctt
      181 cggacctcaa aatatggtag acgtcgttgg ctccggtctt gctgacagcg gtgatttgac
      241 cgtattcttt tccggtttgc tcatgaatca ccttcaaccc gatcagatcc tgctcaaaat
      301 agcttccttc cggcaattcc acccaactcc ggtcaatata cagtgtttgt ctgagcagcg
      361 gcatggcttg ctcaagcgag tcaacaccgc tgaattttat aatgacaagg cttttatgaa
      421 ccctggaact ctcaacttcc atgtatctgt ctccccggtc aaaaaagaag cggtcaaatg
      481 caagcagaaa atccggggtg tcgctccacg gtatgacgcg aacctcacct ttaagaccgt
      541 gagtcgttac aatttgtccc gtttctaaaa accgttttaa cataatacct ccgcatcgtt
      601 tgcccggcta tccgccggta tttttaagct accctgaaaa ccttaattta tgaaaaaagg
      661 gaaagcctcc ggccttccca gtttccacaa tcagtcaatt tctacggcaa tctttttgtt
      721 ttgccggttc gctgcggcac ggacaacagt tcgaatggct tttgcgattc ttccctgttt
      781 tccaatgacg cgacccatat caccctcggc aacgtgaaga tgatagacaa ttgtcccatc
      841 gtccgtcggc ggatcctcgg tgactttgac ttcagaagga ttctccacaa gaccctgcac
      901 aatggcaatt aacaaatcct gcattttaat ttccattcag ccgctgccgt tgcggcaaag
      961 cagagaatcg cagctttccc ccggcgggtt tggaaaagcc gcgttgtcat ggagccggtc
     1021 aaggattctc atacgggaac cccggccgga tccgtttcat cgtaaattag agttcgctgt
     1081 ttttcttaag gagcgccttc acggtgtccg tcggctgagc tccgttttct atccattttt
     1141 tcgctttttc actatcaacc ttgacaacag ggggattctc gaggggattg taataaccga
     1201 tctcctcaat aaaacggcca ttgcggggaa atcttgaatc ggcgacaaca attcgataga
     1261 aaggagtctt ctttttaccc atacggcgaa gcctgatttt tactgccatg catttcacct
     1321 cccacattag taatcggccc atacggccat tcggttaaaa cggcagcata aaacgccgtt
     1381 tcgatttcga catgttactc atttgcttca tcatcttgcg gctctgttca aactgcttga
     1441 gcaggcggtt gacatcctcg accttcatac cgctgccggc ggcgatgcgc ctttttcggc
     1501 ttggattaac cgtctcgggt ttctggcgct ccttcggagt catggatgtt atgatcgcct
     1561 ctacgtgggc gatctgcttg tcgtcaatct ccacatcctt tagctgtttg cccatccccg
     1621 gaaccatatc aagaaggctg ttgagcgagc ccatcttctt aatctgctgc atctgctcaa
     1681 gaaagtcttc cagatccagg ctgtttttct tcattttttt tgcaagctgg gctgcttttt
     1741 tttcatccac ctgctgctgg gctttttcaa tcagcgtgag catgtcgccc ataccaagaa
     1801 tccgtgaagc catccgaccc ggatgaaaag gttcgagatc gtcaagcttc tcgcccatac
     1861 ccaccagctt aatcggcttc cccgtcacag cccggacgga gagtgccgcg ccgcctctgg
     1921 tatcaccgtc aagctttgtc agaatgacgc cgtcgatgcc cagtgtttcg ttgaaagacg
     1981 aggccacgtt gacggcatcc tgaccggtca ttgcgtcgac cacaagaaga atctcattcg
     2041 ggctgacttc agctttgata tttctcagct catccatcag ttcctgatcg acatgaagac
     2101 ggccggcggt atctaaaatc accaagtcgt ttccgtgatc ctttgcattg gaaacggccg
     2161 ctttcgcaat tttcacagga tttccggtac ccatttcaaa aacaggaact ccggcttttt
     2221 cccccactac ctgaagctgt ttgatcgccg caggccgata aacgtcgcat gccaccagca
     2281 acgggcgctt gccctgcttt ttaaacagag atgcaagctt ggccgcgtgg gtagttttac
     2341 cggatccctg aagaccacac agcatgacaa ccgtgggcgg atgagaggaa aaattgattt
     2401 tactctgctc cccgcccatc aggccggtga gctcctcatt tacaattttg atgacctgct
     2461 gggcaggcgt cagactctcc agaacttcca cacccactgc acgctcggt
//
LOCUS       DATALY010000070         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_792855,
            whole genome shotgun sequence.
ACCESSION   DATALY010000070 DATALY010000000
VERSION     DATALY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792855"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VHR42_02160"
     CDS             <1..216
                     /locus_tag="VHR42_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026022.1"
                     /translation="YLSTSVKGNKEVLASAFVKKGQELGKRLTPVLKRQRYPSGIMES
                     IFGILAIVCVGVVLYRLYASRQTDSLS"
     gene            complement(420..1352)
                     /gene="cysK"
                     /locus_tag="VHR42_02165"
     CDS             complement(420..1352)
                     /gene="cysK"
                     /locus_tag="VHR42_02165"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX3026023.1"
                     /translation="MTKIAKSLTDLIGNTPLLELSNYNRSHGIETALIAKLEYFNPGG
                     SVKDRIGLAMIKDAEEKGLINHETTIIEPTSGNTGIALALVAASRGYRLIITLPETFS
                     VERRKLMKALGAELVLTPGAEGMKGAIKKAEDLAAETKNSFIPQQFKNPANPAIHRTT
                     TAEEIWRDTDGKIDIFVAGAGTGGTVSGVGQALKKKNPKVKIVAVEPADSPVLSGGKP
                     GPHKIQGIGPGFVPEIFDRDVVDEIITVQNEDAFEASRAIARQEGLLVGISAGAAVSA
                     ATQLAKRPENKGKKIVVLLPDTGERYLSTLLFQE"
     gene            complement(1527..>2766)
                     /locus_tag="VHR42_02170"
     CDS             complement(1527..>2766)
                     /locus_tag="VHR42_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX3026024.1"
                     /translation="ICCGCGACAGICCRIRMTEDRLGEYSPQFSGPCGGSGKCAEVCP
                     MNADSRCSLTAFGEWGEKKEESIGQYADCYTVPAREEQKRENSAALAGLFRLLLSSGE
                     ADSVIYPARTEDPAKFYDYRICTDPDLLRACPGFFCFPLEISGVLRYIKNKPGKYAVA
                     ALPCGVKALRLAMMLDVVIAQRITYIIGISCLQNKSRGWLEYCSEQSRFGRDLISAEL
                     LRRTSESGEALYEYSFVYSDGSKRFVPFPKAAWKNRIHTLKGCGSCDDLFAEDADVTL
                     IGDNPVFGIRDEMLIVRSNRIQKLVTAPESKMGLVKIEASALLEKTASQIAFDRASVF
                     YRPERTRRRLAAFRSGNPPGFKTGLLEAVRQRLAYSSGDRRRKKALRKLTRGEEISRV
                     RCWQDGLLHFISRWQEHRNH"
BASE COUNT          589 a          731 c          712 g          734 t
ORIGIN      
        1 tatctgtcga catccgtgaa aggaaacaag gaggttcttg cttctgcatt cgtaaaaaag
       61 ggacaagaac tcggcaaacg cctgactcct gttttaaaac ggcagcgtta cccgagcggt
      121 attatggaaa gcatttttgg aatactcgcg atcgtttgtg tgggggtagt gctctaccgg
      181 ctctacgcat cgcgtcagac cgacagcctc tcctgatagg tgccgggtat tttcagcccc
      241 atggacggaa gtccacgggg ctttcggatg tcatgggtag aatctccata aatcaagggc
      301 cgtcgtctcg gtttggacgc cggctttctt tttgtgcttt taaattttgt aaataaagta
      361 agcctcacga ctaccgtgag gcttacgtta ttttaagtca tcgaaaatat tgttttgcct
      421 tattcctgga aaaggagtgt ggaaagatat ctttctcccg tgtcgggcag cagaacgaca
      481 atttttttac ccttgttttc cggccttttt gccagctgag ttgccgcgct gacagcggct
      541 ccggcggata ttccgaccag gagaccttcc tgtctggcga tagccctgga tgcttcgaac
      601 gcgtcttcat tttgaaccgt gatgatttcg tccactacgt cacggtcaaa gatttccgga
      661 acaaatcccg gcccgatgcc ctgaatttta tgcggcccgg gttttcctcc ggagagcacc
      721 ggtgaatcgg caggctcaac agcgacaatc ttcaccttcg ggttcttctt tttgagcgcc
      781 tggcccacgc cggaaaccgt tccgcctgtg cctgcgccgg caacaaagat gtcgattttc
      841 ccgtcggtat cgcgccagat ttcttcggcg gtggtcgttc tgtgaatggc gggattggcg
      901 gggtttttaa actgctgggg aataaacgag tttttggttt cggcggcaag atcttccgcc
      961 ttcttgattg cgcctttcat tccctccgca cccggggtca gaacaagctc cgctcccaga
     1021 gctttcatca gcttacggcg ttcaacgctg aaggtctctg gcagggtgat gatcagccgg
     1081 tatcctctgg aagccgcaac gagggccagc gcgatgcctg tgttgccgct ggtaggctca
     1141 atgatggtcg tttcatggtt gatcaggcct ttttcttccg catccttgat catcgcaagg
     1201 ccgatccggt ctttcacgct tcctccggga ttaaaatatt ccagctttgc aatcaaagcg
     1261 gtttcgatcc cgtgagaacg gttatagttg gagagctcca gaagcggggt gttgccgatt
     1321 aaatccgtca ggctttttgc aatctttgtc atgaggaatc ctccctttgt agtataagta
     1381 atcatattga ttttgtatgt attaatcata gaacaaaaag tctctgttgt caagagactt
     1441 ttgataaatt tattattatt tatagcggct taatatataa tcatcttttg tgacttagtt
     1501 ccatgccggc gggcgaagcg ttttaatcag tgattcctgt gctcctgcca tcgtgaaatg
     1561 aaatgaagca gcccgtcctg ccagcagcga acccggctga tttcttcccc gcgcgtgagt
     1621 ttacggaggg ctttctttct tctgcggtcg ccggaggaat aggccagtct ttgccgcacc
     1681 gcttccaaaa ggcctgtttt aaatcccggc ggattgccgg atcggaaagc agccagccga
     1741 cgtctcgttc tttccggacg gtaaaaaacg gacgcacggt caaatgcaat ttgcgaagct
     1801 gttttttcca gaagggcgga ggcttcaatc ttcacaagac ccattttgct ttcaggggcc
     1861 gtcacaagct tttgaatccg gttcgaccga acgatcagca tctcatcccg tatgccgaat
     1921 acgggattat ctccgatgag agtcacatcc gcgtcttccg caaacaggtc gtcgcaggag
     1981 ccgcatccct tcagcgtatg gattctgttt ttccaagccg ctttcggaaa cggaacgaac
     2041 ctcttcgagc catccgaata aacaaacgaa tactcataca gcgcttctcc gctttcggac
     2101 gtgcggcgaa ggagttcggc gctgatcaaa tcccggccga atcttgactg ttccgagcag
     2161 tattccagcc agcctctgct tttgttctgc aggcagctga taccaatgat ataggtgatt
     2221 ctctgtgcaa tgacaacatc cagcatcatg gcaagcctca gcgccttgac gccgcagggc
     2281 agagctgcca ccgcgtattt tccgggcttg tttttgatat atcggagtac gccggagatc
     2341 tccagcggaa agcagaaaaa gcccgggcac gctctcagca gatcgggatc cgtgcagatc
     2401 ctgtaatcat agaatttagc cggatcttcc gttctggcgg ggtaaattac gctgtccgct
     2461 tcgccggaac tcagcagaag ccggaacaga ccggcgaggg cggcagagtt ttctcttttt
     2521 tgttcctccc gggccggaac ggtgtagcag tctgcatatt gcccgataga ttcctctttt
     2581 ttttcacccc actcgccgaa cgcggtcagg gaacaccggc tgtcggcatt catcgggcag
     2641 acttcagcac attttccgct gccgccgcag ggcccggaaa attgcggaga atattcgccg
     2701 agccggtctt ccgtcattcg gatgcggcag caaatacccg cgcaggcgcc gcagccgcag
     2761 cagatt
//
LOCUS       DATALY010000071         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_793225,
            whole genome shotgun sequence.
ACCESSION   DATALY010000071 DATALY010000000
VERSION     DATALY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793225"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1350)
                     /locus_tag="VHR42_02175"
     CDS             complement(280..1350)
                     /locus_tag="VHR42_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3026025.1"
                     /translation="MSNPIRVGLVGLGRAGWGMHLSELEGKEDKFRIVAACDRIPERN
                     ELVKKRCACRTYGSIEELIADPEVELVDIATRSCDHYEHACMALAVGKDVLLEKPAGT
                     SYQQFLDLLTRANRKGLPRLYFRQNRRFETAFQEVLSIIKSGILGNVFEVQLSEFEYQ
                     RRDDWQTLSKFGGGQILNWGPHLIDHALRFLDAPISRVDSNLILGAAGGDCEDHFSIY
                     ITSENGRYATVSVSGSAALNGERRYSAYGSRGALQMVNNRIKLRYIDPNQKLPEVVSD
                     PGTPANEFGATGTFKAANGPIWIEEERVAPEEDLSVFWGYLFDSYRNGKPFPIKDEEV
                     KELMWTISKAKERRIVKACAIR"
     gene            complement(1364..2104)
                     /locus_tag="VHR42_02180"
     CDS             complement(1364..2104)
                     /locus_tag="VHR42_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3026026.1"
                     /translation="MIKVAVADYGLYMWYGDIYDYEERLLSLKKLGYDGLERLYAKTE
                     AEAVNTSALARKLGMDFATCEGASPSQSLHWSAALCKKYVWAESTSMDFDVFCRQVNS
                     QIESARRYGVIVGLHNHLGSAVETQEQIVSYMNRCPKSGLILDVAHLAGAGGDPLEII
                     DAYPGKLVMVHLKDYVYLNRNADKWYERIRFCELGAGEMADTNRRIVEKLREKGYGGW
                     IAVEQDTHLRDPMLDLKESRGLLKEYGI"
BASE COUNT          546 a          642 c          491 g          690 t
ORIGIN      
        1 aaacaccgtc acgataatcg gacttatgct gacagtcggg ataaatcccc gttgtgggca
       61 gaaggaaaag cgcataccct ttttcgataa tatagtccag cggaacattc ggggaggaaa
      121 aagttccttc cagcgtcatt cgccatagtt ttcaaaaaga tttaaatttc ggtgcggcgg
      181 aatttctccc aaacatccac actgtaaacg ttttccccgc ttaaggtttt taatatttcc
      241 ggcattgcca taacagttcc tcctttttca attgcttttt cagcgaatcg cgcaagcttt
      301 aacaatccgt ctttccttgg ccttggaaat agtccacatc agttctttta cttcttcgtc
      361 ttttatggga aacggctttc cgtttctgta cgaatcgaac agataacccc aaaacacgct
      421 taaatcttcc tcgggagcga ctctttcttc ctcgatccaa atcggtccgt ttgcagcctt
      481 aaaagttccc gtcgcgccga attcgtttgc cggtgtgccc gggtcggaca caacctcggg
      541 aagcttttga tttgggtcga tgtagcgcag ttttatacgg ttgtttacca tctgcagtgc
      601 acctctgctt ccgtaagcgg aatagcgcct ttcgccgtta agtgccgccg aaccgctgac
      661 agaaacggta gcataacggc cgttttcgga ggttatgtag atggaaaaat ggtcttcgca
      721 gtcaccgcct gctgctccca aaatcaaatt cgaatccact ctgcttatcg gagcgtccag
      781 aaacctcaga gcatggtcaa tcaagtgcgg tccccagttc aggatttgcc ctccgccaaa
      841 cttggaaagc gtttgccagt cgtctcttct ctgatactca aattccgaga gttgaacctc
      901 aaacacattt ccgagtattc ccgattttat gatgctcagt acctcctgga acgcggtttc
      961 aaaacggcgg ttctgtctga aataaagtct gggaagccct tttctgttcg ctctggtaag
     1021 caaatctaga aattgctggt agcttgtgcc ggcgggtttt tcgagcagca catcttttcc
     1081 tactgccaaa gccatgcaag cgtgttcgta gtggtcgcag cttcgggttg caatgtcgac
     1141 aagttctacc tcggggtctg cgataagctc ctcaatcgag ccgtatgttc gacatgcaca
     1201 gcgctttttt accagctcat tgcgctccgg gatccgatca caggccgcaa caatcctaaa
     1261 cttatcttct tttccttcaa gctctgacaa gtgcattccc cagccggcac gcccaagtcc
     1321 caccaaacca acacgaatcg gattactcac ggtgaacccc tccttaaatt ccgtattctt
     1381 ttaataaccc acggctttct ttcaggtcga gcatcgggtc ccgcagatgt gtatcctgct
     1441 caaccgcaat ccagccgcca tatccttttt ccctcagctt ttcgacgatg cggcggttcg
     1501 tgtccgccat ttctcctgcg ccaagctcac agaaacgaat tctttcgtac catttatccg
     1561 catttctgtt caggtaaaca tagtctttca aatgaaccat aaccagtttg ccaggatacg
     1621 catcgatgat ctcgagcggg tctccaccgg ctccggccag gtgggcgaca tcgaggatca
     1681 atccgctttt ggggcagcgg ttcatatagc tcacaatctg ttcctgagtt tccaccgcgc
     1741 tgcccagatg attatgcagg ccgacgatca caccgtatct tcgtgctgac tcaatctgag
     1801 aattcacctg cctgcaaaaa acgtcaaaat ccatagacgt gctttctgcc caaacatact
     1861 ttttacaaag agctgcagac caatgcaggc tctgtgacgg acttgccccc tcacaggttg
     1921 cgaaatccat tccgagtttc cttgcaagag ccgatgtgtt tacagcctcg gcttccgttt
     1981 ttgcatacag tctttccagc ccgtcatatc cgagtttctt cagcgataaa agccgttcct
     2041 cgtagtcata gatatcaccg taccacatgt acaaaccata atcggctacc gctactttta
     2101 tcatggccgt ctttcctttc cgcccaaaat tttcaagggc acatctccgt ctgttttggc
     2161 agtccgttcc cttcctttgc attcacaata tcatttttgt cgctttcgga acattgacta
     2221 tgttgggaag atttcagctt ttttggtatt atattttgat tctttgctta acagcatttt
     2281 ttcagcgcat agattgactc tttggttttc agtggtagaa tgatggggat taaggaattc
     2341 caccatttcg ttcgtaatat ataacgatt
//
LOCUS       DATALY010000072        15271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_189718,
            whole genome shotgun sequence.
ACCESSION   DATALY010000072 DATALY010000000
VERSION     DATALY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15271
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189718"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1001
                     /locus_tag="VHR42_02185"
     CDS             255..1001
                     /locus_tag="VHR42_02185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX3026027.1"
                     /translation="MITKKPVFYITLTVTVIITVISVIFCVVLAKRKHAIGQINAGYN
                     SSAVTKKAKTVIIDPGHGGPDPGATGVGGVLEKNLNLIIAEKLKADFETEGYTVIMTR
                     NDDRSINDPGCISLYDKKNSDLNNRIKIGASHPQAIFISIHQNFNDNSNYTGTQVYYS
                     VNKPGSKLLAQDIQSEIRTDLQPSNSREIQEQGKLRVLLKAEIPAVLIECGFISNAND
                     THNLENSSYQERLDAAIVRATDTYIYNAAN"
     gene            complement(1291..2985)
                     /locus_tag="VHR42_02190"
     CDS             complement(1291..2985)
                     /locus_tag="VHR42_02190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX3026028.1"
                     /translation="MKFSQMEYKQVDFPSYQEKSQKILSQFRNSASAAEQVDSLGQFY
                     RLEDEYVTMSSLSYIRNSVNTADPFYAKEKEYYDENGPKFEALSMNFYKALVDSPYRA
                     ELTEKLGPLLFQNAEIALKSFDDRLIGDMAEENRLKTQYTNLIASASIKFAGKTLNLS
                     QLGYYMNSSERETRKSAYAARTEFFLKNERELDDIYDKLVKLRDKMAKKLGYKNYIEL
                     GYLRMMRNSYKPEDVAAFRQSVKEKIVPLTRRLHEKQRQRLGLDQLMYYDEKTLFPGG
                     NPLPHGTPEEIFQNGKKMYHEMSRQTGEFIDFMIGNGLFDVLGRANKAAGGYCIALPD
                     YQSPFIFANFNGTHDDINVLTHEAGHAFEFYLARDVYPAYREPTNDAAEIHSMSMEFF
                     CWPWTPLFFGNKAEQYHYLHLFEAIVFIPYGCAIDEFQHIVYQNPEMTPAERRQAWKK
                     LEEAYLPHLNFGGDPFFGNGGTFLRQLHVFEYPFYYIDYCLAQTCALEFWEMSRKEKS
                     AAWEKYLKLCRMAGTKVFNGLVAGAGLRSPFDPACVDSIAKTVEGWFDSFDESLLQ"
     gene            3323..5302
                     /gene="ligA"
                     /locus_tag="VHR42_02195"
     CDS             3323..5302
                     /gene="ligA"
                     /locus_tag="VHR42_02195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX3026029.1"
                     /translation="MESEKFLQRAEELRRLIQYHNLRYYVYDSPEIDDYEYDMLLREL
                     EQIEEEHPEIITPDSPTQRVGGQAVGLFGKVTHEVPMESLQDVFSKEEVLAFDQRVTP
                     AIGKAVYVVEPKIDGLSVSLEYENGVFIRGSTRGDGIVGEDVTSNLRTIRSIPLRLTR
                     PVPFLEVRGEVYMPRSSFLKLTEQQELNEEKPFKNPRNAAAGSLRQKNPKITASRGLD
                     IFIFNIQRIEGETVDTHSGGHELLKSLGFRIVPNYRICDAIETAAEEIDEIGQMRGKL
                     PFDIDGAVIKVNSLPARTRLGSTAKFPRWAVAYKFPPEEKETRLLDIEINVGRTGSLT
                     PTAVLEPVLLAGTTVGRATLHNQDFITEKKIKIGDVVVVRKAGDIIPEIVSVARDEGT
                     QPYLMPTVCPSCGQPVMQEQSEAVLRCTNAECPAQLIRHLIHFVSRDAMNIEGLGPAV
                     IEALSAKGLVKSPADLYDIKAEDLISMERMGEKSASNLINAIERSKTSDLSRLVYALG
                     IRNVGQKAAKLIAGRFRDMDRLFSAGVEELTAIDEIGEIIARSVVNFFALDSTAHLVE
                     RLRRAGVNMTEQSASGGEGRLKGMTFVLTGTLPGFTRSKAAEISEKLGGKVTGSVSKK
                     TSYVLAGEDAGSKLVKAQQLGVTVIDEKRFRELIQ"
     gene            5395..5673
                     /gene="gatC"
                     /locus_tag="VHR42_02200"
     CDS             5395..5673
                     /gene="gatC"
                     /locus_tag="VHR42_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX3026030.1"
                     /translation="MTEMNIDIRHIAKLARLHVDEAEIQKLGKEMAAIIGMVENLPEF
                     ENARLELSEKDAMKLREDEVRPSMSRKEVLKNAPETEAGCIVVPKIVE"
     gene            5701..7158
                     /gene="gatA"
                     /locus_tag="VHR42_02205"
     CDS             5701..7158
                     /gene="gatA"
                     /locus_tag="VHR42_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX3026031.1"
                     /translation="MELYEYTAAGLSKMLKEKKCGAREIAESVFSRIDSVEEKVGAYV
                     TLTSELALRQADETDRKIAAGAEIGPLAGIPVGIKDNLCTKDVLTTCSSKILGNFVPP
                     YDATVVRKLLDAGVVVTGKMNMDEFAMGSSCETSAMKKTHNPHDLTRVPGGSSGGSAA
                     AVAAGEAVLTLGSDTGGSIRQPASFCGVVGLKPTYSAVSRFGLVAFASSLDQIGPFAR
                     SVEDAAMLFDTIRGHDDYDATSSPLDHPSIADKLDGNVKGLKIGIPDEFFGEGVEDGI
                     RHTVLKAAKKLESLGAILMKISLPSTKFALPAYYIIACAEASSNLARFDGVKYGYRAE
                     KYENLIDMYERSRSEGFGPEVKRRIMLGTFVLSSGYYDAYYKKAKLTQNRIRSEYDDA
                     FQICDVILTPTSPGTAFRLGERADDPLKMYAADICTVPVNIAGLPALSLPCGMDAEGL
                     PIGLQLIGRKFGEQTILNAGHAYEKCGGGIAIPRI"
     gene            7170..8612
                     /gene="gatB"
                     /locus_tag="VHR42_02210"
     CDS             7170..8612
                     /gene="gatB"
                     /locus_tag="VHR42_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX3026032.1"
                     /translation="MNYETVVGLEVHAELSTNTKIYCSCKNAFGGEVNAQCCPICTGM
                     PGTLPTLNKKVVEYAVRMGYALNCGLNRICKQDRKNYFYPDLPKAYQISQFDVPLCEN
                     GYLDVWVDGQEKRIGVTRIHIEEDAGKLLHDESFSGSLVDFNRCGVPLIEIVSEPDMR
                     SSKEAKAYLETIRSILLALDISDCKMQEGSIRCDVNVSVRPQGSDTFGTRCEMKNVNS
                     FSGAVRAIEYETKRQIEIIEDGGVIVQETRRWDDVKGMSFTMRSKEDAQDYRYFPEPD
                     LKTIVLEEDDLRRMKESIPELPNKKLIRYMKDYKLPEFDANLLSENPEKSKFFESCLE
                     AAKVEPKTLSNWIVGDVTRILNEQGIGITESVLTPEKLTALIQLIDKGTISNTAGKTV
                     LDEMFATGGRPDDIVREKGLSQISDASALEELAKGVLADNQKSVDDYRGGKTNALGYL
                     VGQCMRASKGKGNPQTIREILLKVLQSEGS"
     gene            8706..9236
                     /locus_tag="VHR42_02215"
     CDS             8706..9236
                     /locus_tag="VHR42_02215"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX3026033.1"
                     /translation="MEDSALFTVSTVMEKEDYRAFMNLATFRKSPVIIPLLLLITAAI
                     ALIITVVCGAFSLLVFMICWLVELLTILISFYIKIENKIKKIEKTNSGATFGSRQTIN
                     FFDEYLVASNEGIEGSNKIKYEQFYQLMESKNYFLLYFNATMGALIRKKDIGPGELDS
                     LREHFRNKFGAKFRKI"
     gene            9335..10516
                     /locus_tag="VHR42_02220"
     CDS             9335..10516
                     /locus_tag="VHR42_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3026034.1"
                     /translation="MEFEKSGMKKWILLIAFGILFYLGMQNLYRIPDLSNYIGGIFEP
                     ILLGLALAFVLNVLMFQVESRLFAPLNRRYTKKWPKIRRPLSILITFVIVLGAITLIL
                     LIIVPELVRTITSLTNKIPVFFNDLQTGLTKISREYPGIGEYISNIKIDWTSISQMLA
                     QYGQQFAKLLVGSTVAVTTNVFHAAITSILGLVIAINILAQKEKLQNQLKRVLYAYLP
                     QKATSRFIRLCSLTNHVFSNFIAGQCTEACILGTLCFIGMQIFRFPYALLVSALVAFM
                     ALIPILGAFFSASIGALLILVVSPIQALWFAVFFIVLQQLEGNLIYPRVVGSKIGLPA
                     LWVLVAVTVGGNAFGIVGMLVNIPLCSVLYVLLRESVNKRLKKASPFCSDVSPDEQMK
                     H"
     gene            10836..11672
                     /locus_tag="VHR42_02225"
     CDS             10836..11672
                     /locus_tag="VHR42_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3026035.1"
                     /translation="MLEKSDLRVETEILCFRVINQDNENYRKLPGKTLEILLTKPDGS
                     NGEWALPGGAVGSEESLETAAARLLGEETGIDQVYMEQLYTWDKIDRNSGSRVISCSY
                     LALVGSTDGGRDFRGKHCQTGWYAVSCRRKSENRSFTADGSVTRLIYDLELSGNEQRL
                     TSAVEWIRTVQGKKVTVDLTTTGSEGLDPNHSKIIQYGIQRLRSKIGYTDLAFYLVPG
                     MFTLTELQQVYETILDKPLLKANFRRKIASMVTETDQYERRAGHRPSRLYRPNPERQG
                     QN"
     gene            11697..12389
                     /locus_tag="VHR42_02230"
     CDS             11697..12389
                     /locus_tag="VHR42_02230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX3026036.1"
                     /translation="MKNSEQAELWAGELEKSDRSIRRQSAPLDFESLAPEQTALLVVD
                     MINGFAKSGSLSSGRVGALIPSVAELTGRCREKGFKMIFFADSHPEDSPEFQFYPSHC
                     IRGTRESEIVDELKGQGRVIEKNSTNAFLEPEFQTFLKDNSEIRNFLIAGCCTDICVL
                     QLALTLKTHFNRLDERSRIIVPRGAVDTYDAPAHPAELWNLFSFGCMLANGIELAESL
                     TPRDINNGGIRK"
     gene            12386..13828
                     /locus_tag="VHR42_02235"
     CDS             12386..13828
                     /locus_tag="VHR42_02235"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX3026037.1"
                     /translation="MKRENSDNLTMLTDFYEITMANGYLKSGIGEKIAYFDLFFRRIP
                     DGGGFAIMAGLEQATDYLKNLNFTRQDIQYLESKNMFSGEFLNYLKNFKFCCDVWAIP
                     EGTPIFPYEPIVTVRGPAIQAQFVETMLLLTINHQSLIATKANRIVRAAKGRPVMEFG
                     SRRAQGGDGAILGARAAFIGGCSGTACTIADEEYSIPALGTMAHSWVQMFRSEYEAFC
                     AYARIYPADCTLLVDTYNVLKSGVPNAIRAFREVLLPLGYRPRGIRIDSGDITYLSQE
                     ARKMLDDAGFDDCGIVASNSLDEYIIDDILGQGAQINSFGVGERLITSRSEPVFGGVY
                     KLSAVEEEGKIVPKIKISENVEKITNPGFKQVWRLFDQKSGKSMADVITLADEVIDDT
                     LPYEIFDPEYTWKHKVLTGFTAKRLMTRIFNHGDCVYSGPELKEIQGYCKSQIETLWS
                     EVLRFENPHRYFVDLSLPLWNLKQDLLKNR"
     gene            13940..14233
                     /locus_tag="VHR42_02240"
     CDS             13940..14233
                     /locus_tag="VHR42_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX3026038.1"
                     /translation="MAILGNPFVANVNRQLSPDELIQGIRVDIAGELEAIVGYEAHAM
                     ATDNENARKILFHIADEERHHIGELEQLLYQLSPKDTKFIEEGRKEFNQTLMS"
     gene            complement(14587..14940)
                     /locus_tag="VHR42_02245"
     CDS             complement(14587..14940)
                     /locus_tag="VHR42_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEX3026039.1"
                     /translation="MDFDFEFWNALDKIVSTSEIMIDRPKGTRHPKYTDLLYEVDYGY
                     LRNTSSMDGGGIDIWRGTDKDQKIDAIMCIVDLFKRDSEIKILIGCTEEEKKKIYKLH
                     NESEYMKGIMIFRKN"
     gene            complement(15038..>15271)
                     /locus_tag="VHR42_02250"
     CDS             complement(15038..>15271)
                     /locus_tag="VHR42_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HEX3026040.1"
                     /translation="ECKYREQMECKGCVNISKPFWGDSCPVKSCCESKHKEHCGLCEE
                     FPCALLNKFSYDKEQGDNGKRIEQCRKWCDFTK"
BASE COUNT         4019 a         3501 c         3934 g         3817 t
ORIGIN      
        1 aaattgttgc tccgaaagag aagcgaaaaa gtcacttcgt ttgaaaaatt cctgacattg
       61 tttcgctggt cgataaagcc gtcctcatag agggcggctc tacttatgaa ttaaaaaaac
      121 acgaatttta atagaaaagt aataattata ggctataatc aaaattcagg aggtttttgc
      181 tgatgtttat tgacttttca aattttttgc cccgtgtcgg tttgcggaga caatgtaggg
      241 ttctatatag aaaaatgata acgaaaaagc cggtctttta tataacgctg acagtcacgg
      301 tgattataac ggtcatatcc gtaatatttt gcgttgtctt ggcaaaaaga aaacatgcca
      361 tagggcaaat caatgccgga tacaattcct cggcagtcac caaaaaagca aaaacagtaa
      421 ttatagatcc aggtcatggc gggccggatc cgggtgcaac cggtgtaggc ggggttctgg
      481 aaaaaaattt gaacctgatt attgcagaaa aactgaaagc ggattttgaa acagagggat
      541 acactgttat tatgacgcgt aatgacgaca ggtctattaa tgatcccggc tgcatatcgc
      601 tgtatgacaa gaaaaacagc gacctaaaca atcgaatcaa aataggagcg agccacccgc
      661 aagcaatatt cataagcatc catcagaact ttaacgataa ttccaactac accggaacac
      721 aggtctatta ttcagtaaat aaaccgggca gcaaattact tgcacaggat attcagtcgg
      781 aaatcaggac tgatttgcag ccaagtaaca gtcgagaaat acaggagcaa ggcaagcttc
      841 gagtactgtt aaaggcggag ataccggcgg ttttgattga atgcgggttt ataagcaacg
      901 ccaatgatac tcataatctg gaaaacagca gttatcagga aaggcttgac gctgcaattg
      961 tcagggcgac agatacatat atttataatg ctgcaaatta gcaaggacct ttcagccatg
     1021 gactttctga tcatacctca ctaaatttac cccgcttaaa accatcattt tttataccga
     1081 catcaccgat tttactttat caattaattt tggttggatg agggggtagg tccagtctgt
     1141 gggtagttaa ccgcgcggat acaagccaaa gccgctatgg tatttttaag tatcacagcg
     1201 gctttctgat cggggaaatt ctgtaactct ctgtttcagt tttcaaggga aacggttaaa
     1261 atcaatgccg gtctcttttt cagaaatcgt ttattgaagc agagattcgt cgaacgagtc
     1321 aaaccagcct tcaacggttt tggcaatcga gtcgacacag gccgggtcga aggggcttct
     1381 cagccccgcg ccggcaacaa ggccgttaaa gacctttgtt cccgccatgc ggcaaagttt
     1441 gagatacttt tcccacgcgg cgcttttctc tttccggctc atttcccaga attccaaagc
     1501 gcaggtctgg gcaaggcaat agtcgatata ataaaacgga tattcaaata catgcagctg
     1561 acggaggaag gttccgccgt tgccgaaaaa tgggtcgccg ccgaaattga ggtggggcag
     1621 ataggcttct tcaagctttt tccacgcctg tctccgttcc gcgggcgtca tctcgggatt
     1681 ctgatagaca atgtgctgaa actcatcaat cgcgcagccg tagggaatga acacaatcgc
     1741 ttcaaaaaga tgcagataat gatattgctc ggctttgttt ccgaagaaca gcggagtcca
     1801 cggccagcag aaaaattcca tcgacatcga atgaatttcc gccgcatcgt tagtcggctc
     1861 acggtaagcg ggataaacgt ctctcgcaag ataaaattca aaagcgtgac cggcttcgtg
     1921 ggtcaagacg ttgatatcgt catgcgtgcc gttaaaattg gcgaagataa aaggagattg
     1981 gtaatcgggg agcgcgatac agtagcctcc ggcggcctta tttgcccgtc caagcacgtc
     2041 gaacaggccg ttgccgatca taaagtcgat gaattcaccg gtttggcgcg acatttcatg
     2101 atacatcttt ttcccgtttt gaaagatttc ctccggtgtt ccgtgcggaa gaggattgcc
     2161 gccgggaaaa agggtttttt catcgtaata catcagttga tccagcccga gcctctgacg
     2221 ctgtttttca tgaagtctgc gggtcagcgg cacaattttt tccttaaccg actgccggaa
     2281 tgccgcgacg tcttcgggct tgtagctgtt tctcatcatt ctcagataac caagctcgat
     2341 gtaattttta tacccaagct tttttgccat tttgtcgcgg agtttgacca gcttgtcgta
     2401 gatgtcgtcg agctcgcgct catttttcag aaaaaattcc gtccgggccg cgtaggcgct
     2461 ttttcgggtt tcgcgttcag aggaattcat ataataacca agttgggata aattcagggt
     2521 ttttcccgcg aatttaatgc ttgcggacgc aatcagattt gtatattggg ttttcagccg
     2581 gttttcctcc gccatatcgc cgatcagacg gtcgtcaaaa gatttcaggg cgatttcggc
     2641 attttggaaa agcagcgggc cgagcttttc agtaagctcc gcgcggtaag gggaatcgac
     2701 caaagccttg taaaagttca ttgagagggc ttcaaatttc ggcccgtttt catcgtaata
     2761 ttctttttcc tttgcgtaaa aagggtcggc ggtgttgacc gagttccgga tatagctgag
     2821 ggaagacatg gtgacgtatt catcctcaag tctgtaaaat tgcccgagac tgtcaacctg
     2881 ctcggcggcc gaagcggaat tacggaactg agacaggatt ttttgggatt tttcctgata
     2941 agaaggaaaa tcgacttgct tatattccat ctgtgaaaat ttcataggat ccctccgtca
     3001 atatcgttct gaataagata cattttatac caaatgcttt cgagaatcaa acattaagag
     3061 aagaaaacag ttaaaatttt aataaatggg caacattacc attgtattat ggcctttctt
     3121 gttaaatttt gcccggtcac acctgcggga tcggcgcaaa ggcttcctgg gccgtcggat
     3181 tttattattt tattattcgg acaaggcata ggaaataatg cacattgcag ggaaatctgc
     3241 tataatatta atcaatatta atcgttatgt gtgagaaaaa agttccgccg cgtcaatgaa
     3301 cggcggggaa gtggaagttg ccatggaatc cgaaaaattt cttcagcgcg ccgaagagct
     3361 tcggcggctg attcaatacc ataaccttcg gtactatgtt tatgacagcc cggaaattga
     3421 cgactatgaa tacgatatgc tgctgcgcga gctcgagcag atcgaagaag aacacccgga
     3481 aattatcaca cccgattcgc ccactcagcg tgtgggcggg caggcggtcg gcctgttcgg
     3541 gaaggttacc catgaagtgc cgatggagag tcttcaggat gttttctcca aagaagaagt
     3601 tctggccttt gatcagaggg tcactccggc gatcgggaaa gcagtctatg tcgtcgagcc
     3661 caaaattgac gggctttccg tctcccttga gtatgaaaac ggcgtgttta ttcgcggctc
     3721 aaccagaggc gacggcatcg ttggcgaaga cgtcacgtcg aatttaagaa ccatccgctc
     3781 cattccgctc aggctcaccc ggccggttcc ttttctggaa gtccgcggcg aagtttatat
     3841 gccgagaagc agctttctga aactgaccga acagcaggag ctgaatgagg aaaagccctt
     3901 taaaaatccg aggaacgctg cggcagggtc gctgcggcag aaaaatccga agattacggc
     3961 ttcacggggg ctcgatattt tcatttttaa tattcagcgg attgaaggcg aaacggtcga
     4021 tacccattcc ggcggccacg agctgctcaa aagcctcgga ttccggattg ttcccaatta
     4081 ccggatctgc gacgccattg aaacggcggc ggaagaaatc gacgagatcg ggcagatgcg
     4141 gggaaaactg ccgtttgaca tcgacggagc tgtgattaag gtcaattccc tgccggcccg
     4201 cacgcgtctg ggaagtacgg cgaaattccc gcgctgggcg gtcgcctata aattcccgcc
     4261 ggaggagaag gaaacgcggc ttcttgatat tgaaattaat gtcggaagaa ccggctccct
     4321 gacgcctacg gcggtgctgg agcctgtgtt gcttgccgga acaacggtcg gccgggccac
     4381 ccttcacaat caggatttta tcaccgaaaa gaaaattaaa atcggcgatg tcgttgtcgt
     4441 ccgcaaggcg ggcgatatta ttcccgaaat cgtcagcgtc gccagggacg agggaacgca
     4501 gccctatttg atgccgaccg tctgcccgtc ctgcggccag ccggtcatgc aggagcagag
     4561 cgaggctgtg ctgcgctgca ccaacgccga atgtccggcg cagctgatcc gccatctgat
     4621 tcattttgtt tcccgcgacg cgatgaatat cgagggcctc ggcccggcgg tgattgaagc
     4681 gctctctgcg aaagggcttg tcaaatcacc ggccgacctg tatgatataa aagcggaaga
     4741 cctcatctcg atggaacgga tgggtgaaaa atccgcttcc aacctgatta acgccatcga
     4801 gcgttccaaa accagcgatc tcagccggct tgtttatgcg ctgggcattc ggaatgtcgg
     4861 tcaaaaggca gcgaagttga ttgccggccg cttccgggat atggacaggc tcttttccgc
     4921 cggtgttgag gagctgaccg ccatcgacga gatcggggaa attatcgccc ggagcgtcgt
     4981 gaactttttt gcccttgact ccacggccca tctggtcgaa cggctcaggc gggcgggggt
     5041 taatatgacc gaacagagcg cctccggcgg cgaagggcgg ctgaaaggca tgacttttgt
     5101 cctgacgggg actctgcctg gctttacacg gagcaaggct gccgaaatca gtgaaaagct
     5161 gggcggaaaa gtcacgggaa gcgtttcgaa aaaaacttct tacgttctgg ccggcgagga
     5221 cgccggaagc aagcttgtaa aagcgcagca gcttggcgta acggtcatcg acgaaaagag
     5281 gttccgtgaa ttaattcaat aatatgccgc ttccgacggc atgcattctc ccgaattcgg
     5341 ccgcggggag aagctgatag aaaattccgc agcggcagcg gggaaagaag catcatgaca
     5401 gagatgaata ttgatatccg ccatatcgca aaattggcaa gacttcatgt ggatgaagct
     5461 gagatacaga aactgggtaa agaaatggcc gccattatcg gaatggttga aaatctgccc
     5521 gaatttgaaa acgcgcgtct ggagctgagc gagaaggacg ccatgaagct tcgggaggac
     5581 gaagtcagac cgtccatgtc ccggaaagaa gtgctgaaaa acgcgcccga aacagaggcg
     5641 ggctgtattg tagtgcccaa aattgtggag taacagttca gattcaggag ggaaatattc
     5701 atggagcttt acgaatatac agccgcaggc ctttccaaaa tgctgaaaga gaaaaaatgc
     5761 ggcgcgaggg aaattgccga gtcggtattt tcccggatcg acagcgtcga ggaaaaagtc
     5821 ggcgcatatg tcacgctgac cagcgaactg gcgctcagac aggcggatga aaccgaccgg
     5881 aagattgctg ccggagccga aatcggcccg ctcgcgggca tcccggttgg aatcaaggac
     5941 aatctctgca ccaaagacgt cctgaccact tgctcatcga aaatacttgg aaatttcgtt
     6001 ccgccttacg acgccaccgt agtccgtaaa ttgctggacg cgggcgttgt cgtgaccgga
     6061 aaaatgaata tggatgaatt cgcaatgggg tcttcctgcg aaacgtccgc catgaaaaaa
     6121 acacataatc cccatgacct gacccgcgtc cccggaggat catcgggcgg ctccgccgcg
     6181 gcagttgcgg cgggagaagc ggttctcacc ctcggctctg ataccggcgg atcaatccgt
     6241 cagccggcgt cgttctgcgg cgtggtcggg ctcaaaccga cttacagcgc tgtttcccgg
     6301 ttcgggcttg tcgctttcgc gtcctccctc gatcagatcg ggccgtttgc ccgcagtgtg
     6361 gaagacgccg cgatgctgtt tgacacgatc cgcggccacg acgattatga cgcgacgagc
     6421 agccctcttg accatccgtc gatcgcggat aagctcgatg gaaatgtgaa gggtctgaaa
     6481 atcggcatcc cggatgaatt tttcggggaa ggcgtggagg atggtattcg acataccgtt
     6541 cttaaagcgg cgaagaaact tgaaagtttg ggagctatcc tgatgaagat cagcttgccc
     6601 agcacaaaat tcgcgctgcc cgcctattac atcatcgcct gcgcggaggc ttcctccaat
     6661 ctggcgcgct tcgacggggt caaatacggc taccgggcgg agaaatatga aaatctcatt
     6721 gacatgtatg agcgttcccg cagcgagggt ttcggccccg aagtcaaacg gcgcatcatg
     6781 ctcggaacat ttgtcttaag ctccggctac tatgacgctt attataaaaa ggccaagctg
     6841 acccagaacc ggatcagaag cgaatacgac gatgcgttcc aaatctgtga tgtcatcctg
     6901 acaccgacgt cgccgggaac agcgttccgg ctcggcgagc gcgcggatga tccgctgaaa
     6961 atgtacgcgg ccgatatctg cactgtgccg gtcaatattg ccgggctgcc ggctctttcg
     7021 cttccatgcg gaatggatgc tgaagggctt cccatagggc tgcagctgat cggcagaaaa
     7081 tttggcgagc agaccatttt aaatgccgga cacgcttatg aaaaatgcgg cggcggcatc
     7141 gccattccca ggatatagga ggatacgcca tgaattacga aactgtggtc gggctcgagg
     7201 ttcatgccga gctgtcgacc aatacaaaaa tttactgctc ctgcaaaaac gctttcggcg
     7261 gagaagtcaa cgcccagtgc tgcccgatct gtacgggcat gccgggaact ctgccgaccc
     7321 tcaacaagaa agttgtggaa tacgccgtgc gaatgggtta cgccctcaat tgcggactta
     7381 accgcatctg caagcaggac cggaaaaatt atttctatcc cgatcttccc aaggcgtatc
     7441 agatttccca gttcgatgtt cctttgtgtg aaaacggtta tcttgacgtc tgggtcgacg
     7501 ggcaggaaaa acggatcggc gtcaccagaa ttcatattga agaggatgcg ggaaaactgc
     7561 tccacgatga gagtttttcg ggctctctgg tcgattttaa ccgctgcggc gttcccttga
     7621 ttgagattgt ttccgagccg gatatgcgaa gttccaaaga ggcgaaggcc tatctggaga
     7681 cgatccgcag tattctgctt gcgcttgata tttccgactg caagatgcag gaaggctcaa
     7741 tccgctgtga cgtcaacgta tccgtccgtc ctcagggcag tgatacattc ggaacccgct
     7801 gtgaaatgaa aaatgtcaat tccttcagcg gggcggtcag agccattgaa tacgaaacca
     7861 aacgccagat cgaaattatc gaagatggcg gggttatcgt gcaggaaacg cgccgatggg
     7921 acgatgtaaa gggaatgagc tttacaatgc gtagtaaaga ggatgcgcag gattatcgct
     7981 attttcccga gcctgacctt aagaccatcg ttctggaaga agatgatctc cgcaggatga
     8041 aggaatccat accggagctt cccaataaga agctgatccg ctatatgaaa gactataaac
     8101 tgccggagtt tgacgccaat ctcctgtcgg aaaatcccga aaaatcgaaa ttctttgaga
     8161 gctgtcttga agcggcgaaa gtcgagccga aaacactctc caactggatt gtgggcgatg
     8221 tgaccaggat tttgaatgaa caaggaatcg gaattacgga atccgttctt acaccggaaa
     8281 aactgacggc gttgattcag ctgatcgaca aagggacgat ttccaacacg gcgggcaaaa
     8341 cggtgctgga tgagatgttt gcgacgggcg gacggccgga tgatattgtc cgggaaaaag
     8401 gtctttccca aatcagcgat gcatctgctc tggaagagct ggccaaaggc gtgcttgccg
     8461 acaatcagaa atcggtcgat gattaccgcg gcggcaagac caatgcgctg gggtatcttg
     8521 tcggccagtg tatgagggct tcaaaaggca aaggcaatcc ccagaccatt cgcgagatcc
     8581 tgctgaaagt actgcaaagc gaaggcagct aaaaacagcc gatcaggcag acgggttgct
     8641 tttgaaagaa gaattcggcc ggagatttac gataaaaccc ggccggaaat atggaggagt
     8701 catgtatgga ggattcggcg ctgtttacgg tctcaaccgt aatggaaaaa gaggattacc
     8761 gcgcttttat gaatttggcc acttttcgca aatcgccggt catcatccct cttctgctat
     8821 tgataacggc ggccatcgct ctgattataa cagtggtttg cggagcgttc agcttgctgg
     8881 tgtttatgat ttgctggctt gtggaactct tgactatact gatatctttt tacattaaaa
     8941 ttgagaataa gatcaaaaaa attgagaaga caaatagcgg ggcgactttc ggctcgcggc
     9001 agacaatcaa tttttttgat gaatacctgg tggcttccaa cgagggaatc gaaggttcga
     9061 acaagataaa atatgagcag ttttatcagc ttatggaaag taaaaattat tttttgcttt
     9121 atttcaacgc cacaatgggc gcgctgatcc gcaaaaagga tattggtccg ggcgaacttg
     9181 acagcttaag ggagcatttc cgaaataagt tcggagcgaa attcagaaag atatagcttt
     9241 ccggagaggt tcagagttcc aaagactttc tatgatctta aaaaccaaag agcagtgaaa
     9301 atatggcggt taacaggaga ataaagaatt gaaaatggaa ttcgaaaaaa gcggcatgaa
     9361 aaaatggatt ttgctgatcg ccttcggtat tttattttat ttgggaatgc aaaacctata
     9421 tcgtattcct gacctttcga attatattgg cggcattttt gaaccgattc tgctcggttt
     9481 ggctctcgcg ttcgttttaa acgttttgat gtttcaagtt gagagtcgcc tttttgctcc
     9541 tttaaaccgt cgttatacaa aaaagtggcc caaaatacgg cgcccgctga gtattcttat
     9601 aacgtttgtg atcgttcttg gcgctatcac cttgattttg ctgattatcg taccggaact
     9661 tgttcgcacg attaccagtc tgaccaataa gattccggtg ttttttaatg atctccagac
     9721 cggtctcact aaaatcagca gggagtatcc cggtatcggt gaatatatca gcaacatcaa
     9781 gatcgactgg acgagtatca gtcagatgct ggcgcaatat ggacagcaat ttgcgaaatt
     9841 gctggtcggt tcgactgtcg cggtaacgac gaacgtattc catgcggcga ttacatccat
     9901 attgggtctc gtaattgcga ttaacattct ggcgcagaaa gaaaagctgc aaaaccagct
     9961 gaaaagggtt ttgtatgcgt atttaccgca aaaggcgact tcccggttca ttcggctttg
    10021 cagcctcaca aaccatgtct tttcgaattt tattgccggc cagtgtacgg aagcttgcat
    10081 ccttggtacg ctctgtttca tcggtatgca gatttttcgc tttccgtatg cgctgctcgt
    10141 atcggcactt gtcgcattta tggcgctgat tcccattttg ggggcatttt tcagcgcttc
    10201 gatcggcgca ttgctcattt tagtcgtcag cccgattcag gcgctttggt ttgctgtgtt
    10261 ttttatcgtt cttcagcagc tggaaggcaa tctgatctat ccgagagttg tcggctcgaa
    10321 aatagggctt cccgctcttt gggtattggt agccgttacg gtcggcggca atgcgttcgg
    10381 cattgtcgga atgctcgtta atattccgct ttgctccgtt ctgtatgtgc ttcttcgcga
    10441 aagcgtaaac aagcgtctaa aaaaagcatc gcctttttgc agcgatgttt cccctgatga
    10501 acaaatgaag cattagccca cgttttcagt atccgaacgg aatggctgca tttgcggcaa
    10561 agccgctgcc ccgatctttc ttttaccgct gtcccaataa gagtagaaag cttgagcctg
    10621 ccacaaaagg gcaggctcgt tttttatgta tcatccaaat ggaaactgcg ggattggcga
    10681 gagctccact gcgatcctta aattaaatga gccggcgaag tttcttttca caatcgtatt
    10741 tgacaagcag gaattaactg cctataatga tagtaataaa tagataatat cagaatggtt
    10801 ctaacaaaaa agataccggg aaggaggaag gcacggtgct tgaaaaaagc gatttgcgtg
    10861 ttgaaaccga aatcctgtgt tttcgcgtca tcaatcagga taacgaaaat taccggaaac
    10921 tgcccggcaa gacgctggaa attcttctga caaagcctga cggttccaat ggcgaatggg
    10981 ctcttccggg aggggcggtc gggtctgaag aaagtctgga aacagccgcg gcgcgcctgc
    11041 tcggtgagga gacgggaatt gatcaggttt atatggagca gctctatacc tgggacaaaa
    11101 ttgaccggaa tagcggctcc cgtgtcatca gctgctccta tctggctctg gttggctcta
    11161 cggatggcgg cagagatttc cggggaaagc actgccagac cggctggtat gcagtatcct
    11221 gccgacggaa gtctgaaaac aggagtttta cggcggacgg ttcggtgaca aggttgattt
    11281 atgatcttga attgtcgggg aatgagcagc gtctgacttc cgccgttgag tggatccgta
    11341 cggttcaggg caaaaaggta accgttgatc tcacaaccac cgggtcggaa ggacttgacc
    11401 ctaatcattc caaaatcatt cagtatggca tccagcggct tcgcagcaaa atcgggtata
    11461 ccgacctcgc attttatctg gtgcccggaa tgtttacgct cacggagctt cagcaggttt
    11521 atgagacgat tctcgataaa ccgctgctta aagcgaattt cagacgaaaa attgcctcaa
    11581 tggtgacgga aaccgatcaa tatgagcgcc gggcagggca caggccttcg aggctctacc
    11641 ggccgaatcc ggaacgtcag gggcaaaatt aaaaaaagga agggacggtg gtttttgtga
    11701 aaaactcaga acaggccgag ctctgggcgg gcgagctgga gaaaagcgac cgttccatcc
    11761 ggcggcagtc ggctcctctt gattttgaaa gccttgcgcc ggagcagaca gcccttctgg
    11821 ttgttgacat gattaacggt tttgcaaaat ccggttcact cagcagcggt agggtcggcg
    11881 ctttgattcc atcggttgcg gagctgacgg gaagatgcag ggaaaagggc tttaaaatga
    11941 tattctttgc cgacagccat ccggaggatt ctccggaatt ccagttctat ccgtctcact
    12001 gcatccgcgg cacgcgggag agcgaaattg tcgacgagct gaaagggcag ggaagggtta
    12061 tcgaaaagaa ctccaccaat gcttttttag aaccggagtt tcagacattt ttgaaagaca
    12121 acagcgaaat cagaaatttt cttattgcgg gctgctgtac ggacatctgt gtgctccagc
    12181 tggctttaac gctgaaaacc cattttaaca ggctcgacga acgatccaga attatcgtgc
    12241 cgcgcggcgc ggtcgacacc tacgacgctc cggcgcatcc ggccgaattg tggaatcttt
    12301 tttcatttgg ctgcatgctg gcaaacggga ttgagctggc ggaatcatta actccgcgcg
    12361 acataaataa tggaggcatc cggaaatgaa acgggaaaat agcgataatc tgaccatgct
    12421 gacggatttt tatgaaatta ccatggccaa cgggtatctg aaaagcggaa tcggagaaaa
    12481 gattgcttat tttgatcttt tttttcgcag aatccccgat gggggcggct ttgcgatcat
    12541 ggccggtctg gagcaggcaa ctgactacct gaaaaatctg aatttcacgc ggcaggacat
    12601 tcaatatctg gaaagcaaaa acatgttcag cggagaattt ttaaattatc tgaaaaattt
    12661 taaattttgc tgcgatgtct gggcgattcc cgaggggacg ccgatttttc cttacgagcc
    12721 gattgtaacc gtccgcgggc ctgcaataca ggcgcagttt gtcgaaacaa tgcttctgct
    12781 taccattaat caccaaagtc tgattgccac caaggcaaac aggattgtcc gggcggcaaa
    12841 aggcaggccg gtgatggaat tcggctcccg ccgcgctcag ggaggagacg gtgctatact
    12901 gggggcgagg gcagccttta tcggtggatg ttcgggaacc gcctgcacca tagcggacga
    12961 ggaatattcc attcccgccc tcggcacgat ggcgcacagc tgggttcaga tgtttcggtc
    13021 ggagtatgaa gcgttttgcg cttacgcccg catttatccg gccgactgca cgctgctggt
    13081 cgatacttat aatgtactga aatccggcgt tccgaacgct atccgcgcat ttcgcgaggt
    13141 tctcctgccg ttgggatacc ggccccgggg gattcggata gacagcggcg acatcaccta
    13201 tctttcccaa gaagcgcgga aaatgcttga cgacgccgga ttcgatgact gcgggatcgt
    13261 cgcgtccaac tcactggatg aatacatcat tgacgacatt ctcggccagg gggcgcagat
    13321 taattccttc ggggttggcg agaggttgat cacttcacgt tccgaacccg ttttcggagg
    13381 tgtttataag ctttccgctg tggaagagga aggcaaaatc gttcctaaaa tcaagatcag
    13441 cgagaatgtg gagaagatta caaacccagg cttcaaacag gtatggagac tctttgacca
    13501 aaaaagcggc aaatcgatgg cggatgtgat cacgctggcc gatgaagtca ttgacgacac
    13561 gttgccttat gaaatctttg acccggaata cacctggaag cacaaggttt taaccggttt
    13621 taccgccaaa cggctgatga cgagaatttt taaccatggg gattgcgttt attccgggcc
    13681 ggagcttaag gaaatacaag gctattgtaa gtctcagatt gagacgcttt ggagtgaagt
    13741 gctccgtttt gagaatcctc accggtattt tgtcgatctt tccctgccgc tatggaatct
    13801 gaaacaggac cttcttaaaa accgctgaca ggtatatttc tttacacccg tggcaatgat
    13861 gaatagagaa tgaaattcgc tcagcggtgt cggacggcgc tggccggata ccgcggattg
    13921 atcgaaaggg tgtgtaaaga tggcgatttt agggaacccg tttgttgcga atgtcaatcg
    13981 gcagttgtca ccggacgagc tgatacaggg gattcgggtt gatattgccg gtgaacttga
    14041 ggcgattgtc ggttatgagg ctcacgccat ggcgaccgac aacgaaaacg cgaggaagat
    14101 tctattccat attgccgacg aagaaaggca ccatattggc gaactcgaac agctgctgta
    14161 tcagctcagc cccaaagaca cgaagtttat tgaagaggga cgaaaagagt ttaaccagac
    14221 gctaatgtca taaaagaagc agccccggcg ggtattgaag gatttcaccc ctaaagttag
    14281 acagtgtata accacactgt ctaactttag gggtgaaatt ttatttccag aggaatacca
    14341 aagaagcagt atacaggaga atataaacaa gggttttacc atcataatat tgggcacacg
    14401 tgtatgatgt atcataatct gtaaccccat cggaagtttg aggattttct ccaaccgtct
    14461 cctgatctaa aaatttattt tttaaatttg gtgttattaa cttgtattgt tttgtccccg
    14521 gtttttgttg catcaaacct ccacacaata taaatcgtat gaacctcagt tcaatttcgt
    14581 atgatgttaa ttttttcgga atatcataat tcccttcata tactctgatt cattgtgcaa
    14641 cttatagatt tttttctttt cctcttcggt gcagccgatt agtattttta tttcagagtc
    14701 acgtttaaac aagtccacga tacacatgat tgcatcaatt ttctgatctt tgtcggttcc
    14761 tctccatata tcgataccgc cgccgtccat tgaggaagta tttcttagat aaccataatc
    14821 tacttcatat agcaaatccg tatatttggg atgtctggtt ccttttggcc gatcaatcat
    14881 aatctccgaa gttgacacaa ttttatcgag tgcattccaa aattcaaaat caaaatccat
    14941 agtctttact cctatgttga taatcagaaa gaaatctcag tgtaaggcat aaacaaaact
    15001 gcgaaattcg ggaaatgctc ggtactgccc attgtgctca tttggtgaaa tcacaccatt
    15061 ttctgcattg ctcaattctt ttgccgttgt ctccttgctc tttgtcgtaa gaaaatttat
    15121 ttaataatgc acacggaaac tcttcacata aaccacaatg ttctttatgc ttgctttcac
    15181 agcaagactt aacagggcaa ctatctcccc aaaatggttt tgaaatattg acacaacctt
    15241 tgcattccat ctgctctctg tacttacact c
//
LOCUS       DATALY010000073         4504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_189771,
            whole genome shotgun sequence.
ACCESSION   DATALY010000073 DATALY010000000
VERSION     DATALY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189771"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VHR42_02255"
     CDS             <1..706
                     /locus_tag="VHR42_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026041.1"
                     /translation="NNGYAAGNNAGLDYLKEKFGLFDYIIISNPDIEVHLQAIEACRD
                     FLENHDTMALAAPRMHGPDGLPHHLSGWKERTFLCDLAYSSGLLSRTVGMYRETYPES
                     YFQGEYSRVDCAAGSFFMIKGPVFYEIGKFDPNTFLYYEEDILGVRIKRAGYEEAVLN
                     RFGFTHFEGTSVNRSLSMVKKYLTMQKSRIYFQRHYRRINFAQLLVLYAATLLGLTEK
                     LVKAVFYRFHDGHPAA"
     gene            739..1581
                     /gene="rfbD"
                     /locus_tag="VHR42_02260"
     CDS             739..1581
                     /gene="rfbD"
                     /locus_tag="VHR42_02260"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX3026042.1"
                     /translation="MRIFITAGNGQLGTDATAYFSKNHDVISYKDVDLDVTDREKMFR
                     AVGEAKPDVLINCAAITNVDGCESNEELAYRVNADGALITAEAAKAAGAKIVHISTDY
                     VFDGTRKGAYRETDPTCPNNVYGRSKLAGELNVAKACPEHYILRTAWLYGPNGNNFLK
                     TMMKIGAEKGAVSVVTDQVGNPTSTFELIRIIDAVIKTDRYGVYHATCEGCCSWNEFA
                     REIFSMAGMNVKVSDITSEQLVRPAPRPANSNLCKDKLFQNCGYRPTQWKASLKEYFE
                     YTAK"
     gene            1623..2174
                     /gene="rfbC"
                     /locus_tag="VHR42_02265"
     CDS             1623..2174
                     /gene="rfbC"
                     /locus_tag="VHR42_02265"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX3026043.1"
                     /translation="MKLIQTKLEGVMIVEPQVFGDHRGWFTETWSKAKLSQLGLDVEF
                     VQDNHSFSAQKGTLRGLHFQKNPKSQTKLLRCTAGTILDVAVDVRKGSPNYKKWVGVE
                     LSAENHRQLFIPKGFLHGFLTLTDNVEVQYKVDEYYAPECDGSVRFDDPAIGVEWGIG
                     QPVLSQKDLDAPLLKDSAVNFVY"
     gene            2216..3235
                     /gene="rfbB"
                     /locus_tag="VHR42_02270"
     CDS             2216..3235
                     /gene="rfbB"
                     /locus_tag="VHR42_02270"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX3026044.1"
                     /translation="MKILVTGGAGFIGANFVYYLLENHPQDEIVCLDLLTYAGNLKTL
                     DKAMKNPKFKFVKGDISDRDCVFSLFESEKPEVVVNFAAESHVDRSIENPGLFLTTNI
                     IGTQILMDASRKYGVKRYHQVSTDEVYGDLPLDRPDLMFTEETPIHTSSPYSASKAGA
                     DLLVLAYYRTFGLPVTISRCSNNYGPYHFPEKLIPLIISRALNDESLPVYGTGANIRD
                     WLFVTDHCAAIDLIIRNGRVGEVYNIGGHNECTNLHVVKSILKELGKPESLIHYVTDR
                     PGHDRRYAINPEKIEKELGWRPTYKFDEGIKVTVRWYLDNQQWWKDIINGDYQNYYDK
                     MYSNR"
     gene            3282..4208
                     /locus_tag="VHR42_02275"
     CDS             3282..4208
                     /locus_tag="VHR42_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026045.1"
                     /translation="MNSSVNEPHDSSGIHSFQDAMNLAGCSFTDTMIGGVFVKIFNSF
                     KVFKRYSYLLQQLVARDFKVKYKRSVLGVLWSVLNPLFMMIILSLVFANLFRMKDATI
                     RDYPVYLLVGLVFFNYFSEATNLGMGAVVGNFNLITKVYMPKYIFPFSKVLSSGLNFL
                     FSLIALYLIIFVEGYKNGITVTWVHIFLPLDMIYIMIFALGVSMFLAALTVFFRDMFY
                     IYGVIITAWTYLTPVMYPISIVKSSKYAWWLLPLMKLNPLYHYIQYARTIVFGGMPSL
                     QQNLICIFASLVTLLIGVLFFRSKQDKFIYYI"
     gene            4249..>4504
                     /locus_tag="VHR42_02280"
     CDS             4249..>4504
                     /locus_tag="VHR42_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3026046.1"
                     /translation="MITVDHVSMKFNLGNDKVNTLKEYLIKMNKRQIKKEEFWALKDV
                     SFEIKPGEVLGIVGTNGAGKSTLLKVIAGVLKPTKGRVELK"
BASE COUNT         1171 a         1005 c         1131 g         1197 t
ORIGIN      
        1 aaacaacggt tacgctgccg gaaataacgc ggggcttgac tatctcaaag agaagttcgg
       61 gctgtttgat tatattatta tatcgaatcc cgatattgag gttcatcttc aggcaatcga
      121 agcctgccgg gattttttgg agaatcatga tacgatggcg ctcgcagctc cccggatgca
      181 tgggcccgac ggtttgcccc accatctttc cggatggaag gaaagaactt ttttatgcga
      241 tctcgcttac agttccggtc tgctttcacg gacggtggga atgtatcggg agacctatcc
      301 ggaatcttat tttcaggggg aatactcacg ggttgactgt gctgcaggtt ctttttttat
      361 gataaagggc ccggtttttt acgaaatcgg gaaattcgat cccaatacct ttctatatta
      421 tgaggaggat attttgggcg tccggataaa acgggcgggt tatgaagaag cggttctgaa
      481 tcgtttcggt tttacccact ttgaaggtac ttcggtgaac cggtcgctga gcatggtgaa
      541 aaaatatctg acgatgcaaa aaagcagaat ctattttcaa aggcactata gaaggatcaa
      601 tttcgcgcag cttctggttt tgtatgcggc aacattgctc gggctgacgg agaaactggt
      661 caaagccgtc ttttacagat ttcatgacgg acatccggca gcgtaaaaac gccggacgga
      721 taaaacggag gacagaaaat gcgtattttc attacggccg gaaacggcca gcttggaacc
      781 gatgctacgg cctatttttc gaaaaaccac gatgtaattt cctataagga cgtcgatctt
      841 gatgtgaccg accgtgagaa aatgttccgg gcggtcggcg aggcgaaacc cgatgttctg
      901 attaactgcg ccgccattac caatgtcgac gggtgtgaat ccaacgagga gcttgcctat
      961 cgcgtcaatg cggacggcgc tttgattacc gccgaggcgg caaaagcggc cggggcaaaa
     1021 attgtccata tctcaaccga ttacgttttt gacggaaccc gtaagggcgc ctaccgcgag
     1081 acggacccga cctgccccaa caatgtttac ggaagatcaa agctggcggg tgagcttaac
     1141 gttgcaaaag cctgcccgga gcattatatt ctgcgcaccg cctggctcta tggcccgaac
     1201 ggcaacaatt ttctcaaaac catgatgaaa atcggcgcgg aaaaaggcgc ggtcagcgtt
     1261 gtgaccgacc aggtcggcaa ccccacttca acctttgaac tgatccggat cattgacgct
     1321 gtgatcaaaa ccgaccgtta tggcgtttat catgccacct gtgaaggatg ctgctcctgg
     1381 aacgagtttg ccagggagat tttcagcatg gcgggtatga acgtaaaggt cagcgacatc
     1441 acatccgagc agttggtaag acctgctccc cggccggcca attcaaatct ctgtaaagac
     1501 aagctgttcc aaaactgcgg ctacaggccg acccaatgga aggcttcttt aaaagaatat
     1561 tttgaatata cagcaaaata aaaattgttt attcgggcaa taatcttgga ggtatcggta
     1621 aaatgaaatt aattcagacg aagcttgaag gcgtaatgat tgtagagccg caggtgttcg
     1681 gcgatcaccg cggctggttt actgagacat ggtcgaaagc caaactgtcc cagcttggcc
     1741 ttgatgtcga atttgttcag gataatcatt ctttcagcgc ccagaaaggg acgcttcggg
     1801 ggctgcattt tcagaagaac ccgaaatccc agaccaaact gcttcgctgc acggccggaa
     1861 cgattctgga tgtggcggtg gacgtcagga aaggatctcc gaattataaa aagtgggtgg
     1921 gcgtcgagct ttcagccgag aaccaccggc agctgttcat acccaaaggg ttcctgcacg
     1981 gctttttgac tttaaccgat aatgttgaag ttcaatacaa agtcgatgag tattatgctc
     2041 cggaatgcga cggaagcgtt cggttcgacg atccggcaat cggggttgaa tggggaatcg
     2101 ggcagcctgt cctttcccaa aaagatttag atgcgccgct attaaaagac agcgccgtca
     2161 actttgtcta ttaatcccgt cttaccttaa acagacgata agtgaggaat taattatgaa
     2221 aatattagta accggcggag ccggatttat cggagccaat tttgtttatt acttgctgga
     2281 gaatcacccg caggatgaaa ttgtctgcct ggatcttctg acctatgccg gcaatctgaa
     2341 aacgcttgac aaggccatga aaaacccgaa gtttaaattt gtgaaaggcg atatttccga
     2401 cagggactgc gtctttagcc tttttgaatc cgaaaaaccg gaagtggtcg tcaactttgc
     2461 cgccgagtcg catgttgacc gctccattga aaatccgggg ctgttcctga ccaccaatat
     2521 tatcggcaca cagattttga tggatgccag cagaaaatac ggcgtcaagc gctatcatca
     2581 ggtttcaacc gacgaggttt acggggatct gccgcttgac agacctgacc tgatgtttac
     2641 cgaggaaacg ccgattcata cgtcttctcc ttattccgcg tccaaagcgg gcgccgacct
     2701 gcttgttctc gcctattaca ggactttcgg cttgccggtg accatctccc ggtgttccaa
     2761 caactacggg ccgtatcatt tcccggaaaa gctgattcct ctgattatct ccagagcgct
     2821 gaatgacgaa agccttcccg tttacggcac aggagccaat attcgcgact ggctgtttgt
     2881 tacagaccat tgcgcggcaa ttgacctgat catccgcaac ggacgtgtgg gggaagtcta
     2941 taatatcggc gggcacaatg aatgcacgaa ccttcatgtc gtaaaatcga tcctcaagga
     3001 gcttggcaaa cctgagagcc tgattcatta tgtgaccgac cgcccgggac acgacagacg
     3061 ttatgccatc aatcctgaaa agattgaaaa ggaactgggc tggcggccga cctacaagtt
     3121 tgacgaaggg atcaaagtga ctgttcgctg gtatcttgac aatcagcagt ggtggaaaga
     3181 tattattaac ggcgattatc aaaattacta tgataaaatg tacagcaatc ggtaatgacc
     3241 gcttctttac gcccgggccc tgatcccagg cggattacga tttgaattcc agtgtaaatg
     3301 aacctcatga cagttccgga atccattcgt ttcaggacgc aatgaatttg gccgggtgct
     3361 catttaccga tacaatgatt ggcggggtat tcgtgaaaat tttcaatagt tttaaggttt
     3421 ttaaacgtta ttcttatctt ttgcagcagc tcgttgccag agattttaaa gtaaaataca
     3481 agcgatccgt gctgggcgta ttgtggagcg ttttgaaccc gctgtttatg atgatcatcc
     3541 taagcctggt gtttgccaat ctgtttcgaa tgaaagacgc gacgataagg gattatccgg
     3601 tttatctgct ggtcggcctt gtcttcttca attatttcag cgaggccacc aatctcggga
     3661 tgggcgcggt ggtgggtaat tttaacctga tcaccaaggt ctacatgccc aaatacattt
     3721 ttccattttc taaggtttta tcatccgggc ttaatttcct gttttccctg attgctcttt
     3781 atctgattat ttttgttgaa ggctacaaaa atggtatcac cgttacatgg gttcacattt
     3841 ttctgccgct cgatatgatt tatattatga ttttcgcgct gggggtaagc atgtttttag
     3901 cggccctgac ggtctttttc cgtgatatgt tttatattta cggggtcatc ataacggcct
     3961 ggacttatct gacgccggtc atgtatccga ttagtattgt caagagctca aaatatgcgt
     4021 ggtggctcct gccgttgatg aaactaaatc cgctttacca ctacatccaa tacgccagaa
     4081 cgattgtgtt cggggggatg ccttcccttc agcagaatct gatctgcatc tttgcctcac
     4141 ttgtgacact gctgatcggg gtgttgttct tccggtcgaa gcaggataaa tttatttatt
     4201 atatttaacg ggattgctgt ttaaccggaa gtttgggggt cgtttagcat gataaccgta
     4261 gaccatgttt cgatgaaatt taacctgggt aatgacaaag tcaatactct aaaagaatat
     4321 ttgattaaaa tgaacaagcg ccaaattaaa aaagaagagt tttgggcgct gaaagatgtg
     4381 tcgtttgaaa ttaaacccgg agaagtgctg ggaattgtgg ggaccaatgg agccgggaaa
     4441 agcacgctgc tgaaagttat tgccggtgtt ctcaagccga caaagggacg tgtcgaatta
     4501 aaag
//
LOCUS       DATALY010000074         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_707234,
            whole genome shotgun sequence.
ACCESSION   DATALY010000074 DATALY010000000
VERSION     DATALY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707234"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VHR42_02285"
     CDS             complement(<1..1189)
                     /locus_tag="VHR42_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026047.1"
                     /translation="MTFHEISKKMLLANFRRYTLFFYCNVFSVALFYCFSALFTNSTF
                     MNPKIVDSMISSNIIAPSVFVGIFLLLSVPYSYQAFLKNRKREYGIFMALGMSHAEVL
                     KNMLFESGVVAGLALGAGLILGTTVSVLFSCILCHVLGISALRWYLNADSYRVTTLFF
                     GLTVLLLWAVTILGFFQTQLIDWMKGKFRAEKTGRASPVLLAAGAILIVVSILTVEDS
                     GKGDVGSILLASLAGVFVGLWLIFANVRSMEPFLAARFPSFGKHHLVEISFVRQHLRS
                     QRRIGAAAVGLIGFSVFFAGLCAAAYPSFMKNVKTYSPYDLVYCQTEGKNQADDASIK
                     SLLSRNGVAVRSEKQVPYLRDTAFNLIAVSEANREFSCSYQVPAGKFITVFPYDLNDG
                     YRRE"
     gene            complement(1176..1934)
                     /locus_tag="VHR42_02290"
     CDS             complement(1176..1934)
                     /locus_tag="VHR42_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026048.1"
                     /translation="MYSLEIKNVWKSYSLRNREKTTVLKDLSLQIEPGELVAAMGPSG
                     SGKTTLLNLISGVDRPNSGSIWIEGRDLSELDWDELALFRRRRLGMVFQDFNLIESLT
                     VRENILIPMILEKKSAEEQEERAEEILRIFAIEDKKDQNIADLSGGQKQRTAIGRALV
                     NHPAILLADEPTGNLDPKSTKEAIDCFLKINREFGTSILMVTHDASVASCCKRVVFLK
                     DGAFLTEIKKTGSRTEFLHSIKETLAGGESDDIS"
     gene            complement(2022..2384)
                     /locus_tag="VHR42_02295"
     CDS             complement(2022..2384)
                     /locus_tag="VHR42_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026049.1"
                     /translation="MGFSCTGKRNIAKIVFLSFALTLLLSFNFKAWSGCYLGGGFEDL
                     VITQNAEWNHSVIANREADQSILFSELCHQIPIPGWSGMFFSRPDPLLLRFVQKCDGV
                     IYNLEKTTPILLSVRKDE"
     gene            complement(2459..3274)
                     /locus_tag="VHR42_02300"
     CDS             complement(2459..3274)
                     /locus_tag="VHR42_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX3026050.1"
                     /translation="MHLFNASSLFNENSNREIMSVINNPEELEKKLVETKEMLLKLYH
                     FKAFNVPEDFTTNSYIIASNHLTDSDAPLIMSYYYEVMHPVLNTYPALFVFAKENCFN
                     GVSIPKELIPILEFEKVFAVDRNSAGGSMAAIRAAENWFAEGKKPKHFLIFSQGTIYD
                     INKDKAEDIERGAFWLARLLDIPVLPAFIEQAVEGAENRLVFGKPIFVPKGCRNFDDI
                     KQLWVDRVIEAENELMKLTGVPAREATLDAEHQTRKRFKPVKADSAHTSNKVG"
     gene            complement(3389..3667)
                     /locus_tag="VHR42_02305"
                     /pseudo
     CDS             complement(3389..3667)
                     /locus_tag="VHR42_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
BASE COUNT         1127 a         1063 c          841 g         1130 t
ORIGIN      
        1 tttcgcgcct gtaaccatcg tttaaatcat acgggaacac cgtaatgaat ttccctgcgg
       61 gtacctgata gctgcaggaa aactcccggt ttgcttcgga taccgctatc agattaaatg
      121 ctgtgtcccg aagatacggc acctgttttt ccgaccggac ggcgacaccg ttccggctca
      181 gcagactttt tattgacgca tcatccgcct gattttttcc ctcggtttgg caatagacca
      241 gatcataggg agaatacgtc ttcacatttt tcatgaagct tggatatgcc gctgcacaga
      301 ggcctgcgaa aaacacggaa aatccaatta acccgaccgc cgcggctcca atccttcgct
      361 gagagcgcaa atgctgccgg acaaaagaaa tttccacaag gtgatgtttc ccgaaagagg
      421 ggaatctggc cgcaagaaac ggctccatgc tgcgcacgtt ggcaaaaatc aaccagaggc
      481 caacaaacac cccggccaga cttgcaagca gtattgatcc cacatcaccc tttccggaat
      541 cttccaccgt cagaatggag actacaatta gaattgcccc cgctgccagc agtacaggtg
      601 acgccctccc cgtcttctcc gcccggaatt ttcctttcat ccaatcgatc aactgcgtct
      661 ggaaaaatcc aaggatggtc accgcccaca gcagcagaac cgtaaggccg aagaaaagag
      721 ttgtaacccg ataggaatcc gcattcaggt accaccgcaa cgcagatatt ccaagtacgt
      781 gacaaaggat gcaggaaaac agcacagaaa ccgttgttcc gagaatcagc cccgccccaa
      841 gtgccaatcc cgcaacaacg ccgctctcga agagcatgtt tttcagtact tcggcatgac
      901 tcattcccaa agccatgaaa ataccgtatt cccgtttccg gttcttcaga aaagcctgat
      961 aggaataagg gacggacaag agcaggaata ttccaacgaa cacgctcggt gcgattatat
     1021 tgctggaaat catactatcg acgattttag gattcataaa cgtggagttg gtaaagagcg
     1081 cggaaaaaca ataaaacagt gcaacggaaa acacattgca ataaaaaaag agcgtatatc
     1141 tccggaaatt tgcgagaagc attttttttg aaatctcatg aaatgtcatc cgactcaccc
     1201 ccggccagtg tttccttaat ggagtgaagg aattccgttc tgctgcccgt ctttttgatt
     1261 tcggtgagaa aagcaccgtc cttcagaaaa acaacccgtt tgcaacagct tgcaacggat
     1321 gcatcatgcg ttaccataag aatactggtt ccgaattccc gattaatttt caaaaaacag
     1381 tcaatcgctt ctttggtcga tttaggatcc agatttcccg ttggttcatc cgccagcagg
     1441 atggcagggt gattaaccag tgcgcggccg atcgcggtac gctgcttctg cccgccggac
     1501 aggtccgcaa tgttctgatc ctttttatct tcgatcgcga aaatcctcag gatttcttcc
     1561 gcacgttcct cctgttcctc tgcggacttt ttctcaagga tcattggtat aagaatattt
     1621 tccctgacgg taaggctttc aataagatta aaatcctgga aaaccatccc cagtcgcctt
     1681 cggcggaaca gagccagttc atcccaatcc agttcggaca aatcccttcc ctcaatccat
     1741 atgctgccgc tgttcggccg gtcaactccg ctgataagat tcagaagcgt agtctttccg
     1801 ctccccgacg gtcccatggc ggcgaccagt tccccgggtt ctatttgcag gctaaggtct
     1861 ttcaatacgg tggttttttc cctgttccga agggaatagc ttttccagac attttttatc
     1921 tccaaactgt acatcagtca atctccaatc cttttcatta agaaatccgt gcagcaaaaa
     1981 agggccggac ttccggctct ttttaaggtt gaagaatgat tttattcgtc ctttcggacg
     2041 gaaagtaaaa ttggagtagt tttttccaaa ttgtatatga cgccgtcgca tttttggaca
     2101 aatcggagca acaacgggtc cggacggctg aaaaacattc cactccatcc gggaatcgga
     2161 atctgatggc aaagctcgga aaataagatc gattgatcgg cctcgcggtt ggcaatcaca
     2221 ctgtggttcc attccgcatt ctgggtaatg accagatctt cgaatccgcc gccgagatag
     2281 cagccgctcc aggctttaaa attgaaagac agcaggagcg tcaatgcaaa ggaaaggaaa
     2341 acgattttag caatatttct ttttcccgtg catgaaaaac ccatgtgccg cctccctgcg
     2401 ctttcagacc atagaggccc tcagccttat caaaagtttg ctctggtctt cgcccctttt
     2461 atccgacctt gttcgaagtg tgggcactgt ccgccttaac cggtttaaac ctttttcttg
     2521 tctggtgttc cgcatcaaga gtggcttctc ttgcaggaac tcccgtaagc ttcattaact
     2581 cattttcagc ttcaatgacc cgatccaccc acaattgctt gatatcgtca aaattccggc
     2641 aacccttggg cacaaaaatc ggtttgccaa aaacgagacg attctcggcg ccttccacag
     2701 cctgctcaat aaaagcaggc aaaacaggaa tgtccaataa tctggccagc caaaatgctc
     2761 cccgctcaat atcttccgcc ttatctttat taatgtcata aatcgttccc tgcgaaaata
     2821 tcaggaaatg cttcggtttt ttcccttcag cgaaccagtt ttccgcagct cttatcgccg
     2881 ccattgatcc gccggcactg ttgcgatcaa ctgcaaaaac tttttcaaac tccagaatcg
     2941 ggattaattc cttcgggata gagacgccgt taaagcaatt ttctttcgca aaaacgaaca
     3001 aagccggata agtatttaaa accggatgca ttacctcata atagtagctc ataatcagcg
     3061 gcgcatccga gtccgttaga tgattggatg caataatata agaattagtg gtaaaatctt
     3121 ccggcacatt aaaagcttta aaatgatata gctttaaaag catttccttt gtttcgacta
     3181 atttcttttc caattcttcc ggattattta taacggacat gatttccctg ttggagtttt
     3241 cattaaacag cgaacttgca ttaaacagat gcatactctg aacctccatt acatttttgt
     3301 ttttgaaatt ttgccgtgct gtattgtgct tccacaaagt catttgtaaa aattgctaag
     3361 tatttatcgg gattactccc ggttgagact attcctgaac cgagatattg gatgccttga
     3421 gccttctcat ggtttttcca aatttatccg tcttagccgc ttcgatgcat tgctgattga
     3481 accgaaccga aaacctattt ttcacccccg cgcgggccga tgccgccacg caataatttc
     3541 cgtcaactcc taccatttcc aattcatcaa tatcattttc acgaaggaaa acggctaacg
     3601 caggattgga aaagcaattc attctatttt tgataaaaat atggtccgaa acaatcgtaa
     3661 ggccccgaac aagctgtgga gtgtaccttt tactttgata aaaaagcgat tcagaacata
     3721 gacgactaag cgcccttcgt tcgtaaagtc ctgaattctt tcattgattc tgggaattaa
     3781 aaattccgga tggtatggaa accgctttgt gtctcttgtt cccccaacat agtcctcctg
     3841 catatctatc actaaaagag ctttcaacca acattcctct ttccgtttgt taacagtctg
     3901 gttttaggtt tcagcattga tattggggca gcccgttccc cactttccgt taattggacc
     3961 atctgtattt tatcacattg aagctaattt ttctattgct taggtctggt gatccgtttc
     4021 aaaacacaga tttttcccac aatgtttaat ttaaattata cttcacatgc gcaaccaatc
     4081 cgtatgcagt caaatgcgac accacggcgc tcaccggcgt aaagagcggc aagacttata
     4141 cattcgccat gacggtaagc g
//
LOCUS       DATALY010000075         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_362803,
            whole genome shotgun sequence.
ACCESSION   DATALY010000075 DATALY010000000
VERSION     DATALY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362803"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VHR42_02310"
     CDS             <1..994
                     /locus_tag="VHR42_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3026051.1"
                     /translation="MVLAVLLPKVLMFIKPKLTQSNIVLDPPSVIILVIILILAVCSY
                     FYKYNTDEMPRSTKIKAPAKSYALMPLVFVVIAINDVIAPATLCQIKDLSKNSIESYY
                     FLGVIIGISVVLLLQKRFAINICNMLNISFALLTIGFVVEIISFQSGGVGFIAAVCFG
                     ISYVIGFVNIYYFAGFMTKKFQSVPFYRVGIILATLYYLAAFIVMEMFKENNMLSPAF
                     MALVCICIVILFFILTPFFIKILYAGEWIEDTYRPDVTRCSRLEAKLRDYKLTPSEIE
                     VCRLLLEGCTLRQISGIQSKAYATINTYCTAIYRKLKINSRTELLVLLRDYMEK"
     gene            1152..1505
                     /locus_tag="VHR42_02315"
     CDS             1152..1505
                     /locus_tag="VHR42_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3026052.1"
                     /translation="MERLKNLLSESKIKPGADKRFPAETPVSAVHTPYGEKLSISDEE
                     KSLFMDLLTPRERDTCLLLLEGYTLKETAKRLGIGYSTANTYQTALYRKLHINTRAEL
                     IINYRDIGRSQDRET"
     gene            1570..2049
                     /locus_tag="VHR42_02320"
     CDS             1570..2049
                     /locus_tag="VHR42_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX3026053.1"
                     /translation="MTDILGGLGGLMKGLSGFMPQDDPDVKLMNAHTQVNEHQTRLTD
                     LYAQIGRKAVEKDGLESYGELADQLKLTQNNLASAENALKSAQQEKDAKERTEREKAE
                     KETCPECGAHNQEGIKFCQECGAKLGTRAKNSCVKCGAELPFGTRFCGECGARQPEV"
     gene            2053..2400
                     /locus_tag="VHR42_02325"
     CDS             2053..2400
                     /locus_tag="VHR42_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX3026054.1"
                     /translation="MAFYKQPCMHCGTYVDRDARFCPACGSHSPFGYLCPSCLRPVEK
                     GSLLCAGCGRPLYVACPICGNRTFVQEKCEQCGGSLMVRCQNRRCGAMQFFENTKCTV
                     CGTKIKLQKSGKK"
     gene            2411..3166
                     /locus_tag="VHR42_02330"
     CDS             2411..3166
                     /locus_tag="VHR42_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX3026055.1"
                     /translation="MLKSFTRNYEDNSTEAGFQFTFYCDICQDGYKSSFTRSETYSKG
                     RGLRGLVQGASMLGQLIGGRAGDLGYSLERGTNILSERFEGMSPEWQKEHEIAFERAQ
                     NEAMQHFHRCHSCNIWVCDSCYNEDEGLCTQCAPRQEVYVAKARAEAMRRNIDEAAES
                     ATVWQGKLESKTTVCPVCGKPAGTGEFCSNCGASLALNKCPGCGRENARGVKFCNYCG
                     ASMAAAKSPVCPGCGSPIQPGLRFCGECGHKLT"
     gene            3294..4013
                     /locus_tag="VHR42_02335"
     CDS             3294..4013
                     /locus_tag="VHR42_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026056.1"
                     /translation="MKKTMIIICTILMALSLTACGVNGAQQAPQTSQNGQASAKTAGS
                     ENLLSAGYADIMKSGKFLIRYKASTTVENVMVDAEITTATDGEAVATIMKTKETTSHS
                     IIKGNAIYMLDDTNKTYSKMNVSQEEKSAKTGDLTDTGNLAYVGKGTGAVNGKNLPYE
                     EYKAGDETLRFFMNGKNLYAIRSKTKDGELTMVVLELTGKIPAETLSIPADFKEGTVM
                     SIPSGIDMAGQQMDTEEAQNQ"
     gene            4116..4574
                     /locus_tag="VHR42_02340"
     CDS             4116..4574
                     /locus_tag="VHR42_02340"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX3026057.1"
                     /translation="MKNQVYELHKSSIGDMNANIMAMLTYVASVVVSWIPVIRYFAWL
                     VPLVLFFMEKQSKFVKFHAMQSFVLNLACAALTFLISVIIGGIVSASVVNIYAAYSAL
                     GLLGFIGFLTMAVCLVITVFAIIAMVKAYGFKEYHIPLAGGIAEKTGGEF"
BASE COUNT         1262 a         1040 c         1227 g         1134 t
ORIGIN      
        1 catggtattg gcggtattgc tgcccaaggt gttgatgttt ataaagccga agctgactca
       61 gtctaatatt gtacttgatc cgccaagtgt cattattctg gtaatcattc tgattttagc
      121 cgtatgttcg tatttttaca aatataatac ggatgaaatg ccgcgttcaa ccaaaattaa
      181 agcgccggca aagtcatacg cgctgatgcc gctggtgttt gtggtgattg ccattaacga
      241 tgtaatcgct ccggccacat tatgccaaat caaagatctg tcgaaaaaca gcattgaaag
      301 ttattatttt cttggggtca taattgggat ctctgttgtc ctccttttgc agaaacgttt
      361 tgcgatcaac atctgcaata tgctgaatat ctcttttgcg ttgctcacga tcggctttgt
      421 tgttgaaatc atcagttttc agtcgggcgg ggtcgggttt atcgcggctg tctgtttcgg
      481 aatttcctat gtgatcggtt ttgtgaatat ttattatttc gcgggattca tgacaaaaaa
      541 gtttcaaagc gttccgtttt atcgggttgg cattatctta gctaccctgt actatttagc
      601 ggcttttatt gtgatggaaa tgtttaaaga aaataatatg ttatcgccgg cgtttatggc
      661 tcttgtctgt atttgtatcg tcatactctt ttttatcctg acacccttct ttatcaaaat
      721 actctacgcg ggggaatgga ttgaagacac ctaccgcccg gatgtaacaa ggtgttcccg
      781 tttggaggct aaactcaggg attacaaact gacgccttcc gagattgagg tctgcagact
      841 gcttttggaa ggatgtaccc tacgtcagat ttccggtatt caatcaaagg cgtatgccac
      901 aatcaatact tattgcacag cgatataccg aaagctgaaa atcaacagcc gcacggaact
      961 gctggttctc cttagagatt atatggaaaa gtaagaaagc cttatataaa aatcaccccc
     1021 tcattagaac taatgagggg gtgattttta tgccatcatg tgaattactg aatagacagc
     1081 ccgtattttt cagaagaaaa taaaattgtt cccgaacgag gccgagatat ggagatgatg
     1141 aaattggggt aatggaaaga ctgaagaatc ttttgtcaga atcaaaaata aagcccgggg
     1201 cagataagag gttcccggct gaaacgcccg tttcagccgt tcatacccct tatggggaaa
     1261 aactcagtat cagcgatgaa gaaaaatcat tgtttatgga tcttcttacg ccgcgcgaaa
     1321 gggatacttg tctgcttttg ctggagggat ataccctgaa ggaaacggcc aaacggctgg
     1381 gaatcggata ttcaaccgcc aatacttatc agacggcgct ctaccggaaa cttcatataa
     1441 acacccgtgc ggagctgatc atcaattatc gcgatatcgg aagatcacaa gacagagaga
     1501 cctgatgatt taaggcaaaa tatggatcgt ctgatcttca tgcagaaaaa ttaagaggag
     1561 gaacctggaa tgacggatat tttgggagga cttggggggc tgatgaaagg cctgtcgggc
     1621 tttatgcctc aggatgaccc cgacgtgaag ctgatgaacg cccatacaca agtcaatgag
     1681 catcaaacgc ggctgaccga tctgtatgcg cagattggcc gaaaggctgt ggaaaaagac
     1741 gggctcgaat catatggcga attagcagac caactgaagc ttacacaaaa caacctggcg
     1801 tcagctgaaa acgccttaaa atcagcgcag caggaaaaag atgcaaagga gcggacagaa
     1861 agagaaaagg cggaaaaaga aacctgcccc gaatgtggag cacataacca ggagggcata
     1921 aaattctgtc aggagtgcgg tgcaaaactg ggaacccgtg caaaaaattc gtgcgttaaa
     1981 tgcggcgcgg agctgccttt tggaacgcgc ttctgcggcg agtgcggcgc aagacagccg
     2041 gaggtgtagg gaatggcatt ttacaaacaa ccctgcatgc attgcggcac gtacgttgac
     2101 cgggacgccc gtttctgccc ggcatgcggc agccacagtc ctttcgggta tctttgtccg
     2161 agctgtctgc gtccggttga aaaaggcagc ctgctttgcg ccggctgcgg gcgccctctt
     2221 tacgtcgcct gccctatctg cggaaaccgg acttttgttc aggaaaaatg tgaacaatgc
     2281 ggcggcagcc tgatggtccg ctgtcaaaac aggcggtgtg gagcaatgca attttttgaa
     2341 aataccaaat gcacggtatg cgggaccaaa atcaaactgc agaaaagcgg aaagaaatga
     2401 gggggattcg atgctgaaat cttttacacg caattatgaa gacaactcca ccgaggcggg
     2461 gttccagttc accttttact gcgatatatg ccaggacggt tataagagct cttttacgcg
     2521 gtcggaaacc tacagcaaag gcagggggct gcggggactg gttcaggggg cgagcatgct
     2581 gggtcaactg atcggcggca gagccggcga tctcggctat tcgctggagc gcggtaccaa
     2641 tatcctgtcg gaacggtttg aagggatgag ccccgaatgg cagaaggagc atgaaattgc
     2701 ctttgagcgg gcgcagaacg aagctatgca gcattttcac cgctgtcatt cctgcaacat
     2761 ttgggtctgt gattcctgct ataacgagga tgaggggtta tgcacccaat gcgctccccg
     2821 tcaggaggtt tatgttgcaa aggcgcgggc ggaagccatg cgccgcaata ttgacgaggc
     2881 ggcggagtcg gcgaccgtct ggcagggcaa acttgagagc aaaaccaccg tatgcccggt
     2941 gtgcggaaaa cctgccggaa caggggaatt ctgcagtaac tgcggcgctt cgcttgcatt
     3001 gaacaaatgt cccggctgcg gcagggaaaa cgcccggggc gtgaagttct gcaattactg
     3061 cggagcgtcc atggcagcgg caaagtcgcc cgtctgcccc ggatgcggtt cgccgattca
     3121 gccggggctg cgtttttgcg gggaatgcgg gcacaaactg acctgacggt ttcggggata
     3181 cgatgctctg tatattgttt ggaagtttgt atctttaatc tgctaatgag gggttttatg
     3241 gcaaaaaata cgacaagcgc gtttcgacaa aaccgtcctg aggaggaatc aaaatgaaaa
     3301 aaaccatgat tattatttgc acaattttaa tggccctttc cttgaccgcg tgcggcgtca
     3361 acggcgcaca acaagcgccg cagacttcac aaaacgggca ggcatccgca aaaaccgccg
     3421 ggtcggaaaa tctgctttcc gccggctatg cggacattat gaaaagcggg aaattcctga
     3481 tccgctacaa agcgtccact accgtcgaga acgttatggt ggacgccgag atcacaaccg
     3541 ccaccgacgg cgaggccgta gcaactatta tgaagacaaa ggagacgacc agccattcga
     3601 tcattaaggg caacgcgatt tatatgctgg acgacacgaa caagacctat tccaagatga
     3661 acgtcagtca agaagaaaaa tcggcgaaaa caggagatct taccgatacg ggcaatctgg
     3721 cctatgttgg taagggcacc ggtgcggtaa acggaaaaaa cctgccctat gaggaataca
     3781 aagccggaga tgaaacgctg cgatttttta tgaatggaaa gaacctttac gccatcaggt
     3841 ccaaaaccaa ggatggtgaa ctgaccatgg ttgttcttga acttaccggc aagataccgg
     3901 cggaaacgct ttccatccct gccgacttta aggagggcac ggtcatgtcc atccccagcg
     3961 gtattgacat ggccggtcag caaatggata cggaagaagc gcaaaatcag taaaaggcac
     4021 aggaattgat tcccgcaatc cggaaatttc agcggcttgg ccggagcggc agatatcggt
     4081 aatatccata taaattatcc tgaggagaat gagtcatgaa aaatcaagtt tatgagctgc
     4141 acaaatcatc gatcggcgat atgaatgcca acattatggc gatgcttacc tacgtcgcat
     4201 cggtggtggt aagctggatt cccgtgatac gctatttcgc gtggctcgtg ccgctggtac
     4261 ttttctttat ggaaaagcag agtaaattcg taaagttcca cgccatgcag tcttttgtac
     4321 ttaatttggc ctgcgccgcc ctcacctttc tgatatcggt aatcatcggc ggtatcgtca
     4381 gcgcatcggt tgtcaatatc tacgcggctt attccgcact ggggctgctg ggcttcatcg
     4441 gcttcctgac tatggccgtc tgtctggtca ttacggtatt tgcaattatc gcaatggtta
     4501 aggcgtacgg gtttaaagag tatcacattc ccctcgcagg agggatcgcc gaaaaaactg
     4561 gtggggaatt ttgaaaaaaa ttagagctcg gcattgtatt ttacaggtat aacacgaaac
     4621 ggttccgcca ggctgtattt ttccaccggt tttttgatga aat
//
LOCUS       DATALY010000076         8145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_707775,
            whole genome shotgun sequence.
ACCESSION   DATALY010000076 DATALY010000000
VERSION     DATALY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707775"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VHR42_02345"
     CDS             complement(<1..416)
                     /locus_tag="VHR42_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3026058.1"
                     /translation="MIEVKNVQKSFGDFTVLENCSCKIEKGSVYGLVGANGAGKTTLL
                     KLVSGVYRPDGGEVLIDGSLVCSDASVRRRLFFVPDDLYFLPQASMNRMAGFYRGYYP
                     DWSEKLFQKLTQVFELNPKQRLNSFSKGMQRQAAIIL"
     gene            complement(413..790)
                     /locus_tag="VHR42_02350"
     CDS             complement(413..790)
                     /locus_tag="VHR42_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3026059.1"
                     /translation="MISIDFRSRVPIYEQIINQISELVTLEILKPNDQLPSIRSLASE
                     LKLNVNTVKRAFTELESAGMIYTLSGRGSFISEKALQNHVLKSKALVQIETALRVGRS
                     SGLSKNEVSELLDRIYDKKEDSN"
     gene            990..1610
                     /locus_tag="VHR42_02355"
     CDS             990..1610
                     /locus_tag="VHR42_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026060.1"
                     /translation="MLQDTEEIQKLVEQLDGVTAAKIVVGSQGQMTEIHILADRSKSP
                     KQLSRDIQSAITAATGVCVEHKIISIAQVGREALKQAPRLKIENVEISSGVDGFSAKV
                     TLAADHKQYVGSANGLSTAAGRYNTVSKACLNAVQEYSAACAFYVSDVIKIRIANTDE
                     INVAISYFDKDIEKILTGAALVKNDEYYGIVRATLNAVNRVIPVLS"
     gene            2020..3321
                     /locus_tag="VHR42_02360"
     CDS             2020..3321
                     /locus_tag="VHR42_02360"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX3026061.1"
                     /translation="MHSKSEKFFLATVISSGMILLLLTTYFCPILDWKDFLFLSILGI
                     ITESQAVEFDDANGISITSAIIICAILLGNPAVVLWVSAVSVSLAIVKKHGGGFYSIF
                     NIPYYATLFNLSLYVLTASANMAAYYLLGGTIIQKRYAAFSSTVHLISLNSVPLLISI
                     FTGIFVNTVLVALLMAIRSHESILKIWSSKLLWSIVNLVIVGLIGVIITALYAAYDWF
                     IVLLFFAPFTLARYVFAYYKDLQHSYLQTVKSLTAAIETKDEYTIGHSKRVEFYSGII
                     AKEMKLSARRCNSLRYAALLHDIGKIGIDEEILNKKEKLSDEDWKLIRNHPDKGAHIV
                     EDIEFLSSSVDIIRSHHERFDGKGYPRGLSSNELPLEAMILAVADSYDAMTSDRPYRK
                     ALSQDTALVELQHGAGFQFSPEVVRSFDRAIHKKENQIHVA"
     gene            3311..3913
                     /locus_tag="VHR42_02365"
     CDS             3311..3913
                     /locus_tag="VHR42_02365"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEX3026062.1"
                     /translation="MLLEAAIAGIAAGLLLKGSLRCLLDCKLRFFPLLLASVLLSLLP
                     KMPGINILLSRFGAPAAFALAVLRYGALLAFVFFNRGKTGLPIVGAGGFLNALVTIAN
                     GGKMPVSQAAVAAAPAGKDTILLQQGEILNYCIAGARTRLAFLCDCIKLRGIFLYFLS
                     FGDILIAVGIFLFLLAEMKPRILTGKPPFGNSRGNIENGK"
     gene            3971..5752
                     /locus_tag="VHR42_02370"
     CDS             3971..5752
                     /locus_tag="VHR42_02370"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX3026063.1"
                     /translation="MKSIRNYLQKNVLLADGAFGTYISSLLGRNMPSCELLNLKDPEL
                     VLNVHKQYVAAGSRLIGTNTFSANTFSLETNRAETDEIIRSGVSLARKAAGENVYVAA
                     DIGPVPQGDRDSDAIHEEYCRIADAFLAEDVQIFIFETFADSDAPLFLAEYIKNRVPD
                     AFILTNYTVTSDGYSRMNVKGDKLIRSSADSGFADAAGFNCGCGPAHLVKYASGIDFG
                     GLFPALLPNAGYPQMENDFYLYSGSPEYFASKMLEAVQCGFKIFGGCCGTTPEHIHRL
                     SLALKNVSGQRPIKKESGVEISVPTVRRSELFPETGRKIFIAELDPPFQSDLTKLESC
                     AGFLRDNGVDAVTVADSPMARPRVDSIAVAARLKRKFGIEAVPHITCRDRNLNALRSS
                     LLAAHMENLRNILAVTGDPVSETDRGSVKGVFNLNSAGLCGFIRDMNGDAFQNDPMFC
                     GCAFNVNAKNPEQELHRLEKKINSGATFLLSQPVFTGRSVETLRKAKKMGVKVAAGII
                     IPVSYRNALFLANEMPGITLPQQNVLERFSPDMTREQGEEAGLMAALEVARKAADFAD
                     GFYVIAPFNRVYLTVEFMKRMKRELRL"
     gene            complement(5974..6768)
                     /locus_tag="VHR42_02375"
     CDS             complement(5974..6768)
                     /locus_tag="VHR42_02375"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX3026064.1"
                     /translation="MIIIGEKLNSSVPSVKALMDAKNSGEIIKLARAQTENGARFIDI
                     NAGMFLEKEPEMLAWLAGTLAAEGFALSIDTPSHEAARAALEAAGTEGNVINSVTTQK
                     ERFENMAALAAEHRCGVVALCMQDAGTPENLESRLSTADELVSRLTSRGVSAEDIYID
                     PMLRPLGADETAGLDALETISRLSKAYPECSVICGLSNLSFGLPKRKIINRAFAVAAV
                     CNGLNAAILDPLDRDLLGLVSAAEAISGKDEYCLEYISKCREGRIG"
     gene            6919..7698
                     /locus_tag="VHR42_02380"
     CDS             6919..7698
                     /locus_tag="VHR42_02380"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEX3026065.1"
                     /translation="MHSHSKRIEALDAIRGVAIIAMVVDHCLYDLDQIFGVPIPLLHT
                     TFFEIFRQPFTWAFILLAGVSSRFSHSNWVRGFRVLACGLVLSVVTFIYTWFTHDNES
                     IYFGILHFMGCAILLFALLRPAFEKIPPIAAFIIWSALFTVTFTMPDTYFVGIPPHFG
                     FLLPKALTNTPNLYPIGIPDANFFSADYFPMIPWFFLFMAGTVIGKPIKEHKLPEKFY
                     TAKVPFWAIAGRHTLLIYVVHQPIVYAALYLIFDVILKHHG"
     gene            7735..>8145
                     /locus_tag="VHR42_02385"
     CDS             7735..>8145
                     /locus_tag="VHR42_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3026066.1"
                     /translation="MQTILDFHTHAYPEKVAGKAVEFLNHYYHVNCEGDGTIGDLTAS
                     AKESGVSYLLVHSAATRPDQVENINTFLSQLITPNIFCFGTIHPGYPKIQDEMERLRS
                     RGLRGLKLHPDFQHFYVDTAEMDEVYEIASEFHLP"
BASE COUNT         1954 a         2068 c         1969 g         2154 t
ORIGIN      
        1 agaataattg cagcctgcct ttgcattcct ttggaaaagc tgttgagccg ctgcttcgga
       61 ttcagctcaa aaacctgcgt cagcttttga aaaagttttt cgctccaatc cggataatat
      121 ccccggtaaa atcctgccat ccggttcatg ctcgcctgtg gaaggaaata caggtcgtcc
      181 ggtacaaaaa acagacgccg ccggaccgaa gcgtcgctgc atacaagcga gccgtcaatt
      241 agcacttctc ccccatcggg ccggtaaacg ccggaaacca gcttgagcag cgtcgttttt
      301 cccgcgccgt tcgcgccgac aagcccgtaa acagaacctt tttcaatctt gcaggagcag
      361 ttttcaagca cggtaaaatc cccgaaactt ttttgaacgt tttttacctc aatcaattgg
      421 aatcctcctt tttatcataa atccggtcga gcagttcgga tacctcattc tttgaaagcc
      481 cgcttgaacg gcctaccctc agggcggttt cgatttgaac caaagccttc gacttgagca
      541 catgattttg gagggctttt tctgaaataa aactccccct gccggaaagg gtgtaaatca
      601 tccccgcgga ttccagctcg gtaaacgccc gcttgaccgt gttaacgttc agcttcagtt
      661 ccgaagccag ggaccgaatc gagggaagct ggtcgtttgg ctttaggatt tccagcgtga
      721 cgagctctga aatctgattg atgatctgct cgtaaatggg cacccgactg cgaaaatcaa
      781 tggaaatcaa taattatgac catcctttct catttcccaa ttgtctaagt gtgatatcac
      841 atgtggtaca cttattataa acagtttctt acaatatgtc aagcaaaaac gatttttcct
      901 tcgctaattt aaggataggt ctttatatta tcttgcataa aatcttttta aatcgtataa
      961 ttattttatc cttttgtgga gggatgtttt tgctgcagga tactgaagaa atccagaagc
     1021 tggtcgagca actagacggt gttacggctg ctaaaatagt agtaggctca cagggtcaaa
     1081 tgacggaaat ccatattctc gctgaccgca gcaaatcacc gaaacaactg tcccgggaca
     1141 tccagtccgc cattacggcg gccacagggg tctgtgtcga acacaaaatt atcagtatcg
     1201 cacaagtcgg cagggaagcc ctgaaacagg ctccccggct caaaattgaa aatgtggaaa
     1261 tttccagcgg cgtcgacggt ttttccgcca aagtcactct tgccgccgat cataaacagt
     1321 atgtcggatc ggcaaacggg ctgagcacgg cggcaggccg gtacaatacc gtttcgaaag
     1381 cttgcctgaa cgcggttcag gaatacagcg ccgcctgtgc cttttacgtg tcggacgtca
     1441 tcaaaataag gattgccaat accgacgaaa tcaacgttgc cataagttat tttgacaaag
     1501 atatcgaaaa aatactgacg ggggcggcac tcgtaaaaaa cgatgaatat tacggcatcg
     1561 ttcgtgcgac cctgaacgcc gtcaaccggg tcatcccggt tttatcctga taaccggaca
     1621 cgggaaagcc ggttttgatc cctgatatcg atacgggccg cggaagaagc ggagcggaaa
     1681 cagcctgtcc cattcagcga agtggacaga gaaaccttaa aaggaggctg tttccatgtt
     1741 gcgcaaagtt ttatttgccg caagcgctgt tgtgggcctt attctctcga gcggtgctta
     1801 ctggaaaatt ttcgcttaat taagaagccc cttactaaat gaactctgcg gcccgtattg
     1861 gatatagatt aacaacagct ggagaatcaa tcaaaggaca aaaaccggaa tctaaatttt
     1921 aagcagaagt tctgtatttt tcaacgttct atcataactg aatgtgccac gatttcaata
     1981 acgcttgact ttcagtctgg cgaaaaagaa tgatcatata tgcatagtaa atcagaaaaa
     2041 ttttttttgg cgaccgttat atcgtccgga atgatcctgc ttttactaac gacatatttt
     2101 tgtcccattt tggattggaa ggatttttta tttttatcaa tcctgggaat tattacggaa
     2161 tcccaagccg ttgagtttga cgacgcgaat ggaatttcga ttacaagcgc catcatcata
     2221 tgcgcgatac tgctgggaaa tccggctgtg gtgttatggg tatccgccgt ttcggtcagc
     2281 cttgcaattg tgaaaaaaca cgggggcggt ttttacagca ttttcaacat cccgtattat
     2341 gcaacgcttt ttaatctgag cttatatgtc ctgaccgcat ccgccaatat ggctgcctat
     2401 tatcttcttg gcggaaccat catccaaaaa cgatacgcgg cgttcagctc aaccgttcat
     2461 ttgatcagcc tcaactccgt tcctctgctg atttccattt tcaccggtat tttcgtaaat
     2521 accgtgctgg tggccctttt aatggcgatc cgcagtcatg aaagcatttt gaaaatatgg
     2581 tcctccaagc tgctctggtc aattgtcaat ttagttattg tcgggcttat aggagtgatt
     2641 atcacagcgc tttatgccgc ttacgactgg tttattgttt tgctgttttt tgccccgttt
     2701 acgcttgcaa gatatgtttt cgcctactat aaggatttac agcacagcta tcttcagacg
     2761 gtaaaaagcc tgaccgcagc gattgagacg aaggatgagt acacgatcgg ccactcaaaa
     2821 agagtagaat tctacagcgg cattattgca aaggaaatga agctttccgc aaggcggtgc
     2881 aattccctgc ggtacgcagc cctgcttcac gacatcggga aaatcggtat tgatgaagaa
     2941 attttaaata agaaagaaaa actttccgac gaagattgga aattgatccg gaaccacccg
     3001 gataaaggcg cccatattgt agaggatatc gaatttttat ccagctcggt ggacattatc
     3061 cgatctcacc atgaacggtt cgacgggaaa ggctatcccc ggggtttgag cagcaatgag
     3121 ctgccgctgg aagcaatgat tttggcggta gccgattcct atgacgccat gacttcggac
     3181 aggccctacc ggaaagccct cagtcaggac accgcgcttg tagagctcca gcatggagct
     3241 ggatttcagt tttcccccga agttgtccgc tcgtttgacc gcgccattca caaaaaggag
     3301 aaccagattc atgttgcttg aagccgcgat tgccggaatt gctgccgggc tgcttctgaa
     3361 agggagcctg cgctgtttgc tcgactgtaa actgcgtttt tttccgctgc tgctcgcttc
     3421 cgtacttctg agcctcctgc ctaaaatgcc ggggatcaac atcctgcttt cccggtttgg
     3481 cgcaccggcg gcatttgcgc ttgccgtcct gcggtacgga gcgctgcttg catttgtttt
     3541 tttcaaccgg ggaaaaaccg gcttgccgat tgtaggtgca ggagggtttt tgaacgctct
     3601 tgtcacgatt gcaaacgggg gaaaaatgcc tgtttcccag gccgctgttg ccgcggcccc
     3661 ggccggaaaa gatacgatcc ttttacagca gggggaaatc ctaaattatt gtattgcagg
     3721 cgcccggaca aggctcgcgt ttttatgtga ctgcatcaaa ttacgcggaa tattcctcta
     3781 ttttctcagc tttggcgaca ttctgattgc agttgggatt ttcctgtttc ttctcgctga
     3841 aatgaaaccc cgcatcctta ccgggaagcc cccctttggg aacagcaggg gaaatataga
     3901 aaacgggaaa tagtctataa tagttatcaa cgtattggcc agtccttttt aaggacggat
     3961 ggaggtttct ttgaaaagta tccggaatta tctgcaaaaa aacgttctgc ttgccgacgg
     4021 cgcgttcggc acttatattt cgtccctgct cggcaggaac atgccatcct gtgaactgct
     4081 gaacctgaaa gacccggagc ttgtactcaa tgtgcataaa caatacgtcg cggctggttc
     4141 caggctgatt ggaaccaaca ccttttccgc caatacgttt tctctcgaaa caaaccgtgc
     4201 cgaaaccgac gaaattatcc ggtcgggcgt ctcccttgcg cgaaaagcgg cgggggaaaa
     4261 cgtctacgtc gcggcggaca tcggccccgt tccgcagggc gaccgcgact cggacgcaat
     4321 ccatgaagaa tattgccgga tagccgacgc ttttctggct gaagacgtgc agatttttat
     4381 tttcgaaaca tttgccgatt ccgacgcccc cctgtttctc gcggagtata tcaagaaccg
     4441 ggtgccggac gcatttatcc ttacaaatta tacggtaact tccgacggtt attcccgtat
     4501 gaatgtcaag ggggacaaac tgatccgttc ctcggcggac agcggcttcg ccgatgcggc
     4561 cgggtttaac tgcggctgcg gccccgccca tctggttaaa tacgcttccg gcattgattt
     4621 cggcggactc ttccccgcgc tgctccccaa tgcgggctac cctcagatgg aaaatgactt
     4681 ttacctttac tccggttcgc cggaatattt cgcgagcaaa atgctggaag cagtgcagtg
     4741 cggatttaaa attttcggcg gctgctgtgg gacgacgccc gagcatatcc accgtctgtc
     4801 cctcgctctt aagaatgttt ccgggcaacg cccgataaaa aaagaatccg gcgtggagat
     4861 ttccgtcccc actgtcaggc gttctgaatt atttccggaa accggcagga aaatatttat
     4921 cgcggagctt gacccgccgt tccagtccga tcttaccaag ctggaatcct gcgccggttt
     4981 ccttcgggac aacggagtcg acgcggttac cgtcgccgac tcaccgatgg cgcgaccccg
     5041 ggtggactcg attgcggtcg ccgcgcgcct aaaacggaag ttcgggattg aagccgtgcc
     5101 gcatatcaca tgccgcgaca ggaacctcaa cgcgcttcgc tcctcactgc ttgcggcgca
     5161 catggaaaac ctgaggaaca ttctggccgt tacaggagac cccgtttccg aaacggatcg
     5221 gggctccgtc aagggcgttt ttaacctgaa ctcggcaggc ctttgcggtt ttatccggga
     5281 catgaacggg gacgccttcc aaaacgaccc gatgttctgc ggctgcgctt tcaatgtcaa
     5341 cgccaaaaac ccggaacagg agcttcacag gctcgaaaag aaaatcaatt ccggggcgac
     5401 attcctgctt tcccagccgg tctttaccgg ccgctcggtt gaaacgctcc ggaaggcgaa
     5461 aaaaatgggc gtcaaagttg ccgctggaat catcatcccc gtcagctaca gaaacgcgct
     5521 gttccttgct aacgaaatgc ccggcattac gttgccccaa caaaacgtgc tggaacggtt
     5581 ttctcccgac atgacgcgcg aacaggggga agaggcgggg ctcatggccg cgctcgaagt
     5641 tgcccggaag gcagcggatt ttgcggacgg tttttatgtc attgcgccgt ttaaccgggt
     5701 ctatctgacc gtcgagttta tgaaacggat gaagcgggaa ctccgcctgt aatattcatc
     5761 tccaaaggta taaaaatccg taccaaaatc ggtatcaaag attttgatgc ggatttttat
     5821 ctacttttca gttggatatt cggccttgtt tgtaaaatga aaaatcaacc attcagacgg
     5881 taaattgggc gcctgaattg agatttctca ggagtatccg tttttcgctt gttcttatac
     5941 ttttcgggca tatttaagat ttgaagttga gatttaacca atccggcctt cccggcattt
     6001 tgaaatatac tcaaggcaat actcgtcctt tccggaaatc gcctccgccg cgctgacaag
     6061 accgaggaga tcccgatcaa gcgggtcgag aatcgccgcg ttcagcccat tgcatacggc
     6121 ggccacggca aacgcgcggt tgattatttt ccgtttcggg aggccgaaag aaagattgga
     6181 aaggccgcat ataaccgaac attccgggta tgctttggaa agccgggaaa tcgtctccag
     6241 cgcgtcaagc ccggccgttt cgtcggcccc cagtgggcga agcatggggt caatataaat
     6301 atcttccgcc gaaacgcccc gggacgttaa tcggctgact agctcatccg ccgtggaaag
     6361 acgcgattcc aggttctccg gcgtacccgc gtcctgcatg cacagagcca caacgccgca
     6421 gcgatgctcc gccgcgagtg cggccatatt ttcaaaccgc tctttttggg tcgtaacgga
     6481 attaatgaca ttgccttccg taccggcggc ttccagtgcg gcgcgcgccg cttcgtggct
     6541 cggggtgtcg atggaaagcg caaaaccctc cgcggcaagc gtcccggcaa gccatgcgag
     6601 catttccggc tctttctcga ggaacattcc ggcgttgatg tcgataaaac gggctccgtt
     6661 ttccgtctga gcccgcgcaa gctttataat ctctccggaa tttttcgcat ccatcagggc
     6721 tttaaccgaa ggcaccgagc tgttgagctt ttcgccgatg ataatcaaat tttttcttcc
     6781 tttcaaccag atattatgtt atcatatcat tgtgacacac taattatact ttacaacagg
     6841 ttccgaatcg ttgtcaaccg gatttaccaa gggtataaat tttcttgtcg ttattttatc
     6901 ctgaaagcga ggcttttcat gcacagccac tcaaagcgaa tcgaagcttt ggacgcgatc
     6961 cgcggcgtcg ccattattgc gatggtcgtc gaccactgcc tgtacgatct cgaccagatt
     7021 ttcggcgttc cgattccgct cctgcatacg actttttttg aaattttccg tcagccgttt
     7081 acctgggcgt ttatcctgct ggcgggtgtt tccagccggt tctcccacag caactgggtt
     7141 cgcggctttc gggtgctcgc ctgcgggctt gtgcttagcg tcgtaacgtt tatttatact
     7201 tggttcacgc atgacaacga gtccatttat ttcggcattc tgcattttat gggatgtgcg
     7261 attttgttat ttgccctgct ccgccctgct tttgagaaaa ttccgcccat cgcggctttt
     7321 attatatgga gcgccctttt cacggtgacc tttacaatgc cggacaccta ttttgtcggg
     7381 atacctcccc attttggttt cctgcttccc aaagcgctga cgaatacgcc gaacttatat
     7441 ccgatcggta ttccggatgc aaatttcttt tccgcggatt attttccaat gatcccttgg
     7501 tttttcctgt ttatggcggg gacagttatc ggcaagccga tcaaggaaca taagctgccg
     7561 gaaaaatttt atacggcaaa ggttccgttt tgggccatcg ccggacggca tactttattg
     7621 atttacgttg tgcatcagcc tatcgtttac gccgctttgt atcttatttt tgacgttatc
     7681 ctgaaacatc acggataaaa gaaattttta agcttcgaaa ggaagtaact ctttttgcaa
     7741 accattcttg attttcacac acacgcttat ccggaaaaag tagcggggaa ggcagttgag
     7801 tttttaaatc attattacca cgtcaactgc gaaggtgacg ggacaatcgg cgaccttacg
     7861 gcgtccgcaa aggaatccgg cgtgagttac ctgctggtac actccgctgc tacaagaccc
     7921 gatcaagttg aaaatatcaa cactttcctg tcccaattga ttacgccgaa tattttttgc
     7981 tttgggacaa tccaccccgg atacccgaag attcaggatg agatggagcg tttgcgcagt
     8041 cgggggttgc gggggcttaa gcttcatccg gattttcagc atttttacgt tgacaccgcc
     8101 gaaatggatg aagtctatga aatcgcttcc gagtttcatc tgccg
//
LOCUS       DATALY010000077         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_277963,
            whole genome shotgun sequence.
ACCESSION   DATALY010000077 DATALY010000000
VERSION     DATALY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277963"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..977)
                     /locus_tag="VHR42_02390"
     CDS             complement(156..977)
                     /locus_tag="VHR42_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX3026067.1"
                     /translation="MSKLGGRKERIVKSREDWRKQNQVKKLRIIMTIVGIAIVISAAA
                     GAMLAWVQVNRLFAKNVTPVTPSAAPVSSAISSLPVYSNSLNLLLVNSKNPLPSSYQV
                     QVTDYNGVQVDGRIVPSLTEMMQAASAAGCPLKLTAGYVDTAKQDQLYRAEVKRLMQT
                     EKKSQVLAENQAQTTVGRGGYNESQTGLSIVLAAGNGAGGTKWVSTPQYQWLLKNSVN
                     YGFILRYAENKQDVTGMQFQPDRFRYVGKDNALKMRQLSMCLEEYSDYVGVREEG"
     gene            1215..>1846
                     /locus_tag="VHR42_02395"
     CDS             1215..>1846
                     /locus_tag="VHR42_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX3026068.1"
                     /translation="MRKTKIVCTLGPATQDEETIKQLMLAGMNVARLNFSHGTESGQK
                     EYVDKVKKLREELNLPVALLLDTKGPEIRLKEFSKPSVTLNAGDTFTLTNRDIVGDET
                     IAAVTFDRLPSEVRPGATILIDDGLIELEVNACSETDIKCTVVNGGTISAHKGINVPG
                     IHLSLPFISEKDKADIAFAVREDFDFIAASFTRTAQDIIDLRLELEKHNC"
BASE COUNT          466 a          470 c          427 g          483 t
ORIGIN      
        1 tgccacatgc gcaggtgatc gttgtctctt tatactctgg atggatactg tccttcattc
       61 ctgtcacctc tttatcctac aactacaaca taacgaagag attatatcat aggagcacat
      121 caattgcaag tcctattttt cgattatttc cggaattatc cttcttcccg gactccgaca
      181 taatccgaat attcttccag acacatggat agctgcctca tcttaagggc attgtccttt
      241 cccacgtagc ggaagcggtc cggctgaaac tgcataccgg tcacatcctg cttgttttcg
      301 gcgtaacgca ggatgaatcc gtagttgaca ctgtttttga gaagccactg gtactgcggt
      361 gtggacaccc actttgttcc tcccgccccg ttccccgcag ccagaacaat ggagaggcct
      421 gtctggctct cattatatcc gcctcggccc accgtggtct gcgcctgatt ttcagccaat
      481 acctgggatt tcttttccgt ttgcatcagc cgcttaacct ccgcgcggta aagctgatcc
      541 tgttttgccg tatccacata ccctgcggtc agcttcaatg ggcagcctgc ggcggatgca
      601 gcctgcatca tttccgtcag cgacggaaca atacgtccgt cgacctgtac gccgttataa
      661 tcggtcactt gcacctgata agaggacggc agcggatttt tggaattaac aagaagcaga
      721 ttcagggaat tgctgtagac cgggagcgac gagatcgcgg aggagaccgg agccgcgctc
      781 ggggttaccg gggttacatt ttttgcaaaa aggcggttta cctgaaccca ggccagcatt
      841 gcgccggccg cagcagaaat gacaatcgca atgcctacga tcgtcatgat aatccggagc
      901 ttctttacct gattttgctt gcgccagtcc tcgcgggatt tgacgattcg ctcttttctt
      961 ccgcccaatt tgctcatcca atcttctatt cataataaaa acataataaa agccgttttt
     1021 ttcattgtat aacttatctg aaatgattgc aacactgggt aaattttcag accaatatac
     1081 aaactgtgcc ttaaaaattg atttcaattt agatatagag tataatttta agctgggaaa
     1141 agttttataa tatgtattac gaaaagcctt tcgaggcaaa gaatcacaat ctttgagtga
     1201 aacgaggttg taacatgaga aagacaaaga ttgtctgtac ccttggtccg gcaacacagg
     1261 atgaagaaac cattaagcag cttatgcttg ccggaatgaa cgttgcccgt ctcaattttt
     1321 cacacggaac cgaatccggg cagaaagaat atgtggataa agttaagaag ctccgggaag
     1381 agctgaatct gcctgttgct ctgctgttgg acacgaaggg cccggaaatc cgcctaaaag
     1441 aattttccaa gccttccgtc acgctaaacg ccggggatac cttcacgctg accaaccggg
     1501 atattgtggg agacgagacc atcgcggctg tcacgtttga ccgccttccc tccgaggttc
     1561 gaccgggagc caccattctg attgacgacg gcttaatcga gctggaagtc aacgcatgtt
     1621 cggaaacgga cattaaatgc accgtggtaa acggcggcac tatttccgcg cacaagggga
     1681 tcaatgtgcc gggaattcac ctttccctgc cgttcatcag cgaaaaggac aaggccgata
     1741 ttgcctttgc cgttcgggag gacttcgatt ttatcgcagc ttcgtttacc cgtaccgcac
     1801 aggatattat cgatctgcgc ttggaattgg aaaaacacaa ctgcca
//
LOCUS       DATALY010000078         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_278448,
            whole genome shotgun sequence.
ACCESSION   DATALY010000078 DATALY010000000
VERSION     DATALY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278448"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VHR42_02400"
     CDS             <1..470
                     /locus_tag="VHR42_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026069.1"
                     /translation="VFKFLTLLATVTAVVLLLFFSLFLRQISFFLLIAAMGAGYGGWY
                     LFTSFNLEYEYIVTNGEMDVDRIIAQKKRKREITVKLADIEIMAPVSEKYKRDYENPN
                     FQKKIDAGSPMSKNAYFLTVRHDKLGFTRLLFEPDDRIINCAKRAAPQKVFTE"
     gene            467..844
                     /gene="acpS"
                     /locus_tag="VHR42_02405"
     CDS             467..844
                     /gene="acpS"
                     /locus_tag="VHR42_02405"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX3026070.1"
                     /translation="MIFGMGVDMVEITRIHHLLSKHTDLSRIFTNGELSGRNQGVGRA
                     GANSLAACFAAKEAFSKAIGTGIRGFSLNEVELLHDELGKPVFQLHGKALEFCRKEKL
                     LFHVSVTHTDRCATAFVVAEKEN"
     gene            915..2462
                     /locus_tag="VHR42_02410"
     CDS             915..2462
                     /locus_tag="VHR42_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX3026071.1"
                     /translation="MRILTVSQMKKAEQYATQHGVSALRLMENAGSAAARVIRESFSL
                     SGKRCTVVCGRGNNGGDGLVIARRLHDSGASVIVVFAAGLPATGNAKEMLERLRILPV
                     AIVDYSADASACLDAIGKSQIIVDAVFGTGFHGEIAPDIRRLFDQVNTRSDTGGASVF
                     AVDMPSGANADTGEVAEGCIRAHFTVTFEMPKTGHFAFPAAEFCGEVKTAKIGIPQEA
                     FSSIETSVDLLEYDDIKAAIPARAKDSNKGSYGRLFCLCGSMGMAGAARMPAAAALRS
                     GAGLVTLGVPRGIYPVVASHFDEAMIYPLRETDEGSISLSALDRILEFAEKADAVLIG
                     CGLSKNSETVSLVKELIGRLTGPVVLDADGINAMEGHIDLLRASKAELILTPHPGEMA
                     RLLGKTIPEIQGNRIRFARNLAGDIGATVALKGAYTVVASKAGRVSINPTGNPGMARG
                     GSGDILAGIIAAFAAQHFSPENAVCCGVYLHGLAGDRCAKKYSQYGMLPTDMLLEVPQ
                     IFREMSR"
     gene            2479..3018
                     /locus_tag="VHR42_02415"
     CDS             2479..3018
                     /locus_tag="VHR42_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026072.1"
                     /translation="MRKSCMIALTLLITAFSATAVSCAGSGSQLKKDAAAKMARGFSC
                     TAKVSWESNNYEVKITRPADGGCTLTFVSPSELNSLCFSAGSSGAKITFGGLGISVDT
                     SSLPQYAMYKAFSACFDSCLSPDSLSTVQRGGNTGITGKNALGRFTLTFDANLNPVML
                     EISEANLTAHFYNFQYNQP"
BASE COUNT          754 a          839 c          867 g          748 t
ORIGIN      
        1 tggttttcaa atttcttacg cttcttgcca cagtcaccgc tgtggttctg ctgctttttt
       61 tcagcttgtt tctgcggcag atctcttttt tcctgctgat tgccgctatg ggggccggct
      121 atggcggctg gtatcttttc acctctttta atttggaata cgaatatatt gtaaccaacg
      181 gcgaaatgga tgtggaccgc attatcgccc agaagaagag aaaacgggaa attaccgtaa
      241 agcttgccga tattgaaata atggctccgg tatccgaaaa atataaaaga gactatgaga
      301 atcccaactt tcagaaaaaa atcgacgccg gcagtccgat gtccaaaaac gcttattttc
      361 tgacggtcag gcacgataag ctgggtttca cccggctgct ctttgagccc gacgaccgca
      421 tcatcaattg cgcgaagcgg gcggcgcccc aaaaggtgtt taccgaatga ttttcggcat
      481 gggcgtcgac atggtcgaaa tcacgcggat tcatcatcta ttgtcaaaac acacggatct
      541 tagccggatt ttcaccaacg gggagctttc cgggcgaaat cagggtgtcg gccgcgcggg
      601 agcgaattcc ctggccgcct gttttgccgc aaaagaggcc ttctccaaag cgatcggaac
      661 aggaatccgc ggtttcagcc tcaatgaagt cgagttgctg cacgacgagc ttggaaaacc
      721 ggtctttcaa ttgcacggca aagctctgga gttctgtcgg aaagaaaaat tgctatttca
      781 tgtcagcgtt acccacacag accgatgcgc cacggcattc gtggtcgccg aaaaagaaaa
      841 ctgaccgata ctgccgcgga tggaaaaccc attccgcggt attttcctat tgcggaatgt
      901 cgaggaggat taaaatgaga attttgacgg taagccagat gaaaaaagcg gaacaatacg
      961 caacccagca cggagtttcc gccttgcggc tgatggagaa cgccggctcc gccgcggccc
     1021 gggtgatccg ggaatccttc agtctcagcg gaaaacggtg tacggtcgtc tgcggccggg
     1081 gaaacaatgg cggcgacgga ttggtcatcg cacgcaggct tcacgattcg ggtgcttctg
     1141 tcattgtcgt attcgccgcc ggccttcctg caaccggcaa cgccaaggaa atgctggaac
     1201 gtctgcgcat tctccccgtc gccatagtgg attattccgc cgacgcctcc gcctgtctgg
     1261 atgcgatcgg caaaagccaa ataattgtcg acgccgtttt tggaacgggt tttcacggcg
     1321 aaattgctcc ggacatcagg cgactctttg atcaggtgaa tacgcgttcc gataccggag
     1381 gggcctctgt tttcgcagtc gatatgccga gcggggcgaa cgccgatacc ggggaagtag
     1441 cggaaggatg catcagggcc catttcaccg ttacttttga aatgcccaaa acgggtcatt
     1501 tcgcctttcc cgccgccgaa ttctgcggtg aagtcaaaac cgcgaaaatc ggaattcctc
     1561 aagaagcgtt ctcttccatt gaaacatccg ttgatcttct ggaatacgac gatatcaaag
     1621 ccgcgatacc ggcccgggcg aaagacagca acaaaggcag ttacggccgt cttttctgcc
     1681 tgtgtggaag catggggatg gcaggcgcgg cccgtatgcc cgccgcggcg gcgctgagaa
     1741 gcggggccgg gctcgtgacg ctgggagtgc ctcgcgggat ttatccggtt gtggcctcgc
     1801 actttgacga agctatgatc tacccgctcc gtgaaacgga cgaaggctcg atttcacttt
     1861 cggctcttga ccggattctt gaatttgccg aaaaagcgga cgccgtgctg atcggctgtg
     1921 gattatcgaa aaacagcgag acggtgtcgc ttgtcaaaga gctgatcggg cggctgaccg
     1981 gccctgtcgt tttagacgcc gacggcataa atgcaatgga agggcatata gatttattgc
     2041 gtgcttccaa agcagaattg attttaactc cccatccggg cgaaatggcg cggctgctcg
     2101 gcaaaaccat tccggaaatt cagggcaacc gtatccggtt tgcccggaac cttgccggtg
     2161 atatcggagc gaccgtagcg ctcaagggcg cctataccgt cgtcgcgtca aaagcgggcc
     2221 gggtcagtat caatccaacc gggaacccgg gcatggcccg gggaggcagc ggagacattc
     2281 tagccggtat catcgcggct ttcgccgccc agcatttttc gccggagaac gccgtctgct
     2341 gcggcgtcta tcttcacggg ctggccggag accgctgcgc caaaaagtat tcccaatatg
     2401 gaatgcttcc taccgatatg cttctggagg taccgcaaat ttttcgagaa atgtctcgtt
     2461 agggagtgac cccgaaaaat gcggaaatcc tgtatgatcg cgttaacact gctgatcact
     2521 gcattctcag caaccgccgt atcctgcgcg ggcagcggtt cccagctaaa aaaagacgcg
     2581 gctgcaaaga tggcccgcgg tttcagctgc acagccaagg tcagctggga atcgaacaac
     2641 tatgaagtga aaatcacgcg gcccgctgac ggcggctgca cgttgacctt tgtcagtccc
     2701 agcgaattga attcgctttg tttcagcgcg ggcagttccg gggcgaaaat cacgttcggc
     2761 ggtttgggaa tttccgttga tacctcctca ctgccgcaat acgcgatgta taaagcgttc
     2821 tccgcctgtt ttgattcctg tctgagccct gattccctat ccaccgtaca gcgcggcggc
     2881 aacacgggga tcacagggaa aaacgcgctg ggacgattca ccctgacatt tgacgccaat
     2941 ctcaatcctg tgatgctgga aatttccgaa gcaaacctaa cggcgcattt ttataatttt
     3001 caatacaatc agccataact gaagagggct ccgggcgcca tggaccattt cagaaattaa
     3061 tttgagtaat aaacagctgt gatatttcat tttgtattat ttttcgactg ctttttccac
     3121 caggaacatc cggacggttt gaccttattc agcgtctgtg ctattcttga atcgtaaaaa
     3181 agaaaggggt gttcgcatga ataacttt
//
LOCUS       DATALY010000079         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_794921,
            whole genome shotgun sequence.
ACCESSION   DATALY010000079 DATALY010000000
VERSION     DATALY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794921"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VHR42_02420"
     CDS             complement(<1..1037)
                     /locus_tag="VHR42_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026073.1"
                     /translation="MDCWRILGIEPASDAKTIKKAYARLLKANNPEDDAAGYQRIREA
                     YDQAIAKTKKQAANKPDVVTTGGDRNPGFNFPDTSPEDQPDRTLYSSRTSDFDFSENR
                     DMPDPVFSAAEEFMNRVQALYNSFQDRIKPENWQTLLKSDCLLQLDCKMLIGNNMLLF
                     LKNQHYMPFSIIRLLIYYFEWFDREIEIEEKYPEVMTLLQMVRGPELLHYSVFLNSSL
                     PVQNLDDYFRLRELAFTSYLNNRIEEACKYAEKALRFCNYDPQLLTIAGNCFRRLFQF
                     DAALNAFTQMTRIDTKHPEGYYCCASIYYNLGDFTNALEQCRLFLNRKQTDYDGLCLA
                     ANCCRMLGDTK"
     gene            complement(1037..>1847)
                     /locus_tag="VHR42_02425"
     CDS             complement(1037..>1847)
                     /locus_tag="VHR42_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3026074.1"
                     /translation="DAIILVGGATRMQIIKTIVGKMTGKLPYTNINPDEAIALGAAIQ
                     LELKEKNQALGDMVLTDVCPYTLGIETCKHLNDERQSGYFMPIIDRNTPIPVSRAEIC
                     TTVSDYQRQIRLKVYQGESRRVENNIMIGSLEIPIPPAPAGEMPIDVRFTYDVNGLLE
                     VEVVVQKTNLKKRLVIKNTESLLSDEEIDERFKALSAIKIHPRDKAENRMLIARGERL
                     YQETTSQLRAQIDLMLQSFESVLAGQNEAQITKEREKISKIFDQIEGRDIF"
BASE COUNT          447 a          470 c          412 g          518 t
ORIGIN      
        1 tgtttggtat cgccaagcat acggcagcag tttgccgcca gacacagccc gtcataatcc
       61 gtttgcttac ggtttaagaa aagtctgcac tgctccagcg cgttcgtaaa atctccgagg
      121 ttataataaa tcgacgcgca acaataatag ccttccgggt gtttcgtatc aatccgggtc
      181 atctgcgtaa aggcattcaa agcagcgtca aactgaaaaa gtctgcggaa gcaatttccg
      241 gctatggtta aaagctgagg gtcatagttg caaaaccgaa gcgccttttc cgcatatttg
      301 caggcttctt cgatacgatt attaaggtag gacgtaaagg ccaactctcg gagtctgaaa
      361 taatcatcca gattctgaac cggcaaggaa gaattcaaaa atacggaata atgcaaaagc
      421 tcaggccctc tgaccatttg cagcaaggtc ataacttccg gatatttttc ttcaatttct
      481 atctcacggt caaaccattc aaaataataa atcagaagcc ttattatcga aaacggcata
      541 taatgctgat ttttcagaaa caggagcata ttgttcccaa tgagcatctt acagtccagc
      601 tgaagcaggc agtcgctttt cagaagagtc tgccagtttt ccggcttgat acgatcttga
      661 aaggaattgt acagggcctg cacccggttc ataaactcct ctgccgcgga aaaaaccgga
      721 tcgggcatat ccctgttttc agaaaagtcg aaatcagaag tccggcttga gtagagagtt
      781 ctatccggtt gatcctccgg cgaggtgtcc ggaaaattaa aacccggatt ccggtcgccg
      841 ccggttgtta ctacatcggg tttatttgca gcctgctttt ttgttttcgc aatcgcctgg
      901 tcgtaggcct cgcgaatacg ctgatacccc gctgcatcat cctccggatt gtttgctttt
      961 aacaatcgtg catacgcttt cttgattgtc tttgcatccg aagcaggttc gatgcccagt
     1021 atcctccagc aatccatcag aaaatatccc gtccttcaat ctggtcaaaa attttcgaga
     1081 ttttttccct ttcttttgtg atctgtgctt cgttctgccc agccagtaca ctttcaaacg
     1141 attgaagcat caggtcaatc tgcgcgcgca gctgtgaagt ggtttcctga taaagccttt
     1201 cgccgcgggc aatcagcatt cggttttccg ccttatccct cgggtgaatc ttgatagcgc
     1261 tcagcgcttt aaaccgttcg tcaatttcct cgtccgataa caggctttcc gtatttttaa
     1321 taaccagcct ttttttcagg tttgtcttct gcacgacgac ctcgacttcc aaaaggccgt
     1381 tcacatcata ggtaaaccgc acgtcgatcg gcatttctcc ggcgggagcc ggcggtatcg
     1441 gaatttccag actgccgatc atgatattgt tttcaacgcg ccggctttca ccctgataaa
     1501 ccttaagacg gatttgccgc tgataatctg acacggtcgt gcagatctcc gcccggctga
     1561 ccgggatcgg ggtattgcgg tctatgattg gcataaaata tccgctctgc ctctcgtcgt
     1621 tcaaatgctt gcaagtctct atgccaagcg tataggggca gacgtccgta agcaccatgt
     1681 cgccaagagc ctggttcttt tccttaagct ccagctgaat tgcggcgccg agcgcaatgg
     1741 cttcatcggg atttatgttc gtgtaaggca gttttccggt cattttaccg acaatcgttt
     1801 ttattatctg catccgcgta gctccgccaa ccaggataat ggcgtca
//
LOCUS       DATALY010000080         4735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_105303,
            whole genome shotgun sequence.
ACCESSION   DATALY010000080 DATALY010000000
VERSION     DATALY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105303"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..92
                     /locus_tag="VHR42_02430"
                     /pseudo
     CDS             1..92
                     /locus_tag="VHR42_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
     gene            complement(134..589)
                     /locus_tag="VHR42_02435"
                     /pseudo
     CDS             complement(134..589)
                     /locus_tag="VHR42_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain protein"
     gene            complement(638..1969)
                     /gene="glnA"
                     /locus_tag="VHR42_02440"
     CDS             complement(638..1969)
                     /gene="glnA"
                     /locus_tag="VHR42_02440"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX3026075.1"
                     /translation="MGKYSKNDVIRMIDEYNVRFIRLQFTDILGTLKNVAVTAGQMER
                     ALDNKCMFDGSSIEGFVRIEESDMYLRPDLDSFLIFPWNTQNGRVARLICDIYKPDGM
                     PFEGDPRYILKKTLQEAAERGYTYNVGPECEFFLFNTDEFGHPTTQTQDTAGYFDLGP
                     SDLGESARRDICLDLEEMGFEIEASHHEFSHAQHEIDFKYSEALLAADRLMTFKMVVK
                     TCADAHGLCATFMPKPLAGNSGSGLHVNMSLFQNDENVFYDPESKTGLSRTALSFIAG
                     VMSHIRGICAVSNPLVNSYKRLVPGFEAPCHIAWTMSNRSALIRVPAERERSTRAELR
                     NPDPACNPYLTFALLLAAGLDGIDRGLMPSEPAVANIYEMKPEEREKHGMEELPGDLH
                     AALEEMKADPMIRKVLGNHVFDKYVESKEKEWHDFSHSVTEWEISRYLSKF"
     gene            complement(2382..3620)
                     /gene="tyrS"
                     /locus_tag="VHR42_02445"
     CDS             complement(2382..3620)
                     /gene="tyrS"
                     /locus_tag="VHR42_02445"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX3026076.1"
                     /translation="MGVYEELKERGLIAQVTDEAKVRHLLDDEKTVFYVGFDPTADSL
                     HVGHFIPIMVMAHLQRAGHTPIALFGGGTGMVGDPTGKSDMRKMLTESEISHNIECFR
                     KQMSRLINFSDDKAIMANNADWLNNLNYISFLRDIGVCFSVNRMLAAECYKQRLERGL
                     SFFEMNYMIMQSYDFLELNRRYGCVMQLGGDDQWSNIIGGVELIRRKENKEAFGMTVT
                     LLTNSEGKKMGKTEKGAVWLDPEKTSPYEFYQYWRNVGDADVIQCLKILTFVPMVKIR
                     ELEKLQGEELNSAKELLAFEVTRQIHGEEEAVKARDAARALFQQGTDAANMPSADLTP
                     ADFADGSIAIPDLLVKSGLAPSRGEAKRLIAQGGITVDGEKVSDPSSAVSTEEFKKDG
                     GNYVILKKGKKAYRRVNLTE"
     misc_binding    complement(3671..3905)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3935..>4735)
                     /locus_tag="VHR42_02450"
     CDS             complement(3935..>4735)
                     /locus_tag="VHR42_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX3026077.1"
                     /translation="DPSEIDEVESRLDLLYRLGLKYGGTVNEMLAHLEEYRTEFEKIR
                     LSDENAAKFREDYKREKKRAEGLAAEISQWRKKAASDFSNRVRQELQFLDMPNVTFEV
                     HAEQVPLGPSGCDKVEFYISTNPGEPAKPIAKIASGGELSRIMLAIKTVLAGGDEVGT
                     LIFDEVDSGVSGSAAQKIGLKLREVSENRQVICVTHLAQIAALADTPYLIEKHVESGR
                     TFTQVLELNEEGRKQELARIMGGTKITPLMLQNAAEMLEMARKTPRSS"
BASE COUNT         1098 a         1347 c         1092 g         1198 t
ORIGIN      
        1 tcaccgttga gacactcagc gggattaaga ccctttctct tgcggtgcga gacggaaaag
       61 tggagtccat cgaggtcgac atgggggcgc cggtgtccat actttttttc tggataaacc
      121 gatgcgcctg tgcttcggta agatggtttc gctccatcag caaatctttg gcgcttttga
      181 ccagctgctt ttcatcgaaa ttaccgctgc tgagcttttt cttaatgttc agggacgtat
      241 aatctgagat atttaatagc atattgacgg ttgaaattaa actcatccgg ttcagtggca
      301 acatcagact gactgattca atagaatcgc acgcccccgc gaactccggg gtaacaagat
      361 agagaaagtc atacccggaa ccaaccacac gaggtaaatt gaaagccggc atatcacgca
      421 gtttaacact gcttacaact acgccgccat gataatgctt attcgcaaaa tcaatgacct
      481 gcgcacccgt cgtgcagatt cccccgatat acaggccgct tgaacggagt acgaccgcta
      541 tttttttcgc gtaatccgga ttcgaattcg ctacaataat gctgctcatt tttaacaact
      601 ccgtggaatg atgaaatatg gcccggtaca gctttcctta aaacttactg agataccggc
      661 tgatttccca ttcggtcacc gaatgggaga aatcatgcca ttccttctcc ttggattcca
      721 catacttgtc aaacacgtga tttcccagca ccttgcgaat catcgggtcc gccttcattt
      781 cttccagtgc ggcatgcagg tctcccggaa gttcctccat gccgtgtttt tcccgttcct
      841 ccggcttcat ttcgtaaata ttcgcaaccg caggctctga cggcataagg cctcggtcga
      901 tgccatcgag tcccgccgcc agcaaaagag caaacgtcag gtaaggattg cacgcaggat
      961 ccggattccg aagctcggcg cgagtgcttc gttcccgctc cgccggaacg cggatcagcg
     1021 cgctgcggtt gctcatcgtc cacgcgatat ggcacggtgc ttcaaaccct ggcaccagcc
     1081 gcttatagga gtttaccaaa ggattcgaca cggcgcagat tccccggata tggctcatga
     1141 cgcccgcaat aaagctaagt gcggtccggc tcaggccagt cttgctttca gggtcataaa
     1201 aaacattctc gtcattttgg aacagcgaca tgtttacatg aagacccgac cccgagttac
     1261 cggcaagcgg cttcggcata aaggttgcgc atagaccatg ggcatctgcg caggttttca
     1321 ccaccatttt aaaggtcatc aggcggtcgg ccgcaagaag agcctcgctg tatttaaaat
     1381 caatctcatg ctgagcgtgg gagaattcgt gatgggaagc ctcaatctca aatcccattt
     1441 cttccagatc caggcagata tcacgccgtg cactttcgcc gagatccgaa gggccaaggt
     1501 cgaaataccc ggccgtatcc tgcgtctgcg ttgtcggatg gccgaactca tccgtgttaa
     1561 agaggaaaaa ttcacattcc ggccctacgt tgtaggtata gcccctttcg gcggcctcct
     1621 gaagcgtctt tttcagaata tagcgcgggt cgccttcgaa gggcatcccg tccggtttat
     1681 aaatatcgca gatcagccgc gccacacgcc cgttctgcgt gttccacgga aaaatcagaa
     1741 aagaatccag atcggggcga agatacatat cggattcttc aatgcggaca aagccctcaa
     1801 tggaagagcc gtcgaacatg cacttattgt caagggcccg ctccatctgg cccgccgtca
     1861 ccgcaacatt ttttaaagtt ccaagaatat cggtaaactg aagccggatg aagcggacat
     1921 tgtattcatc aatcattcga atgacgtcgt tctttgaata tttccccatg ttcatccctc
     1981 cggaattttt cctgccgtcc tgaacgggaa gccttcccgc caatatttta ggaaaccgct
     2041 gatttattcc caagcgctgc gccggccggc tgatttcacg ccaaaacagg ttcaaaatgc
     2101 cccaatacag aagtatccgt gcgttttttc ctttgcctgg caaaaatctc gcggccgtcg
     2161 tcgcgctgtc cattaaatca gcggttgcaa aacgatttta ttttatttta tgctttttta
     2221 aattcagtgt caatcatttt tgatttgaaa gtccgatttc agttttatgc acaaattttg
     2281 cgcttttctg atccgaatcc cctcttctgc aggacattga acacaaaaat gcagaaccaa
     2341 gagactgaaa attcctttga ttggtccctc aggagcgttt tttactcggt aagattaacc
     2401 cggcgatacg cttttttgcc ttttttcagg atgacgtagt tccctccgtc ctttttgaac
     2461 tcttcggtgg atacggccga cgacggatcg gaaacctttt ctccgtccac ggtgattccg
     2521 ccctgggcaa tcagacgctt tgcttctcca cgggaaggag caaggccgga tttaaccaac
     2581 agatcgggaa ttgcaatgga accatccgca aagtcagcag gcgtaagatc agcggaaggc
     2641 atattcgcgg catcggttcc ctgctggaac agtgcgcgtg cggcatcccg cgccttcacg
     2701 gcttcctctt ccccatgaat ctgccgggtc acctcaaagg caagcagttc cttagcggaa
     2761 ttcaattctt ccccttgtag tttctccagc tcacggattt ttaccatggg cacaaaggta
     2821 agaattttca ggcactgaat gacatcggcg tcgccgacat tgcgccagta ctggtaaaac
     2881 tcataaggag aagttttctc cggatcaagc caaacggcac ccttttccgt tttgcccatc
     2941 tttttgccct cgctgttggt cagcagggtc accgtcatgc caaacgcttc tttgttttcc
     3001 ttacgccgga tcagttctac gccgccgata atattgctcc actggtcgtc gccgccgagc
     3061 tgcatgacgc agccataacg acggttcagt tccagaaaat cgtagctctg cataatcata
     3121 taattcattt caaagaaaga aagcccacgc tccagacgct gcttgtagca ttccgccgcc
     3181 agcatccggt tgacggaaaa gcagacgccg atatcccgca gaaagctgat gtaattcagg
     3241 ttgttcagcc agtccgcatt atttgccata atggccttat cgtcggaaaa attgatcagc
     3301 cgggacatct gcttccggaa gcattcgata ttatggctga tttccgactc cgtcagcatt
     3361 ttgcgcatat ccgactttcc cgttggatcg ccgaccatgc cggtcccgcc gccgaacagg
     3421 gcaatcggcg tgtgacccgc tctttgcaga tgcgccatga ccattatcgg aataaaatgc
     3481 ccgacgtgca ggctgtctgc cgtgggatca aaacccacgt aaaaaacggt cttctcatca
     3541 tcaagcaaat gccggacctt tgcctcatcc gttacctgcg cgatcaggcc gcgttccttc
     3601 agttcttcat agacacccat atttcttcct ccagtttcgg ggaacaaaaa agccccccgc
     3661 aaaattaatt gcagagggcg gaaaatccgc ggtaccacct cagtttgcca cagcctcacg
     3721 gcggcggcct cacgggtaca aaaaataccc acgcgctgta acgtgcgttc ccacggcaga
     3781 gcctactcca agttcaaccc ggcagctccg ggaggtattt cagcaggatg ccgcacccgc
     3841 tcacaccacc cgcgggctct ctgaaacgac cgttccaact tactttttcc catcaaagct
     3901 attatggtta tcatatattc ttttcttagt cctgtcaaga gctgcgcggg gtctttcttg
     3961 ccatttccag catttctgcg gcattctgaa gcatcagcgg cgtaattttc gtaccgccca
     4021 tgatgcgcgc aagctcctgc ttccggcctt cctcgttcag ctccaaaacc tgcgtaaagg
     4081 tgcgccctga ttccacatgc ttttcaatca agtacggagt atccgccagc gcggcgatct
     4141 gtgcaaggtg cgtcacgcag atgacctgtc ggttctccga gacttcccgg agcttcaggc
     4201 cgatcttttg cgccgcgctc ccgctcactc cggaatccac ctcatcgaaa atgagcgtcc
     4261 cgacctcatc cccaccggct aaaacggttt taatggcgag cataatccgc gaaagctcac
     4321 cgccggaggc gattttggca atcggctttg cgggttcacc gggattggtg gatatataaa
     4381 attccacttt gtcacagccg gaaggcccga gcggaacctg ctccgcatga acctcaaacg
     4441 tcacgttcgg catatccaag aactgaagtt cctgccggac ccggttggaa aaatcggaag
     4501 ccgctttttt cctccactgc gaaatttcgg cggcaagccc ttccgcgcgt tttttctccc
     4561 gcttgtagtc ttccctgaat ttcgccgcgt tttcatcgga aagccggatc ttttcaaact
     4621 cggttctgta ctcctccaaa tgggctagca tttcgttcac ggtgccaccg tattttaagc
     4681 cgagtcggta aagcagatcg agccttgact ctacctcgtc gatctcggag ggatc
//
LOCUS       DATALY010000081         5249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_105745,
            whole genome shotgun sequence.
ACCESSION   DATALY010000081 DATALY010000000
VERSION     DATALY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105745"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..265
                     /locus_tag="VHR42_02455"
                     /pseudo
     CDS             59..265
                     /locus_tag="VHR42_02455"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
     gene            285..500
                     /locus_tag="VHR42_02460"
     CDS             285..500
                     /locus_tag="VHR42_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3026078.1"
                     /translation="MPIIVNLDVMMAKRKISLSELASRVDITLPNLSVLKNNRAKAVR
                     FSTLDAICRALNCQPGDILEYRGGNRK"
     gene            497..1948
                     /locus_tag="VHR42_02465"
     CDS             497..1948
                     /locus_tag="VHR42_02465"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX3026079.1"
                     /translation="MKPNIDLSAHNPIFVIASLLLAYLFCDWILFGGTGISVTLFTGL
                     FYLTAIAYLQPKPSRLFLLTMIPPVLLSACFVLYDNLPLRFFNIIALFATASVNLFFA
                     RQEESPADSGLLSLNIIRAVVIFPFKNLSKSVLILFRNIGSAKGKNALKIAAGLVLAA
                     PAALMALALLMNSDSRFEGLVNSISGSIADSFRELLTKLFVAVILSFPLFSLLYSYRQ
                     EKACGGEALEKLRSRLRVADNLTAVTALSVLNILYLIYLVTQLSYLVPALQNVLPAEF
                     SYSSFARRGFFELVTVTMLNLAFIFLTFVFAKRQDHRLGTGCRYSLIGLSAQTLFIIA
                     TDAAKMTLYIREFGLTPLRVYTSWFMLMLAAVFLLIIIKTAASDFQFFRFAAVTVTAL
                     YLMMNFMNFDALIPRYNIARYHADSSANLDVESFARLSDSMVAEILPLRNDPRYGSEI
                     NALLKSRKKALRGAAGWKNFSIARYSAVNHLNP"
     gene            complement(1945..2454)
                     /gene="nrdG"
                     /locus_tag="VHR42_02470"
     CDS             complement(1945..2454)
                     /gene="nrdG"
                     /locus_tag="VHR42_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEX3026080.1"
                     /translation="MSKVKLAGVVRESIVDGPGIRFVVFAQGCPHHCPGCHNPQTWPF
                     EGGNETDTSKVAFEMKKNPLLSGVTLSGGEPFCQPRPMAEIARDVHTMGLNVMTYTGY
                     TYERLVELGEKDGAVRELLRQTDILVDGRFVSGLKSYDLNFRGSSNQRIIDVIRTQKE
                     HRIVEADIG"
     gene            complement(2451..4799)
                     /locus_tag="VHR42_02475"
     CDS             complement(2451..4799)
                     /locus_tag="VHR42_02475"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HEX3026081.1"
                     /translation="MITRIIKRDGREAPFNIEKIATAIYKAAQAAGGNNYSTAMDLAA
                     AVAQELEKTQKPVPKVEEIQDVVEHVLIQTGHARTAKAYILYRANRTRVRDMNTRLMK
                     TYEDLTFKSSLDVDLKRENANINGDTAMGTMLKYGSEGAKQFYDMFVLKPEHARAHRE
                     GDIHIHDLDFLTLTTTCCQIDVQKLFEKGFSTGHGFLRSPNDIASYSALACIAIQSNQ
                     NDQHGGQSIPNFDYGMAGGVRKTYARKYRENLARAFEITEGREDAADLVNRVVAEIES
                     DKGLVPELSNKNGYCDEEATRLSECFDADAVLKAQKFALKHAVLETDRAVYQAMEALI
                     HNLNTMHSRAGAQIPFSSINYGMDTSPEGRMVIKNILLTTEAGLGNGETPIFPIQIFR
                     IKQGVNYDHGDPNYDLFKLSCRVSAKRLFPNYSFIDAPFNLQYYREGHPETEMAYMGC
                     RTRVAANYYDPSREIVSSRGNLSFTSVNLPRLAIRSHGDINLFFEELDRRVDLVIDQL
                     LARFEIQAHKKVRNYPFLMGQGIWLDSEKLGPDDEVGEVLKHGTLTLGFIGLAECLKS
                     LIGEHHGQSARAQNLGLEIVAHMRRRMDEATKEHGLNFSLIATPAEGLSGRFVKMDRE
                     RYGKIEGVTDREYYTNSFHVPVYFPISAFDKIKLEAPYHELTNGGHITYVELDGDPTK
                     NIEAFEKVIRCMKESGIGYGSVNHPVDRDPVCGFTGIIGDSCPKCGRKEGEGDIPFER
                     IRRITGYLVGTLDRFNNAKRAEVADRVKHFNVETGEGGMETL"
BASE COUNT         1168 a         1394 c         1230 g         1457 t
ORIGIN      
        1 attttgattt cggcgttgag gattctcacc cgaattcgcc aaaacgaccc tttttgcttc
       61 cgcaacgccg acaactttaa aaaatgcggc attttttgcc tttgccctat ctttggtttt
      121 tttgatgaaa atgacctttt ccgcttcgat tttgactctg atctgcgccg gagcattcct
      181 gttgggcggg cttttcctta tggtcctgtc ggagttattt cagaccgccg cacgcattaa
      241 agaagaaaat gaactgacga tatgaatttt tttggggtga tactatgcct attatcgtaa
      301 atctggacgt tatgatggcc aaaaggaaaa tatctctgtc ggagcttgcc tcccgtgtgg
      361 atattacgct cccgaacctt tctgttttaa aaaacaatcg ggcgaaagcc gttcgttttt
      421 ctacgcttga cgctatttgc cgggcgctga actgccagcc cggagatatt ttggaataca
      481 gggggggaaa ccgaaaatga aacctaatat tgatttgtcc gcccacaacc cgatttttgt
      541 cattgcatcc ctgctgcttg cctatctttt ctgtgactgg atcctattcg ggggcaccgg
      601 catctcggtt acgctgttta ccggcctttt ttacctgacg gcaatcgcct atcttcagcc
      661 gaaaccaagc cggctgtttc tcctgacaat gattcctccc gtacttttgt ccgcctgctt
      721 cgttctctat gacaatctgc cattgcggtt tttcaatatc atagcgctgt ttgcgaccgc
      781 ctccgtcaac ttgttttttg cccgtcagga agagtctccg gccgattccg gattgctttc
      841 ccttaatatc atccgggctg tcgtaatttt cccttttaaa aacctatcca aaagcgtttt
      901 gattttattc cgaaatattg gatcggcaaa aggaaagaac gcccttaaaa ttgccgccgg
      961 tcttgtcttg gccgctccgg ccgctctgat ggcgctggcg ctcttgatga attcggacag
     1021 tcgctttgaa ggtctcgtca acagcatctc cggatctatt gccgacagct tccgggagct
     1081 tctgaccaaa ttatttgtcg ccgtgattct gtcgtttccg ttatttagcc tgctctattc
     1141 ctaccggcag gaaaaagcgt gcggcggtga agcattggaa aaactgcgca gccggctaag
     1201 ggtcgccgac aacctgactg ccgttacggc gttatcggtg ctgaatattc tctatttaat
     1261 ttatctcgtg actcagttat cctatctggt tccggcgctg caaaacgtgc tgccggcgga
     1321 attttcttat tcttcgttcg caagacgcgg atttttcgag cttgtaacgg tgacaatgct
     1381 gaatctggcg ttcatttttc tgacctttgt ttttgcaaaa aggcaggatc accgattggg
     1441 cacgggatgc cgttattctt tgattgggct ctccgctcaa acgctattta ttatcgcgac
     1501 ggacgccgcg aaaatgacgc tgtatatccg ggaattcggg ctgacccccc tgcgggttta
     1561 caccagctgg ttcatgctga tgctggcggc cgtctttctg ctgatcatta taaaaacggc
     1621 cgcttcggat tttcaattct tccggtttgc ggccgtcacg gtgaccgctc tgtatctgat
     1681 gatgaacttt atgaattttg acgcactgat tccccgctat aatattgcgc gctatcatgc
     1741 agattcatcg gccaatctgg atgtcgagag ctttgcccgt ctgtcggaca gcatggtagc
     1801 ggaaatcctg ccgctgagaa acgacccccg atacggcagt gaaatcaacg ccctgctcaa
     1861 atcccgaaag aaagccctgc ggggcgccgc tggatggaag aatttttcca ttgcacggta
     1921 ttcggctgtc aatcacctaa atccttagcc gatatccgcc tcaacgatcc tgtgttcctt
     1981 ttgcgtccgt atgacatcaa taattctctg gttggaagag cccctgaaat tcagatcgta
     2041 gcttttcagc ccgctgacaa aacggccgtc caccagaata tccgtctgtc tgaggagctc
     2101 cctcaccgcc ccgtcctttt caccaagctc gacgagccgc tcgtaggtat aaccggtgta
     2161 ggtcatgaca ttcaggccca tcgtatgaac gtcccgggca atctccgcca ttggcctggg
     2221 ctggcagaac ggttccccgc cgctgagggt aactcccgac agcagcgggt tttttttcat
     2281 ttcaaaagcc actttggatg tatcggtctc gtttccgccc tcaaacggcc atgtctgggg
     2341 attgtgacag cccgggcaat ggtgcgggca gccctgggca aataccacga aacgaatacc
     2401 cggcccgtcg acaattgact cacggaccac tccggccagt ttcaccttgc tcacagggtc
     2461 tccatcccgc cttccccggt ctcaacgtta aaatgcttta ctctgtctgc gacttcggcc
     2521 cgttttgcat tgttgaaacg atcaagggtt cccaccagat agccggttat tctgcggatg
     2581 cgttcaaatg gaatatcgcc ttccccttct tttctgccgc atttcgggca gctgtcacca
     2641 ataattccag tgaaaccgca gaccggatcg cggtcgacag gatgattcac cgaaccgtac
     2701 ccaattccgc tttccttcat gcagcgaatg actttttcaa aagcctcaat attctttgtg
     2761 gggtctccgt ccagctccac ataggtgatg tggccgccgt tggtcaactc atggtatggc
     2821 gcttccagtt tgattttatc aaaagcgctg atcggaaagt agactggaac atgaaaagaa
     2881 tttgtgtaat actcccggtc ggtcacccct tcgatttttc cgtaacgctc ccggtccatt
     2941 ttgacaaagc ggccggacaa cccttccgcc ggtgtcgcaa tcaacgagaa attcagcccg
     3001 tgttctttgg ttgcctcatc cattctgcgg cgcatgtggg cgacaatctc aaggccgaga
     3061 ttctgcgccc gggcggattg cccatgatgc tccccgatca gcgatttgag acattccgcc
     3121 aggccgataa acccaagggt caacgttccg tgtttgagca cttcgcccac ttcgtcgtcc
     3181 ggccccagtt tctcggaatc cagccagatt ccctgcccca tcagaaacgg ataatttcga
     3241 acctttttat gggcctgaat ctcaaaccgc gccagcagct ggtcgatgac cagatcaact
     3301 ctgcggtcaa gctcttcgaa gaaaagatta atatcaccgt ggctccgtat tgccagtctg
     3361 gggagattaa cagaggtgaa gctgagattc cccctggaac tgacgatttc tctggacggg
     3421 tcgtaataat ttgccgcaac gcgtgttcgg cagcccatat aggccatttc ggtttccgga
     3481 tgcccttccc ggtaatactg aaggttaaaa ggcgcatcaa taaacgaata gttgggaaac
     3541 agccgctttg cactcacccg gcaggataat ttgaaaagat cgtagttcgg atcgccgtgg
     3601 tcgtaattga ccccctgttt aatgcggaaa atctgaatcg gaaaaattgg cgtctcgccg
     3661 ttgcccagac ccgcttcggt tgttaagaga atatttttga ttaccatcct gccctcggga
     3721 gaagtatcca tcccgtagtt gatggaggaa aagggaatct gcgcgcctgc tctgctatgc
     3781 atggtattga gattgtgaat cagcgcctcc atggcctgat atacggcgcg gtcggtttcc
     3841 aaaaccgcat gtttgagcgc aaatttctgc gctttcagca ccgcatcggc atcaaagcat
     3901 tcagaaagcc gcgtagcttc ttcatcacaa tagccgtttt tgttggagag ctccggaacg
     3961 agccctttat cgctttcaat ctcagcgacc acacggttga caaggtcggc cgcgtcttca
     4021 cgcccctcgg tgatttcaaa agcgcgggca agattctccc tgtattttcg ggcataggtt
     4081 ttccgaacgc cgcccgccat gccgtaatca aaattcggaa tgctctggcc gccgtgctga
     4141 tcattctgat tggattgaat cgcgatgcag gcgagcgcgg agtagctggc aatatcattt
     4201 gggctgcgca gaaatccgtg accggtggag aaacccttct caaacagctt ttgaacgtcg
     4261 atctggcagc aggtggtggt cagcgtaagg aaatccagat catggatatg gatatcgcct
     4321 tctctgtgcg ctctcgcgtg ttcaggctta agaacaaaca tgtcataaaa ctgtttggcg
     4381 ccttccgagc cgtatttcag catggttccc atggcggtat ccccgttaat gttggcattt
     4441 tcgcgtttga gatcaacatc aagcgatgac ttaaaggtca ggtcttcata agttttcatc
     4501 agacgggtat tcatatcccg aacacgggta cggttggcgc gatatagaat ataggctttg
     4561 gctgtgcgcg catgacctgt ctgaatcagc acatgctcga caacatcctg tatttcttca
     4621 actttaggaa ccggtttctg ggtcttctcc agctcctggg ccacggctgc cgccagatcc
     4681 atcgcggtgg aataattgtt gcccccggcc gcctgcgcag ccttataaat cgcggtagcg
     4741 attttctcaa tgttaaaggg agcctcccgc ccgtcccttt taatgattcg tgtaatcatt
     4801 taaagccact tcctatcctg ttttcttatc tttataccat atatagtgta acgtattagt
     4861 cgcggtacat gatgatgggt acattatata ccacaatttt caatttttca accctgattt
     4921 ataaaaatca tctggctcat tcgtccgccc ggcgcgccac gatgaactta tacagccggg
     4981 aacaaaccat attatttttt tctgattgtt ctattgagta tttcagacaa tctgaggaag
     5041 aataaatatt gattatgcaa tcggctaagt aaaaaacagg agagcaacca tgaaaccatc
     5101 atcagattca cagtttgaat ccattctcag tgaaaatcgc cttcatacac tgttccagcc
     5161 catcgtcaat ttcaaagacg gaaaaacagc tggatttgag gcattgagcc gcgggcctga
     5221 tcacacggcg tatttttctc cgctctttc
//
LOCUS       DATALY010000082         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_451385,
            whole genome shotgun sequence.
ACCESSION   DATALY010000082 DATALY010000000
VERSION     DATALY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451385"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..571
                     /gene="nifU"
                     /locus_tag="VHR42_02480"
     CDS             125..571
                     /gene="nifU"
                     /locus_tag="VHR42_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HEX3026082.1"
                     /translation="MAYSQKVMDHFANPRNTGEIPDANGVGEVGNAVCGDIMKMYLKI
                     KDDVIEDVRFKTFGCGAAVATSSMATELIKGKSIQDALKLTNKAVMEALDGLPPAKIH
                     CSVLAEQAVKAAVSDYYRRQGIDPEPIVGKLENCEACHLHQPNEKA"
     gene            568..1275
                     /locus_tag="VHR42_02485"
     CDS             568..1275
                     /locus_tag="VHR42_02485"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX3026083.1"
                     /translation="MTGRRNNPNRILKHILAVSLVCVLALGCSFTLYALFSAGRTNKT
                     VSAASVAGSAMPADSSSVSAVSSSSVSSVSSASSAASSSLQTSSAVSKSASSVSSVTG
                     GAAAVSNAAKPVWILVDIAAQRTTVYDADNRVIKKFVCSTGAPGTDTPTGTFHIQARG
                     KSFFSQKYQEGGYYWMQFYGNYWFHSIPFDKERIIIPTEAVKLGEKASHGCVRLAIDD
                     EKWMYNNIPKGTKVVIK"
     gene            1446..2711
                     /locus_tag="VHR42_02490"
     CDS             1446..2711
                     /locus_tag="VHR42_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026084.1"
                     /translation="MRAEKIKAQPFDCMQYFYTEVQEPRIRCFIRFGDRISETALNRA
                     VGLSIHAVPLIGCVFNEKRHCWEKRSFTADDIVHFVEAPAADEIPVPDCLLCSIDPAR
                     EPQLKIFLIRGKRNDSLCFIINHMICDGAGFKDYLYLLCNLYTKCEIDAGYNRKPEPF
                     GKRGLGQLLKNLSFKQKLEILLSKPHYNKPDPAMIMPIKGGADKPMIVVAKIEKEQFA
                     AIRARAKSRQVSVNDALLTAYIRAVHRATGCREITIPCPVNLRKYRKAEQRCGICNLT
                     ANYICSVDVDPEEPVDVTLRKVSVRMRAQKESNECLKKPMLFHITFHLLPFKTMWAIF
                     RRNSPVPVTSYTNIGVLDDGRLHFGAHNAEDAFISTAVKKHPYFQLSVSTYNGSCTLT
                     SSLYGTKEDRGFIENCFGRIIEELVLLPQ"
     gene            2921..3411
                     /gene="tnpA"
                     /locus_tag="VHR42_02495"
                     /pseudo
     CDS             2921..3411
                     /gene="tnpA"
                     /locus_tag="VHR42_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          972 a          842 c          892 g          805 t
ORIGIN      
        1 atcgattata ttctggaagt tctgcccggt atcgtaaacc gtctgcggga gatgtccccc
       61 gtatgggaaa agatgcagag cgggaagtaa agaaaccggt ttttctgaaa ggagaataag
      121 taatatggca tacagtcaga aagtcatgga tcattttgcc aacccccgca atacgggcga
      181 gattcccgat gcgaacggcg tcggggaagt cggcaacgcc gtatgcggcg atatcatgaa
      241 aatgtaccta aaaatcaagg acgacgtgat cgaagacgtc cgtttcaaaa cattcggctg
      301 cggagcggct gttgcaacct cctccatggc gacggagctc atcaagggaa aatcaattca
      361 ggatgcgctg aagcttacca ataaagccgt tatggaggcg ctggacgggc tgccccccgc
      421 aaagatacac tgttccgtgc ttgcggagca ggctgttaag gccgcagttt cggattatta
      481 ccgccggcag gggatcgacc cggagccgat cgtcggtaag ctggaaaatt gtgaagcctg
      541 ccatctgcat cagccgaatg aaaaagcatg acaggccgaa gaaataatcc caaccggatt
      601 ttaaaacata ttcttgccgt ttcgcttgtc tgcgttttgg ctctcggctg ttcctttacc
      661 ctgtacgcgc tgttttccgc cggacggacg aacaaaacgg tttccgccgc aagcgtcgcc
      721 ggatcagcaa tgccggccga ttcatcgtcc gtttccgctg tttcctcgtc ttccgtctcc
      781 tccgtatcct ctgcatcttc cgcagcatcc tccagcctgc agacgtcttc ggccgtttca
      841 aagtccgcct cctccgtttc ttccgttacc ggcggagccg cagccgtttc gaatgcggca
      901 aaaccggttt ggattctggt ggacatcgct gcccagagga caaccgtcta cgacgctgac
      961 aaccgcgtta ttaaaaagtt tgtttgctca accggagcgc ccggcaccga tacgccgaca
     1021 ggcaccttcc atattcaggc gcggggcaaa tcctttttca gccagaaata ccaggaaggc
     1081 ggctattact ggatgcagtt ttacgggaac tactggttcc acagcatccc cttcgataaa
     1141 gaaagaatca ttattcccac cgaagcggtg aagctggggg aaaaggcgtc gcacggctgc
     1201 gtacggctcg cgattgacga tgaaaaatgg atgtacaata atattcccaa aggcacaaag
     1261 gtagtcataa aataacagaa gtcccggccg ctcaggcggc tgggactttt ttaactgccg
     1321 aatcaaaccg gattgacaaa aattagaacc catcgtaaaa tagcgttgat gaaacggcaa
     1381 aaaaacgggc aggcagagga accgcaaaca gcgttttcct gaggcggtga aaggggattt
     1441 cgaggatgag ggccgaaaag atcaaagcgc agccttttga ttgtatgcag tatttttaca
     1501 cagaggttca ggaaccgcgt atccgctgct ttatccggtt cggggatcgt ataagcgaaa
     1561 cggccctcaa ccgggcggtc ggtttatcaa tccatgcggt gcccctgatt ggctgcgtct
     1621 ttaacgaaaa gcgccattgc tgggaaaaac gttcctttac cgccgacgat atcgttcatt
     1681 ttgttgaggc tccggcagcg gatgaaattc ccgttccgga ctgcctgctt tgctcgattg
     1741 atcccgcccg cgagccacag ctgaaaatat tcctcataag gggaaaaagg aacgattcgc
     1801 tctgctttat cattaaccat atgatctgcg acggagcggg cttcaaggat tatctgtatc
     1861 tgctctgcaa tctgtatacc aaatgcgaaa tagacgcggg gtataacaga aagccggaac
     1921 cgttcggcaa gcgcggcctt ggccagcttt taaaaaatct gagcttcaaa caaaagcttg
     1981 agatactgct gtcaaagccg cattacaata aacccgatcc cgccatgatt atgccaataa
     2041 aaggtggagc cgataagccg atgatcgtcg ttgcgaagat agaaaaggaa cagttcgccg
     2101 ctatacgggc gcgtgccaaa agcaggcagg tgagcgtaaa cgacgctctg ctcacggcct
     2161 atatccgcgc cgttcaccgg gcgacgggat gcagggaaat tacgattccg tgccctgtca
     2221 atctgaggaa atacaggaag gcggagcagc ggtgcggaat ctgcaatctg accgccaatt
     2281 atatttgcag cgtcgacgtc gatcccgaag agccggttga cgtcactctt cggaaggtaa
     2341 gcgtccggat gcgggctcaa aaagaaagca acgaatgctt gaaaaagccg atgctgtttc
     2401 acataacgtt ccatctgctc ccgtttaaaa cgatgtgggc cattttccgc aggaattcac
     2461 ccgtgcccgt tacttcctac accaatatcg gcgttctcga cgacggcagg ttgcatttcg
     2521 gggcgcacaa tgcggaggat gccttcatca gcacggccgt taaaaaacac ccttattttc
     2581 aactttccgt ttcaacttat aacggaagct gtacgctgac gagcagcctg tacggaacaa
     2641 aagaagacag gggctttata gaaaactgtt ttggccggat tatagaggaa cttgttttgt
     2701 tgccgcaata aaacggcgag tttacacaaa ggtcttgaac aaaaattaaa acccaccgac
     2761 ggcttgaaac cgtcggtggg tttattacgt tgtagcttta gcgcaaataa ccaccggctc
     2821 tgccggtgtg gataaagaat ctttagatac aagaaaacct ccttttgcta aaatagtagt
     2881 aggttcacca acagcactaa aagcaaaagg aggttttgtc atgactgaca taaacagttt
     2941 atcacattcc aaatggagat gccaatacca tatagtgttt gcgccgaagt ataggcgcaa
     3001 agagatatta ggcgcaaaga gatatatgga aagataaagg cggatatcgg agaaatccta
     3061 cgaaaattat gtgaacagaa aggcgtagaa atcattgagg cacaggcatg ccctgaccac
     3121 atacatatgc tggtgagtgt accaccgaat ataagcatat cacaatttat agggtttctt
     3181 aatggtaaaa gcagcttgat gatttttgac tgacatgcca atctgaagta caaatacgga
     3241 agtcggcatt tctggtgtag agggtactac gtagatacag ttggtcgtaa taaaactgca
     3301 attgcagaat acatcaagaa tcagttgtag gaagatattg tagcagatca aatttcactc
     3361 aaggaatact tagacccgtt tacgggtagc aagaacacag aggcaaaata acaggccgct
     3421 ttagcggcgg cctgtaaacg taatgcggtt ggcgaatctt tcagtgcctc ttaagaggct
     3481 gccagtaata tgcccttcta gggcttgtgc a
//
LOCUS       DATALY010000083         5367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_623445,
            whole genome shotgun sequence.
ACCESSION   DATALY010000083 DATALY010000000
VERSION     DATALY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623445"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VHR42_02500"
     CDS             <1..391
                     /locus_tag="VHR42_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX3026085.1"
                     /translation="VGDCGRNVGTLMNIAGAEQFNIVDIPLRTFGNFCVCKNEAEALH
                     NYKEGDILVIPETSNDLLPLLKSAAAIITEREGKNSHAAVVGLTLDKPVIIGAPNATL
                     LLKSGTAVTVDASRGIVFSGVEKLPVG"
     gene            499..690
                     /locus_tag="VHR42_02505"
     CDS             499..690
                     /locus_tag="VHR42_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX3026086.1"
                     /translation="MKAKLIRHNCIGCGLCGSICPEVFRTAEDGYAKIIRESVPKEAE
                     HSAVEASDECPVGIIIIEK"
     gene            complement(753..2093)
                     /locus_tag="VHR42_02510"
     CDS             complement(753..2093)
                     /locus_tag="VHR42_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX3026087.1"
                     /translation="MNQANCPARYSEVQDRLSGGTNFRQFHAQARGMIRSFPDQHFFI
                     VSYHVEQFRFINELFGYENGTKVLRGVAKVLRGVILERELCSHVYADRFCLLMNEKTP
                     EQLTERLLRIFDLLKDTIEFYGIHYNMISRFGVFDCGQKRTLGIPQMINRAVIAEKLP
                     TDISSTVSFYDESMSTAQMTEKNKEDRLLPALEKHEFEIYYQPKYQVGDRSIAGAEAL
                     VRWQTERNTLVLPDEFVPTFENSGTSPLLDAYVMENVCVDIRRWMDHGAKLLPIAVNL
                     SRVSLQAEGLAEKYRKTADRHGVPPRLIELEITERAFSECSAEQRKTVEKLHTAGFAI
                     AIDDFGAGCSSLCMLRDIPADTVKLDRIFLKDVQHTEKGRIVAASMIELARRLNLKVV
                     AEGVESEEQMVFLCENRCDIAQGFHLSKPIPASEYQRLLQIKNHGGTQPDIKSG"
     gene            2355..3650
                     /locus_tag="VHR42_02515"
     CDS             2355..3650
                     /locus_tag="VHR42_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3026088.1"
                     /translation="MKTLLMLSFPTLMMSVIQSLLPLSDGLFINNAAGTLVASAVTFS
                     EPVVNMMIALAQGISVAAMAIIGQANGQGDFLRVRHISTQIIVFSSCVGVCIAPLLAL
                     IAIPVSAGVDPEISHNVFLYISLNAMVLPFSFLENIYNAIKNATGRPEATFVRMIIML
                     ALKIMFNSIFIMHFRMGIYGCVLSSLCANILICIWMFHELFIKKGDDRLTLTGFRLDR
                     AIIKQTVRVGIPAMLMSLMLNLGFFLINHETQKYGAVVLNGQGIANNISAVCFNLPGA
                     FGSAVTTMVSFNIGAQQPLKARKSCYIGCAASAVTAAAIIAIIVPASPFLTVLFTQNP
                     NVLEISNHALHIYTYSVIGFGVCMAVQGAFIGLGKTSIPMVLGILRIWFLRYIFILAT
                     EHVLKYYSVFWGNLFSNYMAAIIALILISRVRWVSVLHS"
     gene            complement(3723..4082)
                     /locus_tag="VHR42_02520"
     CDS             complement(3723..4082)
                     /locus_tag="VHR42_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX3026089.1"
                     /translation="MGCLGNTVWFLLGGAIMGISWAVVGVLWCLTIVGIPVGVQCFKL
                     AELAFFPFGRQIVFEGGAGSVLLNLLWLIFGGLALAAEAAAVGLLFCITVVGIPFGLQ
                     CFKIAKLALMPFGARVL"
     gene            complement(4156..>5367)
                     /locus_tag="VHR42_02525"
     CDS             complement(4156..>5367)
                     /locus_tag="VHR42_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3026090.1"
                     /translation="IDRDSMKYALVKSMVELSRMANIQLIAEGVETSDELATLINLGV
                     QFAQGYFIQRPQSEILLPVPEVLSRIIELNRRRNHVYGNQVGNIYIENICTPTETITP
                     QAKVDEVFSKLKGDPFAFGFCVVENDAVVGVATKTHLVLQLSGLYGYSLNQNRRISAL
                     MDSNFMAVDCQTPINVVSNAAMSRQPDKLYDFIVVTKGGKYLGTVTIKDLLQKTTEIE
                     VINAKGQNPLTGLPGNIAIERKMQQMIQSEQAYSAVYFDIDNFKAYNDVYGFENGDKI
                     ICLLADILVANLPQNQFVGHVGGDDFVVIQQGNDPQMYCRRVMESFQSMVMKYYSPED
                     AARGFILAENRRGEMEQFPVVSISAAGVIDAGNTFTDVEGLTETLAKLKKKSKLTKGN
                     SSCWKTVGTAV"
BASE COUNT         1340 a         1446 c         1242 g         1339 t
ORIGIN      
        1 agtgggagat tgcggccgta acgttgggac gctcatgaat attgcgggtg cggaacagtt
       61 caatatagtt gatatcccgc tccgtacgtt cggcaatttt tgcgtttgca aaaatgaagc
      121 ggaagcactg cacaattata aggaaggcga cattctcgtc attcccgaaa cctccaacga
      181 tcttctccct cttttaaaat cggccgccgc catcatcacg gaacgggaag gcaaaaattc
      241 gcacgccgcc gtggtgggac tgactttaga taaaccggtc attatcggag caccgaatgc
      301 aactctttta ctgaaaagcg gtaccgctgt cacagtagac gcatcccgtg ggatcgtatt
      361 cagcggtgtg gaaaagctcc cggtcggtta agcttctcaa aaattccgct catttccttt
      421 cggaatgatt cctaaagcgg aagggaatac tttttgaacc aacggatttc cgttttcgaa
      481 cgggaaaggt gtgttgccat gaaagcgaaa ctcatccggc acaattgcat cggatgcgga
      541 ctttgcggat cgatctgccc agaggtattc cgaacggcgg aggacggcta cgcaaaaata
      601 atccgcgaat ccgttccgaa agaagcggag cattcggccg tagaggcaag cgacgaatgc
      661 ccggtcggaa tcataattat tgaaaaatag cactgtcata aacaatgcgt tctgaacggg
      721 taagaccgat tcaggacgca ttgttttttt acttatccgc tttttatatc cggctgagtg
      781 ccgccgtggt tttttatctg cagcagtcgc tgatattccg aagcggggat cggctttgaa
      841 agatggaagc cctgcgcaat atcgcaccgg ttttcgcata gaaaaaccat ctgttcttcc
      901 gattccactc cctccgccac gaccttgaga ttcagccgcc ttgcaagctc gatcatagaa
      961 gccgccacga tcctcccctt ttccgtatgc tgaacatcct tcagaaagat ccggtccagc
     1021 tttacggtat cagcgggaat gtccctgagc atacacagag aagaacagcc cgccccgaaa
     1081 tcgtcaatgg caatcgcaaa accggcggta tgcagctttt ccacggtttt gcgctgttct
     1141 gccgagcact cggaaaaagc acgctccgtg atctccagtt caatcaaacg gggaggaacg
     1201 ccatgccgat ccgctgtctt gcggtacttt tcggcaagtc cctccgcctg taagctgacg
     1261 cgggaaagat tcaccgcaat tggaagcaac tttgccccat ggtccatcca acggcggatg
     1321 tccacgcaga cgttttccat cacataggca tccagcagcg gggaagttcc gctgttttca
     1381 aatgtgggca caaattcatc tggcaggaca agcgtgtttc gttccgtctg ccagcggacc
     1441 agcgcttccg cgccggcgat cgaccggtct cccacctgat atttaggctg atagtaaatc
     1501 tcgaactcat gcttttcaag agcgggcagc agacgatctt ccttgttctt ttccgtcatc
     1561 tgggccgtgc tcatgctctc gtcgtaaaac gaaacggtac tggagatgtc tgtcggcaac
     1621 ttttcggcaa tcaccgcacg attgatcatt tgcggaatgc cgagcgtgcg cttttgtccg
     1681 caatcaaaca cgccgaaacg ggaaatcatg ttgtagtgaa tcccgtaaaa ctcaattgtg
     1741 tcctttaaaa gatcaaaaat ccgcagtagc cgttctgtaa gctgctcggg ggttttctca
     1801 ttcatcagaa ggcaaaaccg atccgcatac acatgggagc aaagctcccg ctccagaata
     1861 acaccccgaa ggaccttggc gacaccgcgc aataccttcg taccgttttc atagccgaac
     1921 agttcgttga taaaacggaa ctgttccaca tgataggaaa cgataaaaaa atgctggtcc
     1981 ggaaacgatc ggatcatgcc tcgtgcctga gcatggaact gccgaaaatt tgtgcctccg
     2041 cttaaacgat cctgaacctc cgagtacctt gccgggcaat tcgcctgatt catcgattcg
     2101 gacccctttc cggataaaat cataagctta atttcatctg gaatcaggaa atttatacgc
     2161 gcgtgccagg gtttcagaac gaatatttcc attttttcaa cggtcagcat tctttctaaa
     2221 gcgattccgt tcttgaaaaa gggaaatcaa ggtgataaaa tggtccacat atggaaagcg
     2281 gggttagctt gaatttagaa tttttacacc aaagtacaga acaaagaaga gacattattc
     2341 ttcacggaag catcttgaag actttgctga tgctctcgtt cccaacgctg atgatgagcg
     2401 tgatccaatc tcttcttcca ctatcagacg gactttttat caacaacgct gctggaaccc
     2461 ttgttgcgag cgctgtcacg ttcagcgaac cggtcgtcaa tatgatgatc gcactggctc
     2521 agggaatcag tgtcgccgcg atggcaatta tcggccaggc caacggtcag ggggactttt
     2581 tgagagtccg ccacatctcc acacagatta ttgttttttc ttcctgtgtc ggggtctgca
     2641 ttgcgccgct gcttgcgttg atcgccattc cagtgtcggc tggcgtagac ccggaaattt
     2701 cccacaacgt gttcctgtac ataagcttga acgccatggt cctccctttt tcctttctgg
     2761 aaaacattta taatgccatt aaaaatgcca cgggcaggcc tgaggcaacc tttgtgcgca
     2821 tgattattat gctggcgctt aaaattatgt tcaacagcat ctttatcatg cattttcgca
     2881 tgggcatcta cggctgtgtg ctttcttccc tttgcgccaa tattttgatc tgcatctgga
     2941 tgttccacga actttttatt aaaaagggcg atgaccggct gactctcacc gggttccgcc
     3001 ttgatcgggc gattatcaaa caaaccgtcc gcgtcggaat tccggccatg ctgatgagcc
     3061 tgatgctgaa cctcgggttc ttcctcatca accacgagac gcagaaatac ggtgccgtgg
     3121 tgctgaacgg ccaaggaatt gcaaacaaca tcagcgccgt ctgctttaac ctgcccggcg
     3181 ccttcggctc cgctgtgaca acgatggtga gcttcaacat tggggcgcaa cagccgctca
     3241 aagcccggaa atcctgctat atcggctgtg cggcaagcgc cgtgaccgcc gcagcgatca
     3301 ttgcgattat tgtgcccgct tcaccctttc tgaccgttct gttcacgcag aacccaaacg
     3361 tccttgaaat ctccaatcac gcgctgcaca tctacaccta ttccgtcatc gggtttggcg
     3421 tttgcatggc cgttcagggc gcattcatcg gcctcggaaa aaccagtatc cccatggtac
     3481 tgggaattct gcgaatctgg ttcctgcgct acatattcat tctggcaacg gaacatgtgc
     3541 tgaagtatta ttccgtattc tggggaaatc tcttttccaa ctacatggcg gccatcatcg
     3601 ccctgatttt aatttcaagg gtccggtggg tctccgttct ccattcgtaa ccgaccgtgt
     3661 taaagacgaa agaaaaagcc tgtttgcaga aacgaatctc tttctgcaaa caggctttgc
     3721 atttatagaa ctctggctcc gaacggcatc agagcaagct ttgcgatttt aaagcactgc
     3781 agcccgaatg ggatacccac aacggtgatg cagaacagaa gtcccacagc ggcagcctcc
     3841 gctgcaaggg caagcccgcc gaagatcagc cacagcagat ttaaaagaac ggaaccggcc
     3901 ccgccctcaa agacaatctg cctgccgaac ggaaaaaagg caagttccgc aagtttaaaa
     3961 cactgaacac cgacgggaat tcccacaatt gtaaggcacc acaaaacacc cacaaccgcc
     4021 caactgattc ccataattgc gccgcccaaa aggaaccaga ccgtattgcc caaacagccc
     4081 atcgttttct ctccctatac cgtttctgat tatgactatt atagcataaa tccgggataa
     4141 ggaacactga acgaatcaaa ccgctgtccc caccgtcttc cagcagctgc tgttgccctt
     4201 agtcagtttg ctttttttct tcagcttcgc aagagtttcc gtaagtccct caacatcggt
     4261 aaaggtattc cccgcatcga tcactccggc agcggaaatt gagacaaccg gaaactgctc
     4321 catttctcca cgcctgttct ccgcaaggat aaagccgcgg gccgcgtcct cgggcgaata
     4381 atacttcata accatactct ggaagctctc cataaccctt cggcaataca tctgcggatc
     4441 gttcccctgc tgaatcacga caaaatcgtc gccgccaacg tgtcccacga actggttctg
     4501 cggcagattc gccaccagaa tatccgcaag cagacagatg attttatcgc cgttttcaaa
     4561 tccgtagacg tcgttgtacg ccttaaaatt atcgatatca aaatagacgg cgctgtaagc
     4621 ctgctccgac tgaatcatct gctgcatttt ccgttcgatc gcgatattgc cgggcagacc
     4681 ggtcaaagga ttctggcctt tcgcgttgat gacctcgatt tcagtcgtct tctgcaagag
     4741 atctttaatg gttacggttc cgaggtactt tcctcctttg gtgacgacaa taaaatcgta
     4801 cagcttatcc ggctgccttg acatagcggc gtttgaaaca acattgatgg gtgtctgaca
     4861 gtccacagcc ataaagttac tgtccatgag ggcggaaatt ctccggtttt ggtttaacga
     4921 atagccgtaa agtccggaaa gctgcaaaac caaatgggtt tttgtagcga ctcccacaac
     4981 cgcatcgttt tcgacaacac aaaaaccgaa ggcaaacggg tcccccttca gtttgctgaa
     5041 aacctcgtcc actttagcct gcggggtaat ggtttccgtt ggagtgcaga tgttttctat
     5101 gtaaatattt ccgacctgat tgccgtaaac gtgatttctc ctgcggttga gctcaataat
     5161 ccgactcaga acctccggaa caggcagcaa tatctccgac tgcggtcgct gaataaaata
     5221 gccctgcgca aactgaacac caagattaat cagggttgcc agctcatcgc tggtttcgac
     5281 gccttcagcg atcagttgga tgttggccat gcgggacagt tccaccatgc ttttaaccag
     5341 cgcgtatttc atggaatcgc ggtcaat
//
LOCUS       DATALY010000084         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_709241,
            whole genome shotgun sequence.
ACCESSION   DATALY010000084 DATALY010000000
VERSION     DATALY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709241"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VHR42_02530"
     CDS             <1..722
                     /locus_tag="VHR42_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3026091.1"
                     /translation="QRSFTQITGIPLSEYIRSRKLSRAAYDLQNTDDKIITIAMRYGY
                     ESADAFSAAFRKMHGVSPAAVRKSSVKLTFYCRISFSLTIKGVYKMDYQITKRGAFKV
                     IGIRRTTPYGGGTWAVVKSDGSNEAIQKLSGHFFDLGLCFGFQEDGSNDYMCGVLWEG
                     EDAAGFDSYTYQPAEWILFEAKGKISDQVLNKTWYHINNEFLPQSRYQKSGQPTIEKY
                     VQWDDAADSCDVEIWIPVTVK"
     gene            1043..1501
                     /locus_tag="VHR42_02535"
     CDS             1043..1501
                     /locus_tag="VHR42_02535"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEX3026092.1"
                     /translation="MEFTDKKRDSFRLCGFFRETSLETCGDDLHRLWEYYGSRKHELF
                     ELFGKRKDFYGLMWKTTGSRYCYLIGIETAGEEKVPEGAVTKTIPAADYAVAPVPVNV
                     SAFDAWTEFYYKALPAAGYTPNAGHGFDFEYYPNGDGAAYELWTPVTKTV"
     gene            complement(1606..>1855)
                     /locus_tag="VHR42_02540"
     CDS             complement(1606..>1855)
                     /locus_tag="VHR42_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026093.1"
                     /translation="EQKVIQTVLDSFSRLVIRDGKVDLFAMGIHGPSSTRTYLVSDGI
                     EDIGMTVKGEFASAVNPMLYLFLLYAKLFHLRKNRSTQ"
BASE COUNT          502 a          428 c          475 g          450 t
ORIGIN      
        1 tccagaggtc ttttacccag atcacaggca ttcccctgtc ggaatatatc cgcagcagaa
       61 aacttagccg tgcggcctac gatttgcaga ataccgacga taagattatt acgattgcca
      121 tgcggtacgg ttatgagtcg gcggatgcat tcagcgcggc tttcagaaaa atgcacggag
      181 tttccccggc agcagtgcga aaatccagtg tgaagttaac attctactgc cgcatttctt
      241 tttcccttac gataaagggg gtctacaaaa tggattatca gatcacaaag cgtggagcgt
      301 tcaaggttat cgggattcgg cgcacgacac cttacggcgg cggaacatgg gcagtggtga
      361 aaagcgacgg cagcaacgaa gccattcaaa agctgagcgg tcatttcttt gatctcggct
      421 tgtgcttcgg ttttcaggag gacggcagca acgactatat gtgcggtgtc ctctgggaag
      481 gggaagacgc tgccggtttt gacagctaca cctatcagcc tgccgagtgg attttatttg
      541 aggcaaaagg caaaatttcc gaccaggtgc tcaataaaac gtggtaccac atcaataacg
      601 aattcctgcc gcagagccga taccagaagt ccggacagcc gacgattgaa aaatatgttc
      661 agtgggatga cgcggcggat tcctgcgacg tagaaatctg gataccggtc acggtgaaat
      721 gaaacaacgg actgctgctt ttcggcggca gtccgtttgt attgaaactg ttcggcctgc
      781 cgattcccga ctaagcaaag ctattttgct ctcagacgta cgatcatgat gatgggaata
      841 ctttgcccca ggaatggaac ttcgtaaaaa ccatccacgg aaaatccgct tcggaacgca
      901 gtatggaaga tttcctgaat ggagcggtga taataattct gcagaacggg ctgcccgcgg
      961 actgcctctc ccttgttgat ctggcttgta aaatagtctc cggaggggta atccgggtag
     1021 gtaaagcagg gagtgagatt gtatggaatt tacagacaaa aagagagatt cgttccgcct
     1081 gtgcggtttt ttccgggaaa cgagtctgga aacctgtggt gacgatctgc accgtctgtg
     1141 ggagtactat ggcagccgaa agcatgaact ttttgaactg tttggtaagc ggaaggattt
     1201 ttacggattg atgtggaaaa caaccggcag ccggtattgc tatctgattg gaattgaaac
     1261 ggctggtgag gaaaaagtac cggaaggtgc cgttaccaaa acgattcccg ccgcagacta
     1321 tgcggttgcc cccgtccctg taaatgtttc cgcatttgac gcatggacgg aattctatta
     1381 caaagccctt cccgcggcgg gctacacgcc aaacgctggg cacggtttcg atttcgaata
     1441 ttatccgaac ggtgacggag cagcttatga actctggacg cctgttacaa aaacagtctg
     1501 accgtgagaa aaatcgggca tggaaaattc catgcccggt tttcggttta aagtgtatta
     1561 cagcaacccg caaacctttc tgcctgttgg aattgattct gaattctatt gggtcgatct
     1621 attttttctc agatgaaaca gtttggcata taataaaaac aaatataaca tcggattgac
     1681 agcactggca aactctccct taaccgtcat accaatgtcc tcgataccat cgctgacaag
     1741 atatgtgcga gtggaagaag gaccgtgtat acccatagca aacaaatcga ccttaccgtc
     1801 ccgaataacc aaccgggaaa atgaatccaa gaccgtctga attaccttct gctcc
//
LOCUS       DATALY010000085         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_795974,
            whole genome shotgun sequence.
ACCESSION   DATALY010000085 DATALY010000000
VERSION     DATALY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795974"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..749
                     /locus_tag="VHR42_02545"
     CDS             195..749
                     /locus_tag="VHR42_02545"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX3026094.1"
                     /translation="MNRCGWCESDDLYRKYHDEEWGTPLHDDRKLFEFLVLESAQAGL
                     SWLTVLKKRENYRRAYDDFDFEKVARYDEKKIDSLLQDPGIIRNRRKVESSINNAQRF
                     LEIRREFESFDRYIWGFVGGRPCVGHYREISELPVSTELSGRISRNLKQRGFRFLGPV
                     IVYSFLQAVGIVDDHIESCFRKSR"
     gene            827..1381
                     /locus_tag="VHR42_02550"
     CDS             827..1381
                     /locus_tag="VHR42_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3026095.1"
                     /translation="MKKVIGFSASPRKGNTDTLIKQALIGAETAGAKTKFYKINDLNI
                     KGCQSCFYCRSHDVCAVKDDMQEIYEDLRDADAVVIGSPIYFFQAAGQLKIFLDRLYP
                     MVGDDKGDFRYGQKKGVLIFSQGSSKPANYQTGIDMTKTVVEYMGIDVADTIVFTKAD
                     GVTDASNNEEALQKALEAGKKLVG"
     gene            complement(1468..>2652)
                     /locus_tag="VHR42_02555"
     CDS             complement(1468..>2652)
                     /locus_tag="VHR42_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3026096.1"
                     /translation="GIVAEYNPFHSGHEYQIQTIRESGCTHIVAVMSGNFTQRGEPAC
                     IRKEARCRAALQCGVDLVLELPLPWSTASAETFASGAVTLLDALGCVDLLSFGSECGD
                     ILALTVCAKAALAVTGQPEFKDLLKKGLSFPKARELAMRQLTDIDLSPLSSPNDTLAV
                     EYIKAIIQTGSKMAPMAVRRAAVGHDSEIPGGRFASASLIRKLLQTGKNSEGLNYVPQ
                     PAREIYLRELESRHAPIDLRRAGSLVLSQLRRMSPADFSALPDVSEGLENRIFQSVSR
                     AASLEELYSLIKTKRYTHSRIRRVILSAFLGINRSHTSRPVPYIRVLGFSRAGTGLLR
                     QIKTSAALPIVMRHADIAALDPFAQNIYRLECQATDQYSLCLPCPQPCGMDQRLSAIR
                     NF"
BASE COUNT          677 a          607 c          748 g          620 t
ORIGIN      
        1 tcggcgacaa tgccgtgttt gacgttgacg gcgccgcaaa ctgcggaatt ctcccggtgt
       61 ggtataaagg agcggtgaac caatccaatc gctatgttcc caaaaccaag tgccttgaaa
      121 tcgacgactg ggacgattta aagaaaatgc tgtcagagta aagaaagtac ggaaccggca
      181 ttggggtgaa agaaatgaat cggtgcggct ggtgcgaatc ggatgattta taccgaaaat
      241 atcacgacga agagtgggga acccctctgc atgatgatcg gaaactgttt gagtttctgg
      301 tattggaatc agcgcaggcg gggctcagct ggctgacagt attgaagaaa cgggaaaact
      361 accggcgggc atacgatgat tttgattttg aaaaggtggc gcggtatgat gagaaaaaaa
      421 ttgacagcct gcttcaagat ccggggatca ttcgaaaccg gcgaaaggtc gaatcgtcca
      481 tcaataatgc gcaaagattt ttagaaatcc gccgtgaatt tgaaagcttt gaccgctata
      541 tctggggatt tgtaggtggc agaccctgtg tcggccatta tcgggaaatt tcagagcttc
      601 cggtgtcaac cgaactttcg ggtcggatca gccgaaatct taaacaaagg ggattccgct
      661 ttctggggcc tgtgatcgtc tactcttttc ttcaggcagt cggaattgtc gacgatcata
      721 ttgaatcctg ttttcgaaaa tcccggtaat tccggtgagt tcaatcggct gcggtcttaa
      781 ttgtggagtg cttaatataa ttttaataca aaggagatat cgtattatga agaaagtgat
      841 agggttttca gcaagcccga gaaaaggaaa taccgatacg ctgatcaaac aggcgctgat
      901 cggagcggaa acggcggggg ccaaaaccaa gttttacaag attaacgatt tgaatatcaa
      961 aggctgccag agctgctttt attgccgcag ccatgatgtc tgcgcggtca aagacgacat
     1021 gcaggaaatt tatgaggatc tcagagacgc ggatgccgtt gttatcggct caccgattta
     1081 tttctttcag gcggcgggcc agttaaagat tttccttgac cggctttatc caatggtagg
     1141 tgatgataaa ggcgattttc gctacggcca gaagaagggc gttctgatct tttcacaggg
     1201 ctcatcaaag ccggccaact atcagacagg catcgacatg acaaaaacgg tggtggaata
     1261 tatgggaatt gacgtggcag acaccattgt tttcacaaaa gccgatggag taacggatgc
     1321 ttcgaataat gaagaggctc ttcaaaaagc gcttgaagca gggaaaaaac ttgtcggctg
     1381 agtggttgaa tcatgttttt taaaagaaag caggctcgcg atttatttcg caaagcctgc
     1441 tttctttttt atctgtcttt cggccgatta aaaatttcga atggcgctca gccgctgatc
     1501 catgccgcag ggctgggggc aggggaggca aagagaatac tggtcggtcg cctgacattc
     1561 cagccggtag atgttctgcg caaacgggtc gagagcggcg atgtctgcat ggcgcatcac
     1621 gatggggagc gctgcgctcg ttttgatctg ccgcagaagc ccggttcccg cccggctgaa
     1681 acccagcacc ctgatgtaag gaacaggacg ggaagtatgg gaacggttga tccccaaaaa
     1741 agccgaaagg ataacgcggc gtattctcga gtgggtatag cgcttggttt tgattagtga
     1801 gtaaagctct tccagactgg cggcgcggct tacggattgg aagatgcggt tctcaaggcc
     1861 ctcgctgacg tcaggaaggg ctgaaaaatc cgcgggactc attctccgca attgggaaag
     1921 aaccaggctc ccggctctcc tgaggtctat gggagcatgc ctggattcca gctcccgaag
     1981 atagatctcc cgggcaggct gaggcacata attaagtcct tcggaattct ttccggtctg
     2041 caacagtttc cggatcagag aagcgctggc aaaacggccg ccgggtatct ccgaatcatg
     2101 accgaccgcc gcccgtctga cggccatagg cgccattttt gagccggttt gaatgattgc
     2161 cttgatatat tccacagcaa gagtgtcgtt cgggctgcta agaggtgaaa ggtcaatatc
     2221 cgtcagctgt ctcatggcga gctcccgcgc tttgggaaaa ctgaggccct ttttcagcag
     2281 gtctttgaat tcgggctgcc ccgtaacggc cagcgccgcc ttggcgcaaa cggttagcgc
     2341 caggatgtcg ccgcattcgc ttccgaaact caggagatcg acacagccca gagcgtcgag
     2401 aagtgtgact gcgccggatg cgaaagtttc cgcggacgct gtggaccagg gaagaggaag
     2461 ctccagaaca agatccacgc cgcattgcag cgccgcacgg cagcgcgctt ccttacgaat
     2521 gcaggccggt tcgcctcttt gagtaaaatt gccgctcata accgccacga tatgggtaca
     2581 gccgctttcc cgaatcgttt gaatctgata ttcatgtccg gagtgaaaag gattgtattc
     2641 ggcgacaatt cc
//
LOCUS       DATALY010000086        14338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_106621,
            whole genome shotgun sequence.
ACCESSION   DATALY010000086 DATALY010000000
VERSION     DATALY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106621"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..1603
                     /gene="iadA"
                     /locus_tag="VHR42_02560"
     CDS             488..1603
                     /gene="iadA"
                     /locus_tag="VHR42_02560"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEX3026097.1"
                     /translation="MLTLLNRIECYAPEYIGTNDILIANEKIEKIMPPDSQNWPKEVN
                     TIPCDGLLAFPGLIDQHVHIIGGGGEQGVSSRIAEINSSEIFMAGITTLVGVLGLDRY
                     TKSLESLLAKTKALESEGLTALMYTGSYSVPAVTLTGSIARDLVLIDAIIGVGEIAIS
                     DQRSSQPEFRDLAQLASQAFTGGMVGGKAGIVHIHVGDGKSGLGPLTELLSRSDFSRD
                     QFIPTHVNRNHPLFCQAVQYCRNGGQIDLTAGETAGISVSDAVCRLLAELGSLSGVTV
                     SSDANGSIPGGGVGKIGILFEDLRECIVGRGLSPETILPLVTRNVAKVLKLYPRKGVI
                     REGSDADILITSRDFRVRKLFCKGILKVDQGRPFTDG"
     gene            1616..2431
                     /locus_tag="VHR42_02565"
     CDS             1616..2431
                     /locus_tag="VHR42_02565"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX3026098.1"
                     /translation="MMQDSKGYLIIIGGAEDKQGETEILDQARQMLGEDDLLTVLTTA
                     TESPQEAGKTYQTVFEKIGIKKIEILNINTRDDANNSGMCQVIQHSKCIFFTGGDQLR
                     ITSILGGTRAAVELRTLYQNGGIIMGTSAGASVMSSTMVVQGNDNEPARKCTLKMAPG
                     LGLLTGVIIDQHFDQRGRFGRLLCGVAENPDVLGIGIDEDTAIKVFPDLHFEVIGSNA
                     VTIIDGSTIGSSNVSELKPNEILSINGVTVHTLPGGYGYDMASRKVLRLKHGG"
     gene            2435..5044
                     /gene="cphA"
                     /locus_tag="VHR42_02570"
     CDS             2435..5044
                     /gene="cphA"
                     /locus_tag="VHR42_02570"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX3026099.1"
                     /translation="MDICDLTIFEGRNIYSHRPVMKMVVDIGEYADTPTKDIRGFNEK
                     LLQSFPGLKTNCCGLGYEGGFLEKLRSGTYLAHVLEHVVLEMQFMLGYDVRYGKTRLL
                     EEPSYYYLVYEYENEICGLECGKAATFILNCFLNGEDIEIGEFLDYLKSVSAGADLGP
                     STSAIVKEAKKRGLPITRIGHDSLVRLGYGKHSRLVESTLTDATPCIAADISSNKQLT
                     KSILSEHQIPVPYGKVVYSELSAVMAAGQVGPPVVIKPFDANQGKGVRLNLKTEEEIR
                     IAFQEASKYSSGVIVEKYIQGKDYRILVVGGKVRAVAERLAASVTGDGTHTIGELVEM
                     VNLDPNRGESHEKPLTKIHLNQAERMVLKKKGLTLDSIPGRGDEITLRENANLSTGGI
                     AIDRTDVIHPDNADIAVRAANAIGLDIAGIDVVAGDISHSIVKTGGAVVEVNTAPGIR
                     MHLYPSQGTPRNVASDIVDMLFPTAESKTFPIVSVTGTNGKTTTVRLIAHTLSLSGKT
                     VGMTSTSGTFINGKCICKGDNSGPLSAKCLLSNKQIDAAVLETARGGIVRQGLGYDLA
                     DVGIITNITDDHLGLDGINTLEDLAYVKALVAEAVKPGGSAVLNAADPMTLKILGRVK
                     ADKILFCLNLPEKELFSLKNTLFVYFDSGWITIKSGERLIRVAQIKDIPITSGGIVTC
                     NIENCLAAVSALYALKIPVSTIAEGLKTFENNTGRFDLFNLGNYQVLIDYAHNKAGFE
                     VVFDACSKLKYKRLVGVIGMPGDRNNSSLREVGKLCAQHYDRIYIKEDLDLRGRQPGE
                     VAGVFNDAILSAGYPKDRVSIILKETDALEQAMKTAQSGDLIVVHYEKLQPLVDLVGR
                     AKNNQEKTGALAI"
     gene            complement(5075..6883)
                     /gene="htpG"
                     /locus_tag="VHR42_02575"
     CDS             complement(5075..6883)
                     /gene="htpG"
                     /locus_tag="VHR42_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX3026100.1"
                     /translation="MREGKIQIDSENFMPIIKKWLYSDKDIFLRELVANGCDAIKKYE
                     KLIMMGEAEKDETRPRIRIFVDRENRTIRIEDNGLGMTGEEVEKYITQVALSGAQDFV
                     DRYKDKAGADSGIIGHFGLGFYSAFMVSESVEIQTLSYQKNAEPVYWVSGGDQEYQIG
                     EGSRTARGTTVTLHVAQDESEFLEESRIREILRKYCAFMPYEIFFNPGDDDKDAPVNN
                     PSPLWLKAPKDCTKEEYEKFYSELFMDFNPPLFWIHLNIDYPFNLKGILYFPKQNDKL
                     QVMPGEIKLYSNQVYIADNIKEVVPEFLMLLKGVIDCPDLPLNVSRSFLQNDGDVQKI
                     SRHISKKVSDKLHEIFNDQREQFNNYWEDIAPFIKFGCIKDEGFYDRVKDILLLRTIN
                     DEYLTLQEYPKDQDGRIFYVSDPDMQSQYVRMFRQQGMNAVLLTHMIDSHFISFIEYK
                     EKDFKFARIDSEISSAMKSEEKTEDEQKIAEKFKNSIGKSDLTVSVERLKDAETPAVM
                     LLNEYSRRMQDFSKAYGETFKGANPETTVVLNLENEIVQAIPNLTGEKAETVCRQIYD
                     LAQLSHKSLSAEEMAGFISRSVKLLGELAKPEQENG"
     gene            7114..7827
                     /locus_tag="VHR42_02580"
     CDS             7114..7827
                     /locus_tag="VHR42_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3026101.1"
                     /translation="MTAQLNAHSSVPLYRQLIDSIKHDITTGELKPGDQIPSEIELSE
                     EYQVSRLTVRNAVSILAEEGLVEKVQGKGTYVHSQPQRSLDYALGTTKIALNRGIRLS
                     TKTLQAGYTRANQEQADFFNISPESKLVMIRRVRYLNGYPSILETLYFTPEFELLMTE
                     DLNQSLYSLLGDKYHIVPGKGHKCFEICFADRDESENLCVPAGTPLILCSDGILDTTG
                     NPLHTAKTVYRTDRFKVYI"
     gene            8022..8570
                     /locus_tag="VHR42_02585"
     CDS             8022..8570
                     /locus_tag="VHR42_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX3026102.1"
                     /translation="MKTRNMILAALFAALTAAGSFIRIPIGPVPITLQNLFTLLSGIL
                     LGPGLGALSQALYVLTGLFGLPVFASGAAGFTALVSPSFGYLLGFIAASALTGAICRR
                     GKNSFIRIIAASCAGILAVYALGIPYLYLIFNYVMHAHVTFGTALKTGFLVFLPGDAL
                     KCIAAAVIGSKIGPALRRYRKD"
     gene            complement(8724..9431)
                     /locus_tag="VHR42_02590"
     CDS             complement(8724..9431)
                     /locus_tag="VHR42_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX3026103.1"
                     /translation="MPKRFLFMNMEKESSAKEDSAGEIIRQQKMALESAVSLENDPPS
                     GMEQGFPRINSESEEPKQLYDLSLISNNVVIAGSISSSTGIEINGTVNGNVTCEEELV
                     ISGEINGDIAAKNLDLSGTVNGNVVCDKNIVLSRGANVIGNLEGERITCDGRVSGSMN
                     VKEKVMILSNAEVSGDILSRLISVEEGAIVNGNLKITGNQPREKQEAAESPAKAETDQ
                     TGWETEAGMQNDVGVTC"
     gene            complement(9612..11714)
                     /locus_tag="VHR42_02595"
     CDS             complement(9612..11714)
                     /locus_tag="VHR42_02595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX3026104.1"
                     /translation="MEFYDLLTEKYKIDLNDGQREAVSHVGGPALVLAGPGSGKTTVI
                     TARAARLCLKEGISPRNILTLTFNRAAAQDMKSRFEQVYGESVGGQVRFSTIHGFCNQ
                     ILHDYERRQGHRFKLIESDSQMVRKEHILRGIYQDVNHKPASEEELEALAGEIGLLKN
                     RMIKDVETFESETSTKNFGRIYRDYENLKREKLYLDYDDMLAYAYAVLRKCPDILVRY
                     QERYPFVQVDEGQDLSKIQFELLDLLQTGTSRNLFVVADDDQSIYAFRGAEPDYILNM
                     KQRYEGCQIFRLETNYRSSKNIVETSSRFIGKNLRRFQKKHSTPNGDGVDPSIVRVGN
                     ESEQVDFLINHIERIRTEHPDASVAVIYRNNLSSMALVDALDRRQMYFQLRQNRLHFF
                     HHWVVQDVTALLRFAVDPMDAEAFERFYYKIGRYLSKAMIEFAGHTAAKGSFLNTLLK
                     FPGLQPFQIRNLNELKSDFQRLSGLQPKDAMDEIESVFCYGDFIRDYCEKTGNSSIFA
                     YNIFGILKLIAEGCETVPDFLHRITELRLILQNAAVSAEQSNLTLTTMHSAKGLEFDC
                     VFLIDLTVDEIPGFELKGDENSREAEEERRLFYVGMTRARKLLYLVYPRFRGGVPQER
                     SDFLNEVAACLTCPVKLPAGEIGEGATVVHSKLGTGVVESVEIQGKSVFLNVSFSGTM
                     RTLDYTICKQNGLLEIVE"
     gene            complement(11867..11943)
                     /locus_tag="VHR42_02600"
     tRNA            complement(11867..11943)
                     /locus_tag="VHR42_02600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11907..11909),aa:Pro,seq:cgg)
     gene            complement(12079..12507)
                     /locus_tag="VHR42_02605"
     CDS             complement(12079..12507)
                     /locus_tag="VHR42_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026105.1"
                     /translation="MAGNMPESENDFMQMQQEAIRRVRQMQERARRTLESAGMHIDET
                     GGESQDGPPAAPPIENPAVGPAPRRVPSPSPGHQRIPLPLSGQSGKQPPGIPDDKISI
                     PILNIAVDSDQMLLVLLMYLLIKDGADQWLILSLAYVLLT"
     gene            complement(12520..12888)
                     /locus_tag="VHR42_02610"
     CDS             complement(12520..12888)
                     /locus_tag="VHR42_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026106.1"
                     /translation="MDDITSKLGDLFSSPEGMEKVKNLMSMLTGPGGPMAQQEQQGEP
                     EEENHAPAGGMSDLPFDPMLLMKMKSAMELMNKGDPRVDLLQALKPNLSQDRRKKIDE
                     AIHLMRLINLMPLLKDQGFL"
     gene            complement(12894..13160)
                     /locus_tag="VHR42_02615"
     CDS             complement(12894..13160)
                     /locus_tag="VHR42_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026107.1"
                     /translation="MPNNDFNGKLSQDKLNAMLKMAGQKLGMSPQQLQSVLSDRKQSE
                     QLISKLCGQDDSQIKSAAKDPKSLEEYLKKNPRAKKMLEDLLGG"
     gene            13358..13840
                     /locus_tag="VHR42_02620"
     CDS             13358..13840
                     /locus_tag="VHR42_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3026108.1"
                     /translation="MKNLRIIVVSDTHGDYFNFEKVIETQSKADLFIHLGDGEREADE
                     IADLYPSKKFLTVSGNCDFGSPSPASGEIILAGKRIFYTHGHPYQVKYGLTGLIMEAR
                     NRKADIALYGHTHESYTSYEDGLYIMNPGSLGHPHDGKPTYGVIDLTAAGIVLNIVGV
                     "
     gene            13842..>14338
                     /locus_tag="VHR42_02625"
     CDS             13842..>14338
                     /locus_tag="VHR42_02625"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX3026109.1"
                     /translation="MPNEALFRSFLNTERIGREIFCPETTSSTMDFLKQRADDLSDGA
                     AVICGEQTSGRGRRGRKWTSGKNQSLALSFLLKTPRTRQISAVTLVCGLACANALNRL
                     FGSGFGVKWPNDPIFSEKKIGGILCESILSGDSCSIVCGIGINLTQDAQFFIDAGLPH
                     AASILS"
BASE COUNT         3519 a         3759 c         3388 g         3672 t
ORIGIN      
        1 aacagggaat ttttgagaat tcctattgat tcgggtgttt cttccgccgt taatatctta
       61 ttttgatagg aagacggaat acacccattt ttatgtaaca gagtacattt ttgcaccgca
      121 gttttaggat tcttcccatc gtaacatttt caattcaatt ggcttaaatg ctataatttc
      181 cgtctttttt gccaataatg aatccgtcgg cataaatacg tgatccggtt ctcctgaaca
      241 aactgtttcg gttttgaaaa gcataatgaa agcgattgga ttcatactat tactgccgcg
      301 acaggttatg gctttcattc ggacatccca aattgtcctt acagggatgc accttcctgc
      361 cgcgaatcgg aacagtcgaa ttttgggccg catgtttctt ttgctccgca aaaccgctcc
      421 taaccatttt ggataaaagc ggttttcaga cagacaggat tttttatttt tcaaacggga
      481 tgatgtattg ctgactttat taaataggat cgaatgttat gcaccggaat atatcggcac
      541 aaatgatatt ctgattgcaa acgaaaaaat agaaaaaatt atgccgccgg attctcaaaa
      601 ctggccgaaa gaggtaaaca cgataccatg cgacggcctg cttgcattcc cggggctaat
      661 cgaccagcat gttcacatta ttggcggagg cggcgaacag ggtgtgtcga gccggatcgc
      721 tgaaattaat tcaagtgaaa tatttatggc cgggatcaca accctcgtag gcgttttggg
      781 actcgaccgc tatacgaaaa gtctggaatc gcttctggca aaaacaaagg cgctggaaag
      841 cgagggcctt accgccctga tgtataccgg aagctactcc gttcctgccg tcacgctgac
      901 cggaagcatc gcgcgggatc ttgtcctgat tgatgcgatc atcggagtcg gggaaattgc
      961 catatccgat cagcgttctt cccagccgga attccgcgat ctcgcacagc ttgcatccca
     1021 ggcttttacc ggtggtatgg tcgggggaaa ggccggcatc gtccatatcc atgtcggtga
     1081 cgggaagtcc ggactcggcc ctctgacgga actgctctcc cgatccgatt tttcgaggga
     1141 tcagtttatt cctacccatg taaaccgaaa ccacccgctc ttttgtcagg cggtgcaata
     1201 ctgccgtaac ggcggtcaga tcgatctgac tgccggagag accgcgggta tttccgtatc
     1261 cgacgccgta tgccggctct tggccgaact tggcagcctt tccggtgtta ccgtcagctc
     1321 cgacgccaat ggaagtattc caggcggagg agtgggcaaa atcggcatct tatttgaaga
     1381 tctcagggaa tgtatcgtgg gccggggcct gagccccgaa accatccttc ctctcgttac
     1441 gcggaatgtt gcaaaagtgc tgaaactcta cccacgaaaa ggcgtgatcc gggaaggcag
     1501 cgacgcggat attcttatca ccagccggga ttttcgcgtc cggaaattat tctgcaaagg
     1561 aatcttaaag gtcgatcagg gccggccatt cacggatgga taacaggagg atctattgat
     1621 gcaagacagt aaaggttatc tgattatcat cggtggcgcc gaagacaaac agggcgaaac
     1681 ggaaatcctt gaccaggccc gccagatgct tggcgaagac gaccttttga ccgttctcac
     1741 caccgcgacg gaaagtccgc aggaggcggg caaaacctac cagacggtct ttgaaaaaat
     1801 tgggattaaa aaaattgaaa tattaaatat caacacgcgc gacgacgcaa acaactccgg
     1861 aatgtgccag gtcattcagc attccaaatg cattttcttc acaggaggcg accagctgag
     1921 gattacaagc attctgggcg gaacccgcgc ggcagtcgaa ctccgaaccc tttatcaaaa
     1981 tggcggaatc atcatgggga caagcgccgg ggcctccgtg atgagctcca cgatggtggt
     2041 acagggcaac gacaatgagc ccgccagaaa gtgcacgctg aaaatggctc cggggctcgg
     2101 cctgctgacc ggcgtcatta tcgaccagca ttttgatcag cgcggacgat tcggcaggct
     2161 gctttgcggc gtagcggaaa accccgatgt tctgggaatc ggcattgacg aagataccgc
     2221 aattaaggtc ttccccgatc tccattttga agtgatcggg tcaaacgcgg taacaattat
     2281 tgacggcagc acgatcggca gttccaatgt atccgagctc aagcccaatg aaattttgtc
     2341 aatcaacggc gtcaccgttc atacccttcc cggaggctac ggatatgata tggccagccg
     2401 caaagtttta cgtttaaagc atggaggcta aaaagtggac atttgtgatt tgaccatatt
     2461 cgagggccgt aacatttaca gccacaggcc cgttatgaaa atggtcgtgg atattggaga
     2521 gtatgccgat actccaacga aagacatccg cggatttaac gagaaactgc ttcaatcatt
     2581 ccccgggctg aaaaccaatt gctgtggtct gggatatgaa ggcgggtttc tggaaaagct
     2641 ccgcagcggg acttacctcg cccatgtctt agagcatgta gtactggaaa tgcagtttat
     2701 gctgggttat gacgtacgat acggaaaaac ccggctgctg gaggagcctt cttattatta
     2761 ccttgtttat gaatatgaaa acgagatctg cgggctggaa tgcggaaaag cagcgacgtt
     2821 tattctgaac tgttttctaa acggcgaaga tattgaaatc ggggagtttc tggattatct
     2881 gaaatccgtt tccgcggggg cggatctcgg gcccagcaca tccgcgattg tcaaagaggc
     2941 aaaaaagcgc ggcctgccga tcacccgcat cggccacgac agtcttgtcc ggctcggtta
     3001 tggaaaacac agccgcttgg tcgaatccac tctgaccgac gccaccccct gtatcgcggc
     3061 cgatatctct tccaacaaac agctgacaaa atccattcta agcgaacatc aaatcccagt
     3121 gccctatggc aaagtggttt actccgaact gtcggcggtc atggcggcag ggcaggtcgg
     3181 cccgccggtg gtcatcaagc cttttgacgc aaatcagggc aaaggcgtcc ggcttaatct
     3241 gaaaaccgaa gaggaaatcc ggattgcctt tcaggaggcc agcaaataca gcagcggtgt
     3301 gattgtcgaa aaatatattc agggaaaaga ctaccggatt ctggtcgtcg gcggcaaggt
     3361 aagagcggta gccgaaaggc ttgcagcctc agttaccggc gacggaacgc acaccatcgg
     3421 ggaattggtg gagatggtta atctcgaccc caatcgagga gaaagccatg aaaagccgct
     3481 gaccaagatt cacctgaatc aggcggaacg gatggtctta aagaaaaagg ggttgaccct
     3541 cgactccatt ccgggccgcg gtgatgaaat aacgctccgg gaaaacgcga atctgagcac
     3601 cggcggaatc gccatcgacc gcaccgacgt cattcacccc gacaatgcgg atatcgcggt
     3661 tcgcgccgcg aacgccatcg gactggatat tgccggaatc gatgtggtag cgggagatat
     3721 ttcccattca attgtaaaaa ccggcggagc cgtggtagag gtcaacaccg cgccgggtat
     3781 ccgcatgcat ctttatccca gtcagggtac gccgcggaac gtcgcctcgg atattgtcga
     3841 catgctgttt cccacggcgg aatccaaaac cttccccatc gtttccgtca caggaaccaa
     3901 cggaaaaaca acaaccgtcc ggctcatcgc ccataccctt tcgctgagcg gcaaaaccgt
     3961 tgggatgacc agcacgagcg gaacgttcat caatggcaaa tgcatctgca agggcgataa
     4021 ctccggcccg ctaagcgcca aatgcctgct ttccaacaag cagatcgatg ccgccgtcct
     4081 ggaaaccgca cgagggggaa ttgtccgtca agggctcggt tatgacctcg ccgacgtcgg
     4141 tatcatcacg aatatcaccg acgaccatct ggggcttgac ggcatcaata cgcttgaaga
     4201 tcttgcctat gtcaaagccc ttgtcgccga agcggtcaag cccggcggtt ctgccgttct
     4261 caacgcagcg gaccccatga ctcttaaaat tctgggccgg gtaaaggcgg acaaaatcct
     4321 tttctgtctc aatcttcctg aaaaagagct tttttcactg aaaaacactc tgtttgtcta
     4381 tttcgacagc ggatggatca ccattaaaag cggtgagcgc cttatccgcg tggcacagat
     4441 taaggatatc cccattacat ccggaggaat tgtgacctgc aacattgaaa actgccttgc
     4501 cgccgtttcg gccctttatg cactgaagat tccggtatca acgatagcgg aagggcttaa
     4561 aacctttgaa aataatacag gccgtttcga cctttttaac ctcggcaatt atcaggtatt
     4621 aatcgactat gcccacaaca aggccggttt tgaggtcgtc tttgacgcct gttccaaact
     4681 gaaatacaag cggctggtcg gggtaatcgg catgccgggc gaccggaaca actcctcgct
     4741 ccgggaagtc ggtaagctct gcgcgcagca ttacgaccgc atttatatca aggaagatct
     4801 cgatttaagg ggaaggcagc cgggtgaagt cgcgggtgtg tttaacgatg ccattctttc
     4861 ggccggttat cccaaagacc gggtttccat cattctcaaa gaaaccgacg cgctggagca
     4921 agcaatgaaa acagcgcaaa gcggcgattt gatcgtcgtc cattacgaaa aattacagcc
     4981 gctggtcgat ctggtcggcc gggcaaaaaa caatcaagaa aagaccggag cgcttgcaat
     5041 ttagcgctcc ggcccttttc ttgattttag caaattaccc gttttcctgt tccggttttg
     5101 caagttcgcc cagcaattta acactccggc tgataaaacc ggccatttct tcagcgctca
     5161 gcgatttatg gctcagctga gccagatcgt aaatctgccg gcagaccgtt tcagcctttt
     5221 ccccggtaag attcggaatc gcctgcacaa tttcattttc cagattgagc accaccgttg
     5281 tttcaggatt cgcacctttg aaggtctcgc cataagcttt gctgaaatcc tgcatccggc
     5341 gggaatattc attgagcagc attaccgcag gtgtttcggc atccttaagc cgttcgacac
     5401 ttaccgtcaa atcagatttg ccgatcgagt ttttaaactt ctcggcaatc ttctgctcat
     5461 cttcggtttt ttcttcgctc ttcatggcgc tgctgatttc cgagtcaatc cgggcaaatt
     5521 taaaatcttt ttccttatat tcaataaaac tgataaaatg gctgtcgatc atatgggtca
     5581 gcaacaccgc gttcattccc tgctgccgga acatccgtac atactgtgac tgcatatccg
     5641 ggtcggatac ataaaagatc ctgccgtcct gatccttggg atattcctgt agggtcagat
     5701 attcgtcatt aatcgtgcgc agcagcagaa tgtccttaac ccggtcgtaa aaaccttcgt
     5761 ctttgatgca gccgaatttg ataaagggag caatatcctc ccagtaattg ttgaattgct
     5821 cgcgctggtc attaaaaatt tcatggagct tatccgagac ttttttgctg atatggcggg
     5881 atattttctg aacgtcgccg tcattctgca aaaagctgcg ggagacattc agaggaagat
     5941 ccgggcagtc gatgacccct ttgagcagca tcagaaattc aggaaccact tccttgatat
     6001 tatccgcgat atagacctga ttgctgtaaa gcttgatttc acccggcatc acctgcagct
     6061 tgtcgttctg cttggggaaa tacaatattc ccttgagatt aaacggatag tcgatgttta
     6121 aatgaatcca gaacaacggc ggattgaaat ccataaacag ttcggagtaa aacttttcgt
     6181 attcttcctt cgtgcagtct ttcggcgcct tgagccaaag cggtgacggg ttgttgacgg
     6241 gagcgtcctt atcgtcgtcc cccgggttaa agaaaatttc atagggcatg aacgcgcagt
     6301 atttccgcaa aatctccctg attcttgatt cttcgagaaa ttccgactcg tcctgagcca
     6361 catgcagtgt gaccgttgtg cctcttgcag tccggctgcc ttccccaatc tgatattcct
     6421 ggtcgccgcc ggagacccag tagaccggct cggcgttttt ctgataggaa agtgtctgaa
     6481 tctccacgga ttccgacacc atgaacgccg aataaaatcc gagtccaaag tggccgataa
     6541 ttcccgaatc ggcgcccgcc ttgtctttat aacggtcgac aaaatcctgg gctccggaaa
     6601 gcgccacctg cgtgatgtat ttttccactt cttctccggt cattcccaaa ccgttatctt
     6661 caatgcggat cgtccggttc tcccggtcca caaaaatccg gatgcggggt ctcgtctcat
     6721 ccttttccgc ctcgcccatc atgataagct tctcatattt cttaatggcg tcacagccgt
     6781 tggcgacaag ttccctcaag aaaatatcct tgtcggaata cagccacttt ttgatgatcg
     6841 gcataaaatt ttcggaatca atctgtattt ttccttcccg catttctgtc actccctcta
     6901 atatctactt caagatttaa ccattagtat attttactcg cctttaacaa aaagtcaagc
     6961 gccgctgaaa ccggaagacg ccgaaatggt aaaaggattt gcgctatgtt tcgcaattct
     7021 cgtgatttct aaacgttatt tgtcattata ggtcattata tgatataata accgtatact
     7081 gaacaaatta ataaaggatg cgatgttgac gtcatgaccg ctcagttaaa cgctcacagc
     7141 agtgttcctc tttacagaca gttgattgac tcgattaagc atgatattac gaccggagag
     7201 cttaaaccgg gcgaccagat tccttctgaa attgagctga gtgaagagta tcaggtcagc
     7261 aggcttaccg tccgcaacgc cgtctctatc cttgcagaag aaggccttgt cgaaaaagtg
     7321 caggggaaag ggacctatgt ccacagccag ccccaacggt cgctggacta tgcgctgggt
     7381 acgacaaaaa tcgctctgaa ccgcggcata cggctttcca caaagaccct tcaggccggt
     7441 tacacacggg ccaatcagga acaggcggat ttttttaata tcagcccgga atccaaactg
     7501 gtgatgattc ggcgcgtccg ttatctgaac ggttatcctt ccattctgga aactctctat
     7561 ttcacaccgg aatttgaatt gctgatgacc gaagacttaa accagtctct ttacagcctg
     7621 ttgggcgaca aataccacat tgtgcccgga aaaggacaca aatgctttga aatttgtttt
     7681 gccgaccggg acgaatccga aaatctctgt gttccggcgg gaacacccct gattctctgc
     7741 agcgacggaa ttcttgatac caccgggaac ccgcttcaca ccgcgaaaac cgtctatcga
     7801 acagaccggt ttaaggttta tatttaacag aaagggcccg gcacacttgt gccgggtcct
     7861 ttcttattta attcatatct acttgatcta ttttcaacac agattcgatt cgaatccaat
     7921 aagggaagaa tttcagagga atcggaattg acagccctgc cggtgtggta taaaatgacc
     7981 cattgaaact ttcttatgca atgaaaagga gaatttcgta aatgaaaaca agaaatatga
     8041 tcttggcggc tctttttgcc gcgttgaccg cggccggctc ttttattcga attccgatcg
     8101 gcccggttcc gattacactg cagaatctgt ttactcttct ctcgggaatc ctgctcgggc
     8161 ccggcctggg agccctctca caggcgctgt atgtgctgac cggccttttc ggtcttccgg
     8221 tttttgcatc cggagccgcc ggattcaccg cactggtcag cccgtcattc ggctatcttt
     8281 tgggcttcat tgcagcctcc gcgcttaccg gagcgatctg ccgccgcgga aagaacagct
     8341 ttataagaat catcgccgcg tcctgtgcgg gaatcctggc ggtttatgcg ctggggattc
     8401 cttatctgta tctgattttc aactatgtga tgcacgccca tgtgacattt ggcaccgcgc
     8461 tgaaaaccgg ttttctggtc tttttacccg gggatgccct caaatgcatt gccgccgctg
     8521 tgatcggctc aaaaattggc ccggcgcttc gccgctatcg gaaggactga acttcctgta
     8581 attgcgccgt gcggaaacag gccggattca tcggaataaa gaccggcctt aagacatcgc
     8641 cggatttcag aaaaggatca cggggcacct cccccgtgat ccttttctta ctgccggtta
     8701 gaaaatccgt tttcgatgag aattcagcaa gtgacgccca catcgttctg cataccggcc
     8761 tcggtctccc atccggtttg atccgtctca gccttcgcgg gtgattcggc ggcttcctgt
     8821 ttttcacggg gctggtttcc cgtgatcttc aggtttccgt tcacaatagc cccttcctcc
     8881 acgctgatca gcctggacag aatatcgccc gatacttcgg cattgctgag aatcattact
     8941 ttttctttca cattcatact gccgctgacc ctgccgtcac aggtaattct ctccccttcg
     9001 agattgccga ttacgttggc cccccgggat agaacaatat ttttatcgca cacgacatta
     9061 ccgttgactg ttccggacaa atcaagattt ttggccgcga tatccccgtt gatctccccg
     9121 ctgataacca gctcttcctc gcaggtaaca tttccgttca ccgtaccgtt tatttcgatc
     9181 ccggttgaag aactaatcga gccggcaatc accacattgt tggatataag cgacaaatca
     9241 taaagttgct tcggctcctc ggattcgctg tttatcctgg gaaagccctg ctccatgccg
     9301 gaaggcggat cgttttcaag tgatactgcc gattccagag ccattttctg ctgacggatg
     9361 atttcacccg cgctgtcttc tttcgcgctg ctctcttttt ccatattcat aaacaaaaag
     9421 cgttttggca tactgacaac ctccgtgaat ttgtttacga cggcaatcct tatccgttcg
     9481 ccgcttatcc ggataagtct ggataagcct ataaatattt ccaactgccc tcaatggaat
     9541 catacaacca aataaagtaa atttcaagtc ccgccgcgtc tgcgggaaaa gataggtggg
     9601 aacgatgtaa attactcgac gatttccaga aggccgtttt gtttgcagat ggtataatcc
     9661 agcgttcgca tggtcccgga aaacgaaaca ttgaggaaca ccgattttcc ttggatttcg
     9721 acgctctcca caacgccggt tcccagctta ctgtgaacaa cggttgcgcc ttccccgatt
     9781 tccccggcgg gaagttttac cgggcaggtc aggcacgccg caacctcgtt gagaaaatcc
     9841 gacctttcct gggggacacc gccccggaat cggggataaa caagataaag cagctttcgc
     9901 gcccgggtca tgccaacata gaacagccgc cgttcctctt ccgcctcccg ggaattttca
     9961 tcgcctttca gctcaaatcc gggtatttca tccaccgtca gatcgatcaa aaaaacgcag
    10021 tcaaattcca gaccctttgc ggaatgcatc gtagtaagcg taagattgga ctgttcggca
    10081 gaaactgcgg cgttttgcag gatgagccgg agctccgtaa tcctgtgcaa aaagtccggt
    10141 acggtttcgc acccttctgc gataagctta aggattccaa agatattata ggcgaaaatc
    10201 gaggaattcc ccgttttttc gcagtagtcc cggataaaat cgccataaca aaacaccgac
    10261 tcgatttcat ccatcgcgtc ttttggctgc agcccggaca atctttgaaa atcacttttt
    10321 agctcgttca gatttcgaat ttgaaacggc tgcaaacccg gaaattttaa taatgtattc
    10381 agaaacgacc ctttcgcggc cgtatgaccc gcgaactcga tcattgcttt tgacaggtac
    10441 cgcccgattt tataataaaa tctctcaaaa gcctcggcgt ccatcgggtc aaccgcgaaa
    10501 cgaagcagag ccgttacatc ctgcaccacc caatgatgaa aaaaatgcag cctgttctga
    10561 cgcagctgaa aatacatctg ccgccggtca agcgcgtcaa caagcgccat cgaggataaa
    10621 ttattccgat agattaccgc gaccgatgca tcggggtgtt ccgtccggat gcgttcaata
    10681 tgatttatca aaaagtcaac ctgttccgac tcattcccga ccctgacgat ggagggatca
    10741 acaccgtccc cgttgggcgt cgaatgcttc ttctgaaacc tccgcaggtt tttcccgata
    10801 aagcggctgc tcgtttcaac aatatttttg gaggaacggt aatttgtctc cagccggaaa
    10861 atttgacagc cttcgtatcg ctgtttcata tttaatatgt aatcgggctc tgcgccccgg
    10921 aaggcataaa tgctctggtc gtcatccgcc accacaaaaa ggtttcggga tgtcccggtc
    10981 tgcagcagat caaggagttc gaactgtatt ttggataaat cctgcccttc atccacctga
    11041 acaaacggat accgttcctg atatcggaca agaatatcgg gacacttccg cagaacggca
    11101 taagcgtacg caagcatatc gtcataatca agatataatt tttcccgttt cagattttcg
    11161 taatcacgat aaatccgacc gaaatttttg gtcgacgttt cgctttcaaa cgtttcaacg
    11221 tccttgatca ttcgattttt cagaagcccg atttctcccg cgagcgcttc aagctcttct
    11281 tcactggccg gtttatggtt cacgtcctga taaattccgc gcaggatatg ttccttgcgc
    11341 accatctggc tgtcggactc aatcagctta aaacgatgcc cctggcgcct ttcgtaatcg
    11401 tgcagaattt gattgcagaa accatgaatc gttgaaaacc gtacctggcc tccgacagat
    11461 tccccgtaaa cctgttcaaa ccggctcttc atatcctgcg ccgccgcgcg gttaaacgtc
    11521 agggtcagaa tgtttcttgg gctgattccc tctttcaggc agagacgggc cgcccgcgcg
    11581 gtgataaccg ttgtcttgcc cgaaccgggg ccggccagca ccagggcggg cccgcccaca
    11641 tgggagaccg cctccctctg accgtcgttt aaatcaattt tatatttttc agtcaacaga
    11701 tcatagaatt ccaatacgcc gcaacctttt catttcatca ctttattcga tcataaaaat
    11761 tcgaaacatt ccggcgcttc tttcccgcaa acggcgcctg aaatcacaag aaatgaaaat
    11821 ccctcataaa cggccctatc aaaagccatt tatgagggat tttcattggt cgggatgaca
    11881 tgattcgaac atgcgacctc tgcgtcccga acgcagcgct ctaccaaact gagccacatc
    11941 ccgaattgct tgattattat atcatctcgg ggatacaatg tcaacatcta atcttgtcat
    12001 tcggcgatcg actgacgttt cattcttttt cgcgggcagg caaaaagagg ccgtgtttgc
    12061 cgaaagcaaa cgcggctgtt atgtaagcag aacgtaggca agcgacagaa tcagccactg
    12121 atccgctccg tctttgatca gaaggtacat cagaagcacc agcagcatct ggtcgctgtc
    12181 caccgcgata ttgagaatcg ggatactgat tttgtcatcc ggtattccgg gcggctgttt
    12241 gccggactgc ccggataacg gcaggggaat acgctgatgt cccggagaag gagaaggcac
    12301 tctgcgcggc gccggcccga ctgccggatt ttcgatggga ggggcggcgg gcgggccgtc
    12361 ctgactttca ccgccggtct catctatgtg catacccgcg ctttccagag ttctgcgggc
    12421 gcgttcctgc atttggcgaa cccttcggat tgcctcttgc tgcatctgca tgaaatcatt
    12481 ttccgactca ggcatatttc ccgccatgct ttacacacct cacagaaagc cctgatcttt
    12541 gagcaggggc attaaattga tcagacgcat caaatgaatt gcttcatcaa tttttttcct
    12601 tctgtcctga ctcaggttcg gtttgagggc ctgcagaaga tcgaccctcg ggtcgccttt
    12661 gttcatcagt tccattgcgc ttttcatttt catcagcagc atcggatcaa acggcagatc
    12721 gctcatcccc ccggccggag catgattctc ctcctccggt tcgccctgct gctcctgctg
    12781 cgccatgggg ccccccggac cggtgagcat gctcatcaga tttttcactt tttccatacc
    12841 ctcggggctt gaaaataaat caccgagttt gctggttata tcatccattt ccattatcct
    12901 cccaacagat cctccagcat cttttttgcc cttgggttct ttttcagata ttcttcaagg
    12961 ctcttcggat ctttggcggc ggattttatc tggctgtcat cctgtccgca taatttcgaa
    13021 ataagctgtt cggactgttt ccggtccgaa aggacgctct gaagctgctg aggagacatt
    13081 cccagtttct gacccgccat cttaagcatt gcgttcagtt tgtcctgaga aagctttccg
    13141 ttaaaatcgt tgttcggcat attggctcct cctgttcttc tgttcattgt attctatgcc
    13201 gcgtatgact tgcacattac cctccaatcg tctaaaataa aagaaaacat gaattaatta
    13261 cacttttcaa tttccgcggc tttaaatttg accaaagccg tcgtctttct aacagtatat
    13321 gttttgaagt gtttttcgtg gaaagaatat ctggaaggtg aaaaatttgc ggatcatcgt
    13381 tgtatcggac acacacggag attattttaa ttttgaaaaa gtaattgaaa cacaaagcaa
    13441 agccgatttg tttatccatc ttggcgacgg tgaacgggaa gccgatgaaa ttgcagacct
    13501 ttacccttcg aaaaagtttc tgacggtatc cggcaactgt gatttcggct cgccatcccc
    13561 cgcctcaggt gaaatcattt tagccggcaa gagaattttt tatacccacg gccaccccta
    13621 tcaggtcaaa tacggcctga ccggtctgat aatggaagca cgaaaccgca aagcggacat
    13681 cgccctttac ggccacacgc acgaatccta tacctcctat gaggacggtc tttacattat
    13741 gaatccgggc agtctggggc atccgcacga cggaaagcct acttatggtg tgattgacct
    13801 gaccgcggcg ggaattgttt taaatattgt gggggtctga aatgccaaac gaagcattgt
    13861 ttcggtcgtt tctaaacaca gaacgtatcg ggcgggagat tttctgtccg gaaacaacca
    13921 gctcaaccat ggatttttta aaacaacggg cggatgacct ttccgacggg gctgccgtca
    13981 tctgcggcga gcagacttcc ggccggggca gacgcgggcg caaatggact tcgggtaaaa
    14041 accagtcgct cgctctttca tttttattaa aaacgccccg gacccgtcag atatccgccg
    14101 tcaccctggt ctgcggactt gcctgcgcaa acgcgctcaa ccgccttttt ggaagcggat
    14161 tcggcgtcaa atggccaaat gaccctatct tttccgaaaa aaaaatcggc ggaattctct
    14221 gcgagtcgat tctgtccggc gacagctgtt ccattgtctg cggaatcgga attaatttga
    14281 cgcaggacgc gcagtttttt atagacgccg gtctgccgca cgcggcctct atattgtc
//
LOCUS       DATALY010000087         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_18428,
            whole genome shotgun sequence.
ACCESSION   DATALY010000087 DATALY010000000
VERSION     DATALY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18428"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VHR42_02630"
     CDS             <1..726
                     /locus_tag="VHR42_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX3026110.1"
                     /translation="SPALGEVDKKLGQPHEISLKEILAVLRRYFLMIVSVTIICTVTG
                     FFVTKYVVHPVFEADALLIVNAAAKSDSSGSAASDIALSQSLVDTYAVIMKNDTVLDH
                     VISNLGLSMDSAALYNNIIISQVGTTEVMKLQVKNKDPALALKIAQAIIDYAPGEIVR
                     TVKVGSVKIITPAALAKNPVGPNLSFNTAVAAGVGLGASVIFSLLLDLFNNKFKTEDD
                     VRRMTGMIILGAIPKVNKNNTGS"
     gene            732..1442
                     /locus_tag="VHR42_02635"
     CDS             732..1442
                     /locus_tag="VHR42_02635"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX3026111.1"
                     /translation="MRNAETFSRQEKKRANLILTQTGSFEFIEAFNALRTNLQFASLN
                     RPLHKIVVTSSVIGEGKSTIAVNLAISLAEISYHVLLIDADLRRPNVHKLLGIDNVAG
                     LTTAITEARPWEKCITKIDEDGLSVMTSGPIPPNPVELLVSDRFKQLISALGAAYDFI
                     VIDSPPVSMITDAAVISVVSDGVLFVIRQDYTSFEAVKTAQKNLENVNANLIGCVLNS
                     FKRKDHLMKYGDYYKRDG"
     gene            1486..>2162
                     /locus_tag="VHR42_02640"
     CDS             1486..>2162
                     /locus_tag="VHR42_02640"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX3026112.1"
                     /translation="MIDLHCHILPQLDDGPGSTEEAVKIAQMEAEEGVGMIAATPHFS
                     VIGQDMNQFSRLRKKAIGRLSAAVACITPPVKMIEGAEVALCTQLLDIPDPSFLCYQG
                     TRYMLVEFPPEPDVRLISYMIYQLRLREIIPVIAHIERYGHFIRHPNEINGLIEAGSV
                     IQINASGLLSHGRGMKNNLMRLIDHNMVHLIATDAHSLQNRPPLLRMAMSEVSARLGN
                     DSVELLN"
BASE COUNT          566 a          485 c          557 g          554 t
ORIGIN      
        1 agtccggcgc ttggagaggt ggataaaaaa ttgggccagc cgcacgaaat cagtttaaaa
       61 gaaattttgg ccgtattaag acgatatttt ctgatgattg tatcggtgac gatcatctgc
      121 acagtaaccg gctttttcgt aacgaaatac gtggttcatc cggtttttga agcggatgcg
      181 cttctgattg tcaacgccgc tgctaaaagt gactcgtcgg gcagcgccgc gagcgatatt
      241 gccctgtcac aaagtttggt ggatacctat gccgtcatca tgaaaaacga caccgtactg
      301 gaccatgtga tctcaaatct tggtctttcg atggatagtg ctgctcttta caataatatt
      361 atcatttcac aggttggaac caccgaagtg atgaagctgc aggtcaaaaa caaagatcct
      421 gctctcgcgc tgaaaatcgc gcaggcgatt attgactatg ctccaggaga gattgtccgt
      481 accgtcaagg tagggtcggt aaaaataatt acgcctgccg ctcttgcaaa aaatccggtg
      541 ggcccgaatc tgtcctttaa tacagccgtg gccgccggcg tagggcttgg cgcatcggtg
      601 atattttccc tgctgctcga tcttttcaac aacaaattta aaaccgaaga cgatgtgcgc
      661 agaatgacgg gaatgatcat actcggggcg ataccgaaag tgaataagaa caatacaggg
      721 agctgaagat tttgcgtaac gccgagacgt tttccagaca ggaaaaaaag cgggccaatc
      781 tgattctgac ccaaacgggt tcttttgaat ttatcgaagc gtttaatgcg ttgagaacga
      841 accttcagtt tgcgtctctg aaccgcccgc ttcacaagat tgtggtaaca agttccgtga
      901 tcggagaggg caaatccacg atagccgtaa atcttgccat ttcgctcgcg gaaatttctt
      961 atcatgttct gctgatcgac gccgatttaa gaaggccgaa tgtccataaa ctgttaggca
     1021 ttgataatgt tgcggggctt acaacggcga tcacagaagc acgtccctgg gaaaaatgca
     1081 tcacaaagat tgatgaagac ggcctttctg ttatgacatc ggggccgatt ccgccgaatc
     1141 ctgtcgaact tctggtctca gacaggttca aacagcttat ctccgcgctt ggagcggcat
     1201 atgattttat tgttattgat tctccgcctg tttccatgat caccgacgca gcggtaattt
     1261 cagtagtttc cgacggagtg ctctttgtca tccgtcagga ttataccagt tttgaagcgg
     1321 tgaaaacagc tcaaaaaaat ctggaaaatg ttaacgctaa tctgattggg tgtgtgctga
     1381 attcattcaa aaggaaagat catctcatga aatacggcga ttattataaa agggacgggt
     1441 aaggttttaa agctcttatg atcccttatg aatcggggga tgtttatgat cgatctgcac
     1501 tgccatatcc tgccgcagct cgacgatggg cccgggtcga ccgaagaggc ggttaaaatt
     1561 gcgcagatgg aagcggagga aggcgtcgga atgatcgccg caacgccaca tttttcggtg
     1621 ataggacaag atatgaatca gttcagccgt ttaagaaaga aagcgattgg cagactttcg
     1681 gcagcggtcg cctgcatcac cccgcccgtt aaaatgatcg agggggcgga ggtggcgctg
     1741 tgtacgcagc tgctggatat tcccgatcct tcatttttgt gttatcaggg aacccgctat
     1801 atgcttgtgg agtttcctcc ggaaccggat gtcaggctga tttcctatat gatctatcaa
     1861 ctcaggctgc gggagatcat ccccgtgatc gcgcacatcg agcgttacgg acatttcatc
     1921 cgtcatccga atgaaataaa tgggttgatt gaagccggtt cggtcattca gatcaacgca
     1981 agcggtctgc tgtcgcatgg caggggcatg aaaaataatc tgatgcgatt aattgaccac
     2041 aatatggttc atctgatcgc gaccgacgcc cattcgttgc aaaaccggcc gccgctgctg
     2101 agaatggcca tgagcgaggt atcggccagg ttgggaaacg attctgtcga gctgttaaat
     2161 ca
//
LOCUS       DATALY010000088         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_452219,
            whole genome shotgun sequence.
ACCESSION   DATALY010000088 DATALY010000000
VERSION     DATALY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452219"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1110)
                     /locus_tag="VHR42_02645"
     CDS             complement(154..1110)
                     /locus_tag="VHR42_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX3026113.1"
                     /translation="MAREKTKTITSIGGQALIEGIMMRGPKQTAVSVRLPDQTVSTEY
                     MEIQLLKDKYKWMGWPLIRGVAAFIESLTIGYKALGISADKSGADDEEEELSKFDKWL
                     QKKFGDNLMNVIMTISSILGVLLAIGLFFFLPTWLFNLFRDHIAGEAISPWRSVIEGL
                     MRILLFIGYIVVCSLLPDVRRVFQYHGAEHKTIFCYENKEELTVENVRHHSRFHPRCG
                     TSFMVIMLLLGILIGFLIPFGNPFLRTFFKLLCIPIIVGIGYELIRICGRHDNLATRI
                     ISAPGMWMQRLTTKEPTDDMIEVAIASINAVIPENGEDQIHL"
     gene            complement(1103..2578)
                     /locus_tag="VHR42_02650"
     CDS             complement(1103..2578)
                     /locus_tag="VHR42_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026114.1"
                     /translation="MKIKGITRRWLFNSLGIILLVVIALIATLSFVVQGYVYNGIQQT
                     INGRSGEFINIFYGYGSKTPREFSSAARSYVENSPNKESMEMMVINSVGDIIITSTGF
                     EPDTTQPKPDYDMAYKNTADNYGTWTGNLASGEKVMAVTRVLRNNNGSFMGAVRYVVS
                     LEEADKQVITVVGALMLAGLLVILFVVFSSTYFIRSIVNPIKQIGAIAKRIAQGDFNA
                     RIEKANNDEIGQLCDTINDMAVELGAAEKMKNDFISSVSHELRTPLTAIKGWAETMQM
                     GDNDKDTFDKGMGVIIRESERLSGMVEELLDFSRMQSGRMTLTMDKIDILAELGEAVY
                     MFSERASAEGKFLLYEEPAMLSPVLGDINRLRQVFVNIIDNALKYTTTGGTVNVVVGE
                     SEGFIHVVISDNGCGIPPEHLPNVKKKFYKANQTVPGSGIGLALADEIISMHSGKLDI
                     ESQENVGTAVTIIIPTLKQLEKEPQTDEEITEEKGNNENNG"
     gene            complement(2629..>2780)
                     /locus_tag="VHR42_02655"
     CDS             complement(2629..>2780)
                     /locus_tag="VHR42_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3026115.1"
                     /translation="LKHVWGDAYFGEEKIVDVNIRRLRMKVEDEPSNPKHIVTVWGLG
                     YKWEA"
BASE COUNT          745 a          702 c          582 g          751 t
ORIGIN      
        1 tttcctgcgg cgcagcctcg cccccatgta caataagtgc ctgccggtca agatgaaaca
       61 catgctcaaa caagcacata gcatcaaagg cagggctttc aacccctgcc ttcattaatg
      121 tctgttttcc gtttcgataa acctctgtta aggtcataga tgtatctgat cctctccgtt
      181 ttccggaatt accgcattta tggaggcaat ggcgacttct atcatatcat cggtcggctc
      241 tttggtggta aggcgctgca tccacatgcc gggagcggaa ataatccggg tggcaagatt
      301 gtcgtgtctg ccgcagatgc gaataagctc atagccgata cctacgataa tcggtatgca
      361 taaaagctta aaaaaggtac gtaaaaacgg gtttccgaac ggaatcagaa aaccgatcaa
      421 aatacccagc agcagcataa tcaccataaa gctggtgccg cagcgcggat gaaaccggct
      481 gtggtgacgc acattctcta cggtcagttc ttctttgttt tcatagcaaa aaatcgtttt
      541 atgttccgca ccgtgatact gaaacacgcg ccggacatcc ggaagcaagg aacaaacgac
      601 gatataaccg ataaaaagga ggatacgcat caagccctca atcaccgagc gccaaggact
      661 gattgcttca ccggcgatat gatcgcggaa caggttaaaa agccatgtcg gcagaaaaaa
      721 gaacaaaccg attgctaaaa gaacgcccag aatactgctg attgtcataa taacattcat
      781 taaattgtcg ccgaactttt tttgaagcca cttgtcaaac tttgaaagct cttcttcttc
      841 gtcatccgcg ccggatttat ctgctgaaat accaagcgct ttataaccaa tggtgagaga
      901 ctcaataaaa gcagcaacac cgcggataag cggccagccc atccacttat atttgtcttt
      961 taatagctgt atttccatgt actcggttga aacggtctgg tccggcaagc gtacggaaac
     1021 ggccgtctgc ttggggccgc gcatcataat cccctcaata agcgcctgac caccgataga
     1081 agtaatggtt ttggtttttt ctctagccat tattttcgtt attccccttt tcttctgtta
     1141 tttcttcgtc tgtctgcggt tctttctcca attgttttaa agtaggaata ataatagtaa
     1201 cggcagtacc tacgttttcc tgactttcaa tatccagctt gccggaatgc atggaaatga
     1261 tttcgtctgc aagcgccaag ccgattccgg aaccgggaac tgtttgattc gctttgtaaa
     1321 actttttctt tacgttcggc agatgttcgg gaggaattcc gcagccgttg tcactgatga
     1381 ccacatgaat gaaaccctct gactcgccta ccacaacatt caccgttcct ccggttgtcg
     1441 tatatttcaa agcattatcg ataatattta caaaaacctg acgaaggcgg ttaatatcgc
     1501 ctaatacagg agaaagcata gccggttctt cgtataacag gaatttccct tctgcggaag
     1561 cacgttcact gaacatataa accgcctcac caagctccgc caaaatatcg attttatcca
     1621 tcgttaaggt catgcgcccg ctctgcatgc gtgaaaaatc aagcaattcc tccaccatgc
     1681 cggaaagccg ctccgattcg cggataataa cgcccatgcc tttatcaaat gtgtctttat
     1741 cgttatctcc catctgcatg gtttccgccc aacccttaat ggcggtaagc ggagttcgaa
     1801 gctcatggga aaccgaggaa ataaaatcgt ttttcatttt ttcggctgca ccgagttcca
     1861 ccgccatatc gttgatggta tcacaaagct gcccgatttc atcattgttt gctttctcta
     1921 ttcgcgcatt aaaatcgccc tgcgcaatgc gctttgcgat tgcaccgatt tgttttatgg
     1981 ggttcacgat tgaccggata aaataagtac ttgaaaaaac aacaaacaat atcacaagca
     2041 agccggccag catcaacgcg cccacaacgg tgataacctg cttgtcggct tcctcaagcg
     2101 aaaccacata gcgcacggca cccataaagc tgccattgtt gttgcgcagt acgcgcgtaa
     2161 ctgccattac tttttcgccg gaagctaaat ttcccgtcca ggtaccgtaa ttgtcggcag
     2221 tgtttttata ggccatatcg taatccggct ttggctgagt ggtatccggt tcgaatccgg
     2281 tagaggttat gatgatatct cctaccgagt taatcaccat catttccatg ctttctttgt
     2341 tgggagaatt ctccacataa ctgcgcgcgg cggaactgaa ctcacgcgga gttttactcc
     2401 cgtaaccgta aaaaatattg ataaattcgc cggaacgtcc gttgatggtt tgctggatgc
     2461 cgttatagac atacccctgc accacgaaag agagggtggc aatcagtgca attacgacaa
     2521 gcaatatgat gccgagactg ttaaaaagcc agcgtctggt aatgcctttt atcttcatat
     2581 tgctcacccc ttttcctatt tatgatgcgt taggacaaac agaaattttt acgcttccca
     2641 tttatagcca aggccccaaa ccgtaacaat gtgcttgggg ttggatggct catcttcgac
     2701 tttcattcgc agacggcgaa tgttgacatc cacaattttt tcctcgccaa aatacgcatc
     2761 gccccacacg tgctttaaaa
//
LOCUS       DATALY010000089         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_538432,
            whole genome shotgun sequence.
ACCESSION   DATALY010000089 DATALY010000000
VERSION     DATALY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538432"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..984)
                     /locus_tag="VHR42_02660"
     CDS             complement(112..984)
                     /locus_tag="VHR42_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3026116.1"
                     /translation="MEEIIDEYCWDKDRKLITYEQHKVPGLKNIAHYNLLKAFPPVHT
                     HYHSNIVEFHCLVKGRRIANVEDKNYTITGGECFITFPFEPHSTGNYPLNPCEFYSFQ
                     IDIKEPRLLGLCQELSDYLANQLQHLPHRHLKLGNNDLQNLKASFDCIALGGTDNTYL
                     SLQHLNCFLFNVKNLEPIQEERRPQVNPYIQKSLDYVELHYKDSIPLSTLANIAGYSL
                     SSFKVKFKDAIGITPAEYSTLRKIEYAKKVLLKPDQSITDLAFDLGFSSSNYFCSVFK
                     KYTNIAPSQYCKPE"
     gene            1446..2618
                     /locus_tag="VHR42_02665"
     CDS             1446..2618
                     /locus_tag="VHR42_02665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3026117.1"
                     /translation="MGRVSKLGGSLTRSKIRVMNELCDQYDHVISFAIGEPDFTTPKH
                     IIEAAVNALHEGKTKYSPNAGISKLRQTLAETIKRTHGVDYNWSQEVIITGSGMDTLR
                     LASQAILDPGDEMLVPNPTWSNHPNHPVLFGGKAVMVPVYEKDGFMYDVKVLEKYTTD
                     KTKALLLNSPSNPTGGVISKEQLEALCAFCKDHDLIIISDEVYEHIIYDGLKFYSPAM
                     IEGMKDRVVICNSFSKTYAMTGWRLGYAAGPADIIHAMELINENSISCVNTFVQLAGI
                     EALTAPQDCVADMVSSYQRRRNMIYEGINAIEGLSCIRPKGALYAFINIQKTGLTSEE
                     FAKSLIMKKQVCVVPGSGFGSAGEGFIRICYATSDENIKEGLIRIKEFVDEIMASK"
     gene            2791..3171
                     /locus_tag="VHR42_02670"
     CDS             2791..3171
                     /locus_tag="VHR42_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3026118.1"
                     /translation="MKKFLAILAVLAMTVGITGCSGGASQSQAKPSAGTAATSGFGAD
                     KLEKIKSSGTLVVGCDPTIQNICYLDSSTNSQTGFIPDIVEGFAKQLGVKVKWETLEW
                     SAMISALNRVTIFALGNFARIVSS"
     gene            3235..>3414
                     /locus_tag="VHR42_02675"
     CDS             3235..>3414
                     /locus_tag="VHR42_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026119.1"
                     /translation="MDVSFTHAFAWSSSQQFVSKIWLSRSVVIFMFAATMSTFPLFKA
                     EIIADHSSVSHFTFTP"
BASE COUNT          935 a          742 c          865 g          872 t
ORIGIN      
        1 cattggaaaa agccggaaca gccttcccca aagggttggc tgttccggcc tttttctatg
       61 gcgggcaaaa tatgtgctgg aaaacggcgt cgtttgcgcg ccgattccac tttattctgg
      121 tttgcagtat tgggaaggtg ctatgttcgt atatttcttg aatacgctgc agaaataatt
      181 gctggaggaa aacccaaggt caaatgccag atccgtgatg gattggtccg gctttaataa
      241 aacttttttg gcgtattcga ttttgcgcag ggtactatat tcggcgggtg taatgccgat
      301 cgcgtctttg aacttcactt taaagctgga aagcgaatag cccgcgatat tggccagcgt
      361 gcttaacggt atggagtcct tatagtggag ctcgacataa tccagagact tttgaatata
      421 ggggttaacc tgcggccttc tctcttcctg aatcggttcc agatttttta cgttaaaaag
      481 gaagcagttt aaatgctgca gggagaggta ggtgttgtcg gtgccgccga gtgcgataca
      541 gtcaaaggag gctttcagat tttgaagatc attgttgccc agctttaaat ggcgatgggg
      601 aagatgctgc agctgatttg ccaaataatc gctcagctcc tggcacagcc cgagcagccg
      661 gggctctttg atatcgatct gaaaggaata aaattcgcag ggattcagcg gatagttgcc
      721 ggtgctgtgg ggctcaaagg gaaacgtaat aaaacattcc ccgccggtaa tcgtgtagtt
      781 cttgtcttcc acgtttgcga ttctgcgccc ttttacaagg caatgaaact ccacaatatt
      841 ggaatgataa tgggtgtgca ccggagggaa tgccttcaga agattgtagt gagcaatatt
      901 ctttaatccg ggaaccttgt gctgctcgta agtgatcagt tttctgtctt tatcccaaca
      961 atattcatcg atgatttctt ccataaatct ccttcgaccc caattgtcgt tacgtttcag
     1021 aactcatttt tacatcggag aaaatttcat attggttatc attcttcctg cttgcattgg
     1081 aaaatcggta ggaaaatccg tgtgagtcag agcgattgta tatattctac aaaatgtttt
     1141 aatcgacgtt tgaaaaacta taaaaaaatt taagatatta ttttgaatga acacacacgg
     1201 tctcaggaat ataatcataa catcaatcgg aagcagttca cataaaaccg gctgaaggtt
     1261 aagcatagga agctcagaat accgtactca ggaatcggag aagcctcgtt ctccggagag
     1321 aaacctgatt ctatgataag ggaggaagca caaaaaagca atagcggttg ggaaaagttg
     1381 taaaagtgaa cgtcggcttt caaaatttga aaaggaacga ataagggaag gatttgagat
     1441 tatctatggg acgcgtttct aagttgggcg gttctcttac cagatccaaa atcagagtca
     1501 tgaacgagct gtgcgaccaa tacgatcatg tcatttcgtt tgcgatcggc gaaccggatt
     1561 ttactacgcc gaaacatatt atcgaggctg cggtaaacgc cctgcacgag ggaaaaacca
     1621 agtattcccc caacgcggga atttcaaagc tgaggcagac gctggccgaa accatcaagc
     1681 ggacccacgg cgtcgattat aactggtcgc aggaagtcat catcacgggc agcggaatgg
     1741 ataccctccg actggcaagt caggcgattc tggatcccgg agacgagatg ctcgtcccga
     1801 atccgacatg gtccaaccat ccgaatcatc cggtgctttt cggcggaaag gccgttatgg
     1861 tgccggtcta tgaaaaagac ggcttcatgt atgatgtgaa ggtgttggag aaatatacaa
     1921 ccgacaaaac gaaagccctt ctgttgaatt ccccttccaa tccgacgggc ggcgttattt
     1981 cgaaagaaca gctggaagcg ctgtgcgcct tctgcaaaga ccatgacctg attatcattt
     2041 ccgatgaagt atacgaacat atcatctatg acggactgaa gttttacagc cccgcgatga
     2101 ttgagggaat gaaggaccgc gtcgtgatct gcaattcgtt ctcgaaaacc tatgcgatga
     2161 ccggctggag gctcggctat gcggcggggc ctgccgatat catccacgcg atggaactga
     2221 tcaacgaaaa ctcgatttcc tgtgtgaata cctttgttca gctcgcgggc attgaagcgc
     2281 tgacggcgcc gcaggactgc gtggcggaca tggtctccag ctaccagcgg agaagaaaca
     2341 tgatttacga gggaatcaac gcaatcgaag ggctttcctg catccggccc aaaggcgcgc
     2401 tctatgcgtt tatcaatatt cagaaaaccg ggcttacttc ggaagaattc gcgaagagcc
     2461 tgattatgaa aaagcaggtc tgcgtggtcc ccgggagcgg ctttggatca gccggagagg
     2521 gctttatccg gatttgctac gcaacatcgg atgagaatat caaagaaggc ctcatccgga
     2581 tcaaagagtt tgtggacgag attatggcat caaaataaac gtgagacaag ctgctgaaaa
     2641 caagctgttc attgcgtagt aatacggtaa acactatttc tatggcgaaa tgaaaaaacc
     2701 ggggaatagt gtttttttat caaaaagtcc ttattgtgaa tttaatatac aaatattaaa
     2761 ttataaacat catttttggg aggaaagact atgaaaaagt tcctggcaat tttggcagtt
     2821 ttagcaatga cagttggaat taccggatgc agcggcgggg cttcacagtc acaggcaaag
     2881 ccttctgcgg gcacagcggc cacgagcgga ttcggagcgg acaagctgga aaaaatcaag
     2941 agcagcggga ccttagtggt aggctgcgac cccaccatcc agaatatctg ctatctggac
     3001 agcagcacga acagccagac gggctttatt ccggatattg tcgaaggatt tgcgaaacag
     3061 ctcggcgtaa aagtaaagtg ggagacgctg gaatggtccg cgatgatttc tgccttaaac
     3121 agagtgacaa ttttcgcctt ggggaatttt gcgcggatcg tgtcttcgta agagctgccc
     3181 tgtgtcacgc cgaattttac gtcgggctgg gagatatccg caaaggtctt aaacttggat
     3241 gtatccttca cacatgcttt cgcatggtca agcagccagc aattcgtcag taaaatctgg
     3301 ctttcgcgct cggtcgtcat attcatgttt gccgctacca tgtccacttt tccgctgttt
     3361 aaagcagaaa tcatcgcgga ccattccagc gtctcccact ttacttttac gccc
//
LOCUS       DATALY010000090         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_538442,
            whole genome shotgun sequence.
ACCESSION   DATALY010000090 DATALY010000000
VERSION     DATALY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538442"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..833
                     /locus_tag="VHR42_02680"
     CDS             90..833
                     /locus_tag="VHR42_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEX3026120.1"
                     /translation="MRQSGRNAGVLPGLGGETMSGTLIVVEGLDGSGKTTQMELLEKS
                     FRDSGTACQRVKFPSYDQPFSAPVQMYLNGEFGSKPGDVNAYAASSFFAVDRYASFRK
                     FWHTNYENGETILTDRYTTSNLIYQLPKIPRCEWDTFFSWLCDFEFHKLGIPSPDLTV
                     FLEMPQGISQRLIDSRYQKNGGKRDIHESDTPYLSSCRKSARYAAERLGWHIVSCAEN
                     GTPRSIGDIHNDIVKIIQEAIPGRSHGLF"
     gene            820..1722
                     /locus_tag="VHR42_02685"
     CDS             820..1722
                     /locus_tag="VHR42_02685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3026121.1"
                     /translation="MDSFKDGRMIGFADWNMRRELSILWQECFGDSKRIPDFFLNNIF
                     SPRDCLVYRVGNELAAAVYLIPAGIICGGKTEQAHYIFAAATATRFRSRGFMSSLLAY
                     AAITGAERGDCFSAVLPADDGLYKFYADAGYEDFFQVRIAEADRASLHILASQTGMPG
                     LVLPDFPALNSLRHSCLVRYDGSLMWDDRMFQLSFSMNQVYGDRLVCASQNGGLAYAL
                     CRKENDICEVLESFASRGMYPALVDTLLREVPTQHYRFRLPAGDKRFVNQGQTVRFGM
                     LKALGGSSLTDFALKNPYLGLSMD"
     gene            1768..2304
                     /locus_tag="VHR42_02690"
     CDS             1768..2304
                     /locus_tag="VHR42_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HEX3026122.1"
                     /translation="MIYLFLENGFEEIEALAPIDILRRAGADLKTVGEGGRAVTGAHG
                     VTIMADLEESGVSFGNMEMAILPGGPGTPNHEKSHTVCDALNFCAENDRFIGAICAAP
                     SVLGHMGLLRGHDAVCFPGYEEELGAAHVLRDPVCISRKIITARGAGVAVEFALKLIA
                     VLYGEQKSQEIRKKIQCI"
     gene            2295..2897
                     /locus_tag="VHR42_02695"
     CDS             2295..2897
                     /locus_tag="VHR42_02695"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX3026123.1"
                     /translation="MHLNEIRERKKDLRQQSIHFRGALSNEKKAELDERILDGMLSLE
                     EYSTADVLYTYVSKPAEPDTMTLILAALEAGKRVAVPRCIPETVGMEFYEIHSPKELE
                     PGTYGVLEPVPSRSRLIGMGGKAVCVVPGLSFDLGGYRLGYGKGYYDRFLARFCGVTV
                     GLCYSSCVHQTLPRGFYDRPVDVLITEKHIRRTADAGVLR"
     gene            2914..>3461
                     /locus_tag="VHR42_02700"
     CDS             2914..>3461
                     /locus_tag="VHR42_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX3026124.1"
                     /translation="MKDEQNIDEERRRKVEGFRVQINDDRLTDDTPDTVISSYSDPVE
                     GEKRRERSKTAAADRAQLAHKLRDEEKARKNKSFFRLMWFSFLLMFSLLAAKYLVTGV
                     NDMLAVGRQPVNVTVEIPKNATTSQVTKILYQAGAIRDEQSFSLFTKLTKAPKTYNGG
                     SYQVTTGMDYEALINSIQSRENR"
BASE COUNT          830 a          785 c          957 g          889 t
ORIGIN      
        1 ctcgagctcg taagacctgt ggcgcccgac ctcttttcag cagctgggcc aagcggtgtt
       61 agaggtgctt gcgcggaggg aaggatgtca tgcggcagag cggaagaaat gcgggagttt
      121 taccggggct tgggggcgaa acgatgagcg ggacgctgat cgttgtggaa ggcttggatg
      181 gaagcgggaa aactactcag atggaactgc ttgaaaaatc atttcgggat tcggggacag
      241 cttgccaaag agttaaattc ccttcctatg accagccgtt ttccgcgccc gtgcagatgt
      301 atctgaacgg tgaatttgga tcgaaaccgg gcgatgtcaa cgcctatgcg gcatcgtcgt
      361 tttttgccgt tgaccgctac gctagctttc gaaaattctg gcatacgaat tatgagaatg
      421 gcgaaacgat ccttactgac cgctacacga cttccaatct gatttatcaa cttcctaaaa
      481 tcccacgttg tgagtgggat acgttttttt cttggctatg tgattttgaa ttccataagc
      541 tcggtattcc ttcgccggac ctcacggtct tcctcgaaat gccgcaggga atttcacagc
      601 gcctgattga ctcgcgctat caaaaaaacg gagggaagag ggatattcat gaaagcgata
      661 cgccttacct ttcctcatgt cggaaaagtg cgcgttatgc ggcggaaagg cttggctggc
      721 atatcgtttc ctgtgcagaa aatggaactc cgagaagcat tggggatatt cataatgata
      781 tcgtgaaaat catccaagag gcaattcccg gccggagtca tggactcttt taaggacgga
      841 agaatgattg gatttgcgga ttggaacatg cggcgggaac tttccatcct ctggcaggaa
      901 tgtttcggag attcaaagcg tatcccggat tttttcctaa ataatatttt ttctccgcgt
      961 gactgccttg tttaccgggt gggaaacgag ctcgctgctg cagtctatct tattcctgca
     1021 ggaattattt gtgggggaaa gacagaacag gcgcattaca tctttgctgc ggctacggca
     1081 acccgattcc gttcccgagg atttatgtca tcgcttctgg cctatgcggc aattaccgga
     1141 gcggagcggg gcgattgttt ttctgcagtg cttccggccg atgacggcct ttataagttt
     1201 tatgcagatg ccggatacga ggattttttt caggtccgca ttgcggaagc agacagggca
     1261 tcgttgcata tattggcaag tcagactgga atgccgggtc tcgttttgcc tgacttcccg
     1321 gcgctgaaca gcttgcggca ttcctgcttg gtgcgttatg acggttctct gatgtgggat
     1381 gaccggatgt ttcagctttc tttttccatg aatcaggtct atggggacag gcttgtgtgt
     1441 gcttcgcaaa acggtggact ggcttatgct ctttgtcgta aggaaaatga tatctgcgaa
     1501 gtactggagt cctttgcttc acggggaatg tatcctgcgc ttgttgacac cctgctgcga
     1561 gaagtgccca cgcagcatta ccgttttcgc ctgccggctg gggacaaacg gtttgttaat
     1621 caaggacaaa ccgtccgctt cgggatgctc aaagccctcg gcgggagttc tttgactgat
     1681 ttcgcactga aaaatcctta cctcggtctt tcaatggatt aattattctt atattgataa
     1741 aaatatgcag acaggaggac ttttgcgatg atatatcttt tccttgaaaa cgggttcgaa
     1801 gaaattgaag ctcttgcccc tatcgatatc ctccgccgtg ccggcgcgga cctgaaaacg
     1861 gttggggaag gcgggcgtgc cgtaacgggt gcacacgggg tgacgattat ggctgatctt
     1921 gaggaaagcg gagtttcttt tggaaacatg gaaatggcaa ttttaccggg tgggccggga
     1981 actccgaacc atgaaaaatc gcacacagtt tgcgacgctc ttaacttttg cgcagaaaac
     2041 gacagattta tcggggctat ctgtgcggca ccatccgttc ttgggcatat gggcttgctg
     2101 cgcggtcatg atgccgtctg tttccccggc tacgaagagg agctgggtgc ggcgcatgtc
     2161 ttgcgcgatc ccgtttgtat cagtagaaaa attatcacgg cgcgtggggc aggtgtcgcg
     2221 gtcgaattcg cgctgaagtt aatagcagtt ctatacggag aacagaaatc acaggaaatt
     2281 aggaagaaga tccaatgcat ctgaacgaaa tccgggagag aaaaaaagat ctgcgtcagc
     2341 aaagtatcca ttttcgtggt gctctttcaa atgagaaaaa agcagagctt gacgagagaa
     2401 ttctggacgg aatgctttca cttgaagagt attccactgc tgacgttctt tacacttatg
     2461 tgagcaaacc ggcggaaccg gacacgatga ctttgatcct ggcagcactt gaggctggca
     2521 aacgcgttgc cgtaccgcgc tgcattccgg aaactgttgg gatggaattt tatgaaatcc
     2581 attcgccgaa ggaactcgag cctggtacat acggtgtact ggagccggtt ccaagcagaa
     2641 gtcgacttat cgggatgggc ggaaaggcag tttgtgtagt gccgggtctc agttttgatc
     2701 tagggggata ccgcctcggt tacggaaaag gctactatga ccgattcctc gcaagatttt
     2761 gtggcgttac cgtcggcctt tgctattcca gctgtgttca ccaaaccctt ccgcgcggtt
     2821 tttatgatcg ccccgttgac gttcttatta cggagaaaca tatccgcagg acggccgacg
     2881 ccggggtcct gcgctgatga tcaggaggat ggtatgaaag acgaacagaa tatcgacgag
     2941 gaacgccgga ggaaggttga gggcttccgc gttcagatca atgatgatcg tctgacggac
     3001 gatacacctg acacggtcat cagcagttac agcgaccctg tggaagggga aaagcggcgg
     3061 gaacgttcaa aaacggccgc ggcggaccgc gctcaacttg cgcacaaact tcgtgacgaa
     3121 gaaaaggcgc ggaaaaataa gtcttttttc cgcctgatgt ggttcagctt tttgctgatg
     3181 ttttctcttc ttgctgcaaa atatttggta accggcgtta atgatatgct tgccgtgggc
     3241 aggcaacctg taaacgttac ggttgagatt cccaaaaatg caaccacttc acaggtgaca
     3301 aagattcttt atcaggcggg cgctatccgt gacgagcaat cgtttagcct ttttacaaaa
     3361 ctaaccaaag ctcccaaaac gtataacggc ggcagttatc aggtcaccac cggcatggac
     3421 tacgaagcgc tgattaattc aattcaatcc cgtgagaatc g
//
LOCUS       DATALY010000091         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_19323,
            whole genome shotgun sequence.
ACCESSION   DATALY010000091 DATALY010000000
VERSION     DATALY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19323"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="VHR42_02705"
     CDS             <1..2010
                     /locus_tag="VHR42_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3026125.1"
                     /translation="VKEYTNRLINEKSPYLLQHAHNPVDWYPWGDEAFEKAKAEDKPV
                     FLSIGYSTCHWCHVMERESFEDREVADILNQFFVSIKVDREERPDVDHFYMDACTALN
                     GQGGWPLSCFLTPDRIPFFAGTYFPKKSRYSMPGFMEILTQISDLWKNRRDQITNAGA
                     SLVKESTRNHRSKRKSSPGADSITNAYRQLSMSFDSEYGGFGGAPKFPSLQNILFLLR
                     YGAVYQRDDALKMAERTLRGMAAGGIRDHIGGGFCRYSTDRKWLAPHFEKMTYDNVLH
                     LIAYTEASVVLNPDFAEIAEETAEFLKREMAGPYGGFCTALDADSEGVEGKFYLFAPE
                     DIRTALGDRAEKFCNLYDISGDGNFEGKSIPNLIGRPMPENEKNFAAGCRADLLTFRE
                     KRIPPLKDDKVLAANNGLAAAAFAIAGRVLKKPDYIKLSEQCAGFLLKNLTRSGRLLA
                     RWRDHHADHPSTFDDYAYLVWGLIELYEATFEQSWLELALKWTKKSITLFWDEDGGFY
                     LTGSDVEDLPVRQKSSHDGAIPSGNSVAAMNLIRLARLTGKSSYEQMAVSLIESFGEE
                     IESYPSAFTAMLCARMYLEHGGAEIILTNGEEFDELLKKLPLFSPFTVTAVCGLGHEG
                     LADLAPFMREMKNIDGKAAAYCCKNGACSKPVTDSRAFQKLLEDL"
     gene            2077..2571
                     /locus_tag="VHR42_02710"
     CDS             2077..2571
                     /locus_tag="VHR42_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEX3026126.1"
                     /translation="MKILVDADACPVKNIILKIAKQYGIRVIMLIDTSHRLNDGYSEI
                     VTVDQARDSVDFALIQRTSPGDLVVTQDFGVAAMALAKGARALNQNGLIFDDSNMDGL
                     LYERHISQKIRRSGGRTSNAPRRNRQDDERFESAFRSLLTQAVPVPETGRNNRAKHPQ
                     NNLG"
     gene            complement(2764..3507)
                     /locus_tag="VHR42_02715"
     CDS             complement(2764..3507)
                     /locus_tag="VHR42_02715"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEX3026127.1"
                     /translation="MERIERLAGILSRSERIVAFTGAGVSTESRIPDFRSSGGIYEAI
                     EKEYGYPPEVLLSHSFFTAHPDTFYDYYKKYLIFPEAQPNDAHRSLAALEKSGKLRAV
                     VTQNIDGLHSRAGSQTVYELHGSVCRSHCMKCGKTYEGLDTVLSSSGVPRCGQCGGMV
                     RPDVVLYEEPLDEGVVESAVQAIESADTLLIMGTSLVVYPAAGLIDYFQGKHLILINK
                     SATSYDSRAGLVINAPAGETMKAVMNLIS"
BASE COUNT         1056 a         1044 c          968 g          930 t
ORIGIN      
        1 gttaaagaat atacaaatag acttataaat gaaaaatccc cttatctatt gcagcacgcc
       61 cacaaccctg tggactggta tccgtggggc gacgaggcat tcgaaaaagc caaagccgag
      121 gacaagcccg tgtttttatc catcggctat tccacctgcc actggtgtca tgtcatggaa
      181 agggaatcgt ttgaagaccg ggaagtcgcc gatatcctca atcagttttt tgtcagcatc
      241 aaagtcgacc gggaagagcg gccggatgtt gaccattttt atatggatgc ctgcacggct
      301 ctcaacggtc agggcggatg gccgctttcc tgctttctga cccccgaccg gattccattt
      361 ttcgcgggaa cttattttcc taaaaaaagc cgttattcca tgcccggatt tatggagatt
      421 ttaacccaaa tcagcgacct ttggaaaaac cgccgcgacc agatcacaaa tgccggggct
      481 tcgcttgtta aagaaagcac ccggaatcac cgaagcaagc ggaaatcctc tccgggcgct
      541 gattcgatca ccaacgccta ccggcagctc agtatgtcat ttgattcgga atacggcggt
      601 ttcggcggag cgccgaaatt tccgtcgctc cagaacatcc tgtttctgct gcgctatggc
      661 gctgtctatc agcgggatga cgcgctgaaa atggcggaaa ggacccttag gggaatggcc
      721 gcaggcggta tccgcgacca tatcggcgga ggcttctgcc gttattccac cgaccgcaaa
      781 tggcttgcgc cccattttga aaaaatgacc tatgacaacg tgctccatct aatcgcctat
      841 acagaggcaa gcgtcgtgct gaatcccgat tttgccgaaa ttgcggagga aacagcagag
      901 ttcctcaaac gtgaaatggc agggccttac ggaggattct gcactgcgct tgacgccgac
      961 agcgaaggag tcgaggggaa attctatctg tttgctcccg aagacatccg gacagcgctc
     1021 ggggatcgtg cggaaaaatt ctgcaatcta tacgatattt ccggagacgg caattttgag
     1081 ggcaaaagca tccccaatct gatcggccgt cccatgcccg aaaacgaaaa gaattttgcc
     1141 gcgggatgcc gggccgattt gctcactttc cgtgaaaaaa ggattccgcc gttaaaagac
     1201 gacaaggttc ttgcggccaa caacggcctc gcggccgccg cgtttgccat tgcaggcaga
     1261 gtccttaaaa agcccgacta tattaagcta tcggaacaat gcgccggatt tctcctgaaa
     1321 aatctgaccc ggtcgggacg gcttctggcg cgttggcgtg atcatcacgc cgatcacccc
     1381 tccacgtttg acgattacgc ctatctggtc tggggcctga ttgagctgta cgaggccaca
     1441 tttgagcaaa gttggctcga gttggcccta aaatggacta aaaaaagcat taccctgttt
     1501 tgggatgaag acggcggatt ttacctgacc ggcagtgatg tggaagacct gcccgttcgg
     1561 caaaaaagct cccatgacgg ggcgatccca tccggtaact ccgtcgcggc gatgaacctg
     1621 atccggctgg cgagactgac cggaaaatcc agttatgagc aaatggctgt cagccttatt
     1681 gaaagctttg gcgaagagat tgaatcctat ccgtccgcct tcaccgccat gctgtgcgca
     1741 cggatgtatc tggaacacgg aggggcggaa attatcctca caaacggtga agaattcgat
     1801 gagctgctga aaaagctgcc gttattttcg ccatttacgg tcaccgctgt ctgcgggctg
     1861 gggcatgaag gtcttgccga tctcgcgccg tttatgagag agatgaaaaa cattgatgga
     1921 aaagctgccg catattgctg caaaaacggt gcctgcagca agccggtaac cgactcccgg
     1981 gctttccaaa aacttctgga agatttataa taagattaca gcccggtgga gccttggccc
     2041 atcgcagctg ttaattgata aacaggggtt ttgaaattga aaattctggt cgacgccgac
     2101 gcctgtccgg tgaaaaatat cattctaaaa attgcaaagc aatatggcat ccgcgttatt
     2161 atgctgattg acaccagcca ccgtctcaac gacggttatt cggaaatcgt cacggtggat
     2221 caggcgcggg attcggtcga ttttgcattg atccaaagaa ccagcccggg cgaccttgtc
     2281 gtgacacagg attttggggt tgccgccatg gcgctggcga aaggcgcccg cgcgctcaat
     2341 cagaacggct tgatttttga tgattccaat atggacggtc ttctttatga gcggcatatt
     2401 tctcaaaaaa tcagaaggag cggaggcaga acctccaatg cgccccgccg aaaccggcag
     2461 gacgacgagc gttttgaaag cgctttccgt tcccttttga ctcaggccgt ccctgttcca
     2521 gaaaccggca ggaataaccg tgcaaaacat ccgcaaaata atctcgggtg atgtaatatg
     2581 ccacagaaaa aaagttcagc caaaacacaa gggctcaaag ataccaacaa aaaaccgaag
     2641 ggcgccaata agaaaggcgc acagggtccc aagaaataaa actgtaaaaa agaaaaaggg
     2701 cgcgcgtctg ttcctgattc gggacagcgc ggcccttttg ccgtttttct tgtaagaaac
     2761 aagttatgaa atcaaattca taaccgcttt catcgtttcc cccgccggag cgttaatgac
     2821 aagcccggcg cgggaatcat acgatgtggc acttttatta atcaagatca gatgtttacc
     2881 ttgaaaatag tcaatcaatc ccgccgccgg gtaaacgaca agactggtgc ccataatcag
     2941 aagtgtatcg gcggactcaa ttgcctggac ggcgctttcc accacgcctt catccaacgg
     3001 ctcctcatag agaacgacat cgggccgcac cattccgccg cactgcccgc agcgcggcac
     3061 cccggaggaa gataaaacag tatccagccc ttcataagtc tttccgcatt tcatacaatg
     3121 actgcggcag acgctcccgt gaagctcata aaccgtttgg ctccccgccc ggctgtgaag
     3181 cccgtcaata ttctgggtaa caaccgcccg aagcttaccc gacttttcca gagcggcaag
     3241 tgaccggtgg gcgtcattcg gctgtgcctc cggaaaaatc agatattttt tgtaataatc
     3301 ataaaaggta tcgggatgag cggtaaaaaa gctgtgcgac agcagaacct ccggaggata
     3361 tccgtattct ttttcaattg cttcgtaaat tcctccggaa gaccgaaaat ccggaatcct
     3421 gctctcggta gaaacgcccg ctcccgtaaa tgcaacaatt cgttccgacc gggaaagaat
     3481 cccggcaagt ctctcgattc gctccatatc cgttcctcct ttttttaaaa taactatcgt
     3541 ccttattata gcaccaattc atcgaattta cctgctttaa aaagcgtgat ttttgagatc
     3601 tataaaaaat cagctcccgt cttgactgca actttccacc atgctataat aactaataag
     3661 ttgatgaatt gaatatcaat agatagctaa ccgctaagcg gaggctaagc tgcaaatttg
     3721 tggcttggcc tctttttatt ttttgcctcc ggccaggcct gaaccgaaat tcacatttta
     3781 cgccgaaaac actgctcatt ttccttgaag ctgagaattt ccgatcaaaa caaggaaaac
     3841 taagcatgtc ttaagaaacg gaggaatcat ttttgaaaca accaaaccat atcaaaccca
     3901 ttctcatcct gctcctgtca tttttgattt tgacggcgtc aggctgctcc ggcgggtcgt
     3961 caagcccttc cgtctcctca tccggaacgc ccaaaagc
//
LOCUS       DATALY010000092         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_796878,
            whole genome shotgun sequence.
ACCESSION   DATALY010000092 DATALY010000000
VERSION     DATALY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796878"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VHR42_02720"
     CDS             complement(<1..644)
                     /locus_tag="VHR42_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX3026128.1"
                     /translation="MLNKDHFQNIELEVGYPFNIIYNRSPETFPLHWHNYVEIILPFQ
                     ESVQYAINDRIYSPSPRDILLIWSGELHALVHQPQPSRVLMLQFDYSVIADRSDFRDK
                     LYLFHRLHQIKTTENPELAAALAEKLITLKQLFYGSDNFKETKMCIELYQLFVILGNY
                     LLSDSFSPPSEKKAGPNRTPEQMVSVCSYLSSHCTENIPLSAAAEYAGFSKSHFS"
     gene            853..2202
                     /locus_tag="VHR42_02725"
     CDS             853..2202
                     /locus_tag="VHR42_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3026129.1"
                     /translation="MKSNVTDMTSGNPTGLLLKFSLPMLVGNVFQQLYTMIDSMIVGN
                     YIGADALAAVGATASVLYLFYSLCGGMSIGIGIVVSQYLGARDEESVKRSIANSIYVM
                     LAAALLMSLLGFCFARPVLQLLQTPENIIKEAELFLKTNCAGILAISAYDCVSALLRS
                     LGDSITPLLFLTVSIVVNIGLDFLFVLGFQWGVFGTALATIVAQGIAAIGSLIFAFLK
                     NPYFRIPKQNFRPRKQIIGRCICLGLPVALQSSMIAVSCIALQAVVNSFGSVVVAAFT
                     VTSRLEQLVQQPYSSLGSAVSTYTGQNIGAGQIERVRKGYRKSCLIIGIFAVCVLPMA
                     QFCGYFIMGLFVHDAKVISLGTEALKITSWFYFPLGLIFITRGLLNGAGDTVYSFING
                     VIEMAGRIGLAGPLSKIGGIGVWGIWLATGLTWLITGSVSVTRYFQGTWKCKSVVQR"
BASE COUNT          586 a          505 c          633 g          662 t
ORIGIN      
        1 gaaaaatggg acttactgaa tccggcgtac tcggcggcgg cgcttagtgg aatgttttcc
       61 gtgcaatggc tggagagata ggagcagaca gaaaccatct gctcgggggt ccggttggga
      121 ccggctttct tttcggaagg cggcgaaaag gaatcggata aaagatagtt tccgagaatt
      181 acaaaaagct gataaagctc aatgcacatt ttggtttcct tgaaattatc cgagccgtaa
      241 aacagctgtt tcaatgttat cagcttttct gccaatgccg ctgccagttc cggattttcc
      301 gtggtcttta tttggtggag acgatgaaac aaatagagct tatcccgaaa atcgctccgg
      361 tcggcaatga cggaataatc gaactgcagc atcagaacac gggaaggttg aggttgatga
      421 accagggcgt gcagttctcc ggaccagatt aataagatgt cccggggcga gggggagtag
      481 atgcggtcgt tgattgcata ctgtacggat tcctgaaaag gtaggatgat ctcaacgtag
      541 ttatgccaat ggaggggaaa ggtttccgga gaacgattgt aaatgatgtt aaacggatag
      601 ccgacttcga gttcaatgtt ttgaaaatgg tctttgttca gcatgccgga tgcgccccct
      661 cgttgtgata ataagatgag tacgatatgt tccaatgaga aaaccggatt tatcgaatga
      721 ggaatcacgg taggaatcgc aaaaaatgag cagtaagtcg ttgctttatg gttagtgccg
      781 gatgccgtcc cagagtaaaa taaataagca attcaataaa tgcaaattta tcatcgtcct
      841 gatgaggtga aaatgaaaag caatgtaacg gatatgacga gcggaaatcc cacaggcctg
      901 ctgctgaagt tttctctgcc tatgttggtt ggcaatgttt ttcagcagct ttataccatg
      961 attgattcta tgattgtggg aaattatatc ggcgcagatg ccttggccgc ggtcggagct
     1021 accgcttctg ttctgtatct gttttattcc ctgtgcggcg gcatgtcaat tggaatcggg
     1081 attgtggttt cgcaatatct gggcgcccgt gatgaggaaa gtgtaaaacg ctcgattgca
     1141 aattctattt acgtgatgct ggccgcggcg ctgctgatga gccttctagg tttttgcttt
     1201 gcgagaccgg tacttcagtt gcttcaaact ccagaaaata tcatcaagga agccgaactg
     1261 ttcttaaaga cgaactgcgc cggaattctc gcaatctcgg cgtacgactg cgtttcggct
     1321 cttttgcgtt ctctcgggga ttccatcacg cctctgcttt tcttgacggt atcgattgtg
     1381 gtcaatatcg gtctcgattt tcttttcgtt ctggggttcc aatggggcgt attcggcacg
     1441 gctctggcta ccatagtagc tcagggcatt gccgccatcg gaagcttgat ttttgcattt
     1501 ttaaagaatc cctatttcag aataccgaag cagaattttc gcccccgaaa acagattatc
     1561 gggcggtgca tatgcctcgg gttgccagtg gcgctgcaaa gctccatgat tgccgtttcc
     1621 tgcattgcat tgcaagccgt tgtgaattcg ttcggctctg tcgttgtcgc tgcatttact
     1681 gttacctccc gcctggaaca gctggtacag cagccgtata gttcgcttgg atcggctgtt
     1741 tcaacctata cggggcaaaa tatcggcgcg gggcaaatcg aacgagtgcg gaagggatac
     1801 cggaaaagct gcctgatcat tggaatcttc gcagtttgtg tgctcccgat ggcacaattc
     1861 tgcggatatt tcattatggg cctgtttgta cacgacgcaa aggtgatatc gttaggcacg
     1921 gaagccttga aaattacaag ctggttctac ttcccattgg ggttgatttt tattacgcgc
     1981 gggttgctaa acggagcagg ggataccgtc tattccttta tcaacggagt gattgaaatg
     2041 gccggaagga tcggtcttgc cggtcctttg tcgaaaatag ggggcatcgg cgtctggggg
     2101 atatggcttg cgaccggact gacgtggctg atcaccggat ccgtaagcgt cacccgctat
     2161 tttcagggaa catggaaatg taaatccgtt gtccagagat gactgctttc gaatcgctat
     2221 gtcccatacg gaaaacgagc agtttgatac tgtcccgacc gttcctgtcg ggcacttttc
     2281 tttaagaata tcataaataa caggaaaaat ccatgtgcag gtcaactcct gacacatgga
     2341 ttttttaata tatatttaga aatagtcagc tctgacagat gcgtct
//
LOCUS       DATALY010000093         6134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_710905,
            whole genome shotgun sequence.
ACCESSION   DATALY010000093 DATALY010000000
VERSION     DATALY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710905"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHR42_02730"
     CDS             <1..529
                     /locus_tag="VHR42_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485533.1"
                     /note="Required for the synthesis of the thiazole moiety;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEX3026130.1"
                     /translation="TSERAKMKVLELLGTVAACSSPITCFVVPFTRIQEEIRKNCPEE
                     LFTIIMRRIMMEIATRLAESEGCQALVTGESVGQVASQTMEALFCTDAAAGMPVFRPL
                     IGMDKDEIVRIARKIGTFEISIQPYEDCCTVFTPKHPRTKPVLKYVEAAQNAFDFGPL
                     IDEAAAGAERMRIVG"
     gene            554..1840
                     /locus_tag="VHR42_02735"
     CDS             554..1840
                     /locus_tag="VHR42_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX3026131.1"
                     /translation="MIAEILAVGTELLLGDILNTNAQYLSRELAAMGIGLLHQTVVGD
                     NPERLEQAVREALSRSDILITTGGLGPTGDDITREIVSQAIGKKLVLHEESLKRIEEY
                     FKKAGRPMTENNRKQAYLPEGAIVFANDWGTAPGCAVEAGGKIVVMLPGPPREMTPLY
                     DNCVKPYLAGYSNDVIASVSLRVFGMGESAVENKLTDLMQGENPTLAPYAKEGEVLLR
                     VTAKASGRQQALELCRPVEEEIVKRLGDLVYGRDVEGLQQVVVEQLAKTGRKVALAES
                     CTGGLVAKRITEVPGSSQVFDCGIVSYANNIKQNVLGVHTQTLERHGAVSRETAAEMA
                     EGVRRLAGADIGVGVTGIAGPGGGTEQKPVGLVYVALSQNGKCFVKKLQLYYGSDERE
                     HIRYLASSHALDMIRRAAGGLGQPDSEEIPAPADEK"
     gene            2090..3709
                     /locus_tag="VHR42_02740"
     CDS             2090..3709
                     /locus_tag="VHR42_02740"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX3026132.1"
                     /translation="MGVKYIFVTGGVVSGLGKGITAASLGRLLKARGYRVTIQKFDPY
                     INVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFVDENLSVNSNVTTGRIYWNVITK
                     ERRGDFLGGTVQVIPHITNEIKERIYRVGKSGNTDIVITEVGGTVGDIESLPFLEAIR
                     QAAKEVGHSNAMFIHVTLVPYIYGSGELKSKPTQHSVKELRSLGIQPNTIVCRSERPI
                     PEEVREKIGLFCDVRSEDVIQNLTAPVLYEVPLMLEEEGLAESVCYHLGLENRKPDLA
                     DWAQMVERCKMANGKVTISLVGKYIELHDAYLSVAEALCHAGIANGVKVDIKWVSSEN
                     ITPETVDAMLEGSDGIIVPGGFGDRGISGMIESIRYARTKKIPFFGICLGMQMAIVEF
                     ARNILKFEDADSGEFNPNCAYPVIDLMPEQHEVTEKGGTMRLGLYPCKISENTILRNI
                     YNDELIYERHRHRFEFNNQYREDFLKAGIVTAGLSPDGRLVEMIELPGHPWFVGVQFH
                     PEFKSRPNRPHPLFASFVKAAKGLRQSKTTK"
     gene            complement(3775..4596)
                     /locus_tag="VHR42_02745"
     CDS             complement(3775..4596)
                     /locus_tag="VHR42_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026133.1"
                     /translation="MNKKWYGALFVLFSAGLTASVLLRTCLILYAVDPKTGFYVEGST
                     LVSSLNILLIVFTAILLIPFLMKDRQKSSLPAHSSALSTVSFLLAVSMLLDAVLQLGQ
                     VIFSRGGAGVFVSGITEAIASITFFSLGGQYLSGKKTLHWAVSLLPVVWSAFHLITTF
                     MHYTTIANISEYLFDIVRLVFVLLFLYCHARLTGNVKNYKQVNGLFACGLPAALYSGL
                     STLPHYFAYFSGTNKGSLPTANDAVYLILSIYILILLASLVFGKTAKPQPTEKAE"
     gene            complement(4635..6020)
                     /locus_tag="VHR42_02750"
     CDS             complement(4635..6020)
                     /locus_tag="VHR42_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX3026134.1"
                     /translation="MVWFRQAKEYITKIPNGSLEKLCDEFRKYNRIDPELFETYHVKR
                     GLRNEDGTGVMAGLTLICNVHGYLINEGEKMPIDGELIYRGINVKDLVSGCAAENRFG
                     FEEVVWLLLFGHLPSRQHLDIFNSIIAKSRELPPYFSEDVLMKTASKNIMNMMARSVL
                     ALYAYDSTPEDNSLENVLRQSIELIARMPTLMVHSYQVKRRNFDHASMFFHPLEPSHS
                     TAESILATMRPDKSFTHEEAKLLDLCLMLHAEHGGGNNSTFSCRALTSSGTDTYSAIS
                     AAIGSLKGPRHGGANIKVLEMLESIKSGVQNWEDEDEIAAYLEKILRREAGDKSGLIY
                     GMGHAVYTISDPRAIILKQNAKKLAEKTELFPEFKLLESVEKLTPVVFQKVRGESKIM
                     CANVDLYSGMVYKMLGIPPDLYTPLFAVARIAGWCAHRIEELTTSNRIMRPAYKAVPD
                     KQQYKPLNERI"
BASE COUNT         1569 a         1444 c         1694 g         1427 t
ORIGIN      
        1 taccagcgaa agggccaaaa tgaaagttct tgagctgctc ggaacagttg ccgcttgcag
       61 ttcccccatc acctgttttg tcgttccttt tacaaggata caggaggaga tccggaaaaa
      121 ctgccccgaa gagcttttta ccatcataat gcggcgtatc atgatggaga tcgcgacaag
      181 acttgcggag tccgaaggct gtcaggcttt ggtgaccgga gaaagcgtcg gtcaggtggc
      241 cagccagacc atggaggcat tgttctgtac cgacgcggcc gccgggatgc cggtgtttcg
      301 cccgctgatc gggatggaca aggatgagat cgtcaggatc gcgcgaaaga tcggaacctt
      361 tgaaatctcc atccagccct atgaggattg ctgcacagtg tttaccccga aacatccccg
      421 tacaaagccc gtcttaaagt atgttgaggc cgctcaaaat gcctttgatt tcggccctct
      481 gatcgacgag gctgcggcag gggcggaaag aatgagaatt gtgggataaa tttcccgaaa
      541 ggatgaatgg aatatgatcg cagaaatttt ggctgtggga acggaattac ttctcggcga
      601 tattctgaat accaatgcgc agtatctttc ccgcgaactt gccgccatgg gtatcgggct
      661 gctgcatcag acggtggtcg gcgacaaccc cgagcggctt gagcaggctg tccgggaggc
      721 gctgtcgcgc agcgatattc tgattacgac gggagggctt ggcccgacag gcgacgacat
      781 cacccgggaa attgtctcgc aggcaatcgg aaaaaagctg gttcttcatg aggaaagcct
      841 gaagaggatc gaagaatatt tcaaaaaggc cggaaggccg atgacggaga ataaccggaa
      901 acaggcgtat ttaccggaag gagccattgt tttcgcgaac gactggggaa ccgctcccgg
      961 ttgtgcggtg gaggccggag gaaaaatcgt tgtaatgctg ccaggccccc cgcgtgaaat
     1021 gacgccgctt tatgacaact gtgtgaaacc ttatctggcc ggttattcca acgatgtgat
     1081 tgcttcggtg tcgctgcgcg ttttcggtat gggtgagtcg gcggtcgaaa acaaactgac
     1141 cgacctgatg cagggcgaaa atccgaccct tgccccttac gccaaagaag gggaagtcct
     1201 gctgcgcgtt accgccaagg cttccggtag gcagcaggct ctggaacttt gcaggccggt
     1261 cgaggaggag attgtcaagc gccttggcga cctcgtctat ggcagggatg tcgaagggct
     1321 gcagcaggtt gtggtggagc agcttgcaaa aacaggcagg aaagtcgcgc tggccgaatc
     1381 ctgtaccggc gggttagtgg ctaagcgtat caccgaggtc cctggaagtt cgcaggtctt
     1441 tgactgcgga attgtcagtt atgccaacaa tataaagcag aatgttctgg gtgtgcatac
     1501 gcagactctc gaacggcacg gcgcggttag cagagaaacc gcggcagaga tggcggaggg
     1561 agtgcgacgg cttgccggag cggatattgg cgttggtgta accgggattg ccgggccggg
     1621 cgggggaacg gaacaaaaac cggttgggct tgtatatgtc gccctttccc agaacgggaa
     1681 atgttttgtc aaaaagcttc agctttatta tggcagcgac gagcgtgagc atatccgtta
     1741 tcttgcatcg tcccatgcgc ttgatatgat ccgccgcgca gccggtgggc tcggtcagcc
     1801 ggattcggag gagattccgg ctccggcaga cgaaaaatag aaatcatacg gaataccttc
     1861 cgggcccggg tatgccggaa cccggccgcg gagccgattg attcggccga agaaaaggaa
     1921 gggccctttt gtcccgggct gggtcagaaa atgcgacaac aaaagtcgcg cttttccgca
     1981 gaagtcgtca caattataca aataggaatg aaattggtca aatagccttg acctgaactt
     2041 gcaaaaaggt taaaataatg ggtatgtttt ttgagggagg aaaggtgaaa tgggagttaa
     2101 atacatcttt gtgaccggcg gtgtcgtatc cggtcttggc aaaggcataa cggcggcgtc
     2161 tcttggcaga ttgctcaaag caagaggata ccgtgtaacg atacaaaagt ttgatcctta
     2221 tattaatgtg gatccgggaa caatgagccc ttaccagcac ggcgaggttt ttgtaaccga
     2281 cgacggagcg gaaaccgacc tcgaccttgg gcactatgag agatttgtcg acgaaaattt
     2341 gtcggtcaat tccaatgtga cgaccggaag aatttactgg aacgttatta ccaaagaaag
     2401 acgcggcgat tttctgggtg gaacagttca ggtgatcccg catattacaa atgaaataaa
     2461 agagcgcatc tatcgtgtag gaaaaagcgg aaataccgat atcgtgatca ccgaagtcgg
     2521 cggaacggtc ggcgatattg agtcactccc ttttcttgaa gcgattcggc aggctgcaaa
     2581 agaagtcggg cacagcaacg cgatgtttat acatgtcacg cttgtgccct atatttatgg
     2641 gtccggcgag ctgaagtcca aacccacaca gcatagtgtc aaagagctgc gttcactggg
     2701 cattcagccc aacaccatcg tttgccgcag tgagaggcct attccggaag aagtcaggga
     2761 aaaaatcggg ctgttctgcg atgtaagatc tgaggatgtg attcagaacc tcacggctcc
     2821 cgttctttat gaagtgccgc tgatgctgga agaagagggc cttgccgaat cagtttgcta
     2881 tcatctgggg cttgaaaacc gcaaacctga tcttgccgat tgggctcaga tggttgaacg
     2941 ctgcaagatg gctaatggga aggttacaat atctctggta ggcaaatata tcgaactgca
     3001 cgacgcatat ctgtcggtgg cggaggctct ttgccacgcg ggaatcgcca acggcgtcaa
     3061 ggtcgatatc aaatgggtaa gctctgagaa tatcactccg gagacggtgg acgccatgct
     3121 ggagggaagc gacggcatta ttgttccggg aggcttcggt gaccggggaa tctcaggtat
     3181 gatcgaatca atccgttacg cgagaaccaa aaagattccg ttcttcggaa tttgtctcgg
     3241 catgcagatg gcgatagtag aatttgcgag aaatattctg aagtttgaag atgcggattc
     3301 cggcgagttt aatccaaact gtgcctatcc tgtgatcgac ctgatgcccg agcagcatga
     3361 ggtgaccgaa aagggcggaa caatgcggct tggtctttat ccgtgcaaaa tttccgaaaa
     3421 cacgattctg cggaacattt acaatgatga gctgatttat gagaggcaca ggcaccgctt
     3481 tgagtttaac aatcagtatc gtgaggattt tctcaaagca ggaatcgtta ccgccggtct
     3541 ttctccggac ggcaggctgg tggagatgat agagctgccc gggcatccgt ggtttgtcgg
     3601 cgttcaattc catccggaat tcaaatcccg tcccaacaga ccccatccgc tatttgcgtc
     3661 gtttgtcaag gctgcgaaag gcctcagaca aagtaaaaca acgaaataat gatctcaaat
     3721 ggaaaagagc cgggccccgt tcggggcccg gctctaaatt tttttgaaga ctgattactc
     3781 cgctttctcg gttggctgag gtttcgcagt tttcccaaag acaagcgatg cgagcagaat
     3841 caggatgtag atggatagaa tcagataaac cgcgtcgttt gccgtgggca gtgaaccttt
     3901 gttcgttccg gaaaaataag caaagtaatg cgggagggtg gatagcccgc tgtaaagggc
     3961 cgcgggcaaa ccgcaggcaa acaacccgtt cacctgttta taatttttca cattgccggt
     4021 cagtctcgcg tggcagtaga ggaacagcag cacaaaaacc agccttacga tatcaaacag
     4081 gtattccgat atattggcaa tcgtggtgta atgcataaag gttgtgatga gatgaaatgc
     4141 ggaccagact accggaagca gagacacggc ccaatgcaat gtttttttgc cggaaagata
     4201 ttgcccaccg agagaaaaga aagtgatcga agcaattgct tcggtgattc cgctcacaaa
     4261 gactccggcg cctccacggc tgaaaatgac ctgccccagc tgcagcacag cgtccagcag
     4321 catagaaacc gcgagcagga aagaaaccgt gcttaaagcc gaagaatgcg cagggagcga
     4381 agatttctgc ctgtctttca tcaaaaacgg aatcagcagg attgctgtga aaacaatcaa
     4441 taagatattc agtgaagaaa caagagtgct tccttctaca taaaaaccgg ttttagggtc
     4501 aaccgcataa agtatcaggc atgtgcgcaa caaaacagaa gcggtcaggc cagcggaaaa
     4561 aaggacgaat agagctccat accatttctt attcacctgc ggttacctcc aatggtgcca
     4621 agttataaaa ccagtcagat acgctcgttc agcggtttat attgctgctt gtcgggaact
     4681 gccttgtagg ccggacgcat gatccggttg ctggtggtga gttcctcgat tctgtgggcg
     4741 caccagccgg ctatgcgtgc aacggcgaac aggggagtat acagatccgg cggaatccca
     4801 agcattttat agaccatgcc ggaatacaga tcgacgttcg cgcacataat cttactttct
     4861 cccctgacct tctgaaacac gacgggagtg agtttctcca ccgattccaa aagtttgaat
     4921 tcaggaaaga gttcagtttt ttcagcaagc tttttcgcgt tctgctttaa tattatagca
     4981 cgcggatcgg aaatcgtata gaccgcgtgg cccattccgt aaattaaacc ggatttgtcg
     5041 cctgcctcgc ggcggagaat tttttcaaga taagccgcga tttcgtcttc atcctcccag
     5101 ttttgaactc cgcttttgat gctttcaagc atttccagga ccttgatgtt tgcaccgccg
     5161 tgccgcgggc ctttcaggct tccgattgcc gcggaaatgg ccgaatatgt atccgtaccg
     5221 gaagaagtca gcgccctgca ggaaaacgtg gagttgtttc cgcccccgtg ttcggcatgg
     5281 agcatcaggc agagatccag aagtttcgct tcttcgtgcg taaagctttt gtccggcctc
     5341 attgtggcga gaatgctttc cgccgtcgag tggcttggct caagcgggtg gaagaacatg
     5401 ctcgcatggt caaagttacg ccgcttgacc tgataggaat gcaccatcag ggtgggcatg
     5461 cgggcaatca gctcaattga ctggcgcagc acattttcca gggagttgtc ttccggggtg
     5521 ctgtcataag cgtagagcgc cagcaccgag cgggccatca tattcattat attttttgat
     5581 gcggttttca tcaatacgtc ttcggagaaa tagggaggca gctcacggct cttggcaatg
     5641 attgagttga agatgtcaag atgctgccgg cttggaaggt gcccgaacaa aagcagccaa
     5701 acgacttctt caaatccaaa cctgttttcc gcggcgcaac cgcttaccag atccttgaca
     5761 ttgattcctc tgtagatcag ttccccgtca atgggcattt tttcaccttc attaatcagg
     5821 taaccatgca cattgcagat cagggtgaga ccggccatca cgcccgttcc gtcttcattc
     5881 cgaagacctc tcttgacgtg gtaggtttca aacagctccg ggtcgatcct gttatatttg
     5941 cgaaattcat cgcataactt ttcaagggag ccgtttggaa tttttgtaat atactccttt
     6001 gcttgacgaa accataccaa atgagttaga atataaatgt ttacatcctt tttaccatac
     6061 tcattaacaa tcctcagaat cggccgttgc ccgcttcggc ggcggcggaa aattcataaa
     6121 cggaaggagt gcca
//
LOCUS       DATALY010000094         3953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_710948,
            whole genome shotgun sequence.
ACCESSION   DATALY010000094 DATALY010000000
VERSION     DATALY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710948"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1158
                     /gene="spoIID"
                     /locus_tag="VHR42_02755"
     CDS             151..1158
                     /gene="spoIID"
                     /locus_tag="VHR42_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HEX3026135.1"
                     /translation="MKGIRITAAIFIMLMLLVPMISIPINANMQQKTVNYSYKKESST
                     EIAQTFRLLVTSTGKVENISAFDYICGVVAAEMPASFHLEALKAQAVASFTYACYKRE
                     SNLENPAANSVIKGADLTDDSSKFEAYISKTEAQSRWGSNFSQKWGEIEKAVSGVRNK
                     VMVYNGRPIAAVFFSLSSGKTESSKDVWGSDLPYLSEVDSHWDQQVEGFETRVTLSEK
                     EFQSKIKAAYSHTVFPKDPSRWIGTVKRSGAGGVISAVICGNSVKGGTIRQIFGLRSP
                     NFSVSYKNSVFTFVVKGNGHGVGMSQHGADYLASRGENYQQILTYYYKGIQLTDYQWN
                     R"
     gene            1251..2003
                     /locus_tag="VHR42_02760"
     CDS             1251..2003
                     /locus_tag="VHR42_02760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3026136.1"
                     /translation="MSNFKVGNSRFSRFMNGKGFYIALALCLVAVGSAAYIAANKSLG
                     ISQSPQTSSNSAVSNYTPSQDSSLTSSSTQQTGNNVSGVPDDSSSSSTASAATGTSSS
                     SSTGSSAKSAAPTFYVLPVQGSVITKYSPDTPLYDKTMDDWRVHDGVDIASDQGTPVK
                     ACADGTVTAVTKDAILGQVVEITHGGGLKSLYANLSTNVTVKKAQKVEAGQVIGCVGQ
                     TAQGEISLVPHLHFAMTKNGNYVDPLITIGKK"
     gene            complement(2292..2438)
                     /locus_tag="VHR42_02765"
                     /pseudo
     CDS             complement(2292..2438)
                     /locus_tag="VHR42_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
     gene            complement(2425..3948)
                     /locus_tag="VHR42_02770"
     CDS             complement(2425..3948)
                     /locus_tag="VHR42_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3026137.1"
                     /translation="MAYCRVSTNKEEQLDSLASQRKFFAEYCRRNGYRLMKIYADEGK
                     SGTKMKNRVQLLRLLSDAERGEFRLVLIKDVSRLARNTVDFLTSIRKMKFLGIKVVFV
                     NYDQTSSDSSEFMLTMLSAIAQEESANTSRRVKFGKKQNAEKGRVPNIVYGYDKIPGD
                     YFHLRINPIEAGVVRKIFQMYVGQELGAGKIAEVLNKEGFKTKRGCPFTQNAVQRILT
                     NELYIGSVVNARQEVEDFLTGKRRSNNEKDWLRTNRPELRLIDDETFLKAGDIRNSRS
                     RLFHSGFRLSERNLFSKLIRCGSCGASFRRQERTYKNTYIRWVCSGRNAKGAGYCPNK
                     TALEEDTLLAAIRAYLSGFLQKRSSAPREILREFSRQFCQKDESATLEKELESLLVRA
                     KKTRMRYLSMYEAEIITLEELRGKASELDLEISRLTNELGGVREETDTMGLAQTILAE
                     ILENAGEMISNEFLTSSVLHSLIEWIEVDESQRVTVHLKSFHSPQRGKIVPITDDRT"
BASE COUNT          996 a          929 c         1008 g         1020 t
ORIGIN      
        1 tggcactcct tccgtttatg aattttccgc cgccgccgaa gcgggcaacg gccgattctg
       61 aggattgtta atgagtatgg taaaaaggat gtaaacattt atattctaac tcatttggta
      121 tggtttcgtc aagcaaagga ggtgaaaaaa atgaagggca tcagaattac ggctgcaata
      181 tttataatgc tcatgctctt ggttcccatg atttcaattc ctattaatgc aaatatgcaa
      241 caaaaaacag taaattattc atataagaaa gaaagttcga cagaaatcgc gcagactttc
      301 cggcttcttg ttacctccac cggaaaggtt gagaacattt ccgcttttga ttatatctgc
      361 ggagtggtcg ccgccgaaat gcctgcgtct tttcatcttg aggcactaaa ggcccaggcg
      421 gtggcgtcgt ttacctacgc gtgctataaa agagagagca atctggagaa cccagcggcc
      481 aattcggtga tcaaaggcgc tgatttgacg gatgacagtt cgaaatttga ggcgtatatt
      541 tctaaaaccg aagcgcagtc caggtgggga agtaatttca gccaaaaatg gggcgagatt
      601 gagaaagccg tcagcggggt ccgaaataag gtcatggtct acaacggacg cccgatcgcc
      661 gctgtttttt tctctctttc ttccggaaaa accgaaagca gcaaagacgt atggggaagc
      721 gacctgccat atctgagtga ggttgacagc cattgggatc agcaggttga gggctttgaa
      781 accagagtca cgctgagcga aaaggagttt caatcgaaaa taaaagcggc ttacagccat
      841 acggtgtttc cgaaagaccc cagccgctgg atcggcacgg taaaaagatc cggcgcgggc
      901 ggagtaatca gcgcggtgat ttgcggaaac tctgttaagg gcgggacaat ccggcagata
      961 tttgggcttc gttcccccaa tttcagcgtc agttataaaa attcggtttt tacatttgtt
     1021 gtgaaaggaa acggacacgg tgtgggaatg agccagcacg gagcggatta tctcgcttcc
     1081 cggggggaaa actaccagca gattttaacc tattattaca aaggaattca gctgaccgat
     1141 tatcagtgga atcgttaagc tgttttattg tatcaggtct taaataaatt ggaagttatc
     1201 ttgtataaca gtgcaaaatt ccggcaaaaa taaagccgga ggtgcacaag atgagcaatt
     1261 ttaaagtagg taattcccgc ttttccagat ttatgaacgg taagggcttc tacatagccc
     1321 ttgctctttg cctggttgcc gtaggatcgg ccgcgtatat tgcagcaaat aaaagtttgg
     1381 gcatttcaca gagcccgcaa accagcagca attcagcggt ttcgaattat acgcccagtc
     1441 aggactcaag cttgaccagc agcagcaccc agcagaccgg aaacaatgtc agcggagttc
     1501 ccgatgacag cagttccagt tccacagctt ccgccgcgac gggaacatct tcaagcagca
     1561 gcaccggttc ctcagccaaa tccgcagcgc ccacctttta tgttcttccg gtgcagggca
     1621 gtgtcataac caaatacagc ccggataccc cgctgtatga taagaccatg gatgactggc
     1681 gggttcatga cggcgtggat atcgcttccg atcagggaac gccggttaaa gcctgcgccg
     1741 acggcactgt gaccgcggtc acaaaagacg cgattctcgg tcaggtggtt gaaatcacac
     1801 atggcggagg gctcaaatcg ctttacgcca atctttctac caatgtaaca gtcaaaaagg
     1861 ctcaaaaggt cgaggccggt caggtgatcg ggtgtgtcgg acagacagcg cagggagaaa
     1921 tctcgctggt tccgcatctg cattttgcca tgaccaaaaa tggaaattat gttgatccgc
     1981 tgataactat cggcaaaaaa taaaatatga ttcggcggta tctcttttgc gcgccgtatg
     2041 tcggggtcag aaaaccggat acggcgggac aggggtgggt gtcacgcgcg gtttgtatcc
     2101 ggtgttccga aggaaagatt ccggatacgg ggtcccggat gatgggattg aaatacggcg
     2161 tgtgacgaat aaaaagctgc gcggtttgcc gcgcagcttt ttattgttga attttaaaat
     2221 accgggccaa acggagaatc gcgcagaaag ccccaaaacc tggaaacaat tgaaaattga
     2281 tcccgggaac attattcatg gcggtatttt cttcgcgttg cgagaccacc ccggatatgc
     2341 cgctgcgctt tattgatttc taaaatgtgt ttgacttcga cgtaaagctg cggattcagt
     2401 ttttttaagc gctcagaaac gtctttatgt acggtcgtca gtaataggaa cgatttttcc
     2461 ccgttgagga ctgtgaaaag acttaagatg tacggtaacg cgttggcttt catcgacttc
     2521 gatccattca atcaggctgt ggaggaccga gctggtcaga aattcgtttg agatcatctc
     2581 tccggcgttc tctaaaattt cggcgagaat agtctgggca agccccattg tatcggtttc
     2641 ttccctgacg ccgccgagtt catttgtgag ccggctgatt tccaaatcga gttccgaggc
     2701 cttcccacgg agctcctcga gggttataat ctccgcttca tacatcgaga gatacctcat
     2761 tctggttttt tttgctctta ccagaaggga ctccagctct ttttccagag tggcggactc
     2821 gtctttttga cagaattgtc tgctgaattc ccggagaatt tcccgcggag cgctcgatct
     2881 tttttgaaga aatccggaga ggtaagcccg aatggcggcg agcagcgtat cttcttccaa
     2941 cgctgtttta ttcgggcagt aaccggcccc ttttgcgttt cgcccggagc agacccatcg
     3001 gatataagtg tttttatagg tacgttcctg acgacggaaa gacgcgccgc agcttccgca
     3061 tcgtatcagc ttgctgaaaa gattgcgttc gctcaggcga aaacccgagt ggaacagtct
     3121 tgagcgggaa tttcggatgt ctccggcttt taaaaaggtc tcgtcgtcaa tcagccgcaa
     3181 ttccggcctg ttggttctga gccaatcttt ttcattgttg ctgcggcgct ttccggtgag
     3241 aaaatcctca acctcctgcc ttgcgttgac gacgcttccg atgtacaatt cattcgtcag
     3301 aatacgctgg acggcattct gggtgaaagg gcagccgcgt ttggttttga acccttcctt
     3361 gttcagaact tcggcaattt tgcccgcacc caattcctga ccgacataca tttgaaagat
     3421 tttgcgcact actcccgctt cgatcggatt aatccttaaa tggaagtagt cgcccggaat
     3481 cttgtcatag ccgtagacga tattgggaac ccggcctttt tccgcgtttt gtttcttacc
     3541 gaattttaca cggcgggaag tgttggcgct ctcctcctgg gcgatggccg aaagcatggt
     3601 gagcatgaat tccgagctgt cagacgaggt ttgatcataa ttaacaaaaa ccactttgat
     3661 gcccagaaac ttcatttttc ggatgctggt caagaagtcg acggtgtttc ttgcaagacg
     3721 ggaaacgtcc ttgatcagaa caagccggaa ttctccccgt tcggcgtcgg acagaagccg
     3781 cagaagctga acgcggtttt tcattttcgt tccgcttttg ccttcatccg cgtaaatctt
     3841 cattaaccga taaccgtttc ggcggcagta ttcggcgaaa aatttccgct gggacgcgag
     3901 actgtcaagc tgctcttcct tatttgtcga aacacgacag taagccaccg cgt
//
LOCUS       DATALY010000095         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_712066,
            whole genome shotgun sequence.
ACCESSION   DATALY010000095 DATALY010000000
VERSION     DATALY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712066"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHR42_02775"
     CDS             <1..220
                     /locus_tag="VHR42_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026138.1"
                     /translation="IHLDLSHLSYHEISRKMAPVLQGKSKRKIISAVTRAAWTVFAVD
                     GLYGLAAKVRCREVEKGAADQIMGRDIS"
     gene            complement(193..552)
                     /locus_tag="VHR42_02780"
     CDS             complement(193..552)
                     /locus_tag="VHR42_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026139.1"
                     /translation="MKITFPHMGNAYVAVKVLLDTLGLDYYMPPVKNRNALQRGIANS
                     PEFMCLPFKTILSDFLQGLDHGADTILFGGGCGQCRLSYYGALEQEILRSMGYRFNYI
                     HLDLSHLSYHEISRPII"
     gene            complement(608..1570)
                     /locus_tag="VHR42_02785"
     CDS             complement(608..1570)
                     /locus_tag="VHR42_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX3026140.1"
                     /translation="MKKLIYYISKTTPKKPEDVTVGIPRAMSYHEDGKLWENFFVGLG
                     CNVILSPETNRSMLDEGVRNSSNETCLPVKVLAGHVLSLEDKADYIFIPRYVSTDKLE
                     FSCPKFCGLPDMVRMSLKKDLPIMEVAVDFNNGMKKTNESLKNVAETLKLDERFVRDT
                     FISTVKNRIRPEMEAGVLDADPEKPSIAVLGHPYMIHDGCMSLNLIKKLKSNHYQVLT
                     PDNLGFSTKKKNAYPFSNTYFYGIGYENLGSAFTFASLPSLRGIIYLTPFACGIDSLV
                     TEFIERHLKTVNESVPFMKLTVDEHTGEAGVDTRLEAFLDMIAI"
     gene            complement(1582..2556)
                     /locus_tag="VHR42_02790"
     CDS             complement(1582..2556)
                     /locus_tag="VHR42_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX3026141.1"
                     /translation="MGKDDLFLGIDVGSVSTNLVAVDRGGSVIKKLYLRTSGKPIQVL
                     TEGLKQLSSALGDAAIGGAGTTGSGRQLAGVIAGADIVKNEITAHAVAAQRVVPDVKT
                     ILEIGGQDSKIIFLKSGVVHDFAMNTVCAAGTGSFLDRQAARLEIPIEDFGKYALRSK
                     SPTRIAGRCAVFAESDMIHKQQTGHSTEDIIGGLCEALVRNYLSNLAKGKTIEEPILF
                     QGGVAANVGIVAAFEKALGQKVVIPRYYDVMGAYGAALLAKNFMEKCGGKSRFLGFKN
                     AENHFENRSIECSGCSNLCEVIEIRSNGAVVARWGDRCGKWSHQSVAV"
BASE COUNT          672 a          778 c          683 g          726 t
ORIGIN      
        1 tatccatctt gatctgagcc atctctccta tcatgaaata tcccggaaaa tggccccggt
       61 gcttcaggga aaaagcaaga gaaagatcat aagcgccgtc acccgcgcgg catggacggt
      121 tttcgcggtg gacggtttat atggcctcgc cgccaaagtc cgatgcagag aagttgaaaa
      181 gggcgccgcc gatcagatta tgggccggga tatttcatga taggagagat ggctcagatc
      241 aagatggata tagttaaacc gataacccat gcttcggaga atctcctgct cgagcgcgcc
      301 gtaatagctc aggcgacatt gtccgcagcc tccgccgaac aggatggtat ccgccccgtg
      361 gtcaaggccc tgaagaaaat cgctgagaat cgtcttaaaa ggcaggcaca taaattcagg
      421 agaattggcg attccccgct gaagggcatt tctgtttttc accgggggca tataatagtc
      481 aagaccgagg gtgtccaaca ggaccttgac cgcgacatag gcgttcccca tatggggaaa
      541 agtgatcttc atatttaagg ctcctccccc gccggaaaaa ccggcggcat ttttaacagc
      601 cggggcttca gatcgcgatc atgtcaagaa aagcctcaag ccgggtatcg actcccgctt
      661 cacccgtatg ctcgtccacc gtcagtttca taaaaggcac gctttcgttg actgttttga
      721 ggtgcctctc gataaactcg gtgacgaggg aatcaattcc acaggcaaac ggagtcagat
      781 agatgattcc ccgaagggaa ggcagcgagg caaaagtaaa cgcgcttccc agattttcat
      841 aaccgatgcc ataaaaatag gtatttgaaa acggataagc atttttcttt tttgtggaaa
      901 agcccagatt atccggagtc agcacctgat agtggttgct tttcagcttt ttgatgagat
      961 tcaggctcat gcatccgtca tgaatcatgt agggatgacc cagcaccgca atggacggtt
     1021 tttcaggatc cgcatccaga acgcccgctt ccatttccgg tctgatcctg tttttgactg
     1081 ttgagataaa ggtgtcccga acaaaccttt catccagctt cagagtttcg gccacgtttt
     1141 tcagcgactc attcgtcttt ttcatgccat tattaaagtc aacggcaact tccatgatcg
     1201 gaagatcttt tttcaggctc atcctgacca tatcgggcag cccgcagaat ttagggcagc
     1261 tgaattccag cttatcggtg ctgacatagc ggggaatgaa aatataatcc gctttatctt
     1321 cgaggctcag cacatgtccg gccagaacct tgaccggcag acaggtttcg ttgctgctgt
     1381 ttcggacgcc ctcgtccagc atgctccggt tagtttcagg cgatagaata acattgcagc
     1441 caaggccgac aaaaaagttc tcccacagtt ttccatcctc gtggtagctc atggctctgg
     1501 gaattcccac cgtcacatcc tcgggctttt ttggtgtggt ttttgaaata tagtaaatca
     1561 gttttttcaa tcttcatgct cctaaacggc gacgctctga tgcgaccatt ttccgcaacg
     1621 gtcaccccag cgggccacaa ccgctccgtt tgaacgtatt tcgatgactt cacaaagatt
     1681 tgaacatccc gagcattcaa tgctcctgtt ttcaaaatgg ttctccgcgt ttttaaagcc
     1741 gagaaaacgg cttttcccgc cgcatttctc catgaaattc ttcgcaagaa gcgccgctcc
     1801 ataagcgccc atgacgtcgt agtaacgggg aatcacaact ttctggccca gcgccttttc
     1861 aaaagcggcg acgatcccca catttgccgc gacgcccccc tggaacagga tcggctcttc
     1921 aatcgttttg cctttggcca gattgctcag ataattgcga accagcgctt cgcacaggcc
     1981 cccgataata tcctcggtgc tgtgcccggt ctgctgcttg tgaatcatat cggactccgc
     2041 gaataccgcg cagcggcccg cgattctggt cggcgatttg gagcgcaggg catattttcc
     2101 gaaatcctcg atgggaattt caagcctcgc ggcctgccgg tcgaggaacg aacccgttcc
     2161 cgcggcgcag accgtattca tcgcaaaatc atgaacaacg ccgcttttta aaaatatgat
     2221 ttttgaatcc tgaccgccga tttccaggat ggttttcaca tcgggtacca ctctctgggc
     2281 ggcgacggcg tgggcggtga tttcattttt gacaatatcg gctccggcaa tcacgccggc
     2341 cagctgtctt ccgcttcccg tcgtcccggc gcccccaatg gccgcgtccc cgagcgcgga
     2401 ggaaagctgc tttagccctt cggtcagcac ctgaatcggc ttgccgcttg tcctgagata
     2461 caatttcttt attacgctgc cgccccggtc gacagcgaca agattggtgc tgaccgatcc
     2521 gacgtcgatt ccgagaaaca aatcgtcttt tcccattttt ccctccggta ttttactgcg
     2581 gctccggttc tttgccggga atccgcatga ttacttcaat ttcataattg attatttgca
     2641 atattgcgga ttttattccc tcccgatcgc atccgacgtt agtcaaaaaa ataaagcccg
     2701 attttaaaaa cagggccgga cgggaacaaa aattgactgc tattgaatca aaacaggctg
     2761 gatctgcctg cctttcgccg cttcgagcag agccggcgca atctgatcaa aatccgccac
     2821 gatcgaggtt ggctttttta tcctgtcatc ctgtctggc
//
LOCUS       DATALY010000096        36668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_798427,
            whole genome shotgun sequence.
ACCESSION   DATALY010000096 DATALY010000000
VERSION     DATALY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 36668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36668
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798427"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VHR42_02795"
     CDS             <1..599
                     /locus_tag="VHR42_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX3026142.1"
                     /translation="ALDEGYSRIPVYEDDLDNIVGVVYVKDLLRYVGTPLSKELDIRE
                     FMRPPLFVPETKRCRELFSALTAQKLHMAVVIDEYGGTAGIITMEDLLESIVGNIQDE
                     YDHEEEEYSVIDESTYTIEGMTDLEEVSHLLGVELPEGDYDTLGGLIIDHLGRIPKEN
                     EHPSVEIDGILFTVEKVEDKRIDRVRAVKKPPVEAPQK"
     gene            complement(681..1871)
                     /locus_tag="VHR42_02800"
     CDS             complement(681..1871)
                     /locus_tag="VHR42_02800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3026143.1"
                     /translation="MRMIAVIYPNLLAAAAALLVLFNLFGRKNNAFFRSGTITVMLSV
                     NLILLLLDSAARLLDGRSGRWVTVADEIVLSLMLMVLAALAVLVVLYVDFQVFSSKAH
                     LKRTVKLMLFLLIPVMILVTANPLTGSVFRLGASNVYSGGGLLYVYAAICDAGFFVIL
                     AMLVFGRRRIEHRKLLSLLRFSAFPFIGGLLQLACPQIPIVCPCVTLSLIEVYISLQS
                     SSLTTDYLTGIFNKMHLDEHILQKVRRCSPDRTFSAIMIDLDNFKEINDRFGHKVGDD
                     ALETAAMLLKQSIGQENFLARIGGDEFLAVLNADDYPSLLKVTRRINGLFDHFNASGK
                     KVYRLSLSMGYDVYSYKRRMTKDQFLKHIDCLMYLNKRNKKKMWSDSEKAQASKSVRE
                     AVHP"
     gene            complement(1951..2673)
                     /gene="pflA"
                     /locus_tag="VHR42_02805"
     CDS             complement(1951..2673)
                     /gene="pflA"
                     /locus_tag="VHR42_02805"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HEX3026144.1"
                     /translation="MNQPKGYIHSIETFGALDGPGIRYVVFLQGCGLRCRYCHNPDTW
                     QKECGRQISAGELFSDLISYLNYIRRGGVTFSGGEPLLQAGFLTRMLQLCRERKIHTA
                     IDTAGSVELSLCREAVDLADLIILDIKALDSGDCIALTGSDNRNALRLLDYLEQTGKD
                     VWIRHVLVPGITLKREKLEKLAEYIKRFSCVKKVELLPFHKMGEYKWRELRYSYTLYD
                     VPEPAKEEIEQAKQIFKLQNLL"
     gene            complement(2768..4981)
                     /gene="pflB"
                     /locus_tag="VHR42_02810"
     CDS             complement(2768..4981)
                     /gene="pflB"
                     /locus_tag="VHR42_02810"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEX3026145.1"
                     /translation="MEFVTGNWTKSIDVRNFIQLNYQPYDGDESFLEGPTSRTEKLWE
                     KLKEMMNAESARGGVYDIDPDTISTITAHKPGYIDRELETIVGLQTDAPLKRAILPFG
                     GIRMVRTSLKAYNRSINPQVEHVFKYAKTHNDGVFDAYTDEMKKARHTGIVTGLPDAY
                     GRGRIIGDYRRVALYGVDFLIEKKHRAKDSYTFDIMDSRVIREREELSEQIRAFEELR
                     EMAASYGFDLSGPAKDTKEAVQWLYFAYLAATKEQNGAAMSIGRISTFLDVYAQRDLD
                     EGRYTESEIQEFIDHFVMKLRIIRFLRTPAYDELFSGDPTWITEALGGIGIDGRHMVT
                     KMTYRFLHTLVNLGSAPEPNMTVLWSDRLPESFKKFCANLSVKTSSIQYESDELMREK
                     FGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGRDEITGDQIGPEMAPVSD
                     GYLDYDEIVRKYDHVLDWLSRLYINTLNIIHYMHDKYCYERIEMALHDEDIIRTSACG
                     LAGFSVVVDSLSAIKYAKVRPVRDERGIATDFVIEGDFPKFGNNDPRVDEIAVNVVKD
                     FMKKLSKHRTYRESKPTLSILTITSNVVYGKKTGSTPDGRKAGEPFAPGANPMHHRDK
                     NGCVASMKSVAKLPYDFCEDGISYTFSIVPGALGREESERIENLVSLMDGYFLDGGHH
                     INVNVISREVLLDAMDHPELYPQLTIRVSGYAVNFVKLSREQQLDVINRTFHTRF"
     gene            complement(5142..6353)
                     /locus_tag="VHR42_02815"
     CDS             complement(5142..6353)
                     /locus_tag="VHR42_02815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005916208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX3026146.1"
                     /translation="MNARVPMLDLKRQYASIRHEIEPAVQSCLESGVFIQGPQVREFE
                     REAAEYLQVRHAVSVGNGTDALVIALRAAGIRAGDEVITSPFTFFATAEAIVTAGGVP
                     VFADILEDSFNLDPQSVKKKITSKTRAILPVHIFGQPAGMDELLELSAEHGLKIIEDA
                     CQAIGAEYHGKKVGGIGDAGCFSFFPTKNLGAFGDGGLITTNDDNIASVCRALREHGG
                     GRNGASAVSILGGGLPVEEQEAADPLYNPYKYYNYILGYNSRLDALQAAVLRVKLHHL
                     DEWNLRRAENAAYYRSHLENSQVIPPKAGSGVKHVWHQFAVRTPYKNQLGKYLESRLI
                     ASGVFYPVPLHLQKAFEYLGYREGDLPMAEKVSSQTVCLPIFPDLTRQELDDVIEAVR
                     SFHVAVGSNDE"
     gene            6618..7259
                     /locus_tag="VHR42_02820"
     CDS             6618..7259
                     /locus_tag="VHR42_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEX3026147.1"
                     /translation="MDCNVKTILEKKIQKTIENLKKNRMGAFYCPTSKDAVKCARGLL
                     KDGDVVTNGGSMTLREAGIIDLLKSGRYTYLDRDEPGLSPERKGEILRRAFSADCYFT
                     SANAVTEEGELYCVDGNGNRIAAMIFGPRQVIVVAGYNKIVTDLHAAEDRIRSLAAPA
                     NAARLGLNNPCVKTGSCADCRLDSRICSIYTRFGHQSNDGRITVLLVGEELGY"
     gene            7285..8211
                     /locus_tag="VHR42_02825"
     CDS             7285..8211
                     /locus_tag="VHR42_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026148.1"
                     /translation="MRYIIRRILLSVFTLFAIVTVTFFLMHLVPGGPFAGSKQLPPTV
                     LHNLEAKYGLNRPLIIQYGDYLKALCHGDLGVSVKQQGRSVSELILSCFPVSAGLGFA
                     AMVFAIVIGTALGVLSARRNGQSLDRFVRVLATIGTSIPSFVLATVTLILFGVMLRWV
                     PTYGLDTPQSYILPAFSLSLFPLSFIARLMRSSMLEVIGSDYIRAARAKGIRESRIIL
                     VHAMKNAVLPVVTYIGNLAAGILTGSFVIEKIFSIPGLGSAFVESVQNRDYPLIMGTT
                     VFYGALLMAAYLIVDFLYLAIDPRIKLSRQGD"
     gene            8213..9211
                     /locus_tag="VHR42_02830"
     CDS             8213..9211
                     /locus_tag="VHR42_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026149.1"
                     /translation="MSELSKDLESLFVPASLEERRSTRRKKSIARSRRLILQEFLRSR
                     AVTVSIGFLLLLALLAIIVPALSPYSYDRQIRGAELIAPFHSLSHPLGTDALGRDEMV
                     RLLFGTRISLAIGVTVSLLSVAIGVVYGAVSGYFGGWADSLMMRAVEIISSIPALLVI
                     ILLYVAISEPLKSFLSHHPSLFFLQNIGPALISIFITFALLFWTDTARIVRGQILKLK
                     ESDFILAARACGASHSRIIFRHLIPNSLGVILITATLNIPSAIFGEAYLSFLGLGVSP
                     PMCSLGSLANDALAGVYTYPHLLILPSALICLIILAFHRIGDGLRDTLDPTSGSEV"
     gene            9218..10207
                     /locus_tag="VHR42_02835"
     CDS             9218..10207
                     /locus_tag="VHR42_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026150.1"
                     /translation="MSNLLEIKNLCVSFPTPRGNVEAVRNVFLSLGRGEAVGIVGESG
                     SGKSTMAAAIIGLLDRPGRITDGSITFDEKKLTGLPNEELRKIRGKEIAVIFQDPSSS
                     LNPLFTVGSQIGESLRFLEGIKGRQALDKAAGLLRMVGINDPYRRLKQFPHELSGGMR
                     QRVMIAMALTGGPKLLIADEPTTALDVTIQAQILEIIRELKIKTGMSVLIITHNPGVA
                     AQTADRIAVMYGGKIIETAPCGELFRNPRHPYTVGLLQSLPGLDRGKPPIAIEGSPPD
                     PLDLSLGCPFAPRCRFCLKICLVRAPRSAKIAENHEFSCWLSDRRAGTGRITE"
     gene            10204..11175
                     /locus_tag="VHR42_02840"
     CDS             10204..11175
                     /locus_tag="VHR42_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026151.1"
                     /translation="MNEPLIRVENLKKQFPIGHWPKHDRITALDGVSLEIRPGETLGL
                     VGESGCGKTTLGRTILRLTEPDSGRIFYNGEDITGRGMAPIRQKMQMVFQDPYTSLDP
                     MMTAGNIIAEPLDIHKLCGSRQERAEKVAALLEAVGLSAGDAESYPRSFSGGQRQRIG
                     IARALAAGPSFLVCDEPVSALDLSIQAQILNLLGKLQKELRLSCLFISHDLAVVRHIS
                     DRVGVMYLGEIVEMAGTEELYSNPLHPYTKALMSAIPSICKIGTVQTKKIILTGEAPS
                     PIHPPSGCKFHTRCPAAEKICALRSPPLLENGQGHFCACHKTAIRRS"
     gene            11218..12966
                     /locus_tag="VHR42_02845"
     CDS             11218..12966
                     /locus_tag="VHR42_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3026152.1"
                     /translation="MKKLLAAAFAVLIITGFLAGCATTSAKIVATRPIRACIGAEPES
                     IDPAINQTTDGATYISHAFEGLMKTDKSGKTVPGIAESYQVSADGLTYTFHLRKDAKW
                     SDGKAVTAHDFVYSLQRVANPATASAYAYQLYYIKNASQINSQYVGSDGKPGKVKIGS
                     DGSPVRNSQGNCQPDSGGKYVSAKSDGSPVWLDDLGVKATDSHTLVITLTSPCTYFLQ
                     IAASPTLYPVRQDIVEKNPDKWTLSPDTYISDGPYIMKSWNHNSKIVFVKNPYYYDKK
                     DIVSNEIDWMLMSDSNAVLAAYKSGKLDLAEDYPTDELAGLVKSGDAKINSLLAVYYY
                     VFNCSKAPFNNPKVRQALTLAVDRSYLVKTVDKGGQTPAGAFVPFGVPDAADQTDFRK
                     TGGSYLPTTTNKETLAKAKRLLAEAGYPNGKGFPITEIKFVTNEGNQKIAEFIQSEWK
                     NNLGITVTLSNEEGSVYLSDKAAGNYGIACNGFSGDYNDPMTFLDLYTSQSGNNDAGY
                     SNTEYDRLIEQAKVTSNQKQRMATLHKAEKILMSDLPVMPIYYYTDPDLISPNLQGEV
                     HSIFGYKYLMWASVKQ"
     gene            complement(13078..13473)
                     /locus_tag="VHR42_02850"
     CDS             complement(13078..13473)
                     /locus_tag="VHR42_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026153.1"
                     /translation="MKKTLFKNRYGFDMLSGFLLMIGVAFLVSRYTWILSALLAGIVL
                     VRAFSKNSEKRKRELWVFELMMRKTAWLLKNNPLTSKLSLCRLKWSQRKEYRIVRCPK
                     CRKNLRLPKSRGKIIVTCPMCGHEFQTKT"
     gene            complement(13498..14331)
                     /locus_tag="VHR42_02855"
     CDS             complement(13498..14331)
                     /locus_tag="VHR42_02855"
                     /inference="COORDINATES: protein motif:HMM:NF018271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HEX3026154.1"
                     /translation="MKLIEVYFLYFMAYSLVGWLCETVYCSCLAGNFINRGFLNGPFC
                     PVYGAGALAILWSMNGLARNPVLIFIVGIFLTTGIEYLTGYLLETAFKTKWWDYSNKR
                     YNIHGRVCLENSILFGLMSVVLILVIHPAFSRLAAGLPDMVITWSTVLLAVYLAADLS
                     LTLTTILKLIGRLEKINTMMDAIKEKLDSSGWYNTVNIRERIEKLFEAGERIGESPLY
                     QTIQNLRDGVKKIENDNRLLQKRLIAAFPDLKSIKYPEYLNNIREQILNVKSRMPNNR
                     K"
     gene            14576..16213
                     /locus_tag="VHR42_02860"
     CDS             14576..16213
                     /locus_tag="VHR42_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026155.1"
                     /translation="MGKWKSLLRLRPFLKNYRFILSAGIAGTILSSLLSTPVPYLIGH
                     LLDKVLMGNKSYRDLYLYVGVIAVLYLLEYGVSLISKNLFVRINNSVVNELRYSVMDK
                     VIDLPMDYLSGTEKGYVQGRISECSSVGSIFSPSIISMLLSLINALFAAATMFVINYK
                     LAFVVLGLTPVFFFSAKSSTKGFMKNTKSMMESSAVLNGECFEIMNGIEDIKVLGGKR
                     QHLSKFKAKIDELVHYSVKQSKSMILFTENISLINDAGTLLILLISGLLILKGQFTIG
                     LYTSFSLYSVRVFASTQGLATLGTVLKPVCLSIERIYELLDMEDENSGRVQALESAIN
                     TIDFDQTGFRYKEDLPFVFQGVSFRLHKGDKVLLRGENGSGKTTLVKLLLGLYVPTSG
                     IIAVNGRNISLLSPESLRRRIGVVSQNIFLFRGTVLDNILYGQPEKTRQNVTDLIESL
                     GLQDYISRLANGLDTEISQNTSGVSGGQAQIIAFIRALLSQKDVIILDEPVSNVDAET
                     RNRILQILKKGNYGGILMVISHQTEGMEFLDSVIEIR"
     gene            complement(16382..16810)
                     /locus_tag="VHR42_02865"
     CDS             complement(16382..16810)
                     /locus_tag="VHR42_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013708567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX3026156.1"
                     /translation="MVEFKSFQVKSEKEFQLIRITDEFKKFVADSGVQNGFAMVVTAH
                     TTTGIMVNEDLDCLAVDIEETLDRLVPADMPYAHSHFLPTYGATGGNAPGHLKSMLTG
                     NHCLFPIKDGELILGAAQEIFFAEYDGPQLRKMTFEAVGE"
     gene            complement(16816..17667)
                     /locus_tag="VHR42_02870"
     CDS             complement(16816..17667)
                     /locus_tag="VHR42_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3026157.1"
                     /translation="MVIDVHTHPVFFNDISGDSERLLYRKLQYGLYKGRPHPMDLVMT
                     QMDYAGIDKMVLLPEDLTSINGGIPISNEEIKKLVDLKPDRFIGFASVDPHRTDAVEV
                     LDHAFKDLGLMGLKLNPSKQKFYPDEELLTPIYKKCLEYNKPILFHAGMSWEPDAPSK
                     YARPINFEDVAMNYPELRICLAHFGWPWMHETVMLLLKYPNVYTDTAMLYMDSPKDFY
                     DQLFMRNMGPQWINRNMHSQILFGSNGPRFGAQDLKKAIEELDLRPFVKKKILGENAM
                     KFIGLEG"
     gene            complement(17798..18580)
                     /gene="frlD"
                     /locus_tag="VHR42_02875"
     CDS             complement(17798..18580)
                     /gene="frlD"
                     /locus_tag="VHR42_02875"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: protein motif:HMM:NF007321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEX3026158.1"
                     /translation="MKVVGVGTVCIDYYPKLNRYYATGNGVDFVIHLSRFGMETSIVS
                     MVGTDSYGKLMIDTLGEKGVDTSHIRIREGKTAVVQMDLNGRDRVHGAFDAGVMDQFS
                     LTQDDVEFILRHDMIHVDMDCRIFELLPMFHEKGKKIVFDFSVRLKDPSVQNLLRSVD
                     YAFFSYNQKDPYILDFVKWAKSCGPQIVTVTLGPNGSLSYDGKNIYESGPMPAKVVNT
                     VGAGDSFIAGFMSGIINGKGIEESMKKGTETATKVIQIFNPY"
     gene            complement(18663..19511)
                     /locus_tag="VHR42_02880"
     CDS             complement(18663..19511)
                     /locus_tag="VHR42_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3026159.1"
                     /translation="MKNIKKVRRTPEIIGLIIVIFLFVIMMLPLFWMFSCSLKLNTDI
                     FKTPPVIFPRHPTLDPYITQLFNESFSILASLKNSMIIAVATTVIATVLSIPAAYGVA
                     RFNFKFKRAIIFIFLVTQMLPSVFTLIPNFIIFKKIGVYNTYWAPILADCTLGIPFCV
                     LMLRPYFISIPKEIEDAARIDGCGALRVFLQIVTPIVSTGIVVAVVFSFLFGYGDMVN
                     ALTFINEQSLWPVTTGIYNCVGRYGIRWNEAMAFGVMAVIPVVAIFLLMQKYVVAGLI
                     GGAVKG"
     gene            complement(19514..20404)
                     /locus_tag="VHR42_02885"
     CDS             complement(19514..20404)
                     /locus_tag="VHR42_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3026160.1"
                     /translation="MVLKKRYERIGYLFVLPALIYMCCLMIYPIFYNIALSFKDVNMF
                     NLISGDKPFVGFQNYMKVFSEPLMKTSILNTFYYTVGSILFQFVLGFLFALLFTGKFR
                     CLKPMRGLLMITYILPVTVTSILFKFMFDTNCGVINQTLLSLGLIPKAIPWLLKPTSA
                     MWATIITNIWCGIPFNMMILSAGLTSIPTDIYESAVLDGASVFQKFFYITLPLMRTTI
                     EAVLVLGFVYTFRVFDLIFVLTNGGPVNSTEVLSTYSYKRSFVEFNFSQGAAIANVLF
                     VILLVVGLIYTRLIKSDEAM"
     gene            complement(20506..21744)
                     /locus_tag="VHR42_02890"
     CDS             complement(20506..21744)
                     /locus_tag="VHR42_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3026161.1"
                     /translation="MKKLLTIFLSMLLVISVSGCAGSSTTTKKTTEKVNLQMWHYYND
                     TKSKNTIQNFCDQYNKQSKVASVKAVSLAFADFKKQLSISAAASSLPDVVMIDNCDNT
                     AYSAMGMFSDLTSFVKTWPDYEKYYDWAKKTGQYKGKIYGIPFDGNCLGIMYNKQMFR
                     DAGITSTPKTWDELRADAKKLTKSGVAGFIMCAAKNEEGTSQFDPWLWSAGGSEYHMT
                     SPDSLRALTFLYNMVKDGSMPTDIINWSQSELLSQFESGKTAMMEEGSWDLDNLKTDC
                     KFEWGCFQIPKDKYSVDVLGGENLAIVKGKNVEASFDFMKYVLKTENVVQWTKSTSQF
                     PERSDSIEDSRFSETENWKMFKDIFKTTTRSRPVDTAYPQISSGVQLALQEALSLQKS
                     PEQAVKDGQALIDQAVANRK"
     gene            complement(22811..24082)
                     /gene="uvsE"
                     /locus_tag="VHR42_02895"
     CDS             complement(22811..24082)
                     /gene="uvsE"
                     /locus_tag="VHR42_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX3026162.1"
                     /translation="MRGDEKMRIGYACLTIGTYGTGFKRCLKKSADNLVLSSIIEDNL
                     AALEKMIDYNIENSIGLFRISSDLIPFGSSPVNRLEWRKLYASRLSSIGEKIQSSGMR
                     VSMHPGQYTVINSPDGDVVGRAFADLRYHTDVLDSLGVGPENKIILHVGGVYGDKNQA
                     VKRFEDNFCRLDEAIQSRLAIENDERCYHIGDVLGIGEQLGIPVVFDNLHHAVHPDAP
                     EGDGMKWLRACAHTWGKQDGPQKIHYAQQNPGKRPGAHSSTIRIDPFLEFASKLDRGI
                     DMMLEVKDKNLSAVKCINCTAQDGKIDVLKKEWEKYKYKVLENDPSGYRAERELLKDK
                     NRYPATEFYRIIERAIETEVKPGNAEKAALHLWRYFKYQATEKEKKNFFRRLDDFRHN
                     RSGIKPVKNLLHRLSEKYEQKNLHDSYYFIL"
     gene            complement(24172..25095)
                     /locus_tag="VHR42_02900"
     CDS             complement(24172..25095)
                     /locus_tag="VHR42_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3026163.1"
                     /translation="MNRFKYITRAYRQARRIGFDDSSKIILFSDCHRGDGSWADDFSG
                     NQNLYFAALTYYYKNNYTYLEIGDGDELWKNKKISDIMSVHSDAFWQLDRFDQEGRLY
                     LLYGNHDKVKKSSSASCGFYQYYDEQQKKKIVLMPKIRFNESVVLTHRVSQKQIFLLH
                     GHQADGFNDDWWKLGRFLVRHVWKPLEMIGIKDPTSTAKNHQKKEAVEKWLEEWAERY
                     QIITIAGHTHRPVFPQPGESLYFNDGCCVHPRCITGIEIAGGKIQLIKWSVRTKQDGT
                     MYVGRDILEGPAVVDEYYNFRKDSRTDACRG"
     gene            complement(25164..26183)
                     /locus_tag="VHR42_02905"
     CDS             complement(25164..26183)
                     /locus_tag="VHR42_02905"
                     /inference="COORDINATES: protein motif:HMM:NF026101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4474 domain-containing protein"
                     /protein_id="HEX3026164.1"
                     /translation="MIMNIRMIMPLFVGAASPNTENAVLVAAVLLITVAFAAVLLAFA
                     LKPRWLIRKVDGLKRLLIWLFRRKAVNSRALEEALEDTGFTYDPQQDIFYSIKDAWQR
                     EFGYCRLYDEAAAPSGMILDSDPVYFEYGSKRWLIEFWKGQYDLTCGAEIGVFSTDIP
                     DLDIPGLFSGTFYQSVDDNNMLYLTMSLKKNGYTLFTRQEKQWWLTGFKLGEFSEPSE
                     LTLYLSVTLKNHEMCDAFIGGLNNAGYDSNEYAVHDNTVSLTFQTPHSRQPLTRIPAT
                     DHLIQMKNRLMCEEYQKITRAAQDMPSKYRLIRRKSPAMLNEIFNFRKTKQINEIFKT
                     IQKYL"
     gene            26409..26915
                     /locus_tag="VHR42_02910"
     CDS             26409..26915
                     /locus_tag="VHR42_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX3026165.1"
                     /translation="MNLTDELNQKKIEERYSLALRQTQSLLEDEHHVIANLANASALI
                     YETMSDINWAGFYLKSDGELLLGPFQGKVACVHIALGKGVCGTAAATNRTIIVEDVHQ
                     FQGHIACDCSSRSEIVIPLRQDGAVVGVLDIDSPSVGRFNQADARWLERIAQTIDESC
                     DWNSTFSF"
     gene            complement(26935..27789)
                     /locus_tag="VHR42_02915"
     CDS             complement(26935..27789)
                     /locus_tag="VHR42_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX3026166.1"
                     /translation="MKGSKLKEFLFVNMGLVLTAMGIVLFKTPNGFATGGVSGIAIII
                     NKYMPGQNVGLIMLLINIVLDVLGILMLGKDFGIKTIYSSFALSFFVWVGAKLIPLTK
                     PITGDSMLELIFAITLPAIGSAFVFSQNASTGGTDIVAKILTRYTHLHVGKTLLLADF
                     LIAFSALFTLGVRQGMYSVLGLVMKGFIIDMVLEGLYTSKKLEIITSQPQKIENYILQ
                     EMHRGITIIPAQGGYTHDEKQLLTVVLSRSQAIKLSGYIHQTDPRAFIVITTTSEIIG
                     KGFRNTDQ"
     gene            28050..28787
                     /locus_tag="VHR42_02920"
     CDS             28050..28787
                     /locus_tag="VHR42_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3026167.1"
                     /translation="MKTVSQVSKLAGISVRTLHYYDQIGLLSPETAKSGYRLYSDADL
                     EKLWRILFYRELEFPLEQIRKILSHPSYNRDEALRGQRLLLVEKKRRLENLIASIDQT
                     LKKGFEVKLLKTFDKISLEEHKKIYAREAREKYGKIYEQSEEKTSQYSEDRWNLNMKQ
                     AQSIFDGLAACMEQSPDSPEVQTLIGKWRAHITENYYECTPEILEGLGKLYLEDERFT
                     RNINKTRPGLAEFLSRAIGVYCRKLRG"
     gene            complement(28920..30629)
                     /locus_tag="VHR42_02925"
                     /pseudo
     CDS             complement(28920..30629)
                     /locus_tag="VHR42_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     misc_binding    complement(30824..31090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(31134..32231)
                     /gene="aguA"
                     /locus_tag="VHR42_02930"
     CDS             complement(31134..32231)
                     /gene="aguA"
                     /locus_tag="VHR42_02930"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010989327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEX3026168.1"
                     /translation="MKIITDSTPKRDGYRMPGEFEPHDGCWLIWPERQDNWRLGAKPA
                     QEAFKQVIAAISRFEKVRVAVGAGQYEHVRSELPPQVKIVEMSSNDCWMRDCGPVFVK
                     NKKDDIRGVNFEFNAWGGLYDGLYFPWDKDSQIAEKVCEIEEKDVYQADGFVLEGGSF
                     HCDGEGTLLTTEECLLSPGRNPQLNRAQIERKLSDYLGVEKVIWIQRGLDPNETNGHI
                     DLVCCFARPGVVVTAWTEKKSNPYYEICREAYEILSKATDARGRKLKIYRLELPESAV
                     RITADESRGVDTAVKTAPRKEGDLLPASYINYYSANGGIIVPQFGDPNDGKAIQTLSE
                     IYPDRQVVGVFAREILLGGGIIHCITQQVPR"
     gene            32380..33195
                     /locus_tag="VHR42_02935"
     CDS             32380..33195
                     /locus_tag="VHR42_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3026169.1"
                     /translation="MERLSHEEWSAINGLCLSIHLSTDTHSLRTTVLNGLKKLIPYDR
                     AFFDLAYYADGDVIFFDPVFINMSQKRVHLYFKYFQMVDYARLIFIENKSAVYRDTSL
                     LPDSLREESILFKKWMQPMGMFFAGGFSVMEKKILYGSANLFRCREHGDFTAHELEIL
                     RILNTHISKRFHYLFPSGLTQSAEATPESINFHALTSREMEVMECICEGLLNKEIADK
                     LKISEFTIKKHIGNIFRKMNVTNRYQLMSKIYHTEQDDRQQEATNLNKKPSRL"
     gene            complement(33226..33603)
                     /locus_tag="VHR42_02940"
     CDS             complement(33226..33603)
                     /locus_tag="VHR42_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX3026170.1"
                     /translation="MKIAVTTQESEIFQHFGKCPEFSLFDVKDGKIQGKTKMDSSQNG
                     HSALAQFLKNAGVDVVICGGIGGGAKQMLSSVGIELVSGVEGGVDDAVKAYLDGTLKD
                     LGGSCSHGEHEHDHNCSCENHCR"
     gene            complement(33600..34055)
                     /locus_tag="VHR42_02945"
     CDS             complement(33600..34055)
                     /locus_tag="VHR42_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HEX3026171.1"
                     /translation="MDSKIKETEKLFLQGYNCSQAVFGAFAQDVGMDRETAFKLSSSF
                     GGGMGRLREVCGAVSAMFMIAGLKYGYSLPNAGEMKAEHYKLIQQLAQKFQEQNGSII
                     CRELLGLEKPEGNPVPEKRTEDYYQKRPCVELVKSAAGIMEEFMEGQSR"
     gene            complement(34061..34432)
                     /locus_tag="VHR42_02950"
     CDS             complement(34061..34432)
                     /locus_tag="VHR42_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HEX3026172.1"
                     /translation="MPRGFKCRRVCAVPENRAFTPQRPCAATVALTVEELETLRLCDL
                     EGLEQDEASVRMNVSRATFQRTLYQARKKVAEALCSGKNIKIGGGNYELAEHRCGCRR
                     ACRSCRFENKGVPGAPLSEME"
     gene            complement(34779..36149)
                     /locus_tag="VHR42_02955"
     CDS             complement(34779..36149)
                     /locus_tag="VHR42_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX3026173.1"
                     /translation="MRLNFDTIFRQYDVRGVYGDDLTDEKAEWIGKAFGTYIKRKGET
                     KAVVGMDNRKSSPVLRQSIIKGLRSTGINVVGIGTVITPIFYYSTIDFHISSGIMVTA
                     SHNPAQYNGFKIQYAGRTLFGPELQEIKEIALKGSFEHGEGGYENCSPVEDYFRMMMG
                     KIKLGNRKLKVVADAGNGTAGEFAIKILEEIGCEVIPLYCDSDPSFPHHFPDPTRVDN
                     MRDLIRSVRENKADVGLGFDGDGDRLGVVDETGEIIWGDRIMVLYWREILPKHPGADA
                     IVEVKCSDQLVDEIKKLGGNPMFYKTGHSLIKAKMKEINAVFTGEMSGHMFFADEYFG
                     YDDAVYAAARLLRILSNTGDSLHQLLSGLPKSFATPEIRVPCKEEEKNAYVEKARVEL
                     RKFALDTIEVDGVRARFNDGWGLVRASNTGPELIVRCEGKTKEACQKIKQVISQSLRP
                     LNISVQ"
     gene            complement(36133..>36668)
                     /locus_tag="VHR42_02960"
     CDS             complement(36133..>36668)
                     /locus_tag="VHR42_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026174.1"
                     /translation="YENNDEETWHWFESRMTYCNSVLPWAMLSAYEVLQEKRYLEIGL
                     ESLDFLSQTTFRQNHFHPIGCNGWLKKGEMAAPFDQQPVEACEMLLVCLKAFELSKDE
                     KYIRWAKRCLGWYTGENSAQISLIDPDTGGCMDGITPIGPNCNEGAESLVSWLIARLA
                     WEKAIPDFKRRGDQIEL"
BASE COUNT         9266 a         9612 c         8209 g         9581 t
ORIGIN      
        1 tcgctctgga tgaggggtat tcaagaatcc cggtttatga agacgacctc gacaacattg
       61 tcggcgtcgt ttatgtcaag gatttactcc gttatgtggg tacgccgctg tcaaaggaat
      121 tggacattcg cgagtttatg agacctcccc tatttgttcc tgaaaccaaa cggtgccgtg
      181 agttgtttag cgcgctgacg gcgcagaaac ttcatatggc ggttgtaatt gacgaatacg
      241 gcggaacggc cggcattatt accatggagg atttgctgga atcgatcgtc ggcaacattc
      301 aggacgaata cgaccatgaa gaagaagaat attccgtcat agacgaatcg acctacacca
      361 ttgaaggtat gaccgatctg gaagaagtgt ctcatctgct cggggtagag cttcccgagg
      421 gtgactatga tacgctcggc ggtttgatta tcgaccatct gggccgcatt cccaaggaaa
      481 acgagcaccc cagcgttgaa atcgacggaa ttcttttcac cgtggaaaaa gtggaagata
      541 aacggatcga ccgggttcgg gccgtaaaaa agcctccggt ggaagccccg cagaaatagc
      601 cggattaatg gaaatttaca aaaaagcagg agagcctagg ctctcctgct ttttttcggt
      661 ccgttaatgt ccggattctt ttaagggtga acagcctccc ttacgctttt cgaggcctgt
      721 gctttctccg aatcagacca catctttttt ttgtttctct tattcagata catcaagcag
      781 tcgatatgct tcaagaactg atcttttgtc attcttcgct tataagaata aacatcatat
      841 cccatactca ggctaagacg ataaactttt ttaccggatg cgttgaaatg atcaaaaaga
      901 ccgttgatcc tccttgttac tttcagcagc gacggataat cgtcggcgtt tagaacagca
      961 aggaactcat cgcccccaat ccgggccaga aagttttcct ggcctatgct ctgcttcagc
     1021 agcatggcgg cggtttccag cgcgtcatcg ccgactttgt ggccgaacct atcgttaatt
     1081 tccttaaaat tatccaaatc aatcatgatt gctgaaaagg tacggtccgg cgaacaacgt
     1141 ctgactttct gtaatatatg ctcatcaaga tgcatcttat taaaaattcc ggtcagatag
     1201 tcagtcgtca ggctgctgct ctgcaggctg atgtaaactt ctatcagaga cagggtcaca
     1261 cacgggcaaa caatgggaat ttgcgggcag gccagttgaa gaaggccgcc gatgaacggg
     1321 aaagcggaaa accgcagcag tgacaacagt tttctatgct cgatccgtct gcggccaaac
     1381 acaagcattg ctaaaattac aaaaaagccg gcatcacaga tagctgcata gacataaagc
     1441 agtccgccgc cggagtaaac attggacgct cctaatctga aaacgcttcc ggtcagaggg
     1501 ttcgccgtaa ccagaatcat caccggtatc agcagaaaga gcatcagttt taccgttctt
     1561 tttaaatggg ccttgctgct gaaaacctga aaatccacat agagcaccac cagaacggca
     1621 agcgccgcga gcaccatcag catcaagcta agaacaattt cgtcggccac agtgacccag
     1681 cgccccgatc tgccgtccaa caatctggcg gcgctgtcga gcagcagcaa aattaaattt
     1741 accgacagca tgacggtgat cgtgccgctt cggaaaaacg cattgttttt tctgccgaac
     1801 aaattgaaaa gaaccagcag cgcggccgcg gccgccaata agttcggata aattaccgca
     1861 atcatcctca taagaaaccc ccggtttacc acttatttca ggcaaaagaa cagggtggtt
     1921 tgtatttcaa cacgaacata tttggaatga ttatagtaga ttttgaagtt taaatatctg
     1981 ttttgcctgc tcgatttctt ccttggccgg ctccggaaca tcatacaggg tataagaata
     2041 ccgcagttcc ctccacttat actcacccat tttatgaaac ggcagcaatt ctactttttt
     2101 tacacaggaa aacctcttga tatattccgc aagcttttcc agcttttcgc gcttaagcgt
     2161 tatgcccggg accagcacat gacggatcca aacatctttt cccgtttgct ccagataatc
     2221 cagcaggcgc agggcattcc ggttgtcgga accggtcagc gcaatgcagt ccccggagtc
     2281 aagcgctttg atatccagaa taatgagatc ggcaagatct actgcttccc tgcaaagaga
     2341 aagctccacc gatcccgctg tgtcaatcgc ggtgtgaatc ttcctctccc ggcaaagctg
     2401 cagcatccgg gtcagaaaac cggcctgcag aaggggctcg ccgcccgaaa aggtaacccc
     2461 gccccggcgg atataattta aatagctgat cagatcggaa aacagctccc ctgcgcttat
     2521 ctgcctcccg cattccttct gccaggtatc cggattatga cagtaacggc agcgaaggcc
     2581 acagccttgt aaaaacacga cataacggat gccgggcccg tcaagcgcgc cgaatgtttc
     2641 gatggaatgg atgtaacctt tgggttgatt catggatgat tctcctcgca aaccgggctt
     2701 ttcagcgatt tggcgggtta agccggatgt ctggtaaaat tattttgagc agttcctatt
     2761 ttcaggatca gaaccgggta tggaaggttc ggttaatgac atcaagctgc tgttcgcgcg
     2821 aaagcttgac aaaattgacg gcatatcccg aaacccgtat cgtcagctga gggtaaagct
     2881 ccgggtgatc catagcgtca aggagcacct cccggctgat tacatttaca tttatatgat
     2941 ggcccccgtc aaggaaatat ccgtccatca gagagaccag attttcgatc cgctcgctct
     3001 cctcccggcc cagcgcgccg ggaacaatcg agaaagtata ggagatcccg tcctcgcaga
     3061 aatcgtaggg cagcttcgcc accgatttca tcgaggccac acagccgttt ttatcgcggt
     3121 ggtgcatcgg attggccccc ggagcgaaag gttcacctgc ttttcttccg tccggcgtcg
     3181 atcccgtttt tttgccgtaa accacgttgg aagtaatggt caggatagaa agcgtcggtt
     3241 tgctctcccg ataagtgcgg tgtttggaca gctttttcat gaagtctttc accacattga
     3301 cagcgatttc atccactctg gggtcattgt ttccgaactt gggaaaatcc ccctcgatca
     3361 caaaatcagt ggcaatacct ctctcgtccc gaaccggtct tacctttgca tatttgattg
     3421 ccgacagtga atcaaccacc accgaaaaac cggcaagtcc acaggccgag gttctgataa
     3481 tatcttcgtc gtgcaaagcc atctcaatcc gctcatagca gtatttgtca tgcatgtaat
     3541 ggataatatt cagcgtgttg atatagagtc tggagagcca gtccagaaca tggtcatatt
     3601 ttctaacaat ctcgtcgtaa tcgagatatc cgtccgatac aggggccatc tccggcccga
     3661 tctgatcgcc cgtaatttcg tcacgccctc cgttaatggc gtaaagcagc gctttggcaa
     3721 gatttgccct cgccccgaaa aactgcatct gtttgccgat tctcatagca gagacgcagc
     3781 aggcgattcc ataatcgtcg ccgaattttt ccctcatcag ctcgtcgctc tcatactgaa
     3841 ttgaggacgt tttgactgaa agattcgcgc agaacttttt aaagctttcc ggaagcctgt
     3901 cgctccagag caccgtcata ttgggttccg gggccgagcc gagattgaca agcgtatgca
     3961 gaaaccggta agtcattttg gttaccatat gcctgccgtc aatgccgatt cctcccagtg
     4021 cttccgtaat ccaggtcgga tctcctgaaa acagctcatc ataggccggt gtccgaagaa
     4081 accggatgat tctcagtttc ataacaaaat ggtcaataaa ttcctggatt tcactctccg
     4141 tatagcgccc ttcatccaga tcccgctgcg cataaacatc aagaaacgtt gagattcgtc
     4201 caatcgacat ggcggcgcca ttctgctctt tggtcgcggc aagataagca aaatacaacc
     4261 actgaaccgc ttctttggta tcttttgcgg ggcccgacag atcaaatccg taagaagcag
     4321 ccatttcacg cagctcttca aacgcccgga tttgctcaga cagctcttcc ctctcgcgga
     4381 taacgcggga gtccataata tcaaaggtat agctgtcttt cgcgcgatgt tttttctcaa
     4441 tcagaaaatc aacgccataa agggcgaccc tgcggtaatc gccgataatt ctgcctcggc
     4501 cgtaggcatc gggcaggccg gtaacgatcc cggtatgacg cgcttttttc atctcatcgg
     4561 tatacgcgtc gaagaccccg tcattatggg tcttcgcata tttgaaaacg tgctcgacct
     4621 gaggatttat cgatctgttg taagccttca gcgaagttct caccatccgt atcccgccga
     4681 agggcaggat agccctcttg agcggagcgt cggtctgtag cccaacgatg gtttcaagct
     4741 cccggtcaat ataaccgggt ttgtgcgccg tgatcgtcga tatggtgtcc gggtcgatgt
     4801 cataaacgcc gccgcgggcg ctctccgcat tcatcatttc tttaagtttt tcccagagct
     4861 tttcggtccg tgacgtgggg ccctctaaaa acgactcatc cccgtcatag ggctgataat
     4921 tcagctgaat aaaatttctt acgtcgattg atttcgtcca atttcctgtg acaaattcca
     4981 tctatcctgc tccctgtttt tgttttttct tccgacaaac agctcatcct gtcaaaatac
     5041 attcttttca tgtaagaaaa aagaaattgt aacataattt tatttcatat taactcaatt
     5101 tccaatttat gtcaatccaa aatttcgaat attttctgct gtcattcatc gttactaccg
     5161 accgcgacgt gaaaagacct gaccgcctcg ataacatcgt cgagctcctg ccgcgtcaaa
     5221 tccggaaaaa ttggcagaca aaccgtctgt gacgacacct tttccgccat gggaaggtct
     5281 ccctcccggt aacccaggta ctcaaacgcc ttttgaagat gaaggggaac ggggtaaaaa
     5341 acccccgagg cgatcagccg ggattcaaga tatttcccca attgattttt atagggtgtc
     5401 cgcacggcaa attgatgcca gacatgcttc actcccgatc ccgctttcgg cggaattacc
     5461 tgagagtttt caagatgact gcggtaataa gccgcatttt ccgcccggcg caggttccat
     5521 tcatcgagat ggtggagctt gacgcgcaga accgccgcct gcagcgcatc gagccttgag
     5581 ttatacccga gaatatagtt ataatatttg taaggattat aaaggggatc ggccgcttcc
     5641 tgttcctcaa ccggcaaacc tccgcccaga atgctgacgg ccgacgcacc gttgcgccct
     5701 ccgccatgct ccctcagcgc ccgacagacg gatgcaatat tgtcgtcatt tgtggtgatc
     5761 aggcccccgt ctccgaaagc gccaaggttt ttggtcggaa aaaaggaaaa acagcccgcg
     5821 tcaccgattc cgcctacttt ttttccgtga tactccgcgc ctatcgcctg acaggcgtct
     5881 tcaatgatct tcagcccatg ttcggcgctc agctccagaa gttcgtccat tccggcgggc
     5941 tggccaaaga tatgaaccgg cagaatagcc ctggtttttg aagtgatttt cttttttacg
     6001 ctttgagggt caagattaaa gctatcttca agaatatcgg cgaacaccgg cacaccgccg
     6061 gccgttacaa ttgcctccgc cgttgcaaaa aaggtaaagg gggatgtaat tacctcgtcc
     6121 cccgcccgga tacccgccgc tctgagcgca atgacaagcg cgtcggtccc gttccccacg
     6181 gagaccgcat gtcgaacctg cagatactct gccgcttccc tctcaaactc cctgacctga
     6241 gggccctgaa taaacacgcc gctttccaga cagctctgaa ccgccggctc tatttcatgc
     6301 ctgatagagg catattgtct ttttaaatcc agcatcggga cacgagcatt cattccgttt
     6361 tcctatcagc cgccgccgct gcggcattcc gacgccttcc tgcggccgaa tttggaagac
     6421 tgccctgttt ttatgatgta attaattata cgatgcatgg gccccgccgt caaatcatgt
     6481 attgagaatt tctcataaaa acaggctttc aaagccccca aaacaactct tttttcgcgc
     6541 tggtcaatca attcttgaca ataaccgaga accgtgtaat atgttttgta gaacaaaaag
     6601 gagaggagtc gtttcaattg gactgcaatg tgaaaaccat tcttgagaaa aaaatccaaa
     6661 agacaataga gaatctcaag aaaaaccgta tgggagcctt ctactgccca acctcgaaag
     6721 acgcagttaa atgcgcgcgg ggtctgctga aagacggtga tgttgtcacc aacggcggtt
     6781 ccatgaccct gagagaagcc ggtatcatcg acctgctcaa aagcggtaga tacacctatc
     6841 tcgaccggga tgaaccgggc ctgagcccgg agcgcaaagg cgaaatactc aggcgcgcgt
     6901 tttccgcgga ctgttatttc accagtgcca acgccgtcac cgaagagggt gagctttatt
     6961 gcgtggacgg gaatggcaat cgaatcgccg ctatgatttt tgggccgcgg caggtaattg
     7021 tcgtagcggg ttacaataaa atcgtcactg atttacacgc cgcggaggat cggatcagaa
     7081 gtctggccgc ccccgccaac gccgcacggc tgggtttgaa caatccctgc gtcaaaacgg
     7141 gctcctgcgc cgactgccgt ctggattcaa ggatctgctc gatttacacc cgattcggcc
     7201 accagagcaa cgatggacgg atcaccgtcc tgctggttgg ggaagagctg ggctactgat
     7261 cagaaaggcc ggggaggaaa ccgtatgcgg tatatcatca gacggatact gctttccgtc
     7321 ttcacgctgt ttgccattgt gaccgtcacc ttttttctca tgcacctcgt cccgggcgga
     7381 ccatttgccg gcagcaaaca gcttccgccc acagttttac ataatctgga ggcaaaatat
     7441 ggcctcaacc ggcctttgat catccaatac ggtgattatc tcaaggcctt gtgccacggt
     7501 gatctcggtg tttcggtcaa acagcagggc agatcggtca gcgaactgat tctatcctgc
     7561 tttccggtct ccgcaggtct gggcttcgcc gctatggtct tcgccatcgt catcggaacc
     7621 gcgctcggcg tgctctccgc ccggcgaaac ggtcaaagcc ttgaccgttt cgtcagagtt
     7681 cttgcaacga tcgggacatc catcccatcc tttgtgcttg cgacggttac cctgattttg
     7741 ttcggcgtta tgctacggtg ggttccgact tacggtctgg acacgccgca aagttatatt
     7801 ctgccggctt tctccctgtc gctcttccct ctgagcttta ttgcacggct gatgcgctca
     7861 agcatgctcg aagtcatcgg cagcgactat atccgggccg cgagggccaa agggattcgg
     7921 gaatcgcgga ttatacttgt ccacgccatg aaaaacgccg tcttgccggt cgtcacctat
     7981 attggaaatc tggcggcggg cattctgacc ggcagctttg tcattgaaaa aatattttcg
     8041 attcccggtc ttggcagcgc ctttgtcgaa agcgtgcaaa acagggatta cccgctgatt
     8101 atgggaacca ctgtatttta cggcgccctg ctgatggccg cctatctgat cgtggatttt
     8161 ctttatctgg caatcgaccc gcggatcaaa ctcagccggc agggggatta agatgtctga
     8221 attatcaaaa gacctggaaa gcctgttcgt ccctgcatca ttggaggaac gccgctccac
     8281 ccggaggaaa aagagtatcg cccgcagccg gcggctgatc ctgcaggaat tcctacgcag
     8341 cagggccgtg acggtttcga tcgggtttct ccttttgctt gctctgctcg ccatcatcgt
     8401 gccagcgctc tctccctatt cctatgaccg gcaaatcagg ggagcggaat tgatcgcccc
     8461 ctttcattca ctgtctcatc cgctcggtac ggacgcactg ggccgggatg agatggtacg
     8521 tcttctgttt gggaccagaa tttctcttgc gatcggcgtt accgtgagcc ttctatcagt
     8581 tgcaatcggg gtcgtctatg gcgcggtttc gggttacttc ggcggctggg ccgacagcct
     8641 gatgatgcgc gcggttgaaa ttatctcatc catcccggcg ctgctcgtca tcatcctgct
     8701 ttatgtcgcc atcagcgagc cgttaaaaag ctttctcagc caccatccct ctcttttctt
     8761 cctgcaaaac atcgggcccg cgctgatcag cattttcatc accttcgcgc tgcttttctg
     8821 gaccgacacg gcccggatcg tccgcggtca aattctaaaa ttgaaagagt cggattttat
     8881 tctggcggcc agagcctgcg gcgcctcgca cagccggatc attttcaggc acctgattcc
     8941 caacagcctg ggggtcattc ttattaccgc gactctgaat atcccctccg ccatttttgg
     9001 agaagcctat ctcagctttt tggggctggg cgtttcaccg cctatgtgtt ctctcggttc
     9061 cctcgccaat gacgctcttg ccggtgttta cacctaccct catctgctaa tactgccatc
     9121 cgccctcatc tgcctgatta ttctggcttt tcatcggatc ggcgacggac ttcgcgatac
     9181 gcttgaccca acttcgggaa gcgaggtgta atcgggcatg tcaaatttac tggaaattaa
     9241 aaatttatgt gtgtccttcc ccactccccg gggcaatgtc gaggcggtca gaaatgtttt
     9301 cctttcgctg ggcagagggg aagcggtcgg aatcgtcgga gaatccggtt ccggcaagag
     9361 cacgatggct gcggcgatca tcggcttgct cgacagaccg ggccgcatca ccgacggcag
     9421 cataacgttt gacgaaaaaa agctgaccgg gcttccgaac gaggagctca gaaaaatccg
     9481 gggcaaagaa atcgccgtga tttttcagga cccctcgtcc agcctgaacc ctctgttcac
     9541 cgtcggcagc caaatcggcg agtccctgcg gtttctcgaa ggtatcaagg gcaggcaggc
     9601 gctggacaag gccgccgggc tgcttagaat ggtaggaatc aacgacccat atcggcgttt
     9661 aaagcaattt ccccacgaat tgtcgggcgg gatgcggcaa agggtgatga tcgccatggc
     9721 cctgacaggc gggccaaagc tgctgattgc cgatgagccg acaacagcgc ttgacgtaac
     9781 catacaggcc cagattctgg agatcatacg ggaattaaaa ataaaaaccg ggatgtccgt
     9841 tttgataatt acccataacc cgggcgtggc cgctcagaca gccgacagaa tcgccgtgat
     9901 gtatggcgga aaaattatcg aaaccgcgcc ttgcggagaa ttgttccgca acccaagaca
     9961 tccttacacc gtagggctgc tccagtcatt gcccggcctt gaccgcggca agccgcccat
    10021 cgcaatcgag ggctctcccc cagacccgct tgatctttct ctcggctgcc catttgcgcc
    10081 aagatgccgg ttctgcctca aaatctgtct ggttcgggca ccccgctccg ccaaaatcgc
    10141 ggaaaatcac gaattctcct gctggctgtc tgaccggcgg gccggaaccg ggaggataac
    10201 agagtgaatg agcctctaat tcgtgtcgaa aatttaaaaa agcagtttcc gataggtcat
    10261 tggcccaaac acgaccggat caccgcgctg gacggggttt ctcttgaaat caggcctggg
    10321 gagactttgg gccttgtggg tgaaagcggc tgtggaaaaa ccactctcgg gcggaccatt
    10381 ctgcggctga ccgagccgga ttcaggcaga attttttaca atggggaaga catcaccggc
    10441 cgcggcatgg ctccgatcag acaaaaaatg caaatggttt ttcaggaccc ctatacctct
    10501 ctcgacccaa tgatgacggc gggcaatatc atcgccgagc cgctggacat ccacaaactg
    10561 tgcggcagca ggcaggaacg cgctgaaaaa gttgccgccc tgctggaagc agtaggatta
    10621 agcgccggcg acgccgaaag ctatccccgt tccttctccg gaggacagcg gcagcgtatc
    10681 ggcatcgcga gagctctcgc ggccgggcca tcttttcttg tctgcgacga gcctgtctcg
    10741 gcgcttgacc tttcgatcca ggcccaaatt ttaaacctgc tcggcaagct tcagaaagag
    10801 ctgcggctga gctgcctgtt tatttctcac gaccttgcgg tagtccggca tatcagcgac
    10861 agagtcggcg tgatgtatct tggtgaaatt gtggagatgg ccggcacgga agaactctat
    10921 tccaacccgc tgcaccctta cacaaaggca ctgatgtccg caatcccgtc aatttgcaaa
    10981 atcggaaccg ttcagacgaa aaagatcatt ctgactgggg aagccccttc tccgatccac
    11041 cccccctccg gctgcaaatt ccatacccga tgccccgcgg ccgaaaaaat ttgtgccctg
    11101 agatctccgc ctcttcttga aaacgggcag ggacacttct gtgcctgcca taagacggca
    11161 atcaggagat catgacaatt gacaactgcg acagaatacc aacgaggagg aaaatacatg
    11221 aaaaaacttt tggctgccgc ttttgccgtg ctgataatca ccggctttct ggctggctgc
    11281 gcaacaacat ccgccaaaat tgtggctacc cgccccattc gcgcctgtat cggcgccgaa
    11341 ccggaatcca tcgacccggc tatcaaccaa actaccgacg gggcgaccta tatttcccac
    11401 gcgtttgaag ggctgatgaa aaccgacaaa tccgggaaga ccgttccggg aatcgcagaa
    11461 agctatcagg tcagtgccga tggtctgacc tatacgtttc atttgagaaa agacgccaag
    11521 tggagcgacg gaaaagcggt caccgcccat gatttcgttt actccttaca gagggtcgcc
    11581 aaccccgcaa ccgcctcagc ctatgcgtat cagctgtatt acataaaaaa tgcctcccag
    11641 atcaacagcc agtatgttgg cagcgacgga aaacccggta aggtgaaaat cggttccgac
    11701 ggcagccccg tcagaaattc tcagggcaac tgccagcctg actccggagg gaaatatgtc
    11761 tctgcaaaaa gcgacggctc ccctgtctgg ctcgacgacc tcggtgtcaa agcaacagac
    11821 agccatactc ttgttatcac tcttacctct ccctgcactt actttttaca aatagccgcc
    11881 tccccgacac tttatcccgt ccggcaggac atagtcgaaa aaaatcccga caagtggaca
    11941 ctcagcccgg atacttatat cagcgacgga ccttacatca tgaaatcctg gaatcacaac
    12001 agtaaaattg tttttgtcaa aaatccttat tattatgata aaaaagacat cgtttcgaat
    12061 gaaatcgact ggatgctgat gagcgattcc aacgctgttc tcgccgccta taaaagcggc
    12121 aagctggatc ttgccgaaga ttacccgacc gacgagcttg ccggtcttgt gaaaagcggc
    12181 gacgcaaaaa tcaacagcct tctcgccgtt tactactatg ttttcaactg ctcaaaagct
    12241 cctttcaaca atccgaaagt ccgtcaggcg ctgactctgg ccgttgaccg aagctacctc
    12301 gtcaagacag tggataaggg cggtcagact cccgcgggag cctttgtccc cttcggcgtt
    12361 cccgatgcgg ccgatcagac ggatttccgc aaaacaggcg gaagctatct ccccaccacg
    12421 acaaacaaag aaactctcgc gaaagcaaag cggctcctgg ccgaggccgg atacccgaac
    12481 ggcaaggggt ttcccatcac agaaatcaaa tttgttacca acgagggcaa ccagaaaatt
    12541 gctgaattca ttcaatcgga atggaaaaac aaccttggca ttaccgtaac tctttccaac
    12601 gaggaaggct ccgtttattt gtccgacaag gcagccggaa attatgggat tgcctgcaac
    12661 ggtttcagcg gcgactataa cgacccgatg actttccttg atctctatac ctcccagagc
    12721 ggcaacaatg acgccggata ttccaacaca gagtacgatc ggctcatcga acaagccaaa
    12781 gtaacgtcaa accaaaaaca gcggatggca acgctgcaca aggctgaaaa aatcctgatg
    12841 tccgatctgc ccgtcatgcc catttattat tacaccgatc cggatcttat cagccccaac
    12901 cttcagggtg aagttcactc gatatttggg tataaatacc tgatgtgggc gtcggtaaaa
    12961 caatagcgtc gcaacgcgga aacaagaaat cctttttata caaagacggc ggttatgggt
    13021 tgccccataa ccgccgtctt ttaaacactc ttatctgtaa acttacccgc cgtattctca
    13081 ggtctttgtt tgaaactcat gcccgcacat cgggcaggtc acaataatct tgcctctgct
    13141 ttttggcagc cgcagattct tcctgcattt cgggcaccgg acgatccggt attcctttct
    13201 ctggctccat ttcagcctgc agagtgatag cttgctggtc agcgggttgt ttttcagcag
    13261 ccaagcggtc tttctcatca tcagctcaaa gacccatagc tcccttttcc gcttttcaga
    13321 gtttttcgaa aaagctctta ccagaacaat acctgccagc aacgctgaca gaatccaggt
    13381 atatctggaa accaaaaagg ccacgccaat catcaacaga aatcccgaga gcatatcaaa
    13441 tccataccgg tttttaaaca aagtcttttt catcagcttc ctccgagcgc gcggctctca
    13501 tttcctattg tttggcatcc tgctcttaac attaagaatc tgttctctga tattattcag
    13561 atattccgga tattttatcg actttaaatc cgggaaagcc gcaatcagcc gcttttggag
    13621 caatctatta tcgttttcaa tctttttaac gccatctctg aggttttgaa tcgtctgata
    13681 aagcgggctc tctccgatcc tttcgcctgc ttcaaacaat ttctcgatac gctccctgat
    13741 attgaccgtg ttataccagc cggaactgtc gagtttctct ttgatggcat ccatcatcgt
    13801 gttgattttt tccaggcggc cgatcagttt caaaatggtc gtaagcgtca gactcaaatc
    13861 agcagcaagg taaaccgcta aaagcaccgt cgaccacgta ataaccatat ccggcaatcc
    13921 cgccgccaac ctgctgaaag cgggatgaat cacaagaatc agcacaaccg acatcagccc
    13981 aaacaaaatc gagttttcca ggcagaccct tccgtggatg ttgtagcgtt tattggagta
    14041 atcccaccat ttggtcttga acgcagtctc cagcagatag cctgtcaggt attcgatccc
    14101 tgtcgtaaga aatattccca cgataaagat cagaaccggg tttcgggcaa gcccattcat
    14161 tgaccagaga attgcaagcg cacccgcacc ataaaccggg caaaaaggtc cattcaaaaa
    14221 gcccctgttt atgaaatttc cggccaggca cgagcaataa acggtctcac acagccaacc
    14281 aactaaactg taggccataa agtagagaaa atagacctcg attagtttca ttctgaccac
    14341 cgtttccatg ccgactgacg tcttttttct tattataaca cattttaaag atcaatgtta
    14401 ccgttccggc ttataccgga cgatcaaaaa cagacacttc ttgtttgccc tcccggtcgc
    14461 agaaagctgt ggaccctcaa gcaaaataaa aatacagtct tgtcctctgc ccgtagatgc
    14521 ggtagactaa attgtatgtc aaataattat gcgggcatta aaggatatga tttgattggg
    14581 aaaatggaaa agccttttaa ggctcaggcc ctttttaaaa aattaccgtt tcatcctgtc
    14641 tgccggaatt gcgggaacca ttctgagctc cttgctgtcg accccggttc cttatctaat
    14701 cgggcatctg ctcgacaaag ttttgatggg aaacaaaagt taccgggatc tttatctgta
    14761 tgttggcgtc atcgctgttc tgtatctatt ggaatatggt gtttcgctga tttctaaaaa
    14821 tttgtttgtc cgcatcaata attcggtagt gaatgaactg cgatattccg tgatggacaa
    14881 ggtgatagat ttaccgatgg actatctttc cggcaccgaa aaaggatatg ttcagggaag
    14941 aatctcggaa tgcagctcgg ttggcagcat tttttccccg tcgattatca gtatgcttct
    15001 cagcctgatc aatgcgctgt ttgcggctgc caccatgttt gtaatcaatt acaagctcgc
    15061 tttcgtggtt ttggggctga caccagtctt tttcttttca gcgaaatcct ctacaaaagg
    15121 atttatgaaa aacacaaaaa gcatgatgga gtccagcgcg gttttaaacg gcgaatgctt
    15181 tgaaattatg aacggcattg aggacatcaa ggtgttgggt ggaaagagac agcacctctc
    15241 aaaattcaag gcaaagattg atgaactggt acattacagt gtgaagcaaa gcaaatccat
    15301 gattctgttt accgaaaata tcagtctgat taacgatgcc ggaacccttt tgattcttct
    15361 gatttccggt ctgctgatat tgaaagggca gtttaccata ggcctataca cctcattttc
    15421 attatacagt gtgagggtat ttgccagcac gcagggactt gcaacgctgg gcaccgtatt
    15481 aaagcccgtc tgtctgagca tcgaaagaat ttatgagctt ttggatatgg aggatgagaa
    15541 cagcggaaga gttcaagcgc tggaatccgc catcaataca atcgattttg atcagacggg
    15601 attccggtat aaagaagacc tgccctttgt gtttcaaggg gtcagttttc gacttcataa
    15661 gggcgacaaa gtacttctgc ggggtgaaaa cggctccgga aaaacaacgc ttgtaaagct
    15721 gctgctgggt ctttatgtcc cgacatccgg tatcatcgcc gtcaacggac ggaacatatc
    15781 cctgctgagc cccgaaagcc tgcggcgccg gattggcgtc gtctcgcaaa acatcttttt
    15841 gtttcgggga accgttctgg acaatatcct gtacgggcag cctgaaaaga cccgtcagaa
    15901 cgtaaccgat ctgattgaaa gcctgggatt acaggattat atcagccggc tggcaaatgg
    15961 gctggatacg gaaatcagtc aaaacacatc cggcgtatcc ggggggcagg cgcagatcat
    16021 cgctttcatt cgagcgctgc tttcccaaaa ggatgtgatc attctggatg aaccggtctc
    16081 gaatgtagac gcggaaaccc gtaaccggat tcttcagatt ctgaaaaaag ggaactacgg
    16141 cggaatttta atggttatct cacatcagac ggaaggaatg gagtttttag acagcgttat
    16201 tgagatccgg taaaaccgtc ggtcttccca cttatttcag acgggaagct ctttcttttc
    16261 atgttttggc gtctttaata aaaaagaaaa aagcggggag ggaaccgtct ggttccctcc
    16321 ccgcttatcc gtgtaaaatc atttgttcct tcgccgacgg gtaagccggc gatacagttg
    16381 attattcacc caccgcttcg aatgtcattt tcctcagctg aggcccgtcg tattccgcaa
    16441 agaatatttc ctgggctgcc ccaagaatca gttccccgtc tttgatcggg aacaggcaat
    16501 ggttgcctgt cagcatagac ttgagatggc ccggcgcatt gccgccggtc gctccgtaag
    16561 tcggcagaaa atgggagtgg gcgtagggca tatcggccgg aaccagtctg tcgagggttt
    16621 cttctatatc gaccgccaga cagtccagat cctcattgac catgattccc gttgtagtgt
    16681 gcgcggtcac aaccatggca aaaccgtttt gaacgccgga atcggcgaca aatttcttaa
    16741 attcgtccgt gatacggatc agctggaatt ctttttcgct tttgacttga aaacttttaa
    16801 actctaccat cacatttaac cctccagacc aataaatttc attgcgtttt caccaaggat
    16861 tttcttcttg acaaacggtc tgaggtctaa ctcctcgatc gctttcttta aatcctgcgc
    16921 gccgaaacgc gggccgttgg agccgaaaag aatctggcta tgcatatttc tgttaatcca
    16981 ttggggcccc atattacgca taaacagctg atcataaaaa tccttcgggg aatccatata
    17041 caacatcgcg gtatcggtgt agacattcgg atatttcagg agcagcatga cagtttcatg
    17101 catccagggc catccgaaat gagcaagaca aattctcagc tcaggataat tcatcgccac
    17161 gtcttcaaaa ttaatgggcc tggcatattt tgacggagcg tcgggttccc aactcattcc
    17221 cgcgtgaaag agaatgggct tgttgtattc aaggcacttc ttataaatcg gtgtgagtaa
    17281 ttcctcatca gggtaaaatt tctgcttgga tggatttaat ttcagcccca taaggcccag
    17341 gtctttaaat gcatgatcca aaacctcaac agcgtctgtc cgatgggggt ctacgcttgc
    17401 gaatccgata aaccggtccg gcttgagatc gacaagtttc ttgatctctt cattggaaat
    17461 cggaattcct ccgttaatcg atgtgaggtc ttccggcagc aaaaccattt tgtcaattcc
    17521 cgcataatcc atctgcgtca tgactaaatc catcggatga ggccgcccct tataaaggcc
    17581 gtactggagc tttctgtaaa gtaaacgctc tgaatccccg ctaatgtcat taaaaaaaac
    17641 cggatgcgta tgtacatcaa taaccatagt cctccgtcac ctttcttctt gtttattttc
    17701 aatcctatct ctatccatca caataattga tctggttgtg atgggtagag tatggttgta
    17761 acccgtggca aaatgccata atctttattt atcgtgtcta atagggatta aaaatctgta
    17821 tgactttggt cgctgtctcc gttccttttt tcatgctctc ttctatccct tttccgttaa
    17881 tgatgccgct catgaatccc gcaatgaagg aatccccggc gcctaccgtg ttaaccactt
    17941 tggccggcat cggaccgctc tcatagatgt tttttccgtc gtaggaaagg ctcccgtttg
    18001 ggccgagtgt caccgtcaca atttgcggac cgcagctttt cgcccatttc acaaaatcaa
    18061 gaatatatgg atctttctga ttgtaagaga aaaaggcgta gtcaacactt cgcagcaggt
    18121 tctgcacgct tggatctttc agtcggaccg agaagtcaaa gacgatcttc tttccctttt
    18181 catgaaacat cggaagcagc tcaaaaattc tgcaatccat atccacatga atcatgtcat
    18241 gtctgagaat aaattcgacg tcatcctgtg tcagggaaaa ctgatccatt actcccgcgt
    18301 caaacgcgcc gtgcacccga tcccgcccgt ttaaatccat ctggacgaca gctgtttttc
    18361 cctcccgtat gcgaatgtgt gaggtgtcaa cgcccttctc gccgagcgta tcaatcatca
    18421 gtttgccgta actatccgtg ccaaccatac tgacaattga cgtttccatt ccaaaccgac
    18481 tgagatgaat gacaaaatcc actccgtttc ccgttgcgta ataccggttg agctttggat
    18541 aatagtcgat gcaaaccgta ccgacaccca cgactttcat ttcacatcac cctttgtctc
    18601 agattgaaac ccggctcata aaaccggatt gtttacttcc ggactgcaaa ctgttttggc
    18661 tgtcaccctt tgaccgcccc gccgatcaac ccggccacca catatttctg catcagaagg
    18721 aatatggcga ctacgggtat aacggccatg actccaaagg ccattgcctc gttccacctg
    18781 attccatatc tgccgacgca attataaata cccgttgtca caggccagag actttgttcg
    18841 ttgataaagg tcagcgcatt caccatatcg ccgtacccga aaaggaagga gaatacaact
    18901 gccaccacaa ttcccgtgga aacaatcggc gtaacaattt gcagaaagac ccttaatgcg
    18961 ccgcatccgt cgattctcgc cgcgtcctca atttctttcg gaatactgat aaaatacggg
    19021 cgcagcatca aaacgcagaa cggaattccc aaagtgcagt cggccagaat cggagcccaa
    19081 taagtattgt aaacgcctat ctttttgaag atgataaaat taggaatcag agtaaatacc
    19141 gacggcagca tctgggtgac caaaaagatg aatatgatcg cccgtttaaa tttaaaatta
    19201 aatcttgcca ctccatatgc ggcgggaatg gataaaaccg tagcgatcac cgttgttgcg
    19261 acagcaatga tcatactgtt tttgagcgaa gctaaaatgc tgaacgattc gttgaacagc
    19321 tgcgtgatat aggggtccag cgtgggatga cgagggaaaa ttaccggagg tgttttaaaa
    19381 atatccgtat tcagcttcag ggagcaggaa aacatccaga aaagcggaag catcatgatc
    19441 acaaacagga agattacaat aatcagcccg ataatttccg gggtccttct tactttctta
    19501 atatttttca tattcacatc gcctcatcag acttaatgag ccttgtatag atcaacccta
    19561 ccacaagcaa aatgacaaac agcacattcg caattgcagc gccctgactg aaattaaact
    19621 cgacaaaaga ccgtttataa gagtaggtag acaaaacctc cgtcgaattt accgggccgc
    19681 cgtttgtcag aacgaagatc aaatcaaata cccggaaggt atagacaaat ccaagaacca
    19741 aaaccgcttc aatggtcgtt ctcatcagcg gcagggtaat atagaagaat ttctgaaata
    19801 ccgaggctcc gtccaaaaca gcgctttcgt aaatatccgt gggaatcgag gtaagccctg
    19861 ccgaaaggat catcatattg aacgggattc cgcaccagat gttggtgatg atggttgccc
    19921 acatggccga agtgggctta aggagccacg gaatcgcttt cggaatcaat ccaagggata
    19981 acagggtttg gttgataact ccgcagtttg tgtcgaacat gaacttgaac aggatactgg
    20041 taactgttac cggcagtatg taggtaatca tcagcagccc tctcataggc ttcaggcagc
    20101 ggaacttccc ggtaaacagc agcgcaaaga ggaatccgag aacaaattga aataagatac
    20161 tcccgacggt gtaataaaac gtattgagaa ttgaagtttt catcagcggc tcggaaaaga
    20221 ctttcatgta attctgaaat cccacaaacg gcttatctcc actgattaaa ttaaacatgt
    20281 tgacgtcttt aaagctgagc gcgatattgt aaaaaatcgg gtatatcatt aaacaacaca
    20341 tatagatcaa ggccggcaaa acaaagagat aaccgattct ctcgtatctt tttttcaaaa
    20401 ccatcacccg attgttttag attttgccag ataaattgtc cgctttttta ataaagctgc
    20461 ccaggcccga accgagcagc tttattctta ttccgtaacg cgcgattact tgcggtttgc
    20521 cacagcctga tcaatcagcg cctgaccgtc cttgactgcc tgttccggag acttttgcag
    20581 ggaaagcgcc tcctgaaggg ccagctggac tccgcttgat atttgcggat aggctgtgtc
    20641 taccggtctt gacctggtcg tggtcttaaa gatatcttta aacatcttcc agttctcagt
    20701 ttctgaaaac cgtgaatctt caatggagtc cgatctttcc gggaactggc tggttgactt
    20761 ggtccattga acaacgtttt ccgttttcag cacatatttc ataaaatcaa aactggcttc
    20821 aacattcttg cctttgacaa tagccagatt ttctcctccc agaacgtcta cggagtattt
    20881 atccttcgga atctggaagc atccccattc aaatttgcaa tcggttttca gattatccag
    20941 atcccaggag ccttcctcca tcatcgcggt tttaccggat tcaaactggc tgagcagctc
    21001 cgactgcgac cagttgatga tatccgttgg cattgagccg tctttcacca tgttgtaaag
    21061 gaatgtcagc gcgcggagcg aatcagggct cgtcatatga tattcgcttc ctccggccga
    21121 ccacagccag gggtcaaact gggaagttcc ctcctcgttt ttagctgcgc acataataaa
    21181 accggctacg ccggattttg tcagtttctt tgcgtctgcc cggagttcat cccaggtttt
    21241 tggagtcgaa gtgatccccg catcgcggaa catttgctta ttgtacataa tgcccagaca
    21301 attgccgtca aaaggaattc cgtaaatttt tcctttatac tgtccggtct ttttagccca
    21361 gtcataatat ttttcataat cgggccaggt ttttacaaag cttgtcagat ctgaaaacat
    21421 tcccattgcc gaatatgccg tattgtcaca attatcaatc atgacgacat ccggaagact
    21481 ggatgcggcg gcgctgatgg aaagctgctt tttaaaatcc gcaaacgcaa gggaaactgc
    21541 tttcacgctt gcaacttttg actgtttgtt atattgatcg cagaaattct gaatcgtatt
    21601 ttttgatttt gtgtcattgt aataatgcca catttgcagg ttaacttttt cggtagtctt
    21661 ttttgtcgtt gtgctgctgc cggcgcagcc tgagacggaa attacaagaa gcatgcttaa
    21721 aaagatcgtc agaagttttt tcatatgatt aacccctctt ttcaaaacaa agtattttct
    21781 tttcgtctga tccgaagctg tctttgcctg gtagttaccg cgattaattt ttcccccggc
    21841 ttcaaactcc cttccataaa atttttcatg ttcgccaact tctaacgcac cgccgtccac
    21901 cccttttctt tttggctgat catgaataag ccttgagtaa atcgggtttt ccttttagaa
    21961 actttggcag aaccgagtta ctgccgcccg acgtttccga tcatttttac tgcttgttag
    22021 tctttcaatc catctctccc gtggtcttga actgctgcaa ttcaataagt aaaaccattc
    22081 tctgctattc ttgccgcgca tctttttttg gttatgaaaa agatcaaaaa agatgtcgcg
    22141 gcgttttcta tttaagtgct gaaaagagca aacggtgcgc gaaaatcgcg cagctctatg
    22201 ggagcaattg tcggaaagta tctgtttctc ttgatggttt gccggccggt aaaaaactct
    22261 gatttcggca gaagtttcat tatttaccgg agtgggtcgg gtaatggtct tgtatgataa
    22321 atcgtttcct aataacattt tatagaaaac ccattttttt attcctgaat tttattcaga
    22381 cctcatcaga taaacaaatc caacttttcc tgccaaaaaa tttgtttgag gaatttataa
    22441 ataatttaga attacagcgc gctatttttc ggttctacaa gctttttttg ataaaacgta
    22501 cagtaaatta cacattaatt gttacaataa gagtcaatcg gtaaaaattc catttttacc
    22561 gaaaaaatag aaaagccccg ccgtaataat aaactataaa tcgtcaatca tacggagagg
    22621 atttttctaa tcagaaagca ataaagatta tgtgattatc atccatctaa taagaaagaa
    22681 gcaacttgat tttggttaaa atactttatt tgaacggtaa aattaaacgg agcattgaat
    22741 tttaataaaa ttcaatgctc ccctgcttta tttggtgatt taatatcata ttgttttata
    22801 tttatttcgt ctaaagaatg aaataatagg aatcatgtag atttttctgt tcgtattttt
    22861 ctgaaagcct gtgaagcaag ttttttaccg gttttattcc agacctgttg tgcctgaaat
    22921 catccaatct tcgaaaaaaa ttcttctttt ccttttcagt ggcctgatat ttaaaatatc
    22981 gccataaatg gagcgccgcc ttttcagcgt tgccgggttt cacctccgtc tcaatcgctc
    23041 tttcaatgat ccgataaaat tccgtcgccg ggtaacggtt tttatccttc agaagttctc
    23101 tttccgccct gtaaccggaa gggtcgtttt caagaacctt atacttatat ttttcccatt
    23161 cttttttgag tacgtcgatt ttcccatcct gagcggtaca gttgatacat ttgactgcgg
    23221 acagattttt atctttgacc tccagcatca tgtcgatccc acggtcaagc tttgaggcaa
    23281 actccaaaaa agggtcgatc cgtatcgtag aagaatgcgc gcccggtctt ttgcccggat
    23341 tctgctgggc ataatgaatt ttttgaggcc catcctgttt tccccaggta tgggcgcagg
    23401 ctctcagcca tttcatcccg tcgccttccg gggcgtcggg atgaacggca tgatgaagat
    23461 tatcgaacac gaccggtatg ccaagttgtt ctccaattcc cagtacgtcg ccgatgtggt
    23521 agcatctctc gtcattttca attgccagtc ggctttgaat ggcctcatcc agccgacaga
    23581 aattatcctc aaacctcttg accgcctggt ttttgtcgcc ataaaccccg ccgacatgca
    23641 gaattatttt attttccggc ccaacaccaa gactgtcaag aacatcggta tgatagcgga
    23701 ggtcggcgaa tgctctgccg accacatccc cgtcgggcga attaatcacc gtatactgcc
    23761 cgggatgcat ggaaacccgc atgccggagg actgaatttt ctctccaatc gacgacaggc
    23821 gtgaagcgta cagtttccgc cattccagcc ggttgaccgg gcttgaccca aacggaatca
    23881 aatcggagct gattcggaac agtccgatcg aattttcaat attataatcg atcatctttt
    23941 caagtgcggc cagattgtct tcaattatac tgctcaagac caaattgtcc gcgctttttt
    24001 ttaagcatct tttgaatccg gttccgtaag tccctatcgt caggcatgca tagccgatcc
    24061 tcatcttttc atctcccctc atgattttca tccttttatt gatcattatt cgaaacagtt
    24121 cgcagattat tctccctttt gccgcataga aaagcattgg ccgcggggca atcacccgcg
    24181 gcacgcatca gtccgggaat cctttctgaa attataatac tcatccacca ccgccggccc
    24241 ttcaagaatg tccctgccga catacatagt tccatcctgt ttggtgcgga cgctccactt
    24301 gatcagctga attttgcctc cggcaatctc gatcccggta atacacctgg gatgaacgca
    24361 gcagccatca ttaaagtaaa gggattctcc aggctgcgga aataccgggc ggtgggtatg
    24421 acccgctatg gttatgatct gataacgctc cgcccattcc tccagccatt tttccacggc
    24481 ttcctttttc tgatgatttt tcgccgtgct cgtagggtct ttaattccta tcatctcaag
    24541 gggcttccat acatgtctta ccaaaaaccg tcccagtttc caccaatcgt cattaaatcc
    24601 gtcggcctga tggccgtgca gaagaaagat ttgtttttgg gacacccgat gtgtcagcac
    24661 aacagactcg ttaaaccgaa ttttcggcat cagcacgatc ttcttttttt gttgctcgtc
    24721 ataatattga taaaagccgc atgacgcgct gctgcttttt tttactttat catgattgcc
    24781 gtaaagcaga taaagccttc cctcttgatc aaatctgtca agctgccaga acgcgtcgct
    24841 gtgaacactc atgatatcgc tgattttttt attcttccaa agctcgtctc cgtcgccgat
    24901 ctcaaggtaa gtataattgt ttttataata atacgtcagc gccgcaaaat aaagattttg
    24961 gtttcctgaa aaatcatcgg cccagctgcc gtccccccgg tggcaatcac tgaacagaat
    25021 aatcttggaa gagtcgtcaa aaccaatccg ccttgcctgg cggtaagccc gggtaatata
    25081 tttgaaccgg ttcataagtc aggccccctg cttaggccgc acagcctgct gagaattgcc
    25141 ggagatcggc cgggcccgtt cgttcaaagg tatttttgaa tggttttaaa aatttcatta
    25201 atttgttttg tctttctaaa gttgaaaatc tcattgagca tggcaggaga ttttctgcgt
    25261 ataagcctat attttgatgg catatcctgc gccgcccggg taatcttttg atattcttcg
    25321 cacatcagcc ggtttttcat ttgaatcaga tggtctgtcg ctggaattct cgttaaaggc
    25381 tgacgggagt gcggcgtttg aaatgtcagg ctgacggtgt tgtcgtgaac agcatattcg
    25441 ttactgtcat aaccggcgtt gttcaggccc ccgataaatg cgtcgcacat ctcgtgattt
    25501 tttaaggtca cgctcaaata cagggtcagc tcagaaggtt ctgaaaattc gccgagttta
    25561 aatccggtca gccaccactg tttctcctgc cgggtaaaaa gagtataacc gtttttcttc
    25621 aggctcatcg taagataaag catattattg tcatccacgc tttgataaaa cgtaccgcta
    25681 aacaggcccg gaatatccag gtcgggaatg tctgtggaaa acacgccaat ttcagcgccg
    25741 catgtgaggt catactggcc cttccagaat tcgatcagcc accgctttga accgtattca
    25801 aaatataccg gatcgctgtc gagaatcatg ccggatgggg ccgctgcctc gtcataaagg
    25861 cggcagtagc cgaattccct ctgccaggcg tctttgatgg agtaaaaaat atcctgctga
    25921 gggtcgtatg taaaacccgt atcctccagc gcttcctcca gcgcccgact gttaacagcc
    25981 tttcttctga acagccatat cagcagtctt ttgagtccat ccactttccg tattaaccac
    26041 cgaggcttca gagcaaaagc cagtaatacc gccgcaaatg caaccgtaat taaaagaacc
    26101 gccgcgacca gcaccgcgtt ctccgtatta ggggaagccg ctcccacaaa aaggggcatg
    26161 atcattcgga tattcattat cataatttct gctccacatc tgatttctta caacatcata
    26221 ttcgcggaac aaatagaatg ttaaccctgc gcgggaccgt tcgtaacgaa tgaccctcgg
    26281 cattatccaa agaaaaaagc cggggcttag gccccagcga caccgcatta tggcaaaacg
    26341 tttgcatttt tgaaatgaag ttctataata aacttaaaaa cggcagtgag ccaacgggag
    26401 ttttgatcat gaatctgacc gatgaactga atcagaagaa aatagaagaa cggtattcgc
    26461 ttgcgctcag gcaaacacag tctctgctgg aagatgagca tcatgtgatt gccaatctcg
    26521 ccaatgcctc ggcactgatt tacgagacaa tgagcgatat taactgggcg ggattttatc
    26581 tgaaaagcga cggggaactg ctcctcgggc cttttcaggg taaagttgcc tgtgtgcata
    26641 ttgccctggg caaaggcgta tgcggaacgg cggccgccac aaacagaacg attatcgtcg
    26701 aagacgtcca tcaatttcaa ggccatatcg cctgcgactg ctcatcccga tcggaaattg
    26761 tcattccgct cagacaagac ggagccgtcg tcggagtttt ggatattgac agcccttccg
    26821 tcggccgttt taatcaggct gacgcccgct ggcttgagcg gatcgcccaa accatagatg
    26881 aatcctgcga ctggaattcg acgttttcgt tctgatcccc gggcttaaga cggctcactg
    26941 atccgtattc cggaatccct ttccgataat ttcggacgtt gtcgtaatga caataaacgc
    27001 gcggggatcc gtttggtgga tataccccga gagcttgatc gcctgacttc ggctaagaac
    27061 aacagtaagc agttgtttct catcatgcgt atagcctccc tgagcgggaa taatcgtaat
    27121 cccccggtgc atctcctgaa ggatatagtt ttcgattttt tgcggctgtg acgtaatgat
    27181 ctccagtttc ttgctcgtat agagaccctc aagcaccatg tcgataataa accctttcat
    27241 aaccagcccc aaaacagagt acatcccctg ccggacgccg agtgtgaaaa gcgcagaaaa
    27301 cgcaatcaaa aaatcagcca gcagcagggt tttacccaca tgaagatggg tataacgggt
    27361 caggattttt gccacgatat ccgttccgcc tgtggaagcg ttctgactaa agacaaacgc
    27421 ggaaccgatg gcgggcagcg tgatggcaaa gatcagttca agcatcgaat ccccggtaat
    27481 cggttttgtg aggggaatca atttcgcacc gacccacaca aagaaggaaa gcgcaaaact
    27541 cgaataaatg gtctttatcc caaaatcttt tccgagcatc agaatcccca gaacatccag
    27601 cacgatattg atcagcagca tgattagtcc aacattttgt cccggcatgt atttattgat
    27661 aatgatggcg ataccgctga ctccgcctgt tgcaaatcca ttgggtgttt taaacaaaac
    27721 gatacccatg gcggttaaca ccagacccat attgacaaac aggaattctt tcagctttga
    27781 gcctttcatt tccgttcccc caagacattt ttgtttcaaa gacgccattt gccattatac
    27841 cctttcggct tttggttata caacgggcat atgggcccat atgaggcctg atgaacggct
    27901 caggccgtac gttgatttat taacgcgggt atccctttga atccttgctt ttcctccctg
    27961 ttttttcagc tttatccgat aaaaaccatt gaaaaaagcc cttgactctg acgctgcgtc
    28021 atggattata ctgtttgcag gtgataagaa tgaaaacggt cagtcaggtt tcaaaacttg
    28081 ccggaatcag tgtgcgcaca ctgcattatt acgatcagat tgggcttctc agccccgaga
    28141 cggcaaaatc ggggtatcgg ctttacagcg acgccgatct tgaaaagctg tggcggattt
    28201 tattttaccg ggaactggaa tttccgctgg aacagatccg gaaaattctg tcccatccat
    28261 cctataaccg ggacgaagcc ctccgcggac agcgcctgct gctcgttgaa aagaagcggc
    28321 gtcttgaaaa tcttattgcc tccattgatc aaacattgaa aaagggattt gaggtgaaac
    28381 tgttgaaaac ctttgataaa atcagcctgg aagaacacaa aaaaatatac gccagagaag
    28441 cccgggaaaa atacgggaag atttatgagc aaagcgaaga aaaaacttcg caatactcgg
    28501 aagaccgctg gaacctcaac atgaagcagg cccagtcgat tttcgacggc ctcgcggcat
    28561 gtatggaaca gagccccgac agcccggaag tccagacgct gattggaaaa tggcgcgctc
    28621 atatcacgga aaattattat gaatgcacgc ctgaaatatt ggaaggtctc ggaaagcttt
    28681 atctggaaga tgaacgcttt acccggaata ttaacaaaac gagacccggt ctcgcggaat
    28741 ttctcagccg cgcgattgga gtttactgcc gaaaacttcg gggataggag taagcccgaa
    28801 atttctttca gactgaagag aggcatcggc tttcgccgat gtccctcttt tttttaacag
    28861 ttattcgcgt ttctacccac aaattttgaa ggccgctcat tccggttgtt ttgattgggt
    28921 caggcggatt ttgtacactc tccggctctc agtcccagtt tttcaacagc ctgttccctc
    28981 attttaaatt tcaggatttt tccggccgcg ttcatgggaa attcggtgac aaactcaatg
    29041 taacgggggg ttttatgccg ggccatgtga gaacggatat ggtcctgaag ctcggcctcg
    29101 gtcatctcct cgccttccct caggattaca caggccatga tttcttcccc atactgctga
    29161 tccggcacac cgatcacctg cacatctttg accttgggat gatgatagat aaattcctcg
    29221 atttccttgg ggtagatatt ttcccctccg cggataatca tatcgcggat tctcccggta
    29281 atcctgaaat tcccatccgg gtctctgcgt gcgagatccc cggtatgaag ccacccgtcc
    29341 gggtcgacag ccgccgccgt tgcttcgggc attttatagt agcccttcat gacattatag
    29401 ccgcgggcga caaattcccc atcctctccg tcgggcaggt ccttccctgt tttcgggtcg
    29461 acaattttgc actccactcc cggcaaagca cggccgacgg tattgacccg aacttcgagc
    29521 gggtcgtcaa cccggctctg agtacatccg ggagcccctt cggtctggcc gtaaacaatc
    29581 gagatttggg tcatattcat tttttccact acgtcgcgca tgacctttac cgggcagggg
    29641 ctccctgcca taattccggt cctcatatag gagaaatccg tcttcgggaa atcttcatgt
    29701 tccagcatgg caatgaacat ggtggggacc ccatgaaaac aggtaatctt ttctctgtta
    29761 atgcagtcga gcgacttcct cggtgaaaaa gacggaagcg gggaaagcgt aacgccatgc
    29821 gtcatggccg ctgtcataga aagcaccatc ccaaagcagt ggaacatggg aacctgaatc
    29881 atcatccggt cggcagtcga aagatccatg cagtccccga tgcatttccc gttattcacc
    29941 acattataat gcgtcagcat cacgcctttg ggaaacccgg ttgtgccgga ggtatactgc
    30001 atattacaga catcatcctt attcacggct gcggttcggc gggatacctc ggcaagcgga
    30061 acctgctccg ccatttgtat ggccccttcc cagctgatgc atccctttcg gctgaatcca
    30121 accgttacaa cgttgcgcag gaacgggagg cgttcgctgt gaagcggctc tcccggcgcc
    30181 gtatcatcaa gctcggggca gagttcctct atgattccgg cataatccga atccttaaac
    30241 ccctcgatca tcaccagcgt tttcgtatcc gactgacgca gcagatattc cgcttcatgt
    30301 attttataag cagtgttgac agtgacaaga atcgccccga ttttcgtcac tgcccaaaaa
    30361 gtgatatacc attgcggaat attggtggcc catatcgcga catgatcgcc cggctttacc
    30421 ccaagcgcaa ttagcgagcg ggcaaaggta tcgacgtcgt cgcggaattc cgaataggtg
    30481 cgggtataat ccagctgcgt atagcgaaac gcatactggt cggggaactc ctccgtcatc
    30541 cggtcaagca cctcggaaaa cgtcaggtca atcagcgtat ccttctgcca aacatactta
    30601 cctgtttttt ccatattgtc aaatagcctt tcgctgtatg tacctgtttt aagggagccc
    30661 tttttatctt tcagatttag ggagaaacaa cttccttcat tctgtttgca tttcttggtt
    30721 gaaaacatag tttccatcgt attttcctcc tgaatccgcc gtatcccggc gtcatttttc
    30781 tccaaaagag taaaagaaaa cgctttcgtc tccagcattt cctgctggag gcgaaagcgt
    30841 atgcttccgt ggtaccactc cggttggtat aaattatacc ctcttaataa catcaatcca
    30901 tcggatgaat gttctctcat ggtaacggtg agaatcccgg tccaacctaa tattgcgccc
    30961 tcgaacactg gcacaacttt cagctgactg ctcaagggcg agttcgcatc cgctctgagc
    31021 actgttttgc atcgaccaac agctctctga agcttcacaa acggagcttt ttccctatca
    31081 tcgcatttga ttattattta gattttagca aagaaattgc tttttgtcaa gagctatctg
    31141 ggaacctgct gcgtgataca gtgaataatt cctccgccaa gaagaatctc acgcgcgaaa
    31201 accccgacca cctgacggtc cggataaatt tcactcagag tttgaatggc cttcccgtcg
    31261 ttcgggtcgc caaactgcgg aacaataatt cctccgttgg ccgaatagta attaatgtag
    31321 gaagccggca gcagatcgcc ctcctttctg ggcgcggttt tcactgccgt gtcgacgccc
    31381 cggctttcat cagcggtgat cctgaccgcg gattcaggca gctccagcct gtaaatcttc
    31441 agtttcctgc cacgcgcgtc ggttgcctta gaaagaattt cataggcctc gcggcagatc
    31501 tcataataag gattgctctt tttctccgtc caagccgtca ccacgacgcc gggccgggca
    31561 aaacagcaga caagatcaat atgcccgttg gtctcattcg gatcaagacc ccgttggatc
    31621 cagatcacct tttctacccc cagatagtcc gaaagtttcc gttcaatttg agcgcggtta
    31681 agctgcgggt ttcttcctgg actgagcagg cactcctccg ttgtcagaag cgtaccttct
    31741 ccatcgcagt gaaacgagcc gccctccagg acaaatccat ccgcctgata aacgtctttt
    31801 tcttcaattt cacagacttt ttctgcgatc tggctgtctt tgtcccacgg aaaatacagg
    31861 ccgtcataga ggcctcccca tgcgttaaat tcgaaattga cgccccgaat atcatctttt
    31921 ttatttttga caaagaccgg gccgcagtca cgcatccagc aatcgttgct ggacatctct
    31981 acaattttaa cctgaggcgg caactccgag cggacatgtt catactgccc ggcgccaacc
    32041 gcaacacgaa ctttttcaaa acgactaatc gctgcaataa cctgtttaaa agcctcctgg
    32101 gccggttttg cgcccagcct ccagttatcc tgtctttccg gccagattag ccagcagccg
    32161 tcgtggggct caaattctcc cggcatccgg taaccatccc gtttaggtgt cgaatctgtg
    32221 atgattttca taatcggtct cctttatcat ctttccggtc tattctcatc ctaccctcaa
    32281 cgcataagaa aaacaattac ccattgtaat gactccgggt atctactaca atagtagtag
    32341 atgatttatc ctattacgaa atctgaacgg agtatccaca tggaacgtct ttctcatgaa
    32401 gaatggtcgg caatcaacgg tctttgctta tccatccatc tttccaccga cacgcacagt
    32461 ctccgcacca ctgtactcaa cggcctgaaa aagctgattc cctatgacag ggcatttttt
    32521 gatctcgcct attacgcgga cggcgacgtg atcttttttg accccgtttt tataaatatg
    32581 agtcaaaaaa gagtacattt gtactttaag tattttcaaa tggtcgatta cgcccgcctg
    32641 atttttattg agaataaatc agcggtctat cgggatacca gtctgctgcc cgacagtctc
    32701 agggaggaat ccattctttt taaaaaatgg atgcagccaa tgggcatgtt tttcgcaggc
    32761 ggtttcagcg taatggaaaa aaagattctt tacggttcgg ctaatttgtt ccgatgccgg
    32821 gaacatgggg attttaccgc ccacgagctg gaaatccttc gtattctcaa cacccacatt
    32881 tccaaaagat ttcactacct gtttccgagc ggcctgaccc aatcggcgga ggcaactccc
    32941 gaaagcataa attttcacgc cctgaccagc cgggaaatgg aagtgatgga atgcatctgt
    33001 gagggactgc tcaataaaga aattgcagac aagcttaaga tatcggagtt taccataaaa
    33061 aaacacatcg gaaatatttt tcggaagatg aacgtcacaa accggtacca gctcatgagc
    33121 aaaatctatc acacggaaca ggacgaccgg cagcaggaag caacgaacct aaacaaaaaa
    33181 ccgtcacgct tgtgacggtt tttttgcatc ctttttttaa acagatcagc gacaatggtt
    33241 ttcacagctg cagttgtgat cgtgttcatg ctccccatgg ctgcagctgc cgcccaggtc
    33301 tttcagcgtt ccgtcaagat aagcttttac cgcgtcgtcc acgccgcctt ccacgccgga
    33361 tacgagttca ataccgacag aagatagcat ttgttttgct ccaccgccaa tgccgccgca
    33421 tatgaccaca tcgaccccgg cgtttttcaa gaactgcgcc agcgcggaat gtccgttttg
    33481 gcttgaatcc atcttcgtct tgccctgaat ttttccgtct ttgacgtcaa aaagggagaa
    33541 ctccgggcat ttgccgaaat gctgaaaaat ttcgctttcc tgtgttgtaa cagcaatttt
    33601 catcgtgact gaccctccat aaattcttcc atgattccag cggcgctttt aacaagctcc
    33661 acacatggac gtttttggta atagtcttcg gtcctttttt ccggaacagg gttcccctcc
    33721 ggtttttcaa gcccaagcag ttccctgcag atgatcgaac cgttttgctc ctgaaacttt
    33781 tgcgcaagct gctgaatcag cttataatgc tccgctttca tctctcctgc attcggcagg
    33841 ctatatccgt atttcaggcc cgcaatcata aacatggccg ataccgctcc gcatacctcg
    33901 cgcagtcttc ccattccacc cccgaacgaa gaagacaatt tgaatgccgt ttccctatcc
    33961 atgccgacat cctgcgcaaa tgcgccaaag actgcctgac tgcaattata gccctgtaaa
    34021 aacagctttt ccgtttcttt tattttactg tccatccctg tcactccatt tctgaaaggg
    34081 gcgcgcccgg caccccctta ttctcaaacc ggcaactgcg gcaggccctt cggcagccgc
    34141 agcgatgttc ggccaactca tagtttcccc cgccgatttt gatgtttttg cccgagcaaa
    34201 gcgcttcggc aacttttttc cgggcctgat aaagcgtcct ttgaaaggtt gcccgggaaa
    34261 cattcatcct caccgaggct tcatcctgtt ccaggccctc aaggtcacaa agtctcagcg
    34321 tttcaagctc ctcgaccgta agcgctaccg tcgcggcgca aggtctttga ggcgtaaacg
    34381 cccggttttc cggaacggcg cacactctgc ggcatttaaa acctctcggc attgatccac
    34441 cctcccaaga ataatgagca tatgcacaat aaaactatta tagtgtgcat atgctcattt
    34501 gtcaataaaa tcatctgttt ttaatcctta acaccgggaa actcccgaca ccccttgaaa
    34561 ccgaaacttg atccgttccc ccgcttacgc gcagacttga ttcatgcctt tatattgtta
    34621 aaagctgaaa cgccgggcat gaaaacccgg cgtttcttag gaattccgca aaaggtgctg
    34681 tcacggtcaa gcagactctc caccgtatcc aacccaagcc cggcgaaaga tctccaatac
    34741 cggaggattg tcgattggtg tcttactcgc agggctgttt actgaacgga tatatttaat
    34801 ggccggagag attgtgaaat cacctgtttg atcttctggc aggcctcttt cgttttgcct
    34861 tcgcagcgaa cgatgagttc cggccctgtg ttggaagccc tgacaagccc ccagccatca
    34921 ttgaaacgtg cccggactcc gtccacttca attgtatcga gggcgaactt gcggagttct
    34981 acgcgcgctt tttcaacata ggcatttttc tcttcctctt tacacggcac gcgaatttcc
    35041 ggggttgcaa atgattttgg caggccggaa agcagctgat gaagcgaatc tccggtattt
    35101 gaaagaatac gaagtagcct tgccgccgcg taaacggcat catcatatcc aaaatactcg
    35161 tccgcgaaaa acatatgtcc agacatctca cccgtaaaaa cagcattgat ttccttcatt
    35221 ttcgctttaa tcagagaatg tcccgtttta taaaacatcg ggttgccgcc aagctttttg
    35281 atctcgtcca ccagctggtc ggaacatttg acttccacaa tagcgtccgc gcccgggtgc
    35341 ttcggcaata tttcccgcca gtacaaaacc atgatccggt cgccccagat gatctcacct
    35401 gtctcatcca ccacgccaag ccggtcgccg tcgccgtcaa agccaagccc gacatccgct
    35461 ttgttttccc tgaccgaacg gatcaaatcc cgcatgttgt ccacgcgcgt cgggtcggga
    35521 aaatgatgtg gaaacgaggg gtccgaatca cagtaaagcg ggatgacctc acatccgatt
    35581 tcttcaagaa tctttattgc aaactctcca gccgttccat taccggcatc ggctacaacc
    35641 tttaatttcc tgtttccaag tttgattttt cccatcatca tacggaaata atcctcaaca
    35701 ggagaacaat tttcgtatcc tccctcgcca tgctcaaacg agcccttcaa cgcgatttct
    35761 ttgatttcct gcaattccgg gccaaaaagg gttctgcccg cgtactgaat cttaaacccg
    35821 ttatattggg ccggattatg gctcgctgtt accatgatcc ccgaactgat gtgaaaatca
    35881 atcgtggaat aataaaaaat aggggtgatt accgtaccga tccctacaac atttatgccc
    35941 gtagaccgga ggcccttaat gatggactgc cgcagcacgg gagaagactt gcggttatcc
    36001 atgccgacaa cggcttttgt ctcacccttg cgtttgatat atgtaccaaa agcctttccg
    36061 atccattcgg ccttttcatc cgttaaatca tcgccataaa ctccccgtac gtcatactgt
    36121 ctgaaaatcg tgtcaaagtt caatctgatc acctctcctt ttgaaatccg gaattgcctt
    36181 ttcccacgcg agccttgcaa tcagccatga caccaaactt tccgctccct cattacaatt
    36241 cgggccgatt ggcgttatac catccataca gccgcccgta tcagggtcaa tcagagaaat
    36301 ctgcgctgaa ttctcacctg tataccagcc aagacaccgc tttgcccagc gaatatactt
    36361 ctcgtccttc gaaagctcga atgctttcag gcagacaagg agcatttcac aggcctcaac
    36421 gggctgttga tcaaatggag ccgccatctc tcccttttta agccagccat tacagccaat
    36481 cggatgaaaa tgattttgac ggaaagtcgt ctgtgaaaga aaatccagac tttcaagtcc
    36541 tatttccaga taacgtttct cctgcaaaac ttcataggca ctcagcatcg cccacggaag
    36601 cacggagttg caataagtca tccggctttc aaaccaatgc cacgtttcct cgtcattgtt
    36661 ctcataag
//
LOCUS       DATALY010000097         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_21336,
            whole genome shotgun sequence.
ACCESSION   DATALY010000097 DATALY010000000
VERSION     DATALY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21336"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VHR42_02965"
     CDS             <1..771
                     /locus_tag="VHR42_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3026175.1"
                     /translation="AFAGIITGFYMEDGTIQMLIAHQSPFLIVFGGTIGIALVAFPLK
                     NLKAIPGALKLIVMPKKHDYAGLVDMLCDIANKARKDGLLSLEAEADKISDQFIKRGL
                     GYIADGVDPEFLKKVLYNEIESEYRKYDDAAKVFINMGGTAPTMGVLGTVMSMVSILA
                     KGADDSNALVKNISTAFLATFFGIGSANILWLPLGNQIKVVAEQQSDYREVVVEGLLA
                     IQSGEPSTRLRDRLFAKIGEIKSGKSKANEKNSEVNEE"
     gene            828..1490
                     /locus_tag="VHR42_02970"
                     /pseudo
     CDS             828..1490
                     /locus_tag="VHR42_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
     gene            complement(1442..1744)
                     /locus_tag="VHR42_02975"
     CDS             complement(1442..1744)
                     /locus_tag="VHR42_02975"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX3026176.1"
                     /translation="MKKEPETDNRERYLLTYGDLMNLLLILFIVLFCVSKRDVVRTHD
                     DSILPPAASCIVLTISPEVTDLSDGVMAFLSIVITTRRFFALFSGVSFFATGKYSP"
BASE COUNT          582 a          440 c          466 g          472 t
ORIGIN      
        1 gcttttgccg gcataataac cggcttctat atggaggacg gcactatcca gatgcttata
       61 gcgcatcaat cgccgttcct aatagttttc ggcggtacga taggcattgc gcttgtagct
      121 tttccattaa agaatctaaa ggcgattccc ggggcactga aactgattgt aatgccgaaa
      181 aaacatgatt atgcagggct tgtcgatatg ctctgcgaca ttgccaacaa agcgagaaaa
      241 gacgggcttc tttcccttga agctgaggcg gacaaaatat cggatcagtt tatcaagcgc
      301 gggcttggct acatagcaga cggcgtcgac ccggaattcc ttaaaaaagt cttatataac
      361 gagatagaat ctgaatacag aaaatatgac gatgcggcaa aggtttttat aaacatgggc
      421 ggcacggccc ctacaatggg tgttttaggt acggttatgt cgatggtttc cattctggca
      481 aaaggcgcgg acgattcaaa cgcccttgtt aaaaatattt cgacggcgtt cctcgccaca
      541 ttctttggca tcggttccgc caatatcctg tggctgcctt tgggcaatca aatcaaggtc
      601 gttgccgaac agcagagcga ttaccgcgaa gtcgtggtgg agggtctttt ggctatccag
      661 tccggagagc cttctacaag gctgagagac aggctgtttg caaaaatagg cgaaatcaag
      721 agcggaaaat caaaagcaaa tgaaaaaaac agcgaggtaa acgaggaatg acagaccaaa
      781 ttgagccgaa tttgcaaatt gaaaccgtca attaaggggg cgccgatatg aaaaaagagc
      841 ctgaaaaaga taatggtgaa agatacctgc tcacatacgg cgacttgatg aaccttttgc
      901 tgatattgtt tattgttctt ttttgcgcgt ccaagcagga tgttgtaaaa acgtcaaaaa
      961 taatgcagag tatcaaaaac ggatttgcag gcggaggcgc gtcccagtat accgctgcgg
     1021 cgaccggctc ttccggcaaa aaaggcaatg ccgctaaaaa tgtcaagagt gttaaaggcg
     1081 caacagatta cagcgatttt tacgaacagc ttatcaatgt tatgaaacaa aaaggtgtac
     1141 tcgacaaagt caacataacg gcatcaagcg aagaagttat tatttcactc aaagataatg
     1201 tgctgtttgc ccagagcaag gccgtcgtaa gccctgacgc gacaagtctg ctcacaacaa
     1261 tcggcggcct tatgacaaaa atctcatacg gacagcttat aatagaagga cacactgact
     1321 ctgaccctat ccatacggaa cagtttaatg ataaccgcga gctttcgctt atgcgcgcat
     1381 ataatgtcgc gcagatattt cagggctgcg gtcttgaccc caaaaaattg ctgcctgtcg
     1441 gctacggcga gtattttccc gttgcaaaaa acgatacgcc ggaaaacaag gcgaaaaacc
     1501 gccgcgtcgt tatcacgata ctgagaaaag ccattacgcc gtccgacaaa tccgttactt
     1561 ccggggaaat cgtcaagacg atgcaggacg ctgcgggcgg caagatcgaa tcatcgtgtg
     1621 tccggacaac atcccgcttt gacacgcaaa aaagaacaat aaacaatata agcaaaaggt
     1681 tcatcaagtc gccgtatgtg agcaggtatc tttcgcgatt atctgtttcg ggttcttttt
     1741 tcatgtgtgt gccctcctga attgacaatc ataaaatgaa acgggctcat tcaattcggt
     1801 ttttccttat tgcgcatgaa aattccttgc cgatcagcgt taaaaaccaa caagtcgctt
     1861 gaattttaga aaatcagctg ccggacgacg atgattcgat cttgccgccc gcagcgtcct
     1921 gcatcgtctt gacgatttcc ccggaagtaa cggatttgtc
//
LOCUS       DATALY010000098         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_367825,
            whole genome shotgun sequence.
ACCESSION   DATALY010000098 DATALY010000000
VERSION     DATALY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367825"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VHR42_02980"
     CDS             <1..165
                     /locus_tag="VHR42_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007431677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX3026177.1"
                     /translation="GRAEKKQVMEMTRMLLGLAKVPKPDDTADALAMAICHAHSSGSL
                     MSGILNQIAR"
     gene            187..780
                     /gene="ruvA"
                     /locus_tag="VHR42_02985"
     CDS             187..780
                     /gene="ruvA"
                     /locus_tag="VHR42_02985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX3026178.1"
                     /translation="MIYSVSGILTHIEPGFAVIECGGVGFKCLTTSNTIGKLPKTGEK
                     TRLYTYLNVREDALDLFGFADDRELQCFKMLITVSGVGPKAALSVLSEMTPEKFALYV
                     ASSDSKALQRAQGIGAKTAQRIVLELKDKVSGEQAALGFKQEELPVSASGNIEEAISA
                     LMVLGYSRSEAASAAAKSDSSLSVEEIIKQSLRLLAK"
     gene            799..1830
                     /gene="ruvB"
                     /locus_tag="VHR42_02990"
     CDS             799..1830
                     /gene="ruvB"
                     /locus_tag="VHR42_02990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX3026179.1"
                     /translation="METDFENRIVTPVATPEEMDAETSLRPHSLDEYIGQQKAKENLK
                     VFIEAAKKRGEALDHVLLYGPPGLGKTTLSGVIANELSVNMRVTSGPAIEKQGDLAAL
                     LTNLAPHDILFIDEIHRLSHSVEEVLYPAMEDYALDIIIGKGPSARSIRLDLPKFTLI
                     GATTRAGQLSSPLRDRFGVILRLELYTVEELSSIIMRSAGILDIKCEKEGAGELARRS
                     RGTPRIANRLLKRVRDFAQVCGDGIVTRQVAKQALDRLEIDPLGLDNIDRRILTTMIR
                     NYNGGPVGLETIAASIGEESVTIEDVYEPYLMQIGFLSRTPRGRCVTPAAYKHLGLDA
                     PDNGQQSLL"
     gene            1907..>2833
                     /locus_tag="VHR42_02995"
     CDS             1907..>2833
                     /locus_tag="VHR42_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX3026180.1"
                     /translation="MGRLFGTDGVRGIANTELSCELAMQLGRAAAMVLTEETHHRPQI
                     IIGKDTRISSDMLESAMAAGICSVGANALVLGVVPTPAVAFLVKKLGADAGVMISASH
                     NPAQYNGIKIFSGSGYKLSDSLEEKIEAIVLDGAEKPPIKAGDGVGRVLKPKRAVDEY
                     IKYLKSTTETKFTGLRIAVDCANGSASVTAPLLFEEMGGEYEILSAKPDGLNINNACG
                     STHIEMLSRFVKDNGCDIGVAFDGDADRCLAVDEKGELIDGDKLIAIFSASLKKRGKL
                     KSNTAVVTVMSNLGFFKFAEKHGIDAARTKVGD"
BASE COUNT          776 a          667 c          784 g          606 t
ORIGIN      
        1 ggcagggcgg agaaaaaaca ggtcatggaa atgacgcgta tgctgctcgg acttgccaaa
       61 gtcccgaaac cggacgacac cgccgacgcg ctcgcaatgg ctatctgcca cgctcattct
      121 tccggctcac tgatgtcggg aattttaaat cagattgcac gataacaata agggaatgaa
      181 taaaaaatga tttacagcgt aagcggcata ttaacgcata tcgaaccggg atttgcggtt
      241 atagaatgcg gcggtgtcgg atttaaatgc ctgacaacgt caaacacaat cgggaaactt
      301 ccgaaaaccg gcgaaaaaac gcgtctttat acatatctta atgtaaggga agatgctctc
      361 gaccttttcg gttttgccga cgacagggaa ctgcagtgct tcaaaatgct cataaccgtg
      421 tccggtgtcg ggccaaaggc ggcactttcg gtactgtcgg aaatgacccc cgaaaagttc
      481 gcgctttatg ttgcatcaag cgattccaaa gctttgcaga gagcgcaggg aatcggggca
      541 aagaccgcac agcgcatcgt tcttgaactg aaagacaaag tgagcggtga acaggccgct
      601 ctcggattta aacaggagga gcttccggtt tcggcgtcgg gaaatattga agaagcaatc
      661 agcgcgctga tggttctggg ttatagcagg agcgaagcgg cttctgcggc ggcaaagtcc
      721 gattcctcac tgtctgttga agaaataata aagcagtcgc ttcgtctgct tgcgaaatag
      781 aattaatggt gaatttgaat ggaaacagat tttgaaaacc gcattgtaac gccggttgca
      841 acgcccgaag aaatggacgc ggaaacttca ctgcgccctc attccctgga cgaatatatc
      901 ggccagcaaa aggcaaagga aaacctgaag gttttcattg aggctgcaaa gaaacgcggc
      961 gaggccctcg accacgtttt gctttacggc ccgccgggac tcggcaagac tacgctttcc
     1021 ggagtaatcg caaatgagtt atctgtcaat atgcgtgtaa cttccgggcc ggcaatcgag
     1081 aagcagggcg accttgcggc actgctgaca aaccttgcgc cgcatgacat tctctttatt
     1141 gacgagatac atcggctgtc ccacagcgtt gaagaggttc tttacccggc gatggaggat
     1201 tacgcgcttg atataattat agggaaaggg ccgtctgcgc gttcgataag gcttgacctg
     1261 ccgaaattta cacttatagg cgcgacgacg agagccgggc agctgtcctc gcctttgcgc
     1321 gaccgcttcg gcgtaatact gcggctcgag ctttacaccg ttgaggaact ttccagcatc
     1381 attatgagaa gcgcgggaat cctcgatata aaatgcgaaa aggaaggcgc cggggaactc
     1441 gcaaggcgct cccgcggtac gccgagaatc gcgaacaggc tgctaaagcg cgtgcgtgat
     1501 ttcgcgcagg tctgcggcga cggtatcgtg acgcggcagg tcgcaaaaca ggcgctcgac
     1561 aggctggaaa ttgatccgct gggccttgat aatatcgacc gcaggatact caccacaatg
     1621 ataaggaact ataacggagg ccctgtcggg cttgaaacga tagcggcgtc aattggcgag
     1681 gagtccgtaa caattgaaga cgtttatgaa ccgtatctga tgcagattgg ttttctgagc
     1741 agaactccgc gcgggcgctg tgtaactccg gccgcttaca aacaccttgg gctggatgcg
     1801 ccggataacg ggcagcagag cttactttaa aaacctacta aaaatgacca tataataatt
     1861 ctgacatata attaattatc agtaataatc gaaaggcggc atctttatgg gaagactttt
     1921 cgggacggac ggcgtcagag ggattgcaaa tactgagctt tcatgcgagc ttgcaatgca
     1981 gcttggaaga gcggccgcga tggtgctgac ggaagaaaca catcaccgtc cgcagataat
     2041 aatcggaaaa gatacgcgaa tttcatcaga tatgcttgaa tcggcgatgg cggcaggaat
     2101 ctgcagcgtt ggcgcgaacg ctcttgtttt aggcgtagtg ccgaccccgg cggtagcgtt
     2161 ccttgttaaa aagctcgggg cggacgcagg cgtcatgata tcggcgtcac ataacccggc
     2221 acagtacaac ggtattaaaa tattcagcgg cagcggttat aaactttcgg acagccttga
     2281 ggaaaaaatc gaggctatcg tactcgatgg agcggaaaag cctccaatca aagcggggga
     2341 cggcgtcggc cgcgttttaa aaccgaaacg ggcggtcgat gaatatatca aatacttaaa
     2401 atccacaaca gaaacaaagt tcaccgggct tcggattgct gtcgactgtg caaacggttc
     2461 ggcttctgtc accgcaccgc tgctttttga ggaaatgggc ggggaatatg aaatactcag
     2521 cgcgaaaccg gacggactta acataaacaa cgcctgcggt tcaacacata tcgagatgct
     2581 gagccggttc gtaaaggaca acgggtgcga catcggcgtt gcgttcgacg gagacgctga
     2641 caggtgtctt gcggttgatg aaaaaggaga actcattgac ggcgacaagt tgatagccat
     2701 tttttcggct tctctcaaaa aacgcggcaa actcaaaagc aatacggcgg ttgtcaccgt
     2761 tatgtccaac ctcggatttt tcaagttcgc ggaaaaacac ggaatcgacg cggcgagaac
     2821 aaaagtcggg gac
//
LOCUS       DATALY010000099         5947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_196456,
            whole genome shotgun sequence.
ACCESSION   DATALY010000099 DATALY010000000
VERSION     DATALY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196456"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VHR42_03000"
     CDS             complement(<1..481)
                     /locus_tag="VHR42_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX3026181.1"
                     /translation="MPRLLFISFIDFGEMKSGSSVRPQRMYDAFLKLGFSVSLLSGLQ
                     NRRRERWRSVFQKFREIRKDPPDLCYVEPPSGPFFNLCDHLLLLYLKHKKIPTGLFYR
                     DAYWLVPKRWQVGGLKKFVLNQCEKFDFLIIKDSCSVVFFPTSSMAEKVSLPCKSLLP
                     "
     gene            complement(496..1815)
                     /locus_tag="VHR42_03005"
     CDS             complement(496..1815)
                     /locus_tag="VHR42_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3026182.1"
                     /translation="MLNALKKTVKYMVYLLPPVVLFGSFIEFGRIPGAGAVTFSRVYW
                     LVLVVLALMLAVKTGFSSRQLGAAKKYCLFFILMIGYAALSLIWVRNRGSSFGNEFIF
                     QFVGILTVLILTFMLTSLFDLRIFLRVVAVCYAITVLLGIYEIFTGNFGFTPSYKAYI
                     LRNDYGLFFPYAEFYNTNDYASFVSMFLPFAGYSAVNSWKGPGGKILACVGAAAGIFT
                     VFCANARICYFAIVLFVVTLLLALMFKESLKKFRRRILLTFASGFGIIVLLAATSLVH
                     IKVLLNELRSINFNDHSIKIRFDLIRGAFRMLWHSRLMGVGVGNSVALIPQYSTLKPI
                     NLHNLPLQILTEYGIIIFALYVLMTVGAAKRYFKYQGSVRTDVFASICFASVLELQLV
                     GMASSDMMRVTGMWMIFGIIIVGIKVLYPVTDSVASNEDSEAENKEK"
     gene            complement(1838..2968)
                     /locus_tag="VHR42_03010"
     CDS             complement(1838..2968)
                     /locus_tag="VHR42_03010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026183.1"
                     /translation="MKVLIVPSWYVTEDSPNNGIFFQEQAQALAQSGLEVSVIYPDLR
                     FRLGELRKGLFRVEDAPVPTWIYRRRTLTPFWERGRWPQRALMLERLYFNNCREYGRP
                     DVVHLHSCRMGVEVLGLCRRHNLPLVYTEHYSGVTGNMNAALRYAFHKTLSGCDAALA
                     VSRNLRTIMTGIRSDVRYLPNMVDTDRFTIQKPAGSGGFRFGAVGNLIPVKGFDLLLR
                     AFAEALPRMDGATLVIAGAGRLRGNLERMARELNLAGRVEFSGFVKRGELPGFYHRLG
                     CLICSSRRETFGVTLIEALACGKPLIATRCGGPESIVTPRNGLLVKNGDAGDLARAME
                     EMYQHHKSYDPASLRLECEEEFGSRRVCDKITEVYRRVLRNG"
     gene            complement(2976..4307)
                     /locus_tag="VHR42_03015"
     CDS             complement(2976..4307)
                     /locus_tag="VHR42_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3026184.1"
                     /translation="MFSKQKLSGLLRYAGQGIKNGFLHILVGNTLVKFISMFSSILIV
                     RLLTKDQYGSLSVVQNILNYALLFNGLGMANAVLRYCSVYDEPGMKKGYFSFAVRFGL
                     WADAGLVAVLLSGLYLLNRFRFYQISADTRRILFALALTPLLQFLFDAIQGFFRANQE
                     NKNYSKTSVIFTACFGIFQVIFAIFFKLDGIVAGRYAAFLVTVWIGFRLLRRMGAFQV
                     KGVRISRPDRSEAVKYAVNSMFCNIFSLILPLNETLIVYHFVSNDSARADFNAATLAP
                     ASIQFITMSVIVFVFPYFAKNYKDGKWVMNNAKKLYLGLTAAMVVICGACIILTPQII
                     HLFFSKKYLTPSAVGLMRVFWVTFGINSALRMPTGNILASIGEIKFNLYNAVLAFTVH
                     LAVCWFLVKHYGINGGAYGLLTVYALSSVLGIFYLRRYCKKLEKAKLEEAS"
     gene            complement(4312..5422)
                     /locus_tag="VHR42_03020"
                     /pseudo
     CDS             complement(4312..5422)
                     /locus_tag="VHR42_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(5465..>5947)
                     /locus_tag="VHR42_03025"
     CDS             complement(5465..>5947)
                     /locus_tag="VHR42_03025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3026185.1"
                     /translation="GTGAVLYAGSVIADRVLIADLATVREKVIIGKKTIVGRGVCIEN
                     CCNIGCECKLETNSYLCAYSSVEDGCFIAPGVVTSNDNYAGRSRKRFSCFKGLTLKKG
                     ARIGAGAVVLPGREIGPDGFAAAGSVVTRDIEPGTIAAGCPARELRKVPEDQLLENQK
                     "
BASE COUNT         1598 a         1655 c         1397 g         1297 t
ORIGIN      
        1 ggggcagcag gcttttgcag ggaagactga ccttttccgc catactggat gtgggaaaaa
       61 acaccacgct gcagctatct ttgataatca gaaaatcaaa tttttcacat tggttgagaa
      121 caaacttttt aagccccccg acctgccacc tcttcggaac cagccagtag gcgtcccggt
      181 aaaacagccc ggtcggaatt tttttgtgtt taagatacag cagcagcaaa tgatcacaaa
      241 gattaaaaaa agggccgctt ggcggctcga cataacacaa atcgggagga tcttttcgaa
      301 tctcccggaa tttttgaaaa acactgcgcc accgctcgcg cctccggttt tgtagtccgg
      361 acagcaggga aacagaaaag ccgagcttta aaaaagcatc atacattctc tggggcctga
      421 cgcttgagcc tgatttcatt tcgccgaaat cgataaaact gataaacagt agccggggca
      481 tcgaagcacc cccttttatt tctctttatt ttccgcttcc gagtcttcgt ttgaagcgac
      541 gctgtcggtc accggataaa gcaccttgat tcccacaatt ataatgccga aaatcatcca
      601 cattccagta acccgcatca tgtcggaaga cgccattccc accagctgta attccagcac
      661 agaagcaaag cagatgcttg caaacacatc cgtcctgaca gagccctgat atttaaaata
      721 gcgtttggcc gcacctaccg tcatcagcac ataaagagca aatattatga taccatactc
      781 ggttagaatc tgcaacggca ggttatgcag attaatgggc ttaagcgtgc tgtactgcgg
      841 gataagcgcc acgctgtttc cgacgcccac ccccatcagg cgggaatgcc aaagcatgcg
      901 gaaagcgccc cggatcagat caaaacggat tttaatggaa tgatcgttga aattaatcga
      961 cctgagttcg ttgagaagca cctttatatg aaccagggat gtcgccgcca gcaggacgat
     1021 aatcccaaag ccggaagcaa aagtcagcag aatcctccgg cggaattttt tcaggctttc
     1081 tttaaacatc agcgcaagca gcagcgtgac aacaaaaaga acaattgcaa aatagcaaat
     1141 ccttgcgttg gcgcagaaaa cagtgaaaat acccgctgct gcgcccacgc aggccaaaat
     1201 ctttccgccg ggccctttcc aggaattcac cgccgaataa cctgcgaacg gcaaaaacat
     1261 agaaacaaaa gaagcatagt cgttggtatt ataaaattcg gcatagggaa aaaacaggcc
     1321 gtaatcgttt cttaaaatgt atgccttgta actcggcgtg aacccgaaat taccggtaaa
     1381 tatctcataa atccccaaaa gcaccgtaat ggcgtagcaa acagccacca cgcgcaaaaa
     1441 tatcctcaaa tcgaaaagac tcgttaacat gaacgtcagg atcagcacag ttagaatacc
     1501 gacaaactga aagataaatt catttccaaa tgacgatccc ctgtttcgaa cccagatcag
     1561 cgacagcgcc gcgtagccga tcatcaagat aaaaaacaga cagtattttt tagcggcgcc
     1621 cagttgccgc gagctgaagc cggttttaac ggcaagcatc agggccaaga cgacaagaac
     1681 cagccagtaa acacgggaaa acgtgactgc accggcgccg ggaattctgc cgaactcaat
     1741 aaaagagccg aacagaacta ccggcggcaa gagataaacc atatatttaa ctgtcttttt
     1801 taaagcattc agcataaaac gctcctgtca gtgaatttca gccgttgcgc agcacccggc
     1861 ggtagacttc cgtaatttta tcacaaactc tccggctgcc gaattcctcc tcgcattcca
     1921 gacgcaggga tgcggggtca tagcttttat gatgttgata catttcttcc atcgcacggg
     1981 ccaaatcgcc tgcatccccg tttttcacca atagaccgtt gcggggtgtg acgatgctct
     2041 ccgggccacc gcagcgtgtg gcaatcagcg gttttccgca ggccagcgcc tcaatcaggg
     2101 ttaccccgaa ggtctctctg cggctggagc atatcaggca gccgagcctg tgataaaaac
     2161 cgggcagctc tccgcgcttt acaaaaccgg aaaattcaac cctgcccgca agattcagtt
     2221 cccgggccat ccgctccaga ttgcctctga gccgcccggc ccccgcgatc acaagggtag
     2281 caccatccat ccggggaagc gcctcggcaa aagcgcgaag caataaatca aagcctttga
     2341 ccggaatcaa atttccaacc gcgccaaacc gaaatccgcc ggaaccggcc ggtttttgaa
     2401 tggtaaagcg gtcggtatcc accatgttgg gcaggtagcg cacatcgctt cgaattccgg
     2461 tcatgatcgt tcttaaattc cggctgacgg caagcgcggc gtcacagccg ctcagtgttt
     2521 tatggaacgc gtagcgcagc gccgcattca tattcccggt cacacccgaa taatgctcgg
     2581 tataaaccag cggcagattg tgccgtctgc ataaacccag cacttcaacg cccatccggc
     2641 aggaatgaag gtgaaccaca tccggtcttc cgtattctct gcaattattg aaatacagcc
     2701 gttcgagcat cagcgccctc tgcggccatc tgccgcgttc ccagaaagga gtgagcgtac
     2761 gcctgcggta aatccatgtg ggaacagggg cgtcctccac ccggaaaagg cccttcctca
     2821 gttcgcccag ccggaaccgc aggtcgggat aaatcaccga aacctccagc ccgctctgcg
     2881 cgagggcctg cgcctgctct tgaaaaaaga ttccattgtt ggggctgtct tccgtcacat
     2941 accaggacgg cacaatcaaa actttcataa atcccctatg acgcctcttc caattttgcc
     3001 ttctccagtt ttttgcagta tcgtctgaga taaaagatgc caagaaccga agacagggcg
     3061 taaaccgtca acagcccgta ggcgccgccg ttgattccgt aatgtttgac caaaaaccag
     3121 caaaccgcga gatgaacggt aaaagcaagc acagcgttat aaagattgaa cttaatctcg
     3181 ccgatcgacg ccagaatatt tcccgtcggc atgcgcagcg cgctgttgat gccgaacgtg
     3241 acccaaaaca cccgcatcag gccgactgcg gagggcgtta gatatttttt actgaaaaac
     3301 aggtgaatga tctggggcgt caatataata caggccccgc agatgacaac catcgcggcg
     3361 gtcagcccca aatagagctt tttggcgtta ttcatcaccc atttgccgtc tttataattt
     3421 ttggcgaagt aagggaaaac aaacacgatt accgacatcg taataaattg gatactggcc
     3481 ggagcaagcg tagcggcgtt aaaatccgct cttgccgagt cattggatac aaaatgataa
     3541 acaatcagtg tttcgtttaa cggcagaatc agtgaaaata tgttgcaaaa catcgagttc
     3601 accgcgtatt tcacggcttc cgaccggtca ggccttgaaa ttcgcacccc ttttacctga
     3661 aaggctccca tacggcgaag taaacgaaag ccaatccaga cggtcacaag gaacgccgcg
     3721 taacggcctg ccacgatccc gtccagtttg aagaatatcg cgaagatcac ctgaaagatc
     3781 ccgaaacatg cggtaaaaat caccgacgtt ttggaatagt tcttattctc ctggttcgct
     3841 ctgaaaaaac cctgaatcgc gtcaaacaag aactggagca acggggtcag cgcaagggcg
     3901 aacaggattc tccgggtatc ggcgctgatc tggtaaaacc gaaagcggtt gagcaaatac
     3961 aaccccgaga gcagaaccgc caccagcccc gcgtcggccc acaggccaaa ccggacggca
     4021 aaactgaaat accccttttt catgccgggt tcatcataaa ccgagcaata ccgaagcacc
     4081 gcgtttgcca tgccgagccc attaaaaagg agcgcatagt ttaaaatatt ctgaaccacg
     4141 cttagagacc cgtattgatc ctttgtcagc aggcgcacga taagaatgga ggaaaacatc
     4201 gagataaatt ttacaagcgt attgcctaca aggatatgca gaaaaccgtt tttgattccc
     4261 tgacccgcat atcgcagcaa accgctcagt ttttgttttg aaaacatgtt ctcacccgtt
     4321 aactttaggg gaagagaatc ttccgctgaa atcggaggac gaacaatgga caagcggaag
     4381 cgaaaccggc cggccttccg ccgcgctttg atagatggcg agcacaagct ccagcgcacg
     4441 ccgccccgca tgaccgtcga cataaggttc cctaccgtca cggatggcgg ccgcgacgtc
     4501 ggcgtaaagc ggcgtatggc cgaaaccata gacgttcggc ggattctctc caaattcggc
     4561 catgatccgg gctgagtcgt caaggccgtc ggcaaagcgc cattcttcaa tccggttgac
     4621 cgatttgccg ccagctttca cagttccctt ttctccaaaa atataaaggg tttcctccaa
     4681 atttttaggg tagatattgg tagtgccttc aatcagcccg taggatgaat tgcggaattt
     4741 gaccagcgcg aggccaagat cctcggcctc gatgtaaggg tggctgagcc ggtcggtata
     4801 agcggtgact tccgccacct catcccccat catccagcgc agaaggtcaa tgttgtggat
     4861 acattgattc atcagcgcgc cgccgtcgct tacccatttt cccctccacg gtgcctgaac
     4921 ataataatcc tcgccccggt tccaacgaat atgagcggcg gcgtgataaa gccgcccaaa
     4981 ccggccggat tcgagcgcag cgcggatttt cagaattgat ttgttaaacc ggttctggtg
     5041 gcaggcgcag agtttcactc ccttttgagc cgcggcatca atcagccggt cagcgtcatc
     5101 aagggaaagc gccagcggtt ttttccacga ttacattgca gcccgcattg atacaatcga
     5161 gcgcaacggc ggcgtgagag ccgctctcca ccgcgacggc ggtaaggccc ggcttttcgt
     5221 tttgcagcat cagccggtaa tccgtataaa ccgggatttt tctgtcgcca agattaaaat
     5281 cccggatcaa tttttccgct ttttctttct ctgtatcaca gagagcaatg atttcaaagt
     5341 cggcgctgtt gttcagcgcg gcggcaatgt ggttgggtga tattctgccg cagccgatca
     5401 gcgcatatct cagtttaccc atgaaattct ttcctttccc gcgggacaat cctttaattt
     5461 cgatttattt ctgattctcc agaagctgat cttccggaac tttccggagc tcccgtgccg
     5521 ggcagcccgc cgcaatcgta ccgggctcaa tatcccgggt aacgacgctg ccggcggccg
     5581 caaaaccatc cgggccaatc tctcttcccg gaagaaccac cgctcccgcc ccgatccggg
     5641 ctcccttttt cagtgtcaat cctttaaaac acgaaaatct cttccgggag cggccggcat
     5701 aattatcgtt ggaggtaaca acgcccggag cgataaagca cccgtcttca accgaggaat
     5761 aggcacaaag gtaggagttt gtttccagct tgcactcaca gccgatatta cagcagtttt
     5821 caatacatac accccggccg acaatggttt ttttgccgat gatcaccttt tcccgaacgg
     5881 tcgccagatc agcgatcaga acacggtcgg caatcacgct tccggcgtaa agcacggccc
     5941 ccgtacc
//
LOCUS       DATALY010000100         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_628119,
            whole genome shotgun sequence.
ACCESSION   DATALY010000100 DATALY010000000
VERSION     DATALY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628119"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..553
                     /locus_tag="VHR42_03030"
     CDS             161..553
                     /locus_tag="VHR42_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3026186.1"
                     /translation="MFQEVFLRLMKEEKPFFGEEHLKAWLIRVTINCSRKIFCSSWFR
                     KTVPLDEKIPVEWSEQSETLDAVLSLPKTDRTIIYLYYKEDYNLKEIAAILHMNESTV
                     RSRLSRARTKLREKLGGVPDEGQRQGSI"
     gene            525..1343
                     /locus_tag="VHR42_03035"
     CDS             525..1343
                     /locus_tag="VHR42_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026187.1"
                     /translation="MKGSVREAFEMVHVPEQLRQSTSEKMLTAAKKKRTVWKAAAAFC
                     MILLILAGTMFFPGNNGKPDGLAFSVYAADGKDHKEIPLSESAVQITLDNDKKYRSGG
                     VGGVGNSETMTWEELIDFNLHCTGRGDIKHVTYTAHNCEFLKKSFFTQEQKKDGEASD
                     YADGSLWSETDQAGNETTWGFSPVGYVYSADYMEQVNLKNFGLKITYEESKPKGYRPF
                     TVEESAKFEKLREQAYENAYISAEIELENGRVISKTIKIGFLPGYGKVTAWIVG"
     gene            complement(1426..>1680)
                     /locus_tag="VHR42_03040"
     CDS             complement(1426..>1680)
                     /locus_tag="VHR42_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX3026188.1"
                     /translation="RINHPSFPNTLAGVIYQPGAFSCLDDGGINAQVSDSAYKAARDA
                     MNGWDPCGGAIYYYNPAKTTNKWIWARPVIVVIGKHRFCS"
BASE COUNT          485 a          338 c          457 g          400 t
ORIGIN      
        1 taagcttgat gaaaggtatt tctgtaagag gggggcgatg cgatggcaat tcagccggag
       61 cgaacccggg attttgtgaa ttcggtactg gaacggtatt cggatatggt gtaccgcctt
      121 gctttggcgc gtacaaaaaa agaaagaaga cgccgaagat gtgtttcagg aggtttttct
      181 ccggttgatg aaagaggaaa agcccttttt tggcgaggaa cacctcaagg cttggctgat
      241 tcgtgttacc attaactgct cccgcaaaat tttttgttcc tcatggttcc gtaaaaccgt
      301 gccgctagat gagaaaatac cggtggaatg gtcggaacag agcgaaacgc tggatgctgt
      361 tctctctttg ccgaaaacag ataggactat tatctatcta tactacaaag aggactacaa
      421 tttaaaggaa attgcagcta tcctgcatat gaacgagtcg accgtccgat cacgtttgag
      481 tcgtgcaagg actaaacttc gtgagaagct gggaggggtg ccggatgaag ggcagcgtca
      541 gggaagcatt tgaaatggtt cacgtgccgg agcagctgcg ccagagtaca tcggaaaaaa
      601 tgcttactgc tgcaaaaaag aaacgaacgg tatggaaggc tgccgcggct ttttgcatga
      661 ttcttctaat attggcagga accatgtttt tcccgggaaa taacgggaaa cctgacggac
      721 tggcttttag tgtttacgca gcagacggaa aggatcacaa agaaattcca ctctccgaaa
      781 gcgcagtgca gattacatta gataatgata aaaaataccg ctcgggcggc gtgggcggcg
      841 tcgggaacag cgaaacgatg acgtgggaag aactgattga ttttaatctc cactgtactg
      901 gccgtggaga tattaagcat gtaacttata ccgcccataa ctgtgaattc ctaaaaaagt
      961 ctttttttac acaggaacag aaaaaagacg gcgaggcgtc cgattacgca gatgggtctt
     1021 tatggagcga aacggatcag gccgggaatg aaaccacatg ggggttttca ccggtgggtt
     1081 atgtatattc tgccgattat atggagcagg taaatttgaa gaattttggt ttgaagatca
     1141 cttatgaaga aagcaaaccc aaaggataca gaccgttcac tgtagaagag agtgcaaaat
     1201 tcgaaaaact ccgggaacag gcatacgaga atgcgtatat ctctgcggaa attgaacttg
     1261 aaaacggaag agtcatcagc aaaacaataa agatcggttt cctgccggga tatggaaaag
     1321 taacggcatg gattgtaggc tgattgtcaa ctttatctgc agcatatcac aaaaaggagc
     1381 aggaattctt tcctgctcct cggtatttac aaagatggga atgactcagc tgcagaatct
     1441 gtgtttgccg atcacgacaa taaccggccg cgcccagatc catttgtttg tcgttttggc
     1501 cggattataa taatagatag cgccgccgca tgggtcccag ccgttcatag catcgcgggc
     1561 ggccttgtag gcggaatccg atacctgagc gttgatgccg ccgtcgtcga ggcacgagaa
     1621 agcaccgggc tgataaatca cgcctgcaag cgtattcggg aacgatgggt ggttgatgcg
//
LOCUS       DATALY010000101         4277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_196825,
            whole genome shotgun sequence.
ACCESSION   DATALY010000101 DATALY010000000
VERSION     DATALY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196825"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /gene="ppdK"
                     /locus_tag="VHR42_03045"
     CDS             complement(<1..1769)
                     /gene="ppdK"
                     /locus_tag="VHR42_03045"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX3026189.1"
                     /translation="MRELLGGKGANLAEMTNLGMPVPQGFTISTEACTKYYDDGQTIS
                     AEIQNQIYEDLGKMEKICGKKFGDINNPLLVSVRSGARASMPGMMDTILNLGLNDTVV
                     EGVKKLTNNERFAYDSYRRFIQMFSDVVMELPKSNFEKIIDKMKADRGVKLDTELNAA
                     DLKELVKLFKEYYKSQKGVDFPTDPKEQLMESVKAVFRSWDNPRANVYRRMNDIPYSW
                     GTAVNVQMMVFGNMGDDSGTGVAFTRNPATGEKKLYGEFLINAQGEDVVAGIRTPQTI
                     DQLANVMPEVYNEFAGFANKLEQHYHDMQDMEFTIEKGKLYMLQTRNGKRTAAAALKI
                     AVDLVAEGMLTKEQALLKVEPKQLDSVLHPQFDPKAIKAAKPIARGLAASPGAACGKV
                     VFTAEDAADWAQNKKEKVILVRLETSPEDIVGMAVSEGILTVRGGMTSHAAVVARGMG
                     TCCVSGCGEINMHEEEKYFEIGGKTIKEGDYISIDGSTGNIYAEAIPTAPATITGDFD
                     KFMTWADEIRTLMVRTNADTPKDAEQAFKFGAQGIGLCRTEHMFFDPDRIPAMREMIV
                     AKTEQQRRKALDKLLPMQRSDFEG"
     gene            2232..3611
                     /gene="glmU"
                     /locus_tag="VHR42_03050"
     CDS             2232..3611
                     /gene="glmU"
                     /locus_tag="VHR42_03050"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX3026190.1"
                     /translation="MEKTAALILAAGEGKRMKSAKAKVLHEVLFKPMLDWVSDSVLES
                     GIEEICIVTGHLSEQVEEHIGGRFSTVRQTERLGTAHAVLQAKNFLLANGIRDVVILC
                     ADAPFVGSDVLKESYALHKSSQNAVTVITAETEDPYGYGRILRDGEGNVCRIAEEKDA
                     TSEEKSIREINSGAYWFEVSALLDAIARIKNKNAKGEYYLTDAVALLAEAGRKCGAVR
                     SDMSVTAAANDRRQLMELNAKARRAALDRLYEEGVSIVDESGIIVGPDVKIGRDTTLL
                     PGTILRGKCVIGQGCVIGPNSLIDSCEFGDEVVFNASQAYRSKIGSRATIGPFVHIRP
                     NSVLHEKVHVGDFVEIKNSEIGEGTKVPHLTYVGDSDVGGGVNFGCGCVTVNYDGVSK
                     SRTKVGDGAFIGCNTNLVAPVEVGKNAYTAAGSTITRDVPENALAVARSRQQNLEGWV
                     TKKRPAKKN"
     gene            3753..>4277
                     /gene="pth"
                     /locus_tag="VHR42_03055"
     CDS             3753..>4277
                     /gene="pth"
                     /locus_tag="VHR42_03055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX3026191.1"
                     /translation="MVAGLGNPGKEYEGTRHNAGFIALDSISQSLNVRLDRARFKSCC
                     GIASVGGKKALLMKPQTFMNLSGEAVAEASSFYKIPMEKVIVIYDDISLPVGKIRIRP
                     KGSAGGHNGIKNIISLTGTDVFPRIKIGVGQKPRPDYDLVDWVLGRIPQTEKEQFAQA
                     VESTARAAEMIIQEN"
BASE COUNT         1028 a         1013 c         1191 g         1045 t
ORIGIN      
        1 ccttcgaagt cgctcctctg catcggaagc agcttgtcaa gagccttcct gcgctgctgt
       61 tcggtttttg cgactatcat ttcgcgcatt gccgggatcc tgtcaggatc gaagaacata
      121 tgctcggtgc ggcaaaggcc gatgccctgt gcgccgaatt taaacgcctg ctcggcgtcc
      181 ttgggggtgt cggcgttggt gcggaccatc agggtgcgga tctcgtcggc ccaggtcatg
      241 aacttgtcaa aatcgcccgt aatggtggcg ggagcggtcg ggatagcttc tgcataaata
      301 ttgccggtcg agccgtcaat agaaatgtag tcgccttcct tgattgtttt gccgccgatt
      361 tcaaaatact tttcttcttc gtgcatgttg atctcgccgc agccggaaac acagcaggtt
      421 cccataccgc gcgccacaac agccgcgtgg gaggtcatgc cgccgcgaac ggtcaggatg
      481 ccctcggaga cagccattcc gacgatatct tccggagaag tctcaagacg aacaagaatg
      541 actttctctt ttttgttctg tgcccaatcg gcagcgtctt cagcagtgaa gacaactttg
      601 ccgcaggcgg ctccggggga tgctgcaagg cctctcgcaa taggtttcgc ggcctttatg
      661 gctttggggt caaactgggg atgaagcacg ctgtcaagct gcttgggttc aaccttgaga
      721 agagcctgct cttttgtgag cattccttcg gcaaccagat caacggcaat tttcagagcg
      781 gcagcggcgg tacgtttgcc gttacgggtc tgaagcatat agagtttgcc tttttcaatg
      841 gtgaactcca tatcctgcat gtcgtgatag tgctgttcga gtttgttggc gaaaccggca
      901 aattcgttgt aaacttccgg cataacattc gccagctgat cgatggtctg cggcgtacgg
      961 atgcctgcaa cgacatcttc gccctgagca ttgatcagga attctccgta gagtttcttt
     1021 tcgccggtgg cggggttgcg ggtaaaggca acgcctgttc cggaatcatc gcccatgttt
     1081 ccgaatacca tcatctggac gttgaccgcg gtgccccagc tgtaaggaat atcgttcatg
     1141 cggcggtaaa cgttagcgcg ggggttgtcc cacgaacgga aaacagcctt gacggattcc
     1201 atgagctgct ctttcgggtc ggtcgggaaa tcgacgccct tctgggattt gtaatattct
     1261 ttgaaaagct ttacaagctc tttcaaatcg gctgcgttaa gctcggtgtc gagttttacg
     1321 cccctgtcgg ctttcatttt atcaatgatt ttctcgaagt ttgatttggg gagttccatg
     1381 acaacatccg agaacatctg aataaaacgg cgataggaat cgtaagcgaa tctctcgttg
     1441 ttggtcagct ttttgacgcc ttccacaacg gtgtcgttca ggccgaggtt cagaatggtg
     1501 tccatcatgc cgggcatgga tgcgcgtgcg ccggaacgta cggaaaccag gagaggattg
     1561 ttaatgtcac cgaatttttt gccgcagatc ttttccattt tgccaagatc ttcgtaaatc
     1621 tggttctgga tttcagccga aatggtttga ccgtcgtcat agtatttggt gcaggcttcc
     1681 gtagaaattg taaagccctg aggaaccggc attccgagat tggtcatttc cgccaggttg
     1741 gcgcctttgc ctccgagcaa ttcgcgcata tgctcgttgc cttcagagaa caagtagaca
     1801 tatttgtgac tcaatgggat caacctcctg taatttagaa taaatataaa aatgatagat
     1861 atagattatg aagtagtcac ataatcaaat ggaattatac cacgattggc agaatctttc
     1921 tataatttca tcgaaaaatt ttacaaaatt ccgaagaata tcagaaaaaa atgatcataa
     1981 tttacatttt gccaacaatt ttaatataat agtgatagaa agcgcctgcc gcgcggcttt
     2041 tttccttgat catcggagac ggcgggccgg atttgacggc gaatacccga tcgggcttga
     2101 aatcgacggt taaaactgcg ataataattc agggttgttt tataatggcc gcaaattttc
     2161 ggattttccc cggagtggga aaattgtttc aaccgataaa taatcaataa agaaattggg
     2221 aggcattctt cttggaaaag actgctgcgt taatactggc cgccggagaa ggaaaacgga
     2281 tgaaatcggc aaaggccaag gtgcttcatg aggtattgtt taaaccgatg ctggactggg
     2341 tgtcggactc cgtcctcgaa tccgggatcg aagaaatctg cattgttacc ggtcacctga
     2401 gcgagcaggt ggaggagcat atcggcggac gcttttcaac tgtgcgtcag actgaaaggc
     2461 ttggaacggc ccatgctgtt ctgcaggcga agaactttct gcttgcgaat ggaatcaggg
     2521 atgtcgtcat cctctgcgca gacgcgcctt ttgtcggctc cgatgttttg aaggagtctt
     2581 acgcgctaca taaaagttcg cagaatgcgg tgacggttat tacggcggag acggaagatc
     2641 cttatggtta cggaagaatt ctccgggacg gggaaggcaa tgtctgccgg atcgcggagg
     2701 aaaaagacgc gacgagtgaa gaaaaatcca tccgtgagat caactcgggt gcttactggt
     2761 ttgaagtgag cgcgctgctt gacgccattg cccggattaa aaataaaaac gccaaaggcg
     2821 agtactatct gaccgacgcg gttgcgcttc ttgcggaggc gggccggaaa tgcggagcgg
     2881 tcaggtcgga tatgtcggtt acagcggcgg caaacgaccg ccggcagctc atggagctta
     2941 acgccaaggc ccgcagggcg gcgcttgacc gcctttatga agagggcgtg tccatcgtgg
     3001 atgagtcggg tattatcgtc gggccggatg taaaaatcgg ccgtgacacg acccttcttc
     3061 ccggaacgat tctaaggggc aaatgcgtga tcggtcaggg ctgcgtgatc ggcccgaatt
     3121 ccctgattga ttcctgtgaa tttggcgacg aggtggtttt caacgcatcc caggcctatc
     3181 ggtcgaagat cgggagcaga gcgaccattg gtccgttcgt ccatatccgg ccaaattcgg
     3241 ttttgcatga aaaagtgcat gtcggcgatt ttgttgagat caagaattcc gaaatcggcg
     3301 aaggcaccaa ggttccccat ctgacctatg tcggggactc cgacgtagga ggaggggtca
     3361 attttggctg cggctgcgtt acggtcaatt acgacggggt ttccaaaagc cgcaccaaag
     3421 tcggagacgg cgcattcatc ggctgcaata ccaatctggt tgctccggtt gaagtgggga
     3481 aaaacgccta taccgccgca ggctcgacca ttacccggga tgttccggag aacgcactgg
     3541 cagtggcccg ttccagacag cagaatcttg agggctgggt tacaaaaaaa cgcccggcga
     3601 aaaagaactg acgactgacc tgcataacga tataacaacc cgcgttccac agggaagcgg
     3661 gcggatggtg gaaagatttt ggaaagactt aaattattgt ttgaccgtat tcagcaagag
     3721 aaatcgctgc ccgggggggg gctcccgagt atatcgttgc gggcctcggc aatcccggaa
     3781 aggaatatga aggcacccgt cataacgccg gttttatagc gctggattct atttcgcagt
     3841 cgctgaatgt ccggcttgac agggcccggt ttaaatcctg ctgcggcatt gcgtcggtcg
     3901 gcggaaagaa agctctcctc atgaagcccc agaccttcat gaatctgagc ggcgaagcgg
     3961 tggccgaggc ctcctctttt tataagattc cgatggaaaa ggtgattgtc atttacgacg
     4021 acatctcgct cccggtagga aaaataagaa tccgtcccaa aggcagcgcc ggcggccaca
     4081 atggaatcaa aaatattatc agcctgacgg ggaccgacgt ttttccaaga atcaaaatcg
     4141 gggtcggtca gaaaccgaga cctgattatg atcttgtcga ctgggttctg ggcagaatcc
     4201 cgcagacgga aaaagaacaa ttcgctcagg cggtcgagag taccgcccgg gctgccgaaa
     4261 tgataatcca ggagaat
//
LOCUS       DATALY010000102         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_456043,
            whole genome shotgun sequence.
ACCESSION   DATALY010000102 DATALY010000000
VERSION     DATALY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456043"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(692..1756)
                     /gene="mreB"
                     /locus_tag="VHR42_03060"
     CDS             complement(692..1756)
                     /gene="mreB"
                     /locus_tag="VHR42_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEX3026192.1"
                     /translation="MPGSDIGIDLGTATILVYIKGKGIVLKEPSVVAVDKDTDEIVAV
                     GLEAQKMLGRTPGNIIAVRPLREGVISNFNLTEQMLKYTIKKVGSHLLFKPRMMICVP
                     SIITEVEERAVVDAALQAGARKAYLIEEPVAAAIGAGIDISKPCGSMIVDIGGGTTDI
                     AVLSLKDVAISTSIKVAGDKFDEAVVRYIRRRYNVLIGERTAEEVKIKIGCVYPQEER
                     RIMAVKGRSLVTGLPKSLQVTSDEIMEAFEDPANAIYEAIHSVLESTPPELVGDISTN
                     GILLTGGGSLIRGLDQLIMKRVGIPAHIAEDPVSCVAIGTGKALDKLGDLKDGLLYIS
                     KNKTLTKEKKESRDREKRGS"
BASE COUNT          421 a          476 c          404 g          481 t
ORIGIN      
        1 caggattata gctattgtcc aaactatggg tataaggatt aaatattagt taagcaaatg
       61 caaagatctg cgccgtattt tatacaaaag ataagccgtg aaatggatac gggcagtaca
      121 aacccccacg gggaaatcca atctccgtgg ggttcttcta tataatttgc cctgcaatga
      181 aaaactttcc gaataccctt gcataattcg cccaaaaata ctataataag cattaggaaa
      241 ctctgaaaat taaaaatcgc ggcaggcgca tgcggaagca acgcatgtac cctgcattca
      301 gatgtagaga ccacctgacg caaccggatt cccggcacca cgactttctt attataaact
      361 gagtttcctt ttttcaagtg ggattattag ggagaacgca attttcctct gcgctctccc
      421 cataaactga atgaccctcc gcaaaatcgt aagcaagtat caggggccgt gccttgtcgg
      481 tgtttggttc gcaagctttg aaataatgaa gcacgtgcgg ggttaaagcc tgttcatgct
      541 ttttggtttt tcagccgccc ttttatcgcg gcagaattcc cattattctg caggaagcct
      601 gttcaggccg gtatatccgg gttggatagg ctttcttgct gaatattgaa tacagcgcag
      661 gagactgtca aaaaggggcg aaaccgtgac gttagcttcc gcgtttttcg cggtcgcggg
      721 attctttttt ttcttttgtc agggttttgt tttttgaaat atacaacagc ccgtctttta
      781 aatcgcccag cttatcaaga gccttgccgg ttccgattgc gacgcagctc accgggtctt
      841 ccgcaatatg ggccgggatg ccgacccgtt tcataatcaa ctggtcaagc ccgcgaatca
      901 ggcttcctcc gcccgtaagt aaaatgccgt ttgtggaaat atccccgacc aactcaggcg
      961 gtgtgctctc caaaacggaa tggatggctt cgtaaatggc gttcgccgga tcctcaaacg
     1021 cttccatgat ttcgtccgaa gtaacctgta acgatttcgg caggccggtc acaaggcttc
     1081 ggccttttac ggccatgatt cttctttctt cctgcggata gacgcatcca atcttgattt
     1141 tcacttcttc cgcagtccgc tcgccgatga ggacattgta tctcctgcgg atgtatcgga
     1201 cgaccgcttc gtcaaattta tctcccgcca ctttgatgga ggtggaaatt gcgacgtctt
     1261 ttagggacag caccgcgata tccgtcgtgc cgcccccgat gtcgacaatc atgcttccgc
     1321 atggcttgga aatgtcgatc cccgcgccga tggccgccgc gaccggctct tcaataagat
     1381 acgcctttcg ggcacccgcc tgcaaggcag cgtcaacaac cgcccgttcc tctacttcgg
     1441 taataatgct tggcacgcaa atcatcatac gcggtttaaa aagcaagtga gagccgactt
     1501 ttttgatcgt gtatttcagc atttgctcgg ttaagttgaa gttggaaatc acgccttccc
     1561 gcaaaggccg gactgcaata atattgccgg gcgtcctccc gagcattttc tgtgcttcca
     1621 gcccgacagc tacgatttca tccgtgtcct tgtctaccgc cacaacggac ggctccttga
     1681 gaacgatgcc ttttcccttg atgtatacca aaatcgtagc agtccccaag tcaatcccga
     1741 tatcgctgcc cggcatttct ccaccccgca ttacttattc cg
//
LOCUS       DATALY010000103         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_541794,
            whole genome shotgun sequence.
ACCESSION   DATALY010000103 DATALY010000000
VERSION     DATALY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541794"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..747)
                     /locus_tag="VHR42_03065"
     CDS             complement(313..747)
                     /locus_tag="VHR42_03065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3026193.1"
                     /translation="MEFKWTKGLEFFQDAHEIREKVFVKEQGFEHEFDQTDHEALHVV
                     LYLDGVPVATARLFSEQGRVFHVGRVAVLKDYRGSHLGAALMKEVVRKAEQLGAEKIV
                     LGAQKQAEPFYRKLGFLSENDCYFDEGCPHVNMFLKLNCQKG"
     gene            complement(819..1067)
                     /locus_tag="VHR42_03070"
     CDS             complement(819..1067)
                     /locus_tag="VHR42_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX3026194.1"
                     /translation="MVNYITSQKVEIDIHNMKRAEAKTFLERFLSNANGNVKEIIVIH
                     GYASGTVLRDMVRRSLKHRRIKQKIISLNPGVTTLLLD"
     gene            complement(1260..3077)
                     /gene="typA"
                     /locus_tag="VHR42_03075"
     CDS             complement(1260..3077)
                     /gene="typA"
                     /locus_tag="VHR42_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX3026195.1"
                     /translation="MVRSDLRNIAIIAHVDHGKTTLVDEMLKQGGVFRENQYVQERVM
                     DSNDLERERGITILAKNTAATYNGIKINIVDTPGHADFGGEVERILKMVNGVLLLVDA
                     AEGPMPQTRFVLQKALALGHKVIVVVNKIDKPDSRIREVENEVLELFLDLNANEEQLD
                     SPFVYCSGRQGTASLKPDEEGTDLKPLFDKIVEYMPVPEGDETAPLQLLVSSIDYNEY
                     VGRISIGRIERGTLSQGQDVMVANYHNVTQPYKAKVTNIYQFEGLKREVVQTATVGDI
                     VSFSGIEDVTIGDTICAVDCVEPLKFVKISEPTMEMTFSVNNSPFAGREGKFVTSRHL
                     RSRLFKELLKDVSLRVEETDSSEAFRVSGRGEMHLSILIETMRREGFEFQVSTPKVLN
                     RVIDGVECEPIEELVIDVPEDCVGSVMEKMGARKADLQHMEPIGNRMRIEFLIPSRGL
                     FGYRSEFMTDTKGDGIMNSIFHGYEPLKGEIPRRMTGSLIAFETGEAVAYGLFNAQDR
                     GVLFINPGTKVYAGMIVGSSPKGDDMAVNVCKKKHLTNTRASGSDDSLRLVPPRNLSL
                     EEALEFIAEDELVEVTPENFRLRKRILDHSMRMRSNAKK"
     gene            3502..4020
                     /locus_tag="VHR42_03080"
     CDS             3502..4020
                     /locus_tag="VHR42_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX3026196.1"
                     /translation="MDHDDRNKEISLKLIRSFMRFKRLHHFGEDDYKRKDSNHIRHSE
                     MSLIFAIRDAEKSYPDGINVKDLSNFLHVKPPSIIPLINSLEKKDMVERSIDRNDRRI
                     IRIRLKDAGYEIIEKSKNHFLARMQGLVDYLGPEKSEQLSDLLQEVYNYVAQLDKCGP
                     ARQEKKKPDIDR"
     gene            4049..>4438
                     /locus_tag="VHR42_03085"
     CDS             4049..>4438
                     /locus_tag="VHR42_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX3026197.1"
                     /translation="MLKIYKNLKPYSLIIAVILVLIFLQVSSDLYLPTLMSDIVNKGI
                     MGEDVSYIFKTGGLMLLVAAGGVVCAIFASFLGSRTAVGLGRILRNKVFNRVENFSLH
                     EFDKFGASTLMTRTTNDIVQIQTVTILI"
BASE COUNT         1203 a         1137 c          905 g         1193 t
ORIGIN      
        1 tccctgaatc cgccgccgca gcttgtgaac attcgtggca cagacgccgg tttcaaggat
       61 tagcctgcat ttgacagcac atcccgtttt cagcaaaaac aaaacgcccc cgctttcacg
      121 gagacgtaat gaattgtgtc cgacttctgg agaccgtttg gcggacggtt ctgaagtaag
      181 ggctttttac cttatatttt ctttgaatta gtccaaacaa ctattttata taacacagta
      241 ctcagacatt cgcattgcgc atgctcgaaa gaataattaa ccataagccc tatctcccca
      301 gttcaaaaca ttctaccctt tttggcaatt gagtttcaga aacatattga cgtgagggca
      361 gccctcgtca aaatagcagt cattttccga caaaaagccg agtttccgat aaaacggctc
      421 cgcctgcttc tgcgcgccaa gcactatctt ttccgctccc agctgctccg ctttgcggac
      481 aacctctttc atcagagccg ctcccaaatg gctgccccga taatctttca gaacggctac
      541 cctgccgaca tgaaagaccc tgccttgttc ggaaaaaagc cgtgccgttg ccacaggtac
      601 accatccaga tacaggacaa catgtaaagc ctcatgatca gtctggtcaa attcatgttc
      661 aaaaccctgc tctttgacaa aaactttttc acgaatttca tgggcatcct gaaaaaactc
      721 cagccccttt gtccatttga actccatatc gcaccatcct tgatttaata gatccaaata
      781 ggtcaggcca aaaatgccaa tcccctgctc acccgaaatc agtcaagaag caacgttgta
      841 acaccgggat tcaaactaat gattttttgt ttaatccttc tgtgtttgag agaacggcga
      901 accatatcac gcaaaaccgt cccggatgca tagccgtgaa tcactatgat ttcttttaca
      961 tttccgttgg cattcgaaag aaaacgctcc agaaatgttt ttgcctccgc ccgtttcata
     1021 ttatgaatat caatttctac cttttgagaa gttatataat ttaccatatt ttatctctta
     1081 tttctttatt ggttttttgt tcgcatgcag acaataacgg cgggctgctg atcataaaaa
     1141 cgagggtttg ctttttattt tataacaata cctggcaact ttcaacccat ttgccaataa
     1201 aacggcaaaa caacaggccc ggcggaaact atccgccggg cctgtgacta tttcaaaatt
     1261 tacttcttgg cgttggatct cattctcata ctgtggtcca gaattctctt gcgaagtctg
     1321 aaattctccg gtgttacttc caccagttcg tcttccgcaa taaattccag cgcctcttcc
     1381 aggctgagat tacgcggcgg caccagcctg agtgaatcgt cactcccaga agcacgcgta
     1441 ttggtcagat gttttttctt acagacgttg accgccatat cgtctccctt cggagagctg
     1501 ccgacaatca ttccggcgta aacctttgtt cccggattta taaacagaac cccgcgatcc
     1561 tgcgcgttaa acagaccgta agcaaccgct tcgcctgtct caaacgcaat cagcgaacct
     1621 gtcatgcggc gcgggatctc gcctttaagc ggctcgtagc catgaaaaat cgaattcatg
     1681 attccgtcgc cctttgtgtc ggtcataaat tcactgcgat aaccaaaaag ccctctggac
     1741 ggaatgagaa actcaattct catccggttt cctatcggtt ccatatgctg cagatccgcc
     1801 ttgcgggcgc ccattttttc cataaccgaa ccaacgcagt cttccggaac atcaataacg
     1861 agttcctcga tcggctcgca ttccactccg tcgatcaccc gattaagaac cttcggcgtg
     1921 cttacctgaa actcaaagcc ctcgcggcgc atggtctcaa ttaaaatcga caggtgcatc
     1981 tcgccgcggc ccgatacacg gaatgcctcg gacgagtcgg tttcctcaac acgaagagaa
     2041 acatctttga gcagctcttt aaacagtctg gaccgcagat gtctggaggt cacaaacttg
     2101 ccctcacggc ctgcaaacgg gctgttgttg actgaaaatg tcatttccat ggtcggctcg
     2161 gagattttta caaatttgag cggctccaca caatccaccg cacagatggt atcgccgatg
     2221 gtgacgtcct cgatgccgga gaagctaacg atgtccccca ccgtagccgt ctgcaccact
     2281 tcacgcttaa ggccctcaaa ctgatagata ttggtaacct tcgctttata gggctgcgtg
     2341 acattgtggt aattggccac cataacatcc tggccctgac ttaacgtacc cctttcgatt
     2401 ctgccgatcg agatccttcc gacatactca ttatagtcaa tagaagaaac cagcagctga
     2461 agcggggctg tctcatcccc ctcgggaacc ggcatatatt cgacgatttt atcaaataac
     2521 ggctttaaat cggtgccctc ctcgtcgggt ttcagcgacg ctgtaccctg acggccggag
     2581 caatagacaa aggggctgtc caactgctcc tcattggcat taaggtcaag gaaaagctcc
     2641 agaacttcgt tttccacttc ccggattctg gaatcaggct tgtcaatctt attgacgaca
     2701 acgataactt tgtgcccaag agccagcgct ttctgcaaaa caaaacgggt ttgcggcata
     2761 ggcccttcgg ccgcgtcaac taaaagcaga accccattca ccatttttag aatgcgctca
     2821 acctcgccgc caaagtcggc gtggccgggc gtgtcaacga tattaatttt gataccatta
     2881 taagtagcgg cggtgttttt ggcgagaatc gtgatgcctc gttctctttc aaggtcattg
     2941 gaatccatga ccctttcctg aacatactga ttctccctga aaacgcctcc ctgcttaagc
     3001 atttcatcga ccaatgtggt ttttccatgg tcaacgtgag cgattattgc gatattcctg
     3061 aggtcgctgc gtaccaatcg aactactcct ctcaaaactc aacaaagtga tgcaaattct
     3121 ttgtcctctc gattcccggc gcgcggcaag cgaataaacg cggtctgctc cgaaaagcga
     3181 tagaaatctg tatgcggccg ttttacggcc gcatacttgt tgtcattata acttaattag
     3241 tttactccgt ttttatcatt ctgtcaaacg ataagacaaa aacatcatta taaacaaggt
     3301 tttagaagaa agccacacct ttttaagaaa ttttgctatt ctaagttttt ttaataaaat
     3361 attgcacaat aattgttacg ccagaactta attttgcaca aaattaccta ttgaaaacta
     3421 aaaaacgtga gattataatt agttaatcta attaatagga agcctaatga ttcggctgcc
     3481 taataaagtg tggtgaaatg aatggatcat gatgaccgca acaaagaaat ctctctaaaa
     3541 ctgattcgct cgtttatgag atttaaacgt ctgcatcatt ttggagagga cgattataag
     3601 cgaaaagact ctaaccatat ccggcacagc gaaatgtcac tcattttcgc gatcagggat
     3661 gcggaaaaaa gctatcccga cggaatcaac gtaaaagacc tgagcaattt tcttcatgtg
     3721 aaaccgccgt ccatcattcc cctgatcaac tcgctggaaa aaaaggatat ggtcgagcgg
     3781 agcattgacc ggaacgaccg gcggatcatc cgaattcgat taaaggatgc cggctacgaa
     3841 atcattgaga aatcaaaaaa tcactttttg gcccgcatgc aggggctggt cgattatttg
     3901 gggccggaaa aaagtgaaca gctttcagat ttattgcagg aagtctataa ttatgtggct
     3961 cagttagaca agtgcggccc agctagacaa gaaaaaaaga aaccagatat tgaccgataa
     4021 tatcttacat ctccaccgga ggtattttat gcttaaaatt tacaaaaacc tcaagccgta
     4081 cagcttgatc atagcggtta ttctcgtatt aatattcttg caggtctcat ccgacctcta
     4141 tctccccacc ctgatgtccg atattgtcaa caagggaatc atgggagagg atgtcagtta
     4201 catctttaaa accggcgggc ttatgctcct ggtagcggca ggcggcgtcg tctgcgcgat
     4261 ttttgcaagc ttcctgggct ccagaactgc tgtcggtctt ggcagaattc tgagaaacaa
     4321 agtatttaac cgggttgaga acttttcgct gcacgaattt gataaattcg gggcttccac
     4381 actcatgacc cggaccacca atgatattgt tcagattcag acggtcacca ttctaatt
//
LOCUS       DATALY010000104         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_110640,
            whole genome shotgun sequence.
ACCESSION   DATALY010000104 DATALY010000000
VERSION     DATALY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110640"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1140)
                     /gene="leuB"
                     /locus_tag="VHR42_03090"
     CDS             complement(61..1140)
                     /gene="leuB"
                     /locus_tag="VHR42_03090"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX3026198.1"
                     /translation="MTFRIAVIKGDGIGPEIVGEAEKVLIKTGEKFGHSFEFTDVLMG
                     GCAIDKTGVPLPDETVEICKKSDSVLLGAVGGPKWDNMPGSLRPEKGLLGIRSALGLY
                     ANLRPAILYPALIGACPLRRDIVEGGLDVLMVRELTGGIYFGERGRRKGKNGEEAFDT
                     ESYSVMEIERIAKTAFSLAKRRRKKLTSIDKANVLESSRLWRETMHRFAKENPEVEYS
                     DLLVDNAAMQLVRNPGQFDVIVTSNMFGDILSDEASMITGSIGMLPSASLGSGKLGLY
                     EPIHGSAPDIAGQNIANPIATVISAAMMLKYSFGLDEEAKTVENAVSKALEQGYRTAD
                     ISDKNTSPDKKIGTREMGNLIASLL"
     gene            complement(1162..1653)
                     /gene="leuD"
                     /locus_tag="VHR42_03095"
     CDS             complement(1162..1653)
                     /gene="leuD"
                     /locus_tag="VHR42_03095"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3026199.1"
                     /translation="MKLCGSVFKFGDNIDTDVIIPARYLNSSEPKELASHCMEDIDST
                     FVKKVKAGDMIVGGRNFGCGSSREHAPIAIKAAGVSLVIASTFARIFYRNAVNIGLPI
                     LECEEASRKIQDGDEIEADLEAGIIYNRTRNETYKSLPFPEFIQKIIDSNGLLNAVKS
                     KEI"
     gene            complement(1671..>2682)
                     /locus_tag="VHR42_03100"
     CDS             complement(1671..>2682)
                     /locus_tag="VHR42_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX3026200.1"
                     /translation="NFAKKHGITNFFDVGCMGVEHALLPEKGLVVPGDLVIGADSHTC
                     TYGAVGAFSSGVGSTDMAAGMVRGICWFKVPAAVRFVISGKPRKWVSGKDVILHIIGL
                     VGVDGMLYKSMEFTGKGLSHLTQEDRLSMANMAIEAGAKNGIFEVDDTTIKYVNEHSQ
                     KPYKIYEADSDAEYEKTYEIDLSKIKPTVAFPHLPENTRTIDECGKIEIDQVVIGSCT
                     NGRIEDLRTAAEILRGRKVADGVRCIIIPATQKVYLDAVNEGLVNVFIEAGCAVSTPT
                     CGPCLGGYMGILADGERAVATTNRNFVGRMGHIRSEVYLAGPAVAAASAVAGRIASPD
                     DI"
BASE COUNT          637 a          732 c          564 g          749 t
ORIGIN      
        1 aataaacagc ggatggatta taaaaaacac aaagccgtat taaaaaagcg gcttttcgta
       61 tcacaataaa gaagcgatta agtttcccat ctctcttgtc ccgattttct tatcgggtga
      121 agtattttta tctgagatat ctgctgtcct gtaaccctgt tccaatgcct ttgacactgc
      181 gttttcaacg gtcttcgctt cctcgtcaag cccgaaggaa tatttcagca tcattgcggc
      241 ggaaatgacc gtcgctatcg gatttgcgat gttctggccg gctatgtccg gcgctgaacc
      301 gtgaattggt tcataaagcc ccagttttcc gctgccgagc gaagcggacg gaagcattcc
      361 gattgaaccc gttatcatgc tggcctcgtc agaaagaata tctccgaaca tattgctggt
      421 tacgattaca tcaaactgcc cggggtttct gacaagctgc atcgccgcgt tgtcaacgag
      481 caaatccgag tattcaactt cggggttttc ttttgcgaac ctgtgcatgg tttcccgcca
      541 cagacgggaa ctttcgagaa cattcgcctt gtcgatgctc gtcagctttt ttctgcggcg
      601 ctttgcaagg ctgaatgcgg tttttgcaat tcgctcgatt tccataacgc tgtagctttc
      661 ggtgtcaaac gcttcttcgc cgtttttacc ttttctgcgt ccgcgttctc cgaaataaat
      721 tccgccggtg agttccctga ccatcaagac atcaaggccg ccctcaacta tgtctcttct
      781 caacggacat gcaccgataa gcgccggata tagaattgcg gggcgcaggt ttgcataaag
      841 cccgagtgcg gaccttatac caagcagtcc cttttcgggg cgcaggcttc ccggcatgtt
      901 atcccatttc ggacctccga cagctccaag cagaacgctg tcgctctttt tgcaaatttc
      961 cactgtttca tcgggcagcg gcactcctgt tttgtcaatc gcgcagccgc ccattaaaac
     1021 atcggtaaat tcaaatgaat gtccgaattt ttcgcctgtt ttaataagta ctttttccgc
     1081 ttcgccgaca atttcagggc ctattccgtc gccttttatc acagcaatcc tgaatgtcat
     1141 taaaatcacc cgccccacaa atcagatttc ttttgatttg acagcattta aaaggccgtt
     1201 gctgtcgata attttttgta taaattcagg gaatggcaga cttttatatg tctcgttcct
     1261 cgtcctgttg taaataatac ctgcttcaag gtcagcttca atctcgtccc cgtcctgtat
     1321 tttccgcgac gcttcctcac attcgagtat cggaagaccg atattaacag cattgcggta
     1381 aaatatacgg gcgaacgtcg aagcgataac aagtgaaacg cctgccgcct ttatagcaat
     1441 cggcgcgtgt tcacgggaag aaccgcaccc aaaattacgc cctccgacta tcatatcgcc
     1501 agctttaact tttttcacaa aagtgctgtc tatatcctcc atgcaatgtg aggcaagttc
     1561 tttcggttca gatgagttaa gataccttgc cggaattata acgtccgtgt ctatattatc
     1621 cccgaactta aatacagaac cgcaaagttt caagctgttc accctctttt ttaaatatcg
     1681 tccggcgatg caatccgccc cgcaaccgcc gaagctgccg cgaccgccgg ccccgcgaga
     1741 tatacttccg atcggatatg tcccatgcgt ccgacaaagt tgcggtttgt tgtggcgacg
     1801 gcgcgttcgc cgtcggccag aatccccata tatccgccga ggcaaggccc gcaggtcgga
     1861 gttgaaaccg cgcaccccgc ttcaatgaaa acgtttacaa gcccttcgtt aacagcatcg
     1921 aggtaaacct tttgagttgc cggaattatg atacagcgaa caccgtcggc aacctttctt
     1981 ccgcgcagaa tttccgccgc cgtccttaag tcctcgatac gtccgttagt gcatgagccg
     2041 attacaacct ggtcaatctc tatcttccca cactcatcaa tcgttcttgt attttccggc
     2101 agatgtggaa aagcaactgt gggtttaatc tttgataggt cgatttcata agttttttca
     2161 tactcagcgt cgctgtctgc ctcatatatc ttataaggct tttgggaatg ttcatttaca
     2221 tacttaatcg ttgtatcgtc aacttcaaat atcccgtttt ttgccccggc ttcaatcgcc
     2281 atattagcca tgctgagcct atcctcctgc gttaagtggg aaaggccttt gcctgtaaat
     2341 tccattgatt tataaagcat gccgtcgacg ccgacaagcc cgatgatatg aagtatcacg
     2401 tccttgcccg atacccattt tcggggtttt ccggaaatga caaacctgac ggccgccgga
     2461 accttgaacc agcagattcc tctcaccatt cccgcggcca tatcagtact cccgacaccc
     2521 gatgagaacg cacccacagc accgtaagta caggtatgcg aatctgcccc tatgacaagg
     2581 tcgcccggaa caacaagtcc cttttcaggc agcagcgcat gttcaacacc catgcagcca
     2641 acatcaaaaa agtttgttat accgtgcttt tttgcgaaat tt
//
LOCUS       DATALY010000105         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_110816,
            whole genome shotgun sequence.
ACCESSION   DATALY010000105 DATALY010000000
VERSION     DATALY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110816"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /gene="ligD"
                     /locus_tag="VHR42_03105"
     CDS             <1..1457
                     /gene="ligD"
                     /locus_tag="VHR42_03105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX3026201.1"
                     /translation="QTSDGVSRFDTSIRTGRSMEEIERGESEKITRNPFQRMDVQLAR
                     LVNTVPEGEDWLFELKYDGYRIIAYLEGDSVRLITRNGNDFTSRFQDVADALADWANG
                     RAMILDGEMAITDAQGRTDFQALQSYMRNPGGKKLTYIVFDLLALDGADLRGRRLLER
                     KDMLETLMKNAPKNLYCSKYIKGNGKESLLAACKLNLEGIVGKKADSVYSGTRNGDWV
                     KLKCEKRQEFVIGGYTRSDKKSGGISSLLLGVYEGTELIYAGRAGTGLTERGMMELEK
                     KFESIKAETAPFKNAPAPKTNESFTWLKPVMAAEIRFAEWTSDHLLRQASFKGLRTDK
                     DPREIKMETACDAAEPEDITNGEKPSYENTERAMKTNGDSIIINGITVTSPDKVIFED
                     PEIRKADVVQYYANISERMMPYAGNRILSIVRCPKGVSQSCFFKKHPGPDSKGIVTLP
                     VTTGSGETEDYFYIESPSGLITEAQMGTLEFHIW"
     gene            complement(1354..2001)
                     /locus_tag="VHR42_03110"
     CDS             complement(1354..2001)
                     /locus_tag="VHR42_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3026202.1"
                     /translation="MNLALWHKIFINFIKDHRYLYLLNGLKVTLIVTFFAVILGIVIG
                     LFVAIVKVAAANNRKLRPLEFIVNIYITVIRGTPVMVQLLIIYFVVFASANVSQVLVA
                     IVAFGINSGAYVSETIRSGIMAVDIGQTEAGRSLGFNSAETYRYIVLPQALKNILPAL
                     GNEFIALLKETAVVGYIGLQDLTRYGIQACPSVLPLSARKGSQYRSSLPFLRCLS"
     gene            complement(2315..3064)
                     /locus_tag="VHR42_03115"
     CDS             complement(2315..3064)
                     /locus_tag="VHR42_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3026203.1"
                     /translation="MKSISKALIITLVLALAGSMAGCAKNVGLKEKGKLIISTNAEFQ
                     PFEYMDNNQIIGIDVDISKQIAKDLGVTLQIDNMNFDSVVTAVQTGKTDMAVAGLTKN
                     SDREEAVDFSDPYYDASQVIIVKSTNTAINSENTLKGKKIAVQKGTTGDDLATKLVGD
                     SKMSRFNASTDAITALKGGKVDAVVIDSFPAKIFVKQNSDLKIVGSPLTSEQYCIGIH
                     KGNKNLVDAVNKTLSKMKSSGQLDEIMKKYS"
     gene            complement(3434..>4052)
                     /gene="ligD"
                     /locus_tag="VHR42_03120"
     CDS             complement(3434..>4052)
                     /gene="ligD"
                     /locus_tag="VHR42_03120"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX3026204.1"
                     /translation="TGSGETEDYFYIESPSGLITEAQMGTLEFHIWGSRADNLEKPDM
                     MVFDLDPDEGMEVEKIRAGVRDIKSVLEQLSLVSFLKTSGGKGYHVVVPFEPSVTWDS
                     FHDFARRIAEFMEQKWPDRYTSNVRKDRRKGKIFIDWMRNGRGATSIAPYSLRARKGA
                     SVSMPISWDELDTVAPDGVDMAGALMRISGRDPWKDFYEIRQRLK"
BASE COUNT         1113 a          957 c          984 g          998 t
ORIGIN      
        1 cgcaaacatc ggacggggtt tcccggtttg acacgagcat cagaaccgga cgcagcatgg
       61 aggagatcga acgcggagag agcgaaaaaa tcacgagaaa tccctttcaa cggatggacg
      121 tgcagcttgc aagacttgtc aataccgttc ctgagggcga ggattggctc tttgagctga
      181 aatacgacgg ctaccggatt atcgcctatc tggagggcga cagcgtccgg cttatcacaa
      241 gaaacggaaa cgattttaca agccggtttc aggatgtcgc cgacgccctc gccgactggg
      301 caaacggcag agcaatgatt ctggacggcg aaatggcgat tacggacgcg cagggcagga
      361 cggattttca ggctctgcaa agctatatgc gcaatcccgg tggaaaaaag ctcacctata
      421 tcgtttttga cctgctggcg ctggacggag ccgacttaag aggccgccgc cttcttgaac
      481 gaaaagatat gcttgaaacg cttatgaaaa atgctccgaa aaatctatat tgcagcaaat
      541 atatcaaagg gaatgggaaa gaaagcctgc ttgcggcctg taaattaaat ctggagggga
      601 ttgtagggaa aaaagccgac tccgtttaca gcggaacaag aaacggcgac tgggtcaagc
      661 ttaaatgtga gaaaaggcag gagttcgtca taggcggata tacgcgttcc gataaaaaat
      721 cgggcggaat cagctcgctt ctgctcggcg tttacgaggg gacggagctg atttatgccg
      781 ggcgcgcggg gacgggcctc acggaacgcg gaatgatgga gctcgagaaa aagtttgaaa
      841 gcatcaaggc tgaaacggct ccatttaaaa acgcgcctgc gccgaagaca aatgaaagct
      901 tcacctggct taagcctgta atggcggctg agatcaggtt tgccgaatgg acctccgatc
      961 atcttctgcg gcaggcaagt ttcaaagggc ttcggacgga taaggaccct agggaaatca
     1021 aaatggaaac ggcgtgtgac gccgccgagc ccgaggatat aacaaacggt gagaaaccgt
     1081 cctatgaaaa tacggagaga gcaatgaaaa caaacggcga ttcgattatc ataaacggaa
     1141 ttacggtcac gagcccagat aaggtgatat ttgaagaccc cgaaatcagg aaagcggacg
     1201 tggttcagta ttacgcaaat atttctgagc gtatgatgcc ctatgcaggc aacaggattc
     1261 tgagcattgt acgatgcccg aagggtgttt cccagtcctg cttctttaag aagcacccgg
     1321 gcccggacag caagggcatc gtcacgctgc cggtcacgac aggcagcgga gaaacggaag
     1381 actacttcta tattgagagc ccttccgggc tgataacgga agcacagatg ggcacgcttg
     1441 aattccatat ctggtaagat cctggaggcc aatgtacccg acgacagcgg tttcctttag
     1501 cagggcgata aattcattgc cgagagcggg aagaatattt ttcaaggctt gcggcagcac
     1561 gatataacgg taggtttccg ccgagttgaa gccaagcgac ctgcctgcct cggtctgccc
     1621 gatatcaacc gccatgattc cagaacggat tgtttcagag acatatgcgc cggaattaat
     1681 gccgaatgcc acaatggcga ccagcacctg cgaaacattg gcgcttgcaa acacaacgaa
     1741 atagataatg agcagctgta ccatgaccgg cgtgccccgg ataaccgtga tgtagatgtt
     1801 cacgataaat tccagcgggc ggagctttcg gttgttggca gccgccacct taacgattgc
     1861 gacaaacagc ccgatcacaa tgccgaggat aaccgcgaaa aacgttacga taagcgtaac
     1921 ctttagcccg ttgagcagat acaaatagcg atgatcttta ataaaattta taaagatttt
     1981 atgccataag gccaaattca tcacaatcct tccgaggtac tactggatac aactgaatca
     2041 ataattaata taggagcagt tgtatttagg gaacggctaa acgctaaagc acgcgcgaaa
     2101 ttttgctacg caaacgcgca tgtgcaatat atataacata atggccaagt cctgtcggca
     2161 gatatactaa aaacttattc tgttatataa atgcggtcag aaatagaaac tggatatagg
     2221 cacattgctg ccggaagatt aatattacac agcaatagca gccgttaacc ggctgctatg
     2281 caatcgctct gtgcaatttt acctaattat cggctcagga atattttttc atgatttcgt
     2341 cgagctgacc gctgcttttc atcttgctca gcgttttatt cacggcgtcc acaagattct
     2401 tattgccttt atgtatgccg atgcagtact gttccgacgt cagcggcgaa cccacaattt
     2461 taaggtcgga gttttgtttg acaaatattt ttgccgggaa gctgtcaatc acaacggcgt
     2521 ctacttttcc acctttcaga gcggtgatgg cgtccgtcga tgcattgaaa cgggacattt
     2581 tcgagtctcc gacaagcttt gtcgcaagat cgtcgcccgt ggtgcctttt tgaaccgcta
     2641 tttttttgcc tttcagcgta ttctcgctgt taatggctgt gttggtgctt tttacgataa
     2701 tgacctgaga cgcgtcataa taaggatcgg aaaaatcgac ggcttcttcg cggtcagaat
     2761 ttttggtaag cccggcaacc gccatatccg ttttgccggt ctgcacggcg gtgacaaccg
     2821 agtcaaaatt catattgtca atctgaagtg tgacgcccaa atcttttgcg atctgtttac
     2881 tgatatcaac gtcgatacct ataatctgat tgttgtccat gtactcaaac ggctgaaatt
     2941 ctgcgtttgt cgagataatt agtttgcctt tttcctttaa gccgacattt ttggcacagc
     3001 ccgccatcga tccggccaga gcgagaacta atgtaataat aagtgctttc gatatgcttt
     3061 tcacgaaaca gacccctctt cctgtatcaa taattcatac ataattaatt attatacata
     3121 gtgcacggca aaaatgcaag aatattcgct taaattccaa aataataatg aaaaatgtat
     3181 atcattattt atttaaaata aattataaca aaacagggat gatgatacat cccactataa
     3241 atctttgaaa gggaaaaccg ggctgccttc gatcaagaac tacggaatct gtaactttcc
     3301 ttattatatg aattcatttt catattgtgt tttcatgcat ttgtcctgaa tgatgcggag
     3361 cagggaatac agctgtcaaa acaaggacaa gccctcattt tctgagggct tgtaaactcc
     3421 ggcttcaata aagctatttc agtcgctgcc tgatctcata gaagtctttc cagggatctc
     3481 tgccggaaat tctcatcagc gcgcccgcca tatcgacccc gtcgggcgcc acggtatcaa
     3541 gctcgtccca ggaaatcggc atcgaaacgg aagccccttt tctcgcccgc agggaatagg
     3601 gggcaatgct tgtggcgccc cggccgttgc gcatccaatc gatgaagatt ttacctttac
     3661 gcctgtcttt ccgaacattg cttgtgtagc ggtcgggcca cttttgttcc atgaattcgg
     3721 caatgcgcct tgcaaaatcg tgaaaactat cccatgttac ggacggctcg aaaggcacga
     3781 ccacatgata ccccttgccg ccgctggttt taaggaatga gaccagggaa agctgctcaa
     3841 gtacgctttt gatgtccctg acacccgcac gtatcttttc aacctccatt ccttcatccg
     3901 ggtcgaggtc aaaaaccatc atatcgggct tttcgaggtt atccgcgcgg cttccccaga
     3961 tatggaattc aagcgtgccc atctgtgctt ccgttatcag cccggaaggg ctctcaatat
     4021 agaagtagtc ttccgtttct ccgctgcctg tc
//
LOCUS       DATALY010000106         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_197733,
            whole genome shotgun sequence.
ACCESSION   DATALY010000106 DATALY010000000
VERSION     DATALY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197733"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..201
                     /locus_tag="VHR42_03125"
     CDS             16..201
                     /locus_tag="VHR42_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HEX3026205.1"
                     /translation="MSVKKMNQPNRGIRCLVNTCHYYMQGDHCAAEKIEVEPKNAADT
                     QETDCSTFLPENSQRNM"
     misc_binding    376..634
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            692..2362
                     /gene="ilvB"
                     /locus_tag="VHR42_03130"
     CDS             692..2362
                     /gene="ilvB"
                     /locus_tag="VHR42_03130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX3026206.1"
                     /translation="MKMTGAEILVNCLLEQGVDTVFGYPGGAVLNLYDALYQYSGQIT
                     HIRTCHEQAAAHAADGYARSTGRTGVVIATSGPGATNLVTGIATAYMDSVPLVAITGN
                     VARHLLGKDSFQEVNITGITRPVTKRNYMVAEVEDLAAIIREAFQIAASGRPGPVLID
                     IPKDVTAAEAEYQRREPEAIQPVTEMICDEATAEAVRLIQNCERPFVFAGGGVIASGS
                     SGELFEFSRRAGAPVALSLTGLGAFPADDEYFTGMIGMHGSRASSLAVTDCDLLIAAG
                     VRFSDRVLCSPEKFAPNAKVIHIEVDPKEINKNIRVDCALTGDLREVLKRLNARLKPA
                     DRPEWLGRVGDWKRQLPIEMPERDAVTPWQVIRTLRELTGGEAIITTEVGQHQIWVGQ
                     YYGFNTPRSFVTSGGLGTMGFGLGAAIGAQAGNPGKRVVNIAGDGSFHMNLQELTTAV
                     TYHLPVIELVMNNGVLGMVRQWQKIFYEGRLSQTTLQRQTDYIKLAEAFGARGFVIDS
                     KDAVVPVLKTALEANQPCVIDCRIDRDFNVLPMVPAGAPINEPILEME"
     gene            2408..>2845
                     /locus_tag="VHR42_03135"
     CDS             2408..>2845
                     /locus_tag="VHR42_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX3026207.1"
                     /translation="MTGSKKIEIFDSTLRDGAQGEGITFSVEDKLNIVKTLDRLGIAY
                     IEAGNPGSNPKDLDFFSRASRLTLGNAKLCAFGSTRRAGIKIEEDRSVAALLGANTGT
                     VAIFGKAWDLHVTKVIKTTLEENLRMIEETVAFFKSHGREVVFD"
BASE COUNT          731 a          648 c          854 g          612 t
ORIGIN      
        1 cgggaggaaa gatcaatgtc cgttaaaaaa atgaatcagc ccaatcgtgg aatcagatgt
       61 cttgtgaata cctgccatta ttacatgcag ggcgaccact gcgcggcaga aaaaattgaa
      121 gttgagccga agaatgcggc cgatacccag gaaaccgact gctcgacctt tttgccggaa
      181 aacagtcaga ggaatatgtg atagtaggcg taacaaacaa tagcttaaaa agctgccgaa
      241 aaaacggcag ctttttagtt agcaacccat tattgtttat cggcgtcctt cttataatca
      301 ttagagcaaa acaataaaaa atttgcagtg gacagttgac aaaagaaaga cggatttata
      361 taataaaaca aacgaataaa tgcgatgatg gggacagtaa cagtctatcg agcttttcag
      421 agagctgccg gcaggtgtga ggcagcaggc ccggctgtga aacatcaccc ttgagcggat
      481 caccgaaatg gtattccaac cataatagga tgatacggcc gcccgccgtt atacgggaac
      541 gctgttggcg cgcaaagcaa gacagtggtg agaggacgtc tgtttcaggc gtcaatgaga
      601 gtggtaacgc ggagatttgg tcttcgtctc ttaggaggcg aaggcctttt tgtatattcg
      661 gatcattttt cgggaaaagg aggccgctat catgaaaatg acaggagcgg aaattctggt
      721 aaactgcctt ctggaacagg gtgttgatac tgttttcgga taccccggag gggcggtgct
      781 caatctctat gacgcccttt atcaatacag cggtcaaatc acgcatattc ggacctgtca
      841 tgagcaggcg gctgcacacg cggcggacgg atatgcgaga tcaaccggga gaaccggcgt
      901 ggtaattgcc acttccgggc ccggcgcgac caatcttgtg accggaatcg ccacggcgta
      961 tatggattcg gtgccgctgg ttgccattac gggaaacgtg gcgagacatc tgctgggaaa
     1021 agacagcttc caggaggtca atatcaccgg tatcacaagg ccggtgacca aaagaaatta
     1081 tatggtcgcc gaagtcgaag atctcgccgc gattatccgg gaggcttttc agattgcggc
     1141 aagcgggagg ccggggcctg ttctgattga tattcccaaa gacgtcacag cggccgaggc
     1201 ggaatatcag cggcgtgaac cggaagccat ccaacctgta acggaaatga tctgcgatga
     1261 ggcgacggca gaagcggttc ggctgattca aaactgtgaa aggccgtttg tgtttgccgg
     1321 aggcggcgta attgcatccg gatcgagcgg cgaactgttt gagttttccc gccgggcggg
     1381 cgcgccggtg gcgctgagcc tgaccggtct gggggcgttc ccggccgatg acgagtattt
     1441 taccggcatg atcgggatgc acggcagcag ggcgtcgtcg ctggcggtta cagactgcga
     1501 tctcctgatt gcagcgggcg tccgtttttc cgaccgggta ctctgcagtc ctgaaaaatt
     1561 cgctccaaat gccaaagtca ttcatattga agtcgaccca aaagaaatca acaagaatat
     1621 ccgggttgac tgcgcgctga ccggtgatct gagagaggtt ctgaaacggc ttaacgcgag
     1681 gctgaaaccg gctgaccggc cggagtggct ggggcgggtc ggagactgga aaagacagct
     1741 tccgattgag atgccggagc gggatgccgt gacgccgtgg caggtgatcc ggacgctgcg
     1801 ggagctgacc ggcggcgagg ccattattac caccgaggtc gggcagcatc agatatgggt
     1861 cgggcagtat tacggtttta acaccccacg gagttttgtc acctcaggcg ggctgggaac
     1921 aatgggcttc ggtcttggag ccgcgatcgg cgcgcaggcc ggaaatcccg gcaagagggt
     1981 ggtcaatatc gccggcgacg gaagctttca tatgaacctg caggagctga caacggcagt
     2041 gacctatcac ctgcccgtca ttgagcttgt gatgaacaac ggcgttctcg gcatggtccg
     2101 ccagtggcag aagatttttt acgagggacg gttgtcccaa acgacacttc aaaggcagac
     2161 cgattacatc aaacttgcgg aagcgtttgg agcgcgggga tttgtgattg acagcaaaga
     2221 tgcggttgtt cctgttttaa agacggcgct ggaagccaat cagccctgtg tgatcgactg
     2281 cagaatcgac agggatttca atgttcttcc gatggttccg gcgggcgccc cgattaacga
     2341 accgattttg gaaatggaat gacagacggc agtttgtttt taaaaatatc tgatgaggag
     2401 gatggctatg acgggcagca aaaagatcga aatctttgat tccacgttaa gagacggtgc
     2461 gcagggcgag ggaatcactt tttcagtgga ggataaactc aatattgtca aaacactcga
     2521 caggcttgga attgcgtata ttgaagcggg gaaccccggc tccaacccca aggatctgga
     2581 ctttttttca agggcttcca ggcttacgct ggggaatgca aagctttgcg cgtttggaag
     2641 cacccgcagg gccggaatta aaatcgaaga agaccgcagc gtcgccgccc ttctaggggc
     2701 gaataccgga accgttgcga ttttcggcaa ggcgtgggat cttcatgtga cgaaagtcat
     2761 caagaccaca ttggaagaaa atctgaggat gattgaagag acggtggcat tttttaaaag
     2821 tcacggcagg gaagtggtgt ttgac
//
LOCUS       DATALY010000107         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_800305,
            whole genome shotgun sequence.
ACCESSION   DATALY010000107 DATALY010000000
VERSION     DATALY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800305"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VHR42_03140"
     CDS             <1..439
                     /locus_tag="VHR42_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEX3026208.1"
                     /translation="VSPDFLKLRGRIITILQEESKLMEIVKLIGSDTLPDYQKLVLET
                     AHAIRIGFLQQDALDDRDTYVPIEKQKKMMETFDLYYSKAKELIAAGIPLSTISQSGL
                     YEKLIKMKVRIPNGKPELFDDLMEEICNTLDSLAPSKSGTKKP"
     gene            complement(690..1970)
                     /gene="serS"
                     /locus_tag="VHR42_03145"
     CDS             complement(690..1970)
                     /gene="serS"
                     /locus_tag="VHR42_03145"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3026209.1"
                     /translation="MLDIKLLRTDPELVKENIKKKFQDEKIPLVDEVVEMDRKFREAK
                     SRCDELRGQRNTVSKEIGGLMAKGMKEEAEKRKAGVAAIAKEIAGLEQLAQELSAKIR
                     ERMYTIPNIIDESVPIGKDDSENVEIERFGEPVVPDFEVPYHVDIMERFHGIDLDSAR
                     KTSGSGFYYLCGDIARLHSAILSYARDFMIDRGFTYYIPPFMIHGEVVKDVMSFSEME
                     NMMYKIEGEDLYLIGTSEHSMIGKFIDTILQEDQLPQTLTSYSPCFRKEVGAHGIEER
                     GVYRIHQFEKQEMVVVCKPEDSMDWFAKLYHNTVDFFRSLEIPVRTLECCSGDLADLK
                     VKSIDVEAWSPRQKKYFEVGSCSNLGDAQARRLGIRVKSKEKGNYYPHTLNNTVVAPP
                     RMLIAFLENNLNRDGSVTLPEPLRMYMGGKKEIR"
BASE COUNT          591 a          617 c          506 g          589 t
ORIGIN      
        1 cgtttctccc gattttttga aactccgcgg caggataatc acaatccttc aggaagaaag
       61 caaactgatg gaaatcgtaa agctgatcgg ttcggacacg cttccggatt atcagaagct
      121 agtgctggaa acggcgcatg ccatccgcat cggatttctg caacaggacg cgcttgacga
      181 tcgtgataca tatgtcccga tcgaaaagca gaaaaagatg atggaaacgt ttgatctcta
      241 ctacagcaag gcaaaagaat tgattgctgc gggcattccg ctgtcgacga tctcccaaag
      301 cggcctgtat gaaaaactga taaaaatgaa agtccggatt ccgaacggca agccggaact
      361 gtttgatgac ctgatggagg aaatctgcaa cacgctggat tcccttgcgc caagcaaaag
      421 cggcacaaag aagccgtaag agccgaaccg gttcttcccc ctgcgtgtta tttccggacg
      481 ccggagggtt ccggcacccg gttttcatta aaccagttgc aaaataaaac acccctgtcc
      541 ggtgaatact gtagagtatt caccggacag gggtgtttct cagtgatgcg gccgggaaac
      601 aggatatccc gccgccgtca aatccctctg agggcactct gcgcggctcc cgcggatcag
      661 tgaaaaaacg gcaatcaatc tacggttttt taccggattt ccttttttcc gcccatgtac
      721 atccgcagag gctccggtaa ggtgacggaa ccatcccggt tcagattatt ctccagaaag
      781 gcaatgagca tgcgcggagg cgcgacgact gtgttgttca gcgtatgtgg atagtaattt
      841 cccttttcct tactcttcac ccggatgcca aggcggcggg cctgagcgtc tccaaggttg
      901 gaacagctgc ccacttcaaa gtactttttc tggcggggcg accaggcttc gacatcaatg
      961 ctcttgacct ttaaatcggc cagatccccc gaacagcatt ccagcgtgcg aaccggaatc
     1021 tcaagggacc ggaaaaaatc caccgtgtta tgataaagtt tcgcaaacca gtccatgctg
     1081 tcttccggct tacagacaac caccatttcc tgcttttcaa actgatgaat ccggtagacg
     1141 ccccgttctt ctattccgtg ggctccgact tccttcctga aacaaggcga ataactggta
     1201 agtgtctggg gaagctgatc ctcctgcagg atcgtatcga taaacttgcc gatcatggaa
     1261 tgttcgctgg tgccgatcag gtacaggtcc tccccttcaa ttttatacat catgttttcc
     1321 atttccgaaa agctcataac atccttcacc acttctccgt ggatcataaa gggaggaata
     1381 taataggtaa agcccctgtc aatcataaaa tctctggcat aggaaagaat cgcagaatgc
     1441 aggcgcgcga tatccccgca caggtaataa aagccgctgc cgctggtttt tctcgcgctg
     1501 tcgagatcaa ttccatggaa ccgttccata atatcgacat ggtaaggaac ttcaaaatca
     1561 ggaacgacag gttccccgaa gcgctcaatt tccacatttt cgctgtcgtc tttcccgatc
     1621 ggaacagatt cgtcaataat gtttgggatg gtatacatcc gttcccgaat tttggccgac
     1681 agttcctgcg cgagctgctc gagtccggca atctcttttg cgattgcggc gacgcccgct
     1741 tttctcttct cggcttcctc tttcatgccc tttgccatca agccgccgat ttccttgctg
     1801 acggtgttgc gctggcctct taattcatcg catctggatt tcgcttccct gaattttcga
     1861 tccatttcca caacttcatc aaccaaagga atcttttcat cctgaaactt ttttttgatg
     1921 ttctctttta caagttccgg gtccgttctc agtagtttaa tatcaagcat aaaatttccc
     1981 ccataaaaat atttatctgc tttcctgtaa taaaaaaaca aaacccttcc ccccataaac
     2041 catgggacgg aaggattccg cgttgccacc caaattgccg actgccgtcg gccgctcaaa
     2101 cgtgcgataa agggcacgaa ccccgaaaga taaaatcagc tcagaaagtg gaacaaacca
     2161 gccttccgcc ggctcgcacc agtcaccggc tctcttaagg aaacagcagg ttatagcttt
     2221 cctcacagct gtctttattt cagttatgct cttatactat acataaactg gataatcata
     2281 cagacaagaa aacatttttt ttc
//
LOCUS       DATALY010000108         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_370014,
            whole genome shotgun sequence.
ACCESSION   DATALY010000108 DATALY010000000
VERSION     DATALY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370014"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VHR42_03150"
     CDS             complement(<1..322)
                     /locus_tag="VHR42_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026210.1"
                     /translation="MEKNDHVRTEGAPLYLGILSGILLLISTILQSGVTKAYTNSLLA
                     STGLTMGQAYFTSISVIGYVIYPGIFLILLLMAIKKPKRGTAFSIVWIILSGLSVIFG
                     IRSIF"
     gene            745..>2323
                     /locus_tag="VHR42_03155"
     CDS             745..>2323
                     /locus_tag="VHR42_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX3026211.1"
                     /translation="MQEQLNYPMSAREKKNAELSREAATNGIVLLENRNGTLPLKTKV
                     RQIALFGCGAVRTVRGGTGSGDPLNGGISGGGNIHVNQSPRYHINIMSCFLAAGYRVV
                     TEDLLNEYAIRYDAEEEKWAGNPLATFAFPELRYSPEQISRYAGMTDTAVYVLSRNSG
                     EGADRCLKKNVTIDGKEYEIGDYDLSRTELENIEAISGTFSKMILVLNVGGVVDMSAI
                     QKNQKIGAVLLMSQAGQEGGNALLDVLTGKVTPSGKLTSTWAKTYSDYPASATFAYND
                     NDVSKEKYEEGIYVGYRYFDTFGITPCYEFGYGLSYTDFSMECRGAEIKGEWLNLKVN
                     VTNEGNVYSGREVVQVYYSAPGGWNEIEGVEKPFQELASFAKTKELRPGESQTLSIRF
                     RIREMASYCEKHACYQLDRGDYRIRIGNSSKHTVPAVLLKLEKAVVTEQLRNEYPLSE
                     KLHEISKAGRTPCRNESWNDGSVVVLTCDGNGIAARNQELPLNTEEVVTYTTDPSYKA
                     TMPYEKVKVVEKKNITLK"
BASE COUNT          708 a          503 c          554 g          558 t
ORIGIN      
        1 taaaaatact tcttattcca aaaataacgc tgagccctga aaggatgatc caaacaatgg
       61 aaaaagcagt tcccctcttc ggtttcttga ttgccatcag cagaaggatc aggaaaatac
      121 caggatagat cacatatcct atcaccgaaa ttgatgtaaa atacgcctga cccatcgtca
      181 accctgtgga tgcgagaagt gaatttgtat atgcctttgt tacacccgac tgaaggattg
      241 tactgatcag cagcaaaatt cctgagagga ttcccaaata aagcggtgct ccttcagttc
      301 taacatggtc attcttttcc ataacgctct cttctttcta cttttttatc tctggatgtt
      361 caagattaca aacatccttg atcagccgcc tttgtgaaca ggattcaaaa tgatcataat
      421 atagtcgaaa tcgtttttat tatattcttc cgttcattat aattcaagca gttatatttg
      481 cgaattcgac taagacaagt gcgctcttga ttaagcaata tagcaacaag tttctccttg
      541 taaggactgt taatgggcga caaaaatcca cataacagac ggatacaaaa gaaatatgta
      601 ttctcctaaa ctgtcggagg aagaatattt gactcattca atagatgcat aatgatattg
      661 attgtgccca ttcagccaca ccgcttataa aattgcaata acactttccg ggaccggaaa
      721 tatagaaaca ggaggagcaa taaaatgcag gaacaactga actacccaat gtccgcaaga
      781 gaaaagaaaa acgcagagct ttcacgggaa gccgcgacaa acggtatagt gcttctggag
      841 aacaggaacg gaacgctgcc actgaagaca aaggtcaggc agattgccct gttcggctgt
      901 ggtgcggtac gcacggtgag gggaggaacc ggctcgggtg acccgcttaa cgggggaatt
      961 tcgggcgggg gcaacattca tgtgaaccaa agcccccggt atcatatcaa catcatgagt
     1021 tgttttctgg cggctgggta tcgcgttgtt acggaagacc tgctgaacga atatgccatc
     1081 cgatacgacg cggaagaaga aaaatgggca ggcaatccgt tggcgacatt tgctttcccg
     1141 gagctgcgct attccccgga acagatttcc aggtatgccg gaatgaccga caccgccgtg
     1201 tatgttttgt ccagaaattc cggagaaggc gcggaccgct gcttgaaaaa gaacgtcacc
     1261 atcgatggca aggaatatga gattggagac tatgatctca gccggaccga actcgaaaac
     1321 atagaagcaa tatccggtac attttcaaaa atgattcttg ttctgaacgt cggcggagtt
     1381 gtagatatga gcgccattca gaaaaatcaa aaaatcggag cagtactcct catgtcacag
     1441 gcgggtcagg agggcggcaa cgcgctgctg gatgtgctca caggaaaagt aacgccatcc
     1501 ggcaagctta cctcgacttg ggcaaaaact tactccgatt accccgcgtc ggccactttt
     1561 gcttacaatg ataacgacgt cagcaaagaa aaatatgagg aaggaattta tgtaggatac
     1621 cggtatttcg ataccttcgg tattactcca tgctatgagt ttggctatgg cctttcttat
     1681 accgatttct ccatggaatg ccgtggcgcc gaaattaagg gagaatggct caatcttaag
     1741 gtaaacgtga caaatgaagg aaacgtttat tccggcagag aagtggtgca ggtgtactac
     1801 tccgcacccg gcggctggaa tgaaattgag ggggtagaaa agccgtttca ggaactggct
     1861 tcttttgcga aaacgaagga actcagaccc ggagaaagtc aaacactttc cattcgattc
     1921 cgaatccgtg aaatggcttc ctactgcgaa aaacacgcct gttatcagct ggaccggggt
     1981 gattatagaa tccgaatcgg caattcgtca aagcatacgg ttccggctgt tctgctgaag
     2041 ctggaaaaag cggttgtgac ggaacaactg cgcaatgaat atccgttatc tgaaaaactg
     2101 catgaaatca gtaaagctgg caggactccc tgccgaaatg agagctggaa tgatgggtcc
     2161 gttgttgttc tcacgtgcga tggaaacgga attgctgcaa gaaatcagga attgcctttg
     2221 aatacggaag aagtcgttac ttacacgaca gaccccagtt acaaggcgac gatgccctat
     2281 gaaaaagtga aagtcgttga aaaaaagaat atcactctga aag
//
LOCUS       DATALY010000109         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_457649,
            whole genome shotgun sequence.
ACCESSION   DATALY010000109 DATALY010000000
VERSION     DATALY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457649"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHR42_03160"
     CDS             <1..489
                     /locus_tag="VHR42_03160"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX3026212.1"
                     /translation="IHERGGKLSAGFAVRMPGNYIASYGAFPQRLQKLLFKRSKRKVL
                     AITTEIVMKKVKRIPRGSVLMRASQTASHKILDSFGEKARNFRVNGKCIGCAICCRIC
                     PDGNIKINEGKPIWGSICEQCMACIQWCPYKSIEYSDKTEKRSRYRHPEVNVTDIVSK
                     RS"
     gene            complement(620..706)
                     /locus_tag="VHR42_03165"
                     /pseudo
     CDS             complement(620..706)
                     /locus_tag="VHR42_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014601873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(703..1056)
                     /locus_tag="VHR42_03170"
     CDS             complement(703..1056)
                     /locus_tag="VHR42_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HEX3026213.1"
                     /translation="MLELIKKMIGDKKEYREQMARVKALPEDYRFVFNKIQEYMWNFA
                     GGDGSDMLKTQYELIELFESGAANGRHVLDVTGEDVVGFCDELLHDTKKWTDNYRKKL
                     NRDMMNKFGRENDSK"
     gene            complement(1059..1430)
                     /locus_tag="VHR42_03175"
     CDS             complement(1059..1430)
                     /locus_tag="VHR42_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HEX3026214.1"
                     /translation="MLDSIIKLIIGDLEEKRAYKQMMKRVDALPKDFRFAFRKIQHYM
                     FTVGPPDGDLTIFTDLTLFTDLVDLFEASAAEGRQVLDVIGSDVGKFSDEFMRAEVPN
                     TEKHREKLNKEILEKFNKEGQ"
     gene            complement(1433..1759)
                     /locus_tag="VHR42_03180"
     CDS             complement(1433..1759)
                     /locus_tag="VHR42_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX3026215.1"
                     /translation="MLENLTEMLKGVLEGCVLEIISHKETYGYEITRRLNALGFTDVV
                     EGTVYTILIRLEKNKLVEITKKPSDMGPPRKFFALNNAGREELRRFWEKWEFVASKIN
                     QLKEEP"
     gene            1969..2907
                     /locus_tag="VHR42_03185"
     CDS             1969..2907
                     /locus_tag="VHR42_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026216.1"
                     /translation="MENIMTVKNLTKSFKDNKVLKGISFDVLRGEVLCILGPNGAGKS
                     TTINILTAALGSDGGEINFKGKKIRRHLRAYKQVLGIVPQDIALYDELSAERNLRFFA
                     SLYGLHGDALTNAVNEALIFSGLDDRRHDKVKTFSGGMKRRLNIACAIAHHPELVIMD
                     EPTVGIDPQSRNHILDSIKKLRDSGMTVIYTTHYMEEVEAISSRIIIMDHGAIIAEGT
                     KETLKEDIANEKRYAIQVEENGKIPAEEFYSIEGVKSAKIDGDSIEITSLKGIENLDH
                     IIALLCDRGEKIRNITSETASLEMVFLKLTGRKLRD"
     gene            2922..3662
                     /locus_tag="VHR42_03190"
     CDS             2922..3662
                     /locus_tag="VHR42_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026217.1"
                     /translation="MRRFFSIFKMDMINLFKNPVLVGYNTVFAALLILIMGFLCSGNY
                     ADSKDSYQYYAVSLLIYGMLNGAMTASNCFMERDIKRPNLRIIFSPLGSFPIYFSKIL
                     SSFVFDYSLHLLLMIILCPLLHVTLGSNPVFFILLMAPIEFAAAALGIFFCCIFHCEE
                     TTSTLLSTVISLLCVLGGTFFSFDGMGRALAFASRLSPVKWLNDAFFALSCDNSLGYF
                     WPVFIGATVLSVLLVLGCRLFFRTEDYL"
     gene            3659..>4578
                     /locus_tag="VHR42_03195"
     CDS             3659..>4578
                     /locus_tag="VHR42_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026218.1"
                     /translation="MRTFLTVLKNNYLRTVPRAIPVLVMTIITLASIMLAVYITGIQQ
                     VKGHIVFVTQSPTAVMSKSSKLLEITVSAHKPPHSDLVKQKYDAYITVDDAGNYQIET
                     LHSNDFKNMLLLLLKHPDAKVGDSKTDRGVGVNIIGFMMMFLLMLAFSNLFAFADDKE
                     QGQLHRIATAPASFGFYLAAHCIYCLSFLLPEFLLLAVLKLIGWNIGFTLLQYAGLMA
                     VLGFLGISFALLLNTLIQKPDNANMLGNSITVLTSVLAGSFYSFSKNNALLDNIIKLL
                     PQKEIMDFAQHMQGGSGWQHSGSIIYTIAFS"
BASE COUNT         1152 a         1056 c         1046 g         1324 t
ORIGIN      
        1 atccatgaac ggggtggtaa gcttagcgca ggttttgctg ttaggatgcc gggtaactat
       61 attgcctcat atggtgcctt tcctcaaagg ttgcagaaac tccttttcaa acggtcaaaa
      121 agaaaggttc ttgcaattac cactgaaata gtaatgaaaa aggttaaacg aattccgcga
      181 ggaagcgtgc tcatgcgggc atcccaaaca gcaagtcata agattctgga tagcttcgga
      241 gaaaaagcaa gaaattttcg ggtgaacggg aagtgcattg gatgtgcgat atgctgccgt
      301 atctgtccgg atggaaatat aaagattaac gagggaaaac cgatctgggg cagcatatgt
      361 gaacaatgta tggcttgcat tcaatggtgc ccatacaagt caattgagta ttccgataaa
      421 acagagaaaa gatcgcgata ccgacatccc gaagtaaatg tgacggatat tgtttcaaag
      481 cgtagttaat aaagtgcagt tcaaaggtct catatgactg tactttgcat ttgagaagtt
      541 tacttcgcag tttgtttacc aaataccaca tgctgtaaac gcgtgtgctg aaaaagtgtg
      601 tcgtcatcat ttccatttac ttttcctcga aatctacgcc cttcaggact tccgtggtct
      661 tgaaggattt ttttaaatcc tttacttgga ttgcgatgtc tttcatttag aatcgttctc
      721 ccttccgaat ttgttcatca tgtcgcggtt taattttttg cgatagttat ctgtccactt
      781 tttcgtgtcg tgcaaaagct cgtcgcagaa gccgacaaca tcttcgcccg tcacgtcgag
      841 aacgtgcctg ccgttcgccg cgccggattc aaacaactct atcaattcat actgagtttt
      901 cagcatgtct gagccgtctc ctcccgcgaa attccacatg tattcctgga ttttgttaaa
      961 tacgaaccga tagtcttcag gcaacgcttt tacccttgcc atttgctccc gatactcttt
     1021 tttgtctccg atcattttct tgataagttc cagcatcgtt attgcccctc cttattaaat
     1081 ttttctaaaa tttctttgtt tagtttttcc cgatgctttt cagtattggg tacctccgcg
     1141 cgcataaact catcactaaa cttgccgacg tcactgccta tgacatcaag aacctgtctg
     1201 ccttctgccg cgcttgcctc aaatagatcc actaagtccg taaacaacgt caagtccgta
     1261 aatatcgtca agtcaccgtc aggggggcca acggtaaaca tataatgttg aattttgcgg
     1321 aatgcaaacc gaaaatcttt cggcagggca tcaactctct tcatcatttg tttataggcc
     1381 cgtttttctt ccaaatcgcc tattatcaat tttataatcg aatcgagcat cgttacggct
     1441 cctcctttag ttggttgatt ttcgacgcga caaattccca cttttcccag aacctccgca
     1501 actcctcacg tcctgcgtta ttgagcgcaa aaaactttcg cggcggcccc atgtcggagg
     1561 gttttttggt gatctccacc agcttgtttt tttcaagccg gatcaggatg gtgtacaccg
     1621 tcccctccac aacatctgtg aagccgaggg cgttgagccg ccgcgtgatt tcgtagccgt
     1681 aggtttcttt gtggcttata atttcaagga cgcagccctc aagcacgcct ttgagcattt
     1741 ccgttaggtt ttccagcaca atcactcctt tactattctg tatgactaat aatacgtatt
     1801 acttactact ggtacatagt aacacgtaat agctagtttg tcaatagcgt ttgctctgac
     1861 cattagcagt agtttgaata tttagaaaaa tcaacccatc aaatcaaccg aacggttgat
     1921 ttttttgtta taggaataaa gtatgataat acgcgggaga tgatttaaat ggaaaatatc
     1981 atgactgtga aaaacctgac gaaaagcttt aaagacaaca aagttctcaa agggatcagc
     2041 tttgatgtcc tgcggggaga agtactctgc attctggggc cgaacggcgc gggaaaaagc
     2101 acgacgatta atatcctgac cgcggcgctc ggcagtgacg gcggtgagat aaattttaaa
     2161 ggcaaaaaaa tcagacgcca tctgcgcgct tacaagcagg tgctgggaat tgttccgcag
     2221 gatatcgctc tgtatgatga gttgagcgcc gagcgcaatc tgagattttt tgcttctctg
     2281 tacgggctgc atggcgacgc actcaccaat gcggtgaatg aggcgcttat attctccggc
     2341 cttgacgacc ggagacatga taaggtaaaa accttctccg gcggcatgaa gcggcggctc
     2401 aacatcgcct gcgccattgc ccatcacccg gagcttgtca ttatggatga accgaccgta
     2461 ggcatcgacc cgcagagtcg caatcatata ttagactcta tcaaaaagct tcgtgacagc
     2521 gggatgaccg ttatctacac cacccactac atggaagaag tcgaggcgat ctcttcgcgg
     2581 attattatca tggatcatgg cgctattatc gccgaaggca cgaaggaaac actcaaggaa
     2641 gacatagcaa acgaaaagcg ttacgctatt caggttgaag aaaacgggaa gatcccggcc
     2701 gaagaatttt attcgattga gggcgtgaaa tcggcaaaaa ttgacggtga ttccattgaa
     2761 atcacttccc tcaaaggaat tgaaaatctg gatcatatta tcgcgctgct gtgtgaccgc
     2821 ggcgagaaaa tacgaaatat tacaagcgag actgcaagtc tggaaatggt ctttttaaag
     2881 ctgaccggcc gcaagctgcg cgattgagag ggggagtaaa aatgcgccgc tttttttcta
     2941 tttttaaaat ggatatgatc aatcttttta aaaacccggt actggtcggg tataacacag
     3001 tatttgccgc gcttttaata ttgattatgg gctttttgtg cagcggcaat tacgcagaca
     3061 gtaaagactc ctaccaatat tacgcggttt cacttttgat ttacggaatg cttaacggag
     3121 ccatgactgc gtccaactgc tttatggagc gcgacatcaa gcgcccaaac ctgcgtatca
     3181 tcttttcgcc gttgggaagc ttcccgatct atttttcaaa aattctctcg tcctttgtgt
     3241 ttgactacag cttgcatctg ctgctgatga ttattctttg tccgttgctg cacgtcactt
     3301 taggaagcaa ccctgtcttt ttcattctgc tgatggcacc catcgagttt gcagcggcgg
     3361 cgctgggaat cttcttttgc tgtatctttc attgcgaaga gacaaccagt acactgctca
     3421 gcacggtgat cagcctgctg tgcgtcctgg gcggtacgtt cttctcgttt gacggcatgg
     3481 gcagagcgct ggccttcgcc tcgcgcctgt cgccggtaaa atggctgaac gatgcttttt
     3541 ttgcgctgtc ctgcgataac agcctcggct atttctggcc ggtctttatt ggcgcgactg
     3601 tcctatcggt gctgcttgta ctgggctgcc gcctgttttt cagaacggag gattatctat
     3661 gagaaccttt ttgacggtac ttaaaaacaa ttatctgcgt acggtgccgc gtgcaattcc
     3721 ggtgcttgta atgacaatta ttacgcttgc atcaattatg ctcgcagttt atattaccgg
     3781 catccaacag gtaaaaggac acatcgtttt tgttacgcaa agtccgaccg ctgtcatgtc
     3841 aaaaagctca aagcttcttg aaataactgt atcggcgcat aagcccccgc attccgatct
     3901 ggttaagcaa aaatatgacg cttatataac ggtggatgat gcgggaaatt atcagattga
     3961 gacgctgcac agcaacgatt ttaaaaatat gctgctgttg ctgctcaaac atccggatgc
     4021 aaaagtcggt gacagcaaaa cagaccgcgg cgtgggtgta aatatcattg ggtttatgat
     4081 gatgttcctg ctgatgcttg ctttttcaaa cctgtttgcc ttcgcggacg acaaggagca
     4141 gggacaattg catcggattg cgacggcgcc cgcttccttt ggattctatc ttgcggcgca
     4201 ctgtatctat tgcctgtcgt tcctgctgcc tgagtttctc ttgcttgccg tgttgaagct
     4261 gatcggctgg aatatcggtt ttaccctttt gcagtatgcg ggcctgatgg ctgtgcttgg
     4321 ctttttgggt atatcttttg cgctgttgct caataccttg attcaaaaac cggataacgc
     4381 caacatgttg ggcaattcga tcacggtgct gacatcggtg ctcgcgggca gtttttactc
     4441 gttcagtaaa aacaatgcgt tgcttgacaa cattattaag cttcttccgc agaaagagat
     4501 catggatttt gcacagcata tgcaaggcgg cagcggatgg cagcatagcg gttctattat
     4561 ctacaccatc gctttttc
//
LOCUS       DATALY010000110        12554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_458122,
            whole genome shotgun sequence.
ACCESSION   DATALY010000110 DATALY010000000
VERSION     DATALY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458122"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /gene="thrS"
                     /locus_tag="VHR42_03200"
     CDS             <1..1477
                     /gene="thrS"
                     /locus_tag="VHR42_03200"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX3026219.1"
                     /translation="LMEERSEPYKIELIGEHASKGEKISFYRQGEFVELCAGPHIPDT
                     SRVKAFKLTQCTGAYWRGDAKNKMLKRVYGVAFPKASELEAYLTAVEEAKKRDHRKIG
                     KALDLFDIYPEGPGFPFFLPKGMIIRNTLENFWREQHQKRGYQEIRTPMILNEELWHN
                     SGHWDHYKDNMYFTKIDDEDYAIKPMNCPGGILVYKRKPYSYRDLPLRLAELGLVHRH
                     ELSGTLHGLMRVRCFTQDDAHIFMTPEMIESEIIGVIDLIDYFYGVFGFKYHVELSTR
                     PENSMGSDEQWEVATAALKNALVDKGMDFKVNEGDGAFYGPKIDFHLEDCIGRTWQCG
                     TIQLDFQMPERFDLHYVGADGEKHRPVMIHRVVFGSIERFIGILIEHYAGALPIWLSP
                     VQVKVLPLSERHAEYAQKVYDRLVEAGIRTEIDDRNEKIGYRIREAQLEKMPYMLVAG
                     DKEAESGTVSVRSRKEGDQGAVKLEQFIQMIQKEIAEKSNQ"
     gene            1946..2167
                     /locus_tag="VHR42_03205"
     CDS             1946..2167
                     /locus_tag="VHR42_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HEX3026220.1"
                     /translation="MRNLFKPGNRLIVKQVNYQHRAGKMLIDMGITPETMLLVDRVAP
                     LGEPFIIKVRDYSLALRKSDLMALTVELR"
     gene            2200..4026
                     /gene="feoB"
                     /locus_tag="VHR42_03210"
     CDS             2200..4026
                     /gene="feoB"
                     /locus_tag="VHR42_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX3026221.1"
                     /translation="MQIVLAGNPNSGKTSLFNYLTGSKQHVGNWPGVTVEKKEGMISA
                     EGKNHVLVDLPGIYTLDPDTIEQKVARDYILKEKPDLIINILDATNLRRNLYFTLQLR
                     EFGVPMVMVLNMMDEVNKNGTIIHLERLRKIFGVPVIGISAAKGTGIQDLRGLIAEVD
                     MHKIDIQPFCSGCESCSKCQSGEYRYRFIDNIIEQCVETSETKKRSGLTERIDRIVLN
                     KYLAFPIFFVIMFLMFSLTFGGLVTKLSDVIEFLLSDVVKKLLLDLLTIISAPAWLIS
                     LVCNGIISGIGTVLIFLPQIAALFILMSLLEDSGYMARAAVMMDKVFSFFGLSGSSFI
                     PLIMGFGCAVPAVMACRILPSEKDRKLTIMLTSFISCSARLPLYALLAGVFFSRYRGL
                     VVFSVYMLGILVAILSAILLNKTIFKSNSASFVMELPPYRLPKLKNLVLHTWERVKGF
                     LIKAGTVLLAASVVVWFFQSFTVSFQMTSNPENSLWAAIGRGISPLFAPLGFGNWIPA
                     TALITGIPAKEMVVTTISVLTGANGNTSALHQALGGVFSPLSAYSFMCFTLLYTPCIS
                     TIVMMKREFNSLKWTVGTIAFDFSVAWVVTFLIFQVGRMFGL"
     gene            4078..4509
                     /locus_tag="VHR42_03215"
     CDS             4078..4509
                     /locus_tag="VHR42_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX3026222.1"
                     /translation="MEEAKKETRNTKQKKFITDCLKNYPNSHLTAEEIYHLIKENDQH
                     ISIATVYRNLKLLEEQGVIRKNIVTEEASSFYELTDKSEPHSHHHLVCRQCGAIIDFE
                     ADLLDSLEKMVEATTGFNIEDHRVVFYGKCKKCSAQNKQGQ"
     gene            complement(4641..5750)
                     /locus_tag="VHR42_03220"
     CDS             complement(4641..5750)
                     /locus_tag="VHR42_03220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3026223.1"
                     /translation="MKTWNTSPISPFGQFTDPALEREYQNFRFRRLKNKILPVAVILS
                     VLYFLFLLPDCFFNANRDGFYASLAVRIFSVALFFLIYFALDRVKVVKSYHILILIYE
                     LYFPFSYLLLIHLFKNHNLMINCMDVILIDLLIFAIPSYWLLTISTAFFNFLLFFIYA
                     HFGYSGLPAVDYAAVVIYLLVIYFINCIAVYRTHVYKRKQFLDKKKLRQLAMTDSLTG
                     ILNRAGFSRSLENLLALKARNGKPYCLAILDIDDFKMINDTYGHITGDHVLRRLCDVI
                     NNAKRPSDLLARWGGEEFIMIMPSADLSAAEYLVENIRRAIEQSKFPMGTGITCSFGV
                     TAIKNSDTPESLVQRADLLLYHSKRAGKNMVTARK"
     gene            complement(5835..6530)
                     /locus_tag="VHR42_03225"
     CDS             complement(5835..6530)
                     /locus_tag="VHR42_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3026224.1"
                     /translation="MEKKYYETFKSASLFSGIGGQALESLLNCLNPMIKSFHNQETIA
                     VAGSRFSGIGVVLEGKASIFKENFAGDRVLIDQVQPGEMFGEMAAFSPAGVWPATVSA
                     TDRCVAAFIPPSVFTGTCPNTCEHHQQMIRNLLGILTRKALNLNGRLEYLSICGVRQK
                     LCTYLADLSRKQKSATIFLPLNRNETADFLNIPRPSLSRELCKMRDEGLIRFRKSSVE
                     ILDSDKIHQSIGL"
     gene            6683..7408
                     /locus_tag="VHR42_03230"
     CDS             6683..7408
                     /locus_tag="VHR42_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3026225.1"
                     /translation="MIRKIVEIDQGKCNGCGLCVNACHEGALQMVNGKAQLVSDSYCD
                     GLGDCLPECPVDAIRIVERQAEIYQQELVDQKKKENQIQAVEKEEIVHGCPGMRAATI
                     RRHSPQTDGQAEPHQCVKSELGQWPVQIKLVSPIAPYFNNASILVAADCTAYAYAGFH
                     QDFMRGKITLIGCPKLDQTDYSEKLAEILRQNKIRDITVVRMEVPCCGGMTNAVKKAI
                     AESGNIVPWKVVVVGTDGTLIEE"
     gene            7441..9003
                     /gene="hcp"
                     /locus_tag="VHR42_03235"
                     /gene_synonym="priS"
     CDS             7441..9003
                     /gene="hcp"
                     /locus_tag="VHR42_03235"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HEX3026226.1"
                     /translation="MSMFCFQCEQTAGGKGCRVSGVCGKKPDVAGSQDELTCALIELS
                     RAAKGKAPGKKAAELIMKGLFTTITNVSFDKESIQSLTGEIRRETERLGGAEEYPQKQ
                     LWEGDTDTVSLRSTLLFGLRGMAAYAWHAYVLGKEDGEVTDWFFRGMYELGREHSGEE
                     WLNLILEFGNINLKCMALLDQANTSAYGQPTPVGVTANIEKGPFIVISGHDLKDLEML
                     LRQTEGRGVNVYTHGEMLPAHGYPNLKKYSQLKGNFGTAWQNQQKEFENLPAPVLFTT
                     NCLMPPKPSYTDRVFTTEVVQYPGLVHIEEDETGNKDFSLVIDKAIELGGYPEDRSMT
                     GINGGSQMMTGFAQNAVLSAAGEVIEAVKSGALKHIFLVGGCDGARPGRNYYTDFVKK
                     APSDTIILTLACGKYRFNDLDLGKIGNLPRIMDMGQCNDAYSAIRVALALADAFGCSV
                     NDLPLTLVLSWYEQKAVCILLTLLALGIRNIYIGPTLPAFLSSNVLGLLVEKFGLKPI
                     TTPEDDLKAILG"
     gene            complement(9074..9601)
                     /locus_tag="VHR42_03240"
     CDS             complement(9074..9601)
                     /locus_tag="VHR42_03240"
                     /inference="COORDINATES: protein motif:HMM:NF040142.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026227.1"
                     /translation="MSLLNILRFIKYKIIKTNLFVFFLISIFMTLLAERLPMWLYYYK
                     NWMYKERKWEDGGRIYERLFFVRKWKCRLPEISEFLKWRFSKKHLTEKNNPAYLTKFL
                     VESCKSEFTHWMIILSTILFIFWSDLYTMSRTMMLALILNIPYIIIQRYNRPRIIKLI
                     QKNDVEHYELSPVKA"
     gene            9847..10791
                     /locus_tag="VHR42_03245"
     CDS             9847..10791
                     /locus_tag="VHR42_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026228.1"
                     /translation="MQNKHFIVILIMGVFLLSSCAEKNGIHKIVVYSGDSESVSQSGG
                     AESGSQVSRSASTGGGEDCTQADFGSKSVRSSSGGQKAGIRSSVGGQSTEGGSGASSV
                     SADPIELYNRAALLLNQAQGLRFDFENCYIGKSQRTDRGSVRLEKGGSLKLSEDLTVS
                     MAGAPTDKRVLYYYNGRTMYEQKFIGDTRSTVRETPGDTPDRCNVLLYPRLQRSSVKN
                     ASCAKSPNGWTVRLELGPSDVYHGVYGILDPDYQQVSGDQITSVSQEVRLTSAGWISS
                     SVQTIQLRYKGAGLTFRMSKTFDQPGSTVTVTPPGFVY"
     gene            complement(10875..11867)
                     /gene="ilvC"
                     /locus_tag="VHR42_03250"
     CDS             complement(10875..11867)
                     /gene="ilvC"
                     /locus_tag="VHR42_03250"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX3026229.1"
                     /translation="MAKMFYESDCNFSLLKGKKIAIIGYGSQGHAHALNLHESGADVI
                     VGLYHGSKSWKKAEDAGLKVMTASDASKAADIIMILVNDEKQAGLYTESIAPNMTTGK
                     ALVFAHGFNIHYGQIKPPADVDVFMVAPKGPGHTVRSEYERGAGVPCLIAIEQNYSGK
                     AKDLALAYAAGIGGARAGILETTFRVETETDLFGEQAVLCGGVTELMRAGFETLVEAG
                     YAPENAYFECIHEMKLIVDLIYNGGFEKMRYSISDTAEYGDYVTGKRIITDETRKEMK
                     KVLSEIQDGTFARNWILENRVGRPHFNAMKRQNAETELVKVGQDLRGMMKFLSK"
     gene            complement(11942..12460)
                     /gene="ilvN"
                     /locus_tag="VHR42_03255"
     CDS             complement(11942..12460)
                     /gene="ilvN"
                     /locus_tag="VHR42_03255"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX3026230.1"
                     /translation="MKHTLSVLVENRPGVLSKVAGLFSRRSFNIDSLAVGVTEDTTIS
                     RITIVVNGDDYTIEQVEKQLNKLIDVIKVKTLTPGEFISRELTLFKVHANASNRSEIV
                     QIADLFGAKIVDVARDTLTLEFSDTNERTEDLENMLKPYGIREVVRTGTIALDRGGKL
                     IGADGREKKAEE"
BASE COUNT         3317 a         2822 c         3217 g         3198 t
ORIGIN      
        1 tctgatggag gagaggagcg aaccctataa aatagagctg atcggggagc atgcctccaa
       61 aggtgaaaaa atttcttttt accggcaggg ggaatttgtc gagctttgcg cgggtcccca
      121 tattcctgac acctccagag tcaaggcgtt taaactcact cagtgcacag gcgcttattg
      181 gaggggcgac gcgaagaaca aaatgctgaa acgtgtttac ggcgtcgctt tcccgaaggc
      241 ttccgagctg gaggcttatc tgaccgccgt tgaggaagcg aagaaacgcg atcaccgcaa
      301 gatcgggaaa gctctcgatt tgtttgacat ttatcccgaa ggccccggat tcccgttttt
      361 cctgcccaag ggaatgatta tccgcaacac gctcgaaaat ttctggaggg aacagcatca
      421 gaaacgcggc tatcaggaaa tccgcacccc gatgattctc aacgaggaac tctggcataa
      481 ttccggccac tgggaccatt acaaagacaa tatgtatttc acgaaaatag atgacgaaga
      541 ttacgcgatc aaaccgatga actgtccggg aggcattctg gtttataaac gcaaacccta
      601 ctcctatcgc gatcttccgc tgcgactggc cgagctgggc cttgtccacc gccatgagct
      661 ttccggaacg cttcacggcc tgatgcgggt acgttgtttt acccaggacg acgcccatat
      721 ttttatgact cctgaaatga tcgagagtga aatcatcggc gtcattgatc tgatcgatta
      781 tttttacggg gtattcggct ttaaatatca tgtggagctt tccacaaggc cggaaaattc
      841 catgggcagc gatgagcagt gggaggtcgc cacggcggcg ttgaaaaatg cgcttgtcga
      901 taaaggcatg gacttcaagg ttaacgaagg ggacggtgca ttttacggtc cgaagatcga
      961 cttccatctg gaggactgca tcgggcgcac ctggcagtgc ggaaccatcc agcttgattt
     1021 tcagatgccg gaacggtttg acctgcatta tgtcggggca gacggcgaaa aacacaggcc
     1081 ggttatgatc catcgtgtgg tatttggaag cattgaacgc tttatcggca tattgattga
     1141 gcattatgcc ggggctcttc cgatctggct gtctccggtt caggtcaagg tgcttccgct
     1201 ttccgagcgt cacgccgaat acgctcaaaa ggtctatgac cgtctggtgg aagccggtat
     1261 tcgcactgaa attgacgacc gcaatgaaaa aatcggctac cgaatccggg aagcgcagct
     1321 ggagaagatg ccctatatgc tggtagcggg agataaagag gcggaaagcg gaacggtttc
     1381 ggttcgttcc agaaaagaag gcgatcaggg agcggtaaaa ctggaacagt tcatccagat
     1441 gatacagaag gagatcgcag agaaatccaa tcaataacca caaattatga gtagaggccg
     1501 gcgcccatcc ttcgggaatg ggcgccggcc tttttaatga gaaggggtcg ctgatcgtta
     1561 tacaatctgt tttctttctt aaaaacggcc ttccaaggct gttttttgac cattattgat
     1621 attatgtaaa ctttagggac aagctgattt ttgcacaatt gccggtttta cgtactggga
     1681 aaaacaagta aaagttagag ccgagtggct gattgggcag aatttaccct gttgaaaact
     1741 ctgtggagaa tgtggaatac tcattgtata gtaaatttcc gtcgggaatt atgtaaaata
     1801 atcctgtgat tgatcgaaat ttgaagcgta agaaactgca caaaaattaa tatttggaaa
     1861 ccggattttt tttcgtttgt ggtattgaaa tttctgcctg catatattat aatgataatc
     1921 gttatcaata acagggggcg atggtatgag aaatctgttc aaacccggca acaggctgat
     1981 tgtcaaacag gtcaactatc aacacagggc gggaaaaatg ctgatcgaca tggggattac
     2041 gcccgaaacc atgcttttgg ttgacagagt cgctccgctg ggcgagccct tcattattaa
     2101 agtccgggat tacagcctgg cattgagaaa atccgatcta atggcattga cggttgaatt
     2161 aagataatcg acgtgtcaac ggaaaaaagg aggccgaaaa tgcaaattgt actggcaggc
     2221 aacccaaaca gcggaaaaac atcgcttttc aactatttaa caggaagcaa acagcatgta
     2281 ggcaactggc cgggtgtaac ggtcgaaaaa aaagaaggga tgatatcggc tgaaggtaaa
     2341 aatcacgttc ttgtggatct tcccggtatt tatacccttg atcctgacac aattgagcaa
     2401 aaagtcgcgc gggattacat tttaaaagaa aagcccgacc tgattattaa tattctcgac
     2461 gccaccaatc tcagacgaaa tctttatttt acgcttcaac ttcgtgaatt tggcgtgcca
     2521 atggtcatgg tgttgaatat gatggatgaa gtgaacaaaa acggaacgat cattcatttg
     2581 gagcggctca ggaaaatctt tggtgtaccg gtgatcggca tctcggccgc caaagggacg
     2641 ggaatacagg atcttagggg tctgattgcc gaggtcgaca tgcataaaat tgacattcaa
     2701 ccgttctgca gcggatgcga aagttgctca aaatgtcagt caggcgaata tcgctaccga
     2761 tttattgaca acatcataga acaatgtgtg gaaacttcgg aaaccaaaaa gaggtcggga
     2821 ctgacggaac ggatcgacag aatcgtcctg aacaaatatt tggcatttcc gatttttttt
     2881 gttataatgt tcttaatgtt ttcgcttact ttcggcgggc ttgtcaccaa gctttccgat
     2941 gtaattgagt ttttgttaag cgatgtggtt aaaaaactgc tgctcgatct gcttaccata
     3001 atcagcgcgc cggcatggct gatttctctg gtctgtaacg ggatcatctc cggtatcggc
     3061 accgtattga tctttcttcc gcagatcgcc gcgctgttta ttttgatgtc gctgctggaa
     3121 gacagcggct atatggcccg ggccgcggta atgatggata aggttttttc atttttcggc
     3181 ctgagcggca gctcgtttat ccccctgatt atgggattcg gctgcgcggt tccggcggtt
     3241 atggcctgcc ggattctgcc gtcggaaaaa gaccggaaac tgaccatcat gctgacctcg
     3301 tttatatcct gcagcgcgcg tcttcctctt tacgcgcttc tggccggcgt gtttttcagc
     3361 cgttaccggg ggctggtggt tttctccgtc tacatgcttg gaatactggt ggcgattctc
     3421 agcgccattt tactgaataa aacaattttc aaatcaaaca gcgcatcttt tgtcatggag
     3481 cttcctcctt atcggctgcc gaaactcaaa aaccttgttt tacatacctg ggaacgggtt
     3541 aagggctttc tgatcaaggc ggggacggtt ttgctggctg cctcagtggt ggtatggttt
     3601 ttccagtcct tcacggtatc gttccagatg acgtcaaacc cggaaaacag cctttgggcg
     3661 gcaattggaa gaggtatttc tcccctgttt gcgccgcttg gatttggaaa ctggataccg
     3721 gcgacagccc tgatcacggg aattcccgcc aaggaaatgg tcgtaacaac catttcggtg
     3781 ctaacagggg ctaacgggaa tacgtcggcg ctccatcagg cgctcggcgg agtgttctct
     3841 ccgctgtcag cttattcgtt tatgtgcttt acacttcttt atacaccctg catttcgact
     3901 atcgttatga tgaaacggga gtttaactcg cttaaatgga ctgtggggac gattgctttt
     3961 gacttttccg ttgcatgggt tgtgacattt ctgatctttc aggtaggcag aatgtttggg
     4021 ctttagtttt tcgggagttc atgccggtga gcgccggcct taaaggagga tggtattttg
     4081 gaagaagcaa aaaaggaaac cagaaatacc aaacaaaaaa aatttatcac cgattgcctt
     4141 aaaaattatc ccaacagtca tttgaccgcg gaagaaattt accacctgat aaaggaaaac
     4201 gatcagcata tcagtattgc aaccgtttac cggaatttga agctgctcga agagcagggg
     4261 gttatccgaa agaatattgt tacggaagaa gcgtcctcct tctatgagct taccgacaaa
     4321 agtgaaccgc attcccatca tcacctggtc tgccggcaat gcggcgcgat aatagatttc
     4381 gaagcagatc tgctggattc gctggagaaa atggttgaag caacgacggg attcaatatt
     4441 gaggaccatc gggttgtttt ttacggaaaa tgtaaaaagt gcagcgcgca gaataaacag
     4501 ggccaataaa catgggcaat atgaactgaa tcacaaaagt aaagccgtcc ttattacgag
     4561 gacggcttta tcattaaaaa ataaaaatca agttttgtct gacttttggg ggtgtacttc
     4621 agtacagccc gttttattat ctattttcgt gcggtcacca tgttttttcc ggcgcgtttg
     4681 gaatgataaa gcagcaaatc agcacgctgt accagtgact cgggggtgtc gctgttttta
     4741 atggcggtga cgccgaaact gcaggtgata cccgttccca tcggaaattt cgattgttca
     4801 atggctcttc ggatgttctc gaccagatat tcggcggccg acagatcggc agaaggcata
     4861 atcatgataa actcttctcc tccccatctg gccagcaggt cggatggacg tttcgcattg
     4921 ttgatgacgt cgcaaagccg tctcagaaca tggtcgccgg tgatgtgtcc ataggtgtcg
     4981 ttaatcattt taaaatcgtc aatatccaga attgccagac aataaggctt cccgttccgc
     5041 gctttgagtg ccagaagatt ttcaagtgag cggctgaatc cggcccggtt tagaataccg
     5101 gtcaggctat cggtcatcgc cagctggcgc agttttttct tatcaagaaa ctgcttccgc
     5161 ttatagacgt gcgtccgata gacggctatg cagttgatga aataaataac aaggagatag
     5221 atgaccacag cggcatagtc gaccgcaggc aggccgctgt atccaaaatg tgcatagata
     5281 aagaataaaa gaaaattaaa gaaagcggtg gatattgtca gcagccaata ggaaggaatc
     5341 gcaaaaatca aaagatcaat aaggataacg tccatacagt tgatcatcag attgtggttt
     5401 ttaaatagat ggatcagaag aaggtaggaa aacggaaaat acagttcata aatcagaata
     5461 aggatatggt agcttttgac cactttgacc ctgtcaagtg caaaatagat caaaaagaag
     5521 agggctacgg aaaaaatgcg aacggccaga ctcgcgtaaa atccatcccg attggcattg
     5581 aaaaaacaat caggcagaag aaatagaaag tagagaacag ataaaattac tgcaactggc
     5641 agaattttat tttttaatct tctgaatctg aagttttgat attcacgttc cagagcggga
     5701 tccgtgaact ggccgaatgg ggaaatcggc gaagtattcc aagttttcat gatcgggctc
     5761 ctgcatcatc aaattacaaa atcaattata catgaatcga cacagaaata gcaatcattg
     5821 gattcaaaaa tcacttaaag ccctattgat tgatgaattt tgtcggagtc gagaatctca
     5881 acggaggatt ttcggaaccg gatcaggccc tcgtccctca tcttgcaaag ttcccgggaa
     5941 agcgacggac gggggatatt taaaaaatcg gccgtttcgt tccggttcaa aggaagaaag
     6001 atcgtggcgg atttctgttt ccggctcagg tcggccaaat aagtgcagag tttctgccgg
     6061 acaccgcaga tggagaggta ttcaagcctt ccattcagat tcagcgcctt tcgggtaaga
     6121 attcccagca ggtttcggat catttgctga tggtgttcac aggtgttggg gcaggtgccg
     6181 gtaaaaaccg atggcggaat aaacgcggcg acgcagcgat cggtggcgga aacggtcgcg
     6241 ggccagaccc cggcgggaga aaatgcggcc atttcaccaa acatttcccc gggctgcacc
     6301 tggtcgatca atacacggtc gcccgcaaag ttctctttga agatcgacgc tttcccttca
     6361 agtaccactc caatgccgct gaaacggctg ccggctacgg caatcgtctc ctggttatga
     6421 aaggacttta tcataggatt caggcaattc aggagacttt ccagggcctg tccgccgatt
     6481 cctgagaaaa gggatgccga tttgaatgtc tcgtaatatt tcttctccat gattggctcc
     6541 aatgaaagtt ttttataaaa atagatagaa tcttatgatt tagtatcata tgttaccgat
     6601 ttgctttttc tattatagta tactgagcgt gtcaaatcaa gaagaaaaaa gatacaaacc
     6661 gatttggaaa ggttggttga agatgataag aaaaattgtt gaaattgatc agggcaagtg
     6721 caacggctgc gggctttgcg tcaacgcctg ccacgaaggc gcgctgcaaa tggtgaacgg
     6781 aaaggcgcag ctggtgtccg acagctattg cgacggcttg ggagattgtc ttcccgagtg
     6841 cccggtcgac gccatccgaa tcgtcgaacg tcaggcggaa atttatcagc aggaactcgt
     6901 cgatcagaaa aagaaggaaa atcagattca ggcagttgag aaggaagaaa ttgttcacgg
     6961 ctgtcccgga atgagggcgg cgaccatacg tcggcactcc ccgcagacag acggacaggc
     7021 ggagccgcac cagtgtgtaa agtccgaatt gggacaatgg ccggtgcaga taaagcttgt
     7081 ttcgccaatc gcgccgtatt ttaacaatgc aagtattctt gtagccgccg actgtacggc
     7141 atacgcctat gccggttttc atcaggattt tatgagggga aaaattacgc tgatcggctg
     7201 cccaaaactc gatcagaccg attattcgga aaaactggcc gaaattctca gacagaataa
     7261 aatccgggat atcacggtag taagaatgga agttccctgc tgcggcggaa tgacgaacgc
     7321 ggtcaaaaag gcgatcgccg aaagcggaaa cattgtgccg tggaaagtgg tcgtcgtagg
     7381 taccgacggt actctgattg aagaataatc caacatcagt cgaaaaaagg agagattgta
     7441 atgtcaatgt tttgttttca gtgtgagcag accgcgggtg gcaaaggctg tagagtctca
     7501 ggagtctgcg gaaaaaaacc ggatgtggcg ggaagccagg atgagcttac ctgcgcgttg
     7561 attgaattat cccgcgcggc gaaaggcaaa gcccccggca aaaaagcggc ggagcttatc
     7621 atgaaagggc tttttaccac cattaccaac gtcagctttg ataaggagag catccagagc
     7681 cttaccggag aaatccgcag ggaaaccgaa agactcggcg gtgcggagga gtatcctcaa
     7741 aaacagctct gggagggtga caccgatacg gtttcgctgc gctcgacttt gcttttcggt
     7801 ttgcgcggca tggcggctta cgcctggcat gcctatgtgt tgggcaaaga ggacggcgaa
     7861 gtgacagact ggttctttcg gggtatgtac gagctgggca gggaacattc cggagaagag
     7921 tggctgaacc tgattcttga attcggaaat attaacctga aatgcatggc cctgctcgat
     7981 caggcaaata cttcggccta cggccagcca acgccggttg gggtaacggc gaatattgaa
     8041 aaggggccct ttatcgtcat atcgggccat gatctcaaag atctcgaaat gctgctcagg
     8101 caaaccgagg gcaggggagt caacgtctat acccatggag aaatgcttcc ggcgcacggc
     8161 taccccaact tgaaaaagta ttcacaactg aaaggcaatt tcggcacggc atggcaaaac
     8221 cagcaaaagg aatttgagaa tctccctgcg ccggtacttt ttaccacaaa ttgtctgatg
     8281 ccgccaaagc cttcttacac cgaccgggtt ttcaccactg aggttgttca atatcccggc
     8341 cttgtccata ttgaagaaga cgaaaccggc aataaagatt tttctctggt gattgacaag
     8401 gcgatagaac tcggaggcta tcccgaagac cgcagcatga ccggaatcaa cggcggaagc
     8461 cagatgatga ccgggtttgc ccagaacgcc gtattatcgg cggcagggga agtgattgag
     8521 gcggtcaaga gcggagcact gaaacatata ttccttgtcg gaggctgtga cggtgcgagg
     8581 cctggaagaa attattacac cgattttgtt aaaaaagctc cttccgacac catcattcta
     8641 acgttggcat gcggaaaata ccggttcaac gatcttgatc tgggcaagat aggcaatctg
     8701 ccgagaatca tggatatggg tcagtgcaat gacgcttatt ccgccatcag ggtcgcgctg
     8761 gcgctggctg acgccttcgg gtgttctgtc aacgatcttc cgcttacttt ggttctttcc
     8821 tggtatgagc agaaagcggt ctgcatcctt cttacgctgt tggcccttgg catcagaaat
     8881 atttatatcg ggccgacact cccggctttc ctttcttcca acgttcttgg acttctggtc
     8941 gaaaaattcg ggctgaagcc catcacgacc ccggaagacg acttgaaagc aatattggga
     9001 tagaaaccaa ataaaccccc aaaaccccca aaaaacctcc cgcctgatca gcgggaggtt
     9061 ttttctgctt aatttacgct ttaacggggg aaagctcata atgctccaca tcgtttttct
     9121 gaatcagttt aataattctg ggccggttgt accgctggat aatgatgtaa ggaatattca
     9181 gaatgagcgc cagcatcatg gtccgcgaca tggtatagag atcgctccag aaaataaaaa
     9241 ggatcgtgga gaggataatc atccaatggg tgaattccga tttgcaggat tcaacaagaa
     9301 atttcgtcag atacgccgga ttgttttttt cagtgagatg tttttttgaa aaacgccatt
     9361 tcaggaactc actgatctcc ggcagacggc acttccattt ccttacaaaa aataaacgtt
     9421 cataaattcg gcctccgtct tcccacttcc gttccttata catccagttt ttgtaataat
     9481 acagccacat cggcagcctt tcggccaaaa gggtcataaa aatggaaatc agaaaaaaga
     9541 cgaacagatt ggtttttata atcttatatt taatgaatct taagatattt aataagctca
     9601 tgaaatccct tcttttcgca aaggcttatt aaattatata aaataaatgg agaatcatgc
     9661 atatcgcagg aatcaaaaaa tgccgtaaca tttatatgaa attattttat actttaaatt
     9721 tgtctttgtc aaattgccct gtttcaacaa gttctcctct ttaattttcc ggttttggga
     9781 tctttttcaa agaaatatgt tgaaatttgt cggatgatgt gttttgatat taagggaagt
     9841 gaaattatgc agaacaagca ctttatcgtt attctcataa tgggcgtttt cctgctttct
     9901 tcctgtgcgg agaaaaacgg cattcacaaa attgttgttt attccgggga tagtgaatca
     9961 gtttcgcaga gcggcggagc ggaatccggg tctcaggtct cacgatccgc ttccacgggc
    10021 ggcggggaag actgtacgca agccgatttt ggaagtaaaa gcgtccggtc ttcttccggc
    10081 ggacaaaagg ccggaatacg gtcttcagtt ggcggacagt cgacagaggg cggcagcggc
    10141 gcttcctcgg tttcagcgga tccgattgag ctttataatc gggcggcgct ccttctcaat
    10201 caggcccagg gtcttcgttt tgattttgaa aactgttata ttggaaaatc tcagagaacc
    10261 gatcgtggtt ctgtccggct tgaaaaaggc ggatcattaa aactcagtga ggatctgacg
    10321 gtttcgatgg cgggagcgcc gacggataaa agagttctct attattataa cggccggacc
    10381 atgtatgagc agaagtttat cggtgatacc cgttcgacgg tcagggagac tccgggggat
    10441 acgcccgacc ggtgtaacgt tttgctgtat cccaggcttc agcggagcag cgtaaaaaac
    10501 gcctcctgtg ccaaaagccc gaacggatgg accgtgcgcc tggagctggg gccttcggac
    10561 gtatatcatg gggtttatgg tatactggat cccgattatc agcaggtttc cggagatcag
    10621 atcaccagtg tgtctcagga ggtgcggctg acctcggcgg ggtggatatc ctcttccgtg
    10681 cagacgattc agcttcggta taagggcgcg gggctgactt ttcgaatgag taagactttt
    10741 gatcagcccg gaagcacggt aacggtgacg ccgcccgggt ttgtttactg attctccaaa
    10801 ggccgaacag ctggccgagc gaaagaaagg gcgcccgcgt gttacgcggg cgccggtcgt
    10861 gtttgtatgg ttttttattt gctgaggaat ttcatcatgc cgcgaagatc ctggccgact
    10921 ttgaccagtt cggtttccgc attctgccgt ttcatggcgt tgaaatgcgg gcgtccgacg
    10981 cggttttcca gaatccagtt gcgggcgaat gtgccgtcct gaatctcgga aagaactttt
    11041 ttcatctctt tgcgggtttc atcggtaatg atgcgttttc cggtgacata gtcgccgtat
    11101 tcggccgtgt cgctgatgga gtaacgcatt ttctcaaagc cgccgttgta gatcagatca
    11161 acgatgagtt tcatttcatg aatgcactca aaataggcgt tttcaggggc gtatcctgcc
    11221 tcgacaagcg tctcgaatcc ggccctcatc agctcggtta caccgccgca gagaactgcc
    11281 tgctcaccaa acagatcggt ttcggtctcg acccggaaag ttgtttcaag aataccggcg
    11341 cgcgctccgc cgattccggc ggcataagcc agcgcaagat ctttcgcctt gcccgaataa
    11401 ttctgctcga tggcaatcag gcagggaacc ccggcgcccc tctcgtattc gctgcgaacc
    11461 gtatggccgg gacccttggg ggcgaccata aatacgtcga catccgccgg cggtttaatc
    11521 tgaccgtagt gaatgttgaa tccatgagca aaaacaagcg ctttgccggt ggtcatgttg
    11581 ggagcaatgc tctcggtata aaggccggcc tgcttttcat cgttaacgag aatcatgatg
    11641 atatccgccg ctttggaagc gtcagaagcc gtcatgacct taaggccggc gtcttcggcc
    11701 tttttccatg atttacttcc gtgataaaga ccgacaatga cgtcggctcc gctttcatga
    11761 agattcaggg catgtgcgtg cccctgactt ccatatccga taattgcgat ctttttgccc
    11821 tttagcagac taaaattgca atcgctttca taaaacatct ttgccatttt gtaaacctcc
    11881 tcgccgatct ggtcggcttc ataaatatat attgaaaagc catatggcca gacaaacggt
    11941 gctattcttc cgcttttttt tcacggccgt cggcgccgat cagctttccg cccctatcga
    12001 gagcgattgt gcctgtgcgg acaacttccc ggattccgta aggcttgagc atattttcaa
    12061 ggtcctcggt ccgttcgttg gtatcggaaa attcaagagt cagcgtgtca cgcgccacat
    12121 caacgatttt cgccccgaac aaatcggcaa tctggacaat ttcagatcgg ttggaggcgt
    12181 tggcatgaac cttgaacagc gtgagctcgc ggctgatgaa ttcgccaggg gtcagtgttt
    12241 taaccttgat gacgtcaatg agcttgttga gctgtttttc cacctgctcg atggtataat
    12301 cgtctccgtt taccacaatg gtgatgcggg agatcgtagt atcttccgta acaccgacag
    12361 ccagactgtc aatgttaaaa gacctgcggg agaaaagccc ggcgactttt gacaacacgc
    12421 cgggacggtt ttccacaaga accgataaag tgtgtttcat aataacctcc gtttggcgtt
    12481 taccatgcgg atcagaccgg gccggcacgg attttacagg gaaccttcat cggcgtcgac
    12541 gacgcattcc agaa
//
LOCUS       DATALY010000111         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_370756,
            whole genome shotgun sequence.
ACCESSION   DATALY010000111 DATALY010000000
VERSION     DATALY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370756"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..501)
                     /locus_tag="VHR42_03260"
     CDS             complement(124..501)
                     /locus_tag="VHR42_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026231.1"
                     /translation="MEADIILLSDSIFTARTGEVHANPGAIAIRGNRIIKVGDRATVN
                     SFKGTGTKIYELGDRTVCPGFIDNHQFFTGFALKRWGPDVAGVRAENALEILKKYASG
                     LSPEWAVVGHGLSVEGWSEKEKS"
     gene            complement(526..1962)
                     /locus_tag="VHR42_03265"
     CDS             complement(526..1962)
                     /locus_tag="VHR42_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX3026232.1"
                     /translation="MVWLWIYWIILSVAVYFIPANSIKGTALTLIPAVSMFIYVYVSR
                     DFLQGFCFGTILLIEMMNKGRTPVVFINSMLGQLTDSDNMFMLLTFFTCGATISALHK
                     SGSAKSFGVWMAKKIKNKKLNLIATFIMTALMSVDDYINSLTVGTAMTPINDEAGIPR
                     EMTSYVIRSSNSAPGLFYPFGDWAYFIILQLVSLKLAKDLSHAVPLYMSFVPYLFYPM
                     VVMLVSLLVIFGVIPKIGKMKDAYHRVETGGGVLSPNSKEDVDEEVEELLRQKDVNPI
                     NFAIPVLTLFAGMILTHFNGVQAGILTLVVTGLLYIPQGIFTLNEYVQVMMDGFKDML
                     DLSLLLVFGYMIGDSMSALGFSDFIVGVARHALIPQLLPVVVFLLFCCTEYLCTLNWT
                     LYIIAMPILFKVCPAIGANLPLTIAALISAGLFGSNTCIFSDGGVVVSKGSRIDLYDH
                     GFSSMPYMIIAGVASAVLYLGAGFVFHV"
     gene            2258..2968
                     /locus_tag="VHR42_03270"
     CDS             2258..2968
                     /locus_tag="VHR42_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3026233.1"
                     /translation="MNNRYPLYSQLSDLIREKIERGDYEFGQAIPSERELAAAYGVNR
                     QTVRSAIACLVKEGLVTKWKGKGSFINKPRIDTALDTIEGFGRFLEDNGVQPSSKVLF
                     TGKRKAGFLYSRIFNLAESDFLYRILRLRLADNEPVSLEDTYIPYKLIDQIETYDFQV
                     YSLYDIFSRHDINLTLDYEKLSIIRVRNPEAKLLNIAPGSSIFLVEDRSVDADGRLVE
                     FTRSYTSGERFTFSTVMI"
     gene            2983..3711
                     /locus_tag="VHR42_03275"
     CDS             2983..3711
                     /locus_tag="VHR42_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3026234.1"
                     /translation="MSKYIDLVSLDLAEKIEEYIIKNGLKPNDALPSERHLASQWNVN
                     RLTLREAIDRLVDEDRLYKVQGRGTFVAEPKFIDDAGEFISYTDGWISDGYTICSKQL
                     HLRVIEANKTIAGHLNLNLGEKVYDLKRIRIIQDIPLTIETSYIPAKYCEGLEKHDFE
                     NHSLYSTLKKEFGIELVKQSQIFSITSLNKSESGYLGVSEGSPAFYIVGTSFNRENSP
                     VEYSIALTRSDRYEICSVLTAENQ"
     gene            3974..>4341
                     /locus_tag="VHR42_03280"
     CDS             3974..>4341
                     /locus_tag="VHR42_03280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3026235.1"
                     /translation="MIRKAMEQDLGDILAIYNHAILNTTAIYDYQVHTLEDRQAWYQE
                     KARASLPLIVYTMEDRTVGFATFGPFRPYPAYHYTVEHSVYVHPDHQGKGIGKALLSK
                     IVELADCEGYATMVAGIDSE"
BASE COUNT         1201 a         1039 c         1017 g         1084 t
ORIGIN      
        1 ggcgtagcat tcatccggtg agaacccata gttattctca gcggcgcggt tcatccagca
       61 aaagtcacga tctggtgtaa aaacctcaac cggacggctg ccaaattccc ggtcaagcat
      121 atgtcagctt ttctcttttt cgctccagcc ttccaccgac aggccatggc cgacaaccgc
      181 ccactccggc gagagaccgg aagcatattt tttcagtatc tcaagagcgt tttccgcgcg
      241 cacgccggcc acatcaggcc cccacctttt gagcgcgaat ccggtgaaga actgatggtt
      301 gtcaatgaat cccgggcaaa ccgtcctgtc gcccaactca tagatttttg tcccggtacc
      361 tttgaaagaa ttgaccgtcg cccggtctcc gaccttaata attctgttcc cccgaatggc
      421 aatggctccg ggattcgcat gaacttcgcc agtacgggcc gtgaaaatgc tgtcagacag
      481 cagaatgata tccgcttcca ttttaccgcg ttctttcttc atttattata catgaaatac
      541 aaatccggct cccagataaa gcacagccga cgcaacacct gcaataatca tataaggcat
      601 gctggaaaaa ccgtgatcat acaggtcgat gcgcgaaccc ttggaaacca cgacgccgcc
      661 gtctgagaaa atacaggtat tgctgccgaa aagaccggcg gaaatcaacg cggcaatggt
      721 cagcggaaga ttggcgccga ttgcgggaca caccttgaac aaaatcggca tggcgataat
      781 ataaagggtc cagttgagcg tacaaagata ttcggtgcag cagaacagga gaaaaacaac
      841 gacaggaagg agctgcggta tcaacgcgtg tcttgcaacg ccgacaatga aatccgaaaa
      901 tccaagcgcg gacatcgagt cgccgatcat atatccgaag accagcagca acgaaagatc
      961 gagcatatcc ttaaacccat ccatcataac ctggacatac tcgttcagcg taaagattcc
     1021 ctgcggaatg tataagagcc cggtaaccac cagtgtcaga attcccgcct gaacaccgtt
     1081 gaaatgggtc agaatcatcc ctgcaaaaag agttaagacc gggatggcaa agttgattgg
     1141 attaacgtcc ttttgacgga gaagctcctc cacctcctca tccacgtctt ctttggaatt
     1201 gggcgagagc acccctcccc cagtttcaac ccgatgatag gcatctttca ttttgccgat
     1261 tttgggaatt acgccgaaaa tcaccagcaa cgacaccagc atgaccacca tggggtaaaa
     1321 caaatagggc acaaacgaca tatacagggg aaccgcgtgg gacagatctt tcgcaagttt
     1381 gagcgatacc agctggagaa tgatgaaata agcccagtcg ccaaacggat aaaagaggcc
     1441 cggcgcggaa ttggaggacc gaatgacata ggaggtcatc tctctgggga ttcccgcctc
     1501 gtcattgatc ggggtcatgg cagtgccgac tgtaagcgaa ttgatgtaat catctaccga
     1561 catcagggcg gtcatgataa atgttgcaat cagattcaat tttttatttt tgatcttttt
     1621 tgccatccag acgccaaagc ttttggccga tccgctttta tgtagcgcgc tgatggtcgc
     1681 tccgcaggta aaaaaggtca ggagcatgaa catattatca ctgtctgtta actggcccag
     1741 catactgttg ataaaaacaa ccggcgttct gcctttattc atcatctcaa tcagcaggat
     1801 ggttccaaaa caaaatccct gcagaaaatc ccggctgaca tagacataaa taaacattga
     1861 cacggccgga atcagcgtca gcgcggttcc tttaatcgaa tttgccggta taaaataaac
     1921 agcgaccgac agaataatcc agtagatcca caaccaaacc atttttggat tttttgcagg
     1981 gtttttgcct tcaccaccct tcgctctgag atccttaatt ggattgaata gattccacga
     2041 tttgtcgggc ggatcttaac tttgttcaaa acatatggta taattagaaa ttatgggata
     2101 gaaacagatg gtatgtattc catacatgtt cgctatttaa gtataccact ctatatttta
     2161 cctgtcaata tagacagttt cgtttttttg taagtcactg atatcgtgca gatttgatct
     2221 ttcatatttg tttttcgttg gaaaggaaaa gcggcagatg aacaacaggt acccacttta
     2281 cagtcagtta agcgatttga ttcgtgaaaa aattgagcgc ggcgattacg agtttggtca
     2341 ggccattcca tcggaacgcg aactcgccgc agcttacggt gtaaaccggc aaactgttcg
     2401 aagcgcgatt gcctgcttgg tcaaggaagg attagtgacc aaatggaagg gcaaggggtc
     2461 ctttatcaac aagccccgca tcgacaccgc tttggatacg atcgaaggct tcggcagatt
     2521 tcttgaagac aacggagtgc agccttccag caaggtttta tttaccggaa aacgaaaagc
     2581 aggctttctg tattcccgga tatttaatct ggcggaatcg gattttctct atcgtatcct
     2641 tcgtcttcgt cttgcagaca acgagccggt atccttggag gacacttata ttccctacaa
     2701 actgatcgat cagatcgaga cctatgattt tcaggtctat tcgctctatg atattttcag
     2761 caggcacgac attaatctga cgttggatta tgaaaagctc tccatcatcc gggtaaggaa
     2821 tccggaggcg aaattgctca atatagcgcc cggcagcagc atatttctcg tggaagacag
     2881 gagtgtggat gccgatggaa gattggtgga atttacacgc tcttatacga gcggggaacg
     2941 atttactttc tctaccgtca tgatctgaag gaggacaatg ccttgagcaa atatattgac
     3001 cttgtcagtt tggatttagc agaaaagatt gaagagtaca tcatcaaaaa cggtctcaag
     3061 ccaaatgatg cccttccttc tgagcgtcat ctggcttctc agtggaatgt caaccggctg
     3121 acccttcggg aggcgattga ccggcttgtg gatgaagatc gtctttataa agtacaaggc
     3181 agaggcactt ttgtggcgga gcctaaattc atcgacgatg cgggcgagtt tatttcctac
     3241 accgacggct ggatctcaga tgggtataca atttgcagca agcagctcca cctgcgggtg
     3301 atcgaagcga ataaaacaat tgcgggccat ctgaatctta atcttgggga aaaggtatac
     3361 gacttgaaga gaatccggat tatacaggat atccctctga caattgagac ctcctatatt
     3421 ccagcaaaat attgcgaagg cctggaaaaa cacgattttg aaaaccactc gctgtatagt
     3481 actttaaaaa aagaattcgg catcgaactt gtgaagcaaa gccagatttt cagcatcaca
     3541 tctctgaaca aaagcgaaag cggatatctc ggagtttcgg aaggttcccc cgctttttac
     3601 atcgtcggca cctcctttaa ccgggaaaac tcccctgtgg agtacagtat agcgcttacc
     3661 cgcagtgatc ggtatgaaat ctgcagtgtt cttacggcgg aaaatcaata atcagcttcc
     3721 gatgagacat cttctgcatt ccagtccggg cggccggagc ccccgcttta gggaagcaaa
     3781 tactgggtat tttgtaattc cgctaaaaat gtatgaggct ggattttctt tttttgcaaa
     3841 ccttatagcg gtacagcagc ctccgcacta tgcggaggct gctgctgatt cattcatttt
     3901 tatctttgat tatcgggcaa cggcccgcgg ttcgcctgac aggcccaaat catctttaaa
     3961 ggagaattta cccatgatac gaaaagctat ggagcaggat ctcggggata tccttgcaat
     4021 ctataatcac gcaattttaa atacaaccgc gatttatgat tatcaagttc atactctgga
     4081 agaccggcag gcctggtatc aggagaaagc gcgcgcaagt cttccgctga ttgtctatac
     4141 gatggaagac cgaaccgtgg gtttcgctac gtttgggcct tttcggccgt atccggctta
     4201 ccattatacg gttgagcact cggtctatgt gcatccggat catcagggta aaggcattgg
     4261 caaagccctg ttgagcaaaa tcgtcgaact cgcggactgc gagggctacg ccacgatggt
     4321 agccggaatc gattctgaaa a
//
LOCUS       DATALY010000112         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_458442,
            whole genome shotgun sequence.
ACCESSION   DATALY010000112 DATALY010000000
VERSION     DATALY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458442"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VHR42_03285"
     CDS             <1..514
                     /locus_tag="VHR42_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026236.1"
                     /translation="NQVALVLQKNVLFSGTIKENLRWGNENATEEEMIHACEFAQADG
                     FIREFPDQYDTYIEEGGTNVSGGQKQRLCIARALLKKPKILILDDSTSAVDTKTDGLI
                     RQAFQKDLPNTTKIIIAQRVASVQDADKIIVLDDGRINAVGTHDELLKNCAIYREVYE
                     SQNKGSVIHE"
     gene            507..2438
                     /locus_tag="VHR42_03290"
     CDS             507..2438
                     /locus_tag="VHR42_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026237.1"
                     /translation="MSKVPPKENAKLHGRIPVQRFKPGTIRRLLSYMAEYRIQLIFVV
                     LCILLSAGASAASSLFLQTLIDKYIVPMLGQAHPVFTGLLKAILFMGFIYIIGVLSTL
                     FYNRIMVVIEQGTLKKIRDKMFTHMQSLSIQYFDTHTHGDIMSRYTNDTDTLRQAISQ
                     SMPQMFSSLVTILASFFTMLYLSVGLTAFVALFALVLLKVIKTLVGRSGTFFIKQQQS
                     LGDVNGYIEEMINGQKVIKVFCHEEKVEEEFYKKNNDLCYCATEANKYGNVTMPVVGN
                     MGYMLYVLLAVIGGAAGIAGIQNLSLTGLHALTIGTIVSFLTLSRSFINPIGQVSMQF
                     NMVVMALAGASRIFELMDEKSEQDGGYVTLVNAKMENGEVKECKERTEKWAWKHPHSD
                     GTVTYTPLRGDIRFSDVDFGYNENKTVLHNITLYAEPGQKVAFVGATGAGKTTITNLI
                     NRFYDIADGKIRYDGININKIKKDDLRRSLGIVLQDVNLFTGTVMDNIRYGRLDATDE
                     ECIAAAKLANADGFIRMLPNGYNTVLEGDGSGLSQGQRQLISIARSAVADPPVMILDE
                     ATSSIDTRTEAIVQKGMDALMEGRTVFVIAHRLSTVQNSDVIMVLEHGVIIERGSHEQ
                     LIEQKGQYYQLYTGAFELE"
     gene            complement(2553..2810)
                     /locus_tag="VHR42_03295"
     CDS             complement(2553..2810)
                     /locus_tag="VHR42_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026238.1"
                     /translation="MNSFFSAGFPLNLGYAVLRRAEGENDGFVSIESSKWGDYLGCFS
                     TKRRRGVSHGDMIDLMRENIREFDVCECYVDVVKGLKAKGL"
BASE COUNT          794 a          710 c          703 g          735 t
ORIGIN      
        1 taatcaggtg gcgctggttt tgcagaaaaa cgtgctgttc tccgggacaa tcaaagaaaa
       61 cctccgctgg ggcaatgaaa acgcgactga ggaagaaatg atccatgcct gcgagtttgc
      121 ccaggccgac ggctttatcc gggaatttcc ggaccagtac gacacctata ttgaggaggg
      181 cggaacaaac gtttccggcg gccagaagca gcggctctgc attgcccggg cgctgttgaa
      241 aaaaccgaaa attttgatcc ttgacgactc cacgagcgcg gttgatacga agaccgacgg
      301 attgatccgt caggcgtttc aaaaggatct cccgaatacg acaaagatta tcatcgctca
      361 gcgcgttgcc tcggttcagg atgccgacaa aatcatcgtg ctcgacgatg gcagaatcaa
      421 tgcggtcggc actcatgatg aacttcttaa aaactgcgcg atctacaggg aagtttatga
      481 gtctcagaat aaaggaagtg ttattcatga gtaaggttcc accgaaagaa aatgcgaagt
      541 tacatggcag aatcccggta caacgattca aacccggcac gatcagaagg ctgctttcct
      601 atatggcgga gtatcgaatc cagttgattt tcgttgtcct ctgcatcctg ttgagcgcgg
      661 gcgcaagcgc ggcttcctct ttgttccttc agacactgat tgacaagtac attgtcccaa
      721 tgctgggaca ggcgcatccg gtatttacag gcctgctcaa agcgatcctt ttcatgggtt
      781 tcatctatat tatcggagtg ctttcaacat tgttctacaa cagaattatg gttgttattg
      841 agcagggcac gctgaaaaaa attcgtgaca aaatgtttac gcatatgcag tccctgtcga
      901 ttcagtattt tgacacacat acccacggcg acatcatgag ccgctacacg aacgataccg
      961 acacactgcg ccaggcaatt tctcaaagca tgccgcagat gttttcctcg cttgtcacga
     1021 tcctggcctc attttttaca atgctttatc tcagcgtcgg ccttactgct tttgtggctc
     1081 tgtttgccct cgttttgcta aaggtgatca agaccctggt gggcagaagc ggaaccttct
     1141 tcataaagca gcagcaatct ttgggcgacg tgaacggtta tatcgaagaa atgatcaacg
     1201 gtcagaaagt cataaaggta ttctgtcacg aggaaaaggt tgaggaagag ttttacaaaa
     1261 agaataacga tctctgctac tgcgctaccg aagcaaacaa gtacggaaat gttacgatgc
     1321 cggttgtcgg gaatatgggg tatatgctgt atgtgcttct cgcagtaatt gggggcgctg
     1381 ccggtatcgc aggtattcaa aaccttagtc tcaccggttt acatgccctg acaatcggta
     1441 cgatcgtttc cttccttacc ctgtcccgca gtttcatcaa cccgatcgga caggtttcca
     1501 tgcagttcaa catggtggtc atggcacttg ccggcgcatc cagaattttt gagttgatgg
     1561 acgaaaaaag cgaacaggac ggcggatacg ttacgctcgt caatgcgaaa atggaaaacg
     1621 gcgaggtaaa agaatgtaaa gaacggacgg aaaaatgggc atggaagcac ccgcacagtg
     1681 acggcacggt aacctatact ccgctgcggg gtgacattcg tttctccgat gtggactttg
     1741 gctataacga gaataagact gttctgcaca atatcacgct ttacgctgaa cccggtcaga
     1801 aagtcgcgtt tgttggtgcg accggcgcgg gaaaaacgac aattacaaat ctgatcaacc
     1861 gtttctatga tattgccgac gggaaaattc gttacgatgg catcaacatc aataaaatca
     1921 aaaaagacga ccttcgtcgg tcgcttggca ttgttctgca ggatgttaat ttgtttacag
     1981 gaacggtcat ggacaatatc cgttacggca ggctggacgc gacggatgaa gaatgtattg
     2041 ccgcagcaaa acttgcgaac gcagacggat ttatccgcat gcttccgaat gggtacaata
     2101 ccgtgcttga aggagacggc agcggccttt cacaaggaca acgccaactg atctccatcg
     2161 cccggtcggc agtggcagac ccacccgtca tgatcctcga cgaagcgaca tcctccatcg
     2221 acacccggac ggaggccatt gtccagaaag gcatggatgc attgatggaa ggcagaaccg
     2281 tatttgtcat cgcccatcga ctttctactg tgcagaattc cgacgtcatt atggtgcttg
     2341 agcatggcgt tatcattgag cggggcagcc acgagcagct cattgaacaa aagggtcaat
     2401 actatcaact gtatacaggc gcgtttgagt tggaataaat cataacaaga tcacacagta
     2461 aaaccgggat atagccattc gatcatgagc tacatcccgg tttttcctgc gcatctgtgg
     2521 gtacagtacg aatctacccg tcttttcgta tctcataaac cttttgcttt caaacctttt
     2581 acgacatcca cataacattc gcaaacatca aactctcgaa tattctcccg catcagatcg
     2641 atcatatcac cgtgcgaaac gcctcttctg cgtttggtgc tgaagcagcc gaggtagtca
     2701 ccccatttgg atgattctat cgatacaaat ccgtcgtttt ccccttctgc gcggcgcaga
     2761 acggcgtagc ccaaattcag cggaaacccc gcgctgaaga aactgttcat ttgtgatgcg
     2821 ataaacccac tttttttgac ttctttcggt taccttgcca aatagtgcac ttttttaaag
     2881 catcggtctt taatatttgc cttaaaacaa aaatattgta aagtagccaa attaatccta
     2941 ta
//
LOCUS       DATALY010000113         8487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_199380,
            whole genome shotgun sequence.
ACCESSION   DATALY010000113 DATALY010000000
VERSION     DATALY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199380"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VHR42_03300"
     CDS             complement(<1..541)
                     /locus_tag="VHR42_03300"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX3026239.1"
                     /translation="MPLNQDIKFLKGVGPRRAELFAKCGVSTVGGLLRFYPRVYEDFT
                     SPVPIASAQPGQICCIRATVASPVREARIRKGMTLYKFRAADDTGVCGITYFNNPYIK
                     NSMVEGREYFFYGKIGGTLLRREMTSPEFEPADSGTGLRPVYPLTAGLTVRMVRAAVS
                     QALKLTMENLNDPLPESLRK"
     gene            complement(621..2246)
                     /locus_tag="VHR42_03305"
     CDS             complement(621..2246)
                     /locus_tag="VHR42_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX3026240.1"
                     /translation="METISGQLLKDMLISASNAIGTQKQEVNELNVFPVPDGDTGTNM
                     SMTMGSAMRELSKLSAQTVTEVADLAATSLLRGARGNSGVILSLLFRGIAKGLKDKAE
                     ASGTDLAAALQAGVDSAYKAVMKPAEGTILTVARESAQEAARASSQGGGALAVFQAAV
                     NSAKQALANTPELLPVLKKAGVVDAGGKGLVIIFDAMLYTLSGNGILQAEEPAQTAAE
                     ISGAAGEVDDEEINFTYCTEFIINKSTAFAKDPLSLRAYLESIGDCVLVADDSNFIKV
                     HVHTDNPGKVIQEALGFGYLTDMKIDNMRVQHERKAAAKSEPVPPEKPYGFVVVAAGK
                     GVADLFKDLGADTIVEGGQTMNPSTEDILAAIESTPAENVIVLPNNKNIILAAEQTVS
                     LADRKVHVLHTKTIPQGISALLSFDADTALNDNLLEMEAACDHVKTGLITFAARDSDF
                     EGHKIKKDEILALANGKLSFTDNDPVKAAVRLTKSLSDKDTSYVTLIYGEGISPEQAE
                     AARKAAAEKLGPQVEVNLINGGQPVYHFILSVE"
     gene            complement(2340..2690)
                     /locus_tag="VHR42_03310"
     CDS             complement(2340..2690)
                     /locus_tag="VHR42_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX3026241.1"
                     /translation="MIRFENPNGLIEVNHEYFSNLIGYAASSCYGVAGMAVSSTAQGL
                     ASIFGQDKIDRGVKVRSLGKSLVVDLHIVVTYGVNITAIVKSIIKKVRYTVEEATGLR
                     VDSVNVFVDGMKTD"
     gene            complement(2825..4315)
                     /locus_tag="VHR42_03315"
     CDS             complement(2825..4315)
                     /locus_tag="VHR42_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3026242.1"
                     /translation="MKRPYQTALVVILAFLLCFAGCGKKESKNNSSSQTSKVTSKAVT
                     GSTTLSLPCVSNKSFNPYTTDSMTNLELWPLFYDCLAEPDQNFNPVLRLASAVSSQNN
                     VFTVTLRSGVVFSDGSPLTAADVKYSFELAMGNTGGYFYPRVSNIANIETSGNDIIFN
                     LKTSDPLLTNLLNLPILKQETGTSDTPTGSGIYKLASSGTDVITLTANSLWYKKTTPN
                     LKTIRLVNIPDKNALMSSLEIGNIDFLHSDYGSGMQSSVNVSSKPVSMNQLIFIGVNS
                     TRSPLNNVHLRKAVSYAVNRDSLVTEIYSGRTLSSILPLYPNLTGLKSTDKSKLKSDS
                     DSFKNELKSFGSTSSLDLALLVNQDNSVRALAADYLVSNLRKAGLNISVEKVTSQVYN
                     SRITSGNFDLYLGEVKLRSDMDLSSFLQSGGSSYGIGQKTATLTAYNNWRNGNGTLDA
                     FAAAFENEMPFIPLCYKTGAVSYHTGLSGVVSTDGNLFYNIETWHF"
     gene            complement(4494..4754)
                     /locus_tag="VHR42_03320"
     CDS             complement(4494..4754)
                     /locus_tag="VHR42_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEX3026243.1"
                     /translation="MEIIKVSSKSAPHAVAGAIANVVRDKNAAEIQSVGAGATNQAIK
                     SIAIARGYLAPSGIDIVCIPGFANVEVGGESRTAIRLIVKVI"
     gene            complement(4855..5625)
                     /locus_tag="VHR42_03325"
     CDS             complement(4855..5625)
                     /locus_tag="VHR42_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX3026244.1"
                     /translation="MNILAIGDVVGKAGCDFLREHLQALKKINQIDFVIANGENSAEG
                     NGITPASAEHLFTSGVDVITTGNHVFRRREVFNYLEENSCIIRPANYPEGAPGNGVCV
                     VDTGKISICVVNLIGTVFIDNFDNPFKAIDHILAGIDGNPIIIVDFHAEATSEKRALA
                     FYLDGRVSAIFGTHTHVQTADEEIFPGGTGYLTDVGMTGPSRSVLGVDSKNVIKRFLT
                     KMPVRFSNAEGPCLLSGVIFDIDYKTRRTNFMKRLQIR"
     gene            complement(5803..7362)
                     /gene="rny"
                     /locus_tag="VHR42_03330"
     CDS             complement(5803..7362)
                     /gene="rny"
                     /locus_tag="VHR42_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX3026245.1"
                     /translation="MGTYVVVGVIAAVAAAIVFGILAFFLGAAYRKRVAEAELGSAEE
                     ESKRIVSEALKSADSKKKEVILEAKDYIHRMRTEAEKEIKERRVDVQRQERRLQQKEE
                     TLDRKIEQLEVKDENLNKKNKESEERLHEAEEVKKRQFEMLERISGFTVEQAKDFLLT
                     NLENELTYEKSVKIKEYEQQLKEDSEKKAREIISLAVQKCAAEHVAEATVSVVPLPND
                     EMKGRIIGREGRNIRTLETLTGVDLIIDDTPEAITLSSFDPVRREVARISLERLISDG
                     RIHPARIEEMVEKARKEVDNTIRQEGERAVIEAGIHGIHHELVKLLGRLRYRTSYGQN
                     VLNHSLEVSHLSGVMAAELGVDVSTARRAGLLHDIGKAVDHEVEGSHVEIGVDIAKKY
                     KEHADIIHAIEAHHGDVEAHTVIACLVQAADAISAARPGARRENLENYIRRLEKLEDI
                     ANSFDGVEKSFAIQAGREIRIMVKPEIVDDGKITLMARDIVGKIEDELDYPGQIKVHV
                     IRESRAIEYAK"
     gene            7995..>8487
                     /locus_tag="VHR42_03335"
     CDS             7995..>8487
                     /locus_tag="VHR42_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX3026246.1"
                     /translation="MIQITLKGGVVREYEPGVTPMEIAKSLGAGLYKAACAAKIDGRL
                     ADLRTPVTADSAVEILTFAEEEGKKTFWHTASHILAQAVKRLYPNVKLTIGPAIDNGF
                     YYDFDSEIAFSAEELEKIEAEMKKIVKEGLQLERFELSADEAVRLMEERSEPYKIELI
                     GEHA"
BASE COUNT         2093 a         2042 c         2065 g         2287 t
ORIGIN      
        1 tcttccttaa ggattccgga agagggtcgt tcagattctc cattgtcagt ttaagcgcct
       61 gagaaacagc ggcccgcacc attctgactg tcagccccgc ggtcagagga taaacgggcc
      121 gcagccctgt tccgctgtcg gccggctcga attccgggga ggtcatttcc cgtcggagca
      181 gtgttccgcc aattttccca taaaaaaaat attctctgcc ttcgaccata ctatttttaa
      241 tataggggtt gttaaaatag gtgatgccac agactccggt gtcgtcggcg gcccggaatt
      301 tatacagtgt catgcctttg cggatgcgag cctcccggac aggggaagcg acggttgccc
      361 gaatgcagca gatttgcccg ggctgggcgg aagcgatcgg caccggggag gtaaaatcct
      421 catatacccg cggataaaac cggagcaggc cgccgacggt ggacacaccg catttggcaa
      481 aaagctccgc acgtcggggc ccgacgcctt tgagaaattt aatatcctga ttaagcggca
      541 taattcggct cctttccggt gctgtaaatc aaaaggggga aacttttccg tttccccctt
      601 ttttatattc aaggcagtcg ttattccact gataagataa agtgatagac cggctgtccg
      661 ccgttgatca ggttgacttc aacctgtggg ccgagttttt cggctgccgc cttgcgtgcc
      721 gcttcggcct gttcgggaga aattccttcg ccgtaaatca gtgtgacata agaagtatcc
      781 ttgtcggaaa ggcttttggt cagtctcacc gcggctttta ccgggtcgtt gtcggtaaag
      841 gaaagttttc cgttggcgag ggcaagaatc tcatcttttt tgattttatg gccttcaaaa
      901 tcggaatccc ttgcagcaaa tgtaatgagg ccggttttaa catggtcaca ggccgcttcc
      961 atttccagca aattgtcgtt cagggcggta tccgcatcaa agctcagcag tgcggaaatg
     1021 ccctgaggga tggttttggt atgtaaaaca tgcacttttc tatccgccag agagacagtc
     1081 tgctcggcgg caagaataat gtttttattg ttgggcagaa cgataacgtt ttcagcaggg
     1141 gtcgactcaa tagcggcaag aatatcttcc gtgctcggat tcatcgtctg accgccttcg
     1201 acgatggtat cggctccgag gtctttaaat aaatcagcca cgcctttgcc cgcggccacg
     1261 acaacaaacc catagggctt ttccggggga acaggttctg atttagccgc ggctttgcgt
     1321 tcgtgctgca ctctcatatt gtcgattttc atatcggtca gataaccgaa acccagcgct
     1381 tcctgaatga ccttcccagg attatcggtg tggacgtgca ctttgatgaa attgctgtcg
     1441 tcggcgacca gaacacaatc tccgattgat tcgagatagg cccgaagcga caaaggatct
     1501 ttggcgaaag cggtagattt gttgataata aactcggtac aataagtgaa attgatttcc
     1561 tcgtcgtcaa cctcacccgc ggcaccggaa atttcagcgg ccgtttgagc cggctcctcg
     1621 gcctgaagga tgccattgcc gcttaaagta tagagcatgg cgtcgaatat aatgacgagc
     1681 ccttttccgc cagcatccac cacgcctgcc tttttcagaa ccggcagtag ctccggggta
     1741 ttggcaagcg cctgttttgc gctgttgaca gcagcctgaa agacggccag agctccaccg
     1801 ccctggctgg aagccctggc agcctcctga gccgattctc ttgccacggt cagaatggtg
     1861 ccttcggcag gtttcataac ggccttgtaa gccgaatcca cacctgcctg aagggcggca
     1921 gccagatcgg tgcctgaagc ctcggcttta tctttaagcc ctttggcgat tccccggaaa
     1981 agcagtgaaa ggatgacgcc ggaattgccg cgcgcccctc gtaaaagcga tgtggctgcg
     2041 agatccgcga cttcggtcac ggtctgggcc gacagcttgg aaagctcgcg catggcggag
     2101 cccatggtca tcgacatatt ggtgcccgtg tcgccgtccg gtacgggaaa aacatttaat
     2161 tcgttaacct cttgcttctg tgttccgatt gcgttcgagg cagagatcag catatcttta
     2221 agcagctggc ctgaaattgt ttccattcaa ctcttgcctt cctttataca ttcaaaatag
     2281 tgcggttgat ttccggcgga atgtcccaca gagtggatgc gtaaagaagc gatcctgatt
     2341 taatcggttt tcattccgtc gacaaaaaca tttacactgt caaccctgag gccggttgcc
     2401 tcctctaccg tatagcggac ttttttaata atgcttttta cgatagcggt aatattaact
     2461 ccataggtaa cgacaatatg aagatccaca accagacttt tgcccaggga acgtactttg
     2521 acaccgcggt caatcttatc ctgcccgaaa atggaggcga ggccttgagc tgtgctgctg
     2581 accgccatgc cggctacacc gtaacaggaa gacgcggcat atccgatcaa attggaaaaa
     2641 tattcatggt taacctcaat caggccgttt ggattttcaa accggatcat tcagttcact
     2701 ccttccggga actctgcgtt ttatcctaat catattttat cttattttta acagcttcac
     2761 aatgattatt tacgactttc ggatcgtaaa tccggagttc ttttgccgaa cagcgcagcg
     2821 ggcgtcaaaa gtgccaggtt tcgatattat aaaacaagtt gccgtcggtg gaaacaaccc
     2881 cggacaggcc ggtgtgataa gaaacggcgc ccgttttata gcaaaggggg ataaatggca
     2941 tttcgttttc aaatgccgcc gcgaatgcgt caagtgtccc atttccgttt cgccagttgt
     3001 tataggcggt cagagtagca gttttctgtc caattccgta ggatgacccg ccagactgga
     3061 gaaaggacga aagatccatg tcggagcgga gtttgacttc cccaagataa agatcgaaat
     3121 tgccgctggt gatccggctg ttgtatacct gggaagtaac cttttccacc gatatgttga
     3181 gccctgcctt gcgcaggttc gataccagat aatccgctgc aagagccctg acggaattat
     3241 cctggttaac cagcagcgcc agatccaggg agcttgttga gccaaaagat ttcagttcgt
     3301 tcttaaagct gtccgagtcg gatttcaact ttgatttatc cgtgcttttc aaaccggtca
     3361 gattcggata aagcggtaag atggatgaga gcgttcgacc ggaataaatt tctgttacga
     3421 gcgaatcgcg attcaccgca taggagaccg cctttctcag gtggacgtta ttaagcggcg
     3481 atcgggtcga attaacaccg ataaagatca gctgattcat gcttaccggt tttgacgata
     3541 cgttgacact cgactgcatc cctgatccat aatcggaatg taaaaaatcg atgtttccga
     3601 tttccagact ggacatcaag gcgtttttat ccggaatatt tacaagccgg atggttttaa
     3661 gattaggcgt tgtctttttg taccagaggg aattggcagt caaagtgatt acatccgttc
     3721 cgctgctcgc cagtttatag attccagacc cggtcggagt gtcactcgtt cccgtctcct
     3781 gtttgagaat gggcagattg agcagatttg tgagtagcgg atcggaagtt ttcaaattaa
     3841 aaataatatc gttgccgctg gtttcaatat ttgctatgtt ggaaacccga gggtaaaagt
     3901 aaccgccggt atttcccatc gcaagctcga agctgtattt gacatccgct gcagtgagcg
     3961 gagaaccgtc gctgaataca acgccgctgc gcagtgtaac ggtgaaaacg ttgttttggc
     4021 tgcttaccgc agaagccagc cgaagcaccg ggttaaaatt ttgatccggc tcggccagac
     4081 aatcatagaa caagggccac agctccagat tggtcatgga gtcagttgtg tagggattga
     4141 aagatttatt ggatacacag ggcagcgaga gggtggtgga gccggttacc gctttcgaag
     4201 tgacttttga tgtttgggat gatgaattat tttttgattc ttttttaccg cagccggcaa
     4261 aacatagcaa aaatgccagt atgacgacta gagctgtctg ataaggccgc ttcaaacgaa
     4321 tccctctttt cgcagggttg tcgatcattt ttaatatttc acaaaccgtt cggcctgtta
     4381 aataaacaca ttctatcata tcacgaacgt caatttaggg caagccgtag gggtataaaa
     4441 aacgggggcg aacgtcaagt gttcgccccg cgcccctctc gaacggaatt ccgtcagata
     4501 acttttacaa tcagccgaat tgccgtacgg ctttcgccac cgacctcaac atttgcaaat
     4561 ccgggtatgc agacaatgtc aatacccgag ggggcaagat aaccgcgcgc aatggcaatg
     4621 gattttatag cctggttggt tgctccggcg cctacagatt gaatttctgc agcattttta
     4681 tctctcacaa cattcgcgat tgcaccggcc acagcgtgag gtgctgactt ggacgagact
     4741 ttaatgattt ccatgaattt cccctcctgg tttttcaggt agtattgaat ccctttcctg
     4801 taatatattt tagaacgata caaagatatt ttcaagctat atttacagta tttgttaacg
     4861 aatttgcaac cttttcataa aatttgttcg ccttgttttg taatctatgt caaaaatgac
     4921 acctgagagc aaacaaggac cttcggcgtt ggaaaatctt accggcattt tagtgagaaa
     4981 ccttttaatt acatttttag aatcaacgcc caaaaccgac cggctgggcc ctgtcattcc
     5041 cacgtcggtg agatagcctg ttccgccggg aaaaatttct tcgtcagcgg tctggacatg
     5101 ggtgtgagtt ccaaagattg cggagacgcg gccgtcgaga taaaaagcga gggcccgttt
     5161 ttccgatgta gcctccgcat gaaaatcgac gataataatg ggattgccgt cgataccggc
     5221 gagaatatgg tcaattgcct taaaagggtt gtcaaaatta tcaataaaaa ctgtgccgat
     5281 gaggttgacg acacagatgg atatcttccc ggtatcaacc acacaaactc cattgccggg
     5341 cgctccttcc ggataattgg cggggcggat gatgcaggaa ttttcttcta ggtaattaaa
     5401 gacctcccgg cgtctgaaaa catggttccc ggttgtgata acatctacgc cgcttgtgaa
     5461 aagatgctcg gcggatgcag gcgtaattcc gtttccctct gccgagtttt ctccgttggc
     5521 aattacaaaa tcgatctggt ttattttttt cagagcctgc agatgctcac gcaggaagtc
     5581 gcatcccgct tttccgacaa cgtctccgat ggcaagaata ttcatgataa cctccaatga
     5641 aaggaattgt gcaggaaatt cagtatgaag cgtaagaaag tgataagaat agtataaccg
     5701 cgataattgc gatgttttag tggttttagg ggcagatttt taatttatcc ggagagtaac
     5761 aagaaagggt tgcgtaatta aattacgcaa ccctctgtat tcctattttg cgtattcgat
     5821 cgctctgctt tctcgaatca catgaacctt aatttgaccg ggataatcaa gctcgtcttc
     5881 gattttccca acgatatccc tcgccatcag tgtgattttt ccatcgtcaa cgatttcagg
     5941 cttgaccata attcgtattt cgcggcctgc ctgaatggca aaggattttt caacgccgtc
     6001 aaaagaattc gcgatatctt cgagtttctc cagtcgccgg atataatttt caagattttc
     6061 acgtcttgct cccggtctcg ctgcggatat ggcatccgcg gcctgaacca gacaagcgat
     6121 tacggtgtgc gcttccacat cgccgtgatg agcctcgatt gcatgaatga tatccgcatg
     6181 ctctttatat ttcttcgcaa tgtctacgcc gatttccaca tgagagcctt ccacttcgtg
     6241 gtcgaccgct tttccgatgt catgcagcaa tcctgccctt cgggcggtag aaacgtcaac
     6301 accaagctcg gctgccatca cacccgacag gtgggatact tcgagagaat gattcagaac
     6361 gttctggcca tagcttgtgc ggtatcttaa ccggccgaga agttttacaa gttcatgatg
     6421 aataccatgg attccagctt cgatgacggc tctttcgccc tcctgccgga tggtgttgtc
     6481 gacttccttg cgggcttttt cgaccatttc ctcgatccgc gcaggatgga tacggccatc
     6541 tgagatgagc cgctcaagtg aaatccgcgc aacctcgcgc cggacgggat caaaactgga
     6601 caacgtaatc gcctcaggcg tatcatcgat aatcagatcg acgccggtca gcgtttccag
     6661 tgtccgaata ttgcggcctt cacggccgat aattcttccc ttcatctcgt cattgggaag
     6721 aggcactacc gatacggttg cttccgctac atgctcggcg gcgcattttt gaaccgcgag
     6781 cgagatgatt tcgcgggcct ttttctcgga gtcttcctta agctgctgct catattcttt
     6841 gatttttacc gatttttcat aggtaagctc attttccaga ttggtcagaa ggaagtcttt
     6901 tgcctgttcc acggtgaaac cggaaattcg ttcgagcatt tcaaactggc gttttttaac
     6961 ttcttccgct tcgtgcagtc tctcttccga ttccttgttc tttttattca ggttttcgtc
     7021 tttgacttca agctgctcga ttttgcggtc aagcgtttct tctttttgct ggagtctgcg
     7081 ttcctgtctc tgaacgtcca cgcggcgttc ttttatttct ttttcagctt cggttctcat
     7141 ccgatgaata taatcttttg cttccaaaat aacttctttt ttcttgctgt ccgccgattt
     7201 cagggcttcg cttacgatac gtttcgactc ttcttccgct gagcccagtt ctgcttcggc
     7261 aacccgtttt ctataagcag cgccaagaaa aaaggcgaga ataccgaaaa cgatcgcggc
     7321 tgcgaccgcc gctataacac ccactacaac ataggttccc attattgcct gaaggcacct
     7381 ccttataatt cagaatccac attagactgc cgcataaact tacgcatatt tcgcatattc
     7441 aatttttttg attgagttgc tatatatacc aattatgcaa agaagtctgc atgtaaaagc
     7501 caaatatatt aaaaattatt actatattac tatattctaa agccattgtg aaataatgtc
     7561 aagcaaaagg tttcaatatc caggcggcgc cgcagttgac atccgaccct aaaatatgat
     7621 agaatttctt aaaagcgttg aaggggaatt agaaggcgca gggtttgctg aaagagagga
     7681 tcgccacggg ctgaaagcga ttcgcatacc gtccttcgaa aaccaccccg gagcgtgcgc
     7741 tgaagccgag aggataaagc gcgacgggcg gctccgttaa aagcccgaaa gcggcgttgg
     7801 gcaaaatacc cggcgcaatt tgggtggaac agtggggaat ttcctcaccc cttatctgga
     7861 aggggtgggg cttttttatt gttagaagga aaaatgacgg aatatttttt cagtcggaat
     7921 tccgtcagaa ttgcaggatt cgtgcggctt tggtgcgaaa gtaaaaaaaa taatttgcaa
     7981 gcgagggtgg aaaaatgatt caaatcacat taaaaggcgg agtcgtcagg gaatatgagc
     8041 cgggcgtaac tcccatggaa atcgccaagt cacttggcgc aggtctttat aaggctgcct
     8101 gcgcagcaaa aatagacggc aggcttgctg atttaagaac tccggtgacg gccgacagcg
     8161 ctgtagagat tctgacgttc gcggaagaag aaggaaaaaa gactttttgg cacacggcct
     8221 cccatatcct ggctcaggcc gtcaagcgtc tttatcccaa cgttaaactg accatcggtc
     8281 ccgccattga caatgggttt tattacgatt tcgactcgga aatcgcgttt tcggcggaag
     8341 aacttgaaaa aatcgaagcc gaaatgaaaa aaatcgtaaa ggaaggactg cagcttgagc
     8401 gttttgagct cagcgccgac gaagcggtcc gtcttatgga ggagaggagc gagccctata
     8461 aaatcgaact gatcggggag catgcct
//
LOCUS       DATALY010000114        14381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_802363,
            whole genome shotgun sequence.
ACCESSION   DATALY010000114 DATALY010000000
VERSION     DATALY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802363"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VHR42_03340"
     CDS             <1..956
                     /locus_tag="VHR42_03340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX3026247.1"
                     /translation="SALEALTCIKESGIRLKRPVEMVALIKEEATEFLGGLFGSKAIC
                     GMLPDNYADITRDKSTGRLLRDAMKEFGMGLDPDRIRASMRGREEFYRFFELHIEQAG
                     LLEERDLPVGIVTTIAGIHQMKFELTGRSAHAGAMPMEQRKDAMAAAASIACEVERLA
                     KNSGSETRATVGYISAYPGEHNIIAGNATIPVDIREKNPVVRKRIFDSLLQCVEQECR
                     SRGVKSEIQLTLDSPPVNCDPCIVNLFENAAKQQGVPFMEIVSYAAHDAMNMATLCPM
                     GMIFVRSKDGLSHCPEEFTSKEDLAAGANVLLNSMIQAASE"
     gene            1045..2475
                     /locus_tag="VHR42_03345"
     CDS             1045..2475
                     /locus_tag="VHR42_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3026248.1"
                     /translation="MLDILILHGFVITMEGRGLGIIEDGAVGIKGNKIEVVGVSGEIQ
                     KEYSAHRVIDAENKVVMPGLIDAHTHTGIGLIRGVSQDLKNLMQQGIWPFEELLRKDV
                     DASVKGSAVNIIEAVKAGTTTFCDYDYPMSEIVKNHEKIGTRARVADLINELPPDSSK
                     VKVGDLYPLDPASGNRKLQENIRLIEEWNGRDDGRITCMLGPQGPDMVSKELLLEIRS
                     LAEKYDTQIHMHVAQGNREINQMVKRYGKRSIAYLDEIGMLNSRLLAVHLTEATPEET
                     RTVARSGAAMILCSGSIGIIDGLIPPAAEFLEVSDRLALGSDQTPGNNCSNMFNEMKF
                     TAILNKCKARTPDVFPAHQVLKMATIKAARAIGLGDEIGSLKEGKKADVILIDLLSPN
                     LSPVIHTPVRNIIPNLVYSAKGSEVETVIIDGKVVVDNKVLTTVDERKAVLEANAAAK
                     RIADQAAAKFATMETPLKDWMAEGNL"
     gene            2578..3810
                     /locus_tag="VHR42_03350"
     CDS             2578..3810
                     /locus_tag="VHR42_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3026249.1"
                     /translation="MNTSKQRIKNLVSQQEALNIVKDIVMIPSHQGYPKREKPVAEYL
                     LKLFESEGIEAYLQEVYPGRPNVVAKIPGTGRGRSLMFNGHIDTVPPNGMDHPFDGMI
                     KDGKLYGRGSADMKSGVGTMAYAMILLKRAGVKLKGDLIYIGVIDEDAAGSAGTRYIV
                     KNGPLTDFAIVGEPTSLEPVIAHKGIDYFEVTFHGKSVHSSVPENGVNAILAAGEFIS
                     RVENELMPEYSAKIHHLCGAPTINVGLIQGSAKANAQYLLGKVETFAGIIPDVCKVFI
                     DVRWTPDQTIEKVERDIRNIACKVESNREGISAETVYIPMPRPAMEIDADNELVRSII
                     SNSKETLGKECRVKGEKYWGDSGLLYGISKIPTIMYGPGDIGCAHADIEWVETEQLAK
                     AAEVYAMTAIDICGVEEE"
     gene            3832..5229
                     /gene="allB"
                     /locus_tag="VHR42_03355"
     CDS             3832..5229
                     /gene="allB"
                     /locus_tag="VHR42_03355"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX3026250.1"
                     /translation="MSGKKTDILLKNALVVLESGAVKANLAVKDGKTAGILDPSVEVE
                     ADEIRDLNGKIIFPGLIDTHAHYWEPGPFNYREDFYHATRVCAAGGITTTIEMPLSVP
                     PVVDKKTFEMKYEIAKKNSVVDFALWGGLIPTSVDQIEEMNEIGCCAFKAFISFASPE
                     YPRIPDHDLCRAMEITSKFGGLIGVHAENAEIVDDFCRRKEAEGIRQPSRYHEGRPPF
                     SELEAIERATLFARQYNARVLICHMSAVEGFEATNRERACGRPVYTETCPHYLAFDNT
                     KMDEIGPFAKCNPPLRPAENREKLWEHIRCGDIDVIGTDHGPYSKEEKAAASDNIWQA
                     LCGFGGADMDLPVLLTEGYHKRGITLERIAKISSINAARIFGLKSKGAIEVGNDADYA
                     IVDLNSEWVYDGMKTFSKTKLDCGPYQGYRAKGRILETMVRGKTVYRDGEIKVDSGYG
                     KFISGTKMPKGCEKE"
     gene            5235..6473
                     /locus_tag="VHR42_03360"
     CDS             5235..6473
                     /locus_tag="VHR42_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3026251.1"
                     /translation="MTDLEKKVLSEVDEKEVVEFLQKLIRCRSDYPPGDTREVAEACR
                     RKFEEAGIPAELIYPHPDTFTIWNDHVDVTKIPSVIGTYQGDKEGPVLLYNAHIDTVP
                     GDNIKNWRNDPFSGIIEDGKYIYGRGAGDDKGSVAAQVMAAVILARAGIKLKGTLQIS
                     PVADEEASSYRGTKFLRDEGYLKPDIVVIGEQTDNKIAVAERGVLWMKITIIGKGAHA
                     AMPWAGNNAVARAAEFVNLINTEYADELEKRTNPYLPHATASVTGFNGGIKDNVIPEL
                     CTLTIDRRLVPGETVEMAVGEIKNLLKKAAADHSFEWDVRVTYDAGPPVNTQPNDPLI
                     QSMLKIKEELTGAPDQPIGYRQGSDGRLFTHLGIPIAIYGPSDPAVGHAPNERVSIEQ
                     LLEATRVYILIAMRILGCIS"
     gene            6574..7929
                     /locus_tag="VHR42_03365"
     CDS             6574..7929
                     /locus_tag="VHR42_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3026252.1"
                     /translation="MGEKTLTRKLGFWAALAIGVGSTVGSGIFVNCSTVAKAGGSPFL
                     SILSWVVGGLVMIPQMMILAELSTAFPDNGYGYVLFSRAGSKPLAFLFGWATFWALDP
                     TGIAVLALASVTYIAFFIPVLTGFFGNLLATVLIIVFAVLHYRSVKTGGAFTVMITIA
                     KILPFALIIGLGLFFIHGGNFSNAAVATKIPFGSGLMAGVSATTWAYSGMAGICYMAG
                     EFKNPGKTLPRALIGSAIVIMALYSFVSAAVHGLLPFDKIIATSTPITDALRTIPFFS
                     SFAPAFVAFTAILVILGCLNTSIMFEPRMEFAMAQDNLFFKVFDHVHPKYETPDRSIA
                     LVVGFAIVQVWATNIQTLLGYTTLVLLIMNTLLYLTIITCRKREDYKPVFHCPAWPVM
                     MVLSSAACAWMAWGTFQWAPIPGIIAAAAVVVTGLPAYYYWNSKKKKLENSETKEKVS
                     V"
     gene            8020..9003
                     /locus_tag="VHR42_03370"
     CDS             8020..9003
                     /locus_tag="VHR42_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX3026253.1"
                     /translation="MKTIVTFGEVMLRLQSPDYKRIQQAGSFDVYYGGCEANVAVALS
                     QMGEKAAFVTKLPENPLGDACRNEIRKWGVDTSMIVRGAGRMGIYFTEKGASQRPSSI
                     LYDRSSSAFSLASPENFDFDRIFNDAEWFHFSGITPALSESAREITLTAIRKAQEKGL
                     KVSCDFNFRSLLWSKQQARKTMSELIREIDLLIINENQAAEVLGIREEDYEKTSEKIA
                     DSFGIKKVALTKRRTDSGEVNRFSAMLRCGPETAWSKEYSIFMIDKIGGGDAFSAGLI
                     YALCHGENPENAVEFAAASACLKHTIEGDFTVAGVEEIRQLMRNGSARFVR"
     gene            9102..9752
                     /locus_tag="VHR42_03375"
     CDS             9102..9752
                     /locus_tag="VHR42_03375"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEX3026254.1"
                     /translation="MNLKKYEIIKEIQETGLVAVIRAESAEQAAKSADAIVQGGIRIL
                     ELTLTVPGAIDVISSLKQRYKGKNVTIGAGTVLDPETARLCILTGAEFIVAPSLNVDT
                     IRLCNRYGVPIVPGGTTITEVVTALEYGADIVKLTPASAFEPSVIPAIRGPVPQAQFM
                     VTGGISPENAGDWIRAGAVAVGAGGELTRGAKKGDYTLVTQTAEAYVREIQKARGK"
     gene            complement(9843..10007)
                     /locus_tag="VHR42_03380"
     CDS             complement(9843..10007)
                     /locus_tag="VHR42_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026255.1"
                     /translation="MVHKPKELVQDIETLASPYSTRLCVTDVCLIADSAGFSKNNTGH
                     QTVETICPSR"
     gene            10591..10944
                     /locus_tag="VHR42_03385"
     CDS             10591..10944
                     /locus_tag="VHR42_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026256.1"
                     /translation="MTYRAIFPEIYYKLKPYISMTCDLIFSYGMMPTQQQLEEASDGI
                     YNDFCAMYPDMAEYMSKSGGKDDPPGDPPFRGGLREGFRPGFGFSRFRRRGLGRDFID
                     ALLLSELLGRGGFLF"
     gene            11106..12029
                     /locus_tag="VHR42_03390"
     CDS             11106..12029
                     /locus_tag="VHR42_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HEX3026257.1"
                     /translation="MLTGNEFVKQSLALHLFFARIMKEHSFFLEVGFTPKDYNFTNQA
                     DSFRKAFDALLADAVALSNGVVNQEVLQSGEVITPYTLKAETVSAYFTGVRIQTELTQ
                     SEASLAGDGMSNSSMLEQRVYYLNDNAIKLINSLAQFKTTILSNVISCRMFTVNYPLL
                     IDHILREAKFYLMLVQKLQNREDFNLEKEVYEQETFWNRIMAEHSKFIRGLLDPTENE
                     LMITANNFGNEFDQLTNQAKQAMDKTIPLKKVTTDSLSATMKIRDFKTAGTEGILNCK
                     IKSIIIPLLGDHVLREANHYLRLLNMFEKRI"
     gene            12305..12511
                     /locus_tag="VHR42_03395"
     CDS             12305..12511
                     /locus_tag="VHR42_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX3026258.1"
                     /translation="MDRFALILVIIGAIVWGVVGIFGMNPVAWIMHGSMTVLSRIIYT
                     LIGLGGLWCIKLLFRERELVQHHE"
     gene            complement(12568..14112)
                     /locus_tag="VHR42_03400"
     CDS             complement(12568..14112)
                     /locus_tag="VHR42_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3026259.1"
                     /translation="MSERTLSYWNTTAEIAEYPRLSESLETDILIIGSGITGITCAYC
                     LAQKGAKPVVIEAGGLCDGTTGNTTGKITIQHDIIYSHILEKHGIDDAKLYAESQTSA
                     VDFVREQVKKESIDCQLTNSTSYIYASSQEDRDMVVREYEVAKEIGINAEFYEKSNFP
                     PDNFGMVGFKNQAIFHSVRYVNALAKAAASKGVRIYCDTKAIKVDDDDIKTVYCENDI
                     TIKAKHVVMATQYPIYDGPNIFYTRLYAKRAYGIAVSPEKDWPDGSYINTGDPTRSIR
                     THVENGKRILIVVGENHATARGEDNTELHYNNLIQFAKHIAGTNDLLAKWSAQDYDTP
                     DQIPYIGRMSDYSNLYIATGFGKWGLSNGTLAGNMIADLITSGNCRYEELYSRKRGDY
                     SSSAGKTVSEVMGSVGELIKSKLERTESVRNLKPYEGRVINFDGQKAGIYRDFDDNVT
                     VLDISCTHMTTELNFNAAEKTWDCPAHGGRFSASSGKLLEGPPKDSLQILYQGKFSDL
                     VVEKGR"
     gene            14221..14381
                     /locus_tag="VHR42_03405"
                     /pseudo
     CDS             14221..14381
                     /locus_tag="VHR42_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059252.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
BASE COUNT         3878 a         3163 c         3758 g         3582 t
ORIGIN      
        1 tgtcggctct cgaagcgctt acctgcatca aggagtcggg catccgttta aaacgccctg
       61 tcgaaatggt tgcgcttatc aaagaagaag ccaccgagtt tctgggtgga ttattcggga
      121 gcaaagcgat ctgcggcatg ctgcccgaca actatgccga tatcacgagg gataaatcaa
      181 ccggccgact tctgcgggat gcaatgaaag aatttggaat ggggctcgac ccagacagga
      241 ttcgggcatc tatgcggggc agagaggaat tttaccggtt ttttgaactg cacatcgaac
      301 aggcgggatt actcgaagaa cgcgatctgc ctgtgggaat tgtcacgact atcgccggta
      361 tccatcagat gaaatttgag ctgaccgggc gttccgcaca tgccggagca atgccgatgg
      421 agcagcgcaa agatgcgatg gcggccgcgg ccagcatcgc gtgcgaggtt gaaaggcttg
      481 cgaaaaattc cggttccgag accagggcga cggtcggtta tatatcggcc tacccgggtg
      541 aacataacat aatcgccggg aatgcgacga tcccggtgga tatccgggaa aagaatccag
      601 tggtgagaaa acgaatcttt gattcgctcc tgcaatgtgt cgagcaggaa tgccggtccc
      661 gaggggttaa aagtgaaatt cagctgactc tggattcacc gcccgtaaat tgcgacccat
      721 gtatcgtgaa cctttttgaa aatgcggcga aacagcaggg cgtgcctttt atggaaatcg
      781 tcagctacgc tgctcatgac gctatgaata tggcgaccct ttgcccgatg ggtatgattt
      841 tcgtacgcag taaagatggc ctgagccatt gcccggaaga attcacttca aaggaagact
      901 tggcagccgg cgcaaacgtg ctgctaaact ctatgataca ggcggcatcg gaataaaccg
      961 ggcctgcgat gtttgaggca attcacgccg cctttaaaaa aaggacagca cgcggcggcg
     1021 aatcagaaga aaagggtgcg aatgatgtta gatattttaa tcctgcatgg atttgtaatt
     1081 accatggagg gcaggggcct tggcattatc gaggacggcg ctgtgggaat caagggaaat
     1141 aaaattgaag tcgtcggcgt ttccggtgaa attcagaagg aatattccgc ccacagggtt
     1201 attgacgcgg aaaataaagt cgttatgccg ggtttgattg acgcacatac tcataccggt
     1261 attggtctga tccggggcgt gtcgcaggat ttaaaaaatt tgatgcagca aggaatctgg
     1321 ccgtttgagg agctgctgag aaaagatgtt gacgccagtg taaagggctc tgcggtcaat
     1381 attatcgaag cggtcaaggc cggtacgaca acattctgcg attatgatta cccaatgagt
     1441 gaaattgtaa aaaatcatga aaaaatcgga acaagagcgc gggttgccga tctgatcaat
     1501 gagcttcctc ccgacagcag caaagttaag gtcggagacc tgtatccgct tgacccggcg
     1561 tcgggcaaca ggaaacttca ggaaaacatt cggctgatcg aagaatggaa cggccgcgac
     1621 gacggcagaa tcacctgtat gcttggcccg cagggacctg atatggtgtc aaaagaattg
     1681 ctgctggaaa ttcggtcact tgcggaaaaa tacgacacac aaatccatat gcatgtggct
     1741 cagggcaatc gtgaaattaa tcagatggta aagcgctatg gaaaacgaag catagcatat
     1801 ttggatgaaa tcggaatgct taattcccgg cttttagcgg tgcacttaac ggaagcgacg
     1861 ccggaagaga ccagaacggt tgcaagatcg ggtgcggcaa tgatcctttg ttcgggaagc
     1921 atcggaatta tagatggtct gatcccgcca gccgcggaat tcctcgaagt aagtgacagg
     1981 cttgccctcg gttccgacca gacgcccggc aacaattgca gcaatatgtt taatgagatg
     2041 aaattcacag caattttaaa taaatgcaaa gcaagaaccc cggacgtatt ccctgcccac
     2101 caagttttga agatggcaac gatcaaagca gcacgtgcca ttggtctcgg agatgagatc
     2161 ggttcgctga aagaaggcaa aaaagccgat gtgattctca ttgatttgct gagcccgaat
     2221 ctttctcccg ttattcatac gccggtaaga aatatcatcc cgaacctcgt gtattcggca
     2281 aaaggcagcg aggtggaaac cgttattatt gacggtaaag tggtggtgga caacaaagtt
     2341 ttaacgacag tggacgagag aaaggcagtt cttgaagcaa acgcggcggc aaaaaggatt
     2401 gccgatcagg ctgcagcgaa gtttgcaaca atggagaccc cgcttaagga ttggatggcg
     2461 gaagggaatc tttgattccg caattactga aacagatttg ttatcgtgca aacgcggcgg
     2521 ttcaagcgtt tcgaataaag cgatccgggc cgtcgcggac ataggaggaa actattcttg
     2581 aatacaagta aacaacgcat taaaaatctg gtaagccaac aggaagcgct gaatatcgta
     2641 aaggatatcg tgatgattcc gagtcatcag ggatatccga agagagaaaa accggttgcg
     2701 gagtacttgc tgaagctgtt tgaatccgag gggatagaag cgtatctgca ggaggtttat
     2761 cccggcaggc ccaatgtcgt ggccaaaatt ccgggaaccg gcagaggcag aagcctgatg
     2821 ttcaacggtc atatcgacac ggttccgccc aatgggatgg atcatccgtt cgacggaatg
     2881 atcaaagacg gcaaacttta cggaagaggt tccgcagata tgaaaagcgg agtcggcaca
     2941 atggcctacg caatgattct gctcaaacgc gccggtgtaa aactcaaggg tgatctgatt
     3001 tatatcggcg ttattgacga agacgcggct ggaagcgccg gaacccgtta catcgtaaaa
     3061 aacgggccgc tgaccgattt tgccattgtc ggcgagccga cttctctgga accggtaatc
     3121 gcccataagg gtatcgatta tttcgaggta acgtttcacg gcaaatcagt tcactccagc
     3181 gtccctgaaa acggcgtcaa cgcaattctt gccgccgggg aatttatcag tcgggtcgaa
     3241 aacgaactga tgccggaata ttccgcgaaa atccatcatc tgtgcggagc gcctacgatt
     3301 aacgtcggtc ttatacaagg ctccgcgaag gctaatgcgc agtatctgct tggcaaagta
     3361 gaaacttttg cgggaatcat acccgatgtc tgcaaagtat tcatcgatgt ccgctggact
     3421 cccgatcaga ccattgagaa agtcgaacgg gatatccgga atattgcctg caaagtggaa
     3481 agcaaccgtg agggtatcag tgcggaaacg gtatatattc ccatgccgag acctgcgatg
     3541 gaaatcgacg cggataatga gctggttcgt tccattatct ccaacagcaa agaaactctt
     3601 ggtaaggaat gtcgcgtcaa gggtgaaaaa tactggggcg actccggtct tctttacgga
     3661 atttcaaaga ttccgaccat tatgtatggc cccggcgaca tcggctgcgc ccatgctgat
     3721 attgagtggg ttgagacgga gcaattggca aaggcggccg aggtttacgc gatgacggcg
     3781 attgatatct gcggtgtgga agaagaatag gcagaataag gagggatttc tatgtcaggg
     3841 aagaaaacgg acattttgct gaaaaatgct cttgttgtat tggaatccgg cgcggtaaaa
     3901 gccaaccttg cggttaagga tggaaagact gcaggaattt tagatccttc ggttgaggtc
     3961 gaggcggatg aaatccggga tctgaacgga aaaatcattt ttccgggtct gattgataca
     4021 cacgctcact attgggaacc cggcccgttt aattaccgtg aagattttta tcatgcaacc
     4081 agagtatgcg cggcgggagg tattacaaca acaattgaaa tgcccctgag cgtgccgccg
     4141 gttgtagaca aaaaaacatt tgagatgaaa tatgagattg caaaaaagaa ttcggtggta
     4201 gacttcgccc tctggggcgg gttgattccg acctctgtgg atcagattga ggaaatgaac
     4261 gagatcggct gctgcgcttt taaggccttt atttcatttg caagcccgga atatccccgt
     4321 atacccgatc atgatctttg cagggcaatg gagatcactt caaagttcgg cggactgatc
     4381 ggcgtacacg ccgagaatgc tgaaattgtc gatgatttct gccgcaggaa ggaagcggag
     4441 ggaattcgtc agccttcgcg gtatcacgag gggcgtccgc cgttttccga actggaagca
     4501 atcgagcgcg caacgctgtt tgccagacag tataacgcga gggttctgat ctgccatatg
     4561 tcggctgtgg aagggtttga ggcaaccaat cgtgagcgtg cctgcggaag accggtttat
     4621 acggaaacct gcccgcatta tcttgcgttt gacaatacga agatggacga aatcggtccc
     4681 tttgcaaaat gtaatccgcc gctgcggccg gcggagaacc gtgaaaaact gtgggaacat
     4741 atccgctgcg gagatatcga tgttatcgga accgatcacg gcccatactc caaagaggaa
     4801 aaagcggctg caagcgataa catctggcag gcattatgcg gattcggagg agcggacatg
     4861 gatctgccgg tcttgctgac cgagggctac cacaaacgag gaattaccct cgagcgtatt
     4921 gcgaaaatca gctccatcaa tgccgcaaga attttcggcc tgaagtcaaa aggcgcgata
     4981 gaagtcggca acgatgcgga ttatgccata gtcgacctca acagcgaatg ggtttatgac
     5041 ggtatgaaaa cattctccaa aacaaagctg gactgcggcc cttatcaggg ctaccgcgcg
     5101 aagggcagga ttcttgaaac gatggtaaga ggtaaaacgg tctaccgtga cggtgaaata
     5161 aaagttgatt caggttacgg aaaatttatt tcgggaacca aaatgccgaa aggctgcgaa
     5221 aaggagtgat ctgtttgacc gacttggaaa aaaaggtgct ctcggaggtt gatgaaaaag
     5281 aagtcgtgga attccttcag aagcttatcc gctgcagaag cgactacccg ccgggagaca
     5341 cgcgcgaagt ggcggaagca tgcaggagaa aattcgagga ggccggaatt cccgcggaac
     5401 ttatctatcc gcaccctgac accttcacga tttggaacga tcatgttgat gttaccaaaa
     5461 ttcccagtgt gatcggaact tatcaggggg ataaagaagg gcccgtcctg ctgtataacg
     5521 cacatatcga cacagtgccg ggagacaata ttaaaaactg gcgcaatgat cctttttccg
     5581 gcatcattga agacggaaaa tacatctatg gcaggggtgc cggagacgac aagggttctg
     5641 tggcggcgca ggttatggcc gcggtgattc tggcaagagc cggcatcaaa ctcaaaggca
     5701 cgctgcagat cagtccggtg gctgatgagg aggcctccag ttaccgcgga acgaaatttc
     5761 ttcgtgacga gggctatctt aaaccggata tcgttgtgat aggggaacag accgataata
     5821 aaatcgcagt tgcagagcgc ggcgtattgt ggatgaaaat cacaatcata ggaaaaggcg
     5881 cccacgccgc gatgccctgg gccggaaata acgcggtggc gagagctgcg gaatttgtaa
     5941 atttaatcaa taccgaatat gcggatgagc ttgaaaagag gacgaatccc tatctgcctc
     6001 atgcgacagc cagcgtgacc ggattcaacg gtgggatcaa agacaatgtg attccggagc
     6061 tttgcacttt gacgatagac cgtagattgg ttccgggtga aacagtggaa atggctgtcg
     6121 gagagatcaa gaatctgctc aaaaaggctg cggcggatca ctccttcgaa tgggatgtcc
     6181 gagtgactta cgacgctgga cctccggtga acactcagcc gaatgaccct ctcattcagt
     6241 cgatgttgaa aattaaagag gagctgacgg gggcgccgga tcaaccgatt ggataccggc
     6301 agggaagtga cggaagactt tttacccatc tggggattcc gattgcaatt tatggcccaa
     6361 gtgaccctgc ggtcggccat gcgcccaatg aaagggtttc aattgaacag ctgctggaag
     6421 caacacgggt ctatattcta atagccatga ggattcttgg ctgcatcagt taggcggtaa
     6481 agtagaatgg agggtgaaaa gaaaaaggtg agaaagcaga ccaaaagtct gagacgaatc
     6541 cggtatgatt gaatcaaatg gggaggttta ttaatgggag aaaaaacttt aacacgaaag
     6601 cttgggtttt gggctgcgct ggctatcggc gtaggttcaa cagttggttc cggtatcttt
     6661 gttaattgca gcacggttgc gaaagcgggc ggcagtccgt ttctgtcgat tttatcctgg
     6721 gtggtcggcg gtcttgtgat gattccccag atgatgattc tggcggaact ctccaccgct
     6781 ttcccggata atggatacgg ctatgttctg ttcagcagag ccggctccaa acctctggcg
     6841 tttctttttg gatgggcgac gttttgggcg ctggacccta ccggcatcgc tgttctggcg
     6901 cttgcttccg ttacttatat cgctttcttt attcctgttc ttaccggttt cttcggcaat
     6961 ttgctggcaa ctgttctcat tattgttttt gctgttttgc attaccgcag cgtaaaaaca
     7021 ggcggcgcgt ttacagttat gataacgatc gcaaaaattc tgccctttgc gctgatcata
     7081 ggtttgggat tgtttttcat tcatggcgga aatttctcaa atgcggccgt tgcaaccaag
     7141 attcccttcg gttccggttt aatggcgggc gtttccgcga ccacatgggc ttattccgga
     7201 atggccggaa tctgttatat ggcgggtgaa tttaaaaatc ccgggaaaac gcttccgcgt
     7261 gccctgatcg gctcggccat agtcatcatg gcgctctatt cctttgtttc ggccgccgtt
     7321 cacggattgc tgccgtttga caaaatcatc gcaacctcaa cgccgatcac agatgctctg
     7381 agaacaatac catttttctc aagttttgct cccgcgtttg ttgcgtttac cgcgatattg
     7441 gttattcttg ggtgcttgaa taccagcatt atgtttgagc cgagaatgga attcgccatg
     7501 gctcaggata acctgttctt caaggtgttt gaccatgtgc atcctaaata tgagaccccc
     7561 gatcgatcca ttgctcttgt tgtaggattt gctattgttc aggtatgggc taccaacatc
     7621 cagacgctgc taggttacac gacactggtt cttctgatta tgaataccct gctgtatctc
     7681 accatcatca cctgcagaaa aagagaggac tacaaaccgg ttttccattg cccggcctgg
     7741 cccgtaatga tggttctttc ctcagcagcg tgcgcatgga tggcgtgggg aactttccag
     7801 tgggccccga tccccgggat tattgccgcg gctgccgtgg tggtgaccgg acttcctgct
     7861 tattattact ggaatagcaa aaagaaaaaa ctagaaaata gcgaaacgaa agaaaaggtc
     7921 agcgtttgat cacttaccgc atcctaaaaa aatctaaaat cataattgta ttttcctgcc
     7981 acatcacggg ggacgaacgg ttgaaaggtt tggtcgaata tgaaaacaat cgtaactttt
     8041 ggtgaagtta tgttacggct ccagtcaccg gattataaaa ggatccagca agcgggaagt
     8101 tttgatgtgt attatggcgg ctgcgaggca aatgtcgccg tcgcgctttc tcagatgggc
     8161 gaaaaagccg catttgtcac aaagctgccg gaaaatccgc ttggagatgc ctgccggaac
     8221 gaaatcagaa aatggggcgt tgatacttcg atgattgtcc gaggagccgg tcggatggga
     8281 atttatttta cggaaaaggg agcgtcccag aggccgtcga gcattctgta cgaccgttca
     8341 tcttcagcat tttcgctggc atccccggaa aattttgatt ttgacagaat cttcaacgat
     8401 gcggaatggt tccatttttc ggggattacg ccggctttaa gcgaatctgc ccgcgagatt
     8461 actctaacgg ctattcggaa agcgcaggaa aaaggattga aggtatcgtg tgattttaat
     8521 ttccgttctc tgctttggag caaacagcag gcgcgcaaaa caatgtcgga attgattcgg
     8581 gaaattgact tgttgatcat caacgaaaat caggcggccg aggttctggg aatccgcgag
     8641 gaggactatg aaaagacttc ggaaaaaata gcagattcgt tcggtatcaa aaaagttgcg
     8701 ctgacaaaac gcagaacgga ttcgggtgaa gtaaaccgtt tttccgctat gcttcgatgt
     8761 gggcccgaaa cggcatggtc aaaggaatat tctatcttta tgattgataa gatcggggga
     8821 ggagatgcat tcagtgcagg tcttatctat gcgctgtgtc acggggaaaa tcctgaaaac
     8881 gcggtagaat tcgccgctgc gtcggcatgc ctgaaacata cgatagaagg cgactttacc
     8941 gttgctgggg tggaggagat tcggcagctt atgcggaacg ggagcgcgcg ttttgtccgt
     9001 taaagaacgc acggcagctt tctgacagat attttttctg ggttccttac ataacatttt
     9061 agaaaagaga ttcttcattc ttaataacag ataaggagtc ggtgaatttg aaaaaatatg
     9121 aaattataaa ggaaattcag gaaaccggac tggttgctgt gatccgtgcc gaatcggcgg
     9181 agcaggcggc gaaatcggcc gacgcgatcg ttcagggcgg tatccgaatt ttggaattga
     9241 ccttgactgt acccggagca atcgatgtca tttccagcct gaaacagcgt tataaaggca
     9301 agaacgtaac aataggcgcc ggcacggttc ttgatccgga aacggcgagg ctgtgtatcc
     9361 ttacaggagc ggaatttatt gtggccccgt cgctcaatgt cgacaccatt cggctttgta
     9421 accgatacgg agtgccgatt gttccgggcg gcacgacgat aaccgaggtg gttacagctc
     9481 tggaatacgg tgcggacata gtgaaattga caccggccag tgcatttgaa ccatctgtga
     9541 ttccggcgat aagaggcccg gttcctcagg cccaatttat ggtgacggga ggaatcagtc
     9601 ccgaaaatgc cggtgattgg atcagggcag gcgccgtagc ggtcggagcg ggaggggagc
     9661 ttacccgcgg ggcgaaaaaa ggcgattaca cacttgtaac tcagactgca gaggcctacg
     9721 tcagggaaat tcaaaaggca cgcggtaagt aaaaccagtt ttcatagcgg ttgattagtc
     9781 acgaacccaa agatgtcgca taagcctgag ggcttatgcg agatctttta ttatggcgcg
     9841 atttacctgc ttgggcagat ggtttccacc gtttggtggc ccgtgttatt ttttgaaaac
     9901 cctgcggaat cggcaatcag gcagacgtcc gtaacgcaga ggcgggtgga atatggtgat
     9961 gcaagtgttt caatatcctg cacaagctcc ttcggcttat gaaccattca atatgccggg
    10021 ccggaaacac ctttaaacat ataataatta tggaggtgat aattttgttc caatacgcga
    10081 tacagccggg ggataccatt tacagtattg ccgcacagtt tggagtctca attgacgcca
    10141 tattgtcggc aaaccctggt ttatatccct ataatttgat cgtcggccag atcattttaa
    10201 ttccaacggc cggctattac ccctatccca ggtatcccgt ataccccaga tatccggtgt
    10261 ttcctatctt ccccataccg ttcagacgtt ttccgaggcc tcgttttgaa cccgaaagac
    10321 ccggaagacc tgaaagacgc gaaagacccg gaatgtctgg aacacccgga acgccccgta
    10381 cacccgggat gccacgaacg cccggcacac gcagaccata gcagagggcc tgactataac
    10441 atatgaaata ttggaggcga cgacatgtcg agtcaagaaa gttcaaatat ggggccaatg
    10501 ggaagcacac ctatgaacaa tcaatccgtt atgcctgaaa tgatgcaatc ttatcccccg
    10561 acagctgtaa atccagctct ggaggatgtc atgacatatc gggcaatttt cccggaaatc
    10621 tattataaat tgaaaccata catttcaatg acctgtgatt tgattttctc ctatggtatg
    10681 atgccgacgc agcaacaatt agaagaggca tctgacggta tttacaatga tttctgcgca
    10741 atgtatccgg atatggccga atatatgagc aaatccggag gaaaagacga cccaccggga
    10801 gacccgccct tccgcggcgg attaagagaa ggattcagac cgggttttgg ctttagcaga
    10861 ttcagacgca gggggctcgg aagagatttt attgatgcgc tgttgctgtc cgagctcctc
    10921 ggcagaggcg gattcctatt ttaatcatgc ggcaaaacgg atcatgcttt ttgtagaaat
    10981 tagatcaatg aaagagggca tctttcaggt ggcttctttc attagttccg cagcaaacat
    11041 tccactatcc ttggttcatg ttatgaagcg taaggataac atgaaatatt ttaagaggtg
    11101 tgataatgtt aacgggtaat gaatttgtaa agcagtccct ggccctgcac ttattttttg
    11161 caaggattat gaaagagcat tccttctttc tggaagtagg attcaccccc aaggattata
    11221 attttaccaa tcaagcagac tcctttcgaa aggcttttga tgccttactg gcggatgctg
    11281 tagcgctttc gaatggtgtg gtaaatcaag aggtccttca atccggtgag gtaataacac
    11341 cttatacact aaaggcggaa acggtatcgg catattttac aggagttcgg atacagaccg
    11401 agcttactca atcggaagca agcttggccg gagacggtat gtcaaacagt tccatgcttg
    11461 aacaaagagt gtattatctg aatgataatg caataaagtt aataaattcg ttagcgcagt
    11521 ttaaaaccac cattttatca aatgtgattt catgccgcat gtttaccgta aactatccgc
    11581 tgctcattga ccatatttta agagaagcaa aattctatct gatgctggta caaaaattac
    11641 agaaccggga agacttcaat ctggaaaaag aagtatatga gcaagagacc ttctggaaca
    11701 ggattatggc ggagcattct aaatttattc gcgggctcct tgatcccaca gaaaatgaat
    11761 tgatgatcac ggctaataat ttcgggaatg aatttgatca gctgacaaat caggcaaaac
    11821 aggcaatgga taaaacaata ccgctaaaga aagttaccac cgatagtttg agcgctacca
    11881 tgaaaatccg cgatttcaaa acggcaggta ccgaaggaat tcttaactgc aaaattaaat
    11941 ccattatcat tccattgtta ggcgaccatg tattacgtga agcaaatcac tatctgcgtt
    12001 tattgaatat gttcgaaaaa aggatttaga taatacgctg gattttggcc tgcggtatcg
    12061 catcatgaac atccgcaatt tttccggggc attattgacg gtaaaatttg attttttcga
    12121 taatcttgcc gttttgggcc aaaatggccg gagttccatc cggaatccat ttcatctgct
    12181 ccgtttttct aaagttgaaa gggaaattct ggattaacag ctaaagtttc caaaggataa
    12241 gaattttagg cagggaaata atatgaatgc gcaggaatta tagcaacagg gagatgaaaa
    12301 acctatggat agattcgcat taatacttgt aattatcggc gccatcgtat ggggcgtggt
    12361 tggtatattt ggtatgaatc ctgttgcatg gatcatgcac ggttccatga ctgtactcag
    12421 cagaataatc tatactctca ttggtcttgg aggcctatgg tgtatcaagt tattgttccg
    12481 tgagcgggaa ctggtacagc accacgaata acagcccaat tagcagagcg gcaatgcaaa
    12541 aagttacgca tgccgctctg cttgttttta tcttcctttt tccacgacaa ggtcggagaa
    12601 tttaccctgg tacagaattt ggagggaatc tttcggaggc ccttcaagca attttccgct
    12661 cgatgctgaa aatcttccgc cgtgagcagg acagtcccat gtcttttccg ccgcattgaa
    12721 attcaattcg gtcgtcatat gcgtgcagga aatgtcaaga acggtgacgt tgtcgtcaaa
    12781 gtcgcggtaa atgcccgctt tttgcccgtc aaaattaatg acccggcctt cgtacggttt
    12841 cagattgcga acactttccg ttctctccag cttggatttg atgagttcgc ccacagagcc
    12901 catgacctcg gaaactgttt ttccggcaga gcttgaatag tccccgcgct ttctggaata
    12961 gagctcctca tagcggcagt ttccgctcgt gatcaaatct gcaatcatat tccccgcaag
    13021 cgtgccgttc gaaagacccc atttgccaaa acccgtggcg atataaagat tggaataatc
    13081 ggacattctg ccgatataag ggatctgatc cggtgtgtca taatcctgcg cagaccactt
    13141 tgccagaaga tcgtttgtcc ctgcaatgtg ttttgcaaat tgtatcagat tattatagtg
    13201 cagttccgta ttgtcttctc cgcgggcggt ggcatgattt tctccgacga caatcaagat
    13261 gcgtttcccg ttttccacat gtgtccggat cgaacgggtg ggatccccgg tattgatata
    13321 gctgccgtcc ggccagtctt tttccgggct tacagcaata ccgtaggcgc gttttgcgta
    13381 gagccgtgta tagaatatat tcggcccgtc gtaaattgga tactgagttg ccatgacaac
    13441 gtgctttgct tttatagtaa tatcgttttc gcaataaacg gtcttaatat catcatcgtc
    13501 taccttgatc gccttggtat cgcagtagat tcggacgcct tttgacgccg cggcttttgc
    13561 cagcgcgttt acataccgaa cggaatgaaa aatggcctga tttttaaaac cgaccatgcc
    13621 gaaattatcc ggcggaaaat ttgacttttc atagaattcc gcattgatgc caatttcttt
    13681 ggcaacttca tattctctca caaccatgtc cctatcctct tgagaagagg cataaatata
    13741 ggaggtactg tttgtaagct gacagtcgat ggattctttt tttacctgtt ccctgacaaa
    13801 atccacagcg cttgtctggg actccgcata gagttttgca tcatctatcc catgcttttc
    13861 taaaatgtgg ctgtaaataa tgtcgtgctg aatggtgatc ttgcctgttg tgttgccggt
    13921 ggtcccatcg cacaatccgc cggcttcgat gacaaccggc tttgcgcctt tctgtgccag
    13981 acagtaagcg caggtgatac cggttatccc gcttccgata attaaaatat cggtctcaag
    14041 gctttcgcta agccggggat attccgcaat ttccgcggtt gtgttccaat aggataatgt
    14101 tctttcactc aatttaatgc cctcctgata tcggttgacg ttatgatgtg caaaatatta
    14161 ttgcggatta cgtgacaggt gctaaaatac ggtgaattca tcgacattat ttacagccac
    14221 catattttaa gagaagcaaa attctatctg atgctggtac aaaaattaca gaaccgggaa
    14281 gacttcaatc tggaaaaaag aagtatatga gcaagagacc ttctggaaca ggattatggc
    14341 ggagcattct aaatttattc gcgggctcct tgatcccaca g
//
LOCUS       DATALY010000115         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_802447,
            whole genome shotgun sequence.
ACCESSION   DATALY010000115 DATALY010000000
VERSION     DATALY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802447"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="VHR42_03410"
     CDS             complement(<1..102)
                     /locus_tag="VHR42_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026260.1"
                     /translation="MKQSISVLGSTGSIGTQTLSVARHLNLHVCALAA"
     gene            complement(145..969)
                     /locus_tag="VHR42_03415"
     CDS             complement(145..969)
                     /locus_tag="VHR42_03415"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3026261.1"
                     /translation="MRTRVTSGTALVIFLAAIVLFNSSFPLALNMAIALVSVLADYEI
                     ISALGVAKNYALLIPSLLFSAVLPFITDPLLCQAAYFLYTLVIFSSLIFYHSVMTFRE
                     IGVIYSMSLMIPTALATIIGLRSVGGTHGMFYVIIGIFSAWVADVGAYFAGSFLGKHK
                     LCPNISPKKTVEGAIGGLILNVAAMLVFGYIFQVVYYARSVTVSYVSLLIIGIVGTGI
                     SILGDLSFSLIKRSCHIKDFSEIIPGHGGILDRFDSVIFEAPFVYFLVQILPIVVK"
     gene            complement(970..1677)
                     /locus_tag="VHR42_03420"
     CDS             complement(970..1677)
                     /locus_tag="VHR42_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX3026262.1"
                     /translation="MTNSVLPKHLGIIMDGNGRWAKKRGLPRSAGHAAGAAVFKDIVR
                     YCSKIGIPYLTIYAFSTENWKRPHDEVLTLVKLFKQYLDDALTNFLNENIRVRFLGDT
                     DAFDPQLQDLIARTTEVSSTHTGMVLNIALNYGGRAELCRAFRLLAKDVQAGVLSPNE
                     IDEATVSSRLYTAGQPDVDLIIRPSGEERLSNFLLWQAAYSEFLSLDVLWPDFRSADL
                     DRALEIYAHRNRRFGGI"
     gene            complement(1684..2238)
                     /gene="frr"
                     /locus_tag="VHR42_03425"
     CDS             complement(1684..2238)
                     /gene="frr"
                     /locus_tag="VHR42_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX3026263.1"
                     /translation="MNEILTDAEKRMKKSIAVLSSDYAAIRAGRANPAVLDKIRVDYY
                     GTPTALNQLAAISVSEARILTIQPWDMSVCRAIEKAIETSDLGINPQSDGKVIRLTFP
                     QLSEERRRDLAKEISKMSEECKVAIRSIRRDAMEKLKAMKKNSEMSEDELKQTEKKAQ
                     DLTDKYCKEVDSVFEEKKKEIMEI"
     gene            complement(2235..2951)
                     /gene="pyrH"
                     /locus_tag="VHR42_03430"
     CDS             complement(2235..2951)
                     /gene="pyrH"
                     /locus_tag="VHR42_03430"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX3026264.1"
                     /translation="MQPKYKRILLKLSGESLAGSHTHGIDFDTVLKICEPIRECTRLG
                     IEMGIVVGGGNFWRGRSSGKMDRTRADHMGMLATTINALGVADALEQLGVSVRVQTAI
                     EMRQIAEPYIRNRAVRHLEKGRVVVFGSGTGNPFFSTDTAAALRAAEIDADIIFKATM
                     VDGVYDCDPKKNPDAVKYDTLTFMDVLNKNLQVMDSTAATLCKDNHIPILVFNIDRPQ
                     NILSAACGESLGTIVKEEES"
BASE COUNT          752 a          805 c          763 g          746 t
ORIGIN      
        1 ggctgccagc gcacagacgt gaagattgag gtgacgcgcg acggaaaggg tttgggtccc
       61 gatggaacca gttgacccga ggactgagat gctttgtttc attagtattc tccaaacagg
      121 tgctttattg catccactta cagtttattt cacaacgatg ggaagaatct gaaccagaaa
      181 atagacaaac ggagcttcaa aaatcacgct gtcgaagcgg tccagaattc cgccgtggcc
      241 gggaattatt tcactgaaat ccttgatgtg acagctccgt ttaatcagag aaaaactcag
      301 atctccaagt atggagatac cggttccgac gatcccgatg atcagaaggg aaacatagga
      361 aacggtaacg ctgcgcgcat aataaacaac ctgaaagata tatccgaaaa ccagcatggc
      421 tgcaacgttc agaatcagcc cgccaatcgc accctccacc gtctttttgg ggctgatgtt
      481 cgggcaaagc ttatgcttgc caagaaaact gccggcaaaa taagccccta cgtcggccac
      541 ccaggcggag aaaatcccga tgatcacata aaacatgccg tgtgttccac caacgctcct
      601 cagaccgata attgttgcga gagcggttgg aatcatcagc gacatgctgt atatgacgcc
      661 aatttcccgg aaggtcatta cggaatggta aaaaatcagg gagctgaaaa tcaccagcgt
      721 atacagaaaa taggccgcct gacagagaag ggggtccgta ataaacggca gaacggcgga
      781 aaacagcagg ctgggaatca ggagggcata attctttgca acgccgagcg cggaaataat
      841 ttcgtaatcg gccagaacgg acacgagtgc aatcgccata ttcagcgcga ggggaaatga
      901 actgttaaat agcacgatgg cagcaagaaa aataacgagg gcagttcccg acgtgacccg
      961 tgttctcaat taaatccccc caaagcgtct gttgcggtga gcatagattt ccagtgcccg
     1021 gtcgaggtcc gccgaccgga aatccggcca caaaacatcc agagagagaa attccgaata
     1081 ggcggcctgc cacagaagaa aattggagag ccgctcttcc ccgcttggcc ggataatcag
     1141 gtctacatcc ggctgtccgg cagtatatag acgggaagaa acggttgctt cgtcgatttc
     1201 attcggtgaa agaactccgg cctgcacatc tttggccaaa aggcggaacg cgcggcacag
     1261 ctccgcgcgt cctccgtaat taagcgctat attcagcacc atgccggtat gtgttgaaga
     1321 aacctcagtt gtccgggcga tcaggtcctg aagctggggg tcaaaggcat cggtgtcacc
     1381 cagaaaacgg acccgaatgt tttcgttcaa aaaattggtc agcgcgtcgt cgaggtattg
     1441 tttgaatagc ttgacgaggg tgagaacctc gtcgtgcggg cgcttccagt tttcggtgga
     1501 aaaagcgtat atggtcagat aaggaattcc gattttgctg caatagcgga cgatgtcctt
     1561 gaatacggcg gctccggccg cgtgacccgc ggaccgcggc agtccccgct tttttgccca
     1621 ccggccgttg ccgtccatga tgatcccgag gtgtttgggc aaaacgctgt tcgtcatatc
     1681 ggattatatc tccatgattt cttttttctt ttcttcaaaa acgctgtcga cttctttgca
     1741 atacttatcc gtaagatcct gcgccttttt ttccgtctgc ttgagctcgt cttccgacat
     1801 ttcggagttc tttttcatgg ccttcagctt ttccatagca tcgcgccgaa tggaacggat
     1861 cgcaaccttg cattcttctg acatcttgct gatttctttt gccagatcgc ggcgccgttc
     1921 ctcagaaagc tgcgggaagg tcagacggat cacttttccg tcgctttgcg ggttgatgcc
     1981 gaggtcggag gtttcgatcg ccttttcaat tgctcggcaa acagacatat cccacggctg
     2041 aatcgtcaga attctggctt cgctgaccga gatggcggcc agctgattca gagcggttgg
     2101 ggtaccgtaa tagtccacgc ggattttatc cagaacggcc ggattggccc tgcccgcgcg
     2161 aatcgccgcg taatcggatg aaagaactgc aatgcttttt ttcatccgtt tttcggcatc
     2221 cgttaaaatt tcattcatga ctcttcctcc ttaacaattg ttccgagaga ttctccgcag
     2281 gctgcgctca aaatgttctg cgggcgatca atattgaaaa caaggatcgg gatatgattg
     2341 tccttgcaaa gcgttgccgc agtgctgtcc ataacctgaa gatttttgtt cagaacgtcc
     2401 ataaaagtca atgtgtcgta ttttaccgcg tccgggttct tcttcgggtc gcagtcgtaa
     2461 acgccgtcca ccatagtggc cttaaagatt atgtctgcgt cgatttccgc ggcgcgcaaa
     2521 gcggcggcgg tatccgttga aaaaaacgga tttccggtcc cacttccgaa caccacgact
     2581 cttccctttt ccagatgacg cactgcccgg ttccggatat acggttccgc aatctgccgc
     2641 atctcaatcg ccgtctgcac ccggacggaa acgcccagct gctccagcgc gtccgcaaca
     2701 cccaacgcat tgatggtcgt tgcgagcatt cccatgtgat cggcgcgggt gcggtccatt
     2761 ttcccgctgc tccggcctct ccagaaattc cctccgccga ccacaatgcc catctcgatt
     2821 ccaagacgcg tgcattcccg gatcggctcg catattttca gcaccgtatc aaaatcgatg
     2881 ccgtgcgtat ggctgccggc gaggctttcc ccactcagct ttaacaaaat tcgtttgtat
     2941 tttggctgca acttcaacac tcctgttcaa ttgaatctat tttacatgaa ggaccgttcc
     3001 ttgtaaagca tttccgcgcc agataagggt attattgaca tttttcacaa tttggaaaca
     3061 aattta
//
LOCUS       DATALY010000116         6903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_287327,
            whole genome shotgun sequence.
ACCESSION   DATALY010000116 DATALY010000000
VERSION     DATALY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287327"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHR42_03435"
     CDS             <1..331
                     /locus_tag="VHR42_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026265.1"
                     /translation="AFGDVFPTCLPKTKFAGVDSYLPDDIANSLRQGIVEMDQWMHGF
                     AYPDAILTGAETRSTSPVRIKRGDSFEAVGIKGLYPCGEGAGYAGGIISAAVDGMLCA
                     EKILGGR"
     gene            921..2813
                     /locus_tag="VHR42_03440"
     CDS             921..2813
                     /locus_tag="VHR42_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026266.1"
                     /translation="MGIFLAVIALFVLSLLLSTVFRKKIEETLPVSMFLVIILIYAAG
                     LTGHLNVGVYLVSILAVAAVVCLIVKLIRSRENLLRSIRTPGLAIFLLLFVFSLFLQH
                     GRVLVGWDEMSHWGLAAKNMFLLNNFGTGPDSNLIFKDYPPAAAVFEYFCLKLNGGYS
                     DSIVMMSYNLLAFSPLLIVFREVEWKQWKQIIIRLLVILALPLAFYCDYRISVADFYT
                     TIYVDPLLGILFGYMLYSWFSDKKDGFYTLKLCCTLFVMTLLKASGFGLALILLIIAA
                     LYQAMLYIRRRANHAVPAVAKKQVLQFIFMAVSVFAAEYSWKLVMASNHITKKFQTGN
                     ITLLTVINVLSGKGEPYTREVIRRFLTALFTKPLIHIGMVLPVGTVLLTAAMAGLMIL
                     LVRTEKDQTVKYRLKVTGAALIVFEFLYALSLLILYLFSFSQGEALALASFERYMNTY
                     FLGISIFLAFRLVQAKHSAVNRKRAMIALAVGIMLMTNPISFLKDSVAALAGNRHSVQ
                     KMAPYCHMALKLEQYARQDGIAKPGIYFLDSSSAEYDYLAAKYYVTPESINAFGTWSE
                     DSLRIQDLSSRFTPEYQYVYLFSVNDSFRNNFSQMFQGGKSSIQSGALYRVSRKNNMV
                     KLIACK"
     gene            2894..3949
                     /locus_tag="VHR42_03445"
     CDS             2894..3949
                     /locus_tag="VHR42_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HEX3026267.1"
                     /translation="MIVLIPAYEPDDRLTELISHLMENCGYDIIVVDDGSGQDYFSVF
                     DSVRAMGCTVLTHPVNRGKGRALKTGFEYILHSDEKTGVVTADADGQHSVVDIIRVAE
                     EIPLCPGKIVLGARRFVGGVPLRSIIGNTFNRMLFTAASGNKVHDTQTGLRGIPVSLL
                     PLMLKIEGERFEYEMEMLLEAGSSGYGFRQIFIDTIYLSGNKSSHFRPIRDSVRVCLP
                     FFKFCFSGLTSAVVDYTLLFFLQWLISSVLKLPGALFFGVLLARTASSIVNFTINRTL
                     VFRSRATKQKPEASAFHYYTLVVCLLFVNYLLLKVLTDALHVWLPLGKIIVEFILFFF
                     SYGIQRFVLFKKKPVAI"
     gene            4061..4888
                     /locus_tag="VHR42_03450"
     CDS             4061..4888
                     /locus_tag="VHR42_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HEX3026268.1"
                     /translation="MRKSEIVEKSITKKFKRSIWIPFLSGIKEYQLIKPQDKIAVCIS
                     GGKDSMLMAKCMQHLQKYSDFPFEVEYIVMDPGYNPDNRRRIEENAELLEIPIKIFES
                     GIFEYVSKVEKSPCYLCARMRRGHLYKKAEELGCNKIALGHHFDDVIETTLMSILYSG
                     QVRTMMPKLHSTHYEGLELIRPMYQVKEADVLAWKRYNDLQFIQCACRMTESIAADEN
                     GGGSKRQEMKALIKKFREENPEIAFNIFRSVYNVNLETIISYRQGDETHSFLDNYDR"
     gene            4904..5242
                     /locus_tag="VHR42_03455"
     CDS             4904..5242
                     /locus_tag="VHR42_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX3026269.1"
                     /translation="MQLQIFGRRKCFDTRKAERYFKERGIKFQSVDIDRYGLSKGELS
                     SVKNAVGIDHLIDRDGKEYERLNLKYITTGQTKEELLLNNPKLFVTPIVRNGKQATVG
                     FRPEVWKSWE"
     gene            complement(5329..6687)
                     /locus_tag="VHR42_03460"
     CDS             complement(5329..6687)
                     /locus_tag="VHR42_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3026270.1"
                     /translation="MLKDALPKINMPLPGPKAKAIIERRSKAVPNAISCGYPCVISRG
                     EGAMIEDVDGNIFLDWIGGVGVLNIGYSHPELVSAVKDQADHFFHAMMNIVTHEGYIK
                     LAEKMNEIVPVKGNNKKTMFVNSGAEADENAVKIAKAFTGRPNIIVFSGAFHGRTLLT
                     MSMTSKKAYAVGMGPFPDGVYRAEFPYLYRAPKGYTEQEAIDYYLEKLNQVFEEASPA
                     EYVAAIVVEPIQGEGGFIPAPMEWVKAVRKLCDEKGILLIADEVQTGFARSGKMFVSN
                     YWAEAGCAPDIMTAAKSIAGGVPLSAVTARAEIFDSVKGGVIGGTYGGNALACASALK
                     VIEVMERDHLCDRSIEISKKVSTVFHTWKDSYEVVGDVRGIGCMMGIEFVTDKSSKKP
                     NSKLTAAIIREAALNGLLVEGAGIYGNVIRFLCPLVVTDAQIDSGLSILEAAIKKCMA
                     AQ"
     gene            complement(6739..>6903)
                     /locus_tag="VHR42_03465"
     CDS             complement(6739..>6903)
                     /locus_tag="VHR42_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX3026271.1"
                     /translation="AKINLTTTLGEQGVKEEDIDWMTENCMKVSAAGIANHPVVFSKE
                     AIRRIYRKAL"
BASE COUNT         1716 a         1519 c         1784 g         1884 t
ORIGIN      
        1 agcttttggg gatgtttttc cgacctgttt gccaaagaca aagttcgccg gggtggattc
       61 ctatttgccg gacgatatcg cgaattcatt gcggcagggt atcgtggaaa tggatcagtg
      121 gatgcacggc ttcgcttatc cggacgcgat cctgaccggc gccgaaacac gcagcacctc
      181 accggttcgg atcaaaaggg gtgacagctt tgaggcggtc gggatcaaag ggctttaccc
      241 ttgcggcgag ggagcaggat atgccggggg aatcatttcg gcagcggtgg acggaatgct
      301 gtgcgccgaa aagatcctcg gcggcaggta atgggttgag ctgcgaagca tccgacaagt
      361 tatgaacaaa aaagcgtacc ctggaacgca tcaaaaatac gggaaagcaa aaggatgtga
      421 atgaaatgtc ttttaaaaat ccggacaggt taaaaaagaa gaagccgatt gtaaaaaaag
      481 ggacttcaaa gtcaaaaacc gacagcaaaa caataccgcc taaaaagcca aaatagagaa
      541 atgctgtgat gtcactgaaa attgccaaaa caaccaagaa tttatgcgat tatagcggat
      601 ctgtttttgc cggctgggat atcatgtccc ggccggttat tgttttagat cggtgcaact
      661 aaaggtgatg gaatgattcc gttccttatg agcgcaggaa agcatcgcct gttcaggcag
      721 gaaaaccgga atcaaaggaa ttttagaggc gtcgcccaaa aaacggaacc gataaatcat
      781 ggattgacag ttgaagtaga gcgggatcgg atgtataatt atttctgtga gtcataaaaa
      841 gaaaaacgga actgttagta acatgttgct gacttctgta tggcggtctc ataattgaca
      901 gcaaagggtt tgtatttgac atgggaattt ttcttgcggt tatcgcgctg tttgtacttt
      961 ctctgctatt gtcgacagtc tttcgcaaaa agattgagga aactctgccg gtttcaatgt
     1021 ttttagtcat tatcctgatc tatgccgcag ggctgacggg ccatctgaat gtgggcgttt
     1081 atcttgtatc cattcttgcc gtggccgctg ttgtctgttt gattgtgaaa ttgatccggt
     1141 cgcgcgagaa tttgcttcgc agcatacgaa cccccgggct tgcaattttt ctgctgcttt
     1201 ttgttttcag cctttttctc cagcacggca gagtgctggt tgggtgggac gaaatgtcac
     1261 actggggcct tgcagccaaa aacatgtttc tgttaaacaa tttcgggacg ggcccggatt
     1321 cgaacctgat ctttaaagac tatcctcctg cggcggctgt ttttgaatac ttttgcctga
     1381 agctgaacgg cggttacagc gacagtattg tcatgatgag ctataatctt cttgcctttt
     1441 caccgctgct gattgttttc cgggaagtgg agtggaaaca atggaagcaa attatcattc
     1501 ggcttctggt gattttggcg cttccgcttg cattttattg tgattatcgc atttcggtcg
     1561 ctgattttta taccaccatt tatgttgacc cgcttctcgg aatcctgttc ggatatatgc
     1621 tgtactcatg gttttcagat aagaaagacg gcttttatac cctgaaactg tgctgtacgc
     1681 tttttgtgat gactctgcta aaggcttccg gttttggcct tgcgctgatt ctattaatca
     1741 tagcggccct gtaccaggcg atgttatata tcaggcgccg tgcgaatcac gcggtgccgg
     1801 ctgtcgccaa aaagcaagtg ctccaattta tttttatggc ggtttccgtt tttgcggccg
     1861 aatattcgtg gaaactggta atggcctcca accatattac aaagaaattc cagacgggaa
     1921 acattactct tcttactgtg attaacgtgc tttccggaaa aggagaaccc tatacgcggg
     1981 aagtgatccg gcgctttttg accgccctgt tcacaaagcc tctgattcat atcggaatgg
     2041 tcctgccggt ggggacggta ttgctgactg cggcaatggc cggactgatg attcttcttg
     2101 tgcgcaccga aaaagatcag acggtaaaat accgtctgaa agttacggga gctgctttaa
     2161 tcgtttttga atttctttat gcgttgagtt tgctgattct ttatttgttt tcattctctc
     2221 agggtgaggc gctggctctg gcttcattcg agcgttatat gaatacttac tttttgggca
     2281 tatcgatttt tctcgctttc cggcttgttc aggcaaaaca cagcgcggtg aatcgaaaac
     2341 gggcaatgat tgcgctggct gtcggtatca tgctgatgac aaacccgatt tcttttctaa
     2401 aagattcagt cgccgctctg gcgggaaacc ggcattccgt acaaaaaatg gcgccgtatt
     2461 gtcatatggc tctgaaactg gagcagtacg cgcgtcagga tggcattgcc aaacccggaa
     2521 tttattttct ggacagttca tcggctgaat atgattatct ggcggccaaa tattacgtta
     2581 cgccggaatc aattaacgcc tttggaacct ggtcggagga ttcgctcaga attcaggatc
     2641 tcagcagccg gtttaccccg gaatatcagt atgtttatct gttttcggtc aatgattctt
     2701 ttcgaaataa cttttcgcaa atgtttcagg gtggtaaatc gtccattcag tcgggtgcgc
     2761 tttaccgtgt atcaaggaaa aacaacatgg tgaagcttat agcctgcaaa taagaaacgg
     2821 tgaacaaatc accggaaatt tatgaacgcc ggcaaataca agtcagaatt tttaaaaact
     2881 tggagtgaag taaatgatcg tcctgatacc cgcgtacgaa ccggatgacc ggttgacgga
     2941 actgatctcc catctgatgg aaaattgcgg ctacgatatt attgtcgtgg acgatggcag
     3001 cggacaagac tatttctccg tgtttgattc agtacgtgct atgggttgca ctgtcctcac
     3061 ccatccggtg aacaggggaa agggaagggc gctgaaaaca ggatttgaat atatccttca
     3121 ttccgatgaa aaaacaggag tggtgacagc ggatgcggac ggtcagcatt cggtggttga
     3181 tatcatacgg gtggcagaag aaataccttt gtgcccgggt aaaattgtgc tgggagcgcg
     3241 gcggtttgta ggcggcgttc ctctgcgcag tatcattgga aacacgttta accgcatgct
     3301 cttcacggcc gcaagcggaa acaaggtaca cgatactcag acagggcttc gcggcatacc
     3361 ggtatcgctt ctgcctctga tgcttaaaat tgaaggcgaa cgttttgaat atgagatgga
     3421 aatgctgctt gaggcgggtt cttccggcta tgggttccgt cagatattta ttgataccat
     3481 ttatctttcg ggcaacaaat cgtctcattt tcggccaatc cgcgattcgg tgcgtgtgtg
     3541 tcttccgttt tttaaattct gtttttccgg cttgacgtcg gccgttgttg attataccct
     3601 gctgtttttt ttgcagtggc tgatcagttc ggttctcaaa cttccgggcg cgctgttttt
     3661 cggcgttctg ctggcgcgta ccgccagctc catcgtcaac ttcaccatca accggacgct
     3721 tgtatttcgc tccagagcga ccaaacagaa accggaagca agcgcgttcc actattatac
     3781 gcttgttgtt tgtctgcttt tcgtgaatta tctgcttctg aaagtgctga cagacgctct
     3841 gcatgtctgg ctgccgctgg gcaagattat tgtggaattc attcttttct ttttcagtta
     3901 cggtatccag aggtttgttc ttttcaagaa aaagccggta gcaatttaat cggaaaggtt
     3961 catcaatccg ttctgtcacg aggcatatgc aatgtatttc tgtcgatcgg ccgaaaaaga
     4021 tcgggagcga gtcaatatca accggtaagg tagggcttat atgcgaaaat ccgagattgt
     4081 ggaaaagagc attacaaaaa aatttaaaag gtcgatttgg attccatttt tatccggtat
     4141 caaggaatac cagctgatta agccgcagga taaaattgcg gtttgtattt ccggcggaaa
     4201 agattcgatg ctgatggcaa aatgtatgca gcatcttcaa aaatacagtg actttccgtt
     4261 tgaagtggaa tatatcgtta tggatccggg ttacaatccg gataaccggc ggcggatcga
     4321 agaaaatgcg gaacttcttg aaattccgat taaaattttt gaatccggaa tttttgaata
     4381 tgtatctaag gttgaaaaat cgccctgcta tctgtgcgcg agaatgcgcc gggggcatct
     4441 ctataaaaaa gcggaggaac tgggctgcaa taaaattgcg cttggacatc attttgatga
     4501 tgtgattgaa accaccctga tgagcatact atacagcggt caggtgagga cgatgatgcc
     4561 taagctccac agcactcatt atgaggggct ggagctgatc cgcccgatgt atcaggtaaa
     4621 agaagccgat gtgttggcct ggaagcggta taacgacctg cagttcattc aatgcgcctg
     4681 tcggatgacg gaaagcatag ccgccgatga gaacggcggg ggttccaaac ggcaggagat
     4741 gaaagcgtta ataaagaaat tcagggagga gaatcctgaa attgctttca atatcttccg
     4801 cagcgtttac aatgtaaatc tggagaccat catcagctac cgccaggggg acgaaaccca
     4861 tagctttttg gacaattatg accgatagaa tgggggagag actttgcagt tacagatttt
     4921 cgggcgaagg aaatgcttcg atacccgtaa agcggaacga tatttcaagg aacgcggaat
     4981 taagtttcaa agtgtcgata tcgatcgcta cggattgagc aaaggcgaac tttccagcgt
     5041 aaagaatgcg gtcgggattg atcacctgat tgaccgggac ggcaaggaat atgagcgcct
     5101 taatttaaaa tatatcacaa ccggccaaac caaagaggaa ttgctgctga ataacccaaa
     5161 actgtttgtt acgccgattg ttcgaaacgg gaaacaagca acggtgggtt tccggccgga
     5221 ggtttggaag agctgggagt aagcagaaaa aggtttcaga gaacgaaaaa ggccgcgcaa
     5281 acagcgcggc ctttcctatt gcaggacttt tttagatggc tttaccggtt actgtgctgc
     5341 catgcatttt ttgatcgcgg cttccagtat ggagaggccg ctgtcgatct gggcgtccgt
     5401 tacgaccagc gggcacagga agcggattac gttgccgtaa atgcccgcac cttcgaccag
     5461 aagaccgttt aaagctgcct ccctgatgat ggcggcggtc agtttgctgt tgggcttttt
     5521 gcttgatttg tccgtgacaa attcgatgcc catcatgcag ccgatgcccc ggacgtcacc
     5581 gacaacttca tagctgtctt tccaggtatg aaagacagtg ctgacctttt tcgatatttc
     5641 gatggagcgg tcgcagagat gatcgcgttc catcacttcg ataactttta aagcagacgc
     5701 gcaggcaaga gcatttccgc cgtaggtgcc gccgatgacg ccgcctttga ccgaatcgaa
     5761 tatttcagct ctggcggtga cggcgctcag cggtacgccg cccgcaatgg atttcgcggc
     5821 ggtcatgata tcgggagcgc agcctgcctc ggcccagtag ttggagacaa acattttacc
     5881 cgaacgggca aaaccggtct ggacctcgtc ggcaatcagc aaaatacctt tttcatcgca
     5941 gagcttgcgg acagccttga cccattccat cggagcgggg ataaagccgc cttcgccctg
     6001 aatcggctcc acgacaatcg cggccacata ttcggcaggg gaggcctctt caaacacctg
     6061 gttcagtttt tcgagatagt agtcaatcgc ttcctgctcg gtataacctt tgggcgcgcg
     6121 ataaagatag gggaattccg cgcggtagac cccgtcgggg aacgggccca ttccaaccgc
     6181 gtacgccttt ttggaggtca tggacatagt cagcagggtt cttccgtgaa atgctcccga
     6241 aaaaacgata atgttcgggc ggccggtgaa agcttttgca atttttaccg cattttcatc
     6301 ggcttcggcg ccggagttta caaacatggt ctttttatta ttgcctttga ccggaacaat
     6361 ctcgttcatt ttttcagcca gtttgatata gccctcgtgg gtaacaatat tcatcatggc
     6421 gtgaaaaaag tgatcggcct ggtcttttac ggcgcttaca agttccggat ggctatagcc
     6481 gatgttgagt acgcctactc cgccgatcca atcaagaaag atgtttccgt cgacgtcttc
     6541 aatcatggcg ccttcgcctc tgctgatgac gcagggatag ccgcagctga tggcgttcgg
     6601 aacggctttg gaacgtcttt cgataatcgc tttggccttc gggccgggca gcggcatgtt
     6661 gattttcggt aaggcatctt ttaacatgat aaaactcctt taaatgataa aattgttcat
     6721 ggtgtctgtt tgaatacttt acagtgcttt acggtaaatt cgccggatgg cttctttgct
     6781 gaaaacgaca ggatggttgg cgatgcccgc ggctgaaacc ttcatgcagt tttcggtcat
     6841 ccagtcaata tcctcttctt tgacaccctg ctcgccaagc gtggttgtca ggttgatttt
     6901 cgc
//
LOCUS       DATALY010000117         8536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_802684,
            whole genome shotgun sequence.
ACCESSION   DATALY010000117 DATALY010000000
VERSION     DATALY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802684"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VHR42_03470"
     CDS             <1..351
                     /locus_tag="VHR42_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HEX3026272.1"
                     /translation="NLMEMQWIHSPTVNSGEFLHGPFETTDKITPYFVLVSEGRTRPV
                     DERALNFLRRYAERIHVLDAKELGINRINDTVAEFFNHLIFSSVLNNVYLRELSYSKD
                     HPYTNRRYMWKTQY"
     gene            407..1252
                     /locus_tag="VHR42_03475"
     CDS             407..1252
                     /locus_tag="VHR42_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3026273.1"
                     /translation="MAKIIGIGLNVVDKYESLGIMYPGGNEMNVAVYARWIGADAAFI
                     GVFGNDRAAIHNTNVLKEVGVDLSRCRYVEGENGYATVQLVNGDRVFLSTNNGGVTGL
                     NPIVLNNEDLQYIKNFDVVSASINARLFLPEIKKLSGSSIPVSYDFSDLYNEEIIRET
                     APHATFSFLSCGNMSLEKASELLKKVYRLGSRYVIGTMGPRGSLLYFGKEVRQDPIDV
                     EIVDTMGAGDSFIAAFLNAFYSYKEKGVEPGEKELQNCMLEATKYATKICTIQGAIGY
                     ETSIW"
     gene            1288..2589
                     /locus_tag="VHR42_03480"
     CDS             1288..2589
                     /locus_tag="VHR42_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3026274.1"
                     /translation="MRIKRRFLSVASVFLCLILILSGCGGTKSGSSTSSTKPVTITFL
                     HKWPEDSNMAFFKQVIKSFEAKHPNVTIKMEAVGDQDIKDKLRVMVGGTCPDIFFSWS
                     GEFANKFVRAGKVLDFTPYFTKDSDWQNSFLQAALKRGSFNDKYYGIPMRLDLQFFIY
                     NKKIFQNYGLSVPKTWNEFLNVCKTLKQHNVTPILFGNQQAWAGAHYITALNSMYVPA
                     SVKSKDDAPATGSFTNENYLKALNTFKNLETSGYMNTNVNSTTFYQVREIFYTGKGGM
                     FLDVLSDFSKYEQNMGKGNWGYFRFPIDNTGSGDQNFIVGGADMFMVSKQCKNPDVAV
                     EFLKFMTNKENAANFAKTTGYPIPVKGAVNKQNSLSEVVDAMNYISNVDTIEEWLDTS
                     LESRVADKYLTSLQDIFNNKSTSDALKEIQSTAKTVQSDYK"
     gene            2729..3610
                     /locus_tag="VHR42_03485"
     CDS             2729..3610
                     /locus_tag="VHR42_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015240527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3026275.1"
                     /translation="MWRNKYRIFFFYLSPAIIFIFLFIYYPVFINFYYSLFSWSSFSP
                     SMKFIGFTNYIRMFQDQVIAISVKNNFIFAVISVIFQVGLSMVLASVLEERFLRKFSS
                     FFRTVYFLPSLISITVCGIMFQLLFNPNMGIINAALTALGFNVSHLDLLGNAGTAIYT
                     IVGVSQWQYIGYNTLLFIVAIQKIPAELYEASVIDGAGIIKKFRFITVPQIREMTLVT
                     MVVTMIGAFKVFDEVYIMTGGGPGNSTQVLATYLQKSGFNNDEMGYASTIAVLVFVIT
                     FALSIIQMKISKVGTEQ"
     gene            3623..4459
                     /locus_tag="VHR42_03490"
     CDS             3623..4459
                     /locus_tag="VHR42_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010281145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3026276.1"
                     /translation="MEDKSFKARLIRILIFLVLIIFAVLELYPFLWMIIGSFKNNAEI
                     FVGSWHIPRVWRWDNFQHAWSSGVSSYLFNSLFVTVVATALSTIISAFAAYPLARVKM
                     KLNRVILMMILSGLMLAPQVALIPLYKALQTLAIYNTYWAMILPDTAFRIPFTTFLIW
                     SYFLTIPKELEEAAFADGAGTQKIFWKIILPVSKPILATSILLSARVIWNEFLFALIF
                     VEDNSLKTITVGLKGMISNTETNWGIIIAGMTLSTAPIIILFLAAQKQFIRGMAAGSV
                     KG"
     gene            4483..5325
                     /locus_tag="VHR42_03495"
     CDS             4483..5325
                     /locus_tag="VHR42_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3026277.1"
                     /translation="MAGFTLTKLALGNFHYKNYPLTYFLESACRLGIESIELWAAAPH
                     LYVYDYDKEMIGGLKKELHTRNLNLICFTPEQCVYPTNLSSREEYIRKRSVDYFIRSI
                     EVSEELGCGMMVVTPGQDYFNEDLGEAWKRCRDSLETLSREAEKRGVILVLEPLPLYM
                     THIINGVAELAKMLREVSSPYLKGMLDTDQMAVYGKDSVANYLSTLGSDLRHVHFIDG
                     RPGGHLVPGDGKLPMERYLFDLLRGGYSGYLSLEILDNNYVTHPESASKDSIRWMADH
                     IRNS"
     gene            5616..6191
                     /locus_tag="VHR42_03500"
                     /pseudo
     CDS             5616..6191
                     /locus_tag="VHR42_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            6262..6612
                     /locus_tag="VHR42_03505"
     CDS             6262..6612
                     /locus_tag="VHR42_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3026278.1"
                     /translation="MVQGDFSVTCGYHAVRTWIEAGCLPDAVFASDDNTAFGILDAAR
                     EYGIDVPGSLSIIGYDDHPYASLLHPGISTIRQPSERIGSAAAELLMDLIEGRPARTK
                     ITLKPFLILRGTTK"
     gene            complement(6656..7693)
                     /locus_tag="VHR42_03510"
     CDS             complement(6656..7693)
                     /locus_tag="VHR42_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3026279.1"
                     /translation="MAYMWQEIMQQPGILTDCLKKNEEEIQKIVKAVRQKDIRYVVIA
                     ARGTSDNAAVYGKYILEIMTGIPVALAAPSVVTIYGKKLRLDHTLVIGISQSGRAGDA
                     AEVIECANQSDAVTVSITNFPDSPLAGLSDFHLSCEAGLEKSVAATKTFTAQMILLAA
                     LAAAIADNTELREELGRVPAGIEKILQMNPEIEKVVQRYRFMNECFVLARGVNYPIAL
                     EAALKIQETSYVRAKAFAVSDFYHGPMAMIEKDMPVFIFSPDGPMLKDVRQMIAKLAE
                     NKADLLIVSNLKDVCALGQASMLLNAEGSELVFPFYNAVVAQMLAYHLSVLKQYNPDS
                     PRMLQKVTITR"
     gene            7927..>8536
                     /locus_tag="VHR42_03515"
     CDS             7927..>8536
                     /locus_tag="VHR42_03515"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3026280.1"
                     /translation="MERKLVFRLPSSWNQNFFYLINNLAGQSAVFDRLMIFFAQAMVG
                     ALAATVAAVYLTGVVTKSPSLRREAVSTGLITLFGLLFTALFGAIFPTFRPFAGHNVH
                     QLIAHPASSSFPSHHSFSSMSLSLGMLRVRKGLGLGMILISVLIGFSRVYVGVHYPAD
                     VLGGFLLAFICHIAYRKPIKKKIESAYLKLEEAVVSKLRPRSF"
BASE COUNT         2263 a         2017 c         1980 g         2276 t
ORIGIN      
        1 aatctcatgg aaatgcagtg gattcattct ccgaccgtca acagcggtga gtttcttcac
       61 ggaccttttg aaacaacgga taaaataacc ccttattttg ttttggtcag cgaaggacgt
      121 acccggcctg tcgatgagag ggcattgaat ttccttcgga gatacgccga gagaatccac
      181 gtgctcgacg ccaaagagct tggaatcaac cggattaatg atacggttgc agaatttttc
      241 aaccacctta tcttttcttc cgtcttaaat aacgtatacc tcagggaact gtcttattct
      301 aaggatcatc cctatacgaa cagaaggtat atgtggaaaa cacagtattg acggagtgag
      361 gttcaaacgg cgttcctgta aaaagtgtct gtccaaggag gattttatgg ctaaaatcat
      421 tggcatcggc ctaaacgtgg tagacaaata cgaaagcctt ggcatcatgt atcccggtgg
      481 gaatgaaatg aatgtggcgg tttatgcccg atggattggc gcagacgcgg ctttcatagg
      541 agtcttcggg aacgacaggg cggcgattca taacacgaac gttttgaaag aggttggagt
      601 ggatctatcc agatgccgct atgttgaggg ggaaaacggc tatgcaacgg ttcagctggt
      661 aaacggagac cgcgtttttc taagtacaaa taatggggga gttaccggcc tgaacccgat
      721 tgttttaaac aacgaagact tgcaatacat aaaaaatttc gacgttgttt cggccagcat
      781 caacgcaagg ctttttcttc cggaaattaa aaagctgagc ggttcttcca taccggtaag
      841 ttatgatttt tccgatttat acaacgaaga aatcatcaga gaaacagcgc cgcacgccac
      901 attcagcttt ttatcctgtg ggaatatgtc gcttgaaaaa gcttccgaat tgctgaaaaa
      961 agtatatcgc cttggaagca gatatgtgat tgggacaatg gggccaaggg gatctcttct
     1021 ctattttggg aaggaggtaa gacaggatcc tatcgacgtt gaaatagttg ataccatggg
     1081 cgccggcgat tcgttcatcg ccgcatttct aaatgcattt tattcttata aagaaaaggg
     1141 ggttgaaccg ggggaaaaag agcttcaaaa ctgtatgctt gaagctacaa agtacgcgac
     1201 aaagatatgt acgatacaag gagctatcgg ttacgaaacc agcatttggt agtctgatca
     1261 tgtgatagga aagagaggaa gaatgttatg cgtatcaaaa ggagattcct ttcagttgca
     1321 agtgtttttc tttgtttgat cctgatactt tccggctgcg gcggcacaaa gtcgggttcc
     1381 tctaccagca gtacaaaacc ggtgacgatc acgttccttc acaaatggcc ggaagacagt
     1441 aatatggcct tctttaaaca agtaatcaaa agctttgaag caaagcatcc aaatgttacg
     1501 ataaaaatgg aagccgtcgg cgaccaggat atcaaagata aactacgggt catggtcggg
     1561 ggcacctgcc cggatatttt cttctcctgg agcggcgaat ttgccaacaa gttcgtacgg
     1621 gccggcaagg ttctcgattt cacaccctat tttacgaaag attcggactg gcaaaacagt
     1681 ttccttcagg ctgccctgaa acgcggcagc tttaacgaca agtattatgg aattcccatg
     1741 agacttgacc tgcagttctt tatctacaac aaaaaaatat ttcaaaatta cggtttgagt
     1801 gtcccgaaaa catggaatga attcttaaat gtttgtaaaa cattaaagca acataacgtc
     1861 acccccatcc ttttcggaaa ccagcaggct tgggccggcg ctcattacat cacggctttg
     1921 aactccatgt atgttcccgc aagtgtgaaa tcaaaagatg acgctccagc caccggcagt
     1981 tttacaaacg aaaattatct caaggcttta aacacgttca agaacctcga aacttccggt
     2041 tatatgaata ccaatgtcaa ctctacaacg ttttatcagg tcagggaaat tttctacacc
     2101 ggcaagggcg gaatgttcct ggatgttctc agcgactttt caaagtatga gcagaacatg
     2161 ggcaaaggca attggggata tttcagattt cccattgaca ataccggatc cggcgatcaa
     2221 aacttcattg tcggcggtgc ggatatgttc atggtctcca agcaatgtaa aaaccctgat
     2281 gtcgccgttg aatttctgaa gtttatgacc aataaagaaa acgccgcaaa ttttgcaaaa
     2341 accacaggat acccgattcc tgtcaaagga gcggtaaaca aacaaaactc attatccgaa
     2401 gtggtggacg ctatgaatta catcagcaat gtggatacca ttgaagaatg gctggatacc
     2461 tcccttgaat cccgggtagc cgataaatat ctgaccagtt tgcaggatat ttttaacaac
     2521 aaatccactt ccgatgcttt gaaagaaatt caaagtacgg ctaaaacggt gcagagtgat
     2581 tacaaataag attccgggat ctgagacaaa aatccgagag ggtaaagaaa gcggtgtcgg
     2641 atccacaccg aaatagggaa cggaatacat aataatgagt tttaacgctc attattatgt
     2701 attctttata ttaaaaggtg gtaattttat gtggcgtaat aaatatcgca tattcttctt
     2761 ttatctgtcc cctgccatca tttttatttt tctgtttatt tattaccccg tgttcattaa
     2821 cttttattac agcttattca gttggagctc cttctcacca agcatgaaat ttattggatt
     2881 cacgaattac ataagaatgt ttcaagacca ggtaatcgcg atttccgtca aaaacaattt
     2941 tatttttgcc gtaatatcgg tcatttttca agttggcctt tccatggttc tggcatcggt
     3001 actcgaagaa cggtttttga gaaaattcag cagctttttt cgaactgttt attttctgcc
     3061 gtctcttatt tctatcaccg tttgcggcat catgttccag cttttattca accccaacat
     3121 gggtattatt aacgcggcat taactgcgct tggctttaac gtcagccatt tagacctttt
     3181 aggtaatgca ggcaccgcaa tttataccat tgtcggggtt tctcaatggc aatatatcgg
     3241 ttacaatacg ctgcttttta ttgttgccat tcaaaaaatc cccgccgaac tttatgaagc
     3301 ctctgtgatc gacggcgccg ggatcatcaa aaagttccgc tttattaccg ttccgcaaat
     3361 cagggagatg accctggtga caatggttgt aacaatgatc ggggcattta aagtctttga
     3421 cgaggtttac atcatgaccg gcggcggccc gggcaattcc acgcaggttt tggccaccta
     3481 tcttcaaaaa tcaggcttta acaatgacga aatgggatat gcatcgacca ttgcagtcct
     3541 ggtatttgtc atcacattcg cattgtcaat catccaaatg aaaatttcaa aagtaggaac
     3601 cgaacaatag ggagtgcaga atatggaaga caaatcattc aaagccagac tgatccgaat
     3661 tcttattttt ttggttttaa ttatttttgc ggttctagag ctgtatccgt ttctttggat
     3721 gatcatcggg agctttaaaa acaacgcaga aattttcgta ggctcctggc acataccaag
     3781 ggtctggaga tgggataatt tccagcacgc ctggagcagc ggcgtcagca gctatctgtt
     3841 taacagtctt ttcgtcaccg tcgtcgcaac cgcgctttca accatcatca gcgcatttgc
     3901 ggcctatccg ctggcaaggg taaaaatgaa gttaaacaga gtaatattaa tgatgatttt
     3961 gtccgggctt atgctcgcac cgcaagtcgc acttattccg ctctataaag ccctccagac
     4021 cctggcgatc tacaacacct attgggcaat gattttaccg gatacggctt ttcgcattcc
     4081 gtttaccacc tttctcatat ggtcttattt tttaacgatt cccaaagagc ttgaagaagc
     4141 cgcatttgcc gacggggccg gcacgcaaaa aatattctgg aaaattatcc ttccggtaag
     4201 caaaccgatt cttgcgacat ccatccttct ttccgcaaga gtgatctgga atgaattcct
     4261 gtttgctttg atctttgtgg aagataacag cttaaaaacc ataactgtcg gattaaaagg
     4321 catgatcagc aataccgaaa ccaactgggg cattattatt gccggtatga cgctgtccac
     4381 agccccgatc atcatcctgt ttcttgccgc tcaaaagcag ttcattcggg gtatggcagc
     4441 gggaagcgtc aagggataat taattggaaa ggggtaaaat caatggctgg gttcacctta
     4501 acaaagctgg cactcggcaa tttccattac aagaactatc ctctcaccta ctttttagaa
     4561 tccgcctgcc gattgggaat cgaaagcatt gagctttggg ctgccgcacc gcatttatat
     4621 gtttatgatt atgataaaga gatgatcggc ggattaaaaa aggagcttca taccagaaac
     4681 ttaaatttga tttgttttac accggaacag tgtgtctatc cgaccaacct gtcatcccgt
     4741 gaagaataca tccgtaaaag aagtgtcgat tattttattc gctcgattga agtgtcggaa
     4801 gaactggggt gcggcatgat ggttgtaacc ccagggcagg attattttaa cgaggatctg
     4861 ggtgaagcgt ggaagcgatg ccgtgactct ctggaaacat taagccggga agcggaaaaa
     4921 aggggcgtaa ttcttgtgct ggagccgctc ccactttata tgacgcatat tatcaacggc
     4981 gtggccgagc ttgcgaaaat gctcagagaa gtttcttcac cgtaccttaa aggcatgctc
     5041 gacaccgacc aaatggccgt ctatggcaaa gacagcgtcg caaattattt gagcacactg
     5101 ggaagcgacc tgagacatgt ccatttcatt gacggcaggc ccggcgggca tctggtgcct
     5161 ggagacggaa agctgcccat ggaacgttac ctttttgacc tcctgcgcgg cggatacagc
     5221 ggatacctgt ccctcgaaat tctggacaac aactatgtga cacatcctga atcggcaagt
     5281 aaggactcta tccgatggat ggcagatcat attagaaatt cataaggaac aagaccgctt
     5341 gctgtaattc agaaagccta tcgattgtcg ttgaagtcga aaagcatggt ttttaattcc
     5401 gtatccgttt ttcggaattc cgtttacaat ccgtcatctc ttttcccatt gaaaaaacgt
     5461 tccggtcact ctataataag acgagacagg aacatcatcg gccgagcgcg ggtttcgcag
     5521 aagcatgccg cccgcggcct ttattccgca cagccgcgca gccttttgca gctaacggca
     5581 ggcggaggta ccgttttttc cggaaaggag acgctatgcc agcaacgatt ttcgatattg
     5641 cgcgcagcgc gggggtttca gtcgtcaccg tttcaagggt tatcaaccat tacccatctg
     5701 tgcgggaaag caaccgtctg aaagttctgt ccgccatgaa ggaactgggt tatcagccca
     5761 acgcggccgc cagaacgctg gcgcgcggca aaaccggcat gattggaatg gtgatccccg
     5821 atcttgcgga cacttttatg gctcagatta tgtcgtcggc tgaaaagacg ctcaaggaga
     5881 aaggcctgct tctggtggtt tcctccgcgg caaaaagccc cgacctgctc gaaagcggat
     5941 gcgcccggct attcaccgag tatcgggtgg acggcatcat tattcagacg cctattttaa
     6001 acgacagcta cattcttgag cttgaaaagc gaaaaatacc atttgtttta ctggaccagt
     6061 atcagctcaa catgcaggtt cccactattg tggtggataa tttcagcgga ggctacgaag
     6121 caaccgaatg cctgattaaa agcggagcaa agaaaatcgc ccatatcacc ggaaacgcca
     6181 tttatgaaag cagccgcgaa cggacccggg gggtttctgc aagccctggg cgaccaccga
     6241 atcgaatccc ggagcgaatg gatggttcag ggagactttt ccgttacctg cggctatcat
     6301 gcggtccgga cctggatcga ggccggatgc ctgcccgacg ccgtattcgc gtcggacgac
     6361 aacaccgctt tcggtatatt ggacgcagcc cgggagtatg ggatcgacgt acccggcagc
     6421 ctgtcgatca ttggctatga cgaccatccc tatgcgtcgc tgctgcaccc cggaatcagc
     6481 acgatccgcc agccttccga acgaatcggc agcgctgccg cagagttgct gatggatctg
     6541 attgaaggcc gtccggcccg cacaaaaatt acactcaaac cttttctgat tcttcgcggc
     6601 accacaaaat aaaggctgcc ccgcagttcg taatcgtgaa ctgcggggca ttcatttacc
     6661 gggtaatcgt aaccttctgg agcattcgcg gactgtcggg attgtattgt ttcaacaccg
     6721 acagatgata agccagcatc tgcgccacga cggcgttgta gaacgggaaa accaattcgc
     6781 tgccttccgc attcagtagc atggaggcct gacccagcgc gcagacatcc tttaggttgg
     6841 aaacgatcag cagatccgct ttgttttcgg caagctttgc gatcatctgc cggacgtctt
     6901 tcagcatcgg accgtctggt gaaaaaataa agaccggcat atccttttcg atcatggcca
     6961 tcgggccgtg ataaaaatcc gacacggcaa acgccttggc ccggacatag gacgtttcct
     7021 gtattttgag cgccgcttcc agcgcgatcg ggtagttcac gccccgggcc aaaacaaagc
     7081 attcgttcat aaaccggtag cgctgcacga ccttttcgat ttccggattc atctgcagta
     7141 ttttttcaat tccggcgggg acgcgcccaa gctcttccct cagctccgtg ttgtctgcga
     7201 tggccgccgc aagcgctgcc agaagaatca tctgcgccgt aaaagtcttg gtcgcggcaa
     7261 cgcttttttc aagccccgct tcacaggaaa gatgaaaatc cgaaagcccc gccaacgggg
     7321 aatcgggaaa gttggtgatg ctgacggtta cggcgtcgga ttgatttgcg cattcgatca
     7381 cttccgccgc gtccccggcc ctgcccgact gggaaattcc tatgacaagc gtatgatcca
     7441 gcctgagttt cttcccatat atcgtaacaa ccgacggagc ggcaagcgca accggaatcc
     7501 ccgtcataat ttcaagaata tactttccgt aaacggcagc gttatccgac gttcccctcg
     7561 cggcgattac cacatatcga atatcttttt gtctaaccgc cttcacaatt ttttgtatct
     7621 cttcttcatt ttttttaaga cagtcggtaa ggattcccgg ctgctgcata atttcctgcc
     7681 acatataagc catgttaata tccatcccct ttgtttttcc gttccatttt tcttcatatt
     7741 atcatagaag ctgcccactg tcaattaaac cgctttaatt ttgttgtata tgttgcttga
     7801 attcgtacca catattttag atatgctgac aatactaagt ggaaaggcag tttggaaaac
     7861 acctgcattt tctttagttt tccgttataa taaaataata taaaaaagcc cggtcacccg
     7921 gacgaaatgg aacgaaagct ggtatttcga ttgccgtcat catggaatca gaactttttt
     7981 tatcttatca ataatctggc gggacaaagc gcagtctttg accgcctgat gatatttttc
     8041 gctcaggcta tggtcggtgc gctggccgca accgtcgccg ccgtctattt gaccggcgtg
     8101 gtgacaaaaa gcccgtcgct gaggcgtgaa gccgtttcaa cggggcttat caccttgttt
     8161 ggtctccttt ttaccgcgct gttcggagcc attttcccga ccttccggcc ttttgccggc
     8221 cacaatgttc atcagctgat cgcacatccc gccagttcgt catttccgag ccatcactcg
     8281 ttcagctcga tgagcctgtc tctgggaatg cttcgggtcc gaaaagggct gggcttaggg
     8341 atgattttga tttccgtact gatcggattc tcccgtgttt atgttggcgt ccattacccc
     8401 gccgatgttt tgggaggctt cctcttagcg ttcatctgcc acattgctta ccggaagccg
     8461 ataaaaaaga agattgaatc cgcctatctg aaactggagg aggccgttgt ttcaaaactc
     8521 cggccccgtt ccttct
//
LOCUS       DATALY010000118         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_544878,
            whole genome shotgun sequence.
ACCESSION   DATALY010000118 DATALY010000000
VERSION     DATALY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544878"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VHR42_03520"
     CDS             complement(<1..411)
                     /locus_tag="VHR42_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEX3026281.1"
                     /translation="MANGHGGARVGAGKKKKALADNLLDGNPGKRKLTVLEFSDAADL
                     DGQPMPPPRSYLSAKQKDGSQTLAVEVYERTWKWLNERGCSQLVPAQLIEQYAVSVSR
                     WIQCEECITEFGFLAKHPTTGNAMPSPYVAMSQSF"
     gene            complement(580..1185)
                     /locus_tag="VHR42_03525"
     CDS             complement(580..1185)
                     /locus_tag="VHR42_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX3026282.1"
                     /translation="MSALDELYLELNRHLLHDEAPSRYLNSICDEPVFQQYPFDMLLK
                     LKQAKQSPKYHPEGSVWNHTMLVVDEAAKQKVRSKNPNAFMWAALLHDIGKPAATRIR
                     KGKITSYDHEKIGEKLAGEFLRTFVNDDQFIKKVTSLVRWHMQILFVVNNLPFADIEE
                     MKHDVDIREIGLLGLCDRLGRLHADKEKEEQNMRLFLRKCN"
     gene            complement(1182..1313)
                     /locus_tag="VHR42_03530"
     CDS             complement(1182..1313)
                     /locus_tag="VHR42_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPC_1213 family protein"
                     /protein_id="HEX3026283.1"
                     /translation="MGNLKKQDKKEDGKFHSKNINHDPRTESAKAVFDSNDKNVNKK"
     gene            complement(1515..1871)
                     /locus_tag="VHR42_03535"
     CDS             complement(1515..1871)
                     /locus_tag="VHR42_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX3026284.1"
                     /translation="MPTKPKRPCSYPGCPELVAGRYCEAHQKLIDARYNRFQRDSATG
                     KRYGGEWRKIRGRYIKVHPLCEMCKKAGKLTPAEEVHHIIPLSKGGTHAESNLMSLCT
                     SCHSTITAREGGRWQK"
     gene            complement(1989..2387)
                     /locus_tag="VHR42_03540"
     CDS             complement(1989..2387)
                     /locus_tag="VHR42_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026285.1"
                     /translation="MNKKELSQLYFLNREIEEQQRRLRELEELAISCTVHITGMPHGT
                     GVADKIGKYAAEIADLRGLLDLNMKKCFYELNRLNRYISSIEDSQMRMILSLRYINGL
                     SWRQIAFSIGGGNTENSVRMQHSRFVDEKK"
     gene            complement(2380..>2563)
                     /locus_tag="VHR42_03545"
     CDS             complement(2380..>2563)
                     /locus_tag="VHR42_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEX3026286.1"
                     /translation="IACIGGRFFAFEVKTKSGKLTKLQEITIRRIQEAKGQAYKVTSL
                     SEVKSILENLEDEPYE"
BASE COUNT          609 a          643 c          549 g          762 t
ORIGIN      
        1 gaaggactgg ctcatggcaa catacggaga gggcattgcg tttccagtcg tcggatgctt
       61 tgcgaggaaa ccgaactcgg tgatgcactc ctcgcactga atccagcggg aaacgctcac
      121 ggcgtactgc tcaataagct gcgcgggcac aagctgagaa caaccacgct cgttcagcca
      181 cttccatgtt ctttcataca cctcaacggc aagcgtctgg ctgccgtcct tttgctttgc
      241 ggaaagatag ctgcggggtg gcggcatcgg ctgtccgtca aggtctgccg cgtcggaaaa
      301 ttccaatacc gtcagcttgc gcttaccggg attgccgtcc aacaagttat ctgccagcgc
      361 ttttttcttt tttccagcac caacacgtgc accaccatga ccgttcgcca tttaactcac
      421 cgcccctcta aaagaaattc ctatacccta acttgaaatt gcgaattttc gcgcgtgacc
      481 ccacgcccgt tgcaaaagga aaaaagtgac agggatttta ttaccccacg gggaataaaa
      541 atgcacctgt tccatcgtca aaaggaacag atgctcacgt taattgcatt ttctaagaaa
      601 cagccgcatg ttttgctctt ctttttcttt gtcggcatgc agccttccaa gtctgtcaca
      661 taaaccgagc aaaccaatct cgcgaatatc aacgtcatgc ttcatttctt cgatatcagc
      721 aaaaggcaga ttattcacaa caaacaggat ttgcatatgc catcggacaa gcgaagtcac
      781 tttctttata aactgatcat cattcacgaa ggttcttaag aactcaccgg caagcttctc
      841 ccctatcttc tcgtgatcgt aagaagttat tttccccttt ctgattcttg ttgcagcagg
      901 tttgccaata tcatgtagaa gcgcagccca cataaaagcg ttcgggtttt tgcttcgaac
      961 tttctgtttt gctgcctcgt ctacgacgag catcgtatgg ttccaaacac tgccttcggg
     1021 gtggtatttt ggagactgct ttgcctgctt cagcttaagg agcatatcga acggatattg
     1081 ttgaaatact ggttcatcac agatactgtt caagtaccgg gatggtgctt catcgtgcaa
     1141 cagatgccga ttcagttcga gatagagctc atcaagcgca ctcatttttt attgacgttt
     1201 ttatcattag aatcaaatac tgcttttgcg ctttccgtgc gcggatcatg gttgatgttc
     1261 ttggaatgga acttgccgtc ctcttttttg tcttgctttt ttaaattacc catattgatt
     1321 ctccctcatc gatttgtgtt tggcccaagg gaaacttttt tcgagttgtg attttgattc
     1381 agatttgttt ccttggcttg tgcggattca acaccatcct tcagtttttt ctggaaaccg
     1441 gatggatgtt ccgaatgctt tccagccatt ttactcacct cggaattatt gtgccattga
     1501 gaattttatt tattctattt ctgccatctg ccaccttcac gagcagtaat cgtcgagtgg
     1561 caggaagtgc aaaggctcat cagattactc tcggcatgcg ttccgccttt ggacaacgga
     1621 atgatgtgat gtacttcttc agcgggagtc aatttcccag cttttttgca catctcgcac
     1681 agaggatgga ctttgatgta cctgccgcga atctttctcc attcgccgcc gtatcgtttg
     1741 ccggtggctg agtcgcgctg aaatcggtta tatcgggcat cgatcagctt ttgatgtgcc
     1801 tcacaatatc ggcctgccac taactccggg caaccgggat aactgcaggg tcgttttggt
     1861 ttggtgggca taatggctat ctccttcaaa aaaagagcaa ggacacccac tgctttcggt
     1921 ggatgccctt gaggtaaact ttgatgaaac tactatagca catctaaacc gaacaaaacg
     1981 aacaactttc attttttctc atcaacgaat cggctatgct gcatccgaac gctgttttcc
     2041 gtattaccgc cgccaatgga aaaagcaatc tgccgccatg agagtccgtt tatgtaccgg
     2101 agcgaaagaa tcatccgcat ctgactgtct tcaatgctgc tgatataacg gttcagtcga
     2161 ttgagttcat aaaaacactt tttcatattg agatccagta agccgcgaag gtcagcgatc
     2221 tcagcggcat atttaccgat tttatcagcc acaccggttc catgcggcat accggtgata
     2281 tgcaccgtgc aggaaatcgc cagttcttcc agctcccgca gcctgcgctg ctgttcttca
     2341 atttcgcggt tgagaaaata aagctgggaa agctcttttt tattcataag gttcatcctc
     2401 caaattttca agtatggatt ttacttcact caaactcgta actttgtagg cttgaccctt
     2461 tgcttcctgg attcttcgta tcgtgatttc ctgtaacttt gtcagctttc ccgattttgt
     2521 cttgacctca aaggcaaaga atctgccgcc gatgcaggca atg
//
LOCUS       DATALY010000119         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_26949,
            whole genome shotgun sequence.
ACCESSION   DATALY010000119 DATALY010000000
VERSION     DATALY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26949"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VHR42_03550"
     CDS             complement(<1..682)
                     /locus_tag="VHR42_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018886868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3026287.1"
                     /translation="MEYTYSDRISALQPSLIREILKNSSGSNIIPFSAGNPSPESFPV
                     EAIRKISQEILTENPTEALQYGISEGYTPLRDALKIRMRQKFGCGRDFDDLVIVSGGQ
                     QGIELTCKVLCNEGDTVICENPSFIGALNAFRSYNVKLRGVKMEDDGVNTDELEKALK
                     EEKNVKLLYLIPTFQNPTGITMSLEKRKTVYALAKKYGVMILEDNPYADLRYSGNDVP
                     TIKSIDTEG"
     gene            824..2290
                     /gene="gltX"
                     /locus_tag="VHR42_03555"
     CDS             824..2290
                     /gene="gltX"
                     /locus_tag="VHR42_03555"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX3026288.1"
                     /translation="MCQLKGVRTRFAPSPTGYMHVGNLRTALYAYLLAKSSEDGKFIL
                     RVEDTDQERYIEGALDVIYKTLKETGLVWDEGPDIGGPVGPYVQTERREIYRKYAEEL
                     IEKGGAYYCFCDKDRLEELKKIQDASGMSHKYDGHCRNLSKEEIDELLKSGVPYVVRQ
                     KIPKEGTTTFHDEVFGDITVENETLDDNILLKTDGLPTYNFANVIDDHLMNITHVIRG
                     IEYLSSAPKYNLLYEMFHWEIPVYIHCPPVMKDSSHKLSKRNGDASYEDLIAKGYLKD
                     AVLNYIALLGWSPGGEQEVFSLEELTKVFRVSGISKSPAIFDTNKLDWINGEYIRALS
                     LENFHQCALPWIKKAVTRETDTRLIAEVLQPRCVKLSDIPEQLDFIDRLPEYSVELYR
                     HKKMKTNPENSLEALREVRPVLETVQDWTRENIHSALFDLIAQKGVKNGIILWPVRVA
                     VSGKQFTPGGGVEICMLLGKEESLCRIDKGIELLTPSV"
     gene            complement(2424..2720)
                     /locus_tag="VHR42_03560"
     CDS             complement(2424..2720)
                     /locus_tag="VHR42_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX3026289.1"
                     /translation="MVKHIVMWKLKDTAEGSPKKTNAIKIKKMLEALQGVVPGAYKME
                     VGINYNPGGYDVVLYSEFNDHDALESYQVHPEHIRVKEFVHSVASARVVTDYIV"
BASE COUNT          777 a          736 c          759 g          809 t
ORIGIN      
        1 acccttccgt atctatcgat ttaatggtgg ggacgtcgtt tccggaatag cgcagatcgg
       61 cgtaagggtt gtcttccagt atcattacgc cgtatttttt ggcaagcgca taaacggttt
      121 tccgcttctc aagagacatg gtgattccgg taggattctg gaaagtagga atcagataca
      181 gaagctttac gtttttttct tctttcagcg ccttttccag ctcgtcggta ttcacgccgt
      241 cgtcttccat ctttacgccg cgcagcttta cgttgtaaga gcggaaagcg ttgagcgcgc
      301 cgataaaact cggattttcg cagatgaccg tgtcgccttc gttgcagagc accttgcagg
      361 tcagctcgat tccctgctgg ccgccggaaa cgatcaccaa gtcgtcaaaa tctctgccgc
      421 acccgaattt ttgcctcatt cggatcttga gagcatctct gagcggtgta taaccttcgg
      481 aaatgccgta ctggagcgcc tcggtcggat tttccgtcag gatttcctgc gatattttcc
      541 ggatggcttc caccggaaaa gactcggggg acgggttccc ggctgaaaaa gggatgatgt
      601 tcgatccaga agaattctta aggatttccc tgatcaggga aggctgaagc gccgagattc
      661 tgtctgaata agtatattcc atcaaaatca tcctaaccat gataaaaaat acaatgttta
      721 accgtaattg taccacaata ggtttccctt gacaataact caggtgcgaa tgtattatat
      781 taggtacgag tgttttgctt gaaaattacc ggaggttgtt caaatgtgtc agttaaaagg
      841 ggtgcgcaca cgtttcgcgc ccagtcccac gggatatatg catgttggaa atttacgtac
      901 ggctctttac gcttacctgc ttgccaaatc atcggaagac ggtaagttta ttctgagggt
      961 ggaagacacc gatcaggagc gctatattga aggagccttg gatgttatat acaaaacact
     1021 gaaggaaaca ggccttgtct gggatgaagg cccggatatc ggcggcccag tgggccctta
     1081 tgttcagacc gaacggagag aaatctaccg caaatacgcc gaggagctga tcgaaaaagg
     1141 cggagcctat tattgcttct gtgacaaaga ccggctcgaa gaactcaaaa agattcagga
     1201 cgcgtccggc atgtctcaca aatatgacgg tcattgccgc aatctctcca aagaggaaat
     1261 cgacgagctt ctgaaatccg gcgttcccta tgtggtccgg cagaaaatac ccaaagaggg
     1321 gacaaccact ttccacgatg aggttttcgg cgacatcacg gtggagaacg agacgctgga
     1381 tgataacatc ctgctcaaaa ccgacggcct gcccacctat aatttcgcca atgtcatcga
     1441 cgatcacctg atgaacatta cccatgtgat ccgtgggatc gaatatcttt cctccgcgcc
     1501 gaaatacaat ctgctttatg aaatgttcca ttgggaaatt cccgtttata tccactgccc
     1561 gcccgtgatg aaggattctt cccacaagct ctccaaacgc aacggcgacg cttcttatga
     1621 ggacttgatt gccaagggct atctcaagga cgcggttctc aattatattg cgctgctggg
     1681 gtggagcccc ggaggggagc aggaggtctt ttcgcttgaa gagctgacca aagtgttccg
     1741 tgtgtcggga atctccaaat cgcccgcgat ttttgacacc aacaaactgg actggattaa
     1801 tggagaatat attcgcgcgc tcagtctgga aaacttccat cagtgcgcgc tgccctggat
     1861 caagaaagcc gtaaccagag aaacggatac ccggctgatt gcggaagtgc tccagccgag
     1921 atgcgtcaag ctgtcggaca ttccggagca gcttgatttc atcgaccggc ttcccgagta
     1981 ttccgtcgag ctttaccgcc ataagaaaat gaagaccaac ccggaaaatt cactggaagc
     2041 gcttcgggaa gtcaggccgg ttctggagac ggttcaggat tggacgagag agaacatcca
     2101 cagcgccctt tttgatctga ttgctcaaaa gggtgtaaaa aacgggatta tcctgtggcc
     2161 tgtccgcgtc gctgtttccg gtaaacagtt tactcccggc ggcggtgtgg aaatctgcat
     2221 gctgcttgga aaagaagagt cgctgtgcag gattgataaa ggaatcgagt tgttaacccc
     2281 ttcggtgtaa acagccctga agggacggca agaagcggtt tgaggaaact caaaccgctt
     2341 cttttcttac acttttctat tttatccgta tccgggcggg ctcacggctc tttccgcgga
     2401 tatgtagccg ctgcgccggc ttctcagaca atataatcgg ttacgacgcg tgccgaggcg
     2461 acgctgtgga caaactcctt aacgcggata tgctcaggat gaacctgata gctttccagc
     2521 gcgtcgtgat cattgaactc ggaatagagc acaacgtcat agccgccggg attgtaattg
     2581 attccgactt ccattttgta agcgccggga acaacgccct gaagagcctc aagcatcttt
     2641 tttatcttta tggcatttgt ctttttgggt gatccctcgg cggtatcctt aagcttccac
     2701 attacaatat gcttgaccat gtatatcctc cctgctttct attagaatct gtaaaacctg
     2761 atcaaataat tcggctgtta tgagaatggg gcatctgtat tttgtcagcg taatgaaagc
     2821 cggtcggatc gcccgaattt ctcttcgctc acatgctttg gctatttttt ttgaaatatc
     2881 ttcaaattcg tattgattct gcgaatggtt tggtgtaaat tttaatatat tttatttatc
     2941 atttccccaa agcaggtgag cgaagagaaa ttcgggcggt ccgaccggct ttcattacgc
     3001 tgacaaaata cagacgcccc attctcataa cagccgaatt atttgatcag gttttacaga
     3061 ttctaacaga aagcagggac a
//
LOCUS       DATALY010000120         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_803397,
            whole genome shotgun sequence.
ACCESSION   DATALY010000120 DATALY010000000
VERSION     DATALY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803397"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="nth"
                     /locus_tag="VHR42_03565"
     CDS             <1..441
                     /gene="nth"
                     /locus_tag="VHR42_03565"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX3026290.1"
                     /translation="PIIRSCGLYKTKARDIVKLCRTLLNEYHSQIPDTVEELIKLPGV
                     GRKTANLVVGDIFGKPAIVTDTHCIRLSNRLGLADSKDPYKVELQLKKLIPPEQSTLF
                     CHRLVWHGRAVCTARNPACERCILMELCKNLPEKKELDKTGPNR"
     gene            477..552
                     /locus_tag="VHR42_03570"
     tRNA            477..552
                     /locus_tag="VHR42_03570"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:510..512,aa:Ala,seq:cgc)
     gene            complement(919..1536)
                     /locus_tag="VHR42_03575"
     CDS             complement(919..1536)
                     /locus_tag="VHR42_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3026291.1"
                     /translation="MKIVVVDDDKLVSASLKTIIETDSEIQVAGIGYSGADAIELFET
                     HAPDILLMDIRMDGMTGLEAGEAILRRHCHAKILFLTTFADDEYIVRALKIGAKGYML
                     KQNFESIVPSLKAVSIGQSVFGDEIVAKIPSLISGEKTDLKKYDISEKEEEIIRLISE
                     GLSNKEIANALYLGEGTVRNSISVILDKLHLRGRTQIAIFYFKNK"
     gene            complement(1541..>1869)
                     /locus_tag="VHR42_03580"
     CDS             complement(1541..>1869)
                     /locus_tag="VHR42_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026292.1"
                     /translation="NFSFCSLSLDYDIPGNPDKKYKYAFLSILKEALANIIKHSNATS
                     VRVSLREHPALYQLVVKDNGTLESRQKEAGLGLKNIANRVEGLGGIVNFSSENGFTIF
                     ISIPKE"
BASE COUNT          456 a          475 c          421 g          517 t
ORIGIN      
        1 ccgatcatcc gctcctgcgg tctttataaa acaaaagcca gggacattgt aaaactctgc
       61 cggacgcttc tgaatgaata ccattcacag attcccgata cggtcgagga actgataaag
      121 ctgccggggg ttgggcgcaa aactgcaaat ctggtcgtgg gcgacatctt tggcaaaccg
      181 gccatcgtta ccgacacgca ctgcatccgt ctttccaacc ggctgggtct tgccgattcg
      241 aaggaccctt acaaagtaga actccagctg aaaaagctga ttccaccgga acaatcaacc
      301 ttgttctgcc accgcctggt ctggcacggc cgggcagtct gcaccgcgag aaatccggcc
      361 tgcgaacgct gtattctcat ggaactttgc aaaaatcttc ccgagaaaaa agagcttgac
      421 aaaacaggcc cgaaccgata aaataatatg gcaggttctg tgtaacctgc tgtttcgggg
      481 gcgtagctca gctgggagag cgtacggttc gcatccgtaa ggtcgagagt tcgatcctct
      541 tcgtctccac catcaaaaag tcggcaacta tatggttgcc gacttttttt atgttcggga
      601 agtctccgct tttgggggcg caaccaaaat ttttctacta tctgattatc atatctttaa
      661 cggaggaatt ggtcttcaga ggatatattc agtcaagaat ttttgcacca actctatttc
      721 atgctttaat ggattggagc aatattttat ttttataaca ggctcttgcc ataacgctct
      781 attccctgca tcttttatgg ccgaattttc cacacaaaag ccaacaacca ttcgggttgc
      841 tggcttttgt gtttatggct aaatcggggg aatctaacga tcttatcata aggcagccct
      901 gcggcgcttt ataatacctt acttattttt gaaataaaaa attgcgattt gcgtcctgcc
      961 gcgcaaatga agcttatcca gaatgacgct gatgctgttg cggacggtac cttcgcccag
     1021 atacagagcg tttgcgatct ctttgttgct caagccctcc gagatcagcc ggataatttc
     1081 ctcttctttt tcactgatgt cgtatttctt aagatcggtt ttttcgccgc tgatcagcga
     1141 ggggattttg gcgacaattt cgtccccgaa cacactttgg ccaatactta cggccttcag
     1201 ggatgggaca atactttcga aattctgctt gagcatgtag ccctttgcac cgatttttaa
     1261 ggctctgaca atatattcgt cgtccgcgaa ggtggtcaga aacaaaattt ttgcatggca
     1321 atgcctgcgc agaatggcct caccggcctc aagccccgtc ataccgtcca tccggatatc
     1381 catgagcagg atatccgggg catgggtttc aaacagttca atcgcgtccg cgccgctgta
     1441 cccgataccg gccacctgta tctccgaatc cgtttcgatg atcgttttca gcgaggcgct
     1501 gaccagcttg tcatcgtcca caacaacaat tttcatgatt ttactccttt ggaatcgaga
     1561 tgaagatggt gaatccgttt tcgctgctaa aatttacaat gccgcccagc ccctccaccc
     1621 ggttcgcaat gtttttgagc cccagtccgg cttctttctg ccggctctcc agcgtaccgt
     1681 tatccttgac aaccagctga taaagggccg gatgctcccg caaagaaact ctcacggaag
     1741 tggcgttcga gtgtttgatg atattcgcca aagcttcttt tagaattgaa agaaatgcgt
     1801 acttatattt tttatccgga ttgcccggaa tatcgtagtc aagcgacaga gagcagaagc
     1861 tgaaatttt
//
LOCUS       DATALY010000121         9208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_372794,
            whole genome shotgun sequence.
ACCESSION   DATALY010000121 DATALY010000000
VERSION     DATALY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372794"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VHR42_03585"
     CDS             <1..250
                     /locus_tag="VHR42_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026293.1"
                     /translation="LDSRIDKIEKEIDRLNSKYNEVTVGQAAIAEKINCVLVTMTEVK
                     ESIKAIQQIPSKRWETLITAVISAAVVTIIGFLAGKFL"
     gene            complement(273..2606)
                     /locus_tag="VHR42_03590"
     CDS             complement(273..2606)
                     /locus_tag="VHR42_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX3026294.1"
                     /translation="MNSTYRLNNTTRNPYLETFILAFLIALSILLPYMILDKGLFLFY
                     GDYCVQQVPFYSLAHDAIRSGNVLWNWNTDLGANFVGSYAFYLLGSPFFWLTIPLPSF
                     LSPYMLGPLLALKIATAAVTSYAFIRRFSKTHEFAALGALLYAFSSYSIYNIFFNHFH
                     EPMAFFPLLLIALEELMIHNRKAVFALAVCLNAFVNYIFFVSEVVFLILYFSLRLASG
                     DWKITRRKFFSLAFESIIGVGISAVLLLPAVMAIINNPRTSTLLSGWNLLLYNWNQRY
                     PDILHSLFFPQDLPSRPNFFPDSNANWSSVAAWLPMFSMTGALSFIIAKRGHWLKRIL
                     CVSFVMAMIPGLNALFVLLNDDYYARWFYMPVLMLCLATVIAFEDPEIDIKPGLRWTF
                     SVTAAFALAIGLIPKVENGTIKLGLEQYPDKFIAYVAIVILGLAALFFLSRKYGKHTI
                     GFANASTIALALIICIYGNFFIATGKALGWNGSWFKSTAVEGASKIKLDETRFYRIDV
                     LNGIDNQGMFWKLPTINAFQSVVPASIMEFYDSIGLQRDVASRPSENFMGLRSFLSVR
                     YVFDSQNNASLTMPGWVKYKNELGFTVWKNSNYIPMGFTYDRFVNQKQYRSSGFKDML
                     LLKAIVLNNEQIAKYSSILSLYNTADADFSDTTLAADCAARRREACSDFKTTNSGFTA
                     DINLSKENLVFFSVPYDSGWSATVNGKQSNIEKVNVGFMAVKCGKGGNSIRFTYKTPG
                     LSAGFVVSLASLTALILYLLFNRLKSYKPPRKTKRLA"
     gene            2839..3459
                     /locus_tag="VHR42_03595"
     CDS             2839..3459
                     /locus_tag="VHR42_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX3026295.1"
                     /translation="MADKPGEEIMAGSVLLEWIAEYGLAAVFIMIAMEYACFPMPSEI
                     LLPFAGAFAAQNGIGFFSLLLVSVGAGITGAFTCYLIGAFGGSAVIDRISRKHPKTAA
                     GINASRAWFHRYGNLSVMIGRVLPICRTYISFVAGISRQNPVWFAAFSLIGISIWNLV
                     LTGLGFMLGDNWERVSVYAEKYKYILLPVVLVLIFGIINHIRNKRK"
     gene            3471..4055
                     /gene="yfbR"
                     /locus_tag="VHR42_03600"
     CDS             3471..4055
                     /gene="yfbR"
                     /locus_tag="VHR42_03600"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HEX3026296.1"
                     /translation="MNSGHFFAVVSRMKFITRWGLMRNTRPENLEEHSLEVAILAHAL
                     AVIKNRRFHGNVDADHIAVLAMFHDVSEIYTGDLPTPVKYHDDELVAAYKKVESLSAK
                     KLLNFLPEDLRGDYRGLILQEEPDDNLWKIIKAADKLSALIKCLEEEKAGNSEFKKAA
                     SAQIEYLKEMRLPEVDCFMKEFLPSFRLTLDELG"
     gene            4127..4720
                     /locus_tag="VHR42_03605"
     CDS             4127..4720
                     /locus_tag="VHR42_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX3026297.1"
                     /translation="MNHHKTLWITTTAVLIALVVLAQLLTYAIPAGALIPTPLGPIRL
                     SQFFTGSLVNLILIVGAGAAGFGSASIAALISPVLAALLGVIPGGIPQMIPVVMIGNL
                     IIVAIVWLCFKISTDLVRAGGIVLKILGVIVGAAAKAVFLWAATAKLIVPLFNVKPKI
                     AAQIGILMSWPQAVTALVGGILALMVLPALKIGAKNR"
     gene            complement(4820..6040)
                     /locus_tag="VHR42_03610"
     CDS             complement(4820..6040)
                     /locus_tag="VHR42_03610"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX3026298.1"
                     /translation="MSLIVQKFGGTSVANAERVNYVAGIVTDTYKAGNSVVVVVSAQG
                     DTTDDLISKAAEINPNPSKREMDVLLSTGEQISMSLLAMAIQKMGYPVVSLTGWQAGL
                     KTDYSYGNARIESIDSGRIQMEIDKGKIVIVAGFQGINKYDDITTLGRGGSDTTAVAF
                     AAELKADLCQIYTDVDGIYTTDPRIVPNASKLKEISFDEMLELASLGAQVLHNRSVEM
                     AKKYNVNLEVLSSFKKAPGTKVMEVINMEKMLVKGVARDNNVARISVIGVPDQPGIAF
                     KIFSMLAQKKINVDIILQSIGRSNTKDISFTISQNHLETALNLLNENLPVIKGSSVAY
                     DDNISKVSIVGAGMATNPGVAAKMFESLYESGINIQMIATSEIKISVLINRSEAEKAV
                     NVIHEYFHLGSLMA"
     gene            complement(6247..7137)
                     /gene="thrB"
                     /locus_tag="VHR42_03615"
     CDS             complement(6247..7137)
                     /gene="thrB"
                     /locus_tag="VHR42_03615"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX3026299.1"
                     /translation="MLKIQVPATSANIGPGFDSLGLALRLYNRVFMEEYDGVRISTLD
                     GSVVPQDEENLIYKSARFFYDLCGKPFRGLKIEQENNIPMTRGLGSSSACIVAGIVGA
                     NALLGHPLSKDDLVNLAAVLEGHPDNSTPAILGGLTAAVLQENHVYSVKVPISGRIKF
                     AAFIPDFELKTETARAALPSEISHKDAVFNVSRAALMTASLFSGRLDNLRVAVEDKLH
                     QPYRLKFIPGADGIFDLAARLDAYATYISGAGPTIISMVNVTDLGFKDKAERELKKTH
                     PGWRLVMLDCDEDGATSEEV"
     gene            complement(7253..8497)
                     /locus_tag="VHR42_03620"
     CDS             complement(7253..8497)
                     /locus_tag="VHR42_03620"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX3026300.1"
                     /translation="MIKIAVLGHGVVGSGVLEVFTKNHKSIIAKAGENMEIKYILDLR
                     KFPDLPYSDKFVQTFDPIINDPEINLVVEVMGGVNPAYDYVKRCLLAGKNVVTSNKEL
                     VATYGSELLEIAHEKNINFLFEASVGGGIPIIRPISQCLAANELNEVAGILNGTTNFI
                     LTRMISDSMSFDDALALAQKLGYAEHNPAADVEGLDACRKICILASLAYGRHVNPKDV
                     HTEGISKITLEDVKYAAAWGGVIKLIGRAKKLPDGRLSAMVCPAFIKKSSQLAGIDDV
                     FNGIMVRGDAIGDVVFYGRGAGKLPTASAVVADIIDVAKHLKARKYLYWLPPKDGYVC
                     NYLEAPSVFFVRVRTQDRLTAIKAANSLFGKVSEMVHPDMPAGEAAFITPELPEGELN
                     RAIASLSSLDYENIACIRVLDY"
     gene            complement(8526..8969)
                     /locus_tag="VHR42_03625"
     CDS             complement(8526..8969)
                     /locus_tag="VHR42_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX3026301.1"
                     /translation="MDGMPRYLLVDCEVLPEVFHKVVEAKRYLASGQVHSVNEAVRLA
                     GISRSAFYKYKDKVHPFNNKDGGRIITLHTMLRDAPGVLSELIGRLYRSGANILTINQ
                     NIPIGGVAPVSVSARIDGLRIPLEEMLVLLREIDGVENIEIVSGQ"
     gene            complement(8971..>9208)
                     /locus_tag="VHR42_03630"
     CDS             complement(8971..>9208)
                     /locus_tag="VHR42_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX3026302.1"
                     /translation="DREKCIHCGSCLEVCCHDSRLFTDDTEDVLNHIHNGKGFIVLAA
                     PSIRVNFKDSWERLISTFKQLGAKAVYDVSVGAV"
BASE COUNT         2474 a         2154 c         2216 g         2364 t
ORIGIN      
        1 actggattcc agaattgaca aaatcgaaaa ggaaattgac agattaaatt caaaatacaa
       61 tgaggttacg gttggacagg ctgcaatagc agaaaaaatt aattgcgtac tggttaccat
      121 gactgaagtt aaagagtcaa tcaaggccat acagcagatc ccgtcaaaaa gatgggaaac
      181 cttaattaca gctgttattt ctgcagccgt tgtaaccatt atcggttttc tcgcaggaaa
      241 atttttgtaa attagaattc aatcaaattg atttaggcaa gccgcttcgt tttacggggc
      301 ggcttgtatg attttaggcg gttgaataat aaataaagaa tcagggcggt taaggaagca
      361 aggctgacta cgaacccggc tgacaagcca ggggttttat aagtaaaacg tatggagttt
      421 cccccttttc cacatttaac agccataaag ccgacgttaa ctttctcaat attcgattgc
      481 ttaccattaa cagtcgccga ccatccgctg tcgtatggta cgctgaaaaa gaccagattt
      541 tcttttgaaa gattgatatc agcagtaaat ccgctattcg ttgtcttaaa atcagagcag
      601 gcttcgcggc gcctcgcggc gcagtcggca gccagcgtag tgtcactgaa atcggcatcg
      661 gcagtattat agaggctcag aatggacgag tactttgcaa tctgctcatt attcagaacg
      721 atcgctttga gcagcagcat atctttgaag ccggatgaac gatactgctt ttgattgaca
      781 aaccgatcat aagtaaagcc catggggata tagttgctat ttttccagac cgtaaaaccc
      841 agttcattct tatattttac ccaaccgggc attgtaagac ttgcattatt ctgcgagtcg
      901 aacacatagc ggacagataa aaacgatctc aggcccataa agttttcact cggacgagag
      961 gccacatctc gctgaagccc aatcgaatcg taaaattcca tgattgaggc cggtacgacg
     1021 ctttgaaatg cgttaatcgt cggcagtttc caaaacatac cctgattgtc aatgccattc
     1081 aggacgtcaa tacgatagaa tcgcgtttcg tccagtttga tttttgaggc accttcgacg
     1141 gctgttgatt taaaccagct gccgttccat ccaagggcct ttccggttgc tataaagaag
     1201 ttgccataga tacaaattat cagagccaac gcaattgtag aggcgttcgc aaagccgatc
     1261 gtatgctttc cgtattttct tgagagaaag aataaagcgg cgagcccgag tatgacaatt
     1321 gcaacataag cgataaattt atcaggatat tgttcgagac caagttttat agttccgttt
     1381 tctacttttg ggatcagccc gatcgccagc gcaaaggccg ccgtaacgga aaaagtccat
     1441 cgaagaccgg gcttaatatc gatttcaggg tcttcaaaag cgataaccgt ggccaggcac
     1501 agcatgagaa ccggcatata aaaccaccgt gcgtaataat catcgtttag cagcacaaac
     1561 aaagcattca gccccggaat cattgccata acaaaactga cgcaaagaat tctcttcagc
     1621 cagtgtcccc gtttggcaat gataaaagaa agagcgccgg tcattgaaaa catcggcagc
     1681 catgcggcga cggatgacca gttggcattt gaatccggga aaaagtttgg ccgcgaaggc
     1741 aagtcctgag ggaaaaacag agaatgcaga atatcgggat atcgctgatt ccagttgtac
     1801 aggagcaggt tccatcccga caggagggtg gaagtccttg gattattgat gatagccata
     1861 acggcgggca gcagcaatac agcgctgatc cccactccta taatcgactc aaacgcgagc
     1921 gagaaaaatt ttctgcgagt aattttccaa tctcccgaag cgagcctgag ggaaaaatag
     1981 aggatcagga agacgacttc tgaaacaaag aaaatataat ttacaaaagc gtttaggcaa
     2041 actgcaagag caaaaaccgc cttgcggtta tggatcatta actcttcaag agcaattagc
     2101 agaagcggaa agaacgccat cggttcgtga aagtgattaa aaaagatgtt ataaattgag
     2161 taactggaga aggcgtacag cagcgcgccg agggcggcga attcgtgagt tttggaaaac
     2221 ctgcggataa aggcatagga ggtcactgcg gctgtggcta tttttaatgc gagcagaggg
     2281 ccgagcatat aaggactaag aaaggatgga aggggaattg tcagccaaaa aaaaggactt
     2341 cctaaaagat aaaatgcata cgacccgaca aaattagctc ccagatcggt gttccagttc
     2401 cagagaacat tgcccgaacg gattgcgtca tgcgcgaggg aatagaaagg aacctgctgg
     2461 acgcagtaat cgccataaaa aagaaaaagc cccttatcca gaatcatgta ggggagcaag
     2521 attgacagag cgataagaaa cgcaagaata aaggtttcaa ggtatgggtt ccttgttgta
     2581 ttattgagcc gatatgtcga attcatcttg ttaccccgca tttcggtgag agtaggtggt
     2641 ttcaaccaac atctaaatgt aagaaatatc ctagaaaaat attataacat taatcaggct
     2701 ggaatgaaat tcgttttttt gaatatttta ttaatattaa aatggtaggt ccatccattt
     2761 tcggaaggcg gcgctgttag acatttcggt cggtttttgc tataataaaa acaaacagcc
     2821 tgtcccagag gccggaatat ggcggacaaa ccgggggagg aaataatggc cggttctgtt
     2881 ctactggaat ggattgcgga atacgggctt gcggcggtct ttattatgat cgccatggaa
     2941 tacgcctgct ttccaatgcc cagtgaaatt ttattaccct ttgcgggagc gttcgccgcc
     3001 caaaacggca taggcttttt ttcgcttctc ctggtcagcg tcggagctgg tataaccggg
     3061 gcatttacct gttatctgat aggagcattc gggggaagcg cggtgattga cagaatatcc
     3121 cgcaagcatc caaagactgc ggcggggatc aacgcttccc gcgcatggtt tcaccgatat
     3181 ggaaaccttt ctgttatgat cggccgcgtc ctgcccattt gtcgtactta tatttcattt
     3241 gttgccggga tatcccggca gaaccctgta tggtttgccg ctttttcatt gattgggatt
     3301 tctatttgga atcttgtgct taccgggctt ggttttatgc tcggcgacaa ctgggaaaga
     3361 gtcagcgttt atgccgaaaa atataaatat attcttcttc cggttgtgct ggttctaatt
     3421 ttcggaatta taaaccatat cagaaataaa agaaaatgag tgaggcacct atgaatagcg
     3481 gccatttttt tgctgttgta tcgcggatga agtttattac tcgatgggga cttatgcgca
     3541 ataccagacc ggaaaatctc gaagagcaca gtttggaagt tgcgatactg gcgcatgctc
     3601 tcgcagtgat taaaaacagg cgttttcacg gaaatgtgga cgcggaccat atcgcagtgc
     3661 tggccatgtt ccacgatgtt tccgaaatct atacgggtga tttgccgacg ccggtgaaat
     3721 atcatgatga tgagctggtt gccgcttata aaaaggtgga gagcctttct gcaaagaaac
     3781 tgttgaactt tttgccggaa gatctgcgcg gagactaccg gggtcttatt ttacaggaag
     3841 aacctgatga taatctgtgg aagattatca aagcggcgga caagctttcg gctttgataa
     3901 aatgcctgga ggaagaaaag gcgggaaatt cggaatttaa aaaagcggca tctgcccaaa
     3961 ttgaatattt gaaagaaatg cggttgccgg aggtggattg ctttatgaag gaatttttgc
     4021 cgagcttcag actgacactg gatgaactgg gataatgaag tttcttatcc cgaagcggct
     4081 attgaagggg tatatggaaa ttaatcagca tgaggagttg cataaaatga atcatcataa
     4141 gacactgtgg ataacgacga cggcagtctt gatcgccctg gttgttttgg cgcagctatt
     4201 gacctatgcg ataccggcgg gagcactgat tcccacgccg ttgggcccga taaggctcag
     4261 ccaatttttt accggttcac tggtcaacct gattctgatc gttggcgcgg gcgccgcggg
     4321 ttttggctcg gcgtcaatcg ccgcactgat atctccggtt cttgcggcgc tgcttggcgt
     4381 cataccgggc ggtataccgc agatgatccc cgtggttatg atcggcaatt taataatcgt
     4441 tgcaattgtc tggctttgtt ttaaaatttc gactgattta gtcagagccg gcgggatagt
     4501 cctgaagatc ctgggcgtga tcgtcggagc ggccgctaaa gcagtttttc tctgggctgc
     4561 cacggctaag cttattgttc ctctttttaa cgtcaagcca aaaatcgccg cccagattgg
     4621 cattctgatg tcctggccac aggccgttac cgctctggtg ggcggaatac ttgcgctgat
     4681 ggtgctgccc gctttgaaaa taggcgccaa aaaccggtag aaataccgac agatcggtaa
     4741 gttgaccccg taaccctcaa ataacaaaaa ggctcccgaa gcggattgaa ccgcccggga
     4801 gctttgtgtt ttctatcatt taagccatca gtgagccaag atgaaaatac tcatgaataa
     4861 cattgaccgc tttttccgcc tccgaacggt tgatcagaac ggaaatttta atctcacttg
     4921 tggcgatcat ctgaatatta attccggatt cataaagcga ctcaaacatt ttcgcggcga
     4981 ctccgggatt ggtggccatt ccggctccaa cgatcgacac ttttgaaata ttatcgtcat
     5041 aggccacact gctgccctta atgaccggca gattttcgtt taacaggttc agagcggttt
     5101 ccagatgatt ctgcgatatg gtgaaactga tgtcttttgt attactgcgg ccaatagact
     5161 ggaggataat atcgacgttg atttttttct gagccagcat tgagaatatt ttaaatgcga
     5221 ttccaggctg atccggaact ccaatcaccg aaatccgagc gacgttgtta tcccgggcga
     5281 cgcctttgac aagcattttt tccatattta ttacctccat gacctttgtt cccggcgctt
     5341 ttttaaaact ggagagcact tcaagattga cattgtattt tttggccatt tcaaccgacc
     5401 ggttgtgaag gacttgtgcg ccgagggagg ccagttcaag catctcatca aaactgattt
     5461 cctttaattt cgacgcattg gggacgattc tgggatcggt ggtgtaaatt ccgtcaacat
     5521 cggtataaat ctgacacaga tcggctttca gctcagctgc gaaggcaacg gccgtagtgt
     5581 cggatcctcc ccgcccaagc gtggtaatgt cgtcgtactt gttgattccc tgaaatccag
     5641 cgacgatgac gattttaccc ttgtcaattt ccatttgaat tctgccgctg tcaatgcttt
     5701 cgattcgcgc gtttccatag gaataatccg ttttcagtcc tgcctgccag cctgtgagag
     5761 aaacgacggg atagcccatc ttttgaattg ccattgccag cagcgacatg gaaatctgct
     5821 caccggtaga aagcagaaca tccatttccc tcttggaagg gttgggattt atctcggctg
     5881 cttttgaaat cagatcatct gttgtatcgc cctgagctga cacaaccacg acgacactgt
     5941 ttccggcctt gtatgtatct gtaacaatac cggcgacata gttaactctt tcggcattag
     6001 cgaccgaagt gccgccgaat ttctgaacaa tcagactcat taataaccct ccatcaatat
     6061 tcaaggccag caataggata acccggttcg ccacaattat ccaaggtggg acggatcgcc
     6121 gtcattgcag aaagatcaca gagcatgcaa caaaaagcaa gatggacccg aattgacagt
     6181 gctgcaaact cttgactggc cttgttcccg cggcttcgga aaatcctgtt ttcctgatac
     6241 tgcagtttaa acttcttctg atgtggctcc gtcttcgtca cagtcgagca tgacaagccg
     6301 ccagcccgga tgcgtttttt ttagttccct ctcagcctta tccttaaagc cgagatctgt
     6361 gacattaacc atagaaataa tagtcgggcc tgctccgctg atataagtgg cgtaagcgtc
     6421 aagccttgcg gcaagatcaa agatcccatc cgcccccgga ataaacttca gccgataagg
     6481 ctgatgaagt ttatcttcca cggcgacacg caggttgtct aaccttccgg aaaagagcga
     6541 ggctgtcatt aaagccgccc gggagacatt gaatacggcg tctttgtgag aaatctcact
     6601 tggaagcgcc gcacgggcgg tttccgtttt taactcaaaa tccggaataa aggcagcaaa
     6661 tttgatacgg ccggatatcg gaacttttac actataaaca tggttttcct gcaatacggc
     6721 cgccgtcagc ccaccgagaa tggcgggcgt cgaattatcc gggtggcctt ccaaaaccgc
     6781 cgcgagattc accagatcgt ccttggacag cggatggcca agcagggcgt ttgcgcctac
     6841 gatgccggcc acgatacagg cggaactgct gccgagcccc cgggtcatcg ggatattgtt
     6901 ctcctgctct attttaagac cgcgaaatgg tttgccgcat aaatcataaa aaaatctggc
     6961 gcttttatat atgagatttt cctcatcctg aggcaccacc gagccgtcga gagtggagat
     7021 gcgcacgccg tcgtattctt ccataaaaac ccggttataa agcctgagcg ccaaacccag
     7081 tgaatcaaag cccgggccga tgtttgcgct tgtggccgga acttgtattt ttaacataag
     7141 ttttgctccc ttaacattgt atgttatttt aacacagaac gaaatagcaa atcaacgaca
     7201 attttcacaa taattgccga tcctgccata cagcccaaat ttatttgtcc tgttaatagt
     7261 caaggacacg gatacaggcg atattttcat aatccagaga ggataaggag gcgattgcgc
     7321 ggttcagttc gccttcgggc agttccgggg taataaaggc cgcttctccc gcgggcatgt
     7381 cggggtgcac catttcggaa acttttccaa agagggagtt tgctgcctta atggcggtta
     7441 accggtcctg tgtgcgcacc cttacgaaaa ataccgatgg agcctccaga taattgcaga
     7501 cataaccgtc cttcggcgga agccagtaga gatacttgcg ggctttaaga tgtttggcta
     7561 cgtcgataat atcggcgacc actgcgcttg ccgtcgggag ttttcccgcg ccgcggccgt
     7621 agaaaacaac atcgccaatc gcgtcgccgc gcaccatgat gccgttaaat acgtcgtcaa
     7681 tccccgcgag ctgactggat ttcttaataa aagcggggca gaccatagcg gacagccgac
     7741 cgtcaggcag ctttttggcc cggccgatca acttgatcac tccgccccag gccgccgcgt
     7801 atttaacgtc ttcaagggtt atctttgaga ttccctcggt gtggacatcc ttcgggttga
     7861 catgacgccc ataggcgagg gaggcgagaa tgcaaatttt acggcaggcg tccagcccct
     7921 ccacatcggc ggccggatta tgctccgcat aaccaagctt ttgtgcgaga gcgagagcat
     7981 catcaaagct catgctatcg ctgatcatcc tggtcagaat gaaatttgtc gttccgttta
     8041 aaatgcccgc tacctcattg agctcgttcg ccgcgagaca ctggctgatt gggcggatga
     8101 tggggatgcc gccgccaacg ctcgcttcaa acaggaagtt aatgtttttt tcatgggcaa
     8161 tttcaaggag ctcagagcca taagtggcaa ccagctcctt atttgaggtc actacgtttt
     8221 tcccggccag cagacagcgt tttacataat cataagcagg gttaacgccg cccatgacct
     8281 caaccacaag attaatctcc gggtcattga tgatcgggtc aaaagtctgg acaaatttgt
     8341 cgctgtaagg caaatcagga aatttcctca gatcgaggat atatttgatt tccatgtttt
     8401 cccctgcttt ggcaatgatg cttttgtggt ttttggtgaa cacctcaagg actccggagc
     8461 cgacgacgcc gtggcccaga acagctattt ttatcataaa attaccctcc gtaaagataa
     8521 accggttatt ggccggatac aatttcaata ttttccactc cgtcaatttc gcgcagcaga
     8581 accaacattt cctcaagagg aatccgaagc ccgtcaatcc gtgccgaaac cgataccggg
     8641 gcaacgcccc cgatcgggat attctgatta atggtaagaa tatttgctcc gcttcggtaa
     8701 agtctgccga tcagctccga caagaccccg ggcgcatccc tgagcatggt gtgcagggta
     8761 atgatgcggc ccccgtcctt gttattaaaa ggatggactt tatctttata tttgtaaaat
     8821 gcactcctgg atatccccgc gagcctgacc gcctcattga ccgaatgtac ctggccagag
     8881 gccagatatc gcttggcttc cacaacttta tggaaaacct ccggaaggac ttcgcaatcc
     8941 accagaagat aacggggcat tccgtccatc tcaaaccgcc cccaccgaca catcatagac
     9001 ggctttggct cccagctgct tgaaggtcga gatcagcctt tcccaactat ctttaaaatt
     9061 aacgcgaatt gacggcgcag ccagaacaat aaagcccttc ccattatgta tatggtttaa
     9121 aacgtcctct gtgtcatctg taaacaggcg ggaatcatgg cagcaaactt ccaagcaaga
     9181 cccgcaatga atacattttt cccggtcc
//
LOCUS       DATALY010000122         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_201848,
            whole genome shotgun sequence.
ACCESSION   DATALY010000122 DATALY010000000
VERSION     DATALY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201848"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VHR42_03635"
     CDS             <1..260
                     /locus_tag="VHR42_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026303.1"
                     /translation="VETLSVRVDKLERDTAELFEKTNTAAVSLAAVNEKLNSLLVTLG
                     EVKQAVSNLRDIPAKRLENILKALATAVLSGITGYILARFR"
     gene            269..559
                     /locus_tag="VHR42_03640"
     CDS             269..559
                     /locus_tag="VHR42_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026304.1"
                     /translation="MKTMFERNVEDMNLKIDWERKLTSRKFWTALCGFTAALLTAFHV
                     PAGTAAQVTSIIMAGASLIAYILSEGLADAAASSAQNGATNVPDPLPGAEQK"
     gene            717..2126
                     /gene="rmuC"
                     /locus_tag="VHR42_03645"
     CDS             717..2126
                     /gene="rmuC"
                     /locus_tag="VHR42_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX3026305.1"
                     /translation="MQYLILGACLITTVLSFVTMMAAFSLKKNQGGNMEDYRRMESLK
                     REILDEMRSNRQETSQSIQSSIRSMSELLVKTQTQTMSIQDTRLAELNRQLTLRQDAF
                     QKTMGETLGRVDKRMSELAGQTEIRLETIRTTMETKLTSMQEDNTKKLDQMRATVDEK
                     LEKTLQDRIGQSFRLVSERLEQVYKGLGEMQSLATGVGDLKKVLSNVKTRGVLGEVQL
                     GAIFEQILSPEQYCRNIATKRGSTCVVEYAVKLPGDGSGEVLLPVDAKFPADAYAQLT
                     DAYDGGDPALIQAAGSMLERRIKLFAKDIRDKYIDPPATTEFGVMFLPFEGLYAEVVR
                     RGLLETLQRDYKVSIAGPTTMAALLNSLQMGFRTLAIQKRSGEVWQVLGAVKTEFEKF
                     GGVLQAAQQKLEQANSELDKLVGVRTRQIQRKLQSVSALPEQESELLIDSDSSAAVRE
                     SAIFSADSSGGNDQENQEV"
     gene            complement(2159..>2945)
                     /locus_tag="VHR42_03650"
     CDS             complement(2159..>2945)
                     /locus_tag="VHR42_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3026306.1"
                     /translation="VIGASGYLGKTIYKKLSEKMANVSGTCNSHNTPGLIRVNLTNPT
                     DIQKIYLQKPDVIIWAVCDRTNELTLSQTGLQKLIQLIPETTRFVYISSKLGKGKDQP
                     EKAIPVLRKESDYLANYFNGKILGEQIVRSHINHVIIRTAIIYGYDLDGQPDRRMKIL
                     LDCYQAGQKYPRTANLFTSVIHVCDLADAVIEAAESGFKGTINISAEKPVSYYDLNRY
                     LAALLGINDNFITADYKPVPTYETLDNTLRKRLLKTPIRELQF"
BASE COUNT          753 a          707 c          798 g          687 t
ORIGIN      
        1 cagtggaaac gctttcggtg cgggtggaca agcttgagag agataccgcc gagctgttcg
       61 aaaaaaccaa taccgccgcc gtatcgcttg cagccgtcaa cgagaagctc aacagcctgc
      121 tcgtgacgct cggggaagtc aagcaggccg tcagcaatct tcgggatatt cccgcaaagc
      181 ggctggagaa tattcttaaa gcgctggcga ctgccgtatt atcaggaata accgggtata
      241 ttctcgcacg gttcaggtag agtgcagaat gaaaaccatg tttgaaagga atgttgaaga
      301 catgaatctg aaaattgact gggagagaaa actgacttcc cgtaaattct ggacggcgct
      361 gtgtggtttt acggccgctc tgctgacagc gtttcatgtt ccagcaggga cggccgctca
      421 ggtcacgtcg attattatgg cgggagcgtc gctgattgca tacattcttt ccgaagggct
      481 tgccgacgcg gcagcctcct ctgcccaaaa cggtgcgacc aatgtgcccg accctctgcc
      541 cggcgccgaa caaaagtaag taagtacaac acataaaaag aatcccgtgg gtcagtcccg
      601 cgggattttg tattttgcct tcaattatgc taaaataatg cagacgaaaa gccgcccggt
      661 accggacggc ctatggggcg gaaacaggac ccaatgaaag gcgcgtggtt ttgatgatgc
      721 agtatttaat tctgggagcc tgcctgataa caactgttct ttcctttgtg acgatgatgg
      781 cggcattctc cctcaaaaaa aatcagggcg gaaatatgga agattatcgc cgaatggaaa
      841 gtctgaaacg ggaaatcctt gacgagatgc gttccaaccg gcaggagacc tcgcagtcca
      901 tccaaagctc gatccgaagc atgagcgaac tgcttgtcaa aacacagacc cagacgatga
      961 gcattcagga tacaaggctc gcggagctca accgccagtt gaccctgcgg caggatgctt
     1021 tccaaaagac gatgggcgaa accctggggc gagtggataa aagaatgtct gagttggcgg
     1081 gccagaccga gattaggctt gagacgatcc ggacgacaat ggaaaccaaa cttacctcca
     1141 tgcaggagga caacacgaaa aaactcgatc agatgcgcgc gactgtcgat gaaaaactcg
     1201 aaaagaccct ccaggacagg atcggccagt ctttccgtct ggtcagcgag aggcttgagc
     1261 aggtttacaa ggggctcgga gagatgcaga gccttgccac gggcgttggg gatctgaaaa
     1321 aagtactctc caacgtcaaa acccgcggag tgctcggcga agtacagctt ggcgcgatct
     1381 ttgagcagat tttatcgcct gaacaatatt gccgcaacat agccaccaaa cgcggcagca
     1441 cctgcgttgt ggagtatgcg gttaagcttc cgggggacgg gagcggagag gttttgctgc
     1501 ctgtggacgc gaaatttccg gccgacgctt acgcgcagct gaccgatgct tacgacggcg
     1561 gagacccggc gttgattcag gcggccggtt cgatgctgga gcgccggatc aagctctttg
     1621 ccaaagacat tcgggataaa tacatagatc ctcccgcaac caccgaattc ggcgtgatgt
     1681 ttctgccgtt tgaggggctg tatgccgagg tggtgcgccg cgggctgctg gagacgctgc
     1741 agcgggacta taaggtcagc atagcgggcc cgacaaccat ggcggccctt ctgaacagcc
     1801 tgcagatggg ttttcggacg ctggccattc agaagcggtc gggagaagtc tggcaggtgc
     1861 tcggcgcggt caaaacagag tttgaaaaat tcggcggcgt ccttcaggcg gcgcagcaga
     1921 agctggaaca ggccaattca gagctggata agctcgtagg cgtgcgcacc cgccagattc
     1981 agagaaagct ccagtcggtt tccgcgcttc ccgagcagga aagcgagctt ctcatcgatt
     2041 cggattcctc agccgccgtg cgggagagcg ccattttttc cgctgattcc agcggcggga
     2101 atgatcagga aaatcaagag gtgtaaaagc atctttattt atatgaaaac tgacgaagtt
     2161 aaaactgtaa ctcgcgaatc ggtgttttca gtaacctttt tctcaatgta ttatcaagag
     2221 tttcataagt cggaacgggt ttatagtcgg cagtaataaa gttatcattg atgccgagca
     2281 aagctgccag atatcgattc agatcataat agctgacggg tttctctgca gaaatgttta
     2341 tcgtaccctt aaagccgctt tcagctgctt caataactgc gtcggccagg tcgcaaacat
     2401 ggataacact tgtaaagaga ttcgcggttc tggggtattt ctgaccggct tgataacaat
     2461 caagcaatat tttcattcgg cgatccggct gaccgtcgag gtcataccca tagataatcg
     2521 cggtgcgtat gatcacgtga tttatatgac ttcttacaat ctgttcccct agaatcttac
     2581 cgttaaaata atttgcaaga taatccgact cttttcgcag aaccgggatg gctttttcgg
     2641 gttgatcttt cccttttccc agttttgacg aaatatagac aaaccgggtt gtttccggaa
     2701 tcagctgaat cagcttctgc aagccggttt gtgataaagt aagctcattt gttcggtcac
     2761 aaacggccca aatgattaca tcaggttttt gaagatatat tttttgaata tccgtaggat
     2821 ttgtaaggtt tacccggatc aagcccggcg tattatgaga attgcaggtg ccgcttacat
     2881 ttgccatctt ttccgagagc tttttgtaga tggtttttcc caaatatccg cttgctccga
     2941 tcacc
//
LOCUS       DATALY010000123        19640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_718166,
            whole genome shotgun sequence.
ACCESSION   DATALY010000123 DATALY010000000
VERSION     DATALY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19640
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718166"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VHR42_03655"
     CDS             <1..420
                     /locus_tag="VHR42_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX3026307.1"
                     /translation="ELEKENLGKFQAAVRKIEQQYEEKIREQYLKNLETAFALAEKIL
                     DIEINRNDEAFLGLYKKAAAHIGKTESVQLKVGPRGYEVSSRNREELMKCISGLKNLE
                     LEESGEEGTCVIQSPMGEADASIKTQLNRAREIIQAS"
     gene            508..1809
                     /gene="fliI"
                     /locus_tag="VHR42_03660"
     CDS             508..1809
                     /gene="fliI"
                     /locus_tag="VHR42_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX3026308.1"
                     /translation="MAHERLLKIIDQADLTVCRGSVDRVVGLTVESVGPPVKIGEICN
                     VRTTQGKSVKAEVVGFRDKRMLLMPFDNLSGIGPGSVVESTGMPFAIPVGNGLIGRII
                     DGTGEPIDDKGPIKADNFYVVDGNPSNPLFRPRINKPISLGVRALDGLLTCGRGQRIG
                     IFAGSGVGKSTLLGMIARYAQADVNVIGLVGERGREVRDFIEKDLKQDGMSRSVLVVA
                     TSDRPAMVRLKCALVATTIAEYFRDQGKSVMLMMDSLTRFAMAQREIGLATGEPPVSR
                     GYTPSIFSIMPKLLERSGNFETGSITGIYTVLVEGDDMNEPISDTVRGILDGHIVLSR
                     ALAMKNHYPAINVLGSVSRVMPDIVGSDHLEVANQMRNLMATYANYADLIGIGAYKTG
                     SNPEVDRAIQYHEPINAFLKQTVDGSCDLGQTIELMKKTLS"
     gene            1849..2301
                     /gene="fliJ"
                     /locus_tag="VHR42_03665"
     CDS             1849..2301
                     /gene="fliJ"
                     /locus_tag="VHR42_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX3026309.1"
                     /translation="MKQFKFRLQQVLEFKRHLEMEQKDQLAVEKIKLDNFLAEETQLR
                     SKFQFWAKRYIEFSYKGISPAQAAEINGFLSELSRMIDQCIKKIQDQEARVETVRQQL
                     VEKMKDRKTLESLADKQSSRFLQEEMLKEEKSVEEIIVGRYHQNAPAF"
     gene            2395..3993
                     /locus_tag="VHR42_03670"
     CDS             2395..3993
                     /locus_tag="VHR42_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX3026310.1"
                     /translation="MQIEQITAQTGVKLQKTAKTLSSSASGGKFAEILSGEEDKQEDK
                     SDQTTPADGTVALIMMNPGLLMPSQTTSDLTAGPGISTAADGTEKDTSADLSGQADQK
                     TAATVTSAPPSVLTLGQTGITSVAAGVTLMPGAAQTQNPQAPASTETSPKRGTGTEST
                     ALPTEVPQQAGTFIQSKSPNTSAVTELPSESEPISQSVDGTEPKAFQSILTDTSAKVS
                     QAVTSTAAQNASPADTLAGQAVKDVKVSPLQSEMPQVKSDSAANMAQSNRISDGTREL
                     PQTDSGTQENTAKTDRMSQTAGASILRETPEQAVGTMKDSTQTSDISIAALGTKSAPD
                     SAASSAAKTTTGGKEDSTSQGQNGTTGGQNQDQSSGILAQTTVSSATPFELNVGDKVK
                     TEAASVANQLSRAVDDAVQSDRSEIRFHLSPEDLGGISIKIVSQSGSLSLQITAENHH
                     TGSLIASNMGELNKAIADRGINMNKAEVMLSSDGGYQSMSGSQERNLQQENGKMPVWV
                     PAAKQQEQDSAVEDNTTAGITILA"
     gene            4008..4502
                     /locus_tag="VHR42_03675"
     CDS             4008..4502
                     /locus_tag="VHR42_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX3026311.1"
                     /translation="MDISSITSAYDTTAASSSTTSSSSSSNSLGMSDFIQILAAELQY
                     QDPTQSTDNSQYMSQMAQFSVLEQIQSLSKSFNYSLSSQMVGKSVLYNTVVYDKSTAQ
                     YSTVTASGKVDAVDLSSDTPTCFINGAWVDLSSIKTVYDSTTTAPTTDTTDKTNSDNT
                     SKTT"
     gene            4530..4928
                     /locus_tag="VHR42_03680"
     CDS             4530..4928
                     /locus_tag="VHR42_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HEX3026312.1"
                     /translation="MPDLRLANSVFTPPLSAGVQTNASGRSTRVDDNSESFSDIFQRE
                     ISQNEGVKFSKHAQSRLESRNITLGQEDLTRLSQAVDQASQKGIEDSLMIMGNLAFIV
                     SVPQKTVVTAMPVNEAGGNVFTNIDGAVIL"
     gene            5010..6554
                     /locus_tag="VHR42_03685"
     CDS             5010..6554
                     /locus_tag="VHR42_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX3026313.1"
                     /translation="MMRSLTSAVTGLRAQQTAMDVIGNNIANVNTAGFKSSQTTFSDL
                     FYQTIGTGTDNIDPSQVGYGTQVAGVSKNMDSTGATQTDNPYDLYIDGEGYFAVNTRS
                     DGTGETYYTRLGKFKINKDGYMVDSNGNYVMSAKKTDSGKVVAGSPVCLIGTTLHADN
                     GQGILLDKEQYDKITDISVKQDGSISVSIGDIVGTLSFDKATTPTYLNASFKDADGNL
                     QTVTASNYNTVCDGMGVDGSGNVYATTTGGSPSTTAHSILYQPNTSVVGDTLYASDGT
                     TLTITNASALSGVTLNADGTVTATMGGKTGVIHDSKGALSMIGKTFIAADGTATAVTS
                     SNLAALSGLSVNAAGTMSVSLSGVSGSVSDGPVSVIGLNFHGADGTTTPITTTNSANL
                     SNIKMNADGSFTGTLNGVTGTFQNDSYTSMEIGVATFVNSSGLSEYGNSYFTSTASSG
                     DANYTIAGDENKTIIRNGRLEMSNVDLASEFTNMIVTQRGYQANSRVITVSDSMLEEL
                     VNLKRS"
     gene            6607..6900
                     /locus_tag="VHR42_03690"
     CDS             6607..6900
                     /locus_tag="VHR42_03690"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEX3026314.1"
                     /translation="MIKVTKLNCEVFYINPFEIEFIEETPDTVISLRSGKKVLVSEKA
                     SQVIDEMIEFYRQTNLKPVIRNPEDGKEAAEEIQPAETEDEAEDEAEGEEGEF"
     gene            6951..7700
                     /locus_tag="VHR42_03695"
     CDS             6951..7700
                     /locus_tag="VHR42_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3026315.1"
                     /translation="MSFIIGVALSAVLIVITVMRGANPALLLDYKALLFVVGGTVGSI
                     LITFPFSDLRKEMATIAKLLAPQQHPQKLITRISELAQLARKEGVLSLDGKSKSVDDA
                     ILSKGLNLISVSADHEIIRNILEKESDFIGRQEKSAQDFFERLSVLTPGIGMVGTLLE
                     IVQMLYRYTDPKKLAPEIAASLLPVVYGALLSYLILMPLASRLKVGANRRKMLRELAI
                     EGVLALQAGEPPYIVEERLGVYTRAADRKKL"
     gene            7749..8789
                     /gene="fliM"
                     /locus_tag="VHR42_03700"
     CDS             7749..8789
                     /gene="fliM"
                     /locus_tag="VHR42_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX3026316.1"
                     /translation="MSEVLSQNQIDDLLSALKSGDITDLDSGASEKKARPYDFKIPKK
                     FNKEQLKTLSIIYENYGRVLSSYLSGSLRTYCQVEVLSIEEQRYFEYSNALSENIMMG
                     IMEMQPLQGTAMITIGQTVAFAIIDRLLGGQGQAYEIDREYTDIETALMERVVRDLCT
                     LMKDAWSNVREITPKFIRLDTNSRQTQLVSPNETVVIIAMNIKIKDVEGNISFCMPYV
                     ILEPVLEHLNTKYWFTERKISEEDRRHSKESLLRKLKRVPIEMKVVLGSSQVTLNEIM
                     GLQAGDVIRLDQSQHENAVVMSNTSKWFTGQLGVLNNHIALKVNQVLLEGGDTDGSGT
                     KLNENKSNQGTN"
     gene            8737..9918
                     /gene="fliY"
                     /locus_tag="VHR42_03705"
     CDS             8737..9918
                     /gene="fliY"
                     /locus_tag="VHR42_03705"
                     /inference="COORDINATES: protein motif:HMM:NF005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HEX3026317.1"
                     /translation="MDQEQSSTKINLTKEQIDILGEVSNISMGSAATALSNIMGKKVT
                     ITSPHLEIGSVEVVDKIQRIPSIGVVISYTEGVVGKDILIIRKDDAIEIVKVLMGGEV
                     SSEDFDEIYLSAISEVMNQMMGSSATALSNFIGKTVNISPPTAFVLTDENKTEKLKFI
                     YDNVDNIVLVRFLFNVEDVLESDIYMIMDQEFSSKLVSAMMKNIENSAPAQPPKAEIP
                     PVPETPAIPEMPAIPEMPDVPVMPQAGQPSVPPAIPQPPEDMMQNTGMLEAAASAVRE
                     PIGMASPPIPSRTVPQTPVRPVTLSNFDSNQSGLNVNEVSNFNLIQDVPLELSVEVGR
                     AKRLVKEVIDISVGSIIELDKQAGDPVDVIVNGQLIAHGEVVVIDESFGVRITDIVEK
                     K"
     gene            9946..10311
                     /locus_tag="VHR42_03710"
     CDS             9946..10311
                     /locus_tag="VHR42_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3026318.1"
                     /translation="MAKKIMIVDDAAFMRMMLKNILTKGGYEICEEAQDGLQAVEKFV
                     SAEPDLTIMDITMPNMSGIDALKKIKELNANAKIIMCSAMGQEAMVVDAIKAGAMDFI
                     VKPFQGDRLLAAIERVMPN"
     gene            10410..10814
                     /locus_tag="VHR42_03715"
     CDS             10410..10814
                     /locus_tag="VHR42_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX3026319.1"
                     /translation="MLGYLLTLLVSLVIVVVFFFLAWVFTRLVASNGSFNGKGQFITI
                     LERFPVTKDSSILLVKTFDKVMLIGVTPHGMTTLNEYDAEGIDIQNTEFKKQSFSEIM
                     SSAFDSAFPDGKVRKTVDRMLKKGKGGKKGEQ"
     gene            10912..11568
                     /gene="fliP"
                     /locus_tag="VHR42_03720"
     CDS             10912..11568
                     /gene="fliP"
                     /locus_tag="VHR42_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828684.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX3026320.1"
                     /translation="MSLNGAQTSQGTANSLQILFMLTIISLAPSILIMMTSFTRIIIV
                     LSFLRNALGLQTTPPNQVLVGIALFLTMFIMAPVGTQIYDTAYKPLTENKITTNQAYS
                     NAMKPVRDFMFRQTEKSSLKSFMNMAKMKPVSALNQIPSRVLIPAFVISELKRAFMMG
                     FIIYLPFLIIDLIVSSTLMSMGMMMLPPMMISLPLKLALFVLVNGWGLTVESLVRSFK
                     "
     gene            11624..11887
                     /gene="fliQ"
                     /locus_tag="VHR42_03725"
     CDS             11624..11887
                     /gene="fliQ"
                     /locus_tag="VHR42_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX3026321.1"
                     /translation="MDEGQIIDIAQRAIYVASITAIPILIACLAVGIVISIFQAATQI
                     HEQTLVFIPKIVVVVILLAVAGSWISGQLAEFTREAFGVIANL"
     gene            11951..12724
                     /gene="fliR"
                     /locus_tag="VHR42_03730"
     CDS             11951..12724
                     /gene="fliR"
                     /locus_tag="VHR42_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX3026322.1"
                     /translation="MTISAMSLNLFLLVFMRISGAVIFNPLLGRNNVPVTLRGALSLI
                     IAFVVFSTVNSPSTVAGGVVQLIVQCVFELFIGFIIGTVVNIFFAVVLIAGEIIDTQM
                     GLSMARYYDPNSGVSMPILGSYFNALMVLVFFASNAHLSLLTLTADSFKLIPPGTVGF
                     APKAAQYMVMLGKDMMEFGLRMAIPVLAVEMVCIVAIGLLMRAVPQINIFSVGIQIQA
                     IVGIIIVAISVPVIVPMCSRLSTLIVEKSVVLVRMLVNS"
     gene            12738..13796
                     /gene="flhB"
                     /locus_tag="VHR42_03735"
     CDS             12738..13796
                     /gene="flhB"
                     /locus_tag="VHR42_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEX3026323.1"
                     /translation="MPGDKSEKATSKKREDERKKGHVHQSKDLVSAISLISLFACLKY
                     MAPGFLNKMISGMTLLFEGMSSVITGPGDINSRLWVVVTVSASVILPFAAIVILVSVV
                     TTLAQTRLLFTPGQLKPSFDRMNPIKGIQRLFSLNGLMEVFKSLLKITIIGVMIYFEI
                     RPKIGQVLLLFDSDLGRSISWVGEIVLDVGFKVAIAMLVLGVIDYFYQWWSFERQIMM
                     SKQEIKDEFKQSEGSPEIKGRIRRMQRKMAQTRMMQAVPEADVVIRNPTHYAIALKYE
                     EKSGSAPLVIAKGKDYIALKIVEIAIQNNVSVTENRPLAQALFKTVEIGQEIPSEFYK
                     AVAEVLAYIYKLKRAGRQ"
     gene            13828..15885
                     /gene="flhA"
                     /locus_tag="VHR42_03740"
     CDS             13828..15885
                     /gene="flhA"
                     /locus_tag="VHR42_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX3026324.1"
                     /translation="MLEKIKGSIVSIAVICVIVLIILPLPAFLLDFLLIVNLSLAMLI
                     LFITMYTKNALQFSIFPSLLLITTLFRLSLEISATRLILTNNGDAGQVIQTFGNFVIK
                     GNILVGLVIFIIIVVVNFIVITKGSERVAEVAARFTLDAMPGKQMAIDADLNSGLITE
                     MDAKKRRSDIQREADFYGSMDGASKFVKGDAIVAIVIVIVNIAGGIIAGLTSGEDISK
                     VMSSFTLATVGEGLMAQLPALLISTATGIIVTRAASQGNMSEELSAQLFSQPLLLRTT
                     GIVLLCMLLLPGFPKPVLLLIGGMFLFLSFQVKGKKPGEENNGATVQEPKRLSAAGSE
                     LERFKNPDNVYQLLEIETIEMEFGYSIIPLVDETQGGTFVDKVVMFRRQYAIETGIVI
                     PSVRMRDNIQLSNNEYIIKIRGETVAQGEIFPNQQMIMNPVSDDFIIDGIDTVEPAFG
                     LKARWVPQEKREEAEMAGYTVIDPAAVMMTHLSEVIKKHIYELIGRKEINALLDMLKR
                     SNQSLVDEVVPAKLSVGDLQKVIQNLLRENIPIRDLVTILETLADHAPKIKDTDLLTE
                     YVRQSLKRSISRKFAPEGTLNVLTVDPQIEKMITSAVRQTDGGTYLALDPDKAQTVIN
                     SLSEQVAQFEKAGIPPVVLASPVVRMYFKKMTEQVMPDLTVVSYNELENDVRIQALGA
                     VAA"
     gene            15924..16682
                     /locus_tag="VHR42_03745"
     CDS             15924..16682
                     /locus_tag="VHR42_03745"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX3026325.1"
                     /translation="MTTLVDETTVSKLWRRFKQTDDIGLRNDLVLSYAGLVKSIVRRI
                     ASICGNYADIEDLTSYGMIGLIKAVEKFDLEKGVTFETFATYRVRGEIIDFMRRNDWV
                     PRGVRKRAWDIENTSNIMTNELGRVPSDDELAKKLGLKKDNLLQVLSEIERFNLVSFE
                     EIIQDTVKIDNQFADFDTPEGFLQEDELLDVLAKTLDSLPEREKLVITLYYYEELTLK
                     EISSIIGVSESRISQLHSRAINRMKKTLQSYVNS"
     gene            16702..17430
                     /locus_tag="VHR42_03750"
     CDS             16702..17430
                     /locus_tag="VHR42_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HEX3026326.1"
                     /translation="MIRGIFTSATGMIMQQKKLDVIGNNIANDQTSGFKSDEVSLRSF
                     DQEMIQRMSEDVQVGSMTYGVTSDGIKTDFSNGNYSQTNSATDIAIVGNGYFAVQDGN
                     NGVKYTRDGGFTVDSAGYLALPSGERLLGTNGQPLKVSGDQFSLSSDGVVRNKSGAVG
                     QIAVYQPTGQIVKRGDGFMQMTGASQIKGTLRQGFLEGSNVDIVTQMSQMMVATRSYQ
                     SNQQSFKTADQTLEKLVNQVGSMR"
     gene            17455..18159
                     /locus_tag="VHR42_03755"
     CDS             17455..18159
                     /locus_tag="VHR42_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HEX3026327.1"
                     /translation="MIRAFYSGTAGIKAQQEVVDIVSNNIANVNTEGFKSKSGQFGDL
                     LYTSMEEKTGRSKTVLAGSGGAVAEVKNNMSDGGCTQTGRLLDYKIDGSGFFRVQDGS
                     GNNYYTRNGAFSIANENGGMYLVNSAGMSVLDAKGNKIKVTGTGAVQAQPGVYDFPNE
                     SGLHNQGNNLFSASNTSGGAYAVDTKVMTGYLESSNVDLAQEMTRLIMSQRDYQLSAR
                     VVSTADQLANMANEMS"
     gene            18248..18568
                     /locus_tag="VHR42_03760"
     CDS             18248..18568
                     /locus_tag="VHR42_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX3026328.1"
                     /translation="MKIDGINIQKIYESFSKNAESAAEEACATKQDQGADPKDRVEIS
                     SKASDMSFARKTAQKQIGVESEQDREARIFQIKNQIESGQYSISSRDVAASIVKGRNF
                     DKNV"
     gene            18581..19054
                     /locus_tag="VHR42_03765"
     CDS             18581..19054
                     /locus_tag="VHR42_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEX3026329.1"
                     /translation="MPSADWETELVNLLEKERKILTRFMDIAHQKTEALTGGNISEID
                     KFVNIEQPLVVQMSALESGRISLLGKKNFQGLTLSQLVEKSGQKKSELLELFDSMNSL
                     VSELRAANDLNSNITKSRIEFYNFLSGKKQKKNLYENSGRVKGVSEKTGLIDRKI"
     gene            19127..>19640
                     /locus_tag="VHR42_03770"
     CDS             19127..>19640
                     /locus_tag="VHR42_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEX3026330.1"
                     /translation="MPSTFAGLESARSGLTASQYGLDVTSQNVANAGTTGYSRQTLNM
                     TSAGPYVGAYRTLPAGTLLGQGVNIGGTDQVRDEFLDTRYRSQNATYNEWKNLSDGLT
                     SVEDVVNEFTSDGSGTIVGISGQLKDLVDDLQSYSNTPDDANLPTTVKSDFQTLCQSI
                     RTAYSSLESLS"
BASE COUNT         5452 a         4285 c         5066 g         4837 t
ORIGIN      
        1 gaacttgaaa aagaaaattt ggggaagttt caggcggctg tccgtaaaat tgagcagcaa
       61 tatgaagaga aaattcgaga acaatacctt aaaaatttag aaacggcatt cgctcttgcg
      121 gaaaaaattc ttgatattga aatcaacagg aatgatgaag cgtttcttgg attatataaa
      181 aaagcggcgg ctcacattgg aaaaaccgaa agcgttcagc ttaaggttgg gcccagaggt
      241 tatgaagttt ccagccgcaa cagggaagaa ctgatgaaat gcatcagtgg gctgaaaaac
      301 cttgaattag aagaaagcgg tgaggaagga acttgtgtga ttcaatcgcc aatgggtgaa
      361 gccgatgcaa gtataaaaac ccagctcaat cgggcaagag agattataca ggcatcctga
      421 ttccgttcct tattccgttc ctttagaggg cattcggcgg tttagcagcg cgtcctattt
      481 ctcaggcctt gtcggctgga ggtgcagatg gcacacgaaa ggttactcaa gatcattgat
      541 caggctgatt tgacggtttg caggggaagt gtcgataggg ttgtaggctt gacggttgag
      601 tcagttgggc cgcctgttaa aatcggagag atttgcaatg tcaggaccac gcagggcaaa
      661 agtgtcaaag cagaggtagt gggttttcgc gacaagcgaa tgctgctgat gccctttgat
      721 aacttgtcgg gtatcgggcc tggaagtgtt gtggaatcga ctggtatgcc gtttgctatc
      781 ccggtgggca acggcctgat tggcagaatt atcgacggaa ccggggaacc aattgacgat
      841 aagggcccaa tcaaagccga taatttctat gttgtagacg ggaacccgtc caaccctctt
      901 ttcaggccga gaatcaacaa gccgatttcc ctgggggttc gggcattaga cggactgcta
      961 acctgcggaa gaggccagag aatcggcatt tttgccggaa gcggagtcgg aaaatcgact
     1021 cttttaggaa tgattgcaag atatgcccag gccgatgtca atgtcatcgg gcttgtgggc
     1081 gaacgcggca gggaagttcg cgattttatt gaaaaagatc tcaagcagga cggcatgagc
     1141 cgttccgttc tggtggtggc cacctcagac aggcccgcga tggttcggct gaaatgtgcc
     1201 cttgttgcaa cgacgatcgc agagtatttc agagatcagg gtaagtcggt tatgctgatg
     1261 atggattcgc tgacacgttt tgccatggcg cagcgtgaaa taggcctggc gaccggcgaa
     1321 ccgccggttt ccagaggata cactccttcc atattttcga ttatgccaaa gctgcttgag
     1381 cgatcgggca attttgagac cggatccatt accggaattt atacggttct cgtggaaggg
     1441 gacgatatga atgagccaat ttccgacact gtcaggggga ttcttgacgg gcatattgta
     1501 ttgagccgtg cgctcgcgat gaaaaaccat tatccggcta tcaatgttct gggaagtgtc
     1561 agccgcgtta tgccggatat tgtgggcagc gatcatttgg aggttgccaa tcagatgcga
     1621 aatttaatgg ctacttatgc gaattatgcc gatctaatcg gcattggtgc ttacaaaacg
     1681 ggaagcaacc cggaagtgga cagggcgatt caataccacg aaccgatcaa tgcattttta
     1741 aaacagacgg tggatggcag ttgcgatctg ggccaaacga tcgagctaat gaaaaaaacg
     1801 ctttcttagt tttgacagaa tcaaatcagc aagatgggaa gcgctcggat gaaacagttt
     1861 aaattcagac ttcagcaggt gcttgagttc aagcgtcatc ttgaaatgga acagaaggac
     1921 cagcttgctg tggaaaaaat aaaattggat aattttctgg ccgaggaaac gcagctgaga
     1981 agcaagtttc agttttgggc caaacgctat atagaatttt cctataaagg aataagtccc
     2041 gcgcaggctg cggaaataaa cggttttctt tccgaactaa gccggatgat cgatcagtgc
     2101 atcaaaaaaa ttcaggatca ggaagctcgg gtggaaacgg tcaggcagca gcttgtggag
     2161 aaaatgaaag atcgcaagac cctggaatca cttgccgata aacagtcatc acggtttttg
     2221 caggaggaaa tgctcaagga agagaagtca gtggaagaaa ttatcgtcgg cagatatcat
     2281 cagaatgcgc cggctttttg atttcggcca acgatttctc cggtattctt atcccgatca
     2341 attgacgaaa cctgtcattt tatagaggtt gaactattga aaggaggtga aaaaatgcag
     2401 atcgagcaaa ttacggctca aacgggagtg aaactccaaa aaacggcgaa aaccttatcc
     2461 tcttcagcga gcggaggaaa atttgcggag attctttccg gcgaagaaga caaacaggaa
     2521 gataagtcag atcagaccac ccctgcggat ggaaccgttg cgctaatcat gatgaatccc
     2581 ggattgctga tgccatcgca aacaacttcg gatcttacgg cagggccggg catttcaact
     2641 gccgctgacg gaactgagaa agacacatcg gctgatctgt ccgggcaggc cgatcaaaag
     2701 acagccgcca cggtaacctc agcgccgcca tccgttctca ctcttgggca gacgggaatt
     2761 acaagtgtgg ctgccggggt aaccttgatg cccggtgcgg cacagacaca gaatccacag
     2821 gcaccggctt ccaccgaaac gtcaccgaag cgcgggaccg gaacagaaag tacggcgctt
     2881 cctactgaag ttccacagca ggcgggaact ttcatacaaa gcaaaagccc aaacacgtcg
     2941 gcagttaccg aattgccttc ggagtctgag cccatttccc aaagtgtcga tggaaccgaa
     3001 ccgaaagcat ttcaatctat tctgacggat acttccgcaa aagtttcgca agcggttaca
     3061 tccacagcgg cgcaaaatgc atcgcctgct gataccttgg ccgggcaggc tgtgaaggac
     3121 gtgaaagttt ctccgctgca gagtgaaatg ccacaggtaa aaagtgattc cgcagcaaac
     3181 atggcgcaaa gcaatcgtat ttctgacggg acgagagaac tccctcaaac cgattccggt
     3241 acacaggaaa ataccgctaa aacggatcgt atgagccaga cggcgggtgc ctcaattctt
     3301 cgtgaaacgc cggagcaagc cgttgggacg atgaaggaca gtacgcagac ctcagacatc
     3361 tcaatagcgg cattaggaac aaaatccgcg cccgattccg cagcatcttc cgcagcgaaa
     3421 acaacaacgg gcgggaagga agattcaact tcccagggcc agaatgggac gacgggagga
     3481 caaaatcaag accagagttc cggaatattg gcccagacga ctgtctcaag tgcaacgccc
     3541 tttgaactga atgttggtga caaggtcaaa acagaggccg cgagtgtggc aaatcagctt
     3601 tccagggcgg ttgatgatgc agttcagtcc gacagaagtg aaattcgatt tcatctcagc
     3661 ccggaggatt taggcggaat cagcattaaa attgtgtccc agagcgggtc gctttccctt
     3721 cagatcacag ctgaaaatca tcacaccggt tcactgattg catccaatat gggagaactc
     3781 aacaaggcga ttgccgaccg tggaatcaat atgaacaagg cggaggtaat gctttcgtcc
     3841 gacggcgggt atcaatccat gtccggctcg caggaaagaa acctgcagca ggaaaacgga
     3901 aaaatgccgg tatgggttcc ggcagcgaaa cagcaggagc aagattcagc agtagaagat
     3961 aacacaacgg cgggaattac aattcttgcc tgagacagga ggttcccatg gatatttcgt
     4021 ccatcacgtc cgcatacgat acgacggcag cgagcagcag tacgacgagt tcgagcagta
     4081 gcagcaacag cctcggtatg tcagatttca ttcagatttt agcggccgag cttcagtatc
     4141 aggatccgac gcaatccaca gacaacagcc agtatatgtc tcaaatggct caattcagcg
     4201 tacttgagca gattcaaagc ctttccaagt cgtttaatta ttctctttct tcccagatgg
     4261 ttgggaaatc ggtgctttat aacacggtcg tgtatgacaa atctaccgcc cagtattcaa
     4321 ctgtaaccgc atcgggaaaa gtggatgctg tggacttaag ttcggatacc ccaacatgtt
     4381 ttattaacgg agcatgggtc gatctttcca gtattaagac ggtatatgac agcacgacta
     4441 cggcaccaac aacagacaca acagacaaaa cgaattccga taatacgagc aaaacaacct
     4501 aatcagcgct caaatgcgag gtgatcagtt tgccagattt aagactggcc aattccgtat
     4561 ttacgccgcc tctgtctgcc ggtgtccaaa caaacgcaag cggccggagc acccgggttg
     4621 acgataattc tgaaagcttt tccgatatct ttcagcgcga aatcagccag aatgagggcg
     4681 tgaaattttc caagcatgca cagtcaagac tcgagtcaag aaatattact ctcggtcagg
     4741 aagatctgac ccggcttagc caggcggtgg atcaggcatc acaaaaaggc atcgaagatt
     4801 ccctgatgat tatgggtaat cttgcattca tcgtcagcgt ccctcaaaaa acggtagtga
     4861 ccgccatgcc ggttaacgag gcgggaggaa atgtatttac gaatattgac ggagctgtga
     4921 ttttataacc ggtcctcttg aggaggttat cgggttttga atgattgata aacccgcacg
     4981 gctcaaaata cgaggaggat gtattcatta tgatgagatc attgacttca gcagttacgg
     5041 gtcttcgtgc ccaacagacc gcgatggatg tcataggtaa caacatcgca aacgttaata
     5101 cggcgggatt caaatccagc cagacgacct tcagcgatct gttttatcag acgattggca
     5161 ccggtaccga taatattgat ccttcccagg ttggttatgg cactcaggtg gccggagtca
     5221 gcaaaaacat ggatagcaca ggcgcaacgc aaaccgacaa cccttatgat ctttatattg
     5281 acggcgaagg atattttgcc gttaacaccc gttccgacgg gacgggcgaa acctattaca
     5341 caagacttgg caaatttaaa atcaacaaag atggctacat ggttgacagc aatggcaatt
     5401 atgtaatgag tgccaagaag acggacagcg gcaaagtggt cgcaggttcc ccggtctgtc
     5461 ttattggcac aactctgcat gcggataatg gccagggcat tctgcttgat aaggaacaat
     5521 atgacaagat aaccgatatt tcagtcaagc aggacggctc cattagtgtg agcatcgggg
     5581 atattgtcgg aacgctttct tttgacaaag cgacaacacc cacctatctg aacgcatcct
     5641 ttaaagatgc cgacggcaac cttcagaccg tgaccgcttc caactataat acagtctgcg
     5701 acggtatggg agtcgacggc agcggaaacg tatacgcaac gaccaccggc ggttcgcctt
     5761 ctacgacggc tcattccatc ttatatcaac cgaatacatc ggtggtaggg gatacgctgt
     5821 atgccagcga cggtacgacc ctgacgatta caaatgcgtc ggccctttcg ggtgttacgc
     5881 tgaatgcgga cgggacggtt accgctacaa tgggcggaaa aacgggagtc attcatgact
     5941 caaaaggcgc tctctccatg attggaaaaa catttatagc ggccgacggc acagctacgg
     6001 cggttacttc ttccaacctt gccgctctct ccgggttaag cgtgaacgct gccggaacca
     6061 tgagcgtctc tctttccggc gtgagcggat cagtctcgga cggccctgtt tcggtgatcg
     6121 ggcttaattt ccacggcgcc gacggaacaa ccactccgat taccacgacc aacagtgcca
     6181 atctttccaa cattaaaatg aatgccgacg gatcttttac aggcacgctg aatggcgtga
     6241 caggaacgtt ccaaaacgat tcttatactt ccatggaaat cggggttgct acctttgtaa
     6301 actccagcgg cctttcggaa tatggtaaca gctattttac ttctaccgcc agttcgggcg
     6361 acgccaacta taccattgcc ggggatgaaa ataagaccat tatccgaaac ggcagactgg
     6421 aaatgtccaa cgtcgatctt gccagtgaat ttacgaacat gattgtaacc cagcgtggtt
     6481 atcaggccaa ctccagagtt atcaccgtat ccgactccat gctggaagag ctggttaatc
     6541 ttaagaggtc ataatgctga taagaaatgc cgggctgctt aaaagcagcc ggaatgaggg
     6601 tttattatga taaaagtgac caagctgaat tgcgaagttt tctatattaa cccttttgaa
     6661 attgaattta tcgaggagac tccggacact gtaatctctc tcagaagcgg gaagaaagtg
     6721 ctcgtatcgg agaaagcaag ccaggtaatt gacgagatga tcgaatttta ccgccagacc
     6781 aatcttaagc cagttatcag aaacccggaa gacgggaaag aggcggcgga agaaattcag
     6841 cctgccgaga ctgaggatga ggctgaggat gaggccgagg gcgaagaagg ggagttctga
     6901 gaaacccggc ccgcccgaaa acggacgaag ctaaggaggt tctttgaacg gtgtccttta
     6961 ttattggagt agcgttaagc gcagttttga tcgtgattac ggtaatgcgg ggggccaacc
     7021 ccgcactgct gcttgattac aaagccctgc tgtttgttgt cggcggcacc gtcggttcca
     7081 tactgataac attccctttt tcagacttac gcaaggaaat ggcaaccata gcgaaattac
     7141 ttgcacccca acagcacccg caaaagctga ttacccggat atccgagctt gcccaactgg
     7201 caagaaaaga aggggtcctg tcacttgacg gaaagagcaa atcggttgac gacgctattt
     7261 tatcaaaagg gctgaatttg atttcggttt ccgccgacca tgagattatt cggaacattc
     7321 ttgaaaagga atccgatttt atcggacggc aggagaaatc ggcccaggat ttttttgagc
     7381 gactgtcggt ccttactccc ggaattggta tggtgggaac actgctcgaa attgttcaga
     7441 tgctttaccg ttatacagac ccgaaaaaac ttgcaccgga aattgcagca tcgctgctcc
     7501 cggttgtcta tggcgcactg ctgagctacc tgattctgat gccgctcgca tccagactta
     7561 aagtgggtgc aaatcgccgg aaaatgctcc gggagctggc gatcgaagga gttcttgctt
     7621 tacaggcggg cgagccgccg tatatcgttg aggagaggct gggcgtttac acccgggccg
     7681 ccgatcgaaa aaagctctga ttcaggcgca ggagacaaaa accgaaacgg agcagatgga
     7741 ggttaaatat gtccgaagtt ctttcacaaa atcaaataga cgatttgcta agcgcgttaa
     7801 aaagcggcga tattacagat cttgattccg gagcgtcgga aaaaaaggcc cggccctacg
     7861 actttaaaat acccaaaaaa tttaataaag aacagttaaa aacgctcagc atcatctatg
     7921 aaaactacgg ccgcgtttta tcctcttatc tctctgggtc gctgcgaact tattgtcagg
     7981 tcgaggtgct gtctatcgaa gagcagcgtt acttcgagta ttccaacgct ctctcggaga
     8041 acatcatgat gggtattatg gaaatgcagc cgcttcaggg tacagcgatg attacgattg
     8101 gccagacggt tgcctttgcg atcattgaca gactgcttgg gggccaggga caggcatatg
     8161 aaatcgacag ggaatacacc gatatcgaaa ccgctcttat ggagagggtt gtacgggatt
     8221 tgtgtacgct tatgaaggac gcttggtcca atgtcagaga aattacgcca aagtttataa
     8281 ggcttgacac caattccaga cagactcagc ttgtatctcc aaatgaaact gtcgtgatta
     8341 tcgcgatgaa tattaaaatc aaggatgtcg aaggcaacat cagtttctgt atgccttatg
     8401 taattctgga gcctgttttg gaacatttga atacaaagta ctggtttacc gaacgaaaaa
     8461 tttcggaaga agaccgcagg cacagcaaag aatccctgtt aagaaagctc aaacgtgttc
     8521 cgattgagat gaaagtggta ctcggatcaa gccaggtaac gctcaatgaa atcatgggcc
     8581 tccaggccgg agatgtcatt cggcttgacc aatcccagca cgaaaacgca gtggtgatgt
     8641 caaacacaag caaatggttt acggggcagc ttggagtttt gaataatcat atcgcgctta
     8701 aagttaatca agtacttctg gagggtggag atacagatgg atcaggaaca aagctcaacg
     8761 aaaataaatc taaccaagga acaaattgat attctcggag aagtcagtaa tatcagcatg
     8821 ggctccgccg ccacagccct ttcgaatatc atggggaaaa aagttacaat tacatcgccg
     8881 catcttgaaa tcggctcagt ggaagttgtt gacaaaatac agagaatccc atccatcggg
     8941 gttgttatct cctatacaga aggagtcgtt ggaaaagata ttctgattat tcgaaaagac
     9001 gacgcgattg aaatcgtcaa ggtgctgatg ggcggagaag tttccagtga agattttgac
     9061 gagatctatc tcagtgcaat ttctgaagtg atgaatcaaa tgatgggttc atctgcaacg
     9121 gcattatcaa actttattgg gaagacggtg aatatttctc cccctaccgc ttttgtactg
     9181 acggatgaaa ataaaacaga aaagctcaaa tttatatatg ataatgttga caacatcgtt
     9241 ctggttcgtt ttctgtttaa cgtggaagac gttctggaaa gcgatattta tatgatcatg
     9301 gatcaggaat tttcgtcgaa gcttgtcagc gccatgatga aaaacattga gaattctgct
     9361 cctgcgcagc caccaaaagc cgaaataccg cccgtacctg agacaccggc tatacccgag
     9421 atgccggcta tacctgaaat gccggacgta ccggtgatgc ctcaggccgg ccaaccctcg
     9481 gttcctccgg ccattcctca gccgccggaa gatatgatgc agaataccgg tatgctggaa
     9541 gccgcggcga gcgcggtacg cgagccgatc gggatggcat cgcctccgat accttcccgt
     9601 actgtcccgc agactccggt cagacctgtg acgctatcca attttgatag taatcaatcg
     9661 ggtttaaacg ttaatgaggt ttccaatttc aatcttatcc aggatgtgcc tcttgagctt
     9721 tcggttgaag taggcagggc gaagaggctg gtaaaagagg ttatcgatat ttcagttggt
     9781 tccatcattg aattggataa gcaggcgggc gacccggttg atgttattgt aaacggccaa
     9841 cttatcgctc atggcgaagt ggttgttata gatgagagtt ttggagttcg cattacggat
     9901 atcgtagaaa aaaaatgaag ggaaatctaa cagggggttt atgacatggc aaaaaaaata
     9961 atgatagtag acgacgcagc ttttatgaga atgatgctaa agaacatact gacaaaaggc
    10021 ggatacgaaa tttgtgagga agcacaggat gggcttcagg cagttgaaaa attcgtatcg
    10081 gccgagcccg atctgaccat tatggatatc accatgccca atatgagtgg tattgacgca
    10141 ctgaaaaaaa tcaaagaact caatgcgaat gcaaaaatta ttatgtgctc tgcgatgggg
    10201 caggaagcta tggttgtgga tgccatcaag gctggggcta tggattttat tgtgaagccc
    10261 ttccagggtg acaggctgct ggcagctatt gaacgcgtca tgccgaatta aaggattttt
    10321 ccgctttctc cgggtgcgaa ttgcacactc tttctccaaa gaatgggcaa agatttgttt
    10381 tttcgaaata attagggggg aattcgatat tgctaggata tcttctgact ttgctggtaa
    10441 gccttgtcat cgtcgttgta ttctttttcc tggcgtgggt gtttacacgg ctggttgctt
    10501 ctaacggaag ctttaacggc aagggtcagt ttattaccat actcgagcgg tttcccgtta
    10561 ccaaagactc ctccatcctt cttgtcaaga cttttgacaa agtgatgctg attggggtaa
    10621 cccctcacgg gatgaccaca cttaacgaat acgacgcaga aggtatcgac attcaaaata
    10681 cggaatttaa aaaacaaagt ttttctgaaa taatgagctc ggcgtttgat tcggctttcc
    10741 ctgacggcaa agttcggaaa acggtagaca gaatgctgaa aaaagggaaa ggcggcaaaa
    10801 aaggtgagca gtaaaaaaat acggctgatc attaagctgt tagtttccat cgcggttttt
    10861 gcaggcgttt ttacagcgac ggcagccgcg gcggcgcagc cgggagtcac catttctctt
    10921 aacggagcgc aaacatctca gggcacggcc aattcgcttc agatcttgtt tatgctgacc
    10981 attatttcat tggcgccttc cattttaatc atgatgactt cttttacgag aattatcatc
    11041 gttctgtcgt ttttgagaaa cgcattaggg ttgcagacga cgccgccgaa ccaggtttta
    11101 gttggtattg cgcttttcct gacgatgttt attatggctc cggtgggcac tcagatttac
    11161 gatacggctt ataaacctct gaccgagaat aaaattacca ccaatcaggc ttattccaac
    11221 gcaatgaaac cagtgcgtga ttttatgttc cggcagacgg aaaaaagcag tttgaaatcg
    11281 tttatgaata tggcaaaaat gaagccggtc agcgcgctga accaaatacc cagccgcgtg
    11341 ctcattcctg cgtttgtcat cagtgagctg aaaagggcct ttatgatggg ttttatcatc
    11401 tatctgccgt ttttgatcat cgacctgatc gtctcgtcga ccttaatgag catgggcatg
    11461 atgatgctgc cgccgatgat gatctccctt ccgctgaaac ttgcgctttt cgtgctggtc
    11521 aacgggtggg gcctgaccgt ggaatccctt gtgagaagtt ttaaatgacg atgctgtttt
    11581 cggtccgtca tgctgcgaaa ggttggaggc tgaagtttaa gttatggatg aaggccagat
    11641 tattgatatc gcgcaaaggg caatctatgt cgcttcgatt accgcaatac cgattttaat
    11701 cgcttgtctt gcggttggta tagtcatttc gatttttcag gcagcaactc agattcacga
    11761 gcaaacactg gtgtttattc caaaaatcgt tgtggtcgta atccttctgg cggtcgctgg
    11821 aagctggatt tccggccaat tggccgagtt cacgcgggaa gcctttggag tcatcgccaa
    11881 tttgtagatt tctatcggcc ggtgtcagaa ttatcgaggc tgttttgatt ttgggaaatg
    11941 ggagattatt gtgacgatat ccgcgatgag tctgaatctg tttcttcttg tttttatgag
    12001 aataagcggg gctgtaattt ttaatcccct gctcggcaga aacaatgtgc cggtaacgtt
    12061 aagaggcgcg ttaagcctga ttatagcctt tgtggttttt tcaacggtca attcgccttc
    12121 cactgttgcg ggcggcgtgg tgcagctgat cgtgcaatgt gtttttgagc tcttcatcgg
    12181 ttttataatt ggtacggtcg tcaacatatt tttcgctgtg gttttaatag caggcgagat
    12241 tatagatact cagatgggtt tgtcgatggc gcgctattat gacccgaact ccggcgtttc
    12301 aatgccgatt ctcggcagct atttcaatgc tcttatggta ttggtgttct ttgccagcaa
    12361 cgcgcatctg tcgctgctca cgcttacggc ggattccttt aagctgatcc ctcccggaac
    12421 agtgggcttt gctccaaagg ccgcgcagta tatggttatg ctgggtaaag atatgatgga
    12481 attcgggttg cgtatggcga ttccggtatt agccgttgag atggtttgca ttgttgccat
    12541 tgggttgctg atgcgggccg ttccgcagat caatatcttt tccgtcggta ttcagattca
    12601 ggcgatcgtc ggcattatca tagtcgccat atccgtgccg gtgattgttc cgatgtgcag
    12661 ccggctttcc acgctgattg ttgaaaaatc agttgtgtta gtcagaatgc ttgtgaattc
    12721 ctgagggggg atcttggttg ccgggcgaca aaagcgaaaa agcgacatct aaaaaacgtg
    12781 aggacgaacg gaaaaaaggt cacgttcacc aaagtaagga tctggtaagt gccatttcgc
    12841 tcataagcct gtttgcctgt ttaaaatata tggcgcccgg gtttttgaat aaaatgatca
    12901 gcggcatgac gctgctgttt gaaggaatgt cctccgtgat cacagggccg ggcgatatca
    12961 atagcagact ttgggttgtt gtgactgttt cggcctcggt cattcttcct ttcgccgcca
    13021 tagtcatttt agtttcggtg gtcacgacat tagcccaaac cagattgctg tttacacccg
    13081 gtcagctcaa accatcgttt gaccggatga acccgatcaa gggtattcag aggcttttct
    13141 cactgaacgg gctgatggag gttttcaaat ctcttcttaa aattaccatc atcggggtca
    13201 tgatttattt tgaaatcaga ccgaaaatcg ggcaggtttt actactgttt gattccgatc
    13261 ttggccgttc aatttcctgg gtaggcgaaa ttgtgctgga tgtcggtttt aaagtcgcaa
    13321 tcgccatgtt ggttctcggc gtgatcgatt atttttatca gtggtggtcg ttcgagcggc
    13381 agatcatgat gtccaaacag gagatcaagg atgaattcaa gcagtccgaa ggaagccctg
    13441 agattaaagg ccgtatccga aggatgcagc gtaaaatggc tcaaacacgc atgatgcagg
    13501 ctgttcccga agccgatgtc gtgatccgaa atccaaccca ttacgcaatt gcgttaaaat
    13561 atgaagaaaa aagcggcagc gctccgctgg ttattgcaaa aggaaaagac tacatcgcgt
    13621 tgaaaatcgt tgaaatcgcg attcagaata atgtcagcgt taccgagaac cggcctttgg
    13681 cgcaggctct tttcaaaaca gttgaaatcg gccaggaaat tccttccgag ttttacaagg
    13741 ctgttgccga ggttctggct tatatttaca agttgaaacg cgcaggaaga caataaattc
    13801 acattattcg ccgggaggtg agcggcaatg cttgaaaaaa ttaaaggaag catcgtttcc
    13861 atcgccgtca tttgcgtaat cgttttaatt atattaccgc tgccggcgtt cttattggat
    13921 tttttgttga ttgttaattt gtccctggcc atgctgattc ttttcatcac catgtatacg
    13981 aaaaacgcgc tccagttttc catttttcca tccctcctgc tgattacgac gctgtttcgg
    14041 ctgtcgctcg aaatatcagc caccaggctt attctgacca ataacggcga tgccggccag
    14101 gtcattcaaa cctttggtaa ctttgttata aagggcaata ttcttgttgg actcgtaatt
    14161 tttatcatta tcgttgtcgt taattttatt gtaatcacca agggctctga aagggtggcc
    14221 gaggtggcgg caagatttac ccttgacgcc atgccgggca aacagatggc gattgacgcc
    14281 gatttaaatt ccggcctgat caccgaaatg gacgccaaaa aaagacgcag cgatattcag
    14341 cgggaagcag acttttacgg ctcaatggac ggcgcatcaa aatttgttaa aggcgacgcc
    14401 attgtggcga tcgtcatcgt aattgtaaat atagcgggcg gaatcattgc cggtcttaca
    14461 agcggcgaag atatttcgaa ggttatgtcg tccttcacgc tcgcaaccgt aggtgaaggc
    14521 ctgatggctc agctgcccgc gctgctgatt tccacggcga ccggtattat tgtgaccagg
    14581 gcggcgtcac agggaaatat gagtgaggaa ttatctgccc agctgttctc ccagccgctg
    14641 ttgctcagaa caaccggaat cgttcttctg tgcatgcttc tgcttccggg atttccgaaa
    14701 cccgtgctgc tgctgatcgg cggtatgttc ctttttctga gttttcaggt taagggcaaa
    14761 aagcccgggg aagagaacaa tggagcaacg gtgcaggagc caaaacgact atcggcagcc
    14821 ggttccgaac tggaacgctt taagaatccg gacaatgttt atcagcttct cgaaattgaa
    14881 acgattgaaa tggaatttgg ttacagtatt atccctcttg tggatgaaac ccagggcgga
    14941 acatttgtgg ataaagtagt gatgttccgc aggcagtatg ccattgaaac ggggattgtg
    15001 attccaagtg tccgaatgag ggacaatatt cagctttcca ataatgaata tatcatcaaa
    15061 attcgtggtg aaacagtggc ccagggtgag atattcccca atcagcagat gattatgaat
    15121 ccggtttcag atgattttat aatcgatggg attgatacgg ttgagcccgc gttcggtctt
    15181 aaggcaagat gggtgccgca ggaaaaacgg gaagaagccg aaatggccgg atacacggtg
    15241 attgacccgg cggccgtgat gatgactcat ttatccgaag tgatcaagaa gcatatctac
    15301 gagttgattg gaagaaaaga aatcaacgcc ctcctcgata tgcttaagag aagcaatcag
    15361 tctcttgtgg acgaggtggt tccggccaag ctatcggtgg gagatcttca aaaggtgatc
    15421 cagaatctac tgagggagaa tataccgatc cgggatctgg tgacaattct cgaaacgctt
    15481 gcggatcatg cgcccaaaat taaagatacc gatcttctga ctgaatatgt caggcagagt
    15541 ctgaaaaggt cgatttcccg aaaatttgcc ccggaaggca cactaaacgt actcacggtc
    15601 gatccgcaga tcgaaaaaat gataacctca gcggtcaggc agacggacgg cggaacttat
    15661 ctggcgcttg acccggataa agcccaaacg gtgatcaaca gcctcagcga gcaggtcgcg
    15721 cagtttgaga aggccgggat tcctccggtt gtgcttgcgt ctcctgttgt tcgaatgtac
    15781 tttaaaaaaa tgaccgagca ggtgatgccg gatcttaccg tcgtatcata taacgagttg
    15841 gaaaatgacg tcagaatcca ggcgctcggc gcggttgccg cgtagagcgg atgataaaga
    15901 gagaatcgaa acgaggtgtt agcatgacta cattggttga tgagacaacg gtgtcaaaac
    15961 tatggaggcg gtttaagcaa accgatgaca tcggcctgag gaatgatctg gttctcagtt
    16021 atgcgggtct cgtgaagtca attgtgagaa gaatcgcatc aatctgcgga aattatgcgg
    16081 atattgagga tctcacaagt tatggcatga taggcctgat taaagcggta gaaaagtttg
    16141 acctcgagaa gggtgttacc tttgaaactt tcgcaaccta tcgggtccgg ggagaaatca
    16201 ttgattttat gcggcgaaac gattgggttc ccagaggggt tcggaagaga gcgtgggaca
    16261 tcgaaaacac ttcaaatatc atgaccaacg agctgggccg ggttccatcg gacgatgagc
    16321 ttgccaaaaa gctcgggctt aagaaagaca atctcctcca ggttctctct gaaatcgaac
    16381 gttttaatct tgtctcattt gaagaaatta ttcaggacac ggtcaaaatc gataatcaat
    16441 ttgctgattt tgatacgccc gagggctttc tccaggaaga tgagctgctg gatgttctgg
    16501 ctaaaaccct cgacagcctg ccggaaagag aaaagcttgt gattacactt tattattatg
    16561 aagaactgac cttaaaagaa atcagctcaa ttatcggcgt cagcgaatcc cggatttcac
    16621 agcttcattc cagagcaatc aacaggatga aaaaaacgct gcagtcctat gttaacagct
    16681 aaacagtgag gtgcaaataa catgattcga ggaattttta catcagccac cggcatgatc
    16741 atgcagcaga aaaaactgga tgtgatcggc aacaatatcg ccaatgatca gacatccgga
    16801 tttaaaagcg atgaggtatc ccttcggtca tttgatcagg aaatgatcca gaggatgagt
    16861 gaagatgtcc aggttggaag catgacatac ggtgtgactt cggatgggat caaaaccgat
    16921 ttttcaaatg gaaattacag tcagaccaat tcagctaccg atattgccat tgtcggaaat
    16981 ggttattttg ccgtgcagga cggaaataat ggagtgaaat atacccgtga cggagggttc
    17041 actgttgatt ccgcgggcta tcttgcgctt ccatccggcg aacgtcttct ggggaccaac
    17101 ggccagccgc ttaaagtcag cggagaccag ttttctcttt catcggacgg ggtggtaaga
    17161 aataagtcgg gagccgtagg gcaaattgca gtttatcagc cgaccgggca aattgtaaaa
    17221 cgcggcgacg gttttatgca aatgaccggc gcgagccaga ttaagggaac gctcaggcaa
    17281 gggtttctgg agggttccaa tgttgatatt gttacacaga tgtcgcaaat gatggtggcg
    17341 acccgcagct accagtccaa ccagcagagc tttaagacag ccgatcagac tcttgaaaaa
    17401 ctggtgaatc aggtcggttc aatgagatag ttgccgtgag acaggaggat aatgatgatt
    17461 cgagcttttt atagcggaac agccgggatc aaagcccagc aggaagtggt cgatattgtt
    17521 tccaataata tcgccaacgt caatactgag ggatttaagt caaaatccgg tcagtttggc
    17581 gacttgttgt atacatccat ggaggaaaag acgggaagat ccaaaaccgt ccttgccggc
    17641 agcggagggg ctgtcgctga agtcaaaaat aacatgtccg acggcggttg cacccaaacg
    17701 gggagattgt tggattacaa aatagatgga agcggctttt tccgcgttca ggatgggagc
    17761 ggaaataatt attataccag aaacggagcg ttttcaattg caaatgaaaa cggcgggatg
    17821 tacctcgtaa actccgccgg tatgtctgtg cttgacgcga agggaaacaa gattaaggta
    17881 accggaacgg gcgcggtgca agcacagccg ggcgtttatg atttccctaa tgaatccggc
    17941 cttcataacc agggcaacaa tctgttttca gcctccaaca cttccggggg tgcctatgcg
    18001 gtcgatacga aagtcatgac gggttatctg gaatcatcca acgtcgacct tgctcaggaa
    18061 atgacccggc tgatcatgtc gcaaagagat tatcagctga gtgcgcgagt ggtttcaacc
    18121 gcggatcagc ttgcaaatat ggcaaatgag atgtcctgat gcgcagattt cctttcacca
    18181 actataaaat atgagcggat gctgacgata tatatactga aacctgttga aagcggtgag
    18241 tttcaatatg aaaattgatg gaataaacat acaaaaaata tacgagtcgt tttccaaaaa
    18301 tgcggaatct gctgccgagg aggcctgcgc cacaaagcag gatcaaggcg ccgatccgaa
    18361 agacagagtg gaaatttcgt caaaagcgtc cgatatgtcg tttgcaagaa agactgccca
    18421 gaaacagata ggcgtcgagt ctgagcagga cagggaagca agaatattcc aaattaaaaa
    18481 tcaaatcgaa agcggccaat attccatttc ttcccgcgat gttgccgcaa gcatcgtcaa
    18541 aggaagaaat tttgacaaaa acgtttaagg aggccggaag atgccgtctg cagactggga
    18601 aacggagctc gtcaatcttc ttgaaaaaga acgtaaaatt ctgacgaggt ttatggatat
    18661 cgcacatcag aaaactgagg cgctgacggg cggaaatatt tccgagattg acaagtttgt
    18721 gaatatcgag cagccgcttg tcgtccagat gagtgctctc gaatcgggaa gaatcagtct
    18781 tttgggcaag aaaaattttc agggcctgac gcttagtcag cttgttgaaa aatccggcca
    18841 aaagaaaagt gaactactgg aactttttga ttccatgaac agtcttgtca gcgaacttcg
    18901 ggcagccaac gacctaaaca gcaacattac aaaatccaga attgaatttt ataatttttt
    18961 aagcggaaaa aagcaaaaga agaatcttta tgaaaatagc ggccgtgtta agggcgtttc
    19021 cgaaaaaaca ggcctgattg atagaaaaat ttaaggactt aattgcgtga ggggtcaatt
    19081 ccgcttaacg caatgaacca tgacatctat ttagggagtg atacaaatgc catccacttt
    19141 tgccggtttg gaatctgccc gctcggggct aacagccagc cagtacggtt tggatgtgac
    19201 cagtcagaat gttgccaatg caggcacgac gggctattca aggcagacat tgaatatgac
    19261 ttctgcaggc ccttatgtcg gtgcttaccg gacgctgccc gccggcacgc tgttgggcca
    19321 gggtgtaaat atcggcggta ccgatcaggt cagggatgaa tttcttgata cccgttaccg
    19381 cagtcaaaat gctacttata atgaatggaa gaatttgagt gatggcctga cttctgtgga
    19441 agatgtcgtg aatgagttta ccagcgatgg aagcggaacg attgtcggta tcagcggcca
    19501 gctgaaagat cttgtcgatg accttcagag ctattccaat acaccggatg acgcgaacct
    19561 tccgacgacc gttaaatcag acttccaaac tctttgccag tcgattcgga cggcatacag
    19621 ttcattggaa tctctctctt
//
LOCUS       DATALY010000124         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_289462,
            whole genome shotgun sequence.
ACCESSION   DATALY010000124 DATALY010000000
VERSION     DATALY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289462"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHR42_03775"
     CDS             <1..399
                     /locus_tag="VHR42_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3026331.1"
                     /translation="KAEGFAALEDDRITGLATYFVTGSVCEITSLDSLVEHQGVGTAL
                     VNKIIEAAKGRGCSKIVLITTNDNLNAMRFYQKRGFDMAHLYRNALDASRKLKPEIPL
                     VGDDGIPLRHEIECEFDLGHRHSPGERSNS"
     gene            396..1613
                     /locus_tag="VHR42_03780"
     CDS             396..1613
                     /locus_tag="VHR42_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3026332.1"
                     /translation="MKKHYFSAYAGLPREVYFLFAARLVNAMGSFIMPLLTLILTQKL
                     GHTKSDAGGMISFLILTEAPCLILGGKLTDSIGRKKIIVICSLAGAFLYILCGLLEQG
                     NMMVLFIVLASDFYTITFPAFNAMLADLTKQENRRSAFSLMYFGMNIGMSISPIIGGL
                     LFKDHLQLLFLLDAVTTIGYTIVIARNVPETLRHKKEDTDSPEPPVEKHSLYSVLKCA
                     PILVTFIFLLFLYDFCYSQWNFMLPAQFGDMFGQDGARLFSMLCSANGFTVILLTPIV
                     TSLSHRFRPLAIIAAGGLMYFSAYLGFAFGNNLAMFFFFVELFTLGEIVTTIQIEAFV
                     TGHSPADCRGRISAFENFVRGSAGAFGPLVMGHVLTATGYQKSWLLIAFLIFIGGSGM
                     ILLNRKDRREIPS"
BASE COUNT          408 a          453 c          421 g          511 t
ORIGIN      
        1 aaggcggaag gttttgccgc cttagaagac gaccgtatca ccggccttgc tacctatttc
       61 gtgaccggca gtgtctgtga gattacatcg ctcgacagcc tcgtcgagca ccaaggggtt
      121 ggaaccgcac ttgttaacaa gataatcgaa gccgcgaaag ggcgcggctg ctccaaaatt
      181 gtcttaatca cgacgaacga caatcttaac gcgatgcgct tttaccagaa acgcggattc
      241 gacatggcgc atctctaccg caacgcactc gatgcttcgc ggaaacttaa accggaaatc
      301 ccgctcgtag gcgatgacgg cattcccttg aggcatgaaa ttgaatgtga atttgacctc
      361 gggcaccgac attcgcccgg ggaaaggagc aactcttgaa aaagcattat ttttctgctt
      421 acgcgggtct tccgcgtgaa gtttattttc tttttgcggc aaggctcgtc aacgcgatgg
      481 gttctttcat catgccgctg ctaacactga ttctgacgca aaagctcggc cacacaaaat
      541 ctgatgccgg cggcatgatt tcctttttaa ttctcacgga agcgccgtgc ctaattctgg
      601 gaggtaaact aaccgattcc atcggcagaa aaaagatcat tgtgatctgc agtctcgccg
      661 gcgctttcct ttacatcctc tgcgggttgc tggagcaggg aaacatgatg gttttgttca
      721 ttgttctcgc ctccgacttc tacaccataa cttttccagc gttcaatgcc atgcttgccg
      781 atctgacaaa acaggaaaac agaagatccg ctttttcgct gatgtatttt ggcatgaaca
      841 ttggtatgtc tatcagcccg attatcggcg ggctgctgtt caaagatcat ttgcaacttc
      901 tgttccttct agacgcggta actaccattg gctacacaat cgtcattgcg cgaaatgtgc
      961 cggaaacctt gcgacataaa aaggaggata cggactctcc ggagccgcca gtcgaaaaac
     1021 attctctcta cagcgtgtta aaatgcgccc cgattcttgt aaccttcatc tttttactgt
     1081 ttttgtatga cttctgctat tcgcagtgga atttcatgct cccggcgcag tttggcgaca
     1141 tgttcggtca ggatggtgcc cggctgttca gcatgctttg ctccgcaaac ggttttacag
     1201 tgattctgct gacgcccatc gtgacatcgc tttcccatcg gttccgtccg cttgcaatta
     1261 ttgcggccgg tggactcatg tatttttccg cttacttagg ttttgccttt gggaacaacc
     1321 ttgccatgtt tttctttttc gtcgagctgt ttacactcgg tgaaattgtg acgaccatcc
     1381 agattgaggc gtttgtaacc ggacattccc cggctgactg ccgcggcaga atttccgcct
     1441 ttgaaaactt tgtccgcggc tctgcaggtg cattcggacc gcttgttatg gggcatgttc
     1501 tgactgcgac tggttatcaa aaaagctggc tgttgattgc atttcttatt ttcatcggcg
     1561 gatccgggat gattctgctc aaccggaaag accggcgtga aatcccctca taaatcagtt
     1621 tgcttcaaac ctcctccgac atttctcgtc agaggaggtt tttgaaaaaa ggatggtttt
     1681 atgtccgtca gcaatttact cgtcattgcg gctttcttct ttattctcct ctttgggaac
     1741 aaaatcaaga agctctcctg tctgattcgt gaaacagcgt gagaaaaaga aaa
//
LOCUS       DATALY010000125         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_28480,
            whole genome shotgun sequence.
ACCESSION   DATALY010000125 DATALY010000000
VERSION     DATALY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28480"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHR42_03785"
     CDS             <1..198
                     /locus_tag="VHR42_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX3026333.1"
                     /translation="GIRFSAAGENIAMGQPTPQAVMTAWMNSPGHRSNILSATYNQIG
                     VGVAKNSRGQLYWTQMFIKSM"
     gene            444..1502
                     /locus_tag="VHR42_03790"
     CDS             444..1502
                     /locus_tag="VHR42_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX3026334.1"
                     /translation="MEILRKENLLEYKRFVLSNQKGHFMQGPEWAQVKNNWNSEVVVE
                     RDKSGEIIGGILVLIRRLPGLPFSIMYSPRGPVCDIHDTKTLESLMKGVRNLAKKYRA
                     YVFKMDPDVAASADDFARQLLSMGFERKGGGKNFEEIQPRFVFRLDTGNQTEDEVFAK
                     FHSKTRYNIRVALKRGVEVKIMDRSALKEFMPIMRETGLRDGFPIRPLEYFDRMMQVM
                     GEHARLYMAYYEGKPVAGTIAVQYGDKTWYLYGASSNEYRNIMPNYLLQWEMIRWSVE
                     SGCRVYDFRGVSGDLSESNPLYGLYRFKKGFNGEFTEFVGEFDLILNQPVALLVENGL
                     KFVKKLRLLTNRRHKVKK"
BASE COUNT          423 a          321 c          433 g          387 t
ORIGIN      
        1 ggcatccggt tctcggccgc cggagaaaat atcgccatgg gccagccgac tccgcaggcg
       61 gttatgaccg catggatgaa ttcacccgga caccggagta atattctcag cgcaacttat
      121 aatcagattg gcgttggcgt agcgaaaaac agcaggggcc agctctattg gactcagatg
      181 tttatcaaat caatgtaaaa ttcgtccgat atggaagcag cgccgcaaga ttcagacttg
      241 cggcgctgtt tttggtttac gaagcttcaa acggatacat aatctctata tgaaagaatg
      301 ttgaaatcaa tatcgaagtg acgatattgg atggaaatcg ggtgatccgg ccaacggatc
      361 gaaccattga cattgaaaac atgtggaagg aatgttataa tttttatatt gcaaagcctt
      421 tgtgggctga aggagtgtat tgattggaaa tactgaggaa agaaaatctt ttggaatata
      481 aacggtttgt attgtcaaat caaaaaggtc attttatgca gggacccgaa tgggcgcagg
      541 ttaaaaataa ctggaactcg gaagtggtgg tcgagcggga taaaagcggt gaaattattg
      601 ggggtattct ggttttgatc cgccgccttc ccgggcttcc gttttcgatt atgtattccc
      661 cgcgcgggcc ggtctgtgat attcacgaca cgaaaacgct ggaatcgctg atgaaaggcg
      721 tccggaatct cgccaaaaaa tacagggctt atgtgtttaa aatggacccg gacgtcgcgg
      781 cttcggccga tgattttgcc cgtcagcttc tttcgatggg atttgaacgt aagggcggcg
      841 gcaagaactt tgaagagatc cagccccggt ttgtctttcg gcttgacacc ggaaaccaga
      901 cggaagacga ggtctttgcg aagtttcatt ccaaaacgcg ttataacatc cgggtcgcgc
      961 tcaagcgcgg cgtcgaagtg aaaataatgg accgttctgc tctgaaagag tttatgccca
     1021 ttatgaggga aaccggcctg cgcgacgggt tccccatcag gccgctggag tattttgacc
     1081 gaatgatgca ggtgatgggc gaacacgcgc ggctttacat ggcgtattat gagggcaaac
     1141 cggttgcggg aaccattgcc gtccagtatg gggacaagac ctggtatctt tacggcgcgt
     1201 cttcgaacga ataccgcaat atcatgccca actatctgct tcaatgggaa atgatccggt
     1261 ggtcggtcga atccggatgc cgggtatacg atttcagggg agtgtcggga gacttaagcg
     1321 agagcaatcc gctttatggg ctttatcgct ttaaaaaagg gtttaacggc gagtttaccg
     1381 aatttgtggg tgagtttgat ctcattctga atcaaccggt ggcgcttctt gtcgaaaacg
     1441 gtcttaaatt tgtaaaaaaa ctgcggcttt tgaccaacag gcgtcataag gtgaaaaaat
     1501 aaagaaaagc cgccagagac ggggaagtga aaattatggc cgcattggtc attgatcgaa
     1561 aaga
//
LOCUS       DATALY010000126         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_374029,
            whole genome shotgun sequence.
ACCESSION   DATALY010000126 DATALY010000000
VERSION     DATALY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374029"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..995
                     /locus_tag="VHR42_03795"
                     /pseudo
     CDS             48..995
                     /locus_tag="VHR42_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
     gene            complement(909..1682)
                     /gene="cmk"
                     /locus_tag="VHR42_03800"
     CDS             complement(909..1682)
                     /gene="cmk"
                     /locus_tag="VHR42_03800"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX3026335.1"
                     /translation="MRRRRLSLPCPASDKRRFCWPMVNIALDGPAGAGKSTLAKMLAN
                     KLGYIYVDTGALYRSIGLYAVRNHAETKDSAAVSSLLNDLKIEIRHKDGHQCVVLNGE
                     DVTGQIRSPEISMAASDVSAIPKVREFLLGLQKDIAAGNDVVMDGRDIGTVVLPDAKI
                     KIFLTASPEDRARRRYEELRAKGADVDYGDVFRDVIRRDEQDSGREIAPLRPADDAVI
                     VDTTGLVRLGGSRYSGKPDRNRYQLSGQQLFQRIAEILV"
     gene            complement(1689..>1904)
                     /locus_tag="VHR42_03805"
     CDS             complement(1689..>1904)
                     /locus_tag="VHR42_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3026336.1"
                     /translation="AKSFRPIEEAIVTSGGVSVREVSPKTMESKLLKGLYFAGEILDV
                     DAHTGGFNLQIAFSTGYTAGTSAAAQS"
BASE COUNT          447 a          513 c          515 g          429 t
ORIGIN      
        1 atctgggatg aatacaaggt ttatgaaaag attgaggatc aacgctgatg aaatgcatcg
       61 ttgttgccgg cgcgggcgcc gccggaatgg tggcggccgg atttgccgca aaaagcggct
      121 gccgtgtcat cgtaatcgaa aaaaatcaac gccccggcag aaaactgatg attaccggaa
      181 aagggcgctg caatgtcaca aacgactgcg acaatgacga gtttctggcg aatgttccgg
      241 ttaacgggaa atttctttat ggagccattg ccaaattcgg ccctcggcag acaatggagt
      301 tttttgaaaa tctgggcgta gcgcttaaag tagagcgcgg caaacgggtt ttcccacgat
      361 ccgaccgggc cgtggatatt gtggatgccc tgcaccgttt tgtcaccgaa ggcggctgtg
      421 aaatccgtca gggcagcgtt tccggtctta caatcgaaga cggatgctta aaaggcgtgg
      481 ttttgcaaaa tggcgcaaaa gtagaagcgg acgccgtgat actctgcacg tgcggcatgt
      541 cgtatccgct gacagggtca accggcgacg gttattctgt cgcgcgggga gccgggcata
      601 cggtcattga tccaaagccg tcccttgttc ccatcgaaac ggcggaactc tggccggtgg
      661 agctgcaggg gctttctctg aaaaacgtcc gggtcacggt ggcgaaagcc ggcggttcca
      721 agccggtatt tgaagaaacg ggcgagatgc tttttactca cttcggcgtt tccggaccgc
      781 tgattctcag tgcgagctcc catatgagag atttaacaaa aaaccggtat gaaatcagaa
      841 tcgatttaaa gccgggattg acggtcgaac agctggatgc caggctgcag cgtgattttt
      901 tgaaattttc aaacaaggat ttcagcaatt cgctggaaga gctgctgccc agaaagctga
      961 taccggttgc ggtccggctt tccggaatac cggcttccac caagacgaac cagacctgtg
     1021 gtatccacga tcaccgcgtc gtccgcagga cgaagaggcg cgatctcgcg accggaatcc
     1081 tgctcgtcgc gacggataac gtcccggaaa acatccccgt agtccacgtc cgcgcctttg
     1141 gcgcgaagct cctcataacg gcgccttgcc cggtcctccg gagaagcggt caggaatatt
     1201 tttattttcg cgtcgggtaa aacaaccgtg ccgatatccc ttccgtccat cacgacgtca
     1261 ttgcccgcgg caatatcttt ctgaagcccc agcagaaatt cccgtacttt cgggatggcg
     1321 gaaacatccg acgccgccat tgaaatttca ggagacctta tttgaccggt cacgtcctcg
     1381 ccgtttaaga caacacactg atgaccgtct ttatgccgaa tttcaatctt cagatcattt
     1441 aaaagcgacg acacggcggc tgagtctttt gtttccgcgt gatttctcac cgcatatagc
     1501 ccgatggaac ggtaaagcgc gccggtatcc acataaatat agcccagctt atttgcaagc
     1561 attttcgcca gggtgctctt ccccgccccg gccgggccgt ctaatgcaat attgaccatt
     1621 ggccagcaaa acctccgttt gtccgatgcc gggcatggca ggcttagccg ccgccggcgc
     1681 atcgcgtatt aggactgcgc tgcggcggat gttcccgccg tataacccgt agaaaaggcg
     1741 atctggagat tgaaaccgcc cgtatgcgcg tcaacatcca gaatttctcc cgcaaaataa
     1801 agccctttta aaagtttgga ctccatcgtt ttggggctga cctcccggac gctgacgccg
     1861 cctgaagtaa caattgcttc ttcaatcggc cggaacgact ttgc
//
LOCUS       DATALY010000127         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_634303,
            whole genome shotgun sequence.
ACCESSION   DATALY010000127 DATALY010000000
VERSION     DATALY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634303"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..713
                     /locus_tag="VHR42_03810"
     CDS             417..713
                     /locus_tag="VHR42_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026337.1"
                     /translation="MTDPDDRFLARYEQFEKTSADLAWIEQALILVQDEEDFPIIELR
                     YFEHLNPDTHKAYTWEDIAEILDYTDDTQVRRRRNKLIREIALYLFGTEAIINA"
     gene            985..1443
                     /locus_tag="VHR42_03815"
     CDS             985..1443
                     /locus_tag="VHR42_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026338.1"
                     /translation="MTEEIFNILKAAGISGDAAESIAHQISNVVGKETVPKREFNSKS
                     ILARSLQEELRKMATESENQKKSAASAELLRKQFIRHIKKSNSDLKELETRLKILECR
                     TIPKSRQSMFCQFHKKQTNTLTNNAPCFKKRGAGNARRKIKLVIHGASHI"
     gene            1471..1854
                     /locus_tag="VHR42_03820"
     CDS             1471..1854
                     /locus_tag="VHR42_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026339.1"
                     /translation="MYDMKDQINTLLSGISEAHVSYIHAQQMTSLPVITFCEYNNCEV
                     ERQGGKEYLSKIVIQIDLYESSGDHLSSLAATVNNLIYTTGLRRSYTRETYDSQTNLF
                     HKSMLFKGIIQPDTGVVYQNYKEEK"
     gene            1855..2280
                     /locus_tag="VHR42_03825"
     CDS             1855..2280
                     /locus_tag="VHR42_03825"
                     /inference="COORDINATES: protein motif:HMM:NF027776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HEX3026340.1"
                     /translation="MAIATNGTKLYAKKMADTTYTELTGMKDFTDMGAKADRIETTTL
                     GSTVKTYIRGLDDTPELEFTFNFENASDATSAFAICKGLASEKNDVDFKLEFPDGTFY
                     SWSGDCSIYVKAGKVNAVLEFHLGVTATKPIVLNAAAGN"
     gene            2301..2630
                     /locus_tag="VHR42_03830"
     CDS             2301..2630
                     /locus_tag="VHR42_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026341.1"
                     /translation="MKIITIGDKDYSIEYTFNSMCEMEEKTSKSITTFENMSFSTVRA
                     FLWAGLLELNHSVSLSQTGKLVEKWCNENNKSYMDLGALLLDEATQSGFFGEALAMNS
                     PETATEA"
     gene            2753..2971
                     /locus_tag="VHR42_03835"
     CDS             2753..2971
                     /locus_tag="VHR42_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026342.1"
                     /translation="MKSEEANVHAWLIGAYVRIAVATALDSNLEYPELPFGVEPEETD
                     QTWQVSKMKMAGFAAKMEKKLKKTEADH"
BASE COUNT          930 a          664 c          712 g          762 t
ORIGIN      
        1 aaacgaattg gtgactttgc gtttcggtcc atcgtcataa tggatgcctc cccggttttt
       61 ccggatgatt ttcgtactga ttaattccgg attttgatct gtttatctga attttcgaac
      121 attatctgac cgagaatgat cgactgactc ggctaatccg cggtatggcg atggaccgaa
      181 acgcaaagtc accaattcgt tttgagaagg caatcaagac gacttttgaa accgcgtctt
      241 cagaaactgt tcctcgtaaa cgttcaaatg attcgaattg ttttaaaaaa accgagcaga
      301 ttctgtatgc atacccaaaa ctgaagcgtc agttagcctc ttttaaagat tatgttgaaa
      361 ccgtattcca caaacgatcc aagtccgttg taaaagtgat cggagggaac cctcccgtga
      421 cagatcctga cgaccgcttt ctggcaagat atgagcaatt tgaaaaaacc agcgctgatc
      481 ttgcatggat tgagcaggca ctgattctcg ttcaggatga agaggacttt ccgatcatcg
      541 agctgcgcta ttttgaacat ttaaacccgg acacccataa agcctacacc tgggaagaca
      601 tcgcggaaat ccttgactac acggatgaca cgcaggtgcg ccgccgcaga aacaagctga
      661 tccgcgaaat cgccctgtat ctgtttggaa ccgaagcaat tattaatgcc tgaaccgcct
      721 ttcgcgcgcc gaaagggcgc cgatttcgag tctggaatct gccggattac cgggttagaa
      781 taataacatg gaaatttatg aaaaccggcg gcactgtgaa cttttccgat aagggcgcgc
      841 agtgtcaacc cgtccatgta atccgaaaca agacaaaaac atccgtcatg aataatgcgg
      901 atgtttttat agattgccgt gacgccgggc tacggcgtat tcctacgctg gaggcaacca
      961 gctgaaaaag ccggggagga agctatgact gaagaaattt ttaatattct gaaagcagcc
     1021 ggcatatccg gagacgctgc tgaatcgatt gcacatcaga tttccaatgt cgtcggcaag
     1081 gaaacagttc caaaacggga gtttaattcg aaaagcattt tagcaagaag ccttcaggaa
     1141 gaattgcgta aaatggccac ggaatcagaa aaccaaaaaa agtctgcagc ttctgccgaa
     1201 ttacttcgaa aacagtttat ccgtcatata aagaagagta attcggattt aaaagaatta
     1261 gagacaagat taaaaatcct tgaatgccga acgataccaa aatctaggca atcgatgttt
     1321 tgccaatttc ataagaaaca aaccaacact ctcacgaata acgcaccttg ttttaaaaaa
     1381 agaggggctg gaaacgcgag acgaaaaatc aagcttgtga ttcatggggc gtcccatatt
     1441 taaaatttat gccttttggt tggtggtaat atgtatgata tgaaagacca gatcaatact
     1501 ctgctgtccg gcatcagcga agcccatgtt tcttacatcc atgcccagca gatgaccagc
     1561 cttccggtaa ttaccttttg tgaatacaac aattgtgagg ttgaacgcca ggggggcaaa
     1621 gagtatttaa gtaaaattgt gattcagatt gatctttacg aatcaagcgg agatcattta
     1681 tcaagtctgg cggcaaccgt gaacaacctg atctatacca cgggacttcg ccgcagttat
     1741 accagagaaa cctacgattc ccaaaccaac ctgtttcaca aaagcatgct gttcaaaggc
     1801 atcattcagc ctgataccgg agtggtgtat cagaattata aggaggaaaa ataaatggca
     1861 atcgctacaa acggaacaaa gctttatgca aaaaaaatgg cggacacgac ctataccgag
     1921 cttacgggaa tgaaagactt tacggacatg ggcgcgaaag cggaccgtat tgagacaact
     1981 acgctcggaa gcaccgttaa aacttatatc agagggcttg acgacacacc ggaactcgaa
     2041 tttaccttta attttgaaaa cgcaagcgat gctacttcgg cgttcgccat ctgcaagggg
     2101 cttgcttccg agaagaatga cgttgatttc aaactcgaat ttccggatgg aaccttttat
     2161 tcctggtccg gcgactgcag catttatgtt aaagccggca aggtaaacgc cgttctggaa
     2221 ttccatctgg gtgttaccgc aaccaaaccc attgtcttga acgccgctgc gggcaactaa
     2281 attttaagga ggatatgacg atgaagatca ttacaatagg cgataaagat tactcgatcg
     2341 aatatacatt taactccatg tgtgaaatgg aggagaaaac ctccaagagc attaccacgt
     2401 ttgaaaacat gtctttctct accgttagag cctttctatg ggcagggtta ttggaactga
     2461 atcacagcgt ttctctttct cagacaggaa agcttgtgga aaaatggtgc aatgaaaaca
     2521 ataaatctta tatggatctg ggtgctcttt tattggacga ggcaacgcaa tcgggttttt
     2581 tcggggaagc tcttgcgatg aacagtccgg agacggcaac ggaagcctaa aggagatctt
     2641 ccgtcatatc tgggaggatg cggcagctta tggggttgct gcatcctctt tctggcatat
     2701 gactccggct gagttagaaa cgcattgcaa gggttttgaa aaacgcttaa agatgaaatc
     2761 ggaagaggca aacgtacacg cgtggctgat tggcgcttat gtaagaattg cggttgcaac
     2821 cgctctggat tccaatcttg agtatccgga actgcctttc ggcgtcgaac ccgaagaaac
     2881 cgatcagacg tggcaggttt ccaaaatgaa aatggcgggc ttcgcagcca agatggaaaa
     2941 aaagctgaaa aagacggaag cggatcatta gccgtcggct atttgaccca aaaatccagc
     3001 cccgctttta aagcgcgagg gatgaatcta ttttttcctc attctttcat tggacattgg
     3061 aaatatct
//
LOCUS       DATALY010000128         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_719159,
            whole genome shotgun sequence.
ACCESSION   DATALY010000128 DATALY010000000
VERSION     DATALY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719159"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..678)
                     /locus_tag="VHR42_03840"
     CDS             complement(73..678)
                     /locus_tag="VHR42_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3026343.1"
                     /translation="MKKRVLAVLLALSLAMAVFSGCSSAKGTENSGSNGTSSSAQKDV
                     TLTFTYDQDGSHDPLEKWMSDKKVISRFEAENPGVHIKLVPISSTDGDYATLLALKLT
                     SDRTAPDVFMEDTYMTATDAAAGHLACLDDYLSKWTDYSNYMDGAKAAVKGMDGKSYG
                     VPISTDARGLFYNIDVLKKAGINTPWQPKTGNDILDKMELE"
     gene            1044..1574
                     /locus_tag="VHR42_03845"
     CDS             1044..1574
                     /locus_tag="VHR42_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026344.1"
                     /translation="MKKLHTGRFGRHAAIFLLLSAAIFTFFCSAFASVLVRVTYSGTD
                     WSQGQSVDTFNGVPAKYTTVKSSTGDFCCAGYVKKYYRARFGVTVTNLLTGRTPVSSS
                     GSFSRTWAPKPGDVGYQLDRSGSGHWFIVKSVSGTTCTVIEQNWKWTTDGKTYCYTNR
                     TVNPSTPGIKYFRLKK"
     gene            1715..1939
                     /locus_tag="VHR42_03850"
     CDS             1715..1939
                     /locus_tag="VHR42_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEX3026345.1"
                     /translation="MPGIEFEVVKEFGELSENIKGWKREVNLVSWSGRDPKYDIRDWS
                     PDHSKMGKGITLTADEMKQLRDILDNMELE"
     gene            complement(2026..>3183)
                     /locus_tag="VHR42_03855"
     CDS             complement(2026..>3183)
                     /locus_tag="VHR42_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3026346.1"
                     /translation="LINKYGKSVPIADSAAPIRDKSGTIFGVVMVFRDVTLEKAQENR
                     ILFLSYHDALTGLYNRRYLESEARRILSAEELPITVVMGDVNGLKITNDVFGHKFGDS
                     LLQDVAKVLRRNCRSGDIIIRWGGDEFVMILPGTGEEQAERRLEKIRLDCEKHSGNDR
                     IMSVSTGYAVLHTRSEDLERKLQEAEEAMYHQKLLAGKSYRNTIISALLATLNEKSME
                     TEEHAQRLQLFCQEIGKELHLSENEMNDLSLLAILHDIGKVGVPKHILEKSGPLSPEE
                     WKEMRKHSEIGYRIAQTTPELSKVSEYILAHHERWDGTGYPGGLKEDQIPLLCRILAV
                     ADAYDAMTNDRVYRKALSQEAAKQELLSNAGTQFDPHVVDLFFKICENSSH"
BASE COUNT          743 a          805 c          821 g          814 t
ORIGIN      
        1 ataagtaccg gcttgtaaaa cggatatgca aaaacgcctg gaaagcaaaa atgctttcca
       61 ggcgttttct gttcattcca gttccatttt gtccagaatg tcattcccgg ttttaggctg
      121 ccacggggta ttgattcccg cttttttcaa tacgtcaata ttgtaaaaaa gacctcttgc
      181 atctgtggag atgggaacgc cgtaggattt tccatccatg ccttttacgg cggcttttgc
      241 cccgtccatg tagttgctgt aatcggtcca tttggaaaga taatcgtcta agcacgccag
      301 atgtcctgcc gccgcgtccg ttgccgtcat ataggtatct tccatgaaaa catccggcgc
      361 cgtccggtca gaggtcagtt tcaaagccaa taaggtagcg taatcaccgt cggtagatga
      421 gatcggaacg agtttgatat gtacgcccgg gttttccgct tcaaagcggc tgattacctt
      481 tttatcggac atccattttt ctaacggatc atgagaacca tcctgatcgt aagtaaatgt
      541 caaggttacg tctttctgtg cagaggatga tgttccgttg cttccactgt tttctgtacc
      601 cttcgcggat gaacagccgc taaacactgc catcgccaaa gaaagtgcca gaagaactgc
      661 aagaaccctt tttttcataa cgttctcctt ttaaatattg ggatttgtaa ataaacagca
      721 agtaccagta tagagttctt tcaatcattt cctattatgc tggtattaaa aataagttaa
      781 ttattcaaaa gctgcctgtt tatgatcgat ttctatctgg tttggtagtc ctcccttcac
      841 agcagagaac agctgttgga tataaatgga agaatactcc attatttgtt aatccttaca
      901 aaacggttaa gaaaactgcg aaaaatggta taaagaaaac agaaagaagc aatttgcaca
      961 gggaggtgac ggaatcgacg taaaaattca ggcttcttac ataaaaagct cgaacaacgg
     1021 atttgcaaca caggggaatg aatatgaaaa agctacatac tggccgcttt gggcggcatg
     1081 cggcaatctt tctgctgctt tccgccgcga tttttacttt tttctgctcc gccttcgcct
     1141 ccgtgctggt gcgggtgacc tactccggaa ccgactggtc gcagggccag tcggtcgaca
     1201 cgttcaacgg cgtaccggcg aaatatacga ccgtaaaaag cagcacgggt gatttctgct
     1261 gcgccggata cgtgaaaaag tattatcgag cgcggttcgg cgtcaccgtg acaaatctgc
     1321 tgaccggccg cacacccgtt tcgtcctccg gttccttttc ccgcacctgg gcgccgaaac
     1381 ccggcgacgt cggctatcag cttgaccgct cgggttcggg ccactggttc attgtaaaga
     1441 gcgtttcggg tacgacctgc accgtgatcg aacagaactg gaaatggacc accgacggca
     1501 agacctactg ctatacgaac cgcacggtga atccttctac tcccggaatc aaatacttcc
     1561 gtctgaaaaa atagtctctt ttcaccgccg cccggctcac gtcgggggcg gttttttgcg
     1621 ccccaggttg cggcgggaaa ttttccggtt gtaaaaatcc cccgatccgt tataataata
     1681 gagcgaaaat aaaagcaaaa ggaaggaatc gtttatgccg ggtattgagt ttgaagttgt
     1741 aaaagagttc ggggagcttt ccgaaaacat aaagggatgg aagcgcgagg tgaacctcgt
     1801 gagctggagc gggcgtgacc cgaagtacga catccgcgac tggtcgcccg accactcgaa
     1861 gatgggcaag gggatcaccc ttaccgcgga cgaaatgaag cagctgcgcg acattttgga
     1921 caacatggaa ctggaatgaa cagaaaacgc ctggaaagca tttttgcttt ccaggcgttt
     1981 ttgcatatcc gttttacaag ccggtactta ttaggcggcg aaaattcaat ggctcgagtt
     2041 ttcgcagatt ttgaaaaaca ggtccacaac gtgggggtca aactgtgtgc cggcgttgga
     2101 aagcagctct tgcttcgcgg cttcctgaga gagggccttc cggtaaacgc ggtcgttggt
     2161 catggcgtcg taggcgtcgg caaccgcgag gatgcggcac agcaggggaa tctgatcttc
     2221 cttcaggccg ccggggtaac cggtgccgtc ccagcgctcg tggtgggcga gaatatactc
     2281 cgaaaccttt gagagctccg gcgtggtctg tgcgatccgg tagccgattt cggaatgttt
     2341 ccgcatttcc ttccattcct cgggggaaag cgggcctgat ttttccagga tatgtttcgg
     2401 cacgccgacc tttccaatat cgtgaagaat ggcgagcaga gaaagatcgt tcatctcatt
     2461 ttccgaaagg tgaagctcct tgccgatttc ctgacagaac agctgcaaac gctgcgcgtg
     2521 ttcctcggtt tccatgcttt tctcgttaag tgtggcgagc agagcgctga tgatggtgtt
     2581 gcggtagctt tttcccgcca aaagcttctg gtggtacatg gcttcctcgg cctcctgcag
     2641 cttgcgctcc agatcctcgc tgcgggtgtg cagcacggca tatccggtgg aaacgctcat
     2701 aatccggtcg ttcccgctgt gcttttcgca gtctaagcgg attttttcca gccgccgttc
     2761 ggcctgttcc tccccggtgc cgggcagaat catgacgaat tcgtcgcccc cccagcggat
     2821 gatgatatcg ccgctgcggc aattccgccg gagcaccttt gcgacgtcct gcagcagcga
     2881 gtcgccaaat ttatgcccga acacatcgtt cgttattttc aggccgttga catcccccat
     2941 gaccaccgta atgggcagct cttccgctga aagaatccgg cgggcttcgc tctccagata
     3001 cctgcggttg tacaggcccg tcagagcgtc gtggtagctc aggaaaagaa ttcggttttc
     3061 ctgagctttt tcgagggtga cgtcccggaa aaccatgaca acgccgaaga ttgttccgct
     3121 tttatctcgg atcggtgccg cgctgtccgc aatcggaacg gatttgccgt acttgttgat
     3181 gag
//
LOCUS       DATALY010000129         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_719178,
            whole genome shotgun sequence.
ACCESSION   DATALY010000129 DATALY010000000
VERSION     DATALY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719178"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VHR42_03860"
     CDS             complement(<1..676)
                     /locus_tag="VHR42_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026347.1"
                     /translation="MDIESLLFDLSVSDCMLPGEEGHRKALNELRKLCPEACSDNLGN
                     IIAPVFPTPAGGEHILIDAHLDEIGFVVTTIDPDGFLRVNKVGGPDMRTLLGHEVTVH
                     GKKTLYGVFCCQPPHLTSRADYKKAAEISEIAIDVGLSHDKACELVSPGDTVFLRRNP
                     AKMLGGLVTGKAFDNRAGVVSILRCIDLLKGQKPGAGLSVIFSLSEETSFKAVSGGTF
                     AVAPTQA"
     gene            complement(771..2165)
                     /gene="murD"
                     /locus_tag="VHR42_03865"
     CDS             complement(771..2165)
                     /gene="murD"
                     /locus_tag="VHR42_03865"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX3026348.1"
                     /translation="MNDRLKGFYNYLEGRRIAVLGIGMSNTPLVKMFLSKAGHVIACD
                     RRSRDQLGSIAEELEKAGAELRLGDGYLNDLNVDIIFRTPGMKYNLPQLEKARRQGIV
                     VTSEMEVFFDLCPGRILAVTGSDGKTTTTTLIAKMLQKQGYTIHVGGNIGHPLLPEIE
                     TVKPDDMAVVELSSFQLISMRKSPDIAVVTNVTPNHLDIHKDMEEYISAKRNVLLHQN
                     AFSRTVLNLDNKTTAAMAEQTRGQCLMFSRRAKVEYGSYLSDNGDILMADTAGTHKVI
                     NQADIMIPGLHNVENYLAAIAATWGLVTPETVRDVARSFAGVEHRIEFVREIGGAKYY
                     NDSIATSPTRTIAGLNSFREKVILIAGGYDKHIPFTALGPKVTEKVKYLILLGKTADK
                     IEEAVRSAPDYSAETLPIIRVESLEQAVSAARNYARAGDIVTLSPACASFDMFKNFEQ
                     RGDAYKEIVNSLPA"
     gene            2414..2761
                     /gene="rnpB"
                     /locus_tag="VHR42_03870"
     ncRNA           2414..2761
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHR42_03870"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          696 a          752 c          795 g          743 t
ORIGIN      
        1 tcgcctgagt tggagcaacg gcaaaagtgc cgccggatac ggctttgaaa ctggtttcct
       61 cgctcagact gaatataacg ctcaggcctg ctccgggctt ctgccctttc agcagatcaa
      121 tgcagcgcag aatcgatacg acgccggccc ggttgtcaaa ggctttgccc gtaacaagac
      181 cgcccagcat cttcgccggg tttctccgaa ggaaaaccgt atcgccgggt gataccagtt
      241 cgcaggcttt gtcgtgagac aggccgacgt ctatggcaat ttcggagatt tcggcggcct
      301 ttttgtagtc tgcacggctg gtgagatgcg gcggctggca acagaatacg ccgtaaagag
      361 tcttcttgcc atgcactgta acctcgtggc ccagaagcgt tctcatatcg gggcccccga
      421 ccttattcac gcgtaaaaaa ccatccggat caatggttgt caccacaaaa ccgatttcat
      481 ccagatgggc gtcgatcaga atatgttctc cgcccgcagg cgtgggaaac accggggcga
      541 taatgttccc gagattatcg gagcaggctt ccgggcagag cttgcgcagc tcattcaggg
      601 ccttccggtg gccttcttcg ccggggagca tgcagtctga cacagaaaga tcaaataaca
      661 gtgattcgat atccatagca attaaaacct tccttttact tataatgtat tcgcgcgagc
      721 cagtccggcg gaaggttgcg cgcagcgttt ctttgaagca gcggcccgaa tcaggccggc
      781 agagagttga cgatttcttt ataagcgtcg ccgcgctgtt caaagttttt aaacatatca
      841 aaacttgcgc aggccggcga gagagtgacg atatccccgg cacgggcata gtttctggcg
      901 gcggacacag cttgttcgag gctttccacc cggatgatcg gcagggtttc agctgaataa
      961 tccggagcag accgaacagc ctcttcgatt ttatcggctg tttttcccag aagaatcagg
     1021 tatttcactt tttccgttac cttggggccg agcgctgtga agggaatatg cttatcataa
     1081 ccccccgcga tcagaatcac cttttcccgg aacgaattga gcccggcaat cgttcgggtc
     1141 gggctggtcg cgatggaatc gttgtagtat ttggccccgc cgatttcccg cacgaattcg
     1201 atccgatgtt ccactccggc aaaactgcgg gcgacgtcac gaaccgtttc gggggtcaca
     1261 aggccccagg ttgcagcgat cgcggcaaga tagttttcta cgttgtgaag gccgggtatc
     1321 ataatatcgg cctgattgat cactttatgg gttcccgccg tatcggccat cagaatatcg
     1381 ccgttatctg agaggtaact gccgtattcc acttttgccc tgcggctgaa catcagacat
     1441 tgcccgcgtg tctgctccgc cattgccgcc gtggttttat tgtcaaggtt gaggacggtg
     1501 cggctgaacg cgttttgatg aagcagaacg ttccgtttcg cgctgatgta ttcctccata
     1561 tccttgtgaa tatccagatg gttcggagtc acattggtca ccactgcgat atcagggctt
     1621 tttctcatgg agataagctg aaagctggat aattcgacta ccgccatatc gtccggcttg
     1681 accgtttcaa tttcgggcag aagcgggtgg ccgatgttgc cgccgacgtg tatggtataa
     1741 ccttgtttct gaagcatttt tgcaatcagc gtggttgtgg tggtcttccc gtccgatccg
     1801 gtcaccgcaa ggattctgcc ggggcacaga tcaaaaaaga cctccatttc gcttgtgacg
     1861 acgatgccct gtctgcgcgc tttctcaagc tggggaagat tatacttcat tcccggcgtg
     1921 cggaaaataa tgtctacgtt aagatcgttt aagtaaccgt cgcccaatct gagctcggct
     1981 ccggcctttt ccaactcttc ggcgatactg ccgagctgat ctctgcttct tctgtcacag
     2041 gcgatcacat ggccggcttt tgaaagaaac atttttacaa gcggcgtgtt gctcatgccg
     2101 atacccagta ccgcaatccg cctgccctca aggtaattgt agaatccctt gagcctgtcg
     2161 ttcatatgct ttccctccgt aagtccaaac tgccgattga ttctttagag ccactgatcc
     2221 attcagggat gtcggtcggg gtttgcggca gtgacagctg tgtgaaagga taaaaggagc
     2281 aatttggttt atagacacta ttatactaat cccggctgga aatatcaatg ttggatattt
     2341 ttgtaaattg gccggaatgc tgccatgatt tgcagtttga cagaaggatt caaacagatt
     2401 ataatatcat ctgcgagcaa gccagacagc tgcgggtgca gtgccgaaag gccgcatctg
     2461 aggaaagtcc gagctccgca gggcaggata gcgggtaacg cccgccggag gcgactccag
     2521 ggaaagtgca acagagatac accgccgggt tttcccggta agggtggaaa ggcgaggtaa
     2581 gagctcaccg gcgcgcagga gactgcgcgg ccatgcaaac cctatccgga gcaacaccgc
     2641 atagagggat aatacgcttg cccggcgtgt ccccgggaag gtggcttgag ccgtgtggca
     2701 acatacggcc gagatagatg gttgtcaaat acagaactcg gcttatagac ttactcgcac
     2761 catgaaagca aacccgcatg aaggctggaa acccagtaat catgcgggtt ttttatactt
     2821 attgcggttt ttctatttgg actaaaaagt taagataaag actcaaaaag acaaatttgt
     2881 gtggactaaa tgtggactac tcactgatag gaatctcggt gctcgaataa atttattgac
     2941 gattgatgcc aaacgatgta aaattatgtt acttaatgat agtggg
//
LOCUS       DATALY010000130         5039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_462525,
            whole genome shotgun sequence.
ACCESSION   DATALY010000130 DATALY010000000
VERSION     DATALY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462525"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VHR42_03875"
     CDS             <1..805
                     /locus_tag="VHR42_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026349.1"
                     /translation="NAALKIASGEFLGFVDSDDWLAPDMCESMIRACERYHADIAVCG
                     YYNTPSPFDRLSCSLKKTAVFDRRQAMRCYLSRPVIGTVIWNKIYRRELFAGLWFVEK
                     RYNEDEFIMHELFHRAKKVVHIGQCKYFYRRTENSLTRSPFSRAKMDLIDAVKTKQNF
                     IKCNYPELYPETIASLARALLELLYEIEVDPNRDQFGREKKFLINFLNKNYCEIAGGA
                     HVGLRLKLLSRIALKNPNLFRAICQVGATEDFVKKIGRKLLINNSERIE"
     gene            complement(727..1041)
                     /locus_tag="VHR42_03880"
     CDS             complement(727..1041)
                     /locus_tag="VHR42_03880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3026350.1"
                     /translation="MENCKISIIVPFYNAESYLRQCIDSILCQTVKSLELILVDDGSI
                     DGSVKICDEFSKKDQRVRMIQTGHEGPGKARNTAHHSILSELFIRSLRPIFLTKSSVA
                     PT"
     gene            complement(1053..2093)
                     /locus_tag="VHR42_03885"
     CDS             complement(1053..2093)
                     /locus_tag="VHR42_03885"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HEX3026351.1"
                     /translation="MAVYVLLMAFLILNRALIPGSSAKSRRNFLILSSIPIILVMGLR
                     NCTIGGNDPVGYYYHFLSAVGTPLPELLSSSRMEPGYIVFVKLLTLISSSPLTLFLAE
                     AAICVFGVCLFIYRNSEQPFLSLFFYVTLGEFAFEMMGFRQSIAMSICLVGLEFVKKK
                     KPIPFLLTVALAACFHISAIVFLPVYFLAYRKLSAVNLLLTLYVIPAVILLNHQITDV
                     ANGVFSSNYGTGANTGGAVVIAVYLITFGAAFVNQKEVFSHRENLACFNLLALGLITY
                     LFRYWTMASERISFYFFFGLLILLPASIKAVPDEKTRRLAACAAVLLAAGLFYYRLTY
                     QPISPYLFFWEQ"
     gene            complement(2110..3276)
                     /locus_tag="VHR42_03890"
     CDS             complement(2110..3276)
                     /locus_tag="VHR42_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026352.1"
                     /translation="MKITFFSNFFNDHQLSLCNALYRQLGQYFVFAATEPMSDERLQM
                     GFEDIGRNYPYVEQLTGPDKFGRCLELASSSDVAIVGSAPKNIVQKRLKEGKLTFYYS
                     ERMFRKGLWQILKPRTFFELYRGIVSQNRQNLYVLCAGAYAAWDFNRLGVDRDHLFRW
                     GYFPQTGSYDVGRLIESKTGPFDATLLWAGRLVPLKHPELAVKCLSYLKSGGWRVRLD
                     FVGSGEMETPLKEMCRDLGLEDTVRFLGMMKPAGVRKAMENSDIFLFTSNKKEGWGAV
                     LNEAMSCGCAVVASSAAGASKFLIQQGENGFLYPDGDQKGLFHKAELLTGNRLKRRQV
                     GAAACRTITELWNADIASQRLIGLCDSLLAAKSVPFFERGPCSRAEILRNPSVI"
     gene            complement(3290..4558)
                     /locus_tag="VHR42_03895"
     CDS             complement(3290..4558)
                     /locus_tag="VHR42_03895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3026353.1"
                     /translation="MKILWIVNMILPEPARHLGIATGPSGSWMEDLSQRIAGAGDCEL
                     AVAAVGGDRFRDFTVGKIRYFLLPGNGKTMMFYHRGLVPFWERVQSLFTPDLVQLHGT
                     EYTHGLVYLRMFPQQRAVVSIQGILSAIKDHELDGMPKFSVFRYRTMRENLRFNGMAE
                     QMLLHRKNAVYEKEILRRVRFANTVDFWDETRVREINPAIRCFPMDYNLREPFYRSEK
                     WDRHKMERHTVFTNQGGIPLKGLHMLLRALTLVKSRIPDVKLYVPGMEEDHGRLVIAN
                     GYCKYLDRLIRDGGLERNICFLGRLDAAQMAGAMRVCNAAVFPSAMENVSTMMRESMM
                     LGIPTIAAFRGGMADFIQDRQSGFLYDFYEYPVLARRLIEIFEDDGLASSLSAAAAEQ
                     AGQAHDPEKNAGKYLGMYREILREVPDQDN"
     gene            complement(4589..>5039)
                     /locus_tag="VHR42_03900"
     CDS             complement(4589..>5039)
                     /locus_tag="VHR42_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026354.1"
                     /translation="LIPKVCFEELGMFQEEIRTVSDYDFWYRMLLAGWRLLYVSEYLV
                     QGRVHPRQVTYRMSGLAEKEFDEFHYQLCRSLYFEKGVRSWKAYWFIGRCALKRGFTG
                     SASLAFHLASDNMPSIFYRLMYLFTRLECFFARRIKSLAKSLFSRLK"
BASE COUNT         1328 a         1328 c         1222 g         1161 t
ORIGIN      
        1 aaacgccgca cttaaaatcg cttcgggtga atttttagga ttcgtggaca gtgacgactg
       61 gctggcgccc gatatgtgcg aatcaatgat tcgcgcctgt gaacgttatc acgctgatat
      121 tgctgtttgc ggctattata atacaccctc gccgttcgac cgtttgtcct gttcgctgaa
      181 aaaaaccgca gtgtttgatc gaaggcaggc tatgagatgc tatctatcca gacctgtaat
      241 cggcaccgtg atctggaata aaatttatcg gcgggagctg ttcgcaggac tgtggtttgt
      301 ggaaaagcgg tacaatgaag acgaattcat catgcatgag ctttttcacc gggcaaaaaa
      361 ggtagtccat attggccagt gtaaatattt ttaccgaagg acggaaaaca gcctgacgag
      421 gtctccgttt tcccgggcga aaatggatct gattgacgcg gtcaaaacaa agcagaattt
      481 tatcaagtgt aattatccgg aattatatcc cgaaacgatt gcttctctgg cgagagcact
      541 gctggaatta ctctatgaaa ttgaagttga tccgaatcga gatcaattcg ggcgggagaa
      601 aaaatttctg atcaatttcc ttaataaaaa ttactgcgaa atagcaggcg gagctcatgt
      661 tgggctgaga ttgaaattgc tttcaaggat tgccctgaaa aatccgaatt tattccgagc
      721 tatttgtcaa gttggggcaa ctgaagattt cgttaaaaaa atcggccgta agcttcttat
      781 aaataactca gaaaggatag aatgatgagc ggtgtttcgg gctttacccg ggccttcgtg
      841 cccggtttgt atcatcctga ctctctgatc ttttttggag aattcatcac aaatttttac
      901 gctgccatca attgagccgt cgtccacaag tatgagttcc aggcttttaa cagtctgaca
      961 taatatcgaa tcaatacatt ggcgtagata tgattccgcg ttgtaaaaag gaacaatgat
     1021 actgatttta cagttttcca tttttgtctc cattactgct cccagaaaaa cagatagggc
     1081 gagataggct gataagtcag ccggtaataa aacagcccgg ccgccagcaa tacggccgcg
     1141 caggcggcaa gcctcctggt cttttcatcc ggcaccgcct taatcgaggc gggcagcaga
     1201 atcagcaggc cgaaaaagaa ataaaagctg atccgttccg acgccatcgt ccagtaacga
     1261 aaaagatagg tgatcagccc gagagccaga agattgaaac acgccaggtt ttcccggtga
     1321 ctgaaaacct ctttctggtt cacaaaagcg gctccaaaag tgatcagata caccgcgatc
     1381 acaaccgctc cgccggtgtt cgcccccgtc ccgtagtttg agctgaaaac cccgtttgcg
     1441 acgtcggtga tctgatgatt caaaaggatg accgctggaa tcacatacag cgtcagcagc
     1501 aggttgaccg cgctgagctt ccgataagca aggaaataga cgggcagaaa tacaatggcg
     1561 gaaatgtgga aacaggcggc gagcgcaacg gtcagtaaaa aggggatcgg ctttttcttt
     1621 ttcacaaact ccagtccgac cagacaaatg ctcatggcga tgctctggcg gaatcccatc
     1681 atttcaaagg cgaattcccc cagagttaca taaaaaaaca ggcttaaaaa gggctgctcc
     1741 gaatttctgt agataaacag acagaccccg aaaacacaga tggccgcttc cgccagaaac
     1801 agagtcagag ggctcgatga aatgagtgtg agcagtttga caaagacgat ataccccggc
     1861 tccattctgc tggagctcag cagctcgggt aacggagtcc cgacggccga caggaaatga
     1921 tagtaataac ccaccggatc attgccgcca atggtgcagt tgcgcaaccc cataaccaga
     1981 atgatcggga tagacgataa aatcagaaaa tttctgcggc ttttggcgga ggatccggga
     2041 atcagcgccc ggtttaaaat cagaaatgcc atcagcagta catatacagc catcgttcag
     2101 cctcgcggtt caaattacgg atgggtttcg aagaatctct gcccgggagc agggccctct
     2161 ttcaaaaaac ggaacggatt tcgccgcaag caggctgtca cagagaccga tcagcctttg
     2221 ggacgcgata tccgcattcc aaagttctgt gatggtgcgg caggccgcag cgccgacttg
     2281 acgccgtttc aggcggttgc cggtcagaag ttcggcctta tggaacaggc ccttttgatc
     2341 cccgtccgga taaagaaagc cgttttcacc ctgttgaatg agaaattttg aagctcccgc
     2401 ggcactgctc gcgacaacgg cgcagccgca gctcatcgct tcattgagaa cggctcccca
     2461 gccttccttt ttattgctgg taaacaggaa aatatccgaa ttctccatcg cttttctgac
     2521 accggcgggc ttcatcattc cgagaaaccg aaccgtatcc tcaagaccca gatcgcggca
     2581 catttctttg agcggcgttt ccatctcacc ggagcccaca aaatccagac gaacccgcca
     2641 gccgccgctt ttcagataag aaaggcactt aacggccagt tccggatgct tgaggggtac
     2701 cagccggcct gcccagagca gcgtcgcgtc aaacgggccc gtttttgatt cgatcagccg
     2761 gccgacatca tagcttccgg tctgcggaaa gtatccccag cgaaaaaggt gatccctgtc
     2821 gacgcccagc cggttaaaat cccaggcggc gtaggcgccg gcacagagaa cgtagagatt
     2881 ttgcctgttc tgtgaaacaa ttccccgata cagctcaaaa aaagtccgcg gttttaaaat
     2941 ctgccagaga cccttccgaa acatccgctc cgaatagtaa aaagtcagtt ttccctcttt
     3001 cagacgcttc tgcacgatat ttttcggagc ggagccgaca atcgccacgt cgctggagga
     3061 agccagttcc agacatcttc cgaatttgtc cggcccggtc agctgttcca catagggata
     3121 attccggcca atatcttcaa agcccatctg taaacgctcg tcgctcatcg gttcggtggc
     3181 ggcaaaaaca aagtattgtc ccaactgacg gtaaagggcg ttgcacagag aaagctgatg
     3241 gtcgttaaaa aaatttgaaa aaaaagtaat tttcatctgc attcgccttt cagttatcct
     3301 gatccggaac ctccctgagt atttcccggt acatcccgag atattttccg gcatttttct
     3361 ccgggtcatg ggcctgaccg gcctgctccg ccgctgcggc ggaaagagag gacgcaagtc
     3421 cgtcgtcttc aaaaatttca attaatctcc gggccagcac cggatattcg taaaaatcat
     3481 ataagaaccc ggattgccgg tcttgaataa aatcggccat tccgcctcgg aatgcggcga
     3541 tcgtcggtat gccaagcatc atcgactccc gcatcatggt cgatacgttc tccattgccg
     3601 aaggaaatac cgccgcgttg cacacccgca ttgctccggc catttgagcg gcgtcgagtc
     3661 tgccgagaaa acaaatgttc cgttccagcc cgccgtcccg gatcagacgg tctaaatact
     3721 tgcaatagcc gttggcaatg accagacggc catgatcttc ttccattccc ggaacataca
     3781 gctttacgtc cgggatacgg cttttcacca gcgtcagagc ccggagcagc atatgaagcc
     3841 ccttgagggg gattcctccc tgattcgtaa aaacggtgtg tctttccatt ttatgacgat
     3901 cccatttttc tgaccggtaa aaaggttccc gcaaattata atccatcgga aagcagcgta
     3961 tggcagggtt gatttctctc acacgcgttt cgtcccaaaa atccacagtg ttggcaaaac
     4021 gcacccgccg gagaatttcc ttctcgtaaa cggcgttttt ccgatgaagc agcatctgct
     4081 cggccatacc gttgaaccgg aggttttccc gcatcgtccg gtatcggaaa accgaaaatt
     4141 tcggcatgcc gtcaagctca tggtctttga tagccgaaag gattccctgg attgagacga
     4201 ccgccctctg ctgcggaaac attctaagat agacaagccc gtgcgtatat tccgttccgt
     4261 gcagctggac aagatcgggc gtgaaaagtg attgcactct ttcccagaag gggacgagcc
     4321 cgcgatgata aaacatcatg gtcttcccgt tgcccgggag caaaaaatac cggatttttc
     4381 ctaccgtgaa atcacggaac cggtctccgc cgactgccgc caccgccagc tcgcagtcac
     4441 cggccccggc aatgcgctgt gaaagatcct ccatccacga gccgctcggc ccggtcgcta
     4501 ttccaagatg acgggccggc tccggcagaa tcatgttgac gatccacaaa atcttcatag
     4561 gcttctccat atcatccttg tcattttttc atttgagtcg ggaaaacaag cttttcgcca
     4621 aagattttat cctgcgcgca aaaaagcatt ccagacgggt gaacagatac atcagacggt
     4681 agaaaatgga cggcatattg tcggaggcaa ggtggaaagc aagcgacgcg ctgccggtaa
     4741 atcctctttt cagagcgcac cggccgatga accagtaggc tttccaactc ctcacgccct
     4801 tctcaaaata aagcgaccgg caaagttggt agtgaaactc gtcgaactcc ttctccgcca
     4861 aaccgctcat ccggtaagtg acctgtctgg gatgcacccg gccctgcacc agatattccg
     4921 aaacatagag cagccgccag ccggcgagca gcatcctgta ccagaagtcg taatccgaaa
     4981 cggttcggat ctcttcctga aacatgccga gttcttcaaa acagactttc gggatcaga
//
LOCUS       DATALY010000131         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_634827,
            whole genome shotgun sequence.
ACCESSION   DATALY010000131 DATALY010000000
VERSION     DATALY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634827"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..817)
                     /locus_tag="VHR42_03905"
     CDS             complement(2..817)
                     /locus_tag="VHR42_03905"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX3026355.1"
                     /translation="MTVLILYFSGTGNTRRIAEDFKACLIKRNLSVIMHSIEERIDLT
                     KISYDYLIIGCPKYYEYPAMLMLDYLKQNLQRREKLIPSMAFCTQASPLKTDFTGFEK
                     LLRKKNHQLTVEASFPYANNMMIFQAFKPTEPSALMENQKDIRKRMEPLLDAFLKGQM
                     SKEDTKAWQRPLIYMVAVTFSKLMPVFAMKFSADESCIHCGLCVKRCPMENIRMVQGN
                     PVFQKHCLFCMRCINSCPVNAIRYNKRKCWQYKCEPRGQSAESKPSALEEESK"
     gene            complement(921..>1870)
                     /locus_tag="VHR42_03910"
     CDS             complement(921..>1870)
                     /locus_tag="VHR42_03910"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HEX3026356.1"
                     /translation="SDRAYAIQTSKKENAWEGYPQRFAQVKEQFSKLPESTWRSNLYY
                     GWLWTLKGMAQPFGSGYPSFMTNQAWTDKSLSSALGSWSELRHDTILYGKQSGAEKGG
                     AEEPKVLGYVEPVVEVYDKLLWLTEYSEKNLAARGLLSGDDETGGIKGNVAYFENLLK
                     FLRDCSVKELKGETLTDDEYIRLYDYGENMEEMTASLSTDGLRWFEIQSETDKNMALI
                     ADFHTVAPNRYDNGGYLEAGVGPAHEIYVVVPIGGKLYLTRGAVFSYYEFESNDRLTD
                     EEWQKMLKGGKAPAQPSWTGSFISGGKGEIPKPQDPYNS"
BASE COUNT          423 a          490 c          434 g          523 t
ORIGIN      
        1 gctatttgct ttcttcttct aatgcagaag gcttgctttc cgctgattgt ccacgcggtt
       61 cgcatttgta ctgccagcac tttcgtttgt tgtagcggat ggcatttacc ggacaggagt
      121 taatacagcg catgcaaaac aggcagtgct tttgaaacac cgggtttccc tgaaccattc
      181 tgatgttttc cattgggcag cgcttcacac acagtccaca gtgaatgcag ctttcatccg
      241 cagaaaactt cattgcaaac acaggcataa gcttggaaaa ggttaccgcg accatataaa
      301 tcaaaggccg ctgccatgct ttcgtgtcct ccttggacat ttggcctttt aaaaaggcat
      361 ctaaaagggg ttccatccgt tttcggatat ccttttgatt ttccataagc gcagatggtt
      421 ccgtcggctt aaaagcttgg aaaatcatca tattattggc atagggaaag ctggcttcga
      481 cggtcagctg atgatttttc ttcctgagta gtttctcaaa acccgtaaaa tcggttttaa
      541 gcggcgatgc ctgtgtgcag aatgccatcg acggaatcag tttttcccgt ctttgcaagt
      601 tttgttttaa atagtcaagc ataagcatgg cagggtattc gtaatatttt gggcagccaa
      661 tgatcaggta atcataagaa atctttgtca gatcaatccg ctcttcaatc gaatgcataa
      721 ttaccgacag atttctttta atcaggcatg ccttgaaatc ttcagcaatt cttctggtgt
      781 ttccggtgcc gctaaagtag agaatcagta cagtcatttt aacctccaaa attgcacaag
      841 gaatttcatg ccggctgcga agttcagata gctgtctgga cttcgcagta ttcatctccc
      901 gcggattctg cagcggccaa ttaagaatta tacggatcct gaggctttgg tatttctccc
      961 tttcccccgc ttatgaaaga gccggtccaa gacggctggg ccggcgcttt gccgcctttc
     1021 agcattttct gccattcctc gtcagtcagc ctgtcgtttg attcaaactc gtaataactg
     1081 aaaaccgcgc ctctggtcag atagagctta ccgccgatcg gcacgaccac atagatttca
     1141 tgagcgggtc caacgcctgc ttccagatat ccgccgttgt cgtagcgatt gggggcgacc
     1201 gtgtggaaat ctgcaatcag cgccatattt ttgtccgtct cggactgaat ctcaaaccac
     1261 ctaaggccgt ccgttgacag ggaggccgtc atctcctcca tgttttcgcc gtaatcgtag
     1321 aggcggatgt attcgtcgtc ggtcagtgtt tcccccttga gttccttgac ggaacagtcc
     1381 ctcaaaaatt tcagcaggtt ctcaaaatac gccacgttac ctttaatgcc gccggtctcg
     1441 tcatcgccgg aaagaagccc tctggccgca aggtttttct cggaatattc cgtaagccac
     1501 aacagcttgt cgtatacttc caccacgggc tccacgtatc ccagaacctt aggctcctcg
     1561 gcgccacctt tttccgctcc gctctgcttg ccatacagga tggtgtcatg gcgaagctcc
     1621 gaccagcttc cgagcgcgct actcagggat ttatcggtcc acgcctgatt ggtcatgaag
     1681 gaggggtaac cgctcccgaa gggctgggcc atgcccttca aggtccacaa ccatccgtaa
     1741 tacagattgg agcgccaggt gctttcgggg agctttgaaa actgttcctt aacttgggca
     1801 aaccgctggg gatagccttc ccacgcgttt tcctttttgc ttgtctgaat ggcgtaggcc
     1861 cggtccgaac
//
LOCUS       DATALY010000132         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_374846,
            whole genome shotgun sequence.
ACCESSION   DATALY010000132 DATALY010000000
VERSION     DATALY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374846"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VHR42_03915"
     CDS             complement(<1..387)
                     /locus_tag="VHR42_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026357.1"
                     /translation="MKKKLNRTFAIALTLGLLLTGCSGQAASTSTSSQTVSSQTVSTQ
                     VSSGAPVDNGNIVVAESDDPQNINPLYVVDQTSFDIQQALYSPFFEIVKGKLFYGNGL
                     LESVTANSDSSVFTLKLKSNLKWHDGQ"
     gene            complement(402..1049)
                     /locus_tag="VHR42_03920"
     CDS             complement(402..1049)
                     /locus_tag="VHR42_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX3026358.1"
                     /translation="MKNPLYYQATEYDCGPTSMLNAISYLFEREEIPPIIIKTIMTYC
                     LDQYNEQGEICKGGTSCMAMKHLSDWLNQYHKINKFHISCEYLSGDEVELKRGGRIIR
                     SLENGGAVVLRVWYECGHYVLLTGADDRDVYLFDPYMRKVPFDTGGIEIVPNEPTRVN
                     RKVAYDILNAEGNGYYEMGPKSTREAVIVYNLDCKKGESVQSNLEALHSKISTLI"
     gene            complement(1082..1984)
                     /locus_tag="VHR42_03925"
     CDS             complement(1082..1984)
                     /locus_tag="VHR42_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX3026359.1"
                     /translation="MDVLLKRKYENMGLKFSIGSNLLFVVLEIWISYLSNSKAVLIDG
                     LFDGCEAILLLFSIRLMKYLYKPISEKRPIGYSNLEPFYMILKGLLFFVIAAMMALNG
                     ISSFFSGGYEVDMNIVFYFELFAGLYGIAAFLILKYLNSKARSKILDLEIKEWILDIV
                     TSFGTGIAFLTAFLIRFTAFSSLSRYFDQIITLAMVVYILPTPLKAIKSGFQELFFLS
                     PSEEMLQKVKSIGEDVTGTYGISKDQLEFDVVKTGRRLWISVYIEIKQNYIELSLLKD
                     IHSELEQKYAGLADLIDVDVIPDF"
     gene            complement(2174..3292)
                     /locus_tag="VHR42_03930"
     CDS             complement(2174..3292)
                     /locus_tag="VHR42_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3026360.1"
                     /translation="MKLVRLDRLTDKFIELTKIDSPSFQEREMADRLTRDLKSLGFSV
                     WEDDAAEKVNGNAGNLYAFLPGTEGSPLLFSGHMDTVNPALNKKAQLHPDGTITSSGD
                     TVLGADDVSGLAVILEAVASIQEDGLPHRPLEILFTIAEEPYDCGSEVFDYGKIKSKE
                     AYVLDLSGEIGGAAYAAPTILSFCVEISGKASHAGFAPEKGIHAIAAAADAISRLKMG
                     HLDEETTLNIGTIQGGTATNIIPDRCVVNGEIRSYSHSAAKAALQSVTDRFAESAASY
                     GAESRIKSREGCRAYEVPPDHPVIRRYQAACKKNGIEANLFKTFGGSDANNFSKYGIA
                     SIVIANAMFNVHSCSEYTVPSDMAKVAEVVRCMMLGGD"
     gene            complement(3320..4216)
                     /locus_tag="VHR42_03935"
     CDS             complement(3320..4216)
                     /locus_tag="VHR42_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026361.1"
                     /translation="MKKKTLRSESYGRIVWKRFLRHRVALVSLSVLTLLTLAAIFAPL
                     IAPHDPDAIAGMFGGAPNGSFWLGTDEIGRDVFSRLLYATQVSLLVGFMATVISTAVG
                     VALGLISGYFGGWIDMAVMRFTDMVMSFPYILLILVAAAIFKPGMWNIILILGFVDWP
                     GVARLVRGSTLSLRETNFVKSSVVAGMTKRHILFSEILPNTVAPILIYATLVLAVSML
                     DEAALSFLGMGVQPPTPSLGNMLNGAQSLTVLTTKPWLWVPPGFMIIVLVISINFIGD
                     ALRDAIDPTTVMSSGAAAPEKK"
     gene            complement(4244..>5134)
                     /locus_tag="VHR42_03940"
     CDS             complement(4244..>5134)
                     /locus_tag="VHR42_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026362.1"
                     /translation="TVIDYFLMNLAGSPLAMIQGAHVSQGALAARGAQLGLNKPVIVQ
                     YFTWLSQILTGNFGFSIKSNESVASMIASHLGPTLLLMGVSLLISMLIAVPAGVYSAT
                     HQNKAGDYSLMGLAFLGNSIPSFFLAMLLIYLFTIKLNLLPSGGMTTLGANLGTGDVV
                     IHMILPVIVLAVSMTGTNIRYIRSAVLEILQQDYLRTARAKGIGRFKVINKHALRNAM
                     VPVITVFGMEIPTLFGGAIIVEQVFSWPGLGLMTMTAIINRDYPVIMGVSLVAALVVL
                     IGNLLTDLLYAVVDPTIQYH"
BASE COUNT         1409 a         1351 c         1129 g         1245 t
ORIGIN      
        1 ctgaccgtca tgccatttca ggtttgattt cagcttcagt gtgaacacac tggaatcgct
       61 gtttgcggta acgctttcca acagaccgtt gccgtagaac agctttcctt ttacaatttc
      121 aaaaaacggc gaatacaaag cctgctgaat atcaaaactg gtctgatcaa caacataaag
      181 cggattgata ttttgcgggt catcggactc ggcaaccaca atattgccgt tgtccaccgg
      241 ggctcccgaa gaaacctgcg tcgatacagt ctgagaagat accgtctgcg aagatgtgga
      301 agtggaagct gcctgcccgg agcaccccgt taaaagcagt cccaaagtta aggcaattgc
      361 aaaagtgcgg ttcaatttct ttttcataat ttacaccttc cttatattaa tgtcgaaatt
      421 ttggaatgca aggcttccag attcgactga acggattcgc cttttttaca atcaaggtta
      481 taaacaataa cagcttctct tgtacttttc gggcccattt cgtaataccc gtttccctct
      541 gcgtttaaga tgtcataggc aactttccgg ttcacgcggg tcggttcgtt cgggacaatt
      601 tcaatgcctc ccgtatcaaa cggcactttt ctcatataag ggtcgaacag gtaaacgtct
      661 ctgtcgtcag cacccgttag taacacatag tgcccgcatt cataccacac gcgcagcacg
      721 acagctcccc cgttttccaa agaccggatg attcttcctc cgcgttttaa ctcgacctcg
      781 tcgccggaaa gatattcaca gcttatgtga aacttattga ttttatgata ttgattcagc
      841 caatcgctca gatgtttcat cgccatacag gaggtaccgc ctttgcagat ttccccctgc
      901 tcgttatatt ggtccagaca atacgtcata attgttttga tgataatcgg cggtatctct
      961 tcccgttcaa ataagtagct gattgcatta agcatggaag tagggccgca atcgtattcc
     1021 gttgcctgat aatacaaagg atttttcatt ttcaacacct tgattgtatg agatagaatg
     1081 tttaaaagtc cgggatcaca tccacatcga tcaggtccgc aagccccgcg tatttttgct
     1141 ccaattcgga atggatgtct tttaataaac tcaattcaat ataattttgc ttaatttcaa
     1201 tatacacact gatccacagg cgccttcccg ttttcaccac atcaaactcg agctgatctt
     1261 tcgaaattcc gtaagttccg gtaacatcct ccccgatact ttttaccttt tgcagcattt
     1321 cctcgctcgg gctcagaaaa aacagttcct gaaagcctga cttaattgct ttcaggggag
     1381 tcgggagaat ataaacgacc atagccagcg ttatgatctg atcgaaatat ctggacagag
     1441 agctgaaagc cgtgaaacgg atcaaaaatg ccgtgagaaa agcaattccg gttccgaagc
     1501 ttgttacgat atcaagaatc cactctttga tttcgagatc cagaatcttt gatctggctt
     1561 tggaattcag atattttaag atcaggaagg cagcgatccc gtaaaggccg gcaaaaagct
     1621 caaaatagaa aacgatattc atatcgactt catatccacc ggaaaagaaa gacgatatgc
     1681 cgttcagtgc catcatggcc gcaattacaa aaaacaaaag tccttttaag atcatataaa
     1741 acggctccag attggaataa ccaatgggcc tcttttcact gatcggctta tacagatact
     1801 tcatcagccg gatggaaaaa agcagaagaa ttgcttcgca tccgtcaaag agaccgtcga
     1861 tcagcaccgc ttttgaattg gataaatagg aaatccagat ttcaagcaca acaaataaaa
     1921 gatttgaacc gatagaaaat tttaaaccca tgttctcgta ttttcgcttt aataaaacat
     1981 ccataaaata gcctaattga agggctgaat taggggagat ttttccccgc tctccttaat
     2041 aaaaacgtat gcggctctcc agccctttgt gtcaatacag gcaccagttt cctccaaaat
     2101 caatcatcta ccgtggaata tacaagccga tttccacggc ggctttctca aacgcctgcc
     2161 tggaattgaa gaatcaatcc cctccgagca tcatgcagcg caccacctcg gcaacttttg
     2221 ccatatccga aggaaccgta tattcactgc aggagtgaac gttaaacatc gcgtttgcaa
     2281 tgacaatgct cgcaattccg tactttgaaa aattgttcgc gtcactgcct ccgaacgtct
     2341 tgaaaaggtt tgcctcaatc ccattttttt tgcaagccgc ctgatatcgc cggatcaccg
     2401 gatgatccgg cggcacttca taggcccggc atccttcccg gctcttgatc cgggactccg
     2461 caccgtaaga cgcggcagac tccgcaaacc ggtcggtcac agactgcagc gcagctttgg
     2521 cggccgaatg ggaataagag cggatttccc cgttgacgac gcagcggtca gggataatat
     2581 tcgttgccgt tccgccttga attgttccga tattcaaggt ggtttcttca tcaagatggc
     2641 ccatcttcaa acggctgatt gcgtccgccg cggccgcaat cgcatgaatt cccttctccg
     2701 gggcaaatcc ggcgtgggaa gccttgcctg aaatttcgac acaaaaagaa aggatggtcg
     2761 gcgcggcata agccgcccct ccgatctctc cggaaagatc cagcacatac gcctcttttg
     2821 acttgatttt cccatagtca aaaacttcgc tgccgcagtc gtacggctcc tctgctatcg
     2881 taaaaaggat ttccagcggc cggtgaggaa ggccgtcttc ctgaatggag gcaaccgctt
     2941 ccagaatcac cgcaagaccg gaaacatcgt cggctccaag caccgtatct ccggaacttg
     3001 tgatcgtccc atccggatga agctgtgctt ttttattcaa agcgggattg accgtgtcca
     3061 tatggcccga aaaaagcagc ggtgagcctt ctgtgccggg aaggaaggca tagagatttc
     3121 ccgcgtttcc attcactttc tcagccgcgt catcttccca gacggaaaac ccaaggcttt
     3181 tcaggtcacg tgtcagccgg tcggccattt cccgctcctg aaacgacgga ctgtcgatct
     3241 tcgtcaactc tataaatttg tcggttaagc gatccagacg aaccaatttc atttacccct
     3301 tttcccgaaa actcaaaact tattttttct caggcgctgc cgctccggag ctcatcaccg
     3361 ttgtcggatc aatcgcatcc cgcagggcgt cgccgataaa gttgatcgat atgacaagaa
     3421 caataatcat gaaaccaggc ggcacccaaa gccacggttt cgtcgtaaga accgtgagcg
     3481 actgcgcacc gttgagcata ttcccgaggc tgggtgtcgg cggctgaacg cccattccga
     3541 gaaagctcag cgctgcttca tccagcatgg aaaccgcaag caccagcgta gcgtaaatta
     3601 gaataggcgc cacggtgttg ggaagaattt cagaaaacag aatgtgccgt ttcgtcattc
     3661 ccgcgaccac actgcttttt acaaaattcg tctctcgcag ggaaagcgtg ctgcctcgaa
     3721 ccagacgcgc aactccgggc caatccacaa aaccgagaat caagatgata ttccacatgc
     3781 cgggcttaaa aattgcggcg gccacaagaa tcagcagaat atagggaaaa gacattacca
     3841 tatcggtgaa gcgcatcacc gccatatcaa tccagccgcc aaagtagccg gagatcaatc
     3901 caagagcaac gccaaccgct gttgaaatca ccgtagccat aaagcctacc agcagcgaaa
     3961 cctgcgttgc atacaaaagg cggctgaaaa catcgcgtcc aatctcatcg gtacccaacc
     4021 agaacgatcc gttcggcgca ccgccaaaca ttccagcaat cgcatcggga tcatgcggtg
     4081 cgatcagcgg agcgaatatc gccgccaatg tcaggagggt caataccgac aggctcacaa
     4141 gagctaccct atgcctgaga aagcgtttcc agacgatccg tccgtaactt tcacttctta
     4201 acgtcttttt cttcaaaaag ctcaaagtct tctctccctc ctttcaatga tattggatgg
     4261 tcggatcgac gaccgcgtag agaagatccg tcagaagatt gccaatcaga acaacaagcg
     4321 cagcaacaag gcttaccccc ataatgaccg gatagtcgcg gttaataatc gcggtcatcg
     4381 tcatcagccc aagtcccggc catgaaaaca cctgctccac gataatggct ccgccgaaaa
     4441 gagtgggaat ttccattccg aacacggtga tcaccggcac catggcattg cgcagtgcat
     4501 gcttattgat cactttaaac cggccgatcc ctttggcacg cgctgtgcga agataatcct
     4561 gctgcaatat ttccagcaca gcactgcgga tataccggat gttcgtaccc gtcatggaaa
     4621 ccgccagcac gataaccggc aaaatcatgt gaataacgac atctccggtc cccagatttg
     4681 ctccgagcgt tgtcattccg ccggacggaa gcaaattcag ttttatggtg aaaagataaa
     4741 tcaggagcat tgcgagaaaa aaacttggga tgctgtttcc aagaaacgcc aaacccatca
     4801 gggaataatc gccggcttta ttctgatgtg tggcgctgta gacaccggcc ggtaccgcaa
     4861 tcagcatgct gatcaaaagg gaaactccca ttaaaagaag ggtcggaccg agatgtgacg
     4921 caatcatgga agctacggat tcattgcttt tgatcgaaaa cccgaaattg ccggtaagaa
     4981 tctgggaaag ccaagtaaaa tactgtacaa tcaccggctt gttcaggcca agctgggcac
     5041 ctcttgcggc aagcgccccc tgtgaaacgt gagcaccctg tatcatggca agcgggcttc
     5101 cagcaaggtt cattaggaaa tagtcgatga cggt
//
LOCUS       DATALY010000133        10454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_29823,
            whole genome shotgun sequence.
ACCESSION   DATALY010000133 DATALY010000000
VERSION     DATALY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29823"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..565
                     /locus_tag="VHR42_03945"
     CDS             113..565
                     /locus_tag="VHR42_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026363.1"
                     /translation="MNGQTVQIPASYVNQAENYFASHHISDDQAEYILGQVEAAKEAV
                     RESGVTDLKKLDKSTKNRIMAAAQAAADEANLKLSVGSNKDIKIVDSSGNVTFTGENV
                     IKTTGAAVPWERNAAFCAAFISSDLLLCSFLIFKYRLLEKKDESKTKS"
     gene            543..1202
                     /locus_tag="VHR42_03950"
     CDS             543..1202
                     /locus_tag="VHR42_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HEX3026364.1"
                     /translation="MNQKRSHKAGFILIPIAFLLIGYLVLFLLVEPFLSAYQSTVSLL
                     FFNSDQQSSQKLSNIFTAPPKKETAEVKLSDITFPQYGTRFGRLTISDASICADLYFG
                     DETDELKKGVGLYSGSFIPGYGRTILIAGHNHTYFHTLGNVKPGDTVLIETNYGKYTY
                     QITKTQIQSFDNPSAYDLNQNEENLILYTCYPFNDLGLTPKRFFVYAKKLNGPTIDKQ
                     G"
     gene            1209..2051
                     /locus_tag="VHR42_03955"
     CDS             1209..2051
                     /locus_tag="VHR42_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026365.1"
                     /translation="MKKSTAEKAVSVLISFLISLFLFLFACVLIAQGTLLNPDYLRKQ
                     LYESHYYENVLEEVENEFSSYASASGFDQKLLNSALDINDLQLVANQSLNRIYGEKAE
                     NISNANFQNKMNRILTQNAESRGVTLTEDNKKSLLLLAQTCSDTYLKDISFPYTEELG
                     EAIGKIKTVLRLPSVLLPVLVVILAAILFRITPWRHRALRACIYAVSGTALMLAAFPA
                     YLYFSGRMGQVAILSKSLYVLTVSCANGILILFLEGAAVTVGIAAVLCLLYYRSRERV
                     SKVY"
     gene            complement(2088..2837)
                     /locus_tag="VHR42_03960"
     CDS             complement(2088..2837)
                     /locus_tag="VHR42_03960"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX3026366.1"
                     /translation="MTPMLPSRPFIDFHTHILPAIDDGSPDLGTSADMLSSLKWQGVG
                     TVVLTPHFYTGHESISSFLRRREAAWEALVPILGRAGMTAVLACEMHFSDYLFNSADL
                     SALCIGGKFLLTELPYSFASSETQISKIGRLISSYNVTPVLAHIERYPELLKKRGRLD
                     QLLEMGCRTQINLSSLLNGSMRLRKTLFSLIQSSRIHVVGTDCHNMKSRPPFFEPGIQ
                     MIAKKLGENAVRLLAENAESMLLQNESSRLA"
     gene            complement(2803..3540)
                     /locus_tag="VHR42_03965"
     CDS             complement(2803..3540)
                     /locus_tag="VHR42_03965"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX3026367.1"
                     /translation="MKRRNGTGQPLAGDGICPERMFEITEAYKLIRTNIMFSILKDGC
                     KKIVISSSQSREGKTTTAINVAVSLSQTDAKVLLIDADLRKPRVGQFFDYKESPGLTN
                     YLGKLVSLPESLHQTEFSNLSVLCSGVNVPNPSELLASEEMASFLAEMESCYDYIVID
                     TPPLNVVVDALPLIKLSDGVILVVRERYSTFPQLNQSIRNVELIGGKILGMVLNDSKS
                     RGKGLEKYKYGYGSAYYDTYAAQPTVH"
     gene            complement(3537..4259)
                     /locus_tag="VHR42_03970"
     CDS             complement(3537..4259)
                     /locus_tag="VHR42_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX3026368.1"
                     /translation="MEISLKDLFLLLKKNFIFLICCSLAGCAVAFLFFRFMVKPTYVS
                     NVELYVYTKESSATAENYNSLNDLNYAQKIVNTYIEMLRTNSFMKEVKENTNLNYSID
                     DLKKMVKFTVLNDTEVFQVSVSSHRPEDAKQIADTITNLAPQTIRSIKESALLKVVDS
                     ATFPASPSSPDVALCSVVGFLCGAAVSIVYLLLKEMLDVRVKREEDLAAKYNIPLLGS
                     IPSFETQTGKTLIFAEKEGAKQ"
     gene            4475..5191
                     /locus_tag="VHR42_03975"
     CDS             4475..5191
                     /locus_tag="VHR42_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX3026369.1"
                     /translation="MKDNVVYNPAVSVCSGENERIELYSIIANPPIETKKRRLIYHGI
                     KRIFDVILSAFAIAILFLLLLIVSVAIRLDSKGPAIYTQKRAGKKGKSFTIYKFRSMC
                     EDADQLRETLRDQNECDGPVFKIVSDPRVTRVGKFLRKTSLDELPQLFNILKGDMSIV
                     GPRPPLLSEVEQYTPYQMHRLDVKPGLTCYWQISGRSDIGFDKWVELDMKYIEERSLW
                     TDLKIILKTVPAVLLERGAY"
     gene            5929..7605
                     /locus_tag="VHR42_03980"
     CDS             5929..7605
                     /locus_tag="VHR42_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX3026370.1"
                     /translation="MILSISALVLILTTWVCTVEWMQRTQIGYDIYNSIVTGYNLKSD
                     IAPSSGDLEKAYSVALEYISEPNASRQAEQLTEFNTVKNSYEERYAYWQKSNLMAYNA
                     SVAGAWKAQHESAEKFLDLFQNSVVPAVKNKTALQPVRSDLIAAYQSYNALAAKTLQL
                     TQQQQQKDTAAATYYDSQTTVLIIALILLCLAILIAISFAISHSLVRHVRYISSVSNK
                     IAQGDLTVQVDEKQMTRDEIGRLCETTARTLSRLNQYIHYINEISDVLKTMANGDMRI
                     QLKYDYSGEFLPVKTALQGISTSLNQTLTVISDSADQVNSGSSQVSDGAQALASGATQ
                     QSASVAELNGSVQNIARQAGSNAENVEQAAGYVQQTNREIQNGSERMAQLTRSMEDIA
                     EASDEISKISKVVEDIAFQTNILALNAAIEAARAGEAGKGFSVVADEVRNLAAKSEQA
                     AKQTGTLIQRTIGTIGEGSKLTNEVAQIMNGIQEKSNLVNQSIEQIKAVSAEQAANTG
                     KIMQGLSRISEVVQTNAATAEESSAASEELSAQAFKLREEIGKFQLVSEN"
     gene            complement(7695..8687)
                     /locus_tag="VHR42_03985"
     CDS             complement(7695..8687)
                     /locus_tag="VHR42_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3026371.1"
                     /translation="MKTKKHLSIRDIAEKTGFSTATVSRVINNHGGYSEATKKAVNEA
                     IQQSGYTTGTNTSRRIGILVPDLANEWFAQLCVALEKYLFEYGYSAFICNANEDSKRE
                     NYYFMEFSNCKVAAMICFVGSKALGTLAAGANFPVIFIDRVPIDTEQFICLESDNYLG
                     GYMATELLIHKGCKRIMLLGWERPLSVNLERQQGYLDALKDHDIAVDKELIVELNDES
                     DHYEGSKHLVSYLANKKLNFDGVFATNDLRAYGALQALQQNGVDVPGQVKIVGYDDSA
                     VSQRCYPALTTVHQDCNQMAKKAVEILMKRIDDPTSGGSEHIVLPVSVIERGTT"
     gene            9179..>10454
                     /locus_tag="VHR42_03990"
     CDS             9179..>10454
                     /locus_tag="VHR42_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3026372.1"
                     /translation="MEPLIKMVGITKVFPGVKALDNISFDIMPGEVHVLLGENGAGKS
                     TLMKILSGVYQPTSGKIIVGGKEYDSLTTKDSYDEGIAIIYQELSVINELSILENLFV
                     GKLPTVKKCGIPVVDYKTMTEKATAVLQKVGVHRPLKTFVEDLTMPEKQQVEIAKALV
                     SDAKIIIMDEPTTSLTTAETNNLFELIRKLKSENKGIVYISHKMDEIKKIGDRVTVLK
                     DGTYVGTRNADEVTVDDMVTMMVGREIKGTYFGDPDVDLMKQPVIFEAKDITRSDDKC
                     RNVSFQLHKGEILGFAGLVGAGRTETMEAIFGAHPKSKGQVFLNGRELHINSPYSAIK
                     QGLGMVTENRRETGIIDNFNIKQNISMGPYIKESSAGGIGGLLDGKRETRWAEEQKTN
                     LRVKCTGIDQMIVDLSGGNQQKVILGKWLAANT"
BASE COUNT         2800 a         2406 c         2616 g         2632 t
ORIGIN      
        1 tccaacggta aaagatgcac tgaccgcttg ctggatttca ttattataat ttgctgtgcc
       61 cgccgcggcc aatacggacg caattctgga ttccctgcgg tcgggtattt cagtgaatgg
      121 gcagacggtt caaattccgg caagctatgt caatcaggcg gaaaactatt ttgcctcgca
      181 tcatatatcg gacgatcagg cggagtacat tctagggcag gtagaagcgg cgaaagaagc
      241 ggttcgggaa agcggcgtca cggacttgaa aaagttagac aaaagtacaa agaatcggat
      301 tatggcagcg gctcaggctg cggccgacga ggcgaatctg aagctttccg tcggctctaa
      361 taaagatata aaaatcgtgg attccagcgg aaatgtgacg tttacagggg aaaatgtgat
      421 taagacgacg ggcgctgccg ttccgtggga gaggaatgcg gcattttgcg cggcgtttat
      481 ttcgtctgat ttgctcctgt gttccttttt gattttcaaa tacaggctgt tggagaaaaa
      541 ggatgaatca aaaacgaagt cataaagcag gctttatctt gataccgatc gcttttttgc
      601 tgatcggtta tcttgttttg tttctgttgg ttgaaccgtt cctttcagcc taccaatcga
      661 ccgtaagcct gctctttttt aattccgatc agcagagcag ccaaaagctg agcaacattt
      721 ttacagcgcc gccgaaaaaa gagacggcgg aggtaaaact gagcgacatt acgttcccac
      781 agtatgggac acgcttcggc cgccttacga tttcagacgc ttccatctgt gcggacctct
      841 atttcgggga tgaaaccgat gagctgaaaa aaggggtcgg gctatacagc ggcagtttca
      901 tcccgggcta cgggcgaacg attttaattg cggggcataa ccatacttat tttcatacgc
      961 tggggaatgt gaaaccgggg gatacggttc tgattgaaac caattacggg aagtacacct
     1021 atcagattac caaaacgcag atccaatcgt ttgataatcc gtcggcgtat gatttgaatc
     1081 agaacgagga gaatctgatt ctttacacct gctatccgtt taatgatctt ggattgacac
     1141 ccaagcgatt ttttgtttat gcgaaaaaat taaacggccc aacgatagac aaacaaggat
     1201 gaataatgat gaaaaaatcg accgccgaaa aagcggtatc cgttttaatt tcttttctga
     1261 tttccctgtt tctgtttctg tttgcctgtg ttctgattgc gcaggggacc cttttaaatc
     1321 cggattattt gcggaagcag ttatacgaaa gccactatta tgaaaatgtg ctggaggaag
     1381 tggagaatga gttttcatcc tacgcttcag caagcggttt tgaccaaaag ctgctgaatt
     1441 cggcgttgga tatcaacgac cttcagttgg ttgccaatca gtcgctgaac cgcatttatg
     1501 gggaaaaagc ggaaaatatt tcaaacgcaa attttcagaa caagatgaat cggattctga
     1561 cccaaaatgc ggaaagccgc ggggtcacat taacagaaga caacaaaaaa agtctgctcc
     1621 ttcttgctca aacctgttcc gatacctatt taaaggatat cagttttccc tatacggagg
     1681 aattggggga agcaatcggg aaaataaaga ccgttctgag actcccgagc gttctgctgc
     1741 cggtactggt tgtgatcctc gcggcaatct tgttccggat taccccttgg cggcaccgag
     1801 cactgcgggc ctgcatttat gcggtctcag gtaccgccct gatgcttgct gcttttcccg
     1861 cttatcttta cttttccggg cgaatgggac aggttgcgat cctcagcaag tctctttatg
     1921 tattaaccgt ttcctgtgca aacgggattt tgatcctgtt cctggaaggc gccgctgtta
     1981 cagtgggaat cgccgcggtc ctgtgtctgt tgtattaccg ctcccgggaa cgggtgagca
     2041 aagtctatta atttaaagag gcagtcaaaa aagctcccgc tcgctgttca cgcgagtcga
     2101 gagctttcgt tttgcaggag catggattcc gcgttttcgg ccagaagacg gacggcattc
     2161 tccccgagct ttttcgcgat catctggatt ccgggttcga agaagggagg ccgggatttc
     2221 atattgtgac agtcggtgcc gacaacgtga atacggctgc tttgaatcaa agagaacaat
     2281 gttttgcgca gacgcatgct gccgtttagc agagagctga gattgatctg tgtgcggcag
     2341 cccatttcaa gcaactgatc cagacgaccg cgctttttca gcagttccgg ataccgttcg
     2401 atatgagcga gcaccggcgt aacgttatag ctggaaatca gccggccgat tttgctgatc
     2461 tgggtttcgg aagaggcaaa ggaataaggc agctccgtca gcagaaattt tcccccgata
     2521 cacagagcgg aaagatcggc agaattgaac aggtaatcgg aaaagtgcat ttcgcaggca
     2581 agaacagccg tcattccggc ccgtcccaga atgggaacaa gcgcttccca cgcggcttca
     2641 cggcgccgta gaaacgagga aatcgattcg tggccggtat aaaaatgggg cgtgagaacc
     2701 acggtgccga caccctgcca tttcagactg gagagcatgt ccgcagacgt gcctaggtcc
     2761 ggagaaccgt catcgatagc cggcagaata tgcgtgtgaa aatcaatgaa cggtcggctg
     2821 ggcagcatag gtgtcataat aagcggatcc atagccgtac ttgtattttt ccaagccctt
     2881 gcccctggat ttcgaatcat ttaaaaccat tcccaatatt tttcccccga tcagttcgac
     2941 gttccgaatc gactgattca gctggggaaa cgttgaatat cgttctcgga ccacgaggat
     3001 gacgccgtcg ctcagtttaa tcagggggag cgcatccaca acgacgttga gcgggggcgt
     3061 atcaatgacg atatagtcat agcagctttc catttccgcc agaaaggaag ccatttcttc
     3121 acttgccaaa agttccgatg ggtttggaac gttcacaccg gagcataaaa cggaaaggtt
     3181 gctgaattcc gtttgatgga gggactccgg caggcttacc agcttgccga gataattcgt
     3241 cagccccggc gattctttat aatcaaagaa ctggcctacc cgcggtttgc gcaggtccgc
     3301 gtcaatcagc agaactttgg cgtccgtctg agagagggaa acggcgacgt taatggcggt
     3361 cgtggttttt ccttcccggg actgtgagct tgaaatcacg atttttttac agccgtcttt
     3421 caggatggag aacataatgt tggttcggat cagcttatag gcttcggtga tttcaaacat
     3481 ccgctcaggg cagattccat cgccggcaag cggctgcccg gtgccatttc tgcgtttcat
     3541 tgttttgccc cttccttttc cgcaaaaata agagtcttcc cggtttgcgt ttcaaaagag
     3601 ggaatggacc caagaagcgg aatgttgtat tttgccgcca gatcttcttc ccgcttaacg
     3661 cggacatcca gcatttcttt tagaagcaga taaacgatgg ataccgccgc gccgcataaa
     3721 aagccgacaa cactgcacag ggcaacatcg ggggaagaag ggcttgcagg aaacgtagcg
     3781 gaatcaacca cttttaaaag ggcagattct tttatggaac ggatcgtctg aggcgccaga
     3841 ttggttatgg tatcggcaat ttgtttggca tcctccggcc tgtgagaaga gacggatacc
     3901 tgaaaaacct cagtatcgtt taaaacggta aatttgacca tctttttcag gtcgtcgatg
     3961 gaataattca gattggtgtt ctctttgacc tctttcataa aactgttggt gcggagcatc
     4021 tcgatgtagg tattgacgat tttctgtgca taattcaggt cgttcagact gttgtaattt
     4081 tcggcagtcg cagaactttc tttggtgtag acgtacagtt caacgttgga aacgtaggtg
     4141 ggctttacca taaaccggaa gaacagaaaa gcaacagcgc acccggcgag ggagcagcaa
     4201 ataagaaaga taaagttttt tttcagcagc aaaaaaagat ctttcaacga aatttccatg
     4261 agacgttttt ctcctaacgc aaatgatgat cccattataa taccaaaatt ttccaaatac
     4321 aatcatattt aggagaagaa ggggataaat tttttatgaa gttgacggag aaaaactgac
     4381 aattttccgt tgaactatta attccaatca attaaaatgg tcacatatta acgaacttga
     4441 aaaaatagca taataaatga gagctggtgg cttcatgaaa gataatgttg tttataaccc
     4501 ggcggtgtcc gtttgctcgg gggaaaatga acggatcgag ctgtattcca taattgcgaa
     4561 tccaccgatt gaaacaaaaa aaaggcgtct gatttatcac ggaatcaagc gcattttcga
     4621 tgtgatttta tctgcctttg caatcgcgat tctgttcctt ttgcttttga ttgtatccgt
     4681 tgcgatccgg ctggattcca aaggaccggc tatttacacg cagaaacggg ccggcaaaaa
     4741 gggaaaatcc tttaccatct ataaattccg aagcatgtgt gaggatgccg atcagttgcg
     4801 tgagactttg cgggaccaaa acgagtgcga cgggccggtt tttaaaatcg tgagcgatcc
     4861 ccgcgtgacc agagtgggga agttcctgag gaaaaccagc ttggacgaat tgccccagct
     4921 tttcaatatc ttaaaaggcg acatgagcat cgtcggcccg cggcctccgc tcttaagcga
     4981 ggtggaacag tatacgccgt atcagatgca ccgcctggat gttaaacccg gcctgacctg
     5041 ctactggcag atcagcggaa gaagcgatat cggttttgac aaatgggttg agctggatat
     5101 gaaatacata gaggaacgca gtttatggac ggatttaaaa atcatcctga aaaccgtacc
     5161 ggcagtgctg cttgagcggg gagcgtattg atccgggtta tttcttcttt ttccccaacc
     5221 gaaaacagca cccgaggaac agaccggcat aaaagcaaat ggcgcctgtg attacatagc
     5281 ccataatgcg gcacgacctc ccactttatt cttaattctt atatatcgga actgattcgc
     5341 agaagtttaa ttgctattat ataaaataca aaatttattt ccaatagaaa gtattattcc
     5401 gaaactccct tattattcga attgaaaaca ataatttcag ttcggccttg ggggttatga
     5461 tttgtaaaat cgaccactga ccgccgatgt atgcgaagcg accgacgttt tacgatatag
     5521 atacttaacg gtattgaaat cggatttttg ttatagccgc aagatcgaag ccacgagagg
     5581 aaatgttttc gcggcttttt ttgttgccca taggcagaaa tctccgcacc aactgaaatt
     5641 cgctttaaat gattattatt taaagtaaat atattgtaaa acaaaccgat aaatattact
     5701 atagaatata agattgttaa ataaacaacg gcaaattccc tgcttcggct gaaaatttat
     5761 tttactgtgt cgaaaacgct tggaatacgg caggtatgcc ggagattttt tctgccacaa
     5821 ttaattttat ctctaaattc aatcaagtca gacaaaaaat tgataaaaaa gggaaaggtt
     5881 ctgaatcagt tggactggct gaaaaatgtt aaaatcagag gaaaattatt gattctatcg
     5941 atctcggctt tggttcttat ccttacaacc tgggtgtgca ccgttgaatg gatgcagcga
     6001 actcaaatcg gctatgatat ttataacagc attgtcactg gctataatct gaagtcagat
     6061 atcgcccctt cctccggtga tttggaaaag gcgtattccg tcgcgctgga atatatttcg
     6121 gagccgaatg cttccaggca ggcagagcag cttacggaat tcaatacggt taagaacagt
     6181 tatgaagaac gatatgccta ttggcagaaa tccaatctga tggcttacaa tgcgtctgtt
     6241 gccggggcgt ggaaggcaca gcatgaaagt gcggaaaaat ttctggacct tttccagaat
     6301 tccgttgttc cggctgtcaa gaataaaaca gcgctgcagc cggtgagatc cgatttgatt
     6361 gcagcgtatc agtcctataa cgctcttgcg gccaaaacgt tgcagcttac gcagcagcag
     6421 cagcagaaag atacggctgc cgccacctat tacgatagcc agaccacggt gctgattatc
     6481 gctttgatcc ttctttgcct cgcaatctta attgccatca gctttgcgat ttcacactct
     6541 cttgtccgcc atgttcggta tatttcttct gtaagcaata aaattgccca gggagacctt
     6601 accgtacagg tggatgaaaa acaaatgacg cgcgacgaga tcggcaggct ttgcgaaaca
     6661 accgccagaa cgctttcccg gctgaatcaa tacattcatt atatcaacga aatcagcgat
     6721 gtgcttaaaa ccatggcaaa cggtgatatg cgcatccagc tgaaatatga ttattccggt
     6781 gagtttttgc cggtaaaaac tgcgctacag ggtatttcca cgtcgttgaa ccagacgctt
     6841 acggttatca gcgattccgc agaccaggtg aactccggtt caagccaggt ttcggacggc
     6901 gcacaggcgt tggcttccgg cgcaacgcag cagtcggcat ccgttgcgga gctgaacggt
     6961 tccgttcaga atattgctcg gcaggccggc agcaatgcgg aaaatgttga gcaggcggca
     7021 ggctatgtac agcagacaaa ccgcgaaatt cagaacggca gcgaacgaat ggcacagctt
     7081 acccgatcga tggaagacat tgccgaagcg tccgatgaaa tcagcaagat cagcaaagtg
     7141 gtagaggaca tcgctttcca gacgaatatt cttgcgttga acgcggccat cgaagcagcg
     7201 cgcgctggtg aagcgggaaa aggattttcc gttgttgcgg atgaggtgcg aaacctcgcc
     7261 gcaaaatcgg aacaggcagc gaaacagaca ggaacactga ttcagcgtac gatcggcaca
     7321 attggagaag gttccaaatt gaccaacgaa gttgcgcaga ttatgaatgg aattcaggaa
     7381 aaatcaaatc ttgtcaacca atcgattgaa cagattaagg ccgtctccgc ggaacaggct
     7441 gcaaacaccg ggaagattat gcaggggctt tcccggattt ccgaagtggt gcagacgaac
     7501 gcggctaccg cggaagaaag ctctgccgcg agcgaagaac tttccgctca ggcgtttaag
     7561 ctgcgggagg agatcggaaa attccagctg gtatcggaaa actgatcttt tccgcgcgaa
     7621 tacatgtgag ccgaaaaaga acccgaaaag ccatcatcca ttggcttttc gggttctttt
     7681 gtgtatgtat cggctcaggt tgtcccccgc tcaatgacgg ataccggcag gacgatgtgt
     7741 tcggagccgc cgcttgtggg gtcatcaatc cgcttcatca aaatttcgac ggccttcttc
     7801 gccatctggt tgcagtcctg atggacggtc gtcagcgcgg gatagcagcg ctggctgact
     7861 gcgctgtcgt cataccccac gatttttacc tgcccgggca cgtcgacgcc gttttgctgc
     7921 agtgcctgca gcgcgccgta cgcgcgcaaa tcgttggtgg cgaaaacgcc gtcgaaattc
     7981 agctttttat ttgccagata ggaaaccaga tgcttcgaac cttcataatg gtcgctttcg
     8041 tcgttgagct ccacgatgag ctccttgtct accgcgatgt cgtggtcctt cagcgcgtcc
     8101 aggtacccct gctgccgctc cagattcacg gaaagcggcc gctcccagcc gagaagcatg
     8161 atccgtttac agcctttgtg gattaacagc tcggtcgcca tataaccgcc cagatagttg
     8221 tcggattcca gacagataaa ctgctccgta tcaatcggga cacggtcgat gaaaataacc
     8281 ggaaaatttg cgccggcggc gagagtgccg agcgctttgc tgccgacaaa gcagatcatc
     8341 gccgcaacct tgcagttgga gaattccatg aaatagtaat tttcccgctt gctgtcttca
     8401 tttgcgttgc agataaacgc ggaatagcca tattcaaaca ggtatttttc cagggccaca
     8461 cagagctggg cgaaccattc gttcgcgagg tccggaacca gaatgccgat tcgcctgctg
     8521 gtattcgttc cggtggtgta accggattgc tgaatcgcct cattgacggc cttttttgtc
     8581 gcctcggaat aaccgccgtg gttattgatt acgcgtgaaa cagtcgcggt cgaaaatccg
     8641 gttttttccg caatgtcccg aatggaaaga tgtttcttcg ttttcatgaa aactctcact
     8701 cgtttacatt tatattttta caggttccgc tgccggatga cgggtgcagc ggaacttttt
     8761 tacgctgaga caacgtaacg tttcgctcac taagtcagta aaccgcaggc cttcctgcgg
     8821 agcaagtccc tgcaatattc cgtctgctcg ttatatctct tattttacag ggttcccgtt
     8881 cttctgtaaa gagaaaatga aggaattttt atgccaactg gagaagaatg ttcctctatt
     8941 ataacgtaac gttaacatga ttgcaagtga atattaacaa atttaataaa atccattgac
     9001 taaccatgtc acgtttgcta cactgtaaac gcttcaatga tgtgttactt aattttttag
     9061 aaatgttact cttttatagt tcgtaaattc aagtattgtt tcaatgatct gttcccaata
     9121 ggaatgaaac ttatttctct tgggctatcg ataagcatgt gacagaaaga aggattttat
     9181 ggaacctttg ataaaaatgg ttggcataac gaaggtcttt cccggcgtga aagcgctgga
     9241 caacatctcc tttgacatta tgcccggcga ggtccacgtt ctgctgggag agaacggcgc
     9301 aggcaagtcc actctgatga aaatcctgag cggcgtttat cagccgactt ccggcaaaat
     9361 catcgtaggc ggcaaggagt atgacagcct gactacgaag gattcctatg atgagggcat
     9421 cgctattatc tatcaggaat tgagcgtgat caacgagctg tccatccttg agaatctttt
     9481 cgtcggcaag ctgcccaccg tgaaaaagtg cggtattccg gttgtggatt acaaaaccat
     9541 gacggaaaaa gcgaccgcgg ttctccagaa ggtcggcgtg caccgcccac tgaaaacctt
     9601 tgtggaagac ctgaccatgc cggaaaagca gcaggtcgaa atcgccaagg ctctggtttc
     9661 cgacgcgaag atcatcatca tggatgagcc gaccacgtcg ctgaccaccg cggaaacgaa
     9721 caatctcttc gaattgatcc gtaagctgaa aagcgagaat aaaggcatcg tctatatctc
     9781 gcataagatg gacgaaatta agaaaatcgg cgaccgtgtc accgtactga aggacggcac
     9841 ttacgtcggc acccggaacg cggatgaagt tacggtcgat gacatggtca ccatgatggt
     9901 cgggcgcgaa atcaagggaa cctatttcgg cgaccccgat gtcgacctta tgaaacagcc
     9961 cgttattttt gaagcgaaag atattacccg ttccgacgac aaatgcagaa acgtctcctt
    10021 ccagctccac aagggcgaaa ttctgggctt tgccggtctg gtaggcgcgg gacgcacgga
    10081 aacgatggaa gccatcttcg gcgcgcatcc caaaagcaaa ggtcaggttt tcctgaacgg
    10141 ccgggagctg cacatcaaca gtccctactc cgccattaag cagggtctcg gcatggtaac
    10201 tgaaaaccgg cgcgaaacgg gcatcatcga caatttcaac atcaagcaga atatctcgat
    10261 gggtccatac atcaaggagt cgagtgccgg cggcatcggc ggccttctcg acggcaagcg
    10321 ggagacccgg tgggccgaag agcagaagac gaatctgcgc gttaaatgca ccggtatcga
    10381 ccagatgatc gtcgaccttt ccggcggcaa ccagcagaag gtgattcttg gcaaatggct
    10441 ggcggcaaat accg
//
LOCUS       DATALY010000134         4140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_375108,
            whole genome shotgun sequence.
ACCESSION   DATALY010000134 DATALY010000000
VERSION     DATALY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375108"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHR42_03995"
     CDS             <1..223
                     /locus_tag="VHR42_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX3026373.1"
                     /translation="EECTGIGAAIIAGVGSGVFPDYRVAREFLKVRETLEPRPQYREM
                     YAHKQKLFEDAHDALLELNHRISRSLQQG"
     gene            complement(393..1793)
                     /gene="gltA"
                     /locus_tag="VHR42_04000"
     CDS             complement(393..1793)
                     /gene="gltA"
                     /locus_tag="VHR42_04000"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX3026374.1"
                     /translation="MPNMSFNKAPMPEQDPRERSRNFSEVSMGYTEALAKSEAQRCLQ
                     CKNRPCMQGCPVNVRIPEFIKLAAEGDYEEAYGVIKGTNSLPAICGRVCPQETQCEQY
                     CVRGKKGEPVGIGRLERFVADWHMANGKNTVDTVKKSGKKVAVIGSGPSGLTCAGDLA
                     KKGYEVTVFEAFHAPGGVLMYGIPEFRLPKDLVLQEIETVKQLGVKFEMNMVIGRIRS
                     LDELTEEGYEAIFVGSGAGLPSFMGIPGENLNGVYSANEFLTRVNLMKAYKFPESDTP
                     VKVGKKVAVIGGGNVAMDSARCAKRLGADQVYIIYRRSEVELPARREEVHHAKEEGIE
                     FKLLTNPSQILGTEKGWVNGIECVRMELGEPDASGRRRPVEVKGSEHLIDVDIVIVAI
                     GTSPNPLIKMTTPGLKTQKWGGIIVDEETGATSREGVYAGGDAVTGAATVILAMGAGK
                     KAAAAIDEFIKNKHRA"
     gene            complement(1786..2631)
                     /locus_tag="VHR42_04005"
     CDS             complement(1786..2631)
                     /locus_tag="VHR42_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX3026375.1"
                     /translation="MFQIIRKAVLTPNIKLMEVDAPYIAQKVEPGQFIILRIREDGER
                     IPLTVADFDREKGTVTIIFQEIGKTTQLLGTLAEGDFILDFVGPLGTPSNFEGVKKAA
                     VIGGGLGTAIAYPQAKKLRGLGAEVDTIIGFRSKDIIIFEKEIAKVSNRLFVTTDDGS
                     NGHKGFVSDVLKNLIEEGNKYDLVVAIGPLIMMKVVSNLTRDYGIKTVVSMNPVMVDG
                     TGMCGGCRVTVGGKTKFACVDGPDFDGHEVDFDEAMRRQSMYQDEEKLSLEKHVCRLG
                     GALNA"
     gene            complement(2702..2920)
                     /locus_tag="VHR42_04010"
     CDS             complement(2702..2920)
                     /locus_tag="VHR42_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026376.1"
                     /translation="MITKDTQVEEIMQINGVISYFIQNGVCPISCSGAFPQSLGNLLK
                     VKKVTDPDAFIEGLNAFVSGPGQETDAR"
     gene            complement(3041..>4140)
                     /locus_tag="VHR42_04015"
     CDS             complement(3041..>4140)
                     /locus_tag="VHR42_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3026377.1"
                     /translation="GEAVMIDKDTLSIILREPLGVVGQIVPWNFPLLMAAWKLAPALA
                     AGNCIVFKPSSTTSLSVLELIKLMSQVLPAGVVNVITGRGSTSGNYMLEHEGFRKLAF
                     TGSTEVGYSVADAAAKKIIPSTLELGGKSANIYFPDAPWEKAIEGLQMGILFNQGQVC
                     CAGSRVFVHESIYDKFLADAVKAFEKVKVGLPWEKDTQMGSQVNANQLKKILSYVEIG
                     KAEGARVATGGYRITENGLDKGSFMKPTILADVDNKMRVAQEEIFGPVVCVIKFKDEE
                     EVIRMANDSEYGLGGAVWTKDINRAFRVARGVETGRMWVNNYNNLPAHAPFGGYKKSG
                     IGRETHKVILDHYTQMKNIYISLSENKFGLY"
BASE COUNT          947 a         1184 c          888 g         1121 t
ORIGIN      
        1 ggaggaatgc accggtatcg gcgccgcgat tatcgccggc gtcgggtccg gagtttttcc
       61 ggactaccgc gttgcccgcg aatttttaaa ggtcagggaa acgctggaac cccgcccgca
      121 ataccgcgaa atgtacgcac ataagcaaaa gctctttgaa gacgcgcatg acgcgctgct
      181 cgagctgaac catcgtatca gccggtcgtt gcaacaagga tgacccgccg cggacaaaca
      241 cgaaagtttt tgacagacga acaacatatt tacagcggaa aatccgcgcg acttttatcg
      301 tcgcgcggat ttctgctgtg ctgttcatat tccccggaac aatattgtga tgcatcactc
      361 cctgccgttc gaaaaagatc tctcgggctt cattaagccc tgtgtttatt tttgatgaat
      421 tcatctattg cggcggcagc ctttttaccg gctcccatgg ccaaaatcac cgtcgccgct
      481 cccgttaccg cgtctccgcc ggcatatact ccttccctgc tcgtcgctcc cgtctcttca
      541 tcaacaatga ttccgcccca cttctgggtt ttaagccccg gcgtcgtcat cttaatcaag
      601 ggattcgggc ttgtaccaat ggcaacaata acgatatcga cgtctatcaa atgctccgag
      661 cctttgactt caaccggcct tcttcttcct gacgcatccg gttcgccaag ttccattctg
      721 acacactcga tgccattgac ccagcctttt tccgtgccga gaatctgtga tggatttgtc
      781 agcaatttaa attcgattcc ttcctctttc gcatggtgaa cttcctcacg cctcgcggga
      841 agctcaactt cggagcgtct gtaaataata tagacctgat cggcaccaag tcgcttggcg
      901 catctggcgg agtccattgc aacattcccg ccgccgatca ccgcgacttt ttttcctacc
      961 ttgacaggcg tatcgctttc cggaaattta tatgccttca taagattgac ccgggtcagg
     1021 aattcatttg ccgaataaac accgttcaga ttttcaccgg gaattcccat aaaagaaggt
     1081 aatccggcgc cggaaccgac gaagatggct tcatagcctt cctccgtaag ctcatctaat
     1141 gaacggattc tgccgatgac catattcatt tcaaacttaa cgccaagctg tttgaccgtt
     1201 tcgatctctt gaagtacaag gtcctttggc agccggaatt cgggaattcc atacatcagt
     1261 acgccgcccg gagcatgaaa cgcttcaaag acggttacct catagccctt tttggcaaga
     1321 tctcctgcgc aggtcagacc ggaaggccca gacccgatta cagcaacttt tttcccggat
     1381 tttttcaccg tatcgactgt atttttcccg tttgccatat gccagtccgc aacaaacctt
     1441 tccagcctgc caatcccgac cggttcacct tttttgcccc tgacgcaata ttgttcgcat
     1501 tgcgtttcct gcgggcagac ccttccgcat atggcaggca gactgttggt gccttttatg
     1561 acgccatagg cttcttcata gtcgccctcg gctgcaagct taatgaattc cggaattcgt
     1621 acatttaccg gacatccctg catgcagggt ctgttcttgc actgaagaca cctttgcgct
     1681 tcggattttg caagagcttc ggtataaccc atcgacacct cggaaaaatt ccggcttctc
     1741 tcgcgcgggt cctgttcggg catgggcgct ttattgaaag acatgttagg cattcagagc
     1801 acctccaagc ctgcaaacgt gcttttcaag agaaagcttc tcctcatcct gatacataga
     1861 ctgccgtctc attgcctcgt caaaatcaac ctcgtgcccg tcaaaatcag gcccgtcgac
     1921 acaggcaaat ttcgtttttc cgcccacggt aacccgacat ccgccgcaca tcccggttcc
     1981 atctaccata accggattca tactgacgac ggtttttatt ccgtaatctc ttgtaagatt
     2041 actgacaacc ttcatcataa tcagcgggcc gatcgctaca acaagatcat atttatttcc
     2101 ttcttcaatc aggtttttga gaacgtccga cacaaaccct ttgtgaccgt tcgacccgtc
     2161 gtcggtggtt acaaacagtc tgttgctgac cttggcgatt tccttttcaa aaatgatgat
     2221 atccttgctg cggaacccga ttatagtatc cacctcggct cccagaccac gcagcttttt
     2281 ggcctgagga taagcgattg ccgtgccaag cccgcccccg atcacagcgg ctttctttac
     2341 tccctcaaag ttggaaggcg tgcctaacgg cccgacaaaa tccagtatga agtctccttc
     2401 tgcaagagtt cccagaagct gagtggtttt gcctatttcc tggaatatga tggtgactgt
     2461 tcctttttcc cggtcaaaat cggctaccgt cagcggtatt ctctccccat cctcacggat
     2521 tcttaaaatg atgaattgcc ccggttccac tttttgagcg atataaggag cgtcaacttc
     2581 catcaacttg atatttggag ttaaaaccgc cttcctgatg atctggaaca ttcaaaagtt
     2641 cccttctttc ctgtattcaa gctttatcgg aaattcagtt gatttccatc cgaagccgtt
     2701 tctatcgggc gtcagtctct tgaccgggcc cggaaacaaa agcattcagt ccttcgataa
     2761 aagcatccgg atcggtcact ttctttactt taagaagatt tcccagactt tgggggaacg
     2821 cgcccgaaca gcttatggga caaaccccgt tttgaatgaa atagctgatt acgccattga
     2881 tttgcataat ttcttcaacc tgggtatctt ttgttatcat gcgcagtcag accttccgtc
     2941 gttatgtcgc cgcatcgttt caagatcaag cgggatcaaa gacattaatg ccatgatgcg
     3001 gggcaacccg catcatggcc aaaaacgtca accaaacgat ttaataaaga ccgaacttgt
     3061 tttctgaaag gctgatatag atgttcttca tctgggtata atgatcgagg atgaccttgt
     3121 gcgtttcgcg gccgatgccc gatttcttat atccgccgaa aggcgcgtgc gcgggtaggt
     3181 tattgtaatt gttaacccac attctgcctg tttcaactcc tctggcaaca cggaacgcgc
     3241 ggttgatatc cttggtccat accgcgccgc caaggccgta ttcgctgtcg tttgccattc
     3301 tgatcacttc ttcttcatcc ttgaacttga tcacgcaaac caccggccca aatatttctt
     3361 cctgcgcgac tctcatcttg ttatcaacgt cagccaggat tgtcggtttc atgaaactgc
     3421 ctttatccaa accattttca gtgatcctgt aacctccggt cgcaactctg gcgccttccg
     3481 ctttgccgat ttccacatag ctgaggatct tcttaagctg gttcgcattg acctgagaac
     3541 ccatctgagt atctttttcc cacgggagcc ccactttaac cttttcaaaa gccttaacgg
     3601 catcggcaag gaacttatca tagatcgact catgtacaaa tactctcgaa cccgcgcagc
     3661 aaacctgccc ctggttaaac agaataccca tctgcaagcc ttcaattgct ttctcccatg
     3721 gggcatccgg gaaatagata ttggcggatt tcccgcccag ctcaagtgtc gaggggatga
     3781 ttttctttgc ggctgcatcg gcgaccgaat agcctacctc cgttgagcct gtgaatgcaa
     3841 gtttccggaa accttcatgt tcgagcatgt aattgccgga agtcgaccct ctgccggtga
     3901 tgacattcac aacacccgcg ggcagcacct gactcatcag ttttatgagc tcgagcacgc
     3961 tcagggatgt cgtcgatgac ggcttgaata cgatgcagtt gccggccgcc agcgcggggg
     4021 ccagtttcca cgcagccatc agcagcggga aattccacgg cacaatctgc cccacaacac
     4081 cgagaggctc tctcaatatg atgctcagcg tatctttatc gatcataacc gcttcccctt
//
LOCUS       DATALY010000135         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_462913,
            whole genome shotgun sequence.
ACCESSION   DATALY010000135 DATALY010000000
VERSION     DATALY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462913"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..506
                     /locus_tag="VHR42_04020"
     CDS             78..506
                     /locus_tag="VHR42_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026378.1"
                     /translation="MKKKAFPMVLTVICLIAFLMLICSGCRKTSTGSSSGANGVVSGA
                     VSGLESAGGKAGSALSGTVSGAVSGAESIGGKAGSALSSAISGAESAGGQAGSALSSA
                     ISGAESGGNSGNTSSGVGSGTVSAGGQAGGSASSSSASSK"
     gene            677..1564
                     /locus_tag="VHR42_04025"
     CDS             677..1564
                     /locus_tag="VHR42_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3026379.1"
                     /translation="MSTYSRLNKIYRTAMEIPFDKSSKIVLISDCHKGDGSWVDDFIH
                     NQNLYYCALKYYYKNGFTYIDLGDSDELWKNKRFDEISNVYTDIFALLHAFYMEKRFY
                     MIYGNHDIVKKYPAFVKKHLSRYYDSHADRYEPLFEGIQVHEGLILKETGSQTKIMLI
                     HGHQGDLLNDMLWRTCRFLARTVWRRLEFFGYKDPTRAAKNYDQRKKVERKIISWVAE
                     NHQIVVAGHTHRPTCPQEGEEPYFNDGSCVHPNCITCIEIVNSELTLVKWDVKTREDR
                     SVYVDKEPIARPRKINENI"
     gene            complement(1561..2181)
                     /locus_tag="VHR42_04030"
     CDS             complement(1561..2181)
                     /locus_tag="VHR42_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3026380.1"
                     /translation="MAKKRVTSKFRQTRSTNTKQKIIEAAEDFFCQNGFYGSSVQRLA
                     AAANVSIGSFYFYFKDKDELLMEVYKKQSERFVRTIEEALGKTEQYQQDRKDWLREFI
                     QNILGAYGNSGKLRFELKALNYENPQIALQRNLIRERTLALMLDSIERCPMINDLKVR
                     HPRTALLITIDLMDSVYDRLSCENPTGNREDILEEYVDAVYKYLLL"
     gene            2347..3618
                     /locus_tag="VHR42_04035"
     CDS             2347..3618
                     /locus_tag="VHR42_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026381.1"
                     /translation="MDFYDSQAWDILQHLFQVKKINDHVLHFTAEFMSELDFSRIKRS
                     VDLLADAFPLIRCGFRESHFHRPVWANRGHTSEQMVSLIETDDSAAAVQCFLCQETDL
                     KNGPQLKVGVIRSGKSDTLCVLINHMLCDAAGFKEVLYTLSSAYTCLENQEKIHIGSK
                     MGDRSIGQILKPYSVKDRIKIYRTKNQLNPHGNQQFDFEGDLSNPFIEIKKIPKDQFR
                     RLKSYAKERGSSINDMILAAYLRVLYRTFGYAAPLPCAIDLRRFLPNRQAGGICNLMS
                     NICCSVGPDIGNSFDDTLSKVKQQMDFQKNDWGSVRNIALLEKLFTVFPYKIAYNLLK
                     KYFSNPPIALTNIGILDKNKLIFGKCVIKNAYMTGSIKYVPNFQVSVTTFDDEATLCV
                     NLYGTEDDRQKVKTFLDDFAAELQNAVLPPC"
     gene            3777..5228
                     /locus_tag="VHR42_04040"
     CDS             3777..5228
                     /locus_tag="VHR42_04040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3026382.1"
                     /translation="MGGHAVYPGFIDAHMHLYSYAVQLSQVDLRGCRSMKELQEAFRE
                     KAKETPKGRWIIGSGFNQEHFTEPKMPTRKELDAVSTEHLILAERCCGHVVCVNTLAL
                     EQSGAEQKLPDPSVLDRGEDGRANGILRETAIAPVTDCIPDPYPSFESKVDALERAAR
                     KVSASGITSVHTVQVKFGGMFENLSLYQELEQQGRLPVRAYVSFDDYPAFGMRTGFGN
                     EKIKTGYYKIITDGSLGSHNAALREPYSDDPTNRGILYYPQETLTEMCRKAYEMDVQL
                     GIHAIGDRALETVVTALETVYAEQKKPDPRFRIIHATCCSPELLRRMKKLPVILDIQP
                     PFITSDLDWAPERLGPERIQQAYLVKTFLDSGFLLTGSSDSPVDPFDPFYGIWSVVCR
                     RASDGHPTNGWRPEECIGVYDALCMYTKNAAYAAFEEKLKGTLEPGKLADFVVASEDL
                     FQIDPFRIKDVAALSTYLGGEEVYRRGESVRQV"
BASE COUNT         1326 a         1371 c         1335 g         1237 t
ORIGIN      
        1 caaccgtttc tggagaagat agtatgaact caaaacgttc attcataaag aaaaattaga
       61 ttaaaggagg caaagccttg aaaaagaaag cttttccaat ggttctgaca gtaatctgtt
      121 taatagcgtt tcttatgttg atctgcagcg gatgcagaaa aacaagtacc ggcagcagtt
      181 ccggggctaa cggcgttgtt tcgggtgccg tctccggtct ggaatccgcc ggcggcaaag
      241 ccggcagcgc actttccggc accgtttccg gcgctgtttc cggtgcggag tccatcggcg
      301 gcaaagcagg cagtgcactt tccagtgcaa tttccggtgc ggaatctgcc ggcggccaag
      361 ccgggagcgc gctttccagc gctatctccg gagcggagtc cggtggcaac agtgggaaca
      421 ccagttccgg tgtcggttct gggacagtat ctgccggcgg ccaagcgggt ggctccgctt
      481 ccagcagctc agcttcaagt aaataagttt ttttgccccg cgtgccacag tttccggtgc
      541 gcgggacaat tcaatcgaac cctattttat gaaaccgccg aacggtatcg ggttcagcac
      601 agccgaacat tccggctttt tctctgaacc cggttatcat cgcattccgg cagttttatg
      661 ctatcggagc agaattatgt ccacttacag ccgtctgaac aaaatatacc gaaccgcaat
      721 ggagattcct tttgacaagt cctcgaaaat cgtcctgatc agcgactgcc acaagggtga
      781 cggaagctgg gtcgacgatt ttatccacaa ccagaatctg tactactgcg cgctgaaata
      841 ctactacaaa aacggattta cctatatcga tctcggcgac agcgacgagc tttggaagaa
      901 caagcgtttc gacgaaatca gcaacgtcta caccgacatt ttcgcccttc tccatgcatt
      961 ttatatggaa aaacgcttct atatgatcta cggcaaccac gacatcgtca aaaaatatcc
     1021 ggcgtttgtc aaaaagcatc tcagccgcta ttatgacagc catgcggacc gctatgagcc
     1081 tttgtttgaa ggcattcagg tacatgaagg gctgattctg aaagaaaccg gctcgcagac
     1141 gaaaatcatg ctgattcacg ggcatcaggg cgacctgctg aacgacatgc tctggcggac
     1201 ttgccgattt ctcgcgcgaa ccgtctggcg caggctggaa tttttcggct acaaggaccc
     1261 gacgcgcgcg gcaaagaatt acgaccagcg aaaaaaggtg gagcggaaaa tcataagctg
     1321 ggttgcggaa aatcaccaga tcgttgtcgc aggccacacc caccggccga cgtgccctca
     1381 ggaaggcgaa gagccgtatt tcaacgacgg aagctgtgtt cacccgaact gcatcacctg
     1441 cattgaaatt gtgaactccg agctcaccct tgtaaaatgg gacgtaaaaa ccagagaaga
     1501 ccggtcggtc tacgtggaca aggagcccat cgccagaccc cggaaaataa acgaaaacat
     1561 ctaaagcagc aggtatttat acaccgcatc cacgtattct tccaggatat cttcccggtt
     1621 gcccgtcgga ttctcacacg acagccggtc ataaacggaa tccatcagat cgatcgtgat
     1681 caaaagtgcc gtcctcgggt gcctgacctt caggtcgttg atcatcggac agcgctcgat
     1741 ggaatccaac atcagtgcca atgtccgctc acggatcaaa ttccgctgca gggcaatctg
     1801 agggttttca taattaagcg ctttcagctc aaaccgcaat tttccggaat tcccataagc
     1861 gccgagaata ttttgaatga attcacggag ccagtccttt cggtcctgct gatattgctc
     1921 agttttgccg agcgcttctt cgatcgttcg aacgaaccgt tcgctctgct ttttataaac
     1981 ttccatcagg agctcgtctt tgtctttaaa atagaagtaa aagctgccga tggacacgtt
     2041 tgcggcggcg gcaagtcttt gaaccgatga accgtaaaat ccgttttggc aaaagaaatc
     2101 ctcggcggct tctatgattt tctgtttcgt attggtactg cgggtctgcc tgaatttact
     2161 ggtaaccctt tttttcgcca ttcttaacct tctccctttt ccataatttt aggataccgg
     2221 ctctcacttg tcaataattt cgaataactc ttcgaaaaag tattgacaag aaaaagaaat
     2281 cggctatact aaattcgaat gattgttcta attttgtata gtctcttata aaggagaaaa
     2341 gatagaatgg atttttacga ttctcaagct tgggatatcc tgcagcacct gtttcaggtg
     2401 aaaaaaatca acgatcacgt cctccatttt accgcggagt ttatgtctga actcgatttt
     2461 tcacggatta agcggtccgt tgaccttttg gcggacgctt ttcccctaat tcgctgcggg
     2521 ttccgggaat cccattttca ccgacccgtc tgggctaacc gcggccatac ctcggagcaa
     2581 atggtgtctc ttattgaaac ggacgattcc gcagcggccg ttcagtgctt tctctgtcag
     2641 gaaaccgatc tgaaaaacgg gccgcagttg aaagtcggtg tgatccggag cggaaaatcc
     2701 gatacgctct gcgttttaat caatcatatg ctctgtgatg ccgcgggttt taaggaagtc
     2761 ctgtatacgt taagcagcgc ttacacttgc ctcgaaaatc aggaaaaaat acatatcggc
     2821 tcgaagatgg gcgaccggag catcgggcag attctgaaac catattccgt gaaagaccga
     2881 attaaaattt accgcaccaa aaaccagctg aacccccacg gaaaccaaca gttcgatttc
     2941 gaaggtgatc tttcaaatcc gtttattgaa atcaagaaaa ttccaaaaga tcaattccgc
     3001 cgtttaaaaa gctatgcaaa agaacgtggg tccagcatca acgacatgat tctggcagcc
     3061 tatctgcgcg tcctgtaccg cacgttcggg tatgccgccc cccttccctg cgccattgat
     3121 ctaagaaggt ttcttccgaa ccgtcaagcg ggaggcatct gcaatttaat gtccaatatc
     3181 tgctgttcgg tcggcccgga tatcggaaac agttttgacg atacactatc caaagtcaaa
     3241 cagcaaatgg attttcaaaa aaacgattgg ggaagcgtcc gaaatatcgc ccttctggag
     3301 aagcttttca ccgtattccc gtataaaatt gcgtacaatc ttttgaaaaa atatttctcc
     3361 aatcctccga ttgcacttac gaatattggg attctggata aaaacaagct tattttcgga
     3421 aaatgcgtaa tcaagaacgc gtatatgacg ggatccatca aatatgttcc gaactttcag
     3481 gtatcggtaa ccacattcga cgatgaagcg actttgtgcg tcaatctata tgggaccgag
     3541 gatgaccggc aaaaggtaaa gacctttctg gatgactttg cggcagaatt gcagaacgcg
     3601 gttcttccgc cgtgttgaag ctttcgcaga gaagagccgg agcaatcgtc ccatcaaggg
     3661 accggctgct ccggctcttc tctatacctg ccgaaccgat tcaccgcgcc ggtaaacttc
     3721 ttccccgccg agataggtcg aaagcgcggc aacatccttg atgcgaaacg gttgatctgg
     3781 gcggacacgc cgtatatccc ggtttcatcg acgcgcacat gcacctgtat tcctacgctg
     3841 tgcagctttc ccaggtggac ctccggggat gccgcagtat gaaagaattg caggaagctt
     3901 ttcgggaaaa agcaaaggaa actccgaaag gccggtggat catcggctcc gggttcaatc
     3961 aggagcattt tacggaaccg aaaatgccga cccggaagga actggatgcc gtcagcacgg
     4021 agcatctcat tctggcggag cgctgctgcg ggcacgtggt ctgtgtaaac acgttagcgc
     4081 tggagcagag cggcgcggag cagaagctgc cagatccatc tgtgctggac cggggcgaag
     4141 acgggcgcgc caacgggatt ctccgcgaga cggccatcgc gccggtgacc gactgtattc
     4201 cggaccctta cccttccttc gaaagcaagg tggatgctct ggaaagagcc gcgcggaagg
     4261 tttcggcttc cggcattacg tcggttcaca cggttcaggt aaaattcgga gggatgttcg
     4321 agaacctgag cctttatcag gagctggaac aacagggcag actgccggtg cgcgcctatg
     4381 tatcgttcga cgattacccg gcttttggca tgcggacggg tttcggcaat gagaagatca
     4441 aaaccggcta ttacaagatc attacggatg gctcgctcgg ttcccacaac gcggctctgc
     4501 gggagccgta cagcgacgat cccaccaacc gcgggattct gtattatccg caggaaacgc
     4561 tgacggagat gtgccgcaag gcctatgaaa tggatgttca gcttggcatt cacgcaatcg
     4621 gcgaccgcgc gctggaaacc gttgtcaccg cactggaaac cgtttacgcc gaacagaaaa
     4681 agccggaccc ccgcttccgc atcatccacg caacctgctg cagcccggaa ctgctccgac
     4741 ggatgaagaa gcttccggtg atcctcgata ttcagccgcc tttcatcacg agcgacctcg
     4801 attgggcgcc ggagcggctt ggcccggaac gaattcaaca ggcgtatctt gtaaaaacct
     4861 ttctcgacag cggcttcctt ctgacgggaa gctccgattc tcccgtggac ccgttcgatc
     4921 cgttttacgg catctggtcc gtggtttgcc ggcgcgcttc ggacggtcat cccacgaacg
     4981 gctggaggcc ggaggaatgc atcggcgtgt atgatgcgct gtgcatgtac acaaaaaacg
     5041 cggcctatgc ggcctttgag gaaaagctca agggtactct ggaacccgga aaactggccg
     5101 acttcgtcgt tgcgagcgaa gacctttttc aaatcgaccc gtttcgcatc aaggatgttg
     5161 ccgccctttc gacctatctc ggcggggaag aagtttaccg gcgcggtgaa tcggttcggc
     5221 aggtatagag aagagccgga gcagccggtc ccttgatggg acgattgct
//
LOCUS       DATALY010000136         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_719857,
            whole genome shotgun sequence.
ACCESSION   DATALY010000136 DATALY010000000
VERSION     DATALY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719857"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VHR42_04045"
     CDS             <1..538
                     /locus_tag="VHR42_04045"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HEX3026383.1"
                     /translation="RGRSNQQMDRTRADHMGMLATVMNSMALADALEQLDVAVRVQTA
                     ISMQQIAEPYIRNRAVRHLEKGRVVVFGCGTGNPFFSTDTAAALRAAEIDADIILKAT
                     MVDGVYDCDPMKNENAVRFDSISHIEVLNKGLGVMDSTAASLCMDNRIPILVFSIQDP
                     QNIVRAVMGEEIGTIVKS"
     gene            611..1162
                     /gene="frr"
                     /locus_tag="VHR42_04050"
     CDS             611..1162
                     /gene="frr"
                     /locus_tag="VHR42_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX3026384.1"
                     /translation="MSECKEYHEKMKKTVSVLQNEFAAIRAGRANPAVLDKIRVDYYG
                     TPTPINQMAAISVIEARTLSIQPWDISTLKSIEKAIQQADLGINPQNDGKIIRLMFPP
                     LTEERRKEYVKNIHKLTEDGKVAIRSIRRDAIDKFKDMKKKSVLTEDDLKDAEKEIQE
                     LTDKFCKEMDGMCEQKVNEIMSI"
     gene            1313..2062
                     /locus_tag="VHR42_04055"
     CDS             1313..2062
                     /locus_tag="VHR42_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX3026385.1"
                     /translation="MRFSFAKMFRSKHRQQDRVLPKHIAIVMDGNGRWAKKKGLPRQA
                     GHSMGANTFRTIARYCNKIGIQYLTVYAFSTENWRRPAEEVKGIMELFREYLIDALTN
                     FRDENIRTRFIGDVSALDENLRKLIREAEESSANKTGLVLNIAVNYGGRQEIVAAARK
                     LAAQAVDGSINADEIDESAVAGVMYTAGQPDPDLIIRPSGELRLSNFLLWQAAYAELW
                     YSDILWPDFRPEHLELAIEDFNRRSRRFGGI"
     gene            2062..2919
                     /locus_tag="VHR42_04060"
     CDS             2062..2919
                     /locus_tag="VHR42_04060"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3026386.1"
                     /translation="MKQRLISAAVGLSIGVVILFFYDTIVLNFALAVIVLLALNEVFA
                     ATHYRDNRGLSVICFAFCLMVPFFHISQLKIAVQIFCFAFILALFILLLYRHKTMKLE
                     QIGTAFLLTLLISFSFSTIVFIRDIYLAPSVKLIGLFYILLVFIGAWMTDAGAYFIGR
                     FFGRNKLAPNISPKKTVEGALGGIAVTVLAFFALSLIFQWVFSGLHCILLVNYVGLAV
                     MSVLSAFFAILGDLSASMIKREYRIKDFGNIMPGHGGVLDRFDSIMFVAPFILMFIQL
                     FPVVQIRII"
     gene            2969..>3739
                     /gene="dxr"
                     /locus_tag="VHR42_04065"
     CDS             2969..>3739
                     /gene="dxr"
                     /locus_tag="VHR42_04065"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX3026387.1"
                     /translation="MNILKNQSISILGSTGSIGTQALDVVEKLGLRVVALSANRNEKL
                     LEQQIRKFRPKIAVLGDQISAGRLRENLRDMEIEILSGEEGVCEAASDSRADLVLNAI
                     VGIAGLRPTIAVLEAKKTLALANKESLVTGGRIVIETARRNGVKILPVDSEHSAIFQC
                     LQGSCDAGREVKKILLTASGGPFYGFNRSRLEKVTPAQALKHPNWSMGAKITIDSATL
                     MNKGLELMEAAWLFGVQPGRIEILVHRESIVHSAVEFVD"
BASE COUNT          931 a          833 c          990 g          985 t
ORIGIN      
        1 gcgtggcagg agcaaccagc agatggaccg gacgagggcc gaccacatgg ggatgcttgc
       61 taccgttatg aactccatgg ctctggccga cgcgcttgaa cagttggatg ttgcggttcg
      121 cgtgcaaacc gctatctcca tgcagcagat tgccgagcct tatatccgca accgtgcggt
      181 tcgtcatctt gaaaagggca gagtagtggt atttggatgt ggaacgggca atccgttttt
      241 ttccaccgat accgccgcgg cgcttcgggc tgcggaaatc gacgccgata ttatcctgaa
      301 agcgacaatg gtcgacggcg tgtatgactg tgacccgatg aaaaatgaga atgcggttcg
      361 gtttgacagc atttcacaca ttgaggtgct taacaaaggt ttgggcgtta tggattccac
      421 cgctgcgtcg ctttgtatgg acaacagaat tcccatactg gttttcagca tacaggatcc
      481 tcagaatatt gtccgcgcgg ttatgggtga agagatcgga acgatcgtca aaagctgacc
      541 gatgaaaaaa gctgccaatc catgcaattt taacttttga atcgccgaca tcggcgaaat
      601 ggaggttttt atgtctgagt gtaaagagta tcatgaaaag atgaaaaaga cggtttccgt
      661 actgcaaaat gagtttgcgg ccatccgggc gggcagggcg aatcctgctg ttctggataa
      721 aatccgcgtc gactactacg gaactcccac accgatcaac caaatggctg ctatttcggt
      781 tatagaagcg aggacccttt ccattcagcc ttgggatatt tcgacgctca agagcattga
      841 aaaggcgatc cagcaagccg atttgggtat aaatccacag aatgatggaa aaataatcag
      901 gctgatgttt cctccgctga ccgaggaacg ccgcaaagaa tatgtaaaaa atattcataa
      961 actgacggag gacggaaaag tcgctatccg atcaatcaga agagacgcca ttgataaatt
     1021 taaagatatg aagaaaaaat ccgttttgac cgaagacgat ctgaaagacg ctgaaaaaga
     1081 aattcaggag ctgaccgaca aattctgcaa agaaatggac ggaatgtgcg aacaaaaagt
     1141 aaatgaaatc atgagcattt agtttgcacg tcctaatagg attcagccaa taacgatgga
     1201 attaaatgcc cctcgtgcga ggggcatttg cataggacaa ttcatgcaga aaaacttatc
     1261 tgtctaaagg gattccctca ttcccgagtt tttttgcttt tcggaggtta caatgcgttt
     1321 ttcatttgca aagatgtttc gttcaaaaca tcggcagcag gacagagttt tgccaaagca
     1381 tatcgccatt gttatggatg gcaacggacg ctgggcgaaa aagaaggggc tgccgcgtca
     1441 ggccgggcac tccatgggcg ccaacacatt ccgaaccatc gcccggtact gcaataaaat
     1501 cgggatccaa tatctgacgg tctatgcttt ttcaaccgaa aattggagaa ggcctgcaga
     1561 agaggtcaag gggattatgg aactgttccg ggagtatctg attgacgcgc ttacaaattt
     1621 tcgggatgaa aatatccgta cccgttttat cggggatgtt tcagcattgg acgagaattt
     1681 aagaaagctc attcgggaag cggaagagag ttctgcaaat aagaccggtc tcgttctaaa
     1741 cattgcagtc aactacggag ggcgtcagga aattgtcgca gcagcaagaa agctggcagc
     1801 gcaggcggtc gacggttcga tcaacgctga cgaaattgac gaatccgcag tcgccggggt
     1861 catgtatacc gcaggtcagc cagaccccga tctcattatc cgccccagcg gagaacttcg
     1921 gctgtcaaac tttttgctgt ggcaggctgc ttatgccgaa ttgtggtatt ctgacatcct
     1981 ctggccggat ttcaggccgg agcaccttga actggcaatc gaggatttta acaggcgcag
     2041 ccgccgattt gggggaattt gatgaaacag agactgattt ccgccgccgt aggtctttca
     2101 atcggtgttg tcatcctgtt tttttatgat accattgttt tgaattttgc gctcgctgtt
     2161 attgtattgc tggcgctcaa tgaggttttt gccgcaacac attaccggga taaccggggt
     2221 ttatcggtta tttgttttgc tttttgtctg atggtgcctt tttttcatat cagccagctg
     2281 aaaattgccg ttcagatatt ctgctttgcc tttattcttg ccctgttcat cctactgctt
     2341 taccggcaca aaacgatgaa acttgagcag attggcacgg catttttgct gacattgctc
     2401 atatcctttt ctttttcgac catcgttttt atccgcgata tttatcttgc ccccagcgtt
     2461 aagctgatcg gcctgtttta tatcctgctg gtctttatcg gagcgtggat gaccgatgca
     2521 ggcgcatatt ttatcggccg ctttttcggc cgcaataaac ttgctcccaa tatcagcccc
     2581 aagaaaacgg tggaaggagc gctgggcggc attgccgtca cggttcttgc cttttttgcc
     2641 ctgtcgttga tctttcaatg ggttttttcc ggcttgcact gtatcctgct ggttaattat
     2701 gtcggtttgg cggtcatgtc ggtgctcagt gcattttttg cgattttggg cgatttgtcc
     2761 gcatcaatga tcaaacgtga atatcggatc aaggattttg gaaatattat gcccggtcac
     2821 ggcggcgtgc ttgaccggtt tgacagcatt atgtttgtgg cgccttttat tctgatgttt
     2881 atccagttgt ttccggttgt ccagattcgg atcatttaaa ggaaaaagcc ggcggacaaa
     2941 ggtattctgc tgataattcg ttaaaaaggt gaatatattg aaaaatcagt caatttctat
     3001 tcttggctcg acggggtcga tcggaacaca ggctcttgac gttgttgaaa aactgggcct
     3061 gcgggttgtt gcgctttccg ccaaccggaa tgaaaaactt cttgaacagc aaatccggaa
     3121 attccggccc aaaatagccg ttctgggaga tcagatttcg gccggacgtc tgcgtgagaa
     3181 tctcagggat atggaaatcg aaattctgtc cggtgaggaa ggggtttgtg aggcggcctc
     3241 cgatagtcgg gcggatctgg ttctaaacgc cattgtcggc atcgccggcc tgagaccgac
     3301 catcgctgtc ctggaagcca aaaagacgct tgccctggcc aacaaggaat ccctcgtgac
     3361 cggaggccgg attgtgatcg agacggccag gcgcaacggt gtgaagattc ttccggtcga
     3421 cagtgagcat tcggcaatct ttcagtgttt gcagggaagc tgcgatgccg ggcgcgaagt
     3481 caaaaagata ttgctgactg cgtcgggagg cccgttttat gggtttaatc gtagccggct
     3541 cgaaaaggta acgcctgctc aggcgctcaa acatccgaat tggtcgatgg gtgcgaaaat
     3601 cacaatagac agcgcaacgc tgatgaataa aggtctggag ctgatggaag cggcttggct
     3661 tttcggggtt cagcccggcc ggattgagat tcttgtgcac cgggagagca tcgttcattc
     3721 cgccgttgaa tttgtcgac
//
LOCUS       DATALY010000137         4967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_204786,
            whole genome shotgun sequence.
ACCESSION   DATALY010000137 DATALY010000000
VERSION     DATALY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204786"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHR42_04070"
     CDS             complement(<1..295)
                     /locus_tag="VHR42_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3026388.1"
                     /translation="MEINERLFGLLAEIKDGQRRLAERLGITDQTISAWKSRKTDPPS
                     KYISTIAEFLNVPVEYLLTGRENVKMETGTGKTANLVSLLNKLNESGILLVTGY"
     gene            628..852
                     /locus_tag="VHR42_04075"
     CDS             628..852
                     /locus_tag="VHR42_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026389.1"
                     /translation="MGKYPIEISEERNAKLKYPFLPTEQYDKVIHNPGGSTILIRYSD
                     KRLTKEDVQRMIEINEKIAYRVAMEKQAAN"
     gene            913..1212
                     /locus_tag="VHR42_04080"
     CDS             913..1212
                     /locus_tag="VHR42_04080"
                     /inference="COORDINATES: protein motif:HMM:NF020349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HEX3026390.1"
                     /translation="MNFEIVSYLDQKNISHSVLGFAYLASAINLAIKNPELKYHTTEL
                     YRAIASTHKTTPTSVERCIRHAIEKSGCAKIVNREFISIAVDSISPNFDWNEDKQ"
     gene            1403..3310
                     /locus_tag="VHR42_04085"
     CDS             1403..3310
                     /locus_tag="VHR42_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026391.1"
                     /translation="MKLNSLSVSNFKAIKSFDFTANGHNVNIFGDNGKGKTSVFDAFS
                     WLLFGKDSAGRKEFDFKFHDETGAPVTGKKLEPLVEAGLEQDGRNLMLKKVYAEQWVK
                     PQGKAVKEYRGSVTEYYVDGLKVKAGEYQAAVDALIQENIFKLITNPHYLPGLKPDKQ
                     REFLVMLAGELNVPVPKELAGLMGYLPFEKFYALQKQNLKEKSKERDTLPAVISEAQR
                     NIPDIQGDPKKIWIDAGKLKVRQAEIENDIRILQSSGSEETRRRSIAETENLILEARN
                     RYTKILSGKNGKVQAKIDSLVAEQSELEISKRRLTSDLSDISERIKRYESNVKDMRDQ
                     YKEAAARKWDGNESCPTCGRPLPPEEIEKAREQFNINKSNQLQQINAEGKSLKSALED
                     LLSEHEKKSGSLPKIESDIATLSERIQKGKSMLTLPDFEQTKEYKALSDKLQTLHSDS
                     SEEPNNDNVRKIDSLREEVQSLQTRIEQMTEQKIRLEQKSDQEKRVAELIEQESKANE
                     ELETIEKNIFLCEEYRKAKSHALESAVNGKFKLVRFKLFETLKTTGEEVETCEAVYTP
                     NGTGYCDVSRSQTTYVGIDICNAFMEHFGVTAPLFVDDAEGVLHELDTDAQVIRLIAS
                     LDDKNLRAEVL"
     gene            3312..3596
                     /locus_tag="VHR42_04090"
     CDS             3312..3596
                     /locus_tag="VHR42_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026392.1"
                     /translation="MVTKTFICDKCKKSVGETELYSVEVELNRPTGGYSSRMHSAHKD
                     ICKECLKTLGIIVDAPEDAKEREKQTSKNQKTFENRICNFLSDLGVLFEE"
     gene            3607..4104
                     /locus_tag="VHR42_04095"
     CDS             3607..4104
                     /locus_tag="VHR42_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026393.1"
                     /translation="MKTNVIKLRFLRHGEPAGREYTYFTNTDVAVGDTVDLESKDGIA
                     QGVVTQIDVPESEIAPFKDRAKTILGKASTPVKEFNVSKAISAQADYCNRTDAPDFAP
                     LNGRCWKCHRNIYEQVGWKIENGRRIQVPLDSPELQHITGIDVEKAGKTLVTGCPHCN
                     RSYCD"
     gene            4116..>4967
                     /locus_tag="VHR42_04100"
     CDS             4116..>4967
                     /locus_tag="VHR42_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEX3026394.1"
                     /translation="MSDKKELQKSEPKTRLTASEAFTQKVMAEFQGKVGGKLELPETQ
                     RNLIQGYFIGVDKAIKAAEENRLRNNKWKRDKEDLPVTWNNVNMTDLALDVMHYSKLG
                     LDITQKNNINAIPFKNNRTNKYDIGFIPGYVGIQLTAYKYAREVPVSVTTELVYKNDT
                     FRIIKKSAEIPYDRYIFEIDNPFDRGDIVGGFGYIEFKEPKKNRLVFMSMHDIEKRKP
                     KYAAAEFWGGDKDKWEKGKKVGTEHIEGWYEEMCLKTLKRFVYGESNIERDPENIDVI
                     FIGSVIFE"
BASE COUNT         1565 a         1117 c         1138 g         1147 t
ORIGIN      
        1 catatccggt gacaaggagt attccgctct cattcagctt attgagaaga ctcactaaat
       61 tagccgtctt tcctgttccg gtctccatct ttacgttttc tctgccagtt aaaagatatt
      121 caacagggac gtttaaaaac tctgcaattg ttgaaatata ttttgacggc ggatctgttt
      181 ttcgtgattt ccatgcacta attgtttgat ctgttattcc taagcgttcg gccagtctac
      241 gctggccgtc ttttatttca gccagaagcc cgaacaatct ttcgttaatt tccacataaa
      301 acacatccta ttattgtaaa taatattatg caattgttgt ttacaactat gcgtttgcag
      361 attatactat atatacagac ggcaacacaa accatcacaa ggcaaacaaa aaccaagcct
      421 atccatagaa taaacttaaa aaatggtttt atgtccttgt gtaatcgcaa aatttagctg
      481 tgggtcttta ctctcaacaa gcaaagatta ccactcgcaa aaccaaatgt caacatttgc
      541 cgtcaatttt cgcaaagaaa ggatgaagtt gaataggaca tgcaaaccgg tacaggcatg
      601 ctacataaga ttaaacgagg tggttagatg ggaaagtatc caatcgaaat atccgaagag
      661 cgaaacgcta aactgaaata cccgtttctt ccgaccgagc aatacgataa ggtaattcac
      721 aatcccggag gttccacaat tttaatccgt tactcggaca aacgcctaac caaagaggac
      781 gttcagcgga tgatcgaaat taacgagaaa atcgcctatc gtgtagcaat ggaaaaacag
      841 gctgcgaatt aaagcgccgg gaatcggcgc gtcaaagaat cggacaagcc aaaaaagaaa
      901 ggagcgttta ctttgaattt tgaaattgta agctaccttg accagaaaaa tatttcccat
      961 tcggtattag ggtttgctta tctcgcatcc gcgatcaacc ttgcaattaa aaatccggag
     1021 ctcaaatatc acacaaccga gctttaccgc gccattgcct ccacacataa aaccacaccc
     1081 acttctgtcg aacgctgcat cagacatgcg attgaaaaat ccgggtgtgc aaaaattgtg
     1141 aaccgcgaat ttatttccat cgccgtggat tccattagcc caaattttga ctggaatgag
     1201 gacaagcaat gacacatcaa atcggtgcca tcatctacgc tgtttttgca tttctcgctc
     1261 tgctgtatgt tggttaccgg gcagatcatc ccaagcaaag gaaaaagccg cctacagccc
     1321 cactacaagc tgtaaacggc aagcaaaata acttcacgac aagagtatca cggaaacggt
     1381 ctcttgtcaa tcggagggct tcatgaaact gaatagtttg tcggtctcta attttaaagc
     1441 aatcaaatcc tttgatttta ccgcaaacgg acacaacgtc aatatctttg gcgataacgg
     1501 aaaaggaaag accagtgtgt ttgacgcctt ttcatggctg ttgttcggaa aagacagcgc
     1561 cgggcgaaaa gaattcgatt ttaaattcca cgacgaaacc ggcgcgccgg tgaccggaaa
     1621 gaaactggag ccgcttgttg aggcggggct tgagcaggac ggaagaaatc tcatgctcaa
     1681 aaaggtttac gctgagcaat gggttaagcc gcagggcaag gctgtcaaag agtaccgggg
     1741 cagcgtcacc gagtattatg tggacggttt gaaggttaaa gccggagaat atcaggccgc
     1801 ggtcgacgcg ctgatacagg aaaatatctt taagctgatt accaaccccc attaccttcc
     1861 cggcctgaaa ccggacaagc agcgtgaatt tctggtcatg ctcgccgggg agctgaatgt
     1921 tccggtcccg aaagaactcg ccgggctgat gggttatctc ccattcgaaa aattttatgc
     1981 gcttcaaaaa caaaatctga aagaaaagtc caaagaacgc gataccctcc ccgccgttat
     2041 ctccgaagcg caaagaaaca ttccggatat tcagggcgat cccaagaaaa tatggattga
     2101 cgccgggaaa ctcaaagttc gtcaggccga gattgagaat gatattcgga ttctccagtc
     2161 ctccggttcc gaggaaaccc gccgccggtc gattgctgaa acagaaaacc tgattctgga
     2221 ggcccggaac cgttacacaa aaattctgtc cgggaagaac ggaaaggttc aggcaaaaat
     2281 tgattctctc gttgcggagc agtccgagct tgaaatatcc aaacgccgcc tgacttccga
     2341 tttgtccgac atatccgaaa gaatcaaacg ctatgagtcc aatgtaaagg atatgcgcga
     2401 ccaatacaag gaggccgccg cccggaaatg ggacggaaac gaaagttgcc cgacctgcgg
     2461 ccgccccctt cccccggaag aaattgaaaa ggcccgggag caattcaata tcaataaatc
     2521 taaccagctt caacagatca acgccgaagg aaaatcctta aaatcagcgc ttgaagacct
     2581 gctgtcagag catgaaaaaa aatccggatc gcttccaaag attgaatccg acattgcgac
     2641 cctttccgaa agaatccaaa agggtaaatc catgctgacc ctgcctgatt ttgagcagac
     2701 caaggaatat aaagccctgt cagacaagct tcaaaccttg catagcgatt caagcgagga
     2761 acccaataac gataatgttc ggaagattga ttccctgcgt gaggaagtcc agtcgcttca
     2821 aacccggatt gaacaaatga ccgaacaaaa aatacgtctg gagcagaaat ccgatcaaga
     2881 aaaacgcgtt gccgagctaa tcgagcagga aagcaaggca aacgaggaac ttgaaaccat
     2941 tgagaaaaat atcttcctct gtgaggaata ccgaaaggct aaatctcacg cgctggaatc
     3001 ggcggtcaac ggaaagttta agcttgttcg gttcaagctg ttcgaaacgc tgaaaaccac
     3061 cggggaagaa gtggaaacct gcgaagccgt ctataccccc aacggaaccg ggtactgtga
     3121 cgtcagccgt tcgcagacaa cctatgtcgg aattgatatc tgtaatgcgt tcatggagca
     3181 tttcggggtt acggcccccc tgttcgtcga cgatgccgag ggcgtccttc atgaacttga
     3241 taccgacgcg caggtgatcc gcctgattgc gtcgctcgat gataaaaatt taagagcgga
     3301 ggttttataa catggtaaca aaaacattta tttgtgacaa atgcaaaaag agtgttggtg
     3361 aaacggaact gtattctgtt gaggttgagc ttaatcgccc tactggagga tattcctcaa
     3421 gaatgcattc agcccataag gacatttgca aagaatgtct gaaaactctc ggaatcatcg
     3481 ttgatgcacc ggaagatgca aaggaaagag aaaaacagac atcaaaaaac cagaaaacgt
     3541 ttgagaacag aatttgcaat tttctttctg acctcggcgt cttatttgaa gaataggaga
     3601 tataacatga aaacaaatgt cattaagctg cgtttcctcc ggcatggaga acctgccggg
     3661 cgcgaatata catacttcac caatactgac gttgcggtcg gtgacacggt cgatctggaa
     3721 agcaaagacg gcattgcaca gggcgtcgtt acgcagatcg atgttccaga atccgagatt
     3781 gccccattta aagaccgggc aaaaacaatt ctcggaaaag caagcacacc tgttaaggaa
     3841 tttaatgttt ccaaggccat ttccgcacaa gcggattatt gcaatagaac cgacgccccg
     3901 gattttgccc ctttaaatgg ccgctgttgg aaatgccaca gaaacattta tgagcaggta
     3961 ggatggaaaa tcgagaatgg gcgaagaata caagttcctc tggattctcc agaactgcag
     4021 catattaccg gaattgatgt agaaaaagct ggtaagacgc ttgtaacagg ctgtccacat
     4081 tgcaaccgta gttattgtga ttaaggagga ttatcatgtc agataaaaaa gagcttcaaa
     4141 agagtgagcc gaaaacccgc ttaacggcaa gcgaggcgtt tacacaaaag gtcatggcgg
     4201 agtttcaggg caaggttggg ggaaagcttg agcttcccga aacacagcga aatttgattc
     4261 agggttattt catcggagtg gataaggcca taaaagccgc cgaggaaaat cggctgcgga
     4321 ataataagtg gaagcgtgac aaggaagacc tccccgtcac ctggaacaat gtcaacatga
     4381 ccgaccttgc ccttgacgtc atgcattatt ccaagctggg cctcgacatc acgcaaaaga
     4441 acaatatcaa tgcgatcccg ttcaaaaata acagaaccaa taaatatgac atcggattta
     4501 ttccgggtta cgttgggatt cagcttacag cctataaata cgcgcgggag gttcccgtct
     4561 cggtcacaac ggagcttgtc tataaaaacg acacattccg aattataaag aaatcagctg
     4621 aaatccccta tgaccgttac atatttgaga ttgataaccc gtttgaccgc ggagacattg
     4681 tcggcgggtt cggttacatc gaatttaagg agccaaagaa aaaccgtctt gtttttatgt
     4741 ccatgcatga tattgaaaag cgtaaaccca aatacgcggc agccgaattt tggggaggcg
     4801 ataaggacaa atgggaaaaa gggaagaaag tcggaacaga gcatatcgag ggatggtacg
     4861 aagaaatgtg cctgaaaacc ctcaaacgat ttgtctatgg ggaatcaaat atcgaacgcg
     4921 acccggagaa catcgatgtc atatttattg gttctgttat ttttgaa
//
LOCUS       DATALY010000138         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_375847,
            whole genome shotgun sequence.
ACCESSION   DATALY010000138 DATALY010000000
VERSION     DATALY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375847"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VHR42_04105"
     CDS             <1..683
                     /locus_tag="VHR42_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX3026395.1"
                     /translation="FNPGLPEVRDLIEKGVLEIVKKYQVDGVVFDDYFYPAQDFNDGA
                     AYKKYGGGKSLDDWRRSNVDTLIRELHEKIGQTRKGVVFGISPFAVWANASSNKAGSD
                     TAASVQSYYTQYADTRMWVKNHLIDYICPQIYWSIGFEKAAYNKVMDWWAQTVQGTGV
                     KLYVAHTVSKVGTDESGWKEPNQIIKQLEYSQKYGDYGGSSFYNYSKLVKNALGVTDT
                     LKAYLSKH"
     gene            1000..>2116
                     /locus_tag="VHR42_04110"
     CDS             1000..>2116
                     /locus_tag="VHR42_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX3026396.1"
                     /translation="MSVVSVALIAAAVALGVDVLKNGPILLKTEEFSWVGGVVTLIDA
                     VTLIAILYFGIRMREWKVIVPSVLQAAAVVYLEVIKRPREPELLVNLDNLSLVMVLIA
                     SVIGPLVAIFALGYMERHEAHHKGKSRQHIFFSVIFLFLTAMNVISISDNFMHLYAFW
                     EITTLCSFLLIGYDKNRKSFLSAKRALWLNSVGGLFFVAGIIFIVNMSGSLSISDLVS
                     GGAVSGYLAAGVLLICCAGFVKSAQLPFQPWLLGAMVAPTPVSALLHSSTMVKAGVYI
                     IVRLSPVFGGHMTGYLVAVVGAFTFMAASALAIGQSNGKRVLAYSTIANLGLIIACAG
                     LGGAEALSAAIFLMIYHAVSKGLMFLCVGTVELGIGSR"
BASE COUNT          443 a          505 c          572 g          596 t
ORIGIN      
        1 atttcaatcc gggtctgccg gaggtgcgcg atttgattga aaaaggcgta ttggagattg
       61 tgaagaagta ccaggtcgac ggcgttgtct ttgacgacta tttttatccg gcgcaggatt
      121 ttaacgacgg cgccgcttac aaaaaatacg gcgggggaaa gtctcttgac gactggagac
      181 gaagcaacgt cgacacgctg attcgggaac tgcatgagaa aatcggccag accagaaaag
      241 gcgttgtttt tggcatcagc ccgtttgccg tctgggcgaa cgcatcttcc aacaaggcgg
      301 gatcggacac cgccgcttcg gttcagtctt attacaccca atacgccgac acgcgcatgt
      361 gggttaaaaa ccatcttatt gattatatct gtccccagat ctactggtcg atcggctttg
      421 aaaaggcggc ctataacaag gtgatggact ggtgggcgca aaccgttcag ggaaccggcg
      481 tgaagctgta tgtggcccat acggtcagca aggttgggac tgacgagtcc ggatggaagg
      541 aaccgaacca gattatcaag cagctcgaat attcccaaaa atacggcgac tacggcggca
      601 gctcttttta caattattca aagcttgtca aaaacgcgct cggggttaca gatacgctga
      661 aggcatactt gagcaaacat taaacgggcg ggaatccccg taaaaattca tagcaaaaaa
      721 gtccgcgtga tgcggacttt tttgcggatt tcatgaaaat ttaaacttga cttaaagctc
      781 tcggtgagct atattataca tggcttttct ttggccatta ttataaatat ggttcttttt
      841 aacgaaaaga tgacgaaagt cgttttttgt gaacagtttt aagattaggg gattgaatta
      901 tgcaaggcgc ttttctcgtt acgctgacac ttttaattcc ggctgtttcc gcgcttgcgg
      961 cgcttttttt tcaaaaagcc ggagtccaga aaaatctttg tgtccgttgt ctctgtcgca
     1021 ttgatcgcgg ctgctgtcgc gcttggtgtc gatgtgctga aaaacggccc cattcttctg
     1081 aaaaccgaag agttttcgtg ggtgggcggc gttgttacgc tgattgacgc ggtgacgctg
     1141 attgcaattc tttattttgg tatccgaatg cgcgaatgga aggtcatcgt gccttctgtg
     1201 cttcaggcgg ccgcggttgt gtatctggaa gtgatcaaac ggccccggga gcccgagctg
     1261 ctggtaaatc tcgacaatct ctcgctggtg atggttctga tcgcgtctgt catcggcccg
     1321 ctcgtggcca tttttgcgct cggctacatg gagcggcatg aggctcacca caagggtaaa
     1381 tccagacagc atattttttt ctctgtgatt ttcctctttt taactgcgat gaacgtgatc
     1441 tcgatttcgg ataatttcat gcatctgtat gccttttggg agattacgac gctttgctcg
     1501 ttccttctga ttggttacga caaaaaccgc aaatccttcc tcagcgccaa acgggcattg
     1561 tggctcaatt cggtcggcgg tttgtttttt gtcgcgggta ttatttttat cgttaatatg
     1621 tccggatctc tttccatttc cgaccttgtt tccggcggcg ccgtatcggg ctaccttgcc
     1681 gcgggcgtgc ttctcatctg ctgtgcgggc tttgtcaaat cggcgcagct gccttttcag
     1741 ccgtggcttc ttggcgctat ggtcgcgccg actcccgttt cggccctgct tcattcctcc
     1801 acgatggtca aagccggcgt ttatatcatt gtccggctgt cgcctgtatt cggaggccat
     1861 atgaccggct atcttgtcgc tgtggtcgga gcgtttacct ttatggcggc ttccgctctt
     1921 gcaatcggcc agtcgaacgg caagagggtg ctggcttact cgacaatcgc gaacctggga
     1981 ctgattatcg cctgcgccgg gcttggcggc gccgaggccc tgtcggccgc gattttcctg
     2041 atgatttacc atgccgtttc caagggcctg atgttcctgt gtgtcggaac ggtagagctt
     2101 gggattggaa gccggg
//
LOCUS       DATALY010000139         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_118172,
            whole genome shotgun sequence.
ACCESSION   DATALY010000139 DATALY010000000
VERSION     DATALY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118172"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /gene="rplJ"
                     /locus_tag="VHR42_04115"
     CDS             complement(<1..120)
                     /gene="rplJ"
                     /locus_tag="VHR42_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX3026397.1"
                     /translation="MPSKKILEEKKQQVAELSEKLKAAHTGVVVSYKGITVADD"
     misc_feature    complement(147..284)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(330..1025)
                     /gene="rplA"
                     /locus_tag="VHR42_04120"
     CDS             complement(330..1025)
                     /gene="rplA"
                     /locus_tag="VHR42_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX3026398.1"
                     /translation="MKHGKKYVDGAKLIDRTKFYDSDEALDLCVKTGTKKFDESVEVH
                     VKLGVDSRHADQQVRGAIVLPNGTGKTVRVLAICKGDAAKDAEAAGADFIGAEEMIQK
                     IQNDNWMDFDVLITTPDMMGLVGRLGKILGPRGLMPNPKAGTVTREIGKAIKEAKAGK
                     IEYRLDKSNIIHCVIGKVSFGKEKLAENFNTLLGAIVKAKPAATKGQYIRSCVVASTM
                     GPGVRINPNKFGA"
     gene            complement(1137..1562)
                     /gene="rplK"
                     /locus_tag="VHR42_04125"
     CDS             complement(1137..1562)
                     /gene="rplK"
                     /locus_tag="VHR42_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX3026399.1"
                     /translation="MAQKVTGFIKLQIPAGKATPAPPVGPALGQHGVNIMAFTKEFNE
                     RTKGEAGMIIPVVITVYADRSFTFITKTPPAAVLIKKACGIDKASGEPNKTKVAKITR
                     EQVKKIAETKMPDLNAASIETAMSMVAGTARSMGIEVVD"
     gene            complement(1678..2199)
                     /gene="nusG"
                     /locus_tag="VHR42_04130"
     CDS             complement(1678..2199)
                     /gene="nusG"
                     /locus_tag="VHR42_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX3026400.1"
                     /translation="MPEEARWYVVHTYSGYENKVASNLAKTVENRQLHDLIQQVLVPT
                     ETVTEVKDEKEREVERKIFPGYVLVKMVLTDESWYIVRNIRGCTGFVGPSSKPIPLTA
                     EEVARLGVEKKEIEVSYGVGDSVKIIGGPLEGFVGIVGEVDKEKNRVRVTVSMFGRET
                     PVELELNQAEAIE"
BASE COUNT          484 a          634 c          552 g          583 t
ORIGIN      
        1 gtcgtcggcc accgtgatgc ctttgtagct gacaacaacg cccgtgtgtg ccgctttcag
       61 cttctcagaa agttcagcta cctgctgttt tttctcttcg agtatctttt tgcttggcaa
      121 attttttcac ctccgctgtc agaatcagcg caatagaaaa gccttttccg ctgccgcaga
      181 aaaggcttca acatacgaac gcatgaagcg ccttattctc ggcaggctgg aaaaccatta
      241 cgcaatcaag cacctgctgt ctttgaataa agaacagttc aatttacttg tagatttaga
      301 ttatatcaca gacccggccg actgtcaaat caggcgccga atttgttggg gttgatgcgc
      361 acgccggggc ccatggtgga agcgacgacg caggaacgga tatactggcc tttcgttgcc
      421 gccggcttcg ccttaacaat agctccgaga agagtgttga agttttcagc cagcttctct
      481 ttgccgaacg aaaccttgcc aatgacgcag tggatgatgt tggacttgtc gagacgatat
      541 tcaatcttac cggctttcgc ctctttgatt gctttgccga tctcgcgcgt gaccgtgccg
      601 gccttcgggt tcggcatcag accgcggggg ccgagaattt taccaagacg tcccacaaga
      661 cccatcatgt cgggcgtagt aatcaggaca tcgaaatcca tccagttgtc attctggatt
      721 ttctggatca tttcctctgc accgataaaa tcagctccgg ccgcttctgc gtcctttgct
      781 gcatcgccct tgcagatggc gagcacacgg acggttttgc ccgtgccgtt cggaagaacg
      841 attgcgccgc ggacctgctg gtctgcgtgg cggctgtcga cgccgagctt tacatggact
      901 tcaacgctct cgtcgaattt ttttgttccg gttttgacgc agagatcaag cgcctcgtcg
      961 gaatcataga atttagtacg atcgatcagc tttgcgccgt cgacgtattt tttaccgtgt
     1021 ttcataggtt ttcctccctg tcaagtggta aaatcggatt tcaagttcct cccaccggga
     1081 atcaggaacc gggaaacgga cggcgcccga aaacgggtcg gcatccgcag ccccaatcag
     1141 tcgacgactt caatgcccat gctgcgggca gtaccggcga ccatgctcat agccgtctca
     1201 atggaagcgg cgttcaggtc gggcatcttg gtctccgcaa tctttttgac ctgctcacgg
     1261 gtaatcttcg cgaccttcgt cttgttcggc tcaccggaag ccttatcgat cccgcatgcc
     1321 ttcttgatca gaacggcggc gggcggagtc ttcgtgatga acgtgaaaga acggtccgca
     1381 taaacggtga tgacaaccgg gatgatcatt ccggcttcgc ccttcgtgcg ttcattgaat
     1441 tcttttgtaa acgccataat attgacgcca tgctggccga gcgcagggcc aaccggcggc
     1501 gccggggttg cttttccggc aggtatctga agcttgataa agcctgttac cttctgtgcc
     1561 atttttactg cacctccaaa attcgtggtt gatggcggag cgaagaagtt ttccgctcct
     1621 cccacagggg aaatcccctc ataacagacg ggtcgccccg ccgattattt caattgctta
     1681 ctcgatggct tccgcctggt tcagttcgag ttcaaccggc gtttcgcgcc caaacatgga
     1741 aacagttacg cgcacgcggt ttttctcttt gtccacttcg ccgacaatac cgacaaagcc
     1801 ttccagcggg ccgccgataa tctttacgga atccccgact ccgtaggaaa cctcaatctc
     1861 tttcttctca acgccgagac gcgcaacttc ttccgccgtc agcggaatcg gtttggaaga
     1921 cgggccgaca aagccggtgc atccccggat attgcggacg atataccagg actcgtcggt
     1981 aagaaccatt ttcaccagca catagccggg gaaaatttta cgttctactt cgcgttcctt
     2041 ttcgtccttt acctccgtga cggtttcggt tggaacaagc acctgctgga tcagatcgtg
     2101 aagctgccgg ttctccaccg tcttcgcaag attggaggca accttgtttt catacccgga
     2161 ataggtatgc acaacatacc atcgtgcttc ttcaggcaaa gaaaacccct ccgtctaaat
     2221 aattattttg caacattcat aatcagccca agc
//
LOCUS       DATALY010000140         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_376108,
            whole genome shotgun sequence.
ACCESSION   DATALY010000140 DATALY010000000
VERSION     DATALY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376108"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..164
                     /locus_tag="VHR42_04135"
     CDS             9..164
                     /locus_tag="VHR42_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX3026401.1"
                     /translation="MHIFIHSLNSRETKDGSAIIYATITINGLEHLKSIISKLSNIEG
                     ILSIRRS"
     gene            258..905
                     /locus_tag="VHR42_04140"
     CDS             258..905
                     /locus_tag="VHR42_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3026402.1"
                     /translation="MKITRIAGGPLPTNCYLLTDDQTGATAVIDPGFESAELTKAVTD
                     AGIENIKAILLTHGHFDHIMGVSRIKKLTNAKIYIHSEDMMFTADNSLNLSGVIIPEK
                     VDAFTADVPLNDGDKIELGNLTIRVIHTPGHTIGGCCYITEDAIFTGDTLMKCSCGRT
                     DFPTGSYPQMLDSLKRLKNLEGDYHIYPGHESETTLDYERKHNPYMGSAAYDIAY"
     gene            889..1464
                     /locus_tag="VHR42_04145"
     CDS             889..1464
                     /locus_tag="VHR42_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026403.1"
                     /translation="MTLLIDGHPFHYEMENLCRVFLPYEKITVTSEACEDTILAYTGL
                     MEKEEGIFLTVSLKSGSFQKENSELVRKEEAADERELERRMAVLLFRIFTEYTGFTPK
                     WGILTGVRPIKLLRGIIDDMGQEKGLDYFQSGLLVSKQKTELSLVTMLREEKLLSLSR
                     PDSFSLYIAIPFCPTRCSYCSFVSSSVEKKP"
     gene            1461..2372
                     /gene="hemZ"
                     /locus_tag="VHR42_04150"
     CDS             1461..2372
                     /gene="hemZ"
                     /locus_tag="VHR42_04150"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HEX3026404.1"
                     /translation="MRLIPKYLALLCKEITHTAAVAAGLGLRLESVYVGGGTPTTLSA
                     EQLKTLLDTVRSCFDLSTCRELTVEAGRPDTITAEKLEALKSRGVTRISINPQTLNDD
                     VLQNIGRRHTTQQTLSAYALAREHGFRNINMDLIVGLPGDSVESFQSTLDRVVSLSPE
                     SVTVHALALKRSARIFQEGGTAYSRDSEAAGQMLDYADSELLKNGYNPYYLYRQSRMI
                     GNLENTGWAKPGFESPYNVFIMDETHSILACGAGAASIVKDPHSNRLERIFNFKYPYE
                     YISRFEEMIIRKDQVKDLYERFRRIPE"
     gene            2344..>3066
                     /locus_tag="VHR42_04155"
     CDS             2344..>3066
                     /locus_tag="VHR42_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HEX3026405.1"
                     /translation="MKDFVEYPNELRSINAQAVSKPEAFVEETEEAYDRNIYDIAKDI
                     AENSNGCKLVMLFGPSSSGKTTTSHLLENTFQKIGIGSAIISLDDFYRGEHQAPLLPN
                     GQHDYECVDALNVPEIKSCLCSLIEANHCDMPVFNFEIHMPYPHKRHVALKEHDIAIV
                     EGIHALNPVVINQLPADKVKKIYISVKQGVHDGEEELFDANDMRLVRRIVRDYNFRGT
                     KPERTLNMWPSVMEGERKYIKPY"
BASE COUNT          854 a          719 c          760 g          733 t
ORIGIN      
        1 ttttcaatat gcacattttt atccattcgc tgaattccag ggaaacaaag gacggcagcg
       61 caattattta tgctacgata accatcaacg gccttgaaca tctgaaaagc attatatcga
      121 agctgtccaa tattgaagga atcctgtcca tccggcgttc ttaatgggcg cctgtggcac
      181 tcaaattgat ttagaacaat gacacaaatt tgtggtccga agatattata ataaataacg
      241 gaggaactgt atttagaatg aaaattacga ggattgccgg tgggcctctg cctacgaact
      301 gttatctgct gacggatgat caaaccggtg caactgccgt tattgatccc ggatttgaaa
      361 gcgctgaact gacaaaagcc gtaacagatg ccggaattga aaatataaaa gcaattttgc
      421 tgactcacgg gcattttgac catatcatgg gagtaagtag aattaaaaag ctgacgaacg
      481 ctaaaattta tattcattcc gaagatatga tgtttacagc ggataattcc ctgaaccttt
      541 ccggcgtcat tatcccggaa aaggtggacg catttactgc ggatgtgccc ctgaacgacg
      601 gtgataaaat cgagctggga aaccttacga ttcgtgtcat tcacacgccg ggccatacca
      661 tcggcgggtg ctgctacatc acggaagacg ccatttttac cggagacacc ctgatgaaat
      721 gcagctgcgg gaggaccgat tttcctaccg ggagttaccc ccagatgctt gactcgctca
      781 aacggctgaa aaatctggag ggcgattatc acatttatcc cggccacgaa agtgaaacga
      841 ctctggatta cgaaagaaaa cataatccgt atatgggaag cgctgcttat gacattgctt
      901 attgacgggc accctttcca ttatgaaatg gaaaatctat gccgtgtttt tctgccctat
      961 gaaaaaataa cggttacttc agaagcctgt gaagatacca tccttgcgta caccggatta
     1021 atggaaaaag aggagggcat ctttcttacg gtcagcctga aatccggctc tttccagaag
     1081 gaaaacagcg agctggttcg aaaggaagaa gccgcggatg aacgggagct ggaaaggcgc
     1141 atggcggtcc ttctgttccg cattttcacg gaatataccg gttttactcc caagtggggt
     1201 attcttaccg gcgtaaggcc gattaaattg ctgcgcggta ttatcgacga catggggcag
     1261 gagaaagggc tggactattt tcagtccgga ttgctggtct caaagcagaa aacagaactc
     1321 agccttgtaa ccatgctccg tgaggaaaaa ctgctttccc tgtcaagacc ggattccttc
     1381 agtctttata tcgccattcc attttgtccc acacgctgtt cctactgctc ttttgtatcc
     1441 tcttccgtgg aaaaaaaacc atgaggctga ttccaaagta cctcgcgctt ttatgcaaag
     1501 aaatcacgca tacagcagcg gttgctgcag gcttgggatt gcgtctggaa tccgtctatg
     1561 tcggcggtgg aacgcccacg acgctttccg cggagcagct taaaaccctt ctcgatacgg
     1621 tgcggagctg ctttgacctt tccacctgcc gcgaattaac ggtagaggcc ggcagaccgg
     1681 ataccatcac cgcggaaaag ctggaagcgc tcaaatcaag gggcgtaaca cgcatcagta
     1741 tcaacccgca gacgctgaac gacgacgtgc ttcagaatat cggacggcgc cacacgacac
     1801 agcagacgct ctccgcgtat gcgttggcac gggaacacgg tttcaggaat atcaacatgg
     1861 atttgatcgt cggccttccc ggcgacagcg tagagagctt tcaatccacg cttgaccggg
     1921 tcgtttcact ttcgccggaa agcgttacgg ttcatgcgct tgccctcaag cggtcggcaa
     1981 ggatttttca ggagggagga acggcttaca gccgcgattc cgaagctgcg ggccaaatgc
     2041 tggattatgc ggattccgag ctgctgaaaa acggctacaa tccgtactac ctttaccggc
     2101 agagccgaat gattgggaat cttgaaaata ccggatgggc aaaaccgggt tttgaaagcc
     2161 cctacaatgt ttttatcatg gatgaaacac acagcattct tgcctgcggg gcaggtgccg
     2221 ccagtatcgt caaagacccg cattccaacc ggctggaacg gattttcaac tttaagtatc
     2281 cctatgaata tatcagccgc tttgaggaaa tgattattag aaaagatcag gtgaaagatt
     2341 tatatgaaag atttcgtaga atacccgaat gaattgcgca gcataaacgc tcaggctgtt
     2401 tcgaagccgg aagcttttgt ggaggaaacg gaagaagctt acgacaggaa catttacgat
     2461 atcgcaaagg atattgcgga gaacagcaac ggctgcaaac tggtgatgct tttcggaccc
     2521 tccagcagcg ggaaaactac cacctcgcat cttttggaaa acacctttca gaaaatcgga
     2581 atcggctccg ctattatttc actggatgat ttctaccgtg gggagcatca ggcgccgctt
     2641 cttccgaacg gtcagcacga ctacgaatgc gttgacgcgc tgaatgtacc ggaaataaaa
     2701 agctgtttgt gcagcctgat cgaagcgaat cactgcgata tgcccgtatt caattttgaa
     2761 atccatatgc cgtacccgca taaacgccat gtggccttaa aggagcacga cattgccatt
     2821 gtagaaggta ttcacgcatt gaatccggtc gtaataaatc agctgcctgc cgataaagta
     2881 aaaaaaatat atatcagcgt aaagcagggg gttcacgacg gggaagagga gctctttgac
     2941 gcaaatgata tgcgtctggt acgcagaatc gtgcgagact ataatttccg cgggacaaaa
     3001 ccggagcgca cgcttaatat gtggccttcg gttatggagg gtgagcggaa atacattaag
     3061 ccttac
//
LOCUS       DATALY010000141         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_720764,
            whole genome shotgun sequence.
ACCESSION   DATALY010000141 DATALY010000000
VERSION     DATALY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720764"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1564
                     /locus_tag="VHR42_04160"
     CDS             191..1564
                     /locus_tag="VHR42_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEX3026406.1"
                     /translation="MKIKQKSARPIIKLQGRPSLRQSQFLRARTKYVAYGGARGGGKS
                     WALRRKCLILAFSFPGVRILLLRRTLQELRENHTIPLKRELGESIRYIAEEKAFVFGN
                     GSRIKLGYCDDQSDVDQYQGQEYDVICMDEGTQFTEFQFICLSACLRGGNPGCPKHFY
                     ITCNPGGVGHSWVKRLFIDRCYRKGENPEDYTFIPARVFDNDFLMKYNPDYLRQLESL
                     PEDLKKAWLYGEWEVFAGQYFTEFSRALHVCRPFEIPAGWRRYYVQDYGLDMLAGLWI
                     AVSAQGRAYVYREIYRSGLVVSDAASEIRSLERADEEVFLRLAPPDLWGRTKDSGKSV
                     IEIFADNGLYFSRADNNRVQGWMALREWLKPIGNPDGSNGAKLKIFENCTNLIRTLPL
                     LQFDPKKPNDVADQPHELTHAPDALRYFASSRPLSGGCSEAQGETGRLLPDENDLTQD
                     YLFAGWR"
     gene            1596..1850
                     /locus_tag="VHR42_04165"
     CDS             1596..1850
                     /locus_tag="VHR42_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026407.1"
                     /translation="MMELCCLTGVFTGGCVCVFGMWAFLKGQRSMFQIKNGEAPTELF
                     AAKAIRGGGEKSGGESDQPDIAEQLRSMFGDLPDEGGEED"
     gene            1847..>2374
                     /locus_tag="VHR42_04170"
     CDS             1847..>2374
                     /locus_tag="VHR42_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026408.1"
                     /translation="MTAEEIWSRYQSGVNFKSEIDLYEMVAKNERFFAGDQWAGVNAP
                     DLPKPVVNFIKKACQQKIAEVASCPVRVSFSALEFGDGVVLPEKENIPAATDGDAQLL
                     NGLFESDWDRLGMDSLNLDGLTDACISGDYLLYNYWDDTAETGQAARGRVCVEIVDNV
                     NYYPADPNDKEIQRQR"
BASE COUNT          594 a          551 c          716 g          513 t
ORIGIN      
        1 tggcaacatt tattttaaaa tccgctttcg ggtaccgtga caagcaggat gatgcgagaa
       61 acagcgcccc gatccgagtg gaggtcaccg acgaattaaa aaaatacgcc gtataaaatg
      121 ttcgtaggca aagaaaacag acggtttctt ctgtaataat caaatgaaac caaaaatatg
      181 gcggttagaa atgaaaatca aacaaaaaag cgcaagacca atcatcaaat tgcagggcag
      241 accctcttta agacaatcgc agtttctccg ggccaggaca aaatatgtgg cttacggcgg
      301 agcgcgggga gggggcaaat cctgggcgct gagaagaaag tgcctgattc ttgccttcag
      361 ctttccgggt gtaaggattc ttcttctccg gcgcaccctc caggagctcc gggaaaacca
      421 cacaattccg ctcaaaaggg agcttggtga gagcatccgt tatattgcgg aagagaaagc
      481 gtttgttttt ggaaacggaa gcagaattaa gcttggttac tgtgacgacc agtccgacgt
      541 cgaccagtat caggggcagg agtacgacgt gatctgcatg gatgagggaa cccagtttac
      601 cgaattccag tttatctgcc tgtcggcctg cctgagagga ggaaacccgg gatgcccgaa
      661 gcatttctat attacctgca atccgggcgg tgtggggcat tcgtgggtca aaaggctttt
      721 catcgaccgg tgttaccgga aaggggaaaa tccggaggat tacacattta ttccggcgcg
      781 ggttttcgat aatgattttt taatgaaata taaccccgat tacctgcgcc agctggagag
      841 cttgccggaa gatctcaaaa aagcctggct ttacggtgaa tgggaggttt tcgccgggca
      901 gtattttacc gaattttcca gagccctgca tgtctgccgg ccgttcgaga ttccggcggg
      961 atggcggagg tattatgtgc aggattatgg gctggatatg ctcgccgggc tgtggattgc
     1021 ggtgtcggcg caggggcgcg cctatgttta cagggaaata taccggagcg gcctggtggt
     1081 gagcgacgcc gcctcggaaa tcagatcgct ggaacgtgcg gacgaggagg tctttttacg
     1141 cctggcgccg cccgatttat ggggcaggac caaagacagc ggcaagagcg tgattgaaat
     1201 cttcgccgac aacgggcttt atttttcccg ggcggataac aaccgtgtcc agggttggat
     1261 ggctttgcgg gagtggctca agccgattgg aaaccctgac ggctccaacg gggcaaaact
     1321 gaaaattttt gaaaactgca cgaatctgat ccgtactctg ccgttgctgc agttcgaccc
     1381 taaaaagccg aacgacgtcg ccgatcagcc gcatgagctg acccacgcgc cggacgcgct
     1441 gcgctatttt gcttcctcgc gtcccctttc ggggggatgc tccgaggcac agggagagac
     1501 cggccgctta ttgccggatg agaatgattt gacgcaggat tatctgtttg cgggctggag
     1561 gtaaccggcc cgaacgggat gaggaggaaa gaaaaatgat ggaattatgc tgcctgacag
     1621 gggtttttac gggcggatgc gtctgtgttt ttggaatgtg ggcgttttta aagggccaga
     1681 ggagcatgtt tcaaattaaa aacggcgaag cgcccacgga actattcgcg gcgaaggcta
     1741 tacggggagg cggtgaaaaa tccggcggtg aatccgatca gccggatatt gcggagcagc
     1801 tcaggtcaat gttcggcgac ctgccggacg aagggggcga agaggattga cggcggagga
     1861 aatctggagc cggtaccaat ccggtgtcaa cttcaaaagc gaaattgatc tctatgagat
     1921 ggtggcgaag aatgaacgct tttttgcagg cgaccagtgg gcgggggtca atgcgcccga
     1981 tcttccaaag ccggtcgtta attttattaa aaaagcctgc cagcaaaaaa tcgccgaagt
     2041 agcctcctgc ccggtcaggg tgtcgttcag cgctttggag tttggcgacg gcgtcgtttt
     2101 gccggaaaaa gaaaacatcc ccgccgctac cgacggagac gcacaactgc taaacggcct
     2161 gtttgaatcc gactgggaca ggctcgggat ggattccctc aatcttgacg ggctgaccga
     2221 cgcatgcatc agcggggact atcttcttta taactactgg gacgatacgg ctgaaacagg
     2281 gcaggcggcc cggggacggg tctgcgtcga gattgtagac aatgtcaact actacccggc
     2341 cgacccgaat gataaagaga ttcagagaca gcgg
//
LOCUS       DATALY010000142         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_376330,
            whole genome shotgun sequence.
ACCESSION   DATALY010000142 DATALY010000000
VERSION     DATALY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376330"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VHR42_04175"
     CDS             <1..580
                     /locus_tag="VHR42_04175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026409.1"
                     /translation="VGQKDQFSRTGLYVGGTSLFYGTDILLGAFERLNETKGLNIRLI
                     LVCREGEGDELLLPYAGRPWLEVIHASGDEALEPIYRRSDFGIFSGRRDPYMDFSMPV
                     KLFEYLSRALPIVTTDCVEAANFVEKNGVGIVAQDNPQSFAEAVAGLYDDPDCLPRLR
                     KNAERALRGGNLWVHRAERAAQEIMQSRNLRS"
     gene            602..1717
                     /locus_tag="VHR42_04180"
     CDS             602..1717
                     /locus_tag="VHR42_04180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026410.1"
                     /translation="MRIAVLSAANSVHTVRWVSALAERGHQVALYSLPDHPASIGAVP
                     GNAEVHYLSRGGVAGYWLGSGELRRRLREFRPDLLNAHYATGYGTLARRCGFRPLLLS
                     VWGSDVYDFPRQGVINRKILMKNLADATAIASTSFAMARQVRRIYPAAGEIFVTPFGV
                     DADVFRPVGKPCAGRLTIGIVKTLEPKYGVEDLIRAFALLCERLTREERLPEKGISLE
                     IYGGGSLRGGLENLSRRLAPGLVQFHGPAPHAEVPKILSGFDLFCAPSVSDSESFGVA
                     AVEAMACGIPVVVSDVDGFQEVVVDGVTGLVVPRKNPEALADGMYRLACDSGLRERFG
                     RAGREHVLKNYAWPGCVDKMETALRATVERYGRPLPF"
     gene            1738..2958
                     /locus_tag="VHR42_04185"
     CDS             1738..2958
                     /locus_tag="VHR42_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3026411.1"
                     /translation="MRIMLINHYAGSEYHGMEFRPYYLAREWVKQGHEVVIVAADYSH
                     LRKNNPESAGLTEQSMDGIRYLWVKTPPYHGNGAARAKNMAAFVFRLLHEAGSLAHKY
                     QPEAVIASSTYPFDIYPAQKIARLSSARLYFEIHDLWPLTQIELYGLSRANPYVMLLQ
                     KAEDYAFTHAEKVISVLPQAYLHMEERGVSRAKFAYVPNGVSLEPGGEDGPIPYQDLI
                     GELKSQGKFIVLYLGGFAKANALDEFVKSASLVGENVELLLVGDGVKNQELMDLSKGL
                     GDKNIRFLDPVPKNKVRGLLNLADCLYIGARRCSLYRYGVGMNKLYDYMMASRPVISG
                     VEASNDPVAESRCGLTVRPEDEREIAGAVRKLSSMPRRELEMMGANGRAYVESRHDYR
                     VLAKKFEDLLKGRS"
     gene            2958..4121
                     /locus_tag="VHR42_04190"
     CDS             2958..4121
                     /locus_tag="VHR42_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX3026412.1"
                     /translation="MKKRDTFLPYSQPYWGKEELEGVQDAIRSNWWSKGPKTIELEKI
                     FARYVGAKYAVAVNSCTAALHTSLVARGIGPGDEVITTPMTFCSSANVIVHTGAKPVF
                     ADVDEKTGCISPDCVEAAVTGKTKAVIPVHYAGQPCDMDRIEEIADRNHLFILEDAAH
                     ALYTQYKGKMVGSMNTTAFSFYSTKNISTGEGGMLTTNDEDFAEKARVISCHGMSRNA
                     WNRYGKGGSWRYDVEFAGYKYNMTDLQASLGIVQLGRLEAMQQKREVYSRIYDEELSG
                     LPGIRTPQDGGLGRSSRHLYIIRIQKEELDIGRDEFIEELNAMNIGTSVHFIPVHLHP
                     FYQQNYGTRPGDFPVAEKIFEQIISIPLYPSMSEEDVRYVAGAIRDISQKHAK"
     gene            4075..4791
                     /locus_tag="VHR42_04195"
     CDS             4075..4791
                     /locus_tag="VHR42_04195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019190484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX3026413.1"
                     /translation="MLQEPFGIFRRNMQNESLRPYREHLRRRKAGLALKRAFDLLTAA
                     VCVALLSPFYLIAAAAVKLTSKGPVFYYQERVGKDARKIRVMKFRTMVVGADRRGQLT
                     VGSDDQRITRVGKILRASNFDEFPQMFNVLKGDMSIVGVRPEVSRYVACYTPEDYATL
                     LLRPGMASITAILYRHENEMLSGSADPEKDYIEKILPEKMKFNREYIRNFSFGSDLII
                     LLKTILCVFQKDQMLGKREQ"
BASE COUNT         1106 a         1175 c         1417 g         1123 t
ORIGIN      
        1 ggtcgggcaa aaggatcagt tcagccgcac cggtctttat gtgggaggca cctccctgtt
       61 ttacggaacc gatattctcc ttggcgcttt tgaacgattg aatgaaacaa aggggcttaa
      121 cataaggctt attctcgtct gccgtgaagg cgagggcgat gaactccttc tgccgtatgc
      181 cggcaggccg tggctggaag tcatccatgc ttccggcgat gaggcgctgg agcctattta
      241 ccgtcgaagt gattttggga tattttccgg acgcagggat ccgtatatgg atttcagcat
      301 gccggtcaag ctgtttgagt acctttcccg tgcgctgccg atcgtgacga ccgactgtgt
      361 cgaagcggca aactttgtcg agaaaaacgg tgtggggatt gtggcacaag acaatccaca
      421 gtcctttgcc gaggcggtgg ccggccttta cgacgaccct gactgtcttc cccggcttcg
      481 taaaaacgct gagagggcgc tcaggggagg caatctatgg gtacaccgtg ccgaacgggc
      541 cgctcaggag attatgcagt ctcgaaactt acggagttaa aggaaggcgg gaataaacaa
      601 tatgcgcatt gcggtcttat ccgccgcgaa ttccgttcac accgtccgat gggtttcggc
      661 tctcgccgag aggggccatc aggtggcact ttattcgctg ccggaccatc ctgcctccat
      721 tggtgcggta ccgggcaacg cggaagttca ttatctcagc cgggggggcg tggcgggcta
      781 ctggcttgga tcgggggaac tcaggcggcg gctgcgcgaa ttccgtccgg atctgttaaa
      841 cgcccattat gctaccggtt atggaacact tgcgcgcaga tgcggctttc gtcccctgct
      901 gctgtcggtc tggggcagcg atgtctatga ttttccccgg caaggcgtta taaaccgaaa
      961 aattcttatg aaaaatctcg cggacgctac cgccatcgcc tccacaagct ttgcgatggc
     1021 ccgtcaggtc cgcaggattt acccggcggc cggagaaatt tttgtaacgc cgtttggcgt
     1081 ggatgccgat gttttcaggc cggtcgggaa accttgcgcg gggcggctga ccattggaat
     1141 cgtgaaaaca cttgaaccga aatacggcgt tgaagatctg atccgggctt ttgctctgct
     1201 ttgtgaaagg cttacgcggg aggaacgact cccggaaaag ggaatcagcc ttgaaatcta
     1261 tggcgggggc tcgcttcggg gcgggctgga gaacctgtca aggcggctgg cgccgggtct
     1321 ggttcagttt catggcccgg ctccccacgc tgaggttccg aagatacttt ccggatttga
     1381 tttgttttgc gcgcccagtg tgagtgacag cgagagtttc ggtgtcgcgg cggttgaggc
     1441 gatggcctgc ggtattccgg tcgttgtcag cgatgtcgac ggttttcagg aggtcgtcgt
     1501 ggacggtgta accggtctgg tggttccacg gaaaaatcct gaggcgcttg ccgacgggat
     1561 gtatcggctt gcctgcgact ccgggctgag ggaacggttc ggccgggcgg ggcgcgagca
     1621 tgtgttgaaa aattacgcgt ggcccggttg cgtggataag atggaaacgg cgctcagggc
     1681 gaccgtggag cgttacggaa ggccgctgcc tttttaacgg tggcggcgga ggcagttatg
     1741 agaatcatgc tgattaatca ctatgcgggt tcggaatacc atgggatgga attccgtccg
     1801 tattatctgg cgagggaatg ggtaaaacag ggacatgaag ttgttatcgt cgccgcggac
     1861 tactctcatc tgagaaaaaa taatcccgag tccgcgggtc tcaccgagca gtctatggat
     1921 ggaattcggt atctatgggt caaaacgccc ccttaccatg gcaacggcgc ggcccgtgca
     1981 aaaaatatgg cggcatttgt ttttcgtctt ttgcatgaag ccggtagtct tgcgcataaa
     2041 tatcaaccag aagcggtaat tgcttcttca acttatccgt ttgatattta tcctgcgcaa
     2101 aaaattgccc ggctgtcatc agccaggctc tattttgaaa ttcacgacct ctggccgctg
     2161 acacaaatcg agctatatgg gctttcacgg gcgaatcctt atgtgatgct gctgcaaaaa
     2221 gcggaggatt atgctttcac tcacgcggaa aaggtcattt cggtgctgcc acaggcctac
     2281 cttcatatgg aggagcgggg agtttcgagg gcgaaattcg cctatgtgcc aaacggagtc
     2341 tcgctggagc ccgggggaga agatggaccc atcccgtatc aggacctcat cggtgaactg
     2401 aaaagtcagg gaaaattcat tgtcctgtat cttggcggtt ttgcaaaagc caacgcgctg
     2461 gatgaatttg tgaaatcggc atctctcgtt ggagaaaatg tggaactttt gctggtcggg
     2521 gacggagtaa aaaatcagga actcatggat ttgagcaagg ggttggggga caagaacatc
     2581 cgctttctcg atcctgtgcc gaaaaacaag gtcagagggc ttctgaatct tgccgactgt
     2641 ctctatatcg gcgccaggcg ctgcagcctt taccgctacg gcgtgggaat gaacaagctt
     2701 tatgactata tgatggcctc acggccggtc atctccgggg ttgaggcttc caacgacccg
     2761 gtcgcggagt cccggtgcgg gctgacggtc agacccgagg atgagaggga gattgccggc
     2821 gctgttcgga aattgtcatc catgccccgc cgcgagcttg aaatgatggg agcgaacggg
     2881 cgtgcgtatg tggaaagccg tcacgattat cgtgtgctgg ctaaaaaatt tgaggatttg
     2941 ctaaaaggaa gatcgtaatg aaaaaaaggg atacattcct gccttattcc cagccctatt
     3001 ggggcaagga ggaactggaa ggcgttcagg acgccattcg ttcaaactgg tggtcgaaag
     3061 ggcccaaaac cattgaatta gaaaagatat ttgcgcgtta tgtaggggcg aaatatgccg
     3121 tcgcggtcaa ttcctgcacc gccgcgcttc atacatcgct tgtggcgcgt gggatcgggc
     3181 cgggagacga agtcatcact accccgatga ctttttgttc ttctgccaat gtgattgtcc
     3241 ataccggcgc gaagccggtg tttgccgatg tggatgaaaa gacgggctgc atcagtcccg
     3301 actgtgtgga agccgctgtt accggaaaga ccaaagcagt gattccggtt cattatgcgg
     3361 gccagccctg tgacatggac cggattgagg aaatcgcgga tcgcaatcac ctgtttattc
     3421 ttgaagacgc ggcgcatgcc ctctacacac aatataaggg aaaaatggtc ggctcgatga
     3481 atacgacagc cttcagtttc tattcgacca aaaacatcag cacgggcgag ggcgggatgc
     3541 tgaccacaaa cgacgaagac tttgcggaaa aggcaagggt gatttcctgc cacggcatga
     3601 gccggaacgc atggaatcgc tatggcaaag gcggaagctg gaggtatgac gtcgagtttg
     3661 ccggatataa atataatatg accgatcttc aggcgtccct cggcatcgtt cagctcggcc
     3721 ggcttgaagc catgcagcag aagcgcgaag tttacagccg gatttatgac gaggaacttt
     3781 cgggcctccc cggtatcagg actccgcagg acggggggct cggccgcagc tcccgccatc
     3841 tctatatcat ccggattcaa aaagaagaac ttgatatcgg cagggatgaa tttattgagg
     3901 aactgaacgc gatgaatatc ggaacaagcg tccattttat tcccgtccat ctccatccgt
     3961 tttatcagca gaattacggc acaaggccgg gcgatttccc ggtcgccgaa aaaatctttg
     4021 aacagatcat ctcaattccc ctgtacccat caatgagtga agaggatgta agatatgttg
     4081 caggagccat tcgggatatt tcgcagaaac atgcaaaatg agtcgctccg gccttaccgg
     4141 gagcatctcc ggcgccgaaa agcgggcctt gcgctcaaac gcgcgtttga ccttctgacc
     4201 gcggcggtct gtgtcgccct gttgtcgcct ttttatctga tcgcggcggc agccgtcaag
     4261 ctgacgtcca aaggccccgt cttttattat caggagcgcg ttggaaaaga cgccaggaaa
     4321 atacgcgtga tgaagttccg taccatggtg gtcggcgccg accggcgcgg gcagcttacg
     4381 gtggggagcg acgaccaaag gattacccgg gtggggaaga ttctgcgcgc atccaatttt
     4441 gacgagtttc cgcaaatgtt caatgtctta aaaggggata tgtcgattgt gggcgttcgc
     4501 ccggaggttt cccgctatgt tgcctgttac acgccggaag attacgctac gctcctgctt
     4561 cggccgggta tggcttccat aaccgcgatt ctttaccggc atgaaaacga aatgctcagc
     4621 ggcagcgccg acccggaaaa agattacata gagaaaatac tgcccgaaaa aatgaaattc
     4681 aatcgtgaat atatccgtaa tttttctttt ggatcggatc tgatcattct tcttaaaacc
     4741 attctttgcg tttttcaaaa agatcagatg ctgggaaaaa gagagcagta atgactgttc
     4801 atctataaaa aggcccccac a
//
LOCUS       DATALY010000143         5216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_549757,
            whole genome shotgun sequence.
ACCESSION   DATALY010000143 DATALY010000000
VERSION     DATALY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549757"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VHR42_04200"
     CDS             complement(<1..245)
                     /locus_tag="VHR42_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX3026414.1"
                     /translation="MIKAIIFDFDGLIVDTETLWYKCFKKVFEDLGHAFPLDLFEQSI
                     GTEFKLNDYIRKYIKIPNSVEVVGDRAKQLFDQEIDNL"
     gene            665..3142
                     /locus_tag="VHR42_04205"
     CDS             665..3142
                     /locus_tag="VHR42_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3026415.1"
                     /translation="MVQNYSNLLIVFSMLYLFLGIYVFDKNRESSVNRAFFLLCISES
                     IWAFGYAFMIHAGNIGEANFWRIIAAAGWCFFYGVWLDFTLQLYGKTRSIWNTLLLYM
                     PGIIIFIYTMMLPKELVLSRLNGGWDDNYPLNLMEKFFILYFSTYILAGIAVIYLWGG
                     KSRKRRENKQAMVIIAADILTIILSVLSEYSNVIFGVKTFSLTVIVLSVAMAGLLFAI
                     KKYKLIDFMPKIVSEYLFKEVNTPIFLIDSDLRIKYANKEASSLTGYELDDLRGASFS
                     RITDVNLLLLFEDKEEIEKCEINLISWEGKTLQFEMSGKFVYDEFREKIGILIILHDV
                     YERKKAERILENYNIELKSAVRKRTAELEKANQVLKDEVAERKAAEAKIRSMGYYDHL
                     TKLPNHRYFDEYITRYIEEIKDQSKMFAVLYLDLDNFKLINDTFGHQHGNGLLKHYTA
                     ELGRIITENCFFARVGGDEFLVLVKNIDPAGYQREISDFANKILDVFDNPYIISNTES
                     FVTVSIGIAVYPMDGTDSQSMIKNADTAMYEAKNNGKNNFRFCSEEIKNKLLCKTRYR
                     NLLYRAQEKQQFSLVYQPQVNIITKRITGVEVLVRWKLPNDALVPPTEFIPIAEETGL
                     IVPIGYWIFKKACLDYKKLTELGFGELTLAVNISTVQLSQSYFADEVLKIISDIGIDP
                     RKIEMEITERVTLEQSGESIENLDRIKKSGIRVSIDDFGTAYSSYMNIKHLPVDKIKI
                     AKEFVNGINTNEKDAAIINSIIHLSHNLKLAVIAEGVETNNQLKFLIQRNCDEVQGFL
                     FYRPMEFDALKSVLKSSSNGEPDTCMI"
     gene            3285..4001
                     /locus_tag="VHR42_04210"
     CDS             3285..4001
                     /locus_tag="VHR42_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026416.1"
                     /translation="MLSIRSITKTYDRDKVKAVDNISLEVENGEIFGFIGPNGAGKTT
                     TIKMITGILPIDSGNIVVNGFDISANPIEAKKSMGYVPDTHDIYDRLTGAQYLNFIAD
                     VYDVPSQERQVQIERYLEMFDMKQAAGELIKSYSHGMKQKIILTGALLHSPRLWILDE
                     PLTGLDPKSAHLLKEEMHSHCARGNTVFFSTHVLEVAEKLCDRIAIIKQGKIIAVGTM
                     EELRSGKDESLERIFLELTE"
     gene            4053..>5216
                     /locus_tag="VHR42_04215"
     CDS             4053..>5216
                     /locus_tag="VHR42_04215"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026417.1"
                     /translation="MKQYFILLKLQIRSQFRLGGLKKGSGSGLGGALKKIGMAVLILF
                     ALAELLGVYTFMMLSLYQGAKALNSPEIVLTVSAACCALVIFAFGIFFILSVLFFAKD
                     SEFLISLPVRQNHIFATKFTLIALREYPITLFIMLPPVIIYGIGSGRGAAYYVLALCA
                     IILLPMIPLALSSFLSLLMMRAVARMRHREVFATIGGILIFVLVFFGQTMLSSRLSSS
                     NSAELVKAMTARGGLVTQASRLYPPSEWLTFALSGNSAPKYFLYLLGLSAAAVYIVIA
                     LSSRIYQKGMQVQFESPKGSSRKKYRFSSSSPVITIVRNEWISIIKTPVYAMNTLLGI
                     ILAPLVLIMPAFSGGSDTSSMIRLLHILFDRFRLIAPCQLGADDVHHQRKRGGG"
BASE COUNT         1412 a         1100 c         1267 g         1437 t
ORIGIN      
        1 agattatcaa tctcctgatc aaacagctgt ttcgccctgt cgccgaccac ttcgactgaa
       61 ttagggatct ttatgtattt tctgatgtaa tcgttgagct taaattcggt tccgatcgac
      121 tgctcgaaca aatccagagg gaaggcgtga cccaagtctt caaagacctt tttaaaacat
      181 ttataccaga gtgtttctgt gtcgacaatt agtccgtcaa aatcgaaaat tatggctttt
      241 atcattttgt accccttatg aattgaaatg ttgtagaagg atcattctga ttttttaaca
      301 tcagtccgca tcgaatagca gtctcttcat ttccagatca actatatcac tatttccaat
      361 agggtgtcaa tcaccatttg tcatctgaag cggcggaaaa aggctcaaat tgcatgataa
      421 ttggacaacg aatttcagtg cccggcctgc acgggcgcaa aggaaaatat tgaacaattc
      481 aaccatttat gataaacttt ttatcgtagg gattttgtaa agtcggattc atctgcgctg
      541 aaacaacaaa atttctgcct gctttttaga ttgatactat gcggctgctg attcatcaga
      601 atttataaca acatctgaag cgaatcagcc gccgctttaa aagccagaga acagaggggt
      661 tgttatggta cagaattatt ccaatttact gatcgtgttt tccatgcttt atctgttcct
      721 tggcatttat gtgtttgata aaaacaggga atcgtctgtg aacagggctt ttttcctgtt
      781 atgtataagc gaaagtatct gggcgtttgg ctatgctttt atgattcatg cggggaatat
      841 tggcgaagca aatttctggc ggatcatcgc ggccgcgggc tggtgctttt tttatggggt
      901 ctggctggat tttacccttc agctctatgg caaaaccaga agcatctgga atacgctgct
      961 gctttatatg cccggaatta ttatctttat ctatacgatg atgttaccga aagaactggt
     1021 gctgtcgagg cttaacgggg gatgggatga caactatccg cttaacctga tggagaagtt
     1081 tttcatactt tacttttcca cttatattct ggcggggatc gccgtcattt atctctgggg
     1141 cggaaaatcc cgcaagcggc gggaaaataa gcaggccatg gtcatcatcg cggctgacat
     1201 tctaacgatt attctgtcgg ttttatcgga atattccaac gtaatattcg gagtgaaaac
     1261 cttttctcta acggtcattg tcctttctgt tgcaatggcc ggtctgctct ttgcgataaa
     1321 aaaatataaa ttgatcgatt ttatgccgaa aatcgtttcg gaatatttat ttaaagaggt
     1381 caacacaccg attttcctta ttgacagcga tttaagaatt aagtatgcca acaaggaagc
     1441 ttcaagtctg accggttatg agctggatga tttacggggt gcatcattca gccgaattac
     1501 ggatgtgaac cttctgctgc tatttgaaga taaggaagaa attgagaaat gcgaaatcaa
     1561 tctgatttct tgggaaggca aaactcttca atttgaaatg tcggggaaat ttgtctatga
     1621 tgagtttcgt gaaaaaattg gaattctgat tattcttcat gatgtttatg aacgcaaaaa
     1681 ggccgagagg attcttgaaa attacaacat agaactcaaa agcgcagtca ggaaacggac
     1741 ggcggagctg gaaaaggcca atcaggtctt aaaggatgag gtagcggaac gcaaggcagc
     1801 ggaagcaaaa atccggagca tgggttatta cgaccatttg accaaactgc cgaatcatcg
     1861 gtattttgac gaatatatca cgcgttatat tgaggaaatc aaagatcaga gcaagatgtt
     1921 tgccgttctt tatcttgatc tggataactt caagctgatt aacgatacgt ttggacatca
     1981 gcatggaaac ggattattaa agcattacac cgcggaatta ggaaggatta ttacagaaaa
     2041 ttgctttttt gccagggtcg ggggcgacga gtttctggtt ctggtcaaaa atatcgaccc
     2101 ggcgggttat cagcgggaaa tttccgattt cgcgaataaa attcttgatg tatttgacaa
     2161 tccatatatt atttcgaata ccgaaagctt tgtgacggtc agtataggca tcgctgttta
     2221 tccgatggac ggaaccgact cgcagtcgat gataaaaaac gccgataccg ctatgtatga
     2281 agcaaaaaac aacggaaaga acaacttccg gttttgttcg gaggaaatta aaaacaagct
     2341 gctgtgcaag actcgttacc gcaatctgct gtatcgggct caggaaaaac aacagttttc
     2401 ccttgtttat cagccccagg tcaatatcat caccaaacgg atcaccgggg ttgaggtgct
     2461 cgtgcgctgg aaactgccga acgacgcgct tgtgccgccg acggaattta tcccgattgc
     2521 cgaggaaaca gggctgatcg ttccaatcgg atactggatt tttaaaaaag cctgtcttga
     2581 ttataaaaag ctgacggagc ttggattcgg cgaattgact ttggcggtca acatttccac
     2641 cgtgcagctg agccagtcgt attttgccga tgaagttttg aagatcatca gtgatatcgg
     2701 gatcgacccc cgaaaaatcg aaatggaaat cacagagagg gtcacgcttg agcagagcgg
     2761 ggaatccatt gaaaatcttg acaggatcaa aaaatccggc atccgggttt ccatcgacga
     2821 tttcggaaca gcctactcct cttacatgaa catcaagcac ctgcccgtcg acaaaattaa
     2881 aattgccaag gaatttgtca acggaatcaa tacgaacgaa aaagatgccg caattatcaa
     2941 ctcaatcatt cacctttccc ataacctgaa actggctgtc attgcggaag gcgtggaaac
     3001 aaacaatcag ctcaaatttt taattcagcg caattgtgat gaggttcagg gattcctgtt
     3061 ttaccgtcct atggagtttg acgcgctgaa gtccgttctg aaatcaagca gtaacggcga
     3121 gccggatacc tgtatgattt aattacagta atttcagtta gaattgcgag atagagtgtg
     3181 tttttctccc ccgccaaagg cgggggagaa aaacaagaca attctacatc ttaattgcaa
     3241 agctgtagaa ttcagggtga ttaaaagaaa aaggggaaaa gatcatgctt tcaatccgat
     3301 ccattaccaa gacctatgac agggataagg tcaaagcggt cgacaatatc agcctcgaag
     3361 ttgaaaatgg ggagattttc ggatttatcg gcccgaacgg agccggaaaa accacaacga
     3421 ttaaaatgat taccggtatc ctgcccattg acagcggaaa tattgtggta aatggctttg
     3481 atatttcagc aaacccgatt gaggcaaaaa agtctatggg gtatgtgccg gacacgcatg
     3541 acatttacga ccggctgacc ggcgcgcagt atctgaactt tattgccgac gtttacgatg
     3601 tcccgtctca ggaaagacag gttcaaattg agcgctatct tgaaatgttt gatatgaagc
     3661 aggccgcggg ggagctgatt aaaagctatt cccacggaat gaagcaaaag attattctga
     3721 ccggcgcgct gctgcattcg ccgcggctgt ggattctcga tgaaccgttg acggggcttg
     3781 accccaagtc ggcccatctg ctcaaagaag aaatgcattc ccactgtgcg agaggcaaca
     3841 ccgttttctt ttccacccat gtgctggaag tcgccgaaaa gctctgcgac cggatcgcca
     3901 ttataaaaca gggcaaaatc attgccgtcg gaacaatgga ggagctccgg agcggaaagg
     3961 acgaatctct tgagaggata ttccttgagc tgaccgagta ggaactttaa aaaaagtttc
     4021 gattcttcaa gagattacgg aggtttgatt cgatgaaaca atatttcatc ctgctgaaat
     4081 tgcagatccg ctcgcagttc cgcctgggcg ggctgaaaaa aggcagcgga agcggcttgg
     4141 gaggcgctct gaaaaaaatt ggaatggcag ttctgattct gtttgcactc gccgaacttc
     4201 tgggcgttta tacctttatg atgctaagcc tgtatcaggg agccaaagcg ctgaattcac
     4261 cggaaatcgt cctgaccgtt tcggcggcct gctgtgcgct tgtgatcttt gcgtttggta
     4321 tcttttttat tctcagtgta ttgtttttcg caaaagacag cgaattttta atttcactgc
     4381 cggtgaggca gaatcatatt tttgccacga aattcacgtt gattgcattg agagagtatc
     4441 cgatcacgct gtttatcatg cttcctcctg taatcatata cgggatcggc tccggccggg
     4501 gcgccgccta ttatgttttg gcgctgtgcg caatcattct gcttccgatg attccgctgg
     4561 ccctgtcgtc gtttttatcg ctgctgatga tgagagcggt tgcgcgcatg agacatcggg
     4621 aggttttcgc caccataggc ggaattctga tatttgtgct ggtgtttttc ggccaaacga
     4681 tgctgagcag ccggctgagc tcttccaaca gtgcggagct ggtgaaagca atgacggcgc
     4741 ggggcggtct ggtaacgcag gcaagccgac tctatccgcc gtccgagtgg ctgacatttg
     4801 ctttgagcgg aaattcggcg ccgaagtatt tcctttattt gctggggctg tcggccgcgg
     4861 cggtttacat tgtcatcgct ttgtcgtcgc ggatctatca gaagggcatg caggtgcagt
     4921 tcgagtcgcc gaaagggagc tcccggaaaa aataccgctt ttcctcgtcc tctccggtaa
     4981 ttaccatcgt gagaaatgaa tggatatcga ttatcaaaac ccccgtttat gcaatgaata
     5041 ccctgctggg catcattctt gctccgctgg tgctgatcat gccggcgttc agcgggggaa
     5101 gcgatacctc gtcgatgatc cgtttgcttc atattttgtt tgatcgcttc cgtctgattg
     5161 ctccatgtca gcttggggcg gatgacgtcc accatcagag aaaacgcggt ggtggt
//
LOCUS       DATALY010000144         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_292893,
            whole genome shotgun sequence.
ACCESSION   DATALY010000144 DATALY010000000
VERSION     DATALY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292893"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VHR42_04220"
     CDS             complement(<1..488)
                     /locus_tag="VHR42_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX3026418.1"
                     /translation="MQTADEKLEKLGNSVCAIVSKEYTFGADAVLLADFSAPAGGRAC
                     EAGTGCGVIPLIWCGKNSALRVDAVEIQAGAAELAARAAAYNGMNDRIRIINDDLRTT
                     RELPSGAYDLVACNPPYQKSGSGQMSGDRPKRLARYEVEGTAEDFISFSSRLLKNGGR
                     LCL"
     gene            complement(619..789)
                     /locus_tag="VHR42_04225"
     CDS             complement(619..789)
                     /locus_tag="VHR42_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3026419.1"
                     /translation="MAYKINDDCISCGACASECPVSCISEGDGKYVVDESVCVDCGTC
                     ASVCPVEAPKQA"
     gene            complement(920..1789)
                     /locus_tag="VHR42_04230"
     CDS             complement(920..1789)
                     /locus_tag="VHR42_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEX3026420.1"
                     /translation="MTEVIGVRFKSVGKVYYFDPCNIKFAIGDRVIVETARGIECGEV
                     VMPNRAVNEDSIVKPLKNVIRAASPEDIARVEENSRKEKEAFRICLKKIADHKLGMKL
                     IDVEYTFDNNKILFYFTADGRVDFRELVKDLASVFRTRIELRQIGVRDEAKMLGGLGV
                     CGKPFCCATFLGDFQPVSIKMAKEQGLSLNPTKISGTCGRLMCCLKYEQTAYEDLLKK
                     TPKTGAYVLTPDGKGMVEDFNLLTGMLKVRLDSPQDSPLKVIHRRDVKVLRDGEIKPD
                     KHSAAASPNTEKN"
BASE COUNT          435 a          529 c          396 g          473 t
ORIGIN      
        1 aggcacagcc ttccgccgtt ctttaacagt cttgacgaaa atgaaataaa atcttccgcc
       61 gtcccctcga cttcataccg cgcaagccgc ttcgggcgat cgccgctcat ttggcccgaa
      121 ccggatttct gataaggcgg gttgcaggcg accaaatcat aagcgccgga gggaagctcc
      181 cgggttgtcc tcaaatcgtc gttaatgatc cggatccggt cgttcatccc gttataagcg
      241 gctgctcgcg cggccagctc agccgctccg gcctgaatct ccacagcatc cactctcagc
      301 gcggagtttt ttccgcacca gatcagcgga ataaccccgc agcctgtccc cgcctcgcag
      361 gctcttcctc ccgcaggggc ggaaaaatcg gcgagcagca ccgcatccgc tccaaaggtg
      421 tattcctttg aaacaatggc acaaaccgaa ttccccagct tttccagctt ctcgtctgca
      481 gtttgcatgc ccgcctcccg ttttcagacc ggcagccgac ctttttatcg gcagcgcact
      541 tcaggcagct ccggtttcta tgtataaaag aaagcctgcg cgaccacgca ggcttccgtt
      601 atgtattact ttgaaagact atgcctgttt gggagcctca accggacata cgctggcgca
      661 agttccgcag tcaacacaaa cgctttcatc taccacgtat ttgccgtcgc cttcagagat
      721 acatgaaacc gggcattctg aagcacaggc tccgcagctg atgcagtcgt cattaatttt
      781 ataagccatt cttagcacct ccttaaaaat ctattcttaa ttttatcacg aatctgtaaa
      841 atttcaatag ttattcaaaa aggcaaatat tgattcatac aaaagcaaca acttatcaaa
      901 tatgcctcaa cgaattttgt cagtttttct ctgtattcgg cgaagccgcc gcgctgtgtt
      961 tgtcgggttt gatttctcca tcgcgcagga ccttcacatc acggcgatga ataaccttta
     1021 agggtgaatc ctgcggactg tcaagacgga ctttcagcat tccggtcaga aggttaaagt
     1081 cctccaccat ccctttgccg tcgggagtca atacataagc gccggtcttc ggtgttttct
     1141 ttaacaaatc ctcataggcg gtctgctcat atttcaggca gcacatcagc ctgccgcagg
     1201 ttcccgaaat tttggtcggg ttgagcgata atccctgctc ttttgccatt ttaatcgaca
     1261 caggctggaa atcacccaga aaagtcgcgc agcagaaagg ctttccgcaa acgccaagcc
     1321 cgccaagcat tttcgcttca tcgcgcacgc cgatctggcg cagctcaatg cgggtgcgga
     1381 aaacagaagc cagatccttg accaactccc ggaaatccac acggccgtcc gcggtaaaat
     1441 aaaagagaat cttattgtta tcgaaagtat attcgacatc aatcagcttc atacccagct
     1501 tatggtctgc aatttttttc aggcagatac ggaaggcttc cttttctttt ctggaatttt
     1561 cttcaaccct cgcgatatct tccggagacg cggcccgaat gacatttttc agcggcttga
     1621 cgatgctgtc ttcgttgacg gcccggttgg gcatgacaac ttcaccgcat tcgattcccc
     1681 gggcggtctc aacaatcacg cggtcgccta tggcgaactt tatattgcac gggtcaaaat
     1741 aataaacctt gcccacgctt ttaaaacgaa ctccgattac ttcggtcatc gagttttact
     1801 tcccttcttc ccgctgcgaa cacttttaaa ttc
//
LOCUS       DATALY010000145         7890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_118989,
            whole genome shotgun sequence.
ACCESSION   DATALY010000145 DATALY010000000
VERSION     DATALY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118989"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VHR42_04235"
     CDS             <1..540
                     /locus_tag="VHR42_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX3026421.1"
                     /translation="RYPIAYLLIALFIVVATVNSVNLTDGIDGLCSSVTFIAALAFML
                     TSKLLGFPVYAALSAALAGGCLGFLVWNFHPAKIFMGDTGSLFLGGILCAIAFGINMP
                     LLLVPFGIIYIGETLSVIIQVISFKSTGKRVFKMSPIHHHFEMSGWSEVKIVGVFSLI
                     TAVFSVAAVVWVARYMGIL"
     gene            569..1699
                     /gene="ftsW"
                     /locus_tag="VHR42_04240"
     CDS             569..1699
                     /gene="ftsW"
                     /locus_tag="VHR42_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX3026422.1"
                     /translation="MAGKKGLKKRLIRGGFDLPFFVLVMILLMLGLVMMFSASYAYAY
                     AHYNNSFYFIKKQAVWAVLGIFIMYVTATFDYHRFHKVAYPILAASYVFLVVVLFMPK
                     SHGVRRWIPKPINFQPSEVAKFAVILVFAHIIAKNYSKMKTFKYGVLPYITILGSMAG
                     LLVLEPHLSGTILILTIGFTLMFVGGTSLKWFGAGGGIAAGAILIIVLFTHLIDYAKS
                     RLTYWLDPFSDAKGLGWQTIQSLYAIGSGGLMGLGLGNSRQKYLYISEPQNDFVFSIV
                     CEELGFIGATIVILLFALLVWRGFVIAMRAPDKFGSILAIGLTTQVGLQAALNIAVVT
                     NSIPNTGISLPFFSYGGSSLVMLLFQMGIVLSVSRFSSVEQT"
     gene            1788..2894
                     /gene="murG"
                     /locus_tag="VHR42_04245"
     CDS             1788..2894
                     /gene="murG"
                     /locus_tag="VHR42_04245"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX3026423.1"
                     /translation="MKILFAGGGTAGHINPAIAVASFIRRRYPDADIRFAGTPSGMES
                     KLVPKAGFRIYPIEIRGFRRKISLYNLGTIRRLATSFSRADRILKDFQPDVVVGTGGY
                     VSGPVLYRAAKRKIPTVIHEQNSFPGVTSKILSRYADQIMIASEDAAKYLKHPERITV
                     TGNPVREEIVGYPREKARRELGMDERPVILSFGGSLGARTMNQAIADLMELDSKSARY
                     QHIHAAGSYGWGWMPALLREKGVNVKAPGIRLSEYIHDMPRCLAAADLVICRCGAITL
                     SELQVLGKPSILIPSPNVAENHQYHNGGSMEKRCAAILIEDDKLSGKLLMEKIDQLFS
                     HPGRLEKMSRASLKMAIPDANERIFKVVRSVLKM"
     gene            3027..4280
                     /gene="murA"
                     /locus_tag="VHR42_04250"
     CDS             3027..4280
                     /gene="murA"
                     /locus_tag="VHR42_04250"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX3026424.1"
                     /translation="MSRLIVEGGNPLVGEQLVQGAKNSALPVLTSTILCDSPSMIHNC
                     PNLSDVDASCRILSCLGCKVTRDDHSVFVDPSGIQGHDIPDDLMREMRSSIVFLGAIC
                     ARMGSAHISFPGGCELGPRPIDLHLTALRKLGIQIKEDHGWLNCRVIGKLHGANIVLS
                     FPSVGATENIMLAACTAEGDTVIVNAAREPEISDLADFLNQCGAKVSGAGESVITIRG
                     VQSLTGREHRVIPDRIVAATLMAAAAITGGEITLRNVNPLHLSPVVPVFAEAGCVINE
                     YSDTMVIKRSGPLRPVKYVRTMPYPGFPTDAQAPVMAMSAIAAGTSMFIENIFENRYK
                     HAAELTRLGAKINVEGKVAVVEGVRRLLGAPVEATDLRGGAALVVAALAAEGTTQISG
                     IKHIDRGYENIEGVLSEIGAAIRRE"
     gene            4370..5158
                     /locus_tag="VHR42_04255"
     CDS             4370..5158
                     /locus_tag="VHR42_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX3026425.1"
                     /translation="MSERKPRKTEPYRGRELAPRKKRRRKPTPFAYVVILITVAACAV
                     SLSAMIFFKVETIRITGKTRYSAQQILDAGGVGKGMNLFAIDKKKASGKICSALPYIR
                     SVEIKLMPLSTVELAVEEDKPSSIVALTSGYAVLDQESKVLEIRPGIKNLNLPMVKGM
                     KVSYAQPGSVLRVAEQSQIQILSRIRQSMTQAKLKKVSTIDITNDLEITASYDQRIKI
                     EIGTASDLSQKMNLAAYVIANKISASEKGSLDVTEASKAYFDPS"
     gene            5188..5937
                     /locus_tag="VHR42_04260"
     CDS             5188..5937
                     /locus_tag="VHR42_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX3026426.1"
                     /translation="MNKIACLVSGLRKYSGQSVLLLICFILGFGLTIQIKSVYQLNSG
                     AASNDIKRAEDLQKRVSELGQKNKDLYNQVEKLQSALDDYRAKAAKSGSYADTLNKQL
                     QDAEILAGLADVHGKGVIVTLDDAEQSASTNAFVVHDSDILQVLNELRDAGAEALSIN
                     DERILSTSEVRCAGNTISVNDNRYSAPFVIKAIGNPDKLRSAIMMKDGIADVLGQWGI
                     KVNVESSDGLYIKAYEGMPKYQYAGAVNNKK"
     gene            5974..6327
                     /locus_tag="VHR42_04265"
     CDS             5974..6327
                     /locus_tag="VHR42_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX3026427.1"
                     /translation="MFSLIGLAVGVLAALLYPGYIPQQYLIYVAVAILAALDSIFGGI
                     NARFRQKFRKSIFISGFFVNTALAALLTFVGQLLQIDLYLAAIVVFGTRIFQNLAEIR
                     RFILNYYAKKDKISL"
     gene            6517..7674
                     /gene="ftsZ"
                     /locus_tag="VHR42_04270"
     CDS             6517..7674
                     /gene="ftsZ"
                     /locus_tag="VHR42_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX3026428.1"
                     /translation="MAFELDKDFDNIVQIKVVGVGGGGNNAVNRMISTGVQGVEFISI
                     NTDKQALYLSQATHKIQIGDKLTRGQGAGANPEKGQRAAEESRDEIADALKGTNMVFI
                     TAGMGGGTGTGAAPVIASIAKELGILTVGIVTKPFLFEGRRRMDQAESGITALREQVD
                     SLVVIPNERLKLVSEQKITLANAFCVADDVLRQGVQSISDLIKVPGLVNLDFADVTAV
                     MKDAGYAHMGVGRSSGKDKAEEAARMAISSPLLETSINGARGVIINITSSADIGLEEV
                     EFASSMITNAADPSANIIWGAAFDETLEDEMNITVIATGFDSGLNLNETHSVFQAAKA
                     SRASASTPENASAPSPKAKEQPQHHGAPAPAQHSQADDEYFDIMSIFNRRK"
BASE COUNT         1933 a         1876 c         2125 g         1956 t
ORIGIN      
        1 cggtatccca tcgcctattt gctgatcgcc ctgttcatcg tggttgctac agttaactcg
       61 gtgaatctga ccgatggaat agacggtctg tgctcctcgg taacttttat cgccgctttg
      121 gcgtttatgc ttacgtccaa actgctggga ttccccgttt atgccgcgtt atctgcagct
      181 ctggccggag ggtgtctcgg attcctggtt tggaattttc accctgcaaa aatatttatg
      241 ggcgacactg gttcgctgtt tctcggcgga attctctgcg ccattgcatt tggaatcaat
      301 atgccgcttc tgcttgtgcc gttcggcatt atctatatcg gtgagactct gtcggtgatt
      361 attcaagtga tttcatttaa atccaccggc aaacgcgtct ttaaaatgag cccgattcat
      421 catcattttg aaatgtcggg atggagcgag gtcaaaattg tcggcgtctt ttcgctgatt
      481 accgctgtct tttcggtggc cgcggttgta tgggttgccc ggtatatggg catcctttaa
      541 tggatgaaat gatctggagg catgaatgat ggcaggaaaa aagggattaa aaaaacgtct
      601 tattcgtggt ggatttgacc tgccgttttt tgtgctggtt atgattttgc tgatgcttgg
      661 cctggtgatg atgttctcgg caagttacgc ttacgcctac gcccattaca acaatagttt
      721 ttattttatt aaaaagcagg ctgtctgggc ggtgctgggt attttcatca tgtatgtcac
      781 tgcgacgttt gactatcacc gtttccataa agtcgcctat ccgattctgg cggcttctta
      841 tgtgtttctg gtagtcgttc tgtttatgcc gaaatctcat ggcgtcaggc ggtggatccc
      901 aaagccgatc aacttccagc cttctgaggt ggccaagttt gccgttattc ttgtgtttgc
      961 gcatatcatc gcaaagaatt acagtaaaat gaagaccttt aaatatgggg ttcttcctta
     1021 tatcaccatt cttgggagca tggcggggct gttggttctc gagcctcacc tttccggcac
     1081 aattttgatt ttgaccattg gctttacgct gatgtttgtc ggcggaactt ctctgaaatg
     1141 gttcggggcc ggcggaggta ttgccgccgg ggctatttta atcattgttt tgttcacgca
     1201 tttgatcgat tacgcaaaat caaggttaac ttactggctt gatcctttca gtgacgcaaa
     1261 aggattgggc tggcagacca ttcagtcgct ctatgctatc ggatcgggtg gactgatggg
     1321 gctcgggctt ggaaactcga gacaaaaata cctttatatt tccgaacccc agaatgactt
     1381 tgttttttcc atcgtttgtg aagaactcgg ctttattggc gcaacaattg tcattttatt
     1441 atttgcactt ctggtctgga gaggattcgt cattgccatg agggcgccgg ataaattcgg
     1501 ctcgattctt gccatcggtc ttacgacgca ggttgggctt caggcggcgc tgaatatcgc
     1561 ggttgtgacc aattccattc caaataccgg aatcagtttg ccattcttca gttacggcgg
     1621 cagttctctc gttatgcttt tgttccagat ggggattgtc ctgtccgttt cacgtttttc
     1681 gagtgtcgaa cagacatagc cgtcgaaagt ggctatgatt ttcctgaaaa aacagacagg
     1741 ccctttgact gataccctta tgatatcaga cgcgggaggt taataaattg aagattcttt
     1801 ttgcgggggg cggcacggcg gggcatatca accctgcaat cgcggtcgct tcctttatca
     1861 ggcgaagata tcctgatgcc gatatccggt tcgcgggtac tccatcggga atggaatcca
     1921 aacttgttcc taaggctggg ttcaggattt atccaattga aatccgcggg tttcgccgaa
     1981 aaatcagtct ttataacttg ggcaccatcc ggcggctggc gacatctttt tcccgcgccg
     2041 accggatttt aaaagatttt cagcctgacg tcgtggtggg gacgggcgga tatgtcagcg
     2101 ggccggtgct ctaccgggcg gcaaaacgaa aaattccgac cgtaatccat gagcaaaatt
     2161 ccttcccggg agtcacgtcg aaaattttgt cccgatacgc cgatcaaatc atgattgcgt
     2221 cagaggacgc ggccaaatac ctgaaacatc cggagcggat taccgtaacg ggaaaccccg
     2281 tgcgggaaga aattgtcggt tatccgaggg aaaaggcccg cagggaactg ggaatggacg
     2341 aaagaccggt gatcctttcc tttgggggaa gcctcggagc aagaaccatg aatcaggcga
     2401 tcgccgatct gatggagctt gattccaaat cagcaagata tcagcatatc cacgcggccg
     2461 gttcctacgg ctgggggtgg atgccggccc tgctcagaga aaagggcgtc aatgtaaaag
     2521 cacccgggat tcgcctgagc gaatacattc acgacatgcc aagatgtctc gcagctgcgg
     2581 atttggtgat ctgccgctgc ggcgccatca ctctcagcga gcttcaggtc ctgggaaaac
     2641 cttcaattct gattccctcg cccaatgtcg ccgagaatca tcagtatcac aacgggggat
     2701 ccatggaaaa acgctgtgca gcgattctga ttgaggatga taaactcagc ggaaagctgc
     2761 tgatggagaa aatagaccag ttattttctc atccgggccg gcttgaaaaa atgagcaggg
     2821 cgtcgctgaa aatggctatt cccgacgcaa acgagagaat tttcaaggtt gtccgctcgg
     2881 tgttaaaaat gtaatcgtcc gaagaaatgc cctttccggc agaaccctgc cgccaggcgg
     2941 catgtacctg aacgttgcgc aaacaataag cataatatga aacaaagggc gcacagcgct
     3001 cttgtgcttt gaaagggtgc gtgacgatgt caaggctgat cgtagaaggt ggaaatcccc
     3061 tggtgggcga acagttggtt cagggagcaa aaaacagcgc attaccggtc cttacctcaa
     3121 caattctatg cgattctcca agcatgatac ataattgtcc caatttatca gatgtggacg
     3181 cttcctgccg cattttgtcc tgtttgggct gcaaagtgac cagggatgac cactcggtct
     3241 ttgttgaccc gtcggggatt cagggccacg atattcccga tgacctgatg agggagatgc
     3301 ggtcatccat tgtattcctg ggcgcgatct gtgccagaat gggcagtgcg catatttctt
     3361 ttccgggggg atgcgaactg ggccccaggc cgatagacct gcatttaaca gccctgcgga
     3421 aactcggtat acagattaaa gaagaccatg gctggctgaa ctgcagggta atcggcaagt
     3481 tgcacggagc gaatatcgtt ctgagttttc ccagtgtggg agcaactgaa aatattatgc
     3541 tcgcagcctg cacagccgaa ggggacactg tcattgtaaa cgccgcgcga gagccggaaa
     3601 tctccgacct tgccgacttt ctaaatcagt gcggagcaaa agtatccggg gcgggtgaaa
     3661 gcgttatcac gatccgtggt gttcagtctc tcaccggccg ggagcacagg gttatcccag
     3721 acagaatcgt cgccgcgacg ctgatggcgg ccgcagccat taccggaggg gaaataacgc
     3781 tccggaatgt caatccgctt cacttaagcc cggttgttcc ggtgtttgcg gaagccggct
     3841 gtgttataaa cgaatattcc gatacgatgg taatcaaacg cagcggcccg cttcgcccgg
     3901 tcaaatatgt caggacgatg ccgtatccgg gatttcccac agacgcacag gcgccggtta
     3961 tggcgatgtc cgccatcgct gcgggcacca gcatgtttat agaaaatatt tttgaaaaca
     4021 gatataaaca cgcggccgag ctgacccggc tcggagcgaa aattaacgta gaaggcaagg
     4081 tcgcggttgt cgaaggggtc cgcaggctgc ttggggcccc ggtggaagca actgatctgc
     4141 gaggaggcgc cgctctggtg gtggccgccc ttgccgctga aggaacgacc cagatttcgg
     4201 gaattaaaca tattgacagg ggctatgaaa atattgaagg cgtgctgtct gaaatcggag
     4261 ccgcgatcag gcgggaataa ccaacggtaa cccccattcg ataccattaa cgccggggat
     4321 acagaccgtc tgccgtttca agcgccgcct taaggaaggt aaacagagga tgtcggaaag
     4381 aaaacctcgc aaaacagaac cataccgagg ccgtgagctg gctccgcgta aaaaacggcg
     4441 gaggaagccg actccgtttg cctatgtggt gattctcatc acggttgcgg cctgcgcggt
     4501 atccctgtcg gctatgattt tttttaaagt tgaaaccatc cgaattaccg gaaaaaccag
     4561 gtacagcgcg cagcagattc ttgacgcagg cggggtcgga aaaggaatga atctttttgc
     4621 aatcgacaaa aagaaggctt ccggcaaaat ctgcagcgcg ttgccttata tccgttcggt
     4681 tgaaataaaa ttgatgcctc tctcaacggt tgagcttgca gtggaggagg acaagccctc
     4741 cagcatcgtt gctctgacaa gcggttacgc agtccttgat caggaatcga aggttctgga
     4801 aatcaggccg gggattaaga atctgaatct tcctatggta aagggaatga aagtctccta
     4861 cgcccagccg gggagtgttc tccgggttgc cgaacaaagt cagattcaaa tcctaagccg
     4921 gatcaggcag agcatgacac aggccaagct taaaaaagtc agcaccattg atatcacgaa
     4981 tgatctcgag attaccgcga gctatgacca gcggatcaag attgagattg gaacagcttc
     5041 cgatctcagc cagaaaatga atctcgcggc ttatgtcata gccaataaaa tatctgcttc
     5101 cgagaagggc tcccttgatg tgacagaagc ctcaaaggcc tattttgatc cgtcgtaagc
     5161 cggctcagtt gcagcggagg tcaatttatg aataagatcg cctgtctggt atcgggcctc
     5221 agaaaatatt ccggtcagag tgtcctgctg ttgatctgct ttattctcgg gttcggcctg
     5281 accatacaaa tcaaaagcgt ttaccagctc aacagcggcg ccgccagcaa cgatatcaag
     5341 cgcgctgaag atttgcagaa acgggtcagc gagcttgggc aaaaaaacaa agatctttac
     5401 aatcaggttg aaaagctcca gtcggcatta gatgattatc gggccaaagc ggccaaatcg
     5461 ggcagctatg cagacacgct caacaagcag cttcaggacg ctgagattct ggccggtctg
     5521 gccgacgttc atgggaaagg tgtcatagtg acgctcgacg atgccgagca gtccgcttca
     5581 accaatgcgt ttgtcgtgca tgactccgat atccttcagg ttctcaacga gcttcgggac
     5641 gcgggagcgg aagccctttc gatcaatgac gaaagaatcc tttccacctc tgaagtccgg
     5701 tgcgcgggga atacaatcag cgtcaatgac aaccgttatt ccgctccttt tgtgatcaag
     5761 gcaatcggca atcctgataa actcagaagt gcgattatga tgaaggacgg tattgccgat
     5821 gtgctcggcc agtggggaat caaggtcaat gtggaatcaa gcgacggcct ttacatcaaa
     5881 gcctatgagg gaatgccgaa atatcagtat gccggggcgg tcaataataa gaaataacct
     5941 gtcggccgga agggggatca tcaattgaca gttattttca gcttaatcgg cctggccgtc
     6001 ggcgtccttg cggcgctgct ctatcccggc tatattccgc agcaatatct gatttatgtg
     6061 gcggtagcaa ttcttgcggc tttggattcc attttcggcg gaatcaacgc ccgtttcaga
     6121 caaaaatttc gaaaatccat ttttatttcg ggcttttttg tcaatacggc tttggcggct
     6181 ttgctgactt ttgttggcca gctgctgcaa attgatctgt atctggcggc aattgtggtt
     6241 ttcggcacaa ggatctttca aaatttagca gaaatccgaa gatttatatt gaattattac
     6301 gccaaaaaag ataaaatatc tttataatac ctttattcta tgccagtaag caatcaaagg
     6361 acccgcgaag ggtagaaacg gccgctgcag taccggtttc aactgttccc gggcttttcg
     6421 ctgaaaaaag atcctgcttc agtgtctcgg aatgagaccc gtaggaggtc atctctcaaa
     6481 aacaaggaag tattaaattc agggaggcag aattgaatgg cttttgaact tgataaagac
     6541 ttcgacaata tagttcagat aaaagtagtt ggtgtcgggg gcggcggaaa taatgccgtt
     6601 aaccggatga tcagcacagg cgtccagggg gttgaattta tttcgatcaa taccgataag
     6661 caggcgcttt atctgtccca ggcgacgcat aaaatccaga tcggcgataa actgacccgc
     6721 ggtcagggag ccggtgccaa cccggagaag ggccagagag ccgctgagga gagccgggat
     6781 gaaattgccg atgcactcaa gggcaccaat atggtgttta tcacggccgg catgggcgga
     6841 ggtacgggaa ccggagcggc tccggttatc gcatcgattg cgaaagaact tggaatattg
     6901 accgtcggca ttgttacgaa gccgttcctg tttgaaggcc gcagaagaat ggatcaggcc
     6961 gaatccggaa taacagccct cagagaacag gtggattcgc ttgttgtgat tcccaacgag
     7021 agactgaagc tggtttccga gcagaagatt actcttgcca acgcgttctg cgtggcggat
     7081 gatgtgctgc gtcagggcgt gcagagcata tcggatctga ttaaggttcc cgggcttgtc
     7141 aaccttgatt tcgcggatgt gaccgcggtg atgaaggatg ccggctatgc gcatatgggc
     7201 gtaggccgct catccggaaa agacaaggcc gaggaagcgg cccgcatggc gatttccagc
     7261 ccgcttctgg agacctctat caacggtgcg agaggtgtga tcatcaacat tacatcttcc
     7321 gcagatatcg gtctcgaaga ggtcgaattc gcatcctcga tgattaccaa tgcggccgat
     7381 ccgtccgcaa acatcatctg gggcgcggct tttgatgaga cccttgaaga tgaaatgaat
     7441 attacggtga ttgccaccgg ttttgacagc ggtctgaatt taaacgaaac acacagcgtt
     7501 tttcaggcgg caaaagcttc ccgcgcatcc gcatccacac ccgagaacgc ttccgcccct
     7561 tcgccgaagg caaaggaaca gccgcagcat cacggcgcgc ctgctcctgc gcagcactca
     7621 caggctgacg acgagtattt tgatattatg agtatcttta accgccgaaa ataatcatga
     7681 ttgaaaacgc ggcaggtctc tatggaaata gccgtgccgc ggggtgggag tcgaatgaga
     7741 ccggtcaaat acataataat cgtaagcgcc ctgtttgcgc tggtctgtct gctgacctcc
     7801 tgtgccgcct cgcagcagaa aatcatttgg ggtatcgaca gcaattccgt tccgctgacc
     7861 ttccatgacg aggatgggtc cctttctgga
//
LOCUS       DATALY010000146         7409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_808116,
            whole genome shotgun sequence.
ACCESSION   DATALY010000146 DATALY010000000
VERSION     DATALY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808116"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VHR42_04275"
     CDS             complement(<1..1287)
                     /locus_tag="VHR42_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026429.1"
                     /translation="MDFENLYQQVQTNQSTMNSHTANISNPHQVNYQQAGAEPAGSVS
                     AHNNDSNAHGSLFVKNVTGSGAVLVTRNGNNVNVSVTGAGGGDMLTANYANGSGSSNA
                     NSVDRAKYSDDVSISKSTTLNNSSSAFQTGTSDTNPAPIALVDSSSVALAFNGRTLSN
                     VITNGNFATSSNNLATGFTLSSASAVSCALNAQTFIATAQNGLILSPVQGVTNHRYFI
                     IASVKATSNQVALSLQNATLTVPYHPGDGNYHTLYAIATLGSTAGYPYIQDRGTSGWQ
                     NISVQNFMAIDMGTDATNPYYNYTKDQMNNLINGYIDGTKSIVNPKFLSTGKNLFNSA
                     TITNGYAMGASGSPFVSSGWWASDFVPVVQGKTYIFTGLGPTTYSYVCFYSASKTFLS
                     SLTNTAVISAGKMITVPAGAYYVRFAAASSAGAFQLE"
     gene            1286..1483
                     /locus_tag="VHR42_04280"
     CDS             1286..1483
                     /locus_tag="VHR42_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026430.1"
                     /translation="MGYSFEIALEARSLGLIESVLQSAVFRMFPLESLPIKDTVSRPG
                     TVTPCLIGISIVLLTGKSLSS"
     gene            1801..3123
                     /locus_tag="VHR42_04285"
     CDS             1801..3123
                     /locus_tag="VHR42_04285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX3026431.1"
                     /translation="MRSISVIGTGYVGLVTGTCLAEFGLNVYCMDVDSSKINMLKECK
                     LPIYEPGLESLVKSNMNAGRLHFTTDIKEAVTKSETIFIAVGTPPQEDGSADMHYVIS
                     AARSIAEQMDGYRVIVDKSTVPVGTGQRVKAIIQETLDHRGVSYPFDVVSNPEFLREG
                     SAVKDFTHPDRIVVGCESEKAKDIMENIYRVLYINNHPFVFTNIETAELIKYASNSFL
                     AAKIAFINEISILCEKVGANVNDVARAMGLDNRIGRFFLHAGPGFGGSCFPKDTKALL
                     TISREHDSKSDIIDAVIKANEKQKLHMVEKIDDALGGNLKGKTIAVLGLAFKPETDDI
                     RESSAIVIVKKLLENGAIVRAYDPIAMENSIKFAFAKDEIHYAKNEYDAAEGADALVI
                     VTEWNQFRSLDIDELKSKMKGRHFFDLRNIYERNYIEGNGFCYYGVGR"
     gene            3504..4385
                     /locus_tag="VHR42_04290"
     CDS             3504..4385
                     /locus_tag="VHR42_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3026432.1"
                     /translation="MSNIFLLLAAMIWGFSFVAQRLGSQYLGAFAFNGIRFGLGCLVL
                     LPPVLISLFRNRLEENFRAKNFLSIKAGIIVGVALYAASALQQLGLAETTAGKAGFIT
                     DMYIVIVPLLGVFLGHKIPSLTWVSVALSVVGLYLISVTGQFGISRGDLLVLGGAFLW
                     AAQIILISHFANNVDTLIFSLFQFLTCSVLSLITTLFTENISWGGVLGAGIPILYGGV
                     LSVGVAFTLQMFGQKHARPSHAALLMSMEAVFACLGGMLILKETLEPKGYLGCALIVF
                     AMILSQSSAFKKEPVQA"
     gene            complement(4669..5115)
                     /locus_tag="VHR42_04295"
     CDS             complement(4669..5115)
                     /locus_tag="VHR42_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3026433.1"
                     /translation="MQKEFLPYVRLVEFLGKVLGRHTEVVLHDLTNLDQSIIAIANGH
                     ISGRKVGAPATDLALRIVSNEALKQKDYLCKYTGYAKNGYKLCSSTFFIRDGSERLIG
                     MLCINTDSERLEQARDILDSMLSDRDCCCQNGATEDISEKLPLNAE"
     gene            complement(5133..5915)
                     /locus_tag="VHR42_04300"
     CDS             complement(5133..5915)
                     /locus_tag="VHR42_04300"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX3026434.1"
                     /translation="MLVRKMTLIYFSPTGNVAKTVRSIADGTGMGWDEIDLTRFEARW
                     GKYQFGPDELVVFGFPVYGGRIPAIASECLRCIEGSSTPAVFVVSYGNRDYEDALFEL
                     SRTFEPKGFQTIASAAFIGEHSICETIAKGRPDNSDRKVAFDFGRSVIQKLESVFASE
                     QLSRVVPAGHYPYRAYSESSEPPETDENCVACGLCAKECPMQAVNPENPGETDAIRCL
                     KCCRCIHLCPQSARHFRREKVERISNMLTEKASARKEPILLI"
     gene            complement(5929..7116)
                     /locus_tag="VHR42_04305"
     CDS             complement(5929..7116)
                     /locus_tag="VHR42_04305"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HEX3026435.1"
                     /translation="MKYDFDRFIDRSGTNSVKWEFMKDNHPDVPSGTIPMWIADMDFP
                     CARPILDALHDRVDKEIFGYSSNETEKYYRAVCGWYQHRFGWFVSSRDVFFGPGVVPS
                     IGYLIALLTAPGDGVVIQPPVYYPFARMIRTHGRTIVENPLCERDGYYTMDYDDLEKK
                     VSAPNTHLMILCSPHNPVGRVWKEEELKKVVEICKRHDVNIISDEIHFDFVRQGHRHL
                     PLSKVCPEYKDHIFTATAPSKTFNLAGMQLSNIVIHNSEVQRRWSDYIFGLVGVRRPT
                     ALAVTAAQAAYEQCEEWVEQVNCYIDANMDLISQFLKDHLPKARFYLPEGTYLVWVNL
                     SGYGYDHERLDQMLLMQGRVLTEDGILFGSQGEGYIRLNAACPRQTLEESLRRISGVL
                     NKA"
     gene            complement(7152..>7409)
                     /locus_tag="VHR42_04310"
     CDS             complement(7152..>7409)
                     /locus_tag="VHR42_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX3026436.1"
                     /translation="SLSGETIEKQARQSLSNLKAVLDAAGLGMKSVVKTTVYLSDMNH
                     FSKMNEIYSEFFTGDYPARVAVEVSRLPKDVLVEIEAIAVR"
BASE COUNT         1902 a         1801 c         1652 g         2054 t
ORIGIN      
        1 ttcgagttga aaagctcccg cagatgatgc ggccgcaaat ctcacataat atgctccagc
       61 cggaacggta atcattttcc cggctgaaat tacggcagta ttcgttaaac ttgatagaaa
      121 cgttttgctt gccgaataaa agcagacgta ggaatacgtt gttggcccta accctgtaaa
      181 tatataggtt ttgccttgga caaccggtac gaaatcggac gcccaccacc cggaagacac
      241 aaacgggctt ccactcgccc ccattgcata cccatttgtt atagttgcgg aattgaacag
      301 gtttttaccg gtactcaaaa atttagggtt tacaatagat ttcgttccat cgatataacc
      361 gtttatgagg ttattcattt ggtctttggt ataattgtaa taaggatttg tggcatcggt
      421 gcccatatca attgccatga agttttgaac agaaatattt tgccatccag aggttcctct
      481 atcttgaata tatggatatc ctgctgtact tcctaaagtt gcaatagcat ataacgtatg
      541 ataatttcca tctccgggat ggtacggaac tgtgagggtt gcattttgta gggacaacgc
      601 cacctgattt gatgttgctt taacgcttgc aataataaag taacgatggt ttgtaacacc
      661 ttgaaccggc gataagatca gtccattctg tgctgttgca ataaacgttt gagcatttaa
      721 ggcacacgat actgccgatg cgcttgataa agtaaaacca gttgccaaat tatttgatga
      781 agttgcgaaa tttccgtttg tgataacatt tgataaagtt ctgccgttaa acgccagcgc
      841 aacgctcgaa ctgtcaacca aagcgatcgg agccggattt gtgtccgatg ttccggtttg
      901 aaaggccgac gaggaattat taagggtagt tgatttactg attgaaacat catcggaata
      961 tttcgcacgg tcaacagaat ttgcattgga acttccggag ccgtttgcat aattggcggt
     1021 cagcatgtct ccgccgccgg caccggtaac actgacattt acattattcc catttcttgt
     1081 aacaagcacc gctccggaac cggtaacgtt tttcacgaat aaagacccat gcgcgtttga
     1141 atcgttattg tgcgccgaaa cgcttccggc cggttccgca cccgcctgct gataattcac
     1201 ctgatgcgga ttgctgatat ttgcagtatg agaattcatg gtgctctggt tggtttgcac
     1261 ctgttgataa aggttttcaa aatccatggg gtattccttt gaaattgcat tggaagcaag
     1321 atcgcttggg ctgatcgaaa gcgttttgca atctgcggtt tttcggatgt ttccgttgga
     1381 atcgcttccg atcaaagata ccgtgagccg tcccggaacc gtgacgccct gcctgatcgg
     1441 catatcaatc gtgctactga cgggcaaatc gctttcgtca taggctccgt tcagcgcaac
     1501 aatgcgcagc cggcaggtta agtcttgcca ttcgggaggg acggggcaac gaagccatac
     1561 cgccccgttt tctccctgaa caccggcgtc cgtggtcagc gctccattgg ataaatccgc
     1621 ggcactcaga aggtttcccg ttactgtcag aagaatcgtc ttgtttttta ctggcataat
     1681 cttcaccttt ccccatacgt tgtatttctt atcacaaaaa tccgatttgc cgatattgac
     1741 atttcactct cagagtttca taattatttg tggattgcac aagaaaggaa tgtaaaatag
     1801 atgagaagta tatctgtaat cggaaccggt tatgttggtt tagttaccgg cacctgcctt
     1861 gcggaattcg gtctgaatgt atattgcatg gatgttgaca gctcaaagat caatatgctc
     1921 aaagagtgta agctgccaat ttatgaacct ggtctcgaga gccttgttaa aagcaatatg
     1981 aatgccggaa gacttcattt taccaccgac atcaaagaag ctgttacaaa gtccgaaacc
     2041 atttttatcg cagttgggac gcctcctcag gaagacggct cggcggacat gcactatgta
     2101 atctcagccg cccgctcaat tgccgagcag atggacggtt atcgggttat tgtcgataaa
     2161 agcacggttc ctgttggaac aggccaacgg gttaaggcga ttattcagga gactctggat
     2221 catcgcggtg tttcctatcc cttcgacgta gtgtccaatc ccgagttctt acgggaaggt
     2281 tccgccgtaa aagactttac gcaccctgat cgcattgtcg tgggctgtga aagcgagaaa
     2341 gcgaaagata tcatggagaa catttatcgt gtcctctata tcaacaatca cccgttcgtc
     2401 ttcacaaaca ttgaaacagc agagttaatc aaatacgcct ccaactcatt tctcgcggct
     2461 aaaatcgcgt ttatcaatga aatatcgatc ctttgtgaaa aagtcggcgc caacgtcaac
     2521 gatgttgccc gggccatggg gctcgacaat cgtatcggaa ggtttttcct ccacgcaggg
     2581 ccgggctttg gtggcagttg tttccccaag gataccaaag cactgctgac catcagccgc
     2641 gaacatgaca gcaagagcga tattatcgac gcggttataa aggccaacga gaagcagaaa
     2701 cttcatatgg tggaaaaaat cgatgacgca ctcggcggca atctgaaagg aaaaacaata
     2761 gccgttttgg gccttgcctt taaaccggaa accgatgata tccgcgagtc ttccgcaatc
     2821 gtcattgtga aaaagctgct tgaaaacggg gcgatcgttc gtgcatatga tcccatagcg
     2881 atggaaaatt caatcaaatt tgcttttgca aaagatgaaa tccattatgc caaaaacgaa
     2941 tacgacgccg cagaaggagc cgatgcactg gtcattgtaa cagagtggaa tcagttccgc
     3001 agcctggata ttgacgagct gaaatccaaa atgaaaggcc gtcatttctt tgacctgcgt
     3061 aacatttatg aaagaaatta tattgaaggc aatggttttt gctattatgg ggtcggcaga
     3121 taaaaaatct atcggctgat cattaccctt ccattgacgt ggccaagctc ttatggaaag
     3181 acttttgtga gaaggttgcc ggataacgaa ccggatcaat tcaagcttat cattcctatg
     3241 catgataagc ttgaattttt aacgcttaca ttccgggata aatgaccgat ctgtttttcc
     3301 cactttctat tggagaaccg gccgccaatt tggcagcagc cttatacccg atttattctt
     3361 catatttttc aatgttcgtt tcatttcgaa caccgttcat aaaatgaaat cactcttttt
     3421 tgcagttttc aaagaaacat ctgtgttgaa gctgtcagaa gctactactg ataaggaacg
     3481 aaataatgaa aaaacaggaa atcatgtcta acatttttct tctcttagcc gcaatgattt
     3541 ggggattttc ttttgtcgcc cagcggttag gctcgcagta tctcggagca ttcgctttta
     3601 atggaatccg ctttgggctt ggctgccttg ttctccttcc gcccgttttg atctcactct
     3661 ttagaaacag gctggaagaa aatttcaggg cgaagaattt cctgtcgata aaagccggta
     3721 tcatcgtcgg ggttgccctt tacgcggcat ccgccttgca gcagctgggc cttgccgaaa
     3781 ccacggccgg aaaagcaggt ttcattaccg atatgtatat cgtaatcgtt ccgctgcttg
     3841 gtgtttttct cggccataag ataccatccc tcacatgggt cagcgtcgcg ttatccgttg
     3901 tgggcttata tctgatcagc gttacaggcc aatttgggat ttccagagga gatttattgg
     3961 ttctgggagg cgcattcctc tgggctgctc aaattattct gatcagccat tttgccaaca
     4021 atgtggacac ccttattttt tcgctgtttc aatttctgac ctgctcggtt ctcagcctga
     4081 taaccacttt gtttacggaa aacatctcct ggggcggtgt tctcggggcc gggataccga
     4141 tcctttacgg cggcgtattg tcggtcggag tcgcgttcac cctccagatg tttgggcaaa
     4201 aacacgcccg tccttcccac gcggccttat taatgagcat ggaagcggtt tttgcctgtc
     4261 tgggaggaat gctgatttta aaagagaccc tggaaccaaa aggatacctg ggctgcgccc
     4321 tgatcgtttt tgccatgatc ctttctcagt cgtctgcctt caaaaaagag ccggttcaag
     4381 cctgattaaa ggaatagaat aaaaaatccc gggtaaaacc cgggattttt ctatgccaaa
     4441 ttttattatt tcatgttaat tcgacttctt tttaatctga tttaaatagc ggtaaacgct
     4501 gggctcagat attttgagcg ttgcggcagt ttgggcgacc gaccctttta aaagaaatac
     4561 tcccgtgtca ttaagctttc tgacaatatc aattttctcc tgaggggaca ttctttccgg
     4621 atcaaccccg agattttcca ctgcttttgc aatgctctgc agcgtcagct attcggcatt
     4681 cagcggcaat ttttcggaaa tatcctctgt tgcgccgttc tgacagcagc aatcccggtc
     4741 ggaaagcatg ctgtcgagaa tatcccttgc ctgctcaagc ctttcactgt cggtattgat
     4801 acatagcata ccgatcagcc gctctgagcc gtcgcggata aagaaagttg aggaacacag
     4861 cttatagccg ttttttgcat accccgtata tttgcataaa taatcttttt gctttagcgc
     4921 ctcattcgaa acaattctca gagcaagatc cgtggccggt gcgccgactt tcctgccact
     4981 gatgtgtccg tttgcaatgg caataatcga ttggtctaaa ttagtcaggt catggagaac
     5041 cacttccgta tgacggccca gcaccttgcc aagaaattcc acaagcctga catagggcaa
     5101 aaattccttt tgcataaatt tcctccgctt ctttatatta ataatatggg ctccttgcgg
     5161 gcagacgctt tttccgtaag catattggaa atccgctcca ctttctcccg gcggaaatgc
     5221 ctcgcactct gaggacataa atggatgcac cggcagcatt tcaggcacct aattgcatcg
     5281 gtttcccccg ggttttccgg attaaccgcc tgcatcgggc attccttggc acaaaggccg
     5341 caagcaacgc agttctcatc ggtttccggg ggttcggacg attcactgta agcccggtag
     5401 ggataatgtc ctgccgggac aacgcgggat agctgctctg atgcaaagac agattccagc
     5461 ttttgaatca cagaacggcc gaaatcaaac gcaaccttgc gatccgaatt atccggtctg
     5521 cccttcgcta ttgtttcaca aatcgaatgt tccccgataa aagcggccga ggcaatggtc
     5581 tggaaacctt tcggttcaaa ggttcgcgat aattcgaaaa gagcatcctc ataatcccgg
     5641 tttccataag aaaccacaaa cactgccggg gttgaactgc cttctatgca tcttaaacat
     5701 tccgaggcga ttgccggaat tcttccgcca taaaccggga aaccgaaaac aaccagttca
     5761 tccggcccga actgatattt tccccacctg gcttcaaaac gggtcaggtc aatttcatcc
     5821 cagcccattc cggttccgtc cgcaatactt ctgactgttt tggcaacatt tcctgttgga
     5881 ctaaagtaga tcagggtcat ctttctgaca agcattatga tacctccttt acgctttgtt
     5941 gagaaccccg gaaatccgtc tgagactctc ctccaaagtt tgccggggac atgcggcgtt
     6001 cagccggata tacccctctc cctggctgcc gaacaagatt ccatcctccg ttaatacccg
     6061 gccctgcatg agcagcattt gatcgagccg ttcgtggtcg tagccatagc ccgaaaggtt
     6121 tacccataca aggtatgtgc cttccggtag ataaaaccgt gctttcggca ggtgatcttt
     6181 gagaaattga gaaatcagat ccatatttgc atcaatataa cagttgacct gttcaaccca
     6241 ttcctcacat tgctcatagg cagcctgcgc tgccgtaacc gcaagggccg taggcctccg
     6301 aactccaacc aacccgaata tataatcact ccatctacgc tggacttcac tgttatgtat
     6361 cacgatattg gataactgca ttcccgccag attaaaggtt ttgctcggcg cggtcgccgt
     6421 gaaaatatga tccttatatt ccgggcagac ctttgaaagc ggaaggtgcc gatgtccttg
     6481 tcttacgaaa tcaaaatgga tttcatccga gatgatgtta acatcatgtc ttttacagat
     6541 ctccactact tttttaagtt cttcttcttt ccagacacgt ccgacgggat tatgcgggct
     6601 gcataatatc atcagatggg tgttcggagc ggaaaccttt ttctcaaggt cgtcataatc
     6661 catggtataa tatccatccc gctcacaaag cggattttcc acaatcgtcc ttccgtgagt
     6721 tcggatcatg cgtgcgaacg gataataaac cggcggctga ataacaacgc cgtctcccgg
     6781 cgcggtcagg agagcaatca aatagccgat tgacgggaca actcccggac cgaaaaaaac
     6841 atcgcgcgac gagacaaacc atccgaaacg atgttgatac cacccacaca ccgcccggta
     6901 atacttttcg gtttcatttg agctgtaacc gaatatctcc ttatccacgc gatcatgaag
     6961 cgcgtccaaa ataggacggg cgcatgggaa atccatatcg gcaatccaca tggggattgt
     7021 cccgcttgga acgtccggat gattatcctt cataaattcc catttcacgc tgttggttcc
     7081 gcttcggtct ataaaacggt caaaatcgta tttcatccaa ttgtttctcc ttccagtgac
     7141 tgtttaactg cttagcgaac cgctattgct tcaatttcaa caagcacgtc cttaggcaga
     7201 cgggaaactt caaccgccac acgtgccgga taatctccgg tgaaaaattc ggaatagatt
     7261 tcattcattt tgctgaaatg attcatatcc gaaagataga ccgttgtttt cacgacgctt
     7321 ttcatcccga gaccggccgc atccaaaact gctttcagat tggaaagcga ctgtcttgcc
     7381 tgcttctcaa tcgtttcccc ggataagct
//
LOCUS       DATALY010000147         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_637444,
            whole genome shotgun sequence.
ACCESSION   DATALY010000147 DATALY010000000
VERSION     DATALY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637444"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VHR42_04315"
     CDS             <1..631
                     /locus_tag="VHR42_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026437.1"
                     /translation="PTGTRFALAGRTWETLEVNQKGKVIFVKLVPGVSTVDWDIDFNS
                     ELHTVLVRKMRSILLSDDDYPYLTDSCRERLAEIREIARNSSFLSDRVVKLSENKFAV
                     FPWIGTRQLFTLHFLLLQKGIKSKIPWRTSVYLEVVFNGSVPDAKEALEEMIDGISSE
                     EADPFSLPLPHKVQIENKYNEFIPPDLLKKQFLFDFLDFGGLKESLHNP"
     gene            878..2665
                     /locus_tag="VHR42_04320"
     CDS             878..2665
                     /locus_tag="VHR42_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026438.1"
                     /translation="MLRNMKIGKKLLMTFLFVTILSSIAGIFGFASMSSVCSQYSRAL
                     SDYGFSQGDIGRFSAEFNNNGALTRDMIIQTDNAGMQTSKKKLDVSSENLETLYKKMG
                     PEMITAKEKNYYETIGIALKLYENYRDQAVNFSIGNQKTDAYNMLTTQCVPFSQRVKG
                     DIENLFQEKTKSGNQISLSLTGSQTTAEMVFGLVVLLAVFFSIFIAVRISRGISKPVK
                     KLTSVAEKMAEGDLSTEVNIESTDEIGQLAAALQKTCTSLRTYIKDIDINLAKMAQGD
                     LTIHTDLEFKGDFISLRNSIAGIVGSLNTMILQIRQAADQVSTGSGQVSNASQELAQG
                     AAEQASSVEELSATIMEISSEVDENAKHVSKASENVDTVTAEIAGCNQQMQHMVQAMG
                     EISDSSNQIGKIIKTIEDIAFQTNILALNAAVEAARAGEAGKGFAVVADEVRNLASKS
                     AEAAKETTKLIQNSVSQVEKGTKITNNTAESLLRVVDSAKAVSETVEDISKATAKQSS
                     AIKQVNTGVEQISNVVQTNSATAEESAAASEELSGQARTLSELVQAFKLKEEHETEPE
                     ENHSPDAETDPELEVPETPETSDVIPANA"
BASE COUNT          759 a          628 c          697 g          632 t
ORIGIN      
        1 tcccaccgga acgcgttttg cccttgccgg cagaacgtgg gaaaccctcg aggtcaatca
       61 gaaggggaaa gtcatctttg tcaagctggt tccgggcgtc tccacggtcg attgggacat
      121 tgacttcaat tcggaactgc acacggttct ggtgcggaaa atgcgaagca ttttgctgag
      181 cgacgacgac tatccctatc tcacggattc ctgcagagag cgtctggcag agatccgcga
      241 aattgcgcgg aattcaagct tcctgtcgga ccgggttgta aagctttcgg aaaacaaatt
      301 cgctgttttc ccttggatcg ggacccgtca gctttttacg cttcatttct tgttgcttca
      361 gaagggtatt aaaagcaaaa ttccttggcg caccagtgtt tatcttgagg ttgtgttcaa
      421 cggcagcgtg ccggacgcaa aggaggctct cgaagaaatg attgacggaa tctcttcaga
      481 ggaagcggac ccatttagcc tgccgctgcc gcataaggtg cagattgaga acaaatataa
      541 cgaattcatc ccacccgatt tgctgaaaaa acagtttctt ttcgattttc tggattttgg
      601 cggtttaaaa gagtctttgc ataatccgtg aagcgatcag tcattttccc ggaaaagcgg
      661 gaattttaaa cggaggttag gagctgcttt tgcggctcct ttttttttcg aatagctaca
      721 aaaaaacttt gtatttttta aatctggaaa tgtttagaac gaaaattggt atatactgag
      781 aactattaga ttttttggga gagattgcgc tatcgcccaa aaaacgccat ctgcataacc
      841 ataatatccg gatgcagaat ttgaaggagg aaacattttg ctaagaaaca tgaaaatcgg
      901 gaaaaagctg ctgatgacct ttctttttgt aaccatcctt tccagtatcg ccggtatttt
      961 tggctttgcc tctatgtcgt ccgtctgttc acaatactcc agagcactgt cagattacgg
     1021 cttttctcag ggtgatatcg ggcggttcag cgcagaattt aacaacaacg gggccctgac
     1081 ccgggacatg atcattcaaa cggataatgc cggcatgcag acaagcaaaa aaaagcttga
     1141 tgtgtccagc gaaaacctgg agactcttta taagaaaatg ggacccgaaa tgattacggc
     1201 aaaggaaaaa aattactatg aaacaatcgg tatcgcatta aagctttatg aaaactaccg
     1261 cgatcaggct gttaattttt ccatcggcaa ccaaaaaacg gacgcgtaca atatgctgac
     1321 gacacagtgt gttccgtttt cccaaagggt aaagggggac atcgaaaatc tgtttcagga
     1381 aaaaaccaag tcaggaaatc agatatcact cagtttaacc ggaagccaga caacggctga
     1441 aatggtgttc ggacttgtgg ttttgctggc agtcttcttt tccatattca tcgcggttcg
     1501 aatttcccgc ggaatcagca aaccggtcaa gaaactgacg tccgtcgcgg aaaaaatggc
     1561 ggagggggac ttgagtaccg aggtgaatat tgaaagcacc gatgaaatcg gtcagcttgc
     1621 cgccgcactt caaaaaacct gtacctctct ccgtacttat atcaaagata tcgacattaa
     1681 tcttgcgaaa atggcgcagg gggatctgac catacatacg gatctggagt tcaaagggga
     1741 tttcatcagt ctgcggaatt ccattgccgg cattgtcggt tctcttaata caatgatttt
     1801 acagatccgt caggctgctg accaggtttc caccggctcc gggcaggtct ccaacgcttc
     1861 tcaggagttg gcgcagggcg cggcggagca ggccagctcc gtggaagagc tttccgccac
     1921 cattatggaa atctcttcgg aagtggatga aaacgcgaag cacgtaagca aggccagtga
     1981 aaacgtcgat accgtgaccg ccgaaattgc aggctgcaat cagcagatgc agcatatggt
     2041 acaggctatg ggagagatca gcgattcttc caatcagatt gggaagatca ttaaaacaat
     2101 tgaggatatc gcgttccaga ccaatattct tgctctcaac gccgcggtcg aagcggctcg
     2161 cgcaggggaa gcgggtaaag gctttgcagt tgtagccgac gaggttcgca atcttgcaag
     2221 caagagcgcc gaggcggcca aagaaacaac gaagctgatt cagaattcgg tcagccaggt
     2281 ggagaaagga acgaaaatta ccaacaatac ggctgagtcc ctgctccgtg tcgtcgacag
     2341 cgcaaaggcg gtttcggaaa cggtagagga tatttctaag gcgaccgcca aacagtccag
     2401 cgcgatcaaa caggtcaaca cgggagtgga acagatttct aacgtcgtgc agaccaactc
     2461 cgctactgcg gaggagagtg ccgccgcgag cgaggaactt tccggtcagg ccaggacgct
     2521 gagcgagctt gtgcaggcct ttaaactgaa agaagagcac gagacggaac cggaggagaa
     2581 ccatagcccg gacgcggaaa ccgatccgga gctggaagtt ccggaaaccc cggagacctc
     2641 agacgtgatt cccgcaaacg cataaaagcc cgcgattctg tcggactgct ataaaatcaa
     2701 ccgttcgaat catccg
//
LOCUS       DATALY010000148         3863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_722151,
            whole genome shotgun sequence.
ACCESSION   DATALY010000148 DATALY010000000
VERSION     DATALY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722151"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHR42_04325"
     CDS             <1..255
                     /locus_tag="VHR42_04325"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026439.1"
                     /translation="DGGGNWGRYSFLGFDPLMTIQVSGGETTIVREGNERIFCFVNEI
                     KMDFNSFVGIDCNYLAFLCNTSEHFLILLSIVIFCRILPI"
     gene            308..2782
                     /locus_tag="VHR42_04330"
     CDS             308..2782
                     /locus_tag="VHR42_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026440.1"
                     /translation="MKKSNPKFKASLHWYKSYCFKIAVSLILLTASSFLFVFFEINDP
                     SKMNVQMICAFSAAIFLFFLLSMITAFRFAAKTNKVYKVLTELRRGHLDVRCGIRSGD
                     ESGRAAMELDLFSRELSENVVSAMRRITDGDVSGNELAPHDQNDQIVPVINEITSIMR
                     ELSHELESAVNSAAGGDFSFRCDCGKYRGSWGKFTQDVNSLFDTIAEPLEDIKDMISA
                     LSVNDYTKSLKKNYTGSFQTLADDVNEVKQTLLTMEEAIVRVSKGDTEKLDTFISQGK
                     KSENDFMIPSIIRLMQTVNHLESEFHRVTDTCRAGKISEAGVDIEMFEGKYKDIVSGY
                     NQALRAILEPVGEVNNALEAMAVNDFTITLDNKYEGEAGMLMEKTQTVLERFIHLQST
                     SAMIAHGDISELDNFIRIGKRSENDNLNPSFIKMMRSIKDLISETENIATAASFGNLD
                     YQSDADRFDGEFRNIMVSFNDAFSSMAKPMREISSIISEMSNGSLHNFVEGEYHGEFE
                     ILSETLNNTVKRISGLAKEMTEVLLRISNGDINIERVRPYLGDYSGISNSLNIIIDSL
                     NDLFGNISISASQVAAGSRQVSDGSQNLSRGATEQASSIEELTASVSLISEQTKKNAL
                     NAQEANKHSIEAKHNASIGNDQMKKMLDSMTDISNSSASISKIIKVIDDIAFQTNILA
                     LNAAVEAARAGQYGKGFAVVAEEVRNLAARSAKAANETTELIENSIDKINGGTSIAND
                     TAEALTNIVNQIDRVAGLVSDIAASSNEQADGITQIDKGLEVVSSVVTTNSATAEESA
                     AASEELSGQAETLHSMLLKFTLREKD"
     gene            complement(2844..3803)
                     /locus_tag="VHR42_04335"
     CDS             complement(2844..3803)
                     /locus_tag="VHR42_04335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3026441.1"
                     /translation="MDEYRSLMIGSKAPHFQANTTYGPVSFPEDYKGKWVVFFSHPGD
                     FTPVCTTEMMTFASMKSQFDQHNCMLMGLSIDSNPSHIEWSRAIEGYQWKDLKNVKMA
                     FPIVADDLGTIAKMYGMLMPMESATRTVRDVFFIDPEGVIRAILSYPLTTGRNMKEIM
                     RLLLALQAYDSTGNPTPADWEPGEDQILPVPQTMPTAGQRLDESKSGGIYCIDWYLCF
                     TRADGGMHKTAPEQAAKQIVSKPEETAMNMEGRESMKKPMARDSKTDMDGAAGMSGES
                     IMEQNLRLMNEPQNQGKNFFDDFPITRDYPFIDYSGNKNKPRR"
BASE COUNT         1065 a          950 c          890 g          958 t
ORIGIN      
        1 gatggagggg gaaactgggg gaggtattcg ttccttggtt ttgatcccct gatgacgatt
       61 caagtttcag gcggcgaaac gacaatcgtc agggaaggca atgaacggat tttttgtttt
      121 gtcaacgaaa ttaaaatgga cttcaattcc tttgttggta ttgattgtaa ttatcttgcg
      181 tttttatgca atacatcaga acatttctta attttgttat ctattgttat attttgtcga
      241 attttgccga tataacaaaa tgtagggttt ttacccaatt ctgcaattta gaaaaggagt
      301 gtcaagattg aagaaaagca acccgaaatt taaagccagc ctgcattggt ataaaagcta
      361 ttgttttaaa attgctgtct ctctgatttt gctgacggct tcatccttct tattcgtatt
      421 ctttgagata aacgatccat ccaaaatgaa tgttcagatg atctgcgcgt tttccgcagc
      481 gatcttcctg tttttcctgc tgagcatgat cactgctttc cgattcgcag ccaaaacaaa
      541 caaggtttat aaagttctca ccgagctgcg ccggggccat ctggatgtac gctgcggcat
      601 tcgttccggc gatgaatccg gcagagcagc gatggagctc gaccttttct cgcgggaact
      661 cagcgaaaac gtcgtttcag caatgcggag aattactgac ggagacgtct cgggcaatga
      721 gctggcgcct catgatcaaa atgatcagat cgttcccgta atcaatgaaa taacctccat
      781 catgagagag ctttcccatg aactggagtc cgctgtcaat tccgccgccg gcggtgattt
      841 ttctttccgc tgcgactgcg gcaaatatcg cggaagctgg ggcaaattta cacaggacgt
      901 caacagcctg tttgatacaa tcgcagagcc tctggaagac atcaaggata tgatttccgc
      961 tctgagcgtg aatgactata ccaaatcact gaagaaaaat tacaccggtt cctttcaaac
     1021 tcttgccgat gacgtcaatg aagtaaaaca gacgcttctt acgatggaag aagccattgt
     1081 gagggtttca aaaggcgata ccgaaaaact tgatacattt ataagccagg gcaaaaagtc
     1141 tgaaaatgac tttatgattc catccattat ccggctgatg caaacggtaa atcatctgga
     1201 aagcgaattt catcgcgtta ccgatacctg tcgggccgga aaaatcagtg aagccggcgt
     1261 tgacatcgaa atgtttgaag gaaaatataa agatatcgtt tccggttata accaggcgct
     1321 cagagcgatt ctcgagccgg taggcgaagt aaacaacgct ttggaagcga tggccgtcaa
     1381 cgactttacc atcacacttg acaacaaata cgaaggcgaa gcgggaatgc tgatggaaaa
     1441 aacacaaacg gtgctggaac ggtttatcca tctgcagagc acttcggcta tgattgccca
     1501 cggcgatatc agcgagctgg ataacttcat ccgtatcggc aaacggagtg aaaacgacaa
     1561 cctgaatcct tccttcataa aaatgatgcg gtcaattaaa gacctcatct cggaaaccga
     1621 aaatatcgcg acggcggcat ccttcggcaa cctggattat caaagcgacg cggatcggtt
     1681 cgacggggaa ttccgcaata ttatggtatc ctttaacgac gcattttcct ccatggcaaa
     1741 gccgatgaga gagatttcct caattatcag cgaaatgtca aacggcagcc ttcacaattt
     1801 tgtggagggt gaatatcacg gtgaattcga gattctttcc gaaacactga ataataccgt
     1861 taagcgcatc agcggtctgg caaaagaaat gaccgaagtc ctgctccgga tttcaaacgg
     1921 cgatatcaac attgaacggg taaggccgta tctcggcgac tattcgggaa tttcaaattc
     1981 tctgaacatc attatcgact cgctcaacga cctcttcggc aacatcagca tttcggcttc
     2041 ccaggttgcc gccggctcac gtcaggtttc cgacggcagc cagaaccttt cccgcggcgc
     2101 caccgagcag gccagctcaa ttgaggagct tacggcctcg gtttcactga tatccgagca
     2161 gaccaaaaag aacgcgctaa atgcgcagga agccaacaaa cactccatag aagcaaagca
     2221 caacgccagt atcgggaacg accagatgaa aaaaatgctt gattccatga cggatatcag
     2281 caattcttcg gcaagcattt caaaaatcat caaggtgatc gacgacattg cgttccaaac
     2341 caatattctc gcgcttaacg ccgcggtcga ggccgcccgc gccggtcaat acggcaaagg
     2401 tttcgccgtc gtagcggaag aggtaagaaa cctcgccgca agaagcgcaa aagcggcgaa
     2461 tgaaacaacg gagctcattg aaaattctat tgataagatc aacggcggaa ccagtatcgc
     2521 gaacgacacc gccgaagcgc tgacgaatat tgtcaatcag atcgaccggg ttgccggact
     2581 ggtatcggat atcgccgctt cctcaaacga gcaggctgac ggcatcaccc agatcgacaa
     2641 aggcctcgaa gtggtatcaa gtgttgtcac gaccaactct gccactgccg aagagagcgc
     2701 ggcggccagc gaggaactgt ccggccaggc cgaaaccctt cacagcatgc tgttgaagtt
     2761 tacattacga gaaaaagatt agattccttt ctgattgttc tttccctttc ctgttaaatt
     2821 cctttccaaa agacaagtcc cgcctaacgg cggggcttgt ttttatttcc ggagtaatca
     2881 ataaacggat aatcacgggt aatggggaaa tcatcaaaaa agttcttccc ctgattttga
     2941 ggctcattca ttaacctgag gttctgctcc atgatacttt caccgctcat acccgccgcg
     3001 ccgtccatat cggttttgga atcacgggcc atgggtttct tcatactttc acggccctcc
     3061 atattcatgg ccgtctcttc cggcttggaa acaatctgct tagccgcctg ctcgggcgcc
     3121 gttttgtgca ttccgccgtc ggcacgcgta aaacagagat accaatcgat gcagtaaata
     3181 ccgcctgatt tgctctcatc aagtctctgc cctgccgtcg gcattgtctg aggaacggga
     3241 agaatctgat cctcgcccgg ttcccagtcg gccggcgtcg gatttccggt gctgtcatag
     3301 gcctgaagag caagcagaag ccgcatgatc tccttcatgt ttcttccggt cgtcagagga
     3361 taagaaagga tcgcccggat aacgccttca ggatcaatga aaaacacatc acggactgtc
     3421 cttgtggctg actccatcgg catcagcatc ccgtacattt tcgcgatggt tccaagatca
     3481 tcggcgacaa tcgggaatgc cattttaacg tttttaagat ctttccactg atagccttca
     3541 attgccctcg accattcaat atgggaagga ttcgagtcga ttgaaaggcc catcaacatg
     3601 cagttatgct ggtcaaactg acttttcatt gacgcaaagg tcatcatttc agtcgtgcag
     3661 accggagtaa aatctcccgg atgactaaaa aaaacgaccc attttccctt gtaatcttcc
     3721 ggaaaactaa cgggaccata tgtagtattg gcctgaaaat gcggcgcttt gctcccaatc
     3781 atcagcgacc gatactcatc catcggcaaa acccccaaaa gtaaaattca catcagtatg
     3841 atggttcact tttcagtgta tgc
//
LOCUS       DATALY010000149        11704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_808771,
            whole genome shotgun sequence.
ACCESSION   DATALY010000149 DATALY010000000
VERSION     DATALY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808771"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1177
                     /locus_tag="VHR42_04340"
     CDS             242..1177
                     /locus_tag="VHR42_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX3026442.1"
                     /translation="MKRHKFGIVIILAAVIVITGTACKSNQTTLASTQVSSASSSIDL
                     SNALRDSFSGAASSETPSTVAVPSSATVIVPVMGISLSKTKVSLEAGGHTMPIVTMLP
                     KSATNKGEIWESSNTKVATVNKYGSITAIHVGSCTVTVTSASNRSVSQKVSVTVTPKP
                     AEEPVQIAKPVINTAMDARAQNFGSSTSYLILVDLANQKVGIYRGSKGSWNLSQSYIC
                     SSGVGGDDATPTGTFTIHSRGTWFFSRSEQMGAEWWTQFSGDYLFHSLPMDQNRNVVD
                     ATLGKPASHGCIRLSIENAKWIYNNVPRGTTVNIY"
     gene            1358..1434
                     /locus_tag="VHR42_04345"
     tRNA            1358..1434
                     /locus_tag="VHR42_04345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1392..1394,aa:Pro,seq:tgg)
     gene            1518..1593
                     /locus_tag="VHR42_04350"
     tRNA            1518..1593
                     /locus_tag="VHR42_04350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1551..1553,aa:Thr,seq:tgt)
     gene            1608..1692
                     /locus_tag="VHR42_04355"
     tRNA            1608..1692
                     /locus_tag="VHR42_04355"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Tyr,seq:gta)
     gene            1816..2736
                     /locus_tag="VHR42_04360"
     CDS             1816..2736
                     /locus_tag="VHR42_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX3026443.1"
                     /translation="MNLRANRNHSASKTKKRAERRGDFALFFFAFFICILFFGILGFA
                     FSRSFGSSQKSSDLQTASETFTTEFSEKDSRNILFVICGDDSQSVSECVLIRMDAVKK
                     KIAVLPIPPESEMKQGDHSSSFCALFRSSGINLAKTALAQRASVAIDFYCVLKSSDYI
                     RMIDSLGGVIYDVRSPVTADISGTGAITVTKGRQNLSGTKSLALISDPEMKTDASRAL
                     LIQAELVKSFLGQKMTGYYLESATDLYKNVFDSAATDFSMNDLLDSMKLYQLITRKAD
                     SFVQLAPVFSTAAQDDFFNSYKKVLQQYYK"
     gene            2990..3418
                     /gene="rplM"
                     /locus_tag="VHR42_04365"
     CDS             2990..3418
                     /gene="rplM"
                     /locus_tag="VHR42_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX3026444.1"
                     /translation="MPTFMPKAENISRKWYVLDAAGKPIGRVATVAASLLRGKHKPDF
                     VTHLDFGDHVIILNAEKAVLTGNKLDQKYYRHHTAYIGGLKEIKYRTLMSKNPEKAIE
                     LAVKGMIPNTVIGRSALGRLRVYRGAEHIHAAQKPETWEL"
     gene            3437..3835
                     /gene="rpsI"
                     /locus_tag="VHR42_04370"
     CDS             3437..3835
                     /gene="rpsI"
                     /locus_tag="VHR42_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX3026445.1"
                     /translation="MYNDKPYFYGTGRRKKSIARVRLYPGTGSITINGRDIDDYFGLE
                     TLKLIVRQPMVVSNTLGRFDIIAKVSGGGVSGQAGAIRHGLSRALLQTGEDLRGVLKK
                     AGFLTRDPRMKERKKYGLTAARRAPQFSKR"
     gene            4267..5235
                     /locus_tag="VHR42_04375"
     CDS             4267..5235
                     /locus_tag="VHR42_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX3026446.1"
                     /translation="MIIIAAIVLNTKLVQVIDGSGEIWVYTTRSRPASILKQCGIDLS
                     PGDEYRFSGFKDNFARINLLEAFPVSITADQKTREVLLAKGTVADALDKAGVTLSPDD
                     QINQPFSKAVVAGMKIKITRVSYQTVTEKRVLPFGVSTSTSPLLKKGSSLLKVRGHNG
                     QETVVKKIKYIDGKAVGATLLRRVVDLSPTNSQVLVGSSASTPVSVLSPPSWLTLSNR
                     GTPDRYVNVISAVATAYTAGKGAGTASGRRAMAGHVAVDPRVIPYGTRLYIMSSDGDF
                     VYGYAIAADTGEFVYNGSGVDVDLYFNTMAECRKFGKRTVSIYVLG"
     gene            5478..7460
                     /gene="metG"
                     /locus_tag="VHR42_04380"
     CDS             5478..7460
                     /gene="metG"
                     /locus_tag="VHR42_04380"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX3026447.1"
                     /translation="MHCHNQEDNSMNKKKYYITTPIYYTSGKLHIGHSYTTVAADAMA
                     RFRRMQGYDVMFLTGTDEHGQKIEAKANAAGISPKEYVDAIVSDIKKLWGTLNISYDR
                     FIRTTDDYHEKSVQKIFKALYDKGDIYKGKYKGWYCSPCEAFWTENQLKDGKCPDCGR
                     EVKWAEEEAYFFRLSNYADRLIEYYQQNPGFIEPQSRLNEMVNNFIKPGLEDTCVSRT
                     SFKWGIPVDFDPKHVIYVWIDALTNYITALGYGSADDSDYKKYWPADVHLVGKEIVRF
                     HTIIWPAILMALDLPLPKKVFGHGWLLLGGDKMSKSKGNVVDPIVLCDKYSVDAIRYF
                     LLREIPFGSDSTFSLEALVGRINSDLANDLGNLVSRTIAMAEKYFGGVLPSERESAEI
                     DSELAKLSTEVFAAIEDNMDRLQYSNALTEAWKLVSRTNKYIDETMPWALAKDDARKA
                     RLAAVLYNLCESIRIISIFIEPFMPDTAAAIRRQIGVTPDVSTWDSAKVFGALPDGIQ
                     VTKAPALFPRLDYEQEKASFENASAENKAETKTASQAPKIPEGIAQIGIEDFAKVELR
                     VAKIELCEPIKKSKKLLRFQLEIAGESRQVVSGIAKWYSPQELIGKKVILVYNLKPAK
                     LCGVESNGMILAADVSEDDVKVLFVDQDIPTGSKVR"
     gene            7474..8250
                     /locus_tag="VHR42_04385"
     CDS             7474..8250
                     /locus_tag="VHR42_04385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX3026448.1"
                     /translation="MELFDSHSHYYDEAFDADRDQLLSSLPDSGVCGIINAGTDAASS
                     LSCVRLAKQYPFAYAAAGIHPESAETSENKDLESIIRLIDSEDKVVALGEIGLDYHYP
                     IDRDRQKYWFENQLALSVEKNIPVIIHDREAHGDTLELLKKYRPKGVLHCFSGSAEMA
                     EEVLGLGIYLGFTGSVTFKNNRKAARVLSITPLSRILIETDCPYMAPEPLRGRRCDSS
                     MLRHTLGYIAGVKGLEPETAAAATAANARELFQIDRRQEI"
     gene            complement(8347..9171)
                     /locus_tag="VHR42_04390"
     CDS             complement(8347..9171)
                     /locus_tag="VHR42_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3026449.1"
                     /translation="MEGFDSKDCNEDTSEKIMSNFSNEYRMPLTIIFSTLGLIARRLS
                     EKQDDTMKAYLKLVTQNCYRLLRLSNNMTEIMKYRAGLNMLHLKNGDICQFTEGLCKA
                     AGILTGAAGVKLSYHLPKQNIITAFDPAKLSVVFFNLFSNSCKYTQEGNQIKVKLEQR
                     DRQVVITIADQGMGVSNDIIDRIFEPYFSFNPNGNPFGGAGLGLTLARYIVLQHGGTI
                     AVKSREGEGTTVAFVLPIRQVGDIPDYTAEKSVDYLSDRFSMLYVELSDVCGCPMP"
     gene            9674..10693
                     /gene="gap"
                     /locus_tag="VHR42_04395"
     CDS             9674..10693
                     /gene="gap"
                     /locus_tag="VHR42_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX3026450.1"
                     /translation="MGIKIGINGFGRIGRLVFRAAMESPDLYEVVGINDPFVDADYMV
                     YMVKYDTMHGKFNGCVSTKDGKLVVNGVKIEVYAEKDPAMIPWKACGADYVVESTGVF
                     TSTEKASAHLAAGAKKVVISAPAKDKDTPTFVCGVNLEKYTKDMKVVSNASCTTNCLA
                     PLTKVINDKFGIVEGLMTTVHSTTATQKTVDGPSKKDWRGGRAAAGNIIPSSTGAAKA
                     CALVIPEVKGKLTGMSFRVPTLDVSVVDLTVRLEKSTTYEDICAAVKEASETSMKGIL
                     GYTDEDVVSSDFLTDPRTSIFDAKAGIMLNPNFVKLVSWYDNEWGYSNKVLELIKHMA
                     KVDAE"
     gene            complement(10805..11653)
                     /locus_tag="VHR42_04400"
     CDS             complement(10805..11653)
                     /locus_tag="VHR42_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEX3026451.1"
                     /translation="MSKIRQKAKASGLSLLKGKWLQAGVIILLILILEMGISALDDAY
                     RRAFNVPLDYDQIMLGQQINLSTASFIIALVFSLLTLLLVTPLEFGQKEWFWNLAENK
                     EPPISRVFNWFGSFRLYGKSVLLNLNIAVRSALWTILICGVPFAMIFGAYYMEIHYSD
                     SKYLMISLALSLFGLLLLLGAVFLLIYVLTRYFLAVYLFVEDNTRKTNGVIKESIRIS
                     KGYRMEILKFTLSFIGWFFLRQLSYGLLSFYVDPYYYSSSVIFSKHLIYTKRAADKAD
                     VAQNTD"
BASE COUNT         3271 a         2789 c         2660 g         2984 t
ORIGIN      
        1 ggagatatta agtaaccaat ttcacaatca aatacataga gtaagggttc agcttatatg
       61 ctgaaccctt tttctgttga ttacgatatc aaatgattcc agaatttata ataaaaaaga
      121 gtaaaaaata atccattgaa agatactttc tgtattacta taatttaaaa ttgcacgacc
      181 ctgaaacgtt tgtaataatt ataaaaatca tctcagctac cattataaaa gaggattaag
      241 aatgaaacgc cacaaattcg gaatcgtaat cattttagct gccgtaatag ttataacagg
      301 tacggcctgc aagagtaatc aaacaacttt ggcgagtaca caagtaagtt cggcatccag
      361 cagcatcgat ttaagcaacg cgttaaggga ttctttcagc ggtgcagcat cttcagaaac
      421 accaagcaca gtagcggtgc catcatctgc aactgtgatt gttccggtta tgggaatttc
      481 cttatcgaaa acaaaagtat cactagaggc aggcggccat acgatgccga tcgtaacaat
      541 gttgcctaag agtgcaacaa ataaaggcga gatttgggaa tcctcgaata caaaagtagc
      601 aacggtaaat aaatatggca gcataacggc gattcatgtg ggatcctgta cggtaacagt
      661 aacttctgca tcgaacagaa gtgtttctca aaaggtaagt gttacggtta caccgaagcc
      721 ggcagaagaa ccagtgcaaa ttgcaaaacc tgtaatcaac actgcgatgg atgccagagc
      781 acaaaatttt ggcagcagta caagctattt gattcttgta gacttagcaa atcaaaaggt
      841 aggaatctat cgtggcagca agggaagttg gaatttgtca cagagttata tatgctccag
      901 cggcgttggt ggagatgatg caactcccac cggaacattt acaatacata gccgaggcac
      961 atggtttttt agccgatctg agcaaatggg cgcagagtgg tggacacagt tcagcggaga
     1021 ttatctgttt cactcgctgc caatggacca aaaccggaat gttgtagatg ccactttagg
     1081 caagccagcg tcacatggct gtattcgttt atcaattgaa aatgcaaaat ggatctataa
     1141 taatgtgccg agaggtacta ctgtgaatat ttattgagag tatctaagct gatcatttta
     1201 attatgttca gcttatttca ataaaaacac ttgacttttc ttggagtatt acatataata
     1261 aataagctgt ccgatgacgc gggcacaagt taattgccac atagtaaagc cattagccgc
     1321 acagcagcgg aactttagct gtgcaaatca tcaacaacga ggtgtaactc agcttggtag
     1381 agtgcttggt ttgggaccaa gatgccgcag gttcaagtcc tgtcgcctcg accagcataa
     1441 aaatacttaa aaaatcctga cgaattccta ttgacaaagg aacgaaacct gtggtattat
     1501 gttcaagcga ttccaatgct ggtgtagctc agctggtaga gcagctgatt tgtaatcagc
     1561 aggtcggggg ttcaaatccg tccaccagct ccactttaat ttcatatggg gggattcccg
     1621 agtggccaaa gggggcagac tgtaaatctg ttgtcagtga cttcggtggt ccgaatccac
     1681 ctccccccac caaaagccgt gcctttgagg cacggctttt ttcgttttac ggatgttttt
     1741 aaatatttca ccaataataa atcataattt gaatgtaaaa atcaaataat aacagtctgg
     1801 aaaaggagga ttgcaatgaa tctgagggca aacaggaacc actctgcctc caaaactaaa
     1861 aaaagagcgg aaagacgcgg cgattttgcc ctgttctttt tcgctttctt tatatgtatc
     1921 ctttttttcg gaattctcgg atttgctttt tcgcggtctt tcggatcatc gcaaaaaagc
     1981 agcgatttgc aaaccgcttc tgaaacgttt actaccgaat tttctgaaaa agacagccgg
     2041 aacatacttt ttgtcatctg cggggacgat tcccaaagtg tatcggaatg cgtcctgatc
     2101 cggatggatg ccgtcaaaaa gaagatcgcc gtcttgccga ttccgccgga gtctgaaatg
     2161 aaacagggcg atcactcctc ctcattctgc gccttgtttc gcagttccgg aattaatctg
     2221 gcaaaaaccg ctcttgcgca acgagcatcg gttgcgattg atttttactg cgtgttgaaa
     2281 agcagtgatt atatacgtat gattgatagc ctcggcggtg tgatttatga cgtacggtcc
     2341 cctgtgacag ccgatatttc gggaacagga gcaattaccg tcacaaaagg ccggcaaaac
     2401 ctcagcggca caaaatcact cgccttaatt tccgaccccg aaatgaaaac cgatgcttcc
     2461 cgcgcgcttc tcatacaagc cgaacttgtt aagagttttc tggggcagaa aatgaccgga
     2521 tattatctgg agagcgcaac tgatctttat aagaacgttt tcgactccgc cgcgaccgac
     2581 ttctcgatga acgaccttct cgacagtatg aaactttacc agcttatcac caggaaagcc
     2641 gacagctttg tgcagcttgc gcctgttttc agcacagccg cacaggatga tttttttaat
     2701 tcctataaaa aggttcttca gcagtattat aaatgaagca aacgatgtcc gcttccccgg
     2761 gccacaattc gggttttaag ccgacatcac cgtgaaaaat gtaaatagcg ggtcagccac
     2821 ggcccataat aaagtgaaaa caggagcaat tggcagataa taagatgtaa attaaaataa
     2881 tcgttaattt atattgacat cttgtttcat tgacattata atatacatgc agttcatatc
     2941 ggtatagcct gaaaaggctc gttgatatca taaatcggga ggaaaattta tgccaacctt
     3001 tatgccaaaa gcggagaata tttcccgcaa atggtacgta ctggatgcag ccggcaaacc
     3061 tattggccgt gttgcgacag ttgcagcctc gcttctgcgc ggcaaacaca agccggattt
     3121 cgttactcat cttgacttcg gcgaccatgt tatcatttta aatgccgaaa aagcggttct
     3181 gacgggcaat aaactcgatc agaaatatta ccggcaccac accgcttata ttggcggact
     3241 caaagagatt aaataccgca ccctcatgtc taagaatccg gaaaaagcca ttgaacttgc
     3301 tgtcaaaggc atgattccga acaccgtaat cggccgttcg gctttaggca gacttcgcgt
     3361 gtatcgcggc gctgagcata tccacgcagc tcagaagccc gaaacatggg aactttaaga
     3421 ggggaggaaa gcttttatgt ataacgataa gccttatttt tacggcacag ggcgcagaaa
     3481 aaagtccata gcccgcgtca ggctatatcc cggaacaggt tcgatcacaa ttaacggcag
     3541 agatattgat gattatttcg gccttgaaac tctcaaattg attgttcgtc agccgatggt
     3601 ggtatccaat acgctcggtc gctttgatat cattgccaaa gtgtccggcg gcggagtctc
     3661 cggccaggcc ggcgccatcc gtcacggtct ttccagagcg cttctgcaga ccggagaaga
     3721 tctcagagga gttctgaaaa aggcggggtt cctgacccgc gacccgagaa tgaaggaaag
     3781 aaagaaatac ggcctcacag ccgcacgccg cgctcctcag ttctcaaaaa gataacatca
     3841 aaatcgcagc gcttttcgac gcgctgcgat ttttttcatc tttttttgtg catattcaat
     3901 atttatactt aatattcgtt cttaatttct ttaatttaca ccaatgtaat cacttgacaa
     3961 taccctatga aatgaaatat aataaccaaa accaaattta accggaaatg tttggacgta
     4021 acccgatcct ggcacaaaaa ccggttattt tttgttgtcg gcatgttttc cgtatgccgt
     4081 ataaataaaa ataaagaaac ggataagggg caatgtaatt tgaatatcgt ggctaagctg
     4141 aaaccggttt tccggatcct gcatagccgt atgcaaagca agccatttgc cagtcttaaa
     4201 agacgcaagt ggtcttgtcc tgtgcgaacc cgcatttttt cggcatgttt atttttaagc
     4261 gcatccatga ttatcatcgc ggcaattgtt ttaaacacaa aactcgtaca ggtaattgac
     4321 ggcagcgggg aaatttgggt ctataccaca agaagccgtc cggcgtccat tctcaaacaa
     4381 tgcggtattg acctgtcccc cggtgatgaa tatcggttca gcggtttcaa agacaatttt
     4441 gcaaggatta atttactgga agccttcccg gtcagcataa cagccgacca aaaaacgcgc
     4501 gaggttctac tcgcaaaagg tactgtggcg gacgcccttg acaaggcggg tgtgacgctc
     4561 agcccggatg accagatcaa ccagccgttc agcaaagcgg tggtcgctgg tatgaaaatt
     4621 aaaatcaccc gggtcagcta ccaaacggtt acggaaaaac gggttttacc ttttggggtc
     4681 agcacaagca cttctccgct gctcaaaaaa ggcagcagcc ttctgaaggt gcgcggccac
     4741 aacgggcagg aaactgttgt gaaaaagatc aaatacatcg acgggaaagc cgttggtgcc
     4801 acactgctca ggcgcgtcgt tgatttaagc cctaccaaca gccaggtgct ggtgggcagt
     4861 tcagcaagca ctccggtgtc cgtgctgtct ccgccgtcgt ggcttacgct ttcgaatcgc
     4921 ggaactccgg atcgctatgt gaatgtcatt tctgccgttg ctaccgccta caccgcgggt
     4981 aaaggcgcag gcacggccag cggacgcagg gcaatggcag ggcatgtggc cgttgacccc
     5041 agagtgattc cctatggaac aagactttat atcatgtctt ccgacggtga ttttgtttac
     5101 ggttacgcca tagcggccga cacaggcgaa ttcgtctata acggcagcgg cgttgacgtt
     5161 gacctgtatt ttaacaccat ggccgaatgc agaaagttcg gtaagcgcac cgtcagtatt
     5221 tatgttttgg gttagcaaaa ccggccgttg gagttcctcc ggcccgatcc ccacggtcac
     5281 aagcgtgggg atcgttttgc gttatgttga attaaagtgt cattatggta taatagtatt
     5341 attgcggtca tgcaggcata ccaccggttg aaccggcaaa aaagctgacg gtgaaaacct
     5401 cttcggttca acatattcga ttttaaagtt tcctgtctgc attgcaaaaa gcccgtacca
     5461 taaaggatcc caggcaaatt cattgccata atcaggagga caacagtatg aacaaaaaaa
     5521 agtattacat caccaccccg atttattaca cctccggcaa gctccacatc ggtcacagct
     5581 acacgaccgt tgcagctgac gcgatggcaa ggtttcggcg aatgcagggt tatgatgtaa
     5641 tgtttttgac cggcaccgac gagcacggcc agaagatcga agcgaaagcc aacgccgcgg
     5701 gcatatcccc caaggaatat gtcgatgcca tcgtcagcga tattaaaaag ctatggggca
     5761 cgctcaacat ttcttacgat cgttttatcc gcaccaccga cgactatcat gaaaaatccg
     5821 ttcaaaagat tttcaaggcc ttatatgaca aaggcgatat ttataaggga aagtacaaag
     5881 gctggtattg ctcgccctgc gaagcatttt ggaccgaaaa tcagcttaag gacggtaaat
     5941 gtccggattg cggacgtgag gtcaaatggg ccgaggaaga agcatatttt ttccggcttt
     6001 ccaactatgc cgacagactg attgaatatt atcagcagaa ccccggattt attgagcccc
     6061 agtcgcggct gaacgaaatg gtaaacaatt ttattaagcc cggcctggaa gatacctgcg
     6121 tttccagaac atctttcaaa tggggcattc ctgtagattt cgaccccaaa catgttattt
     6181 atgtctggat cgacgccctg accaattata tcacggcttt gggttatgga tcggcggacg
     6241 attctgatta taaaaaatac tggccggccg acgttcacct tgtcggcaag gaaattgtac
     6301 gtttccacac cattatctgg ccggccattc ttatggcgct ggatttgccg ctccccaaaa
     6361 aagttttcgg ccacggctgg ctgcttctcg gcggcgataa aatgtcaaaa tcgaaaggca
     6421 atgtcgtcga tccgatcgtt ctgtgtgata aatattcggt tgacgccatc cgctatttcc
     6481 ttcttcggga aattcctttc ggttccgaca gcaccttctc gcttgaggct ttggtaggac
     6541 ggattaattc cgaccttgcc aacgatctgg gcaaccttgt atcccgcaca attgccatgg
     6601 ctgagaaata cttcggcggt gttctgcctt ccgagcggga aagcgccgaa atcgacagcg
     6661 agcttgcaaa gctttccact gaggtctttg ccgccattga ggataacatg gaccgtctgc
     6721 agtattccaa tgcgctcacc gaggcctgga agctcgtctc gcgcaccaat aaatatatcg
     6781 atgaaaccat gccctgggcg ctggcaaaag acgacgcgcg caaagcaagg cttgccgccg
     6841 ttctctacaa cctctgtgaa tccatccgca ttatctcaat ttttatcgag cctttcatgc
     6901 ctgataccgc agccgccata cgccgtcaga tcggcgtgac accggacgtc tccacctggg
     6961 acagcgcaaa ggtcttcggc gccctgccgg acgggattca agtaaccaaa gctccggccc
     7021 tcttccctcg tcttgattac gagcaggaaa aagcctcgtt tgaaaacgct tcggcggaaa
     7081 acaaggccga gacgaagaca gcgtctcagg ctcccaaaat tccggaaggc atcgcccaga
     7141 tcggaattga agattttgca aaagtggaac tgagggttgc taagattgaa ctatgcgagc
     7201 cgataaaaaa atcaaaaaag ctgctcagat tccagcttga aatcgcaggg gaatccagac
     7261 aggttgtttc cggaattgca aaatggtatt ctccccagga gcttatcggg aaaaaagtaa
     7321 ttctcgtcta taatctcaag ccggccaaac tgtgcggcgt tgaaagcaac ggcatgatcc
     7381 tcgccgccga cgtttccgag gatgatgtaa aagtcctgtt cgtcgaccag gacatcccta
     7441 ccggttccaa agttcgataa aaggcaggat catatggaac tttttgattc tcattcccat
     7501 tattatgacg aggcgtttga tgccgaccgg gatcagcttc tctcttctct cccagattcc
     7561 ggcgtatgcg gtattatcaa cgcaggcaca gacgctgcct caagcctcag ctgtgtgagg
     7621 ctcgcaaagc agtatccgtt cgcctatgcc gccgcgggta ttcaccctga atcggccgaa
     7681 acatcagaga acaaagatct cgaatccatc atccgcttga ttgattcgga agataaagtg
     7741 gtggcgctgg gtgaaatcgg gctggattat cattacccga ttgaccggga caggcaaaaa
     7801 tactggtttg agaatcagct cgccctttcg gttgaaaaaa acattcctgt tatcattcac
     7861 gaccgcgagg cccacggcga cacgctggaa cttttaaaaa aataccgtcc caaaggagtc
     7921 cttcattgct tttccggcag cgcggaaatg gcggaggagg ttctgggact tggcatttat
     7981 ctcggattca caggctcggt aacttttaag aacaaccgga aggcggcccg ggtactctcg
     8041 atcacgccgc tctcacgcat tctaattgaa accgactgcc cttatatggc tccggagccg
     8101 ctcagaggcc gccgctgcga ttcttctatg ctccgccaca ctttgggcta tattgccggg
     8161 gtcaaggggc tggagccgga aacagccgcc gccgcaaccg ccgccaacgc gcgggaactg
     8221 tttcaaatcg accggcggca agaaatataa gcattcccgg ggactgcggc tatgaaatta
     8281 aaaaatcacg ggcgaaaatt cgcccgtgat tttttatctg atgatagcag tcaatcgcat
     8341 gaacttctaa ggcattgggc atccgcacac gtcggaaagc tcaacataaa gcatggaaaa
     8401 gcgatccgaa agatagtcca cactcttttc cgccgtatag tcgggaatat cgccgacctg
     8461 ccggatcggc agaacaaacg caacggttgt tccctcgcct tcgcggcttt ttacggctat
     8521 ggttccgccg tgttgaagaa cgatatacct tgcaagcgtc aagccaagcc ctgcgccccc
     8581 aaacgggttc ccgttcgggt taaatgaaaa atagggctcg aaaatacggt cgatgatgtc
     8641 attgctgacc cccatgccct gatcggctat cgtgattacc acctgacggt ctctctgctc
     8701 aagttttact tttatctgat ttccttcctg cgtatatttg cagctgttgg aaaacagatt
     8761 aaagaaaacc acggaaagct ttgcaggatc aaaggcagta atgatattct gtttcggcaa
     8821 atgatagctc agcttaacac ctgcggctcc cgtcagaatt cccgcggctt tgcacagccc
     8881 ttcggtaaat tggcagatat ctccgttttt cagatgaagc atattaagcc cggcgcggta
     8941 tttcataatt tccgtcatat tgtttgaaag cctcagaagc ctgtagcagt tctgtgttac
     9001 cagcttcaga taggctttca ttgtgtcgtc ctgtttttcg cttagccgtc gggcgatcag
     9061 cccaagcgtt gaaaatatga tcgtcagcgg catccggtat tcatttgaaa aattcgacat
     9121 tattttttcg gacgtatctt cgttgcagtc tttggaatca aacccttcca atctgaaaaa
     9181 aacctgactt ccaactaagc cttcctctga tatgatggga attattctta tagcaaactc
     9241 gttaaatggt tcgacgaccg tggatgccag gaatgtcctc cccgatcgca aaacgcttat
     9301 aatcttgtca aaatcataac tgtctacaag gtcacggaat cctcctggta tcgccagtgt
     9361 cggaaacctt tttaaagctt ctgagtttgc ccagcatatg tcaagtcgat tgtctaccag
     9421 aatgatcgga tgaaccgtat ttttatataa gttttctgag acttcttcca gttccggcaa
     9481 attcaaatat ctcacttccc tcaaatatta tatcattttg tctttataag taaacctgcc
     9541 aacatgacga tcaatttcct ttctgtcatt catttgttgg gtaaattgat aaatattgaa
     9601 caatctcttg tataattctt aaaaatggta taaaatattt atcggaaata aacgtttgga
     9661 ggtttgaaaa acaatgggaa ttaaaattgg aattaacggt tttggccgca taggccgtct
     9721 tgttttccgt gccgcaatgg agagccctga tctttatgag gttgtcggaa tcaacgatcc
     9781 tttcgtcgat gcagactata tggtttacat ggtaaaatat gatacgatgc acggcaaatt
     9841 caacggctgt gtttccacca aagacggcaa acttgttgtc aacggtgtca aaatcgaagt
     9901 ttatgccgaa aaagacccgg caatgattcc gtggaaagcc tgcggcgccg attatgtcgt
     9961 tgaatccacc ggcgttttca ccagcactga gaaagcttcc gcccatctgg ctgccggagc
    10021 caaaaaagtt gtcatttccg cccctgcgaa agacaaagac accccgactt tcgtctgcgg
    10081 tgtaaatctt gagaaataca cgaaagatat gaaggttgtt tccaacgctt cctgcaccac
    10141 caactgcctt gctccgctta ccaaggtcat taatgataag ttcggcattg tcgaaggact
    10201 tatgaccaca gttcactcga ccacagctac ccagaagaca gtggacggcc cttccaagaa
    10261 agactggcgc ggcggccgcg ctgcagccgg caacatcatt ccgtcctcca caggcgctgc
    10321 gaaagcctgc gcgctcgtta ttccggaagt caaaggcaaa ctgaccggca tgtctttccg
    10381 cgttccgact cttgatgttt ctgtcgttga cctgacagtc cgtcttgaga aatccaccac
    10441 ctatgaagac atctgcgccg ctgtgaaaga ggcttccgaa acatcaatga agggtattct
    10501 gggttacacc gatgaggatg ttgtttcttc tgatttcctc accgatcccc gtacctccat
    10561 ctttgatgca aaagccggca tcatgctcaa cccgaacttt gtcaaactcg tttcgtggta
    10621 tgacaatgag tggggttatt ccaacaaagt ccttgagctg atcaaacaca tggccaaggt
    10681 cgacgccgaa taagcgtaaa ttactgatga aaaaggccgt ctctttcgag gcggcttttt
    10741 tgttgctgtt ttttgaggcc agacttatta ttactcaata ctgttaaatt atccgtgcac
    10801 ctggttagtc cgtgttctga gccacgtcag ccttgtcggc cgcccttttg gtatagatca
    10861 gatgttttga aaaaataacc gaagaggaat agtaataggg atcaacataa aacgacagca
    10921 acccgtagga cagctgcctg aggaaaaacc agccaataaa cgacagggta aatttcaaaa
    10981 tttccatccg gtatcctttt gaaattcgaa tggattcttt gattacgcca tttgtttttc
    11041 gcgtattatc ctccacaaac aaataaaccg ccaaaaagta tctggtcaaa acatagatta
    11101 aaagaaaaac cgcgcccaaa aggagtaaaa gtccaaagag ggaaagggcc agactaatca
    11161 tcaaatattt actatcactg tagtggatct ccatgtaata agcgccgaat atcattgcaa
    11221 acggaacacc gcagatcaaa atcgtccaca atgcgctcct gacggcgata ttcaggttaa
    11281 gcaggacgct tttcccatac agccgaaaag acccgaacca gttaaaaacc cttgagatgg
    11341 ggggttcctt attttccgca agattccaga accactcttt ttgcccaaac tccagcggag
    11401 tgaccaaaag cagcgtcagc agtgaaaaaa caagcgcgat gataaaagaa gcggtggaaa
    11461 gattaatttg ctggccaagc ataatctgat cataatccaa aggaacatta aacgcccgac
    11521 gataagcgtc atcaagcgcc gaaataccca tttccaggat caggatcaat aaaatgataa
    11581 cgcctgcctg caaccatttt ccctttagca gggacagccc gcttgctttc gccttctgtc
    11641 tgatttttga cattcgatca actccagtta atgctattga gcctatttta ctattaatag
    11701 cctg
//
LOCUS       DATALY010000150         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_294133,
            whole genome shotgun sequence.
ACCESSION   DATALY010000150 DATALY010000000
VERSION     DATALY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294133"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /gene="ssrA"
                     /locus_tag="VHR42_04405"
     tmRNA           <1..135
                     /gene="ssrA"
                     /locus_tag="VHR42_04405"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(96..1631)
                     /locus_tag="VHR42_04410"
     CDS             complement(96..1631)
                     /locus_tag="VHR42_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3026452.1"
                     /translation="MQIAVAYARYSSDNQRQESIDAQVRAITEYCRKNQILLAHVYKD
                     EAVSATTDHREGFLQMIEDAKGGGFQLCIVHKLDRFARNRYDSAFYRKSLEKCGVKVV
                     SVLEPLDNSPESIILESVLEGMAEYYSKNLAREVRKGLNENALVAKHNGGIPPLGYNV
                     TPTGGYEINPPEAEAVRIIFDMYSKGYGYSLICEELNRRGLKTKLGRAFGKGSIADIL
                     RNEKYIGRYVWNKRLSKKSGNRQYKPDDQITRIDGALPAIISKETWSKVQDILTFRKR
                     KPRHNQTYYYLLTGKMFCSECGSAFVGSGYEYGRGKKKNYQYSCNGRLRRKCECTNKP
                     IRCEKIEQYVLDTIGEVLSEDTIQSLADKMMDMLNEKAVSSCDTEKSLETKLEDVKRK
                     ITKTWALYYDGLLPKEDIVQQIGALTDQQKDVETKIVNASIFAEQPKFQRENVVAYLH
                     KCKTNLNSNDPDVLRSLVETFVDRIDISPENIKITIRVSPKAGMDSDKVGGDDGSRTR
                     VRR"
     gene            complement(1767..2141)
                     /locus_tag="VHR42_04415"
     CDS             complement(1767..2141)
                     /locus_tag="VHR42_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026453.1"
                     /translation="MKTTKLVSSIVSIVLAAAGICYELISHAKNFSGISCWLVAIAMI
                     VAGIIGIVMLSSKNWTVVATVIYAAAGIFGIIKSFKYSGLLIGSIVSLVIAVIFLISI
                     FKQDYSVAEKVHAPVSAPAPKQ"
BASE COUNT          524 a          548 c          525 g          658 t
ORIGIN      
        1 tgattcaaag gctaccaaag cgtgaagcct gcctttgggc gttgcgctga gggaatttta
       61 aatcataggc tacgctcgta gaaaaccttg gcaaattacc ttcggacacg ggttcgactc
      121 ccgtcatctc caccaacctt atcactatcc ataccagctt tcgggctgac gcggatagtg
      181 atttttatat tttcaggaga aatgtcgatt ctgtccacga aagtttcgac aagactgcgg
      241 agaacatccg ggtcgttgct gttcagattc gtcttacact tgtggagata ggcgacaacg
      301 ttttcccgct gaaatttcgg ttgttcggcg aaaatgctcg catttacgat tttggtctcg
      361 acgtctttct gctgatctgt aagagctccg atctgctgta cgatatcctc ttttggcagc
      421 aagccgtcat agtacagcgc ccacgttttc gtgattttac gttttacgtc ttccagcttg
      481 gtttcaaggc ttttctcggt atcgcaggac gaaaccgctt tttcattcag catgtccatc
      541 attttgtcgg ccagactctg gatcgtatcc tccgacagaa cttcaccgat ggtatcaagg
      601 acatactgct cgattttctc acagcggatc ggtttattag tgcactcgca tttgcggcgc
      661 aacctgccgt tgcaggaata ctgatagttc tttttcttcc cacggccgta ttcgtaacca
      721 ctgccgacaa aggccgaacc acattcagaa cagaacattt tcccggtgag gagatagtaa
      781 taggtctgat tatggcgcgg tttgcgcttc ctgaaagtca gaatgtcctg cactttactc
      841 cacgtctcct tcgaaatgat ggcgggcaga gccccgtcga ttcgcgtgat ctggtcgtct
      901 ggcttgtact gccggttgcc ggatttttta gagaggcgtt tattccagac atagcgtccg
      961 atgtattttt cattacggag gatatctgca atgctgccct ttccgaatgc cctgccgagc
     1021 tttgtcttca gaccacgccg gttcaactct tcgcagatca ggctataccc atagcctttt
     1081 gagtacatat cgaaaataat tctgacggct tctgcttcag gcggattgat ttcgtaaccg
     1141 ccggtgggag ttacattata tccaagcgga ggaataccgc cattgtgttt ggcaacaagc
     1201 gcgttttcat tgaggccctt tcgtacttcc cgcgcgaggt tctttgaata atactcggcc
     1261 atgccttcga gaacgctttc gaggatgatg ctttcggggc tgttgtccag cggttccagc
     1321 acggagacca ctttgacgcc acacttttcg agtgacttcc gatagaacgc gctatcatac
     1381 cgattacggg cgaagcggtc tagtttgtga acgatgcata gctgaaatcc gccgcctttt
     1441 gcatcctcta tcatttgaag aaaaccttcc cgatgatcgg tagtcgcgga aacggcttcg
     1501 tctttataaa catgggccag taaaatctga tttttacggc aatattccgt gatggcgcgg
     1561 acctgagcgt caatactttc ctgccgttga ttatcagaac tgtatcgggc gtatgcaacc
     1621 gcaatctgca tttttataac aacctttctt gttttagaaa ggctgacgtg atacaataaa
     1681 actgcgagtt atatcgtact gtcagccttg ctgatggtgt gccgtctcat ccggttgcag
     1741 ccgggtgggg cggatttttt atttaattac tgtttaggtg ctggagcgga aacaggtgcg
     1801 tgaacttttt ccgcaacact atagtcctgc ttgaaaattg aaatgagaaa gatgactgcg
     1861 atgaccaagc tgacgatact cccgattaag agccctgaat acttgaagct ttttataatt
     1921 ccgaaaatcc cggcagccgc atagataacc gttgccacaa ctgtccagtt ctttgatgaa
     1981 agcattacta tgccaataat tccagcgaca atcattgcaa tggctacgag ccagcaagaa
     2041 ataccgctga aattcttcgc atgactgatc agctcgtagc agattcccgc agctgctaag
     2101 acgattgata caatgctgga aacgagtttc gtcgttttca tatgtaatct ccccttttct
     2161 cttgacaaat gaagccaaga aattataatg tgctttggag attccttccg agaatttccg
     2221 gcttgtgctg ctcttcctta tcgcgagtag ggggg
//
LOCUS       DATALY010000151         6816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_638418,
            whole genome shotgun sequence.
ACCESSION   DATALY010000151 DATALY010000000
VERSION     DATALY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638418"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHR42_04420"
     CDS             <1..208
                     /locus_tag="VHR42_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HEX3026454.1"
                     /translation="MDETHTVLAVGAGGVSKLREPCGGRIERIFNFKYPYEYISRFQE
                     IVDRKSQVIKFYDEYNSQSKNQDQ"
     gene            168..1142
                     /locus_tag="VHR42_04425"
     CDS             168..1142
                     /locus_tag="VHR42_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HEX3026455.1"
                     /translation="MMNTILRAKIRISEVNKAASDNPSAFFSVCNGKYEQKVRSVAEK
                     IKERIAQTHIIMLSGPSASGKTTTSMKIKEELAKLGITAITISMDDFFKGKEKVPAQP
                     DGKKDFESISALDLDLLKSCLAGLIYKGRAELPVFNFKLGERDKKIRAVELSGGNVAI
                     VEGLHALDTVVTEDFPPENVFKLYVSVSSDFVGQSGDTILNARQMRLIRRTIRDFRFR
                     GSSPENTLDMWDDVCEGEDRYIRPFKKYADMTINSAFSCEPCLFRSQAEELFSKVPEG
                     SIHYDRAAELIRALSEFRKIPMEMVPSDSMLREFIGGSIYFNASAGKK"
     gene            1269..1625
                     /locus_tag="VHR42_04430"
     CDS             1269..1625
                     /locus_tag="VHR42_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX3026456.1"
                     /translation="MGVFEDVVIKAKTAADYVGNKTGEIVELSKMKVAAAELEGKIER
                     EYCELGREVYEAAKANTDVTEQIGLKTTAIDALKGELAGIYVKLDDLKGHKKCAACGF
                     VNIDEANYCIKCGEKL"
     gene            1727..2554
                     /locus_tag="VHR42_04435"
     CDS             1727..2554
                     /locus_tag="VHR42_04435"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX3026457.1"
                     /translation="MKMEDHRVSAEFILEQIGQKPVTAVVLGSGLGNLAEDMESACRI
                     DYRAIPHFPVSTAPGHKGELIFGCYHGADILIFSGRFHYYEGWSVEQSAYYVRVLKMM
                     GVRNLVLTNAAGCINEKFRPGDLMMVSDHINLTGLSPARGENCEEFGPRFFDMTDAYS
                     KVLRRAAAEYARLCGVPLREGVYAYMTGPQYETPAEIRALRVLGADAVGMSTVPEVIE
                     AAHCGLRTLCISCLTNYAAGISGRPLSGQEVVEAASQSAGGFSRLLGSLLPAVGKIE"
     gene            2753..3589
                     /gene="mazG"
                     /locus_tag="VHR42_04440"
     CDS             2753..3589
                     /gene="mazG"
                     /locus_tag="VHR42_04440"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX3026458.1"
                     /translation="MSVNFKAKDQYRFDDLLQIMEILRGENGCPWDREQDHQSIRVNF
                     IEETYEVIEAIDKQDTELLKEELGDVLLQIVFHSQMEKEEGSFDISGVIDGICKKLIV
                     RHPHVFGDVRADTSDEVLKNWDEIKKQQKKQTTQTEVMKTIPRFQPALMRSSKIQQKA
                     GKAGFDWPDVNGALEKTAEELEELKEAIRSGDHEHCRDELGDLLFSVVNVSRFIHVEP
                     EEALTLCSDRFIRRFSLVEKMALETGNNMKDLTLGQLDELWEQAKRQINAEEQTASGI
                     SN"
     gene            3648..3923
                     /locus_tag="VHR42_04445"
     CDS             3648..3923
                     /locus_tag="VHR42_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX3026459.1"
                     /translation="MTKTELITKVAEKGGFSKKDADKATNAVIDAIVEALASKDKVQL
                     VGFGTFEVHHRPARKGRNPRTKEEISIPESNYPVFKAGKSFKDAVSK"
     gene            3988..4236
                     /locus_tag="VHR42_04450"
     CDS             3988..4236
                     /locus_tag="VHR42_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX3026460.1"
                     /translation="MRLDKYLKVSRLIKRRTVANEACDAQRVKVNGKPARASYEVKPG
                     DVLELTLGGHTLRVEVLEVAQTVRKEGASDLYRVLEQN"
     gene            4393..4683
                     /gene="yabP"
                     /locus_tag="VHR42_04455"
     CDS             4393..4683
                     /gene="yabP"
                     /locus_tag="VHR42_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HEX3026461.1"
                     /translation="MAVLEEKKPMKKVHNVIIEDRKLLTVSGVSDVDSFDEGAVVLFT
                     EMGELEIRGANLHMNKLNVETGEVSVEGDIQALSYQDEGPRGSGGFFGRIFR"
     gene            4695..5267
                     /gene="yabQ"
                     /locus_tag="VHR42_04460"
     CDS             4695..5267
                     /gene="yabQ"
                     /locus_tag="VHR42_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HEX3026462.1"
                     /translation="MGISVTGQAIDFFLALLIGAALGAVYDVFRIMRLARSFGRVAVF
                     IQDAVYFLICAVTTFLFLLVDNSGVVRAFLILGVLLGAVVYSLTLGAAVIKAAKRMIV
                     PAKKIVRKAASDIAVPIRHVFGSAKEKIRISGGKVNIFTKKEFKVFKIRLQHHKRMLY
                     NQIRPTEKRMTVKQKKDRKRRRRKKSEKTS"
     gene            5251..5529
                     /locus_tag="VHR42_04465"
     CDS             5251..5529
                     /locus_tag="VHR42_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX3026463.1"
                     /translation="MKKHRKSLVLRIAVGVFIAYLAVTLVGQQIQIRKKQNELVSLSK
                     QYTAQKAKNDEIQRLLSGDKNEYIKSIARDKLNYVNPNERIYIDVSGN"
     gene            5564..5980
                     /locus_tag="VHR42_04470"
     CDS             5564..5980
                     /locus_tag="VHR42_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX3026464.1"
                     /translation="MQLEVGSILEGKITGITKFGAFVELPGGRTGMVHISEVAPTFVK
                     EIRDYVKENQVVKVKILTISEDGKVSLSMKRVDMPPAGKRPYRSRPDNYEWSPKRNDN
                     LSFEEMMNKFKQSSDEKLSDLKKYIDPKRGNRVSSR"
     gene            6246..>6816
                     /locus_tag="VHR42_04475"
     CDS             6246..>6816
                     /locus_tag="VHR42_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3026465.1"
                     /translation="MEDLEIRNGWIKIEGGKIASLGEMESPPSFDGEEVIDAKGGTVL
                     PGLIDAHTHLGMWEDSLGFEGDDGNEETDPSTPQLGAIDAINPLDRCFEDALSAGVTT
                     VVTGPGSANPIGGRMVAMKTYGRRIDDMILKNPVAVKFALGENPKTVYHGKDAAPQTR
                     MATAAIIREQLIKARKYMRDMEESCQDEDA"
BASE COUNT         1917 a         1428 c         1868 g         1603 t
ORIGIN      
        1 aatggatgag acccacaccg tgcttgcggt aggtgcgggc ggcgtttcca agctgcggga
       61 gccttgcggc ggcagaatcg aacgcatttt taatttcaaa tacccttacg aatatatatc
      121 ccgctttcaa gaaattgtcg acaggaagag tcaggtgatc aaattctatg atgaatacaa
      181 ttctcagagc aaaaatcagg atcagtgaag tcaataaagc cgcttcagac aatccctccg
      241 cctttttcag cgtatgcaat gggaaatatg aacaaaaagt gcgttcggtt gctgaaaaga
      301 tcaaagaacg aatcgcccag acgcatatta tcatgctgtc gggtccttct gcgtcgggta
      361 aaacgacgac ttccatgaag atcaaggaag aactggcgaa actgggaatt accgcgatta
      421 ccatttctat ggatgatttt tttaagggca aggaaaaagt ccccgcacag ccggatggaa
      481 aaaaggattt cgagtcgatc tcggctcttg atcttgattt gctcaagagc tgtctggccg
      541 gcctgattta caaaggccgg gccgagctgc cggtattcaa ttttaaactt ggcgaacggg
      601 ataaaaaaat ccgcgcggtt gagctttccg ggggcaatgt tgccatcgtt gaaggtctgc
      661 acgcgctgga tacggttgta accgaagatt ttcctccgga aaacgttttt aaactatatg
      721 tcagcgtcag ctccgacttt gtgggccaaa gcggcgatac cattctgaac gcccgccaga
      781 tgaggctgat ccggagaacc atccgggatt tccggttccg gggcagcagc ccggagaata
      841 cgctggacat gtgggatgat gtctgtgagg gggaggaccg ctatatccgc ccgtttaaaa
      901 aatatgccga tatgaccatt aattccgctt tcagctgcga gccttgcctt tttcgctcac
      961 aggccgaaga actgttttca aaggtcccgg agggcagcat tcattatgac agggcggcag
     1021 agctgatccg ggcgctgtct gaattcagaa agataccgat ggagatggtt ccgtctgaca
     1081 gtatgctccg tgaatttatc ggcggcagca tctattttaa cgcgagcgcg gggaaaaaat
     1141 aattgttgtg atccggctgc gcgccgtatt gtgaatttgc attttgaatg atataatgaa
     1201 cctgagaaga ttaggcaata ccggaatatt tgacctttta aacttatttt agacaaggag
     1261 tgacacacat gggtgttttt gaggatgtcg tcataaaagc gaaaaccgct gcggattacg
     1321 taggcaacaa gaccggtgaa attgtggaac tctccaagat gaaggtcgct gccgcggaac
     1381 tggaaggtaa aatcgaaaga gaatactgcg agcttggccg ggaggtctat gaagctgcaa
     1441 aggcaaatac cgacgttacc gaacagatcg ggctgaaaac caccgcaatc gacgctctga
     1501 aaggcgagct tgcgggaatc tatgtgaagc tggatgatct caagggccat aaaaaatgtg
     1561 ctgcgtgcgg ttttgtcaac attgacgagg caaattactg cattaaatgc ggagagaaac
     1621 tttagattta atcggacaga aaaaagggga aaaagccttt acggcgtctc cccctttttt
     1681 tcatgccgca aatgcgcggc ggattgagaa aagacggtga atcggaatga aaatggaaga
     1741 tcatcgggtc agcgcggagt tcattttgga gcagatcggg caaaagcctg tcactgccgt
     1801 ggttttgggc tcgggtctcg gaaatctcgc ggaagatatg gagtctgcct gccggattga
     1861 ctatcgcgcg attccgcatt ttccggtttc caccgctccg ggtcataagg gagagctgat
     1921 tttcggctgt tatcacggcg cggatatcct gatcttttcc ggccggtttc attattacga
     1981 aggctggagc gtcgagcagt cggcttatta tgtgcgtgtg ctcaaaatga tgggagtccg
     2041 taaccttgtc ctgaccaacg ccgcgggatg catcaacgaa aagttccggc cgggcgattt
     2101 gatgatggtc agcgaccata tcaaccttac ggggctaagc ccggcaagag gggaaaactg
     2161 tgaggaattc ggccccaggt ttttcgatat gaccgacgcg tacagcaagg tcctgagacg
     2221 tgcggctgcg gaatacgccc ggctttgcgg cgttcccctg cgggaaggcg tttatgccta
     2281 tatgacgggg ccgcagtatg aaacacctgc ggaaatccgc gccctcaggg ttctgggagc
     2341 ggacgccgtg ggaatgtcaa ccgttccgga agttatcgag gccgcccact gcgggcttcg
     2401 aaccctctgc atatcctgcc tgaccaatta cgcggcagga atatccggcc gtccgttgtc
     2461 ggggcaggag gtagttgagg ccgcgtcgca gtcagccgga ggcttctcgc ggctgctcgg
     2521 gtcattgctt cccgcagtcg gcaaaattga atgatttttt ggaaatggtg aataataagc
     2581 gtaatcagtc aggatgtttt catggaaatt tcgctgtttc cagaggaaaa cttgccttga
     2641 aaaagaaaaa gtggcgattt tatacgaaaa attcttgaaa aacttaaata gatagggtaa
     2701 tatattactg tttagtaaat atttcatatt tttttgggaa accggagaaa aaatgagcgt
     2761 gaattttaag gcaaaagatc agtatcgttt tgacgacctg ttgcagatca tggagattct
     2821 gcgcggtgaa aatggctgcc cgtgggatcg ggagcaggat caccagtcga ttcgcgtcaa
     2881 ttttattgaa gaaacttatg aagtcattga ggcgatcgac aagcaggata ccgagttgct
     2941 caaagaagaa ctcggagatg tgctgcttca gattgtgttc cattctcaaa tggaaaagga
     3001 agagggctct tttgacattt ccggcgtaat cgacggaatc tgcaaaaagc tgatcgtccg
     3061 gcatccgcac gtatttggcg atgtcagagc ggataccagc gatgaggttc ttaaaaattg
     3121 ggatgaaatc aaaaaacagc aaaaaaaaca gaccacacag acggaagtca tgaaaacaat
     3181 tccgcgcttt cagccggctc tgatgcgaag cagcaagatc cagcaaaagg caggcaaggc
     3241 ggggtttgac tggcccgatg taaacggtgc gctcgagaag acggccgaag agctggaaga
     3301 gctcaaagag gctatccgca gcggggatca tgaacattgc cgggatgagc tgggcgacct
     3361 gcttttttcg gtagtgaatg tgtcccggtt tatccatgtt gagccggagg aagcgctgac
     3421 gctttgttcc gatcggttta tccgtcgttt ttcgctggtc gaaaagatgg cccttgaaac
     3481 cggaaacaac atgaaggatc tgacactcgg gcagctcgat gagttgtggg agcaggcgaa
     3541 acggcagatt aatgccgaag agcagaccgc ttccggaatt tctaattaag ctatttatga
     3601 atgtatcatt cataatagta tagaacccaa aaacggaggg atataaaatg actaaaacag
     3661 aactcataac aaaagttgcg gaaaaaggcg gattttccaa gaaagacgcc gacaaagcga
     3721 caaatgccgt catcgacgcc attgtagagg cgctggcatc caaagacaag gtccagctgg
     3781 taggcttcgg tacattcgaa gtacatcacc gcccggcgag aaaaggccgt aacccgagaa
     3841 ccaaagagga aatctccatt cccgagtcga actacccggt tttcaaagcc gggaaatcgt
     3901 tcaaagacgc tgtctccaag taatgcgagg gggaaccggg gcaacccggt tccctgattt
     3961 tatgttacga aaagtgaaga aaaaacaatg agattagata aatatttaaa agtttccaga
     4021 ctgatcaaac gaagaacagt ggccaacgag gcctgcgatg cccaaagggt aaaggtcaac
     4081 ggcaagcccg ccagagcctc ttatgaggtc aagcccggcg atgtgctgga acttaccctt
     4141 gggggccata cgctccgtgt cgaggtgctt gaagtagccc agacggtgcg taaagaagga
     4201 gcgtcggatc tttaccgggt tcttgagcag aactgagcgc ttatggagca tcccggagaa
     4261 cgggcaaagc cggtttcgga aattaatccg aagccggctc tttttgtctc ttgtgcggta
     4321 atatttttga ataagagcac atagattgtt atgggcttgg cttaggaata ttatattgcg
     4381 gagggattaa aaatggctgt gctggaagaa aaaaagccga tgaaaaaggt tcataatgtg
     4441 attatcgaag accgaaagct gctgacggtg tcgggtgtat ccgatgtcga cagcttcgac
     4501 gagggagccg tagtgctgtt taccgagatg ggtgaacttg aaatccgggg agccaatctc
     4561 cacatgaaca agctgaatgt ggaaaccggg gaagtaagcg ttgaagggga tattcaggcg
     4621 ctgtcctacc aggacgaggg gccgcgcggt tccggcggat ttttcggaag gatattcaga
     4681 taggatgatg gcttttgggt atctcggtta cgggtcaggc aattgatttt tttcttgccc
     4741 tgctgattgg ggccgctctg ggagcggttt acgatgtgtt tcggattatg cgtctggccc
     4801 gctcttttgg ccgcgtggcc gtctttattc aggacgcagt ctattttctg atctgtgcgg
     4861 tgaccacctt cctgtttttg ctggtagata attcaggtgt tgtccgggct tttttgattt
     4921 taggtgtgtt gctgggggcc gtcgtctact cccttacact gggggccgcc gtcatcaaag
     4981 cggcgaagcg aatgatcgtt cccgcaaaaa aaattgtccg caaggccgct tcggatatag
     5041 cggttccaat ccgccatgtt tttggttcgg caaaagaaaa aatccgaata agtggtggaa
     5101 aagttaatat tttcacaaaa aaagaattta aagtttttaa aatacgcttg caacaccata
     5161 aacgaatgtt gtataatcaa atacgaccaa cggaaaaacg tatgactgta aagcaaaaaa
     5221 aggacagaaa acgtcggagg cggaagaaaa gtgaaaaaac atcgtaaaag cctcgttttg
     5281 agaattgctg tcggtgtgtt tattgcctat cttgccgtga cgcttgtcgg ccagcagatc
     5341 cagatccgaa aaaaacaaaa tgagttggtc agtctgagta agcaatatac ggctcaaaaa
     5401 gcaaagaacg atgaaattca acggctccta tccggcgata aaaatgagta tatcaaaagc
     5461 atcgccaggg acaagctgaa ttatgtgaat ccgaatgaac ggatttatat tgatgtttcc
     5521 ggcaactagt ttctgaaata tttaaggagg aaaactattt tttatgcagc ttgaggttgg
     5581 gagtatactg gaaggtaaaa ttacgggtat aacgaaattc ggagcatttg tggaacttcc
     5641 cggcggcagg accggtatgg ttcacatatc cgaagtcgcg ccgacgttcg taaaggaaat
     5701 cagagactat gtcaaggaaa atcaggttgt taaagtaaaa atcctgacca tttccgaaga
     5761 cgggaaagtc agtctgtcga tgaagcgggt tgatatgccg ccggcgggca aaagaccgta
     5821 tcggtcaagg cctgataact atgaatggtc tcccaaaaga aacgacaatc tgtcttttga
     5881 agaaatgatg aataaattca aacagtcaag tgatgaaaag ctttctgatt tgaagaaata
     5941 tatcgacccc aaaagaggca accgggtttc ttcccgatag cccgtgaacg gcgaaattct
     6001 cgataaagaa tgaaattttt gaacggagtg tttattacac tccgtttttg cattataatt
     6061 agtgatgata tttgggcaaa aaagtgccgc ctttcagaaa aaaccaaaag gcggttacaa
     6121 aaaaagtttt gggaagtgaa agggttatga agggagttgc aagataataa taccatattt
     6181 atcaaaatat gtaaatagat aaaggagaga cggtcttggt cattatcaac gcggttattg
     6241 atacgatgga agatttggaa atccgtaatg gatggataaa aatcgaggga gggaaaatcg
     6301 catcgctggg cgaaatggaa tctccgcctt cttttgacgg agaagaagtc attgacgcaa
     6361 aaggcggaac ggtgcttccc ggtctgatcg acgcgcacac ccacctgggc atgtgggagg
     6421 acagcctggg ttttgagggc gatgacggta acgaagaaac cgatccttcc actccacagc
     6481 ttggggcgat tgacgccatt aaccctctgg accgctgctt tgaggacgcg ctttcggcag
     6541 gcgttaccac agtagtaacc gggcccggca gcgcaaatcc catcggcggc cggatggtag
     6601 caatgaagac ctacggccgt agaattgacg atatgatttt aaaaaacccg gtggcggtta
     6661 agtttgctct tggcgagaat ccaaaaaccg tctatcacgg caaggacgcg gcgccgcaga
     6721 ccaggatggc gaccgccgcg attatacgcg agcagctgat caaagcgcgc aaatatatga
     6781 gagacatgga agagtcctgt caggacgagg acgccg
//
LOCUS       DATALY010000152         8185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_638486,
            whole genome shotgun sequence.
ACCESSION   DATALY010000152 DATALY010000000
VERSION     DATALY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638486"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VHR42_04480"
     CDS             <1..353
                     /locus_tag="VHR42_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026466.1"
                     /translation="LPTSVSASGVIRGGQAVCYLSTEDASKIKAGQTVAVQAVEQNGT
                     FNGKVSDVGMTPLSSSEVAAELGSDYLTSILATTKYSVRTVIAVPQTGIADGTVLNLK
                     IIVEEKRPFDFLGG"
     gene            364..1989
                     /locus_tag="VHR42_04485"
     CDS             364..1989
                     /locus_tag="VHR42_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX3026467.1"
                     /translation="MTKIVKVPVVIQMEALECGAACLAMILAYYGKWVPLEQMRSDCG
                     VSRDGSNARNILRAARAYGLDAAGYRLEMEDIKKVEFPVIIHWNFNHFVVLDGFRKGR
                     AILNDPGRGTVEVPMEEFEKAFTGIVLQFQKTERFIPGGKPRSVAAFARERLKGTLVP
                     FLFVIITGVLTAAVGVISPAFSRVFMDRILTGRNPNWLYPFLFAMASLLVFQIAVSLI
                     NTLYMLKIRGKLAIVANTKFFWHVVRLPMEFFSQRMAGDIAARQATNETIAETLISQL
                     APVLLNIGMLVFYLAVMLRYSLLLTCVGLTSTFLNILVARRISKKRINITRAQMRDGG
                     NLAAMTMNGFEMIETIKAAGAESGFFERWAGYQASVSNAGVNTAKLNQYLGSVPALLQ
                     QLSNIAVQVLGVMLIMKGSFTAGMLLAFQSFMGSFTGPVSTLIGTGQSVQQMRTSMER
                     IEDVMNYAPDVEYSEKPEVSDEEYEKLRGAFSMKDVTFGYSKLAPPADRAFQSGSETG
                     RPGGVCRNLRLRKIHARKTDIRPLQTVERSYRV"
     gene            1895..2512
                     /locus_tag="VHR42_04490"
     CDS             1895..2512
                     /locus_tag="VHR42_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3026468.1"
                     /translation="MAFVGTSGCGKSTLAKLISGLYKPWSGRIEFDGKEQGEISREIF
                     TGSLAVVDQDIILFEDSIANNIKMWDKSIEDFEMILAARDASLHTEIMLRDGGYNSIV
                     MEGGRNFSGGQRQRIEIARVLAQDPRIIVLDEATSALDAKTEYEVINAIKDRGITCII
                     VAHRLSTIRDCDEIIVMENGKVAERGRHDELYRSGGLYTQLIAME"
     gene            2524..4731
                     /locus_tag="VHR42_04495"
     CDS             2524..4731
                     /locus_tag="VHR42_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HEX3026469.1"
                     /translation="MGKGWFDEQIKYRIENDQESFENVFADLSSVVLGKSSVSATFDS
                     DRVKTKTAIEEILKYFKVKPIELPEEIEDKNDQLEYLLRPTGIMRRKVKLQGKWWRDA
                     IGPMLGQTLNGDTVALIPAGVSGYRFFDYETGQSIQLSGKTKGLIQEDAYCFYRPLPL
                     KKLEIADLAKYMITSLARADILSIVLASFAASLIGLFTPYVTKLIYANVIPSGETGLL
                     LPVAVMLLSVTISTMLINAAKSLLTARIQTKLSVYVESAAMSRTLFLPAEFFKQYNTG
                     ELSSRVSAIHQLCDSLINAFLSTGLTALFSFVYLAQIIGFAPALALPAMIIILVQLAF
                     TVLTSLAKLNLTRRQMKIAAKLNGLTFSIFSGIQKIKLAGAEKRIFAKWGQAYKEEAQ
                     LSYDPPALIKIQPIFAGIISMAGLVAIYYSAAVSNVSVADYMAFNSAYGMVSGAIMSL
                     AGVAMTFANIKPIMEMAEPILKAEPEIGENKKIVTRLSGQIEINNVSFRYNEEMPLVI
                     DNLSLKIRPGQYIAIVGKTGCGKSTLMRLLLGFECPQKGAVYYDGQDIAKLDLRSLRQ
                     NIGSVMQNGKLFTGNILSNITVSSPWLGLKEAWEATELAGIADDIRAMPMGMHTMISE
                     GSGGISGGQRQRLLIARAIVAKPRILIFDEATSALDNITQKHVSDALDSLNSTRIVIA
                     HRLSTIRHCDRIIVLDGGCIREDGNYEELIQKGGYFAELVERQRLGNEQPSKI"
     gene            4815..5204
                     /locus_tag="VHR42_04500"
     CDS             4815..5204
                     /locus_tag="VHR42_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HEX3026470.1"
                     /translation="MSKENIEKFQEELKKRPELQQKLKETETTEDFIALAKAEGFDFS
                     AEEFTEYARSGKLSDEQLGEVTGGGLYGFDNDLIVTAGYGCTYWTARRTLWLAAKGNC
                     GSCECYDQKGLFGGLCLNKNNSLHDGC"
     gene            5356..5634
                     /locus_tag="VHR42_04505"
     CDS             5356..5634
                     /locus_tag="VHR42_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026471.1"
                     /translation="MSKDDAEKNKLPDNELDRVAAGSGQLYDRNYGNCSCDKVKDDST
                     FYNYFYAIFNAYGYYHITTPVDCPDWSAIGTNPFHCCQNCKNLHGTGL"
     gene            5696..5959
                     /locus_tag="VHR42_04510"
     CDS             5696..5959
                     /locus_tag="VHR42_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026472.1"
                     /translation="MSKKNENRLTNIKESAETKILSDKELDKAAAGFGPYATYTLECR
                     TCHEIILTIDLNYSIDVTKFVELRRNAENKHTQETGHTQFGLI"
     gene            6018..6290
                     /locus_tag="VHR42_04515"
     CDS             6018..6290
                     /locus_tag="VHR42_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026473.1"
                     /translation="MFDLSKYDFSKGSDPFKEALLKKCLSRLSVASADRISDEELEYV
                     AAAGTGNNETGCPFPGRPCGSCTHYLPGQPEMCGLGCRKPEDGSDG"
     gene            6283..6825
                     /locus_tag="VHR42_04520"
     CDS             6283..6825
                     /locus_tag="VHR42_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3026474.1"
                     /translation="MVDLQDSCGFEQMYEAYFRRIYNYVFCRLLHKEQTEDVVSRIFM
                     KVLEKRQSYDERKASFNTWIFTIARNTLNDFYRLRKAEVSIDDDRFAEPAINFEEQSR
                     LIADEELRELYMALTDLDEKTRMVITLKYFGEFTNREISVQTGINESTVSTLCGRGIA
                     KLQKTMESMQKTGRGCGSQT"
     gene            6847..7659
                     /locus_tag="VHR42_04525"
     CDS             6847..7659
                     /locus_tag="VHR42_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HEX3026475.1"
                     /translation="MVSTGSILNLLIELIKNMSVLIVLAYILTRTKIYAEIIQSKEAA
                     SLKQKVCLTLICGIFAIYGTLSGIKIGGAIANVRDLGPAIAGLIGGPFAGLAAGLIGG
                     IHRYFVGGFTYLSSSASTVVAGLAGGMIYRYRKGKFISVAGGAAFMALMEIFHMGLLL
                     AIERPFDQAMLLVENIGLPMIFTDALGMSIFAFIVRNFLNEKETQRTKELIESELSIA
                     RDIQMSIVPKIFPASPELPESILPSTVKTKTSFFFACRTSITGLLIICCQEI"
     gene            complement(7671..7967)
                     /locus_tag="VHR42_04530"
     CDS             complement(7671..7967)
                     /locus_tag="VHR42_04530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026476.1"
                     /translation="MSLDEILTNTVGYGFKDSEEHEIIVKFVLYNKTLTIEIRDDGIP
                     FNPLKQPEPDLSQNIEERPIGGLGIHLVRKMMDAVDYRREEGCNILTIKKHIAT"
     gene            7939..>8185
                     /locus_tag="VHR42_04535"
     CDS             7939..>8185
                     /locus_tag="VHR42_04535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX3026477.1"
                     /translation="MFVSISSSDSLLVYTDGVTEAMNSAGELFGEQRLKYILHDSGGG
                     TAQEEVRHVLAKTREFVNGAVQSDDITILALEYLRRVQ"
BASE COUNT         2121 a         1920 c         2153 g         1991 t
ORIGIN      
        1 gtctgccgac cagcgtcagc gcttccggcg ttattcgcgg tgggcaggcg gtctgctatc
       61 tgagtacgga agatgcttcc aagataaaag ccggacagac tgtcgcggtg caggcggtag
      121 agcagaacgg cactttcaac ggaaaagtgt ccgatgtcgg gatgacgccg ctttcgtcgt
      181 cggaggtagc cgccgagctt ggaagcgatt atctgaccag tattttggca actactaaat
      241 attctgtcag gacagtcatt gctgtgccgc aaacaggcat tgccgacgga accgttttaa
      301 atttgaaaat tatcgtggaa gaaaaaagac cgttcgattt tctgggtggt tgaggtgaag
      361 catatgacta aaatcgtaaa ggttcccgtc gtcatacaga tggaggcttt ggaatgcggc
      421 gccgcttgtc ttgccatgat acttgcctat tacggcaaat gggtgcccct cgagcagatg
      481 cgctcggact gcggcgtttc gcgtgatgga agcaatgcgc gaaacatcct gcgcgcagcc
      541 cgcgcctatg gtctggatgc ggcaggatac cgtcttgaaa tggaggacat caaaaaagtg
      601 gaatttccgg tgatcatcca ctggaatttc aaccatttcg tagtgcttga cggcttcaga
      661 aaaggccgtg ccatacttaa tgacccggga cgtggaaccg ttgaagtccc tatggaggaa
      721 tttgagaaag cttttaccgg catcgttctc cagtttcaaa agacagaacg ctttatcccc
      781 ggcggaaaac cgcgcagcgt ggcggctttt gcgcgggaac ggctgaaagg cacgttggtg
      841 cccttccttt ttgtgattat aaccggcgtt ctgaccgccg ccgtgggcgt gatttctccc
      901 gccttttccc gcgtgttcat ggaccggatt ctgaccgggc ggaacccaaa ctggctttat
      961 cccttccttt tcgcaatggc atccttgctg gtatttcaga tcgcggtttc gttgatcaat
     1021 acactgtaca tgctgaaaat ccggggaaaa ctcgccattg tggctaatac caaattcttt
     1081 tggcacgtag tccggctgcc aatggaattc ttttcacagc gcatggccgg agatatcgcc
     1141 gccaggcagg ccaccaatga gactatcgct gaaacactga tctcacagct ggcgccggtg
     1201 cttctgaata tcgggatgct ggtcttctat ctggcggtca tgctgaggta cagtctattg
     1261 ctgacctgcg tcgggttgac ctcaacgttt ctgaatattc tcgtcgcacg cagaatttcc
     1321 aaaaaacgta tcaacatcac ccgcgcccag atgcgtgacg gcggcaacct ggctgccatg
     1381 accatgaacg gttttgaaat gattgaaact attaaagcag ccggggccga gagcgggttt
     1441 ttcgagcgct gggccggcta tcaggcgtcc gtcagcaacg ccggggtcaa tacggctaag
     1501 ctcaatcaat atctggggtc cgtccctgct ctgttgcagc agctttccaa tatcgcagtg
     1561 caggtactgg gcgtcatgct gatcatgaaa ggcagcttta cagcgggcat gcttcttgcg
     1621 tttcaaagct tcatggggtc ctttaccggc cctgtcagca cattgatcgg aacggggcag
     1681 agcgtgcagc aaatgcgtac atctatggaa cgtatcgagg atgtcatgaa ttatgcgccg
     1741 gacgtggaat attccgaaaa gccggaggta agcgacgaag aatatgagaa gctgagaggc
     1801 gccttttcaa tgaaggacgt gaccttcggg tactcaaaac tcgccccccc cgctgatcga
     1861 gcatttcaat ctggaagtga aaccgggcga ccgggtggcg tttgtcggaa cctcaggctg
     1921 cggaaaatcc acgctcgcaa aactgatatc cggcctttac aaaccgtgga gcggtcgtat
     1981 cgagtttgac ggtaaagaac agggagaaat cagccgggag atttttaccg gctcgctggc
     2041 ggttgtcgat caggacatca tcctctttga agacagcatt gccaacaaca tcaagatgtg
     2101 ggacaaatcc atcgaggact ttgagatgat cctggcggcc cgcgacgcat ccctgcacac
     2161 ggaaattatg ctgcgtgacg gcgggtacaa cagtattgtc atggaaggcg gcaggaactt
     2221 ttcgggaggc cagagacaga gaattgaaat tgcccgtgtg ctggcgcagg acccgaggat
     2281 catcgtcctg gacgaagcga cctcggcttt agatgctaaa acggaatatg aagtgataaa
     2341 cgccatcaaa gaccgaggaa tcacctgtat tatcgtggcc caccggcttt ccaccataag
     2401 ggactgcgac gaaatcattg taatggagaa cggcaaggtt gcggaacgag gcaggcacga
     2461 cgagctgtat agatccgggg ggctttatac gcagctgatc gccatggaat aggagagaca
     2521 tcaatgggca agggctggtt tgacgaacag ataaaataca gaatcgaaaa cgatcaggaa
     2581 agctttgaaa atgttttcgc cgatttgtct tcggttgtgc tgggcaagtc ctctgtttcg
     2641 gccacgttcg attccgaccg cgtcaaaacc aaaacggcga tcgaggaaat cttaaaatat
     2701 ttcaaggtaa aacctataga gcttccggaa gaaattgagg ataaaaacga ccagctggaa
     2761 taccttctgc ggccgacggg gattatgcgg cgaaaggtaa agctacaggg gaaatggtgg
     2821 cgcgacgcga tagggccgat gcttgggcag accttaaacg gcgatacggt ggctctgatc
     2881 cccgcagggg tttccggata ccggtttttc gattatgaaa cgggacaaag cattcagctc
     2941 tccggtaaaa caaaagggct tattcaggaa gacgcctatt gcttctatcg cccgctgccg
     3001 ctgaaaaagc tcgaaatagc ggacctcgcc aaatatatga taacgagcct tgcaagggcc
     3061 gatattttaa gcattgtcct tgcaagcttc gcggcaagcc tgatcggatt gtttacacct
     3121 tacgtcacca aacttatata cgcgaacgtg ataccatccg gcgaaacggg attgttgctt
     3181 ccggttgccg ttatgctgct gagtgtaacg atttccacca tgctgatcaa cgcggcgaaa
     3241 agtctgctga cggcgcggat tcaaaccaaa ctgtccgtct atgtggaatc cgccgcaatg
     3301 agtcggacgc ttttcctgcc ggcggaattt ttcaagcaat ataataccgg cgagctcagc
     3361 tcgcgtgttt cggctatcca tcagctgtgc gacagcctga tcaacgcatt tctgtccact
     3421 gggctcaccg cgcttttttc gtttgtctat ctggcgcaga tcatcggttt tgcaccggct
     3481 ctggctctgc ccgctatgat cattatcctc gtacagcttg cgtttaccgt tttaacaagc
     3541 ctcgcgaaac tgaacctgac ccgcagacag atgaaaatag ccgcaaagct gaacgggctt
     3601 actttttcga tcttttcagg gattcagaaa ataaagctgg caggcgctga aaagcgcata
     3661 tttgccaaat ggggacaggc ctacaaagag gaagcacagc tttcatacga tcctccggca
     3721 ctgatcaaga ttcagccgat attcgccggc attatatcca tggccggtct ggttgccatc
     3781 tattacagcg ccgcagtttc caatgtttcc gtcgcggatt atatggcttt caacagcgct
     3841 tacggtatgg ttagcggcgc cattatgtcg cttgcaggcg tagccatgac ttttgccaat
     3901 atcaagccga ttatggagat ggcggaacct attttaaagg cggagccgga gatcggcgaa
     3961 aacaagaaaa ttgtaaccag actttcgggg caaattgaaa taaacaatgt ttccttccgt
     4021 tataacgagg aaatgccgct tgttattgat aatctgagct tgaaaatacg gccgggccag
     4081 tatattgcca tcgtcggcaa aacaggctgc ggcaaatcga ccctcatgcg gctgcttctg
     4141 ggctttgagt gcccgcagaa gggcgccgtt tattacgacg ggcaggatat cgcaaaatta
     4201 gacctgcgtt ccctgcgcca aaacatcggc tcggtcatgc agaacggcaa actgttcacc
     4261 ggcaatattt tatccaacat taccgtttcg tccccatggc ttggattaaa ggaagcctgg
     4321 gaagcgacgg agcttgcggg catcgccgac gacatcaggg cgatgccgat gggcatgcat
     4381 actatgattt ccgaaggaag cggcggcatt tccggcgggc agcgccagcg tctgctgatt
     4441 gcgagggcaa tcgtcgcaaa accgaggatc ctgatttttg acgaggcgac ctccgccctt
     4501 gacaatataa cgcagaagca tgtttccgac gcgctggatt cgcttaacag cacccgcatc
     4561 gtcatcgcgc accgcctttc caccatccgc cactgtgacc gcatcattgt gctggacggc
     4621 gggtgtatcc gggaagacgg aaattacgaa gagctgattc aaaaaggcgg gtatttcgcg
     4681 gagcttgtgg aacggcagcg gctcgggaat gagcagccat ccaaaatatg atgaaatttt
     4741 acaagttttg agctgctttg aaaagtcatt cgtatatata tgcaggacat aaaaaacgaa
     4801 aggagagatt gcttatgtca aaagaaaaca tcgaaaaatt tcaggaggag cttaaaaaaa
     4861 ggccggagct tcagcaaaag ctgaaggaaa ccgaaaccac ggaagacttt atcgcacttg
     4921 ccaaagcgga aggcttcgat ttcagcgcgg aggaattcac agaatacgcg cggagcggca
     4981 aactttcaga cgaacagctt ggcgaggtga cggggggagg actgtacggc tttgataacg
     5041 atctgatagt aacagccgga tacggatgta cgtactggac ggcaaggcgc accctttggc
     5101 ttgctgcgaa aggcaattgc ggctcttgcg aatgttatga tcagaaaggc cttttcggtg
     5161 gtttatgtct gaacaagaat aatagtttac atgatggttg ctgactttac tgcatgtttt
     5221 attgccaaag taattatagc aggtgaagta tactatgcgt aatattgcgg tgttgcatca
     5281 ggatataaga taggttgacg ttgtttatct acaagaacat atttgaagta aaaataaggt
     5341 tataggaggt atattatgtc aaaggacgat gctgaaaaaa acaagctgcc ggataatgaa
     5401 ttagaccgtg tggcagctgg aagcggacaa ctttacgacc gtaattatgg aaattgctca
     5461 tgtgataaag taaaagatga ttctacattt tataattact tttatgctat ttttaatgct
     5521 tatggttatt atcatataac tactcctgtt gactgtccgg actggagtgc cattggcact
     5581 aaccctttcc actgttgtca aaactgcaaa aatcttcatg gtacaggatt gtgatacact
     5641 tacgaagtta tgtcgaagga aaagctttat tctttattta taggaggcta ataccgtgag
     5701 caagaaaaat gagaatcgct taacaaacat aaaagaatct gccgagacta aaatcctttc
     5761 ggataaagaa ttggacaagg cagcagcggg gtttgggcct tatgctacct acactttaga
     5821 atgtcggacg tgtcatgaaa tcattctgac gattgatcta aattactcaa tagatgtaac
     5881 taaatttgtt gaattacgga gaaatgctga aaacaagcac acccaggaaa ccgggcacac
     5941 tcaattcgga ctgatataac cggcttgatc tatacactta tatattcatg ggcttatagc
     6001 cacaggagaa cattaccatg tttgatctga gtaaatacga tttttcaaaa ggttccgatc
     6061 cctttaagga ggccctgttg aagaaatgcc tgtcacgcct ttcagtggct tccgcggatc
     6121 ggatcagcga cgaagagttg gaatatgtgg ccgccgcggg aacgggaaac aatgaaacag
     6181 gctgcccatt ccccggacgt ccctgtggga gctgcacgca ttatctgccg ggtcagccgg
     6241 aaatgtgcgg cctcggctgc cggaagccgg aggatggatc cgatggttga tcttcaggac
     6301 tcctgcggct ttgaacagat gtacgaagct tattttcgcc ggatatacaa ctatgtattc
     6361 tgcaggcttt tacataaaga acagaccgaa gacgtggtct cccggatatt catgaaggtc
     6421 cttgaaaagc ggcagagcta cgatgagcgg aaggcgtctt tcaatacatg gatttttaca
     6481 atcgcccgaa atactctgaa cgatttttac cggctgagaa aggccgaagt ctcgattgac
     6541 gacgatcgtt tcgcagaacc cgcgatcaat tttgaggagc aatcccgcct gatcgccgat
     6601 gaagaactgc gggagcttta catggcgctg accgatctgg atgaaaaaac gcggatggtg
     6661 attaccttaa aatacttcgg cgaatttaca aaccgagaga tttcggttca gacaggcatc
     6721 aacgagagca cggtttcgac cctgtgcgga cgcggtatcg ccaagctgca aaagactatg
     6781 gaatctatgc agaaaaccgg gcggggttgc ggatcgcaga cttgatggaa aggggacgag
     6841 aacaggatgg tttccaccgg cagtattcta aacttactga tcgaactgat aaaaaatatg
     6901 tccgtgctga tcgtgcttgc ctatatcctg acaagaacaa aaatttatgc agaaattatt
     6961 cagtcaaaag aggcggcgag ccttaaacaa aaggtctgcc ttacgctgat ctgcggaatc
     7021 tttgcgatat acggcacgct cagcggcatt aagatcggag gggctatcgc caacgtccgt
     7081 gatttggggc cggcaatcgc aggtctgatc ggcggaccct ttgcgggact tgccgccggg
     7141 ctgatcggcg ggatccaccg ttatttcgtc ggcggattca cctatctttc gagctccgcc
     7201 tccacggtgg ttgccgggct ggccggcgga atgatctaca gatatagaaa aggtaaattt
     7261 atcagcgtcg ccggaggcgc ggcctttatg gcgctgatgg aaatctttca tatgggcttg
     7321 cttctcgcga tcgaaaggcc gttcgaccag gcgatgcttt tagtggaaaa tatcggactg
     7381 cctatgatct tcaccgacgc tttaggaatg tccatttttg cgtttattgt ccgcaatttc
     7441 ttgaacgaaa aggagaccca gcgtaccaag gaactgatcg aaagcgagct gagcattgcc
     7501 cgggatattc aaatgagcat cgttccgaaa atattccccg cttctcccga gcttccggaa
     7561 tcgatccttc cttcgaccgt aaaaactaaa acatcatttt tttttgcctg caggacatcc
     7621 ataacgggcc tccttatcat atgctgtcaa gaaatatgaa cgccgtattt ctacgtcgct
     7681 atatgctttt tgatcgtcaa tatattgcag ccttcctctc gccggtaatc gacggcatcc
     7741 atcatcttcc ttacgagatg gataccgagt cctcctatcg gccgctcttc aatattctga
     7801 ctcagatccg gctccggctg cttcaaaggg ttaaacggga ttccgtcgtc acggatttcg
     7861 atcgtcagcg ttttattgta aaggacaaat ttaacgatta tctcgtgttc ctcggagtct
     7921 ttgaagccgt agccgaccgt gtttgtcagt atttcgtcca gcgacagcct tctggtttat
     7981 accgacggag tgacggaagc catgaattcc gcaggagagc tttttgggga acaacggctg
     8041 aagtatattc ttcatgattc aggaggcggt acggcgcaag aggaagtgcg tcatgtcctt
     8101 gcaaaaaccc gtgaatttgt aaacggagcg gtacagtccg acgatatcac catattggct
     8161 ttggaatatc tccggcgtgt tcaag
//
LOCUS       DATALY010000153         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_809625,
            whole genome shotgun sequence.
ACCESSION   DATALY010000153 DATALY010000000
VERSION     DATALY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809625"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..667
                     /locus_tag="VHR42_04540"
     CDS             41..667
                     /locus_tag="VHR42_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3026478.1"
                     /translation="MKTGTVIVAAGMSSRMKAFKPLLPLSGSTIIGTAIATLQAAGIQ
                     DIAVVTGNGAEKLEQYLSATGVTCLYNEQYETTDMFSSAKIGLGYQKDRCDRVFFLPG
                     DVPLFSRQSLVSMQNDMNDSRCEILLPVHKGKMGHPILLNSNAIPFLTDYRGGGGLKG
                     ALDSFSGLKRIIELNDVGMTLDADRPEDYEQLKAYVRRRSDGADTADV"
     gene            642..1154
                     /locus_tag="VHR42_04545"
     CDS             642..1154
                     /locus_tag="VHR42_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3026479.1"
                     /translation="MVPTRQMCEQMWEDKGLTDGLKKHLAAVADLAVRIGTALNQKGF
                     ALNIPLIEAAALLHDIEKGIPHHAVTGAKTLETLGFTEIAPIVKFHMRLPDGFQPEIT
                     ELTVVFLADKLFIGSQSVSLQKRYAEKLSAYQDDPPALQTIRKQLEISLNLEKRMKTV
                     LGVDSLILLS"
     gene            1169..2191
                     /locus_tag="VHR42_04550"
     CDS             1169..2191
                     /locus_tag="VHR42_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3026480.1"
                     /translation="MKQINTTEAVGHVLCHDITQIIPGEKKDAIFRKGHIVTEEDIPV
                     LLSIGKEHLFVWEKQEGMLHENEAAEILYSLCKNEHMSPTPVKEGKIDLIAETEGLFK
                     VDLDRLNRINALGEMMIATRHSNFAVHKGDKLAGTRIIPLVIDQAKMDRAKAVAWNEP
                     LFQLLPFGHKKAAIVTTGSEVFYGRIKDAFGPVIRGKLSEFDVELLGQTIVNDEKEKI
                     INAIKEYIGQGADLIVCTGGMSVDPDDSTPSAIKGTGARLITYGAPVLPGAMFLLAYY
                     GERNIPIMGLPGCVMYSKRTIFDLVLPRIMAGEILTAEDLSVLGHGGLCLGCPECTFP
                     NCGFGK"
     gene            2258..>2669
                     /gene="modA"
                     /locus_tag="VHR42_04555"
     CDS             2258..>2669
                     /gene="modA"
                     /locus_tag="VHR42_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3026481.1"
                     /translation="MKKILSILLVAGMLSLSGCSSGASSSSSSSSAASSTVSSSSASS
                     AATQTTLTIAAAASLTDVTKEIATQYKKVEPNVTLTFTYGSSGALQTQIEQGAPVDIF
                     MSAAAKQMDALDSKNLIDKNTRVNLLENKVVLITP"
BASE COUNT          717 a          657 c          706 g          589 t
ORIGIN      
        1 ccgattgacg gccgttatac cgacaaatgg gagttgtaag atgaaaacag gaacagttat
       61 tgtcgcagcc ggaatgtcct cgcgcatgaa agcatttaag ccgctgctgc cgctttccgg
      121 ctccaccatt atcggaaccg ccatcgctac cctgcaggcg gcggggatac aggacattgc
      181 cgtggttact ggcaacgggg cggaaaagct tgaacagtat ctgtcggcaa ccggcgttac
      241 ctgcctttac aacgaacaat atgaaacaac cgatatgttc agttctgcga aaatcggctt
      301 aggctatcaa aaagacagat gcgacagggt gtttttcctg cccggcgacg tgccgctgtt
      361 ttcaaggcag agccttgttt caatgcagaa cgatatgaat gacagccgct gtgaaatttt
      421 gttgcctgtg cacaaaggca aaatgggtca ccccatactg ttaaacagca atgcgattcc
      481 ttttctgaca gattaccggg gcggaggcgg ccttaaaggt gctcttgaca gcttcagcgg
      541 attaaagaga attattgagc ttaacgatgt cggcatgacc ctggacgcgg acaggcctga
      601 ggattacgag cagttgaaag cctatgtaag gagacgatcg gatggtgccg acacggcaga
      661 tgtgtgagca gatgtgggag gacaaggggc ttacagacgg cctgaaaaag catctggccg
      721 ctgtggcgga tctggccgtc cgaattggga cggcgcttaa ccaaaaaggg tttgcgctca
      781 atattcccct gattgaagca gccgctcttt tgcatgatat agaaaaagga ataccgcacc
      841 atgccgtaac gggcgcaaaa acgcttgaaa cgcttgggtt taccgaaatc gcgccgattg
      901 taaaattcca tatgcgcctg cccgacggat tccagccgga gataaccgag ctcactgtcg
      961 tgtttttggc ggacaagctg tttatcggct cacagtctgt cagcctccaa aaacgctatg
     1021 ccgaaaaact gtctgcctac caggatgatc ctccggcgct tcagaccatc agaaagcagc
     1081 ttgaaatttc attgaatctg gaaaaacgga tgaaaacggt tcttggggtt gacagtctaa
     1141 tattattatc atgaaaatgg agaagcatat gaaacagatt aacaccaccg aagcagtagg
     1201 gcacgttctt tgccatgaca tcacgcagat tatccccgga gagaaaaaag acgcgatttt
     1261 ccgcaagggg catatcgtca ccgaggagga catccctgtg cttctttcca tcggcaaaga
     1321 gcaccttttc gtctgggaaa agcaggaggg catgctgcat gaaaacgaag cggcggaaat
     1381 tctgtattcc ctgtgcaaaa acgaacatat gagccctacg ccggtaaagg aaggaaaaat
     1441 tgacctgata gctgaaaccg agggcctttt taaggttgat ttagaccggc tgaaccggat
     1501 taacgcgctc ggcgaaatga tgattgcgac gcgccactca aattttgcgg tgcataaagg
     1561 agacaagctt gccggaaccc gcataatccc gcttgtaatc gatcaggcaa aaatggacag
     1621 ggcaaaagcc gttgcgtgga atgagccgct atttcagctg ctgcctttcg gccataaaaa
     1681 agccgcgatc gttacaaccg gcagcgaggt gttttacggc cgcatcaagg acgcgttcgg
     1741 ccctgtaatc cgcgggaagc tgtccgaatt tgatgttgaa ctattgggac agaccattgt
     1801 aaacgatgaa aaagagaaaa tcatcaatgc gataaaagag tatatcgggc agggcgcgga
     1861 tctgatcgtc tgcacaggcg gcatgagcgt cgatcccgat gacagcaccc cttccgccat
     1921 taaaggaacc ggcgcgcggc ttatcaccta cggggcgccg gtgttaccgg gcgccatgtt
     1981 cctgcttgcc tattacgggg agcggaatat tccgattatg ggactgccgg gctgcgtaat
     2041 gtactcgaag cgcacgatat ttgatctggt tcttccccgg attatggcgg gggaaatact
     2101 cacggcggag gatttatcgg ttttgggaca cggcggttta tgccttggct gcccggagtg
     2161 caccttcccg aactgcggtt ttggcaaata aacgtcggaa gaaacgggca ttatatgaat
     2221 aagcgttaga caattaaata taaaggagaa atcagcaatg aaaaagattc tcagtattct
     2281 tctcgtggca ggcatgcttt ccctctccgg ctgcagctca ggcgcttcat cctcttcgtc
     2341 atcctcatcc gccgcatcct caaccgtttc ttcctcatcc gcgtcttcgg cagccacaca
     2401 gacaaccctg acaatagccg cggcggcaag ccttaccgac gttacaaagg aaatcgcgac
     2461 ccagtataag aaggtggaac caaatgtgac actaacgttc acctacggct cgtccggcgc
     2521 attacagaca cagattgagc agggcgctcc ggtcgacatt tttatgtcgg cggcagcaaa
     2581 acagatggac gctctcgatt caaagaacct cattgataaa aacacccgag taaacctgct
     2641 ggaaaacaag gtagtgctga ttactccgt
//
LOCUS       DATALY010000154         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_466612,
            whole genome shotgun sequence.
ACCESSION   DATALY010000154 DATALY010000000
VERSION     DATALY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466612"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VHR42_04560"
     CDS             complement(<1..675)
                     /locus_tag="VHR42_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX3026482.1"
                     /translation="MTFIPSKRVKTKTVSGLNENLSGDGSFLRLLRLMLPYKYRILFA
                     ALCVLMVNMAVIVKPFILKLVIDDFFVGHVAQKGFYTITTMGILYLLTALAGSGFSYL
                     QKNIINRVGQDIIRGLRSRLFKTIQYLPLRYLDRTSSGRLITRATNDVEALSQLYTDV
                     LLNLFQDIFLLIGIAYTMFALNFKLALLSFTVIPPMAAALFLLRKRIKRNFLRVKTLI
                     GRINGFM"
     gene            complement(623..2368)
                     /locus_tag="VHR42_04565"
     CDS             complement(623..2368)
                     /locus_tag="VHR42_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026483.1"
                     /translation="MAGRSIFNFIKKHAFSYIFGILFMIAASTIQTFFPKVLGHTIDL
                     LKLKNFNPADVKFNILLILLIAAATFVFTYLWRNLVIGNSRKLECDLRENFYSHLQKM
                     SAAFYSERKTGDLIAYGINDISAVRMAFGPTSALAINSLVICAASIYSMSVSINWRLT
                     LMALLPIPFIVFFTVKAGFAIKKRFKKVQESFGTISGRIQENIYGIRVIKAYVQEESE
                     IQNFEVLNRQMSDANLNLVKVSALLTPTIELCFSLSFVMTLIFGGSMVLHQVISLGDF
                     VAFNTYLVTIMTPIISIGGVINNYQRGMASLGRLNDIFNIPPEITEPTHASKDAIQGT
                     VEFRNLSFTYPGAQEKALDSINISLQKGHTLGVVGKTGAGKTTLASLLFRLYNVGDGQ
                     ILIDGKDINRISLKTLRNSISLAPQDNFLFSTTVRDNIRFFKEIFSEDDVENAARQGC
                     IYDHIEALDEGFGTVLGERGVNLSGGQKQRVSIARSLIKESPVLILDDTLSAVDTVTE
                     GRILENLWKVRRGKTNIIIAHRLSAVKNADEIIVLDHGRIVERGTHSQLIERKGYYYD
                     IYTEQTSKDENSLGS"
     gene            complement(2398..3162)
                     /locus_tag="VHR42_04570"
     CDS             complement(2398..3162)
                     /locus_tag="VHR42_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX3026484.1"
                     /translation="MDGYDLRLAVLIDAENVPYSNVKGMMEEITRYGTPTIKRIYGDW
                     TSRVVSGWKNVLVENAITPVQQYSYTTGKNSSDSAMIIDAMDILYAGNIDGFCLVSSD
                     SDFTRLATRLRESGKKVYGIGEKKTPGSFIAACDKFIYLEIINSSADAPEASAESAGE
                     TAPKPREISKVDRKVIKLITNSVTDLADENGWAFLAEVGSLIAKKQPDFDSRNYGFSK
                     LTRLVESIGKFELDVREKENHIKHIYIRNRDSGRKN"
     gene            complement(3247..3867)
                     /locus_tag="VHR42_04575"
     CDS             complement(3247..3867)
                     /locus_tag="VHR42_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEX3026485.1"
                     /translation="MSGNETVEKAIRRMLKERKTFESRYLAVSGLSKTANGELSGRQK
                     LAFEQYVQASYFNRIIQRANTRLDRMTDGRFELRRRESAADLRSQTGLELDVLDHYTG
                     KIRTVKSLSGGESFKASLSLALGLSDVIQSHAGGVEVDTMFIDEGFGSLDSESLEQAI
                     RTLCELTDGNRLVGIISHVSELKERIDRQVVITKSPTGSVIRLVSS"
     gene            complement(3854..>4330)
                     /locus_tag="VHR42_04580"
     CDS             complement(3854..>4330)
                     /locus_tag="VHR42_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026486.1"
                     /translation="ADKESAERVIQELQRRLDEMKKSLEAAEKAYIDVKSRLEGDRAV
                     LSDQLNRRETLAAAVLKARDDFLEKCAAYGFASEERYREAYLDESGLASLQKQVTDYR
                     SACTQAESELNRLNEETRDRKPQDTMVMQEEKRRIEEEKKAYRRGNPHAVGENERE"
BASE COUNT          947 a         1141 c         1130 g         1112 t
ORIGIN      
        1 catgaaaccg ttgatgcggc caatcagcgt tttgaccctg agaaagttcc gcttaattct
       61 tttccgcagc aggaataaag cggcagccat cggcggtatc accgtaaacg acagcagcgc
      121 gagcttaaag ttcagagcaa acatggtata tgcgatgccg atcaacagga aaatgtcctg
      181 aaacagattc agcagcacgt cggtatacag ctggctgagc gcttcgacgt cgttggtcgc
      241 cctggtgatc agacgcccgg aggaggtccg gtcaagatag cggaggggga ggtactggat
      301 ggttttgaaa agcctgcttc tcagcccacg gatgatatcc tgaccgaccc ggttgatgat
      361 atttttctga agataggaaa atccgctgcc tgcaagcgcg gtcagaagat acagaatccc
      421 catcgttgta atggtgtaaa agcctttttg cgcgacgtgg ccgacaaaaa agtcgtcgat
      481 cacaagcttg aggataaaag gcttcacgat aacggccatg ttgaccatca gcacgcagag
      541 cgcggcgaac agtatccggt atttataggg cagcatcagc cgaagcagcc tcaaaaagct
      601 tccgtcgccg gaaaggttct cattaagacc cgagactgtt ttcgtcttta ctcgtttgct
      661 cggtataaat gtcataataa taacccttcc tttcgattag ctggctgtga gtgcctcttt
      721 ccacaatcct gccgtggtcc agcacgataa tctcgtcggc gtttttgacg gcggaaagcc
      781 tgtgagcgat gatgatgttg gtttttcccc ggcgcacttt ccacaggttt tccagaattc
      841 tgccctccgt gaccgtatcc acagcggaaa gggtatcgtc gagaatcaga acgggagact
      901 ccttgatcag cgaccttgcg atcgaaacgc gctgcttctg cccgcccgac aggttgacgc
      961 cacgctctcc gagtacggtg ccgaagccct cgtcgagggc ttcgatgtga tcgtaaatgc
     1021 agccctggcg agcggcgttt tccacatcat cctcggagaa gatttcttta aaaaagcgga
     1081 tgttgtccct gacggtggtg gagaacagaa aattatcctg gggggctagg ctgatgctgt
     1141 ttcgcagggt cttcagggag atgcggttga tatccttccc gtcgatcaga atctgtccgt
     1201 ccccgacgtt atagagccgg aaaagcaggc tggcaagcgt tgtcttgccc gcgccggttt
     1261 tgccgaccac accgagcgtg tgcccttttt gcagtgaaat attgatggaa tccagcgcct
     1321 tttcctgtgc gcccggatag gtgaaactga ggttccggaa ttcgacggtc ccctgtatgg
     1381 cgtctttgct ggcgtgagtc ggctccgtga tttcgggcgg gatgttgaag atatcgttga
     1441 gccgcccgag cgatgccatt ccccgctgat agttgttgat gactccgccg atggagataa
     1501 tcggagtcat aatggtgaca agataggtgt taaaggccac gaaatcgccc agtgaaatga
     1561 cctgatggag caccatgctg ccgccgaaga tcagggtcat gacaaaactc aggctgaagc
     1621 agagctcaat tgtgggtgtc agcagtgccg ataccttcac taaattgaga ttcgcgtccg
     1681 acatctgccg gttgagtact tcaaagttct gaatctccga ctcttcctgc acataggctt
     1741 tgatcacgcg gatgccgtaa atattctcct gtatgcggcc ggatatggtg ccgaaggatt
     1801 cctgcacctt tttaaaccgt ttcttgatgg caaagcccgc ctttacggtg aaaaacacga
     1861 taaagggaat gggcagcagc gccatcagcg tcaggcgcca gttgatgctc acgctcatcg
     1921 agtaaatcga cgcggcgcag atcaccagac tgttgatcgc cagcgcggaa gtcggaccga
     1981 aagccatacg gacggctgaa atatcgttga ttccgtaagc gatcaggtcg ccggtttttc
     2041 tttcgctgta aaaagcggcc gacatcttct gaaggtgtga atagaagttt tcccgcaagt
     2101 cgcactccaa ctttcgggaa ttgccgatga cgaggttcct ccagaggtag gtgaagacaa
     2161 aggttgcggc tgcaatcagc aggatcagca ggatgttgaa tttcacatcg gccggattaa
     2221 aatttttcag cttcagcaga tcaatggtat gccccagtac ctttggaaaa aaagtttgaa
     2281 tcgtagacgc cgcgatcatg aacaaaatac cgaagatata ggaaaaagcg tgttttttga
     2341 taaaattgaa tatgctccga cccgccattg cgacacttcc ttatgttgcg gatccggtta
     2401 gttcttacgg ccggaatccc tgttgcggat atagatatgt ttgatatgat tttctttttc
     2461 ccttacgtcc agctcgaatt ttccgattga ttcgacaaga cgcgtaagct ttgaaaatcc
     2521 gtagttgcgt gagtcaaaat ccggctgctt ttttgcgatc aggctgccga cctcggcgag
     2581 gaaggcccag ccgttttcat cggcgagatc tgtgacggaa ttggtaatca gtttgatgac
     2641 tttccggtca actttggaaa tttccctggg tttcggggcc gtctctccgg cggactctgc
     2701 cgaagcttcc ggggcgtcgg cggaggaatt gataatttca aggtaaataa acttgtcgca
     2761 agccgcgata aacgatccgg gcgttttttt ctccccgatg ccgtagacct tcttgcctga
     2821 ttcgcgcaga cgcgtagcca gacgggtgaa atcactgtcg cttgagacca gacagaatcc
     2881 gtcgatgttg ccggcgtaaa ggatatccat cgcgtcgata atcatggctg agtcgctgga
     2941 gtttttgccg gtggtgtagc tgtactgctg cacgggcgtg atggcgtttt ccacgagcac
     3001 gttcttccac cccgatacaa cccttgaagt ccagtccccg taaattcttt tgatggttgg
     3061 cgttccatag cgcgtgattt cctccatcat accctttaca ttgctgtaag gtacgttttc
     3121 ggcgtcgatc agcaccgcca gccgtaaatc atatccgtcc attctttctc cattcctttc
     3181 cgcagccgcc atctatttat tctaaatgag aacactgttt ccactttaac gcttatgctt
     3241 tatgttttat gatgaaacca gccggatcac actgccggtc gggctcttgg taatgaccac
     3301 ctgccggtcg atacgctcct taagctcgct tacgtgagag ataataccga caagacggtt
     3361 tccgtccgtc agctcgcaca acgttcggat ggcctgctcg agagattcgg aatccagtga
     3421 cccgaagccc tcgtcgataa acatcgtatc cacttccacg cccccggcgt ggctctggat
     3481 gacgtccgac aggcccagcg caaggcttag tgaagctttg aaggattctc cgcccgaaag
     3541 ggatttgacg gtgcggattt tccccgtata gtggtcgagc acatcgagct cgaggcctgt
     3601 ctgggagcgt aagtctgcgg ccgactcgcg ccgccgcagc tcaaaacgcc cgtccgtcat
     3661 gcggtcaagc cttgtgttgg ctctctgaat gatccggttg aaataggagg cctgcacata
     3721 ctgctcaaaa gcgagtttct gcctgccgct cagttccccg ttggctgttt tggataaacc
     3781 gctgacggcg agataccggc tttcaaaggt tttccgctct ttcagcattc ttctgatcgc
     3841 tttttcaacg gtttcattcc cgctcattct cgcccacagc gtgcggattt cctcgtcggt
     3901 acgctttttt ttcttcttcg attcggcgct tttcttcctg catcaccatg gtgtcctgcg
     3961 gtttccggtc acgggtttct tcattcaggc ggttcagttc cgattcagcc tgggtacagg
     4021 cgctgcggta atccgtgacc tgcttttgaa gcgaggcaag cccggattca tccagataag
     4081 cctcccgata cctttcttcc gacgcaaacc cgtaggcggc gcatttttcg agaaaatcat
     4141 cgcgggcttt cagcactgcg gcggcgagag tctcgcgccg gttcagctga tcgctcagta
     4201 ccgcccggtc tccctccaga cggcttttca catcaatata ggccttttcc gctgcctcca
     4261 gtgacttttt catctcgtcg agccgacgct gcagttcctg aatcactctt tcggccgatt
     4321 ctttatcagc
//
LOCUS       DATALY010000155         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_810101,
            whole genome shotgun sequence.
ACCESSION   DATALY010000155 DATALY010000000
VERSION     DATALY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810101"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /gene="gatB"
                     /locus_tag="VHR42_04585"
     CDS             <1..1382
                     /gene="gatB"
                     /locus_tag="VHR42_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX3026487.1"
                     /translation="LATKTKIFCGCPTEFGGEPNTHCCPVCVGLPGTLPVLNEKVVEY
                     AVRAGLAMNCEIPHFSKLDRKNYFYPDLPKAYQISQYDLPLCKGGFVEIEGGKKVGIT
                     RIHIEEDAGKLVHDSAVRRTYVDYNRCGVPLIEIVSEPDMRGPEEVRDFVEKVRATLL
                     YAGVSDCRMEEGSLRADVNLSLRPVGAEKFGTRTEMKNINSIRAIYRAVEGEAKRQKE
                     LLESGGQVIQQTRRWDDNRGQSRVMRGKEEANDYRYFPEPDIVPVKLTDERIREIRSR
                     LPKMPGQRMGEYVSQGLSIADARTITASKALADFFEQAAESCGDAVECAKWIVGEMMR
                     HLKERELEPESIPFSAEHLASVIKMCREGKITAAAAKKVFGMLFEEKGTPEELVEKHG
                     LAVIGDDSALDAAIQNVLHANSKSVEDFKSGKEKAFGFIMGQCMKELRGKADPAVLRG
                     KLAARLNGE"
     gene            1709..>2695
                     /locus_tag="VHR42_04590"
     CDS             1709..>2695
                     /locus_tag="VHR42_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX3026488.1"
                     /translation="MENEKALSPAEIFGEYVFNDSVMRTRLSKGTYKTLRETIDSGLP
                     LNSTIADEIASAMKEWAVEKGATHFTHWFQPMTGITAEKHDAFISPAKAGKVVLEFSG
                     KELCKGEPDASSFPSGGLRDTFEARGYTAWDCTSPAFVKEGTLYIPTAFFSYTGEILD
                     KKAPLLRSVEALSKQALRILAAFGHTSSKKVIATVGPEQEYFLVNRAFYDKRLDLILT
                     GRTLFGAKPPKGQELEDQYFARLRIKITDFMHDLDQELWKLGITAKTKHNEVAPAQHE
                     LAIIFDPASVAADHNQLVMEMMRKIAKRHGLAALMHEKPFAGVNGSGKHINWS"
BASE COUNT          719 a          712 c          687 g          577 t
ORIGIN      
        1 agcttgccac gaaaacaaaa attttctgtg gctgccccac cgaattcgga ggagaaccga
       61 acacccactg ctgtccggtc tgcgtgggtc ttccgggaac gcttccggtt ttaaatgaaa
      121 aagttgtgga atacgcggta agagcagggc tcgcaatgaa ttgcgaaata ccgcattttt
      181 caaaactcga ccgtaaaaac tacttttatc ccgacctgcc gaaagcctac cagatttccc
      241 aatatgacct gccgctctgc aaaggcggct ttgtcgagat tgagggcggc aaaaaagtcg
      301 gcatcaccag aatccacatt gaggaagacg cgggcaagct cgtccacgac agcgccgtcc
      361 gacggactta tgtcgattat aaccgctgcg gagttcctct gattgaaatc gtttcggaac
      421 ctgacatgcg cggccccgag gaagtcaggg atttcgtcga gaaagtccgc gcgacattgc
      481 tttacgcggg agtttccgac tgccgaatgg aggaaggctc gcttcgtgcg gacgtcaacc
      541 tgtcactgag gccggtgggc gccgaaaaat tcggcacccg gaccgaaatg aaaaacatca
      601 actccatccg ggccatttac cgggctgtgg aaggggaagc caaacgccag aaggagctcc
      661 ttgaatccgg cggtcaggtt attcagcaga cccggcgctg ggatgacaat aggggccaaa
      721 gccgcgtaat gagaggcaaa gaggaagcca acgactaccg gtattttcct gagccggata
      781 tcgttcctgt gaaactcacc gacgagcgca tccgcgaaat tcgttcccgg cttcccaaaa
      841 tgcccggtca gaggatgggc gaatacgtgt cccagggcct ttcgatagcc gatgcgcgca
      901 ccataaccgc atccaaggcc cttgccgatt tctttgagca ggcggctgaa agctgcggcg
      961 acgccgtcga atgcgccaaa tggatcgtcg gcgaaatgat gcggcacctc aaggagcggg
     1021 agctggaacc cgaaagcatt cccttctccg ccgaacatct tgcctcggtc attaaaatgt
     1081 gccgggaggg taaaatcacg gctgcggccg caaaaaaggt attcggcatg ctgtttgaag
     1141 aaaaaggaac gccggaagaa cttgtggaga aacacgggct cgccgttatc ggcgatgatt
     1201 ccgcccttga tgcggcaatc caaaacgttc tgcatgcaaa ttcaaagtcg gtcgaagatt
     1261 ttaagtcggg caaggaaaaa gcctttggat ttatcatggg ccaatgcatg aaagagcttc
     1321 gcggcaaggc cgacccggca gtgctgagag gaaaactggc cgcacggctt aacggagaat
     1381 aatcaacccg gatacatcgt atgatttatt tctcagcgga catgaattat aggcaaaaaa
     1441 agtcgaaggg cacgtcctga atttaagcgt tcctacaaaa aatgaaatct taaaaaatca
     1501 acttgcatgg aaaaatttca tatgctataa taatcccata ggaacagagc ttcctcaaaa
     1561 agttgacacc gcgagagtct gcggaaaaga gctttgcctc agccgtggat ttccaaaccg
     1621 aaacaatgac gtttcgtggc tgccacagcc gcgttgcgtc tttttttgtt tgctttttac
     1681 catttactat tattaaggag gaaacaacat ggaaaacgaa aaagcacttt ctccggctga
     1741 aatattcgga gagtacgtct tcaacgactc cgtgatgaga acccgacttt caaaaggcac
     1801 ttataaaaca cttagggaaa ccattgattc cggcctgccg ctcaattcca caatcgccga
     1861 cgagattgct tcggcaatga aggaatgggc tgttgaaaag ggcgccaccc attttaccca
     1921 ctggttccag ccgatgaccg gaattactgc tgagaagcat gacgctttta tttccccggc
     1981 caaagccgga aaagtggttc tcgaattttc aggcaaagaa ctgtgcaaag gtgaacccga
     2041 cgcctcctcc ttcccgtccg gaggcctgcg cgataccttt gaggcgagag gctatactgc
     2101 ctgggactgc acctctcccg cattcgtcaa ggaaggcacc ctttacattc caaccgcatt
     2161 tttctcctac accggcgaaa ttcttgacaa aaaagctccc ctgctccgtt cggttgaagc
     2221 actgagcaaa caggccctgc gcattcttgc cgctttcgga catacctctt ccaaaaaagt
     2281 tattgccact gtcggcccgg agcaggaata ttttcttgtc aaccgcgcct tttatgataa
     2341 gcgtcttgac ctgattctga ccggccgcac cctgttcgga gccaaacctc cgaagggtca
     2401 ggagctggaa gatcagtatt ttgccaggct tcgcattaaa atcaccgact ttatgcacga
     2461 cctcgatcag gaactctgga aacttggcat caccgccaag accaaacata atgaggtcgc
     2521 tccggctcag catgagctgg ccatcatctt cgaccccgcc agcgttgccg cggatcacaa
     2581 tcagctggtc atggaaatga tgcggaaaat cgcgaagaga cacggtctcg ccgccttgat
     2641 gcatgaaaaa ccgtttgcgg gcgtaaacgg aagcggtaag catatcaact ggtcg
//
LOCUS       DATALY010000156         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_380062,
            whole genome shotgun sequence.
ACCESSION   DATALY010000156 DATALY010000000
VERSION     DATALY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380062"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VHR42_04595"
     CDS             <1..501
                     /locus_tag="VHR42_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX3026489.1"
                     /translation="EAAMIGMKLAPGLGRRFSAEAWGCLDGAIWERARSEALSAVDRT
                     PFEVYGYDIDEKAVELTLENSRKAGVGGFVRAKKQEIAQFAPEQSRGTVVCNPPYGER
                     MLEVRQAEEIYQTMGKVFLGLKNWNFYIISPSERFESLFGKKADKKRKLYNGMIKCEL
                     FQYFKR"
     gene            513..1460
                     /locus_tag="VHR42_04600"
     CDS             513..1460
                     /locus_tag="VHR42_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX3026490.1"
                     /translation="MESSLRHVFIVNPTAGKGNSLYFINYAVSNLLKGKVEGLSVRVT
                     GGPMDAAKIAAEEASRGDAVRLYACGGDGTLNEVANGAMGFDNAQIAPVPLGSGNDYV
                     KTFGGRAGFLDLAALMEGSVIKADMIQCNGRYAINLCSMGFDSSVASNMARFKRLPLV
                     NGSMAYDFSVVFSLLNNIRSSFQIVINDTDIYEGEFLLAVVANGKWYGGSYLAAPDAS
                     PFDGKLDFVLVRRVSRLKFLRLVDAYKRGEHEKLYDILHHYTGEKLTIRSRKPFPVNM
                     DGETIHVSDAEFRVVPGAFNLVVPPKLVNTYKNTREICI"
     gene            1602..1886
                     /locus_tag="VHR42_04605"
     CDS             1602..1886
                     /locus_tag="VHR42_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX3026491.1"
                     /translation="MTMKPLADRVVIKMVEAEETTKGGIILAGSAKEKPQIAEVIAVG
                     PGGLVDGKEVKMYVKVGDRVITSKYSGTEVKMDGTEYVIVRQSDILAVVE"
     gene            1913..3550
                     /gene="groL"
                     /locus_tag="VHR42_04610"
     CDS             1913..3550
                     /gene="groL"
                     /locus_tag="VHR42_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354131.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX3026492.1"
                     /translation="MAKDIIYSEEARKALQTGVDKLANTVKITLGPKGRNVVLDKKFG
                     SPLITNDGVTIAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATLLAQALIREG
                     MKNVAAGANPMVIKKGIQKAVDAAVNAVKSNSKKINGSTDIASAATISAGDEFIGKLI
                     AEAMEKVSSDGVITVEESKTADTYSEVVEGMQFDRGYVSPYMATDTDKMEAVVDDALI
                     LITDKKISNIQDILPLLEQIVQAGKKLVIIAEDVEGEALATLLVNKLRGTFTCVAIKA
                     PGFGDRRKEMLRDIAVLTGGEVISEEIGLDLKDATMNQLGHARQVKVLKENTIIVGGG
                     GDSAEIKSRIGQIRNQIESTTSDFDREKLQERLAKLSGGVAVIKVGAATETEMKEKKL
                     RIEDALAATKAAVEEGLVAGGGTALINAIPAVRALLENNTESDEKTGINIVVKSLEEP
                     LRQIAANAGVEGSVIIDKIINSGKVNYGFDAYKETYVDMFEAGIVDPTKVTRSALQNA
                     ASVAAMVLTTESLVADKKEKDAPAPNPAGMGGMGGMY"
     gene            3755..4153
                     /locus_tag="VHR42_04615"
                     /pseudo
     CDS             3755..4153
                     /locus_tag="VHR42_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619907.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
     gene            complement(4149..4423)
                     /locus_tag="VHR42_04620"
                     /pseudo
     CDS             complement(4149..4423)
                     /locus_tag="VHR42_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741431.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); incomplete; partial in the middle
                     of a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
BASE COUNT         1186 a          974 c         1229 g         1034 t
ORIGIN      
        1 gaagcggcca tgatcgggat gaagctggcc ccggggctgg gacggcgttt ttcagccgag
       61 gcctggggct gtctggacgg agccatctgg gaacgtgcgc ggagcgaggc gttgtcggcc
      121 gtcgaccgca ccccgtttga agtgtatggt tatgacattg atgaaaaggc cgttgagctg
      181 acgctggaga attcaagaaa agccggggtc ggcggatttg tccgcgcaaa aaagcaggag
      241 attgcgcagt ttgctcccga acagtcacgg ggaacggtgg tctgcaatcc tccttatggg
      301 gaaagaatgc tggaagtccg gcaggccgag gaaatttatc aaacgatggg aaaggttttt
      361 ttagggctga aaaactggaa cttttatatc atcagcccca gcgagcgttt tgaaagcctg
      421 tttggcaaaa aagcagataa aaagcggaag ctttacaacg gcatgatcaa atgcgaactg
      481 tttcaatatt ttaaacggtg aggtctaaag ccatggagag cagtctaagg catgtgttta
      541 ttgttaaccc cacggcgggg aagggcaact ccctttattt tatcaattac gccgtatcca
      601 atctgttaaa gggaaaagtc gaaggattgt ctgtccgggt gaccggcggg ccgatggatg
      661 cggccaagat cgcagcggaa gaagcatcca gaggcgacgc tgtgcgccta tatgcctgcg
      721 gcggagacgg aacgcttaac gaggtggcca acggcgcgat gggtttcgac aacgcgcaga
      781 tagcccccgt tccactcggt tcaggaaatg attatgtcaa gacctttgga gggagagccg
      841 gtttccttga tctggccgct ctgatggagg gaagcgtaat caaagccgat atgatccagt
      901 gcaacggccg ctatgccatc aatctctgtt ccatggggtt tgattcctct gttgcgagca
      961 acatggcgag attcaagagg cttcctctgg ttaacggcag catggcctat gacttttctg
     1021 tcgtgttttc cctgctgaac aatatccgca gcagttttca aatcgttatt aatgacaccg
     1081 atatctatga gggcgaattt cttctggcgg tagtggcgaa cgggaaatgg tacggcggaa
     1141 gctacctggc cgcgccggat gcgtcgccgt tcgacggaaa actcgatttt gtgctggtca
     1201 gacgtgtttc caggctgaag tttctccggc tggtcgacgc ttataaaagg ggcgagcatg
     1261 aaaagcttta tgatatactt caccactaca cgggggaaaa attaacgatc cggtcccgca
     1321 agccttttcc cgtgaatatg gatggtgaaa caatccatgt gtccgatgcg gagtttagag
     1381 ttgttccggg cgctttcaat ttagttgtac cgccaaaatt ggtaaatact tataaaaata
     1441 ctagagaaat ttgcatatag atgataaaaa ttcacgaaaa tgaaaacttt acaagtttag
     1501 cacttgattt ttagagtgct aatgattaaa atagttttag cactcaagaa taaagagtgc
     1561 taatttcaat ttaaattaac acagttcagg gaggaatagc aatgacaatg aagcctctgg
     1621 ctgacagggt ggttattaaa atggtagagg cagaggaaac caccaaaggc ggaatcattt
     1681 tggccggatc agcgaaggaa aaaccgcaga ttgcggaagt gattgcggta ggacccggcg
     1741 gtttggtcga cggcaaagaa gtcaagatgt atgttaaggt cggggatcgg gttatcacaa
     1801 gcaaatattc cggtacggaa gtcaagatgg acggaacgga atatgtgatt gtgcgtcaaa
     1861 gcgatattct tgctgttgtt gaataagacg gtaaacggag gtaatttgga ctatggcaaa
     1921 agatatcatt tattcggagg aagcacgcaa agcgttgcag accggtgttg acaagcttgc
     1981 gaatacagtt aaaatcacgc ttggccccaa agggcgcaat gttgttctcg ataaaaaatt
     2041 tggctcacct ctgattacga acgacggtgt taccattgcc aaagagattg agcttgagga
     2101 tccttttgaa aacatgggcg ctcagctggt aaaggaagtc gctaccaaaa caaacgacgt
     2161 agcgggtgac ggcaccacca cggcaactct gctggctcag gcactgattc gcgaaggcat
     2221 gaaaaatgtg gcagccggcg caaatccgat ggtcattaaa aagggtatcc agaaagcggt
     2281 ggatgctgcg gttaatgcag tcaaatccaa ttcgaagaag atcaacggca gcaccgacat
     2341 cgccagcgcg gccaccattt cggccggcga tgaattcatc ggtaagctga ttgccgaagc
     2401 gatggagaag gtctccagcg acggcgtgat taccgttgag gaatccaaga ccgccgatac
     2461 ctattccgag gttgtcgaag gaatgcagtt tgaccgcgga tatgtttcgc cctacatggc
     2521 gaccgatacg gataaaatgg aagcggtcgt tgacgatgcg cttatcctga tcacggataa
     2581 gaaaatttcc aatattcagg atatccttcc tctgcttgag cagattgtgc aggcgggcaa
     2641 aaagcttgtt attatcgcag aagacgtcga gggcgaagcc ctggcgactc tgcttgtgaa
     2701 caaactgcgc ggaaccttta cctgtgtcgc gatcaaagct ccgggctttg gcgacaggcg
     2761 taaagagatg ctccgtgaca tcgcggttct aacgggcggc gaggtcattt ccgaagagat
     2821 tggtctcgat ctgaaagatg cgaccatgaa tcagcttggc catgcgagac aggtcaaggt
     2881 tctcaaagag aataccatca ttgtcggcgg cggcggggat tctgcggaaa tcaaatccag
     2941 aatcggccaa atccgcaatc agattgaatc cactacttcc gatttcgacc gtgaaaaact
     3001 tcaggagcgt cttgccaaac tttccggcgg cgtagctgtc attaaagtcg gcgcggcgac
     3061 cgaaaccgaa atgaaagaga aaaagctccg tattgaggat gccctcgcgg ctaccaaggc
     3121 tgccgttgag gaaggccttg tagcaggcgg cggcacggcc ctgatcaatg cgattccggc
     3181 tgttcgtgct ttgcttgaga ataatactga gagcgatgag aagacgggta tcaacatcgt
     3241 tgtcaaatcc cttgaggagc cgcttcgcca aattgcggcc aacgcagggg ttgaaggctc
     3301 ggtcattatt gacaagatca tcaattccgg caaggtcaac tacggctttg acgcctataa
     3361 agagacctat gtcgatatgt ttgaagccgg tatcgttgac ccgactaagg ttacccgcag
     3421 cgcactgcaa aatgcggcgt cggtcgcggc tatggtgctg actaccgaat cgcttgttgc
     3481 cgacaagaag gaaaaggatg ctccggctcc taatccggcg ggcatgggcg gaatgggcgg
     3541 aatgtattaa ttcctgcccg gtgatcccga aagacagaaa aaacgcagga tttatcctgc
     3601 gtttttttga tttccggttg attaattctc cttccgccat tataattgtt tataaaacgc
     3661 ctgctgaata cagacatttg gaataaatcg ctctgaggtt cgcaggtcta ccataatgat
     3721 tacaccgcga aagcggagaa tggatgtata aaggatggct gaagacaagc aaaaaaaaat
     3781 ggtggaagcc atcacctcga tggatgatga ttttgccaaa tggtacacag acatcgttaa
     3841 aaaggctgac ctgattgatt attcaagcgt cagaggctgc atggtgatac ggccgtacgg
     3901 ttacgcaatc tgggagaata tccagaaggt tctggacggt aagtttaaac agactggtca
     3961 tgaaaatgtc tatatgccga tgtttattcc ggaaagcctg ctccagaaag aaaaagaaca
     4021 tgtggaaggc tttgcgccgg aagtggcgtg ggtgacaatg ggcggagacg aaaagcttac
     4081 tgagcggctt tgcgtacgcc cgacctccga aacgctgttc tgcgagcatt acgctcatat
     4141 tatacagagc tatcgcgccc ggtgaactgg atgccaaagg ctctggcaaa gccgtcacca
     4201 aaataatgcg atgttccgct ctgaagagcc ttaccgtcgt gcatcagtga ctcgatcgta
     4261 taagtagcca ccgctccggc aaacttctct ttatcggttt tgcgtccttt tatgacggga
     4321 attgcaaggt atttttcaca aaaatccgcg tagatgttca acatgcggat ggtctcatcc
     4381 tcggcctgct cggctgtctc gtgcatggtg tggccttcct gcc
//
LOCUS       DATALY010000157         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_296248,
            whole genome shotgun sequence.
ACCESSION   DATALY010000157 DATALY010000000
VERSION     DATALY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296248"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VHR42_04625"
     CDS             complement(<1..446)
                     /locus_tag="VHR42_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX3026493.1"
                     /translation="MSKQKRAFGPKNFHSKPNTEFTVTEPISLFDFLLLKLSNKSRNY
                     VKSLLTHGEIFVDGRQTTQYDTVLQEGQKIRINQTAVREKEQIGTLEILYEDSDLIVV
                     NKPAGLLTIASDKEKTATAYHLLTDYVRQKDPRSRIFIVHRLDRDTS"
     gene            complement(651..3308)
                     /locus_tag="VHR42_04630"
     CDS             complement(651..3308)
                     /locus_tag="VHR42_04630"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEX3026494.1"
                     /translation="MKEFLGTRENLTNQFHTDPMLGLSVGQAAQNQEKYGRNTLTKEK
                     SKSLTKRIFEASVEPMILMLIMAGIIDIAVNIIRASTGGEADFLECIGIFVAISLSVV
                     ITVVMEGKSAKAFEALSKIGEDATVKAIRDGETIILSQKDLAVGDIVLLATGDKIPAD
                     GRLLEGVGLESDESALTGESVPVKKDAETILTDEKTPLAERSNMLYSGNYITNGYGKM
                     MITAVGDSTEFGKIAKELTQTGRSSTPLQEKLAKLGKIITILGVIAAAIVFITELISF
                     ARSGQLMFEKVLEAFVTSIVLIVAAVPEGLPTIVAVSLSINIIKLSRQNALVKKMIAS
                     ETVGCINVICSDKTGTLTENKMTVPSFYDGKWHEKTSELSSDWMVHNICLNTTADVSQ
                     DGTFIGNPSECAMLNFYEGSEARKKNGKSYREERSDHDVLMAFPFSSELKHMTTISKV
                     DGRIISYVKGSPESIFSMCVVSDSQRKEIEHYITAVQEKAMRVIAFAHKELDTMRDYE
                     DERLHATMETGMTFDGFVAISDPLRTDVYDAVNNCRRAGIDLKMLTGDNIVTASAIAS
                     ELHILGEGRIAVEAKNIVDLSDEELLKLLPKISVIARSTPTIKMRVVKLLKAQGNVVA
                     VTGDGINDAPALKNADVGIAMGISGTDVSKEASDIVLLDDSFSTIVKAIAWGRGIYEN
                     FKRFIQFQLTVNVASVIVVFTSIILGFKAPFTALQLLWINIIMDGPPALTLGLEPIYG
                     DLMNRRPTRRNESIVSKPMLLRIALTGVYMSVIFLCQYAFNFLGAAPRTMTTVLFTLF
                     TLFQLFNAFNCRELHTVSIFKHLLKNKIMLLVISITFLLQIVIIQFAGAFFGTVPLDL
                     TMWLKIFGVSFSVVLLSEIIKFVRRKAIR"
     gene            complement(3668..4006)
                     /locus_tag="VHR42_04635"
     CDS             complement(3668..4006)
                     /locus_tag="VHR42_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3026495.1"
                     /translation="MQSDELIQFRNVYNSVMNESDDFYKNFGKLDDDAYSDGAIPKKN
                     KELMGLAISVVTRCNECILYHLGGCVDAGAKKDEILEAIKIGVIGGGSITYPNVRFAF
                     QMMKELKIID"
     gene            4445..4579
                     /locus_tag="VHR42_04640"
     CDS             4445..4579
                     /locus_tag="VHR42_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPC_1213 family protein"
                     /protein_id="HEX3026496.1"
                     /translation="MGNVKKQDKKEDGKFHSKHINHDSRTESAKAVFDSNDKNANKSK
                     "
     gene            complement(4653..>4960)
                     /locus_tag="VHR42_04645"
     CDS             complement(4653..>4960)
                     /locus_tag="VHR42_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEX3026497.1"
                     /translation="HLLNEIAERKKAYQKKNVLLLVNSPEAMRDATLQRTLKAVPTLR
                     VFVCKEDSFPLRLFEELELGDHRLPLSAVLGQNPKARFAFSNYNVGTAELLLQISDF"
BASE COUNT         1323 a         1147 c         1191 g         1299 t
ORIGIN      
        1 gaggtgtcgc ggtcaagtcg atgtacgata aaaatacggc tgcgggggtc cttctgcctt
       61 acataatccg tcagcaagtg gtaggctgtc gctgttttct ctttatcgga cgcgattgtg
      121 agcagaccgg ctggtttatt gactacgata agatcgctgt cttcatacag aatttccagc
      181 gtgcctatct gctctttctc tcgaaccgcg gtttgattga tgcgtatttt ttgtccttcc
      241 tgtaagacgg tatcatattg agtcgtctgt cgcccatcga cgaagatttc tccgtgggtc
      301 agcaaagact ttacgtaatt ccttgatttg tttgataatt tgagcagtaa aaaatcaaat
      361 aagctgattg gttccgttac ggtaaattcc gtattaggtt tggaatgaaa gttttttggc
      421 ccgaatgcgc gtttttgttt gctcacaagt tttctccttt gatttgacac tttccttcac
      481 ggagtcaggt acaaatcgtg taaaaaatga tccaattcag tgtaacataa aaatttctgg
      541 aatagaagac ataaaaacgt atccgcagaa tgaggaaggg gaatcagcag gataataacg
      601 gctgaccacg aaagtggtca gccgttttca gggtaagaac tatcagccgc ttagcggatg
      661 gcttttctgc ggacgaattt aatgatttcc gataacagaa cgacgctgaa cgaaacgccg
      721 aagattttca gccacatcgt caggtcaagc ggaacggttc cgaaaaaggc ccctgcaaat
      781 tgaataatta cgatttgcag aaggaaggta atcgaaataa ccagcagcat aattttgttt
      841 tttaataggt gcttaaaaat gctgaccgta tgaagctccc ggcaattgaa ggcgttgaac
      901 agctgaaaaa gagtaaatag agtgaacaga accgtcgtca tggtcctcgg tgccgcgcct
      961 aagaaattga aagcatactg gcacagaaaa atcacggaca tataaacacc ggtcaatgca
     1021 atccgtaaaa gcatcggctt ggaaacgatg ctttcgtttc ttctggtggg ccggcggttc
     1081 atcaggtcgc cgtaaatcgg ttccaaccca agggtaagag ccggagggcc atccatgatg
     1141 atgttgatcc acagaagctg aagtgccgta aacggggctt tgaacccgag aatgatggag
     1201 gtgaacacga cgataacgga agccacgtta accgttaact ggaactggat aaaccgcttg
     1261 aaattctcgt agatgccgcg tccccaagcg atcgccttga caatcgtaga gaacgaatcg
     1321 tccagcagaa caatgtcgct ggcctctttt gatacgtcgg tgccggagat tcccatcgcg
     1381 atgccaacgt cggcattttt taatgctggg gcgtcgttga ttccgtctcc ggtaacagcg
     1441 actacattac cctgtgcttt gagcagcttg actacccgca ttttgatggt gggcgtgctc
     1501 cgggcgatga cactgatttt cggcaggagc ttcaagagct cctcatcgct gaggtcaacg
     1561 atatttttcg cttcgacggc gattcggccc tcacccaaaa tgtgaagctc actggcgatc
     1621 gcggatgcgg taacgatgtt gtcgcccgtg agcattttca ggtcgatacc ggcacgccgg
     1681 cagttgttta cagcgtcata cacgtcggtc cgaagcgggt ccgaaatggc aacgaaaccg
     1741 tcgaaggtca tgccggtttc catggtggcg tgaaggcgct catcttcata atcgcgcatc
     1801 gtatccagct ccttgtgagc gaacgcaata acgcgcatgg ctttttcctg aacggcggta
     1861 atatagtgct caatttcctt tcgctgcgaa tcggaaacga cgcacatgga gaagatggat
     1921 tccgggcttc ccttaacata ggaaatgatt cttccgtcta ctttgctgat ggtggtcatg
     1981 tgtttgagct ctgaggaaaa tggaaaggcc attagcacat cgtggtcgga gcgctcttcc
     2041 cgataggatt tcccgttttt ctttcgggct tcggatcctt cgtagaaatt cagcatggcg
     2101 cattccgaag ggttcccgat aaaagtgccg tcctgtgaaa cgtccgccgt cgtgttcagg
     2161 cagatattgt gaaccatcca atcggaggac agctccgacg tcttctcatg ccattttccg
     2221 tcataaaagg agggaaccgt cattttgttt tcggtcagcg taccggtctt atccgaacag
     2281 attacattga tacagcccac ggtttcggac gcgatcattt ttttgaccag tgcgttctgc
     2341 cgggagagct tgataatgtt aatagagagc gaaaccgcta caatcgtcgg aagaccctcc
     2401 ggcacggcgg cgacaatcag gacgatgctg gtgacaaagg cttccagaac cttttcaaac
     2461 atcagctggc ctgaccgggc aaaggaaatg agctccgtga taaatacaat ggccgcggcg
     2521 atcacgccta agatggtaat gattttgccg agctttgcaa gcttttcctg aagcggtgtg
     2581 gaggagcggc cggtctgggt gagctccttt gcgattttcc cgaactcggt gctgtcgccg
     2641 acagcggtga tcatcatttt gccgtatccg tttgtgatgt aattgccaga gtaaagcata
     2701 ttactgcgtt ccgccagcgg cgtcttttca tcggtcagaa ttgtttccgc gtcttttttt
     2761 accgggacgc tttcgccggt tagtgcggat tcatcggatt ccaacccaac gccttccaaa
     2821 agccttccgt cggcgggaat cttatcaccg gttgcgagca gcacgatgtc tcccaccgca
     2881 agatcttttt gactgaggat gatggtctcc ccgtcgcgaa tcgccttcac agtggcgtct
     2941 tcgccgattt tggagagagc ctcaaaagcc tttgcgcttt tcccctccat cacaacggta
     3001 atcacgacgg agagcgagat ggcgacgaag attccgatgc actccaaaaa atccgcctcg
     3061 ccgccggtag acgcgcgtat aatattgacc gcaatgtcga tgattcccgc cataatcagc
     3121 atcagaatca ttggctcaac ggaagcctca aaaatgcgtt ttgtcaaaga tttcgatttt
     3181 tccttggtga gcgtattcct cccgtatttc tcctgattct gtgccgcttg cccgacactc
     3241 aatcccaaca ttgggtccgt atgaaactgg tttgtaagat tttctcgggt tcctaaaaat
     3301 tccttcactc gaattcctcc ttgtacaaat tctctgcatc ctgcaacgtc tttatacaaa
     3361 acaaaagggg cccgcatatg gcgatatacc atatgcgggc ctgaataaag accacgcatg
     3421 gcaaaaaagc catacgtgag tctcgttttt taagacaagc cagaccttta cggtcaggat
     3481 gttgacttga cacgcagtga tttgcgcaaa ctactccctc acgagcattt tgaattgtta
     3541 gcgtattata acatttcagc gaggcatagt caagatgaaa acagtcgatt tttcatgtgt
     3601 tagggaaaag acagtcggca atcaggaatt atgatcgcaa aaacaaatgg ctttcccgat
     3661 tctgcactca gtcgattatt tttaactctt tcatcatttg aaaagcaaaa cgcacattgg
     3721 gataggtaat cgagccaccg ccgatcactc cgattttaat cgcttccaat atctcgtctt
     3781 tctttgcgcc cgcgtcgacg cagccgccca aatgatataa gatgcactcg ttgcaccttg
     3841 tgacaacaga gatggctaat cccatcagct ccttgttctt tttgggaata gcaccgtcgc
     3901 tgtaagcgtc gtcgtctaat ttgccgaaat ttttgtaaaa gtcatcactt tcgttcataa
     3961 cggaattata cacgttgcga aattgaatca attcatctga ctgcatagat acattcctcc
     4021 attattctga ttttggcgag tgaaacaaaa caaaacaaac aaaaaactag tacgatgtca
     4081 ggcaaagggc aaaaacaaat caatcatata ttgtagcctc gcttaaccta agattcaagt
     4141 catacaagaa ttatagaagt tatgggcttc tgctttgagc tttgttcgat caggcatgaa
     4201 ccttttttga ttgttcgata aaaggcctta gacagcagaa gtagaataaa tgaaattcgc
     4261 aatggcacaa taattccgag gtgaggaaaa tggctggaaa gcattcggaa aacccatccg
     4321 ggttccagaa aaagttgaag gatgccgttg catctgaaaa ggccaaggaa gcaaatctga
     4381 atcaaaatca caacacaaag aaagtttctc ttggaccgaa caccaaccga taggggagaa
     4441 gcatatgggc aatgtaaaaa agcaggataa aaaagaggac ggaaagtttc attccaagca
     4501 tattaaccat gattcgcgca cggaaagcgc aaaagccgtg tttgattcaa acgataagaa
     4561 tgcaaacaag agcaaataaa aatcactcct gtcgcaagct tttctgcggc aggagtgatt
     4621 tgtcatatct atataaaggg cgatggaaac gatcagaaat cggaaatctg cagaagaagc
     4681 tctgcggttc ctacgttata gtttgaaaac gcgaatctgg cttttggatt ttgacccagt
     4741 actgcggaga gcggcagtct gtggtcaccc agttccagtt cttcaaacag gcggagcggg
     4801 aaagagtctt ctttgcagac aaaaacccgc agagtcggca cagccttcag agtgcgctga
     4861 agggtggcgt ctcgcattgc ttcgggactg ttgaccagaa gaaggacgtt tttcttttgg
     4921 taagcttttt ttcgctctgc aatttcattg agcaaatgct
//
LOCUS       DATALY010000158         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_811120,
            whole genome shotgun sequence.
ACCESSION   DATALY010000158 DATALY010000000
VERSION     DATALY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811120"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            582..1457
                     /locus_tag="VHR42_04650"
     CDS             582..1457
                     /locus_tag="VHR42_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX3026498.1"
                     /translation="MVDFIIAAASTADLPAEYFAEHDVPVIRYTYIMDGKPFEDDCKE
                     ETREKIYKRMREGAVMTTSMINTYTYYDFFKQLLDTGKDVIFLDMTRQISHSYSNAEK
                     TAEQIKTEYPKQRFYLMDTLCVSGGLGLLLHYMVNLRDAGKSFDETIKWAEANKLKIM
                     HWFTVGDLNYLKRGGRVSNAAAMVGSLLSIKPVLYVSEDGKLVVANKIRGRKAALVEI
                     LDRMKKDFTEPDGKEVFINHADCLEDAEFMRDQILETFPAVSKVTIRNLGVVIGAHCG
                     PGLLAIFYLGNKRYA"
     gene            complement(1554..1988)
                     /gene="ruvX"
                     /locus_tag="VHR42_04655"
     CDS             complement(1554..1988)
                     /gene="ruvX"
                     /locus_tag="VHR42_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX3026499.1"
                     /translation="MKILAVDLGRARTGLAICDESELLASPAGVISEYNMERLAQTVA
                     DQAGERAAGCIVVGLPRNMDGSEGESAQNARAFAENLQKLVSVPVVMRDERGTTITAH
                     GYLNTTDTRGKKRKAVIDAVAATIILQDYLDYRRNQKNNGGL"
     gene            complement(2006..2464)
                     /locus_tag="VHR42_04660"
     CDS             complement(2006..2464)
                     /locus_tag="VHR42_04660"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3026500.1"
                     /translation="MARPVRARPKSTASIFIAFLRSAYSWDELPSIYPEEIEVWNKRP
                     YDFTPSGGETMREVYNRMWECVTDIVRGNQGKTVCVTSHGCAIRNFLCHALNKPIEQI
                     NQVGWCDNTAISQIDFDKKLHSHVVMMNDASHLTPETSIFMHQTWWLPQE"
     gene            2415..>2525
                     /gene="ruvX"
                     /locus_tag="VHR42_04665"
     CDS             2415..>2525
                     /gene="ruvX"
                     /locus_tag="VHR42_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX3026501.1"
                     /translation="MKILAVDLGRARTGLAICDESELLASPAGVISEYNME"
BASE COUNT          626 a          630 c          639 g          630 t
ORIGIN      
        1 aatcgggagc ttcgctccgg tggcggactt cagacctccc ggctttgccg gacgaaggga
       61 gtatgaaacc attgggcagg cacccacctg ctttcgtagc aggttatttc agcacattac
      121 ggccaggcgg gattataaaa aagttgaggc ttctgattga aaaatcagaa gccttttttg
      181 tttctgtccg ggacaaacgg gccgtctcca tactgtcctc ctattaaaag gaaatttatg
      241 gggagacgag aatgaaccgc aaagatgtcc gctctttcgt ggaagggcat tgttaaagtc
      301 atttctgaca atttagaggt tttgtctata gacagtctcc gagtgatatt ctataataca
      361 gataaaacaa ttttccaatg tgttcaaaag gtggacgact cttacggtat cctaaggaat
      421 tgctatttcc cgtcaacgga aagccgctgc ctaaagtacg agtaatctac ttaaagttta
      481 tctgcttatc gaaagcaagt tcattgcata ccttacagga aacatcactc gaattatttt
      541 tgcgcctttc agtttgaagg cagaaaggaa cgagacttaa aatggtagac tttataattg
      601 ctgcagcttc gacggcagac cttccggcgg aatattttgc ggagcatgat gtgccggtga
      661 tccgttacac ctacataatg gacggcaagc cgtttgaaga cgattgcaaa gaagaaacaa
      721 gagaaaagat atataagcgc atgcgggaag gcgctgtaat gaccacctcc atgatcaata
      781 cctacaccta ttatgacttt tttaagcagc ttctggatac cgggaaggac gtcatctttc
      841 tcgatatgac gcgtcagatc tctcattcct attccaatgc cgagaaaacg gcggaacaga
      901 tcaaaacgga ataccccaaa cagcgttttt accttatgga taccctgtgc gtatcgggag
      961 gcctcgggct gcttctgcat tatatggtca acctgagaga cgcaggcaag agttttgacg
     1021 agacaatcaa atgggccgaa gccaacaagc ttaaaattat gcactggttt acggtgggcg
     1081 acctcaacta cctcaaacgg ggcggacgcg tttcgaacgc ggcggcaatg gttggttcgc
     1141 tcctttccat taagcctgtc ctctatgttt cggaagacgg aaaactcgtt gtagccaaca
     1201 agattcgcgg caggaaagca gccctggtgg agatacttga ccgcatgaaa aaggatttca
     1261 ccgaaccgga tggcaaagag gtattcataa accacgcgga ttgcctggag gatgcggaat
     1321 ttatgcggga ccagattctt gaaactttcc ctgccgtgtc gaaggtaacg attcggaacc
     1381 tcggcgtcgt gatcggcgcg cactgcgggc cgggactttt ggcgatattt tatctgggaa
     1441 acaagcgcta cgcatagaac cggaataaaa ttccgtagga agtccagcgg ataaataaac
     1501 actcaaaagg ggacgtgcac ccgggaatcc ggatgtacgt ccccttttct tatttacagc
     1561 ccgccattat ttttctgatt tctgcggtag tcgagataat cctgcagaat aatggtcgcc
     1621 gcgaccgcgt cgatcaccgc tttgcgcttc tttccgcgcg tatcggtcgt attcaggtat
     1681 ccgtgcgccg ttatggtggt gccgcgctca tcccgcatga ccaccggtac gcttaccagc
     1741 ttttgaaggt tctccgcaaa ggcgcgggca ttctgcgcgc tttccccctc gctgccgtcc
     1801 atattacgcg gtaggccgac cacgatgcac cccgcagctc tttctccggc ctgatcggcg
     1861 accgtctgcg caaggcgctc catgttgtat tcgctgataa ctcccgcggg ggaagccagc
     1921 agttcacttt catcgcatat ggcaaggccc gtgcgggccc ggcccaaatc cacggcaagt
     1981 attttcatct tattcctccg cagcgctatt cctgaggcag ccaccatgtc tggtgcataa
     2041 aaatcgaggt ttccggcgta agatgggaag cgtcgttcat catgaccaca tggctgtgca
     2101 gctttttgtc aaaatcgatc tgactgatgg cggtgttgtc gcaccagccg acctgattga
     2161 tctgttcaat gggcttattc agagcgtgac acaggaaatt gcggattgcg cagccgtggg
     2221 aggtcacgca tacggtcttt ccctgatttc cccttacaat atcggtcacg cattcccaca
     2281 tccggttata aacctcgcgc atggtttccc cgccgctcgg ggtaaaatca tacggtcttt
     2341 tgttccatac ttcaatttct tccgggtaaa tggaaggcag ttcatcccag gaataagcgc
     2401 tgcggaggaa tgcaatgaaa atacttgccg tagatttggg gcgggcccgt acgggcctcg
     2461 ccatatgcga tgaaagcgaa cttctggctt cccccgcggg agttatcagc gaatataaca
     2521 tggag
//
LOCUS       DATALY010000159         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_724612,
            whole genome shotgun sequence.
ACCESSION   DATALY010000159 DATALY010000000
VERSION     DATALY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724612"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VHR42_04670"
     CDS             <1..230
                     /locus_tag="VHR42_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HEX3026502.1"
                     /translation="DFEYEFQMALTNMSLSPETETIFLPASIDCMFLSSSMVREVARH
                     GRDVTKFVPEAIAKDIERKLYGHAEGSQDEE"
     gene            278..733
                     /locus_tag="VHR42_04675"
     CDS             278..733
                     /locus_tag="VHR42_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX3026503.1"
                     /translation="MSIDELLNVLDDMVDKAWGLPLTGGRCVLDAERVREIIDDIRLS
                     LPQEIRQAKAIVADRTEIIKNAKAESESIIRSAEEKARVLISQEELLKQAQTKANDLL
                     NETQTKSREMKKAAADFADNLMKNTEENLMQTLGEIKQARQALKTPNKL"
     gene            964..1383
                     /gene="mraZ"
                     /locus_tag="VHR42_04680"
     CDS             964..1383
                     /gene="mraZ"
                     /locus_tag="VHR42_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX3026504.1"
                     /translation="MLIGEYQHNIDAKGRVFIPAKLRDDLGETFIVTKGLDNCLFVYS
                     MQEWRNLESKIRELPMSKARNLQRFFFSGAAEVEVDKQGRILLPLNLREYAGLGKDVM
                     IVGSSIRAEIWDRSKWNEVCVGITSQSVAEAMDELEF"
     gene            1440..2375
                     /gene="rsmH"
                     /locus_tag="VHR42_04685"
     CDS             1440..2375
                     /gene="rsmH"
                     /locus_tag="VHR42_04685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX3026505.1"
                     /translation="MDREFSHRSVLLRESIEALNINPDGVYVDGTAGGGGHSFEIASR
                     LSGGRLIAIDRDPDAIRAAGEKLAPFGSRVTLVNDIFSRMKSVLNGMGIGAVDGILLD
                     LGVSSYQFDTPERGFSYHYDAPLDMRMSQQGRSARELVNQSSYHELVRILREFGEERF
                     APRIASLIVSNREKKPIETTFELIDLIKAAIPAAARRQGPHPARRTFQALRLAVNGEM
                     DELSMFLDDALSMLNPGGRLAVITFHSLEDRMVKHRLLSWAEGCICPPDFPVCVCGRK
                     PSAKILRKKGFEPSEQEIEENPRSRSARLRVAEKL"
BASE COUNT          698 a          525 c          694 g          553 t
ORIGIN      
        1 cggattttga atatgaattt cagatggcgc tcacaaacat gtcgctcagc ccggaaacgg
       61 aaactatttt tcttccggca agcattgact gtatgttctt aagttccagc atggttcgag
      121 aggtcgcccg gcacggaaga gatgtaacca agtttgttcc ggaagcaatt gcgaaggata
      181 tcgaacgtaa actttacggg cacgcagaag gcagccagga tgaagaataa cgttttatag
      241 aaaagatgcc gctcggcgcg gcggaaagga aaaataaatg agtatagacg agctgctgaa
      301 tgttcttgac gatatggttg acaaagcgtg ggggctgcct ttaaccggcg ggcgctgcgt
      361 gcttgacgcg gagagggtcc gggagattat cgacgatatc cgtttgagtc tgcctcagga
      421 aatcaggcag gccaaggcga ttgtggccga ccggacggaa atcatcaaaa acgcaaaagc
      481 agaatccgag agcatcattc ggagcgctga ggagaaagca agggtgctta tttctcagga
      541 agaactgctc aagcaggcgc agaccaaagc gaacgatttg cttaacgaga cccagacaaa
      601 atcaagggaa atgaaaaaag cggcggcgga ttttgccgat aatctgatga aaaacacgga
      661 agaaaatctg atgcagacgc ttggggaaat caaacaggcg cgtcaggcgc tgaaaacgcc
      721 caataaacta taacgtagaa atccaaataa gtttatcaaa gcggggcaaa ccacaacatg
      781 ttgtggtttg ccccgcttgc gtcactaacg gcgaaaaaaa gtcaatcggt taacataaaa
      841 acagtggacg taaatcttga attttcaaag ggaagccgtt ataatttaaa gcagagtggg
      901 tcaaagtgga gcaaagtgga gctaaaatat aaatcagatt atagatttgg tggagtaaaa
      961 gagatgctga ttggtgaata tcaacataat attgacgcca aaggccgggt ctttatccct
     1021 gcgaagctca gagatgacct cggtgagact tttattgtca ccaaagggct cgataactgc
     1081 ctttttgttt attccatgca ggagtggaga aatttagaat ccaaaattcg tgagctgccc
     1141 atgtccaagg cccggaattt gcagagattt ttcttctcgg gcgccgcaga agtggaagtg
     1201 gacaagcagg ggaggattct ccttcccctg aatctaaggg aatacgccgg tcttggcaaa
     1261 gacgtcatga ttgtcggttc ttccattcgc gcggaaatat gggacagaag caagtggaac
     1321 gaggtctgcg tcggtataac ctcccagtcg gttgcggaag ctatggatga attggaattt
     1381 tgaccgtcca aaggatggcc gaagagtgat ggacgcatga ttggcgtacg gaggttttta
     1441 tggatcggga attcagccat agaagtgttc tgctgcggga gagcattgaa gctttaaata
     1501 ttaatccgga cggggtttat gtcgatggga cggcgggagg cggaggtcac tcgtttgaaa
     1561 tcgcgtcaag actcagcggg gggcggctga tcgccattga ccgcgatccg gacgcaattc
     1621 gtgccgcagg cgaaaaactg gcgccgtttg gcagcagagt aacgcttgtc aatgatattt
     1681 tttccagaat gaaaagtgtt ctgaacggga tgggcatcgg ggctgtggat gggattctgc
     1741 ttgacctcgg cgtttcctcc tatcagtttg atacgcccga acggggcttt tcctatcatt
     1801 acgacgctcc gcttgatatg cggatgtcac agcagggcag gagcgcccgg gaactggtca
     1861 accagtcctc ctatcatgag ctcgtgagaa tcctccgcga attcggggaa gaacgatttg
     1921 ccccgcgaat cgcttcgctg attgtcagca atagagaaaa aaagccgatc gaaactactt
     1981 ttgaactgat cgacctgatc aaagccgcga tacccgcagc cgcccgaaga cagggcccgc
     2041 acccggccag aagaactttt caggcgctga gactcgccgt gaacggggaa atggacgagc
     2101 tttccatgtt tttggacgac gctctttcaa tgcttaatcc gggtggcagg ctggcggtga
     2161 tcacctttca ctctctggaa gacagaatgg taaagcatcg tcttttgagc tgggctgagg
     2221 gatgtatctg cccccctgat tttccggttt gtgtctgcgg ccgaaaacct tctgcaaaaa
     2281 tattgcgcaa aaaaggtttc gaaccatccg agcaggaaat agaagaaaac cccagaagca
     2341 ggagcgcaag gcttcgggta gccgaaaagc tgtaaattct gtcgaccggg atgtcgaaaa
     2401 tcaaagaatg ttgaggcgag gcaaatgaat tacggaaaag aaaatgtagc ctatgacttt
     2461 tcaagatttg
//
LOCUS       DATALY010000160         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_640599,
            whole genome shotgun sequence.
ACCESSION   DATALY010000160 DATALY010000000
VERSION     DATALY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640599"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VHR42_04690"
     CDS             <1..444
                     /locus_tag="VHR42_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3026506.1"
                     /translation="KAVVIIPAYHPTEQLCHMVQDLQASGFDKIIVVDDGSGKNSEPV
                     FQKIKAMGCDLLTHAANRGKGRALKTAFEHFLNFYAKDYFGVITVDADGQHIPADILR
                     MREALLTHPDTLVLGCRNFSGRDIPLRSRLGNRMFHFGVSFQISA"
     gene            686..1657
                     /locus_tag="VHR42_04695"
     CDS             686..1657
                     /locus_tag="VHR42_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HEX3026507.1"
                     /translation="MRLPFVGYKLQFELNARHHNIKNADAAPHPHTFTISLYLKKTGE
                     EFVLYTDIETDITDWLQQYQGKDLDGTELFSDRDETLETIGERFYSVLKERVRPKGYE
                     LVRLDIYENPVRTYSVSEKPVDGSINEHSAPISFLLGQAASEADPAQESAPGKETAQP
                     ALKSKPVLPVPPRIFAGEAPEENRERPKGRFPKLIAALLLLLLCSAGTVYLIKINGLY
                     PGGYDVMYHLYRSDSLFHSIVGGDWYPLYDRFFYNGVQPLRYWPPLPVYVMSLCRFLT
                     GNMLDSYLLYVGLIFFLGGCGWLTFGWKYNRIGISALVGVLWFFCAG"
     gene            1659..>2952
                     /locus_tag="VHR42_04700"
     CDS             1659..>2952
                     /locus_tag="VHR42_04700"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HEX3026508.1"
                     /translation="MMVLFGSGNLLRAMVAALLPYLLFFVWRFIDGKKWLSMIPVMLF
                     SSLMFLSHSGETGIVLICLLLFLLIHAAVSRRIREHGLLLLGILVSALIIGLWLYPSV
                     HGGLVSASGSDTQNLQSSFANGWTSLNPFARLSDKDSTYFGLSFFLLSIFGIFLGYKK
                     TFPGFLTGILIYFGTTLSLYPLISKLPMANLLWMTRFITAALAFVFLSFLLWKQLRKA
                     IVLFFCLLIALDSLPSVQYICTPAQARVSDVSAVLTRHAEETSLKDAKKITIQRIALM
                     DQSSYGSFAPWYLSGTKPETMCTFGAAWSSAQTAANIVQLNTAFSWGYYDYLFDRCLE
                     LGNDTVQIRIALLQHGAADIEKAVKAGKRLGYSLVGRTNSNLLFHKQISGAFGTKTVY
                     KGIAIGTSSRDIALLYPIFKETTDTDLNHYTFDQLKQYS"
BASE COUNT          684 a          724 c          726 g          818 t
ORIGIN      
        1 aaagcggttg ttattattcc cgcctatcat cctaccgaac agctttgcca tatggttcag
       61 gatcttcagg catccggatt tgataaaatc atcgtcgtag acgacggaag cggtaaaaat
      121 tcggagcccg tattccaaaa aataaaagcg atgggctgcg accttctcac ccacgcggca
      181 aaccggggga agggcagggc tttaaaaacc gcatttgagc attttttaaa tttctatgca
      241 aaagattatt tcggtgtaat caccgtagac gccgacgggc agcatattcc ggctgatatt
      301 ctgcgcatga gagaggcgct gctcacgcat ccggacacgc tggtgctcgg ctgccgcaat
      361 tttagcgggc gggatattcc cctgcgcagc agacttggaa accgtatgtt ccatttcggg
      421 gtctcctttc aaatttcagc ctaaatttta ccgtaatcca tgaaaaaatg ttgctgtaat
      481 ctataattta atgtataatc aaatagagta tatcgacacg gtttttccaa atcgtttaaa
      541 aaccgcaaaa ataccgcgtt cggtttatgt gtgaaacgct cgttttcagt ataaagtaaa
      601 tagctgcata attcaacaga aatcccaaat ttttgcggtt tgtacatttt ccttgagaaa
      661 attttgttat cggctgagag gacagatgcg tttgcctttt gtgggttata agcttcagtt
      721 tgaattaaat gcaaggcatc ataatattaa aaatgcggat gcggcccctc atccccatac
      781 ctttacgatt tctctttatt taaaaaagac gggcgaagaa tttgttttat acacggatat
      841 tgaaacagat ataacggatt ggctgcagca gtatcaggga aaggacttgg acggcaccga
      901 gcttttttca gaccgggacg agaccctgga aacaatcggc gaacggtttt attccgtgct
      961 gaaagaacgg gtacggccaa agggctacga actggttcgg ctggacattt atgaaaatcc
     1021 ggtacgtacc tattcggtgt ctgaaaaacc tgttgacggc agcataaacg aacattcggc
     1081 accgatttct tttcttttag gacaggctgc ttccgaagcc gatcccgcgc aggaaagtgc
     1141 gccgggaaaa gaaacggcgc agccggcgct gaaaagcaaa cctgttcttc cggttccgcc
     1201 gcgtattttt gcgggggagg cgcctgagga aaacagggaa cgcccgaagg gaagatttcc
     1261 aaaacttatc gccgcgctcc ttcttcttct gctgtgttcc gccgggacag tttaccttat
     1321 taagatcaac ggcctttacc cgggcggcta tgacgtaatg tatcacctgt accgttccga
     1381 ttcgctgttt cactccattg tgggcggaga ctggtatccg ctttatgacc gcttttttta
     1441 taacggcgta cagcctttgc ggtattggcc gccgcttccc gtatatgtga tgagcctttg
     1501 ccggttcctt acggggaata tgctggattc ctaccttctg tatgtagggc tgatattttt
     1561 ccttggcggc tgcggctggc ttacgttcgg ctggaaatac aaccgaatcg gtatttcagc
     1621 gctggtcggc gtgctgtggt ttttttgcgc cggataacat gatggtgctt ttcggaagcg
     1681 gaaacctgct gcgcgcaatg gttgcggcgc ttctcccgta tttgctgttt tttgtctggc
     1741 ggtttatcga cggaaagaaa tggctcagca tgatccccgt tatgctgttt tcttccctga
     1801 tgtttctcag ccattcgggc gagacgggga ttgtactgat ctgtcttctt ctgtttttgc
     1861 tgattcacgc ggcggtcagc cggcgaatca gggaacacgg ccttttgctt ctcggtatcc
     1921 tcgtatccgc cctgataatt ggattatggc tgtacccgtc cgtgcacggc ggtctggttt
     1981 ccgcgagcgg cagcgatacg cagaatcttc aaagctcctt tgcaaacggc tggacgtccc
     2041 tgaacccgtt tgcaagactc agcgacaagg attccactta ttttgggctt tcgttcttcc
     2101 ttctgagtat ctttggaatt ttccttggat ataaaaaaac gtttccgggc tttttaaccg
     2161 gtatcctcat ctatttcggt acgaccctat cactttatcc gctgatttca aagctgccga
     2221 tggcaaacct tctctggatg acccggttta tcacggcggc gctggcgttt gttttcctgt
     2281 cgttcctgct ttggaaacag ctcaggaaag cgattgtcct ctttttctgc ctgcttattg
     2341 cgttggattc tctgccttcc gttcaataca tctgcacgcc tgcgcaagcc cgggtttcag
     2401 acgtgtcagc ggttctgaca cggcatgcgg aagagacttc cctgaaagac gcaaagaaaa
     2461 taaccattca gcgtatcgcg ctgatggatc agagcagcta cggttctttt gccccgtggt
     2521 atctctccgg aacaaagccg gaaaccatgt gcaccttcgg cgcggcgtgg tccagcgctc
     2581 agacggccgc caatattgtg cagctgaaca ccgctttttc ctgggggtat tacgattatc
     2641 tgtttgaccg ctgccttgaa ttgggaaacg atacggttca gattcgcata gcactcctgc
     2701 agcatggcgc cgcggatatc gaaaaagccg tgaaagcggg gaagcggctc ggctacagct
     2761 tagtcggaag gacaaattcc aaccttctgt ttcacaaaca gatcagcggc gcattcggta
     2821 ccaaaaccgt ttacaaaggc atcgcaatcg ggacttcctc gcgtgacatc gcgctgcttt
     2881 acccgatctt taaagaaaca accgataccg acctgaacca ttacaccttt gatcagttaa
     2941 agcagtattc ca
//
LOCUS       DATALY010000161         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_121903,
            whole genome shotgun sequence.
ACCESSION   DATALY010000161 DATALY010000000
VERSION     DATALY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121903"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1134
                     /locus_tag="VHR42_04705"
     CDS             133..1134
                     /locus_tag="VHR42_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX3026509.1"
                     /translation="MMKNKAVKKGKKKKILLIILSVCLGILLISGASMYFYLNGMLGK
                     INYKPITSKTSSSSGTSGTGDTLVSSKDVYNILLVGSDTRTTSTSNSRSDSMILLSIN
                     NKTKKTIMTSFMRDIYIPIPGHGSNRLNAAYSLGGISLLEQTIQNNFNVKIDRYVSVD
                     FYDFINIIDKLGGINISVKDDEVKVLNEYINEINKLKGLPDGNGFLKKGGDDLLLSGK
                     QALAYSRIRYVGNADFERTERQRTVLTKVIAKLKKQNIVQLSSVLSMLLPDITTDFTR
                     NELMNVVMHALTYTGYSSEQYRVPIDGSYHGIYIDKMDVLSIDFDQNKQALKTKING"
     gene            complement(1213..1686)
                     /gene="ybaK"
                     /locus_tag="VHR42_04710"
     CDS             complement(1213..1686)
                     /gene="ybaK"
                     /locus_tag="VHR42_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX3026510.1"
                     /translation="MNKTNVMRLLDSAKISYHTASYEYSEDDLSGVHAAEQISEICPE
                     QCFKTLVARGEKQGILVFCVPVADELDLKRAASAAGDKKVELIAVRELQGITGYIRGG
                     CSPVGMKKKFPVYLDSSAKNYDLIGVSAGTRGLQIILSPLDLADYTGAKFCTLKR"
     gene            complement(1722..>2881)
                     /locus_tag="VHR42_04715"
     CDS             complement(1722..>2881)
                     /locus_tag="VHR42_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3026511.1"
                     /translation="IGPGAGIHGGNVICSGTIEDVKNCKESITGQYLTGAKRIEVPAH
                     RRKGNGNSLVIKGARENNLKNLNVKIPLGTFTCVTGVSGSGKSSLVNEILYKKLAAEL
                     NGAKTRAGEHDSVEGLEYLDKVIDIDQSPIGRTPRSNPATYTGVFTDIRNIFASTQEA
                     KMRGYSSGRFSFNVKGGRCEACQGDGIIKIEMHFLPDIYVPCDVCKGKRYNRETLEVK
                     YKGRSINDVLEMTVEEGMNFFESVPRIYRKLKTLFEVGLGYVKIGQPATTLSGGEAQR
                     VKLATELSKRPTGKTIYILDEPTTGLHTADVHRLIEVLQKLVDTGNTVVVIEHNLDVV
                     KTADYLIDLGPEGGDRGGNIVVCGTPEKVVKCESSYTGKYLKKYLPPKSKG"
BASE COUNT          735 a          699 c          656 g          791 t
ORIGIN      
        1 gacagcgggc atccgtcttt tacgggttga cattaattcc tttaagtaat atagttatta
       61 tgataaattt tgaaaaatcg tgtcgaaaat tacagattgc gataaagatt taattggaaa
      121 agagtgttga gtatgatgaa aaataaggcc gtaaaaaagg gcaaaaaaaa gaaaatttta
      181 ttgataattc tgtccgtctg tcttggaatt cttctgattt ccggggcatc aatgtatttt
      241 tacttaaacg ggatgctggg taaaatcaat tacaaaccga tcacttccaa gacatcgagc
      301 agcagcggta ccagcggcac aggcgatacc ctggtatcaa gcaaagatgt ttataacatt
      361 ctattggtag gctccgacac caggaccaca agtacctcaa acagccggtc agacagcatg
      421 attcttcttt caatcaacaa caaaaccaaa aagacgatta tgacttcctt tatgcgtgac
      481 atctacattc ccattcccgg ccatggaagc aaccggctta atgcggcgta ttccctaggc
      541 ggaatcagcc ttttggaaca gacaatccag aataatttca atgtaaaaat tgaccgctat
      601 gtttccgttg atttttatga ctttattaat attatcgaca agctcggcgg aatcaatatt
      661 tcggtaaaag acgacgaggt caaggttctc aatgagtata taaacgaaat caacaaatta
      721 aaagggcttc cggacggaaa tggattttta aaaaaaggcg gagacgacct gctgttatcc
      781 ggcaaacagg cgcttgctta ttccagaata cgctacgtcg gaaacgcgga ctttgagcgt
      841 accgaacgcc agaggaccgt tttgaccaaa gtaattgcga aactgaaaaa acagaatatc
      901 gttcagttgt catccgtgct tagcatgctg ctgccggata tcacaaccga tttcaccaga
      961 aatgaactta tgaatgttgt catgcatgcg ctgacctata ccggttattc gtcagaacag
     1021 tatcgcgttc cgattgacgg ctcttatcat ggcatctata tcgacaaaat ggatgttttg
     1081 agcattgact ttgatcagaa caagcaagca ctgaaaacaa aaattaacgg ttaaaccgca
     1141 cttgcgaaaa gccacggcct caaatcggcc gtggcttttt tgtttgtctt ttttgtttga
     1201 taaaatcgcg cttcatcttt taagagtgca aaactttgcc cccgtgtaat ctgcaagatc
     1261 cagcgggctg agtataatct gaaggcccct cgttcccgcg ctgacgccga tcaggtcata
     1321 gttttttgca gacgagtcca gataaaccgg aaatttcttc ttcatgccga ccggggaaca
     1381 tcctccccgg atatagccgg ttataccctg cagctcccga accgcaatta gctctacctt
     1441 tttgtcgccg gccgccgacg cagcgcgctt tagatccagc tcgtcggcaa cgggaacaca
     1501 aaaaacaagg atgccctgtt tttcacctct cgccaccagg gttttaaagc actgttccgg
     1561 gcaaatctcg gaaatctgtt ccgcggcgtg aacgccgctc aggtcgtcct cgctgtattc
     1621 gtagcttgcg gtatgataac tgatttttgc cgagtcaagc agccgcatga cgtttgtttt
     1681 attcataatc tgtttcacct gtttgttctg tcggcccgcc tttaaccttt tgatttgggc
     1741 ggaagatatt tttttagata tttgccggtg taagagcttt cgcatttgac cactttttcc
     1801 ggtgtgccgc agacaacgat gttcccgcct ctgtcccctc cctcagggcc aaggtcgatc
     1861 agatagtcgg ccgttttaac aacatccaga ttatgctcga tgacaacaac cgtattgccg
     1921 gtgtcgacca gcttctgaag aacctcgatc agcctatgga cgtcggcggt atgaagaccg
     1981 gttgtcggtt catccagaat atagatggtt tttccagttg gccgcttgga aagttcagtg
     2041 gcaagcttta ctctttgggc ctcgccaccg gaaagcgtgg tcgccggctg accaatttta
     2101 acataaccca gcccgacctc aaagagagtc tttaatttcc ggtaaattcg gggaacgctc
     2161 tcaaagaaat tcatgccctc ttccacggtc atttccagca cgtcgttgat actccggccc
     2221 ttgtatttga cttcgagggt ttcacggttg taacgcttgc ctttgcagac gtcgcaggga
     2281 acgtagatat ccggcagaaa atgcatttca atcttgataa taccgtctcc ctgacaggct
     2341 tcgcaccgtc cgcctttgac gttgaaagaa aacctgcccg aagaatatcc gcgcattttt
     2401 gcctcctggg tagacgcgaa aatattacgg atgtcggtaa aaacgccggt ataggtggcc
     2461 gggttggaac ggggcgttct gccaatcggc gactggtcaa tatcaatgac cttatcaaga
     2521 tattcgagac cttcgaccga gtcgtgttct ccggctctgg ttttcgctcc gttaagctcg
     2581 gccgccagct ttttatagag tatctcgttg accagcgagc tttttccgga acccgatacg
     2641 ccggtcacgc aggtgaacgt gcccagtgga attttaacat tcagattctt gaggttgttt
     2701 tctctcgcgc cttttatgac aaggctgttt ccgttgccct tccgtcggtg agcgggaact
     2761 tcaatgcgtt ttgccccggt cagatactgg ccggtgattg actccttgca gttcttcaca
     2821 tcttcgattg ttcccgaaca gatgacgttt ccgccgtgga ttcccgcgcc ggggccgata
     2881 a
//
LOCUS       DATALY010000162        11837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_811489,
            whole genome shotgun sequence.
ACCESSION   DATALY010000162 DATALY010000000
VERSION     DATALY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811489"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..356
                     /locus_tag="VHR42_04720"
                     /pseudo
     CDS             147..356
                     /locus_tag="VHR42_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
     gene            complement(357..1088)
                     /locus_tag="VHR42_04725"
                     /pseudo
     CDS             complement(357..1088)
                     /locus_tag="VHR42_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
     gene            complement(1208..1801)
                     /locus_tag="VHR42_04730"
     CDS             complement(1208..1801)
                     /locus_tag="VHR42_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3026512.1"
                     /translation="MLIACMMVLDRTGLAVFALLCAAFHETGHLTALKIFKIDVEEIS
                     VRAFGVSIKLRNDSRVSYRQEIVVSLAGAAANLIFALAAFVLQNLGVLPYSMNVLWVF
                     NLIIACFNLLPIGPLDGGRCLECILLTRMHYSGAQIIVNVVSFLIIIPLTIAGYFLVV
                     STGYNFSLIMAAVYLAVLLLLKGRLIIGGSYPKPRLR"
     gene            complement(1858..2838)
                     /locus_tag="VHR42_04735"
     CDS             complement(1858..2838)
                     /locus_tag="VHR42_04735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3026513.1"
                     /translation="MYDDEERNTTYQRRYRSRSKSEPRRFPVSFTGPQVFICAIVLLA
                     ALIFKLTGGSVYIKFKSQATDIFNKSVTNSQLQQVFSNIKKQFPNAAEVFKNSDSQSN
                     KTSSINSAASSGASGSGTASTGSVSAGAGKTSSKASSNTSSAKSSQTSSKASSGVSTI
                     TIGENLNGQGGEDLEATRSPASGLLVPPKTASLGPFRLSFQLIRPVSGKVTSGFGYRI
                     NPITKKLSFHTGIDLASPEATPIKAALPGKVAEVGKSVAYGNYILLEHGGGIQTFYGH
                     CEKILKKQGDDITAGAAIATVGSTGWSTGYHVHFEIRINGVRYNPLWLIK"
     gene            3028..4170
                     /gene="wecB"
                     /locus_tag="VHR42_04740"
     CDS             3028..4170
                     /gene="wecB"
                     /locus_tag="VHR42_04740"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX3026514.1"
                     /translation="MNKIKVLMIFGTRPEAVKMAPLQKALSESDLFDCRVCVTAQHRG
                     MLDQVLDIFKIVPDYDLDIMKSAQTLSYITTGVLNGVDQVIKTFRPDIALVHGDTTTS
                     FGSALAAFYNQIPVGHVEAGLRSYDIYSPFPEEINRRLTGQIAAVHFAPTPANRENLL
                     RENIKDHIYVTGNTVIDAMKTTVKKDYRFESETLRKISGHPGKIILMTAHRRENIGEP
                     LKNICRAVKKVAQDNKDVKIVYPVHPNPAVLNTAREILGNLENVTLCEPLNINDMHNI
                     MARSYLVLTDSGGLQEESPALGVPVLVLRTETERPEAVEAGTVKMAGTEENTIYTMAQ
                     TLLRDRSEYEKMARAVNPYGDGRACERIRRAILHYFGKGDSPEEFR"
     gene            complement(4395..5084)
                     /locus_tag="VHR42_04745"
     CDS             complement(4395..5084)
                     /locus_tag="VHR42_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX3026515.1"
                     /translation="MILSGVFVNTIAIALGGLFGTFFKKGVSQRLNRSLMQGLALCVL
                     YIGVRGTLQGKNIVIAILSVIIGAILGEIFDFNQKLQSFGDYIQGKLPAQKTGSSIAE
                     SFVTSTLFVCVGAMAIVGSLQSGLTGSHETLYAKALIDGIVAIVMASTMGIGVSLSAV
                     SVLVYESVLTLSAHAISSCLTTTVINEMTCVGSLLIIAIALNMLKLTNIKVANFVIAP
                     FIPIGLCMILR"
     gene            5194..6102
                     /locus_tag="VHR42_04750"
     CDS             5194..6102
                     /locus_tag="VHR42_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3026516.1"
                     /translation="MKGGSGISIEKYQAFIKTVELGSLSKAAEALGYTQSNITHMLQG
                     LEREYGFQLLIRNKAGIFLTENGKQVLEPMKKVISWNENLSQTVAKINGLQTGTLRIG
                     MFPSVAVEWLPDIIQKFQKKYPGIQIRLMDGDYDEIAEWLKSGKIDCGFLAQSENMAV
                     NFIPLIQDAMLAILSKEHPMSNAATFPVGKFSEEAFIFPHRGLDSDVRQVLQQAKVIP
                     NIKYDVRGDDAIIALVNKNLGVGMLPELYLKNHSKGIITKRIDPNFYRIIGIAVPEQE
                     YKLPVVTQFIDCLQKWLKDHYDDVLF"
     gene            complement(6205..6723)
                     /locus_tag="VHR42_04755"
     CDS             complement(6205..6723)
                     /locus_tag="VHR42_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026517.1"
                     /translation="MSKTAKITMGGMLAALSVALLFLSNFISVASLALSAIAGVLLIP
                     AVIEASAGWAFMIYGVVSILSLLLLGDKSSALFYIFLFGHYPIVKNAIERKLQNKPLQ
                     WVIKLLFQNVCAFIAMLITYKVTGVGDDSLFKNGLMVTAILFNVTFVLYDIALSRLIV
                     TYVYRIRKIFHH"
     gene            complement(6743..7378)
                     /locus_tag="VHR42_04760"
     CDS             complement(6743..7378)
                     /locus_tag="VHR42_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX3026518.1"
                     /translation="MNDPYKILGVSPQATDDEVKAAYRELAKKYHPDNYVDNPLSDLA
                     TEKMKEINEAYDEVVRQRRSGSGSSRSSGGSYQGNSSGYQSSGFRNIRDMISQGRTAE
                     AQAALEAVAYKDRGAEWYFLKGSLMYNKGWVNEAYANYQKAVQMDPNNPEYRQAYETV
                     NDQMNGGFYGRNRDFGGYNTGRQGGCSGCDLCTSLLCADMCCECGGGNLCC"
     gene            complement(7418..8347)
                     /locus_tag="VHR42_04765"
     CDS             complement(7418..8347)
                     /locus_tag="VHR42_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3026519.1"
                     /translation="MKNLKAAYLVRILIFGLIFVFTLAGCSSGSKKTESAASSKLASA
                     ANTSSVKSSSVSESSSSKEPVSSSSLPKISGTGVKVLMYHDIMTAQQKGKVKNGLVIT
                     TEEFDRQMAILKSAGFETITPDELYDYIKGKTNLERKILITFDDGYKTCGCLAAPILK
                     KYGFRATIFVITGYLDPTDQPANTSTFAIQYLSTNDLSRYSYALDYGSHTEKMHDEYR
                     SKPMLISKSSSEILADLSKSRQKLNGTDSIAYPFGKYSDKTIQLVKQAGFKMAFTTKP
                     KTVQPGIDPYEIPRFEVNAPMTDYHFKTIVGIR"
     gene            complement(8370..9278)
                     /locus_tag="VHR42_04770"
     CDS             complement(8370..9278)
                     /locus_tag="VHR42_04770"
                     /inference="COORDINATES: protein motif:HMM:NF039826.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HEX3026520.1"
                     /translation="MFGYIRPFKPEMKFKDYDSFRAVYCGVCHSLGKRYGFINRMLLS
                     YDAAFLAALRFAAGSGCGGFQKKRCPYQPLVKCLCCVESDELNFAADVAAILYYYKVK
                     DNLRDARGLRKAAAALLLPFASATHRRAVKFRPEAESVVSRYLESQRQIEKKQSISID
                     EAACPTGEMMSSIASCGVQDGAEKRILERLGYFLGRWIYLMDAADDLEKDLADNSYNP
                     FVCAFQLQKGEKAARSTQEIERSLNPSIAEITAAYELLSPAGFDPVLRNIVYLGLPGM
                     QRKVLKKLNSCGKSKNAAHPMTVEEN"
     gene            complement(9431..10636)
                     /locus_tag="VHR42_04775"
     CDS             complement(9431..10636)
                     /locus_tag="VHR42_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HEX3026521.1"
                     /translation="MGVIKIKEGIYSVGVLNPNMRIFDVIMRTDYGTSYNSYLIKSEK
                     NVLIETVHPRYFDEYYENIASVIDPKTVDYVIMNHCEPDHSGSLARLFEMAPQITVIT
                     SPAGAIYLKNITNKPDLKIKVVKDGDTLDIGGGKVLRFLSAPFLHWPDSMFTWLESDK
                     IAFTCDFLGSHYCEPRMADSHVVYFDRFEKAFHEYFNAIFGPFKSYVLKGLEKLNTLD
                     ADYVCTSHGPVLTRGKFLETAKQRYGEWSRPVIRERPFIPVFFCSAYGNTEALAKEIV
                     RGVRSVMTDAEVKIYDINENDFADLEVKINQSDAFLIGSPTINKDAVLPVWKLCAGID
                     AINAKGRHCGVFGSFGWSGEAVPNIVSRLRSLNMDVFEGGYTCRFVPSEEEMNGAFEY
                     GKGFAEHLK"
     gene            complement(10830..10988)
                     /locus_tag="VHR42_04780"
     CDS             complement(10830..10988)
                     /locus_tag="VHR42_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX3026522.1"
                     /translation="MKKYVCSVCGYVYDEEKGCPDQGIAPGTKWEDVPDDFVCPMCGV
                     GKDMFEAQ"
     gene            complement(11304..>11837)
                     /locus_tag="VHR42_04785"
     CDS             complement(11304..>11837)
                     /locus_tag="VHR42_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HEX3026523.1"
                     /translation="ELFYKVLGETTGLEINGEGDLIETMMGLSKMKKEYDKIKDALDE
                     VNATGYGIVMPSVDELKLEEPEIVKQGGRFGVRLKASSESIHMLKCRVVTEINPIVGN
                     EKQSEELVTYMLKEFEENPEKIWESNIFGKSLHDLVTEGLHNKLYRMPADAREKLQET
                     LERIINEGCGGLICILL"
BASE COUNT         3212 a         2936 c         2666 g         3023 t
ORIGIN      
        1 tcaggtcgag gtgggaaata tatttcgccc ggccggtctt tcggaatcgg attcttaatt
       61 tctgcaaacc gggcaccccc cttcggcgaa cgacgcggcc ccgcagccaa ggcatttttc
      121 cctgcagttt tccgagactt cgctccgaca ggctttatta cccgggaaat ttcgttgttc
      181 cgtttatgga tattgtccat gaccggtcca tggtggaatt gtttcggggc tgcatccggg
      241 gctgccgttt ctgtcaggcg ggctttttat accggcccgt ccgtgagaaa agcccggagg
      301 tgatcaaccg tcagggcgaa gccttgtgcg atgcgaccgg ttacgatgaa atttcgcggg
      361 taataaagct tgtccaagtc gttaataatc cgcttagtta cttttttagg cactttttca
      421 gataccggcg tgacatcatg aatcgttccg tcactctggt aatcaacccg gtaaagcgac
      481 ggcacataga cgccctcaat ggacgaagca gccagcagga attcgcttct cgggcgattt
      541 tcctgtttaa accgtttgta aaggtcgatc acttcattga tgatttcttc gccctcgccc
      601 agacaaaaca ggtcgataaa atctgccagc ggttccggat tgcatgcgca gggtccaccg
      661 gccataacga tcggggaaag gccttccctg tcggcggaac ggatgggtat cccggcaaga
      721 tcgagcatgt tcaggatatt cgagaaactc agttcatact gaagcgtaaa accgatcaga
      781 tcaaaatcgc agatcgggtc gccgctctca agcccgaaaa gtttgatttg gttaagtctc
      841 atctgctctt caaaatcaat ccacggcgcg aatacccgct cacaccagac gtcttcccgg
      901 ttatttaatt cgctgtaaag gatcttcatg ccgaggtgag acattcccac ctcgtagaca
      961 tcaggaaaac aaaaggcata ccggatgtct acggcatttt tatctttcat aatactgttg
     1021 agctcgccgc cggcgtatct gcccggcttc tgaacttttc ttaagacaat ttccaattcc
     1081 ctgttcaaaa catttcctcc gatttaaaac catctttggc ttttaaaacg caaacttctt
     1141 ttttctgaac tatttttcgc agtaactata ataataaacg aaaaacggtt taatttcaat
     1201 catccattta ccgaagacgg ggtttcggat aggaacctcc gataatcagc ctgcctttga
     1261 ggagcaaaag cacggccaga taaacggccg ccataatcag ggaaaagttg tatccggtgg
     1321 atactaccag aaaataaccc gcgatggtca gcggtataat gatcagaaaa gaaacgacgt
     1381 tgacaataat ctgcgctccc gaataatgca ttcgggtaag cagaatgcat tccaggcatc
     1441 ggcccccgtc cagaggcccg atgggaagaa gattgaagca tgcgataatc aggttgaata
     1501 cccagagtac gttcatggaa tacggtaaga cgccgagatt ttgcagaacg aacgccgcaa
     1561 gggcgaaaat caggtttgcc gcagccccgg caagtgacac gacaatctcc tgccggtagc
     1621 tgacgcggct gtcgttcctc agctttatac tgactccgaa tgccctgacg gagatttcct
     1681 ccacgtcaat tttaaaaatc ttcagggcag tcaaatgacc ggtctcgtga aacgccgcgc
     1741 agagcagcgc aaaaacagcg aggccggttc ggtcaagaac catcatacag gcgatcagca
     1801 cggcaaaata aatgctgata ttaacacggt acggaataaa atcgcctccc gaaagaccta
     1861 tttaatgagc cagagcggat tgtatctcac accgttgatc ctgatctcaa agtgaacatg
     1921 ataccctgtg gaccatcccg tactgccgac ggtggcaatt gcggcgccgg ccgttatatc
     1981 gtcgccctgc tttttgagaa ttttctcaca gtggccgtaa aaggtctgga ttccgcctcc
     2041 gtgctcgagc agaatatagt ttccgtaagc gacacttttt cccacttccg caaccttgcc
     2101 cggaagagcg gccttaatcg gggtcgcttc aggagaagcc aaatcgatac ctgtatgaaa
     2161 actcagtttt ttggtaattg gattgatccg gtatccaaag ccggaagtta cttttcccga
     2221 aaccggacga atcagctgga aacttagccg gaatgggccc aatgacgcgg ttttcggagg
     2281 aaccagcaga ccggaagccg gggatcgggt agcttcaaga tcctcccctc cctggccatt
     2341 gagattttcc ccaatggtga tggtgctcac accggaagaa gcttttgagg aggtctggct
     2401 ggattttgcc gaggatgtat tggaagaggc ttttgaggag gttttgccgg cgccggcaga
     2461 caccgagcct gttgacgcgg tgcctgaccc tgatgcgcct gaagacgcgg cgctgttaat
     2521 ggaagatgtt ttgttgcttt gcgagtcgct gtttttaaaa acttccgccg cattcggaaa
     2581 ctgcttttta atattgctga aaacctgctg caactgactg ttggtaacgc tcttgttaaa
     2641 aatgtcggta gcctggcttt tgaactttat gtaaacggag ccgccggtca gcttgaaaat
     2701 cagcgccgca agaagaacga ttgcgcagat aaaaacctga ggacctgtga aactgaccgg
     2761 aaaacgacga ggctcggatt tggaacgcga tcggtaccgc cgctgatagg tagtgtttct
     2821 ttcttcatca tcatacattt ttaaaccctc ccaagctaat tatatgcggg agtggacagg
     2881 gttatcattt aaattttatt tttgccaaag aaagattccg gtttcgaaca tctttaccgg
     2941 gtttgaatac gccgcgtcat tttggtataa taggaagaga tttctttaaa aacatcaacg
     3001 gcaaccggcc gttatctgga ggtttctatg aataaaataa aagtgctgat gattttcggg
     3061 acaaggccgg aagcggtcaa aatggccccg ctgcagaaag ccctctcgga atcggatctg
     3121 tttgactgca gggtctgtgt caccgctcag caccgtggga tgctcgatca ggttctggat
     3181 attttcaaaa ttgttccgga ctatgacctt gatattatga agtctgccca gacgctctcc
     3241 tacatcacaa ccggcgtgct caacggagtg gatcaagtga tcaaaacctt ccggcccgac
     3301 atcgcccttg ttcacggcga tacgacaacc tccttcggtt cggcgctcgc cgcattttac
     3361 aaccagattc cggtaggcca tgtggaggca ggtctgagat cctacgacat ttattctcct
     3421 tttccggagg agataaaccg ccgcctgacc ggccagatcg cggccgttca cttcgcccct
     3481 accccggcga accgggaaaa cctgcttcgt gaaaacatca aagaccacat ttatgtgaca
     3541 ggcaacacgg tgattgacgc gatgaaaacc acggtaaaga aggattatcg ctttgaatcc
     3601 gaaacgcttc ggaaaatcag cggacatccg ggaaaaatca ttctgatgac cgcccacaga
     3661 cgcgaaaata ttggtgaacc gcttaagaac atctgccggg cggtcaaaaa agttgcgcag
     3721 gataacaaag acgttaaaat tgtttacccg gttcatccga atcccgctgt tttgaatacc
     3781 gcaagggaaa ttctcggaaa ccttgaaaac gttaccctct gtgagccgct gaacatcaac
     3841 gacatgcaca acatcatggc gcgctcctac cttgttttga cggacagcgg aggacttcag
     3901 gaggaatctc ccgcccttgg ggttcccgtg ttggtgctgc gcaccgaaac ggaacggccc
     3961 gaggcggtgg aagcgggaac ggtcaaaatg gccggaaccg aagaaaatac aatttacacg
     4021 atggcccaga ccctcctccg cgaccggtcg gaatatgaaa aaatggcccg cgcggttaac
     4081 ccatacgggg acggacgcgc ctgcgagcga atccggcggg caatcctcca ttatttcgga
     4141 aagggcgaca gtccggagga attccggtaa aaagaatccc tccctgcgtg gaacgcaata
     4201 gagaagttta tactttcatt cggattggca caggatatag gagtatcctg tgctttttcc
     4261 gttttgcgtg gatcgttacg gcaagacggg caagtaaagt aaaagttcta tatgtaaccg
     4321 gagcctgcga agctgcagaa aaaaatacaa agttttaatt agatgctctt taatattata
     4381 aactcaaaaa actactatcg taaaatcatg cacaaaccga ttggtatgaa tggagcgatc
     4441 acaaaattcg caactttaat atttgttaat tttaacatat tcagtgcaat cgcaataatc
     4501 aaaagggaac cgacacatgt catctcgtta ataacagtcg ttgttaagca actggagatc
     4561 gcatgggccg acaacgtcaa aacggattca taaaccagca ccgaaacggc agataaacta
     4621 accccgatcc ccatcgttga agccatcaca attgcaacaa taccgtctat cagcgctttt
     4681 gcatacagag tttcatgact gcctgtcaag ccgctttgaa gcgagcccac gattgccatc
     4741 gctccaacgc agacaaagag cgtgctggtt acaaagcttt ctgcaatcga gcttcctgtt
     4801 ttttgggccg gtaatttacc ttggatataa tcaccgaaac tttgaagctt ttggttaaaa
     4861 tcaaaaattt cacccagaat tgcaccgatt atgaccgata aaattgcaat gacaatattc
     4921 ttaccctgta aagtccctct gactcctata tataagacac aaagcgccag cccctgcatc
     4981 agcgaacggt tgagacgctg ggagactcct tttttgaaaa aagtcccgaa taatccgccc
     5041 agagcgatgg cgattgtatt gacgaataca ccagacaata tcatttttca atcccctttt
     5101 tcattatacc gtaaaaagta tatccgaaaa aatagtatta gtaaaatgaa taaatatcat
     5161 atataatatt ataaatcatg atattatata gaattgaaag gagggagcgg catcagtatt
     5221 gaaaaatatc aggcgttcat taagaccgta gaattgggaa gtctgtcgaa ggcggcggaa
     5281 gcattgggtt atacacaatc caacatcacg catatgctgc agggtttgga aagagaatat
     5341 ggatttcagc tgctaatccg taataaagcc ggcattttct tgacggaaaa cggaaaacag
     5401 gtattggaac ctatgaaaaa agtgataagc tggaatgaaa atctatcaca gactgtggca
     5461 aaaataaacg ggcttcaaac aggtacgctt agaattggaa tgttcccaag tgttgcggtg
     5521 gaatggctcc cggatatcat tcaaaaattc caaaaaaaat atcccggaat tcagatcagg
     5581 ttaatggacg gagattatga cgaaattgcc gaatggctga aaagcggtaa aattgattgc
     5641 ggatttttag cacaatccga aaatatggcc gtaaatttta ttccgctaat tcaagatgcc
     5701 atgctggcca tcttatcaaa agaacatccc atgagtaatg ccgccacttt tccagtcgga
     5761 aaattttcag aagaagcctt tatctttccg catcggggat tggacagtga cgtacggcag
     5821 gtattgcagc aggctaaagt gataccgaat attaaatatg acgtgagggg tgacgatgcg
     5881 attattgctt tggtgaacaa aaatctggga gtcggcatgc tgccggagct ttacctgaaa
     5941 aatcattcca aaggtattat aacaaaaaga atagatccaa atttttatcg tattatcggc
     6001 atcgctgtcc ccgagcagga atataaactt ccagtggtaa cgcagtttat tgactgcctc
     6061 cagaaatggc tgaaagatca ctatgatgat gttctgtttt aatgctgttt tttgttaata
     6121 cggccgttat ccttttgaaa aagcaaaact ccctgcaagt aaaaattcta cttgcaggga
     6181 gtttgttcac gcgctgcggg gctcttaatg atgaaaaatt ttacgtatcc ggtaaacata
     6241 agtaacaatc aaccttgaaa gcgcgatatc ataaagcaca aaggtcacgt taaacagaat
     6301 agctgttacc atcaagccat ttttaaataa gctgtcgtcg ccgaccccgg tcactttata
     6361 ggtaatgagc atggcgatga atgcacagac gttctgaaac agtagtttta tcacccactg
     6421 aagcggctta ttttgaagct tacgctcaat ggcgtttttg acgatcgggt aatggccaaa
     6481 cagaaaaatg taaaacaatg ccgaagactt gtcgcccaaa agcagcaaag aaaggatgct
     6541 gacgacgcca tagatcataa acgcccaccc tgcggaagct tcgatcaccg cgggaatgag
     6601 cagcacaccg gcaatcgcag acagcgcaag agacgccacc gaaataaaat tcgacaggaa
     6661 cagcagcgcc acagaaagcg cggcgagcat tccgcccatt gtgattttag ctgtcttact
     6721 cattcggttt catcactcca tatcagcagc aaaggtttcc gccgccgcat tcgcagcaca
     6781 tatcggcgca aagaagcgaa gtgcaaagat cacagcctga gcatccgcct tgtctgccgg
     6841 tattataacc gccgaaatcc cggtttctgc cgtaaaaacc accgttcatc tggtcattga
     6901 ccgtttcgta ggcctggcgg tactcggggt tattcggatc catctggaca gctttctggt
     6961 aattggcata agcctcattt acccatccct tgttatacat caggctgccc ttgaggaaat
     7021 accattcggc tcccctgtct ttataggcaa ctgcttcaag ggccgcctga gcctcggccg
     7081 ttcgaccctg gctgatcata tcccggatat tccggaagcc gctggactgg taaccgctgg
     7141 aatttccctg ataagagccg cccgacgaac gggacgagcc cgacccggat ctcctctgtc
     7201 ttacaacctc atcatatgcc tcattgattt ccttcatttt ttcagttgcg agatccgaca
     7261 gtggattatc cacataattg tccggatgat attttttggc aagctcccgg tatgccgctt
     7321 tcacctcgtc atcggtggcc tgcggcgaga caccaaggat cttatagggg tcattcatga
     7381 actggtcccg ctttcttttg aattcgaaaa atgtgaacta ccggataccg acaatggttt
     7441 taaaatgata atcggtcatc ggtgcgttaa cttcaaacct tggtatctca taagggtcga
     7501 tacccggctg aacggttttg ggctttgtgg taaaagccat tttgaatccg gcctgtttga
     7561 caagctgaat cgttttgtcg ctgtatttcc cgaatggata cgcaatcgaa tccgttccat
     7621 tcagtttctg cctgctctta ctaagatccg ccagaatttc agaggagctt tttgaaatga
     7681 gcatcggttt cgagcgatat tcgtcgtgca ttttctcggt gtggctgccg taatcgagcg
     7741 cgtaactgta tctggacaga tcattggtgc ttaaatactg gatggcaaaa gtcgacgtgt
     7801 tcgccggctg gtccgttgga tccaaataac cggtaatgac aaaaatcgta gcgcggaaac
     7861 catatttttt caggatcggc gcggcaaggc atccacaggt tttatagccg tcgtcaaagg
     7921 taatgagaat tttccgctcc agattcgttt tgcccttaat ataatcataa agttcatcag
     7981 gggttatcgt ttcaaaaccg gccgatttca ggattgccat ctggcggtca aattcctcgg
     8041 tggtaatcac cagaccgttt ttaaccttcc ccttctgctg agccgtcata atatcatgat
     8101 acattaaaac cttaacgccg gttccgctga ttttgggaag cgacgaggaa gaaaccggct
     8161 ccttcgaaga cgaagactca ctcactgagc tggactttac cgaggacgta ttcgccgccg
     8221 aggccaattt cgacgacgct gcggactccg ttttcttgct gccggaggaa catcccgcca
     8281 aagtgaaaac gaaaatgagc ccgaaaatca gaatccgaac caaatatgcc gcttttaaat
     8341 ttttcactct actctcctcg ttttctgtgt caattttctt ctactgtcat aggatgcgcc
     8401 gcgttttttg attttccaca ggaattcagt ttttttaaaa ccttacgctg catgccgggc
     8461 aaaccaagat agacaatatt acggagaaca gggtcgaaac ccgccgggct taaaagctca
     8521 taggcggcag taatttcggc aatagaggga ttcagcgatc tctcaatctc ctgcgtcgat
     8581 cgggccgctt tttccccttt ttgcagctgg aaagcacaga caaacggatt gtagctgtta
     8641 tctgcaagat ccttttcaag atcgtccgcc gcatccatca gataaatcca tctgcccaaa
     8701 aaatagccca atcgttccaa aatccgcttt tccgcaccgt cctggactcc gcaggaagca
     8761 atgctcgaca tcatttcccc cgtcgggcag gcggcctcat caatacttat gctctgtttt
     8821 ttctcaatct gtctctgcga ctccagatac cgactgacaa ccgactcggc ctcaggccgg
     8881 aatttcaccg cccgccggtg ggttgccgac gcaaatggca gcagtaaggc tgccgccgcc
     8941 tttcgaagcc cccgcgcgtc acgcagatta tccttcacct tatagtaata aagaatcgca
     9001 gcaacatccg ccgcaaaatt cagctcgtcc gattccacgc agcagaggca tttcaccaac
     9061 ggctggtaag ggcatcgttt tttctgaaat ccgccgcacc cggatccggc cgcaaaccgc
     9121 agcgccgcca gaaaggccgc atcatagctg agcagcatcc tgttgataaa gccatatctt
     9181 ttgccaagcg aatggcacac gccgcaataa accgcacgga aagagtcata atctttaaat
     9241 ttcatttcag gcttaaaggg cctgatataa ccaaacaaac gcttacccct cccgaacggc
     9301 tccccataca atggtcgaaa aagagtttcg acataaatct attctacatg ataggcttgg
     9361 caatttcaat gaataatatg taaaaattca ccgccgccgg tggatttccg gcggcggcgg
     9421 tgaaaagcgg ttatttcaga tgttccgcaa aaccttttcc atattcaaac gcgccattca
     9481 tttcttcctc ggaaggcaca aaacggcagg tgtagccacc ttcaaaaaca tccatattca
     9541 gcgaacgaag ccgcgagaca atgttcggaa ccgcttctcc gctccagccg aacgagccaa
     9601 aaacgccgca atgtctgcct tttgcattga tcgcatcgat gccggcgcag agtttccaaa
     9661 ccgggagaac cgcgtccttg tttatggtcg gagaaccgat cagaaacgca tcgctctgat
     9721 taattttaac ttccagatcg gcgaagtcat tttcattaat gtcataaatt ttgacctcgg
     9781 cgtcggtcat aacgctccgg acacccctca caatttcttt tgccagcgct tcggtgttgc
     9841 cgtaggcgct gcagaaaaag accggaataa acgggcgctc acgtatgaca ggccggctcc
     9901 actcgccata cctttgtttt gccgtttcca gaaacttgcc ccgcgtcagc accgggccgt
     9961 ggctcgtgca gacataatcg gcgtcaagcg tatttaactt ctcgagtccc tttagcacgt
    10021 agctcttaaa cggaccgaaa atggcgttaa aatattcatg aaacgccttt tcaaaacggt
    10081 cgaaataaac gacatggctg tcagccatac gcggctcgca gtaatgagaa ccaagaaagt
    10141 cgcaggtgaa agcaatttta tccgactcaa gccaggtgaa catcgaatcc ggccaatgga
    10201 ggaacggagc gctaaggaaa cggagaactt ttccgccgcc gatgtcaagc gtatcgccgt
    10261 ctttgacaac tttgattttc aagtcgggtt tattcgtaat atttttaaga taaatcgcgc
    10321 cggcgggcga tgtaatcacc gtgatctgcg gggccatttc aaataatctt gcaagagaac
    10381 cggaatgatc cggctcacag tgattcatga tgacataatc aaccgtcttt gggtcgatga
    10441 ccgacgcaat attttcataa tattcatcaa aataccttgg atgaaccgtc tcaatcaaca
    10501 cattcttttc tgattttatg aggtaggaat tataggaagt tccgtaatcc gtcctcataa
    10561 taacgtcaaa aatacgcata ttggggttca aaacccccac cgaataaata ccttccttga
    10621 tttttataac tcccataatt cattccgctt cctccgccgc cgcagcggat cgggtttgac
    10681 agctttccgc ggccggtctt ttgaaagctg tcaaatattg atctgacgca cctgccgtca
    10741 ggattaatat aatttgtccg tgccgttcgt tctgcgcacg cgcaaaatga cagcacggac
    10801 aaaacatctt attctcaggt aaaaattaat tactgcgctt caaacatatc cttgccgaca
    10861 ccgcacatcg ggcagacaaa atcatcgggt acatcttccc atttggttcc aggagcgatt
    10921 ccctgatcgg ggcatccttt ttcctcgtca taaacgtaac cacaaacgct gcaaacatac
    10981 tttttcataa taacactcct ttactttact tggcatcaca aattatacta tatcagtcca
    11041 catttgtaaa gcccgtttgc ctttcaatgt gccaaataat agatataatc gtattattat
    11101 tttataacgt taaaataaac atttcaatat aaattttgct ttttttgaca tttttcataa
    11161 aaataaataa aattttaaaa agcatgacgg gaaaaaggac gacgaagact tcgattctgt
    11221 gtcttcccgt ctaaaaaaaa accgccggtt tcccggcggc ggcagaaaag agattttttc
    11281 tatttgaaat ccatgaaaat catctacagg agaatgcaga tcagccctcc gcagccctcg
    11341 ttgataatcc tctccagcgt ttcctgaagt ttttcgcggg cgtccgctgg catgcggtag
    11401 agtttgttat gcaggccctc cgtgaccaga tcgtgaaggg attttccaaa aatatttgat
    11461 tcccatattt tttcaggatt ctcttcaaat tctttcagca tataggtgac aagctcttcg
    11521 gactgtttct cgtttccgac gatggggttg atctcggtta ccacccggca cttgagcata
    11581 tggatggatt ccgagcttgc cttcagcctg acgccgaacc gtccgccctg tttcacaatt
    11641 tcaggttcct cgagcttcag ctcatctacg gatggcatca cgatcccgta tccggtcgca
    11701 ttcacctcgt cgagcgcgtc tttgatcttg tcatattctt ttttcatctt tgacaaaccc
    11761 atcatggttt cgatcaggtc gccctctccg ttgatttcaa gccccgtggt ttctcccaga
    11821 accttataga acagctc
//
LOCUS       DATALY010000163         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_381074,
            whole genome shotgun sequence.
ACCESSION   DATALY010000163 DATALY010000000
VERSION     DATALY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381074"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..668)
                     /gene="cbiM"
                     /locus_tag="VHR42_04790"
     CDS             complement(54..668)
                     /gene="cbiM"
                     /locus_tag="VHR42_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HEX3026524.1"
                     /translation="MHIPDGYLSPQTTIAAFAGMLPVWAVAVKKTKKVLNEKRIPALS
                     LCAAFSFVIMMFNVPVAGGSSAHAVGAVLIAILLGPWAATISVSTALLIQALIFGDGG
                     ILAFGINCLNMGVVMPFAGYYLYKLVAGKSELGSSRNLTGAFIGGYIGINLAALCAAV
                     EFGIQPLLFKSASGVPLYCPYPLSVSIPSMNQLFPLPRFCNQFG"
     gene            909..>1763
                     /locus_tag="VHR42_04795"
     CDS             909..>1763
                     /locus_tag="VHR42_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3026525.1"
                     /translation="MKSYDAVIIGRGPAGISASLYLSRANLSTLMLGTDQSALRKASE
                     IENYFGFEGVMSGSALLDAGEKQAQRLGVEIRQEEVVALGPAETGFDIQTVSGGYAAK
                     AVLIATGQPSRKASIPGLQDFEGKGVSYCTTCDGFFYRNKSVGVLGNGNYAVQEAMEL
                     EPFTKDITIFTNGSELSVTGEFAQRLEKYDVIQKPVIRLVGEEKLGELILEDGPKKLD
                     GLFIAFGTASSVDFAVKLGVNVRDNAILVDERQMTNFDGIFAAGDCTGGFKQISTAVG
                     QGALAGRGM"
BASE COUNT          476 a          400 c          498 g          389 t
ORIGIN      
        1 atatcctgtc cgcccttgtc ggaatcgggc tgatttcggc gctgaccatc gtctcatcca
       61 aactgattgc aaaatctcgg aaggggaaat aactgattca tggaaggaat ggagaccgac
      121 agcggatagg ggcagtaaag aggaactcca cttgccgact tgaaaagcag cggctgaatg
      181 ccgaattcca cggccgcgca aagagccgcg agattgattc cgatataacc gccgataaaa
      241 gctccggtta aattgcgact gctccccagt tccgacttgc ctgctacgag cttataaagg
      301 taataacctg cgaagggcat gacaacgccc atattcaggc agttaattcc aaaagcgaga
      361 atccctccgt caccaaaaat gagagcctga atgagcaggg cggtcgagac cgatattgtg
      421 gccgcccatg gcccaagtaa aattgcaatc agcacggcgc cgaccgcgtg ggcggagctt
      481 cctcctgcga caggaacatt gaacatcatg attacaaatg aaaaggcggc gcacagggaa
      541 agagcgggga tgcgcttttc attcaaaacc ttctttgttt ttttaaccgc aacggcccag
      601 acaggcagca tgccggcaaa agcggcgatg gtggtctgag ggcttaagta accgtcggga
      661 atatgcataa acgtacctcc tgacaaaaat aaagcccatg atgggcgtca gtgcccttca
      721 tgggtcgatt tcattcaaaa tccggtttct gtggagcctt catgactccg ttaattttat
      781 tttaaccgat tttcagaaaa atttcaaggg ttgatttaat ttgaggcctt ttagatgatg
      841 aaagagtgga aaactgatat aatagattta gagaacagat taggagaatt tcgggaggtt
      901 caaaacagat gaaaagctat gatgcggtga ttatcggaag aggccccgcc ggtatttcag
      961 cttcgctcta tctgagccgg gccaatttaa gcacgctgat gttgggaacc gatcaaagcg
     1021 ccctgagaaa agcctctgag attgagaatt atttcgggtt tgaaggcgtc atgagcggat
     1081 ccgcgctgct ggatgccggg gaaaaacagg cgcagaggct gggagtggag atccgacagg
     1141 aagaggtcgt ggcgctcggc cccgccgaaa cagggtttga tatccagacg gtctcgggag
     1201 gatacgccgc gaaagcggtc ctgatcgcaa ccggccagcc gtcgcgcaaa gcctcaattc
     1261 cgggcctgca ggactttgaa ggcaaaggcg tcagctattg cacaacctgt gacggatttt
     1321 tttaccgtaa taaatccgtc ggtgttctgg gcaatgggaa ttatgcggtt caggaagcca
     1381 tggaactgga gcctttcacc aaagatatca cgatttttac caatggaagc gagctttcgg
     1441 taaccggtga atttgcacaa aggttagaaa aatatgatgt gattcaaaaa ccggtcatcc
     1501 ggcttgtggg ggaagaaaag cttggcgagc tcattctgga agacggcccg aagaaactcg
     1561 acggactgtt tatcgcgttt ggaacagcat ccagcgttga ttttgcggtc aagcttgggg
     1621 tcaacgtcag agacaacgcc attctggtcg acgaacggca gatgaccaat tttgacggga
     1681 tttttgcggc gggagactgc accggaggtt ttaagcagat ttcaaccgcg gtcgggcagg
     1741 gcgcgttggc cggcagggga atg
//
LOCUS       DATALY010000164         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_641036,
            whole genome shotgun sequence.
ACCESSION   DATALY010000164 DATALY010000000
VERSION     DATALY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641036"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VHR42_04800"
     CDS             <1..877
                     /locus_tag="VHR42_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3026526.1"
                     /translation="VDVGLEGLLRRLGVAKPLKLILKPFKVGLPSKLILWDEVETIDF
                     GHAGIKLSTVYSKLETLHASDLADIIEDMDSKRQAEIFASLNEERAADVLEELETDAQ
                     INVLEQLSIERAADVLEKMPADEVADILEEISDERAERLLSEMESVSSEEVRELMEYD
                     EDEVGSLMSTDFLSFGKNMTVSETFQELRRLKPESDEIYYLYLVDHFGKLIANVSLRD
                     LVISQPETRLQDIMNEKVVFVHDTDELDSVAEIISKYSLLAVPVVNKNMVMIGMVIID
                     DVVYTLLKSHRKRGL"
     gene            906..>1885
                     /locus_tag="VHR42_04805"
     CDS             906..>1885
                     /locus_tag="VHR42_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3026527.1"
                     /translation="MAAIKPANKTSKKKVFTNILFFLSILGPGIITGSVDNDVGGITT
                     YSVAGATFGNKLIWTLIPAFVVLFIVQEMNARMGIVTGKGLADLIRENAGVKITFFIF
                     IFLLGADIMNTTTEFAGVAGSMQIFGVSKYIAVPLVAVIIWFLVVRGTYRSAEKIFLA
                     FSACLLIYVVAALLGHPNWSEIGGSIIHPEFKADFGYITMVISLIGTTIAPWMQFYMQ
                     SSVIEKGLKIKDYKFTILDIFVGCVATIAVAFFIVVACSSTLYVNGVGTKINDAGDAA
                     LALRPLAGQLASQVFAFGLFIAAIFSATILPVATAFYVCEAFGFEAGIDK"
BASE COUNT          453 a          392 c          512 g          528 t
ORIGIN      
        1 ggtcgatgtc ggtcttgaag gtcttttgcg gcgcctcggc gtggccaaac cgcttaaatt
       61 gatactcaag ccttttaaag ttggtcttcc gagcaaactg attttatggg acgaggtgga
      121 aacgatcgat tttggccatg ccggaatcaa actgtccacc gtttattcca agcttgaaac
      181 ccttcacgca tcggatcttg ccgatattat tgaggatatg gattccaagc ggcaggccga
      241 aatattcgcg tccctgaacg aagaaagggc cgcggatgtt ctggaggagc tcgaaaccga
      301 cgcgcagatt aatgttttgg agcagctgtc aattgaaaga gcggcggatg ttctggaaaa
      361 aatgcctgcg gacgaagtgg cggatattct tgaggaaatt tccgacgagc gcgccgagcg
      421 tctgctgtcg gaaatggaaa gcgtttcatc cgaggaagtt cgcgagctca tggagtatga
      481 tgaggatgag gtaggcagtc tgatgtccac tgatttcctg tcgtttggca aaaacatgac
      541 ggtcagcgag acgtttcaag agctgagaag gttgaagccg gaatcggatg agatttatta
      601 tctgtatctt gtcgatcatt ttggtaagct gattgcgaat gtgtcgctga gggatcttgt
      661 aatttcccag ccggaaacaa gacttcagga catcatgaat gaaaaagttg ttttcgtgca
      721 tgatacagat gagttggatt cggtggcgga aattatttcg aaatacagcc tgctggcggt
      781 gccggtcgtt aataaaaata tggtgatgat cggaatggtc atcattgatg acgtggttta
      841 cacattgctg aagtcgcacc ggaaacgcgg cttatagatc attgataaaa aggagggtga
      901 tttcaatggc agcgatcaaa ccagccaaca aaacctccaa gaaaaaagtt tttaccaata
      961 tattgttctt tttgtccatc ctcgggcctg ggattataac gggcagcgtc gacaatgatg
     1021 tcggcggaat caccacctat tcggtcgcgg gtgcgacttt tggaaacaag ctgatctgga
     1081 cgctgatccc cgcttttgtg gttctgttta tcgttcagga aatgaacgcg agaatgggta
     1141 ttgtcacagg caaaggtctg gccgatctga tccgtgaaaa cgccggggta aagattactt
     1201 tcttcatatt tatttttctg ctcggcgcgg atattatgaa tacaacgacc gaattcgccg
     1261 gcgttgccgg aagcatgcag atatttggtg tcagcaaata tattgcggtt ccgcttgtgg
     1321 cggtgattat ctggtttctt gtggtgaggg gtacttatcg ctcggccgaa aagatttttc
     1381 tggcgttcag cgcttgtctt cttatttatg tcgtcgcggc gcttttgggg catcccaatt
     1441 ggtcggaaat cggcggcagc attatccatc cggaattcaa agctgatttc ggctatatca
     1501 cgatggtgat ttcattgatt ggtacgacga ttgctccgtg gatgcaattt tatatgcagt
     1561 catcggtcat cgaaaaggga ttgaaaatca aagactacaa atttacgatt ctggatatct
     1621 ttgtaggctg cgtagctacc attgccgtgg cgtttttcat tgtggtcgcc tgctcttcga
     1681 ccctttatgt caacggggtt ggcacgaaaa tcaacgatgc gggtgacgcc gcacttgcgc
     1741 tgagaccact ggccgggcag ctggcatcac aggttttcgc atttggcctt tttatcgccg
     1801 ctattttttc ggcaaccatt ctccccgtgg ctactgcgtt ctatgtctgc gaagcatttg
     1861 gatttgaagc cgggatcgac aagaa
//
LOCUS       DATALY010000165         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_468654,
            whole genome shotgun sequence.
ACCESSION   DATALY010000165 DATALY010000000
VERSION     DATALY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468654"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..>1859
                     /locus_tag="VHR42_04810"
     CDS             214..>1859
                     /locus_tag="VHR42_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3026528.1"
                     /translation="MKRRIVFILTLIFSLAINLVSMHYGIFNIAQHLFYITIILGVFF
                     FEKKGVFLSLFVSALYVGTVLIYDSDYSDLPQTVVRAFILIFIAVLIDWMIARYKNKE
                     SEIEAFFNINAEMMCVSGHDGNFLRVNRAFEDVLGYGKEEFEHYSFTELIHPDDLVTT
                     SKAMARLKDSGQVTEYVNRCRCRDGSYKYIEWRSFPFRDVVYSTARDITERTRLIHQL
                     KSNENRMKFAEDLANIGSWEMNPETEEFRWSQGMYRILGLNPGLSMPGIGLMKELIDP
                     DDWENVEKIFKRNSGNPNDFDFEARFRRPDGNLRWIHSKGMLFLDGAGEKQKYFGFFM
                     DITDRKAAEKIQKQNLAGYKSLMEIYQHKADSEKELLTYIVSQAVGLTGSRGGAIYFC
                     DEKAGRMFMAAGAGEDQIRFGKSVAFDASCAAARAYRERRRIMLNRASGDVGGADKSP
                     PMLAVPVFDGESVVAVAAVADKTLAYDESDGWLLLMMMRNVWELVLRRRTELRFFRER
                     QWFETTLLSIGDGVIATDADGIIRLVNKKAETLTGFPKED"
BASE COUNT          497 a          351 c          508 g          503 t
ORIGIN      
        1 attgttccaa tatcatacat atgcaggtag cggcgcggtt catcacttcg gataaattat
       61 gaatttttgg tttgaaactc aggattatat gttaaaatag aagacacaac agataagccg
      121 aaatagggcg gaatagattg cttttttaaa atcgcgcttt catgttagac aagttttatt
      181 ctcagcgaaa tacatagaat gcgaggatca actatgaaaa gacggatcgt ttttattttg
      241 acgctgattt tttcgctggc aatcaatctt gtaagtatgc attatgggat tttcaacatt
      301 gctcagcacc tgttttatat cacgattatt ctgggcgtct ttttctttga gaaaaaaggc
      361 gtatttctgt cgttgtttgt ctccgctctc tatgtcggaa cagttttgat ttatgacagc
      421 gattattccg atttgccgca gactgtggtc cgggcattta ttttgatatt tattgcggta
      481 ttgattgatt ggatgatagc gagatataaa aacaaggagt cggagattga agcgtttttc
      541 aatattaacg ccgaaatgat gtgcgtttcc ggccatgacg gaaattttct gcgggtcaac
      601 cgggcttttg aggatgttct gggttacggc aaagaagaat ttgaacatta cagctttacc
      661 gaactgattc atcccgatga tcttgtgact acttcaaagg caatggcaag acttaaagac
      721 agcgggcagg tcaccgagta tgtaaatcgg tgcaggtgca gggatggaag ctataaatat
      781 atcgaatggc gttctttccc tttcagggat gtggtttatt ccaccgcccg ggacatcacc
      841 gagcgtacca gactgatcca tcagctgaaa agtaacgaaa acaggatgaa gtttgcggag
      901 gatctggcta atatcggcag ttgggaaatg aacccggaaa ccgaagaatt ccggtggtcg
      961 caggggatgt atcggatttt gggactgaac cccggattat ccatgccggg aatcggtctg
     1021 atgaaagagc taattgatcc ggacgactgg gaaaacgtcg aaaaaatatt caaaagaaat
     1081 tccggaaatc caaacgactt tgattttgaa gcccggtttc ggcgcccgga cgggaatctc
     1141 cgctggattc attccaaagg tatgcttttc ctggatggtg ccggtgaaaa gcagaaatac
     1201 tttggatttt tcatggatat taccgaccgg aaggctgcgg agaagattca aaagcaaaat
     1261 ctggccggtt ataaaagcct gatggagatt tatcagcata aggcggattc cgagaaagag
     1321 ctgctgacct atattgtttc ccaggcagtc ggccttaccg gcagccgggg gggcgccatt
     1381 tatttctgtg atgaaaaagc cggccgcatg tttatggcag ccggagcggg agaggatcag
     1441 attcgtttcg ggaaatccgt tgcgtttgac gcatcgtgtg cggctgcccg ggcctatcgg
     1501 gagagacggc ggatcatgct caaccgggcg tccggtgacg tcggaggtgc ggataagagc
     1561 cctccgatgc tggctgttcc tgtatttgac ggggaatcag ttgttgcggt ggctgcggtg
     1621 gcagataaga cgcttgcata tgatgagtca gatggctggc tcctgttgat gatgatgcgc
     1681 aatgtctggg aactggtact tagaagaagg acggagctga gattttttcg ggagagacaa
     1741 tggtttgaaa cgactttgct gtcaatcgga gacggtgtga ttgcaacaga tgcagacgga
     1801 atcatacggc ttgtgaataa aaaagccgag acgctgacgg gttttccaaa agaagacaa
//
LOCUS       DATALY010000166         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_468933,
            whole genome shotgun sequence.
ACCESSION   DATALY010000166 DATALY010000000
VERSION     DATALY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468933"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..648
                     /locus_tag="VHR42_04815"
     CDS             166..648
                     /locus_tag="VHR42_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3026529.1"
                     /translation="MDKILTILEENAKLTDEQVAAMLGTTASEVRDAVHRYEQEGVIK
                     GYKALIDWDKTERDLITAFIEVKVSPQRDSGFEQIAERIMQFDEVSSVNLMSGGFDLA
                     VTVEGASFKEVAMFVSQRLSPIDNVLSTATHFVLRKYKDKGVMFGAEKPDERGKISLC
                     "
     gene            642..1808
                     /locus_tag="VHR42_04820"
     CDS             642..1808
                     /locus_tag="VHR42_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3026530.1"
                     /translation="MLEYQRIVSDRVREIKPSGIRKFFDIVNEMDDVISLGVGEPDFA
                     TPWHVRSAGIYSLEKGKTWYTSNAGLPQLREEICTYLERRFGVSYHPEKEVVVTVGGS
                     EAIDMAIRTVVSPGDEVLIPEPCFVCYYPLTQLAGGVPVPLKTKAEDQFRVTARAVKE
                     AITPKTKMLILPYPNNPTGAVMTRQDLADIADVLKGTDIIVLSDEIYGELTYDGKHVS
                     VPSVPGMKERTLLVNGFSKAYAMTGWRLGYVCGPSELIGYMNKVHQFAIMCAPTTSQY
                     AAVEALKNGDEDIERMATEYDMRRRFVLNGLKKAGLECFEPKGAFYVFPSIRVSGLSS
                     EQFCERLLYEKKVALVPGNAFGDNGEGYVRISYSYSVRHLTEALKRIDQFVKSL"
     gene            1851..>2909
                     /locus_tag="VHR42_04825"
     CDS             1851..>2909
                     /locus_tag="VHR42_04825"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX3026531.1"
                     /translation="MEKVSVIGCREYDPDRLVRIIEEHFCALDPENKLIRKGSRVVVK
                     PNLIMRRKPEEATTTHPDFVAAVIRAVKKRGGEAVIADSPGGPYTKSQLRSIYAGTGM
                     TQAAEREGAELNDDVGFTAMMSKDGKVCRAFNIINPLAQADVIISVAKLKTHGQMNFS
                     GAVKNMFGAIPGLTKPEFHYRYPNKNTFGEMLVDLCETVRPAISLIDGVIGMEGNGPT
                     GGSPKFMGVTMASLNPYAMDLIACSVIGMPASMVPTVAASQKRGLCPDHADEIEILGE
                     DFNHFASDFQKPESMSLDFIHKMPGFIRKPLTRVFTPKPVIKKATCIGCGKCAESCPQ
                     KTISIIERKAVIDYTECIK"
BASE COUNT          731 a          674 c          818 g          686 t
ORIGIN      
        1 gttgttcatg aaaggagcgc aaattgatga gaaaatgtca ggcagcctgc ctgcggccct
       61 taacaattga atccggcaga ccgacggtgt ataattttct tataatttat gtccggttat
      121 gaattttgcc tgtaaaaatg attcgaacgt caggagagtg ttggaatgga taagatatta
      181 acgattttgg aagaaaacgc aaaattgacc gacgaacagg tcgcggcgat gctgggtaca
      241 accgcctctg aagtcaggga tgcggttcac agatatgagc aggaaggcgt catcaaaggt
      301 tataaggcgc tgattgactg ggataagacc gaaagagacc tgattaccgc ttttattgaa
      361 gtcaaggtct ctccccagag ggattccggt tttgaacaga tcgccgagag aatcatgcag
      421 tttgacgagg tgagcagtgt taatctgatg tcgggcggct ttgatcttgc ggtaacggtg
      481 gaaggcgctt cgttcaaaga agtcgccatg tttgtttctc agaggctttc ccccatcgac
      541 aacgttttgt caaccgcgac gcattttgtc ttacggaaat acaaggacaa gggcgtaatg
      601 ttcggagcgg agaagccgga cgagaggggg aaaatttcgc tgtgctagag tatcagcgga
      661 ttgtttccga cagggtcaga gaaatcaagc cgtcgggtat ccgaaaattt tttgatattg
      721 taaatgaaat ggatgatgta atttcacttg gcgtgggaga acctgatttt gccacgccgt
      781 ggcatgtgcg cagcgccggg atttattcgc ttgaaaaagg aaagacctgg tatacctcca
      841 acgcgggcct accgcagctc agggaagaaa tctgcaccta tctcgaacgc agattcgggg
      901 taagttatca cccggaaaaa gaggttgtcg taacggtcgg cggaagcgag gccatcgaca
      961 tggcgatccg gacggtcgta tctccgggcg atgaagtgct gatccccgag ccgtgttttg
     1021 tatgttatta tcctctcact cagcttgcgg ggggagttcc ggtcccgctg aaaacgaaag
     1081 cggaagatca gttccgggtt accgcccggg cggtgaagga agccatcact ccaaaaacaa
     1141 aaatgctcat tctgccttat cccaacaatc ccaccggggc ggtcatgacc cggcaggatt
     1201 tggctgacat tgccgatgtg ctcaaaggaa ccgacattat cgtgctttcc gatgaaatct
     1261 acggtgagct tacttacgac ggaaaacatg tttccgttcc gtctgttcct gggatgaaag
     1321 aaagaacgct gctggtcaac ggtttttcaa aggcttatgc catgaccggc tggaggcttg
     1381 gttatgtctg cgggccgagt gagctgatcg gctatatgaa taaagtccac cagttcgcca
     1441 ttatgtgcgc gcctaccacc agccagtatg cggcggtgga ggcgctgaaa aacggcgatg
     1501 aagatattga acggatggcc accgaatacg atatgcgcag aaggttcgtt ttaaacggcc
     1561 tgaaaaaggc ggggctggaa tgttttgagc caaaaggcgc tttttatgtt ttcccgtcca
     1621 ttcgggtgtc ggggctgagc tccgagcagt tctgcgagcg gcttttgtat gagaaaaagg
     1681 tggcgctggt tcccggcaat gcgttcggcg acaacggaga gggctatgtg cggatttcct
     1741 acagctattc cgtccgtcat ttaacagagg ccctgaaacg tattgatcaa tttgtgaaaa
     1801 gcctgtaacg aaaacgccgg agcggccttt tcggatgaaa ggaagacgtt atggagaagg
     1861 tatctgtcat tggctgccgg gaatatgatc ccgaccggct tgtcaggatc atcgaagagc
     1921 atttctgcgc tcttgacccg gaaaataagc tgatccggaa aggaagccgt gttgtcgtca
     1981 agcccaacct gattatgcgc cgaaagccgg aggaggctac gacgacccat ccggattttg
     2041 tcgccgctgt gatccgcgcg gtcaaaaaaa gaggcggcga agcggtgatt gccgacagcc
     2101 ccggcgggcc ttatacaaaa tcccagctcc gttcgattta tgccggcact ggcatgactc
     2161 aggcggcgga aagggaaggc gccgaattga acgacgatgt cggatttacc gcgatgatgt
     2221 ccaaagacgg taaggtttgc agagccttta acattattaa cccgcttgcg caggctgatg
     2281 ttattatttc ggtggccaaa ctcaaaacgc atggtcagat gaatttttcg ggcgccgtca
     2341 aaaatatgtt cggcgcgatt ccggggctaa ccaaacccga atttcattac cgttatccga
     2401 ataagaacac ctttggcgaa atgctggttg atctttgtga aaccgtccgg cccgctattt
     2461 ctctgattga cggcgttatt ggcatggagg gcaacggccc caccgggggg agcccgaaat
     2521 ttatgggcgt gaccatggcg tcgctgaacc cctacgcgat ggatttgatc gcctgctccg
     2581 tgatcggcat gccggcgtcg atggttccga ccgtggcggc ttcccagaag cgcgggcttt
     2641 gcccggacca tgccgacgag attgaaatcc ttggggaaga ttttaatcat ttcgcgtccg
     2701 attttcagaa accggaatcc atgtcgctgg atttcattca taagatgccc ggtttcatcc
     2761 gaaaaccgct taccagagtt tttactccca aacccgttat taaaaaagca acctgcatcg
     2821 gctgcggcaa atgtgccgag agctgtccgc aaaaaacaat cagcattatc gaacgcaagg
     2881 cggttattga ctacacagaa tgcatcaaa
//
LOCUS       DATALY010000167        14373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_211253,
            whole genome shotgun sequence.
ACCESSION   DATALY010000167 DATALY010000000
VERSION     DATALY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211253"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VHR42_04830"
     CDS             <1..755
                     /locus_tag="VHR42_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026532.1"
                     /translation="FPDQTGSAEHDCEQNEKRGFEYLNAIFFRRHRRLMIRPVMIRLG
                     ITGALLIAAIAASLLLPGFEKQMRGLQNNLLPVFVFVMYFASIGEKICKAMFYNCDVS
                     LLRYPFYRNKNAVLHNFKIRLFWVAGLNFITACAVSAAVVCFFVITKIGLPVEGMAWF
                     VLSILTLSLFFSVHHLFLYYVFQPFTSELGMKNPFYGIINYIVYMLCFFCLKIKSAPS
                     YFTMIVLVSTVLYIIVALIIVYKFAPRTFRVK"
     gene            complement(763..1620)
                     /locus_tag="VHR42_04835"
     CDS             complement(763..1620)
                     /locus_tag="VHR42_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX3026533.1"
                     /translation="MTHKALKARTITVRILLILIGATLSSIGLEIFLVPNNIIDGGVI
                     GISIISSYLTHLPLGLFTFALNIPFFMIGYKQIGKSFVAATLFAVICLSVGVSFLHPI
                     PGITHDVFLAAVFGGIINGAGVGLIIRAGGSLDGAEIVAIILDKKISFSVGEIVMFFN
                     IFILVGAGFLYGWDRTMYSLMTYFITFKVIDIVIEGIDESKAVMIVSDKDEDITEAVI
                     ARLGRGVTLLNGKGGFSKTPVNVIYLVVSRLELTKLKSIVLDFDENALITVGNVEVAG
                     KKYKKNAIH"
     gene            1904..2065
                     /locus_tag="VHR42_04840"
     CDS             1904..2065
                     /locus_tag="VHR42_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003658257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX3026534.1"
                     /translation="MKTYQCSVCGYLYNEELGCPDQGIVPGTKWEDIPDDFICPECGV
                     LKGMFDPVQ"
     gene            2104..3390
                     /gene="eno"
                     /locus_tag="VHR42_04845"
     CDS             2104..3390
                     /gene="eno"
                     /locus_tag="VHR42_04845"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX3026535.1"
                     /translation="MRKSTSIELVSAREILDSRANPTIETEVVLADGSKGVAAVPSGA
                     STGMFEAVELRDSDPARYGGNGVLKAVENVNTDIFNALKGMDASAQREIDSRMIELDA
                     TENKSRLGANAILSVSLAAAKAAAESAQMSLYRYIGGCNASVLPVPMMNIINGGAHAS
                     NNVDIQEFLIMPVGAPDFAEAIRWCSEVFHSLKKVLSGRGLSTAVGDEGGFAPDLKDD
                     EDALRVILEAVEKAGYQPGSQFRIAMDPASSEWYQENGIYLLPKKQKKMTSGELVDFW
                     DRLVKDYPIVSLEDGLAEEDWQGWKLLTQRLGDQVQLVGDDLFVTNTGRLEKGISLGV
                     ANSILIKLNQIGTLSETLEAIRKAHRAGYTAVISHRSGETEDTTIADLAVAVNAGQIK
                     TGAPSRSERVAKYNRLLQIGRELGSAAQYPGMSVFS"
     gene            3499..3888
                     /locus_tag="VHR42_04850"
     CDS             3499..3888
                     /locus_tag="VHR42_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HEX3026536.1"
                     /translation="MKEILFYRCEKCGNIVALLKKGGGTLSCCGQDMTRLKENSTDAA
                     TEKHVPIVAKDGGKVKVTVGSVAHPMAPEHFIEWIALSCGDRLEIVFLKPGDEPKAEF
                     TCPDGSSCGTAYAYCNLHGLWKADCNA"
     gene            4126..5085
                     /locus_tag="VHR42_04855"
     CDS             4126..5085
                     /locus_tag="VHR42_04855"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX3026537.1"
                     /translation="MRTDRRKVVLVGTGFVGMSYAYAMLNQNACDELVLVDIDRKRAE
                     GEAMDLNHGLAFSGSHMKIYAGGYEDCRDADIVAIAAGVAQRPGESRLDLLQRNTKVF
                     GSIINPVVESGFKGIFLIATNPVDIMAHVTKTISGFDRKRVIGTGTTLDTARLRYMLG
                     EYFQVDPRNVHAYVMGEHGDSEFVPWSQALIATKPALELCGQSNGQYSCDCMSRISAE
                     VKNAAQKIIEAKQSTYYGIGMSMVRISKAILGDENSVLTVSTLLDGEYGRSDVYVGVP
                     AIVSRNGVEKIITLSLTDGELKQFHDSCDLLIQTYHGINFELL"
     gene            5129..5641
                     /locus_tag="VHR42_04860"
     CDS             5129..5641
                     /locus_tag="VHR42_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX3026538.1"
                     /translation="MLNNEIVTKLNEQLNKELYSTYFYLDMSNYYFHQNLDGFGNWFY
                     VQAQEERDHAMLFNKYLLNNDQEVVLTDVKASGVSFSDFREPLSKTLEHERFITASIN
                     DIYAVAYTQKDFRTMQFLDWFVKEQGEEEKNADDLVKKFDLFGKDSRGLYLLDTELKA
                     RVYTPATLVL"
     gene            complement(5706..6956)
                     /locus_tag="VHR42_04865"
     CDS             complement(5706..6956)
                     /locus_tag="VHR42_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018962531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3026539.1"
                     /translation="MKTQKKSHKPRLAALDFFKYIGPGLIVTVGFIDPGNWASNISAG
                     AQYGYVLLWMVTLSTIMLIVLQHNSAHLGIVTGKCLSEAATEHLRPWLKNFILATAML
                     AAVSTAMAELLGGAIALNMLFHLPIKLGTVLILAVVLWMLFTNSYRKIEKWIIGFVSI
                     IGISFIFELTLVPVDWGKAASGWITPAIPGGSLPIIMSVLGAVVMPHNLFLHSEIIQS
                     RQWNLQDESVIRKQLAFEFGDTLFSMMIGWAINSAMILIAATVFFTQKAQVSELSQAQ
                     LLLKPMLGNAAAAIFAVALLFSGISSCVTAGMAGGSIYAGVFSEPYDISDSHTKTGVL
                     MTMILAAAVIFFITSPFDGLVYSQMLLSVQLPFTIFTLIYLTSSKKVMGKYANSGFTR
                     VLLWGIGLTVTVLNIALFLSYARR"
     gene            7376..8317
                     /gene="mmuM"
                     /locus_tag="VHR42_04870"
     CDS             7376..8317
                     /gene="mmuM"
                     /locus_tag="VHR42_04870"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEX3026540.1"
                     /translation="MDPVSKILKSFPCIVLDGALATELERRGCEINDALWSAKILAED
                     PELIQKVHYDYFVSGADCAITASYQATIQGFIRKGFSVEESEALIRRSVIVAKKARDD
                     FWKDESNRMNRTKPFVAGSIGPYGAYLADGSEYRGDYRISEDELIEFHRPRIRLLLDE
                     GVDLLACETIPSLIEAQAIVKLLEEFSEVSCWVSFSAKNGYEISDGTPILECAGYLET
                     CGQVAAIGVNCTPPGYMKSLIGEIRKNSKKPVVVYPNSGEEYDARTKTWHGSSGGGSF
                     GCCAEDWFKAGARLIGGCCRTRPEDIRAIASWARNKP"
     gene            complement(8403..9575)
                     /locus_tag="VHR42_04875"
     CDS             complement(8403..9575)
                     /locus_tag="VHR42_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026541.1"
                     /translation="MATSEELHTAGQDTEHAKCPSCGGEMAFDPDSGALNCPYCGKNQ
                     EIISSQDINCHDFAYTGEELEQKEDPGVLLHCNNCGANIVVGPNTSADFCTFCGSPMM
                     AKAENSKAMVPDGIVPFRISQKAASSLFKDWIRGRFFSPGKLKRQNQPGRLQGAYIPH
                     FAYDCTTSSDYTAEAGEHYYVTESVTVERDGKQVQEMQEVQKTRWYSVSGTHDDAFDN
                     VLVNASKHVNQKLIKMQFDLRDLRPFQKDYIYGFVAESNSISKDECWTTARGIIEDEI
                     RNNISDSIEADEVRNVNFRTDYQDVRFKQVLLPLWISAYTFKGKTYHFMINGQTGDVK
                     GQAPLSFLRILSSILLTLGVSALFYQWKPTAGVIAFIVSIITLMILANRKPKKSST"
     gene            complement(9586..10725)
                     /locus_tag="VHR42_04880"
     CDS             complement(9586..10725)
                     /locus_tag="VHR42_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3026542.1"
                     /translation="MGILNFVKSQFIEVIEWVDDSQDTMVYRFPVENKEIKMGAKLTV
                     RESQLAIFVNEGKAADVFSPGLYTLETNNLPILTKLRSWPYGFNSPFKAEVYFFNTRQ
                     YTDQKWGTENPIMLRDAEFGMLRLRGFGIYSFRISNPLVFLQQISGTNGLFDTQSITG
                     QLKRVIVSGLTDLLGELKIPALDLASNYDEISEQVQAKLSPKFEAMGLTLVSFYVENI
                     SLPEEVEKTLDTRTQMGMLGNLQQYAQFQAANSIPDAAKNPGGLAGAGIGLGAGAVMG
                     SAFMQAFNQPAQTVVSQKSVETFVKCPSCGAQVTAGTKFCPECGKPIVPAGVKCVKCG
                     APLKDGSKFCSECGAAQNVNPVCKNCSKQLEPGAKFCPDCGTKVE"
     gene            complement(10965..11582)
                     /locus_tag="VHR42_04885"
     CDS             complement(10965..11582)
                     /locus_tag="VHR42_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3026543.1"
                     /translation="MQYLKYDVKNRIITAARDEFKEKGYSSASMRRISQNAGAALGNL
                     YRYFKNKEDLFDSLVGPVCDNIYEFIAKIRNVPTAPSDPNAPAGPVQLREINAFKEDL
                     LEICKDHNTELFILMNKSRGSKFENVKSDMISIISDILTHQLLIPAEKKGIVIRDGYL
                     ATVLSTSLVEGFSLIIRKYENGEDAKMLTDQLINIIFTDIENRIG"
     gene            complement(11650..13302)
                     /locus_tag="VHR42_04890"
     CDS             complement(11650..13302)
                     /locus_tag="VHR42_04890"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HEX3026544.1"
                     /translation="MNTDNSEKSHAVYITGHKNPDTDSICSAIAYADFKRMLHIDAIP
                     VRTGKINRETKFVLNYFGLDAPEYLATVKTQVSDLNMDRIEPVLPDTSIQQAWKKLKA
                     SNLEVLPIEDDRQILQGIVSVSDIANEYMNMPDNNALSDSSTPIGNLIRTLDAEPICV
                     FGKSLDDSGKVIIAAMTPDGMDEFIEKGDIVFTGNIKVNQIKAIEAGASCVIVTCGSR
                     VSEDVVRLAEAHQCTLLITKHDTFTGARLIYQSIPVGFTMTSENLVLFHIDDFIDDVR
                     EKLLQTRFRSYPVVDENRHFKGFISRYHLLNPRKKKLILVDHSEKAQSVNGIEQAEIL
                     EIIDHHRIGDIQTGSPIFYRNEPTGSTATIIANLYSEQGVKPSPQIAGILCAAILSDT
                     IHFKSPTSTHADLITAERLEKLAGIDMEDFSAKMFEAGSSLKNLTTEEMIQNDFKEYQ
                     IGKHKIGIGQVNTTDLSSLKKLRQLILTCLNDLAAREHFGILLILFTDILEEETEVLF
                     VESQKGLVARAFSPLQDENSFLMSGVVSRKKQIVPRLVLALG"
     gene            13633..14064
                     /locus_tag="VHR42_04895"
     CDS             13633..14064
                     /locus_tag="VHR42_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026545.1"
                     /translation="MSVNPILSTGSLTSLITMQSSLKTASAVKGAQNELQTEALIYKP
                     SFGFQGDPKKAAELSSKADSLNGKIGEICSDVQKEIQKTNDQNADSSTVQTQTDSQST
                     ADKTDPAETVASAGGQLSVSPSEQNTGDKLLSTGKNVDVNA"
     gene            complement(14149..>14373)
                     /locus_tag="VHR42_04900"
     CDS             complement(14149..>14373)
                     /locus_tag="VHR42_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026546.1"
                     /translation="PGGIMVLATPIKDSVFNRQYTSWWYEPAHLTFFEKDVMIKCAED
                     AHFDVSEIAYSEQGIGRLEFYCKKLSEPVQ"
BASE COUNT         3742 a         3244 c         3597 g         3790 t
ORIGIN      
        1 gcttccccga ccagacggga agcgccgagc atgactgtga gcagaatgag aaacgggggt
       61 tcgaatatct taacgccatc ttttttcgcc ggcaccgccg tttaatgatc aggcctgtga
      121 tgattcgtct gggcattacc ggagcgcttt taatcgccgc gattgcggct tccctacttc
      181 tgcccggatt tgaaaagcag atgcgcggtt tacaaaacaa ccttttgccc gtttttgtct
      241 ttgtcatgta ttttgcgtcg attggggaaa agatttgcaa agctatgttt tacaactgcg
      301 acgtcagtct gctgcgctat cctttttacc ggaataaaaa tgcggttctc cacaatttca
      361 aaatcaggct tttttgggta gcgggtctga actttatcac ggcgtgcgct gtttccgctg
      421 cagtcgtgtg ttttttcgtc attacaaaga ttggtcttcc tgtggaggga atggcctggt
      481 tcgtgctgtc cattctgacc ctttccctgt ttttctcggt tcaccatctt tttttatatt
      541 atgtgttcca gccctttact tcggaacttg gaatgaagaa ccctttttac gggattatta
      601 actatattgt ttatatgctc tgctttttct gcctaaaaat caaaagcgcg ccttcctatt
      661 ttaccatgat cgttttggtc tcgacggttc tgtatattat cgtggcgctg attattgttt
      721 ataaattcgc gcccagaact tttcgggtaa aataatgtgc atttaatgga tggcattttt
      781 cttgtatttt ttcccggcaa cttccacgtt gccgacggtt atcagggcat tttcgtcaaa
      841 atcaagcaca atcgacttga gcttagtcag ctcaagcctt gacacgacaa gataaatcac
      901 gttgaccggc gtctttgaga aaccgccttt gccgttcagc agggtaacgc ctcggccgag
      961 acgggcaatt acggcctcgg ttatgtcttc atctttgtcg gatacaatca taaccgcttt
     1021 ggattcgtcg ataccttcga tgacaatgtc gataacctta aaggtgataa aataggtcat
     1081 cagcgaatac atggtacggt cccatccgta taggaatccg gctccgacca gaataaagat
     1141 gttaaagaac atgacaattt cgcccaccga aaaactgatc ttcttgtcga ggatgatggc
     1201 gacaatctct gctccgtcga gcgatccgcc cgccctgatg atcaggccga caccggcgcc
     1261 gttgatgata ccgccgaata cggccgccag aaatacatca tgggtaatac ctggaatggg
     1321 gtgcagaaat gagacgccga ccgacaggca gatgactgca aataatgtgg cggcgacaaa
     1381 agatttcccg atttgcttat agccgatcat aaaaaatgga atgttgagcg cgaaggtgaa
     1441 aagaccgaga ggcagatggg tcagatagct tgaaatgatc gagataccaa tgacgcctcc
     1501 gtcaatgatg ttgtttggaa cgagaaaaat ttccagaccg attgatgata gggtggctcc
     1561 gatcagaatc agcagaattc ttacggtgat tgtccgtgct ttaagggctt tgtgagtcat
     1621 taacattacc tccgttcgca aattccggta agtttccaca acttgtttta ttatagcatt
     1681 accgtcctgt ttgaatcaaa tttacgctaa gagttggaat ttttaataaa aattggccgg
     1741 atagttgaca aacgggatgt tctctgctat ccttaaatta tacaaaaaag gtatattatt
     1801 atgattgagt aatatctgtc aaatacccac aaaataaaag gtaataattc tttacaaact
     1861 gaatattatg tatgataata atggtaaaaa ggactgatga tttatgaaaa cataccagtg
     1921 cagcgtttgc ggttatcttt acaatgaaga attgggctgc ccggatcagg ggattgttcc
     1981 cggaaccaaa tgggaagata ttcccgatga ttttatctgt cctgaatgcg gcgttttaaa
     2041 agggatgttt gaccccgtac agtaatgatg aaatattaaa aaagacaagg agagtgatac
     2101 caaatgagga aatcgacatc gattgaattg gtcagtgcgc gtgagattct tgattcacgt
     2161 gcaaatccca ccattgaaac tgaagtcgtt ttggcggacg gctcgaaggg ggtagccgcg
     2221 gttccctcgg gtgcgtcgac cggcatgttt gaagcggttg aactgcggga ctcggatccc
     2281 gccagatatg ggggaaacgg tgtcttaaag gcggttgaga acgtcaacac agatattttt
     2341 aacgcgctaa aaggtatgga cgcctcggct cagcgggaaa ttgacagcag gatgatcgag
     2401 cttgacgcga ccgaaaataa atcccggttg ggagcgaacg caattttgtc tgtgtctctt
     2461 gccgcagcga aagcggcagc ggaatccgct caaatgtctc tctatcgtta tatcggagga
     2521 tgcaacgcct ccgttttgcc cgtgccaatg atgaatatca taaacggcgg ggcgcatgca
     2581 tccaataatg ttgacatcca ggaattcctc atcatgccgg tcggcgctcc ggatttcgcc
     2641 gaggccattc gctggtgctc cgaagttttc cacagcctga agaaagttct ttccggcaga
     2701 ggtctgtcaa ccgccgtggg ggacgagggc ggttttgcgc ctgatctgaa agatgatgag
     2761 gatgcgcttc gcgtgattct ggaagcagta gaaaaggcgg gttatcagcc cggcagccag
     2821 ttccgtatcg ccatggaccc ggcgtcaagc gaatggtatc aggagaatgg aatctatctg
     2881 ttgcccaaga agcaaaaaaa gatgacctcc ggcgagctgg ttgatttttg ggacaggctc
     2941 gttaaagact atcccatcgt gtcattggag gacggcctgg ccgaggagga ttggcagggc
     3001 tggaaattac tgactcaaag gcttggagat caagtgcagc tggtgggcga cgatttgttt
     3061 gtgaccaata ccgggcgtct cgaaaaagga atcagtctcg gcgttgcaaa ttccatcctg
     3121 atcaaactca atcagatcgg gacattgtcc gaaacactcg aagccatccg gaaagcccac
     3181 agggcgggat acacggcggt catctcccat cgttccggag aaacggaaga tactacgatt
     3241 gccgatctcg cggtagccgt gaacgcaggc cagataaaga cgggggcgcc gtcgcgcagt
     3301 gagcgggttg caaaatacaa ccgcctgctt caaatcgggc gggaactcgg ctctgccgcg
     3361 caatatccag gaatgtcggt attttcctga tttcagtaaa atgctggaga tatgctgaaa
     3421 ttgcttgggt tttaatgcga ttgcccggaa agccggtaat aataaacaac atattttatg
     3481 aacaggaagg tgttttaaat gaaagagatt ctgttttatc gctgtgagaa atgcggaaat
     3541 atcgtggcgc tccttaaaaa gggaggagga actctctcct gctgcggaca ggatatgacc
     3601 aggctcaagg agaattctac tgacgcagcg accgaaaaac atgttccgat tgtggcaaaa
     3661 gacggcggaa aagtgaaagt taccgtcggt tcggttgccc atccgatggc tcccgagcat
     3721 tttatcgaat ggatcgcact gtcctgcggt gaccggctgg agattgtctt cctgaagccg
     3781 ggcgatgagc cgaaagcgga atttacatgt ccggacggct cctcttgtgg gaccgcctac
     3841 gcctactgta acctgcatgg attatggaaa gctgattgta acgcttagca tgatgaagcc
     3901 gctgccggtt ccggcagcgg ctttttatag attgcttcat gtccggctat actaaaggta
     3961 gatcgcaatc gaatctcata ttactgctgc ggctgctttt tcttcgttct gtgttgccgt
     4021 ttgccggaat aaaccagtag ggcgaagcga taataattaa gtcttgacat tgtatacaaa
     4081 aatggtatac tatattcagg gaagaaattc cggaggtgag acacaatgag aacagacaga
     4141 agaaaagtcg tattagtagg caccggattt gtcggcatga gttatgccta cgcaatgctc
     4201 aatcaaaacg cctgcgacga gctcgtgctg gttgatattg accggaaacg agccgaaggt
     4261 gaagcgatgg acctaaacca cgggcttgcc ttttcgggat cacatatgaa aatttatgcg
     4321 ggcggttatg aagactgccg cgacgcggat attgtcgcca tagcggcggg cgtggcgcaa
     4381 agacccggtg aatcgaggct tgatctgctc caaagaaaca cgaaggtatt cggctcgatt
     4441 attaatccgg ttgtggagtc cggcttcaag gggattttcc tgatcgccac caatccggtc
     4501 gatattatgg cccatgtgac caaaaccatt tccggttttg accgaaaacg agtgataggc
     4561 acgggaacga cgctggatac cgcgaggctt cgatacatgc tcggtgaata ttttcaggtt
     4621 gacccgcgca atgttcacgc ttatgtcatg ggcgagcatg gagacagtga atttgtgccg
     4681 tggtcacagg cgctgattgc gaccaaaccg gcgctggagt tatgcggaca gtcgaacggc
     4741 cagtattcct gcgactgcat gtcccgcatc agcgcggaag ttaaaaacgc cgcgcagaag
     4801 atcattgaag caaaacaatc tacctattac ggcattggaa tgtcgatggt ccgaatctca
     4861 aaagcaattc tcggtgatga aaacagcgtg ctgacggttt caactcttct tgacggggaa
     4921 tatggccgca gtgatgttta tgtaggagtt cccgccatag tcagccgaaa cggcgtcgag
     4981 aaaatcatta cgctttctct gaccgacggc gagctgaaac agttccacga ttcatgcgat
     5041 cttctgattc agacttatca tggaatcaat tttgaattgc tttgagctga aacagaaaaa
     5101 tgactgggat agaaaaggag cggcaaatat gttaaataat gaaattgtaa caaaattgaa
     5161 cgagcagctg aataaagagt tatattccac ctatttttat ctggacatgt ctaattacta
     5221 ttttcatcag aacctcgacg gcttcggcaa ctggttttat gttcaggccc aggaggagcg
     5281 cgatcatgcg atgcttttca acaaatatct tctgaacaat gaccaggagg ttgttttgac
     5341 cgatgtaaag gcttccggcg tcagtttttc cgacttcaga gaaccgttga gcaaaacact
     5401 cgaacatgag cggtttatca ccgcatcgat caacgatatt tacgctgtcg cttatacaca
     5461 gaaggatttc cgcaccatgc agtttttaga ctggtttgta aaagagcagg gcgaggaaga
     5521 gaagaacgcc gatgatcttg tcaaaaagtt tgacctgttc ggcaaagaca gtcgagggct
     5581 ttacctgctt gacaccgagc tgaaagccag agtttatact cccgccaccc ttgttcttta
     5641 gcagaaagca gaaaccgccc cgaacgatcc gttcggggcg gttttatatg gcatgaaaat
     5701 tcaatttatc tgcgggcata actgagaaat aaagcgatat ttaaaactgt cacggtcagg
     5761 ccaatgcccc agagcagaac acgggtgaat cctgagtttg catatttccc catgactttt
     5821 ttggaggatg tcagatagat cagcgtaaaa attgtgaaag gaagctgcac gctcagcagc
     5881 atctgggaat agacaaggcc gtcaaacggc gaagtgatga agaaaataac tgcggcggcc
     5941 agaatcatcg tcatcaggac cccagttttc gtatggctgt cggaaatatc gtagggctcc
     6001 gaaaacactc ccgcataaat actgcctccc gccatgccgg cggtgacaca ggaggaaatg
     6061 ccggaaaata aaagagcaac cgcgaaaatg gcggcggcgg cattgccaag catcggtttc
     6121 agcagaagct gtgcctgcga aagctccgaa acctgcgctt tttgggtaaa gaaaacggtg
     6181 gccgcgatca ggatcatggc gctgttgatt gcccagccga tcatcatgga aaacaatgtg
     6241 tcgccaaatt caaaagccag ctgttttcga atgactgatt catcctgaag gttccactgc
     6301 cggctctgga tgatctccga atgtagaaaa aggttgtgag gcatgacaac tgccccgagt
     6361 acgctcatga taatgggcag ggatccgcct ggaatggccg gagtaatcca tccggatgcg
     6421 gcttttcccc agtcgaccgg aaccagcgtc agttcaaaaa taaaggatat tccgataatc
     6481 gaaacaaaac cgatgatcca cttttctatt ttacgatagg agttggtgaa cagcatccat
     6541 aaaacaaccg ccagaatgag aacggttccg agttttatgg gcaaatggaa cagcatattc
     6601 agggcgattg cgccgccgag cagttccgcc attgcagtgg aaacagccgc aagcattgct
     6661 gttgcgagaa tgaaattttt tagccagggg cgcagatgtt cggttgcggc ttccgaaagg
     6721 cacttcccgg ttacaatacc gaggtgggcg gagttatgct ggagcacaat cagcatgatg
     6781 gtcgacagag ttaccatcca cagcaggacg tagccatatt gggctccggc agaaatgttg
     6841 gacgcccagt tgcccgggtc aatgaatccc acggtgacaa ttagcccggg gccaatgtat
     6901 ttaaaaaaat ccaaagccgc cagccttggc ttatgggact ttttctgtgt tttcaagagg
     6961 ccaccgcttt ctcttcttcc atattctaat catagacgaa aagttcgggg atttcaaggc
     7021 ggtgtgtaac gttcattctc gttccatttc ttttcagtaa aggattcaaa cataaaaact
     7081 attgtatatt accaaagaaa tcatgtcaat tccacccggc gcggttgacg aattggtgat
     7141 ttggatggta acataattat tggtcataca tcgttaaaac ggcaggcctt taacgggtaa
     7201 aatggatttt tatcccatcg cagcttgggt atggcccgcc tatttgtcca tgaaggttcc
     7261 aaaattgacc gagaaccgaa atcctgatcg aaagaaagca taacgttttc atgccacggt
     7321 tcggcattat cacgtaaaag aaaatttaat caatcagtag aaaagaggga ttcgtatgga
     7381 tccggtcagc aaaatattga aaagctttcc ctgcatcgtt ttagacggcg ctctcgcgac
     7441 agaactggag cggagaggct gtgaaatcaa cgatgcgctt tggtcggcaa aaatactggc
     7501 ggaagatccc gaattaatcc agaaggtaca ttatgattac tttgtttccg gggcggattg
     7561 cgccattacc gccagctatc aggccaccat tcagggcttt atccgaaaag gcttttcagt
     7621 agaagaatca gaggctttga ttcggcgttc ggttatagtt gccaaaaaag cgagggatga
     7681 tttctggaag gatgaatcca accgtatgaa taggaccaaa ccctttgttg cgggttcgat
     7741 tggcccttat ggcgcctatt tggcggatgg ctctgaatac cggggtgatt accggatcag
     7801 tgaggacgag ctgatcgagt tccacaggcc cagaataagg ctgctgctgg atgagggtgt
     7861 ggatttactc gcctgtgaga caattccaag tctgatagag gcccaggcaa ttgtcaaact
     7921 gcttgaagaa ttctcggagg tttcctgctg ggtcagtttt agtgcaaaga atggttatga
     7981 aatcagcgac ggcactccga tcctggaatg cgccggatat ctcgaaacct gcggacaggt
     8041 tgccgcgatc ggagtgaact gcacaccccc gggatacatg aaatcgctga tcggtgaaat
     8101 aagaaagaat tcgaaaaaac cggtggtcgt ttacccgaat tccggtgaag aatatgatgc
     8161 ccggacgaaa acatggcacg gcagttccgg cggcggatcg ttcggctgct gcgcggaaga
     8221 ctggtttaaa gccggggcgc gtctgatcgg cgggtgctgc agaaccaggc cggaagatat
     8281 ccgggcaatc gcttcctggg ccagaaataa accgtaagac gctgtttcaa aagaacaccc
     8341 cccggaccgg tagagtccgg ggggtgttct ttagtttatc tttacgttcg ttaggatcgt
     8401 attcaagtgg aactcttttt tggctttctg tttgcgagaa tcatcagcgt aataatcgac
     8461 acaatgaaag cgattactcc agccgtaggt ttccactgat agaacagggc tgaaactccc
     8521 agagtaagca ggatactgct tagaattcgc aggaaactga gcggggcctg ccccttgacg
     8581 tctcccgttt gtccgttgat cataaaatga taggttttcc ctttaaaggt ataagcggag
     8641 atccagagag gcaacaggac ctgcttaaat cggacatcct ggtaatcggt ccgaaaatta
     8701 acgttgcgca cttcgtccgc ctctatactg tcggagatat tattgcggat ttcatcctcg
     8761 atgataccgc gtgctgtcgt ccagcattca tccttgctga tactgttgct ttccgccaca
     8821 aaaccgtaga tataatcctt ctgaaaaggc ctgagatcac gaagatcaaa ctgcattttg
     8881 atcagctttt gattgacgtg ctttgaggcg ttaacaagaa cgttgtcaaa agcatcatca
     8941 tgagttccgc ttaccgaata ccagcgggtt ttttggactt cctgcatttc ctgaacctgt
     9001 ttaccgtccc gctccaccgt taccgattcc gttacatagt agtgttcgcc ggcttcggcg
     9061 gtgtaatcgg aggaggttgt gcagtcatag gcgaaatggg ggatataggc gccctgaagc
     9121 cggccgggct gattctgacg tttgagtttg cccggtgaaa aaaatcttcc cctgatccag
     9181 tctttgaaaa gcgaggatgc cgctttttga gaaattcgga aaggaacgat tccatccggc
     9241 accatcgctt tggaattctc cgccttagcc atcatcgggg agccgcagaa tgtgcaaaaa
     9301 tccgccgaag tgttcgggcc gaccacgata ttggctccgc agttgttgca gtggagcagg
     9361 acaccggggt cttctttttg ctcaagttct tcgccggtat aggcgaaatc atggcagttg
     9421 atatcctgac tgcttataat ttcctgattc ttaccgcagt aagggcagtt tagcgcgcct
     9481 gaatctggat cgaaggccat ttctccgccg caggatgggc atttggcatg ttccgtatcc
     9541 tgtcccgcgg tatgtaattc ctcgcttgtt gccatgtttt tcacctcatt ctaccttagt
     9601 gccgcagtcg gggcagaatt tcgcgcccgg ttccagctgt ttgctgcaat ttttgcatac
     9661 gggattgaca ttttgcgcgg cgccgcattc cgaacagaat tttgagccgt ccttgagcgg
     9721 agctccgcat ttgacgcatt ttactccggc gggcacaatc ggtttgccgc attccgggca
     9781 aaattttgta cctgccgtaa cctgtgcgcc gcaagaaggg catttgacaa aagtctcaac
     9841 cgacttctgt gaaaccacgg tctgggccgg ctggttaaag gcctgcataa acgccgaacc
     9901 cataaccgcg ccggcgccaa gaccgatccc agcgcccgca agaccgccgg gattcttcgc
     9961 cgcgtccggg atagagttcg ccgcctgaaa ctgagcgtac tgctgcaggt tgcctaacat
    10021 gcccatttgg gttcttgtgt caagggtctt ttccacttct tccggcagag agatattctc
    10081 gacataaaaa gaaacgagtg taaggcccat agcctcgaat ttcgggctca gtttggcctg
    10141 tacctgttcg ctgatttcat catagttgga tgccagatcc aaagcgggta tttttaattc
    10201 ccccagcaga tcggtcagcc ccgacacaat tacccgtttg agttggccgg taatgctttg
    10261 tgtgtcaaaa agtccgttgg tcccggatat ttgctgtaaa aagaccagag gattactgat
    10321 acgaaaagaa tagatgccga agccccggag tcttagcatt ccgaattcgg cgtcgcgaag
    10381 catgatggga ttctccgtgc cccatttttg gtcggtgtat tgccgggtat taaaaaaata
    10441 aacctcggct ttaaaagggg agttaaaccc gtaaggccat gatctcaatt tggtcagaat
    10501 ggggaggttg tttgtttcca gcgtataaag gccgggagaa aatacgtcgg cggccttacc
    10561 ttcatttaca aagatggcca gctgtgactc cctgaccgta agttttgcgc ccattttaat
    10621 ttccttgttt tccacgggaa aacggtaaac catcgtgtcc tgtgaatcat caacccattc
    10681 aatgacttct ataaactggc tttttacaaa atttaagatt cccaaataat catctccaca
    10741 gataaattga cccggaaagg ccatttaata aagaaactga cggatgctgc tgtcaaaatt
    10801 ataatattta aaccaatcat tgtaaatgcc tttttacgaa atcggcggta taatctgcga
    10861 aaggttttct gtcgcaaatg atcgttccgt gttgtttttt gactggcagg ggtatcgcct
    10921 ggcgagaaaa atccgataag catccccgtc tcgattcgtc tgaatcagcc gattctgttc
    10981 tcaatatcgg taaaaataat gttgatcagc tggtctgtca gcatctttgc atcttcgccg
    11041 ttctcatatt tgcggatgat cagggaaaac ccctcgacca gagaagttga gagcacggtt
    11101 gcaagatacc cgtcccgtat gacgatgcct ttcttttccg ccggaatcag tagttgatgc
    11161 gtaagaatat cactgataat cgaaatcata tccgatttta cattttcaaa tttagagcct
    11221 ctgcttttgt tcatcaggat aaagagctcg gtgttatggt ccttgcagat ttccagcaga
    11281 tcttccttaa atgcgttgat ttctctgagc tgcacgggtc ctgcgggagc gttcggatcg
    11341 gagggggcgg ttggaacgtt cctgattttg gcgatgaatt catagatgtt atcgcacacg
    11401 ggacccacaa gagaatcaaa aagatcttct ttgtttttaa aataccggta taaattgccc
    11461 agcgcggcgc ccgcattttg agaaattcgg cgcatggatg cgctgctgta acccttttct
    11521 ttaaattcgt cccgggcagc agtgataatc ctatttttga catcgtattt taaatactgc
    11581 atcaaagagc ctccgtccgc gtcttaagta taaattgtgt ccgtcctgaa aggaagaaat
    11641 tgaacatggc tatcccagcg ctaagaccag acggggaacg atctgctttt ttcttgatac
    11701 aactccgctc atcaaaaaac tgttttcatc ttgcaacgga gaaaaagccc tcgccacaag
    11761 ccctttttgt gactcaacaa aaaggacttc tgtctcttct tcgagaatat cggtaaagag
    11821 aatcaggaga attccaaaat gttctctggc agccagatcg tttaaacagg tcaggataag
    11881 ctgccgtaac ttttttaaac tgctcagatc ggttgtgttg acctggccga ttccgatttt
    11941 atgttttccg atttgatatt ccttgaaatc gttttgaatc atttcttcgg ttgtgagatt
    12001 tttcagcgaa gatcctgctt caaacatttt tgcgctgaaa tcctccatgt caatgcccgc
    12061 gagtttttcc agccgctcgg cggtgataag gtctgcgtgc gtactggttg gggatttgaa
    12121 atggatcgtg tcggatagaa tggcggcgca aagaataccg gcaatttggg gggaaggttt
    12181 gactccctgc tcggaataca gattggctat aatcgttgcc gtgcttccgg tcggttcatt
    12241 tcggtaaaaa atcgggctgc ccgtctggat gtctccaatt cggtgatggt cgatgatttc
    12301 cagaatttcg gcctgttcaa tgccgttgac cgactgggct ttctcgctgt gatccaccag
    12361 gatcagcttt tttttacgcg gattgagcaa gtggtaacgg gaaataaagc ccttgaagtg
    12421 ccggttttca tccacgaccg ggtagcttct gaatcgtgtc tgcagtaatt tttcccttac
    12481 gtcgtcaatg aagtcgtcga tatggaataa aaccagattt tcagaagtca tggtgaaccc
    12541 tacggggatg ctctgataga tcagccgcgc gcctgtgaac gtgtcatgct ttgtaatgag
    12601 aagggtgcac tgatgggcct cagccagccg gaccacatct tcggaaaccc tgctgccgca
    12661 ggttacaatc acacagctcg cgcctgcttc aatcgccttt atttgattga ctttaatgtt
    12721 gccggtaaag acaatgtcgc ctttttcaat aaactcgtcc attccatcgg gcgtcattgc
    12781 tgcgatgatt acttttcctg aatcgtccag tgattttccg aaaacgcaga ttggctcggc
    12841 gtccaatgtc cggatcaggt ttccgatggg agtgctgctg tcagacagcg cgttgttatc
    12901 cggcatgttc atatattcat ttgcaatatc cgagacggaa acgatcccct gtaaaatctg
    12961 ccggtcatct tcgattggaa gaacttccag attgcttgcc ttgagttttt tccatgcctg
    13021 ctgaattgaa gtgtcgggca ggacaggttc gatcctatcc atgttcaggt cagatacctg
    13081 ggtttttacc gttgcaagat attccggggc gtccagacca aagtaattca gcacaaattt
    13141 agtttcccgg ttgatttttc cggttcgaac cgggatggcg tcaatatgga gcattcgttt
    13201 aaaatcggca taggcgatcg cggaacagat cgaatcggta tccggatttt tgtggccggt
    13261 aatataaacg gcatggcttt tttctgaatt gtctgtgttc attaagaacc acctcataac
    13321 tcaatcataa tcaaaaatat gtgcatgcgc aatagcagac ggtgtcattt ggagcttcgt
    13381 gaaaaggttt tctgcaaagt tgttcagaaa ctggattgtt tgcacatcct gatccgggaa
    13441 aggcgaggct tttatcacga agccttatgc ccgaaaggag tagtttatca gatgatggac
    13501 ggccaacgca cttttgacga tttccgatta actccactct ttttcctgaa attatcaaaa
    13561 ttaccctaat atatttcata gatctaacga taaataaaga gaggtaagtt aatttgaaaa
    13621 ggagatcttt taatgtctgt caatcctata ttgtcaaccg ggtcattaac ttcattgata
    13681 accatgcagt catctctgaa aacggcaagc gcggtgaaag gcgctcagaa tgagctgcaa
    13741 acggaggctc ttatctataa gcccagcttc ggatttcagg gcgatccgaa aaaagcggca
    13801 gagctgtcat cgaaggcgga tagcctgaat ggtaaaattg gggagatttg ctcagacgtt
    13861 caaaaagaaa ttcagaagac aaacgatcag aatgcagaca gcagcaccgt tcaaacgcag
    13921 acggattcgc agagtactgc ggataagacc gatcctgcag aaacggtcgc ttctgcaggc
    13981 gggcagttat ccgtaagtcc gtcggaacaa aatacgggtg ataagctgct ttcaacaggt
    14041 aaaaatgtgg atgtaaacgc ctgattttaa agataatgga gcaatgttaa agaagctgcc
    14101 tgcaaaagag gcagcttctt tttatcggac attctgagaa tttcattttt attgaaccgg
    14161 ttcagaaagt ttcttgcaat aaaactccaa acgcccgatt ccctgttccg aataggcaat
    14221 ctcgcttaca tcaaagtggg cgtcttcagc gcattttatc atcacatctt tttcaaaaaa
    14281 ggtcaaatga gccggttcgt accaccatga tgtatattgt cggttaaata cactgtcttt
    14341 tatgggcgta gccagcacca ttatgccgcc ggg
//
LOCUS       DATALY010000168         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_641983,
            whole genome shotgun sequence.
ACCESSION   DATALY010000168 DATALY010000000
VERSION     DATALY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641983"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VHR42_04905"
     CDS             <1..1429
                     /locus_tag="VHR42_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX3026547.1"
                     /translation="GGQKKRVAMAGALIRPSGLLILDEPTNHIDNFTVDWLEQYLNRR
                     KGALLMVTHDRYFLERVANRILELDHGRLFSYAENYSKYLERKLEREESETASNEKRK
                     NLLRTELKWIQRGARARSTKQKARIDRFEKLKEEKAPSTYSGIEISVLSSRLGKKIVE
                     IEHLSKGFGDRKLIDDFSYRLLRNDRVGIIGPNGSGKTTLLNIIAGITPADSGDVRIG
                     ETVKIGYFSQDCAEMDGSLRVIEYMRNIAEYVTTDEGSVSASQMLERFLFPPDMQWTP
                     ISKLSGGEKRRLFLLSVLIAAPNVLLLDEPTNDLDIQTLTILEDYLSRFPGAVIAVSH
                     DRFFLDRVVKKILFYDNGSIRQISGAYSDNMDQFDKTPSSGDSAKSNGGPQKPNRTER
                     QTKKTLKLTYKEQRELEGIDSLIAGLEENIKKCKNKMQLSSSDYTKLEELLAKQHSLE
                     SELDKAMERWVYLNELKEKIESEKK"
     gene            1486..2643
                     /locus_tag="VHR42_04910"
     CDS             1486..2643
                     /locus_tag="VHR42_04910"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3026548.1"
                     /translation="MNNFDFVSLPLRGALQKEQITVPTQVQETVIPLALQDKDLIVQS
                     QTGTGKTLAFLLPLFERLNPAGRETQAIILAPTHELAIQIQRQIERLALNSGIGLRSA
                     PIIGNVNILRQIEKLKSKPQIIVGSPGRILELIKKRKISAHTVKTIIVDEADKMLDKN
                     NIDSVRAVINTTLRDRQLLFFSASISSSTTAGAKLLMKQPELIQLGVDRSIPSSIEHC
                     YFFAEERDKIEELSKLAKIIHPEKAMVFINREFDNDIAVEKLKYRGLSAGSLYGGIHK
                     FERKTALENFKTGKLQYLVASDIAARGLQIEGISCVFNISMPEDPLDYLHRAGRTGRN
                     GMQGLSISIITPRELALIHLYEKKFNVPFHAKKMFKGKIEDLKQRGSGKTR"
     gene            2753..>2996
                     /locus_tag="VHR42_04915"
     CDS             2753..>2996
                     /locus_tag="VHR42_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3026549.1"
                     /translation="MGTFFDLINQRESCRNYADKPVEKEKLIACVEAARIAPSACNSQ
                     PWSYVVVNNRVLSSKVAKCLQGAGMNKFTDQCPAFIV"
BASE COUNT          865 a          712 c          752 g          667 t
ORIGIN      
        1 gggcggtcag aaaaaacgcg tcgcgatggc gggcgcgctg attcgcccat ccggcctgct
       61 cattttggac gagccgacaa accatattga taactttacg gtggactggc tggaacaata
      121 tctgaatcgg cgaaaaggcg ctctcctgat ggtcacccat gaccgttatt tccttgagag
      181 agtggcaaac agaattctgg agcttgatca cgggcgtctt ttcagttacg cggaaaacta
      241 cagcaagtat cttgagcgga agcttgaacg ggaagaaagt gaaacggctt cgaatgaaaa
      301 gagaaaaaat cttctgcgca ccgagcttaa atggatacaa aggggcgcca gagcccgctc
      361 aaccaaacaa aaagcaagga ttgaccgctt tgaaaaactg aaggaggaaa aagcgccttc
      421 aacctacagc ggtatcgaaa tcagcgtttt gtcctcccga cttggcaaaa aaatcgtaga
      481 gattgaacac ctgagcaaag gcttcggcga ccgaaagttg attgacgatt tcagttaccg
      541 ccttttgcga aacgaccggg tcggcattat cgggccgaat ggcagcggaa agacaacgct
      601 tcttaatatt atcgcgggca taacgcccgc cgacagcggc gatgttcgta tcggcgagac
      661 cgttaaaatc ggttattttt cacaggactg cgccgaaatg gacggcagcc tgcgggtgat
      721 tgaatatatg cgcaatatcg ccgagtatgt gacgaccgac gaaggcagcg tcagtgcctc
      781 gcagatgctc gaacggtttt tgttcccgcc ggacatgcag tggacgccca tatccaaact
      841 ctccgggggc gaaaagcgaa gattgtttct acttagtgtt ctgatagccg cacctaatgt
      901 gctgctgctc gacgaaccga ctaacgatct ggatattcag acgcttacaa ttcttgaaga
      961 ctatttgagc cgatttcccg gcgcggtaat cgccgtatcg catgatcgct tttttttaga
     1021 ccgtgtcgtg aaaaaaatcc tgttttatga taacggcagc atccgccaaa tcagcggcgc
     1081 ctattccgac aacatggatc aatttgataa aacaccctcg tccggcgatt cggccaaaag
     1141 caacggcggt cctcaaaagc caaaccggac ggaaaggcag accaaaaaaa ccttgaagct
     1201 cacctacaag gagcagcggg aactcgaggg catcgacagc ctgattgccg ggctggaaga
     1261 aaacatcaaa aaatgcaaga ataaaatgca gctttcgtcg tctgattata cgaagcttga
     1321 ggagctgctt gcaaagcagc attcgctgga gagcgagctt gacaaggcca tggaacgctg
     1381 ggtgtattta aatgaattaa aggaaaaaat agaatcggaa aagaaataag acagcaggat
     1441 caattccatc tatgatgaca ttttaaccaa tttgccggag gtactatgaa caattttgat
     1501 ttcgtcagtc tgccgcttcg cggcgcgctg caaaaagagc agatcaccgt tccaacgcag
     1561 gtgcaggaaa ctgtcattcc gcttgcgctt caggacaaag acctgattgt tcagtcacaa
     1621 acaggcacgg ggaaaacact ggcctttctg cttccgctgt ttgaaaggct gaatcccgcc
     1681 gggagggaaa cgcaggcaat tattctggcg cctacgcatg agctcgccat ccagattcag
     1741 cgccagattg aacggctggc cttaaactcg ggcattgggc tgcgttccgc gcccattatt
     1801 ggcaacgtca atattctccg gcagatcgag aagctcaaat cgaaaccgca aattattgta
     1861 ggctcaccgg ggcggatact ggaactgatc aaaaaaagaa aaatttccgc ccataccgta
     1921 aaaacgatca ttgtggatga agcggataag atgttggata aaaacaatat tgacagtgtg
     1981 cgcgcggtta tcaataccac cctgagggac agacagcttc tgtttttctc cgcgtcaatt
     2041 tcatccagta caactgcggg cgcgaaactg ctgatgaagc agcccgagct catccagctc
     2101 ggcgttgacc gctccatccc ctcctcgata gagcattgtt atttcttcgc ggaagaacgg
     2161 gataaaattg aagaactttc caagcttgcg aaaatcattc atcctgaaaa agcaatggtt
     2221 ttcatcaacc gggaatttga caacgacata gcggtggaaa aactaaaata tcgcgggctt
     2281 tccgccggta gcctttatgg cggaattcat aaattcgaga gaaaaaccgc tctggaaaat
     2341 ttcaagaccg gaaagcttca atatctcgtt gcatccgaca ttgcggcgag aggccttcaa
     2401 atagagggaa tttcctgcgt gtttaacatc agcatgccgg aagatcctct cgattatctg
     2461 caccgggccg ggcgaaccgg cagaaacggc atgcagggtc tctctatttc catcataacg
     2521 ccgagagagc tggcgctgat tcatctgtat gagaaaaaat tcaacgttcc gtttcatgcc
     2581 aaaaaaatgt tcaagggcaa aatcgaagat ctgaaacaac gcgggtcagg aaaaacccga
     2641 taaattaacg gtttcgggcc gtcttaatca cacctggata aaaacgcatg tttcatgcga
     2701 tcatttctat tgaggatagt tgaagaaaaa gtatcgaagg gagtgctgta tcatgggaac
     2761 ctttttcgat cttataaacc agcgtgaaag ctgccgcaat tatgccgaca agccggttga
     2821 aaaggaaaag cttatcgcct gcgttgaagc ggcgagaatc gctccgtcgg cctgcaacag
     2881 ccagccatgg agttatgttg tggtaaataa cagggtgctg tcttccaaag tggccaaatg
     2941 ccttcagggc gccggtatga ataaattcac cgaccaatgc ccggcattta tcgttg
//
LOCUS       DATALY010000169         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_37090,
            whole genome shotgun sequence.
ACCESSION   DATALY010000169 DATALY010000000
VERSION     DATALY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37090"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /gene="whiA"
                     /locus_tag="VHR42_04920"
     CDS             <1..199
                     /gene="whiA"
                     /locus_tag="VHR42_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX3026550.1"
                     /translation="HCADEDVRNYPFSLPEELREIAMMRLENPDATLRELGGMLRVRL
                     SRSGVNHRLKKIIGIAEKIRG"
     gene            253..>1529
                     /gene="dnaE"
                     /locus_tag="VHR42_04925"
     CDS             253..>1529
                     /gene="dnaE"
                     /locus_tag="VHR42_04925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX3026551.1"
                     /translation="MDNFVHLHLHSEYSLLDGACRIGDIIRRAKELGQPAVAITDHGV
                     MYGVVDFYKQAKKEGVKPIVGCEVYTSARTRHDKIHHLDSTHGHLVLLCKNNEGYRNL
                     AKLVSEAWIDGFYGKPRVDMELLRQYCGGLIALSACLSGDVPKRLLAGDYEGALSKAL
                     EYRELFGEDFYLELQDHGLSEQKTINPQLIKLSHQTGIPLVATNDVHYLTKPDAWLQK
                     VLMCIQMNRTVDDPGHAGFSTDDFYMKSTEEMKALFPNIPQAFDNTLKIAEECNVEFE
                     FGRTILPHFEVPEGRDHFEYFKDLCEKGLEERYGEKIEENVRKRLDYELDIIQRMGYV
                     DYYLIVYDFIRYAKSVDIPVGPGRGSGAGSLAAYAVGITGIDPMKYNLLFERFLNPER
                     ISMPDFDIDFCYERRQEVIDYVIRKYGSDHVSQT"
BASE COUNT          390 a          365 c          416 g          358 t
ORIGIN      
        1 tcactgtgca gatgaagatg tacgaaatta tccattttct ttgccggagg aactgaggga
       61 aatcgccatg atgcggctcg agaacccgga tgcaaccttg cgtgagcttg gcggaatgct
      121 ccgggtcaga ctcagccgtt ccggggtcaa ccaccggctg aaaaaaatca tcgggattgc
      181 ggaaaaaata cgcggttagg gccataggct ccgccgctta aaatcttaga ttcaggaggg
      241 ccagcaaagt aaatggataa tttcgtacat cttcatctgc acagtgagta cagccttctc
      301 gacggagcct gccggatcgg ggatattatc cgaagggcga aggaactggg gcagcccgcg
      361 gtcgcaataa ccgaccacgg cgtgatgtac ggtgtggtcg acttttataa gcaggcaaaa
      421 aaagagggtg ttaagccgat tgtcggctgc gaggtctata cctctgcccg aacgcgccac
      481 gacaaaattc atcatcttga ttcaacgcat ggccatcttg tgcttctctg caaaaacaat
      541 gagggctatc gcaatcttgc caaactcgtt tccgaggcgt ggattgacgg gttttacgga
      601 aaaccccgag tcgatatgga actgctgcgt caatactgcg gaggcctgat cgcactttcg
      661 gcctgccttt ccggcgacgt tcccaaacgg ctgctcgccg gtgattacga gggcgcgctg
      721 tcgaaagcgc tggaatacag ggaactgttc ggagaagact tttatcttga attgcaggat
      781 cacggtctga gcgaacagaa gacgatcaat ccgcagctga ttaagctgtc tcatcagacg
      841 ggaatccccc ttgtggcgac caacgacgtt cactatctga ccaaaccgga cgcatggctg
      901 cagaaggtgc tgatgtgtat ccagatgaac cggacggtgg acgaccccgg ccacgccgga
      961 ttttccaccg atgattttta tatgaaatcc actgaggaaa tgaaggcgct gtttcccaat
     1021 atccctcagg cgtttgacaa taccctgaaa atcgccgaag agtgcaacgt cgagtttgag
     1081 tttggccgca ccatccttcc gcattttgaa gtgccggaag gccgcgatca ttttgaatat
     1141 tttaaagatc tgtgcgaaaa gggtcttgag gaaagatacg gggagaagat cgaagaaaac
     1201 gtccggaaac gtctcgatta cgagttggac atcatccaaa ggatgggtta tgtcgactat
     1261 tatctgattg tctatgattt tatccgttac gcgaaaagcg ttgatattcc cgtcgggccc
     1321 ggcagaggct cgggggccgg aagtcttgcg gcctacgccg tcggtattac cggaatcgat
     1381 ccgatgaaat acaacctgct ttttgaacgg tttcttaatc cggaacggat cagcatgcct
     1441 gactttgata ttgacttctg ttatgagaga aggcaggaag tcattgatta tgtgatccgc
     1501 aaatatggca gcgaccatgt ttcacagac
//
LOCUS       DATALY010000170        16880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_298420,
            whole genome shotgun sequence.
ACCESSION   DATALY010000170 DATALY010000000
VERSION     DATALY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16880
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298420"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VHR42_04930"
     CDS             <1..777
                     /locus_tag="VHR42_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026552.1"
                     /translation="ASKTFLSSLTNTAVISAGKMITVPAGAYYVRFAAASSAGAFQLE
                     LGSTATTYEAYKSSILSITGTVTASDRFTWDGITGKIDGSAVVTSGNLQAYQNGWVFV
                     QNSDGTPVTVVPATEVTYNLLGDMRISAFANGSGSANTNKVDHAVFAEVGTTRTQGDN
                     SSKLSTTAYVDSGLSGKMPMSNNTSGIVGYFTKISGTVGAAYSLPTGGTWEYFGFLVN
                     SSTGAIFGPTGFFAGVSAGGTQVAAATSGYYPAGYIRRIA"
     gene            complement(801..1817)
                     /locus_tag="VHR42_04935"
     CDS             complement(801..1817)
                     /locus_tag="VHR42_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX3026553.1"
                     /translation="MFDGKTLLITGGTGSFGNAVLKRFLETGIREIRIFSRDEKKQDD
                     MRKLYHNKKISYYIGDVRDIASVRNSMHGVDYIFHAAALKQVPSCEFFPLEAVKTNVL
                     GTDNVLTSAIESGVQKIVCLSTDKAAYPINAMGISKAMMEKVFMAKSKTIDPDKTLIC
                     GTRYGNVMASRGSVIPLFVSQIKSGQPLTITDPAMTRFLMSLDEAVELVVFAFEHAES
                     GDLIVQKSRAASIGDLALAVKQLFDADNPIKIIGTRHGEKQYETLLTREEMVRAIDYG
                     EFYRVPIDKRDLNYEKYFTSGNMKIGLSSEYTSNNAIRLSTVEITEKLLSLEYIRQQL
                     KDRS"
     gene            complement(2024..2911)
                     /locus_tag="VHR42_04940"
     CDS             complement(2024..2911)
                     /locus_tag="VHR42_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3026554.1"
                     /translation="MKILCLGDNNVDIYEDLGVVFPGGNTYNVAVYTAMNGCRSAWLG
                     AVGNDQNGRLQIDALKHFDIDASRHRVYQGQTAWAKVSIVNGDRIFMENYLEDEKQNP
                     IILDSSDMEYIRSFDLVHSSAYSYLKADTLKKVRETGRKISYDFSVEWNEEVLRTICP
                     HLDFGFLSLGSRKESEARDILYLAYNCGCDIVVGTMGKRGSLLYDGKNFLRQKAYLVP
                     SRDELGAGDSFISKFLQCCLDGMLFRKSTEREAGITFESSDMDDYQEKLLCHSLSLAA
                     VFSARTCMTSGAFDYSVKL"
     gene            complement(2935..3936)
                     /locus_tag="VHR42_04945"
     CDS             complement(2935..3936)
                     /locus_tag="VHR42_04945"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3026555.1"
                     /translation="MANCTVTSHEEIKKTVANLLSRKKTDGGIKSIYFVACGGSKAGL
                     NPGAYFLQHESSNLIVDNITANEFVHDVPRNVGKNTIVVTMSQMGNTKETVAAAELAK
                     KKGAASITISVRNDVPIAKFSDAHLLYDEDLYTDVLNGCMVLTLRLGIELLNQIEGYK
                     NYDLALESFEKLGMITEKAVEMVSERAREFAEECKAEPVIYTMGSGPSHYAAYMNCIC
                     LLMEMEWIHSNCIHSGEFFHGPFEITDMRTPFIVFENEGKTRVLDDRAIKFLKTYGKK
                     IYIIDSKEFGINIIDDAVIDYFNGPLLWSVSLVYMKELAAAKRHPLMMRRYMYKVEY"
     gene            4127..4891
                     /locus_tag="VHR42_04950"
     CDS             4127..4891
                     /locus_tag="VHR42_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3026556.1"
                     /translation="MGNRMNNSERPLSGLKAVPLYKQLKAMLLEQLENQVWKNNEKIP
                     SEAEISGIYGVSRVTVRSALNELVEDGYLIKIQGKGTYACHTIKKITLSADSGSFHQF
                     CTKEGLVPGRRVLVCESVEANQEDVEELRLSPGDRVVHLYSVMTVEGVPITLEDNRLS
                     SRFDFLMELDLTDQSIWQLLMTHGDVRKIRVASHVVDSSLSNSKESQLLQVPKGTPML
                     IINELNCDENGDPVHRTTLKSPSNRVKFYYDWGNMG"
     gene            complement(4940..5569)
                     /locus_tag="VHR42_04955"
     CDS             complement(4940..5569)
                     /locus_tag="VHR42_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3026557.1"
                     /translation="MVKSESLRNIVYNYLVQKIQGGELKPNDKINESTICNELSLSRT
                     PTREALIKLEADGLLMYTPNKGFTVKEVSEKDRDDIYIIIGSLDSLAATLAMSQITEA
                     DIIRMYELTDKMDIAIKYANFPDYAEFQTLFHNVYTDKCKNRFLIDILVDLRKTFVPQ
                     AYLTEMKPDLFDHLNAANDEHRQIIRLFEKKDLPALQHFLKEKHWQNIL"
     gene            5759..6430
                     /locus_tag="VHR42_04960"
     CDS             5759..6430
                     /locus_tag="VHR42_04960"
                     /inference="COORDINATES: protein motif:HMM:NF027487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HEX3026558.1"
                     /translation="MTIDEIRALNGNYKIFSVTDPEFKAYGKVVEGLNFSSMIDFAEK
                     NLEIPKDGNKYVASVKELEAFPVMKEVEYDIYGEMPIEAGFCTGQNTALTGYERHQGS
                     EVNVAVTDCILILGKQQDMDGQTFDGAKSEIFYVPKNTAVELYATTLHYSPCKVCNDG
                     FMVIVILLEGTNSAFAADHKPAGTNKLLTKKNKFLIVHPSQTAKIQAGTLPGLKGDMI
                     DIKVK"
     gene            6631..7473
                     /gene="larE"
                     /locus_tag="VHR42_04965"
     CDS             6631..7473
                     /gene="larE"
                     /locus_tag="VHR42_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX3026559.1"
                     /translation="MNQEEKLEKLKNDLKSMKRLIVAYSGGVDSTFLLRVAQDVLHDG
                     VIAVTARSSTYPEREFNQSKELAGKIGARHFIIESEELDIDGYSSNPVNRCYYCKKEL
                     FTKINELASRMGVQYVADGSNVDDLCDYRPGRQASGELNVVSPLKDAEMNKQDIRELS
                     RKMGLETWDKPAFACLASRFPYGQQITHEKLKMVDEAEQYLIDRGFRQVRVRHHGEIA
                     RIEVAVEERPKFFDEKLMDAVHEKFHKIGFQYIALDLRGYRMGSLNEGMVNQSASRKA
                     GAEQ"
     gene            7494..8627
                     /locus_tag="VHR42_04970"
     CDS             7494..8627
                     /locus_tag="VHR42_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3026560.1"
                     /translation="MQYRKFGKLDVKVSSLGFGTMRLPLNSNVDSDINEPEAIRMLRH
                     AIDNGVNYIDTAYPYHQGKSENLVAKALKDGYREKVLLATKLPVWLVKEYSDFDKYLN
                     EQLKKLETGQIDFYLLHSLSKKEWKKMIDLDVFKALENYKKSGKVKYIGFSFHDDFET
                     FREIIDAYDWDFCQIQYNYMDEHEQAGTEGLRYAASKNIGVVVMEPIRGGHLSANPPD
                     QVKEIWNSAPVKRSPAEWALRWVWNHPEVSVVLSGMSNIEQVEENIRVASAAEPESMT
                     SEELTIVGKVRDTYRGLTKISCTGCRYCMPCPFGVSIPGNFNIYNDAFVYNILESKRK
                     EYQGRESSKLAANCHDCGKCEEACPQHLPIRKLLKDVDAALAR"
     gene            complement(8703..8864)
                     /locus_tag="VHR42_04975"
     CDS             complement(8703..8864)
                     /locus_tag="VHR42_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HEX3026561.1"
                     /translation="MSKHSIMSEKLKYEIAKELGVDDKVKREGWGSVSSRDCGNIVKK
                     AIEIAERSV"
     gene            complement(8964..9671)
                     /locus_tag="VHR42_04980"
     CDS             complement(8964..9671)
                     /locus_tag="VHR42_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX3026562.1"
                     /translation="MNKKGTVPFNGFSQKTLDFLFENRLKNSKTWFEEHRGEYETYVL
                     EPLRALVVNLTPVMLSIDPLFTVEPAVNKTIARIYRDVRFSRDKSLFRDEMWITFMRN
                     KRFWAGLPGYYFLLGQGGFSYGFGYYEATTESMAIFRDCIMKNDRAFQKALAAYQNQT
                     EFQLNGEKYKRSKFPGQPEEVMDWLDRKNISFDHHSNDLSTLFSPRLADELSTGFQQL
                     KPIYDFIFLVEQKRRRD"
     gene            9814..10752
                     /locus_tag="VHR42_04985"
     CDS             9814..10752
                     /locus_tag="VHR42_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX3026563.1"
                     /translation="MVFLHAVGSILTVVLMFMTGYFMTAAGWLDDRTSDTFSRIILNV
                     SLPCYMLYNIVSNFDRARLEQLSGGLIVPVLSIGITYLIGVAASNLLHVGEGRKGIFR
                     SVFFTSNTIFIGLTVNLSLFGEKSTPYVLLYYIVNTTFFWTIGNYEISRDGPGAHSQA
                     FFSRSTLKKIVSPALGGFLVALILVLIGIKLPSFILDACRDIGGMTTPLAMLFIGIVL
                     RSVKLRELKLDREILVLVAARFLICPLTVIALEHFIKLPPLMAKVFVIQSAMPAMTST
                     GVVAKGYGADYEFGTVVTVITTIMGMIAIPIYMCVI"
     gene            10840..11292
                     /locus_tag="VHR42_04990"
     CDS             10840..11292
                     /locus_tag="VHR42_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX3026564.1"
                     /translation="MLGAQAIGNEPSIILLGRINAAACFLKRHPNSVCIAAGGQGSDE
                     TESEAKCIKDYLTSRYGIEAKRIYTDDQSKSTSQNFDNAIKIIKRYNLSDNVAVATDG
                     FHMFRAKLLAQRKGLNAYACPAVTDKRLVLTFYIRELFALPKTVLFNQ"
     gene            complement(11353..12063)
                     /locus_tag="VHR42_04995"
     CDS             complement(11353..12063)
                     /locus_tag="VHR42_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEX3026565.1"
                     /translation="MIDPASLLMADLYPVSTGAASGSNGSGKEISAIPFDQMLSEAIE
                     NTKDLPKGPSFDEYLSSILALGFYSTPVVSSTGVSGSSGNMQVSDSLVNFIASKEGYS
                     ATAYRGVDSQNQTIGYGHVIQPGENYTYLSKSDALKLLKSDIQKYSNSVNQEFSGTSL
                     SQNQFDSLVSFSYNLGANIWDSVPKLTSDIKSGASADTLREDFARCCHCNGEVVSGLY
                     NRRISEWKMFCYGNYYST"
     gene            complement(12346..12480)
                     /locus_tag="VHR42_05000"
     CDS             complement(12346..12480)
                     /locus_tag="VHR42_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026566.1"
                     /translation="MPKDSQPDRKETRKRKSNSQTPLTRENQNRSRTAKKQSAEHNDV
                     "
     gene            complement(12504..12743)
                     /locus_tag="VHR42_05005"
     CDS             complement(12504..12743)
                     /locus_tag="VHR42_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX3026567.1"
                     /translation="MSKQSVYYSLDDIVTRHDAKALKKGIDQISGVISVSVNTHDKKI
                     AVDYDSTGTNGPEIMDKISRLGFKASLTEHEDHIM"
     gene            complement(12852..13343)
                     /locus_tag="VHR42_05010"
     CDS             complement(12852..13343)
                     /locus_tag="VHR42_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEX3026568.1"
                     /translation="MQRRDKQNYYLDIAQTVLERGTCLRRNFGAIIVSNDQIIATGFT
                     GAPRGRKNCSDLGTCMREKLKIPRGERYELCRSVHAEANAIIAAARNDMIGSTLYLVG
                     QDAATGELVKGANSCAMCKRMIINAGIAKVIIRCTHDDYTIIDVKREWVEHDESLENS
                     MGY"
     gene            13607..14470
                     /gene="rsmA"
                     /locus_tag="VHR42_05015"
     CDS             13607..14470
                     /gene="rsmA"
                     /locus_tag="VHR42_05015"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX3026569.1"
                     /translation="MNHLTDAAYVRKLLESYGFNFSKALGQNFLINPSVCPKMAELSG
                     AGKGVGAIEIGPGVGVLTAELAERAEKVVAIELDSRLIPVLRRTLKDYDNIRLINGDA
                     LETDLKEIVTNDFAGLEAVVCANLPYYITSPVIMHLLESKLPVNSITVMVQKEAGRRI
                     CAVPGSREAGAVSLAVRYYSEPAILFDVSSGSFMPRPKVDSCVIRLDVRSSPPVDVKN
                     EKLLFRIIRAAFNQRRKTLLNGLSAGLGQSKERTAAALERAGIACSERGEKLTLEDFA
                     HIANVWSEMNL"
     gene            14605..14916
                     /locus_tag="VHR42_05020"
     CDS             14605..14916
                     /locus_tag="VHR42_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026570.1"
                     /translation="MESSDHVKKVCDEIVRRFAPEKIILFNLKKDITGGARSFKICVV
                     LETEDKMEVERHIYLDIDSDIPFDVLIYTPEEWKQLGRQKESFAFRIYEKGQYIYGDR
                     E"
     gene            14900..15412
                     /locus_tag="VHR42_05025"
     CDS             14900..15412
                     /locus_tag="VHR42_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX3026571.1"
                     /translation="MVTGSRHREGKSDSKRYNDWLERACEDLKSASILAEDDDTLNTA
                     SFHCQQCIEKALKGYILYKTKNHVDGHNLTWLCRQAIKIDHRFLEWLDESAVLNRFYI
                     ETRYPSDIPLKLNKRTVLRVYEMARVMYLFICDEVGDWDRDCGCEKKKIISGDGNKAS
                     FTVTGEEKNV"
     gene            15658..16515
                     /locus_tag="VHR42_05030"
     CDS             15658..16515
                     /locus_tag="VHR42_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HEX3026572.1"
                     /translation="MRFPKTEKKFSLADTGRFLLKAACVFCCTCFLFTILWGLNYCRR
                     PLERNLGYTVKAPNSKLLENMMEEQTEAVNRLCRKIVFRQSGSVYPGGFQKIKDRVSD
                     GYIQLSEKIRFVRKTPVHPKALILSRLMSYTDISGIFIPFTYEPCVDSEYPEFVLAFN
                     TAHECAHFQGFAKEDEANYIAYLANTENPDPYFQYSAHMMALIYVSNALAQTDRTASN
                     TIFKKLDSRAMGDLRSFNNFGKLHESPFQAISDRINDSYLKLQGQKGIESYDGFVDLL
                     AMGYDEKMR"
     gene            16561..>16880
                     /locus_tag="VHR42_05035"
     CDS             16561..>16880
                     /locus_tag="VHR42_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026573.1"
                     /translation="MKTSNFRYRAAKWLIGLETLMIVLLGIGNIIPVLPFRIGMLSRF
                     SRGYSFFSPEMISFHRSLSTIIGVILIFISYRLYNRVKMAWVIAMCLLPVSLLLRLRP
                     GVGH"
BASE COUNT         4391 a         3690 c         4179 g         4620 t
ORIGIN      
        1 gcaagcaaaa cgtttctatc aagtttaacg aatactgccg taatttcagc cgggaaaatg
       61 attaccgttc cggctggagc atattatgtg agatttgcgg ccgcatcatc tgcgggagct
      121 tttcaactcg aattaggttc aacagctaca acctatgaag cctataaatc ttccatactc
      181 tctattaccg gaacagtcac ggcatcagac cgctttacat gggatggaat taccgggaag
      241 atcgacggtt cagccgtggt aacctccgga aaccttcaag cctatcaaaa cggatgggtt
      301 tttgttcaga attcagatgg gacaccggtc acggttgtac cggcaacgga ggttacctat
      361 aacctgttag gcgatatgag aatatcagct tttgcaaacg gcagcggatc ggcaaacaca
      421 aataaagtcg accatgctgt ttttgccgaa gtcggaacga cccggacgca aggggacaac
      481 agttccaagc tttcaacgac cgcttatgtt gattccggat tgtccgggaa aatgccgatg
      541 agcaacaata cctccggaat cgtcggctat ttcaccaaaa tatccggaac tgtcggggcc
      601 gcatacagtt tgccgacagg cggaacatgg gaatattttg gatttttagt gaatagcagc
      661 acaggcgcga tttttgggcc cacaggcttt ttcgccggcg tttcggcggg aggaacacag
      721 gttgctgctg caacgtccgg ttattatccg gccggttata ttcgtcgaat cgcataacgg
      781 gtaatggagg ccaataacgt ttaactcctg tctttgagtt gctgtcggat atattcaagc
      841 gagagcagtt tttcagtaat ttccaccgtg gatagcctga ttgcattgtt ggaagtatac
      901 tcgctggaaa gacctatttt catattccca gatgtaaaat atttttcgta attaagatct
      961 ctcttgtcaa tcggaacgcg gtaaaattct ccataatcga tggcacggac catttcttcc
     1021 cgtgttaaaa gcgtttcata ctgcttttca ccatgtctgg ttccgatgat tttaatgggg
     1081 ttgtctgcat caaaaagctg tttgacggca agggccaaat ctccgattga ggcggcccgg
     1141 gatttttgga cgataagatc tcccgattct gcgtgttcaa aggcaaagac aacaagctcc
     1201 accgcctcat ccaggctcat gaggaatctt gtcatggcag ggtcggtaat cgtcagcggc
     1261 tgaccggatt tgatctggct gacaaacagt ggaatcacag acccgcggga agccatcacg
     1321 ttgccgtacc gtgttccaca gatcagggtt ttgtcagggt cgatggtttt ggatttggcc
     1381 atgaaaacct tttccatcat agcctttgaa attcccatgg cgttgatggg ataagccgcc
     1441 ttgtccgtag aaaggcaaac aattttttga acccccgact cgattgccga agtcagcacg
     1501 ttgtcggttc ccaacacatt ggttttgacg gcttcaagcg ggaaaaattc acaggaggga
     1561 acctgtttga gtgcggccgc gtgaaaaata taatccacgc cgtgcatgga attccgaacg
     1621 cttgcgatat cgcgcacatc accgatataa taggaaattt ttttattatg atagagtttt
     1681 cgcatatcgt cctgtttttt ttcatctctt gaaaaaatcc ggatttcccg gatgccggtt
     1741 tctaaaaaac gtttgagcac agcattaccg aaagagcctg tgccacctgt aataagcagt
     1801 gtttttccat caaacataac agcaattgtt ctgccgctgc cgcgggcggg aaaagactca
     1861 gctttcctgc ggcggaattc ggaaagcatg gccgggtcta accccatttt atgtagggcg
     1921 ttttggccat gtgaatttgt ggtggaaagg aaaatccccc ggatacggtg attcatgccg
     1981 tgtccggggg atttcttgat aggaggagag aaatcgtatt gaattatagt tttaccgagt
     2041 agtcaaacgc accgctggtc atacaggttc tcgcagaaaa aacggcggca agagacaggc
     2101 tgtgacagag gagcttttcc tgataatcgt ccatatcgct gctttcaaac gtaattcctg
     2161 cttcccgttc cgtacttttt cgaaaaagca ttccgtccag acagcattgc aggaactttg
     2221 aaataaagga atcccctgct cccaactcat cccgggatgg aaccaaatag gctttttgac
     2281 gaagaaaatt ctttccgtcg taaagaagag atcctctttt tcccatggtt cccaccacaa
     2341 tatcgcaccc gcagttatac gcaagataca gaatatcgcg ggcttccgat tctttcctgg
     2401 atccgagaga gagaaacccg aaatccagat gggggcaaat tgttcggagt acttcttcat
     2461 tccattccac tgaaaaatca taggagattt ttcggccggt ctcccgaacc ttctttagcg
     2521 tgtctgcttt cagataagaa taggcgctgg aatgaaccag atcaaaagat cggatatatt
     2581 ccatatcgct gctgtcaaga ataataggat tttgcttttc gtcttcgaga tagttttcca
     2641 taaagattcg atcgccgtta acgatggaaa cctttgccca cgccgtttgg ccctggtaga
     2701 ccctgtggcg cgaagcatca atgtcaaaat gcttcagagc gtcaatttgc agtctgccgt
     2761 tttgatcatt gccgaccgcg ccgagccagg ccgacctgca accgttcatc gcggtgtaca
     2821 cggctacgtt ataggtgttg ccgcccggaa aaacaactcc aagatcttca taaatgtcga
     2881 cgttgttatc accgagacaa agtattttca ttgttttcac ccattatagc tgagttaata
     2941 ttcaactttg tacatgtatc ttctcatcat cagaggatgt cttttggctg cggcaagctc
     3001 tttcatatac accagcgaga ccgaccacag cagagggccg ttgaaataat caatcaccgc
     3061 gtcgtctatg atattaatac caaattcctt gctgtcgata atgtagattt ttttgccgta
     3121 tgtttttaag aattttatag cacggtcatc caaaacgcgg gtttttcctt cattttcaaa
     3181 tacgataaac ggtgtgcgca tatcggtgat ttcaaacggg ccgtgaaaaa attctccgga
     3241 atgaatacaa ttggaatgaa tccattccat ttccataagc aaacagatgc aattcatata
     3301 ggcggcataa tggctggggc cgctgcccat ggtgtagata acgggctcgg ctttacattc
     3361 ctcggcaaat tcccgggctc tttcgctgac catttccacc gctttttcgg ttatcatgcc
     3421 cagcttctcg aaagattcca gcgccaagtc atagttttta taaccttcaa tctggttgag
     3481 caattcaatt ccgagacgaa gtgtcagcac catacagccg ttcagaacat ccgtgtaaag
     3541 atcttcatca taaagcagat gggcgtcgct gaatttcgcg atcggtacgt cattgcgaac
     3601 cgaaatggtg atggaagcgg cgcctttctt tttcgcaagt tcggcggcgg cgacggtctc
     3661 tttggtgttt cccatctggg acatggtcac aacgatggta ttttttccga cgttccgggg
     3721 cacatcgtgg acaaattcat tggccgtgat attatcgaca atcagattgg acgattcatg
     3781 ctgcaggaaa taggcgccgg gattcagacc ggccttggaa cctccgcagg cgacaaaata
     3841 gatgctttta attccgccgt ccgttttttt cctgcttagg aggttggcga cagtcttttt
     3901 gatttcctca tgggatgtta cagtgcagtt agccattaag atacttcctt tcaattcatt
     3961 caaactgata gcaaaaactt gtatcaactt gtattacgat acaacaacat aatacaagtt
     4021 tgcttctcaa ttgtcaagaa gaaatcttaa gacaaatcgg ttcttgtagt cgtgtaatcc
     4081 agtatgttat aatcgaaata agaaaaaacc gacgcaaggt gtagaaatgg gtaacagaat
     4141 gaataattcc gaaaggcctc tttcagggct caaagcggtt cctctctata aacagctgaa
     4201 agcgatgctg cttgagcagc tggaaaatca ggtttggaag aacaacgaaa aaattccatc
     4261 cgaggccgag atcagcggga tttacggcgt aagcagagta acggtgagga gtgcactcaa
     4321 cgagcttgtt gaggacggat atctgataaa aatccagggt aagggtacct atgcctgtca
     4381 tacgattaaa aaaataaccc tctccgcgga ttccggcagc ttccatcagt tttgcaccaa
     4441 ggaaggactg gttccgggga gaagggtgtt ggtttgtgaa agtgtggaag ccaaccagga
     4501 ggatgttgag gagctgcggc tgagcccggg tgatcgggtt gttcatcttt acagtgttat
     4561 gacggtggaa ggcgttccca taacgctcga ggataaccga ctcagcagtc ggtttgattt
     4621 tctaatggaa ctggatctga ccgatcagtc catctggcag ttgcttatga cccacggaga
     4681 cgttcggaaa atcagggtgg cttcccacgt tgtcgacagc agcctgagca attcaaagga
     4741 gtcgcagctg cttcaggtgc ccaagggaac gcccatgctg atcatcaacg agttaaattg
     4801 tgatgaaaat ggagatccgg ttcatcggac aacactcaag agcccgagca accgggtgaa
     4861 attttattat gactggggta atatggggta aaagggataa aaaacgcccc tcccgacagc
     4921 agcccgggag acacacagat cacaggatgt tctgccagtg tttttctttc agaaaatgct
     4981 gcagagccgg gaggtctttc ttttcaaaaa gcctgatgat ctgccggtgc tcatcatttg
     5041 cggcattcag atggtcaaaa agatccggct tcatctccgt aaggtaagcc tgtgggacga
     5101 atgttttacg caaatcgacg agaatgtcga tgagaaagcg gtttttgcat ttatctgtgt
     5161 agacgttgtg aaacaaggtt tgaaattcgg cgtagtccgg gaaattggca tactttatgg
     5221 caatatccat tttgtcggtg agttcataca tacggatgat gtcggcttcc gtgatctggc
     5281 tcatcgcaag cgtcgcggcc agcgagtcaa gggagccgat gatgatgtaa atgtcgtccc
     5341 tgtctttttc actgacttct ttgacggtga aacccttgtt tggggtatac atgaggagcc
     5401 cgtccgcttc gagtttgatc agagcctccc tggttggggt ccggctcagg gaaagttcgt
     5461 tgcagatggt ggattcattg atcttgtcgt tgggcttgag ttcgccgccc tgaattttct
     5521 gaaccagata gttatataca atatttctta gcgattccga tttaaccaat ggagatctct
     5581 ccttgcgctc atagtgttta ctaacaatat aacatatttt atgagggata aatacccttt
     5641 tagacgaaca gttgtttaaa atttaagcag aaagacagga aaattccggg ggccatggct
     5701 tgtcttgaca atgttatcgg attatcctat aattttagaa aaaaggagcg gaaaaattat
     5761 gacaatagat gagatcagag cgttaaacgg taattataag attttcagcg tgacagaccc
     5821 ggaattcaag gcatacggca aagtggtcga gggcctgaat ttttcttcga tgatcgattt
     5881 cgcagaaaaa aaccttgaga ttcccaagga cggaaataaa tatgtggctt ctgtcaaaga
     5941 gctggaagcg tttccggtga tgaaagaagt cgaatatgat atttacggcg agatgccgat
     6001 tgaagccggc ttctgcaccg gccagaatac tgccttgact ggttatgagc ggcatcaggg
     6061 cagtgaagtt aacgtcgcgg tcacggactg catcctgatt cttggcaaac agcaggatat
     6121 ggatggccag actttcgacg gcgcgaaatc tgaaatattc tatgttccta aaaatacggc
     6181 ggtggagctt tacgctacga cgcttcacta ttcgccctgc aaagtttgca acgacggttt
     6241 tatggtgatc gttattcttc tcgagggaac caactccgcg tttgccgccg accacaagcc
     6301 ggctggaacc aataagctcc tgacgaaaaa gaataagttc ctgattgtcc atccgtcgca
     6361 gacggccaag atacaggccg gaacgcttcc cggtttaaag ggtgacatga ttgacattaa
     6421 ggtgaaatag cctttgtccg atcagcgggt taaatttgtt tcaaacgggc ggagcttaca
     6481 ctctgtccgt ttttcttttt gttttgctca gaattgtgtc cgcatgctct ttttttccca
     6541 gcccggcggt gatacaataa aagcaaatag ctcagattgc agtgggcatt tacatcgata
     6601 gatatgttaa tgcgatgatg gaggggtagt atgaaccaag aggaaaaact tgaaaagctt
     6661 aaaaatgacc tgaaatcaat gaaaaggctg attgtcgcct actccggcgg ggtagacagc
     6721 acgtttctgc tccgtgtggc gcaggatgtg cttcatgacg gtgtaattgc ggttaccgcc
     6781 cggtcttcga cctacccgga gcgggagttt aaccagtcta aggaactggc tggcaaaata
     6841 ggcgcccggc atttcattat cgagtcggaa gagctggata ttgacggtta ctcaagtaat
     6901 cccgtcaacc gctgttatta ttgtaaaaaa gaactgttta ccaagattaa cgagctggct
     6961 tccagaatgg gagtccagta tgtcgccgat ggctcaaatg tggatgatct ttgcgattac
     7021 agacccggac gccaggcgtc gggcgaactt aacgtggtca gcccgctgaa agacgcggag
     7081 atgaataagc aggatattcg tgagctttcc agaaagatgg ggctggaaac ctgggataaa
     7141 ccggcgtttg cctgtctggc ctcacgtttc ccttatgggc agcagattac ccatgaaaag
     7201 ctcaaaatgg tggatgaggc agagcagtat ctgatcgacc gggggttccg tcaggtcagg
     7261 gtgcgccacc atggtgaaat agcgagaatt gaggtggcag tggaggaacg gcccaaattt
     7321 tttgatgaaa agctgatgga tgcggttcat gaaaaattcc acaaaatcgg gtttcaatac
     7381 atagcgctgg atctccgtgg atatcggatg ggcagcttga acgaaggaat ggtgaatcag
     7441 tctgcctccc gaaaagccgg ggcggaacag taaatagcgg aaggggattt tatatgcaat
     7501 acagaaaatt cggaaaactg gatgttaaag tatcttcact ggggttcgga accatgcgtc
     7561 tgccgctcaa tagcaacgtg gattcggaca taaatgagcc cgaagccatt cgtatgttgc
     7621 gtcatgcaat cgacaacgga gtcaactata ttgatacggc ctacccctac catcagggga
     7681 aaagcgaaaa tctggttgca aaggctctga aagacggata ccgggaaaaa gtgcttctcg
     7741 ctaccaaact accggtctgg ctcgttaagg aatattcgga ttttgacaaa tatctgaatg
     7801 agcagctgaa aaaacttgag accggacaga ttgattttta cctgctgcac agcctttcga
     7861 aaaaagaatg gaaaaagatg attgacctcg atgtgttcaa agccctcgaa aattataaaa
     7921 aatcgggaaa agtcaaatac atcgggtttt cgtttcatga tgattttgaa acattccgtg
     7981 aaattattga cgcctatgac tgggactttt gccagattca gtataattac atggatgagc
     8041 acgagcaggc gggcaccgaa ggccttcgtt atgcggcttc caaaaacatc ggcgtcgtgg
     8101 taatggaacc gatccggggc ggacatcttt cggccaatcc gccggatcaa gtcaaggaaa
     8161 tctggaattc agcgcctgtg aaaaggtcgc cggccgaatg ggcgctccga tgggtatgga
     8221 atcaccccga ggtgtcggtc gtgcttagcg gaatgagcaa tatcgagcag gtcgaagaaa
     8281 atattcgtgt cgcgtcggct gccgagccgg agtctatgac atccgaagaa cttacgattg
     8341 tcgggaaagt cagagacact taccggggcc ttacaaagat cagctgcacc ggatgccgtt
     8401 attgtatgcc ctgcccgttc ggcgtcagta ttccgggcaa cttcaatatt tacaatgatg
     8461 ctttcgtcta taatatttta gagagcaaac ggaaggaata tcagggcaga gagagcagca
     8521 agctggctgc gaactgccat gactgcggca aatgtgaaga agcctgcccg cagcatctcc
     8581 ccattcgaaa gctgttaaaa gacgtcgacg ccgccctggc tagataaaaa aacagggctc
     8641 agcaggcttt ttcttcaaaa caggagcccc ataccgatgg tatggggctt taggtcttat
     8701 ggtcagacac tgcgctccgc gatttctatg gcttttttaa cgatattacc gcagtcacgg
     8761 cttgaaacag agccccatcc ttcgcgtttc actttatcgt cgactccgag ttcttttgca
     8821 atctcatatt tgagtttttc actcatgatc gaatgcttgc tcatgataag acccccctgc
     8881 ttttttaaat cctgcgcttt tacgcgcagt tctatttttg tccaaattga tcaggatatc
     8941 aaaggtattt ttcaccttga catttaatcc ctgcgtcttt tttgttcgac aagaaaaata
     9001 aaatcataaa tgggcttgag ttgctgaaaa cccgttgaaa gctcatccgc aagacgtggt
     9061 gaaaaaagag tgctcagatc gttggaatgg tgatcaaagc ttatattttt gcggtcaagc
     9121 cagtccatga cctcttccgg ctggccggga aatttgcttc gtttatattt ttcgccgttt
     9181 aactgaaatt ctgtctggtt ttgatacgcg gccaacgcct tttgaaatgc ccgatcgttt
     9241 ttcataatac agtctctgaa aattgccata gattccgtcg tggcttcata ataaccgaag
     9301 ccataagaga aacctccctg ccccaataaa aagtagtagc ccggaaggcc ggcccagaat
     9361 cttttgttcc gcataaaggt gatccacatt tcatcgcgga acagagattt gtcccgtgag
     9421 aaccgcacat cacgatagat acgggctatt gttttgttga cggccggctc cacagtgaac
     9481 aggggatcaa tcgaaagcat gacgggggtc aggtttacaa ccagcgcgcg gagcggctca
     9541 agaacataag tttcatattc tccccgatgc tcttcaaacc aggttttgct gtttttcaag
     9601 cggttttcaa acaaaaaatc caatgttttc tggctgaatc cgttaaacgg aaccgtacct
     9661 tttttattca tagatgtatg gtcgcccctt tcggtaactg ttggtgctgc ggcggtttca
     9721 taccggggtt caaaatcaat ccggttactc gacaaaacct cgatttcggc ctataatggg
     9781 ataaataccc ggtaacagat aaggagactc cgcatggttt ttttacacgc ggtcggcagt
     9841 atccttacgg tcgtgctgat gtttatgaca ggctatttta tgaccgcggc gggatggctg
     9901 gatgaccgga ccagcgacac gttttcaagg attattctga atgtgtcact gccctgctat
     9961 atgctgtata acattgtgag caattttgac cgggcaagac tcgaacagct cagcggtggt
    10021 ctgattgtcc cggttttatc aatcggcatt acctatctga tcggggtggc cgcaagcaat
    10081 ctgcttcatg tgggggaagg acggaaaggt atattccgtt cggtgttttt tacttccaac
    10141 accatattta tcggccttac cgttaatttg tctctttttg gtgaaaaaag cacaccgtat
    10201 gtgctgctgt attatattgt gaacactacg tttttctgga caatcgggaa ttatgaaatc
    10261 agcagggacg gccccggcgc ccacagccaa gcattttttt caagatcgac gttgaaaaaa
    10321 attgtttccc ccgcgcttgg gggatttttg gtggcgctta ttttggtgct gatcggcatt
    10381 aaactgcctt catttatttt ggacgcctgc cgggatatcg gcggaatgac aacgccgctg
    10441 gcaatgctgt ttatcggtat tgtgttgcgt tcggtcaagc tgagagagct gaagcttgat
    10501 cgggagattc tggttttggt tgccgcgcgg tttttgattt gcccgctgac ggtgattgct
    10561 cttgaacatt tcattaaact accaccgctg atggcaaagg ttttcgtcat ccagtccgct
    10621 atgcccgcta tgaccagcac tggggtagtg gccaaaggtt atggggcgga ttatgagttt
    10681 ggaacggtgg ttacggtcat cacaacgatt atgggcatga ttgcgatacc gatttatatg
    10741 tgtgtgatat aaaatgagat agcggctctt ttgcacaatt cttaaaagct gtgaggaaac
    10801 gaggaaaaag tatggcaggt aaattgctcc acagtcgtcg tgcttggagc ccaggcaata
    10861 gggaacgaac ccagcatcat actgttgggc aggataaacg ctgccgcttg ttttttaaaa
    10921 cgccatccaa attctgtctg tatagcagca ggtgggcaag ggtctgatga aacggaaagc
    10981 gaagcaaagt gtatcaaaga ttatctgact tcgcgatatg gtattgaagc caaaagaatt
    11041 tataccgatg atcaatccaa aagcaccagt cagaattttg acaatgcaat aaaaataata
    11101 aaaagatata atttgtctga caatgtggct gtcgcaaccg atggttttca tatgtttcgg
    11161 gcaaagttac tggcacagcg caagggatta aatgcttatg cgtgcccggc tgtaacggat
    11221 aaaagacttg tattaacttt ttacataaga gaattatttg cattaccgaa aacggtactt
    11281 tttaatcaat agaagttgtt ttactccaat aatacagccg cctgcttcca ggcggctgtt
    11341 atgtacttta ggctatgtgc tgtagtaatt gccgtagcaa aacatcttcc attcgctgat
    11401 tcttcggttg tatagtccgc tgacaacttc cccattacag tggcagcacc gggcgaagtc
    11461 ttcacgaagc gtatcggcgg atgcgccgga tttgatgtca ctggtcagct tcggaactga
    11521 atcccagatg ttggctccca gattatagct gaaactgaca agagaatcaa actggttttg
    11581 gcttaaggat gtaccggaaa actcctgatt aacagagttt gaatatttct gaatatcgga
    11641 tttcaaaagt tttaacgcat ccgattttga tagataggtg taattttcac ccggctgaat
    11701 gacatgaccg tacccgattg tttgattctg gctgtcaaca ccacgatacg ccgtagccga
    11761 atagccttct ttggaggcaa taaaattgac cagggaatcc gaaacctgca tgttgccgga
    11821 agaccccgag acccctgttg acgaaacaac cggtgtcgag taaaaaccca acgccaaaat
    11881 actgcttaaa tattcatcaa aagacggccc tttcggtaag tcctttgtat tttcaatggc
    11941 ttcgctgagc atttgatcaa atggaatagc tgagatttct tttccgctgc cgttgctgcc
    12001 ggaggccgca ccggttgaga caggatagag atccgccatc agcaggcttg ctgggtcaat
    12061 catcccatca cctggctttc ttgtttctgt atttttagaa cttgtgatat ttattttatt
    12121 ttaatcccga ttgagcggct tgtcaataaa tttgtcaaaa tttgtagttt tgaggggctt
    12181 ttgcggagca gaacggaatt ccgtctatgt agagcaaaat gccggtctga ccgcaaaagt
    12241 gaaaagagaa cccgcttctt tccatattta tgtatcgggt gtgacttttg ttcgttttaa
    12301 aaagaaaaac caaggagctt ctcacgtcgg ggaagctcct tggtttcaga cgtcgttatg
    12361 ctccgcagac tgttttttcg cggttcggct gcggttttga ttttcgcgcg tcagcggtgt
    12421 ctggctattg ctttttcgtt ttcgcgtttc tttcctgtcg ggctggctgt cttttggcat
    12481 attattttac cctccctccg ggcttacatg atatgatctt cgtgttctgt caggctggct
    12541 ttgaatccaa gccggcttat tttgtccatt atttcaggac cgtttgttcc tgtgctgtca
    12601 taatccaccg caattttttt gtcatgggta ttcacgctga ccgaaattac gccgctgatc
    12661 tggtcaatcc cttttttcag cgcttttgcg tcatggcggg ttacgatgtc atccagtgaa
    12721 taataaacgc tttgttttga caagctaacg cctccttttt gtcaggaaac catcattatt
    12781 attacacgct gatgaccggg tatgtataaa aaaagccgga caatccggct taaagaaaag
    12841 aggacggtga tttaatagcc catgctgttt tccaatgact cgtcgtgttc aacccattcc
    12901 cttttcacat caatgatggt atagtcatcg tgtgtgcagc gaataatgac tttagcgatt
    12961 cccgcgttga tgatcatgcg cttgcacatg gcgcaggagt tggcgccttt gacaagctca
    13021 ccggtggcgg cgtcctgacc gacaagatag agagtggagc cgatcatgtc gttacgggcc
    13081 gcagcgataa ttgcgtttgc ttccgcgtga acagaacggc aaagctcata ccgctcgccg
    13141 cggggaattt ttagtttttc cctcatgcag gtccccaggt cggagcagtt tttcctgcct
    13201 ctgggcgcgc ccgtgaagcc ggttgcgata atttgatcgt tgcttacgat aattgcgcca
    13261 aagtttctgc gcaggcaggt tcctcgctct aaaaccgttt gggcgatgtc aagataataa
    13321 ttttgtttat ctcttcgttg catcgggggt gctccttcca ttataatgaa taaaggcgat
    13381 aaaagcttaa ttctgtctaa aaacattata tccaatcatc cggccaaaat caatgcggga
    13441 ataacttgtc gtaacagttt ttctctttgc ggctgcttga ggagaaaatc aggcactgca
    13501 tggttgaaga tgaaacagcc ttgagagttg ttttaagctt ttaggaatgt cgtaaaatcg
    13561 aatcgctgtg tgatgaacca cagcaggaaa cccgggggat aaatgaatga atcatcttac
    13621 cgatgccgca tatgtcagga agctacttga aagttacgga ttcaattttt ccaaggcact
    13681 gggccagaat tttctcatca atccgtctgt ttgcccgaaa atggctgaac tgagcggagc
    13741 cggtaaaggc gtcggggcga tcgagatagg gcccggagtg ggggttctta ccgcggagct
    13801 tgccgaaagg gcggaaaaag ttgttgcgat cgagctggat tcccgcctga ttcccgtact
    13861 ccggcggaca cttaaagatt atgataatat caggctgatc aatggcgacg cgctggagac
    13921 cgacctgaag gaaattgtaa caaacgattt tgccggtttg gaagccgtcg tatgtgcgaa
    13981 tcttccctat tacatcacgt caccggtcat tatgcatctt ttggaatcga aacttccggt
    14041 caatagcata acggtgatgg ttcaaaagga ggcgggcaga agaatctgcg ctgtccccgg
    14101 cagccgtgag gcaggcgccg tcagccttgc cgtccgctac tattcggaac cggccatcct
    14161 cttcgacgta tcttccggca gttttatgcc ccggccgaaa gtcgattcct gtgtgattcg
    14221 gctggatgtc agaagcagtc ctccggtgga tgtaaaaaat gagaaactgc tgttccgaat
    14281 catcagggcg gcgtttaatc agcgcaggaa gacccttctc aacggtctgt cggcagggct
    14341 tggccaatcg aaggaacgga cggccgcggc gctggagcgg gcaggaatcg catgctcgga
    14401 acggggagaa aaattaactc tggaagactt tgcgcatatt gcaaacgtat ggagtgaaat
    14461 gaatctgtaa aatctttcaa atattaagcg ctgtgtattg tttccattgt tttaagagaa
    14521 tataatgaaa aaggaatcag cctgttttgt ctgatctgag gccgtattcc tgatttgggt
    14581 tgttttcatg ggaggtagat attaatggag tcaagtgatc atgtgaaaaa agtatgcgat
    14641 gaaatagtca ggagatttgc cccagagaaa ataatcttgt ttaatttaaa aaaagacatc
    14701 acgggaggag cccgcagttt caaaatctgt gtcgttctgg agaccgagga taaaatggaa
    14761 gttgaacgcc atatctatct ggatattgac agtgatattc cgtttgacgt gctgatctat
    14821 acccctgagg aatggaaaca gctgggccgt cagaaagaaa gctttgcttt caggatctat
    14881 gaaaaggggc agtatattta tggtgacagg gagtaggcac agggaaggaa aaagtgacag
    14941 taaacggtat aatgactggc ttgagcgtgc ctgcgaggat ttaaaatcag cttcgatcct
    15001 ggcggaagac gacgatacgc tgaacacagc gtcgttccat tgtcagcagt gcattgaaaa
    15061 ggctctgaaa ggatacatcc tttataaaac aaaaaaccat gtcgacggtc ataatttaac
    15121 ctggctctgc cgtcaggcca tcaaaatcga ccaccgtttt ctggagtggc tggacgaaag
    15181 cgcggtgctg aaccggtttt atattgaaac aagatatccg tccgatattc cgcttaaact
    15241 gaataagcgt acggttctcc gggtttatga aatggcaagg gttatgtatc tgtttatttg
    15301 tgatgaagtt ggtgactggg atcgggattg tggctgcgaa aaaaagaaga ttatatccgg
    15361 tgacggcaat aaggcctcgt tcacggtcac aggtgaagaa aagaatgtct gataggcgcc
    15421 gaaacacccg gcagggccgg cccgtttatg ccgctctgct tcttcttttg cctgtgtcta
    15481 tggttttaaa tttcttttgc cataaaaacc cggatctgac agaagaatta tatagcagaa
    15541 agatctaccc cgcgattgcg catttgtcgt gtttgttcgg tctgttctcg ttttcagtgg
    15601 ctgaagcggt tctgtttctg ctgattgccg ctgtactttg gctcgtgatt cgccgttttg
    15661 cgattcccaa aaaccgaaaa aaaattcagt cttgcggata caggccggtt tctgttgaaa
    15721 gcggcctgcg tattttgctg cacttgtttt ctgtttacca tactgtgggg actcaattac
    15781 tgccgcaggc cgctggaacg caatttaggt tatacggtta aagctccgaa cagcaaattg
    15841 ctcgaaaata tgatggaaga gcagacggaa gctgttaacc gactttgtcg aaaaattgtt
    15901 tttcgacaaa gcggctcggt ttatccgggt ggttttcaaa aaataaagga ccgtgtcagt
    15961 gacggttaca tacaattgtc ggaaaaaatc cggtttgtcc gcaaaactcc ggttcacccg
    16021 aaggctctga tcctgtcgcg gctgatgtcc tataccgaca tcagcggaat ttttattccg
    16081 tttacttatg agccttgtgt tgattcagaa tatccggaat ttgtccttgc ctttaatact
    16141 gctcacgaat gcgcccactt tcagggcttc gccaaagaag atgaggcaaa ctatattgct
    16201 tatcttgcaa ataccgaaaa tcccgatcct tattttcagt attccgcaca tatgatggct
    16261 ctgatctatg tttcaaacgc cctggcacaa accgaccgga cggcatcgaa taccattttt
    16321 aaaaaactgg attcccgggc gatgggggat ctgagatcct tcaataactt tgggaagctt
    16381 cacgaaagcc cctttcaggc gatttccgac cggatcaatg attcctacct gaaattgcag
    16441 ggccagaagg ggatcgaaag ttatgacgga tttgtcgatc ttttggccat ggggtatgat
    16501 gagaagatgc gttaagaagc gcgatttccg gcgggattgc cggcaaaatg ggggaaatat
    16561 atgaagacca gcaatttcag atatcgggcg gcaaaatggc tgattggtct ggaaacattg
    16621 atgatagtcc tgctcggaat cggcaacatt attccggtac ttccttttcg gatcggcatg
    16681 ctttccaggt tcagccgggg atattctttt ttttcgcctg agatgatttc ctttcatcgc
    16741 tcactttcta caataattgg cgtaattctg atttttatca gttatcgtct ttacaaccgg
    16801 gtaaaaatgg cgtgggtgat tgccatgtgt ttactgccgg tttctttgct ccttcggctt
    16861 aggccggggg ttgggcattt
//
LOCUS       DATALY010000171         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_726277,
            whole genome shotgun sequence.
ACCESSION   DATALY010000171 DATALY010000000
VERSION     DATALY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726277"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VHR42_05040"
     CDS             <1..737
                     /locus_tag="VHR42_05040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX3026574.1"
                     /translation="GIKPILVGAAAFFVFALFLEQLLHSLVLHQVPAITSDPLLYAAY
                     GGIAAGVFEETGRLVGFRFLLNKNSQWEQGVAYGLGHGGLELVCIGGLLAITEGQSFY
                     YSIQINDGSFSQVIANAKDVPLQQSNLRMIQQQLTSAPSWEFLMGGLERVFALVIQIA
                     LSLVVLYAVSKGKYIFYALAVLLHATVDFTAAYLNRIQVPTPVIEGIVAVFAGIALFA
                     IFRMKPLFTGGGELQHKLPKEVKRAD"
     gene            761..1192
                     /locus_tag="VHR42_05045"
     CDS             761..1192
                     /locus_tag="VHR42_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HEX3026575.1"
                     /translation="MKKKILAVCLAILSVSVLAFVGCVSSQKYEKLSSKFSEAKVKSS
                     AEEVVTNINAAKFEAVCGNMSETLKGSLPAEALQKAVSPFLSKAGAFDSFSSETVSGL
                     LDTKSGQEYATAVVMAKYKNQTVQYTITFDTDMKMAGIYFK"
     gene            complement(1271..2086)
                     /locus_tag="VHR42_05050"
     CDS             complement(1271..2086)
                     /locus_tag="VHR42_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX3026576.1"
                     /translation="MFQYAFMQDALFVSVCIAILCPCIGVFLVLRRYSMIGDTLSHAS
                     LAGITIGLACGGSPVIGAFLFTSVCGALIEFLRTYFKKYTDLILTIVLSLSVGVAITI
                     ISSGKLHANADAFLFGSILTVDQFDKLTVLVLTILSILAIIFLYHEMLYIAYDEEAAQ
                     VAGVRVKVINYVFSILVASAISVSIRVVGVLVLSSMIALPVAAALQLKKGFRTTLLFS
                     ILFSMVDILLGLFFSYQLNVAPGGFTALVSVAVLVIVMIFRKIAMQVRQIRTA"
     gene            complement(2086..2766)
                     /locus_tag="VHR42_05055"
     CDS             complement(2086..2766)
                     /locus_tag="VHR42_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX3026577.1"
                     /translation="MSKLIEAKNLTFSYTGSPPFVLDGINLKIEDGNYVSVLGENGCG
                     KSTLMRLILRFLKPTSGTSLCKANRIGYVPQKNDFSNPDFPITVYEMLDSYRQLLKIH
                     DKDAVKKSLALVGMEDYKKALMGSLSGGQGQKIRIARATLGRPELLVLDEPSTGVDFA
                     SRKDIYGFLTRANRKYGITVVSVEHNLEAAIESSTLIYHLDHGKGHLCTPQKYAEEFL
                     EPEKGGRH"
BASE COUNT          761 a          646 c          743 g          721 t
ORIGIN      
        1 ccggaattaa gccgattttg gtaggtgcgg cggccttttt cgtttttgct ctgtttctgg
       61 aacagttgct gcattcgctg gtcctccatc aggttccggc aattacaagc gatccccttc
      121 tttatgccgc atacggcgga attgcggcag gtgtttttga agagaccgga agactggtcg
      181 gctttcgttt ccttctgaac aaaaacagtc aatgggaaca gggcgttgcc tacggccttg
      241 ggcatggtgg tctggaactg gtgtgtatcg gcggcttact ggcgatcact gagggccaga
      301 gcttctatta ttcaattcag attaacgacg gctcgtttag ccaggtgatt gcaaatgcaa
      361 aggatgtccc gctgcagcag tccaacctga ggatgattca gcagcagctg acaagcgcac
      421 caagctggga atttttaatg ggcggccttg aacgcgtgtt tgctcttgtc attcagattg
      481 ctctgtcgct ggtcgtcctt tatgcggttt caaaaggaaa atacatattc tatgctcttg
      541 cagttcttct gcatgcaacg gtggatttta ctgcggcgta tctcaatcgc atccaggttc
      601 cgacacccgt tatcgaaggc attgttgccg tgtttgcggg aatcgcactt tttgccatat
      661 tccgcatgaa gcccctgttc acaggtggtg gagagctgca gcataaactt cccaaagaag
      721 tcaaacgagc ggactgagaa tagtaaggag agttgctgtt atgaagaaaa agattttggc
      781 tgtttgtctg gcaattctga gtgtttcggt tcttgctttt gtcgggtgcg tttctagtca
      841 gaagtatgaa aaactttcct caaaattcag cgaagcgaag gttaaatctt ctgcggaaga
      901 agtcgttaca aacataaatg ctgcaaagtt tgaagcggtg tgcggcaaca tgtcagaaac
      961 cctaaagggt tcgcttcccg cagaagcatt gcaaaaggcg gtgtcgccgt tcctgagcaa
     1021 ggccggggca tttgattcct tttcttcgga aacggtttcg ggtcttctgg acactaaaag
     1081 cggtcaggag tatgccacag cggttgtcat ggcaaaatac aaaaaccaga cggtgcagta
     1141 caccattacg ttcgacactg atatgaaaat ggcagggatt tatttcaaat aatttagagt
     1201 taaaatagaa aaggaacggg cttcggaaaa agcccgttcc ttttttgtgt tccgatgatt
     1261 tctttcggtt ttaggcagtt ctgatctgcc gtacctgcat agcaatttta cgaaagatca
     1321 tgacaattac aaggaccgcg accgaaacaa gcgccgtgaa accgccgggt gcaacgttga
     1381 gctggtagga gaagaaaagc ccgagaagaa tgtctaccat gctgaaaagg atggaaaaca
     1441 gaagagttgt gcggaatccc ttcttcagct gaagtgctgc ggctacaggc agagcaatca
     1501 tggagctgag aacgagcacg cccacaactc ggattgaaac cgaaatggca gatgccacaa
     1561 gaatggagaa aacgtagttg attaccttaa cgcgaacgcc ggcaacctgc gccgcttctt
     1621 cgtcgtaggc aatgtagagc atctcatggt aaagaaaaat aatagcgaga atcgagagaa
     1681 tggtaagaac cagtacggtc agcttatcaa actggtccac cgtcagaatg ctgccgaaca
     1741 ggaacgcgtc cgcattcgca tgaagcttgc ccgaactgat gatggtgatt gccacgccca
     1801 cactcagtga aagcacaatc gtcagaatca aatcggtata ttttttgaaa taggtgcgga
     1861 gaaactcaat caaagcaccg cagacggatg taaacagaaa ggctccgata accgggcttc
     1921 cgccgcaggc aaggccgatg gtgatacctg caagcgacgc atgagaaagt gtgtcgccga
     1981 tcatcgaata gcggcgaagg acgagaaaaa ccccgatgca cgggcagaga attgcaatgc
     2041 agacggatac aaacagcgca tcctgcataa aagcgtattg aaacattaat gtctgcctcc
     2101 cttttccggc tccagaaatt cttccgcata tttttgggga gtacagagat gacctttccc
     2161 gtggtcaagg tggtaaatga gtgtggagct ctcaatagct gcctcgaggt tatgttccac
     2221 ggaaacgacc gttattccgt atttgcggtt cgcgcgggtc aaaaaaccgt aaatatcctt
     2281 ccggctggca aaatcgactc cggtagaagg ttcatccaga acgagaagtt ccggtcggcc
     2341 gagtgtggcg cgcgcaatgc gaattttttg tccctgcccg ccggaaagcg atcccatcag
     2401 cgcctttttg taatcttcca ttccgacaag tgcaaggctc ttttttaccg cgtctttgtc
     2461 gtgaatcttc agcagctggc ggtaagagtc cagcatttca taaacggtaa tggggaagtc
     2521 cgggttcgaa aagtcatttt tctggggaac gtagccaata cggtttgcct tacagagact
     2581 tgtgcctgat gtgggtttga gaaagcggag aatcagccgc attaacgtgc tctttccgca
     2641 tccgttttct ccaagcacgg aaacgtaatt gccgtcttca attttcaggt tgatcccgtc
     2701 aagcacgaaa ggcggggaac cggtatagga aaatgtcaga tttttagctt ctatcaattt
     2761 agacaccagc cctttgtttt atgacgcgcc cgaagactat tgacaagcag gccgccaaga
     2821 attattataa atgcaaatga cattcatttg caatagctat tttggaggaa t
//
LOCUS       DATALY010000172         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_37737,
            whole genome shotgun sequence.
ACCESSION   DATALY010000172 DATALY010000000
VERSION     DATALY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37737"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..459
                     /locus_tag="VHR42_05060"
     CDS             166..459
                     /locus_tag="VHR42_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026578.1"
                     /translation="MGQAKDLEITEFHTLIAEIRAQTDLLKETILTEDEKQEWNKILT
                     DFDNSILNQVYGTLYHLSKYQLEGWRELIDGEGDGEKASPGTSACGGPSVSQR"
     gene            386..685
                     /locus_tag="VHR42_05065"
     CDS             386..685
                     /locus_tag="VHR42_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026579.1"
                     /translation="MEKVTEKKHLPELLHAAVRQFRSGEDAAGMEDLRKAVDELEAAV
                     EADQESLQFSMDKLLPALRELYGYMQNRDIAGLTDWLEYTAATMPEECPEGCGEL"
     gene            682..2610
                     /locus_tag="VHR42_05070"
     CDS             682..2610
                     /locus_tag="VHR42_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HEX3026580.1"
                     /translation="MKIAEPNETEAMYEQNLSLLTPWLRDTLAKISPKELKEKIEVTY
                     NPEGYPVCRYHRDGLCFHVTSEHPIEEAETWYRSIRHVGSSETLLYGMGFGYALFEVF
                     AHKPPHTLVVVFEQDIFLFKAMLYYFDLSPIIKTQKIVIFIGDSSYFKDSFEKLFSTM
                     RFFSTTYPTTARTLPAMRNFKKGYQEIHRFIYRELTLLASYLGNDHQDNMIGLRNLTA
                     NTKTVLENPYLSCLKDKYKNVPAFIISNGPSLDLSMPLLKEIQGRGLMIAVESAIVPL
                     TANGIHPDILAVQERTKYTYLYHFEGRHYSSDISLLALTMVDPRVFPSFPGEKIPIYR
                     LGEELNRWFSRHLGGSNDLDAGANVSHLAAEEALYLGADPIIFVGQDFAYGPGGVTHS
                     RDAVASQEKGKRARDLLHSIPTVYVEGNNGEMIPSNQLWENFRLGLEHIIGLYPDHHF
                     YNATSGGAKIRGTEYAKLDQLIQQYCTRPIPYRVYEIIAESRKMISHSEREKLLEKFI
                     GEMETCAVRFRNLAGEMNQKKLECEKMMFLCSKENHGINYKLLNETYQKNIEAFYRYS
                     DDELCRSFFQQLNCAYFHLLDRLGKINTIAKIAQAFGVQSQFFHDLGAVSQSLSISLE
                     EDARDLNALLEEYHKKEA"
     gene            complement(2648..3382)
                     /locus_tag="VHR42_05075"
     CDS             complement(2648..3382)
                     /locus_tag="VHR42_05075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026581.1"
                     /translation="MEKKKQLSLCVITKNDAEFLPECLNDMKDIADEILVADLGSEDR
                     TPELAEQAGVAVYRTKWENDFSKIRNFCMERASGKWVLFLQADEVLSQEQHAELSLLL
                     KNPNAEGYLIYVDYNPKERGISSPAQFLRLIRNRKEYCFHYRSFPYIPDEALYSLQNC
                     SLRITHRREKTIGWQLEEKHTLLKEDLRENPADSFLRYMEGIELLNREKFEESTGSFE
                     MARRAVTGGYLSYHSGIRAKIPRRFL"
BASE COUNT          917 a          768 c          854 g          880 t
ORIGIN      
        1 cacgcggctt tttgagatgc cgccggagcg cgccgccgat attgactccg aactggattt
       61 tgaatttgtg gaattcctta tgaaaaagag ggctgaacgg tgtggttcgg ccattttgtg
      121 atgaacggcc ccgaccggga gtatacgaca gaaggaggag cacggatggg acaggcgaag
      181 gacctcgaga taacggagtt tcataccctg attgcagaaa taagggctca gaccgatcta
      241 ctgaaagaga cgattctgac ggaggatgaa aaacaggaat ggaacaaaat actgaccgat
      301 tttgacaact cgattttgaa tcaggtttac ggcacgttgt atcatttatc caagtatcag
      361 ttagagggat ggagggaatt gattgatgga gaaggtgacg gagaaaaagc atctcccgga
      421 acttctgcat gcggcggtcc gtcagtttcg cagcggtgaa gacgccgcgg gcatggagga
      481 tttacgaaaa gcggtggatg aattggaagc tgcagtcgaa gcggatcaag aatccctgca
      541 gttttcaatg gataagctgc tgcccgctct gcgtgagctg tatggttaca tgcagaaccg
      601 cgatattgcc gggttgaccg actggctcga atacacggct gctaccatgc ctgaagagtg
      661 tccggaagga tgcggggaat tatgaagatt gcggaaccga acgaaacgga agcgatgtat
      721 gaacagaacc tttcgctgct gacgccgtgg ctgagggata cgttagctaa aatttcaccg
      781 aaagaactga aggaaaaaat cgaagtcacc tataatcccg agggttaccc tgtctgccgg
      841 tatcacagag acggactctg tttccatgtt acaagcgaac atcccattga ggaagcagaa
      901 acttggtatc gttctattcg gcatgtcggt tcgtcggaaa ccttactgta cggcatggga
      961 ttcggatacg ctctgtttga agtttttgcc cacaagccgc cccatacgct ggtggttgtg
     1021 ttcgaacagg atatctttct cttcaaggcg atgctgtatt attttgatct gtcaccgatt
     1081 attaaaacgc aaaaaatcgt gatttttatc ggggacagtt cttatttcaa agattcgttt
     1141 gaaaaattat tctccaccat gcgctttttc agtacgacgt atccgaccac agcgaggact
     1201 ttaccggcga tgcgcaattt caaaaaggga tatcaggaaa ttcaccggtt tatctaccgg
     1261 gaactgactt tgctggcctc ctatcttgga aatgaccatc aggataatat gatcggtctt
     1321 cggaatttaa cagccaacac gaaaacggtt ctggaaaatc cgtatttgag ctgtttgaag
     1381 gacaaatata aaaatgtccc ggcatttatt atttccaacg gaccgtcact ggatctcagt
     1441 atgccgctgc tgaaggaaat tcagggccgc ggcttgatga tcgcggtgga atccgccatt
     1501 gtgccgctta cggcaaacgg aatccatccg gatattctgg cggttcagga gcgtacgaag
     1561 tatacctacc tgtatcattt tgaaggccga cattattcgt cggatatttc tctccttgcg
     1621 cttacgatgg tcgacccgag ggttttccct tcctttccgg gagaaaaaat tccgatttat
     1681 cgtctgggag aagaactgaa ccgctggttc agccggcatc tggggggctc aaacgaccta
     1741 gatgcggggg caaatgtgtc ccatcttgcg gcggaggagg cgctgtacct cggtgccgat
     1801 cccattatct tcgtcggtca ggatttcgcc tacgggcccg gtggcgtgac acacagccgt
     1861 gatgcggtcg cctcgcagga aaaagggaaa cgtgcccgcg acctgcttca ttccatccca
     1921 acggtttatg tggaaggaaa caatggggag atgatcccat ccaaccagct ttgggagaat
     1981 ttccggcttg gtctggaaca cattattggg ttatatccgg atcaccattt ttacaacgca
     2041 acgtccggcg gggcaaagat acgaggaaca gaatatgcga agttggacca attaatccaa
     2101 cagtactgca cccggccgat cccgtatcgg gtctatgaaa tcattgcgga aagcaggaaa
     2161 atgatttctc acagcgaacg tgaaaaactg ctggaaaaat tcatcgggga aatggagact
     2221 tgtgccgtcc ggttccgaaa tctagctggg gaaatgaatc aaaaaaaact ggaatgcgaa
     2281 aaaatgatgt tcctgtgttc gaaagaaaac catggaataa attacaagct gctgaatgaa
     2341 acctatcaaa aaaacattga agcattttat cggtattcgg atgatgaact gtgccgcagc
     2401 ttttttcagc agttgaactg cgcttatttt catttgctgg atcgtctggg caaaattaac
     2461 accatagcaa aaatagctca ggcattcggc gttcaaagtc agtttttcca tgatctgggc
     2521 gcagtcagcc aaagtctgtc catttctctg gaagaagatg cccgggacct gaatgctctt
     2581 ttagaagagt atcacaaaaa ggaggcttaa aaagtggaga aaaaaaagca attgagcctc
     2641 tgcgtcatta caaaaaacga cgcggaattt ttgcccgaat gcctgaatga tatgaaagat
     2701 atccgccggt gactgcccgg cgtgccattt cgaacgatcc cgtactttcc tcaaactttt
     2761 cccgattcag aagttcaatt ccttccatat accgaagaaa actatccgcg ggattttctc
     2821 ttaaatcttc tttcagcaga gtatgctttt cctccaattg ccatccaatg gttttttctc
     2881 ggcggtgcgt gatgcgtagg ctgcaattct gcaaagaata caatgcttca tccggaatat
     2941 acggaaagga gcggtaatga aagcaatatt cttttcggtt ccgaatcaac cgaagaaact
     3001 gagcggggga agaaataccg cgttctttcg gattgtaatc cacataaata aggtaaccct
     3061 ccgcattggg gtttttcagc aacaggctca gttcagcgtg ctgttcttgg gagagaacct
     3121 cgtccgcttg caaaaacagt acccattttc cagaggcgcg ctccatgcag aaatttctta
     3181 tcttgctaaa atcattttcc catttcgtcc gatataccgc tacgcctgcc tgttccgcta
     3241 gttcgggagt tcggtcttcg gaaccgaggt ccgcgaccag tatttcgtcg gcgatatctt
     3301 tcatatcatt caggcattcg ggcaaaaatt ccgcgtcgtt ttttgtaatg acgcagaggc
     3361 tcaattgctt ttttttctcc actttttaag cctccttttt gtgatactct tctaaaaga
//
LOCUS       DATALY010000173         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_726868,
            whole genome shotgun sequence.
ACCESSION   DATALY010000173 DATALY010000000
VERSION     DATALY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726868"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VHR42_05080"
     CDS             complement(<1..548)
                     /locus_tag="VHR42_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX3026582.1"
                     /translation="MRYIPTSCLRENMVLAKSLYGSDNVLLLQKGQLLKEPYISCISN
                     YGFQGVYIDDKMSEDIEIKNVIDDELRNSAVKAVRDIYVQSQTVGITRNMANGAKAAV
                     HSIVEEIIDNRDIMVNMVDLKTYDDYTYYHSVNVGVLSIVLGVALGMNRSSLYKLGLG
                     ALLHDLGKVFIDKAIIDKEGPLT"
     gene            802..1455
                     /locus_tag="VHR42_05085"
     CDS             802..1455
                     /locus_tag="VHR42_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3026583.1"
                     /translation="MKELFNKIREEKRNRILMAATNEFAKNGFEHTNVNEIAKNAEVS
                     VGSLYKYFDSKRDIFLTTVRYCASILKTVLDGVMKDNDDWQIKVEKIIHAIQVYSRES
                     KVIIQLYNEMATQSNSGIVIETVEEVEALSAELYSGLIQKEKQKGGIRADCDSRMFAF
                     FLDNLFMMLQFSYACDYYRERFKVYVSSDILQRDEFVSEQMLKFIRGAFTAENPEAK"
     gene            complement(1477..2478)
                     /locus_tag="VHR42_05090"
     CDS             complement(1477..2478)
                     /locus_tag="VHR42_05090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3026584.1"
                     /translation="MVKKLLCFAILTAFLWSAFIPPACAQVSAPKNLLLFGDSISTGY
                     GLSNPGKSFGSLLKKALAAPNYKDLAVNGATSANVLAAAKNHKEEIRTADTILLTAGG
                     NDVLNVFFSSIRSSLGLPENAPARQILENLIGKKNALDQIEAKLKDTQTQAKLKAAEM
                     GFAQNLPAIVSEIHTTNPRAKLYIQTVYNPFSDESDLFPFAGLADEILSHINTEIKND
                     AAGKYTVIDSYSAFRNQALTLTNIAGFDIHPSEAGHAVIFRLAYEAVTGKQYAVTSSG
                     SRAPGSGAPSAAGNSSSLSSGSALGSSSGKLEAGAEIAGDAALLLLAGVGVWKRKKR"
     gene            2764..2931
                     /locus_tag="VHR42_05095"
     CDS             2764..2931
                     /locus_tag="VHR42_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026585.1"
                     /translation="MSESKLLTVFRRLKERKAESGMTADDAEMAFIDRMIRELESDAQ
                     KQRVPAEPWKR"
     gene            complement(2928..>3638)
                     /locus_tag="VHR42_05100"
     CDS             complement(2928..>3638)
                     /locus_tag="VHR42_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026586.1"
                     /translation="QTLTVNQLYSSIKRVNEIAENTTREMDHAVGSIGRIFERIIDGD
                     NQMDQLLKAEEKTRQSSGEIQKVNRMISDIAQRTNLLALNAAVEAARAGGAGKGFAVV
                     AAEVRNLAGQSAEAAKQTALMIDASIRSVEESSSIAADTANSLSESLKEIETVSKTIQ
                     EVRQDLEKQTASMQRMTQDVGAISAVVEKNTAAAKEGAAASQLLLSRAELLKEKIGRI
                     ALRDTFDSDASEVCDEVV"
BASE COUNT          909 a          866 c          925 g          938 t
ORIGIN      
        1 gtaagcggcc cttctttgtc aatgatggcc ttatcaataa acacttttcc gaggtcgtgc
       61 agcagtgccc ccagcccaag cttataaagg ctgctccgat tcattccaag cgcgacgcca
      121 agaacaatcg aaagcacacc gacgttgacg gaatggtaat aggtataatc atcgtatgtt
      181 ttcaggtcaa ccatattgac cataatgtcc cggttgtcga taatttcttc cacaatgctg
      241 tgaacggccg cctttgcgcc gttcgccata ttccgggtga tcccgaccgt ctggctctga
      301 acatagatgt cccgcacggc tttgacagcg gagttcctca attcatcatc gatgacattt
      361 ttaatctcaa tgtcttccga cattttgtca tcaatataca cgccctgaaa accgtaattc
      421 gagatgcaag aaatataggg ctccttcagc agttgccctt tttgcagcag aagaacgttg
      481 tcgcttccat atagactttt ggcgagcacc atattttccc gaagacaaga tgtgggaata
      541 tatctcatag atttgcctcc gcagacgaaa tagtgggaat ttgcttcaaa gcaaaatttg
      601 gtccgttaaa aagatctcaa ttctattatt ctaatccttc tgtacaatat ttcaataggc
      661 aaagaggcag aatgcaccaa aatgttttat aagtcacatt tttcatcgga taatctatcc
      721 gatgagactt gattcggcat tttatgacaa ctcgggttgc tttggaaggc agtgatccgg
      781 tgggtataaa ggagatttta tatgaaagag ctgtttaaca agatccggga agaaaagcga
      841 aacagaattc tgatggcggc gacaaacgaa tttgcgaaga acggttttga gcacacaaat
      901 gtcaatgaaa ttgccaaaaa tgcagaggta agcgtcggca gcctctataa atatttcgat
      961 agtaaacggg atattttcct caccacagtc cgatactgtg cgtccattct gaaaacggta
     1021 cttgacggcg tgatgaagga taacgacgac tggcagatca aggtggaaaa gataatccac
     1081 gccatccagg tgtactcccg cgaaagcaag gttattattc agctctataa tgaaatggca
     1141 acgcagagca attccggcat tgtgatcgaa acggtggagg aagtggaggc tctttccgca
     1201 gagctgtatt ccggactgat tcaaaaggag aagcaaaagg gcggcattcg agccgactgc
     1261 gacagcagga tgttcgcgtt ttttctggat aatcttttca tgatgctcca gttttcctac
     1321 gcctgcgatt attacaggga acggtttaag gtgtatgtca gttctgatat attgcagcgg
     1381 gacgaatttg tttcggaaca gatgctgaaa tttatccgcg gggcattcac cgccgagaat
     1441 ccggaggcaa aatagaaatt ccccgcgtct ttcggcttac ctctttttcc gtttccagac
     1501 cccaaccccg gcaagcagca ggagagcggc gtcgcctgca atttccgccc ccgcttcaag
     1561 cttccccgaa gatgacccca acgcagaacc ggaggatagg gaagaggaat tccccgcggc
     1621 cgacggggcc ccggagcctg gagcccgcga accgctggaa gtgacggcat attgcttccc
     1681 tgtgaccgcc tcataggcta aacggaaaat aacggcgtga cccgcctcac tcggatgtat
     1741 atcgaatccg gcaatgttgg tgagcgtgag tgcctggttt cggaaggcag aataagaatc
     1801 aatgaccgtg tatttccccg ctgcgtcgtt tttgatttcg gtgtttatgt ggctcagaat
     1861 ttcgtccgcg aggccggcga acgggaaaag atcgctttcg tcgctgaatg ggttataaac
     1921 ggtttgaata taaagctttg cccggggatt tgtcgtatgt atttccgaaa cgatcgccgg
     1981 aagattctgt gcgaagccca tttcggcagc ttttagcttt gcctgcgttt gggtatcttt
     2041 cagcttcgct tcgatttggt caagggcatt ttttttgccg atcaggtttt ccagaatttg
     2101 acgggcgggt gcgttttccg gtaagccgag agacgaccgt atggaggaaa aaaagacgtt
     2161 cagaacgtcg ttgcctcctg ctgtaagaag aatggtatcc gctgtgcgga tttcttcttt
     2221 atggtttttt gcggctgcca gaacgttggc agaagttgct ccgttgacag ccaggtcctt
     2281 ataattgggg gcggcaagcg cttttttaag caaacttccg aaactttttc cggggttgga
     2341 aagaccgtac ccggtcgaaa tggaatcccc gaacagcaaa agattttttg gagcgctgac
     2401 ctgcgcacag gctggcggaa tgaaagcgga ccagaggaaa gcggtcagaa tagcaaagca
     2461 aagcagcttt tttaccatgg ttctttctcc ctcaagtgag gatgtttttc ttttatttta
     2521 tcacatttca tcaggaagga caccggtttg ttttcccagc ggatcgatcc cgcgaaaaag
     2581 aaaatcgcgc ggccgcgata accgtgcgat gatgaaagaa gcgggattct tataaaaagg
     2641 gaaggtaagg cgccgtttcg gaaggagcct gtcgaacgat accgcaggtt ttaagcaaat
     2701 ttaatccaat cccaatacaa tgcaaatcaa tccgccttac tatagaaggc ggaggtgata
     2761 cgaatgtcag aatctaaatt gctgacggtt ttccgcaggc tgaaggagag aaaggcagaa
     2821 tcgggtatga cggcggatga tgcagaaatg gcctttattg accggatgat ccgggaactg
     2881 gagtcggatg cacagaaaca gcgggtaccc gccgaaccct ggaaacgtta aaccacttca
     2941 tcgcacacct cggatgcgtc ggaatcgaac gtatctctca gcgcaattct gccgatcttt
     3001 tcctttagca gctccgcacg tgataatagc agttgacttg cagcagcgcc ttctttggca
     3061 gctgccgtat ttttttctac aacggcggaa attgcgccga catcctgcgt catccgctgc
     3121 atggaagcag tctgtttttc cagatcctgt cgaacctcct gaatggtttt gctgacggtt
     3181 tcaatttcct taagagattc gctcagggaa tttgcggtat cggccgcgat cgaagaactc
     3241 tcctccacgc ttcggatgga agcatcaatc atcaaggcgg tctgctttgc cgcctccgcg
     3301 gattgccccg ctaagttccg gacctcggcg gcgacgacgg caaatccttt tcctgctccg
     3361 cctgcccggg cggcttccac ggccgcgttc agcgcaagca gattggtccg ctgggcgatg
     3421 tcggaaatca tgcggttgac cttctgaatc tctcccgagg actgccgggt cttttcctcg
     3481 gcctttaaaa gctgatccat ttggttatcg ccgtcgatga ttctctcaaa aattcgcccg
     3541 atggagccga ccgcgtggtc catttcgcga gtggtgttct cggcaatttc gttgacgcgt
     3601 ttgatggaag aatataactg attcaccgta agagtctg
//
LOCUS       DATALY010000174         4665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_383061,
            whole genome shotgun sequence.
ACCESSION   DATALY010000174 DATALY010000000
VERSION     DATALY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383061"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VHR42_05105"
     CDS             <1..321
                     /locus_tag="VHR42_05105"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HEX3026587.1"
                     /translation="MPVIVAEYGTVEKLVPPMLRPAFKKGKIDHPDIFDDAGWKGRFR
                     MIPFGSVGNSGGLLPAFRPDRIIVRGTGRETKISEILIAVSNGKLSSDGRYSVLLNTH
                     LFRQ"
     gene            431..1129
                     /gene="sigE"
                     /locus_tag="VHR42_05110"
     CDS             431..1129
                     /gene="sigE"
                     /locus_tag="VHR42_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HEX3026588.1"
                     /translation="MEAVYARLILKFRLFGHRIYYINGPETLPPPLSTEEEGKCLRRL
                     EAGDLNAKNMLIVHNLRLVVYIAKKFESTGIGVEDLVSIGTIGLIKAVNTFCPSRNIK
                     LATYASRCIENEILMYLRKSSNLKNEVSIDEPLNIDWDGNELLLSDVLGTDNDIVHHN
                     LERDVEKNLLDTAIAHLSKRERTIMQMRFGLGDGVERTQKEVADSLGISQSYISRLEK
                     RIILRLKKEIERVS"
     gene            1353..2123
                     /gene="sigG"
                     /locus_tag="VHR42_05115"
     CDS             1353..2123
                     /gene="sigG"
                     /locus_tag="VHR42_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HEX3026589.1"
                     /translation="MQINKVEICGVNTSKLKILSEAEKGRLLRLVKKGDASAREQLIN
                     GNLRLVLSVIQRFTNRGENLDDLFQVGCIGLIKAIDNFDISQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSMRDIAYKAMQVKEKLINKSQREPTIEELASEMQMPKEEIVLAL
                     ESVVEPVSLYEPVYSDGGDTIYVMDQIGDKNQDEDWLDEITLKEAICKLSDREKKILS
                     LRFFEGHTQMEVASEIGISQAQVSRLEKGALSKIKKQI"
     gene            2293..2577
                     /locus_tag="VHR42_05120"
     CDS             2293..2577
                     /locus_tag="VHR42_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HEX3026590.1"
                     /translation="MCRIEELCRKDVINTKDGCRLGNVCDVEIDVANGMVTALVIYGR
                     SKFFGLFGRCEDIVIPWCSIQKIGADTILVCFDIQYRRKFKGRSFFDFFR"
     gene            2713..3474
                     /gene="rpsB"
                     /locus_tag="VHR42_05125"
     CDS             2713..3474
                     /gene="rpsB"
                     /locus_tag="VHR42_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX3026591.1"
                     /translation="MSSVVTMKQLLEAGVHFGHQTRRWNPKMAPFIFTERNGIYIIDL
                     QKTVKKLEEAYMFVRDIAASGQNVLFVGTKKQAQDSVRDEATRAGAYFVNARWLGGML
                     TNFRTIRHRIDRLKQLRKMEEDGTFDLLPKKEVAKHKLEIEKLEKTLGGIKDMKRLPA
                     ALFIVDPRKEHIAVAEARKLGIPIVAIVDTNCDPDEVDYIIPGNDDAIRAVKLISSIM
                     SNAILEGRQGEQTEETAPEGAVEAKIAAPAATVAE"
     gene            3637..4545
                     /gene="tsf"
                     /locus_tag="VHR42_05130"
     CDS             3637..4545
                     /gene="tsf"
                     /locus_tag="VHR42_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX3026592.1"
                     /translation="MAFTAKDVQALRETTGCGMMDCKKALQSSDGDIEKAIEFLREKG
                     MAAAAKKAGRIAAEGVVYADVNEADRVGVILEVNAETDFVAKNDGFKAFVKTCAETIE
                     KQNPADVEALLTLKAEGSNMSVAELLREKVQTIGENIKIRRFTRLEGDLVAYVHGADR
                     IGVLVKYDTDLGAKPGFKEYAKDIAMQVAAANPQYLDRSSVPSETVEEEKKVLTAQAL
                     NEGKPQNIAEKMVMGRINKFYKDTCLVDQDFIKDGDLSVAKYTEKKAKELGGTIKIVQ
                     FIRYEKGEGLQKREDNFAEEIANMVK"
BASE COUNT         1360 a          954 c         1220 g         1131 t
ORIGIN      
        1 atgccagtga tcgttgcgga atacggtacg gttgaaaaat tagttccgcc aatgttgagg
       61 ccggctttta aaaagggaaa gattgatcat cccgatattt ttgatgacgc ggggtggaag
      121 ggacgcttcc gaatgattcc attcggttcg gtaggaaatt ccggagggct tcttccggcg
      181 tttcggcccg accggattat tgttcgggga acgggcaggg aaacgaaaat ttcagaaatt
      241 cttattgctg tcagcaacgg aaagctctca tccgacggga gatactccgt gcttctgaac
      301 acgcacctct tcagacagtg attgactggg ttttaacagt gggagcaatt tgttgacata
      361 gaaagattat gactaacaaa caggagatgg tggcatgttc ggccgtttag tgaacgtggt
      421 tttatcattt atggaagcgg tttacgccag gttgatttta aaattccgtt tatttggtca
      481 tcggatttat tatatcaacg ggccggagac gcttccaccg ccactgtcga cggaagaaga
      541 gggcaagtgc ctgcggcggc ttgaagccgg tgatcttaac gcaaaaaaca tgttgatcgt
      601 tcacaattta aggctggttg tctatatcgc aaaaaagttt gaaagtacgg gcatcggcgt
      661 ggaggacctc gtctcaatcg gaaccatcgg tctgattaag gccgtcaata cgttttgccc
      721 gtcaagaaac attaagctgg ccacctacgc ttcccggtgt atcgagaatg aaattctcat
      781 gtatcttaga aaaagctcca acttaaaaaa tgaagtgtcg atagacgaac ccctgaatat
      841 tgactgggat gggaatgaac tccttctttc ggatgtattg ggaaccgata atgatattgt
      901 gcaccataat cttgaacggg atgtggagaa gaatcttctt gataccgcta tcgcgcatct
      961 ttccaaacgg gaaaggacaa ttatgcagat gcggtttggg ttgggcgatg gtgttgagag
     1021 aacgcagaaa gaggtcgcgg attcacttgg aatttcccag tcttacatat ccagactgga
     1081 aaaaagaatt attctcagat tgaaaaagga aatcgaacgt gttagttaaa atagagttca
     1141 ggcggcccgt aaggagcgtg aaagcccggc gggccgccgt tgatttttcc gcttgaaatt
     1201 gcaaagaaaa gcttcccgat tctcttgtta aaatttcgtt cattcggtat gaaaacacct
     1261 caacccggta atactgaaaa tggaaataag ttgccagaga gttcgtttga aatggcagcg
     1321 taccgttttg ccagtaaagg ggaggggcca atatgcaaat caataaagtt gaaatttgcg
     1381 gcgttaatac atccaaacta aaaatccttt ccgaagccga aaaaggcaga ctgctcaggc
     1441 tggtcaaaaa aggggacgcg tcggcgagag agcagctgat caatggtaat ctcaggcttg
     1501 tgttaagcgt gatacaaaga tttacaaaca ggggagaaaa tctggacgac ctttttcagg
     1561 tcgggtgtat tggtttaatt aaagcaattg ataattttga tataagccag aatgttcgat
     1621 tttcaaccta tgcggtgccg atgatcattg gggagatcag acgttatctt agagacaaca
     1681 atcccattcg tgtcagccgc tccatgaggg atatcgccta caaagccatg caggttaagg
     1741 aaaagctgat caataaatcc cagcgggagc cgaccatcga ggaactggca agcgaaatgc
     1801 aaatgccgaa agaagaaatc gtgctggctc tcgaatcggt ggttgaaccg gtgtcgcttt
     1861 acgagccggt ctattccgac ggaggcgaca ctatctatgt catggatcag atcggcgata
     1921 aaaatcagga tgaagactgg ctggatgaaa tcacgctgaa agaagcaatc tgcaagctct
     1981 cagaccgcga aaagaaaatc ctttctcttc ggttttttga ggggcatacc caaatggagg
     2041 tcgcatccga aatcggaatt tcccaggctc aggtttccag actggaaaaa ggtgctttat
     2101 caaaaatcaa aaaacaaatt tgactcaaag gatatcattc ttcataatat ggaaatggta
     2161 tcctttttaa tcattctccg gttctgtcag aaacggagaa tgattgaccg tgcgtctgaa
     2221 taagatagaa taccgtaaca gtaactaaaa ttatgtcagc ctcaagcaaa gctgaattgg
     2281 gggtgctggt ctgtgtgccg gattgaggag ctttgccgca aggatgtcat caatacaaag
     2341 gacggctgcc gcctgggaaa tgtctgcgat gtggaaattg acgtagccaa cggaatggtt
     2401 accgcgctgg tcatttacgg caggagcaaa ttctttggcc tttttggccg atgcgaagac
     2461 atagtaatcc cgtggtgcag cattcagaaa atcggtgcgg atacgattct ggtctgcttt
     2521 gatattcaat acaggcgaaa atttaaagga agaagttttt ttgacttttt ccggtaagct
     2581 tgcaattatt gcgaaaatgt gttataatct gcaacgctat aacgcacgca tcgttgttgt
     2641 cgggcggtgc cttttttaaa ggatcccggc aagagaatcg atgcggagga gaaaaccgaa
     2701 ggaggaaaat tcatgtcatc agtagtaact atgaagcagc tcctggaagc cggcgttcat
     2761 ttcggtcatc agaccagaag gtggaacccc aaaatggctc cctttatctt tacggagcgc
     2821 aacggcatct atatcattga cctgcagaaa acggtcaaaa agctcgagga agcttacatg
     2881 tttgtccgtg acatcgcggc aagcggccag aacgtacttt ttgtcggaac caaaaaacag
     2941 gctcaggatt ccgtaaggga tgaggcaact cgcgccggag catattttgt caacgcccgc
     3001 tggcttggcg gcatgctcac caacttccgc accatccgtc acagaatcga caggctcaaa
     3061 cagcttcgta aaatggagga agacggtaca tttgacctgc ttccgaaaaa ggaagttgcg
     3121 aagcacaagc tggagattga aaaacttgag aaaacccttg gcggcatcaa ggatatgaag
     3181 aggcttcccg ccgctttgtt tattgttgat ccccgcaagg aacatatcgc ggttgcagaa
     3241 gcccgcaagc tgggtattcc gatcgttgca attgtcgata ctaactgcga tcctgatgaa
     3301 gttgattata tcatccccgg aaacgatgac gcaatccgtg cggtaaaact gatctcttcc
     3361 attatgtcca atgccatttt ggaaggcaga cagggcgagc agactgagga aacggctcct
     3421 gagggcgccg tggaggcaaa gattgcggca ccggctgcca cagtggcaga ataacaacaa
     3481 attgtccgcg ctgccggtaa cggggcaaaa ggcgcatggg ctgatgatgc ccgcgcaagg
     3541 ttgctgcctt gccgcgggca ttttaattaa gaaatcgggt ttgtttgccc gcaataagat
     3601 gaaaaaagac tatgttgtta aacaggaggt ttcaaaatgg cttttacagc aaaagacgtt
     3661 caggcattaa gagagacaac cggatgcggt atgatggatt gcaaaaaggc tcttcagagt
     3721 tccgatggag atattgaaaa agcaattgaa tttttgcgtg aaaagggcat ggccgccgct
     3781 gcgaaaaaag cgggcaggat tgctgctgaa ggcgtcgtat atgcggatgt caatgaagcg
     3841 gatcgggtcg gagtcattct ggaagtcaac gccgagaccg attttgtcgc caaaaacgac
     3901 ggttttaaag cttttgtcaa aacctgcgcg gaaacaatcg aaaaacagaa cccggctgat
     3961 gtcgaagctc tgcttacact gaaagcggaa ggcagcaata tgagtgttgc cgagctgctc
     4021 agggagaaag tccagacgat cggcgaaaac atcaaaatcc gccgttttac ccgtctggaa
     4081 ggcgatcttg tcgcttatgt ccacggtgcg gaccgaatcg gagttttggt caaatatgat
     4141 accgatcttg gagcaaaacc cggatttaag gaatatgcaa aagatatcgc catgcaggtt
     4201 gcagctgcga atccccagta tcttgaccgg tcttccgttc cttccgaaac cgttgaggaa
     4261 gaaaagaagg ttttgaccgc tcaggcgctg aacgagggca agcctcagaa cattgccgaa
     4321 aagatggtca tgggccgcat caacaagttc tataaagata cttgcctggt tgatcaggat
     4381 ttcattaagg acggagacct gtccgtagcg aaatataccg agaaaaaggc caaggagctt
     4441 ggcggaacga tcaaaatcgt gcagtttata cgctatgaaa aaggcgaagg tttgcagaag
     4501 cgggaagata atttcgctga agaaatcgcc aacatggtaa aataagaaaa aaagccgtcg
     4561 tcagacggct ttttttctgg gaatttacat gatatggtta aattataaag gcgaaagaat
     4621 tttttgattc ggtcagcgga ggaaacatca gcctgattat ttttc
//
LOCUS       DATALY010000175         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_813775,
            whole genome shotgun sequence.
ACCESSION   DATALY010000175 DATALY010000000
VERSION     DATALY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813775"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VHR42_05135"
     CDS             complement(<1..1295)
                     /locus_tag="VHR42_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3026593.1"
                     /translation="MIAIYARQSADREDSISTDSQIEACQYEARGEEYRRYVDKGYSG
                     KNTDRPCFKELVTDIKKGEISKVIVYRLDRISRSILDFSNMMVLFSKYQVEFVSSTEK
                     FDTSTPMGRAMLNICMVFAQLERETIQQRVTDAYYSRSEKGFYMGGRVPYGFRLKEGA
                     INGKRTKTYIPYEEEADQIRLMYDLYSEPNTSYGDIVAYFQAHGITCRGKPWERARIA
                     DHLKNPIYAQADLDVYEFFQQQGTVIESPQEDFVGTNGCYYYKGRDTNNRKLADLKDN
                     RLVLAPHEGFIPSDIWLKCRTKCLQNKQIQPARKVVNTWLAGLVKCANCHYALTFKKY
                     NTRRSRYLLCSHKLNSKACEGAGTIYADEFEAMIYQEMVKKLEEFPVLAKNDEPVQEN
                     PKAAALRIELNKVKQEIESLMDKVALANATLMEFINRRI"
     gene            complement(1777..2373)
                     /locus_tag="VHR42_05140"
     CDS             complement(1777..2373)
                     /locus_tag="VHR42_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3026594.1"
                     /translation="MNLSYGYARISSRDQSLSRQIVALHDYGLDDRHIITDKASGKDF
                     AREGYQTLKTQLLRAGDTLVIKELDRLGRNYTQIKIEWQELLQMGVEIVVLDTPMLNT
                     ANKSDLEKKLIANIVFELLAYMSEKERLRIHARQAEGIAAAKAQGKRLGRPMIPMPPD
                     FKEQYQKVLNHEISATQLMKTLGLKRNVFYRCIHELQK"
BASE COUNT          721 a          731 c          640 g          819 t
ORIGIN      
        1 taaatgcgcc ggttaataaa ttccataagg gttgcatttg ccagcgcgac tttgtccatt
       61 agggattcaa tttcctgctt aaccttgttt agttcaattc gaagtgccgc agctttcgga
      121 ttttcctgaa ccggttcatc attcttggcc aataccggga attcttccag ctttttaacc
      181 atttcctgat agatcatggc ttcgaactcg tcggcataga ttgttccggc cccttcacac
      241 gccttgctat tcaatttgtg gctgcaaagc aggtagcgtg accggcgtgt attgtatttt
      301 ttaaacgtca atgcgtaatg gcagtttgca catttcacaa gaccagccag ccatgtgttg
      361 actacttttc gcgcgggttg aatttgctta ttctgtagac atttcgtacg gcattttagc
      421 catatatccg atgggataaa cccttcgtgg ggagccagaa caagccgatt atcctttaaa
      481 tctgcgagct tgcggttatt tgtgtctcgc cccttgtaat agtaacagcc gttcgtcccg
      541 acgaaatcct cctgagggga ttcaatcaca gtaccttgct gctgaaaaaa ttcatagaca
      601 tcaagatctg cctgagcgta aatagggttc tttaaatgat cggcaattcg ggcccgttcc
      661 catggctttc cccgacaggt aattccgtgc gcctgaaaat aggcaacgat atcgccgtaa
      721 gatgtatttg gctcggaata caggtcatac atcagccgga tctgatctgc ttcctcttcg
      781 taaggaatgt atgtctttgt ccgcttcccg ttgattgccc cttcttttag acgaaatccg
      841 tatggaacgc gtccacccat ataaaaccct ttttcgctgc gggagtaata agcgtcggtg
      901 acgcgttgct gaatggtttc gcgctccaat tgtgcaaaaa ccatgcagat attcaacata
      961 gcgcggccca taggagtgga agtgtcaaat ttttctgtag atgatacaaa ctcaacctga
     1021 tatttagaga acaaaaccat catattagag aaatccaaga tagaacggct gatacgatca
     1081 agccggtaaa caattacttt agaaatctcc ccttttttta tatcggttac aagctcttta
     1141 aaacacggac ggtctgtgtt cttcccgcta tatcccttat caacatacct gcggtactct
     1201 tcgcctctgg cttcatattg acaggcttca atctggctgt cagtggatat actatcttca
     1261 cggtcagcgg attgccgggc ataaattgca atcattgttt catccctctc tttcatgaaa
     1321 aagcagagtc tgcggttggt tgagtataac tctgcggccc tgctttctca agttattttg
     1381 acatcagcga tatttggaga aaaccccata gagtgcttga ttaatccctg cttttacttt
     1441 tttccgttcc tgcgccgtat acaccggtgt cagattccga atagtgacgg tcttacccct
     1501 gcaggaaatt tgatggcttt cttctcgata tttttttccg tttgtcatgg agcatcagcc
     1561 cctttgcgta ctaccacttt atgacgaacg gatgtcccaa aatgtatgtc cgagcgtctg
     1621 catgacggta ggctttcgta aaaaattacg attgctgagc tctataacca gcctcctgat
     1681 gcaactcctt tccaaacgga aaaactgcag atcgtttgta cactgcggta aaaccagcga
     1741 atatgaaaag ggagagggcc gaaaccctct cccaaactat ttctgaagtt catgtataca
     1801 tcgatagaaa acgtttctct tcaagccaag agttttcatt aattgagtgg cactaatctc
     1861 atggttcaaa actttctgat attgttcctt aaaatcaggc ggcataggta tcataggtct
     1921 gccgagacgc ttaccctgtg cctttgctgc cgcaatccct tctgcctgac gagcgtgtat
     1981 gcgaagcctt tccttttcgc tcatgtaagc caaaagctca aacacgatat ttgcaatcag
     2041 ctttttctcc aaatcggatt tattggccgt attgagcatt ggcgtatcca atacaacaat
     2101 ctcaaccccc atttgcagaa gctcctgcca ctctattttg atttgtgtgt aatttctgcc
     2161 caatcggtcg agctctttaa tgacaagagt atctcctgcc cgtagaagct gagtttttag
     2221 cgtctggtag ccttcccggg caaaatcttt tccgctggcc ttatcggtga taatatgtct
     2281 atcatccaag ccgtagtcat gtagggcaac tatttgtcgg ctaagacttt gatctcgact
     2341 tgagattctg gcgtaaccat agcttaaatt caatttaatc gcctccctaa gttggttaag
     2401 gtgctcatat ttttcaaatg ttgggatatt ccccacaaag gctttacgaa acaggaaaaa
     2461 cggcagaatt gaaccgttgc tttaagcaca ctttcacgga tacccagccc cgccaagagc
     2521 cacaagcagc gttcaagcct gtacgccgat ttgtggctcg aaagccgcta gcggctcccg
     2581 tgatacccgg aacaaccttg agcaaaacgt cgagcccgac agcgccagcg ttgaggacga
     2641 tacccgtagc cggggaaagc ggcgaaccgg gcagggaacg gggtcgaaat cgcgcccagc
     2701 ggtaccgcgc agctttgcca cttgtaaggc gctgtgtggc tggaaaacca aaagcggccc
     2761 cgtatacccc ggagccgcct tacacaaacg ttacccccgg cgacgcaagc gttggagcaa
     2821 atgccttaat accggaaaac cgggaacggg cgcgggcccg cccttcactc gaataccgtc
     2881 gcatatccag ttcgatccgg gggatgagcg g
//
LOCUS       DATALY010000176         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_38850,
            whole genome shotgun sequence.
ACCESSION   DATALY010000176 DATALY010000000
VERSION     DATALY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38850"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(635..1318)
                     /gene="deoC"
                     /locus_tag="VHR42_05145"
     CDS             complement(635..1318)
                     /gene="deoC"
                     /locus_tag="VHR42_05145"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX3026595.1"
                     /translation="MKTLSVSALAKYLDHVGDWNIPLEQYCETAVKYGVRCCAVNIEE
                     VKFCKDYLKGTDILVSGAVAFPGGSGTIQGKLADVGYAMENGADEVDYVINTHNVKNH
                     DWDKVEEEITAIAELCSKNGVADKVIFENCLYNPEEKAKLCEIAVKAGPAFIKTSTGK
                     SFGGATPEDVRLMKKIAGEKCKIKAAGGIRNYYTALAMIEAGADRLGTSSAVKILEGY
                     AAYLESMQK"
     gene            complement(1315..2250)
                     /locus_tag="VHR42_05150"
     CDS             complement(1315..2250)
                     /locus_tag="VHR42_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3026596.1"
                     /translation="MRVLCIGDNDSSFYRWMGIMFPGGNCPNVAAYCRLNGHESGYIG
                     VIAADRVGNHFLSALQSLKVDVSRIRVCEGSTTFTDVTLNNADRVFGEYRNDIQKSNP
                     IHLGEDDLAYIRSFDLAHSSIYSALDGNVLPRLKENGIRVSYDFSDSWTGRNIETICP
                     NLSYAFFSCGHLDQKQTKLTLVEAVRYGAAVAVGTRGVGGAHLFDGRRFYYQRAYPAD
                     AVDTIGAGDAFIARFLLSYEEGRKIREKCDGPHAERSAFRQEDLDENRSVLTRCAMSQ
                     AALFAAGVCQHYGPIGMGMKMDMQLNSIDGKDALL"
     gene            2637..3083
                     /locus_tag="VHR42_05155"
     CDS             2637..3083
                     /locus_tag="VHR42_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3026597.1"
                     /translation="MDSIDFQIIDLLQQNARTSLKKMAETVSLTPPAVSERIRKLEAS
                     GVITGYRAVINPDKVGFAIRAVINVTISREIQNDFVRFAKTCKNIIKCHRVTGEYSMS
                     LEAVFHDMTELDTIVGKIQRYGDTQTLLILSSAISFRPLVPHLEKD"
     gene            complement(3143..>4585)
                     /locus_tag="VHR42_05160"
     CDS             complement(3143..>4585)
                     /locus_tag="VHR42_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026598.1"
                     /translation="AEIFNARQAGKDVSMSRQVKTVPELAPLRLINAAQRHVVKNLDW
                     YTQAAVLITDRFYRLLLDKNLPVIGINHRLKSPESLKEKILRKKLYKGSQTPEQIIRK
                     ITDMIGVMIECEFISDEEKIFSCIRDTFPLSEDGVFFYNPQFPDMTVDFGTPQPVKQK
                     NGYELYKMDCRYADESGEILFELQIKSLVNSFWCEVEHNIVYKNNHYISSDDYINQML
                     SAVRINLAGLDRILQLISDRIGAMTAYNVVRDLRLNTSLTKQLVSDLINAKMVESVGF
                     TMEAKRIRDLLAWYILEKLGPSDENSSAFFQLSGRFKELHQTEFRFDRQIELEYDLSS
                     KDIFSRTVGGKMLQLMNSDFEWHVFFIMIFAIYAEQEPEEVFGEFIDTLSRIFCGGSY
                     FQGLFDRLPPEQAQTVSDEAGMFLGEALCGIGDTGILPVEVYHKGFEEVDGFLQYTLN
                     CLDNLEEWVQKRSAVQAVINKSLRKSCLLK"
BASE COUNT         1164 a         1191 c          968 g         1262 t
ORIGIN      
        1 cacatgtgtc gcgacaagcc cataataaaa tttctgccca attttaaaat aaaaagacgc
       61 ctgtattaat ccggccgaat taatacaggc gtctttgccc aacaactatt tattttcata
      121 atttctattt ttattataac ataagctttt agaaattgca ataatttaat tttaaaaatt
      181 cattttatac agtgatgtgt tttttatcct tttccggcgt ccgattcatt attgcttaaa
      241 atggcttaat atagcagttg ttcgttgtat tacagttgat gtgccacaaa ctgtctaaga
      301 gaatcaatat gaattatgaa tattatgtta cttcatattt cattttcagg caaatactct
      361 ttcagtggct tacctcaagt gctgaatcat actatggaca ggaggatgat aaaagaccgc
      421 ccgtccgtaa tactttgcta ctatccgccc cacagcaggg tgctgttgaa acataaaaaa
      481 agacgctccg gaacaaaaat tccaaagcgt ctttgctttc tgaaaacaac aactcattat
      541 cctatttcaa tgtgattcaa taagtactca tttcattgtt ttcattatac gttggttttc
      601 caatcttgtc aacagacttt ttgccgctaa atatttattt ttgcatactc tctaaataag
      661 ccgcataccc ctcaagaatc tttactgccg aactggtgcc gagccggtcg gcaccggctt
      721 cgatcattgc cagagccgta taataattgc ggattcctcc cgctgctttg atcttacatt
      781 tttcccccgc aatttttttc atcagcctga cgtcttcggg cgtcgccccg ccaaagctct
      841 tgcccgtgga tgtttttata aaggccgggc ctgctttgac ggcaatctca cagagttttg
      901 ctttttcttc aggattataa aggcaatttt cgaaaataac cttgtcggcc accccgtttt
      961 tactgcaaag ttccgcgatg gcggtgattt cctcctcgac cttatcccaa tcatgatttt
     1021 tcacattatg cgtgttgatc acataatcaa cctcatccgc cccgttttcc attgcgtagc
     1081 cgacgtccgc caattttccc tgaattgttc ctgatccccc ggggaaagca acggctccgc
     1141 tgaccagaat gtcggtgcct ttaaggtaat ctttgcagaa tttcacctct tcaatattaa
     1201 ccgcgcagca cctgacccca tatttcaccg cggtttcaca gtactgctca agcgggatgt
     1261 tccaatcccc cacatggtcg aggtactttg caagcgcact taccgataac gttttcataa
     1321 cagcgcatcc tttccgtcga ttgaatttaa ttgcatatcc atcttcatcc ccataccaat
     1381 cggaccgtag tgctggcaga ccccggccgc gaaaagcgcc gcctgtgaca tcgcgcatcg
     1441 cgtcagaaca cttctgtttt catccagatc ttcctgacgg aaagccgatc gttccgcatg
     1501 gggcccgtcg catttttccc ggatcttacg gccctcttca taactcagaa gaaaacgcgc
     1561 aataaaagcg tcgcccgccc cgatcgtatc aaccgcgtcg gcgggatacg ccctctgata
     1621 atagaatctt ctgccgtcaa acaggtgcgc tccgccgacg cctctggttc ccaccgcaac
     1681 ggctgcacca tagcggacag cctcaacaag cgtcagtttc gtctgcttct gatccagatg
     1741 tccgcaggaa aaaaatgcat agctgagatt cgggcaaatc gtttcgatat tgcggccggt
     1801 ccaactgtct gaaaaatcat aggagaccct gataccgttt tctttaagcc ttggcagaac
     1861 attgccgtcc agagcactgt aaatactgct gtgcgccaaa tcgaaactcc ggatataggc
     1921 aagatcatcc tcacccagat ggatcgggtt gctcttttga atatcgttgc ggtattcccc
     1981 gaaaacccgg tcggcgttgt tgagcgtcac atcggtgaat gtggtcgagc cttcgcagac
     2041 gcgtattctt gacacatcga cctttaaaga ctgcagggcc gaaagaaaat gattgcccac
     2101 ccggtcggcg gctatgaccc cgatataccc cgattcatgc ccgttgagcc tgcagtatgc
     2161 ggcgacattc gggcagtttc cgcccggaaa cattattccc atccacctgt aaaagctgct
     2221 gtcgttatcc ccgatacata ggactctcat attgtctcac ccgctttgtt caggatgtgt
     2281 tctctctcta tctatatggc tcgtcccgga tgcatattcg taatttgact cgatccgtct
     2341 gtttttcttt cgagcaaaga gagggaaacg gatgaaattt tccgatcctc ccgattgcct
     2401 gtgaacgccg catgccggat gcggaacacc atttggattt tttcgcccgc gcagttgaat
     2461 tttttgaaaa ttaggattac aatttagatg gaacaacaag agggcctgat tgttttagtc
     2521 aggccctgaa atctgccaga atataccttg ctttttgttt tttgacccgg gcatgcagta
     2581 agaaaatttg agcttcatcc tcaagatgac tatgagaaac cacgggggct ttcaatatgg
     2641 attcgattga tttccaaata atagacctgt tgcagcaaaa cgcgaggact tctctcaaaa
     2701 agatggcgga aaccgtatcc ctcacgccgc ctgccgtttc tgaacgtatt cggaaacttg
     2761 aggcctcagg cgttataacg ggttaccgcg ccgtcattaa tccggacaaa gtagggtttg
     2821 cgatccgggc agtcatcaac gtcacaatca gccgggaaat ccaaaacgat tttgttcgtt
     2881 tcgccaaaac ctgtaaaaat atcatcaaat gccaccgcgt cacgggggag tattccatga
     2941 gccttgaagc ggtatttcac gacatgacgg agctggatac catcgtggga aaaatccagc
     3001 gttacggaga cacgcagacg ctgctgatcc tgtcaagcgc catcagcttt cgtccgcttg
     3061 ttccccacct ggaaaaagat taacttccat ttcatcaatc aaagccatac agccgcagcc
     3121 ccgtttgggg ctgcggctgt atttatttca aaaggcagct cttcctcaga cttttattaa
     3181 tcacagcctg aaccgcgctt cgtttctgaa cccattcctc cagattatcc agacagttga
     3241 gggtatattg caagaagccg tctacttcct caaaaccttt gtggtagact tccaccggaa
     3301 gaatcccggt gtcgccgatt ccgcagagtg cttcgccgag aaacataccc gcctcatcgg
     3361 aaacagtctg agcctgctcc ggaggcaaac ggtcaaacag cccctgaaaa tagctcccgc
     3421 cgcagaaaat ccgactcagg gtatcgatga actccccaaa aacttcttcc ggctcctgct
     3481 ccgcataaat cgcaaagatc atgataaaaa agacatgcca ctcaaagtcg gaattcatca
     3541 gctgaagcat ctttcctccc accgtccggc tgaaaatgtc tttggaggaa agatcatatt
     3601 ccagctcaat ctgccggtcg aatcggaact cggtctgatg cagttcttta aaccggccgg
     3661 acagctggaa aaaggcggaa gagttttcat cggacggccc gagcttctca agaatatacc
     3721 aggcgagcag atcacggatg cgtttggctt ccatcgtgaa accgaccgac tcaaccattt
     3781 ttgcattaat caggtcgctg accagctgtt tggtcaggct ggtgttcagt cggagatccc
     3841 gaacaacatt ataggcagtc atggcgccga tacggtcgct gatcagctgc aggattctgt
     3901 caagcccggc caagttgatt cggacagcgg aaagcatctg gttgatataa tcatccgaag
     3961 aaatataatg gttattttta tagacgatat tatgttccac ttcgcaccag aaggaattca
     4021 ccaatgattt gatctggagc tcaaacagga tttctccgct ttcatcggca tagcggcaat
     4081 ccattttata aagctcatat ccattttttt gcttaacagg ctgcggcgtt ccaaaatcga
     4141 ccgtcatatc ggggaactgt gggttataaa agaagacacc gtcctcggag agagggaacg
     4201 tatccctgat gcaggaaaag attttctctt cgtcgctgat aaattcgcat tcgatcatca
     4261 caccgatcat atcggttatt ttcctgatga tctgctccgg agtctggctg cccttataca
     4321 gtttttttct caggatcttt tctttcaggc tttccgggga tttcagccgg tgattgatgc
     4381 cgataacggg cagattttta tccagaagca aacggtaaaa ccggtcggta attaaaaccg
     4441 ccgcctgagt ataccagtcc agatttttga ccacatgcct ttgggcggca ttgattaaac
     4501 gaagcggcgc gagctccgga actgttttga cttgcctgct cattgaaaca tcctttcccg
     4561 cctgccttgc gttaaaaatt tcagc
//
LOCUS       DATALY010000177         4832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_728035,
            whole genome shotgun sequence.
ACCESSION   DATALY010000177 DATALY010000000
VERSION     DATALY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728035"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1253)
                     /locus_tag="VHR42_05165"
     CDS             complement(18..1253)
                     /locus_tag="VHR42_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3026599.1"
                     /translation="MKLSGRRIVAMALLVIFILTGFSVRLARYQIVEGADYREQSQKV
                     TIQSSEIEAPRGEILDRYGRVLASNKVGYSIYLQKAFFPSTKQPVKQNNVINSICKIL
                     SQKGESWNDSLPISKTAPYCFNAKNTSDLKKLRSLLNVSDKVGASDIMKKLVSRYHLE
                     GNSSADQRTLAGIRYEMEIRDFTYSNPYEFASSVSIDTVTKIKERSTSLPGVEIREVP
                     TRVYPDGTIAPHVIGTVGPIYAEQYAALKKKGYSMNDTVGKDGIEYTMESYLRGTKGE
                     QTIEMDSMGNVTSTNITTKANPGDNVVLTIDSDLQKLVQSSLQSTIQNIASTAKSVKW
                     GSQAKSGAAVVLDIKTGEVLAMANYPSYDLNTYKKDYSALSNDSANPLLNRCVTGSYR
                     PGSTFKPITAISGPTVPIT"
     gene            complement(1301..1804)
                     /gene="mreD"
                     /locus_tag="VHR42_05170"
     CDS             complement(1301..1804)
                     /gene="mreD"
                     /locus_tag="VHR42_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX3026600.1"
                     /translation="MRNYNWVKWLVYAIVIFFVCLIQTNPEFPRILQNGPLLAIPAVI
                     TLSMFEGENAGAAYGIAAGLIWDVQTGQVFGYNAFFLLIAGIMVGLMIKYLFSRSLFT
                     DIFLTLGISFGFELLSWFFFYYMTGRGEFLFFLVHVILPTVFYTALFTIPIYYGVRRI
                     NRKLTAA"
     gene            complement(1805..2686)
                     /gene="mreC"
                     /locus_tag="VHR42_05175"
     CDS             complement(1805..2686)
                     /gene="mreC"
                     /locus_tag="VHR42_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX3026601.1"
                     /translation="MRDFFRSVWFKIFAVIALILFGLMLRTGMSGGFASIPANVMGFV
                     LTPLQKVSAQVSGAFSGAVSTVASYGSVKKENEQLKKEVSSLNSKLVDYNQMKNENSQ
                     YKEYLGIRDDNQDFKWEPAMVIARDPGQWFSVFTIDKGSLNGIGVNDPVITSDGLVGK
                     VTQVMSTSSIVTTILDPSVSVGALVSQTNDPCITQGDKELGAKGRMKVSYLAKESTAA
                     QGDIVITSGIGGIFPKKLKIGIVEEIRTETSGLSLYAVVKPMVDPSTVKNVFVITSFQ
                     GKAGDATKSSSSQSGGK"
     gene            complement(2865..3881)
                     /locus_tag="VHR42_05180"
     CDS             complement(2865..3881)
                     /locus_tag="VHR42_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3026602.1"
                     /translation="MFSRDIGIDLGTANTLIYMKGKGIVLREPSVVAVNVKNDRVLAV
                     GNEAKQMIGRTPGSIVAIRPLKDGVIADFDITSSMLKSFIKKVISGGAFSKPRIIICI
                     PSGVTEVERRAVDEAAYQAGAKDTYLIEEPMAAAIGAGLPVDEATGSMVVDIGGGTAE
                     VAVISLSGIVTSRSVRVAGDEFDDAIINFVKKKYNLLIGERTAEGIKINIGSAFPYPE
                     EGSVEIKGRNLLDGLPSNITITSDEVREALADPVSMVVDAIKSTLERTPPELSADIID
                     RGIMLTGGGALLRGLDELIAKETGIPVNIAERPLDCVVDGTGKMLEDMNGSLANVLFN
                     ARHK"
     gene            complement(4244..4816)
                     /locus_tag="VHR42_05185"
     CDS             complement(4244..4816)
                     /locus_tag="VHR42_05185"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019391325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX3026603.1"
                     /translation="MKLILASSSPRREEILRNAGYEFEVIPSRVDENLPEPLPPEETV
                     TRLAVLKAGEIFSQYPDAVVLGADTVVSVGGKILGKPKDEQDAAGMLEKLSGRTHEVF
                     SGVALMADGRRESFAEKSQVCFYPLGQELIKRYTATGEPLDKAGAYGIQGFGSLLVRE
                     ITGDYYNVMGLPVARLGRALENFGITPLWR"
BASE COUNT         1218 a         1319 c         1081 g         1214 t
ORIGIN      
        1 gatggaacga ttgcccctca tgtgattggg acagtaggcc cggatatggc cgtgatgggc
       61 ttgaaagtcg aaccggggcg gtaagacccg gtgacacagc ggttgagcag cggattggcc
      121 gaatcgttgc tcagcgccga atagtctttt ttgtaagtat tcaggtcata ggacggatag
      181 ttggccatgg caagcacttc accggtcttg atatccagaa ccaccgccgc gcctgatttt
      241 gcctgcgagc cccattttac gctcttcgcc gtcgacgcga tattctggat cgtagactgc
      301 agggaactct gaaccaattt ctgaagatca gaatcgatgg tcagcacgac attatcaccg
      361 ggatttgcct tagtggtaat attagtgctg gtaacattgc ccatactgtc catttcaatg
      421 gtctgttcac cctttgtgcc ccgcagatag gattccatgg tatattcgat tccgtctttc
      481 ccaacggtat cgttcatgct gtaacccttc tttttcaacg ccgcatactg ctcggcgtaa
      541 atcgggccta ctgtcccaat cacatgaggg gcaatcgttc catccggata gacccgggtg
      601 ggaacctcac ggatttcaac cccgggcagg ctggtgctgc gctctttgat ctttgttacc
      661 gtatcaatgc tgacactgga agcaaactcg taagggttgg aataggtaaa gtcccgaatt
      721 tccatctcat agcgaatccc agcaagcgtg cgctgatctg cggaggaatt gccctcgaga
      781 tgataccgtg agacaagctt cttcataata tcagacgccc cgaccttgtc gctcacattc
      841 agcagcgaac ggagcttttt aagatccgac gtgttttttg cattaaagca gtagggagcc
      901 gttttactga tcggcagaga atcattccag ctctcgcctt tctgactcaa aattttacaa
      961 atactgttta tcacgttgtt ctgtttgacc ggctgtttgg tcgaaggaaa aaacgccttt
     1021 tgcagataga tggaatagcc cactttattg gatgccaaga cacgcccgta gcggtcgaga
     1081 atttcccctc tgggcgcttc aatttccgaa gactgaatgg tgaccttctg cgactgctcg
     1141 cggtaatccg ccccttcgac aatctggtag cgggcaagcc taacggaaaa accggtgagt
     1201 atgaaaatca ccaaaagggc cattgcaaca attctccgtc ctgatagttt catacggctt
     1261 cctcctttcg caattacatt ttttgtttat gccttccttg ttaagctgcg gtaagtttcc
     1321 ggttaatccg tcttaccccg taataaatgg ggatggtgaa cagcgccgta taaaacacgg
     1381 tcggcagaat tacatgaacc aggaagaaca ggaattcccc tcgcccggtc atataataaa
     1441 agaaaaacca ggatagcaat tcaaaaccaa acgagatccc cagggtgaga aaaatatcgg
     1501 taaacaacga acgggaaaac aaatatttga tcatcagccc caccattata cccgcgatga
     1561 gcaggaagaa cgcgttgtag ccgaatacct gaccggtctg gacatcccaa atcagacccg
     1621 cagcaatccc gtatgccgcc ccggcgtttt ccccctcaaa cattgagagc gtgattaccg
     1681 ccggaatcgc aagcaaaggg ccgttctgca ggattcgggg gaactcggga tttgtctgaa
     1741 tcaggcagac aaaaaaaatc acgatcgcgt aaaccagcca tttgacccag ttgtaattcc
     1801 gcatttactt ccccccgctc tgactggagg aacttttggt cgcgtccccg gccttaccct
     1861 gaaaactggt gatgacaaag acattcttca ctgttgacgg atcaaccatc ggtttaacca
     1921 ccgcataaag cgacagcccg ctggtctctg tccgaatctc ctcaacaata ccgatcttca
     1981 gttttttagg gaagatcccg ccgatgccgg atgttataac aatgtccccc tgggcggccg
     2041 tgctttcctt ggccagatag ctgaccttca tcctgccctt tgcgccgagt tccttgtcgc
     2101 cctgggtaat gcagggatca ttggtttgac tgaccagcgc tcccaccgac acggacgggt
     2161 ccagaatcgt tgtaacgatc gaggaggtcg acattacctg cgttactttt cctacgagtc
     2221 cgtcggatgt gataaccgga tcattaaccc cgattccgtt aagactgccc ttatcgattg
     2281 taaaaacaga aaaccactgg cccggatcgc gggcgatgac catagcgggt tcccatttga
     2341 agtcctggtt gtcatcccgg atacccaaat attccttata ttgactattt tcgtttttca
     2401 tctggttgta atcaaccagc ttggaattta aactgctgac ctcttttttc agctgctcgt
     2461 tttccttttt caccgaaccg taggaagcaa ccgttgatac agccccggaa aaagcaccgg
     2521 aaacctgagc cgaaaccttt tgcagcggag tcagaacaaa gcccatcaca ttggcgggga
     2581 tcgaggcaaa tccgcccgac ataccggtgc ggagcataag cccaaagaga atcagggcaa
     2641 tcaccgcgaa tatcttgaac catacgctcc taaaaaaatc ccgcactttt atccccatcc
     2701 caacatcatc attacggccg gctataaaag cccaaccctt actatacata aaaattatta
     2761 attggctgtt aagaaacccg tcttttcccg aaaagagaaa aaggggggac atgatacccc
     2821 gtcggaagac gggaataaca agcccccctt ttatcaatta cggattattt atgtcttgca
     2881 ttaaaaagaa cattggcaag agagccgttc atgtcttcca acattttacc cgtgccgtcc
     2941 actacgcagt caagcggacg ttctgcaata ttgaccggaa tgccggtttc ctttgcaatc
     3001 agttcatcca gccctctaag cagagcacct ccgcccgtaa gcataatgcc gcggtcaata
     3061 atatccgccg acaattcggg gggcgtacgc tcaagtgtgc tcttaatcgc gtcgacaacc
     3121 atcgaaaccg gatccgccag agcttcccgg acttcatcgg atgtaatcgt aatattgctg
     3181 ggcaagccgt caagcaaatt tctgccctta atttcgaccg aaccttcttc cggataaggg
     3241 aacgcggaac caatatttat tttgatccct tccgccgttc tttcaccaat cagcaggttg
     3301 tattttttct tgacaaagtt gataatcgca tcgtcaaact cgtcgcccgc aactctgacc
     3361 gagcgggaag tcacgattcc gctcagggag ataacggcaa cctcggccgt tcctccgccg
     3421 atatcaacca ccatggagcc ggtcgcctcg tccaccggaa gacccgcgcc aatcgccgcc
     3481 gccatcggtt cttcaatcag gtaggtgtct ttcgcgccgg cctgataggc cgcttcatcc
     3541 accgcgcgcc gctccacttc ggttacgccc gaagggatgc agataataat tctcggtttg
     3601 gagaaggctc cgccgctaat cactttttta ataaagctct tgagcatgct ggacgtaata
     3661 tcaaaatcgg cgatcacacc gtctttgagc gggcgtatcg cgacaataga gcctggcgta
     3721 cggccgatca tctgcttcgc ctcattgccg acagccaaaa cccgatcatt tttcacattg
     3781 acagcaacaa cggacggctc gcgcagaacg atgcctttgc ctttcatata aatcagtgta
     3841 ttcgctgttc caagatcaat tcctatatcg cgggaaaaca tcagtggatt gcccctttca
     3901 gaataatatc ctttttaatc gattaaacgg attttatctg aatgcatccc gcatccggcc
     3961 gtaaaccttt gtgaaacgaa gcccgccttt tggtctggct ggcaggcgtc accgggtttt
     4021 tgggtcggtc tgatgcgaca aacagatatc agcctttttt aatatgccca tttggcaact
     4081 tactcaaata ttataatcca gttttattac ggaaacaaca ctttattggg gaattaccag
     4141 attttttaat tctgcgtaac cagttcgtca tcttttcata cgaaaacgac aagtttccaa
     4201 ccggattatg gccttaaaaa cagttgttta agcaaatttc gtatcagcgc cacagaggag
     4261 ttataccgaa attttctaaa gctctgccga gccttgcaac aggaagaccc atcacattat
     4321 aataatcccc tgtaatctct ctgacaagaa gtgaaccgaa accctgaatc ccatatgcgc
     4381 cggccttgtc cagcggttca cctgtggctg tgtagcgctt aatcagttcc tgaccgagag
     4441 ggtaaaaaca aacctgtgat ttttcagcaa agctttctct gcggccgtca gccattaacg
     4501 ccaccccgct gaacacctca tgcgtcctgc ccgacagttt ttcaagcata cctgccgcgt
     4561 cctgttcatc cttcggcttt ccgagaattt tcccgccgac gctcacgacc gtatccgctc
     4621 caagaacaac ggcatccgga tactgtgaaa agatttcacc cgctttgaga acggcaaggc
     4681 gagtaaccgt ttcctccggc ggaagcggtt ccggaagatt ctcatccacc cttgagggga
     4741 tcacttcgaa ttcatagccg gcatttcgca ggatttcctc cctgcgcggg gaggaggacg
     4801 ccagaatcag cttcattcaa tcacacccgt ta
//
LOCUS       DATALY010000178         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_213584,
            whole genome shotgun sequence.
ACCESSION   DATALY010000178 DATALY010000000
VERSION     DATALY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213584"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1552
                     /locus_tag="VHR42_05190"
     CDS             203..1552
                     /locus_tag="VHR42_05190"
                     /inference="COORDINATES: protein motif:HMM:NF025756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheC/YheD family protein"
                     /protein_id="HEX3026604.1"
                     /translation="MWAKIKIIHSEKDAVYLPEPFMTPGRPFAEVRFGGIQNMAEVIF
                     THARSPEGKTFSDPAVIGLSEPLAKTLMIPLCCVYRVVLRPQSIAFGPVIGLLLGIHT
                     NRYTPDHMEKYSDRFGIYPEIGGLICAFSPKSVDWHTHSACGLIFNPEENRWEYGCFP
                     LPDVIYRRDFHMGSLFIKQLSEFTNGRLFNSYRFTKFELYDYIRNNRSLRQYIPPTEL
                     CIDFRQAFEFIRRHPTVILKPVDLSRGRGICVIEQDGEGYRVSDYRERDPHTILIADK
                     NELEQFFRKNPGFFDQYLIQQYLRLAHIGDSLFDIRVVMQKRQDRSWGCTGIECRVSG
                     PGYLLTNISRGGSALTLSEALRRAFGAEDEACKTLPPKIDALCREFCLYMDTTGEHFA
                     EFGMDIAVDTDKNIWLIEANVFPSFKGFKRTDKATYLAIRHQPLRYALSLTEFNEKA"
     gene            1569..>2179
                     /locus_tag="VHR42_05195"
     CDS             1569..>2179
                     /locus_tag="VHR42_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026605.1"
                     /translation="MRNTVFITFHHEKKAELLVNRQFADAVHLALLGFLTVGFGCLKK
                     DMFYAVSEDLPDNGFSLALSAAKDLHLPSDLPFELTYENGFLNIGPCIGLLLSREDNK
                     FKKSRLEKMLSYTHDYPAIHGAIIVFALNQMDMAGKTVTGFCFNPSVQKFERGIYPLP
                     SAVYRTVGLSKAQKEEFRTALNGRYFNSDYFSKWTMYEWLKQN"
BASE COUNT          530 a          549 c          569 g          531 t
ORIGIN      
        1 cggccagccg tattttatcg ggttcggagg ctgggacggg ctgctgcccg gaaggcctgg
       61 caaccgtgcc ggagcggctc tttgcgccga cattatggaa tatgccgccg cttttttcag
      121 cagcgaagcg gaaggatagg cgtcattccg gcttattggg tcagataaac cacaaataaa
      181 tataaatcaa ggtaatacga ttatgtgggc taagattaag attatccact cggagaagga
      241 cgccgtttat ttgccggagc cattcatgac tccgggaagg ccttttgcgg aggtccggtt
      301 cggcggaata caaaatatgg cggaggttat cttcacccat gcaagaagtc ccgaggggaa
      361 gacattcagc gacccggcag tgatcggctt gtcggagcca ttggcgaaga cgctgatgat
      421 cccgttgtgc tgcgtttacc gggttgtcct ccgcccgcag tcgattgcgt tcgggccggt
      481 gatcggcttg ctgcttggca tccataccaa ccggtataca cccgaccata tggagaaata
      541 ttccgaccgt tttggaatct accccgagat aggcggtctg atttgcgctt tttcaccaaa
      601 atcggtggat tggcacactc attccgcctg cggcctgatt ttcaaccccg aagaaaaccg
      661 gtgggaatac ggttgttttc cgcttccaga cgtaatttac cgccgcgatt tccacatggg
      721 ttcgttattt ataaaacagc tgtctgaatt caccaacggc cggctgttca attcctatcg
      781 gtttacaaaa ttcgagctgt acgactatat ccgtaataac cggtcactgc gccaatatat
      841 cccgccgaca gagttatgta tcgattttcg tcaggcgttt gaatttatcc ggcggcaccc
      901 gaccgtcatc ctgaagcctg tcgatttatc gcgaggcaga gggatttgcg tgattgagca
      961 ggacggggag ggttaccgtg tcagcgacta ccgcgaaaga gaccctcaca caattctaat
     1021 tgcagataaa aacgagcttg agcaattctt ccgcaaaaac ccgggctttt ttgaccagta
     1081 tctgatccag caatacctgc ggcttgctca catcggcgat tcgctttttg atatccgcgt
     1141 ggtcatgcaa aaacggcagg atcgttcctg gggctgcacg ggaattgaat gcagggtatc
     1201 cgggcccgga tatctgctga cgaatatttc gcgcggcggc tcggctttga ctcttagcga
     1261 agcgcttcgc cgggctttcg gcgcggagga tgaagcctgc aaaacgctcc cgccaaaaat
     1321 cgacgcgctt tgccgggaat tctgcctgta tatggacacg acgggcgaac actttgcgga
     1381 attcggcatg gacatcgccg tggataccga caaaaatatc tggctgatcg aggcaaacgt
     1441 ctttcccagc tttaaaggct tcaagcgaac cgacaaggca acttaccttg ccatccgcca
     1501 tcagccgctg aggtacgcac tgtcgctgac ggaattcaac gaaaaagcat aaacggaggg
     1561 atttttgctt gcgcaataca gtttttatca ccttccatca cgaaaaaaaa gcggagctgc
     1621 tggtcaaccg gcaatttgcg gatgctgttc atctcgcgct gttgggcttt ttgaccgttg
     1681 gtttcggatg cctgaaaaaa gatatgttct atgcggtcag cgaagatctc cccgacaatg
     1741 gtttctcact tgcattgtca gcggcaaagg atctgcacct tccatccgac cttccctttg
     1801 agctcaccta tgaaaacggt tttttgaata tcgggccctg tattgggctg ctgttgagcc
     1861 gggaggataa taaattcaaa aagagccggc ttgaaaaaat gctctcctac acgcatgact
     1921 atcctgcaat tcacggagca atcattgttt tcgcattaaa ccaaatggat atggccggga
     1981 aaaccgtcac aggcttttgc tttaacccaa gcgtacagaa gtttgaacgc ggcatatacc
     2041 ccctgccgtc agcggtatat cggacggtag ggcttagcaa ggctcagaaa gaagaattcc
     2101 gcacggcgct gaacgggcga tattttaaca gcgactattt cagcaaatgg acgatgtacg
     2161 agtggctgaa gcaaaataa
//
LOCUS       DATALY010000179         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_384530,
            whole genome shotgun sequence.
ACCESSION   DATALY010000179 DATALY010000000
VERSION     DATALY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384530"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VHR42_05200"
     CDS             <1..204
                     /locus_tag="VHR42_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026606.1"
                     /translation="LKIGRMTRDIGNIVGVISDIAEDGRITAEERPMFDHSMEQLIAL
                     KKSIEELMLVSGKNTTFEERGSE"
     gene            582..1022
                     /locus_tag="VHR42_05205"
     CDS             582..1022
                     /locus_tag="VHR42_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3026607.1"
                     /translation="MYSITIMMGRLCADPELRHTPNDVAVCTFRIAVDRPYKVKDKTV
                     ADFFDVVAWRGQGEFVSKYFRKGKPIVVQGHMENRSYTDKDGIKRYITELIAERVTFA
                     GDSSGNGSKSLPDPPPEHGDAAPAYASGNTGDFEEVPSDDDLPF"
     gene            1038..1274
                     /locus_tag="VHR42_05210"
     CDS             1038..1274
                     /locus_tag="VHR42_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026608.1"
                     /translation="MNLAAVDCDEKANERIPRLLENINVLIVQNGNGGAVRFYDAKQL
                     TRRWLSMGQQQFALLYGFDWKPPELLQCLVRLHM"
     gene            1288..>3042
                     /locus_tag="VHR42_05215"
     CDS             1288..>3042
                     /locus_tag="VHR42_05215"
                     /inference="COORDINATES: protein motif:HMM:NF022744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEX3026609.1"
                     /translation="MAVERNVLIKQLRMYGNVEDMTDWTDEQLCNMVKGYVKKDAARQ
                     YADVCPYFKGVSPYAYGARQWEIGCSLQKGCDISPYELKNWACTRARECFQPIDADFK
                     GFEHCRFFSEEKKKGDQMKQKYICKCGKTFEKESTADTTGYRLGADFGSGHECYGCHF
                     VVPVTPEGNKITDYECRASKQINYQTTADLPRSRDGFHVGRIKTLDMNFARQIWEFAK
                     KLDGQDEYREDLDLRAASFGADGRFELTLYFQKTKAGVASSLIVSDQFFDGGTSRPDM
                     TEEQEKELVLKQIQISKEKANAAPATLSSAPDQTVSNDVQNLRSIDQITLEINFYKAQ
                     TAQNIIEIGKRLIEAKQQLQHGEWLPWLRDKVEFTERSAQNFMKVAQEYSKTQPVSYL
                     PYTKLLALLQVPEAEREEFIQATHLVDGQEKAVSDMSKRELEQAIKERNEAQQNERLM
                     RERCDKAEKVAVEAREAKKKEYDNYMNALVKAEKAQETLNTLRGSRQLDLQRITELEK
                     QIKELESRPVDVAVQEPSEAEKQQLRNEGYNNAKELYDMRLQQLRNELEQAKTEARSA
                     AGFDRDEIVCAAASFRDAL"
BASE COUNT          853 a          648 c          880 g          661 t
ORIGIN      
        1 ttgaagatcg gacggatgac cagggacatc gggaatatcg tcggcgttat ttccgatatt
       61 gcggaggacg gccgaattac cgccgaagag cgccccatgt ttgatcacag catggagcag
      121 ctgatcgctt taaaaaaatc aattgaagag ctgatgctcg tttctggaaa aaacaccact
      181 tttgaagaaa gagggagtga atgactatga gtgtaaaaac attgcagggc tatatggcac
      241 tttgccggtt ctacggatgg acgccaacct gggcggggtt gaaggcgttt aacgcgggtg
      301 tccgcaaacg cgtattaggg ggtgcgggaa attgatgttt tcgccgccgc cctgtaagga
      361 ttgcacgggc cgttacatag gctgccacaa tgacaatgcc tgccctgcct ggactcaatg
      421 gcgggcagaa gaaaagcaga agaacgatat ggttcaaaac gcccgggata cggaaaagct
      481 gttccaggaa atccgttgcg caaaagatgc gcggatctgc cgccaaaagc atagtgctcg
      541 tacgcgcgga taccgggctt attaagaaag ggggctggaa aatgtactca ataacgatta
      601 tgatgggccg cctgtgcgcg gatccggagc tgcgccacac gccgaacgac gttgcggtct
      661 gcacgttccg gattgcggtg gacaggcctt ataaagtaaa agacaaaacg gtcgcggatt
      721 tctttgatgt ggttgcctgg cgcgggcagg gcgaatttgt cagcaagtat ttcagaaagg
      781 gcaagccgat cgttgttcag ggccacatgg aaaaccggtc ctataccgat aaggacggaa
      841 taaaaaggta tattacggaa cttatcgctg agcgcgtgac gtttgccgga gacagctccg
      901 gaaacggaag caaatcgctg cctgatccgc cgccggagca tggtgatgct gctcctgcgt
      961 acgcaagtgg gaataccgga gattttgaag aagttccttc ggatgatgac ctgccgtttt
     1021 agattggagt gatagtcgtg aatctcgcag ctgtggattg tgacgaaaag gcgaatgaaa
     1081 gaataccgag acttctcgaa aacattaacg ttttgattgt ccagaacggc aacggcggcg
     1141 cggtcagatt ttacgatgca aagcaactga cgcggcgctg gctgtctatg gggcagcagc
     1201 aatttgccct gctgtacgga tttgactgga agccccctga gctgctgcag tgcttagtcc
     1261 ggctgcatat gtaaggatgt gatggttatg gcggttgagc ggaacgtttt gataaaacag
     1321 ctccgaatgt acggcaatgt ggaggatatg actgattgga cggatgaaca gctgtgcaat
     1381 atggttaagg ggtacgtcaa aaaggacgca gcgcggcaat atgccgatgt atgcccttat
     1441 ttcaagggag tatcgccgta cgcatacgga gccaggcagt gggaaatcgg atgttctctg
     1501 caaaaaggct gtgacatttc accgtatgag ctgaagaact gggcctgcac tcgggcaaga
     1561 gaatgtttcc agccaataga tgccgatttt aagggatttg agcattgccg attttttagc
     1621 gaagaaaaga aaaagggtga tcaaatgaag cagaaataca tatgcaaatg cgggaaaacg
     1681 tttgaaaaag aatctactgc tgatacaacc ggctaccgct tgggcgcgga ctttgggtcc
     1741 gggcatgagt gctacggatg ccattttgta gtcccggtca ctccggaagg aaataagatc
     1801 acggattatg agtgccgagc aagcaagcag atcaattatc aaacgactgc agatttgcct
     1861 cggtctcgcg atggttttca tgttggacgg attaaaacgc ttgatatgaa ctttgcccgg
     1921 cagatttggg aatttgcaaa aaaattggac ggtcaggatg aataccggga agatttggat
     1981 ttacgcgcag cttcgttcgg cgcggacggt cgctttgaat taaccctata ttttcagaaa
     2041 acaaaggctg gcgttgcatc cagtcttatc gtatcagatc agttttttga tggtggtaca
     2101 agccgcccag atatgactga ggaacaggaa aaagagctgg tgttaaagca gattcagatt
     2161 tcaaaggaaa aggcaaatgc ggctccggct acattgtcgt cggctcctga tcagacggtg
     2221 tccaatgacg tccagaattt gcgttcgatt gatcagatta cgttggaaat caatttctat
     2281 aaggcccaaa cggcacagaa tatcatcgaa atcgggaaac gcctgattga ggcaaagcag
     2341 cagctgcagc atggggaatg gcttccctgg ctgcgggata aggtggaatt tacagagcgg
     2401 tcagcgcaga attttatgaa agtcgcgcag gagtattcaa aaacgcaacc ggtttcgtat
     2461 ttgccctata caaagttgct tgccctcctg caggtgccgg aagctgaacg ggaagaattt
     2521 attcaagcca cacacctggt tgacgggcag gaaaaggctg tttctgatat gtcaaaacgc
     2581 gagctggaac aggccataaa ggagcgcaat gaagcacagc aaaacgaaag gctcatgcgt
     2641 gaacggtgcg ataaagcaga aaaggtcgcg gttgaagcca gagaggcaaa gaaaaaggaa
     2701 tacgataact acatgaatgc gctggttaaa gctgagaaag cacaagaaac gctgaacact
     2761 cttcggggca gccgccagct tgatctgcag cgcatcacag aactggagaa acagattaag
     2821 gaactggaat cccgcccggt agatgttgcc gttcaggagc cctctgaggc tgaaaaacag
     2881 caactgagga acgagggtta taacaatgcc aaagaattgt atgatatgcg ccttcagcag
     2941 ctccggaacg agctggaaca ggcaaaaaca gaagcgcgca gcgctgccgg ctttgaccgc
     3001 gacgaaattg tgtgcgctgc cgcttcattc cgggatgccc tc
//
LOCUS       DATALY010000180         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_557182,
            whole genome shotgun sequence.
ACCESSION   DATALY010000180 DATALY010000000
VERSION     DATALY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557182"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="trmFO"
                     /locus_tag="VHR42_05220"
     CDS             complement(<1..1088)
                     /gene="trmFO"
                     /locus_tag="VHR42_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX3026610.1"
                     /translation="MKISVVGAGLAGCECAWQAANLGLDVELFEMKPEKFTPAHHSPD
                     FAELVCSNSLRADRVENAVGLLKEEMRRLDSVIIRSADETRVPAGGALAVDREAFSRL
                     ITEKIRSHPRITVIAREVDTIPDDGYCVIATGPLTSESLSKEIQKYCGEEYLHFFDAA
                     APIVEFDSINMDKAYFAARYGRGGADYINCPMDQAEYEAFYRALLDAETAELKEFDQK
                     ELKVFEGCMPVEVMAQRGRDTLRFGPLKPVGLADPKTGRTPYAVVQLRRDNAAGSLYT
                     LVGFQTHLKFPEQKRVFSMIPGLEEARFVRYGVMHRNTFLDSPRLLDCCYRLKSEPSV
                     FFAGQMTGVEGYVESASSGLVAGVNCART"
     gene            complement(1085..>1672)
                     /locus_tag="VHR42_05225"
     CDS             complement(1085..>1672)
                     /locus_tag="VHR42_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEX3026611.1"
                     /translation="QLKPTPLGEVTIGLMKEHFANVVDVEFTANMENDLDRIETGERD
                     WKDTLSQFYSGFNQTLQKAEEEMGDVHVKVPDIVTDVKCELCGRNMVIKTGRFGKFLA
                     CPGYPECKNTKAIAQETGAFCPVCGGKVLLKKSKSGKKYFGCEKNPKCPFMTWDEPLK
                     ENCPRCGSSLFKKRGGVIYCAKEGCGYEKERSPKE"
BASE COUNT          377 a          504 c          396 g          395 t
ORIGIN      
        1 gtccgggcgc agttcactcc ggccacaagc cccgacgatg cggattccac atagccctcc
       61 actccggtca tctgacccgc aaagaaaacg gacggctctg atttgagcct gtaacagcaa
      121 tccagcagtc ttggcgagtc aagaaacgtg ttgcggtgca tgacgccgta acggacaaac
      181 cgcgcttcct ccagcccggg tatcatggag aaaacccgct tttgttcggg aaatttcaaa
      241 tgggtctgaa acccaaccag agtataaagg ctccccgcgg cattatctct gcgcagctgc
      301 accaccgcat aaggggtccg gccggttttc gggtcggcca gcccaaccgg tttaaggggg
      361 ccgaaacgga gcgtatccct gccgcgctga gccattactt cgaccggcat acaaccctca
      421 aacaccttga gctctttttg gtcaaattct ttcagctcgg cggtttccgc gtcaagaagc
      481 gcccgataga aagcctcgta ctccgcctga tccatcgggc agttgatata atcggcgcct
      541 ccccggccat aacgcgccgc aaagtaagct ttatccatgt tgatgctgtc aaattctaca
      601 atcggcgccg cagcgtcaaa aaaatgaaga tattcctctc cgcagtactt ttggatctcc
      661 tttgaaaggc tttccgatgt cagcggaccc gtggcaatca cacaatatcc gtcgtcggga
      721 atcgtatcaa cctcacgcgc tataaccgtg atccgtgggt gcgagcgaat tttctccgta
      781 atcagccgtg aaaacgcttc ccggtcaacg gcaagcgcgc cgcctgccgg aacccgggtt
      841 tcatcggcgg accggatgat caccgaatca agcctgcgca tctcttcctt gagcaggccc
      901 acggcattct ccactctgtc ggcacgcaga gaattggagc agacaagctc ggcaaaatca
      961 ggcgaatgat gagccggagt aaacttctcc ggcttcattt caaaaagttc aacatccaag
     1021 cccagattag cagcctgcca ggcacactcg cagcccgcca gcccggctcc gacaacggat
     1081 attttcactc tttcgggctc ctttccttct cataaccgca gccctctttg gcgcaataaa
     1141 tcacgccgcc tctttttttg aacaggctcg aaccgcagcg cggacagttt tccttcaacg
     1201 gctcgtccca tgtcataaag ggacacttgg gatttttctc acagccaaag tatttttttc
     1261 cgcttttcga cttttttaac aaaacttttc cgccgcaaac agggcagaat gctcctgttt
     1321 cctgcgcgat cgctttggtg tttttgcact cggggtatcc cgggcaggcg aggaatttac
     1381 cgaaccgccc tgttttgatc accatattgc ggccgcaaag ctcacatttg acatccgtga
     1441 cgatatccgg aactttgaca tgcacgtcgc ccatttcttc ctccgccttt tggagcgtct
     1501 ggttgaaacc ggaataaaat tgggaaagag tatccttcca gtcgcgttca ccggtctcga
     1561 tccggtcaag gtcgttttcc atattggcgg tgaattccac atccaccaca ttggcaaaat
     1621 gttctttcat cagcccgatc gtcacttcgc caagaggagt gggctttagc tg
//
LOCUS       DATALY010000181         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_301225,
            whole genome shotgun sequence.
ACCESSION   DATALY010000181 DATALY010000000
VERSION     DATALY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301225"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VHR42_05230"
     CDS             complement(<1..1506)
                     /locus_tag="VHR42_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026612.1"
                     /translation="MALIINRGVAGHDTALVLKLGGLMILMATFGYCSAFVCQLFAAR
                     ASQGFGTDLRNDVFYHILRYSYVQIDDFGAPTLTNRITNDINQLQQWVAMMIRLVPRA
                     PFICIGAIILSFFLDPKLALILLASMPVLAAIIFIITKFASPLYREYQKRLDRIARVL
                     RENLSGVRVIRAFSKAGEETTRFKSANDKLMDTGLTIGKISSFFNPLTSLVVNFVIVL
                     ILWFGGIRISAGHLSQGQIIAFINYVNQILYALLVLSNLIILITKAMASAARVNEVLD
                     TKPGMTGGGSKAEIFSQAPAVEFRNVSFGYNETGEKALEHVSLKIRRGETLGIIGGTG
                     SGKTTFVNLIARFYDADSGTVLIDGTDVKKYFLPELHQKIGVVPQKALLFSGTVAENI
                     RWGKADADEEAVAQAARTAQADEFIRELPQGYDTPISRGGTNLSGGQRQRLTIARAVV
                     ANPEILILDDASSALDFLTDARLRAAIREKSRDMTVILVSQRAGVIKNADRI"
     gene            1663..>2906
                     /locus_tag="VHR42_05235"
     CDS             1663..>2906
                     /locus_tag="VHR42_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026613.1"
                     /translation="MKKNTFVRLFSYVKGYHFLLAFSAVSAFLSVLLTLIAPLVIGRS
                     IDQMKGIHHVDFTNISKTLFLLILIYLGSNLFLWLLTFLTNKISYRTVNKLRKLLFEK
                     LNTLPLLFFDRNPHGDTISRFVNDTDIISDGMLQGFMALLSGILTIVGAIAFMIYINP
                     GMTLVVVLSAPASYFVTRFIAKRSQQLFGEQAKELGLLNGYAEEMIQGQKVVKAFSYE
                     DQAYDTFRKINSKLYNAGVKSQFISSLSNPSTRIVNNITYALIGIIGGVAAISGEITV
                     GDISSFLIYAIIFSKPFNDITNVFTQLQSAVASAQRVFHILDLAPETLDAEDAIELRD
                     CNGNVAFQNVRFSYNPGQRLVENFNLEVPSGSSIAIVGHTGAGKTTLVNLLMRFYDID
                     GGSITIDGTDIRKIRRDSLRSN"
BASE COUNT          720 a          723 c          721 g          742 t
ORIGIN      
        1 tatcctgtct gcgtttttga tgacgcccgc acgctgggaa acaagaatca cggtcatgtc
       61 gcggcttttt tcccggattg ccgcacgcaa acgtgcgtcg gtgagaaaat ccagcgcgct
      121 ggaagcgtcg tcaagaatta agatttccgg atttgccacg acggcccggg caattgtcag
      181 cctctgtcgt tgtccgccgg aaaggttggt gccgccgcga cttatcggcg tatcataccc
      241 ctggggcagt tctctgataa actcatccgc ctgcgccgtg cgggccgctt gggcgacagc
      301 ttcttcgtca gcgtccgcct ttccccatcg gatattttca gcgactgttc ctgagaaaag
      361 cagtgccttt tgcggtacga cccctatttt ctgatgaagc tccggaagga agtatttttt
      421 cacatcggtt ccatcgatca gtacagttcc ggagtccgca tcataaaaac gggcaatgag
      481 gttaacgaac gtggtctttc cggagcctgt gccgccgatg attccgagcg tctcgcctcg
      541 ccggattttc aggcttacgt gctccagcgc tttttcaccg gtttcattat acccaaagga
      601 gacatttctg aattcaacgg ccggcgcctg gctgaaaatc tccgctttgc ttccaccgcc
      661 tgtcattccg ggcttcgtat cgagcacttc attgacccgt gccgcggaag ccattgcctt
      721 tgtgatcagt atgatcagat tggaaagcac cagcagggca tacagaatct gattgacgta
      781 attgataaat gcaatgatct gaccttggga aaggtgcccg gcgctgatgc gtatcccgcc
      841 gaaccaaaga atcagtacaa tgacaaaatt aacgacaaga gaggtcagcg ggttgaagaa
      901 agaagaaatt tttccaatcg ttaatcccgt gtccatcagt ttatcattgg cagatttaaa
      961 acgtgttgtc tcttctccgg ctttggagaa agcacggatg acccgaacgc ccgaaaggtt
     1021 ttcccggagc acacgggcga tccggtcaag ccgtttctga tattcgcgat aaagcggaga
     1081 ggcaaatttc gtgatgataa aaataatggc tgccaaaacg ggcatggagg caagcagaat
     1141 cagtgcaagt ttcggatcaa ggaaaaagga aagaataatc gccccaatgc agataaacgg
     1201 agcgcgggga accagacgga tcatcattgc cacccactgc tgaagctgat tgatatcgtt
     1261 cgtaatccgg ttcgtcagcg ttggagcacc gaaatcatca atttgcacat aggaatagcg
     1321 caggatatga taaaatacgt cgttgcgcag atctgtccca aatccctgag aagcgcgcgc
     1381 ggcaaaaagc tgacagacaa atgcgctgca atagccgaag gttgccatca gaatcattag
     1441 cccgccgagt tttaacacaa gggcggtatc atgtcccgca acaccccggt tgatgatgag
     1501 cgccataatc gtaggcagaa gcaattcgaa aaacgcagac aggatattgg tgttggaaga
     1561 tgggaaagtc gcggggttgg gtacccatga agagcttata catagctgcg gcgtttatcg
     1621 tgagatctgc ctttcgcagc tttccactca ggaggcaggg ctgtgaaaaa gaatactttt
     1681 gtcagactct tttcctatgt taagggttat cattttctgc tggcgttctc tgcagtaagt
     1741 gcctttttga gcgtcctgct gaccttaatt gcccctcttg tgatcggccg gtcgatcgat
     1801 cagatgaagg gaatccatca tgtcgatttc actaacattt caaaaacgtt gtttcttttg
     1861 attctgatct atctcggcag caacctgttt ctttggcttt tgacctttct gacgaataaa
     1921 atttcttatc ggacggttaa caagctccgc aagctgctgt ttgagaaact gaacaccctg
     1981 ccactgcttt tttttgaccg gaacccgcat ggggacacaa ttagccggtt tgtcaatgat
     2041 acggatatca tttcggacgg catgctgcag ggtttcatgg ccttactctc cggaattctg
     2101 accatcgtcg gggcaatcgc ttttatgatc tatataaacc ccggaatgac gctggtggtt
     2161 gttctttccg ctcccgcctc ttatttcgtt acgcgtttta ttgcgaagcg gtcacagcag
     2221 cttttcgggg aacaggcaaa ggagctcggc cttctgaacg gttatgcaga agaaatgata
     2281 cagggccaga aagtggtgaa agccttcagc tatgaagatc aggcatatga tactttccgg
     2341 aaaatcaaca gtaaactcta taacgcgggt gtaaaatcgc agttcatttc ctcgctgtcc
     2401 aatccctcca cgcgtatcgt aaataatata acctatgcct taatcggcat catcggcggc
     2461 gttgccgcga tctccggaga aatcacagtc ggggatattt ccagcttcct gatttacgcc
     2521 attatctttt caaaaccctt taacgatatc accaatgtct ttacgcagct gcaatcggcg
     2581 gtggcctccg cccagcgtgt attccatatt cttgaccttg cgccggaaac tttagacgcg
     2641 gaggatgcca ttgagctaag agactgcaac ggaaatgtcg cgtttcaaaa cgtccggttt
     2701 tcctataacc ccggtcaaag gctggttgag aatttcaatc ttgaagtccc gtccggaagc
     2761 agtatcgcca tcgttggcca tacgggcgcg ggaaaaacaa cccttgtaaa ccttctcatg
     2821 cgtttctatg acatcgacgg tgggagcatc acgattgacg gaaccgatat ccggaagatc
     2881 aggcgagata gccttcgaag caattt
//
LOCUS       DATALY010000182         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_815522,
            whole genome shotgun sequence.
ACCESSION   DATALY010000182 DATALY010000000
VERSION     DATALY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815522"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..503)
                     /locus_tag="VHR42_05240"
     CDS             complement(45..503)
                     /locus_tag="VHR42_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX3026614.1"
                     /translation="MENPTAELIPTQRIAYIRHTGAYGPGNRKTMESLKQWAREKGLF
                     QSSAVIYAIAWDNPLTTAPEKCRYDVCIKLDDNFAVDGNVSEGTFEQGWYAVFTIVHT
                     AEAIQQAWQSFIQKTQESGYRIDETRVVVERYRQELVDNHFCELCVPILK"
     gene            complement(611..1042)
                     /locus_tag="VHR42_05245"
     CDS             complement(611..1042)
                     /locus_tag="VHR42_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX3026615.1"
                     /translation="MLYLAYGSNLNLEQMVARCPTAKIAGAATLEDYRLLFRGGRSNA
                     VATVEPQEGDSVPVLLWEITLADEAALDRYEGWPYLYRKEILTVLLNGKPTGAMVYIM
                     NEGRPLGKPSCYYYNIILQGYRSAGFDVEILRKATASSTEI"
     gene            complement(1174..>2005)
                     /locus_tag="VHR42_05250"
     CDS             complement(1174..>2005)
                     /locus_tag="VHR42_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HEX3026616.1"
                     /translation="ADCLGGMIIDGRDYYDTKKITAPDGRVWKIMSDGSIRTEKKERN
                     RVIAATGEYSVELVSPILTYREDIDTLQELIRRIRHAGGFANSSCGIHIHLDSANHTP
                     RSIRNFVNIIASKNDLFYKALQIDPARMRFCKKMDDTLVETMNRKKPKNMRELEEIWY
                     AGYNSSRSTHYHSSRYHFLNLHSFFNGNHTVELRGFNSELHAGKIRSYIVLVLALNNQ
                     ALTQKCASAKKPQVENEKFAMRTYLNRIGLIGEEFKNCREHLCSQLDGSAAWRFGSAA
                     "
BASE COUNT          414 a          541 c          482 g          568 t
ORIGIN      
        1 aggaaagagc ctccgaagaa gctcctgtct gttacctgtt ctacttattt tagaatcgga
       61 acacacaatt cgcaaaaatg gttatctaca agttcctgac gatatcgctc cacaacaact
      121 ctggtttcgt caattcggta gcctgactcc tgtgttttct gaataaaaga ctgccatgcc
      181 tgctgtatgg cttccgccgt atggacaatt gtaaaaacag cataccagcc ttgttcaaaa
      241 gtgccttcgc tgacgtttcc gtctaccgcg aagttatcgt ccagttttat acatacatca
      301 tatcggcatt tttccggtgc agtcgtcaga gggttatccc aggcaatggc atagattacc
      361 gctgagcttt gaaacagccc tttctcccgc gcccactgtt ttaagctctc catagttttt
      421 cgattcccag gaccgtaagc accggtatgt cgtatatacg caattcgttg tgtgggaatt
      481 aattcagccg ttggattttc catctttgaa tactcctttc ccgtggagta ttcagtatat
      541 atcctattaa aatgaaaaac aacggcattt aaaaactttt tctcttgctt ctctcgtcag
      601 gatagatcgg tcatatttct gtggaactgg cggtggcttt tcgaagaatt tccacgtcaa
      661 atcccgccga gcggtacccc tgcaaaatga tgttgtaata gtaacagctc ggttttccaa
      721 gtggtcggcc ctcgttcatg atgtaaacca tcgcgccggt tggctttcca ttcagcaaga
      781 ccgtcaagat ttctttccga taaaggtacg gccacccttc gtagcggtcc aatgccgctt
      841 cgtcggctag agtgatttcc cacagcagga ctggaacact gtcgccttcc tgcggttcaa
      901 cggtggcgac ggcattagag cgtccgccgc gaaacagaag ccggtagtct tccagcgttg
      961 ccgctcctgc aatcttggcg gttgggcacc gcgcgaccat ttgttccaga tttagattcg
     1021 agccataagc taagtaaagc atcttttatt cccctacgcg ttttgttcgg gccgtgcggc
     1081 ccagtgtcgc tttttctttt tcttgcggaa caccgttgcc agcaaacctt ggcggggcac
     1141 acggtcccac cgttagcttc accggcggtt ccttcaggca gcggaaccga accgccatgc
     1201 cgccgaaccg tctagctggc tgcaaaggtg ttcccggcag tttttgaatt cttcgccaat
     1261 cagaccgatt cggttcaggt atgtacgcat cgcgaacttt tcattttcga cctgcggctt
     1321 ttttgccgat gcgcatttct gcgtcagcgc ctgattgttc agggccagaa ccaacacgat
     1381 gtagctcctg attttcccgg cgtgaagctc gctgttgaac cctctcagct ccaccgtgtg
     1441 gttgccgttg aaaaagctgt gcaggttcag aaaatggtac cggctgctgt ggtaatgggt
     1501 gctgcggctg gaattgtaac ccgcgtacca aatctcttcc agctcccgca tgttcttcgg
     1561 ttttttccgg ttcatggttt ccaccagcgt gtcgtccatc tttttgcaaa accgcatccg
     1621 tgccgggtct atctgcaggg ctttatagaa aaggtcgttc ttgctggcga tgatgttcac
     1681 aaagtttcgg atgctgcgcg gagtgtgatt tgcgctgtcg aggtgaatat ggattccgca
     1741 ggagctgttc gcaaacccgc ctgcatggcg aatccggcgg atcagctcct gcagggtatc
     1801 gatgtcctcg cggtaggtaa gaatcggact gaccagctcg acgctgtatt cgcccgttgc
     1861 ggcaatgacc cggttccgtt cctttttctc ggtgcggata ctgccgtcgc tcatgatttt
     1921 ccaaactctg ccgtccggcg cggtaatctt tttcgtatcg tagtagtccc gaccgtcaat
     1981 aatcattccg cctaagcaat ccgca
//
LOCUS       DATALY010000183        16259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_557532,
            whole genome shotgun sequence.
ACCESSION   DATALY010000183 DATALY010000000
VERSION     DATALY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557532"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..170)
                     /locus_tag="VHR42_05255"
                     /pseudo
     CDS             complement(45..170)
                     /locus_tag="VHR42_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
     gene            complement(471..1016)
                     /locus_tag="VHR42_05260"
     CDS             complement(471..1016)
                     /locus_tag="VHR42_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HEX3026617.1"
                     /translation="MKKWVCAVCGYVYEGEEPPEKCPQCGATKDKFSEKKDGALAWAD
                     EHKIGVAKGVDPEVVEGLKANFTGECTEVGMYLAMSRQADREGYPEIAEAYKRIAFEE
                     AEHAAKFAEMLGEVVLPDTKKNLEMRVEAEFGACQGKKDLAVRAKQLNYDAIHDTVHE
                     MCKDEARHGAAFNGLLKRYFK"
     gene            1336..2553
                     /locus_tag="VHR42_05265"
     CDS             1336..2553
                     /locus_tag="VHR42_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3026618.1"
                     /translation="MPEFVYTSKDQTGKTYSGVIDADSIDSFYHIIKDRSQYCITVKE
                     SGVPQKEIVFGSKKFNLKELSVFCRQFSTMLNSGLPVIKCLDILYQQTTNKKMRSSIL
                     GVYETVQRGESLSSAMKHQKTAFPSLFIHLVEAGEASGSLDSVMNRMAEQYEKENKLQ
                     NKVTQALIYPIFLCCLSIVVVIFLLTFVLPTFLSMFTQFGGQLPVTTKILLGISHLFT
                     NYWYLILILLAGIYIGFRALMRKHNVRLGWDKLKLRLPIIGKLLLILESSRFARTLAS
                     LFNSGMPVMQSVEITANVMTNSYIRNRLFHVNEEVRRGSSISASIRKLNLFPLMLCSM
                     LNIGEESGNMDVIRLKTSAFYDEEADAALQKLVSLIEPFMIVLLAVVVGFIIVSIITP
                     IYGVYSQISKTGM"
     gene            2570..3580
                     /gene="pilM"
                     /locus_tag="VHR42_05270"
     CDS             2570..3580
                     /gene="pilM"
                     /locus_tag="VHR42_05270"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX3026619.1"
                     /translation="MLLSIDVGSVSLYAVQGSCSAGAVSIDRAGEIALPEVTIEDGMI
                     KNHASFIMTVNKLLTLCRFTARNTVFTITSSSVLSRRLELPASTPRELNMMVNNEMIQ
                     VVNDSSDFVYEYSIIGPEHASKQKTIPVWAYALPRDCVDQYYSVFNGIRLKPKALDIH
                     PNSAEKLFVNSSVNGNEIGQKSILFADIRQNDVEIHLISDGQRAFSRTSPVSSSTFEA
                     ILNNSGLVPENVSMFDSLDITSDKFRGNSILNDAAHQYINQICEELQKMVQFQLRRNS
                     TNPVAGVYIYGNMSGIRGLTEGMSSLLGIHVEKVEAVSKVKAGNLEIAKYINAIGALI
                     RL"
     gene            3589..4155
                     /locus_tag="VHR42_05275"
     CDS             3589..4155
                     /locus_tag="VHR42_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026620.1"
                     /translation="MQRDINFFSVYHSSADHGGVDRTTLIGGIVTIASVVIVLGAFLL
                     LKIGTAGLSMQSQSISQFLARPDVSAATSRVNEIYAKNNYLDKYQSTASSNIKKFYAL
                     TKFDSKLIDLISKAAPADVTASDLNFGGNIVSLTCTATDERSPANFVHTLSATGSFEQ
                     VTYDGVIKDSNSQYTFSVDCIMKGESDK"
     gene            4152..4925
                     /locus_tag="VHR42_05280"
     CDS             4152..4925
                     /locus_tag="VHR42_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026621.1"
                     /translation="MTLTSREKKLLFVLALVVILVGGFRFLVLPAYSKFNTENENFST
                     LLARQLIAKGNLAAAGTIDSGLQKKLSDVDSSASALFPSLSNDQLNIWILDLTAKNGL
                     SVSSIKFGSPYVTEISKDNIKNADSSTSGSTSAGQNQTAAGDLNYLLKKYAQGYKGSS
                     TSAVPATTSGSSSSSSSAQSGASSQQAGNSLVGEQIKISMTGSFAQANSFLDAVKNSK
                     KTVLVSSFSCSPKGSTLTIDATLLFYGAEKPDSSDKTFQ"
     gene            4996..6696
                     /locus_tag="VHR42_05285"
     CDS             4996..6696
                     /locus_tag="VHR42_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX3026622.1"
                     /translation="MNCRQGKITSNRNSSGSSLIMVLIIFAMFLMLSAALLSAAFTST
                     AMAGKQVTSQQNYLSARSANASLLKVFTDSTNSGWQALKDKIRLMTVGDTLTGEINDP
                     NLGHIRIVITKTGSTTTGMTFDIKSTTILSDGTQSAPVILHLSAPITSSVVYDASNPY
                     NSLFYYTGSVTHYMETGNATGMLIYNGPVYIAQAGGSNNNVKGNVFSTDVIYLSGSQT
                     GITYLASNKDIFLQDTYKLSSNGYYYSDNNPVIGTSAVPCPVIAKGSLSVGGHYNSND
                     YPIIYGNVTVDGNVTLTDHCRIYGNITAGGAVRIDGGCTVQGNVVAGGGITGSGAVTG
                     TKSPNTKNIPKVTIIYTPTDKLPAITPPAYSNAVAVGSGGTINTSGVLSSSSFNNSDT
                     LYVDTSAADVNLVIKNDLTLGYSDWWNNYSQKILVSGSHNLYIYLASGSDLTLRSSSQ
                     IGMRDGTTGNVSSSTSQSGWPHIFIIGNNQTLTLNDTSLLRANIFLPNGTFAGNWSNG
                     ASSNNRFMGSALIGDDTIIVSSKSQSLYFLKPTLDNTPLSGFGTQKYAVGNYTGSWSK
                     "
     gene            6693..7094
                     /locus_tag="VHR42_05290"
     CDS             6693..7094
                     /locus_tag="VHR42_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX3026623.1"
                     /translation="MKKFIRNHLRNQRGSTLVEVLVSLALFAIVAIFILSTLLTGITI
                     VSSAKSKSQNSMKTAGGLAQKTENQTVSDPQGTTVSSSSGNFTMTLTDSADGSQITVT
                     VPGSFLTGTTSGKNPGESVTYKSFSPSDSGS"
     gene            7107..7667
                     /locus_tag="VHR42_05295"
     CDS             7107..7667
                     /locus_tag="VHR42_05295"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX3026624.1"
                     /translation="MKQRLKSQKGLTLVELMISMAVSMILLGACAGLLVYGFNLYNKA
                     NDYNQAHVLGLLTQQKIESEVKYAASMKIDASQPGSLANGNYCLALTGSGRLAKYTGT
                     ASAMTSNYVYLDGVYRGYVCSITFKASTGSTLNVEVTVSKGGETITDTTTDIILNNMG
                     KNIPSGQDINATVTQGNAIEFTLPSA"
     gene            7723..8484
                     /locus_tag="VHR42_05300"
     CDS             7723..8484
                     /locus_tag="VHR42_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3026625.1"
                     /translation="MVISKSNNLNSVFQQYLDYPCHERLDAVAEAGRPLVIHFASLYG
                     SGCPFDDIYQTGMLGLVKAVKTYDGSTLFTTWASNCIIGEIRHFVRKERNYHYPDCIG
                     QLQQQADLLIETAVKTDGPPPDTGALAQKLGVSREGYREVMRAGLVSFNEIDVSKISS
                     AQAQSFHLPIEDKIALDQAIGRLSEIQKHVVHALYYRGLTQQQTAEELGINQRKVSRI
                     KTGVIRLLGEMLNEPNFRLIDSSRSFVPINKKSKK"
     gene            complement(8542..8949)
                     /locus_tag="VHR42_05305"
     CDS             complement(8542..8949)
                     /locus_tag="VHR42_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010642060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX3026626.1"
                     /translation="MIQTLIKKIGKDKKGFTLIELLVVIAILGILAAILIPTVSGYIN
                     SARDSVATADARTVYSAVSAYTAAHSDVTYTAYVDSNKPATGASETTIYKDDLNTYLG
                     THSFSIAVTIPSGSPTIVTVTESNGRTGTYTKS"
     gene            complement(9025..10086)
                     /locus_tag="VHR42_05310"
     CDS             complement(9025..10086)
                     /locus_tag="VHR42_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX3026627.1"
                     /translation="MDLINELLIKAIEKSASDLHVTVGVSPMVRINGQIQPLEGYEPF
                     RPEDTKTICMAMLGEEQKNVLEKNGQVDFSYVVPKVGRFRVNIYKQRRSFSAAIRVLV
                     QQMPTIESLGLPEILKELTLRPRGLILLTGPTGSGKSTTLAAMVDYINSNKRCHVLTI
                     EDPIEYLHKHKNSIVNQREIGDDTSSFSGALRSALREDPDVILVGEMRDLETIATAIS
                     AAETGHLVLSTLHTIGAAQTVDRMIDMFPPYQQQQIRVQLASVLQAVITQQLITNTTH
                     SNRIAALEIMTVNDAIRNMIRESKCHQITSALQTGIKQGMMPMDYHLSRLVRQGSISS
                     DEALSHCMDVDTFKRYLNI"
     gene            complement(10101..11780)
                     /locus_tag="VHR42_05315"
     CDS             complement(10101..11780)
                     /locus_tag="VHR42_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX3026628.1"
                     /translation="MPFKNVPLGQILIEANLITKQQLDQALEIQKTDGTRKLGEILVN
                     MGALGERDQMKALEDRFKIPLIDLNVEAVDPKAPALIPENIAVKYNIIPVGVEGKYLT
                     VATSDPMNFYALDDVRLVTNMEVRVVLASYSDIKAAIGKYYSRTQAEKAAKDLSREFD
                     GREIEELSEQVGERVDNAPVVRLVNSMIQQAVKLGASDIHIEPMEESTRVRIRIDGVM
                     RNQMTLAAGAHASIVTRLKIMGNMNIAERRIPQDGRVETVIDGQPVDLRLSVLPTVTG
                     EKVVIRILGGTGTVLDVDDLGLSERNRGLFEQILKNSNGIILICGPTGSGKTTTLYSM
                     LNTFNDPGVNIITIEDPVEYRMPGINQVQVNPKAGLTFASGLRSILRQDPDIIMVGEI
                     RDSETAQIAVRAAITGHLVLSTIHTNDAASTVSRLVNMDVEPYLVSSAVTGIVAQRLV
                     KRVCPKCGVDYLPGQEEMRQLGLTAPIKLRHGLGCNYCDNTGYKGRAAVHEIIVMSQK
                     IRELVESGASADEIREISTKSGTVTLQESCRELVLKGLTTTEEMMRATYNI"
     gene            complement(11892..12665)
                     /locus_tag="VHR42_05320"
     CDS             complement(11892..12665)
                     /locus_tag="VHR42_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX3026629.1"
                     /translation="MQNILILIEYFFVFTAGLALGSFLNVCIYRIPVHESIVTGRSHC
                     TSCGNAIKDYDLIPVVSFILLRGRCRSCGAKISWRYPLVEMLNAVAYVLIFTFSGATA
                     LSFVYAAAVSAYIVIAFIDFDKSIIPDSIVLYIIIIGVIALIISHNIPAYERIIGFFS
                     ISVPLLAAAILSKGGMGMGDVKYAAAAGLVLGYKLALLGLFLGSVAGALFGVVYAVKS
                     KKSLKTSIPFGPFLSLGFGLSMLFGNVILNFYFSLFRPC"
     gene            12845..13462
                     /locus_tag="VHR42_05325"
     CDS             12845..13462
                     /locus_tag="VHR42_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX3026630.1"
                     /translation="MHEFDKCIITNKNGAKISEGIVSEYNSADMRINTTESHNLRVYD
                     EVLIYVYNQLKGECKYEGTVSQATGSRITLFNVSLVRSVQKRDNTRVSKQLKYMVTHS
                     FRGDRAIKLEKPIEITVLNISAQGLYLNSTHDFAIGSRFPLVLQETIKPIKVTVEILR
                     KEKYTRSFNYGCQFADISQKDMDEIFRFVLKEQIDQRRRNLLFHP"
     gene            complement(13518..14522)
                     /gene="tsaD"
                     /locus_tag="VHR42_05330"
     CDS             complement(13518..14522)
                     /gene="tsaD"
                     /locus_tag="VHR42_05330"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX3026631.1"
                     /translation="MKILALESSCDETAAAVVEDGRKVLSNIVSSQIQTHRIYGGVVP
                     EIASRMHVEVIASITRQALEEAGCDFKDIDGVAVTYAPGLIGALLVGVNFAKGLSLTM
                     DVPLIPVHHLRSHIAANYIASPQLEPPFLCLVASGGHSHLVEVSGYTSFRVLGRTRDD
                     AAGEAFDKAARSLGFPYPGGVYIDRAAKLGDPKKVALPRPHVDGSPYDFSFSGLKTAV
                     INLVHNASQKSEQLNPNDVAASFQQAVVDILSDRLMLAAEATKSRKIVVAGGVAANSG
                     LRERLTDECSRRGFSLFMPPLSLCGDNAAMVGCQGYYEWVSGHTAGTDLNACANMEID
                     "
     gene            complement(14538..15005)
                     /gene="rimI"
                     /locus_tag="VHR42_05335"
     CDS             complement(14538..15005)
                     /gene="rimI"
                     /locus_tag="VHR42_05335"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX3026632.1"
                     /translation="MTEDRSAVEIVPMRPEHLEKLAEVERLCFSEPWTVQGLAEEIEN
                     PLAVFLVAELDGVTVGYAGMYHVLDEGYLTNVAVDPGFRRRGAARALLDWLDGYAQKK
                     SLQFITLEARVSNRPAIALYHASGYREQGIRPGFYANPREDAVIMTKFYDSTK"
     gene            complement(15002..15448)
                     /locus_tag="VHR42_05340"
     CDS             complement(15002..15448)
                     /locus_tag="VHR42_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HEX3026633.1"
                     /translation="MKKLLFVCTGNTCRSPMASALMNKMLQENGITDVVADSAGIAAA
                     EGTPASANAVAAAGEAGLDLSRHRSKLLSRELIEQADRIYVMSPSHKMAIVASYPQAR
                     DKIVDLGGIPDPYGAGIEVYRRCRDELIQRIRPVVGQIKRRNEGKV"
     gene            complement(15464..>16259)
                     /locus_tag="VHR42_05345"
     CDS             complement(15464..>16259)
                     /locus_tag="VHR42_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX3026634.1"
                     /translation="TGRISSTEPNLQNIPVRSEAGRELRRFFIPAQGCELIDADYSQI
                     ELRILAHVAGDQAMQDAFNSHADIHTITASQVFRLPPEMVTPVLRSRAKAVNFGIVYG
                     ISAFSLSQDIGVSVKEADSYIKGYLETFSGVRNYMERVVKEAYDKGYVETLLGRRRML
                     PELTSSNRNIRNFGERVALNTPIQGTAADIIKIAMVRVDERLRREGLKSRLILQVHDE
                     LIVEAPKDEAVTAAEIVEQEMENALPLSVKLEADVHRGDSWFTAKG"
BASE COUNT         4252 a         4298 c         3639 g         4070 t
ORIGIN      
        1 acccgttacc tacttcgttt acttactttc tttcctactc gggataagct tccggtggat
       61 gggtcaaccg gtatttgccc ggatataaat acaaaacctc ccgcctctac ggcctgagaa
      121 tacggaccga tggctgccgg cgcattttcg gtgctgaaaa tctgcttcat ttttcatttc
      181 ctttccaagt tttcaagcgg accaatccgc ttgttttcta cttattataa catgttttac
      241 acaataataa caataatcta ttatggcaat aatttattat agaagttttt tgtatcaaat
      301 ctcatttata gcacttcgtt atatttataa tgaaccgtat aatgattaaa attccatttg
      361 aagtaaacct tccaaaaaag gacgcggaac agcaaaaact gctccgcgtc ctttcatgtt
      421 ttaagtttga tacagattgc aaagatcaga tgatgccata gaaaaccggc ttatttaaaa
      481 tatcttttca aaaggccgtt aaaagctgct ccatgccgag cttcatcctt acacatttcg
      541 tgaacggtgt catgaatcgc gtcataattg agctgttttg ctctgaccgc cagatctttt
      601 ttgccctggc aggcaccaaa ctccgcttcg actctcatct caagattctt tttggtgtcg
      661 ggaagaacaa cttctccaag catctctgca aatttcgctg cgtgttccgc ttcctcaaaa
      721 gcgattcttt tataagcttc cgcaatttcg ggataccctt ctctgtccgc ctgacgagac
      781 atggccaagt acatcccgac ttcggtgcat tctcccgtaa agttggcctt cagaccctca
      841 acaacctcag ggtcaacgcc tttggctaca ccgatcttat gctcatccgc ccaagcgagc
      901 gcgccatctt tcttttcgct gaatttgtcc ttcgtcgcgc cgcactgggg acatttttcc
      961 gggggctctt cgccttcata aacataaccg caaacagcac agacccactt tttcatacaa
     1021 attcctcctg agttttattt ttttcatgtt agagcttttg gtaatggtct ttttgaatct
     1081 gaacttatca tacaacaaat ttttctatat ttcaataaac attactaatt ttgtatactt
     1141 ttttagatat tcaaaacagt ccataatccg atatttctga ttttatttgt accaaattca
     1201 atcaaaataa gaactgtgtt gaagtgtagg gaatttattg cttgcatttg gtcattttct
     1261 atattaaact atatttagta tattataggc ttttatttac attatttgta gtttatgaag
     1321 aaaaggagaa atacggtgcc tgaatttgta tatacgtcga aagatcaaac tggcaaaact
     1381 tatagcggag tcattgatgc agattcgatc gacagctttt accatattat aaaagacagg
     1441 tcgcagtact gcatcaccgt caaagagtcc ggcgtaccac aaaaggagat cgtattcggc
     1501 agcaaaaagt ttaacctcaa ggaactaagc gttttctgcc gtcagttcag cacgatgctg
     1561 aattccggtc ttcccgtcat taaatgtctg gatattctct accagcagac caccaataaa
     1621 aagatgcgat cgtccatcct cggcgtttat gagacggttc aaaggggcga atccctttcc
     1681 agcgccatga aacatcagaa aaccgcattt ccgtcattgt tcatccattt ggtggaagcc
     1741 ggcgaggcaa gcggttcgct ggattcagtt atgaaccgaa tggcggagca gtatgagaag
     1801 gaaaacaagc tgcagaacaa agtgacgcag gctcttattt acccgatttt tctctgttgt
     1861 ttatcaattg ttgttgtaat ctttcttctg accttcgtgc tgccaacgtt cctatcaatg
     1921 ttcacgcagt ttggaggtca gcttccggtt acgacaaaaa ttcttctggg aatcagccat
     1981 ctgttcacca attactggta cctgatttta atactgctgg ccggtattta tatcgggttc
     2041 agagctttga tgagaaaaca taatgtcagg ctcgggtggg acaagctcaa gcttcgtctt
     2101 cccatcatcg gaaaactgct tctgattttg gaatcctccc ggtttgcacg tactctcgcg
     2161 tcattgttca acagcggcat gccggttatg cagtcggttg aaatcaccgc aaatgtcatg
     2221 acgaactctt atatccgcaa cagactgttc catgtcaatg aagaagtccg cagaggttct
     2281 tcaatttccg catcaatccg aaagttgaat ctttttcctc tgatgctctg ctcgatgctg
     2341 aacatcggcg aggaatccgg caatatggat gtaatacgtc ttaaaacctc cgccttttac
     2401 gacgaggagg cggacgcggc gttgcaaaag ctggtttcgc tgattgaacc atttatgatc
     2461 gttctgctgg ccgtcgttgt cggtttcatc atcgtctcca ttatcactcc aatttacggt
     2521 gtctacagcc agatcagtaa aaccggaatg taaaaaaggg aggttaacta tgttattgtc
     2581 aatcgacgtt ggcagcgtaa gcttatacgc cgtacagggc tcctgctccg ccggagctgt
     2641 cagtatcgac agggccggtg agatcgcgtt gcctgaggtt actattgagg acggtatgat
     2701 taaaaaccat gcgtcattta taatgaccgt caacaaactg ctcaccctgt gcaggtttac
     2761 tgcaagaaac actgttttta ccatcacgag cagttcggtt ttatctcgca ggctggagct
     2821 gcccgcttca acgccacggg aactgaacat gatggtaaac aatgaaatga tacaggtcgt
     2881 caacgattcc agcgactttg tctacgaata ttccataatc ggcccggagc atgcgtccaa
     2941 acagaaaact attcccgttt gggcatacgc gctgcctagg gattgcgtcg accaatatta
     3001 ttctgtcttt aatgggataa gactaaaacc gaaagcactc gacattcacc caaattcggc
     3061 agaaaaatta tttgtaaatt catcggtgaa tggaaatgaa atcgggcaaa aatcaatact
     3121 ttttgccgat atccggcaaa atgacgtgga aatccatctg atcagcgacg gtcagcgggc
     3181 attttcccgt acttctcccg tttcttcttc aacttttgaa gcaattttga ataattccgg
     3241 tctcgtcccg gaaaacgtca gtatgtttga ttcattggat attacaagcg ataaattccg
     3301 tggcaacagc atcctaaatg acgcggccca tcaatatatc aaccagattt gcgaagaact
     3361 tcaaaaaatg gtccaattcc agcttagacg gaattccaca aacccggtcg ccggcgttta
     3421 catttacgga aacatgtccg gaatcagagg tttgacggaa gggatgagct ctcttctcgg
     3481 aatccatgtt gagaaagtgg aagctgtctc gaaagtgaaa gcaggaaatt tagaaattgc
     3541 caaatatatc aacgcaattg gggcattgat cagactgtga ggtataatat gcaaagagat
     3601 attaactttt tctccgttta ccattcctcc gccgatcatg gcggagttga ccgcactacc
     3661 ctgataggcg gaattgtcac catagccagc gtggtcatcg ttttgggagc ctttcttttg
     3721 ctaaaaatcg gaacagctgg tttgtctatg caatcccaga gcatcagcca gttcctcgcc
     3781 cggcccgacg tcagcgcggc cacctcaaga gtcaacgaaa tctacgcaaa aaacaattac
     3841 ctggataaat accaaagcac ggcaagctcc aacatcaaga aattttatgc cctaacaaaa
     3901 tttgattcca aactgattga tctgatttcc aaagctgccc cggcggacgt cactgccagt
     3961 gatctaaatt ttggcggaaa catcgtgtca ttgacctgca ccgcaacaga cgagcggtcg
     4021 cccgcaaatt ttgttcacac cctgtctgcg accggttctt ttgagcaggt tacctatgac
     4081 ggtgtgataa aggattccaa cagccaatat actttctcgg tggattgcat tatgaaggga
     4141 gaaagcgaca aatgacgtta acttcaagag aaaaaaaact cctgttcgtg ttggcgcttg
     4201 tcgtcattct tgtcggaggt tttcgctttc tggttctgcc ggcttacagt aagttcaaca
     4261 ccgaaaatga gaatttttcc acccttctcg ccaggcagct gatcgccaaa ggaaacctcg
     4321 ccgctgcggg aacgatcgac agcggtctgc aaaaaaagct gagcgacgtt gactcgtcgg
     4381 cttccgcgct gtttccttcg ctgagtaacg accagctgaa catctggatc ttagacctga
     4441 ccgcaaaaaa cgggctgagc gtaagctcga tcaaattcgg atctccttat gtaacagaga
     4501 tcagtaaaga caacatcaaa aacgccgaca gctcaacttc cggctccacc agcgcgggtc
     4561 agaaccagac agccgccggt gatctcaatt atctgcttaa aaaatacgca cagggttata
     4621 aaggcagctc aacctccgca gtgccagcga cgacgtcggg gagctcttcc tcatcttcgt
     4681 cggcacaaag cggcgcatcc tcccagcagg caggaaattc gctggttggc gaacagataa
     4741 aaatctcgat gaccggaagc ttcgctcagg cgaatagctt tcttgatgcg gttaaaaatt
     4801 ccaagaaaac cgttctggtc tcttctttca gctgctcacc gaagggcagc acgctgacca
     4861 ttgacgccac actgctgttt tacggcgcgg aaaaaccaga tagcagcgac aagacgttcc
     4921 agtagaacct gtcgaaaccg gcggggaaaa acggcctgat gtaagcccgc aaacgggcca
     4981 tacgaggtat taaatatgaa ttgcagacaa gggaaaataa cctccaaccg caacagcagc
     5041 ggcagctcac tgatcatggt gctgattatt ttcgccatgt tcctgatgct gtccgccgca
     5101 ctgctgtcgg cggcgtttac ttcgaccgca atggccggca aacaggttac cagccagcag
     5161 aactatctga gcgcccgaag cgccaacgcc tcccttttaa aggtctttac ggattccacc
     5221 aactccggct ggcaagcact taaggataaa atacgtttaa tgaccgtcgg cgataccctg
     5281 accggtgaaa tcaacgatcc caacctgggt catattcgaa tcgtgatcac caaaaccgga
     5341 agtacgacta ccggtatgac gtttgatatt aagtccacta caattttatc cgacggaacc
     5401 caatccgcac cggtaatttt acatctcagt gcaccaatta cctcgagcgt tgtctatgat
     5461 gcctccaacc cctacaattc attattttat tacaccggca gtgttacgca ttatatggag
     5521 accggcaacg ccacgggaat gctgatttat aacggaccag tttatatcgc ccaggcaggc
     5581 ggcagcaaca acaacgtgaa aggtaatgtt ttctcgaccg acgtcattta tctcagcgga
     5641 agccaaaccg gcatcacata tctggcttcc aacaaggata ttttcttaca ggatacctat
     5701 aaactctcaa gtaacggtta ctactattcc gataacaatc ctgtcatcgg aacatccgcg
     5761 gtaccctgcc cggtcatcgc aaaaggctct ctcagcgtgg gagggcatta caattctaac
     5821 gactacccga ttatttatgg caacgttacg gttgacggca acgtcacact gaccgatcat
     5881 tgcagaattt acggaaacat aacggccgga ggcgccgtca ggatcgacgg cggctgcacc
     5941 gtccaaggca acgtagtagc cggaggcggt ataaccggct ccggagcagt aacgggaacc
     6001 aaatcgccca acacgaagaa tatcccgaaa gtcacgatta tctatacccc caccgacaaa
     6061 ttgccggcaa taaccccgcc cgcctacagc aacgcggttg cggtgggctc aggcggaacc
     6121 atcaacacca gcggggtgct ttcctcctct tctttcaaca acagcgacac gctttatgtc
     6181 gacacgtcgg ccgcagatgt taatctggtc ataaaaaacg atctcaccct aggctattcc
     6241 gattggtgga ataattattc acagaaaatc ctggtcagcg gctcacacaa cctttatatc
     6301 tatctcgcca gcgggtcaga tcttaccctt cgaagttcgt ctcaaatcgg aatgcgcgac
     6361 ggtaccacag gcaacgtctc ctccagcaca agtcagagcg gctggcccca tattttcatt
     6421 atcggaaaca accagacgct tacgcttaac gacacctcgt tactccgggc caatatcttt
     6481 ctgcccaatg gcacttttgc cggcaattgg tcgaatgggg ccagcagcaa taaccggttt
     6541 atgggcagcg cgctgatcgg agacgacaca attatcgtat caagcaagag tcagagcctc
     6601 tacttcttga aaccaacatt ggacaacacg cctctgtcgg gctttggtac tcaaaaatac
     6661 gcagtgggga attatacggg gagctggtcg aaatgaaaaa gtttatcaga aatcatcttc
     6721 gcaatcaacg cggctccacg ctggtggaag tgctggtcag ccttgctctt ttcgcaattg
     6781 tcgcaatctt tattctaagc acgcttctga ccggaatcac catcgtaagc tcggcgaaaa
     6841 gcaagtcgca gaacagcatg aaaactgccg gcggcctcgc tcaaaaaacc gagaatcaaa
     6901 ccgtctccga cccacaggga accacggtca gctcatcaag cggcaatttt actatgactc
     6961 tgaccgacag cgccgacggc tctcaaatca ccgtaacggt ccccggaagt ttcctgaccg
     7021 ggactaccag cggcaagaat cccggagaaa gcgtcactta taaaagtttc agcccatccg
     7081 acagcggctc ataaaggcgg tattttatga aacagcgatt aaaatcccaa aaggggctaa
     7141 ccctcgtaga gctaatgatc tcgatggccg tttccatgat tttgttagga gcctgcgcgg
     7201 gattactggt ttatggattt aacctatata acaaggccaa cgattacaac caggcccatg
     7261 tgttagggct gctgacgcag cagaagatcg aaagtgaagt caaatacgcc gcttcaatga
     7321 agatagacgc ctcacagccc ggctcacttg caaacgggaa ctattgtctt gcgctcaccg
     7381 gcagcggacg tcttgccaaa tacaccggca cggcatcggc tatgacgagc aactacgtct
     7441 atctcgatgg cgtctatcgg ggatacgtct gttccatcac cttcaaggca agcactggtt
     7501 ccacgctgaa cgttgaagtt accgtcagca agggcggcga aaccataacg gacacaacaa
     7561 cggatattat cctcaacaac atggggaaaa acattccgtc agggcaggat atcaacgcga
     7621 ccgttacaca gggaaacgcg attgaattta cccttccctc ggcctgaacc cgcggctaca
     7681 gattttcact gcccgccggg tgaccaaaga aagtatggtt atatggtcat ctccaaatca
     7741 aacaatttaa atagcgtttt tcagcaatat ctcgactatc catgccatga acggcttgat
     7801 gcggtagccg aagcagggcg tcctctggtc atacatttcg cttccctcta cggctccggc
     7861 tgcccgtttg acgacattta tcagaccggt atgctcgggc ttgtcaaagc cgtcaaaacc
     7921 tacgacggaa gcaccctctt taccacctgg gcctccaact gcatcattgg ggaaatccgc
     7981 cattttgttc gaaaagaacg aaattaccat tatccggact gtatcggcca attacagcag
     8041 caggctgatt tactcattga aacggcggtg aaaaccgacg gcccgccgcc ggataccgga
     8101 gcgcttgcgc agaaactcgg ggtcagccgc gaaggctatc gggaagtcat gcgtgcgggt
     8161 ctggtctcgt ttaacgaaat cgacgtatca aaaatcagct cggcccaagc ccagtcgttt
     8221 catttaccga ttgaggataa gatcgcgctt gatcaggcga tcggccggct cagcgagatt
     8281 cagaaacacg ttgttcacgc tttgtattac cgcgggctga cacaacagca gacagcggaa
     8341 gagctgggga tcaatcagcg caaggtcagc cgaatcaaaa ccggcgttat ccggctgctt
     8401 ggcgaaatgc tcaacgaacc gaatttccgg cttattgaca gctccagatc ttttgttcct
     8461 atcaataaaa aatcaaaaaa atagcccggc ttctaaagaa gccgggcttt gccttcaccg
     8521 tcttgttata aggatcaggt actatgactt ggtatatgtg cccgtccgac cattgctttc
     8581 ggtaactgta acaattgtcg gactaccaga tggaattgtc accgcaatcg aaaacgaatg
     8641 agttcccaaa tatgtgttta gatcgtcttt ataaattgtt gtttccgaag ccccagttgc
     8701 aggtttattg ctatcaacat atgcagtata agtgacatcc gaatgcgccg ccgtatatgc
     8761 ggagactgcc gaatatacgg tacgcgcatc agcagtggcc acactatccc tcgcggaatt
     8821 gatataacct gataccgtcg ggatcaggat agccgccaaa ataccaagaa tcgcgatgac
     8881 aacaagtagc tcaataagcg taaagccttt tttgtctttt ccaatttttt taatcaacgt
     8941 ttgaatcata taaacatcct cctttatttt ctcataatgt ttatacctca aatttctgtt
     9001 tttagacgct ttttgcaaaa tcttctaaat atttagatat cttttgaagg tatcgacatc
     9061 catgcagtgg gaaagcgcct cgtcggacga aatgctgccc tgacggacga gccggctgag
     9121 gtggtaatcc atcggcatca tgccctgttt gattccggtc tggagcgccg aagtgatctg
     9181 gtggcatttg ctctcccgaa tcatgttgcg tatggcgtca ttgaccgtca tgatttcgag
     9241 cgcggcaatt cggttactgt gagtagtatt cgtaataagc tgttgcgtta ttacagcttg
     9301 taaaacagat gccagctgaa cgcggatttg ctgctgctga taaggcggaa acatatcaat
     9361 catccgatca accgtttgcg ccgctccgat ggtgtgtaat gtggaaagaa ccaggtgacc
     9421 cgtttcggcc gccgaaatcg ccgttgcaat cgtctcgaga tctctcatct caccaacaag
     9481 aatgacgtcg ggatcctcac ggagcgcgga acgaagcgcg ccggaaaaac tgctggtatc
     9541 gtcaccgatt tcccgctgat tgacaatgct gtttttatgt ttatgaagat attcgatcgg
     9601 gtcctctatg gtgagcacat ggcagcgctt gttgctgttg atatagtcaa ccatggccgc
     9661 cagcgtggtc gattttccgc ttccggtcgg cccggtcagc agaatcagcc cccggggcct
     9721 gagcgtgagc tcctttaata tctccggaag ccccagtgac tcaatggtcg gcatttgctg
     9781 aaccagcacg cggatcgccg cggaaaaact acgccgctgt ttataaatat taacccggaa
     9841 ccttccgact ttcgggacta cataggaaaa gtcgacctgt ccgttttttt caagaacatt
     9901 tttctgttcc tcgccaagca ttgccatgca gatcgttttg gtgtcttccg gacgaaacgg
     9961 ctcatagcct tccagcggct gaatctggcc attgattcgc accatcggcg acacaccaac
    10021 ggtcacatga agatccgagg cacttttttc gattgctttg atcagcaatt cattaatcag
    10081 atccaaagtt ttttcccccg tcaaatatta taagtggccc tcatcatttc ttccgtagtt
    10141 gtcaaacctt ttaacaccag ctccctgcag ctttcctgca gcgttaccgt cccgcttttt
    10201 gtcgaaattt ctctgatttc atcggccgac gcaccgctct ccacgagttc cctgattttt
    10261 tgagacatta cgataatttc atgaacggcc gcgcgcccct tgtagccggt gttgtcgcaa
    10321 taattgcagc ctaaaccgtg ccgaagcttt atgggcgcag tgagcccaag ctgccgcatt
    10381 tcctcctgcc cgggaagata gtctactccg catttcgggc agaccctttt caccagtctc
    10441 tgggccacga tgcctgtgac ggcggaggaa accaaatagg gctcaacatc catattcacc
    10501 agccgcgata cggtggatgc ggcgtcgttg gtgtgaatgg tcgacagaac aagatgcccc
    10561 gtaatggcgg cccttacggc aatctgcgcc gtttcagaat cccggatttc acccaccatg
    10621 ataatatccg ggtcctgacg gagaatggaa cgcaggccgc tggcaaaggt aagccccgcc
    10681 ttcgggttta cctgaacctg attgatacca ggcatccggt attcaaccgg atcctcaatg
    10741 gtaataatgt tgacgcctgg gtcgttgaaa gtattcagca tggagtaaag cgtagtggtt
    10801 tttccgcttc cggtcggccc gcagatcaga ataatcccat tggaattttt caaaatctgc
    10861 tcaaaaagcc cgcggttgcg ctcggacagg cccagatcgt caacatccag cacggttccc
    10921 gtacccccga gtattcggat aaccaccttc tccccggtaa cggtcggcag aaccgaaagc
    10981 ctcagatcaa ccggctgacc gtcgattacg gtttccaccc ggccatcctg aggaattctt
    11041 cgctcggcta tattcatatt gcccataatt tttaatctgg tcacgattga agcatgcgcg
    11101 cccgccgcaa gggtcatctg gtttctcatc acgccgtcga tccggatacg aaccctcgtg
    11161 gattcctcca tcggctcgat gtgaatatca ctcgctccaa gtttgactgc ctgctgaatc
    11221 atggaattca ccaaacgaac aaccggggcg ttatcgaccc tctctccgac ctgctccgaa
    11281 agctcctcaa tttcccgacc gtcaaattcc cggctgagat ctttggctgc cttctccgcc
    11341 tgcgtacggc tgtaatactt tccaatcgcc gccttaatat cggaatagga agcaagcacg
    11401 accctgacct ccatattggt aacaaggcgg acatcatcca aggcgtaaaa attcatcggg
    11461 tcactcgtcg caacggtaag gtactttccc tcaaccccaa cgggaataat gttatattta
    11521 acggcaatat tttccggaat tagcgcggga gcttttgggt cgacagcctc gacgtttaaa
    11581 tcaatcaacg gaatcttaaa ccgatcctca agcgccttca tctggtcacg ctcgccgagc
    11641 gcgcccatat ttaccaggat ctctccaagt ttgcgtgttc catcggtttt ttgtatttcc
    11701 agcgcctgat caagctgctg ctttgtaatc agatttgcct caattagaat ttggcccagc
    11761 ggcacatttt taaaaggcat aatacacctc atgtacattt ctttcgtttt tctatgattg
    11821 ttggtttttt gaattaactg ttacaatcgt cggcattgtg agactcccgc tgtttttgag
    11881 ccggcggtaa ttcaacacgg ccggaacaag gagaaataaa aattgagaat cacgttgcca
    11941 aacaacatgg acagcccgaa acccagtgaa agaaaaggcc caaacgggat ggatgtctta
    12001 agcgattttt ttgatttgac ggcataaaca acgccaaaca gggcccctgc cacacttcca
    12061 agaaacaacc ccagcagcgc cagcttatag ccaagaacaa gccctgccgc cgccgcatat
    12121 ttcacatcgc ccattcccat cccacctttt gaaaggatgg ccgctgctag gagcgggaca
    12181 ctaattgaaa aaaaaccaat tattctttca taagcaggaa tattatggct aataatcaga
    12241 gcgatcacgc cgataataat tatatatagt acaattgaat cgggaataat ggatttgtca
    12301 aaatcgataa atgctattac aatgtaagca gagactgcag ccgcgtaaac gaaggacagt
    12361 gctgtcgcac cgctgaatgt aaaaatcagc acataggcaa ccgcattcag catctcgacc
    12421 agagggtagc gccaactgat ttttgctccg cacgatctgc acctgccccg caaaagaatg
    12481 aaacttacga ccggaatcaa atcgtaatcc ttgattgcat tgccacagga ggtacaatgg
    12541 gaacggcctg ttacaatcga ttcatgaacc ggtattcgat aaatgcagac gtttaagaaa
    12601 ctgcccaacg caaggcccgc cgtaaacaca aagaaatatt caatcaaaat tagtatgttt
    12661 tgcatacttg cctactctca acattctttt tcaatgattc atactatccc gtataatgct
    12721 attttatacg attttccaag ttcttgcaag aaccggctgc tatattcctg gacgacattt
    12781 gatttgcatc attaataacg taatcccaac ataggtagac taagcaaaag gaaagggatc
    12841 atttgtgcac gaatttgata aatgcatcat cacaaacaaa aatggagcta agatttcgga
    12901 aggaatcgtc agtgaatata attccgccga tatgaggatt aataccacag aatcccacaa
    12961 tctgagagtc tatgatgaag ttctcatcta tgtttataac caactcaaag gcgaatgcaa
    13021 atacgagggt acggtcagcc aagcaaccgg aagccgcatt accctgttta acgtcagtct
    13081 cgttcgttcc gttcaaaaaa gggataacac ccgggtaagc aaacagctca agtatatggt
    13141 gactcacagc ttcagaggcg accgagccat caaacttgag aaaccaattg aaattaccgt
    13201 tttgaatatc agcgctcagg gtctttattt aaacagtacc catgatttcg ctattggcag
    13261 ccgtttcccc cttgtcctgc aggaaaccat caaacctatc aaagtaaccg ttgaaatcct
    13321 gcgcaaggaa aaatacacca gaagcttcaa ttatggctgc cagtttgccg atatcagtca
    13381 aaaagatatg gatgaaattt tccgctttgt tttaaaagaa cagattgacc agagaagacg
    13441 aaacttactg tttcatccct gaccaccttc ctgaactgtt caacccccgc tgatgacagc
    13501 gggggttcta tttttcttca atcaatctcc atattggcgc aggcgttaag gtccgtgccc
    13561 gccgtatggc cggacaccca ttcgtaatag ccctgacatc cgaccattgc cgcattatct
    13621 ccgcacagcg aaagcggcgg cataaacagg ctgaatcccc gccgagagca ttcgtccgtc
    13681 agcctttccc gaagaccgct gttcgccgca actcctccgg ccaccacaat tttacggctt
    13741 ttcgttgcct cggccgccag catcagcctg tcgctgagga tatctacaac cgcttgctga
    13801 aatgaagcgg cgacatcgtt tggattgagc tgctccgatt tttgcgaagc gttgtgaaca
    13861 agattgatta cggctgtttt taaaccggaa aaactaaaat cataaggcga gccatccacg
    13921 tgcggccttg gcagagccac ttttttcggg tctccgagct tggccgcccg gtcgatatat
    13981 actccgccgg ggtagggaaa gcccagtgac cgcgccgcct tgtcaaaagc ctcgcccgcc
    14041 gcgtcatccc gcgttctgcc caagacccga aacgaggtat agccggaaac ttccaccaga
    14101 tgactatgac cgccggaagc cacaaggcat aaaaacggcg gctcaagctg aggggaagcg
    14161 atataattgg ccgcgatgtg cgagcgcaga tggtgaaccg gtatcagcgg cacatccatt
    14221 gtcagcgaca gcccctttgc aaaattcacg ccgaccagaa gggcgccgat caaaccgggg
    14281 gcgtaggtaa ccgcaacccc gtcgatgtct ttaaaatcac agccggcttc ttcaagggcc
    14341 tgacgcgtaa tcgaggcaat tacctccaca tgcatgcggg aggcgatttc aggcacgacc
    14401 cctccgtaaa tccgatgggt ttgaatctgg gaagaaacga tattggaaag aacttttctg
    14461 ccatcctcga cgaccgcggc tgcggtctcg tcgcaggacg actccaaggc aagaatcttc
    14521 aatgtatgca ctcccgatca ttttgttgaa tcatagaatt ttgtcataat cacggcatcc
    14581 tcacggggat tcgcgtaaaa tccgggccgg attccctgtt cccggtagcc tgacgcatga
    14641 tacagcgcaa tcgcaggccg attggaaacc cttgcttcca gcgtaataaa ctgaaggctc
    14701 ttcttttggg cgtatccgtc aagccagtcg agcaaagccc gcgccgcgcc tctgcgccgg
    14761 aaccccggat ctacggcaac attggtcaga tatccttcat ccagcacatg atacattccg
    14821 gcatagccca ccgtcacgcc atccagctcg gcaacaagaa aaaccgccag cggattttca
    14881 atttcttccg ccagaccctg caccgtccac ggctcggaaa aacacagtct ctcaacttcc
    14941 gcgagttttt caagatgctc cggccgcata gggacaattt ccacggcgga gcgatcctcc
    15001 gtcacacctt tccctcgtta cggcgtttta tctggccgac gacaggccga atcctttgga
    15061 tcaattcatc ccggcaccgg cgatatactt caatacccgc tccgtaagga tcgggtattc
    15121 cccccagatc aacaatttta tctctggcct gcgggtaact tgccactatc gccattttgt
    15181 gggacggcga catgacataa atccggtcag cctgctcgat caattcccgg ctcagaagtt
    15241 tggaacgatg ccgggagaga tccaagccgg cctcgccggc cgccgccacc gcgttggcgg
    15301 atgcgggcgt tccctctgcc gcggcaattc cggcgctgtc ggcaacaacg tcggttatac
    15361 cgttttcctg aagcatttta ttcatcagag cggaggccat ggggctccgg caggtgttcc
    15421 cggtgcaaac aaacaacagc ttcttcatat ccgctttttc tccttatccc ttggcggtaa
    15481 accatgagtc gcccctgtgg acatccgctt ccagtttgac actaagcggc agggcatttt
    15541 ccatttcctg ctccacgatc tccgccgcag tgacggcttc atctttcggt gcctccacaa
    15601 tcagttcatc atgaacctgg agaatcagcc gtgatttcag cccttcccgt cgaagccttt
    15661 catccacccg gaccatcgca attttaatga tgtcggcggc ggttccctga ataggagtgt
    15721 tgagcgcaac ccgctcgccg aaattgcgga tatttcggtt ggaggaggta agctccggca
    15781 gcattctccg ccggccaagc aatgtttcca catagccttt gtcgtaagct tcctttacca
    15841 cccgctccat gtaatttcgc acaccggaaa aagtctccag atagcctttg atgtagctgt
    15901 ccgcctcttt aacgctgaca ccgatatcct gcgaaaggga aaacgcgctg atcccgtaga
    15961 cgattccgaa attaaccgct ttagcccggg aacgaagtac cggggtcacc atttccggcg
    16021 gcagccggaa aacctgggaa gcggtgatcg tatgaatatc cgcgtgactg ttaaacgcat
    16081 cctgcatcgc ctgatccccg gcgacatggg caagaatccg cagctcaatc tgcgaatagt
    16141 cggcgtcaat caattcgcag ccctgggcgg gaatgaaaaa gcgcctgagt tcccgccccg
    16201 cttcgctcct gaccggaata ttctgaagat tcggttcggt ggaactgatc cgtcccgtc
//
LOCUS       DATALY010000184        10605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_729255,
            whole genome shotgun sequence.
ACCESSION   DATALY010000184 DATALY010000000
VERSION     DATALY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729255"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="argF"
                     /locus_tag="VHR42_05350"
     CDS             <1..690
                     /gene="argF"
                     /locus_tag="VHR42_05350"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003395714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX3026635.1"
                     /translation="IEYRGYDQSIVEDLAKYSGVPVWNGLTTADHPTQVLADFMTIQE
                     HFDKPLNKIVFVYSGDGRNNVANALMIGASKMGMDFRMVSPKELFPTEELLKKCREVA
                     KETGAKITCTDNIREGVKNADVLYTDVWVSMGEPDEVWASRTKLLKPYQVNQEMIELT
                     GNDKVIFEHCLPSFHDQKTKIGKEIYEKYGLKEMEVTDEVFESKHSVVFDEAENRMHT
                     IKAVMVATLGE"
     gene            724..2157
                     /locus_tag="VHR42_05355"
     CDS             724..2157
                     /locus_tag="VHR42_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005275 - amine transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HEX3026636.1"
                     /translation="MERTNDEGGSKKLGLFLLVMLIVGSIIGSGIFNSPTDLINKANP
                     LATIIAWLVGGFGVLMLVLMFQMLSTKKPELKGGIYSYAKAGFGDYVGFNSAWGYWLS
                     AFLGNVAFITMVFKTLNSMLGDKHQLSSFATFLLGSILLWVYHLIITRGIKQATVLNA
                     VITIAKFVPLILVIVFGIAVFSSGTFTVQNWMSNLASTGESTSLGAQINNAMGTILWC
                     FFGVEAAVVMSNRAKSSKLVGKSTVISFLIVLVLYMLISIISMGVVPAKQLAGSATPL
                     ADVLSKTFLGSAGSVIVHLGILISILGALVSWLMLTAEVPYIASKDGVMPKWFSKENH
                     AGVPINSLIFSNGITQLFLLFLLIPQLQTAYSAAYTLATTAILVPYLFSALYALKITI
                     TEKLNAGCLAISVLATLYSVYVIYAVGLKYLAAAVILYAIGGIPFYFARREKSQSLSR
                     NERIALGIIVMIALAMIVMIATGVIQL"
     gene            2455..3405
                     /gene="arcC"
                     /locus_tag="VHR42_05360"
     CDS             2455..3405
                     /gene="arcC"
                     /locus_tag="VHR42_05360"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX3026637.1"
                     /translation="MARIVIALGGNALQSDPKDISAKAQQQTIESISGVLTDLIEKGH
                     EILFVHGNGPQVGQIMSSYETSATVNPLLPVMPFPECGAMSQGYIGYHLQQTLKNAMV
                     RRGIKKEIVTIITQTVVDKNDPAFQRPSKPVGSFYTKEQAKELETTRGYVMKEDANRG
                     YRRVVPSPKPLEFVEEPIISALLDAGHLVISAGGGGVPVIDRGNGELEGVPAVIDKDF
                     AACKLAEDIHADALIILTAVDNVAVNFGRPDQKNLTNLTVSEAEAFIAQGQFAPGSML
                     PKVEAAISFVKSEPNRKAVITSLCQALEAIGGKAGTVVTI"
     gene            complement(3901..5472)
                     /locus_tag="VHR42_05365"
     CDS             complement(3901..5472)
                     /locus_tag="VHR42_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3026638.1"
                     /translation="MKPIPFSSLNLTSEIQRATHAMGFENATEIQAKTIPLILEGKDV
                     IGRSQTGTGKTASFAIPAVEMIDVDHKFQVQVLVVCPTRELAIQSWGEFKKLYQFKSG
                     IKAAVVYGGQPIDRQIKELKKGVNIVIGTPGRIMDHLRRKTLKLNHLKMVILDEADEM
                     LNMGFREDIETILSQAPEERQTVFFCATMPPEIMAITKTYQKHPELVEINRNQVTLEA
                     ISQYYFETSASGKQEALAGLLKSENPGRAIVFCNTQKMVDGLVIFLVRQGFEAVGIHG
                     RITQNVRTRVMEKFKAGRVPILVATDVAARGIDAQDVEMVVNFDIPADVEDYIHRIGR
                     TGRAGKSGKAYTIISGRKQILQLRNIERVTKSSIIREEVRTGGIVQRQKPDSAEVSHP
                     EKDRKRRKAVIDFAREFSPSKRERPSPKDGSSRIILNIGKMQHISPNHIVGAIAEKTS
                     LSGSDIGKIQVLDNSTIVEIPGAQRQEVIDSLKGQKIKGMFVDAKPHDREEPRRAPSG
                     YASHRFKDRQPGRRG"
     gene            complement(5881..6069)
                     /locus_tag="VHR42_05370"
     CDS             complement(5881..6069)
                     /locus_tag="VHR42_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX3026639.1"
                     /translation="MKATIDRSGCISCGLCPEVCPEVFRMAEDGFAEVHAEEVPAEAE
                     AKAVEAQESCPVSVIAVE"
     gene            complement(6272..6940)
                     /gene="thiE"
                     /locus_tag="VHR42_05375"
     CDS             complement(6272..6940)
                     /gene="thiE"
                     /locus_tag="VHR42_05375"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX3026640.1"
                     /translation="MKPDLDCSLYLVTDRNLMRTPTLSLAVEQAIQGGCTLIQLREKT
                     VSSLEFYRLAEEIKIVTDRGRVPLVINDRVDIALAVGAAGVHIGQNDLPAGVVRSLLG
                     TDQILGVSVETPEQAVRAERDGADYLGVGAMFATGTKSHAGIVTMESLKVIRAAVTIP
                     IVVIGGIGPDTIPLFDQTGIDGIAVVSALMAADDIARSARELKKEFSKLTIPSEAGAV
                     DTKR"
     gene            complement(6930..7757)
                     /gene="thiM"
                     /locus_tag="VHR42_05380"
     CDS             complement(6930..7757)
                     /gene="thiM"
                     /locus_tag="VHR42_05380"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEX3026641.1"
                     /translation="MKLGILAEIIKNMEEIRRKKPLIHNITNYVTVNDCANAVLAIGA
                     SPVMADAAEEVEDIVAISSALVLNIGTLNRRTAESMLTAGKKANELQIPVVFDPVGAG
                     ASGYRNRVAERLLTELKITVLRGNSSEIRSVAGFCAETKGVDASEADLLTGIRAGKEV
                     AMKAAEQFGCVTAVTGPTDVICDGKRTVLIENGSRLLSGVTGTGCMCTSLIGAFCGAG
                     GDPFAAAAGGVLSMSLAGELAQEIGAGGNGSYHLSVIDQIGRLNAETLAGRAKIHEA"
     gene            complement(7754..9097)
                     /gene="thiC"
                     /locus_tag="VHR42_05385"
     CDS             complement(7754..9097)
                     /gene="thiC"
                     /locus_tag="VHR42_05385"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX3026642.1"
                     /translation="MNSNTQMKAARNGIVTRQLAQVAKDEGISERELMELVAAGTTVI
                     PANIRHHSLHPFGVGRGLRTKINVNLGISGDCADYRAEMEKVELAVRYGAEAIMDLSN
                     YGKTREFRKKLIEKSGAMIGTVPVYDAIGYLEKDLTEITAADFLRVVRSHAEEGVDFM
                     TIHAGLNRRVAAVFEREKRQMNIVSRGGSLLYAWMKLTGNENPFYEHFDELLEILCEY
                     DVTVSLGDALRPGSIHDSTDAGQIAELTELGVLTKRAWAKDVQVIVEGPGHMAMNEIA
                     ANMTLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGAIAAANGADFLCYVTPAEHL
                     RLPDLSDVKEGLIASKIAAHAADIAKGVRGACDADYKIGEARRTLDWEAMFSLALDGE
                     KARAYYESVPPEDKHSCSMCGKMCAVRTMNRILSGQDMDLKPESKREKAERNESK"
     gene            complement(9096..9905)
                     /gene="thiD"
                     /locus_tag="VHR42_05390"
                     /pseudo
     CDS             complement(9096..9905)
                     /gene="thiD"
                     /locus_tag="VHR42_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
     regulatory      complement(10042..10140)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      complement(10268..10366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         2531 a         2878 c         2506 g         2690 t
ORIGIN      
        1 atcgaatacc gcggatatga ccagtcgatc gtcgaagatc tggcaaagta ttcgggagtt
       61 cccgtatgga acggcctgac cactgcagat catccaacgc aggttctggc ggatttcatg
      121 acaattcagg aacactttga caagcctctg aacaaaatcg tgtttgttta ttccggcgac
      181 ggaagaaaca atgtggccaa tgcgctgatg atcggcgcgt cgaaaatggg catggatttc
      241 agaatggtat cgcccaaaga gcttttcccg accgaggaac tgcttaaaaa atgcagggaa
      301 gtcgcgaaag aaacaggcgc aaaaattacc tgcacggata atatccgcga aggcgtaaag
      361 aacgcggacg tcctctacac cgatgtctgg gtttccatgg gagagcccga tgaagtgtgg
      421 gcaagcagaa caaaactgtt aaaaccctat caggtcaatc aggaaatgat tgaattaacc
      481 ggaaacgata aggtgatctt tgaacattgc ctgccatcgt tccacgacca gaaaacgaaa
      541 atcggaaaag aaatctacga aaagtatggg ctcaaagaga tggaagtcac cgacgaggta
      601 tttgaaagca aacattccgt ggttttcgac gaagccgaaa accggatgca caccatcaaa
      661 gcagtgatgg tcgctactct gggggagtaa tcaaaaaaac aaaataggga ggcatagagt
      721 aatatggaac gcacaaatga tgaaggcggc agcaagaaac tcggcctgtt tttgttggtt
      781 atgctcattg ttggttccat catcggttcg ggtattttca acagccccac cgatctgatt
      841 aacaaagcga atcctctggc taccatcatc gcctggctgg ttggcggctt tggcgttttg
      901 atgctggtgc tgatgttcca gatgctgtcg acgaaaaagc cggagctcaa aggcggaatc
      961 tattcttatg ccaaagcggg atttggcgat tacgttgggt ttaactccgc ctggggctac
     1021 tggctgagcg cttttctggg caatgtggct tttattacta tggtctttaa aactctcaac
     1081 agcatgcttg gggacaaaca tcagcttagt tctttcgcga cttttctgct gggttccatc
     1141 cttctctggg tgtatcatct gattatcacc cgcggaatta aacaggcaac cgtgctcaac
     1201 gcggtgatta ccattgccaa atttgttcct cttatattgg tcatcgtttt tgggatcgcc
     1261 gtattcagct ccggaacctt taccgttcag aactggatga gtaatctcgc ctctaccggt
     1321 gaaagcacaa gtctcggtgc gcagattaac aatgccatgg gaaccattct ttggtgtttt
     1381 ttcggtgtgg aagcggctgt tgtcatgtcc aacagagcaa agagctcaaa gcttgttgga
     1441 aaatcgaccg tgatcagttt tctgattgtg ctcgttctct acatgctgat ttcgatcatt
     1501 tcaatggggg ttgtgccagc aaaacagctg gcaggttccg caacgcctct ggcggatgtg
     1561 ctgagcaaaa ccttccttgg aagcgccggc tccgttatcg tacatcttgg tattttaatt
     1621 tcaattctcg gcgcgctggt cagctggctg atgctgactg ctgaagttcc ttacattgcg
     1681 tcaaaagacg gcgtaatgcc caaatggttc tccaaagaaa atcatgcggg agtaccgatc
     1741 aattccctga ttttctccaa tggaattacg cagctgtttt tgctgtttct gctgattcct
     1801 cagcttcaga ccgcttacag cgcagcctat acccttgcca cgaccgctat tctggttcct
     1861 tacttattct ccgctttgta tgcgctgaaa ataaccatca ccgaaaagct aaacgccgga
     1921 tgtctcgcta tttcggttct ggcaactctt tattccgtct atgtgattta cgccgtgggc
     1981 ctgaaatatc ttgcggcggc agtgatcctt tacgcaatcg gcggaatccc gttttatttt
     2041 gcgagaagag aaaaatcaca gagtctttca agaaatgagc ggattgcact tggaatcata
     2101 gttatgatcg cccttgctat gattgttatg attgccacag gagtcattca actttaaaaa
     2161 tacgtcggat tatgaaatgg tcatggacgg agacatagga atgattacat ttcagaaagc
     2221 ggtcgcttat ctgatagaca ggaaaaagtc atcgcagaac ccgcgggagc cgcttccgta
     2281 gccgctgtga tggaaaacaa agaattgtat cgccgtaaaa acgtggcggc catcgtttcc
     2341 ggagggaatt tagacgaaaa actcatgtga aaaatattat cggtatacag ctgataaacc
     2401 gataggactg gactgtttgc ctttcaggca actatttgtt aaggggagtc actaatggca
     2461 agaatcgtaa tcgccctcgg tggaaacgcg cttcagtcag acccaaaaga tatttccgcc
     2521 aaggcccagc agcaaacgat agaatcaatt tcgggcgtac tgaccgatct gattgaaaag
     2581 ggccatgaaa ttctgttcgt tcacggcaac ggcccgcagg taggccagat catgtcctct
     2641 tatgaaacgt ccgcaacggt gaatcctttg cttcccgtga tgccgtttcc ggagtgcggg
     2701 gcaatgagtc agggatacat cggctatcat cttcagcaga ctttaaaaaa cgcgatggtg
     2761 agacggggta tcaaaaagga aattgtaacg attattacgc aaacagtggt tgataaaaac
     2821 gatcctgctt ttcagcggcc ttccaaaccg gttggttcct tttataccaa ggaacaggcc
     2881 aaagagttgg aaaccacaag gggctacgtc atgaaggaag atgcaaaccg gggctatcga
     2941 agagttgttc cgtcgcccaa acctcttgaa ttcgtggagg aaccaattat cagcgcacta
     3001 ttggatgccg gtcatctggt cattagcgcc ggaggtggcg gagtgcccgt gattgatcgc
     3061 ggaaacggcg aacttgaagg cgtgccggcc gtaattgaca aagattttgc cgcatgcaaa
     3121 ctggcggaag acattcatgc cgacgcgcta atcattttaa ccgcggtcga caacgtcgca
     3181 gtcaacttcg gcagaccgga tcagaaaaat ctgacgaatc ttaccgtcag cgaggcggaa
     3241 gccttcatcg cgcagggaca attcgctccc ggttccatgc ttcccaaagt tgaggctgcc
     3301 atcagtttcg taaaatccga gccgaaccgc aaagcagtca tcacttcgct ttgccaggct
     3361 ctggaagcaa tcggcgggaa ggccggaacc gttgttacca tttaagaaaa agcagaccat
     3421 aatttaaatt atggtctgct ttttaaaaac aaattaattt taatgattaa ccgcctttaa
     3481 ggctccctta cagaaaactt cacattgact ccccgaccgt gaaggcctgt ttcgataagc
     3541 gcaatactca caattcgacg gcaagcgtct gtctgtcgac ctgattggat ctgaaatcct
     3601 ttcattatcg caatagtcat attttttcat tactacagac ctccttcttg aatagatccc
     3661 ctgaacaggg ccgggataaa gcggatcgaa aaggcatacg cccggctcag caggaataat
     3721 gtatatgtta tgtataagaa taacatatac cggtatatat gtcaatataa ttccactatc
     3781 aatgtatttt tatacatgat ctcatttttc agctcggctg agcccgctaa aaatccccct
     3841 acaggaacag gttgtttccg tgggggggat ttgttagttc ggttctgaca caaacgagga
     3901 ttacccccgc cttccgggct gacgatcttt aaaccgatga gacgcgtaac cggacggagc
     3961 cctccggggt tcttcccggt cgtgaggttt tgcgtcgaca aacattcctt taattttctg
     4021 gcctttcagg gaatcaatta cttcctgcct ttgcgcgccg gggatctcga caattgttga
     4081 attatccaga acctgaatct tcccgatgtc gctgccggaa agacttgtct tttccgcaat
     4141 ggcgccgacg atatgattcg gagaaatatg ctgcattttc ccaatgttca ggataattct
     4201 ggatgatcca tctttcggag atggacgttc cctcttggaa ggagaaaact ctctggcaaa
     4261 atcaatcacc gctttgcggc gctttctgtc tttttcggga tgcgaaacct ctgcggaatc
     4321 cggtttttgt ctctgcacaa ttccgccggt acggacctcc tcccgaatga tcgaggactt
     4381 tgtaactctc tcgatatttc taagctgcag gatctgtttt ctgccgctga taatcgtata
     4441 ggctttgccg gattttccgg cccgtccggt tcttccgatc cggtggatat agtcttcaac
     4501 gtcggccgga atgtcaaaat taacaaccat ttcaacatcc tgcgcgtcta ttccgcgcgc
     4561 cgcgacatcg gtcgcgacaa gaatcggaac ccggccggct ttaaattttt ccataacgcg
     4621 ggttcttaca ttctgggtga tccgcccatg aatccccact gcttcaaagc cttggcgtac
     4681 cagaaagatc accagaccgt caaccatttt ctgcgtattg cagaatacaa tcgccctgcc
     4741 cgggttttca cttttcaaaa ggccggcgag ggcttcctgc ttgccggacg ccgacgtctc
     4801 aaagtaatac tgactgatcg cctcaagagt cacctgattt ctgttgattt caaccagctc
     4861 agggtgtttc tgatacgttt ttgtgatggc cataatctcc ggcggcatgg tcgcacagaa
     4921 aaagacggtc tgccgctctt cgggcgcctg gctgagaatg gtctcaatat cctcccggaa
     4981 ccccatattg agcatttcat ccgcttcatc aagaataacc attttcaggt ggttgagttt
     5041 gagcgtcttg cggcgcaaat gatccatgat tcttcccggt gttccgatta cgatattaac
     5101 gccctttttc agctctttga tttgccggtc aatcggctgc ccgccataaa cgacggcggc
     5161 ctttataccc gatttgaact gataaagctt tttaaactcg ccccaggact ggatagccag
     5221 ttcccgggtc gggcagacta caagaacctg cacctgaaat ttatgatcca cgtcgatcat
     5281 ttcaaccgca gggatggcga aagatgccgt ttttcccgta cctgtctgtg accgcccgat
     5341 aacatcctta ccctcgagaa tcaaaggtat ggtttttgcc tgtatttcgg tggcgttttc
     5401 gaatcccatc gcatgcgttg cacgctggat ttccgatgta agattcagac tgctgaatgg
     5461 aattggtttc attatattcc ccttttcatt attatctgtt tctcgtttat cctttgctaa
     5521 aaatgcgccg aacatgtaca aacgcatatg aaaaaacgag gatgccccat cctcgctgaa
     5581 gtctaagagt aaaaatttaa tctgtaacag cgtgaccgac tgcgcaatgg gagcttatca
     5641 agcggcaagt cacttttgat acgacagcaa ttacccggcc tcatgtaaaa aagaatgcaa
     5701 gatattaatt ataacatacc gtaaagaaat cgtcaaaaaa gcatttctga tccacgggtg
     5761 ttttaaccat ccgcatttta tccctgcgcg acggtttctg ttccccatgg cgaaaaggga
     5821 aacccgcgca tttttgcgcg ggtttctatc gagccagctt tgctatgatg cgggaaaaac
     5881 ctattccacg gcaataaccg ataccgggca gctttcctgc gcttccaccg cttttgcctc
     5941 ggcttcggcc ggaacttctt ccgcatggac ctctgcaaac ccgtcctccg ccattctgaa
     6001 aacctcggga cagacttccg gacaaagtcc gcaggaaatg catccgcttc tgtcaattgt
     6061 tgctttcatc aagatcttcc tttctttttt actgattcat gaacgagata agcttccgcg
     6121 tttgagaaga aaacttcacg gatacgcaca aaccatatgg ttaacattcc ctttagaact
     6181 ttgtttactc catcatcagc ttcttttcgc ccgtaatctt ctgaaatggc aaaaatcttc
     6241 tgttgcctga ttcaccacaa gaccgtactc ctcaccgttt tgtgtcaaca gccccggcct
     6301 cggacgggat cgtaagttta gagaactcct ttttgagttc ccgtgccgac cgcgcaatat
     6361 catccgccgc catcagggca gagacaaccg cgataccgtc gataccggtc tgatcaaaca
     6421 aaggaatggt atccggcccg ataccgccga tgacaacaat cggaatcgtg acggcagccc
     6481 gaatcacttt caacgattcc attgtcacga taccagcatg cgattttgtg cctgtggcaa
     6541 acattgctcc gacgcccaga taatccgcgc cgtcccgttc ggcccgaacg gcctgttccg
     6601 gtgtttcgac cgatacccca agtatttgat ccgttccgag caaggaccgg acgacgccgg
     6661 cgggcagatc gttctgtccg atatggacgc ctgccgcacc gactgccagc gcaatgtcga
     6721 cccggtcatt gatcaccagc ggcaccctcc cgcggtcggt aacgatttta atttcttccg
     6781 ccagccggta aaactccagt gaggacaccg ttttttcccg cagctgtatc agcgtgcagc
     6841 ctccctgaat cgcctgctcg accgcaaggc taagcgtagg cgtacgcatc agattccggt
     6901 cagtgaccag ataaagactg cagtctaaat caggcttcat ggatttttgc cctcccggca
     6961 agcgtttcag cgttaagcct gccgatctgg tcgataacgg aaagatgata gctgccgttt
     7021 ccaccggcgc cgatttcctg cgcgagctcg cccgccaggc tcatggaaag aacgcccccc
     7081 gccgccgcgg caaagggatc accgcccgcc ccgcaaaacg ccccgatcag tgaggtacac
     7141 atacagcccg ttcctgttac gccggaaagc agacggcttc cgttttcaat cagaacggtg
     7201 cgtttcccat cgcatatgac gtcggtcggg cccgtcaccg ccgtaacgca cccgaattgc
     7261 tccgccgctt tcattgccac ttcttttccc gcccggatgc cggtcagcag atcggcttcg
     7321 gaggcgtcga ccccctttgt ttcggcgcaa aaaccggcaa ccgaccggat ttccgagcta
     7381 ttccctctta aaacagtgat tttgagttct gtgagcagcc gttcggccac acggtttcga
     7441 tagcctgacg cgcccgcccc gaccgggtcg aatacaacgg gaatctgaag ctcattggct
     7501 ttcttccccg cggtcagcat cgactctgcc gtcctccgat tgagggtgcc gatgttcagt
     7561 accagcgcgg aggaaatggc gacgatatct tctacctctt cggcggcgtc ggccattacc
     7621 ggagacgccc cgatggcaag caccgcgttg gcgcagtcgt taaccgtaac ataattggta
     7681 atgttgtgga tcagcggctt tttacgcctg atttcctcca tgtttttgat gatttccgcc
     7741 aaaatgccga gcttcatttg ctttcgttcc tttccgcctt ttctcttttg ctttccggtt
     7801 ttaaatccat atcctgtcca ctgagaattc ggttcatcgt tctcaccgcg cacattttac
     7861 cgcacatgga acagctgtgt ttatcttcag ggggaacact ttcataatag gccctcgcct
     7921 tttccccgtc gagcgcaagg gaaaacattg cttcccaatc aagcgtacgt cttgcctcgc
     7981 caattttgta atcggcatcg caggctcccc ggacaccttt tgcgatatcg gcggcgtgag
     8041 ccgctatttt ggaggcgatc agcccttctt tcacatccga cagatcaggc agtctcagat
     8101 gttcggccgg agttacataa caaaggaaat ccgcgccgtt tgccgccgcg atggctcctc
     8161 cgatcgccga agtgatatgg tcatagccgg gcgcaatatc ggtgacaagc ggcccgagca
     8221 cataaaacgg cgcgccgtgg caaaggcgtt tttcaagcgt catattcgct gcgatctcat
     8281 tcatcgccat atggcccggt ccttccacga ttacctgaac gtccttcgcc cacgcgcgtt
     8341 ttgtgaggac tcccaattca gtcagctcgg cgatctgacc ggcgtcggtg gagtcatgga
     8401 tactgcccgg gcggagagcg tccccgagac tgacggtcac atcgtattca cacagaatct
     8461 caagcagctc atcaaagtgc tcgtaaaacg ggttttcgtt gccggtcagc ttcatccacg
     8521 cgtaaagcag ggaaccgccg cgggaaacaa tgttcatctg gcgcttttcc ctctcaaata
     8581 ctgccgccac ccggcggttt aagcctgcgt ggatcgtcat aaaatcgacg ccctcctcgg
     8641 catgggaacg cacaactctg agaaagtccg ccgccgtaat ctccgtcaga tccttttcca
     8701 gataaccgat ggcgtcatag accggaaccg ttcctatcat cgcgccggac ttctcaatca
     8761 gctttttgcg aaactccctg gtcttcccgt agttgctcag atccatgatt gcttccgcac
     8821 catagcggac cgcaagctcg accttttcca tctcggcccg ataatcggcg cagtcgccgg
     8881 aaattccgag gttgacgttg atttttgtcc taagcccccg tccaacgcca aagggatgca
     8941 gggaatgatg ccggatatta gccggaatca ctgtcgtccc cgccgcgaca agctccatca
     9001 gttcccgctc cgaaatgccc tcgtccttcg cgacctgagc gagctgcctt gtgacaattc
     9061 catttctggc cgccttcatc tgggtattgc tgttcattgt atttcgctcc tttcaatcca
     9121 tgctggtaaa gatcataaaa atggttggtt gggccccatc ctccgccgag aggcagcgca
     9181 tgccggatgg cggtggtcac atagtccttc gcgcgtccga ccgcctcccg aagggggagc
     9241 cccagcgcaa ggccggacgc aatggcggag gaataggtgc agccggttcc atgtgtgttt
     9301 ttggtaagaa tcctctcacc gcagaacaac gtaaactcca ccccgtcaaa aagaacatcc
     9361 gtcgggctcc cggcagaatg ccccccttta atcagaacgg ctttgggccc cataccggcg
     9421 atctgccggg cggcccgttt catctcgtcg agcgaaccga ttgtcttgcc ggttaaaacc
     9481 tcggcctccg gaatattggg cgtaagcagg gcggcaagcg gaaatatttt atccgtcaac
     9541 tctcccacgg cggatggctc catgagggga acaccgcttt tggaaatcat gacagggtcg
     9601 acgaccacgt ttcgcggccg gtattcccgg acctttcgcg ccacagcctt catacagtcc
     9661 gcctgcgaca gcatcccgat cttgacggca tcggcgccga tatcctccag aacggcatcc
     9721 agctgttttt caatcatggc ggctgaaata tcctgccggt ccatcacacg caaagtgttc
     9781 tccgccacca ccgagacaat cacgctcata ccgtaaacgc cgtgggcggc aaaagtttta
     9841 agatccgcct gaatccccgc gcctccgctg cagtcggagc cggcaatggt cagcgctgtt
     9901 ttcattcaca tcacccggcc tttttcaaat tgattttcac tctgtttcgc aagggacatt
     9961 ctgaaagctc ctgctgtctg tcataaaagc caaaacaaaa aaggcgcccg ccgtatacag
    10021 caagcaccct catccgacat tctatcggaa tcattcactt ccctacgaca gttttaactg
    10081 acaggttcaa agggtcaggt tttaccttct caactccgaa gagtcccccc gcaatgcagt
    10141 cttcagtttt tatccggcag aaccgggccc ggatcttgtt cgccataaaa tcgtatgggc
    10201 gcgcccggga atttttcttc tttacagaac aaaaaagcgc tctccctttc aggaaagcac
    10261 cgatcaaatc attcattgat ttgcttccct tcgccagtgt taactgacag gttcaaaggg
    10321 tcaggtttca ccttctcaac tccaaagagt ccccccgcaa acagaccgta tttttttcgc
    10381 cgcggcaatt ttaccacccg gcggtcttat tgtaacaccg ccgcgcacct ctgtcaattt
    10441 gtgtatcatt tttccgtatc ccttccggcg caccgaacat tgtattcagg cacggatcgt
    10501 gctaagataa aacagaaaag atcatattgg aggctcgccg tatgtctgac tggaacccct
    10561 ccctctatct gaaggtaaaa cctgaccctt tgaacctgtc agtta
//
LOCUS       DATALY010000185         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_302077,
            whole genome shotgun sequence.
ACCESSION   DATALY010000185 DATALY010000000
VERSION     DATALY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302077"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VHR42_05395"
     CDS             complement(<1..803)
                     /locus_tag="VHR42_05395"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX3026643.1"
                     /translation="MLISKCLGVNREGHLTIGECDTVSLAKDYGTPLYVMDEETIRTN
                     ARSYRDSINRYYNCKGKVLYASKAFCCKEMCRIAREEGLGLDIVSGGELFTALKAGFD
                     PKDIFFHGNNKTRQEIVYALECGVGRFVADNLSELEMLDSLAGELGKKAHILFRIKPG
                     VDAHTHDFIRTGQIDSKFGFALETGEAEEAVARASGMQHLVLRGIHCHIGSQIFEIEP
                     FELAADIMIGFMAKIKQRLGIEFDELNLGGGFGIKYVASHDPIEYDRYL"
     gene            complement(934..1521)
                     /gene="yihA"
                     /locus_tag="VHR42_05400"
     CDS             complement(934..1521)
                     /gene="yihA"
                     /locus_tag="VHR42_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX3026644.1"
                     /translation="MNLSETVLETTAYNIGQLPFSDLPEIVFSGRSNVGKSSMINKLI
                     NRKSLARVSSAPGKTASVNFYRCGEIRLVDLPGYGFAKVSKEEKQKWSELVEGFFAAD
                     RRISLMIQLLDMRHKPTEDDLMMMDYIIRSGHSLLIVLTKSDKLNKTEYALRKQSIVT
                     ELGEIAQVAQEIIPFSSKQGEGTDEIKKAIFASLK"
     gene            complement(1540..>3355)
                     /gene="lon"
                     /locus_tag="VHR42_05405"
     CDS             complement(1540..>3355)
                     /gene="lon"
                     /locus_tag="VHR42_05405"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX3026645.1"
                     /translation="EREIGILNIEQEISEKIHDQVDKNQREYYIREQIKVLSGELGEA
                     DNPQDEAIAYHKKIDGLKLKEEAAKRLHAEADRLMKMPSGSHEATVIRNWLDTCLSLP
                     WNKSTKDKIDLGKARKVLDEDHYGLEKVKDRMIEFLAVKKLTSKLNGQIVCLVGPPGV
                     GKTSIARSIAKAMGRHYIRVSLGGVRDEADIRGHRKTYIGAMPGRIIHALELAGTNNP
                     LMLLDEVDKMSADFRGDPSSALLEVLDSEQNNSFRDHYVELPFDLSNVMFLTTANDLD
                     SIPEPLLDRMEIITLSTYTREEKYHIALEHLLQKQLKKHGLNKRMLKIKEDSIYALID
                     FYTRESGVRNLERQIASICRKAAKQIAADGKHQVVVAAEDLEQLLGPKKYKQEPFSRT
                     DEVGVVTGLAWTSVGGETMPIEVCVMEGSGKLELTGSLGDVMKESAKAAVSFIRSRAG
                     KLMVDADFYKEKDIHIHVPEGAIPKDGPSAGVTMATALVSALSGRKVRHDVAMTGEIT
                     LSGRVLPIGGLREKTMAAYRDGIKTVIIPEENRPDLADVDSIVKEHIRFIPVSRVESV
                     FETAIINPDEKKNDQVVGKATIINTIPVVDAQLPVIRQ"
BASE COUNT          791 a          933 c          725 g          906 t
ORIGIN      
        1 tcaagatagc ggtcatattc gatcgggtcg tgtgaagcga catatttgat gccaaaccct
       61 ccgccgagat tgagttcatc aaattcaatc cccaaacgct gtttgatctt cgccataaaa
      121 ccgatcatga tatccgccgc aagctcaaac ggctcaattt caaagatctg cgatccaata
      181 tggcagtgga ttcctcttaa aacaagatgc tgcattccgg atgcccgggc taccgcttcc
      241 tccgcctcac ccgtctcaag tgcgaaacca aacttggagt cgatctgccc ggttcggatg
      301 aagtcatggg tatgagcatc cacgcccggt ttgatccgga ataaaatatg ggctttcttc
      361 cccagttccc ccgcaagact gtcgagcatc tccaactcgg aaagattatc cgcgacaaag
      421 cggcccacgc cgcattccag cgcatagacg atttcctgtc tggttttgtt attgccgtga
      481 aaaaagatgt ctttcgggtc gaaaccggcc tttaaagccg taaagagctc tcctccggaa
      541 acaatatcca gccccagccc ttcttctctc gcaatccggc acatttcctt acagcaaaac
      601 gctttactgg cgtaaagtac tttaccttta cagttataat accgatttat cgaatcgcga
      661 tacgatcgtg cattcgtccg gatcgtttct tcgtccatta cataaagagg cgttccgtaa
      721 tcctttgcaa gactgacggt atcgcattca ccaatggtca ggtggccttc cctgtttacc
      781 cccaggcact tggatattaa cacaattgca actctcctga caataaccgc gaccgcgcgg
      841 aaatatttct tcgaaacggg ctcgataagg gcccgaccgt ataccacaaa aaagcctgca
      901 tccgaagaaa caggcttttt cggatcctaa atcttatttc agacttgcga aaatagcttt
      961 cttgatctca tccgttccct caccctgttt cgatgaaaac ggtataattt cctgcgctac
     1021 ctgagctatc tcgccaagct cggtgacaat ggactgtttt ctaagcgcat attcggtctt
     1081 attcagttta tcgcttttgg ttaaaacaat taacagacta tgaccgctgc ggataatata
     1141 atccatcatc atcaggtcat cctccgttgg cttatgacgc atgtcaagca gctgaatcat
     1201 cagggatatt ctgcgatcgg cggcaaaaaa cccttccacc agctccgacc acttctgttt
     1261 ttcctctttc gaaacctttg caaatccata gccgggaaga tcaaccagcc ggatttcgcc
     1321 gcagcggtag aaattcaccg aagccgtttt ccccggcgcg gaactgactc tggcaaggct
     1381 ttttcgattg atcagcttat taatcatcga agacttgccc acatttgacc tgccggaaaa
     1441 tacaatctcc ggcagatcgg aaaatgggag ctgcccaata ttatacgccg ttgtttccag
     1501 aactgtttcg gaaagattca atacaagcac ccctaaactt tattgtctga tgaccggaag
     1561 ctgcgcgtcc accaccggaa tcgtgttgat aatcgtagcc ttgccgacga cctgatcatt
     1621 ttttttctca tccggattga tgatcgccgt ctcaaacacc gactccacac ggctcacggg
     1681 tatgaaacgt atgtgctcct tcacaatcga atccacatcg gcgagatcag gacggttctc
     1741 ctcgggaata atgaccgttt taatgccgtc gcgataggcc gccatcgttt tctcacgaag
     1801 gcctccaatc ggcagaaccc tgccactgag tgtaatttca cctgtcatgg caacatcgtg
     1861 gcgaaccttc ctgcccgaca gggccgacac cagcgccgta gccatcgtga cacctgccga
     1921 agggccgtct tttggtatgg ctccctctgg cacatggatg tgaatgtctt tctctttata
     1981 aaaatcggca tccaccataa gttttccggc ccggcttctg ataaagctga cagccgcttt
     2041 cgccgactcc ttcattacat cgcccagaga cccggtcagc tccagttttc ccgagccttc
     2101 catgacgcaa acttcaatcg gcatagtctc cccgcctacc gatgtccacg cgaggccggt
     2161 cactactccc acctcatccg tgcgggaaaa gggctcctgt ttatattttt tcgggccgag
     2221 aagctgttca agatcttccg cggcaaccac aacctgatgt ttcccgtccg cggctatctg
     2281 cttggccgcc ttacggcata tcgacgcaat ctgcctttcg agattacgga cgcccgactc
     2341 ccgggtgtag aaatcaatca gagcgtaaat ggagtcctct tttattttaa gcattctctt
     2401 attcaaacca tgttttttca gctgtttttg caggaggtgt tcgagcgcaa tgtggtattt
     2461 ttcttccctt gtgtaggtag agagcgtgat gatctccatc ctgtccaaaa gaggctccgg
     2521 aatactatcc agatcatttg ccgtcgtaag gaacatgaca ttgcttaaat caaaaggcag
     2581 ctctacatag tggtcgcgga aagaattatt ctgctcgctg tcaagcacct ccagcagcgc
     2641 cgaggaagga tcgcccctga aatcagccga cattttatcg acttcatcca gaagcatgag
     2701 cggattattg gttcccgcca gctccaaagc atgaataatt ctgccgggca tcgcccctat
     2761 gtaggttttc cggtggccgc ggatatcagc ctcatccctg acgccgccga gcgacacacg
     2821 gatatagtgt ctgcccattg ccttggcaat tgagcgggca atggatgttt ttcctacgcc
     2881 gggagggccc acgaggcaga cgatctggcc gtttaacttt gaagtcagtt ttttaaccgc
     2941 cagaaactcg atcattctgt cctttacttt ttcaaggccg taatggtctt catccaacac
     3001 ttttctggct ttgccgagat caattttgtc tttggtcgat ttattccacg gcagagaaag
     3061 gcaggtatcg agccaattcc gaatcaccgt ggcttcatga gaaccggatg gcatcttcat
     3121 tagacggtca gcttcggcat gaaggcgttt tgcggcttct tcctttaact tcaggccgtc
     3181 gattttttta tgataggcga ttgcttcatc ctgagggtta tcggcctccc caagttcacc
     3241 ggaaagaacc ttgatctgtt cccgaatata atattctctc tggtttttat cgacctggtc
     3301 gtgaatcttt tcactgatct cctgttcgat attcaaaatg ccgatttcgc gttcc
//
LOCUS       DATALY010000186         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_385898,
            whole genome shotgun sequence.
ACCESSION   DATALY010000186 DATALY010000000
VERSION     DATALY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385898"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VHR42_05410"
     CDS             complement(<1..776)
                     /locus_tag="VHR42_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026646.1"
                     /translation="METGHRLAPHVIEGRKDFRSLKFLDRLRPVFESFGVDYPVMRKI
                     LRIKLMMDSRRVPTVMSNRQSGNEGSRSNRSSLLLDGFLGLFIGLMVVMPMPLFIKMN
                     IVLGMLLFMIMTTMISDFSSVLLDLRDKNILLTLPVNARTLNAAKLLHIVIYLTEITA
                     ALAAASLAAGLIHCGVWFFLLLLFELILICGFVLLFTSILYFIILRFFSGEKLKDMIT
                     YFQILLSVCMTLGYQLVGRLFDFSGGSGHFSPQWWNFLLPS"
     gene            complement(782..1567)
                     /locus_tag="VHR42_05415"
     CDS             complement(782..1567)
                     /locus_tag="VHR42_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026647.1"
                     /translation="MNPTGEPVISIKDLRMSYGSKEVLKGIDLEVYRGQIIGYIGPNG
                     AGKSTRVKILLGILEGYQGEVKVFGENLSSGSIEYKKRIGYVPETADVYESLTAQEYL
                     TFIGELYGIDYATADSKANRLMSLFGIGEGYTARISSFSKGMKQKVLIIASLLHNPDI
                     LFFDEPLSGLDANSVMVFKEVMSELASQGKTIFYSSHIMEVVEKISDRIIILSGGHIM
                     ADGTFEELKEQCREGSLEEIFTQLTGFDRQRDIAGEFISILKE"
     gene            complement(1646..2590)
                     /locus_tag="VHR42_05420"
     CDS             complement(1646..2590)
                     /locus_tag="VHR42_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3026648.1"
                     /translation="MIWLLIAACITAAVFGIRLFQIKRQLNHIKVQLDERTAGKTEKK
                     ITVSLIDGNLIELAAAINRSLITQKELRTEVWRKDRQLKDSIANLSHDLRTPLTSILG
                     YLQLFRDPDCPSEKRNDYLRIIDDKAHVLKTLINDLYELSVLDTRELNKEDLDLNLLL
                     SDVLAGQYELFHKSGIVLNVKLPDSPVWTSGDRVACIRILQNLLNNTIRYAQKSAEIV
                     LEKKESHAFLSICNSSPNLTQEDVEHLFDRFYTADKSRTGGGTGLGLSIVKDLLLQMG
                     GTVEATYRNNNLCLTIQLPVLTETQIGKGANPDSGQPC"
BASE COUNT          675 a          649 c          626 g          664 t
ORIGIN      
        1 gagggcagca gaaaattcca ccactgtggg gagaaatgcc cgctcccgcc ggaaaaatca
       61 aataaccgcc ctactaattg gtagcccaga gtcatgcaga cggaaaggag aatttgaaaa
      121 taagtgatca tatccttgag cttttctccg ctgaaaaaac gcaggatgat aaaatagagg
      181 atcgaagtga acaaaagcac aaatccgcag atcaggatca gctcaaacag aagaagcagg
      241 aagaaccata ctccgcagtg gatcagccca gccgcaagcg aagccgcggc cagcgcggcc
      301 gttatttcgg tcagataaat cacaatatgc aggagctttg ccgcattgag cgtacgtgcg
      361 tttaccggta gggtgagcag gatgttctta tctcttaaat ccagcagcac ggaagaaaaa
      421 tccgagatca ttgtggtcat gatcataaac agcagcatac ccaacacgat gttcattttt
      481 ataaacagcg gcatgggcat aaccaccatt aacccgataa acaggccgag aaagccgtcg
      541 agaagcaggg aagaccggtt ggagcgacta ccctcgttac cgctctgccg gttgctcatg
      601 acagtaggca ctctgcggct gtccatcatc agcttgattc ggagaatttt gcgcatgacg
      661 gggtaatcga ccccgaaaga ttcgaaaacc ggccgcagtc ggtcgagaaa cttaagagac
      721 cgaaaatcct ttcttccttc tataacatgg ggcgcaagcc tatgacccgt ttccatcccg
      781 ttcactcttt caatatggag ataaactcgc cggcgatatc tctctgtcgg tcaaaaccgg
      841 tgagctgggt gaaaatctct tcaagcgagc cttcacgaca ctgttccttg agttcttcaa
      901 aggtgccgtc tgccatgata tgaccgccgc tcagaatgat gatccggtcg ctgatttttt
      961 cgaccacctc cataatatgg gaggagtaaa aaatcgtttt gccctgcgac gcaagctccg
     1021 acattacctc tttaaacacc atcacgctgt tggcgtcaag tccgctcaga ggttcatcaa
     1081 agaagagaat atccgggttg tgcaaaaggc tcgcgatgat aagaaccttt tgcttcatgc
     1141 cctttgaaaa ggaagaaatt cgcgcggtgt aaccttcgcc gatgccgaat aaagacatca
     1201 gacgatttgc tttgctgtcg gcggttgcgt aatcaattcc gtatagctcg ccgatgaagg
     1261 tgaggtattc ctgtgcggtg aggctctcgt atacgtcggc tgtttcgggc acgtaaccga
     1321 ttctcttctt gtattcgatg ctgccggagg acagattttc cccgaagacc ttcacttcac
     1381 cctgatatcc ctcaagaatt ccaagcagaa ttttgaccct cgtgcttttg cccgcgccgt
     1441 tcggcccgat gtaaccgatg atttgcccac gataaacttc gaggtcaatc cctttcagta
     1501 cttctttgct gccgtagctc attctcaggt ccttgatgga aatcaccggt tcgcccgtcg
     1561 gattcattac atttcactcc cgctttcaaa taatttccgg cctttagaaa aggcttacag
     1621 cattttaaag tgaataaagg ctgtactaac agggctggcc cgaatcggga tttgcgcctt
     1681 ttccaatctg tgtttctgtt aataccggaa gctgaattgt caggcaaaga ttgttgtttc
     1741 tgtaggtggc ttcaacggtt cctcccattt ggagcagaag gtcttttaca atcgaaaggc
     1801 ctaagcccgt gccgcctccg gttctggatt tatcagccgt atagaagcgg tcaaacagat
     1861 gctccacatc ttcctgcgtc agattcggcg aagagttgca gatcgacaaa aatgcgtgag
     1921 attctttttt ttccagaaca atctcagcac tcttttgtgc atatcggatc gtgttattca
     1981 gtaaattctg caggatgcgg atacatgcga cgcggtcgcc ggaagtccag acgggggaat
     2041 cggggagttt aacattcaga acgattccgg atttatgaaa cagttcatac tgcccggcca
     2101 acacatcgga gaggagaaga ttcaagtcca aatcttcttt gttcaactcc cgggtatcca
     2161 atacggacaa ttcataaaga tcgttaatca aggttttcaa cacatgcgct ttatcgtcaa
     2221 taatccggag ataatcgttc ctcttttctg aaggacaatc cgggtcgcgg aaaagctgca
     2281 gatatcctaa aatcgaggta agtggagtgc gaagatcgtg gctgaggttt gcaatggaat
     2341 ctttcagctg ccggtctttc cgccacacct ccgtgcggag ctccttctgc gttattaagc
     2401 tgcggtttat ggcggcggca agttcgatta aattgccgtc gatcagcgaa accgtaattt
     2461 ttttctcggt tttccccgct gtgcgttcat cgagctgaac cttgatgtgg ttcagttgcc
     2521 gcttaatctg gaaaaggcgg atgccgaata cggctgccgt gatgcatgcg gcaatcaaaa
     2581 gccatatcat taagcgccgc cttctttccc agac
//
LOCUS       DATALY010000187        16324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_127477,
            whole genome shotgun sequence.
ACCESSION   DATALY010000187 DATALY010000000
VERSION     DATALY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127477"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1052)
                     /locus_tag="VHR42_05425"
     CDS             complement(150..1052)
                     /locus_tag="VHR42_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX3026649.1"
                     /translation="MTHNSINKISQKISPEKAKEYFWDFIFILAGSIIYSASVAVFTA
                     PNNIAPGGITGIATILNYLIHTPIGLMYLILNIPLLVLSFKLISREFIVKTLLCTVLT
                     SVFVDGMALFVPTYTHSMLLAALYGGILSGVGLGLVFLRGATSGGSDVISRLMRLKFK
                     HVPMGKMMMVIDSLIVILSTFVFKSVDSGLYAFITIFTCSKVIDAMIYGSETGQMMLV
                     ISEKNEMIANKIMDELSRGVTMLKGRGFYTGHEKDVLMCTVRRPETAKLRSIVRNADP
                     NAFIVSCEASEVIGEGFKPITKED"
     gene            complement(1201..1620)
                     /locus_tag="VHR42_05430"
     CDS             complement(1201..1620)
                     /locus_tag="VHR42_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEX3026650.1"
                     /translation="MSDNSVMDQQKPATDPYRLFSPITLAFLGDAVFELKVRAHLIRS
                     GNIVAKKLHKKCVDYVRAAAQAESVRGIWSMLTEEEQEILRWGRNSNSAHIPKNADPI
                     EYRYATGLEALFGYLFLHEKHERIDEIFDAVVKSKNS"
     gene            complement(1610..2878)
                     /gene="obgE"
                     /locus_tag="VHR42_05435"
     CDS             complement(1610..2878)
                     /gene="obgE"
                     /locus_tag="VHR42_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX3026651.1"
                     /translation="MFVDKAKITIRAGNGGNGAVSFRREKYVAAGGPDGGDGGKGGDV
                     IFIVDDNYSTLADFRFKKKYEAPNGENGKQKKCFGKAGANLEIKVPRGTLIKDAKTGL
                     IVADLSSDEPYVAAKGGKGGWGNCHFATPTRQIPRFAKSGIPGEEKEIVLELKLLADV
                     GLLGFPNVGKSTLLSIVSEAKPNIANYHFTTITPVLGVVYHQEKSFVMADIPGLIEGA
                     SEGVGLGHEFLRHVDRCRLLIHIVDVSGSEGRDPVSDFEDINGELAKYSEELSKRPQI
                     VAGNKCDIAEQEQISHFRDYVESKGLVFFPISAATRSGVKELMDFTLRRLSELPPIIR
                     YEAQPLPQDDFSAQKEREIQITNHEGVYFVESEWLIQVIGSVNLDDYESLQYFHRVLK
                     NSGVIEKLEKAGIQQGDTVSIYDVEFEYVR"
     gene            3193..4485
                     /locus_tag="VHR42_05440"
     CDS             3193..4485
                     /locus_tag="VHR42_05440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX3026652.1"
                     /translation="MTGGFLTELERKTLSDNINFTAIRDGRFKNNQISVNFLMPLRKE
                     TAALHALIPLVLKRGCRPYPNMTLLNRRLKELYGARLDGGVGKRGEMQVISLYCAALD
                     DTYALEGETLLRECAGLLKSVLFDPIVTDGALSAEDVEVEKKNLADLIESQLNDKRHY
                     ASLKAKEAMCAGEAYGVSEYGTAAQALAITPAALYEAYQTMLKTAGVEIILVGPADKT
                     ECESLFAEAFAAVSRGKLLRCVTDVIREPGPVRTVEERLAVNQAKLVMGFRAGTAAPE
                     SEADTMQLASAILGGTPHSKLFENVREKLGLCYYCFSYFERIKGLVFVEAGIEEQNYQ
                     KAKDEILNQLELLKKGEFSDDELSSAKLSLENSYRELNDTLYDISSWYLSQAVTGQIR
                     TPDQTARALAGIDRGQIAPTAAKIKLDTVYLLAGERKD"
     gene            4489..5757
                     /locus_tag="VHR42_05445"
     CDS             4489..5757
                     /locus_tag="VHR42_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3026653.1"
                     /translation="MDLELIESKRLHEKIYYTRHPSGLDLFVSPKKGYSSQYAVFGTK
                     YGSIDNCFEFGGETVRVPEGIAHFLEHKLFESEDGNAFSRYAKTGANANAYTSFDRTC
                     YLFSATSNFYESFEILLDFVQNPYFTEQTVQKEQGIIGQEIKMYEDDPGWRVFFNLLS
                     ALYHTHPVKIDIAGTAGSIAQITSDLLYKCYRAFYNLNNMVLCVAGDVDPERILKTAD
                     RLLKTPAPSKVRSIFDPEPDTIVKQRVEQKLSVSVPLFCFGFKGSPAEGAEAARAEAE
                     SSILLEILGGDASGFYRRLYDKGLINSSFGLQYFSGRSFASTVVSGESREPKLVMEEF
                     LKEVKRLKSEGIPSEEFEMAKKAYYGRLASHFDSVDNVANGIIGSHFTGMGPFEIVEA
                     AARVTPENVAKRLANTFSEEKAALSVINPL"
     gene            5788..6738
                     /gene="lgt"
                     /locus_tag="VHR42_05450"
     CDS             5788..6738
                     /gene="lgt"
                     /locus_tag="VHR42_05450"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX3026654.1"
                     /translation="MESPITFPGLHLTFNIQRQALSFGKFGIYWYGIIIATGLVIAIL
                     YGLKRSKSFGITQDDLIDVIVFSIIAAIIGARLYFVLFYIDPSSGTNPYFSDPASIFN
                     VRSGGLAIYGGIIGAFVCATIVCKIKKISTGAVFDIAALGFLIGQSIGRWGNFINREA
                     YGAATSLPWRMVVDSTGTGYHPCFLYESLWCILGFIILHIYSKRRKFNGEIFLMYLMW
                     YSFGRFFIEGLRTDSLMLGNLRISQLVAGLLFVAAGIFIYLKRQKSNGLTDDQGEYKR
                     VYEDAAEAVRQVSDNSDETQSNDLPEDMKNDSRDDGGEEK"
     gene            6738..7595
                     /gene="folD"
                     /locus_tag="VHR42_05455"
     CDS             6738..7595
                     /gene="folD"
                     /locus_tag="VHR42_05455"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX3026655.1"
                     /translation="MAAVIINGKEVSAKVKNQITESVRELREQHGVTPGLAVIIVGGN
                     PASRVYVNNKEKDCAETGIHSEEYALDESTSQRDLLQLVDNLNKNPAINGILVQLPLP
                     SHLNEEEVINAISPEKDVDAFHPANVGRIMIGDFDFLPCTPSGIMDMLDDSGIEIAGK
                     ECVVVGRSNIVGKPMAMLLLHRNGTVTVCHSRTKNLEEVTRRADILVVAVGKAHFITA
                     GMVKPGAVVIDVGMNRLDNGKLTGDVDFDAVAPIASYITPVPGGVGPMTRATLLKNTL
                     RAAKLQNGI"
     gene            7710..8021
                     /gene="rplU"
                     /locus_tag="VHR42_05460"
     CDS             7710..8021
                     /gene="rplU"
                     /locus_tag="VHR42_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX3026656.1"
                     /translation="MYAVIETGGKQYRVQEGDTIFIEKLGSDEGMEVSFDKVVALGKD
                     EGLEVGAPYVDGVKVSAKVLKNGKGKKIKVFKFKAKKGYRNRKGHRQPYTKVQIEKIV
                     A"
     gene            8021..8359
                     /locus_tag="VHR42_05465"
     CDS             8021..8359
                     /locus_tag="VHR42_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEX3026657.1"
                     /translation="MITARFFLKNGAVAGFEVSGHAGMDAQGHDVACASVTSAVQMAA
                     NTVTEIIGAKAGIQVDEALIKMELADTNGAESEKAVQAVLKGMRLHLKLLTQEFQEFI
                     RVEDMEVQSK"
     gene            8356..8640
                     /gene="rpmA"
                     /locus_tag="VHR42_05470"
     CDS             8356..8640
                     /gene="rpmA"
                     /locus_tag="VHR42_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX3026658.1"
                     /translation="MIKLNMQFFAHKKGVGSTKNGRDSESKRLGVKRADGQFVLAGNI
                     LVRQRGTKIHPGANVGRGSDDTLFALSSGKVRFERVGRDRKKVSVYVPEQ"
     gene            complement(8719..9141)
                     /locus_tag="VHR42_05475"
     CDS             complement(8719..9141)
                     /locus_tag="VHR42_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3026659.1"
                     /translation="MNIAFFLTPKKDVVFQLFNSTMRQAMERMEYHRYSAIPILSEDG
                     QYVGTLTEGDLLWKLKNTPRLNFKNTSKILISDVQLHVSNKPVHINCDMEDLLTTAIN
                     QNFIPVVDDNNKFIGIIKRSVIINYCFEKLFAKVVKEA"
     gene            complement(9255..10220)
                     /locus_tag="VHR42_05480"
     CDS             complement(9255..10220)
                     /locus_tag="VHR42_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HEX3026660.1"
                     /translation="MNLIGMPLKPEAYQGSGQKAPYFEGWYYRVQTAGGETVALIPGI
                     CKTKNGAHAFVQLIASFLERTFYFDYPARKFVPSGGELLFCVGDNLFSNDRMELDIRR
                     EIAVRGTVEFRNPTEYSAGFWGRDIMGPFSLLPYLECRHGVVCVKSSLAGSLNISGRT
                     MDFDGGTGYIEKDWGRFFPRSYVWMESARFAHSDASFMLSYADVPVFGKSIRGLIAFL
                     YFRGKFYRFATYSAASVTRFFQKNGEIFLRAESPFFRIDVKAYSGQGGELAAPAEDGM
                     AGRIRECSNGTLEVRLTDRHDRTIFQDTAANAGIEIVDPDDRLTR"
     gene            complement(10302..10718)
                     /locus_tag="VHR42_05485"
     CDS             complement(10302..10718)
                     /locus_tag="VHR42_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HEX3026661.1"
                     /translation="MLLAVVDGQGGGIGKLIVEKTRAEFGGQIEILALGTNALATSLM
                     LKAGADEGASGENAVVFNASKADVIMGSVGIIASNSMRGELTPLMAKAIAESPAKKIL
                     IPLNRCGLYVVDAVSKPLSAYADDAVLLLKNLCCSK"
     gene            complement(10748..11524)
                     /locus_tag="VHR42_05490"
     CDS             complement(10748..11524)
                     /locus_tag="VHR42_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026662.1"
                     /translation="MKNKSNAAVFELNHVEFAYPGQNMVLKDINLVIGPGEGVSILGA
                     NGCGKSTLLKMLTGLYYTSGGSLCAFGEPLTEAAFRDENTVKDYHRRVGFIFQEPDVQ
                     LFCSTVREELAFGLLQLNFPSETIEKRIGEVSGMLRIEGLLDKTPFRLSGGEKKKVAI
                     ASVLILNPEVMILDEPTNGLDPRTRQWLTLLLEKLIGAGKTVVFSTHDLELAAAVSGR
                     SILFSEAHEVVYDGATRPLLSNAELLKSVNLVSENYRGVE"
     gene            11702..12997
                     /locus_tag="VHR42_05495"
     CDS             11702..12997
                     /locus_tag="VHR42_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3026663.1"
                     /translation="MGSIASDSYNQNIQGVAAKMIHPAKNLRQAVCKHFPKLHQSFHS
                     DRDFNLFLAVGFFSGISSGITTSCFNNYLNDVYHLSEAARGIVEFPRELPGALLIFIF
                     AVLNFVSDIKLAAISMILASLGMLGLGVLSPGFAFMLIWMVVLNLGTHLFMPLAPGIG
                     MNLSKQEQYGVRLGRYNAYNLTAAIIGYLIVWLGFQYFHISYTFTFAVASVCYLIAGG
                     ILLLMKPQKTRRPKTRFVIKKKFTLYYLLSIVNGARKQIFLTFAPWVLIKVYHLGTPS
                     FALLGFVIAFVSITTRTIVGKAIDKLGERIILSAEAIVLILLCMGYAFSADLFPVSVA
                     VIITAACYVLDNSLSAVEMARSTYVRKISEDPSEVIPTLSTGTSFDHVVAMTIPFFGG
                     LIWTAFSYKFVFILAALIALSNLFLSMRIKTPPARPIQE"
     gene            complement(13113..14153)
                     /locus_tag="VHR42_05500"
     CDS             complement(13113..14153)
                     /locus_tag="VHR42_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HEX3026664.1"
                     /translation="MIILLILLVLTAVLPLAVKPIEKNIEIFLGMIGILAAAVSGTLT
                     TKNISLIFHNRYLYMITGAVLTVSILFTLAGQRIRRLINKTLSAIPLRAAVFLLVVGL
                     GLLSSVITAIIASLLLVEIASALPVDRDKRVKICIISCFSIGLGAVLTPLGEPLSTIV
                     TSKIQGDFGSVFRLLGGAVIPGILVLGIIAAVYVGGSRQHDPGLTEDGLGAMMEKEDA
                     RAIFKRTAKVFLFMISLELLGFGFEPVIDGYIVHWGNTALYSANTISALLDNATLAAA
                     EIGFGMTNMQMKYTLVSLTVSGGMMVTGNIPNIVTASKLKISMKEWFAFGIPLGAVLL
                     TGYYLLLSHLPV"
     gene            complement(14221..15807)
                     /locus_tag="VHR42_05505"
     CDS             complement(14221..15807)
                     /locus_tag="VHR42_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX3026665.1"
                     /translation="MKKYRRFLGATALTILLLSLTTVALAAGADPTGASYTAGAGGKT
                     LGEVAETAGRAQLSANFIWVMITGFMVFFFQCGFAMVETGFCRGKNAAHTMTMNFMVF
                     LVGAVGYFLCGFALQFGGSGGAAGLGTGGSVLNGMLSIPGLGGILGYKGFFLQSGNTY
                     DAGIYALFFFQMVFMDTTVTIPTGSMAERVKYSAVVISSFFIAMIYYPLFGNWMWGGG
                     WLSTLGKNFGLGHGGVDFAGSSVVHAMGGAAALAGAMVIGPRIGKFKKDGKAVTFPGH
                     HVPMAIIGTIILFFCWFSFNAGSTMNAGDFRLAVVATNTMIAGAIGGLAAMFYMWIRH
                     GKPDPSMTANGGLAGLVAITAPCAFVNAISAFVIGLIAGILVCLSVSFVENKLKLDDP
                     VGAVSVHLINGVWGVIATGLFADGKYGDGLNGVSGGVSGLFYGNAGQFAAQMIVVGVI
                     IVYGFGTMLLWYKVLDRVWGLRVSPEIELEGLDRDEMGVYGYPDFKMLRSAELDSTMP
                     DDAPVRELKNIKYRFRIKVR"
     gene            complement(15845..16147)
                     /locus_tag="VHR42_05510"
     CDS             complement(15845..16147)
                     /locus_tag="VHR42_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026666.1"
                     /translation="MKSQINKDGSKFGKRAAVLFLFTMSLAITLSGIIFSFYALLNQI
                     SFRVANMEVSGVLFGLLTFYLGIRYLMSVIHLQKEIERTHSKFSWKNFKKRHSAGV"
BASE COUNT         4184 a         4355 c         3799 g         3986 t
ORIGIN      
        1 ttcaatggga attgtcattt tgcccttcct cactatttga atcctgcaat tggacgcagc
       61 attgctccgg cgggatatgt cctgccggag caataccttt attttaatct cggaataaga
      121 aagccttaca gcgcaggcac gatgcccatc taatcctctt ttgtgatcgg tttgaaacct
      181 tcgccgatca cttcgctcgc ttcgcagctt acgataaatg cgtttggatc ggcattgcgt
      241 acgatgcttc tcagctttgc cgtctcgggc cttcttaccg tgcacattaa cacgtccttt
      301 tcgtgcccgg tataaaatcc ccgtcctttc agcatggtga ccccccggct cagctcatcc
      361 ataatcttat tcgcgatcat ttcatttttc tccgaaatca ccagcatcat ctgaccggtt
      421 tccgatccgt aaatcatcgc gtcaattact ttcgagcagg taaatatcgt tataaacgcg
      481 tagaggccgc tgtcgacact tttaaacaca aatgtagata atatgacgat taaagagtcg
      541 ataaccatca tcatcttgcc catgggaacg tgcttaaatt tgagtctcat cagccggctg
      601 ataacatcgg agccgccgga tgtcgcgccg cgcagaaata caagtccgag gccgacaccg
      661 gacaggattc ctccataaag ggcggccagc agcatactgt gggtatatgt tggaacaaaa
      721 agcgccattc cgtccacaaa taccgaagtc aagaccgtac acaacagcgt ttttacaata
      781 aactccctgc tgatgagttt aaaactcagc accaatagag gaatattcaa tatcagatac
      841 atcagaccga tcggggtatg tatcaggtaa ttcaaaatgg tcgcgatacc ggtgattccg
      901 ccgggagcaa tgttgttggg agcggtaaaa acagccacgg aagccgaata aataatactt
      961 ccggccaaga taaatataaa gtcccaaaaa tattctttgg ctttttccgg ggaaatcttt
     1021 tgggatattt tattgatact gttatgagtc atgggaaccc tcctattaat cttttccgtc
     1081 cctatgatac cctgcggcca aaaacctcgc ggcgcgggcc cgaccgggga aacaggaacc
     1141 taaagcgata taaattcaat ccgccggctt acaataattt ttccggtctg tcaggcggtg
     1201 tcagctgttt ttgcttttca ccaccgcgtc gaagatctca tcgatcctct catgcttttc
     1261 atgcagaaac agataaccga aaagggcctc cagccctgtc gcgtaacgat attcaatcgg
     1321 gtcggcattt ttaggaatgt gggcggagtt ggagttgcgc ccccatcgga gaatttcctg
     1381 ctcctcttcg gtcagcatgg accaaattcc ccgcacggac tcagcctgag ccgcagccct
     1441 gacataatcc acacatttct tatggagctt tttcgccaca atgttgccgc tgcgtatcag
     1501 atgcgctctg actttaagct caaaaaccgc gtcacccaga aacgcaagcg taatcgggct
     1561 gaaaagccgg taggggtcag tcgccggctt ttgttgatcc atgaccgaat tatctgacat
     1621 actcaaactc cacatcataa atactgaccg tatccccctg ctgaattccg gccttttcca
     1681 atttttcgat cacgccgctg tttttaagaa cccggtgaaa atactgcagg gattcataat
     1741 cgtcaagatt caccgagcca atcacctgta ttagccattc actctccaca aaatagacgc
     1801 cttcgtggtt tgtgatctga atttcgcgct ccttttgagc ggaaaaatca tcctgcggca
     1861 gcggctgagc ctcataccgg ataatcggag gcagttccga gagccttcgc agggtaaaat
     1921 ccatcagttc cttgacgccg ctgcgggtcg ccgctgaaat cgggaaaaac acaaggccct
     1981 tactttccac ataatcccta aaatgcgaaa tctgctcctg ttcggcaatg tcgcatttat
     2041 ttcccgccac aatctgcggc cgtttggaaa gttcctcgct gtatttggca agctctccgt
     2101 ttatgtcttc aaaatcgctt acagggtcgc ggccctcgct tccggagaca tccacaatat
     2161 gaatcagcag ccggcagcgg tcgacatgcc ttaaaaattc atggccgagc ccgacgccct
     2221 cgcttgcgcc ttcgatcaga ccgggaatat ccgccatgac gaaagatttt tcctggtgat
     2281 aaaccacgcc gagaaccgga gtaatcgtgg taaaatggta gttggcaata ttgggtttgg
     2341 cttcgctgac gatcgacagc agggtcgatt tgccgacatt cggaaaaccg agcagcccaa
     2401 cgtcggccaa tagcttcagt tcaagcacaa tctctttttc ttcccccggg attccgctct
     2461 tggcgaaacg cggaatctgc ctggtcgggg ttgcaaaatg gcagttgccc catccgcctt
     2521 taccgccttt cgcggcaaca taaggttcgt cggacgacag atcggcaaca atcaggccgg
     2581 tcttcgcgtc tttaatcagc gttcccctgg gcacttttat ctcaagattc gcaccagcct
     2641 tgccgaaaca ttttttttgc tttccgtttt caccgttcgg agcctcatat ttctttttaa
     2701 acctgaaatc ggcaagcgtt gaataattgt cgtcgacgat aaaaataaca tcgccgccct
     2761 taccgccgtc gccgccgtca ggacctccgg ccgcaacata tttctcccgt ctgaaagaaa
     2821 cagcgccatt tccaccgttt ccggcccgga ttgtaatttt agccttgtct acaaacatat
     2881 cggtttaccc ttccccggct atggattccc acgcgttccc gcatgacaga ctgattctct
     2941 ccatttctgc cgtttccatg aagtctcata tatataataa tggtttaaag tttaaagtca
     3001 aattttattt ctcaaaataa acaaattgtt aataatcagc ccgtttatag ttattttccc
     3061 atttctgtga tataataatt ccgatttatc aatgaaataa aaagaatttt tacgagcctt
     3121 ttttacgact ccaaatcggc cgctcctcag gaaaatcagc gaggattttc cggcggtcgg
     3181 ggccgatttg aaatgacggg gggttttctt acggaactgg agcgcaaaac actgagtgat
     3241 aatatcaact ttacggcaat acgcgacgga agattcaaaa acaaccagat ttccgtcaac
     3301 tttctgatgc cgctgagaaa agaaacggcg gcgcttcatg cgctgatacc gcttgtgctc
     3361 aagcggggct gcagaccgta cccaaacatg acgcttctca acaggcggct taaggagctt
     3421 tatggcgcga gacttgacgg aggagtcggc aagcggggcg aaatgcaggt gatttcccta
     3481 tactgcgccg cgctcgacga cacctatgcg ctggagggag aaacactgct tcgcgaatgc
     3541 gcgggcctgc tgaaatccgt cctgttcgac cccatcgtga cggatggagc gctttctgcc
     3601 gaagatgttg aggttgaaaa gaaaaacctt gctgatttaa ttgaatccca gctcaatgat
     3661 aaacgtcatt acgcctcgct caaagcaaaa gaagccatgt gcgccggcga agcttatggc
     3721 gtgagcgaat acggaaccgc ggctcaggcg cttgccatca cgcccgccgc gctgtatgag
     3781 gcatatcaga ccatgctcaa aaccgcggga gtcgaaatta tccttgtcgg gccggctgat
     3841 aaaacagaat gtgagtctct gtttgccgaa gcgttcgctg cggtcagccg cggcaaactt
     3901 ctccgctgcg tcaccgacgt catccgggaa cccgggccgg ttcggaccgt tgaagagcgg
     3961 ctcgcggtca atcaggcaaa gcttgtcatg ggattccggg ccggaaccgc cgcaccggaa
     4021 agcgaagcgg acacgatgca gctcgcaagc gcaattctgg gcggaacccc acattccaaa
     4081 ctgtttgaaa atgtccggga aaagcttggc ctgtgctatt actgcttttc ctattttgaa
     4141 cgtatcaagg gccttgtttt cgtggaggcc ggtattgagg aacaaaatta ccaaaaagca
     4201 aaagatgaaa ttctcaacca gctcgaactg cttaaaaagg gagaattttc ggatgacgag
     4261 ttgagctcgg ccaagctgag tctggaaaac tcctaccggg aactcaacga cacgctttac
     4321 gatatttcca gctggtatct cagccaggcc gttaccggcc agattcggac gcctgatcag
     4381 accgcccggg ccctcgctgg aatcgaccgc ggacagatcg cgccaacggc ggcgaaaatc
     4441 aaactcgata ccgtttatct gctggccggg gaaaggaagg attaacgcat ggatttggaa
     4501 ctgatcgaga gcaaaaggct ccatgaaaag atctattata cccgccatcc ctcgggactg
     4561 gatctgtttg tatctccgaa aaagggctat tcttcccaat acgccgtgtt tggaacaaaa
     4621 tacggctcga ttgacaactg ttttgaattc gggggtgaaa ccgtccgcgt tccggaggga
     4681 atcgcccatt ttctcgaaca taaactgttt gagagtgagg atggcaacgc gttcagccga
     4741 tacgccaaaa cgggcgcgaa cgccaacgct tacacttcct tcgaccggac atgctacctt
     4801 ttttcggcta cttccaattt ttacgaatcc tttgaaattc tgcttgattt tgtccaaaac
     4861 ccctacttta ccgaacaaac cgtgcaaaaa gagcagggta tcatcgggca ggaaattaaa
     4921 atgtatgagg acgaccccgg ctggagggta ttttttaacc ttctgtcggc cctctatcac
     4981 acacaccccg ttaaaatcga cattgcgggc acagccggct ccatcgccca aatcacctcg
     5041 gatcttcttt acaaatgcta ccgcgctttt tacaacctga ataacatggt gctctgcgtc
     5101 gccggagatg tcgatcccga acgcattctc aaaacggcgg acaggctgct caaaactccg
     5161 gccccgtcaa aagtcagaag tattttcgac cctgagccgg ataccattgt taagcagcgc
     5221 gtcgagcaaa aactatcggt gtcggttccc ctcttctgct tcggattcaa aggctctccc
     5281 gccgagggcg ccgaagcggc cagagcggag gccgagtcct ccattctgct tgaaattctt
     5341 ggtggcgacg cctccgggtt ttaccgcagg ctttatgaca agggactgat caattcctct
     5401 ttcggccttc agtattttag cggccgaagc ttcgcgtcaa ccgtggtttc cggagaatcc
     5461 agagagccga agcttgtgat ggaggaattt ttaaaagaag tcaagcgcct gaaatccgaa
     5521 ggaattccct ctgaggaatt tgaaatggcc aaaaaggcct attacggcag gcttgcttcc
     5581 cattttgaca gcgtcgacaa cgtggccaac gggatcatcg gctctcattt tacagggatg
     5641 ggcccgtttg aaattgtcga ggcggcggca agagttactc cggagaatgt ggcaaaaaga
     5701 cttgcgaata ctttttcaga agagaaagcg gctctttcag ttatcaatcc gctttaaaca
     5761 agataccgca aaacaggaga tgatcaaatg gaaagcccca ttacttttcc gggactgcat
     5821 ctgacgttca atattcagag gcaggcgctg tcttttggaa aattcggaat ctactggtac
     5881 ggcataatca ttgcgaccgg tcttgtaatc gccatcctct atgggctcaa gcgctcaaaa
     5941 tcgtttggta tcacacagga cgatctgatc gatgttatcg ttttttcgat tattgcggcc
     6001 attatcggcg cccggctcta ttttgtgctg ttttacatcg acccgagctc cggcaccaac
     6061 ccgtattttt cagacccggc ttcgattttt aacgtcagaa gcggcggttt ggccatctat
     6121 ggcggaatca tcggcgcttt tgtctgcgca acaatcgtat gtaaaattaa aaagatcagc
     6181 acgggcgcgg tatttgatat tgccgccctt ggttttctga tcggccagag tatcggcagg
     6241 tggggcaact ttatcaaccg tgaggcttac ggtgccgcca cctccctgcc gtggagaatg
     6301 gtagtcgaca gcaccggaac aggctatcat ccctgcttcc tttatgagtc gctctggtgt
     6361 attttaggat ttatcattct acatatctat tcaaaacgca ggaaattcaa cggtgagatt
     6421 ttcctgatgt atctgatgtg gtacagcttc ggccggttct ttattgaagg tctgagaacc
     6481 gacagcctga tgctcggcaa tcttagaata tcccagcttg tggcggggct tttgtttgtt
     6541 gccgccggca ttttcatcta cctcaaaaga cagaaaagca atggcctgac ggacgatcag
     6601 ggcgaataca agcgcgttta tgaggacgcg gccgaggccg tacggcaggt cagcgacaat
     6661 tcagacgaaa cccaaagcaa tgacctgccc gaagatatga aaaatgacag ccgggacgac
     6721 ggcggggagg aaaagtaatg gcagcggtta tcatcaatgg aaaagaagta tcggcaaagg
     6781 ttaaaaacca gattaccgag agcgtccgtg aactgcgcga acaacacggc gtcactcccg
     6841 ggcttgcggt cattattgtt ggaggaaacc ccgcttcaag ggtatatgtc aacaacaagg
     6901 agaaggattg cgccgaaacc ggcatccatt cggaagaata cgcgctggat gaatccactt
     6961 cacagcggga tctgctgcag ctggtggaca atctcaacaa gaacccggca ataaacggaa
     7021 ttctcgttca gcttccgctt ccttcccacc tcaatgagga ggaagtgatc aacgccatca
     7081 gccccgaaaa ggatgtcgac gcttttcacc ccgccaatgt cgggcggatt atgatcggcg
     7141 acttcgattt tctgccctgc acgccctccg gtattatgga tatgctggat gattccggta
     7201 ttgaaatcgc cggaaaggaa tgtgtggtgg tcgggcgaag caatatcgtc ggcaaaccga
     7261 tggcaatgct gctgcttcac cgcaacggga cggttaccgt ctgccattcc cggacgaaaa
     7321 atctggagga agtaacgcgc cgcgcggata ttctggtcgt cgcggtggga aaagcacatt
     7381 tcatcaccgc cgggatggta aagcccggag cggtagtcat tgatgtgggc atgaaccgtc
     7441 tggataacgg taaactgacc ggcgatgttg attttgacgc cgtcgcaccg attgcttcct
     7501 atatcacgcc cgttccgggc ggggttgggc cgatgacccg ggccacgctt ttgaaaaaca
     7561 cgctcagggc ggcaaaattg caaaacggaa tttgacagcc cccctgctca tgtgctataa
     7621 tgctgtatgc cgcatgctat gggcttttta aaagggcttt gccctgcccc ccgcagcgag
     7681 acttataaaa aggcaggtgc gttttagaaa tgtacgctgt aattgaaacc ggtggaaaac
     7741 agtatcgtgt tcaggaaggc gacaccatct ttattgaaaa gctcggctct gacgaaggaa
     7801 tggaagtttc ttttgataaa gtcgtcgcgc tcggcaaaga cgagggcctc gaagtaggcg
     7861 ctccgtatgt cgacggtgta aaggtctccg ccaaagttct gaagaacggc aaaggcaaaa
     7921 agatcaaagt ctttaagttc aaggcaaaaa aaggttaccg caaccgcaaa ggccacaggc
     7981 agccttatac aaaggttcag atcgaaaaga tcgtcgcgta atgattacgg ccaggttttt
     8041 tttaaaaaat ggagccgtcg cgggttttga agtcagcggc cacgcgggta tggatgccca
     8101 gggtcatgat gtagcctgcg caagcgtgac atcggcggtt cagatggcgg caaacaccgt
     8161 gactgaaatt atcggcgcaa aagccgggat acaagtcgac gaagcattga ttaaaatgga
     8221 attagccgat acaaatggcg cggaatccga aaaggccgtc caggcggttt taaagggaat
     8281 gcgcctgcat ctgaaactgc tgactcagga gtttcaggag tttatccgag tagaagatat
     8341 ggaggtgcag tcaaaatgat taagttgaat atgcagtttt ttgcacataa aaagggcgtc
     8401 ggttccacaa agaacggacg tgacagtgaa tccaaacgcc ttggcgtaaa gcgtgcggat
     8461 ggtcagtttg ttctggccgg aaacattctc gtccgtcagc gcggaaccaa gattcatccc
     8521 ggtgccaacg tcggcagagg ctctgacgac actcttttcg ctctgagcag cggaaaagta
     8581 aggtttgagc gcgtcggccg tgaccgtaaa aaggtcagcg tctacgttcc cgagcaataa
     8641 gaacaacttc aatcaaaatc cccgtcctaa aagggcgggg attttttttg tttcatttaa
     8701 gttaagctgc tttttaattt aagcttcttt taccactttg gcgaacaatt tttcaaagca
     8761 ataattaata atcacactgc gtttgatgat tccgataaat ttgttgttat catccacaac
     8821 agggataaaa ttctgattga ttgcagtcgt cagcaggtct tccatatcgc agttgatatg
     8881 aaccggctta ttgctcacat gcagctgaac atctgagatc agtattttgc tggtattctt
     8941 aaaattaagc ctcggcgtat tttttaattt ccacagcaaa tccccttcgg tgagggttcc
     9001 gacgtattga ccatcctcgc taagtatggg aattgcgcta tatcggtgat attccattcg
     9061 ttccatcgcc tgtctcatcg tggaattaaa cagctgaaaa acgacatctt ttttgggggt
     9121 gagaaaaaaa gcgatattca ttcatagatt cttctttctt actcagcttt gtattattca
     9181 cctaatctat tatagcatct tttgagcaaa ttaaataaac aaattgtaaa ctttaaacag
     9241 ttgctctctg gtaactatcg ggtaagccga tcgtccgggt cgacaatttc aattcccgca
     9301 ttagcggccg tatcctggaa gatggtccta tcatggcgat cggtgaggcg cacttccagc
     9361 gttccattcg agcattccct gatccgcccc gccattccgt cctcagcggg agctgccagc
     9421 tcgccgccct gacctgagta agctttgacg tcgatccgaa aaaaaggcga ctcggcccta
     9481 agaaaaatct ctccgttttt ttgaaagaat cttgtcactg aggcggccga ataggtggca
     9541 aaccggtaaa atttacctct gaaatacaaa aaagcaatca gcccgcggat actctttccg
     9601 aaaaccggca catccgcgta agagagcatg aaagatgcat cgctgtgcgc aaaccgggcg
     9661 gattccatcc aaacgtacga ccgggggaag aaccgtcccc aatctttttc aatataacct
     9721 gttccgccgt caaaatccat cgtacgcccg ctgatgttaa ggctgccggc caggctgctt
     9781 ttgacgcaaa ccacaccgtg tctgcattcc agataaggca gaagcgagaa tggccccatg
     9841 atgtcacgcc cccaaaatcc ggccgaatat tccgtgggat ttcggaactc caccgttccc
     9901 ctcaccgcga tttcacgccg gatatcaagc tccatgcggt cattggaaaa caggttgtct
     9961 cccacgcaaa aaagaagctc gccaccagag ggcacgaact tcctcgccgg ataatcaaag
    10021 taaaaggttc gttccaaaaa ggacgcgatc agctgcacga acgcgtgcgc gccgttcttt
    10081 gttttgcaaa tacccggaat cagagcgacg gtttcgccgc ctgctgtctg aactctgtaa
    10141 taccacccct caaaataagg ggctttttgt ccgctcccct gataggcttc cggtttgagc
    10201 ggcattccga taagattcat tgatttttcc cccataccat cgtttatatt tagtgtgtga
    10261 aaagcgatga taaaaaaccg ggaaattcaa tcaatttatt cctattttga acaacagagg
    10321 tttttaagca ataaaacagc gtcatcagcg taagcagaca agggtttgga aaccgcatcc
    10381 accacataaa gcccgcaccg gttaagaggg atcagaatct tctttgccgg gctttcggct
    10441 atcgccttgg ccataagcgg ggtcagctcc ccccgcatag aattggatgc gataattccg
    10501 acgcttccca tgattacatc cgctttggaa gcgttgaaca ccaccgcatt ttccccgctc
    10561 gcgccttcat ccgctcccgc ctttaacatc agactggtcg cgagcgcatt ggtgccgagc
    10621 gcaagtatct caatttggcc gccaaactcc gcccttgtct tttctacaat cagttttcca
    10681 attccgccgc cctgaccgtc aaccaccgcc aaaagcatag gatcaccctc ccaaaaaatc
    10741 gggtgcgtca ttcgacgccc ctgtaatttt cagacaccag attaacgctt tttaatagct
    10801 ctgcattgct caaaagcggc ctggttgctc cgtcgtaaac aacctcgtgg gcttcggaaa
    10861 acaggatgga gcgcccggac accgccgcgg ccagctcgag atcatgagtg gaaaacacga
    10921 ctgttttccc cgccccgatc aatttttcaa gcagcaatgt cagccattgc cttgttctgg
    10981 ggtcaagccc gttggtcggc tcgtcaagaa tcataacctc cggattcaaa attagaacgg
    11041 aggcaatcgc aaccttcttt ttttctccgc cgctgagccg gaatggagtt ttatccagca
    11101 agccctcgat tctcagcata ccagagacct caccgattct tttttcaatc gtttccgacg
    11161 gaaaattcag ctgcagcagc ccgaacgcca gctcttccct gaccgtactg caaaacagct
    11221 gcacgtccgg ttcctgaaag ataaagccta ctcttcgatg gtaatcctta actgtatttt
    11281 catcgcggaa ggctgcctcg gtcaaaggct ccccaaatgc gcagaggctc cctccggaag
    11341 tataataaag accggtaagc atttttaaca gcgtcgactt gccgcatccg tttgcaccga
    11401 gaatactgac gccctcaccg ggaccaataa caagattgat atcttttaat accatattct
    11461 gacccgggta ggcaaattcc acatgattca attcaaaaac tgccgcgttt gatttatttt
    11521 tcaacgttca caactcccct cccaggacaa aataaagccc atgcccgggc atcttttgcc
    11581 cctcatgggt aactttaccg cgtactctca aattttcggc ccacgtatca agcctatttt
    11641 atcatggatt atcaccccgg tcaattgtaa ttcggcgccg ctgccgctat aatagaagta
    11701 attgggctca atcgcgtccg actcttacaa tcaaaacatt cagggagttg ctgccaaaat
    11761 gatccatcct gcaaaaaatc tacgacaagc ggtctgtaaa cacttcccga aactgcatca
    11821 atcgtttcat agcgaccgtg attttaatct ctttctcgcg gtcggttttt tttcgggtat
    11881 ctcaagcggc atcacaacct catgcttcaa caattatctt aatgacgttt atcatctgtc
    11941 ggaggcggcc agaggaatcg tcgaatttcc gcgcgagctt cccggagccc tgctgatttt
    12001 tatttttgca gttctgaatt ttgtcagcga catcaagctt gcggcaatca gcatgattct
    12061 cgcctcactt gggatgctgg ggctcggcgt tctttcgccc ggatttgcct ttatgctgat
    12121 ctggatggtc gtgctcaatc tcgggacgca tctgtttatg cctcttgcac cgggcatcgg
    12181 aatgaatctc tccaagcagg aacagtacgg ggtccggctg gggcggtata acgcttataa
    12241 cctgacggcg gcaattatcg gctacctgat cgtctggctg ggttttcagt atttccacat
    12301 ttcctatacg ttcacttttg ccgtcgcttc ggtgtgctat ctgattgcgg gcggcattct
    12361 tctgctgatg aagccgcaaa aaacccgaag gcccaaaaca cggtttgtga ttaaaaagaa
    12421 atttacgctt tattatttac tcagcatcgt caacggcgcc agaaaacaga tttttctgac
    12481 ttttgcgcca tgggttttaa tcaaagttta tcatctcgga accccttcct tcgcgctttt
    12541 gggttttgtc atcgcatttg tcagcatcac aacaagaacc atcgttggaa aagcgattga
    12601 caaactcggt gaaagaatca ttctgtccgc cgaagcgatc gtgctgattc ttctttgtat
    12661 gggctacgct ttttccgccg acctcttccc ggtttccgtc gccgtgatca tcaccgcggc
    12721 ctgttatgtc cttgacaact cgctgagcgc ggtggaaatg gcccggtcga cttatgtgag
    12781 gaaaatctcc gaagacccct cggaggtcat tcccaccctc tccaccggca ccagttttga
    12841 ccatgtcgtt gccatgacaa tccccttctt cggggggctg atctggacgg cgttcagcta
    12901 taaattcgta tttattctgg cggcgctgat tgcgctctcg aacctttttc tttcgatgcg
    12961 aatcaaaacg ccgccggccc ggcccattca ggaataagca tctgttttcc ttacggagag
    13021 gctgcccttt cgggcagcct ctccgctttt tccgcgaaca aaagcatttg acaatatact
    13081 tttagcaaac ggtttcaaat gctcccctcg ctctaaaccg gaagatgcga caggagcaga
    13141 tagtaacccg tcagaaggac ggctccaagg ggaattccga aggcaaacca ttccttcata
    13201 ctgattttga gtttcgacgc ggtaacgata ttaggaatat tgcctgtcac catcattccg
    13261 ccgcttacag taaggctgac aagcgtatat ttcatctgca tattcgtcat cccaaacccg
    13321 atttccgccg cggcaagggt ggcattgtcc agcagagccg aaatagtgtt tgccgaataa
    13381 agcgcggtgt tgccccagtg aacgatatag ccgtcaatga ccggctcaaa accaaagccc
    13441 agtagttcaa gggaaatcat aaacaggaaa acctttgccg tccgcttaaa aattgcccgg
    13501 gcatcctcct tctccatcat cgctcccagg ccgtcctccg tcagccccgg atcgtgctgc
    13561 ctggaaccgc cgacgtaaac cgctgcgatg atccccaaaa ccagaatgcc agggattacc
    13621 gccccgccca aaagccgaaa gacagacccg aaatcccctt gtatctttga agtcacaatt
    13681 gtggaaagcg gctcgccgag aggagtgagc accgcgccta atccgataga aaaacaggat
    13741 atgatacaga tttttaccct tttatccctg tccaccggaa gagcggatgc gatctcgacc
    13801 agcagaaggg acgcaataat cgccgtaatc acgctggaga gcaggcccaa gcccacaaca
    13861 agcagaaata ccgcggccct cagcggaata gcggacagcg ttttgttgat caaccgcctg
    13921 atcctttgcc ccgcaagagt gaacaggatg ctgaccgtga gaactgcgcc ggtaatcata
    13981 tagagatacc ggttatgaaa gatcaggctg atatttttcg tggtcagcgt tccggatacc
    14041 gcggcagcga ggattccgat catccccaga aatatctcaa tattcttctc gattggtttc
    14101 actgccagcg gcaggactgc ggtaagcacc agcagaatca gcagaatgat catcaaagca
    14161 gaaccctccc ctgttgtcta tacagatcga acgtaccggc ttcctgaaaa aatccgtggt
    14221 tcagcgcact ttgatccgga accgatattt gatattcttc aattctctga ctggagcgtc
    14281 atccggcatc gtactgtcaa gctcggcgga acgaagcatc ttgaaatccg ggtatccgta
    14341 aacacccatt tcatcccggt caagaccctc cagttcaatc tccgggctta ctcttagccc
    14401 ccagacccga tccagaacct tataccagag aagcatcgta ccaaaaccat agacgatgat
    14461 gactccgacg acaatcatct gcgccgcaaa ctgaccggca ttgccgtaaa acaatccgga
    14521 tactccgccg gaaaccccat tcagcccatc accgtatttt ccgtctgcaa acaatccggt
    14581 ggcaatgacg ccccagacgc cgttgatcag atgaaccgaa acagcgccta ccgggtcatc
    14641 gagtttcagc ttattctcca cgaaggagac actcagacag accagaattc cggcaatcag
    14701 accgatcaca aaagccgaga tcgcattaac gaacgcgcag ggagccgtaa ttgccacaag
    14761 cccggccaga cctccgttgg ccgtcatgga cggatcaggt ttgccgtggc ggatccacat
    14821 gtaaaacatg gcggcgagcc cgccgattgc gccggcgatc atagtattgg ttgcgacaac
    14881 cgccagcctg aaatcacccg cgttcattgt ggagccggcg ttgaaggaaa accagcagaa
    14941 aaagaggatg attgtgccga tgatcgccat cgggacatga tgccccggga atgtgaccgc
    15001 tttgccgtcc tttttgaatt tcccgattct cgggccgatt accatcgcac cggccagcgc
    15061 cgccgctccg cccatcgcgt gaaccaccga tgaccctgcg aaatccaccc cgccgtgccc
    15121 aaggccaaaa tttttgccca gagtcgaaag ccatccgccg ccccacatcc agtttccgaa
    15181 aagcgggtaa taaatcatcg cgatgaaaaa tgatgaaatg accacagcgg agtatttcac
    15241 cctctcggcc atcgagccgg tcggaatcgt aaccgtagtg tccataaaca ccatctggaa
    15301 gaaaaacagg gcatagatac cggcatcata ggtatttccg ctctgaagga aaaacccttt
    15361 gtaaccgagg atccctccaa ggcccggaat ggagagcatt ccgttgagca ctgagccgcc
    15421 cgtgccgagg cccgcggctc cgcccgagcc cccgaactgg agggcaaaac cgcaaaggaa
    15481 atagcccacc gctccaacaa gaaaaaccat aaaattcatc gtcatcgtgt gggccgcgtt
    15541 tttgccccgg caaaagccgg tctccaccat tgcaaagcca cattgaaaaa agaataccat
    15601 aaagcccgta atcatgaccc agataaagtt ggcgctcagc tgcgccctgc cggccgtttc
    15661 ggctacctcc ccgagcgttt tgccccccgc tcccgccgta taagacgctc cggtcgggtc
    15721 agccccggcc gcaagcgcaa ccgtcgtcag agatagaagt aaaattgtta atgccgttgc
    15781 tcccaaaaac cttctatatt ttttcatcat aattccccca aaatatcgtt acgccaaccg
    15841 ttgatcagac gcctgcggaa tgccgctttt taaaattctt ccatgaaaat ttactatgag
    15901 tccgttcaat ctctttttgt aaatggataa cggacatcag ataacgaatt cccaaataga
    15961 atgtcagaag accaaacaga actcctgata cttccatgtt ggctacacgg aaggaaattt
    16021 gatttaacaa cgcataaaaa ctgaagataa tacccgacag ggtaatcgcc aacgacattg
    16081 taaaaaggaa taggacagcg gcccttttgc caaacttact gccgtcctta tttatttgac
    16141 ttttcatcaa accacccgct tttcaatact ttcgcaaaaa tttcacatgt gtcgcgacaa
    16201 gcccataata aaatttctgc ccaattttaa aataaaaaga cgcctgtatt aatccggccg
    16261 aattaataca ggcgtctttg cccaacaact atttattttc ataatttcta tttttattat
    16321 aaca
//
LOCUS       DATALY010000188         8753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_646000,
            whole genome shotgun sequence.
ACCESSION   DATALY010000188 DATALY010000000
VERSION     DATALY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646000"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..461)
                     /locus_tag="VHR42_05515"
     CDS             complement(45..461)
                     /locus_tag="VHR42_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3026667.1"
                     /translation="MLTGISTACLYPMETERALEVMLGLGFDCFEVFANCESELTDKY
                     IETLGKMMAARGARVYSLHLFTGSYEPFMFFSNYKRRFDDSIKQFRRYFRSARLLGAS
                     VVVFHGGHRNALMEIEEYCERFLLPAAGSPSPARGR"
     gene            624..896
                     /locus_tag="VHR42_05520"
     CDS             624..896
                     /locus_tag="VHR42_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026668.1"
                     /translation="MQHYLTDMVKHMKNMDRFSVAVIYHSLQLVCGLYAMALLFLALE
                     GTFGNYITVISCYKGALDTAPAVLIAALAAAFICDIAVKDRQSDRT"
     gene            complement(1007..2008)
                     /gene="secF"
                     /locus_tag="VHR42_05525"
     CDS             complement(1007..2008)
                     /gene="secF"
                     /locus_tag="VHR42_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX3026669.1"
                     /translation="MKKNTEIINDNSTRLNIDFISKRKVYYTISICLILAGILVSVIF
                     GVKLDIQFKGGIIIKYSYTGTIDKAKAQEIGKGVLKSDVSVQLNKDVVSNTQRLVLES
                     DTTSISEATQQKLYEAYNTAFSGNHIQKYESNDVSPEMGAQFFRQSILAVLLASTLII
                     IYIWIRFRHIGGLPAGITAFIALVHDVVMAFVAFSVFRIPLNDNFIAIALTILGYSIN
                     DTIVVYDRIRENRKLYGSKVHFKDIVNRSINQSFARSINTTLVTFFAIATICVFSVIF
                     NIESIRNFALPMMVGVVTGCYSTICIAGPLWVTWVSYKEKKEENSSKGNKKLKPKNA"
     gene            complement(2005..3282)
                     /gene="secD"
                     /locus_tag="VHR42_05530"
     CDS             complement(2005..3282)
                     /gene="secD"
                     /locus_tag="VHR42_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX3026670.1"
                     /translation="MYKRVGKPVFFVVLLVILAISYLSAFGVRSNYGDVSTAIIKGAQ
                     DMRFGIDIRGGVDVTFRPPKNFNATSDQMKAAEAIINQRLDNLKISDREVYTDTAHDR
                     IIVRFPWKSGEKSFDPESAIKELGATALLTFKAPDGTVVLSGSDVKNASPAYVQTTGQ
                     YVVNLTLSDTGKTKFAAATKKYLGQIITINMDNQTIESPSVKSEIDNGQAEIEGNFTS
                     ASATALANKINAGALPFKLETDNFSTISPTLGSNALNVMLLAGGVAFGLICLFMMLYY
                     RLPGVVACIALCGHLAGTILAISIPQFTLTLPGIAGIILSIGMGVDCNVITAERIKEE
                     LRTGKTLDGAIDAGFDRSFSAIFDGNVTVIIVGIILMWLGSGSVKSFGYTLICGVVFN
                     FVMGLMASRLMLKSVSRFSFARKDALYNGRAVK"
     gene            complement(3469..4899)
                     /gene="purB"
                     /locus_tag="VHR42_05535"
     CDS             complement(3469..4899)
                     /gene="purB"
                     /locus_tag="VHR42_05535"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX3026671.1"
                     /translation="MKNAYESPLGSRYASPEMQSLFSPDMKFKTWRRLWIALAKAEKE
                     LGLKIGDNQIAEMEAHKDDINYETAQAREKEVRHDVMAHVYAFGEQCPAAKPIIHLGA
                     TSCYVGDNTDVIIMREALLVLRRKILNVIAYLSEFAMKYRDMPALAYTHLQPAQLTTV
                     GKRSTLWINELLMDLEELEYRVSTLRLLGSKGTTGTQASFLELFDGDHDKVKKLEELI
                     AKEMGFEAVVPVSGQTYSRKMDSQVLSTLSGIAQSASKFSYDLRLLANFKEMEEPFEK
                     HQIGSSAMPYKRNPMRCERITALARYVIVDSLNPAFTSATQWFERTLDDSANKRIAVA
                     EAFLAVDAILNIYMNVADGLVVYPKVIHQRLMRELPFMASENIMMSAVKKGGDRQELH
                     EKIRQHSIAASRKVKEEGLENDLIERICADPAFGLDQNEIEGILNPVNFTGRSKQQVE
                     EFVESSVRPVLEKSRNILGEKADIKV"
     gene            complement(5016..6305)
                     /locus_tag="VHR42_05540"
     CDS             complement(5016..6305)
                     /locus_tag="VHR42_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019390799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3026672.1"
                     /translation="MSEYASLTKRELESEKADLEKKYRGFQQKNLALDMSRGKPGADQ
                     LNLSMGILDCINSSQPCRAQDGTDCRNYGLLDGIPEAKALFAQLLEVNPTEIIVGGNS
                     SLTMMYDTVARAYTHGVLHSEEPWCRQDKIRFLCPSPGYDRHFAICEHFGIEMIPIEM
                     RSDGPDMDAVKRLVESDETVKGIWCVPKYSNPEGVTYSDAVVDRFAALKPKAPDFRIF
                     WDNAYCVHDLGETSDPLKNILEACRKNGREDMVFLFASTSKISFPGSGVAMMAASCDN
                     VAFIKKQMAAQAIGPDKINQLRHVRYFGDIEGIRNHMKKHAAILRPKFKAVTEGLEKE
                     LGGTGLAEWTQPNGGYFISLNTPDGCARETVRLCGEAGVKLTPAGATFPYGKDPRDRN
                     IRIAPTYPPVGELKTAIELLCLCVKLAGVERLLSEKK"
     gene            complement(6555..7736)
                     /gene="larC"
                     /locus_tag="VHR42_05545"
     CDS             complement(6555..7736)
                     /gene="larC"
                     /locus_tag="VHR42_05545"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX3026673.1"
                     /translation="MKTIYFQCGSGISGDMFVAAMLDLGVDARVLRQSLMGLGLGEFE
                     IKITKSQKCGIACTDFDVVIKEEAGHVHRHLSDVEKIIDDSALSNFVKTLSKKIVAVV
                     ADAEGKVHGIDRSEVHFHEVGAADSIADIVGAAVCIEQIKPDRILFSPFVEGSGFVRC
                     AHGILPVPAPATAEIMARAGIPFEVSEQMGEMVTPTGAAIAAAVSDGFGRMPRIALQK
                     IGTGCGKKDFEKANILRAFLGETDERNGGADEIEVLETCIDDSTGEELGACVAALLEA
                     GAADAYYTPIYMKKNRPAYLLTVLCESRLVDEIVGIVFRQTGSIGLRIRTAQRITMAR
                     DFREVPSAYGPIRVKISRYGDIVKMKPEFESVQRAAREYGVTAGEVTRSAIEQAGRPG
                     C"
     gene            complement(7733..8500)
                     /gene="larB"
                     /locus_tag="VHR42_05550"
     CDS             complement(7733..8500)
                     /gene="larB"
                     /locus_tag="VHR42_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX3026674.1"
                     /translation="MDIKSLLEKVADGEIKPEEAYEKLKILPYENLGFARVDHHRQIR
                     SGFPEVIFCQGKTAEQTGRIAQDLAARGSNVLATRADRTAYEAIRSVLPDAVYHEQAR
                     AVTVTLISPRKIGKVAVCTAGTADIPVAEEAAVTAEAFGCRAERIFDVGVAGIHRLFD
                     RLEEIRSANVVIAVAGMEGALASVIGGLVDRPVVAVPTSVGYGSNFGGLSALLTMLNS
                     CSAGIGVVNIDNGFGAGCLAAQINRLAADEEHERVDG"
     gene            complement(8532..>8753)
                     /locus_tag="VHR42_05555"
     CDS             complement(8532..>8753)
                     /locus_tag="VHR42_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX3026675.1"
                     /translation="SQREERLDVLLSRMKELDLKPEDYGWYLDLRRFGGVEHCGFGLG
                     FERLIMYITGITNIRDVLPFPRTPGSAEF"
BASE COUNT         2078 a         2474 c         2047 g         2154 t
ORIGIN      
        1 cgagatgatt gaggccatgg gcgaagatat tgtcaacgtt catctcagcg accacgcgcc
       61 ggagagggac tgcctgctgc cgggagcaaa aacctctcgc aatattcttc aatttccatc
      121 aaagcattcc gatgccctcc gtggaataca accaccgagg cgccaagcag ccttgcgctt
      181 ctgaaataac gtcggaactg tttgattgaa tcgtcaaacc ttcgtttgta attggaaaaa
      241 aacataaaag gctcataact tccggtaaaa agatgaagtg aatataccct ggcgccccgg
      301 gccgccatca tcttgccaag agtttcgata tacttgtccg tcagttcgct ttcacagttt
      361 gcaaacacct caaagcagtc aaaaccaagc ccgagcatta cctcgagcgc cctttcggtt
      421 tccataggat aaagacaggc ggttgagatt ccggtcagca ttatatcact ctctgacaat
      481 atttttatat tgaaacgctc cagccgcaat gttaacataa taagtatcgt gctacggaaa
      541 tttcagattg gttggacact gctgcggtca agagcacacc tggcgacaac accgaccgcg
      601 ctgcagggaa agggcggaaa caaatgcaac attatttaac ggatatggtc aaacatatga
      661 aaaatatgga tcgcttttcg gtcgcggtca tttatcacag tcttcagctg gtttgcggcc
      721 tctatgcaat ggccctgctg tttctggcac tggaggggac gttcgggaac tacattacgg
      781 tgatttcctg ctacaaaggg gccctggaca ccgctccggc cgttctgatt gcagcgcttg
      841 ccgccgcctt tatctgcgat attgcggtca aagatcgtca gtccgaccgc acatagcgtt
      901 tgccccggcg ccttgtgtgg caccccgatt tgtccgccga cacaggggac cggcccacat
      961 ccgccagcct gccgaacagg ccgcccctta cgggacggcc tgttttttat gcattctttg
     1021 gcttcagctt tttgtttcct ttggagctat tttcctcttt tttctcttta taactcaccc
     1081 aggtcaccca aagcggaccg gcgatgcaga tcgtcgagta acagcccgtt accacaccaa
     1141 ccatcatagg cagagcaaaa ttccggattg attcaatatt aaagataacg gaaaacacac
     1201 aaattgtcgc aatcgcaaaa aatgtgacca gcgtggtgtt gatcgatctc gcaaaagact
     1261 ggttaatact gcggttgaca atatccttaa agtgaacctt gcttccataa agcttccggt
     1321 tttccctgat ccggtcgtaa acaacaatcg tgtcgttgat ggagtaaccc agaatggtca
     1381 gcgcgatggc gataaaattg tcgttgagcg gtattcggaa aaccgagaac gcgacaaacg
     1441 ccatcacaac gtcatgcacc agagcaataa acgccgttat tccagccggc agccctccga
     1501 tatgacggaa gcgaatccag atatagataa taatcagggt cgatgcaagc agcacggcca
     1561 gaatactttg tctgaagaac tgggcgccca tttccggaga aacatcattt gactcatatt
     1621 tctgaatatg attgccgcta aaagccgtat tgtatgcctc ataaagtttt tgctgtgttg
     1681 cttcgctgat gcttgttgta tcagactcca acaccagtct ctgcgtattg gagacgacat
     1741 ctttattcag ctgcacggaa acgtcggatt tcaggacgcc ttttccgatt tcctgcgctt
     1801 tggccttatc gattgtgccg gtataggaat acttgatgat gatgccgcct ttaaactgaa
     1861 tgtccagctt aacgccaaaa atcacggaga caagaattcc ggccagaatc aggcagattg
     1921 aaatcgtgta gtaaactttg cgcttgctga taaagtcgat gttcagccgg gtgctgttgt
     1981 cattgatgat ctcagtgttt tttttcattt cacggccctc ccgttataaa gagcatcttt
     2041 gcgcgcaaaa gagaaccttg aaaccgattt gagcatcagc ctcgacgcca tcaggcccat
     2101 cacaaagttg aatacaacgc cgcaaatcag cgtgtagcca aaagatttca ccgagccgga
     2161 gcccagccac atcagaatga ttccaacgat aataaccgtc acattgccgt caaagatggc
     2221 gctgaagctt cggtcaaaac cggcgtcaat tgcaccgtca agcgttttgc ccgttctcag
     2281 ttcctctttg atacgctcgg ccgttatgac gttgcagtca acgcccatgc cgatcgacag
     2341 gatgataccg gcgattccgg gcaaagtcag ggtaaactgc ggtatggaga tggcaagaat
     2401 tgtgcccgcg agatgaccgc agagcgcaat gcaggcgacc acacccggaa gcctgtaata
     2461 aagcatcata aacaggcaga tcaggccaaa cgcgactccg cccgccagca gcatgacatt
     2521 cagcgcattg ctgccaagcg tcgggctgat cgtggagaag ttgtccgttt ccagcttaaa
     2581 cggcagcgcg ccggcattga tcttgttggc gagcgccgta gcagacgcag aagtaaagtt
     2641 tccttcgatc tcagcctgcc cgttgtcaat ttcagattta acactcgggc tttcaatcgt
     2701 ttggttatcc atgttaatcg taatgatctg accgagatat tttttggttg ccgcggcgaa
     2761 tttcgtttta ccggtatccg acaaggtcag attcaccaca tattggccgg tggtctgcac
     2821 ataggcgggg cttgcgtttt taacgtcgct tccggaaagg acgacggttc cgtccggcgc
     2881 cttaaatgtc agcaacgccg ttgccccgag ttctttaatg gcggattcgg gatcaaatga
     2941 tttctccccc gatttccacg gaaaacgaac aataatccga tcgtgcgccg tgtcggtata
     3001 gacttcgcgg tcggaaatct tcagattatc aagacgctgg ttgatgattg cctcggcggc
     3061 cttcatctga tcactggtgg cgttaaagtt tttcggcggc cggaacgtca catccacccc
     3121 gcccctaatg tcgattccga aacgcatatc ctgcgctcct ttgataattg cggtgctgac
     3181 atcgccgtag ttggaacgca ccccgaaagc agataagtag gatatcgcca gaatcacgag
     3241 caatacaacg aaaaatacag gttttcccac acgtttgtac acaggctttc accttccaaa
     3301 gcattttgat ggcttaaagc caccgttcat ctccgcgtca ggcgcggaag ccgactccat
     3361 atcttgggaa cagatcggca aaacacgaaa aaaagttgcg aaacaaactc tattatatgg
     3421 tgaaattgtt gaaaagtcaa ccaaaccctg tcgaatcact gaaatatcct aaacctttat
     3481 atcggccttt tctcccagaa tatttctgct tttttcgagc acgggccgaa cagacgattc
     3541 aacgaattcc tccacctgct gttttgaccg tccggtaaaa ttgaccggat taaggatccc
     3601 ctcaatctca ttttgatcaa gcccgaaagc aggatcggcg cagattcgct cgatcagatc
     3661 gttttcaagg ccctcttcct tgaccttccg cgaggcggcg atggaatgct gacggatctt
     3721 ctcgtgcagt tcctgacggt ctccgccctt tttgaccgcg ctcatcatga tattttcact
     3781 cgccataaac ggaagctcac gcataagcct ctgatgaatt acttttggat acaccaccag
     3841 cccatcggcc acgttcatat agatattcaa aatggcgtcc accgcgagga atgcctcggc
     3901 cacggcgatt cgtttgttgg cggaatcgtc aagtgtgcgc tcaaaccatt gggttgccga
     3961 cgtaaatgcg ggattcagag aatcaacgat gacataacgg gcaagcgccg tgatccgctc
     4021 gcagcgcatg gggttgcgtt tatacggcat ggccgacgag ccgatctgat gtttttcaaa
     4081 cggctcctcc atctctttga aattggccag cagccgcaga tcatatgaaa atttgctggc
     4141 actctgggca attccggaaa gcgttgaaag aacctgactg tccatcttgc gggaataggt
     4201 ctggcccgaa acgggaacca ccgcctcaaa tcccatttcc ttcgcgatca gctcttcaag
     4261 ctttttcact ttatcatggt cgccgtcaaa caattccaga aaagacgcct gtgtccccgt
     4321 cgtacctttt gaaccgagaa gcctgagtgt cgaaacccgg tattcgagtt cctcaagatc
     4381 catcagcagc tcattgatcc acagcgtgga ccgtttgcca accgtcgtaa gctgggccgg
     4441 ctgcaaatgg gtataggcaa gggcgggcat atcccggtat ttcatggcaa attcggaaag
     4501 ataagcgata acgtttaaaa ttttacgtct tagaaccagc agcgcctcgc gcataatgat
     4561 aacgtcggtg ttgtcgccga catagcagga ggtggcgcca agatgaatga tgggcttcgc
     4621 ggccgggcac tgctccccaa acgcgtagac atgcgccata acgtcatggc gcacttcttt
     4681 ttccctggcc tgcgcggttt cgtaattgat gtcgtcttta tgggcctcca tttcggcgat
     4741 ctggttgtcg ccgattttca ggccaagttc cttttctgct tttgcaaggg cgatccaaag
     4801 tcttctccac gttttaaact tcatgtcggg agaaaaaagg ctctgcatct ccgggctggc
     4861 gtatctggaa cccagcggac tttcgtaagc gtttttcaat tctgcaaacc cccaaagcct
     4921 gtcaaccagg caatcttcgc tttttaacaa tttagtatag catatccggt tattaatttc
     4981 aaacggcgcc cgcttgagcg gacgccgtct tatttttact ttttttcgga taaaagcctt
     5041 tccaccccgg ccagtttgac gcagaggcaa agcagctcga tggccgtttt aagttccccg
     5101 acaggcggat aagtcggcgc gatccggata ttcctgtctc tcggatcttt gccataaggg
     5161 aatgtagctc cggccggggt cagcttgaca cccgcctcgc cgcaaagcct cacggtctcc
     5221 cgcgcgcagc cgtcgggtgt gttcaggctg atgaaatacc cgccgttggg ttgggtccat
     5281 tcggcaagcc ctgttccgcc gagttctttt tcgagccctt ccgttaccgc tttaaatttc
     5341 ggccgaagga tcgcagcgtg ctttttcatg tgatttcgga taccttcgat atccccaaaa
     5401 taacggacat gccgaagctg attgatctta tccggaccga tagcctgagc agccatctgc
     5461 tttttaatga aggcaacatt atcacacgaa gccgccatca tcgcgactcc cgaaccgggg
     5521 aaactgattt tggaggttga agcaaaaagg aataccatgt cttctctgcc gtttttcctg
     5581 caggcttcca aaatgttttt cagagggtcg gacgtttccc caagatcatg aacgcaatag
     5641 gcgttatccc aaaaaatcct gaaatccggg gctttgggct tcagggctgc gaaacgatcg
     5701 accaccgcgt cggaatacgt aacgccttcg gggttggaat atttgggcac gcaccagatt
     5761 cccttgacag tttcatcgct ttccaccagc cgcttgacag catccatatc cggcccgtcg
     5821 cttctcatct caatcggaat catctcgatg ccgaaatgct cgcagattgc gaaatgccgg
     5881 tcataaccgg ggctcggaca aagaaaccga attttatcct gccggcacca gggctcttca
     5941 ctatggagca caccgtgggt gtacgctctg gcgactgtat catacatcat agtcaggctg
     6001 gagttcccgc caacgataat ttccgtcgga ttaacctcca gcaattgcgc aaacaacgcc
     6061 tttgcttccg gaataccgtc cagcaggccg tagttcctgc agtcggttcc gtcctgcgca
     6121 cggcagggtt ggctcgaatt gatgcagtcg agaatcccca tcgaaagatt cagctgatcg
     6181 gctcccggtt tgccccttga catgtcaagg gccagatttt tctgctgaaa tcctctgtat
     6241 tttttttcga gatccgcttt ttcagattcc agttctcgct ttgtcagaga cgcatattcc
     6301 gacataaaaa agcctccatt atcacaagtt taccaattga ccaaagcgct tcagccgctt
     6361 gtccggcagg gcacaccggc gtcccgctgc aactcacagg cgtcccgcat tcaacattca
     6421 ttaaaattgt agaactcaac cttaatattc tattctattc aaaacatttt tgcaagatca
     6481 cccatatcaa tatgcaaaat ataaaagatg gcacaaaacc atcctccatc gaacggcaat
     6541 tttatcaata gcggctaaca accgggccga cccgcctgct ctatggccga tcgggtcact
     6601 tctccggcag tcacaccgta ttctctggcc gcccgctgaa ccgactcaaa ttccggtttc
     6661 atcttaacaa tatcgccgta tcggcttatt ttaacccgta taggcccata agcggacggc
     6721 acttccctga aatcccgggc cattgtgatt ctttgcgccg tccggattct cagaccgatt
     6781 gagcctgtct gccggaaaac aatcccgaca atttcatcga ccagacggct ttcacacaaa
     6841 acggtcagca gataagccgg ccggtttttc ttcatataaa tcggggtata ataggcgtct
     6901 gccgccccgg cttcaagcag agctgcgaca caggctccaa gttcttcccc ggtactgtca
     6961 tcaatgcagg tctcgagcac ttcaatttca tccgctccgc cattccgttc gtccgtttcc
     7021 ccaagaaatg ccctaagtat gttcgccttt tcaaaatctt tttttccgca gccggttccg
     7081 atcttttgca gggctattcg gggcatccgc ccaaaaccgt cactcaccgc ggcggcaatt
     7141 gcagcgccgg tcggggtcac catttcaccc atttgctccg acacttcaaa cgggatgccc
     7201 gcgcgagcca taatctccgc agtggccggc gccggaaccg gaagaatccc atgggcgcag
     7261 cggacaaatc cgctgccttc gacaaacgga gaaaacagaa tccggtccgg cttgatctgc
     7321 tcgatacaga cggccgcacc aacaatatcc gctatcgaat cggcggcccc cacctcatga
     7381 aaatgcacct ccgagcggtc aattccgtga accttgcctt ctgcgtcggc caccaccgcg
     7441 acaatctttt ttgacagtgt cttgacgaag ttggaaagcg cgctgtcatc aatgatcttt
     7501 tctacatcgg aaaggtgacg atggacatgc cccgcctcct cctttataac gacatcaaaa
     7561 tcggtgcagg caatcccgca tttctggctt tttgtgattt ttatttcgaa ttcgccgagt
     7621 ccgaggccca tcagggactg ccgaagcacc cgggcatcaa cgccgagatc cagcatggca
     7681 gccacaaaca tgtctccgct gatacccgat ccgcactgga aataaatcgt cttcatccat
     7741 ccaccctctc atgttcctca tctgccgcga gccgattgat ctgggcggca agacagcccg
     7801 cgccgaaccc attatcaatg ttgacgacac cgatccccgc cgagcaggaa ttgagcatgg
     7861 taagcagcgc tgaaagaccg ccgaaattgg agccatagcc cacgctcgtc ggaacggcaa
     7921 ccacgggcct gtccaccagg cctccgatta cgcttgccag cgccccttcc attccggcca
     7981 ccgcaatcac cacattagcg cttcgaattt cttcgagcct gtcaaaaagc cggtggattc
     8041 cggccacgcc aacgtcaaaa atcctctccg ccctgcagcc aaatgcctcg gctgtcaccg
     8101 cggcctcttc cgcgacgggt atatcggcgg ttcctgccgt gcagacggct acttttccga
     8161 ttttacgagg gcttatcagg gttaccgtaa ccgcacgagc ctgctcgtgg taaaccgcat
     8221 ccggcaggac tgagcggatt gcttcatacg cggtgcgatc cgctcgggtc gccaggacat
     8281 tggatcccct cgccgccaga tcctgcgcga ttctgccggt ctgctccgcc gttttcccct
     8341 ggcagaagat tacttccggg aatccgctcc gaatctgccg gtggtggtcg accctcgcaa
     8401 atcccagatt ttcgtagggg agtattttca gcttctcata ggcctcttcc ggctttattt
     8461 ccccgtccgc aaccttttca agcagcgatt taatatccat attgtcctcc ttcggctggt
     8521 ttcgcctccg attaaaattc cgcgcttccc ggcgttctgg ggaagggaag aacatctcgg
     8581 atgttggtga tccccgtaat atacatgatc agccgctcaa agcccaggcc aaacccgcag
     8641 tgttccaccc cgccgaaccg gcgcaggtca agataccacc catagtcctc tggtttcaga
     8701 tccagctcct tcatccttga gagcagaacg tccagccgtt cttccctttg gga
//
LOCUS       DATALY010000189         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_386120,
            whole genome shotgun sequence.
ACCESSION   DATALY010000189 DATALY010000000
VERSION     DATALY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386120"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..272
                     /locus_tag="VHR42_05560"
     CDS             120..272
                     /locus_tag="VHR42_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026676.1"
                     /translation="MKAITMNIVANLLGLGNAATPLGLRAMKEMSAGSKDKKNGNGFH
                     GYLRSD"
     gene            250..441
                     /locus_tag="VHR42_05565"
     CDS             250..441
                     /locus_tag="VHR42_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026677.1"
                     /translation="MVTFVVINTASIQLIPTTIAAIRIKYGSASPFDIMPAMWFASAV
                     TLCFGVLLAKVLSGSRKKN"
     gene            467..997
                     /locus_tag="VHR42_05570"
     CDS             467..997
                     /locus_tag="VHR42_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX3026678.1"
                     /translation="MIQISRIGSYVVPFVILVIVLAGLLKKENVFDLFLNGAKEGIST
                     AVGILPALIALMTGIAMFRASGAFDLIVAGLSPLGKLLHFPSEVVPLALMRPISGSGS
                     LSILEYLLKTYGPDSFQGRVASVLQGSTETTFYTIAVYYGSVNVSKTRHTVPSALTAE
                     FVGFIMSILSVRLFLG"
     gene            complement(1017..1361)
                     /locus_tag="VHR42_05575"
     CDS             complement(1017..1361)
                     /locus_tag="VHR42_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX3026679.1"
                     /translation="MNGFGSGQYVTRGVTLMSEGMGVIGIILGSLLIILVTALIVLTV
                     LHIIRLVSHKGCPPVPPGMPVNCPPNRLGNIQGTASADLGAAVSVLNTRLAKGEISQE
                     EYIKLKDQLFHP"
     gene            complement(1679..>2529)
                     /gene="gpmI"
                     /locus_tag="VHR42_05580"
     CDS             complement(1679..>2529)
                     /gene="gpmI"
                     /locus_tag="VHR42_05580"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX3026680.1"
                     /translation="KENVTDEFVVPAVCAKDARIQKGDSVIFFNFRPDRARELTRTIV
                     DPDFAGFDRKKGFFPVFYVCMTQYDATMPNVQVAFKPQSLANTFGEYISSLHLKQLRI
                     AETEKYAHVTFFFNGGVEKESEGEDRILVASPKVATYDLKPEMSAYEVTDKVVGAINS
                     AKYDAIVLNFANCDMVGHTGVFEAAVKAVETVDACIGRVTQAVAAQGGVSLITADHGN
                     ADQMKEPDGSPFTAHTTNRVPFIVSGYDCKLRPEGKLADIAPTILDIMELPQPEEMSG
                     VSLIRK"
BASE COUNT          595 a          654 c          595 g          685 t
ORIGIN      
        1 catgaacggt tttggctcgg gacaatatgt cacaagaggg gttacactga tgtcggaggg
       61 aatgggcgtt atcggtatta ttctgggatc gcttttaatc atccttggac ggcgcggcaa
      121 tgaaagccat tacaatgaat attgtagcaa atcttttggg tcttggcaat gcggcgactc
      181 ctctcggcct gcgcgccatg aaggaaatgt ccgccggatc aaaggacaaa aaaaacggca
      241 acggattcca tggttacctt cgtagtgatt aacacagcct ccattcagct gattcccact
      301 accatagcgg cgatccgtat aaagtacggt tcggcgtctc cgtttgatat catgccggcg
      361 atgtggtttg cttccgccgt tacgctttgt ttcggcgtcc tgctcgcaaa agtgctgtca
      421 ggctcccgta aaaaaaattg aaataacggc tttcagccct taggcggtga ttcagatctc
      481 ccggatcggc agttatgtag ttccttttgt tatacttgta attgttttag caggcttact
      541 gaaaaaagaa aatgtatttg acctgtttct gaacggcgca aaagaaggga tttccaccgc
      601 cgtaggaatc ctccccgctt taattgcgct gatgacggga attgccatgt tccgcgcttc
      661 cggcgccttt gacctgattg tcgcgggact ttctcctctt ggaaaactgc tgcattttcc
      721 aagcgaggtt gttccccttg ctctgatgcg tcccatttcc ggaagcggct cgctttccat
      781 actcgagtat ctgctgaaaa cctacggccc cgattccttt cagggccgcg tggcttccgt
      841 tctgcagggg tcaactgaaa ccacgtttta tacgatcgct gtctattatg gctccgtaaa
      901 cgtttctaaa acccgccaca cggtgccttc cgccctgacg gcggagtttg tcggttttat
      961 tatgagtatc ctttccgtca ggcttttcct tggctgagcg ctgtttttcg cgcccctcag
     1021 ggatggaaaa gctgatcctt cagttttata tactcttcct gcgatatttc gccttttgcc
     1081 aaccgcgtat tcagaactga aacggcggcg ccaagatcgg cggaagctgt cccctgaata
     1141 tttcctaacc tgttcggcgg gcagtttacc ggcattccgg gcggaaccgg cggacaacct
     1201 ttatgcgaaa cgagccggat aatatgcaga accgtcaaaa caatcagcgc cgtcacaagg
     1261 atgattaaaa gcgatcccag aataataccg ataacgccca ttccctccga catcagtgta
     1321 acccctcttg tgacatattg tcccgagcca aaaccgttca tgttaacctc cttctcgccg
     1381 catgagcggc gacataaata ggattaaaaa ggaactgtgc cgcgcagcgg atgccggtca
     1441 tgctttcgaa cggcaagcgc cttttccgag cgtgaaaata ttttcacgca tcactccttt
     1501 taaatactct tgtgccggca aaagtcttta cttgccgaca atttttttct ttttggtttt
     1561 ttatatttta attttaatca ataaaaatga ataaaaagtt tagcaaattt aaacaatcta
     1621 taaactaaag ccggctcttc aaaagaagag ccggctttag tttggtgtat tccccctgtt
     1681 atttccggat cagggaaaca ccgctcattt cctcgggctg cggaagctcc ataatatcaa
     1741 gaatggtcgg agcgatatcc gcaagcttgc cttccggacg aagcttgcag tcgtaacccg
     1801 aaacgataaa cggaacccgg ttggtcgtat gggctgtaaa cggagaaccg tccggttcct
     1861 tcatctgatc cgcattcccg tggtcggccg taatcaggga aacgcctccc tgcgccgcga
     1921 cggcttgagt aactctgccg atgcaggcgt caaccgtttc cacggccttt accgcggcct
     1981 cgaatacccc tgtatggcca accatgtcgc agttggcaaa attgagcacg atcgcgtcat
     2041 attttgcaga attgatcgct cccactactt tatcggtgac ttcgtaagcg ctcatttccg
     2101 gttttaaatc ataagtggca acttttggcg aagcaacgag aattctgtcc tcgccttccg
     2161 attccttttc cacgccgccg ttaaaaaaga atgtaacatg agcgtacttc tcggtttcgg
     2221 cgattctgag ctgttttaaa tgcaggctac tgatatactc gccgaaggtg tttgcaagag
     2281 actgcggttt aaacgcaacc tgcacattgg gcattgtggc gtcatactgc gtcatgcaga
     2341 cataaaatac cgggaagaag ccctttttcc ggtcaaatcc ggcaaaatcc gggtcaacga
     2401 tggttctcgt gagctcccgg gccctgtcgg gacggaaatt gaagaaaatt acggaatcgc
     2461 ccttctgaat ccgcgcgtct ttggcacaga ccgccggtac gacaaattcg tcggtaacat
     2521 tctctttgt
//
LOCUS       DATALY010000190         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_473860,
            whole genome shotgun sequence.
ACCESSION   DATALY010000190 DATALY010000000
VERSION     DATALY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473860"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..450
                     /locus_tag="VHR42_05585"
     CDS             79..450
                     /locus_tag="VHR42_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3026681.1"
                     /translation="MEFFLESAGQGFYSAESVESLPSVWKLNYFDKKNGGYVITDKIR
                     DEVIFRVFNLMDDRFPFKKKFHVILVKCLSYGCSFSKMESVNTCPVCRCRLSGWMQAN
                     RLSSFPKIAPYQEAFHSLSEV"
     gene            complement(401..874)
                     /locus_tag="VHR42_05590"
     CDS             complement(401..874)
                     /locus_tag="VHR42_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3026682.1"
                     /translation="MAELKENVTTLEEDTQHGKYLTFLLGEDVFGIEIKYVTEIIGIQ
                     PITPVPNVPPFVKGIINLRGKIIPVIDVRVKFGKETVEYDDRTCIIVLETGEIAVGFI
                     VDNVAEVLNISDENIVPPPDYRTGYQNKYIKGIGKAGDEVKLLIDCEKLLDTEQF"
     gene            complement(948..1862)
                     /locus_tag="VHR42_05595"
     CDS             complement(948..1862)
                     /locus_tag="VHR42_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX3026683.1"
                     /translation="MLSGREAIIIKKQQVEKDKSERWMLTYSDLMNLLLILFIILYSS
                     SRIDAAKAQQIANSIKKGFNTTDMKVGTTPAFAASSGKKTASTPTYKTGDYDYNRLAE
                     ALINLVKQKGLQNQVVVQVSSKGIIISLSDAVLFAKGKADIKSDYTNLLVQIGNLLKC
                     VDFSQLVVEGFTDSDPIHTAQFEDNRDLSSKRANNVARLLQYQCGIAADKISSLGYGE
                     YRPFAPNDTEENKSKNRRVVITILKGSVDVDAVIEANNLVSAYNTLTTSQAAQSSQSP
                     QSSKTSSAASSHKVSSAASAKIRASSTG"
     gene            complement(1876..2667)
                     /locus_tag="VHR42_05600"
     CDS             complement(1876..2667)
                     /locus_tag="VHR42_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3026684.1"
                     /translation="MNFSLILGVLVALGGVLAGFLIEKGTFISLWSPSAFCIVFGGTI
                     GIAMITYPMSSMKKIPAAMKLITFQKKHDTKKLVDMLCDIANKARKEGILSLESEADK
                     VKDPFIKKGLGYIADGVDPEFLRKMLENEIEVVEKKYKAAAAVFEGMGGTGPTMGVLG
                     TVMGMTTILKSMNADMDSLGKNIAVAFLATLYGVGSANILWLPIGGNIKVVAERESDY
                     QMVILEGLMAIQSGESSSRLRDRLETMLSEKKGKSAGKEEHEEEK"
BASE COUNT          663 a          668 c          598 g          836 t
ORIGIN      
        1 ccggcaaacc ggacacgtat ttacggattc cattttagaa aaactgcagc catacgacaa
       61 acattttact aatattacgt ggaatttttt cttgaatcgg ccggccaggg gttttacagt
      121 gccgaaagcg tcgaatcttt accatccgtt tggaaactga attattttga caaaaaaaac
      181 ggcggatacg ttattaccga caaaatacgg gatgaggtta tttttagggt tttcaacctg
      241 atggacgacc ggtttccatt caagaaaaaa ttccacgtaa tattagtaaa atgtttgtcg
      301 tatggctgca gtttttctaa aatggaatcc gtaaatacgt gtccggtttg ccggtgtcgc
      361 ttgtccgggt ggatgcaggc caacaggctt tcaagttttc ctaaaattgc tccgtatcaa
      421 gaagcttttc acagtctatc agaagtttaa cctcatcgcc ggctttgccg atgcccttaa
      481 tatatttgtt ttgatatccg gtcctgtaat ccggaggagg aacgatgttt tcatcggaaa
      541 tattcaatac ttccgctacg ttgtcaacga taaaccctac cgctatttca ccggtttcca
      601 gaacgataat acaggtccgg tcgtcgtatt ccacggtttc ttttccgaat tttacacgca
      661 cgtcgatcac cggaatgatt ttccctctga ggtttatgat tccttttaca aaaggcggca
      721 cgttcggaac cggggtgatc ggctgtatcc cgattatttc cgtaacatac tttatttcga
      781 tcccgaaaac atcttccccc agtaaaaatg tcagatattt tccgtgctgc gtgtcttctt
      841 caagagtcgt gacgttctct ttcagttcag ccattgcgca acttcctttc ttttttataa
      901 ataggatgtt cgccttatgt tttattaaat ataaaatatc gatgaattta tccggtagaa
      961 gacgccctga tttttgcaga cgccgccgag gaaactttat gagaagatgc agcggaagaa
     1021 gtcttggaac tctgcgggct ttgtgagctt tgcgcagcct ggctggttgt cagggtgtta
     1081 tacgcggaca ccagattatt tgcctcaatt accgcatcga catcaacgct tcctttgagt
     1141 atcgttatga cgaccctgcg gtttttcgac ttgttttctt ctgtgtcgtt tggcgcgaac
     1201 ggcctgtatt caccgtaacc aagggaggat attttgtctg ctgctatgcc gcattgatac
     1261 tgcagcagcc ttgcaacgtt attcgccctt ttggagctga ggtcacggtt gtcctcaaac
     1321 tgggcggtat ggatcgggtc ggaatccgta aagccttcca cgacaagctg ggaaaaatcc
     1381 acgcatttca gaaggttgcc tatttgtacg agcagattgg tataatcgct tttaatatcc
     1441 gctttgccct ttgcgaaaag cacggcatcg ctcagtgaaa tgataatgcc cttgctgctt
     1501 acctgcacca caacctggtt ttgaagccct ttttgcttga caaggttaat aagcgcttcg
     1561 gccagcctgt tataatcata atcaccggtt ttgtaggtcg gggttgaagc ggtttttttg
     1621 ccgctggaag ccgcaaaggc cggtgtggtt ccaaccttca tatcggtcgt gttaaagccc
     1681 tttttgatcg agttcgcgat ttgctgcgcc tttgccgcgt caatcctgct gctgctgtat
     1741 aatataataa acaaaatcag cagaaggttc atcagatcgg aatatgtaag catccagcgc
     1801 tcgcttttgt ctttttcaac ttgctgcttt tttataatga tggcctcccg tcctgacagt
     1861 atctattttc gttaattatt tttcttcctc atgttcttct ttaccggctg atttgccttt
     1921 tttctcagaa agcattgtct cgagccggtc cctcagtctg gaggaagatt cgccggactg
     1981 aatcgccata agcccttcca gtataaccat ctgataatcg ctttcccgtt ccgcgacaac
     2041 ctttatgttg ccgccgatcg gaagccacag aatattggcc gaaccgacgc cgtacagtgt
     2101 tgccagaaat gccaccgcga tatttttccc aaggctgtcc atatcggcat tcatgctttt
     2161 caggatggtc gtcatgccca tgacggtacc cagaacgccc attgtcggcc ccgtaccgcc
     2221 catgccttcg aaaacagccg cggccgcttt atattttttt tcgacaacct cgatctcgtt
     2281 ttcaagcatt ttccgtagga actccgggtc gacgccgtcg gcgatatagc caagcccttt
     2341 cttgataaac gggtctttta ccttgtcggc ttcggattcc agcgagagga ttccttcttt
     2401 tctggccttg tttgcaatgt cgcaaagcat atccacaagc tttttggtat catgcttttt
     2461 ctggaatgtt atcagtttca ttgcagccgg tattttcttc atgctgctca tcggataggt
     2521 aatcatcgca attccgatcg tgccgccgaa aacaatgcag aacgccgaag gagaccaaag
     2581 ggaaataaac gttccttttt cgatcaggaa accggccaaa acgcctccga gagctaccaa
     2641 tacgcctaaa atcagtgaaa aattcataag aacccccgtc tcgccatcac agcaaaaaaa
     2701 gaaaacaaat taaataagtg cgcaggaatg atcctgctct gtatggcggt cgccaatttt
     2761 tatat
//
LOCUS       DATALY010000191         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_41547,
            whole genome shotgun sequence.
ACCESSION   DATALY010000191 DATALY010000000
VERSION     DATALY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41547"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VHR42_05605"
     CDS             <1..606
                     /locus_tag="VHR42_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3026685.1"
                     /translation="FKNERTRPVHDLIGHIDLDAPGRILDIGCGPGNSSAVLAERYPG
                     SEIIGLDSSPAMIERAKKECLKARFLTEDGSKDLSHLGKFDLIFANASLQWMPDHARL
                     LRGYVKMLQKGGAIAMQIPEFLQMPAAKILAGAAASLQFAGFFRDFDPGLHENPPRIL
                     LQRAKPRQHASQPVGDLLLPRSARLRRNHGVALIDRDEALP"
     gene            830..1276
                     /locus_tag="VHR42_05610"
     CDS             830..1276
                     /locus_tag="VHR42_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX3026686.1"
                     /translation="MSKIDLVRQEMMKALKEKDMERKNALSILLSALKAKFIDKRAEL
                     TEDEENAIVFREIKQAQETLASAAQGRADLAEECNLRIKIYSEFVPKMMNETEISTII
                     QKVLGELKIDHPEAKDKGKIMKTLMPLVKGKADGALVNRLVGKLFQ"
     gene            complement(1380..>1808)
                     /locus_tag="VHR42_05615"
     CDS             complement(1380..>1808)
                     /locus_tag="VHR42_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HEX3026687.1"
                     /translation="LFARQKGDLAQMVQGIRKIENLKPGGRVLIAEGCTHHRQSDDIG
                     KVKIPRWIRQIVGGDIQFEWASGACFPRDIDRFDAVVHCGGCMINRREMQYRMDYARQ
                     NGVGITNYGMLIAYVQGILPRALRPFPAAALALEENRIDI"
BASE COUNT          461 a          489 c          458 g          400 t
ORIGIN      
        1 tttaaaaatg aacgaaccag gcccgttcat gacctgatcg ggcatattga tcttgatgcg
       61 ccggggagaa ttctcgacat cgggtgcggc cccggaaaca gctccgccgt cctggccgag
      121 cgttatcccg gttccgaaat aatcgggctg gattcctccc ccgccatgat cgaacgcgcg
      181 aaaaaagagt gcctgaaagc tcgttttctt acagaggacg gaagcaagga tttatcacac
      241 cttggtaaat tcgatctgat tttcgcaaac gcctctctgc aatggatgcc ggatcacgcc
      301 cggttgctgc ggggctatgt taaaatgctg caaaaaggcg gggcaatcgc catgcagata
      361 cctgagtttt tacagatgcc ggcagcaaaa atcttagccg gcgcggcggc ttccttgcaa
      421 tttgccgggt ttttcagaga cttcgacccg gggctgcatg agaatccccc ccgaatatta
      481 ctacaacgcg ctaagccgcg gcagcacgca agtcaacctg tgggagacct attattacca
      541 cgttctgccc gactgcgccg caatcacgga gtggctctca tcgaccggga tgaagcccta
      601 ccttgaccgg cttccggagc aatcgcgccc cgaatttgtc cggcaggttc aggccggttt
      661 taacgcgaac tatcccatgc aggacgacgg ccgggttctg tttattttcc ggcggctgtt
      721 ttttattgct tattgttagt tgaatttcat gcggcatcgc attttcaact gatcaaaatt
      781 atgagggccc cgaatgatgc ccccggatac aacaggagga aaaaacaaga tgtcaaaaat
      841 cgatctggta agacaggaaa tgatgaaggc gctgaaagaa aaggacatgg agcgtaagaa
      901 cgccctgtcg attctgcttt ccgctctgaa ggccaagttt atcgacaaac gcgccgagct
      961 gaccgaagac gaggaaaacg cgatcgtttt cagggaaatc aaacaggccc aggaaacgct
     1021 ggcttccgcc gcgcagggcc gagcggatct cgccgaagaa tgcaatctca gaatcaaaat
     1081 ttattccgaa ttcgttccga aaatgatgaa tgagactgaa atcagcacga tcattcaaaa
     1141 agtactcggc gagcttaaaa tcgaccatcc cgaggcgaaa gacaagggaa agatcatgaa
     1201 aaccctgatg cctcttgtca agggcaaggc ggacggcgca ttggtcaacc gtcttgtggg
     1261 gaaactgttt cagtagttca taacgctgta attccgatca aagggctgcc tctccatgac
     1321 tttaaatggt catagaaagg cagccccttc attttataaa aagctttaaa attacaagat
     1381 tatatatcga tccggttttc ttccagggca agcgccgcgg cggggaacgg ccgcagcgcc
     1441 cggggcagga ttccctgcac ataggcgatc agcataccgt aattggtgat tccgacgccg
     1501 ttttgccggg cataatccat ccgatactgc atttcccgcc ggttgatcat acacccgccg
     1561 caatggacaa ccgcgtcaaa ccggtcgatg tcccggggaa aacaagcccc cgaggcccat
     1621 tcaaactgaa tgtctccgcc cacgatctgg cgaatccatc tcggaatctt cacctttccg
     1681 atatcatccg actgccggtg atgggtgcag ccttcggcaa tcagaaccct gccgccgggt
     1741 ttcaaattct cgattttcct gataccctgc accatctggg ccagatcgcc tttctgccgt
     1801 gcaaaaag
//
LOCUS       DATALY010000192         5284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_558574,
            whole genome shotgun sequence.
ACCESSION   DATALY010000192 DATALY010000000
VERSION     DATALY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558574"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VHR42_05620"
     CDS             <1..311
                     /locus_tag="VHR42_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026688.1"
                     /translation="VNILNYKGVILYETVSKSGSKYIHGYLPAKTGSIVFKYQYGYTS
                     VNRQPVYSLSGKRTASYLNARQKATMLYMVGKKCAVLFDTKSGKNTGFGVVNFRGKAI
                     "
     gene            complement(428..1792)
                     /locus_tag="VHR42_05625"
     CDS             complement(428..1792)
                     /locus_tag="VHR42_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3026689.1"
                     /translation="MALKKQFIKFIIPSVVSMWVFSIYSMIDGIFVAKGVGETALAAV
                     NLSIPYISLIFALALLFAVGTSTLAAVYSGQGDHDQASRIFSLNTAVLLIVSVLIMIF
                     SSVFLDSIISLLGATDITRSYVKDYLGVIILFVPFYMLSYSLEVIIKTDGFPVLATLG
                     VLVSILVNGFLDYLFILVFHLGIRGAGLAAGCAQLSLFLLFFIHFLKKKGYLRFTRFR
                     WNLSVYHRILPIGVSDFITELSNGMIIFLFNHVILSVVGDSGVVGYTVVSYVNSLVLM
                     TMMGITQGMQPLSSFYYGQREHGVCQRLLRYSVLCIAVCSLVAFAVGMLLPEPLVGIF
                     IQRQHTELFAYSVRALRLFSFSFLIMGFNVVIAGFFVSIERPAYSMPISISRGLILIS
                     ASLLVLSALFGETGIWLSTLVSESLCLCLTLALFFKYRSDHKAQIRKAPSAPSKTCID
                     APRM"
     gene            1883..2701
                     /locus_tag="VHR42_05630"
     CDS             1883..2701
                     /locus_tag="VHR42_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3026690.1"
                     /translation="MQEYYKIGEISKLYGICADSLRYYEEIGVLSPKRDENGYRLYSI
                     TDIWRLNVIKDLRRLGFSMQHIKDYLDNRSISSTLRLLEEEDVLIKEQIEGLMRIQRN
                     IAKKVSLIQKGLGIKEGEFSIRKFPERKCFQIKENIKRDEDIDLLKKKLQSSHEDKLY
                     ILGNSCIGSRVPLEYIRKQQYHHYNSVFVIDESSGDSDFILESGEYATVSYRGDYIKN
                     ADYVPRLVQYAGEKGYRLMGDLLELLLIDIHETADSNEFSTELQIRVERESFSD"
     gene            2841..3590
                     /locus_tag="VHR42_05635"
     CDS             2841..3590
                     /locus_tag="VHR42_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEX3026691.1"
                     /translation="MFYRAPGSDEKSQKRHAIHESVPFGVLLAVVGGFLDAYTFIGRG
                     GVFANAQTGNIVLVGIYVSRGELQKSLGSLVPIFAFVMGVLVAESIKRNASRLWALDC
                     ARTVLFFEMTVLFMIGFVPSTVPNYFVTVTVSFVASLQVSTFRKLVDSPYSTTMCTGN
                     LRTAAREAYLAVTQKDRESARRSLRFFIIIFSFFVGAVSGGLLTSAVKEKAVWVAVAC
                     LAAAAVLFSIANQNPAGGQKNSAGRPERKNL"
     gene            3679..4698
                     /locus_tag="VHR42_05640"
     CDS             3679..4698
                     /locus_tag="VHR42_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3026692.1"
                     /translation="MDQMRLGKTEIMASRYGFGALPIQRVSREEAALLLRKAFDHGVN
                     FFDTARAYTDSEEKMGYALSDVRSHIYIATKTHAKDKKTLFEHLELSLKKLQTDYIDV
                     YQLHNPAALDYENPDGVLAGLAEAKRKGLVRFIGLTSHRLSLAMKAAASGRIDTIQFP
                     LSMLSSSEDIGLTEECRKYDVGLIAMKAMSGGLIRHPEAAFAFLRQYANVLPIWGVQR
                     EEELDEFLGYEKNPPALDDRMKQLIEDERRELSGDFCRGCGYCQPCAAGIPIETAARL
                     TLLMSRAPYEQFLTDDFREQMDRIENCTGCGHCRSHCPYGLDTPALLRRNLEDYRAFR
                     MKHGC"
     gene            complement(4844..>5284)
                     /locus_tag="VHR42_05645"
     CDS             complement(4844..>5284)
                     /locus_tag="VHR42_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX3026693.1"
                     /translation="ARARQLQGTSGSGNSGGGSTGGSSVSGNKIVGYAMGFVGCPYVS
                     GGISPSGFDCSGFTQYVYAYAGKYLPHDAAAQSGYGTAVSKGNLQPGDLVFFATSGGG
                     INHVGIYVGGGNFIAANTGHAYRVQVQPLFSGYWAARYVCARHI"
BASE COUNT         1465 a         1162 c         1373 g         1284 t
ORIGIN      
        1 tcgttaacat tctaaattac aaaggcgtta ttctgtatga gactgtttca aaatccggaa
       61 gtaaatatat tcacggatat cttccggcta aaacgggtag tattgttttt aaatatcagt
      121 acggttatac gagtgtcaac agacagccgg tgtattccct gagcggaaaa agaacagcgt
      181 cttatcttaa cgcccggcaa aaggcgacaa tgctttacat ggtcggtaaa aagtgcgccg
      241 ttttatttga cacaaaaagc gggaaaaata ccggttttgg cgtcgtcaat ttcagaggaa
      301 aagctatctg actgagaata gaataaaatc atcgaaaaaa cgccgaagat tttgattctt
      361 cggcgttttt tgatgatagg ctgtgatgga tgaaagtgag cgcccggcag cgcccgggtt
      421 ttttcgatta catacgtggc gcgtcgatac aggtttttga cggggcgctt ggagcctttc
      481 tgatttgcgc tttatggtcg gaacgatact tgaaaaacaa tgccagagtc agacacagac
      541 agagcgattc ggagaccagt gtagagagcc aaatgcccgt ttcaccaaag agtgcggata
      601 ggacgagcaa acttgcgctg atcaatataa gacccctgct gatcgagatg ggcatcgagt
      661 aggcgggacg ctcaatggag acaaagaaac cggctattac gacgttaaag cccataatca
      721 ggaaggaaaa gctgaacaga cgcaatgccc ttacggaata tgcaaataat tcagtatgct
      781 gtctctggat aaaaatgccg actaatggct ccgggagcag cattccgacg gcaaaggcga
      841 cgagggagca aacggcgata cacagtacgg aatagcgcag cagcctttgg cagacgccat
      901 gttccctctg tccgtagtaa aagctcgaca gcggctgcat cccctgcgtg attcccatca
      961 tcgtcattaa taccagagaa ttcacgtagg aaacaaccgt ataaccgacg acgcctgagt
     1021 ccccgacgac ggaaagaatg acgtggttaa aaagaaatat gatcatacca tttgatagct
     1081 cagtgatgaa atcggaaacg ccgatgggca ggatacggtg gtaaacggag agattccagc
     1141 ggaaacgggt aaagcgcagg tatcccttct ttttcagaaa atggataaaa aacagcagga
     1201 aaagggaaag ctgcgcgcag ccggcggcaa gtcccgcacc tcgtatcccc aaatgaaaga
     1261 ccaaaataaa aaggtagtca agaaagccgt tgaccaaaat actgacgaga acacccagcg
     1321 tcgcgagtac agggaaaccg tctgttttaa tgataacctc cagcgagtag gaaagcatat
     1381 agaaaggaac gaacagaata atgacgccga gataatcctt tacatagctc cttgtaatat
     1441 cggtggcccc cagcaggctg atgatggaat ccagaaatac agaactgaat atcatgatta
     1501 aaacggagac gatcagtaaa acagccgtat tcagcgagaa gatgcggctt gcctgatcgt
     1561 ggtcgccttg cccggaataa accgcagcaa gagtcgacgt tcccacagcg aacagcagcg
     1621 ccagcgcgaa gattaagcta atgtatggaa ttgacagatt aaccgcggcc agcgccgttt
     1681 caccgacgcc ctttgcaacg aatatgccgt cgatcatact gtaaatcgaa aacacccaca
     1741 tcgaaacgac agacggaatg atgaatttga taaattgttt tttgagagcc atggtaaaac
     1801 ctcctgttga ttattattct aaaccttggt atcatactaa ggtcaagaca attttttgat
     1861 catttttggg aggcggtgta agatgcagga atattataaa atcggtgaaa tttcaaaact
     1921 gtatggaatc tgtgcggatt ctctccgcta ttatgaagaa atcggcgttc tgtcacccaa
     1981 aagggatgag aacggatacc ggctttacag tattaccgat atctggagac tgaatgtgat
     2041 taaagatttg cgcagacttg gcttttccat gcagcatatt aaggattatc tggataaccg
     2101 aagcatttcc tctacgctcc ggctccttga ggaagaagac gttctgatca aggaacaaat
     2161 tgaggggctt atgcggattc agcgaaatat tgcaaagaag gtttcgctga ttcagaaggg
     2221 tctcgggatc aaagaagggg agttttcaat tcggaaattt ccggaacgca aatgctttca
     2281 gataaaagag aatattaaac gggatgaaga catcgatttg ttaaagaaaa agctgcaaag
     2341 cagtcatgag gataaactct atattcttgg aaacagctgc atcggctccc gggtcccgtt
     2401 ggaatatatc agaaaacagc agtaccatca ttataattct gttttcgtca ttgacgaatc
     2461 cagcggtgac agtgatttta ttcttgaaag cggagaatac gcaacggttt cctaccgggg
     2521 agattacatt aaaaacgcgg attacgttcc cagactggtt cagtacgcag gggagaaggg
     2581 ataccgtttg atgggcgacc tgctggaact tctcctgatt gatattcatg aaacagccga
     2641 ttcaaacgaa ttttccacag agctgcagat aagggtggaa agggagagct tttctgatta
     2701 aaaggaaaga aacgtcccgc ttttgcggga cgtttctttg tggatggtga cggccgggtt
     2761 gccagttcga agagagggtg ataaaatatc ttattgaagt attttgagtt gaaaaggggg
     2821 cctttctttt gaaaggcggt ttgttttata gagcgccggg ttctgatgaa aagtcccaaa
     2881 aaagacatgc gattcatgaa tcggttccgt tcggggtttt gctcgcggtt gtcggcggct
     2941 ttttagatgc ttatacattt ataggcaggg gcggcgtgtt tgcaaacgcc cagaccggta
     3001 atatcgtcct ggtgggaatt tatgtgtccc gtggtgaatt gcagaaatcg ctgggcagtc
     3061 tggtgccgat cttcgccttt gttatggggg tattggtcgc tgagtcgatt aaaaggaacg
     3121 cctcgcgtct ttgggcgttg gactgtgccc gaaccgttct tttctttgaa atgaccgtgc
     3181 tgtttatgat cggatttgtg ccgtcgactg ttcccaacta ttttgtgacc gtcaccgttt
     3241 cgtttgttgc gtcgcttcag gtatccactt tccgaaaact tgtggattcc ccgtacagca
     3301 cgaccatgtg taccggaaat ctgcgaacgg ccgcccgtga ggcttacctt gccgtaacac
     3361 aaaaggatcg ggagtcggca agacggtcgc tccgcttttt tatcatcatt ttttcctttt
     3421 tcgtcggtgc cgtatcaggc gggttactga cttccgccgt gaaggagaag gcggtttggg
     3481 tcgcggttgc ttgtttggcc gccgccgcgg tgttgttcag tatagccaat cagaatcctg
     3541 ccggcgggca aaaaaattct gcaggccgcc ctgagcgaaa aaatttgtga taaaatgata
     3601 ttactcggcc gtttgccgct tattaaaatt ataaaaatca gtcttttcat cgaaggctta
     3661 aaacagggag atgtctttat ggatcaaatg cgtctgggaa aaacagagat tatggcttca
     3721 agatatggct ttggcgcgct tcctatccag agggtgagca gggaagaggc agcgctgctt
     3781 cttcgaaaag ctttcgatca cggcgtcaac ttttttgaca ccgcgagagc ttataccgac
     3841 agtgaagaaa aaatgggcta tgcgctctcg gatgtccgaa gccatattta catcgcgaca
     3901 aaaacccatg cgaaagacaa aaaaacatta tttgagcatc ttgaactgag cctgaaaaag
     3961 cttcaaaccg attatattga tgtctatcag ctgcataacc cggccgcgct ggattatgag
     4021 aatccggacg gcgtcttagc ggggcttgcg gaagcaaagc ggaaagggtt ggttcggttc
     4081 atcggcctga caagccaccg tctcagtctt gcgatgaaag cggctgcatc cggccggatt
     4141 gatacaattc agttcccgct gagcatgctc tcttcaagcg aagatatcgg tttaacggaa
     4201 gaatgcagga aatatgacgt cgggctgatt gccatgaaag ccatgtcggg cgggctgatc
     4261 cggcacccgg aagcggcgtt tgcgttttta aggcaatatg ccaatgtgct gccgatctgg
     4321 ggcgtccagc gagaagagga actggacgaa tttttgggtt atgaaaaaaa tcctccggcg
     4381 ctggatgacc ggatgaagca attgatcgaa gacgagcgcc gggaactttc cggtgatttc
     4441 tgcagagggt gtggttactg ccagccatgt gccgccggaa tcccgattga gaccgccgcg
     4501 cgtttgactc tgctgatgtc aagagcgccc tatgagcagt tcctgaccga tgactttcgg
     4561 gagcagatgg atcggatcga gaactgcacg ggctgcggtc actgccgctc tcattgtccc
     4621 tatggcctgg atacgcccgc tctcctgcgc cgcaatctgg aggattaccg ggcgttccgt
     4681 atgaagcatg gctgttagat gatttttaag agatatcttc ttaagttgaa agcatttcaa
     4741 agcgtcataa ggcttttctt ttacgagaaa tattagttta gtgatccaaa aagcgcccgc
     4801 tcaaacagag cgggcgcttt tgaatcctaa aaatgataag tgattatata tgcctggcac
     4861 atacataacg ggcggcccag tatccggaaa acagcggctg cacctgcacg cgataagcat
     4921 gaccggtatt cgctgcaatg aagtttccgc ctccgacata gataccgacg tggttgatac
     4981 cgcctccgct ggtcgcgaag aataccagat caccaggctg cagattgccc ttagacactg
     5041 cagttccata gccagactgt gctgcggcat catgcggaag atatttaccc gcgtatgcgt
     5101 aaacatattg tgtaaagccg gagcaatcaa aaccggaagg tgaaataccg cctgaaacat
     5161 atgggcaacc tacaaaaccc atcgcataac caacaatttt atttccgctg actgaggatc
     5221 ctccggtgga accgccgccg gaattcccgc tgccgctggt tccctgcagc tggcgcgcac
     5281 gggc
//
LOCUS       DATALY010000193         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_730380,
            whole genome shotgun sequence.
ACCESSION   DATALY010000193 DATALY010000000
VERSION     DATALY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730380"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1207)
                     /locus_tag="VHR42_05650"
     CDS             complement(71..1207)
                     /locus_tag="VHR42_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX3026694.1"
                     /translation="MILDEAREEIDKIDSQIMKLFSQRMDAVKIVGEIKKSQGLSVLQ
                     PERENQLLERIRENAREEYVYYSVALYKFLMDLSRTAQREIYLPDNSFTSTVLRRSNA
                     SPLKIEHPNIISQGIPGAFSGSAAAVLYPHGNFTFADSWEDVMVAIDEGSADYGILPL
                     ENSLAGSVTDVFDLLLKYQFYIVKATSIPVDHCLLGVRGANLSGISTVLSHPHALLQC
                     ENFFHDHPHIRKVPYMNTAMAANKVAKDGRLHQAAIASRECAHIYGLDILSKSIQMND
                     MNKTRFISVAKDPEFPDNMNKVSLAFSLPHVTGSLYRILARFALNGLNLTKIESRPNP
                     NKNFEYFFYLDFSGSVKSPATLRLLASLSEELSDFHFLGNYHEN"
     gene            complement(1243..2322)
                     /gene="aroC"
                     /locus_tag="VHR42_05655"
     CDS             complement(1243..2322)
                     /gene="aroC"
                     /locus_tag="VHR42_05655"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX3026695.1"
                     /translation="MSSIWGKNIRISIFGESHGAGIGVVIDGLPAGERIDMEEILFQM
                     SRRAPGRNNLSTPRKESDQPEIYSGFLENVTTGAPLCAIIKNNNTHSSDYQNLNQTPR
                     PGHADYTGRIRYNGFNDYRGGGHFSGRLTAPMVFAGAVCRQILFRRGIEIGAHIYSIA
                     EICDKPFDTAAVDSNVLNSIVKNKFPVLNHEAGERMCALIETARTQGDSVGGVIECAA
                     LGFPCGIGSPIFDGVENRLAGILFGIPAVKGIEFGAGFESSKLRGSENNDPFYFSGDN
                     VRTKTNRCGGILGGISTGMPLVFRLAVKPTPSIFQSQETVDLDTGRDTRLTIKGRHDP
                     CIVIRALPAVEALTAVCLLDMLEEC"
     gene            complement(2303..3574)
                     /gene="aroA"
                     /locus_tag="VHR42_05660"
     CDS             complement(2303..3574)
                     /gene="aroA"
                     /locus_tag="VHR42_05660"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3026696.1"
                     /translation="MANIILMPGGLDGRVTPPPSKSAAHRAIICASLSSGKSILKPFF
                     LCDDMSATIRAMRALGAEIQVQNRELSVDGSSTFERKDTTVDCGESGSTLRFTIPVAA
                     AGGGSFTFIGKGRLPSRPLGPYLDALPRAGVGLETHGGLPLRIKGRLQPGRFELPGNV
                     SSQFVTGLLLGLPLLDADSEIILSSPLESAGYVNLTIDVMRRFGVQVNTVETGYIVKG
                     NQLYTPCNFLVEGDWSQAAFWLAAGALGSRITCEGMNLNSLQGDRRIAGLLRQFGARL
                     TQDESEISISADRLQGIDIDASQIPDLVPVLAVVAALSRGITRIRNAGRLRMKESDRL
                     SATTKMLNTLGAKVTEQEDGLMIEGVEELSGGTVDGCNDHRIVMAAAVASVKCSNPVI
                     IIGSDCVNKSYPDFFEQYNRLGGNANVIHLG"
     gene            complement(3589..>4026)
                     /locus_tag="VHR42_05665"
     CDS             complement(3589..>4026)
                     /locus_tag="VHR42_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX3026697.1"
                     /translation="ILDEMICSNIKIKAGVVERDEREKGERMLLNFGHTLGHAIEKIY
                     QYETYSHGEAVAVGMVLMTRQAEKNGLTKPGNAEQIALLLKKYGLPDCCPKSLDDITG
                     AAINDKKRSGDKIRIVLLEEIGKSFVHTLPVSDIRRFFGLERD"
BASE COUNT          969 a         1016 c         1020 g         1021 t
ORIGIN      
        1 atctccaatt ttagtaaaac gcagcattca aacggtatgt tgaatgatag agcgatttga
       61 tttaaataat tcagttttcg tggtaatttc cgagaaagtg gaaatcgctc agttcctcac
      121 tgagtgaggc cagcagccga agcgtcgcgg gggatttcac gctgcctgaa aaatcaagat
      181 agaagaaata ctcgaagttt ttgttcggat tgggtcttga ttcaattttg gttaagttca
      241 gtccgttgag ggcaaaacgc gcaagaatcc gatagagtga gcccgtaaca tgcggcagcg
      301 agaaagcaag gctgacttta ttcatattat ccggaaattc cggatctttt gcgacggaaa
      361 taaaacgggt tttattcata tcgttcattt gaatggattt gcttagaata tccaagccgt
      421 agatatgggc gcattccctg gaggcgatgg ccgcttgatg gagtctgccg tctttggcga
      481 ctttatttgc cgccatggca gtgttcatat aaggaacctt gcggatgtga ggatgatcat
      541 gaaaaaaatt ctcacactgc agcaaggcgt gcgggtggga taaaaccgtg ctgatcccgc
      601 tgagatttgc gcctcttacc ccgagcaggc agtgatcaac cggaatcgac gttgccttga
      661 cgatgtaaaa ttgatacttc aacaataggt caaaaacatc cgttaccgag ccggccagcg
      721 agttttcaag cggaagaatt ccgtaatctg ccgaaccctc gtcaatggca accatgacgt
      781 cttcccagct gtcggcgaag gtaaagtttc catgcggata aagaacggct gcggccgagc
      841 cggaaaaagc ccccggaatg ccttgtgata tgatgttcgg gtgttcgatt ttcaacgggc
      901 ttgcgttaga ccgtcttaaa accgttgagg taaatgaatt gtcgggcaga tagatttcgc
      961 gctgggcggt tctgctcaga tccatcagaa atttataaag cgcaactgaa taataaacgt
     1021 attcctcccg cgcgttttcc cgaatgcgtt cgagcagctg attttcacgc tcaggctgta
     1081 acaccgatag cccctgggac tttttgattt cccccacgat ttttacagcg tccatccgct
     1141 gggaaaacaa tttcatgatt tgactgtcaa ttttgtcgat ttcttctctg gcttcatcta
     1201 gtatcattat tcggcccccg gtaatatgat tttggaatgg tttcagcatt cttccagcat
     1261 gtccagaagg cagaccgcag tcagcgcttc caccgcagga agcgcccgga taacaatgca
     1321 ggggtcgtgc cttcctttga tcgtcagacg ggtatcccgg ccagtatcaa gatccacagt
     1381 ttcctggctc tgaaaaatag agggcgtggg ctttacggca agacggaaaa caagcggcat
     1441 tccggtagaa atacccccga gaattcctcc gcaccgattg gtcttggttc ttacgttatc
     1501 tccgctaaaa taaaagggat cgttgttttc gctgccgcga agttttgatg attcgaatcc
     1561 ggcgccgaat tcgatcccct taacggctgg aattccgaac aaaataccgg ccagcctgtt
     1621 ttccactcca tcaaaaatgg ggctgccgat tccgcagggg aaacccaaag cagcgcattc
     1681 gataactccg ccgacggaat caccctgtgt tctggccgtt tcaatcagtg cacacattct
     1741 ttcaccggcc tcatggttga gaacggggaa cttatttttt acgatactat taaggacatt
     1801 actgtcgacc gccgcagtgt caaaaggttt atcgcagatc tcggcaatgg aataaatatg
     1861 agcgccgatt tcaatgccgc gtctgaaaag gatctggcgg cagacggccc cggcaaaaac
     1921 catcggtgcg gtgagtctgc cggaaaaatg gcctcctccg cggtaatcgt taaaaccgtt
     1981 gtaacggatt cttccggtgt aatcggcatg ccccggcctt ggagtctgat tcaggttttg
     2041 ataatcggaa gaatgtgtat tattattttt tattatagca caaagagggg cgccggtcgt
     2101 aacattttca agaaaaccgg aataaatttc cggctgatcg ctttctttac gcggcgtcga
     2161 aaggttgttg cggccgggag ctctgcggga catctgaaac agaatttctt ccatgtcaat
     2221 ccgttcaccc gccggcaggc cgtcgatgac cacgccgatt ccggcaccgt gggattcgcc
     2281 gaatattgaa atccttatat ttttacccca gatggatgac atttgcattc cctccaagtc
     2341 ggttatactg ctcaaagaaa tcgggatacg atttgtttac gcaatcgctg ccgatgatga
     2401 tgacagggtt gctgcatttg accgacgcga cagccgccgc catcacgatt cggtgatcgt
     2461 tgcagccatc gaccgttccg cccgagagct cttccactcc ttcaatcata agaccgtctt
     2521 cttgctccgt tacttttgcg cccagtgtgt tgagcatttt tgtggtagcg ctgagccggt
     2581 cgctttcttt cattcttaat cgtccggcgt tgcggattcg ggttatgcct ctgctgagag
     2641 cggcgaccac cgcgagcacc ggaacaagat cgggaatctg agaggcgtca atatcgatac
     2701 cctgaagccg gtctgccgag atagaaattt ccgattcgtc ctgcgtgagg cgggcaccga
     2761 attgacgcag aagcccggca atccgccgat cgccctgaag ggagtttaaa ttcatgcctt
     2821 cgcaggtaat gcgtgagccg agagctcccg ccgccagcca gaatgccgcc tgagaccagt
     2881 ccccttctac aaggaaatta caaggagtat aaagctggtt tccttttaca atataaccgg
     2941 tttccacagt gttaacctgt acgccgaatc gtctcattac atcaatcgtc agattgacat
     3001 aaccagcgga ttccagcggg gaggaaagga taatttcact gtccgcgtca agaagcggaa
     3061 gccccaggag cagcccggtg acgaactggg agcttacgtt gcccggaagt tcaaatctgc
     3121 ccggctgcaa tctacctttg atgcgaaggg gaagtcctcc gtgcgtttcc agaccgactc
     3181 ccgcccgggg gagagcgtcg agatacggcc ccagcggtct tgagggcagg cgtccttttc
     3241 cgataaaagt gaagcttccc ccgccggcgg cggcaacagg aattgtaaag cgaagggtgg
     3301 aaccggattc accgcagtcg acggtcgtgt ctttccgctc aaaagtagaa gagccgtcga
     3361 ccgatagctc tctgttttgc acttgaattt ccgccccaag agctcgcatg gcgcggatcg
     3421 tggcggacat gtcgtcgcat aagaagaaag gtttcaggat gcttttcccc gaggatagtg
     3481 aagcacagat gatggccctg tgagccgcac ttttggaagg cgggggcgtt acccggccat
     3541 caagcccgcc gggcatcaga atgatgttag ccacttagct ctcctccatt aatcgcgttc
     3601 taatccgaaa aacctccgga tgtctgagac agggagcgtg tgaacaaagc ttttaccgat
     3661 ttcttcaaga agcacaatcc ggatcttgtc tcccgatctc tttttatcgt taatagcggc
     3721 cccggtgata tcatccaaag actttgggca gcaatcgggc agaccgtatt ttttcagaag
     3781 caatgcgatt tgttcagcgt ttcccggttt cgtcaggccg tttttctcag cctggcgcgt
     3841 cataagcacc ataccaacgg cgacagcttc tccgtgggag taagtctcat actgatagat
     3901 tttttcgatg gcgtggccaa gcgtatgccc aaagttcagg agcatccgtt cacctttttc
     3961 ccgttcatca cgctctacaa cgccggcttt gatctttata ttagagcata tcatttcatc
     4021 aaggat
//
LOCUS       DATALY010000194         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_128329,
            whole genome shotgun sequence.
ACCESSION   DATALY010000194 DATALY010000000
VERSION     DATALY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128329"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..2488
                     /gene="pheT"
                     /locus_tag="VHR42_05670"
     CDS             110..2488
                     /gene="pheT"
                     /locus_tag="VHR42_05670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX3026698.1"
                     /translation="MKLSMKWLSDYVDINVAPKDFSEAMTMTGSKVELFENTGDKIDK
                     VVAGTILSVESHPNADKLVICKLDVGSGSPIQIVTGARNVFAGANVPVALDGSSLPGG
                     VKIRKGKLRGEESNGMMCSIGELGLTLNDFPYAEENGIFIMKEECRPGQDIREVLGMD
                     DTVVEFEITPNRPDCLSVLGLAREVAVTYDKAMTLPDTAVEGKEGKTTDFVTVEVEEP
                     SLCTRYTARAVRNVRIGPSPRWMRERLRAAGVRPINNIVDITNYVMLEYGQPMHAFDY
                     RFIKDGRIHVRRAHAGEKIVTLDGVERTLADSMLVIADENRPVAVAGVMGGEHSGIMD
                     DTNTIVFESAMFDGTSVRLTSKKLGLRSESSARFEKGLDAQNTIPALMRACRLVELLG
                     AGEVCGDMIDVDNSDHSVRTVPLDADWINRFLGTDIPEDFMINALKKLEFHVENRQIT
                     VPTYRSDVENKADIAEEVARIYGYNKIPVTMIGGSTPKGGLNPAQKFEKNMTEALISL
                     GFYEVSTYSFISPKYYDKILLPADSPMRKSIRILNPLGEDTSIMRTTTLPSILEVMSR
                     NFSNRNTSAKFFEIGTVYLPQEGQKLPQEATRITMGMFGQGTDFYVLKGAVEELLEQA
                     GVGEYEIEADSDDKAFHPGRCARISIDGKSLAVFGEIHPAVQSNYEMDVRCYAAVIDF
                     ELLMECSSHEKNYKPLPKFPAVVRDIALICDDNIPVLELEKAIRNAAKNVLERIELFD
                     IYKGKQIPDGKKSVAFSVTMRAADRTLTDEEADSVMKKIFRLLEKMGAALRA"
     gene            2547..2792
                     /locus_tag="VHR42_05675"
     CDS             2547..2792
                     /locus_tag="VHR42_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEX3026699.1"
                     /translation="MHEPTMVFSIKEDKEKEMKKILVTVYDALREKGYNPVNQIVGYL
                     LSEDPTYITTHNNARSLIRKIDRDELLHVLVESYLND"
     gene            3008..>3312
                     /locus_tag="VHR42_05680"
     CDS             3008..>3312
                     /locus_tag="VHR42_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026700.1"
                     /translation="MTDKKTNKKDAFLTYKDKPLVRSGNILYYGNMSDKYVILMQILS
                     TKTVKGQEIAGNVSVQLTATDDEIRARDRIIKKTEKDGLYNAIDIGCIWLERALNEL"
BASE COUNT          919 a          713 c          879 g          801 t
ORIGIN      
        1 cttttctatg aaaatgatct gcgattcctc agccagttct gatttttatc cgaattaaaa
       61 tggcctttga ttaatgggcc gcctggcggc ggcggaaagg gttttagtca tgaaactttc
      121 aatgaaatgg ctttcagatt acgttgatat aaatgttgcg cccaaagatt tcagcgaggc
      181 aatgaccatg accggctcga aggtcgagct gtttgaaaat accggagaca agatcgacaa
      241 ggtagtggcc ggaaccattc tttcggttga gagccatccc aatgccgaca aattggtaat
      301 ttgcaaactt gacgtcggga gcggcagccc tattcagatt gtcaccggag cccgaaatgt
      361 gtttgccggg gcaaatgttc cggtcgcgct tgacggatca tcgcttcccg gcggagtcaa
      421 aatccgtaaa ggcaagctgc gcggagaaga gtccaacggg atgatgtgct cgattggaga
      481 actgggcctg actttgaatg attttcccta tgccgaggag aacggcattt ttatcatgaa
      541 ggaagaatgc cgccccggac aggatatccg cgaggtgctc ggaatggacg atacggtggt
      601 ggaatttgaa ataaccccca accggcccga ctgcctttcc gtactcgggc ttgcgcgtga
      661 ggtggcggtg acctacgata aggccatgac cctgccggat accgcagtcg agggcaaaga
      721 aggaaaaaca accgattttg tgaccgtaga ggtggaggag ccatcccttt gtacccgtta
      781 caccgcgaga gctgtgagaa atgtcaggat cggcccgtcg ccgcgctgga tgcgagagcg
      841 gctcagggct gcgggtgtcc gcccgatcaa caatattgtt gatattacga attatgtcat
      901 gctcgaatac ggccagccga tgcatgcatt tgactatcgc tttattaaag acggccggat
      961 tcatgtgcgc cgggcgcacg cgggagaaaa gattgtaaca ctcgacgggg tggaaagaac
     1021 ccttgccgac agcatgctgg tgatcgccga tgaaaacagg ccggttgccg tcgccggggt
     1081 aatgggcggc gagcatagcg gaattatgga cgataccaac accatcgtgt ttgaatcggc
     1141 catgtttgac ggaaccagcg tccgcttaac atcgaaaaaa ctggggctga gaagtgaatc
     1201 gtccgcccgc tttgaaaaag ggcttgacgc ccaaaatacc attccggcgc tgatgagggc
     1261 ctgccgtctg gtggaactgc tcggcgcggg agaagtatgc ggagatatga ttgatgtcga
     1321 caattccgac cattcggtca ggactgtgcc gctcgacgcc gattggatca atcgctttct
     1381 gggaacagat atccccgaag acttcatgat caatgccctg aaaaagctcg agtttcatgt
     1441 ggagaaccgg cagattactg tgccgactta tcgttcggat gttgagaata aggccgatat
     1501 tgcggaagag gtcgccagaa tctacggtta taataaaatc ccggtgacga tgatcggggg
     1561 ctcgactccg aaaggcgggc tgaatcccgc gcagaaattt gaaaaaaata tgaccgaagc
     1621 actcatttcc ctcggattct acgaagtttc gacctattcg ttcatcagcc cgaaatatta
     1681 tgataaaatc cttttgccgg ctgattcgcc gatgcggaaa tcgatccgta ttctcaatcc
     1741 gctcggtgag gatacttcga ttatgcgcac cacgacgctg ccgtcgattc tggaagtcat
     1801 gtcccggaat ttcagtaacc gcaacacctc ggcaaagttt tttgagatcg gcactgttta
     1861 tctgccgcag gaagggcaga aactgcctca ggaagccacc cggatcacta tggggatgtt
     1921 tggtcagggt acggatttct atgtgcttaa gggagcggtc gaagagcttt tggaacaggc
     1981 cggtgtcgga gaatatgaga ttgaggcaga ctcggacgac aaagcgttcc atccgggccg
     2041 atgcgcgaga atttccattg acgggaaaag ccttgcggtt ttcggtgaaa ttcatcccgc
     2101 cgtgcagtcc aattatgaga tggatgtccg gtgttatgcg gcggtgattg actttgagct
     2161 tctgatggaa tgctcgagtc atgagaaaaa ttataaacct ttgccgaaat ttccggcggt
     2221 cgtaagggat attgcactca tttgcgacga taatattcct gttcttgaat tggaaaaagc
     2281 aattcggaat gcggcaaaaa atgttctcga acgtattgaa ctgtttgata tttataaggg
     2341 aaaacaaatt ccggacggca aaaaaagcgt tgctttcagc gtgacgatgc gcgccgcgga
     2401 ccggacgctc accgatgagg aagcggattc tgtgatgaag aaaatcttcc gtttgctgga
     2461 aaaaatgggc gctgcgctgc gtgcttaaat tttgcttgca aaaccgcaaa aaagagagta
     2521 tgctagtatg ctaagggggt gtaattatgc atgagcctac catggttttt tcgattaaag
     2581 aggacaagga aaaggaaatg aaaaagattc ttgtcacggt atatgatgcg ctgagggaaa
     2641 aaggctataa cccggtgaat caaatcgtgg gctatcttct ctcggaggat ccgacctata
     2701 tcactacgca taataacgcc cggagcctaa ttcgcaaaat tgaccgtgat gaattactgc
     2761 atgtacttgt agaatcctat ttaaacgatt gattagacga ctggcaagag cggccttgat
     2821 tatcaaggcc gctcttgctt tttaaaaccc aatggaatgg atattttcgt attttacaaa
     2881 attatacaaa acaatcggat taaggtttac atagcatctt aattgtgata aaattaaata
     2941 tacgcctata gccggtaaaa cagtgttttt gatatttgac caccataatt tgattggagg
     3001 ataaagaatg acggataaaa aaacaaataa gaaagacgca tttttaacat ataaggacaa
     3061 accactggtt cggagcggca atattcttta ttacggaaat atgtccgata aatatgttat
     3121 cctgatgcag attttaagca ccaaaacggt aaagggccag gaaatagccg gaaatgtttc
     3181 cgttcagctt accgcaaccg acgatgagat cagagcgagg gacagaatca ttaaaaaaac
     3241 ggaaaaagac ggtctttaca atgcgattga cattggatgt atctggcttg aacgcgctct
     3301 caacgaacta ta
//
LOCUS       DATALY010000195         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_647038,
            whole genome shotgun sequence.
ACCESSION   DATALY010000195 DATALY010000000
VERSION     DATALY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647038"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VHR42_05685"
     CDS             <1..1877
                     /locus_tag="VHR42_05685"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HEX3026701.1"
                     /translation="KNVCTLGPIIHNPQLVADLEKKGAWAVASPADVPDGSTVVIRTH
                     GVAQEVYTGLENRRLNIIDATCPFVSKIHSIVRDASSEGSIVLIAGNPSHPEVEGIRG
                     HSSSDVYVFGSRKELEEITAAHPEIVSQKIIAVSQTTFNTREWELCRNFIEKVYTNAK
                     IFDTICNATSIRQNEAKALAQESDMMIIIGGRQSSNTAKLYAVCSEICPSYLVEGASE
                     LPFDKLKNCLRVGVTAGASTPAWIIKEVLKAMTDMEKDNGDISFAEALEKSFKTLSND
                     EIVQGVVIGISQSEIEVDLGTKHAGYVPLNEMTDDSNVKAEDLVKVGDELDLVVMRVN
                     DQDGTVMLSKKRFDAIRGWQDIVKAEEDGTVLDGTVVDVVKGGLLVLCSGIKIFVPAS
                     HSGVSKNDNLEELLKKPVRMKIIEISKGRRRAVGSIRAVQREEKKELAEKFWNEAEIG
                     KTYTGKVKSLTSYGAFVDLGGVDGMIHVSELSWSRINHPSEVLNIGDNVEVVIKDIDR
                     ESKKISLSYPNKGENPWNKFAADYKAGDIVEVKIVSFMPFGSFAQIFPGVDGLIHISQ
                     IADRHIAKASDVLEIGQKVKVKILEINEEKRRISLSIKEAADANGEDFPANEEPEENS
                     "
BASE COUNT          556 a          425 c          546 g          464 t
ORIGIN      
        1 aaaaaaatgt ctgtacgctt gggccgatta ttcacaatcc gcagctggtt gctgatttgg
       61 agaaaaaagg cgcgtgggcg gttgcgtctc ccgccgatgt tccggacggc tcgacggtgg
      121 tgatccggac acacggagtg gcgcaggagg tttacaccgg gctcgaaaac cgcaggctga
      181 acatcattga cgcgacctgc ccttttgtgt cgaaaatcca ttcgatcgtc cgcgacgcct
      241 ccagtgaggg cagcatcgtg ctgattgccg gaaatccgtc ccatcccgaa gtagagggaa
      301 tccgggggca ctccagttct gatgtttatg tgtttggcag ccggaaagag ctggaggaaa
      361 taacggcagc tcatccggag atcgtgtcac aaaagattat tgcggtgtct caaaccacat
      421 tcaataccag agaatgggaa ctgtgccgta attttatcga aaaagtatat acaaacgcaa
      481 agatatttga tacaatatgt aatgcgacat caataaggca aaatgaggcc aaagcgctcg
      541 ctcaagagtc ggatatgatg atcataatcg gcggcaggca aagctccaac acagccaaac
      601 tttacgccgt ttgttccgaa atttgtccgt cctatctggt cgagggcgca tcggagcttc
      661 cctttgacaa actcaaaaac tgtttgcgtg ttggtgtcac ggcgggggcg tccactcctg
      721 cctggataat aaaggaggtt cttaaagcga tgaccgatat ggaaaaagac aacggagata
      781 tcagctttgc cgaggcgctt gaaaagtcat ttaaaacact gagcaatgac gaaatcgttc
      841 agggagtggt tatcggcatt tctcagtctg agatagaagt tgatcttgga acaaaacatg
      901 ccggctatgt gccgttgaat gagatgaccg acgattccaa tgtgaaagcc gaagacctgg
      961 tcaaagtggg tgatgagctt gacctggttg tcatgcgtgt gaacgatcag gatggcaccg
     1021 ttatgctctc caaaaagcgt tttgacgcga ttagaggctg gcaggatatt gtcaaggccg
     1081 aggaagacgg gacggttctt gacggaacgg ttgtcgacgt ggtcaagggg ggccttttgg
     1141 ttctctgcag cggaattaaa atatttgtgc ccgcgtctca ctcgggcgtt tcgaaaaacg
     1201 acaatctcga agagcttctg aaaaagcctg ttagaatgaa gatcatagaa atcagcaagg
     1261 gccgcaggcg tgcggtcggt tcgatccgcg ctgtccagag ggaagagaag aaggaattgg
     1321 ccgagaagtt ctggaacgaa gcggaaatcg gcaagaccta taccggaaag gtaaaatccc
     1381 ttacatctta tggtgcgttt gttgatcttg gcggagttga cgggatgatt catgtatccg
     1441 aactttcctg gtcgagaatc aaccatccct ccgaagtgct gaacatcggc gataatgttg
     1501 aagttgtcat taaagacatc gaccgcgagt cgaaaaaaat atcactaagc tatccgaata
     1561 aaggcgagaa cccgtggaat aagtttgcag ccgattacaa agcgggcgat attgttgagg
     1621 ttaaaattgt cagctttatg ccgttcggtt cctttgctca gatttttccc ggagttgacg
     1681 gcctgatcca tatatcccag attgccgacc ggcatattgc caaggcgtcg gatgttttgg
     1741 agattgggca gaaggttaaa gtgaaaatcc ttgagatcaa cgaagagaag agaaggatca
     1801 gcctttcgat caaagaagcg gctgacgcca acggcgagga tttcccagcc aacgaagaac
     1861 cggaagaaaa ttcataacgt aaaatgtaaa aaaacgacgg agattttctc cgccgttttt
     1921 ttgtgctttt tgtgtgtctg aaagcaacag aacaatttgc tcatgaatac gaaggaaagg
     1981 tcaggccgcc g
//
LOCUS       DATALY010000196         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_474549,
            whole genome shotgun sequence.
ACCESSION   DATALY010000196 DATALY010000000
VERSION     DATALY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474549"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VHR42_05690"
     CDS             <1..1677
                     /locus_tag="VHR42_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX3026702.1"
                     /translation="TMEVYAPLAHRLGIRTIKEELEDISLRHLDSVAYHEIEAALETR
                     KEEREALLEGIKDRISDRLKGLNLNVFLEGRVKSVYGIYRKIYLQGKAFDEIYDIYAV
                     RVIVDTVNECYNVLGIIHDMMRPIPGRFKDYISTPKPNMYQSLHTTVIGKEGIPFEVQ
                     IRTWEMHHTAEYGIAAHWKYKLGISGKDTLDQRLEWVRQLLEIQTEARDAEDFIRSIK
                     TDLAPDEVFVFTPKGDVINLPSRSTTIDFAYAIHSAVGHRMIGAKVDGKIVPINYELK
                     TGEIIEVLTTSSPNHGPSRDWLKIVRTSEARNKIRAWFKKERREENITEGREELEKEF
                     KRNGILIYDGQYDEFVLSIAKRQHFNNIEEFLAAIGYGGVMLSKIMPRIKDDYIRLVK
                     PQSIQLPENVTPRHTTGGIIVEGLENCLVKIARCCNPLPGDRIVGFVTRGFGVSVHKK
                     DCPNVVQNMNNEDYSGRWVKVHWADNVQSSFKASLQINSVNRCGLLADITAALSSMRV
                     MIHGINARELKNGDLFINLTVDVTSIAHLQSVITKIGKISGVLDVSRSRG"
     gene            1723..3003
                     /locus_tag="VHR42_05695"
     CDS             1723..3003
                     /locus_tag="VHR42_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX3026703.1"
                     /translation="MNCSFQNLGFYPADILLPEGVDMTKWSVIACDQYTSEPEYWDKV
                     ESTVGGNPSTLRLTMPEIYLGSGNVDERIEKINETMKDYRRRGLFRKVEDSMIYVERR
                     LANGKIRKGLVGVVDLEDYDYNAGSSTLIRATEGTVLDRIPPRVRVREGAELEVPHVM
                     LLVDDPDHTVIEPVSDARDGLEKLYDFHLMQGGGSITGYRVEGERLSGIAGALKALAR
                     PEVFEAKYGAPGKPVLVYAAGDGNHSLATAKECYSRIKATLPPEQAKNHPARYALVEL
                     VNLHDSSLEFEPIHRVVFDVDPEKLLKAFFEAFPNARKGEGPGHRVEYLCASASGVIT
                     CEYPKSNLAVGMLQAFLDGYLKNNGGRVDYIHGEAAVKALSSEPGRIGFLLPPMEKSD
                     LFKTVVFDGVLPRKTFSMGEADDKRYYLECREIR"
     gene            3043..3492
                     /gene="dtd"
                     /locus_tag="VHR42_05700"
     CDS             3043..3492
                     /gene="dtd"
                     /locus_tag="VHR42_05700"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX3026704.1"
                     /translation="MKAVIQKVTRASVTVDDRVTGEIGDGFLILLGIKKGDATVEADL
                     LADKIACLRIFPDEKGKLNRSLQDVGAGALVISNFTLYADCSSGRRPDFQKAAPYAEA
                     NVLYEYFCDRLRRDGIPCQTGEFGADMKVDLLNDGPITIVIETDELK"
     gene            3520..4164
                     /locus_tag="VHR42_05705"
     CDS             3520..4164
                     /locus_tag="VHR42_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3026705.1"
                     /translation="MLVKNLSVGPIETNCYILCDETAKKAAVIDPGFEAEKILEELGR
                     TGCSAEYVILTHGHFDHISAAGQVAGETGAKLAAFAEEEDFIKDPAANLSSGFGKGIF
                     KPLEPDILVRDGETLALGSMTLRFLLTPGHTGGSGCILCGDCLFTGDTLFRGGAGRTD
                     FPTGDYPSLIKSLRRLASLKGDYRVYPGHGGITSLNAERRSNPYLAEGSDDTVF"
     gene            4148..>4507
                     /locus_tag="VHR42_05710"
     CDS             4148..>4507
                     /locus_tag="VHR42_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026706.1"
                     /translation="MTLFFEGHDFRYEASNLCYLFFPGEKVEIAAPEEEKSGDWAYIA
                     IRRRRRRIYATVIISRGEKNERALSSFDCTGFSEGAVDPKECVYAMGRAFYRAASRLC
                     GFYPPWGILTGIRPVKLA"
BASE COUNT         1119 a         1107 c         1284 g          997 t
ORIGIN      
        1 accatggagg tttatgcgcc gcttgcccat cgcctgggca tccggaccat caaggaggaa
       61 cttgaggata tctcgctgcg gcatctggac tcggtagctt atcatgagat cgaggccgcc
      121 cttgaaactc ggaaggagga gcgggaggcc cttctggaag gtatcaaaga ccgcatttcc
      181 gaccggctta agggtttgaa tctgaatgtc ttccttgagg gaagggtcaa gagcgtttat
      241 ggaatttatc gaaaaattta tcttcagggg aaggcatttg acgagattta cgatatctat
      301 gccgtgaggg tcattgttga tacggtcaac gaatgttaca acgtgctggg tatcatccac
      361 gatatgatga ggccgattcc gggccggttc aaggattata tttcgacccc caagccaaac
      421 atgtatcagt cgctccacac aacggttatt ggcaaggagg gaatcccttt cgaggttcag
      481 atccgaacct gggagatgca tcacacagcg gaatacggca tcgccgctca ctggaaatat
      541 aaactcggaa tctccggtaa agacacgctc gaccagcggc tcgaatgggt gcgccagctg
      601 cttgagatcc agaccgaggc cagagatgcc gaggatttta tccgttcaat caaaaccgat
      661 ctcgcgccgg atgaggtgtt tgtgtttacc ccgaaagggg atgtcatcaa cctgccgtcc
      721 cgttcgacca ccatcgactt cgcctacgcg attcacagcg cggtcggtca ccggatgatc
      781 ggcgccaagg tcgacggaaa aatcgttccc atcaactatg aactcaaaac cggagagatc
      841 atcgaagttc tgacaacctc ttccccgaat cacggcccga gcagggactg gctcaaaata
      901 gtcagaacca gcgaagcccg gaataaaatc cgggcgtggt tcaagaaaga gcgcagggag
      961 gaaaatatca ccgagggccg cgaggaactg gaaaaagaat tcaaacggaa tggaatcctg
     1021 atctacgacg ggcagtatga tgaatttgtc ctgagtattg ccaaacggca gcatttcaac
     1081 aatattgaag aatttcttgc cgcgatcggt tacggcgggg ttatgctttc caagattatg
     1141 ccgagaatta aagatgatta tatcaggctg gtaaagcccc agtcgattca gcttccggaa
     1201 aacgtaacgc cccggcacac gaccggcgga attatcgttg aggggcttga aaactgtctg
     1261 gtcaagatcg ccaggtgctg caaccccctt ccgggcgacc gtattgtcgg gtttgtgacg
     1321 cgtggattcg gcgtttccgt ccataagaag gattgcccca atgtggttca gaatatgaac
     1381 aatgaagatt attccggccg ctgggtcaag gttcattggg ccgataatgt tcaaagctca
     1441 tttaaagcgt cacttcagat taattctgtc aaccgctgcg gcctgctggc cgacataacg
     1501 gcggcgttat cgtccatgag ggtcatgatc cacggaatca atgcccgtga gctcaaaaac
     1561 ggcgaccttt tcattaacct tacggtcgat gtaaccagca tcgcccatct gcagagcgta
     1621 atcacgaaaa tcggaaaaat cagcggcgtt ttagatgttt cccgatcaag aggctgaccg
     1681 taaaacgtcc tgtttacttt acatcaaaac ggagtgaatc aaatgaattg ttcgtttcaa
     1741 aatcttggct tttacccagc ggatattcta ctgccggaag gcgtggatat gacaaaatgg
     1801 agcgtgatcg cctgcgatca gtatacctcc gagccagaat actgggataa agttgaaagt
     1861 acggtgggcg ggaacccatc caccctgcgc ctgacgatgc cggaaattta tctgggcagc
     1921 ggaaatgtgg atgagagaat tgaaaaaatt aatgaaacga tgaaggatta tcgccgccgc
     1981 ggccttttcc gtaaggttga ggattccatg atctatgttg aacgcagact ggcaaacgga
     2041 aaaatcagaa aaggcctggt cggcgtggtc gatctcgagg attatgatta taacgccggt
     2101 tcgagcacgc tgatccgcgc aacggaggga accgttcttg accggattcc gccccgggtc
     2161 agggtgcgtg agggcgctga gcttgaggtt ccccacgtca tgctgctggt ggacgaccct
     2221 gatcataccg tcatcgagcc ggtgtccgac gcgagagacg ggcttgagaa gctttatgat
     2281 tttcatctga tgcagggcgg cggaagcatt accggctacc gtgtcgaggg agaacggctg
     2341 tccgggatcg ccggagcgct gaaagctctg gcccgccccg aagtgtttga agcaaaatac
     2401 ggtgctccgg gaaaaccggt tctggtctac gccgccggag acggaaacca ttcccttgcg
     2461 actgcgaaag aatgctatag cagaatcaaa gcaaccctgc cgccggagca ggccaaaaac
     2521 catcccgcgc gctatgctct ggtcgagctt gtcaatctgc acgattcctc cctggagttc
     2581 gaaccgatcc atcgtgtggt gttcgacgtt gacccggaaa aacttctaaa ggcgtttttt
     2641 gaagcgtttc ccaacgcacg caaaggcgaa ggccccggtc accgggtgga atatctctgc
     2701 gcctcggctt ccggcgttat cacctgcgaa tatccaaaga gcaacctggc tgtcggaatg
     2761 cttcaggcat tcttagacgg atatctgaaa aacaatggcg gacgtgtgga ctatatccac
     2821 ggggaagcgg cggtgaaagc gctgtcgtcc gagccgggcc gaatcggatt ccttctgccc
     2881 ccgatggaaa aatcggatct gtttaaaacc gtggtgtttg acggtgtgct gccgcgaaaa
     2941 acgttctcca tgggcgaagc ggatgacaaa agatattatc tggaatgccg cgaaattcgc
     3001 tgaatggttg cgggtttcca ggaatgaaaa acggaggcgg ctatgaaagc agtcattcag
     3061 aaagtaacaa gagcaagcgt tacggttgac gatagggtca cgggtgaaat tggtgacgga
     3121 ttcctgatcc tgctcgggat caaaaaaggg gacgccacgg tcgaagcaga cctgctcgcc
     3181 gataaaatcg cctgcctgag aatctttccc gacgagaagg gaaagctgaa ccgttcgctt
     3241 caggatgttg gcgcgggtgc gctggtgatt tcaaacttta ccctttatgc cgattgctcc
     3301 agcggaaggc ggccggattt tcaaaaagcc gccccttatg ccgaggcaaa cgtgctctat
     3361 gaatatttct gtgatcggct cagacgggat ggaattccct gccaaacggg tgaattcgga
     3421 gcggatatga aggtcgatct gctcaacgac gggccaatta ccattgtgat tgaaacggac
     3481 gagcttaaat aagcgcgccc gggaacggga gggaacagca tgttggtcaa aaacctcagc
     3541 gtagggccca tcgaaaccaa ttgttatatt ctctgcgatg aaacggcgaa aaaagcggca
     3601 gttattgatc cgggttttga agctgaaaag atacttgagg aactcgggcg gacaggctgc
     3661 tccgccgagt atgtaattct gacccatggc cattttgatc atatttcagc cgccgggcag
     3721 gtagccgggg aaaccggggc aaagcttgcc gcgtttgcgg aagaggaaga ctttataaaa
     3781 gatcccgcgg ccaatttgag ctccggtttc ggcaagggaa tcttcaagcc cctcgaaccg
     3841 gacattttgg ttcgggacgg tgaaacgctc gcgctgggat ccatgaccct gcgttttctc
     3901 ctgaccccgg ggcatacggg aggctcgggc tgtattcttt gtggagactg cctgttcacc
     3961 ggggacacac tgttccgggg cggcgccggc cgtaccgatt ttcccaccgg ggattatccg
     4021 tcgctgataa aatcacttcg ccgtcttgct tctctgaaag gggactatcg cgtttatccg
     4081 ggtcacggag ggattacaag ccttaacgct gagaggcggt ccaatcctta tttggcggag
     4141 ggctcagatg acactgtttt ttgaaggcca tgacttccgg tatgaagcct ccaacctctg
     4201 ttacctgttc tttcccggag aaaaagtgga aattgcggcg ccggaagaag aaaaatcggg
     4261 cgactgggcg tacattgcga tccggagacg ccgtcgaagg atttacgcga ccgtaatcat
     4321 cagccgcggg gagaaaaacg agagagcgct gagctcgttt gactgcacgg ggttttcgga
     4381 aggagcggtt gatccgaaag agtgcgttta cgctatggga cgtgcatttt acagggcggc
     4441 gtcacggctt tgcggtttct atccgccctg gggcatcctg acgggtatcc gaccggtcaa
     4501 gcttgcc
//
LOCUS       DATALY010000197         7267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_559876,
            whole genome shotgun sequence.
ACCESSION   DATALY010000197 DATALY010000000
VERSION     DATALY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559876"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VHR42_05715"
     CDS             <1..734
                     /locus_tag="VHR42_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX3026707.1"
                     /translation="SLGKLQGKMPWLRGRVVRRRGVPGLQKLRIRFRKTGRAKYISHL
                     DLNRFMSRALRRAEIPVWMTQGFNPHPYITFALPLSLFYESLCEVMDSKLDGEMSFEE
                     IAERLNRQMPEGIEIYEVCEPVLKVTELAYASYQIRMEFPGLEKQELEEAVGRLQNSD
                     KILVTKTTKRHEIEIDLKEIFTQAKISAGTGSLLIEATVPASPQENLNPSCLLAAIEK
                     YAGLTPEIEYITRTGIYNNGMEIFR"
     gene            957..1301
                     /gene="rplS"
                     /locus_tag="VHR42_05720"
     CDS             957..1301
                     /gene="rplS"
                     /locus_tag="VHR42_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX3026708.1"
                     /translation="MDAMKHFVNAQMKESIPEFHIGDTVKVHVKIKEGERERIQVFEG
                     TVIARKGGGISESITVRRVSYGVGVERVFPLHSPKVDKIETIRHGRVRRSKLYYLRDR
                     VGKAAKVKEQIR"
     gene            1463..2038
                     /gene="lepB"
                     /locus_tag="VHR42_05725"
     CDS             1463..2038
                     /gene="lepB"
                     /locus_tag="VHR42_05725"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3026709.1"
                     /translation="MSDINIQPDGSAAPQGYSLKNDVFEWMEAIVFSLSFVVLLFTFV
                     FRIVGVDGTSMLNTLQNGDRVVISDINYTPKRGDIIVLSTKAVEKPIIKRVIATEGQR
                     IDIDYVTHTVTVDGKALSEPYIAEPTVFQGQNPVSLPATVPKGHVFVMGDNRNYSLDS
                     RSSSIGMIDDRYILGRAVFRIFPFSGFGFLK"
     gene            2070..2966
                     /gene="ylqF"
                     /locus_tag="VHR42_05730"
     CDS             2070..2966
                     /gene="ylqF"
                     /locus_tag="VHR42_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEX3026710.1"
                     /translation="MTELPSIQWFPGHMAKTRRLIKESLHLVDIVAELTDARVPESGR
                     NPELDALCVSKPRILLLNKSDMADENITGEWLTYYRSKGYYALSVDCKNGRGLNRFMP
                     LIREILQEKLEKLADKGMSGRTLRVMVAGIPNVGKSTFINTMSHSKRVKVEDRPGVTR
                     GRQWISLGSGVEMMDTPGILWPKFEDPKVGEKLAFTGAVKDDVIDRELLCARLLEVLA
                     QSYSDELRARYKLSGPLPEDGTDLLSMIGRKRGMLISGGEVDGERASAMVLDEFRSGK
                     LGRITLEKPSDHTENAETGYEA"
     gene            2969..3592
                     /locus_tag="VHR42_05735"
     CDS             2969..3592
                     /locus_tag="VHR42_05735"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX3026711.1"
                     /translation="MEWFYYEHMARRESAGLICGIDEAGRGPLAGPVFAAAVILPDDL
                     EIAGINDSKKLSAKKREALFEVITKKAIDYFVAQASEAEIDTYNILNATYLAMKRAVE
                     GLKTKPDFALVDGNRDPVLGIPTTCIVGGDGKSASIAAASILAKVSRDRYMEELDRKC
                     PQYLFAKHKGYPTKLHYEKILQYGVSEYHRMSFLKNLAEKRLSFEQK"
     gene            3579..3944
                     /locus_tag="VHR42_05740"
     CDS             3579..3944
                     /locus_tag="VHR42_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009442486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX3026712.1"
                     /translation="MNKNDTGRLGELAAARYLRKAGCEILSANYRTRFGEIDLIASDG
                     RFLIFAEVKTRRENSLFLPREAVDQKKQEKIIAAAKLYLSVNQTKLQPRFDVIEVVTA
                     AGGEFQVLSIHQIENAFTL"
     gene            3980..4945
                     /locus_tag="VHR42_05745"
     CDS             3980..4945
                     /locus_tag="VHR42_05745"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX3026713.1"
                     /translation="MRYFDLHCDTITKCSDKNKELFHNDLHISLERAAGYATWAQFFA
                     VWTPDKTNGDEAEKRFWKTYAAFTTQIGKNKNLIDFCGNAKDLEQAVAAGKQIAMLSI
                     EGAVCLNGRLELIDEFYHKGVRLLSLTWNDRCITADGCMTPDAQGLTPFGREVIRRMG
                     RLGMVADVSHLSEKGFWEIARLTDRPFVASHSNSAAVCAHKRNLTDDQLKEIVNREGL
                     VGLNLYTEFIRRGKPPVRVEDLLSHIDHILSLGGENVLAIGSDFDGATMPQGIKGVED
                     MAKLHGMISEKFGADTADKIFFENAFRFFKTALTDCESCNNIIEL"
     gene            5138..6634
                     /gene="thrC"
                     /locus_tag="VHR42_05750"
     CDS             5138..6634
                     /gene="thrC"
                     /locus_tag="VHR42_05750"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX3026714.1"
                     /translation="MNYKSTRNSGISVTASSAILEGISADGGLYVPESTPWLSYNDIK
                     ALTDSDYVSRAADILGRFLNDFTNSEVMDCVSRAYTREKFETDSIAPIYMLENGVYLL
                     ELWHGPTSAFKDIALQLLPYLMKVSVSKAKTEKTVVILVATSGDTGKAALEGFKDVDG
                     TKIMVFYPENGVSPMQKRQMITQEGGNVAICAIKGNFDDAQSAVKEIFADADIRDKLS
                     ENNMMFSSANSINWGRLVPQIVYYVSSYCDLIKNNKITCGEQVNFVVPTGNFGNILAA
                     YYAKKMGIPVNKLICASNANNVLADFIQTGVYDRNRPFYTTISPSMDILISSNLERLL
                     FDLSGCDDAKIREWMSQLAETGRYEVDDTVKNSLHEHFWSGFCSDDDTKAVIKEIYAK
                     YAYVCDPHTAVALHVYEQYKKKTSDTTKTLIASTASPFKFAPNVLEAINGESGRLGER
                     EMLEKVAELAMCHIPKQIAGLFSKPERFRDETCKKTEMKEEIIRSLGL"
     gene            complement(6676..6864)
                     /locus_tag="VHR42_05755"
     CDS             complement(6676..6864)
                     /locus_tag="VHR42_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HEX3026715.1"
                     /translation="MNFINQIADKIGKNEHVRGIHCEVTNCVYHDRKGKCAADEIKVG
                     PTYASNSTETVCSTFEPK"
     gene            7005..>7267
                     /locus_tag="VHR42_05760"
     CDS             7005..>7267
                     /locus_tag="VHR42_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3026716.1"
                     /translation="MSLYAIGDLHLSFGSDKPMDIFTGWQDYVSKIKKNWENIVKETD
                     TVVIPGDVSWAMDFDSLRPDFDFIDRLPGSKLIIKGNHDYWWST"
BASE COUNT         1912 a         1646 c         1982 g         1727 t
ORIGIN      
        1 gaagtctcgg aaaactgcag ggaaaaatgc cttggctgcg gggccgcgtc gttcgccgaa
       61 ggggggtgcc cggtttgcag aaattaagaa tccgattccg aaagaccggc cgggcgaaat
      121 atatttccca cctcgacctg aaccggttta tgtcccgggc gctccggcgt gccgaaattc
      181 ccgtctggat gacgcagggg tttaatcctc atccttatat cacgtttgct ctgccgctgt
      241 cgctttttta tgaaagcctt tgcgaagtga tggattccaa actggacggg gaaatgtcgt
      301 ttgaagaaat cgcggagcgg cttaaccgac agatgcccga gggaattgaa atttatgagg
      361 tttgcgagcc ggttctgaaa gtcactgaat tggcgtatgc ctcctatcag atccggatgg
      421 agtttccggg gcttgaaaag caggaactcg aggaggcggt cggcaggctt caaaacagcg
      481 ataagattct cgttaccaaa acaaccaaac ggcatgaaat tgaaattgac ctgaaggaga
      541 ttttcactca ggcgaagatt tcagccggca ccggttcgct tctgatcgaa gcaacggttc
      601 cggcgtctcc tcaggaaaat ctgaatccgt cctgcctgct ggcggcgatc gaaaaatacg
      661 cgggccttac cccggaaatt gaatacatta cgaggacggg aatctataat aacggtatgg
      721 aaatattccg ctgacggatc tgcgccaaca tcttgcaagt tggccggtat tgtggtatga
      781 taataaggca tttgaatttt ccgccggggc ggcgctggtt cggatactcc gaaaggtatg
      841 gaatttccgc gcggcctgct ggtggggttt aattgccggg aaaccggaaa ttaaaacgga
      901 cagtcctctg ccggacgtga acggcaagaa tgtctctatt ggaaggagag ctgattatgg
      961 acgcaatgaa acattttgta aacgcccaga tgaaagaaag catccctgag tttcatattg
     1021 gtgacacagt caaggttcac gtaaagatca aggaaggcga gagagagagg attcaggtct
     1081 ttgagggaac ggttatcgcc cgcaaaggcg gaggaatctc cgaatccatt accgtccgcc
     1141 gcgtatcgta cggcgtcggc gttgagaggg tcttcccgct gcattcgccg aaagtcgaca
     1201 agatcgaaac aatccggcac ggcagagttc gcagaagcaa actttattat ctgcgcgaca
     1261 gagtcggaaa agcggcgaaa gtcaaagaac aaatacggta aaaaaaaggg acttatgtcc
     1321 cttttttgct atttttggat tttcacgcgg tttgacggat caatcggcca gccggcgggg
     1381 aaagtccgca gtcggaattg gaacaaccga aacacggcgg tttgccgccg tttgaatcaa
     1441 tgcggatgaa ggtgtcaagc gcatgagtga tataaacatt cagccggacg gaagtgcggc
     1501 gccacaggga tatagcctga aaaatgatgt gttcgaatgg atggaagcga ttgttttttc
     1561 gctttcgttt gttgttttac tgtttacgtt tgttttccga atcgtcggcg tggatgggac
     1621 atccatgctt aacacactgc aaaacggcga tcgggtggta atttcagata tcaactatac
     1681 gccgaaacga ggcgatatta tcgttctgtc caccaaagcc gttgagaagc cgatcatcaa
     1741 acgggtgatc gccaccgaag ggcagcgtat tgatattgat tacgtcaccc atacggtaac
     1801 tgtcgacggt aaggcgctca gcgagccgta tatcgccgag cccacggttt ttcagggtca
     1861 aaatccggtg agcctgccgg ctactgttcc caaaggccat gtctttgtca tgggcgacaa
     1921 ccgtaactac tcgctcgaca gcaggagcag ctccatcggc atgattgacg accgttacat
     1981 cctgggcagg gcggtattcc gcatcttccc gttcagcggt ttcggatttt taaaatagaa
     2041 gcattggaat ttggaaagag tgggaagtta tgacagagtt gccgtcgatt cagtggtttc
     2101 cgggtcatat ggccaaaaca agaaggctga tcaaagagag ccttcatctc gtggatatcg
     2161 tcgcggagct gacggatgcc agagttccgg aaagcggcag aaatcctgag ttggatgcac
     2221 tctgcgtatc gaaaccgagg attcttctgc tcaataaaag cgatatggcc gatgagaata
     2281 ttactggaga gtggctgaca tattacaggt caaaaggata ttacgccctc agcgtcgact
     2341 gcaaaaacgg ccggggtttg aaccgcttta tgccgctgat ccgggagatt cttcaggaga
     2401 agctggagaa gctggcggac aagggcatgt ccggcaggac actgcgggta atggtcgccg
     2461 gcattcccaa tgtgggaaaa tcgaccttta tcaacacaat gtcacattcc aaaagagtca
     2521 aagtggaaga ccgccccggc gttacaaggg gaagacagtg gatttcgctc ggctccggcg
     2581 tggagatgat ggatactccc ggaatcctct ggccgaagtt tgaagacccc aaagtggggg
     2641 aaaaactggc gtttaccggc gcggtcaagg acgacgtaat tgaccgggaa ctgctgtgcg
     2701 cgcggctgct ggaggttttg gcgcaatctt attccgacga gctccgcgcg aggtataaac
     2761 tcagcggccc tctgccggaa gacggcaccg atctgctttc gatgatcggc agaaaaaggg
     2821 gaatgcttat ctccggcggg gaagtggacg gcgagcgcgc gtccgccatg gttttggatg
     2881 aattcagaag tggaaagctt ggcagaatca cgctggagaa accgtcggat catacagaaa
     2941 acgctgaaac cggatacgag gcttaaaaat ggaatggttt tattatgaac atatggcccg
     3001 gcgcgaaagc gccggtttaa tctgcggcat tgatgaggcc ggcagagggc cgcttgccgg
     3061 cccggttttc gcggccgcgg taattttacc ggatgatttg gaaattgcgg gtatcaatga
     3121 ttcgaaaaaa ctatccgcca aaaagcggga agcgcttttt gaagtaatta cgaaaaaggc
     3181 catagattac tttgtcgcgc aggcaagcga ggcggaaatt gatacataca atattttaaa
     3241 cgcgacctat cttgccatga aacgcgcggt ggaagggctg aaaaccaaac cggattttgc
     3301 tctggtcgac ggtaaccgcg acccggttct cggaatcccg acgacctgta ttgtcggggg
     3361 agacggtaag agcgcatcca tcgctgccgc gtcgattctg gccaaggtaa gcagggaccg
     3421 ctatatggaa gagcttgatc ggaaatgtcc ccagtatctt ttcgcaaagc acaaaggcta
     3481 tcccaccaaa cttcattatg aaaaaatcct gcaatacggc gtttccgagt accacagaat
     3541 gagttttctg aaaaatcttg cggaaaagag gctgtcgttt gaacaaaaat gacaccggcc
     3601 ggctgggcga gcttgccgcg gcccgatacc ttcgcaaggc aggctgcgag attctcagcg
     3661 ccaattacag gacacgattt ggtgagattg atctgattgc ctccgacggc agatttctga
     3721 tttttgcaga agttaaaacg agaagggaaa attcactttt ccttccccgc gaagcagtcg
     3781 atcagaaaaa gcaggaaaaa attatcgccg cggcaaagct ttacctctcg gtcaatcaaa
     3841 cgaaacttca gcccagattc gacgtgatcg aggttgttac cgccgccggc ggtgagtttc
     3901 aggtgctgtc aattcatcag attgaaaacg cctttacgct gtaaaagcgg aaatttgatt
     3961 cataaagggt ggtgtaatca tgcgttattt tgacctgcac tgtgatacga tcacgaaatg
     4021 cagcgataag aacaaagaac tctttcacaa tgatcttcat atatccctcg aaagagccgc
     4081 cgggtatgcg acctgggcgc agttttttgc cgtctggact ccggataaga caaacgggga
     4141 cgaggccgaa aagcggtttt ggaaaactta tgctgctttt accacacaaa tcgggaagaa
     4201 taaaaatctt attgattttt gcggaaatgc aaaggatctg gaacaggctg ttgcggcggg
     4261 taaacagatc gcgatgctgt cgattgaagg ggcggtatgt ctcaacggca ggctggagct
     4321 gattgacgaa ttttatcaca aaggagtccg gctgctgtcg ctgacctgga acgaccgctg
     4381 tatcacagcg gatggctgca tgacgcccga cgcgcagggg ctgacgccgt ttggcaggga
     4441 agtgatccgc cggatgggac ggcttggtat ggtggctgat gtatcccatc tgtcggaaaa
     4501 gggattttgg gaaattgccc gtttaaccga caggcctttt gttgcttccc attccaattc
     4561 cgcggcggtc tgcgcgcaca agagaaatct gaccgacgat cagctcaaag agatcgtaaa
     4621 ccgcgaaggg ctggtcggcc tgaatcttta cacggaattt atccgcaggg gtaaaccacc
     4681 ggtgcgggtt gaagaccttc tcagccacat tgaccatatc ctgtcgctcg ggggcgaaaa
     4741 tgtgctggcg attggctcgg attttgacgg agccacaatg ccgcagggaa tcaaaggcgt
     4801 tgaggatatg gcgaaacttc acggaatgat atccgaaaaa ttcggagctg acacggccga
     4861 taagattttt tttgaaaatg cctttcgatt ttttaaaacg gctttgacag actgtgaatc
     4921 ttgtaataat ataatagagt tataacagcg gcggttattt tgcggtcact cggcagacgg
     4981 cccgcgtggg tcggtaggcg gattgggttt cttcacgctg atcgggtgca aaatgattgc
     5041 tgcgacacaa tgagtcggcc ccggtgtgcg tggctgacca aggggagtga aactcctcaa
     5101 atctatgttt ttggcaagag ctaaaggaga gtcagtaatg aattataaaa gcaccaggaa
     5161 cagcggaata tccgtgacgg catcctccgc gatactggaa ggaatttcgg cggacggagg
     5221 gctctatgta ccggaaagca ctccctggct ttcttacaat gacataaagg ctttgaccga
     5281 ttccgactat gtatcgaggg cggcggacat cctcgggcgc tttttaaacg attttaccaa
     5341 cagcgaggtt atggattgcg tttccagagc ctacaccagg gaaaaatttg aaacggacag
     5401 tatcgctccg atctatatgc ttgaaaacgg ggtctatctg ttggaattgt ggcatggccc
     5461 cacctcggcc ttcaaagata tcgccctgca gcttctgccg tatctgatga aggtttcggt
     5521 atccaaagcg aaaaccgaaa aaaccgtcgt gattctcgtt gcgacctccg gcgacacggg
     5581 caaagccgcg ctcgagggtt tcaaggatgt tgacggcaca aaaatcatgg tgttttaccc
     5641 ggaaaacggc gtcagcccga tgcaaaaaag gcaaatgatt acgcaggaag gcggaaatgt
     5701 tgccatctgt gcgattaaag ggaattttga cgatgcccag agcgcggtaa aagaaatttt
     5761 tgccgacgcc gatatccgcg ataagctttc cgaaaacaat atgatgttct ccagcgcaaa
     5821 ctccatcaac tggggaagac tggtgccgca gattgtttat tatgtttctt cctattgcga
     5881 cctgatcaag aataataaaa ttacctgcgg tgagcaggtg aactttgtgg ttccgaccgg
     5941 aaatttcgga aatattttag ccgcttacta tgcgaaaaag atggggatac cggtcaataa
     6001 gctgatctgc gcctcaaacg ccaataatgt tctggccgat tttatccaaa ccggcgttta
     6061 tgatcgcaac cgtccgtttt acacgaccat ctccccgtca atggatattt tgatctccag
     6121 taatctcgaa cgtcttctgt tcgatctttc gggctgtgac gatgcaaaaa tccgggaatg
     6181 gatgtcccag ctggccgaaa ccggccggta tgaggtcgac gatacggtta aaaacagcct
     6241 tcatgaacat ttttggtcgg gtttctgcag cgacgacgac acaaaggcgg tcatcaaaga
     6301 gatttatgca aaatacgctt atgtctgcga tccccatacc gcggtggctc ttcatgttta
     6361 tgaacagtat aaaaagaaga cttctgatac aacgaagacg ttgattgcct ctacagccag
     6421 cccgtttaaa ttcgcaccga atgttcttga ggccatcaat ggggaaagcg gaaggctcgg
     6481 cgagcgggaa atgctcgaaa aagtcgcgga gcttgccatg tgccatattc ccaaacaaat
     6541 cgcgggcctt ttcagtaaac cggaaagatt ccgcgatgaa acctgcaaaa agacagagat
     6601 gaaagaggaa atcattcgga gtctgggcct ttaacaaaag cacgccgcag aatttctgcg
     6661 gcgtgtaagc gtcggctatt tcggctcaaa cgtggagcag acggtttccg tgctgtttga
     6721 agcgtaagtc gggccgactt ttatttcgtc ggcggcgcat ttgcccttgc ggtcgtgata
     6781 aacgcaattg gtgacttcgc agtgtatgcc tcttacatgt tcgtttttac ctattttatc
     6841 cgcaatctga ttgatgaaat tcatttgccc tcatcctttc tccggcagtg ccgaaatgtt
     6901 ttgggcgcga aacacaatga tgaatttgaa caatgttgcg tccaaagcta tttttacctt
     6961 ttatgacgtg attatcgtat tttaaatctg gaaaaggtga cgccatgtct ctttacgcga
     7021 tcggtgatct tcatctctca ttcggatcgg acaaaccgat ggatattttt accggatggc
     7081 aggattatgt ttcaaaaatc aaaaaaaatt gggaaaatat cgtaaaagaa accgacacgg
     7141 tggtgattcc cggagatgtc tcatgggcga tggattttga ttcactcagg cctgatttcg
     7201 attttattga cagactgccg ggcagtaagc tgatcatcaa aggcaaccat gattactggt
     7261 ggtcgac
//
LOCUS       DATALY010000198         4658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_217710,
            whole genome shotgun sequence.
ACCESSION   DATALY010000198 DATALY010000000
VERSION     DATALY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217710"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /gene="rpsR"
                     /locus_tag="VHR42_05765"
     CDS             <1..121
                     /gene="rpsR"
                     /locus_tag="VHR42_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX3026717.1"
                     /translation="ERAKILPRRVTGTCAHHQRELTIAIKRARHIALLPYVSD"
     gene            complement(211..696)
                     /locus_tag="VHR42_05770"
     CDS             complement(211..696)
                     /locus_tag="VHR42_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026718.1"
                     /translation="MMPFRKGIAVLAASLTLTGFSAVAAKTPDTSSPDSSSAPSSSMQ
                     NECPKMHPGFGHHMAMDELSRITGISMEDLHDKYPQKTPWQIAKSMGKLEELKSAFLT
                     QNKELLGKMEQEGKLTAEESKKVYEDLQTRVAAIDGTNVVILGAPKYKPEMKNQSGQG
                     K"
     gene            complement(1072..3048)
                     /locus_tag="VHR42_05775"
     CDS             complement(1072..3048)
                     /locus_tag="VHR42_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026719.1"
                     /translation="MNKMQNDNRNREKASRIHQSISAWEAEAMQSRDQGQPAQKASPD
                     SDDGQTVINREAFSAVKHGRRAVESHKSPTESGEQINYESKVYDCDYQNEEILKRQES
                     LRFSLLIRMLTGLFLALVLCWLDVAAVYGYPLPPFVSFSDHYFYFIGVNAVLLALTAV
                     AGFPVVAPGIASLFTFGENSNSITALAFLGALAGNVGLLFVPVSGHVAVYSSAAAMCI
                     ALSLLGKYLNVKRVIRNIQYASGQHSFYSLDAIDDYDTAVSLSRGVAADTPFVCTTEK
                     TRFLDNFIEHSYSCGPKDKAARILAPVAFLAALAAAGGVYFVKKDLSVSLIAFSTICC
                     VGSPVLFELGNSLPFYRAGKKLNKKKAMITGYEAIDDFSLVNVVVAEDNMLFPAGSVT
                     VHGIKTFSGYKIEQAILNAASIVSAANSPLAPAFLSLIEQDGKSLLKKVDGLSGEDEM
                     GICSWIENQRMLLGNRELMRRYGVDAPSRDYEAKYLGEGRDLIYFAVGGLLSAMFILE
                     YNSHKEVAAAVRRLQRCGVSLLVKTFDPNINSTLLSDKLKINYGSVKIMSTKERELLN
                     SQFSDTAPSSDAEIAFSEGFRSYSDAVISAIKLRGTFSVCNFLQIFSAVLGIFFMIFT
                     SFTSGINEITPVHLLAYQLLWAVPVLLINLMRKH"
     gene            complement(3104..3862)
                     /gene="rlmB"
                     /locus_tag="VHR42_05780"
     CDS             complement(3104..3862)
                     /gene="rlmB"
                     /locus_tag="VHR42_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX3026720.1"
                     /translation="MQTERSGLHEEVIAGRNPVIEALKSGRPVDSVMIVRDNKQGPVL
                     KIIALCREKSIPIKEVSPVKLDSLCPGVNHQGVAAVAAAHEYAEVEDILELARKRDEP
                     PFLIVADDIEDPHNLGAMIRTAEAAGAHGVIIPKRNAAGLTSTVDKASSGALEYVPVA
                     RVANLASTIDELKKQGIWVYGADMEGQPYYKTDFGGAVAIVIGSEGKGISRLIKEKCD
                     FLVSIPMRGKISSLNASVACGVLLYEVTRNRLQK"
     gene            complement(4086..>4658)
                     /locus_tag="VHR42_05785"
     CDS             complement(4086..>4658)
                     /locus_tag="VHR42_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3026721.1"
                     /translation="MEVSFVSVDLSSMLKKFDAGKADAVIGDFPAGGSKNVLYTRPYL
                     KCGQVIVVNSGSTIQNKNGLVGKAVGVLAGSAAAKVLSRDDIVLKIKDRAPVNYYDYI
                     SAFMDLDASKLDAIALDETAGRYYVSQKETQYRLLGDRFPDSEEYAVAVRRNDGRLRN
                     QIQDAMNQMTKDGTTAQISKKWFGEDLTIH"
BASE COUNT         1176 a         1237 c         1021 g         1224 t
ORIGIN      
        1 cgagcgggcg aagattcttc cccgtcgtgt gacaggcacc tgcgcccatc atcagcggga
       61 acttaccatt gctatcaagc gcgcacggca tatcgccctg ctgccttatg tgagcgacta
      121 aaatgaaatc ttaaaacccc cctctatgat atatcataga ggggggtttc gatatcgcat
      181 taaaaattcc tttttataaa gcggaatgca ctatttgcct tgaccgcttt gatttttcat
      241 ttcaggttta tatttgggtg cgcctaaaat aacaacattg gttccgtcga tagccgcaac
      301 ccttgtttgg agatcttcat agactttttt gctttcttca gcagtcagct tgccctcttg
      361 ttccattttg ccgagtaatt ccttattctg tgtcaggaac gcgcttttta actcttccag
      421 tttacccatg ctctttgcga tctgccaggg tgttttctgg ggatacttat catgcagatc
      481 ttccatactt atgccggtaa tgcgggacaa ttcatccatt gccatatgat ggccaaagcc
      541 cgggtgcatt ttggggcatt cattctgcat tgacgaggaa ggtgcggaag acgaatccgg
      601 gctgctggta tccggtgttt tggcagcaac ggcggaaaat ccggtcagtg tcagactggc
      661 cgcaagaaca gcaattcctt ttcgaaacgg catcataaac agtcacctcc tgtaaaacga
      721 cttgcattcg cttttgccgg cattttaccg tcaaaaaacg aaatgcacat taaaagtata
      781 accaaatcta aattgattaa aataataggt ccaatccaat attttaaaga ttcgtgttta
      841 aatttccaaa aacgatcatt tccgtatttc atcagaaagt ctgtatcctg cgctttctat
      901 ttcttgcctt attctgcggt atgaccgctt tgaaaaagag ttttggaaag cttggattcc
      961 aagtggcaaa attaagtttt taatctattt ttgtaacgat aaaaggccgt ctcgtttgag
     1021 acggcctccg cttgttatgg cccaccgcga aacacttatt atcgcggttt cttaatgctt
     1081 tctcatcaaa ttaatcagca gcaccggaac tgcccaaagc aattgatagg cgagaaggtg
     1141 aaccggggta atctcattaa ttccggacgt aaaagaagta aaaatcataa agaagattcc
     1201 cagaaccgcg ctgaaaatct gaaggaagtt gcagacgctg aaagttccgc gcagtttaat
     1261 tgccgaaatc accgcgtcgg aataagaccg gaacccttca ctgaatgcga tttccgcatc
     1321 ggaactcgga gcggtatcac taaattggga gttgagtaat tcccgctcct ttgtgctcat
     1381 aattttcacg ctgccgtaat tgattttcag cttatccgag agcagtgttg agttgatatt
     1441 gggatcaaag gtcttcacca gaagagaaac accgcagcgc tgcagtcttc ttaccgccgc
     1501 agccacctct ttatgagagt tatattccaa gataaacata gcgctgagca ggccgccgac
     1561 cgcgaaataa atcaggtcgc gtccttcgcc cagatatttt gcctcataat cgcgggaagg
     1621 cgcatccacg ccgtaacgcc gcatcagctc ccggttgccc agaagcatcc tctgattctc
     1681 aatccaactg caaattccca tttcgtcttc gccggaaagg ccgtcaacct ttttcagaag
     1741 actctttcca tcctgctcga tcaggcttag aaaagccgga gccagcgggc tgttcgccgc
     1801 actgacaatg ctcgccgcat tcaggatggc ctgttcgatc ttatagccgg aaaatgtttt
     1861 gattccatgc actgtgaccg agcccgcagg aaacagcata ttgtcctccg ctaccacgac
     1921 attgacaagg ctgaaatcgt ctatggcctc atacccggta atcattgctt ttttcttatt
     1981 gagctttttc cccgcgcggt aaaacggaag cgagtttccc agctcaaaca gtaccggtga
     2041 tccaacgcag caaatggtgg agaaagcgat cagggaaacc gatagatcct ttttgacaaa
     2101 atagacgccg ccggccgccg ccaacgccgc gagaaatgcc accggtgcaa ggattctggc
     2161 cgccttatcc ttcggcccgc aggaatagga atgctctata aagttatcta aaaatctggt
     2221 tttctccgtc gtgcagacaa aaggcgtatc cgccgcgact cctctggaaa ggcttacggc
     2281 ggtgtcatag tcgtcaattg catccagaga atagaacgag tgctgcccgg acgcatattg
     2341 gatattccga atgacccttt taacgttcag atattttccc agcaggctca acgcgataca
     2401 catagccgca gcggaagaat aaaccgcgac atgacccgaa accggaacaa aaagcagacc
     2461 gacgttgccc gcgagcgctc cgagaaaagc aagcgccgtg atgctgtttg aattttcccc
     2521 aaacgtaaac agactggcaa ttcccggggc aacgaccgga aaacccgcca cagcggtcag
     2581 ggcaagcaga accgcattaa caccgataaa gtaaaaataa tgatcactga aacttacaaa
     2641 aggcggcagc ggataaccgt aaacagccgc aacgtccagc cagcagagca ccagggccag
     2701 aaacaaaccg gtaagcattc ggatcagcag cgaaaatcga aggctctcct gccgtttgag
     2761 aatttcttca ttctgatagt cacaatcata aacctttgac tcataattga tctgttcccc
     2821 tgattcggtg ggtgatttat ggctctcaac cgctctgcgg ccgtgtttga ccgcggagaa
     2881 agcttcccga ttgatgaccg tctggccgtc atcggagtct ggagacgcct tttgtgcagg
     2941 ctgcccctga tcgcgggatt gcattgcctc cgcttcccag gcgctgatcg actgatggat
     3001 cctgctcgcc ttttccctgt ttctattatc gttttgcatt ttattcattt gactgcttca
     3061 ctccaccaac aatattgtgc tgaaaatgaa ttagcatccg gatttatttt tgtagcctgt
     3121 ttctcgtgac ctcgtacagc agtacgccgc aggctaccga ggcgttcagg gaggatatct
     3181 tccccctcat gggtatggaa accaggaagt cacatttttc ctttatgagg cgggaaatcc
     3241 ccttgccttc ggagccgatt acgatggcaa ccgccccgcc aaaatccgtc ttataatagg
     3301 gctgcccttc catatcggcg ccatagaccc agattccctg ctttttcagt tcgtcaatgg
     3361 tcgacgcgag attcgccact cttgccaccg gaacgtattc cagcgcgccg cttgaagctt
     3421 tgtccaccgt ggaagtcagg cccgccgcat ttcttttcgg aattatgaca ccgtgggctc
     3481 ccgccgcttc cgctgttcgg atcattgccc ccagattatg cggatcctcg atatcatccg
     3541 caacaatgag aaacggaggc tcgtctcttt tgcgcgccag ctcaagaata tcctccacct
     3601 ctgcatattc atgggcggcc gccaccgcgg caacaccctg atggttgacg cccgggcata
     3661 gagaatccag tttcacgggg ctgacttcct taatcgggat ggatttctca cggcaaagcg
     3721 caataatttt taacaccggc ccctgtttgt tgtcccttac gatcatcaca ctgtcaaccg
     3781 gcctgcctga tttcagcgct tcaatcaccg ggtttctgcc cgcaataact tcttcatgaa
     3841 gacctgatct ctctgtttgc atatttgccc cctgatcttc gacaatatta tctataactg
     3901 cctattttat taatttatca aaaagtattc gtctcttttt ttaattatac cataaaaaca
     3961 ataaaatgaa acatgtctta gttaaaatct acaaaaaacg gccgccagcg gtaacaggat
     4021 acaaaacgca aaaaaggcgc cggtcttcag gccgacaccg tttttcactg tctttcacca
     4081 ttcgtctaat gaatcgtcag atcttcacca aaccactttt ttgaaatctg agcggttgta
     4141 ccgtccttcg tcatctgatt catcgcatcc tgtatctggt tgcgcagtct tccgtcattt
     4201 cttctcaccg ctacggcgta ttcttccgaa tccgggaacc tgtcgccgag caaccgatac
     4261 tgcgtctctt tttgagaaac ataatatctc ccggcagttt cgtccagagc aattgcatcc
     4321 agtttggaag catccaggtc cataaaagcg gaaatataat cgtagtagtt caccggagca
     4381 cggtctttga ttttgagaac aatatcgtcc cgtgaaagca ccttcgccgc ggcagatcca
     4441 gccagcacac cgaccgcctt tcccacaaga ccatttttgt tttgaatggt cgagccggaa
     4501 ttaaccacaa tcacctgccc gcattttaga taagggcgtg tgtagagtac gtttttgctg
     4561 ccgcctgccg gaaaatcccc gatcacagcg tccgctttgc cggcatcgaa ttttttcagc
     4621 atggaagaca ggtcgaccga gacgaaagag acttccat
//
LOCUS       DATALY010000199        13307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_560325,
            whole genome shotgun sequence.
ACCESSION   DATALY010000199 DATALY010000000
VERSION     DATALY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13307
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560325"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VHR42_05790"
     CDS             complement(<1..568)
                     /locus_tag="VHR42_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3026722.1"
                     /translation="MSEKIIQLNEGIIKEELKDLVRNSVEETLNGLLDKEAAELTNAA
                     KYERTETREGYRSGHYNRNLQTTSGEVNLKVPKLKGVPFETAIIERYRRRESSVEEAL
                     IEMYLAGVSVRRVEDITEALWGTKVSPGAISELNKKAYVHIEDWRNRPLQGGKYPYVY
                     VDGIYLKRNWGGEYENVSILVAIAVNEDG"
     gene            complement(704..1546)
                     /locus_tag="VHR42_05795"
     CDS             complement(704..1546)
                     /locus_tag="VHR42_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3026723.1"
                     /translation="MPNETINRKPQEYFSTGEFAKLCGVNKRTLFHYHDIGLFYPAMI
                     DQNGYRYYSYHQFNVFLIITVLKELDVPLKEMKVYLDERTPDRLLELSKQKIVEIDRE
                     IEKSLQIRHFLEETIAFTNKGMSADPDRIIVEEQEEECMIRSALLSDENSKDYIRWML
                     EYNKFTNSTRSKSSSFVGAMISKNKIMNGDFQGGSYLFAKTGSKNPTPSMAVKPKGLY
                     AVAYHRGSYETINKTYEKLLMFLQENQLDLGDFAYEEYLLDEVAVKDKNDLVTQITVA
                     VEKR"
     gene            1806..3155
                     /locus_tag="VHR42_05800"
     CDS             1806..3155
                     /locus_tag="VHR42_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3026724.1"
                     /translation="MKLTDGTNKAILKNVLSYALPSVIAMLVTSAYVVIEGIFLGRGV
                     GANALAAVNIAFPAMVLINAVIIAISVGGANLMSIRLGEGKPEDANNIFMQCLVAIIA
                     ISVLFSLLGIIFLRQISVLLGANDVLLNNVMVYLRYALIFSPVFALSNCLNTFIRNDG
                     NPRLSMYSMVIGSIAIAIFIYMFVFLLKSGIAGAAIAGGLGDMFTIVIVSVHFIKKKG
                     ILRFSKPVFKKADIIKISGLGLPSMMTELAMSFSTICFNLVIIRRIGAIGVTAYSIIT
                     YITSFVILILMGISQGLQPILSFNHGAKEVKTVQKTYRIGILLALAVSILTILMLLIW
                     GTNIIMVFNDDSQKLINLTRSAILCFCAGYIPAAINIVNITYYQSTERPKLSSAVSIL
                     RGFAFVLLGLFILPPLIGNIGIWLTSVFGESLTLIITLLLGKKLKSQILSVEIIGQK"
     gene            3363..4574
                     /locus_tag="VHR42_05805"
     CDS             3363..4574
                     /locus_tag="VHR42_05805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3026725.1"
                     /translation="MSREKQLKMKKVTTEHLKQYNQLLRYVFQVTDNELHKIGWEERD
                     MVHAKTPVLEQADVLGWFDGEKLVSQVAVYPFQVRIFNRTYDMGGLTGVGTFPEYSNQ
                     GLMHKLLLQALTNMREKKQSISYLYPYSIPYYRRKGWEIISDKITFEVNDYQLPKSKQ
                     VSGEVERVDTDSEDVKAAYARFANQSHGAMLRNDLAWNEYWLWDYDDLTAAIYYNENK
                     EPDGYILYWIADEVFHIKDMIFINEEARSGLWNFISAHFSMISKVIGDIYTDEPQAFL
                     LEDADIKETIAPYFMARIVDLEQFINLYPFKPDNQERVWTFTLDDPLLSWNQGTFTLR
                     IHPDGKGEVVRTGEHCGSRIDIQTLTTMLLGYKRPDYLHKIGRLSCSPKIVDLLEDSI
                     EQQTPYFSDYF"
     gene            4697..5455
                     /locus_tag="VHR42_05810"
     CDS             4697..5455
                     /locus_tag="VHR42_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEX3026726.1"
                     /translation="MQSQITKELISERAFQLGANLCGVGDLTADQAFIQENYGDYCAH
                     FPRAVSVAIFFPKEIIHEQQVGPTHSYEYFYNTLNRQLDNIGLIISVMLQNAGYRAYP
                     VPTSDYRQDARGENLQKLVAENHTATLPRIGTERMGMFSHRLAAREAGFGWIGKSCNL
                     INPTVGPCLRLTTVLTDAPLPADSPVPNRCGSCTRCRDACPAKAISGRLFNPAESVTD
                     RLDRGKCYGQLQAMGQVFGQSTCALCLAVCPWGH"
     gene            complement(5607..7334)
                     /gene="aspS"
                     /locus_tag="VHR42_05815"
     CDS             complement(5607..7334)
                     /gene="aspS"
                     /locus_tag="VHR42_05815"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX3026727.1"
                     /translation="MLRTHHCNELRQEHIGTKATLAGWVDTIRDHGSVKFLDLRDQNG
                     VTQIVFHDDSMLEGINRETVISATGDIRARDNDTINPKLDTGMVELHVDSISVLGTCK
                     NVLPFEIRDSVSTREEIRLKYRYLDLRNPAIHQNIVLRSKVISFLRKRMEELGFPEIQ
                     TPILTASSPEGARDYLVPSRKHEGKFYALPQAPQQFKQMLMVSGFDKYFQVAPCFRDE
                     DARGDRSPGEFYQLDFEMAFAEQEDVLAVAETVLYDAFAKFSDKHISPAPFRRITFHD
                     AVMKYGSDKPDLRNPLTICDLTEFFSHVEFAAFKNKPVRGIVADCSGRPRSFFENSLK
                     YATSIGMKGLGYITLTEGNFKGPIEKFLSPDQKKQLITDCAIQEGQTLFFISDKKQAV
                     NKLAGQIREWLGENLELIDKNAFEFCFIVDFPMYEYNPETNKIEFTHNPFSMPQGGLT
                     ALETKNPLEILAYQYDVVCNGVELSSGAVRNHSPEIMKKAFSIAGYSEEELEKRFGAL
                     YTAFQYGAPPHAGMAPGIDRIVMLLAGEEAIRDVIAFPLNGNAQDLMMGAPCEVTATQ
                     LREANIKIR"
     gene            complement(7892..8950)
                     /locus_tag="VHR42_05820"
     CDS             complement(7892..8950)
                     /locus_tag="VHR42_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026728.1"
                     /translation="MNTIIKSALNQIHAEDKLVEKTEKALRDILEKQKNDKTIILKRR
                     SVFSMKKFVFAACAAVLVCGLSIGGYAYYKTPVAYLSLDINPSVELGVNTFNQVVSVT
                     GYNADGKTILAGKNVVNSNIKDAVEELIASASKNGFINSDGSTIVSVTAETDNAAAAS
                     ALEKNAQQGADDAIKSSGDTAVVYKDNVALSRRDEARKLGITPGKLNLIQKLQALDPT
                     ITVDQYKDAKVTDIMKKIIALRSLNSTDNSDSETQDIESAVKQSNKNADNTHSHKNEN
                     NSSSSTVTNSSSTKNNDNNKQNDYNSSAVTSSTKGNGNSDAQNNRNNNRNSASTASST
                     KGTGKSQEHGSNQGKQAN"
     gene            complement(8934..9434)
                     /locus_tag="VHR42_05825"
     CDS             complement(8934..9434)
                     /locus_tag="VHR42_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3026729.1"
                     /translation="MEKASFGTNDVVIKALQKHSDMVRRICFMYLRNHTDVEDVFQEV
                     FLKLLQSEIAFENDEHIKAWLCRVTINKCKDVCKSFFKKNICSIEDMELSFEDKKEND
                     VMAAVLSLPSKYKDVIYLFYYEEYTVPQMAKLLHQKENSIYSNLHRARKLLRQKLGGS
                     DHEYNY"
     regulatory      complement(9588..9668)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(9949..11115)
                     /locus_tag="VHR42_05830"
     CDS             complement(9949..11115)
                     /locus_tag="VHR42_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026730.1"
                     /translation="MQVFKVYFKILKSNLPVMMIYVGIFIALAIGLSSFGGSNSALNF
                     TPTKTNIAFINNDHPSPLTNSLKSYLSRNANIVPLTDDTQDLQDALFYNRVDYIVRIP
                     KGFTQNFLSGKNDIRLEKTSIPNSVSSVNMDFLVNRYLNTASLFEKSLPGVSQTQLAT
                     DISGNLKTAADVHLTSFGSPAEKSGFIYYFTYFAYVIAAVLILGVSSIMTVFNESDLK
                     SRNLCSPVKAVHINMQLLLGNLTFSFVTWALFMGLSVLIYRESLPNRHFILLCLNSLV
                     LMLAWLSISFLVGNLIRSRSAQSAAANVLALGSSFIGGVFVPQALLGKTVQTIASFTP
                     TYWYIKALNDINSLVVGSAANLMPIFYCMLIQLGFAVAIVAVSLVIGKQRKTSY"
     gene            complement(11102..12253)
                     /locus_tag="VHR42_05835"
     CDS             complement(11102..12253)
                     /locus_tag="VHR42_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3026731.1"
                     /translation="MFLHVFVNRFKCLVRDRQLLFWTLLFPIVLATFFHMAFSNLQSS
                     QNFENIKIAVVNDSNYQNNSAFRSALAAVSTDVKPGQSRLFVVTPAAKQRAVDLLKNS
                     KIDGYFQLSGSGKIEVFVKESGINQTILKEFADDYVQTSAAAKTILSKNPAAIQNFTA
                     NLSQMDYLKEVSPTKAAPNDILNEFYSLIAMACLYGGFWGIKEVVAVQADLSAQGARV
                     SVAPVHKLKIFSSALCAATAVEFTSAVVLIAYLNFVLKVDFGNQIGYVLLACLAGCFT
                     GVTMGALIGAVIKKGEGIKVALLIFLTMALSFLTGLYGAPSRYTIIKAFPPIAWLNPA
                     NVITDAFYSLYYYNTHTRYFTNIGVLFGMSTVFIFIVSVVLRRQKYASL"
     gene            complement(12267..13205)
                     /locus_tag="VHR42_05840"
     CDS             complement(12267..13205)
                     /locus_tag="VHR42_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026732.1"
                     /translation="MVVKVENLVKRYGDLIALDHFSLDVDEGEVFGLLGPNGSGKTTA
                     INCILSLLKYDKGSIEVFGRPMSPNSYAIKRDIGIIMQNVAVFSELTVYENINYFCGL
                     YITDKAKRAELVDEAISFVGLDEYRKFVPKKLSGGLLRRLNIACGIAHKPKLIILDEP
                     TVAVDPQSRNNILEGILKLNRQGATVIYTSHYMEEVEQICSRIAIMDKGKIIASGTND
                     ELKGMINTGEKIIAEIFNFHNSLLEDLRSLPNISSVDYNESENRVVIKSNRGKNNLTD
                     VLEYLKAKEIAYGKIYCELPTLNDVFLEITGKELRD"
BASE COUNT         3356 a         3039 c         3024 g         3888 t
ORIGIN      
        1 acccgtcctc gttgaccgca atggcaacaa ggatggatac gttttcgtat tctccacccc
       61 agttcctttt caggtatatt ccatccacat aaacataggg atatttaccg ccctgtaaag
      121 gacgattgcg ccagtcttcg atatgtacgt atgctttctt gttcaattca ctgatggctc
      181 cgggagaaac cttagtcccc catagggcct ctgtaatatc ttctacccta cgaacggaaa
      241 caccggccag atacatttca atcaacgctt cctcgacgga gctttcccgt ctacggtaac
      301 gttcgataat tgctgtttca aaagggacgc ctttgagttt cggcactttc aggtttacct
      361 ctccggaggt cgtttgcaga ttacggttgt aatgtcccga acggtacccc tcacgggtct
      421 cagtacgctc atacttggcg gcattggtta gttctgctgc ctctttgtcc aagaggccgt
      481 tcagggtttc ttccacactg tttctaacca ggtctttcaa ttcttccttg atgattccct
      541 cgtttaattg tataatcttt tcggacatgg tttgcagtct cctttttggt gaatgttgtg
      601 tggtaacttt attctaccaa atggctgcaa atctgtcctc cttttttatc ctttttgaat
      661 ttgcgaaact tattatacct tatcacctga acgggccgct ttttcatctt ttttcgacgg
      721 caaccgtaat ttgcgtaaca agatcgttct tgtcttttac tgcgacttca tctaaaagat
      781 attcctcata cgcgaagtca cccaggtcta attggttttc ctggagaaac attaataatt
      841 tttcataagt cttattgatc gtttcatagc tgccgcggtg ataggcaaca gcgtacaatc
      901 cctttggttt tacggccatt gaaggggtcg gatttttgct gcctgttttt gcaaaaagat
      961 acgagccgcc ttggaaatca ccattcataa ttttattttt actgatcatt gccccgacaa
     1021 atgagctgct ttttgaacga gtgctgttcg taaatttatt atactccagc atccatctga
     1081 tataatcctt actgttttca tcactcagca gagcactgcg gatcatgcat tcttcttctt
     1141 gttcctcaac aatgattcga tcgggatcag cgctcattcc cttattggta aaggcaatcg
     1201 tttcttctaa aaagtgcctg atttgcagag atttttcgat ttcccggtcg atctctacaa
     1261 ttttttgttt ggaaagttca agcagccggt cgggcgttct ctcgtcaaga tagactttca
     1321 tctcctttaa cggaacatcc aactctttta agacagtgat gattaaaaaa acgttaaact
     1381 gatggtagga ataatagcgg tatccgtttt gatcgatcat ggcagggtaa aacaggccga
     1441 tgtcatgata gtgaaaaagg gttcttttgt ttacgccaca aagctttgca aattctcccg
     1501 ttgaaaaata ttcctgtggt tttcgattga tggtttcatt cggcatttta tagttcttcc
     1561 cctcttgact gtctggttac gttatacttt attataaagc cattgaatgg aatattcaag
     1621 aaaatggact gtatcggaaa tgggggctgt tttaatcttc ccaaagccgt atcgtgaaaa
     1681 aagcggaaac aaatcatcaa caaaaaacct atcaatccgg agcgcgctgg aataaattga
     1741 agtattttta tatatgagag caaaattcgg cgttaacgag gaaagatatt cgtggaggcg
     1801 tcaaaatgaa actgaccgat ggtaccaaca aggcgatatt gaaaaacgtt ctcagctatg
     1861 ccctaccttc cgtaattgct atgttggtta cctcagccta tgttgtgatc gaagggatat
     1921 ttctcggacg gggagtaggc gccaacgctt tggctgccgt caatattgcg ttccccgcca
     1981 tggttctgat caacgcagtg attattgcga tctctgtcgg cggagcaaat ctaatgtcca
     2041 tacggctggg tgaggggaag ccggaagatg ccaataatat ttttatgcag tgtttggttg
     2101 ctattattgc aatttctgtt ttgtttagct tactgggaat catttttttg cgtcagatat
     2161 ccgttctttt aggtgcgaac gatgtccttc tcaacaatgt tatggtatat ctgaggtacg
     2221 ctttaatatt ttcccctgtt tttgctttaa gtaattgcct caacactttt atcagaaatg
     2281 acggaaaccc gaggctgtct atgtatagca tggtcattgg ctcgattgcc atcgcaatat
     2341 tcatttatat gtttgtgttc ctgctgaaat cgggaattgc cggagcggcc atagccggcg
     2401 ggcttgggga catgtttact atagtcatcg tctccgtcca tttcataaaa aagaagggaa
     2461 ttttaagatt ttcaaagcct gtattcaaaa aggctgatat cataaaaata tccggtctgg
     2521 ggctcccttc gatgatgacg gaattggcaa tgtccttttc cactatttgt tttaacctgg
     2581 taatcatacg acggatagga gcaattggtg ttacagccta tagtatcatc acctatatca
     2641 cgtcttttgt aatactgatc ctgatgggga tatcccaggg tttgcagccc atattaagct
     2701 tcaatcacgg agcaaaagaa gtaaaaacgg tacaaaaaac atatcggatc ggtattttgc
     2761 ttgcgcttgc ggtatccatc ctgacgattt tgatgttgct tatctggggt actaatatca
     2821 taatggtttt caatgatgac agtcagaagc ttatcaattt aacgagatca gcaattttgt
     2881 gtttctgcgc gggatatatt ccggcagcga tcaatatcgt taacatcacc tactatcagt
     2941 ctactgaaag gcctaagctg tccagcgcgg tatccatact cagagggttt gcgtttgtcc
     3001 ttcttgggct gtttattctt cccccgctta tcggaaatat agggatatgg ctgacgtcgg
     3061 tatttggaga atcacttacg ctgatcataa cattacttct tggaaaaaag ctgaagagtc
     3121 aaatactctc tgttgaaatc ataggccaaa agtaattttt gtagaattat gggctttggg
     3181 ccgcgggtgg taccacagta aatgggcaag actagtaata cccataggag cataagccta
     3241 ccagtaggac agtatctttc tggttaaatt ttgattgatc atttcgccgc ctggaatgga
     3301 aaccggtttg atttaataaa tatgatatta tagcagaccc taatatagta agggtggttt
     3361 atgtgagccg agaaaagcag ctgaaaatga aaaaggttac aacagaacat ctgaaacagt
     3421 acaatcaatt gctccggtat gtttttcagg tgaccgataa cgaattacac aagatcgggt
     3481 gggaagaaag ggatatggtc catgccaaaa ccccggtgtt ggagcaggcc gatgttctgg
     3541 gatggtttga tggagaaaaa ctcgtgtccc aagtagctgt atatcctttt caagtgagga
     3601 tattcaatcg aacttacgat atgggcgggc ttaccggagt aggcactttc cccgagtatt
     3661 ccaatcaggg actgatgcac aagctgctgc tgcaggcgct tacgaatatg cgcgaaaaga
     3721 aacaatcaat ctcctatctg tatccttatt ccattcctta ttatcgcagg aagggctggg
     3781 aaatcatttc agataaaata acgtttgaag tgaatgatta tcagctgccc aaaagcaagc
     3841 aggtttcggg tgaagtggaa cgtgtcgata cagacagcga ggatgtgaaa gcggcttatg
     3901 cgcgctttgc caatcaaagc cacggggcta tgctgcgtaa tgatttagcc tggaacgaat
     3961 attggctgtg ggattatgac gacctgaccg ccgcgattta ttataatgag aataaagagc
     4021 ccgatgggta tatcctttac tggattgcag acgaagtgtt ccatattaaa gatatgattt
     4081 ttatcaatga agaagcccgc agcggactat ggaattttat cagcgcacat ttttccatga
     4141 tttcaaaagt tataggcgat atctatacgg atgagccaca ggccttcctg ttggaggacg
     4201 ccgacatcaa ggagacgatt gcaccctatt tcatggcccg cattgttgat ttagagcagt
     4261 ttatcaacct ctatcccttc aagcctgaca atcaggagcg cgtatggacg tttacgcttg
     4321 acgatccgct tctgtcatgg aaccagggca ccttcacgct gcgtatccat cccgatggaa
     4381 agggcgaggt agtacgcacg ggggagcact gcggctcacg gattgatatc caaactctga
     4441 caacgatgct gcttggatat aagcgtccgg attatcttca taagatcggc cgcctttcat
     4501 gcagtccgaa gatcgttgat ctgcttgaag attccatcga gcagcagacc ccttattttt
     4561 cagactattt ttaaccgtct gcggtcggaa aattatatag tcttcataac gcgaacaaat
     4621 cagttacttt ctacaaacct ccatgatgtt ttttcaacga atcgttttat gaagaaatca
     4681 atagggaggt gttttgatgc aaagccaaat aacgaaagaa cttatttctg aacgggcgtt
     4741 ccagttgggc gcgaatcttt gcggcgtagg tgatctgaca gccgatcagg cttttattca
     4801 agaaaattac ggcgattatt gcgctcactt tccccgggcg gtttcggtcg cgatcttttt
     4861 cccgaaagag attattcacg aacaacaagt cgggcctacc cacagttacg aatattttta
     4921 taatacgctt aaccgtcagc tggacaatat cggtctaatc atttctgtta tgctgcagaa
     4981 tgccggttac cgtgcttatc cggttcctac ttctgactat cggcaggacg cccgcggcga
     5041 gaatttacag aagcttgtcg ctgaaaatca cacagccaca ctgccccgaa taggcaccga
     5101 gcgaatgggc atgttcagcc atcggttggc tgccagagaa gcaggttttg gctggatagg
     5161 gaaaagctgc aacttgatta atccgaccgt cggcccttgt ttgcggctga ccaccgtgct
     5221 gaccgacgca cctctgccgg cagattctcc tgtgcccaac cgctgcggaa gctgtacccg
     5281 ctgccgggac gcctgccctg cgaaagcaat ctccggccgc ttgttcaatc cggcggaatc
     5341 ggtgacggac aggcttgatc ggggaaaatg ttacgggcag cttcaggcaa tgggacaggt
     5401 tttcggccag agtacctgcg ctctttgtct ggcggtttgt ccctgggggc attagtggtt
     5461 accggttata tgaatcgaat tagagacaca tatattttgt gcccgccgat ttgatcggcg
     5521 ggcacatgtc tttattgcag ccgatcagag ggtctctgcc ctgattggct aaaagtttac
     5581 tgactctttt atttctaacg tccgtattag cggattttta tatttgcctc tcgaagctgg
     5641 gtcgctgtga cttcacacgg cgcgcccatc atgaggtcct gcgcgtttcc gtttaacggg
     5701 aacgcgatga catcgcggat cgcttcctct ccggccagca gcatcacgat acggtcaatg
     5761 cccggggcca tgccggcatg gggcggcgcg ccatactgaa acgccgtata caacgcaccg
     5821 aacctttttt ccagctcctc ttccgaatat ccggcaatcg aaaatgcctt tttcatgatc
     5881 tccggcgagt ggttccttac cgcacccgac gagagttcaa cgccgttgca gacaacatcg
     5941 tactgatacg caagaatctc aagaggattc tttgtctcaa gggcggtcag gccgccctgg
     6001 ggcatcgaga agggattatg agtaaattca atcttatttg tttcggggtt gtactcatac
     6061 atcggaaaat cgacaataaa gcagaattcg aatgcattct tgtcgatcag ctcgagattt
     6121 tccccaagcc attccctgat ctgcccggcc agcttattga cggcttgctt tttatcacta
     6181 ataaagaaca gcgtctgtcc ttcttgaatc gcgcaatcgg tgatgagctg ctttttttga
     6241 tccggcgaca ggaatttctc aatcggcccc ttaaagttgc cctcggtaag agtgatataa
     6301 ccgagtcctt tcattccgat acttgtagcg tattttaagg aattttcgaa aaagctccgc
     6361 ggacggcctg agcagtcggc cacaatgccg cgtaccggct tgtttttaaa ggccgcgaat
     6421 tcaacatggg agaaaaactc ggtcaggtca cagattgtaa ggggatttcg caaatcgggt
     6481 ttatcgctgc catatttcat gactgcgtca tgaaaggtga tgcgccggaa aggcgcgggc
     6541 gatatgtgtt tatccgagaa ttttgcaaac gcgtcgtata aaacagtttc ggcgacggct
     6601 aaaacgtcct cctgctctgc aaacgccatt tcaaagtcga gctgataaaa ttcacccggc
     6661 gaacggtcgc ctctggcatc ttcatcccgg aagcagggcg cgacctgaaa atacttatcg
     6721 aagccggata ccatcagcat ctgcttaaac tgctgcggcg cctgcggcag cgcgtagaat
     6781 tttccttcgt gcttgcggct cggaacaagg taatcgcgcg cgccttccgg cgaggacgcg
     6841 gtcaggatcg gtgtctgaat ttccggaaac ccgagttcct ccatacgttt tctcagaaaa
     6901 cttataacct ttgaacgcaa gacgatattt tggtggattg ccgggtttcg caggtccaga
     6961 taacggtatt tcaaacggat ttcctcgcgc gtcgaaacag agtcccggat ttcaaacggc
     7021 aaaacgtttt tgcatgtgcc caaaacactg atgctgtcga catgcagttc aaccatgccg
     7081 gtgtccagct ttggattgat ggtgtcgtta tcccttgcgc ggatatcccc ggttgctgag
     7141 atgacggttt cgcggttaat tccctccaac atggagtcgt cgtgaaacac gatctgcgtc
     7201 acgccgttct ggtcgcgcag atccagaaac ttcacacttc cgtgatcccg gatcgtatca
     7261 acccaaccgg ccagggtggc tttggtgccg atgtgctcct gtctgagttc attgcagtga
     7321 tgtgttctta gcatcattga tcctcccaaa acaaaaaatc ccggaaatta tcttcaataa
     7381 taatttccgg gacgagcatt ttcttttgcc cgcggtgcca cccgcattga tacgggaaac
     7441 ccgcatccac tttcttattc aatttccggt aaacatgaag tgttccccgg cttactactc
     7501 ttccaaatcg cgctctcaat cggtgacgcg aattctctgt tggaatttcc acgaaagcgg
     7561 agtcttcata gaaattatat gccatgtaag agaaacagtc aacaggtatt attgtagatt
     7621 aatttctgtt atggcaccgt cacatagtgt tttcttacaa attccattgg ctttgacaac
     7681 ttctttctcc cgagtttacg agaccctata tttagatatt gccaattgcc tgcgtaatat
     7741 tctgttttta agaggctcta tacacgaaca agatatttga aatgacagtg atacggaaga
     7801 gtgctggaaa tttatgttta caggactcct ggcttccgca atgggtgccg gtttgttacc
     7861 ggcaggtgga aaaagtcgct gtatggttcg ttcaatttgc ttgtttgccc tgattgctgc
     7921 catgctcctg agatttgccg gtacctttcg ttgaagaagc ggttgaagcg ctattcctat
     7981 tgttgttcct attgttttgc gcatcgctgt tgccgtttcc ttttgttgaa gaagtgactg
     8041 cagagctatt gtaatcattc tgcttattat tgtcattatt tttagtggat gatgaattgg
     8101 ttacagtact gctgctggaa ttgttttcat ttttatggct gtgagtgttg tcagcattct
     8161 tatttgattg ttttaccgca ctctctatat cctgagtctc agaatcggaa ttatcagtag
     8221 aatttaatga tctaagcgct atgatttttt tcatgatatc agtaactttc gcgtctttat
     8281 attgatcaac ggtaatggtg gggtctaatg cctggagctt ttgaatcagg tttagttttc
     8341 ccggggtaat acccagtttt ctcgcttcgt ctcttcttga taaagcaacg ttatccttat
     8401 aaacgactgc ggtgtcgccg cttgatttga tcgcatcgtc ggcaccctgc tgggcatttt
     8461 tttcgagcgc cgaagcagca gcggcgttgt cggtttcagc tgttaccgat actattgttg
     8521 aaccgtcgct gttgataaaa ccattttttg atgcggaggc tatcagctct tctaccgcat
     8581 ctttgatatt ggaattgacg acatttttcc ctgccagtat cgtttttcca tcggcgttat
     8641 agcccgttac ggaaacgacc tgattgaaag tgttgacgcc aagttccacg ctgggattga
     8701 tatcgaggct taaataggct accggagttt tataataggc gtagccgcca attgacagac
     8761 cgcatactaa gacagcagca caggctgcaa atacaaattt tttcattgag aacacgctcc
     8821 tcctcttgag aatgatcgtt ttgtcattct tttgcttttc aaggatgtct cttaacgctt
     8881 tttcagtttt ttcaaccagc ttatcttccg catgaatctg gttaagcgca gacttaataa
     8941 ttgtattcat gatcagaacc tccgagtttc tgtcttaaga gttttctcgc tctgtgcaga
     9001 ttcgaataaa tggaattttc tttttgatgc agcagctttg ccatttgggg aacggtgtat
     9061 tcctcataat aaaagagata gataacgtct ttatattttg acggcagaga taaaacagca
     9121 gccattacgt cgttctcttt tttatcctca aacgataatt ccatatcttc gatggagcaa
     9181 atgttttttt tgaaaaagct cttacaaaca tccttgcatt tattaatggt tactctgcac
     9241 aaccacgcct tgatatgttc gtcattttca aatgcaattt cactctgcaa taactttaaa
     9301 aatacttcct gaaatacatc ctcaacgtct gtatgattgc gaagatacat aaaacagatt
     9361 ctgcgaacca tatccgagtg tttttggaga gcttttatta caacatcgtt ggtaccgaac
     9421 gatgcttttt ccatagcttt tttctccctt cacctataat acgactgaat gcgttatatc
     9481 cttgcaaaaa tattttgaaa attattttac atcaacgaat cggatgaacg gtttttgatt
     9541 cttccggaat aataaaaaaa cagccgtaaa aaatacagct gtcctacttc ggctactggc
     9601 agccgttgcc cgcttttagc ccttgcgctt tgcgttgctg cttttcagca ggtttgctct
     9661 ttccttattc ggtttatctg atagtatcag ctacctttta ttgtgtcaat gcagttaagg
     9721 aagggaaatt atgtaagcgg atggcaagac ttcaaattca taagatgata cataatgttc
     9781 gttttcaatc aattacctaa ccagttcaac accggcaaaa ctcaagggcc tgatgcctgc
     9841 tcaacgcaga cctcaagccc ttaagattgc ttcattcaaa ttttgtttaa ctgtttagtg
     9901 tcattttacc aggtctcccc ggtttttcga ggatagagat aaagcctttt agtaacttgt
     9961 ttttctctgt ttgccgatga cgagggagac ggcgacgatg gcgacggcaa acccaagctg
    10021 gatcagcatg caataaaaaa tcggcatgag gtttgcggcg ctgccgacca ccaggctgtt
    10081 gatatcgttg agagctttga tataccaata agtgggggta aagcttgcaa tcgtctgcac
    10141 ggtttttcca agtagcgcct gcggaacgaa aactccgcct ataaagctgc tcccaagcgc
    10201 gaggacgttt gctgcggcgg attgtgcgct ccggctccgg atcagattac cgaccaggaa
    10261 actgatgctc agccacgcaa gcatcagtac gagcgagtta aggcacagca ggatgaaatg
    10321 ccggttcgga agcgattccc gatagatcag aacactcagt cccatgaaca gcgcccaggt
    10381 aacaaatgaa aaggtcaggt tcccgagcag aagctgcata ttgatatgca ccgccttcac
    10441 cggagagcaa agattgcggc ttttaagatc cgattcattg aaaacggtca taatggatga
    10501 gacgccaagg atcaggaccg ccgcaatcac ataggcaaaa taagtgaaat aatagatgaa
    10561 accgcttttt tcagccgggc tgccaaagga ggtgagatgc acgtcggctg ccgttttcag
    10621 gttgcctgag atatcggtcg caagctgtgt ctgggaaacg cccggcaggc tcttctcaaa
    10681 caggctcgcg gtattcagat atcggttgac caaaaaatcc atattcacgc tgcttacgga
    10741 atttgggatg cttgtcttct caagccggat atcatttttc ccgcttaaaa aattttgcgt
    10801 aaaccctttc gggatcctta cgatataatc aacccggttg taaaagaggg catcctgcag
    10861 gtcttgcgtg tcatccgtca gcgggacaat attggcattc cgtgaaaggt agcttttcag
    10921 gctgtttgtc agcggtgacg gatgatcgtt gttgataaag gcaatattcg tctttgtggg
    10981 ggtaaagttc agtgccgaat tgcttcctcc aaaactggaa agcccgattg ccagcgcaat
    11041 gaaaattccg acataaatca tcatgacagg gagattgctt ttaagaatct tgaaataaac
    11101 cttaaagact tgcatatttc tgcctcctta aaacaacaga gacaataaag ataaacaccg
    11161 tcgacatacc gaacagcact ccgatgttcg taaaatatct ggtatgtgta ttataataat
    11221 acagggaata aaaggcgtcg gtaattacgt ttgcagggtt gagccacgcg atcggcggga
    11281 aggcctttat gatggtatac cgggatggag caccatacaa tccggtcaaa aaagacagcg
    11341 ccatggtcag gaaaatcaga agcgcgacct tgatcccttc ccctttttta atgaccgcac
    11401 cgatcagcgc acccatggtc acgcccgtaa aacagccggc gaggcaggcg agaagcacat
    11461 aaccgatctg gttgccgaaa tcaaccttga gcacgaaatt aagataagca atcaggacga
    11521 ctgccgacgt aaattcaact gctgtggctg cgcagagggc cgaactgaaa attttcagct
    11581 tgtgaacggg tgccacactg acccgtgcgc cctgtgcgga aaggtcggcc tgcaccgcta
    11641 cgacttcttt tatcccccag aatcctccat aaagacaggc cattgcaatc agcgaataga
    11701 actcgttgag gatgtcgttg ggtgccgctt ttgtaggcga gacttctttg agataatcca
    11761 tttgggatag atttgcggta aaattttgta tggcggccgg atttttgctg aggatggtct
    11821 ttgctgccgc gctcgtttgc acataatcat ccgcaaattc cttcagaata gtctggttga
    11881 tgccggattc ctttacgaac acttcaattt ttccgctgcc ggaaagttgg aaataaccgt
    11941 cgattttgct gtttttcaac agatctacgg cacgctgttt tgcggcgggg gtaacgacga
    12001 acagacggct ctggcccggt ttcacatcgg tcgatactgc tgcgagcgcc gagcggaagg
    12061 ccgaattgtt ctgatagtta gaatcgttta cgaccgcgat tttgatgttt tcaaaattct
    12121 gagagctttg cagattggaa aacgccatat ggaaaaatgt cgcgaggacg atgggaaaca
    12181 ataaggtcca gaacaacagc tgccgatctc tcaccaggca tttgaaacga ttgacgaaaa
    12241 catgtagaaa catcacttcc tgccccctaa tccctgagct ctttgccggt aatttcaagg
    12301 aaaacatcgt tgagcgttgg cagctcgcaa taaattttac cgtaggcgat ctctttcgct
    12361 ttgagatatt ccagcacatc ggtgaggttg tttttgcccc tgttcgactt gatcacaacc
    12421 ctgttttcac tctcgttgta atcaaccgaa ctgatgttgg gcaggctcct gagatcctcc
    12481 agcagggaat tatgaaagtt gaagatctcc gcgatgattt tttctccggt gttgatcatt
    12541 cctttgagct catcgtttgt gccgctggcg atgattttgc ctttatccat aattgcgatc
    12601 cggctgcaga tctgctcgac ttcttccatg tagtgagagg tgtagatcac agtggcgccc
    12661 tgccgattca gtttcagaat gccctcgagg atgttgttac ggctttgagg gtcgaccgct
    12721 accgtcggct catcgagtat aatcagtttc ggtttgtgcg caatgccgca ggcgatgttc
    12781 agcctgcgaa gcagcccgcc gctcagcttt ttgggcacaa attttctgta ttcgtccagt
    12841 cccacaaatg aaatcgcctc gtctaccagc tcggcgcgct ttgccttatc cgtaatataa
    12901 agcccgcaga aataattgat attttcatag accgtcagtt cgctgaaaac cgccacattt
    12961 tgcatgatga tgccaatatc gcgcttgatg gcataagaat tcggcgacat aggccggccg
    13021 aatacctcaa tgctcccttt atcgtatttg agcaatgaca gaatgcagtt gatggccgtt
    13081 gtctttcccg agccgttcgg ccccaaaagc ccaaaaactt ctccttcatc aacatccagg
    13141 ctgaaatgat ccagcgcaat taaatcgccg taccgtttca ccagattttc taccttaaca
    13201 accatatcat ttcctccggt tcaatttata aaaattcaat tcgactctca tacctgtatt
    13261 ttaccttttt tctccgtact ccgatagtga atttcttccc gatacgg
//
LOCUS       DATALY010000200         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_129907,
            whole genome shotgun sequence.
ACCESSION   DATALY010000200 DATALY010000000
VERSION     DATALY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129907"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VHR42_05845"
     CDS             <1..472
                     /locus_tag="VHR42_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3026733.1"
                     /translation="DDIFTAIRIAKQFDLDYVIVHCTEGHLISEELCNENVRALSGPF
                     LCDRSKPELSQMTPKSPGIMSKAGIPVALITDHSVVPIQYLMLCAAIAVKEGMDRREA
                     LRAVTINPARILGLENRIGSLKAGKDADLAIYSGDPFSVFTQVKCVIINGKRVR"
     gene            474..1316
                     /locus_tag="VHR42_05850"
     CDS             474..1316
                     /locus_tag="VHR42_05850"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX3026734.1"
                     /translation="MREIHTDVIVETVKKLCVEANLKLPGDVCDALKRAREEEQSEVA
                     RGVIGDLEQNLVAAENLGVPVCQDTGMAVIFLELGQEVHVTGGDLYQAIQKGVGRGYI
                     EGRLRLSVVGDPINRVNTGDNTPAVIHTQITAGDRLRITVAPKGFGSENMSAIRMFTP
                     SATRGDIVSFVTDTVLKAGSKPCPPVIVGVGIGGDFEYSAILAKRALLRNLNQRNSSD
                     YYRQLEQDMLDQINRLGIGPQGFGGTVTALAVNIETFATHIAGLPVAVNMGCHVTRHR
                     SAVL"
     gene            1478..1999
                     /gene="raiA"
                     /locus_tag="VHR42_05855"
     CDS             1478..1999
                     /gene="raiA"
                     /locus_tag="VHR42_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX3026735.1"
                     /translation="MKITITGRKVTLKDRFKERVEKKLEKFERFFDEETEAFVTVTLE
                     KERQTVEITIRYKGMIYRAEETTGDMLYSFDNAVDALMRQIRKNKARLEKRLKEGAFE
                     EAPEDSDKKSKFNIVRVKKFAVKPMDFEEAVLQMNLLGHEFFAFLNEETYQVNVVYKR
                     HDGSYGVLEPVAG"
     gene            2286..>2914
                     /locus_tag="VHR42_05860"
     CDS             2286..>2914
                     /locus_tag="VHR42_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX3026736.1"
                     /translation="MNQIKIACPCLFGLESLVAEEIRRLGYEQTVSTNGRVVFEGDTL
                     AIARANLNLRCAERVLVLLAEFEARSFEELFQGVKRLPWEDWIGRDDVFPVKGWSLNS
                     ALHSVPDCQSIIKKAVVEKLKTVYHVDWFQETGAKIQIQFSILKNIVSVYLDTSGEGL
                     HKRGYRQNANEAPLKETLAAAMVKLSHFYTDRPFYDPFCGSGTILIEAA"
BASE COUNT          789 a          606 c          790 g          729 t
ORIGIN      
        1 ggatgatatt tttaccgcta tccggattgc caaacagttt gatctggact atgtgattgt
       61 tcactgcacg gagggacatc tgatttccga agaactctgc aatgagaatg ttcgggcgct
      121 ttccgggcct tttttatgcg acaggagcaa accggagctt tcccaaatga ccccgaaaag
      181 ccccggaatt atgagtaaag ccggtattcc ggtcgccctg atcaccgatc attcggttgt
      241 gcccatacaa tatttaatgc tctgcgcggc catcgccgta aaagaaggga tggaccgccg
      301 tgaggctcta cgggcggtga ccattaatcc tgccagaatt ctcggcctcg aaaacaggat
      361 cggttcgctc aaagcgggca aagacgccga tctcgcgatt tacagcggag accctttttc
      421 agttttcacc caggtgaaat gtgtgatcat caatggaaag cgggtaaggt agcatgaggg
      481 agatacatac cgacgtaatt gtggaaacag taaaaaagct ctgtgtggaa gccaatttaa
      541 agcttccggg cgacgtttgc gacgctctga aaagagccag ggaagaggaa cagtcggagg
      601 ttgccagggg agtcatcggc gatttggaac aaaacctggt tgctgcggaa aatctgggcg
      661 tgccggtttg ccaggataca ggcatggctg tgatttttct cgaactcggt caggaggttc
      721 atgttaccgg gggcgatctg tatcaggcca tacagaaagg agtcggccgc ggttatatcg
      781 agggacgttt acggttatct gtggtgggtg atccgatcaa tcgggttaac acgggagata
      841 acacgccggc ggtcatccat acgcagatca ccgcggggga ccggctgcgc attacggttg
      901 cgccgaaagg attcggcagc gagaacatga gcgctattcg aatgtttacc ccttccgcaa
      961 caagagggga catcgtctcg tttgtgacgg atacggtgtt aaaggccggc agtaaaccct
     1021 gccctcccgt catcgtgggg gttggaatcg gcggcgattt tgaatattcg gctattctgg
     1081 caaaaagagc gctgctcaga aatctgaatc agcgcaactc ttcggattat taccgtcaac
     1141 tcgaacagga tatgctcgat caaatcaacc ggctgggtat cggcccgcag ggcttcggcg
     1201 gaacggttac cgctcttgcg gtcaatattg aaacctttgc aacccatatc gcggggcttc
     1261 ctgttgccgt taatatgggc tgtcacgtta caagacaccg gtccgcggtt ttataaaaaa
     1321 cacagaaatc atgcctgttt gtcaaaaaac cgcaaatagt tacttcgtat ttcataaatc
     1381 gacttggatt attgataaaa tgtgatataa tatatccata cagtgaatat tttatgtgta
     1441 atttatccaa gataattgca caaaaggagc tgactttatg aaaatcacga ttaccggcag
     1501 aaaggtgacc cttaaagacc gttttaaaga aagggtagaa aagaagctgg aaaaattcga
     1561 aaggtttttt gacgaagaaa ctgaggcttt cgtaacggtt acactggaaa aggaaagaca
     1621 aacggttgaa ataaccattc gatataaggg aatgatttac cgggcggagg agacgaccgg
     1681 cgacatgctc tactcgtttg acaacgcggt cgacgcgttg atgcggcaga ttcgaaagaa
     1741 caaagcgcgg ctggaaaaaa gactgaaaga aggagctttt gaagaggccc cggaagacag
     1801 cgataaaaag tcgaagttca atattgtcag agtgaagaaa ttcgccgtaa agccaatgga
     1861 ctttgaggaa gccgtgctgc agatgaatct gctgggacat gagttctttg cctttctcaa
     1921 tgaagaaacc tatcaggtta acgtggttta caaacgtcac gacggaagtt acggcgttct
     1981 tgagccggtg gctggttaaa ccggcagggg ccgtgcgatt ataaaatgaa tcaagccaaa
     2041 tatttcgagg ccgcattttg tgcggccttt ttcttttttg tgtaaaatcc gcttggcttt
     2101 tgaaagaatt tcaaaagagg aaaattttat tatacgggca tttgaccggc cgatttatga
     2161 tataataaaa atattatgga tataacagtt cccgatgcgg ttttctgccg tcctgacgga
     2221 gatgttcaaa aaatctttct gcgctgcctt gggctccgcg gaaattatca gacggaaggt
     2281 taacaatgaa tcagataaaa attgcatgcc cctgtctttt tggcttggaa agccttgttg
     2341 ccgaagaaat caggcgattg ggctatgaac agactgtctc gaccaacggc agggttgttt
     2401 ttgagggcga tacgctggcc attgccagag ctaatctgaa tttgcgctgt gcggaacgtg
     2461 tgctggtact gcttgcggag tttgaggcgc gaagctttga agaactgttt cagggcgtga
     2521 aaaggctgcc gtgggaagac tggatcggca gagacgatgt gtttcccgtt aagggctggt
     2581 cgctcaattc tgcgctgcat agtgtgcctg attgccagtc gatcattaaa aaagccgtag
     2641 tggagaaatt gaaaacggtt tatcatgtcg actggtttca ggagacgggg gcgaaaatac
     2701 aaattcagtt ttccatttta aaaaatatcg tttcggttta tctggacact tccggtgaag
     2761 ggctgcataa aagaggctac cggcaaaatg ccaatgaggc tccgctgaaa gagactctgg
     2821 cggcggcgat ggtgaagctt tctcattttt atacggacag gcctttttat gatccgttct
     2881 gcggttccgg aacaatttta attgaagcgg ccat
//
LOCUS       DATALY010000201         5180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_388408,
            whole genome shotgun sequence.
ACCESSION   DATALY010000201 DATALY010000000
VERSION     DATALY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388408"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VHR42_05865"
     CDS             complement(<1..240)
                     /locus_tag="VHR42_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX3026737.1"
                     /translation="MGDSNLKLFSSKGHAGAIDFFKYIGPGLLVTVGFIDPGNWASNI
                     AAGSEYGYVLLWMVTLSTVMLIVLQHNAAHLGIVTG"
     gene            613..1125
                     /locus_tag="VHR42_05870"
     CDS             613..1125
                     /locus_tag="VHR42_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX3026738.1"
                     /translation="MLNQELASLLNAQVNMEWYSAYFYLDIYSYYTDKNLDGFGNWFY
                     VQTQEERDHAMLFIKYLLNNDEKVLLQTIQAPDKIFEDFREPANAAFEHELKVTASIN
                     HIYAVAYEQKDFRTMQFLDWFVKEQGEEEKSTQDIVKRYDLFGKDPKGLYLLDSELAA
                     RVYAPPSLVI"
     gene            complement(1252..1341)
                     /locus_tag="VHR42_05875"
     tRNA            complement(1252..1341)
                     /locus_tag="VHR42_05875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1306..1308),aa:Ser,seq:gct)
     gene            complement(1374..1462)
                     /locus_tag="VHR42_05880"
     tRNA            complement(1374..1462)
                     /locus_tag="VHR42_05880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1426..1428),aa:Ser,seq:tga)
     gene            complement(1729..2121)
                     /locus_tag="VHR42_05885"
     CDS             complement(1729..2121)
                     /locus_tag="VHR42_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026739.1"
                     /translation="MEKKVTLFVTGGVIYPSLEILCRGRSDFSMAVAGGLCLCLIDRV
                     CNCKLGSCTLFTRCFAGSGIITSVEFAIGYLVNIVMKLNVWDYSQMPLNLMGQICLPF
                     SLLWFFLTIPAMQLCLLYDRVRKAVKSA"
     gene            2351..4657
                     /locus_tag="VHR42_05890"
     CDS             2351..4657
                     /locus_tag="VHR42_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3026740.1"
                     /translation="MQHKNKLVLAAGVVTALLFAAGALLLINLHSIAVKAVQTSLSDF
                     SEQAVQAVRTEIQGNLNVLKALSGLDAFSDPDDFSNRKRILNQEASQNTFRLVVYSDP
                     SGKAVTNHKLTLQLGNRAYFQKALAGQATISYDFIDADNSRKEIIYCVPVIRANRVCG
                     VLAAAAQDSSRFSGLESLNAERDITVWVLRKDGTVIDGKQDRAGSGDFFSASGVSADA
                     SLTNRLKSDFLNGVSGTSVLRLNGSVRQTIYMPIPGSNGWILWAEKNDSGLFENDQSL
                     LFTAVLFLLLLFVLAIVLFYFFRFQRISHEASIRSDEQKRYFVYADSLTNLPNRKGMK
                     RLFTPWSADCKKCSQNGGAFFLDVDNFRSVNNTFGHDSGDKFLCETATRLRRILGSGA
                     QIGRIGGDEFMVLVSGLAAVDDLELYAGKILTVFKEPYLLNGIVIQLSCSIGAILYKA
                     RDEDCGSEFDDILNRGEFLMQEAKAANKGSYILFNDAFGDRIDHQLQLEAALKSSIAS
                     SELLCYFQPQYNCQEKTLVGFETLARWKSARFGMVSPSEFIPMAEKSGFINDLGRFMI
                     EKTFAFAKSVEGRGLTISFNASPVELLQANFTDYIIGRFDYYDLAPNSVALEITESCL
                     IESFDEVIRKLEELRAHGIPIYLDDFGTGFSSLTYLKNLPINAVKIDKSFIDEIVTNN
                     VEKDIVGMIIRLAHRLDLLVIAEGVETQEQVDCVAECGCNIVQGYIISRPVPQNEVLS
                     LLDLLDKPFGAEADFVQKQTVQKLPGNL"
     gene            complement(4730..>5180)
                     /locus_tag="VHR42_05895"
     CDS             complement(4730..>5180)
                     /locus_tag="VHR42_05895"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEX3026741.1"
                     /translation="KTPAAEKLADLLLQKWNAQDPKHPLGKANYDAISGGFRKKLCFI
                     TTAVCSSLHKGDSCEELNTFRRFRDGWLAATEQGPAKIQEYYLFAPMIVEAIDRSEKS
                     GEEYRRIWQTYLEPCFRELRGGRNEACSERYEGMMAELEQKWFGILQ"
BASE COUNT         1372 a         1293 c         1210 g         1305 t
ORIGIN      
        1 tccggttaca ataccgagat gggccgcatt gtgctgcaaa acgatcagca taaccgtcga
       61 cagcgtgacc atccacaaaa gcacatagcc atattccgaa ccggctgcaa tgttggaggc
      121 ccagttgccg ggatcgataa accctaccgt aacaagcagg cccggcccta tgtatttaaa
      181 aaagtcgatt gctccggcat gtcctttgct ggaaaataac ttcaaatttg aatcacccat
      241 tccctcttcg atttcgccgt tgatatgctg caccatcacc tttgtcggcg cttcactttc
      301 cgtactatag caatatctta ccacaaaaac cgtcagcgga aatcaaaagc ctgtaaaaat
      361 cagggccccc gacaattctg aaatacggca gttatggaac ttgacgaaaa tgaaaagcaa
      421 aacagctgca atcccggcaa aaattagcat tgattaaagt ggataatatt cgtatacttt
      481 atttgtaaaa aaaagcaaca tggttttgta taaagatctg tgctccgctg atccgggcac
      541 gaaaccggtt atcgccggta tgaatcccgg aactcagtga atactagatt cttagaaaaa
      601 ggagaggaaa acatgctgaa tcaagagtta gcttctttat taaatgctca ggtcaatatg
      661 gaatggtatt ccgcttattt ttatctggat atttacagtt actatacgga caaaaatctc
      721 gacggatttg gaaattggtt ttatgttcaa acgcaagaag agcgcgacca tgcaatgctg
      781 tttatcaaat atctattaaa caatgatgaa aaggttcttc tgcaaaccat acaggcgcca
      841 gataaaattt ttgaagattt tcgtgaaccg gccaatgcgg cctttgagca cgaattaaaa
      901 gtaacggcat ccatcaacca tatttatgcg gttgcttatg agcaaaaaga ttttcgcacc
      961 atgcagtttc ttgattggtt tgtaaaagag caaggcgaag aagaaaagag cacgcaggat
     1021 attgtcaaga gatacgatct tttcggaaag gaccccaaag ggctttattt gcttgattcg
     1081 gagcttgccg caagagtcta tgcgccccct tctctggtaa tttaaaaagc cttgaatgta
     1141 gagatgacat aataaaccgg accaccgcga tcagcacagt aaacgtaggg gtaattcttt
     1201 caaaacagat atgaaaaagc ctgtaatctt aaaagattac aggctttttc gtggcggaga
     1261 aagagggatt tgaaccctcg cgccggtttc ccgacctact cccttagcag gggagcccct
     1321 tcaccacttg ggtatttctc caggatggtg aatcaacaaa ttttatctaa aaatggcgga
     1381 gaggaagaga ttcgaactct tggtcccttg cgggatcact agttttcaag actagctcca
     1441 taaaccactc ggacacctct ccggattcgt ttcctcacag gtgcgagatt aataatacca
     1501 tatttccact taccctgtca atggaaatcc cccaaaaaat caattttgcg cggtttttaa
     1561 gcagatctca cggtcttatg taaaatgggc tgcctctccc aaagaggccg cgcataaaaa
     1621 atgtgtcctg atcgggctgc aaaggccttt cccagtcaca ttttaaggaa ttctacaatt
     1681 ttcggcgctt cgttataacc acccatttac ttttgacaca gtttctcctt aggcgctttt
     1741 cactgctttc cgtacccggt catataatag gcagagctgc atggcgggaa tcgtgagaaa
     1801 aaaccaaagc agggaaaacg gaaggcagat ctgccccatt aaattcaacg gcatctgcga
     1861 ataatcccac acattcagct tcatgacaat attgacaagg tatccgattg caaactcgac
     1921 cgacgtgata atgccggaac ccgcaaagca cctcgtaaac aacgtgcatg atccaagctt
     1981 gcagttgcag acacggtcaa tcaggcatag gcaaagcccg cccgcgaccg ccatggaaaa
     2041 atcacttctt ccccgacata aaatttccag agaaggataa atcactccgc ccgtaacaaa
     2101 aagcgtaacc tttttttcca tgcgaatccc tccttatcga tggattcatt atttgcatga
     2161 gcacacaatt tattcagcag cgcgaaagga tgatattggt aaaacatttc ggatatcttt
     2221 cgaaaaaatt tcggttgttt ttgccaaaaa aagctccgat acttttcctc caaatgtctg
     2281 ttggaataat gaaatttatt tttcttccct gtataatatc tcttaggaat aaatgaatcc
     2341 aggtgatgaa gtgcaacata agaataagct ggtccttgca gccggggtag tgaccgctct
     2401 gctttttgca gccggcgccc tgttgcttat aaaccttcac agcatagccg taaaggcggt
     2461 acagacctcg ctttcggatt tctcagaaca ggccgtgcag gccgtgcgca cggaaattca
     2521 gggcaatctg aatgtattaa aggcactctc cggtctggat gcattctccg atcccgatga
     2581 tttctccaat cggaaacgta ttctcaatca ggaagcatcc cagaacacat ttcgtctggt
     2641 cgtatattcc gacccctccg gtaaggccgt taccaatcat aaacttacgc tgcagcttgg
     2701 aaaccgcgcc tattttcaaa aggcattggc cgggcaagca accatctcct atgattttat
     2761 tgatgccgac aacagccgga aagaaattat ttactgcgtt cccgtcatcc gcgcaaatcg
     2821 ggtgtgcggc gtccttgcgg ctgccgcgca ggacagctcc agattcagcg gtctggagag
     2881 tctcaacgca gagcgcgata tcactgtgtg ggttttgcgg aaggacggaa ccgtcatcga
     2941 cgggaaacag gatcgagccg ggagcggtga ctttttttct gcctccggtg tttcggcgga
     3001 cgcgtctctg acaaacaggc tgaaatcgga ctttttgaac ggagtttccg gaacgtcggt
     3061 gctccggctc aacggctccg tacgtcagac catctatatg ccgattccgg gttccaacgg
     3121 atggattctt tgggcggaaa aaaacgacag cggtctgttt gaaaacgatc agtccctgct
     3181 gtttacggct gttctttttc tgctgctgct gtttgttctg gcaattgtac tgttctattt
     3241 cttccggttt caaagaatca gtcatgaagc gtcgatccgc agtgatgagc aaaagcgcta
     3301 ttttgtctat gcggattcgc tcaccaacct tcccaaccgg aaaggaatga agcggctgtt
     3361 cacgccgtgg tctgcagact gcaaaaaatg ctcgcaaaac ggcggcgcct tttttctgga
     3421 tgtggacaac tttcgttccg taaacaacac attcgggcac gactccgggg acaaatttct
     3481 ttgtgaaaca gcgacgaggc ttcggcgcat tctgggctcc ggtgcgcaga tcgggcggat
     3541 cggcggcgac gagttcatgg ttctggtcag cgggctcgcc gccgtggatg atcttgaact
     3601 gtatgcgggc aaaattctga ccgtattcaa agagccatat ctgctcaacg gaatcgtcat
     3661 tcagctatcc tgcagcatcg gagccattct ttacaaagcg cgcgacgaag attgcggcag
     3721 cgaattcgac gacattctca accgcggcga atttttaatg caggaagcaa aggccgccaa
     3781 caaaggcagc tatattctgt ttaacgacgc gttcggcgac cgaatcgacc atcagcttca
     3841 actggaagcg gcgctgaaat cttccatcgc aagcagtgaa ctgctttgct actttcagcc
     3901 gcaatacaat tgccaggaaa aaaccctcgt cggttttgaa acgcttgcgc gctggaaaag
     3961 cgcgcggttc gggatggttt ctccctcgga gttcattccc atggcggaaa agtccggctt
     4021 tatcaacgat ctgggccgat ttatgattga aaagaccttc gcctttgcga aaagtgtgga
     4081 gggccgcggc ctgacgattt cgtttaacgc ttcgcccgtc gaactgctgc aggcaaactt
     4141 taccgattat atcatcggcc ggtttgatta ttatgatctc gctccgaaca gcgtcgcgct
     4201 ggaaattacg gaatcctgcc tgattgaatc gtttgatgag gtgattcgga agcttgagga
     4261 actgcgggca cacggaattc ccatctatct tgacgacttc ggcactggtt tttcctctct
     4321 gacgtatctt aaaaatcttc ccatcaatgc ggtgaaaatc gacaagagct ttattgacga
     4381 gatcgttacg aacaatgtgg aaaaagatat tgtcggcatg attatccggc ttgctcaccg
     4441 gctggacctt ctggtcatcg cggaaggcgt tgaaacgcag gagcaggtcg actgcgtggc
     4501 cgaatgcggc tgcaatatcg ttcagggcta catcatcagc aggccagttc ctcagaacga
     4561 ggtgctgtct ctgctggacc tactagacaa accgttcggt gcggaagcgg acttcgtcca
     4621 gaaacaaacc gtgcagaagc tgcccggaaa cctttaacac aaccgaacgc aaaagcaacg
     4681 gggccagtga actacatcac tgaccccgct gccgtatttt taaatgaact tactgcagaa
     4741 tgccgaacca cttctgctcc agttccgcca tcatgccttc ataccgttcc gagcaggcct
     4801 cgttccgccc gccgcgcagc tcgcggaagc atggctccag atacgtctgc cagatacgcc
     4861 ggtactcttc gccgcttttt tcagagcggt cgattgcctc tacgatcatg ggggcaaaca
     4921 gatagtattc ttgaattttc gccggtccct gttccgttgc ggcaagccag ccgtcgcgga
     4981 aacggcggaa tgtattcagt tcctcacagg aatccccttt gtgcagcgaa gaacagaccg
     5041 cagttgtaat aaaacaaagc tttttccgga atcccccgga aatggcatcg taattcgcct
     5101 tgccgagcgg atgcttcggg tcctgtgcgt tccatttctg cagcagcagg tccgcaagct
     5161 tctccgcggc cggagttttc
//
LOCUS       DATALY010000202         4381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_304876,
            whole genome shotgun sequence.
ACCESSION   DATALY010000202 DATALY010000000
VERSION     DATALY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304876"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VHR42_05900"
     CDS             complement(<1..665)
                     /locus_tag="VHR42_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX3026742.1"
                     /translation="MARKFKTMDGNSAAAHVSYAFTDVAAIYPITPSSVMAEETDKWA
                     AGGQLNLFGRPVKLAEMQSEAGAAGAVHGSLAAGALTTTYTASQGLLLMIPNMYKIAG
                     ELLPNVIHVSARALASHALCIFGDHSDVYACRQTGYAMLCSSSVQEVMDLGAVAHLAT
                     IKGRVPFIHFFDGFRTSHEIQKIQVWDYKDLADMLDWDAVDRFRKNALNPEHPVQRGT
                     AQNP"
     gene            1172..1294
                     /locus_tag="VHR42_05905"
     CDS             1172..1294
                     /locus_tag="VHR42_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX3026743.1"
                     /translation="MLQLLLLIVLILFNAFFAASEIAIITLNDNKIRKNGRGRT"
     gene            1275..2540
                     /locus_tag="VHR42_05910"
     CDS             1275..2540
                     /locus_tag="VHR42_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX3026744.1"
                     /translation="MAEDGHKKAQQVLKLTSDSSNFLATIQIGVTLAEFLTSATAAQS
                     LASPLANWFIRHFSSLMPYISIVQTASVIVVTLVMSYFSLVFGELVPKRIAMQKAEPL
                     SFAFIGALRGVAAITKPFVRLLSASTNLVVRLFGMDPHANEENVTEEEIRMMVDVGGE
                     NGVIEDTQKEMINNIFEFDDITAEDIMTPRTEVESVEVSENIAEALRICVDGGYSRIP
                     VYEEDIDNIVGVLYVKDLLPYVGQTIPKDVSLRLLMRKTYFVPGTKRCGDLFAAMTEK
                     HVQMTVVVDEYGGFAGIVTMEDLLESIVGNIQDEYDHEEEEVQRTSDNTFEVDGSVNI
                     EDLGELLDVKFPEGDYETIAGYIMDVLGRIPGEDEHPSVQLDNVTFTVMKMDERRIER
                     VHIEVKPKEKEETEDEPHDEKEKGSTDKE"
     gene            complement(2594..3136)
                     /locus_tag="VHR42_05915"
     CDS             complement(2594..3136)
                     /locus_tag="VHR42_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026745.1"
                     /translation="MKKTGHLFIIGIIIIAILVLAVFTGYKSMKPSVSEKDIAVEAVL
                     GSNNTGVTNDKEYVLDISMTKNKDCHIIVYPYIEGLGGMAFNNVDDKVYYVPNNIGYD
                     TLTEPVAVDELKKEGAVETGKDISLDGFWFPYNAGQYKTRVYLDGTDSFKENPVLVCV
                     YTEKKLGKQFTWAKTVPINN"
     gene            complement(3258..3701)
                     /locus_tag="VHR42_05920"
     CDS             complement(3258..3701)
                     /locus_tag="VHR42_05920"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3026746.1"
                     /translation="MELFQIRVTGILLRGDAILLVQQSISKNRGWSLPGGRAEAHELL
                     SEALIREMREETGLTVKVKKLLYVCDKPDSSPPLLHITFLLGQAGGEIALPTNEHDEN
                     PISDVRFVPVADLESYGFTRRFQELVSNGFPDAGNYKGLKCNIGL"
     gene            3834..4355
                     /locus_tag="VHR42_05925"
     CDS             3834..4355
                     /locus_tag="VHR42_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3026747.1"
                     /translation="MCGIVKTYKQPVGATRFIGIKYGDEDRDGGGFGGQWKEWFAAGR
                     FDKLESLLTGEFKRSYEDSSAYIGLMRWKDGEKFEYWIGMFLPEGTEAPEGYEFVDFP
                     ASNFGVCWLRGPESEIYGKEDKCAERLSQDGYEIAADEKGAWWFFERYGCPRFTTPDE
                     NGNIILDICHFVK"
BASE COUNT         1203 a         1025 c         1027 g         1126 t
ORIGIN      
        1 gggttctgcg cggttccgcg ctgtacagga tgctcagggt tcagcgcatt cttgcggaag
       61 cggtcgacag catcccagtc gagcatatcg gcaaggtctt tataatccca aacctgaatt
      121 ttctgaattt catgagaggt acggaatcca tcaaagaaat gaatgaacgg tacgcggccc
      181 ttaatggtgg caagatgggc aacggcaccg agatccatga cttcctggac actggaggag
      241 caaagcattg cataacccgt ttggcggcag gcgtacacat cggagtgatc accgaaaatg
      301 cacagtgcgt gagaagcaag cgcacgagcc gagacatgga ttacgttggg cagcaattca
      361 cccgctattt tatacatatt cgggatcatc aacagtaagc cctgtgatgc cgtataagta
      421 gtcgttaaag caccggcagc gagcgaaccg tgaacagcgc cggcagcacc agcttcagac
      481 tgcatttctg ccaactttac gggacgtcca aacaggttta actgcccacc cgcagcccac
      541 ttgtcggttt cttccgccat gacagaggac ggggtgatgg ggtagatggc ggcaacgtcg
      601 gtaaatgcat acgatacgtg tgcggcggcg ctgttaccat ccatggtttt gaattttctt
      661 gccattcaat acttcctcct ttaaggatta aaaatgtgag tgaaatcccc caaaagggac
      721 gataaaccac aaagaaaaaa caacaaaagt gccttatttg tgtactgcca tggggaattt
      781 tatcaaatac aaagccatct gtaatagtaa cactttcgaa agaaactgta aactataaaa
      841 gtacggattt tcgttataat tttgtgaatt ttttagcaga aggttactta aagccttaaa
      901 aaggtgaaat tttaatgcct tgcgcggatt atcgcgatga catggaaaag ccgctctgta
      961 gcggggctta tcggtgcttt tccccgccgc gtatatcggg tgtccggttg acataactcc
     1021 cgaaaacaga tattattgaa ttaatgttat ttttacctat ttcattaagt ttataaaatg
     1081 gtacatacta aaagagcagc ggcttttgcc gcacatacga aaggggaaac tttctcacat
     1141 ggattcaggc cctgcgcccg caggtaattt tttgctgcag cttttgctgc tgatcgtttt
     1201 aattctattt aatgcgtttt ttgccgcatc ggaaattgcg atcatcacac tgaacgataa
     1261 caaaatacga aaaaatggca gaggacggac ataaaaaagc acagcaggtt ttaaagctga
     1321 cgtccgattc ctctaatttt ctggctacga tacagatcgg cgttacgctt gcggaatttc
     1381 tgacttccgc gacagcggcg cagtctctcg ccagcccgct cgcgaactgg tttatccgcc
     1441 atttttcgtc attgatgccc tatatcagca tcgttcaaac cgccagcgta atcgttgtga
     1501 cgctcgttat gtcctatttc tcgctggtat tcggggaact cgtcccgaaa cgcattgcga
     1561 tgcagaaagc agaaccgctg tcttttgcat tcatcggtgc gctgcgcggt gtcgccgcca
     1621 taacaaaacc ttttgtccgc ctgctgtccg cctcgacgaa tcttgtcgtg cgccttttcg
     1681 gaatggatcc gcatgcgaat gaggaaaacg tcaccgaaga agaaatccgc atgatggtgg
     1741 atgtcggcgg tgaaaacggc gttatcgaag acacccagaa agaaatgatc aacaacatct
     1801 tcgaatttga cgatattaca gccgaagata tcatgacgcc gcgtaccgag gtggaatcgg
     1861 tcgaggtcag cgaaaacatt gcggaagcgc tgcgcatctg cgtggacggc ggttattccc
     1921 gcatcccggt ttatgaagag gatatcgaca acattgtcgg cgtgctatat gtaaaagacc
     1981 tgctgcctta cgtcggccag acgattccga aagacgtgtc cctgcgcctt ctgatgcgga
     2041 agacctactt tgtgcccgga acaaaacgct gcggagattt gtttgccgcg atgaccgaga
     2101 agcacgtcca aatgaccgtc gtcgtcgacg aatacggcgg atttgccggt atcgtgacca
     2161 tggaggatct gctcgaatcg atcgtcggca acatccagga tgaatacgac cacgaggaag
     2221 aagaagttca aagaacaagc gacaacacgt ttgaagtgga cggctcggtc aatatcgaag
     2281 atctcggcga actcctggat gtgaaatttc cggaagggga ttatgaaacg atcgccggat
     2341 atatcatgga tgttctcggc cgcatccccg gcgaagacga gcacccgtcc gttcagcttg
     2401 ataacgtgac ttttactgtg atgaaaatgg acgaacgccg tatcgagcgc gtgcatatcg
     2461 aagtgaagcc gaaagaaaaa gaagaaaccg aagacgaacc gcatgacgaa aaagagaaag
     2521 gttcgaccga taaagaataa taaaaacagg cagttcgcaa tgaactgcct gttttactat
     2581 ttcaccggct ttattaatta tttattggca ctgtctttgc ccatgtgaat tgcttcccca
     2641 gtttcttttc cgtatatacg caaactaaaa ccggattttc tttgaaactg tccgttccgt
     2701 ccaaataaac tcttgttttg tattgtcctg cattataggg aaaccagaag ccgtctaagg
     2761 aaatatcttt tccggtctca acagcaccct ctttttttaa ttcatcaacg gcaaccggtt
     2821 ccgtcaaggt atcgtatccg atattgttag ggacataata cactttatca tcgacattgt
     2881 taaaagccat tccccccagt ccttcgatat aaggataaac aataatatga caatccttat
     2941 tttttgtcat actaatatct aaaacgtatt ccttatcgtt agtaactcct gtattattag
     3001 aacccaaaac agcttctacg gcaatgtcct tttcactgac tgacggtttc atacttttat
     3061 accctgtaaa tactgctaaa acaagtatgg ctataatgat gatcccgatg ataaataggt
     3121 gcccagtttt tttcatatag ccgccccctt aatggatcat tgcttactta ccaattatac
     3181 aatggtttcc attaattaac aagtgttgtt attcatatcg cagataatga agggggtatg
     3241 ccgcttatta aaccttgcta taatccgata ttacatttca gccccttata atttcccgcg
     3301 tccggaaacc cgtttgaaac caactcctga aaccttcttg taaacccgta ggattccaaa
     3361 tcggcgacag ggacaaaccg gacgtcggat atcgggtttt cgtcgtgctc gttcgtgggc
     3421 agcgcaatct ctccgcccgc ctgcccgagc agaaacgtaa tatgtaaaag cggagggctg
     3481 ctgtccggct tgtcgcagac ataaagcagt tttttcacct taaccgtcag acccgtttcc
     3541 tcccgcatct cccggataag ggcttcgctt aaaagttcat gcgcctctgc cctgccgccc
     3601 ggcagcgacc atcccctgtt tttcgagatc gattgttgaa cgagcagaat tgcgtcgccc
     3661 cgcagcagta tcccggtaac gcgtatttga aaaagctcca cttccgtaac cccctatatt
     3721 acattaacat ccctgttgat caagcaggat tttaataagt aaattatata ccaattcgct
     3781 tccatatgat ataataagac aaatttggat tttaatataa ggaggcctgg aaaatgtgcg
     3841 ggattgtcaa aacatataaa cagccggtcg gtgcgacgcg tttcatcgga atcaaatacg
     3901 gtgatgaaga ccgagacggc ggaggattcg gtggacaatg gaaagagtgg ttcgcagccg
     3961 gtcgtttcga taaactggaa tccttactga ccggagagtt taagcgttct tatgaagatt
     4021 ccagcgccta tatcggactg atgcgctgga aagacggcga aaagttcgaa tactggatcg
     4081 gaatgttcct cccggaggga acagaggctc ccgaagggta tgaattcgtc gattttccgg
     4141 catcgaattt cggcgtctgc tggctgcgcg gaccggaatc ggagatatac ggcaaggagg
     4201 ataaatgcgc cgaaagactg tcacaagatg gatatgaaat cgccgcggac gaaaaaggcg
     4261 cgtggtggtt tttcgagcgc tacggatgcc cgcgttttac aacgcctgat gaaaacggaa
     4321 atattatttt agatatctgc cattttgtaa aatagactcg cacctgaaag gatgatatcc
     4381 a
//
LOCUS       DATALY010000203         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_218879,
            whole genome shotgun sequence.
ACCESSION   DATALY010000203 DATALY010000000
VERSION     DATALY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218879"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VHR42_05930"
     CDS             <1..313
                     /locus_tag="VHR42_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3026748.1"
                     /translation="IDGSGEFRIFGKIILPSSKPLIAVLVIFTFMWRWNDFLWPLVIT
                     NDKTYYTVQLGLAMLVGDNYVNWNDLMMVSFAAMLPVVIVFFLFQKQFVQGVTTTGMK
                     A"
     gene            complement(382..>1537)
                     /locus_tag="VHR42_05935"
     CDS             complement(382..>1537)
                     /locus_tag="VHR42_05935"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014196172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX3026749.1"
                     /translation="VQMGINPFGFSWLLKPGESFQTPESVMVYSDAGLGQMSRRFHAF
                     YRERLCRGVYKDKKRPVLVNNWEATYFDFSETKIKEIAQEAADLGIELFVLDDGWFGH
                     RDDAKTSLGDWYVNKQKIPHGLDTLANEINQLGLKFGLWFEPEMVSPDSDLYRAHPDW
                     CIHAPERERSLGRNQLILDFSRKDVCDAITEMISHVLSTASVSYVKWDMNRYMTELGS
                     ALLAPERQSETAHRYILGVYRVIEELNERFPDILFESCASGGGRFDPGMLYYMPQTWT
                     SDNTDAVQRLKIQYGASLVYPIISMGSHVSATPNHQVGRITPLETRGRVAMSGNFGYE
                     LDLTELSQEEKQIVRNQVKRYKQIMRIHYMDNVTGVTGGKLKIPVFSHQK"
BASE COUNT          380 a          385 c          353 g          419 t
ORIGIN      
        1 catagacggt tccggggaat tcagaatttt cggaaagatc atacttccca gcagcaaacc
       61 gctgattgcg gttctggtca tttttacatt tatgtggcgg tggaatgact ttctttggcc
      121 gttggttatc accaacgaca aaacatatta tacggttcaa ctgggccttg ccatgctggt
      181 cggcgacaac tatgtaaact ggaacgattt gatgatggtc tccttcgcgg caatgctgcc
      241 ggtggtaatt gtattctttt tgttccaaaa gcagtttgtg cagggggtca cgacgaccgg
      301 catgaaagcc tgatccgcgc aggaattggg attgtgactt gaggaaagaa atatgataaa
      361 ttgtggaggc attgatgtcg gttatttttg atgagaaaaa acgggaattt tcagctttcc
      421 acccgtaaca ccagttacat tatccatgta gtggattcgc attatttgct tgtaacgctt
      481 cacttgattc cgaacaattt gtttttcctc ttgtgaaagc tccgtaagat ccagctcata
      541 cccgaaattc ccgctcatcg caacacggcc ccttgtttcc agcggagtga ttctacccac
      601 ctgatgattc ggcgttgcgg aaacatgcga acccattgaa atgatggggt agaccagact
      661 tgcgccgtat tgtattttaa gccgctgtac cgcatccgtg ttatcactgg tccaggtctg
      721 gggcatgtaa tatagcattc cggggtcaaa tctgccgccg ccacttgcgc agctctcaaa
      781 taaaatatcg ggaaaccgtt cgttcagttc ttcgattact cggtatactc ccaaaatgta
      841 ccgatgagcc gtttcactct gacgttccgg tgcaagcaaa gcggaaccca gctccgtcat
      901 ataccggttc atgtcccatt taacgtaaga taccgaagca gtggataaaa catgggagat
      961 catttccgtg atggcgtcgc aaacatcttt tctggaaaaa tcaaggatca gttgattgcg
     1021 gccaaggctt cgttcccgct ccggagcgtg aatgcaccag tccggatgcg cccggtacaa
     1081 atcgctgtcc ggcgatacca tttccggttc aaaccaaagc ccgaatttca gaccgagctg
     1141 attgatttcg tttgccagcg tatcaagtcc atgcgggatt ttctgcttgt tcacatacca
     1201 atccccaagg gatgttttgg cgtcatcccg atggccaaac cagccgtcgt ccaaaacgaa
     1261 cagttctatt cccaaatccg ccgcttcctg cgcgatttcc tttatctttg tttcactgaa
     1321 atcaaaatag gttgcttccc agttgttcac aagcacagga cgctttttgt ctttataaac
     1381 accccggcac aggcgttccc tgtaaaaagc gtgaaaccga cgggacatct gccccagacc
     1441 ggcatcggaa taaaccatta ccgattccgg ggtttgaaag ctttctcccg gtttgagaag
     1501 ccagctgaaa ccaaacggat taattcccat ttgtacc
//
LOCUS       DATALY010000204         9536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_130953,
            whole genome shotgun sequence.
ACCESSION   DATALY010000204 DATALY010000000
VERSION     DATALY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130953"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VHR42_05940"
     CDS             <1..835
                     /locus_tag="VHR42_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026750.1"
                     /translation="TAAPAPAPSSATESSSHETPAPSLPTSLTNAPTNATVDLSGAVL
                     PAGVTDVTFAVTPETASGAPTGAAGGTAGGASDPQGAAAFHIAVSDPALNVIGTPVLY
                     NIKLLDQNGNPISSFTGSVTVKLPIPAGLHGTPHVFRYEESTGALTDLGAAVQNGFLV
                     FSTTHFSYYIVAGTGDSVTLDTKNYQLPVGGTYQIGLKLTGGKAAFVKVTSTNGKAAT
                     AARLKNGNILATGKSTGTTYIMIDVYDRKNHLLTHASVRIDVKTGIRPRGDSTRQIGL
                     F"
     gene            900..1841
                     /locus_tag="VHR42_05945"
     CDS             900..1841
                     /locus_tag="VHR42_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3026751.1"
                     /translation="MIRTIVVDDEWYSLLEICELVEKTGIMSVEGQFQNGVDALREAE
                     FIRPQAAFIDIEMPEMDGFTLAEKLLESNPDIKIVFITGWNEYAVTAFEMNALDYIMK
                     PVNRTRFEKMAIHLQTELSTQKPSGKAALTIRCFGKFETLENGKPIAWKWAKAEELFA
                     LLVLNANTFVPKDVILKNLWPDYVSPKFLPIVKTSVCKIRNVLSDCVKSIRLTEEDGG
                     YGLFLSADVVCDYQNLWSTVHGFRAGDRRTYQQIYDACKIFQKGLLPSKEYPWVETYD
                     EKLWRGLADCLQLIANSLPEEKRSEALSLRPLNIQQQ"
     gene            1967..2554
                     /locus_tag="VHR42_05950"
     CDS             1967..2554
                     /locus_tag="VHR42_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX3026752.1"
                     /translation="MGKEFIHVRAIMKYVAFFRGINVGGKNLIKMDDLKQLFLDLGLS
                     KTKTYIQSGNVVFETDSEERPLLKTIQAGVVERFGLECTIILRNIKEMKTFIDQLPYS
                     QAEIAAAEAADTQVEHLYACFLEHLPEQTLLEELRHEYAGSDQLKMGQRDLYLLCRQS
                     IRNSKLAIRTANVFNSATVRNWKTVKKVYAMLASL"
     gene            2612..3199
                     /locus_tag="VHR42_05955"
     CDS             2612..3199
                     /locus_tag="VHR42_05955"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX3026753.1"
                     /translation="MTSVNILLFPDFEALDAFGPVEVLGCVPEYQLHYVSMRGGIVTS
                     RQGVRVLTESIQETDQSGILLIPGGQGTRPLVEEPKFIKSLKEIAVQAQYCLTVCTGS
                     ALLAMTGLLDHKKATSNKNAFEWVQSVNTNVQWMNRARWAVDQKYYTSSGVSAGIDMA
                     LGFVADQFEKDLAQKIAQNIEYIWNSNPEDDRFEK"
     gene            3189..3983
                     /locus_tag="VHR42_05960"
     CDS             3189..3983
                     /locus_tag="VHR42_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX3026754.1"
                     /translation="MKNEHTGQDHNGWEKPQKALGKIDEMLKIAVCDDEISAVQDLSS
                     KIEAFMDSNGLEFTLSKFSSGESLLSCIAAQNMYFDAVFLDIKMDRINGMDAAKKIRE
                     TNDKAMIIFVTALKEYVFDAFDVGAANYLVKPIEEKKLHHTLEKLAASVSLAAAHFLM
                     IHKNGEIVKIPCPDILYSEVVNHRVFIYERNSTYEYGAKIDDLGNQLTDDFFRCHRSY
                     LLNMKYVESYSDGFAYLTSGEKIPIATRRQPAFMKALLHYQRKEMG"
     gene            3985..5301
                     /locus_tag="VHR42_05965"
     CDS             3985..5301
                     /locus_tag="VHR42_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEX3026755.1"
                     /translation="MELFQTPLGFWYGFLFSLVLCGGVQALIQCYFLIRFSSVKSRPS
                     YYLIYIGISYSLLAAGLVLPIPENLPTLIGILVVFVFVNVILRQSKMTSVILSTLVVA
                     GTVLIESITTPLYALLSNSLKMNSIALNLSEGIVMLALTYLVLRFFAARYNLKTNEKS
                     KFLPVLALPLLFISIVLRLLLDFRYPPIFKDYQMTYQATAIEDFQFLFLAVTAFLCVC
                     GSLFAFEKVISYLETERANSILESQLSMQKSYAEEAKARYEATRAFRHDFKNHLIALS
                     GLIEKGETENAKKYLERFEQISQRMSFSVSTGNTAVDALLSEKLAYAENSGIHVELDA
                     AIPPTVTVDDFDLCAVFSNALDNAIKACGSAKNRDKILRITARPNKDFFLIDMSNTYE
                     PECVPKGTGIGLPVIKMIVEKYHGAIEISEENSVFFISMILPFNKK"
     gene            5467..6120
                     /locus_tag="VHR42_05970"
     CDS             5467..6120
                     /locus_tag="VHR42_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026756.1"
                     /translation="MNIAITSQTEGYGKTETDSQRSSLVDPYEQKKKKAADQLQRVGQ
                     NNVSEEVSVPDKVAKAEPSSDQFIRSDGSKTTSAGTYSLKKDGNGNSKIVYDKPTAGS
                     GTVNEGAHAKTSDKKGNPLVSPKKSEDKSNKAPANSSSNGGLQCTVDTDQVDSEIKNL
                     KKEKQQIEQEIKQSDGDEAKKQQLEEQLKEIDNELRTKDSDAYRKQHASYTYKSVQK"
     gene            complement(6137..7771)
                     /locus_tag="VHR42_05975"
     CDS             complement(6137..7771)
                     /locus_tag="VHR42_05975"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HEX3026757.1"
                     /translation="MSEPVYIAGHRNPDTDSVCSVIAYAELKKRLGVSAVPVRIGTIN
                     RETSFVLNYFGVEAPEYLESVRTQVSDLNMDIISPISADISVKAAWSIMQKSNVKVLP
                     VSNDDGRLMGIVTLSDITRSYMDALESNLLSAGNTPLHNITDTLKAKLVSGSEGSFRP
                     SGKVVIAAAEPDRMDSFIEKGDIVLAGNREDTQKKAVENGASCLVLTCGAKLDRAVLE
                     LAEKSGCVVMETDYDTFAAARLIDQSVPVGFIMTKDGLIRFHISDYIDSIKDKMLQTR
                     YRSYPVVDDDGFIKGFIARYHLISQRRKKIILLDHNEKSQTIDGIEQAEILEVIDHHR
                     IGDIQTENPVYFRNDAVGSTSTLIAGMYFEHGIRPPKSIAGILCAAILSDTLQFRSPT
                     STYADTSIAEKLAEIAGIQIDEFSAQMFEAGSALEGMSPEEILNYDFKEYTSGQLKFG
                     IGQVNSYHPQHMEQIRDSLLEHMRDVMDHGNYDFLLLLVTDLVNEGSFLLHVGDCGAL
                     IRKAFGVSAENSSVFLQGVVSRKKQIVPRLLNAIKV"
     gene            8025..8228
                     /locus_tag="VHR42_05980"
     CDS             8025..8228
                     /locus_tag="VHR42_05980"
                     /inference="COORDINATES: protein motif:HMM:NF040350.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6199 family natural product biosynthesis
                     protein"
                     /protein_id="HEX3026758.1"
                     /translation="MYVFWGVIVLAAGFFMLFAPKTCYDITQGWKNSADTDPSPLFLL
                     STRLGGIFFILAGTAAIVVSFLH"
     gene            8285..8695
                     /locus_tag="VHR42_05985"
     CDS             8285..8695
                     /locus_tag="VHR42_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3026759.1"
                     /translation="MENSKPDNLLFMDFLENYNQAFYDKDINKLKDFYDSAGSVLIYF
                     DNHKNNDTFCLEDHLRLISDFFKNGKATESGKVEPISIENLHIFSKTDSACLCFIVRY
                     QSFPVPAVRATMYLERIGGTWKILHVHCSFEPEK"
     gene            8760..9437
                     /locus_tag="VHR42_05990"
     CDS             8760..9437
                     /locus_tag="VHR42_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3026760.1"
                     /translation="MINILIIEDDEEINNLLCSILCKEKYICKSIYSGLQALAAMKAD
                     RFDMVLLDLMLPGISGEELLRDFRRFSNVPVIIISAKDETAVKVDMLRTGADDYITKP
                     FDNNEVLARVESSLRRFQGYTSPLLIAGNLEIDTEKNICSVSGQTVSLTGIEIKILRL
                     FMEHPKKLFTKSNLYGSVWNEPYDYDDNAVNTHISNLRRKLKSVCPDQEFIETVWGIG
                     YKLAGPT"
BASE COUNT         2569 a         2325 c         2265 g         2377 t
ORIGIN      
        1 aacagcagct cctgcgccgg cgccctcatc tgcaacagaa tcctcctcgc acgaaacacc
       61 cgcaccttcc ctgcccacct ctctgacaaa tgcaccgacg aatgcaacgg ttgatctttc
      121 cggcgcggtg ctgccggcag gcgtaaccga tgtaacgttt gcagtcaccc cggaaacagc
      181 ctccggcgct ccgacaggag cagccggtgg aacagcaggc ggagcctccg acccgcaggg
      241 cgcggcggct ttccatattg ccgtctccga tcccgccctg aacgtcatcg gcacaccggt
      301 actttataac atcaaactgc tcgaccagaa cggaaatcca atttcctcct ttaccggcag
      361 cgtaacggta aaactgccga ttccggcggg tctgcacggc acaccgcacg tattccgcta
      421 tgaggaaagc accggggctc tcaccgatct cggtgcggcg gtgcaaaacg ggtttctggt
      481 attctccaca actcatttca gctattacat tgtagcgggt acgggcgatt ccgtcacgct
      541 ggacacaaaa aactatcagc tgcccgtagg cggaacgtat cagatcgggt taaagctgac
      601 cggcggcaaa gctgcttttg tgaaggttac ttctaccaac ggcaaagctg caaccgcagc
      661 acggctgaaa aacggcaata ttctggcaac gggcaagagc accggtacga catacatcat
      721 gatcgacgta tacgaccgaa aaaatcacct gctgacccac gcctccgttc ggatcgacgt
      781 caaaacaggc atccggccga gaggcgattc cacaaggcag atcggcctat tttaaccgaa
      841 aaaggcgcta attaattcaa tcaattccgt ctgaaaaagt cccatggagg ctgcacaaaa
      901 tgatacgaac aattgtggtt gacgacgagt ggtacagcct tctcgaaatc tgcgaattgg
      961 tggaaaagac tggcatcatg agtgtggaag ggcaatttca aaacggagtc gacgccttgc
     1021 gggaagccga gtttatccgc ccgcaagcgg cgtttatcga cattgaaatg cccgaaatgg
     1081 acggttttac gcttgcggaa aaacttctgg aatccaaccc agatattaaa attgtcttta
     1141 ttacaggttg gaatgagtac gccgtaacgg cctttgagat gaacgccctg gattatatta
     1201 tgaagcccgt caaccgaacc cgctttgaga aaatggcgat acacctgcaa accgagcttt
     1261 ccacgcaaaa gccttccggc aaagccgcac tgaccatacg ctgctttgga aaattcgaaa
     1321 cgctggaaaa cggaaagccg attgcctgga agtgggccaa agcagaggag cttttcgctc
     1381 tgttggtgct gaacgccaat acttttgttc cgaaagacgt catactgaag aacctgtggc
     1441 cggattatgt cagcccaaaa tttcttccaa tcgttaagac ctccgtttgc aaaatcagaa
     1501 acgtcctttc agactgcgtg aaatccatcc gtctgaccga ggaagacggc ggatacggat
     1561 tgtttctgtc tgcggacgtc gtctgcgatt atcagaatct ttggagcaca gtgcacgggt
     1621 tccgggcggg cgaccgcaga acttaccagc agatctacga cgcctgtaaa atctttcaaa
     1681 agggtctgct tccgtccaaa gaatacccat gggtcgaaac ctatgatgaa aagctttggc
     1741 gcggtctggc ggattgtctc caattgatcg cgaattcttt gccagaggaa aaaagatcgg
     1801 aggcgctcag ccttcgtccc ctgaacattc aacagcagta aaatgaatcg tctcagtaag
     1861 ggcgcgatca gacgaagaag tctgaccgcg cccttactgt ttcaaaaggc atgttccgcc
     1921 ttgctgttcc gctcgattat tgtatttatt ttttccgagg gatataatgg ggaaagaatt
     1981 tattcatgtg agggcgatta tgaaatatgt ggcgtttttc agaggaatca acgtcggtgg
     2041 taaaaatcta attaaaatgg atgatttgaa gcagcttttt ctcgatttgg ggctgtccaa
     2101 aacaaaaacc tatattcaaa gtggcaacgt tgtcttcgaa acggattccg aggaaaggcc
     2161 gctgctgaaa acgatccagg ccggtgttgt ggagcgcttc gggctggagt gcacgatcat
     2221 ccttcggaac atcaaggaaa tgaaaacatt cattgatcaa cttccctact cccaagctga
     2281 aattgctgct gccgaagccg ccgatacaca ggtagaacac ctgtatgcct gctttctgga
     2341 gcatctgccc gagcaaacac tgctggaaga actccgccat gagtatgccg gttcggacca
     2401 gctgaaaatg gggcaacggg acctgtatct tctttgccgc cagagcatcc gcaattcgaa
     2461 gctggcgatt cgtaccgcca atgtgtttaa ttccgccacc gttcgaaact ggaaaaccgt
     2521 aaaaaaggtt tatgctatgc tggcctctct gtaaatttgg taattcaggg aactgctgcc
     2581 agcacagcat gcaataaagg aggacgatat tttgacaagt gttaatattt tgttgttccc
     2641 ggacttcgaa gcgctggacg ctttcggccc ggtagaggtt ctggggtgtg ttccggagta
     2701 tcagctccat tatgtatcga tgagaggcgg catagttacc agccggcagg gtgtgcgtgt
     2761 gttaacggaa agcattcagg agaccgatca atccggaatt cttctgattc ccggcggaca
     2821 gggaacgcgg ccgctcgtcg aggaaccgaa gtttattaaa agtctgaaag agatcgcggt
     2881 gcaggcacaa tactgcctga cggtgtgcac tggttcggca ctgctcgcaa tgaccggcct
     2941 tttggatcat aaaaaagcta catcgaataa aaatgccttt gagtgggttc agtcggttaa
     3001 taccaatgta cagtggatga atcgcgccag atgggctgtc gatcaaaaat actacacttc
     3061 atcgggggtc tccgccggga ttgatatggc gttgggcttc gttgccgatc aatttgaaaa
     3121 agatctcgct cagaaaatcg cccaaaacat cgaatatatc tggaactcca acccggagga
     3181 tgaccggttt gaaaaatgag cataccgggc aggaccataa tggatgggaa aagccacaga
     3241 aagcattggg aaagattgat gaaatgctta aaatagcggt atgtgacgat gaaatatccg
     3301 ctgttcagga tttaagcagt aaaatagaag cgtttatgga ctcgaatggt ttggaattta
     3361 ccttgtcgaa gttttccagc ggcgaatctt tactttcctg tattgccgcc caaaacatgt
     3421 attttgacgc tgttttcctt gatattaaaa tggatcggat caacggcatg gatgccgcca
     3481 aaaagattcg cgaaaccaac gacaaggcca tgataatttt tgtgacggcc ctcaaagagt
     3541 atgttttcga cgcctttgac gtcggcgcgg caaactatct tgtaaaaccg attgaagaga
     3601 aaaaattgca tcacaccttg gaaaagctgg cggcgtccgt ttctttggct gccgctcact
     3661 ttcttatgat ccataaaaac ggtgagatcg taaaaattcc ttgtccggat attctgtaca
     3721 gcgaggtggt aaaccaccgc gtttttattt atgagcggaa cagcacttat gaatacggag
     3781 cgaaaatcga cgatctgggg aaccaattga cggatgattt tttccgatgt cacaggagct
     3841 atcttctgaa catgaaatat gttgagagct attccgatgg ctttgcttac ctgacgagcg
     3901 gcgaaaaaat ccccattgcc acccggaggc aaccggcttt catgaaggct ttgctgcact
     3961 atcaaagaaa agagatgggt tgaaatggag cttttccaaa cacctcttgg cttttggtat
     4021 ggatttctct tttcgctggt gctttgcggc ggagtgcagg ctttgattca gtgctatttt
     4081 ctcattcggt tctcgtcggt aaaaagcagg ccgtcttatt atttaattta catcggaatt
     4141 tcctattccc ttctggccgc ggggctagtc cttccgattc cggagaatct tccgacctta
     4201 atcggaatcc tcgttgtatt cgtttttgta aacgtcatcc tcaggcaaag caagatgacg
     4261 tccgtaattc tttcaaccct ggttgttgcc ggcaccgtac tgattgagag tataacaacg
     4321 cctttatacg ctctgctgtc aaacagtttg aagatgaatt ccatcgcgct gaatctatcc
     4381 gagggaatcg tcatgcttgc actgacgtat ctggtgctcc gtttcttcgc ggctcgatac
     4441 aatctgaaaa ccaacgaaaa atcgaaattt cttccggttc tcgctttacc gttgcttttt
     4501 atcagcatcg tactgcggtt attgctggat tttcggtatc cgccgatctt taaagattat
     4561 cagatgacct atcaggcaac ggcaatcgaa gattttcaat ttctgtttct ggccgttacc
     4621 gcgttccttt gcgtgtgcgg ttccctgttt gcgtttgaaa aggtaatcag ctatcttgaa
     4681 acggaacggg caaattcaat tctggagtca cagctttcca tgcagaaaag ttatgcggag
     4741 gaagcaaagg cgagatatga agccacccgc gcgtttcggc acgatttcaa aaaccatctc
     4801 attgcgctct caggcctgat tgaaaaggga gaaacagaaa acgcgaaaaa atatctggaa
     4861 cgtttcgaac agatttctca acggatgtcc ttttccgtca gcaccggcaa tacggcggtg
     4921 gatgcgctgt taagcgagaa attagcctat gcggagaatt ccggcattca tgtggaactt
     4981 gacgccgcca ttcctccgac ggtaaccgtt gatgatttcg atttatgcgc tgttttctcc
     5041 aatgctttgg ataacgcaat caaggcctgc ggcagcgcaa aaaatcggga taaaatactc
     5101 cgcataacag ccagaccgaa caaagacttc tttctgatcg atatgagcaa cacgtatgaa
     5161 cccgaatgcg ttccgaaagg cacgggaatc ggattaccgg taatcaaaat gattgtagaa
     5221 aaataccacg gggcgattga aatctcagaa gaaaacagcg tttttttcat cagtatgatt
     5281 ctgccgttca acaagaaata aagcatccgc agggtaaaac gcaagcccgg tcattcccaa
     5341 gctttaccgc ttctggatga ccgggctttg cgttcacgct ccaaatggca gcgcctgcga
     5401 tgttttgctg gaataggaca cctgccggaa cgataaataa gtaaatgcga atggaggtag
     5461 atcgatatga atatcgcaat tacaagccaa accgaaggat acggcaaaac cgaaaccgac
     5521 agtcagcggt cctcactggt tgatccgtat gaacagaaaa agaagaaagc agcggaccag
     5581 ttgcagcgtg tgggacaaaa caatgtcagc gaagaagttt ccgtaccgga taaggttgcg
     5641 aaagccgaac cgtcgagtga ccagtttatc cggagcgacg gctcaaaaac cacctcagcc
     5701 gggacttaca gcctaaaaaa agacggaaac ggcaattcta aaatcgtcta tgacaaaccg
     5761 acggcgggtt ccggtactgt caacgaaggc gctcatgcaa aaacatccga caaaaagggg
     5821 aatccactcg tttccccgaa gaaatcggag gacaaatcaa acaaggcacc ggcgaattcc
     5881 tcctccaatg gcgggctcca atgtacggta gataccgatc aggtcgattc ggaaattaaa
     5941 aatctaaaaa aagaaaagca gcagattgaa caggaaatca agcaatccga cggagatgaa
     6001 gcgaagaaac aacaattgga agaacagtta aaagaaatcg acaacgaact tcgtaccaag
     6061 gattctgacg cgtatcggaa acagcatgcg tcttatacct acaaatccgt tcagaaatag
     6121 gaaccgactg tttcgtctac accttgattg cgttgagcaa gcgcggcacg atctgttttt
     6181 tccttgacac gaccccttgc aggaaaacgg agctgttttc ggccgacact ccaaacgctt
     6241 tgcggatcag cgcgccgcaa tccccaacat gcagcagaaa agagccctcg ttgacaaggt
     6301 cggtcaccaa aagcagcagg aaatcatagt tcccgtgatc cataacgtcc cgcatatgct
     6361 ccagaaggga atcccgaatc tgctccatat gctgcggatg ataggaattg acctggccga
     6421 ttccgaattt gagctgcccg gacgtatact ccttaaagtc gtaattcaag atttcctccg
     6481 ggctcatgcc ttccaatgcc gaacccgctt caaacatctg cgccgaaaat tcgtcgatct
     6541 gaatcccggc gatttccgca agcttctccg caatcgaagt atccgcatag gtgctcgtgg
     6601 gcgaccggaa ctgcagcgta tcggaaagga tcgcggcgca cagaatccct gcgatcgatt
     6661 tcggcggccg gattccgtgc tcaaaataca ttcccgcaat cagcgttgac gtgctgccca
     6721 cggcgtcgtt cctgaaatac accggatttt ccgtctgaat atccccgatc cggtggtggt
     6781 cgatgacctc cagaatctcc gcctgctcga ttccgtcaat tgtttggctc ttttcattat
     6841 gatcgagtaa aatgatcttt ttccttctct gggaaatgag atgatacctt gcaataaagc
     6901 ctttgatgaa cccgtcgtcg tccacaaccg ggtagcttct gtaccgcgtc tgaagcattt
     6961 tatctttgat cgaatcgata tagtcgctga tatggaagcg gatcagtccg tctttcgtca
     7021 tgataaagcc aacaggaaca ctctgatcga tcagtctggc ggcagcaaag gtgtcatagt
     7081 cagtttccat cacgacgcat ccgcttttct cagcaagctc cagaacggcc cggtccaatt
     7141 ttgcaccgca ggtcaaaaca aggcagctcg caccgttttc tactgccttt ttctgcgtat
     7201 cctcccgatt cccggccaga acaatatcgc ccttttcaat aaatgagtcc atcctgtccg
     7261 gttccgccgc ggcaattacg acctttcccg acgggcggaa gctcccttcg ctcccggaaa
     7321 ccagttttgc cttcaatgta tccgtgatgt tatgcagcgg cgtattaccc gctgaaagca
     7381 gattgctttc cagcgcgtcc atataactgc gggtgatgtc ggagagagtc acaatgccca
     7441 tcaacctgcc gtcgtcgttg gaaaccggca aaaccttgac gttgcttttc tgcataatgc
     7501 tccaagcggc ttttacggaa atatccgccg aaatcgggct gataatatcc atattcaggt
     7561 cggaaacctg cgtccgcacc gattccagat attccggcgc ttcaactcca aaataattca
     7621 gaacaaaact ggtctcccgg ttaatagttc cgatccggac ggggacagcg gaaacaccga
     7681 gcctcttttt aagctccgca taggcgataa cggaacaaac tgaatccgta tccgggttcc
     7741 tgtgtcctgc aatatacacc ggctctgaca tggcgcataa cctccaaatt aaacggatgc
     7801 tgattctttg tgtttatttt accacgctta aggctaaatt tgttactcaa tcgggttttc
     7861 gcccctattt ctataaaatt tcgcctttca ggctttcggt ttctccccat ttttcattga
     7921 gcaacctggg aaaattttta tcgtcctagc tttttttata tgccgtctct gcaattccat
     7981 gatatactga atttagaata acttcaggaa gccggtgaaa agccatgtat gtgttttggg
     8041 gcgtgatcgt attggcggct ggttttttta tgctgttcgc cccaaaaaca tgttatgata
     8101 ttacacaggg gtggaaaaac agtgcggata cggacccatc ccctctgttt ctcctcagta
     8161 cgaggctcgg cggcattttc ttcattcttg ccggaactgc ggctattgtc gtttcgtttc
     8221 tgcattagcg ttggcaaaaa gagcgctgta ttttttccca actaagttgc cgtgaggtgt
     8281 tgttatggaa aattccaagc ctgacaacct gttatttatg gattttttgg agaactataa
     8341 ccaggccttc tatgataagg acatcaataa gctaaaagac ttctatgatt ctgccggtag
     8401 cgtccttata tacttcgaca atcataagaa taacgatacc ttctgcttgg aggatcatct
     8461 gcggctcata tccgactttt ttaagaacgg aaaggcaacg gagtccggaa aagtagaacc
     8521 catcagcatc gagaatttgc atatcttttc aaaaacagat tcagcctgcc tatgttttat
     8581 cgtcagatac caaagctttc ctgttcccgc agttcgagct acgatgtatc tggaacgcat
     8641 cggcggcaca tggaaaattc tccatgtgca ctgttctttc gagccggaaa aatagccggg
     8701 atgaattatc cgtttgattg cgcagtgcaa aaaagctctg aaatataggg gacaatcaaa
     8761 tgattaacat tctgattata gaagacgatg aagaaatcaa caatttatta tgcagtattc
     8821 tttgcaagga aaaatacatt tgcaaatcca tctattccgg tttgcaggca ttagcggcaa
     8881 tgaaagcaga ccgatttgat atggtgctgc tggatttgat gctgcccgga atttccggtg
     8941 aagagctgct ccgggatttt cggcgctttt ccaatgttcc ggtcattatt atctccgcga
     9001 aggacgaaac tgccgtgaaa gtcgatatgc tccgcacggg agctgacgat tacatcacaa
     9061 agcccttcga caacaacgag gttctggcaa gggtggaaag cagcctgcgg cgctttcagg
     9121 ggtatacctc cccgctgctg attgccggca acttagagat cgataccgaa aaaaatatct
     9181 gctccgtttc cggccagaca gtttccttaa ccggcattga aatcaaaatt ctccggcttt
     9241 ttatggaaca tcccaaaaag ctcttcacaa aatccaatct gtacggcagt gtctggaatg
     9301 aaccgtatga ttacgacgac aatgcagtca acacgcacat cagcaattta agacggaagc
     9361 taaaatccgt ctgcccggat caggagttta tcgaaaccgt ttggggcatc ggttataaac
     9421 ttgccggtcc aacatgaaac acctgttttc gttcgatacg gaaacaggtg tttttatcct
     9481 gatctttaga aacacttaag aattgcgatt tacaataggc ggtaaaggag atggaa
//
LOCUS       DATALY010000205         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_477189,
            whole genome shotgun sequence.
ACCESSION   DATALY010000205 DATALY010000000
VERSION     DATALY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477189"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHR42_05995"
     CDS             complement(<1..363)
                     /locus_tag="VHR42_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX3026761.1"
                     /translation="MGKHRSPFIGLVRPGNKTIHDMPTFQFCRAESCRLKNPAAAAEK
                     WIVMTLAICPGSFDPVTLGHYDVIKRASALFDKVIVVVMVNSTKRPAFTVEERVDFIR
                     RTVDSLPNVEVDTYTGLLA"
     gene            complement(314..862)
                     /gene="rsmD"
                     /locus_tag="VHR42_06000"
     CDS             complement(314..862)
                     /gene="rsmD"
                     /locus_tag="VHR42_06000"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX3026762.1"
                     /translation="MRVITGLSRGSKLITLEGMDTRPTTDRVKESIFNMIQFELEGRK
                     VLDLFAGSGQLGIEALSRGAESAVFIDQNPEAVKVIRQNLLHTKLAEKAKVLASDYAS
                     YLSHATTEFDIALMDPPYRQGLATAALPMVAALMSRYGVIVCETAAGEELPEQAGDFE
                     LSRRNVYGKASIAVYRFGKARE"
     gene            complement(964..>2713)
                     /gene="uvrC"
                     /locus_tag="VHR42_06005"
     CDS             complement(964..>2713)
                     /gene="uvrC"
                     /locus_tag="VHR42_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX3026763.1"
                     /translation="IGKAKALKKRVSQYFLRFDHHEGKVRKMVEHVDHFDTIITDSEF
                     EALVLECSLIKQHKPKYNILLKDDKGYSYIRITLDEQWPRIYSVKRKDEDGATYLGPY
                     ISSFAVKEAVDEAVKAFALPVCGRRFPQDIGKGRPCLNYFIRQCCAPCRGKISNAEYR
                     ETIENAVLFLRGGYGLILEQLEDKMNQASENLEFEKAARMRERITAIKKIREKQKVVA
                     SRVEEQDVVAAAGDGCATVFEVFLIRSGRLTDREEFVVDYLQDEKAARAEFIQGYYTL
                     RKKAPKQITLDGETEDRGLLEEYLTRMSGRKIRITVPQKGGQKNLAVMARTNACEKLS
                     QLGARPGREEKTLEELQALLGMKQTPSYIEAYDISNTAGSGMVAGMVVFENGQPKKSA
                     YKKFSVKSVTGPDDYASMREVVGRRLARLREAVDQGRTESAGFARVPDLILLDGGRGH
                     VAAVKPVLEEYGFDIPLFGMVKDNSHRTRAIAQNGGEISINSNRQVFTLISKIQDEVH
                     RFAIAYHHKTQSRKGLSSALIGAPQIGEKRARALMRHFKTMEKIRKASIDELSEADGM
                     NHRAAAALKVWLENQP"
BASE COUNT          613 a          782 c          609 g          709 t
ORIGIN      
        1 cgcaagcagg ccggtatatg tatcgacttc gacattcggc aaactgtcca ctgtccttcg
       61 gataaaatcg actctttctt ctacggtaaa agccggtctt tttgtagaat tcaccatcac
      121 caccacaatt actttgtcaa acagagccga tgctctttta ataacatcat agtgccccag
      181 cgtaaccgga tcaaaactgc cgggacagat tgccaacgtc ataacgatcc atttctccgc
      241 cgccgctgcg ggatttttca gcctgcaact ttccgcgcgg cagaattgga aagtcggcat
      301 atcgtgaatt gttttattcc cgggccttac caaaccgata aacggcgatc gatgctttcc
      361 cataaacatt tcttcggctg agctcaaaat cccccgcctg ttcgggaagt tcttcccccg
      421 cggcggtttc acagacaatg acaccgtagc gagacatcag agcagcaacc atcggcagcg
      481 cagctgtcgc aaggccctgc ctgtaagggg ggtccatcag ggcaatgtca aactccgttg
      541 tggcatggct gagataagaa gcgtaatcgg aagccagaac cttggctttc tccgccagct
      601 tagtgtgaag aagattctgg cggatcactt taacagcttc cggattctgg tcaataaaaa
      661 ccgccgattc ggctccccgg ctcagcgcct ctatccccag ctggccgctg ccggcaaaaa
      721 gatccagcac cttccgtccc tccagctcaa attgaatcat attaaaaata gattccttaa
      781 cccggtcggt tgtcgggcgg gtgtccattc cttccagcgt tatgagcttt gatccacggg
      841 ataatcctgt aattactctc atgctaacct ccagccgaag tgtttctttc catattatcc
      901 aaccaatttc gacaattgtc aactattttg gataaaatct gtttcgttca acttattgta
      961 ttattaaggc tgattttcaa gccaaacttt cagcgcagcg gcggccctgt gattcattcc
     1021 gtccgcttcg gaaagctcgt caatcgacgc ctttcgaatc ttttccatcg tcttaaaatg
     1081 cctcatcagc gctctggcac gcttctcccc gatctgggga gcgccgatca gcgcagatga
     1141 taaccctttc cggctctgcg tcttatggtg gtaggcgatc gcaaaccggt gaacctcatc
     1201 ctgtatctta gaaatcagag taaatacctg acggttggaa ttaatggaaa tctctccgcc
     1261 gttctgcgca atcgcccttg tgcgatggga attgtccttg accatgccga acagggggat
     1321 atcaaagccg tattcctcaa gaacaggttt gacagccgcg acatggcccc ggcctccgtc
     1381 aagcaggatc agatccggaa ccctggcaaa accggcggac tccgttctgc cctgatcaac
     1441 ggcctcccgg agacgggcaa gccgccggcc aaccacttca cgcatggatg cgtagtcatc
     1501 aggacccgtt accgacttga cggagaactt tttataggca ctttttttcg gctgaccatt
     1561 ttcgaaaacc accatgcccg cgaccattcc tgatcccgcg gtattggaga tatcgtatgc
     1621 ttcaatataa gagggagtct gtttcattcc aagcagcgcc tgcagctcct caagtgtttt
     1681 ttcctccctg ccgggacgtg cgccaagctg gctcagcttt tcgcatgcgt ttgttctggc
     1741 cattacggca aggtttttct gtcccccctt ctggggcacg gtaattcgga ttttacggcc
     1801 ggacattcgg gtcagatact cctcgagcag ccccctgtcc tccgtttcac cgtcaagcgt
     1861 tatctgtttg ggtgccttct tgcggagggt ataataaccc tgtataaatt cggcccgggc
     1921 ggccttttca tcctgaaggt aatccaccac aaactcttcc cggtcggtaa gcctcccgct
     1981 gcggatcaga aatacttcaa acaccgttgc gcatccgtcc ccggcggcgg cgacaacatc
     2041 ctgctcctca acccgggagg caaccacctt ttgtttctcc cggatctttt tgatggccgt
     2101 gatccgttca cgcattctgg cagccttttc aaactcaaga ttttcggacg cctgattcat
     2161 tttatcctca agctgctcca gaatcagacc atagccgccg cgcagaaaca ggacggcgtt
     2221 ttcaatggtt tcccggtatt cggcattgct gatttttccc cgacagggcg cgcagcattg
     2281 ccgaatgaaa taattcaggc agggcctccc tttcccgata tcctgcggaa aacgccgtcc
     2341 gcaaaccggc agcgcaaaag ccttgaccgc ctcatcgact gcctctttca ctgcaaacga
     2401 actgatatag ggcccaagat aagtcgcccc gtcttcatct ttgcgtttca cagaataaat
     2461 tctcggccac tgctcatcga gggtgattcg gatataacta tagcccttgt catctttaag
     2521 aagaatgtta tatttgggtt tgtgctgttt gatcaggctg cattccagca ccagcgcttc
     2581 aaattcactg tcggtaataa tcgtatcaaa atgatcgaca tgttccacca ttttacgaac
     2641 ttttccctca tgatggtcaa atcgcagaaa atactggctg acgcgttttt tgagcgcctt
     2701 ggctttcccg ata
//
LOCUS       DATALY010000206         6642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_131303,
            whole genome shotgun sequence.
ACCESSION   DATALY010000206 DATALY010000000
VERSION     DATALY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131303"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VHR42_06010"
     CDS             complement(<1..975)
                     /locus_tag="VHR42_06010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3026764.1"
                     /translation="MRSSVIKEGPARASHRSLLKALGITDSEMKRPFIAVVSSASDYI
                     PGHMHLKNISEAVKAGIRSAGGVPFEFNTIGVCDGISMNHEGMKYSLCSREIIADSIE
                     VMLTAHPLDAAVFIPNCDKIVPGMLIAAARLNIPSVFVSGGPMYAGRFRGARVGLNEV
                     FEAVGSYAAGKMTDDDLYELEEKACPGCGSCSGMYTANSMNCLTEALGMALPGNGTIP
                     AVKSARIRLAKETGERVLELLRENIRPLDILTEKAFENGIRTDMALGCSSNTVLHLTA
                     VAHQAGYEIEMSKFDEIGHSTPQICKLNPAGTDFIEDLDEVGGINAVMK"
     gene            complement(1019..1651)
                     /locus_tag="VHR42_06015"
     CDS             complement(1019..1651)
                     /locus_tag="VHR42_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026765.1"
                     /translation="MTKSNLREKISNYWYYYKVHTYVGILVLISLVFMITQCAKKVNP
                     DGTAMLLIKDTVLETAQSKQIDGVLSRYSQDLNHDGQHYIMLDTLDINPKFTQLETAN
                     EIKLAANFQTGDEVLVLMDQAGYGRLSDKGVFQNVKSLVPNAVTVDDFRVRLSDIPAF
                     RELKIPAVTDRLYLSIRKYAGAVKAATNRTNYDNSVAIFKELLKNQKSSG"
     gene            complement(1685..3187)
                     /gene="lysS"
                     /locus_tag="VHR42_06020"
     CDS             complement(1685..3187)
                     /gene="lysS"
                     /locus_tag="VHR42_06020"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX3026766.1"
                     /translation="MSEEIENNVNQTQEEFNEILRIRREKLKELQANNKDPFGITTYD
                     RDKRNSEIAEHFDELDGKEVSIAGRMMSRRDMGKANFIDVHDATGRMQVYVRINDVGE
                     EPYAEFKKWDIGDIVGIRGIVFRTRRGEISIHANEITLLSKSLLPLPEKWHGLKDTEL
                     RYRQRYVDLIVNPEVKDTFIKRSRIISCIRSFLDAQNFIEVETPILNVIPGGATARPF
                     ITHHNTLDIDLYLRIATELYLKRLIVGGMERVYEMGRIFRNEGMSIKHNPEFTTIEIY
                     QAYTDYKGMMKLAEDMIAHVAKEVTGSTVITYQGDEVDLTPPWRRMTMAEAVKTYTGA
                     DFMSFRGDHEKARAVAKDMGIECGEDKTWGDALYMIFDEKVEENLNEPTFILDYPIEV
                     SPLSKRKQDMPELTERFEFFITRREMGNAFSELNDPIDQKERFLRQVQLREAGDQEAG
                     MMDEDFITALEYGMPPTGGIGIGIDRLAMLLTDSYSIRDVLLFPTMKPRD"
     gene            complement(3333..3806)
                     /gene="greA"
                     /locus_tag="VHR42_06025"
     CDS             complement(3333..3806)
                     /gene="greA"
                     /locus_tag="VHR42_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX3026767.1"
                     /translation="MAKQVILTDEGLRKLEDELEQLKTEKRKEIAEKIKVALSFGDLS
                     ENSEYDEAKNEQATVEIRIAQIEAMLKNVKLIDDDEINTEIVNLGSKVTVLDLEFNEE
                     ISYQIVGSTEADPDINRISDESPVGRSLLSHRVGDVVDVETPGGVVQFKIQMISK"
     gene            complement(3937..4779)
                     /locus_tag="VHR42_06030"
     CDS             complement(3937..4779)
                     /locus_tag="VHR42_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026768.1"
                     /translation="MFAAVTFLPCCHDYQRFGFLERFLAPKPRLFEISNGVISILDIE
                     SSLDSRGQPRWNRILSFARKNGINKYLLPAGMPCPEAVKPMICSPEQYLRNYCLRMSV
                     KAAKDAGLVLSELDTAILDTNGYSNGHLAAEAIPVFRSVTIYTQKEWKYEFFRSLLAD
                     DRKDSLKYTQNQDGLARCHIILAPYGVEGFDPHGLPGLTVSAKPVPESWEGPVIDGFE
                     PEIPEILESIRPPEIDKLAFAAALWQHAELAPLDSLPLVRCLSRGDPIDCDLITSGLS
                     RLDS"
     gene            complement(4987..6180)
                     /locus_tag="VHR42_06035"
     CDS             complement(4987..6180)
                     /locus_tag="VHR42_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3026769.1"
                     /translation="MNKKISVGAAVMLMLMTAAVAVTITMIFSMKMINKNLTDFSARE
                     SMFKKLADVDAKVRERYVGKIDETKLEDELINGYMKGISDKYGTYLDAEDYKKIETDI
                     QGKGIGIGVNVIENAEGYINVVSVVKKSPAEAAGVLAGDVIVKIGSEDVKALGYASAV
                     NLLKGDAGTKAEFTVKRNGVEKPFSIVRKQFESQSVESRMIGNLAYVRILEFDSNTDE
                     QFSAQVDNLVKKGAKGIVFDVRNNPGGLLDTVEKMIDKLVPAGPVVRAKYKTGSISTL
                     YRSDAAQISLPMAVLTNQNTASAAELFTAALKDYGKAKSIGTKTYGKGTMQTIFDLND
                     GTALDLSIAYFYPPKSNNFEGKGVTPDIEVDLSSAKLSRFYQLSDQEDDQLQAGLNYV
                     KTLIQ"
     gene            complement(6234..>6642)
                     /locus_tag="VHR42_06040"
     CDS             complement(6234..>6642)
                     /locus_tag="VHR42_06040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3026770.1"
                     /translation="WPTTVRRITDSYGWRTHPITGIWGFHDGIDIAASMGSPIYATDS
                     GTIVLQKFAKLAAKDIVYYYGDNYVFINHHNGIVSFYGHCSARASGIKEGASVQRGQV
                     IAYVGSKGKSTAAHLHFGVYVNGKAQNPLNYLH"
BASE COUNT         1568 a         1747 c         1446 g         1881 t
ORIGIN      
        1 tttcatgact gcgttaatcc cgccgacctc gtcaagatct tcaatgaaat cggtgcccgc
       61 cggattcagt ttgcagatct gcggggtcga atggccgatt tcgtcaaatt tgctcatttc
      121 aatttcgtac cctgcctgat gggcgacagc cgtcagatga agcactgtat ttgaggagca
      181 gcccagcgcc atatcggttc ggatcccgtt ctcaaacgcc ttttccgtca ggatatccag
      241 gggcctgata ttttcgcgta aaagctccag cacccgctcc cctgtttcct tggcgagtct
      301 gattctcgcg cttttgaccg ccggtatggt tccgttgccc ggaagggcca ttcccagagc
      361 ctcagtcaga cagttcatcg agtttgccgt atacatgccc gagcaggagc cgcagcccgg
      421 acaggctttt tcctccagct cataaagatc atcgtccgtc atcttcccgg ctgcatagct
      481 tccaactgcc tcaaacactt cattcaggcc taccctggct cctctgaagc gaccggcata
      541 catcggccct ccactgacaa aaacactggg gatattcagc cttgccgcag caatcagcat
      601 acccggcacg atcttgtcgc agtttggaat gaacacggcg gcgtcaagag gatgcgccgt
      661 cagcatgact tcaatcgagt cggcgataat ttcccgcgag cagagtgaat atttcattcc
      721 ctcatgattc atggaaattc cgtcacagac tccgatggta ttaaattcga acggaacgcc
      781 gccggccgac cgaattccgg cctttaccgc ttccgatata ttcttcagat gcatatggcc
      841 cgggatgtaa tcgctcgcag aggaaaccac ggcgataaac ggccgtttca tctcgctgtc
      901 cgttattccc agagccttta acagcgacct gtgagatgcc ctcgcggggc cttcctttat
      961 cacactgctt ctcacgatat gaacaatcct ttcccgcagg cggtgaaaag ccgcctgatc
     1021 atccgcttga tttctggttt ttgagcaact ctttgaatat tgcaactgaa ttgtcatagt
     1081 tggtcctgtt tgtggccgcc ttgacagccc ctgcgtattt gcggatagac agatacagcc
     1141 gatcggtaac cgccggtatc ttcagttccc ggaaagccgg gatgtcgctg agtctcaccc
     1201 ggaaatcgtc aactgttact gcattcggca caagactttt tacgttttga aaaacaccct
     1261 tgtcggaaag tctgccatag ccggcctgat ccatcagaac cagtacctca tctccggttt
     1321 gaaaatttgc cgcaagcttg atttcgtttg ctgtctcaag ctgggtgaat ttcggattga
     1381 tgtcgagagt atcaagcata atgtaatgct gtccgtcatg gttaagatct tgtgaataac
     1441 ggctcaggac accgtcgatc tgcttggact gcgccgtttc gagtaccgtg tctttgatca
     1501 gcagcatagc cgtcccatca ggatttacct ttttggcgca ttgggtaatc ataaaaacaa
     1561 gcgaaatcaa cactaagatt ccgacgtaag tgtgaacctt ataataatac cagtagttag
     1621 aaatcttttc gcgaagattt gatttcgtca ttgatacctc caagagttat tctcataccg
     1681 tttctcagtc tctcggcttc atggtcggga acaacaacac atcacgaatg gagtagctat
     1741 cggtcagcag catagccaga cggtcgatac caatgccgat tcctcccgtt gggggcatac
     1801 catactccaa agcggtgatg aagtcttcat ccatcatacc agcctcctgg tctcccgctt
     1861 cccgcagctg aacctgcctt aaaaaacgtt ctttctgatc aatgggatcg ttcagctcgg
     1921 aaaaagcgtt gcccatctcc cggcgggtaa taaagaactc aaaccgttcg gtaagctcag
     1981 gcatgtcctg ctttcttttg gagagcggag atacttcaat cggataatcg agaatgaacg
     2041 tcggttcgtt caggttctct tctacttttt catcaaaaat catgtaaagc gcgtcgcccc
     2101 aggtcttgtc ttcgccgcat tcaatcccca tgtccttcgc gacagcccgc gctttttcgt
     2161 gatcgcccct gaaactcata aaatcggcgc cggtgtaggt tttaacggct tcagccatgg
     2221 tcattcttct ccagggggga gtcagatcga cttcatcgcc ctggtaagtg atcacggttg
     2281 aaccggtaac ttcttttgca acgtgggcaa tcatatcttc ggccagtttc atcatgcctt
     2341 tataatccgt ataagcctgg taaatctcaa tggtcgtaaa ttccgggtta tgcttgattg
     2401 acattccttc gttgcggaat atccggccca tttcgtagac ccgttccatt ccgccgacaa
     2461 tcaggcgttt gagataaagc tccgtcgcga ttctgagata aaggtcgatg tccagcgtat
     2521 tgtgatgggt gatgaagggt cttgcagttg ctccgcccgg gataacattt aaaatcggcg
     2581 tttccacttc aataaagttc tgagcatcca gaaaactccg gatacagctg atgatcctgc
     2641 tgcgtttgat aaatgtgtcc ttcacttccg gattgacaat caaatccaca taacgctgac
     2701 ggtagcgcaa ctcggtatct ttcagaccat gccatttctc cggcagcgga agaagcgatt
     2761 ttgataacag cgttatttca ttggcatgaa tcgaaatttc tcctctgcgc gtgcggaaca
     2821 caattcccct gatgccgacg atatcgccga tatcccactt tttaaactcc gcgtagggct
     2881 cttcacccac gtcgttgatt ctcacataga cctgcattct accggttgcg tcgtggacat
     2941 caataaaatt tgctttgccc atatctctcc ggctcatcat acggcccgcg atggagacct
     3001 ccttgccgtc aagttcatcg aaatgctcgg caatctcgct gtttcgttta tcacggtcat
     3061 aggttgttat cccaaagggg tctttattat ttgcctgcag ttcttttaat ttttctcttc
     3121 tgattctgag tatctcgtta aattcctcct gggtctggtt gacattgttt tcaatttctt
     3181 cagacattta taattcccct tgtaattttt tatgaattta ggcagctgta tttattttat
     3241 tatatctttt gcctttttgc aaatgaatgc cgggaaaaat gaagaaaagc gggcattctg
     3301 cccgcttttg accctttctg tttgatttca gcctattttg aaatcatttg aattttaaac
     3361 tgaaccacac cgccgggagt ttcgacatcc accacatcgc caaccctatg gctaagaagt
     3421 gaacgcccga cgggagactc atcggaaatt cggttaatat caggatcggc ttcggttgag
     3481 ccgacgatct gataagagat ttcctcgtta aattcaaggt caagaacggt aactttagaa
     3541 ccaagattta cgatttcggt atttatttca tcatcgtcga tcagtttcac atttttgagc
     3601 atcgcctcaa tctgcgcgat ccggatttca accgtggcct gttcattttt ggcttcatcg
     3661 tattcactgt tttccgataa atcgccaaaa gaaagcgcaa cttttatctt ttctgcgatt
     3721 tctttccgtt tctccgtttt gagctgttcc agttcatctt ctaattttct taagccctcg
     3781 tcagttaata tgacctgttt agccatattt gttctctcct tttaaggcgg cggaagccgc
     3841 tcaaaattta ataatatgca taaatatcgc aacggagcct tccttgttgc cgattggctt
     3901 gcactagata taatacgtat tatagtaaac cagctgttaa ctgtcaagtc gtgaaagccc
     3961 cgacgtaatc aaatcgcaat caatcgggtc ccctctggaa agacaccgga cgagcggcag
     4021 actgtcgagc ggcgcaagtt ccgcgtgctg ccacagtgcg gctgcaaacg ccagcttatc
     4081 aatttccggc ggccgaatac tttcaagaat ctcaggaatc tccggctcga atccgtctat
     4141 tacagggccc tcccagcttt cagggacggg cttagcactg acggtaagcc ccgggagccc
     4201 atgcgggtcg aagccttcca caccataagg cgccagaata atgtggcacc ttgccagtcc
     4261 atcttgattc tgagtgtatt tcagactgtc tttccgatca tcggccagca aagaccgaaa
     4321 aaattcatac ttccattctt tctgtgtata aattgtcacg ctccgaaaaa ccggtattgc
     4381 ttctgcggca agatgcccat tgctgtaacc gttagtatca agaatggccg tatccagctc
     4441 ggacagaacc agacccgcat ccttcgccgc ctttaccgac atgcgcagac aataattcct
     4501 cagatattgt tccggagaac aaatcattgg cttaaccgct tccgggcagg gcataccggc
     4561 cggaagcaga tatttgttga ttccattttt cctggcaaaa gagagaatcc gattccaacg
     4621 gggctggccg cggctgtcaa gagaactctc gatatccaga atgctgatca cgccgttgct
     4681 gatttcaaaa agtctgggtt ttggagccag aaagcgctcc agaaaaccga atctttggta
     4741 atcatgacag cagggcaaaa acgttactgc ggcaaacata cattggtacc tccaaaactc
     4801 ggggtcagcc tttcgattgg ctaccaatat atatgccgca gaaatttgaa ctatgtcctt
     4861 gcccggcgtc cgaatttcag gcagattctt aggacccggc gcagggggct gcgggaatgc
     4921 atttattgat ttttcccatc ttccgcatca atataccgag accgtttttg cagcgttgcg
     4981 gttctgctat tgaatcaaag ttttgacata attcagcccc gcctggagct ggtcgtcttc
     5041 ctgatcgctc agctgataaa atcggctgag tttggcactg ctgagatcca cctcgatatc
     5101 cggagtaacg ccttttcctt cgaaattatt actctttggc ggatagaaat aagcgatgga
     5161 aaggtcgagc gcagttccat cattcagatc gaagatggtt tgcatggttc cttttccata
     5221 agttttggtt ccgattgatt tggcctttcc ataatccttc agcgcagccg taaacaattc
     5281 tgcggcgctg gctgtattct gattggtcaa aaccgccatc ggcagactga tttgagccgc
     5341 gtctgaacgg taaagcgtgc tgattgaccc ggttttatat tttgcccgga caaccggccc
     5401 cgcgggaaca agcttatcga tcatcttttc aaccgtatcc agcagtcctc cgggattatt
     5461 ccttacgtcg aacacaatcc ctttcgcgcc ctttttcacc agattatcca cctgggcgga
     5521 gaactgttca tccgtgttgg aatcaaactc caatatccgg acataagcga gattcccgat
     5581 cattctgctt tccaccgact gactttcgaa ctgtttccga acaatcgaga aaggtttttc
     5641 aactccattg cgcttcactg taaattccgc cttggttccg gcatcgccct taagaaggtt
     5701 tacggcgctt gcgtaaccca gcgccttgac gtcttcactg ccgattttga caatcacatc
     5761 accggcgaga actccggccg cctcggcagg ggactttttc acgaccgaca ctacgttaat
     5821 atatccttcg gcattttcaa taacattgac accgattccg attccttttc cctgaatatc
     5881 ggtttcaatc tttttataat cttccgcgtc aagataagtt ccgtatttat cagagatgcc
     5941 cttcatgtat ccgttgatca attcatcttc cagtttggtt tcgtcgattt ttccgacgta
     6001 cctttccctc acctttgcgt caacatccgc aagcttttta aacatggact cccttgcaga
     6061 aaaatcagtg aggttcttat tgatcatctt catggaaaat atcatggtta tggtgacggc
     6121 aacggccgct gtcatgagca taagcataac agccgctccg acgctgatct ttttgttcat
     6181 tttaagcccc atttcgtcgc ttcgcaccgg cgtagaataa gaaaagcaac aatttaatgc
     6241 agataattca aagggttttg cgccttgccg tttacataca caccaaaatg cagatgggcg
     6301 gccgttgact tgcccttaga gccgacatag gcgatcacct ggccacgctg gacacttgcc
     6361 ccctctttta ttccggaagc tctcgcagag cagtggccat agaaggatac gatgccgtta
     6421 tgatggttga taaacacata attgtcgccg taataataca caatatcttt agctgccagt
     6481 ttcgcgaatt tctgtaatac gattgttccg gagtccgtag cataaatcgg gctgcccatc
     6541 gacgcggcaa tatcaattcc gtcatggaaa ccccatattc ctgttatcgg atgtgttctc
     6601 caaccgtaag aatccgtaat tcttcgtact gtagtcggcc ag
//
LOCUS       DATALY010000207         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_733464,
            whole genome shotgun sequence.
ACCESSION   DATALY010000207 DATALY010000000
VERSION     DATALY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733464"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1034)
                     /locus_tag="VHR42_06045"
     CDS             complement(240..1034)
                     /locus_tag="VHR42_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3026771.1"
                     /translation="MTIDFHTHIFPDDLAPKALSALLSNINGIYHPVTNGTKDSLLKN
                     MNQWGIDISVVQPVITKLSQTAKINQWAKGICSDRLISFGGIYPHTDDYKRDIDFVVD
                     LGLKGLKFHAEYQNFNIDDPQMLKIYDYALSRGLIILHHAGFDPGYPAPYKSSPKQFA
                     KIADAMQGGVMIAAHFGGHAQWDDVEKYLIGKNIYLDTSMGFEYFPHEQFLRIVKAHG
                     ADKILFASDSPWSNAKAEIETIKSLQLNEQEKEMILGLNAKRILGI"
     gene            complement(1072..2085)
                     /locus_tag="VHR42_06050"
     CDS             complement(1072..2085)
                     /locus_tag="VHR42_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX3026772.1"
                     /translation="MKAAFVKSPFQFEIREVRLREITDDEVLVKVKACGICGSDTHTA
                     ETLADDWRPFGHEIAGIVEKTGKNIRNVKPGQSVVLESGSFCRECTLCRNGRVDLCND
                     APNIFSYQTMGFAEYIIAPKECVVPFDGLSFAEASLTEPLGVALDLFYSTDIQLNDDV
                     LVIGLGPIGLMALRLAKLCGARNVYGAARSYSEARIELAKKFGASDVILTDKQDIKDF
                     EFPRGGVEKIMITASPATIPDSFEAALNGGIISFLGEDFGEGADISFNANYFHWKKLQ
                     LRSSYAAPALYFPRCIELIKSGAVDAKALISHTFGLKEIAGAMETVKNDKKHVLKSVM
                     LQD"
     gene            complement(2158..>2397)
                     /locus_tag="VHR42_06055"
     CDS             complement(2158..>2397)
                     /locus_tag="VHR42_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX3026773.1"
                     /translation="RYVQDQYSFFNLFDGGVFSCDIHMLKPSPEIYRYFLDQYRLDPH
                     DCLFFDDMEENIKAAENFGIKGILFTGEQKVKDFI"
BASE COUNT          616 a          579 c          521 g          681 t
ORIGIN      
        1 ttctcagggc gcgttggccg gcaggggaat gattgagttt gtccgcgcaa aagcgaaata
       61 gaggaatcaa tcaatcggtt tggcttcgcc gggtgtgact gttgtcgccc ggcggagctt
      121 tttgaaaggc tttttgccgg ctatttccgg gatcgagaat tgttttacgt atctccccgc
      181 cgaaggcggg gagatacgta gcttatccct cttcaaaaca ccgggtcacc gtaatcattc
      241 taaatcccca gaattctttt agcgtttaaa cctaaaatca tttctttttc ctgttcgtta
      301 agctggagtg attttatggt ttctatttca gctttcgcat tactccaggg ggaatctgat
      361 gcaaacagaa ttttatccgc cccatgtgct ttaacaatcc gaaggaattg ctcatgagga
      421 aaatattcaa accccatgga tgtatcaaga tagatgtttt tcccgatcaa atatttttcg
      481 acatcatccc attgagcgtg tccgccaaaa tgagccgcga tcataacgcc gccctgcata
      541 gcatcggcta tttttgcaaa ttgcttgggg ctgctcttat aaggagcggg atatccgggg
      601 tcaaatcctg catgatgtaa aataatcagt cctctgctta gcgcgtaatc ataaatctta
      661 agcatttgtg gatcgtcaat attaaagttc tgatattccg catggaattt cagccctttc
      721 agccctaaat ccacgacaaa atcaatatct cttttatagt cgtcggtgtg cggataaatt
      781 ccgccgaaag aaataagccg gtcggaacaa atcccctttg cccactgatt aatttttgcc
      841 gtttgagaca gtttggtgat gaccggctga actaccgaga tatcgatgcc ccattgattc
      901 atatttttca gcaaactgtc ttttgttccg tttgtaacgg gatgatagat gccgttgatg
      961 tttgataaga gggccgacaa agcctttggg gccaaatcgt ccgggaaaat atgcgtatga
     1021 aaatctattg tcatgaagtc acatcctgag tatgaaatat gatctgcttt tctaatcctg
     1081 aagcatgact gattttagaa catgtttctt gtcatttttg actgtttcca tagcgcccgc
     1141 gatttccttt aatccaaagg tatgcgaaat cagcgccttg gcgtcaacgg cgccggattt
     1201 gatcagctcg atgcacctcg gaaaatataa agccggcgcc gcgtaggaag atcgcagctg
     1261 aagctttttc cagtggaaat aattcgcgtt gaagctgata tcagcacctt cgccgaaatc
     1321 ttcccctaaa aaagagatga ttcctccgtt taaggccgcc tcaaaagaat ccggaatcgt
     1381 ggccggcgac gccgtaatca tgatcttttc gaccccgccc ctgggaaatt cgaaatcctt
     1441 gatatcctgt ttgtcggtca aaataacatc ggacgctccg aactttttgg ccagttctat
     1501 ccttgcctct gagtaggaac gggcggcccc gtagacgttt ctcgctccgc agagtttcgc
     1561 cagccgcagc gccatcagac cgatcgggcc aagaccgatt accaggacgt catcgttgag
     1621 ctggatatcg gttgaataga aaagatccag cgccacgccc aaaggctccg tcagagaggc
     1681 ttccgcgaaa gacagcccgt cgaaggggac gacgcattct ttcggggcaa tgatatattc
     1741 ggcaaaaccc attgtttgat aggaaaatat attgggcgcg tcgttgcaaa ggtccactct
     1801 cccgtttctg cacagcgtgc attcccggca gaacgatccg ctttccaata caaccgattg
     1861 gcccggcttg acatttcgga tattctttcc cgtcttttcc acaattcccg cgatttcgtg
     1921 cccgaaaggc ctccagtcgt ctgccagagt ttccgccgta tgggtatccg agccgcagat
     1981 tccgcacgcc ttgaccttga ctaaaacctc atcgtctgta atttctctga gccgtacctc
     2041 cctgatctcg aattgaaagg gtgattttac aaaagcagcc ttcatttttt tactcctttt
     2101 tcaatcgtaa atggatggtt gatatgcttt tttacggatt atgcccatac catggcttta
     2161 aataaaatct ttaacctttt gctcgccggt aaataaaatt cctttaatcc caaaattctc
     2221 cgccgccttg atattttcct ccatgtcatc aaaaaaaaga caatcatgcg ggtcgagtct
     2281 gtattgatct aaaaaatacc gatagatttc aggggaaggt tttaacatat gaatatcaca
     2341 ggaaaatact cctccgtcaa ataaattgaa aaaagaatat tgatcctgca catagcg
//
LOCUS       DATALY010000208         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_733493,
            whole genome shotgun sequence.
ACCESSION   DATALY010000208 DATALY010000000
VERSION     DATALY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733493"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VHR42_06060"
     CDS             complement(<1..766)
                     /locus_tag="VHR42_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HEX3026774.1"
                     /translation="MKVLFLSNTAGQGHHSTANALMEMLSAEGAECKMLDTYAYINPV
                     LYEAIARGYLLTTSITPKAYGGVYRILEKKDKNDSKYSVINVANSIMSLKLRKYFEKE
                     FNPDVIVCTHIFSAQLVNVMKASGLIKQKSIGIITDYTIHPFWQDVGHIDYFVTASEL
                     ITSQAVKRGIPAEKILPFGIPIHHKFSKKVDKTEARRQLGIDPDLFTILVMSGSMGYG
                     NLSEVLQKMDSLGTDFQILAVCGNNKHAKTKIDRLKT"
     gene            complement(931..1218)
                     /gene="spoVG"
                     /locus_tag="VHR42_06065"
     CDS             complement(931..1218)
                     /gene="spoVG"
                     /locus_tag="VHR42_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX3026775.1"
                     /translation="MNITDIKIRKIFTEGKVRAVVSIILDGDFAVHDLKVIEGVERLF
                     VAMPNRRSEDGRFQDIVHPITSEARTKLETEILDKFNEASKNLHEEQDGAQ"
     gene            1384..>2516
                     /locus_tag="VHR42_06070"
     CDS             1384..>2516
                     /locus_tag="VHR42_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX3026776.1"
                     /translation="MFVYIGFMLLFKNFKHGPSFLKNQIAVILHFVKINKLSRGVYRV
                     ISDPEILNKVKYLHFVGIGGSGMSGLAEILHSRGYQITGSDNNESDTLSHIKSLGIPV
                     AMGHRAENIGSAQLVIHTAAVHGDNPELMEAKKRGIPVIDRSVLLGLISLKYPDTIAV
                     SGTHGKTTTTCMLTQILLTAGLDPTAVIGGKLPLIGSNSRIGDTDLMVCEACEFVDSF
                     LNLRPAVSIILNIDADHLDYFKTVDNLISHFKTFTTLTSRALIYNGDDANTLAAVREY
                     PLRKITFGFGKENDYSARVRNDRKGLMSFDILQKGETFATVSLKVPGKHNILNALAAC
                     AAAVEAGADVKAIERGLNEYAGAGRRFEVLGVINGVTIADDYA"
BASE COUNT          667 a          609 c          549 g          691 t
ORIGIN      
        1 gtgttttaag ccggtcgatt ttcgttttcg cgtgtttgtt gttgccgcag acagcaagaa
       61 tctgaaagtc ggttccgagc gaatccatct tttgcagaac ttctgacaaa tttccatagc
      121 ccatgcttcc gctcatcacc agaatcgtaa agagatccgg atcgatgcca agctgtctgc
      181 gtgcttcggt cttgtcaacc tttttgctga atttatgatg aataggaatt ccgaacggga
      241 gaattttctc agccggaatc ccccgcttta ccgcctgact ggtaatcagt tcgctcgctg
      301 tgacaaaata atcaatatgt cccacgtcct gccagaacgg atggatggtg taatccgtaa
      361 taatcccgat gcttttctgc ttgatcaaac cgctggcttt cattacgttg accagctgag
      421 cggaaaaaat atgggtgcag acaatcacat ccgggttaaa ctctttttca aaatactttc
      481 ggagcttgag cgacataata gaattcgcaa cattgattac ggaatatttg gaatcgtttt
      541 tatctttttt ttctaagatc cggtagactc ccccataagc cttgggggta atcgacgtcg
      601 taagaaggta tccgcgggca attgcttcat aaagcacagg attgatatag gcataagtat
      661 caagcatctt gcattcggcg ccttccgccg acagcatctc catcagcgcg ttggcggttg
      721 aatgatgccc ctgaccggca gtatttgaca aaaacaatac tttcatggcc tgctcctctt
      781 cctccgtttg aaacttcgat gctcgggggg tcggccggcg tatggaatta cagactccat
      841 ccgcaaaccc tgcatttgcc aattcagtat atcacacata aaagagtgtt acaacataaa
      901 ataaagcggc gggaaagtcc tccgccgccc ctactgcgca ccgtcctgct cttcgtgcag
      961 attcttggac gcctcattga atttatccag tatctcagtt tccagctttg ttcttgcctc
     1021 cgatgtaatc gggtggacaa tatcctgaaa tctcccatct tccgagcgtc ggttcggcat
     1081 ggccacaaac agccgctcca cgccctcgat tacttttaaa tcatgcacgg caaaatcgcc
     1141 gtcaagaatg atggaaacta cagcccgcac ttttccttct gtaaaaattt ttcttatttt
     1201 gatatcagtg atattcattt atgaatctcc tttatcttaa tcactacaat aaatcccttg
     1261 atttttcttc tgtttcttac cagtattatt cctcttgaca aacgtattta tgttgtaact
     1321 ttcattacat tgtggtttta attacgaaaa aaggtttatt ttaagccgaa taggacgtat
     1381 aatatgtttg tttatatcgg ctttatgctg ttatttaaaa attttaaaca cggcccttct
     1441 tttctaaaaa atcagattgc cgtaattctg cattttgtca aaattaataa actgtcgaga
     1501 ggtgtttaca gagtgatcag cgatcctgaa attctcaata aggtaaaata tttacatttc
     1561 gtaggaatag gcggctccgg tatgagcggc cttgcagaaa ttctgcatag caggggttat
     1621 caaattaccg gctccgacaa taacgaatcg gataccctga gtcatattaa atcacttgga
     1681 attcccgtag caatgggcca cagagccgaa aatatcggca gcgcgcagct ggtaattcac
     1741 accgcagcgg tacacggtga caatccggaa cttatggaag ccaaaaaaag gggcattccc
     1801 gttattgacc gctcggtgct tctggggtta atcagcctga aatatccgga cacgatagcc
     1861 gtttcaggta ctcacggaaa aacgacaacc acctgcatgc tgacgcagat tttgctcaca
     1921 gccgggctcg atccgaccgc cgtcatcgga ggaaagcttc cgttgatcgg ctcgaacagc
     1981 cggatcggcg acacggatct gatggtttgc gaagcctgcg aatttgtcga ttccttcctc
     2041 aacctgcgcc cggccgtatc catcatcctg aacatcgacg cagatcatct ggattatttt
     2101 aaaacggttg acaatctgat cagccatttt aaaaccttta ctactctgac atccagagcc
     2161 ctcatctaca acggcgacga tgcgaatacg ctcgcggcgg tccgggaata cccgctcaga
     2221 aaaataacct tcggcttcgg taaagaaaac gattacagcg cccgtgtaag gaatgaccgc
     2281 aaaggcctga tgagttttga cattttacaa aaaggtgaga catttgccac cgtcagcctg
     2341 aaagttcccg gaaaacataa tattctgaac gcgctggctg cctgtgccgc agcggttgaa
     2401 gcgggcgccg acgtaaaagc aattgaaaga ggcctgaacg aatatgcggg agccgggaga
     2461 cgatttgaag ttctcggggt aattaacggc gtcacgattg cagacgatta cgctca
//
LOCUS       DATALY010000209         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_389846,
            whole genome shotgun sequence.
ACCESSION   DATALY010000209 DATALY010000000
VERSION     DATALY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389846"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..1986
                     /locus_tag="VHR42_06075"
     CDS             592..1986
                     /locus_tag="VHR42_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX3026777.1"
                     /translation="MRKEHDLLGELCVAGEKYYGIHTQRALSNFNITGVPIHRELVLA
                     MVVIKKAAAQTNRLAGLLEDKKADAITAACDEILAGSLAEQFVVDSLQGGAGTSANMN
                     VNEVIANRAIEILGGERGDYSLIHPIDHVNLSQSTNDVFPTAVRIAAIRMLKPVSEAF
                     AALQTALQEKEEQFSAVLKVGRTELQDAVPVTLGQEFGAWAQAVARDRWRLYKVEERL
                     RQVNIGGTAVGTGLNASKKYIFNMIEKLRELTGIGLSRAEYMMDPTQNCDVFVEVSGL
                     LKAAAVNLAKISSDLRLLSSGPRAGIGEIVLPAVQSGSSIMPGKINPVIAEAVNQAAY
                     QVMADDFAITLAAQAGQLELNAFLPLIAKNLFEMLDLLNNVVRLFIDRCVKDIRADEC
                     KCRELVENSLVLATALTGALGYDKASQIAAECAETGKTIRQALEEGELLDKAQIERIL
                     NTEAMTKPGFPGKK"
     gene            2011..>3047
                     /gene="hydF"
                     /locus_tag="VHR42_06080"
     CDS             2011..>3047
                     /gene="hydF"
                     /locus_tag="VHR42_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HEX3026778.1"
                     /translation="MDSTPMANRISISFFGKRNAGKSSLINALTGQKVAVTSDVAGTT
                     TDPVYKTMELLPLGPVVFIDTPGLDDEGELGSLRVEKTYEVLRKTNLAILVAGAREGI
                     TDFEKDFARELKGRKIPCVGVINKSDLAAVPPEKLAEYGNALGIPMVSASAETGDGIG
                     RIKEMIIQNAKFDDAELGLVGDLIQPGDIAVLVTPIDKAAPKGRLILPQQQVIRDIIE
                     CDATAMVTKEHELRQTLDSLKRKPAIVITDSQAFLKVSADTPQEIPLTSFSILFARQK
                     GDLAQMVQGIRKIENLKPGGRVLIAEGCTHHRQSDDIGKVKIPRWIRQIVGGDIQFEW
                     ASGACFPRDVDR"
BASE COUNT          738 a          714 c          867 g          728 t
ORIGIN      
        1 cacgccatat gttggaagtg ccgtagtccc aaaccataaa tctgcatttg agtcccaata
       61 tgttttattg gtttttagta tttcatcttt tttagctctc gaatagcagt ataacttctc
      121 actttacaaa caatcaacat cggattaata ataccattat taaccacaga ttacaataaa
      181 accgccctcg tagatagagc ggtttttctg ccttaaaaag tatcttattg cccgtcctct
      241 caaataaggc gagttttagc tattcaggta attaagcaat tgatcggccg ctgataaaaa
      301 accatattct tgcgccatag cgttttttgg agtgattgct tgagcaacgg tatatttccc
      361 attggaagtt ttatttaacg ggaaatatta agctactgtt ttttctttta aattgtattt
      421 cctcgcggca ggaggtataa taaagaaaaa tatcattctt tttattggag cctgagcctc
      481 ggctcggcgg tcggtttcta tagccgcaga agcggttatt tctttatgat ataactatac
      541 tgaaaaggtc gatattgaat cgccggttac ggtgtcggga ggcggagcgt cttgagaaaa
      601 gagcatgatt tgctcggtga actgtgtgtc gccggagaaa aatattacgg gattcatacg
      661 cagagggccc tttccaattt taatattacc ggtgttccca tccatcggga gcttgttttg
      721 gccatggtcg tgatcaaaaa agcggcggcg cagaccaacc ggctggccgg tctgctggag
      781 gataaaaagg cggatgcgat aacggcggcc tgcgatgaga tcctggccgg gagtcttgcc
      841 gagcagtttg tcgtcgacag tcttcagggc ggcgcgggca cttcggccaa tatgaatgtc
      901 aatgaagtga ttgcgaaccg ggcaattgaa attcttggcg gggagcgggg cgattattcg
      961 ctcattcacc cgatcgacca tgtgaatctg tcacagtcga cgaatgacgt ttttccaacc
     1021 gcggtgcgaa tcgccgcgat tcggatgttg aagcctgtca gcgaggcctt tgcggcgctc
     1081 cagaccgcgc tgcaggagaa agaggagcaa ttctcggccg tgttgaaagt gggtagaacg
     1141 gagctgcagg atgccgttcc ggtcacactg ggccaggaat tcggagcgtg ggcgcaggcc
     1201 gtggcgaggg atcgctggag gctttataag gtcgaggagc ggctgcgtca ggtcaacatc
     1261 ggcggcactg ccgtgggcac cggtctgaat gcctcaaaga agtatatctt caatatgatt
     1321 gagaaacttc gggaattgac gggaatcggc ctgtcaaggg cagagtacat gatggatccg
     1381 acccagaact gcgacgtgtt tgttgaggtt tcgggattgt taaaggctgc cgcggtcaat
     1441 cttgcaaaaa tctcttccga tttaaggctt ctctcctccg ggccgcgggc ggggatcggt
     1501 gaaattgtcc tgccggccgt ccagtcggga tcttccatta tgccgggaaa aattaacccg
     1561 gttattgcgg aagccgtcaa tcaggcggcc tatcaggtga tggccgatga ttttgccatt
     1621 acgctggcgg cccaggccgg gcagctggag ctgaacgcct ttttgccgct gattgccaaa
     1681 aacctttttg aaatgctcga tcttttgaat aatgtcgtcc gtctgtttat cgaccgatgc
     1741 gtcaaagaca tccgtgcgga tgagtgtaaa tgccgtgaac ttgtggaaaa cagtctggtt
     1801 ttggcgacgg cgctgaccgg cgcgctcggt tacgataaag cctctcaaat tgcggcggaa
     1861 tgtgccgaaa caggcaaaac gatccgtcag gcgcttgagg agggcgaatt gctggacaag
     1921 gctcagattg agcggattct caataccgaa gcaatgacca aaccgggatt tcccggtaaa
     1981 aaataacgat tgcggattat ggaggtaaca atggacagca caccaatggc aaaccggatc
     2041 agcatctctt ttttcggaaa acggaacgcc ggaaagtcct ctctgattaa cgctctgaca
     2101 ggccagaagg tggcggtgac gtccgacgtg gcgggaacga cgacagaccc ggtctataag
     2161 acgatggagc ttctcccgct tgggccggtg gtctttatcg acacgccggg tcttgacgat
     2221 gaaggcgaac tcggcagcct cagggtggaa aaaacctatg aagtgctccg caagaccaat
     2281 ctggcgattc tcgtcgcagg cgcccgggaa ggcattaccg attttgaaaa ggattttgcc
     2341 cgcgaactga aaggccggaa aattccctgt gtgggtgtga tcaacaaaag cgaccttgcc
     2401 gcagtgccgc cggaaaagct tgcggaatac ggtaacgctc ttggtattcc gatggtttcg
     2461 gccagcgccg aaacaggcga tgggatcggc cggatcaaag agatgatcat tcaaaacgca
     2521 aaattcgacg acgccgagct cgggttggtg ggtgatctga ttcagccggg cgacatcgcc
     2581 gttcttgtga cgccgattga taaggccgcg ccgaagggca gactgattct gccacagcaa
     2641 caggtgatcc gggatattat cgagtgcgac gcgaccgcga tggtcacgaa agagcacgag
     2701 ctccggcaaa cccttgacag cctcaaacgg aagcctgcca ttgtcatcac cgactctcag
     2761 gcgtttttaa aggtctcggc ggatacgccg caggagattc cactgacctc tttttccatc
     2821 ctttttgcac ggcagaaagg cgatctggcc cagatggtgc agggtatcag gaaaatcgag
     2881 aatttgaaac ccggcggcag ggttttgatc gccgaaggct gcacccatca ccggcagtcg
     2941 gatgatatcg gaaaggtgaa gattccgaga tggattcgcc agatcgtggg cggagacatt
     3001 cagtttgaat gggcctcggg ggcttgtttt ccccgggacg tcgaccg
//
LOCUS       DATALY010000210         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_220311,
            whole genome shotgun sequence.
ACCESSION   DATALY010000210 DATALY010000000
VERSION     DATALY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220311"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1661
                     /locus_tag="VHR42_06085"
     CDS             360..1661
                     /locus_tag="VHR42_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012193499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX3026779.1"
                     /translation="MIWDKAAECSDKKQMRALQLEKLQYIVRYAYENVSFYHEKFDKI
                     GLRPEHFKTLKDIEKIPFTTKNDLRDNYPFNLFAKPLGKIVRLHASSGTTGKPVVIGY
                     TKKDMNIWTNCVSRMAAAAGVTEDDIAQIVFGYGMFTGGFGLHYGLENMGVTVIPSSA
                     GNSERQLMFLQDFHPTVLVGTPSYVLHLSELAQEHGLSPKDFSLRVGLFGGEGHTAEM
                     RREIEKRWNIDATENYGLTEITGPGVAGECLEHCGMHINEDHFYPEIIDSKTGATKPI
                     GEKGELVLTTLSKEGVPMLRYRTKDITRLLEGPCKCGRTNIRMEKVLGRTDDMLIIRG
                     VNVFPSQIESVLVGMEHISPHYQIIVTKNGFMDAIEVHVELSDGSLLNRYSELENLET
                     EIRHKIKSALLIDSKVKIVEPKSIERSLGKAKRVVDLRGQK"
BASE COUNT          499 a          441 c          439 g          436 t
ORIGIN      
        1 agactatttt tgccgggaat cagggatgtt attcaaattc tgtaagaatt tcttataaac
       61 ttcaaatatc cccgcgattt ccttccacct gtagcaaaag gttgaaataa gacagaaaat
      121 gtgccataat ttatctgtaa ttattcgttc cttttcacaa ttttccaccc attgaattca
      181 gcgccgtgat atgatatgat tatagtgcaa ttttatctga taccgctttg cattgtaaaa
      241 gcgacgacgg aaacagaata aaccgctgtt tcattttgag cggcacagtt ttccccatgt
      301 gtccaatgct gtgacagctc cgcgaaacag acggtttttt taaacaggag atggatcgaa
      361 tgatctggga caaggcagcg gaatgttccg acaagaaaca gatgcgcgct cttcagcttg
      421 aaaagcttca gtacatcgtg cggtacgcct atgaaaatgt gtcgttttac catgaaaaat
      481 tcgataagat cggccttcgg cccgaacact tcaaaacact gaaggacatt gaaaaaatcc
      541 cttttaccac caaaaacgac ctgagggaca attatccttt taacctgttt gcaaagcccc
      601 ttgggaaaat cgtccgtttg catgcgtctt ccggaacgac cggaaagcct gtcgtaatcg
      661 gttatactaa gaaagatatg aatatctgga caaactgcgt ttcccgtatg gcggcggcag
      721 caggcgtaac ggaagatgat attgcccaga tcgtgttcgg atacggaatg ttcacgggag
      781 gatttggact tcattacgga ttggaaaaca tgggcgtcac cgtaatccct tcttccgccg
      841 gaaattcaga acgccagttg atgtttttac aggacttcca ccccaccgtt ctggttggca
      901 cgccctccta tgtgcttcac ctttccgagc ttgcacagga acacggcctg tccccaaagg
      961 atttttccct tcgcgtcgga ctgtttggag gggagggcca tacggctgaa atgcgccggg
     1021 aaattgaaaa gcgctggaat attgacgcga ccgagaacta cgggctgacg gaaatcaccg
     1081 gtcccggcgt cgcgggagaa tgcctggagc actgcggcat gcacatcaat gaagaccact
     1141 tttaccctga aatcatcgat tcaaagaccg gagcaacgaa accgatcggc gaaaagggag
     1201 aacttgtcct cacgacgctg tctaaagaag gcgtccccat gctgcgctac cgcacgaaag
     1261 atatcacccg tctgctggaa ggcccttgca aatgcggcag gacgaatatc cggatggaga
     1321 aagtcctcgg acggacggac gatatgctga ttatccgcgg cgtgaacgta ttcccgtccc
     1381 aaattgaaag cgttctggtc ggcatggagc acatcagccc gcattaccag atcatcgtca
     1441 ctaagaacgg atttatggac gcaatcgaag tacacgtcga gctgtcggac ggttccctgc
     1501 tcaaccggta cagtgaactt gaaaatctgg aaaccgagat ccggcacaaa atcaaatcgg
     1561 ccctgctgat tgattccaag gtgaaaatcg tcgaaccaaa atccattgag cgctcgctcg
     1621 gcaaggcaaa acgggtcgtc gaccttcggg gccaaaagtg atattacaac ttaaataatg
     1681 tttagtttac ataaaccggg aggtatcttt ttgaaacgtt tgtttttagg caacgaagcc
     1741 gttgcaaccg gcgtgcatga cgcgggagcg accgtcgttt ccgcttatcc gggtacgccg
     1801 agcactgaaa ttacc
//
LOCUS       DATALY010000211         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_650575,
            whole genome shotgun sequence.
ACCESSION   DATALY010000211 DATALY010000000
VERSION     DATALY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650575"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VHR42_06090"
     CDS             complement(<1..199)
                     /locus_tag="VHR42_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026780.1"
                     /translation="MQSRQTTFNLFSGILALMISLAVNFFLSPFIVRHLGEEANGFMQ
                     LANNFVTYASLLTLAFNSMASR"
     gene            complement(174..1184)
                     /locus_tag="VHR42_06095"
     CDS             complement(174..1184)
                     /locus_tag="VHR42_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX3026781.1"
                     /translation="MKKFKRKHGRIVIFIATPTHGNLGDHAIVYAQHRFMEQIGVGDR
                     VFELTRQQYEFCREQLKTVITPDDVLIIDGGGNIGTLWIEEENKMRDIILRFPKNPIF
                     VFPQTAYYSNDIFGRAELEKSAKIYCSHRKLTLFARDSDTDRLLKREFRSIPTFFVPD
                     IVLFITDALKNATCRAGVLLCMRGDTEKITAEESLGNIEKYLIQCHYDYRKISTVVDH
                     TVTARMRERELFRKWAEFSSARLVITDRLHGMLFAAITGTPCLALDNVSHKVKQGYEW
                     IGYLPYIKFCENPDELICNITEFYEMGEQFYRAEPLLAYFSQMQSKVKRTVDNAEQTN
                     NL"
     gene            complement(1271..2278)
                     /locus_tag="VHR42_06100"
     CDS             complement(1271..2278)
                     /locus_tag="VHR42_06100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026782.1"
                     /translation="MQPLISVVVPVYGVEKYLKKCVDSILHQTYQNLEILLVDDGSPD
                     YCPLICDRYAKEDPRVRVIHKANGGLSSARNAGIEASSGEYIGFIDGDDSVEPDMLEQ
                     LERSCSESGAEIAVCDYFLERSDGSQIFPVDHVPDGILTGDQALDQFLRNGFVYLVIA
                     WNKLYARKLFFNNDIRYPVGRFFEDNCTTNRLLYKSERVALIGRPLYHYLQRDGSIMA
                     QPVDAKKIRDRIAVADSYLQFAKETGSHFHQAQSAALLMLLYQYGEFYSLRSLVPELR
                     KKILQLASGWRKNPYLSKRFPFQLILVRFYLFPYVRHIWMALFRPDTERRTLPPVQVG
                     K"
BASE COUNT          642 a          618 c          540 g          674 t
ORIGIN      
        1 aacgactggc catcgagtta aaggcaagtg tgagtaaaga agcgtacgta acgaaattat
       61 tagccaattg cataaatccg tttgcctctt cgccaagatg cctgacgata aaaggagaaa
      121 gaaaaaagtt cacggccaaa ctgatcatta aagctaaaat tccggaaaat aaattaaagg
      181 ttgtttgtct gctctgcatt atcaaccgtc cttttcactt tgctctgcat ttgagaaaaa
      241 tatgcgagca ggggctccgc acggtagaac tgttcgccca tttcatagaa ttctgtgata
      301 ttgcaaatta gctcatctgg attttcgcag aattttatat agggcaaata tccgatccac
      361 tcatatcctt gttttacttt atggctgaca ttatccagag ccagacacgg tgtgcctgta
      421 attgcggcaa acagcatccc gtgcaggcgg tccgtgatga ctagccgcgc gctggaaaat
      481 tccgcccatt tccggaacaa ctcccgttcc ctcattctgg ctgttacggt atggtcaact
      541 acagtggata tttttcgata gtcgtaatgg cactggatca gatacttttc gatgtttccg
      601 aggctttctt ccgccgtgat tttttcggtg tccccgcgca tgcaaagtaa tacgcctgcg
      661 cggcaggttg catttttcaa tgcatccgtg atgaagagaa caatatccgg aacgaaaaag
      721 gttggaattg aacggaactc cctttttaga agccgatcgg tatccgaatc tcttgcaaaa
      781 agcgtgagct ttctatgaga acaataaatc ttcgccgatt tttccagttc tgctcttcca
      841 aagatatcgt tggaataata cgcggtttgt gggaaaacaa aaatcgggtt tttcggaaat
      901 cttagaatga tatcccgcat tttgttttct tcttcgatcc acagggttcc gatatttccg
      961 ccgccgtcaa taataaggac atcatccggt gtgatcaccg ttttcagctg ctcccgacaa
     1021 aactcatatt gctgccgcgt tagctcaaat accctgtcgc caacgccgat ctgctccata
     1081 aaccggtgtt gagcatatac aattgcgtgg tccccgaggt ttccgtgcgt cggtgtggca
     1141 atgaatatta caatgcgccc atgctttctt ttaaattttt tcaagtgaat taagatggct
     1201 ttacgtcgaa taaggcgttt gattctactg caccattgaa acggtatggc tttccggatc
     1261 gaaaggcgcg tcattttccc acctgaacgg gcggcaaagt ccgccgttcc gtatccggtc
     1321 ggaacagagc catccaaatg tggcggacat aagggaataa atagaatcgc accaaaatta
     1381 gctggaaggg aaacctcttt gatagatatg ggttttttct ccaaccagac gcgagctgta
     1441 atattttttt ccgcaattca ggtactaagg accgcaggga atagaattcc ccgtactggt
     1501 aaagcagcat caacaaagct gcgctctgtg cctgatgaaa atgagacccg gtttcttttg
     1561 caaactgaag gtagctgtcg gcaacggcga tacgatcccg gatttttttc gcatcaaccg
     1621 gctgcgccat gatgctcccg tcccgctgca ggtaatggta aagaggcctg ccgatcaaag
     1681 cgactcgttc ggatttgtaa agcagccggt tcgtggtgca gttatcttca aaaaaccgac
     1741 cgaccggata gcggatgtca ttattaaaaa aaagctttct ggcgtacagt ttattccagg
     1801 caattactaa atacacaaaa ccgtttctca agaattggtc caatgcctga tctcccgtaa
     1861 gaattccgtc gggtacatga tccaccggaa atatttgaga accgtcagaa cgttccagaa
     1921 agtaatcgca caccgcaatt tccgctcccg attccgagca cgacctttca agctgttcca
     1981 gcatatccgg ctcgaccgag tcgtctccgt cgataaaacc tatgtattcg cctgacgacg
     2041 cctcaattcc cgcgtttcgt gcagaggaaa gcccgccgtt ggccttatga atcacacgga
     2101 cacgggggtc ctcctttgca tacctgtcgc agataagagg gcagtaatcc ggagatccgt
     2161 cgtccaccaa tagaatttca aggttttggt aggtctgatg aagaatgctg tccacacact
     2221 tttttaagta tttctcaacc ccgtaaacag gaacgacaac actgatcaaa ggctgcatgc
     2281 taacgcccct ttccctcaat tgccgattct tagcctcccg catcgcctga ctcattctac
     2341 aaacccgatt ttatgatcga gatcctttgc catgctttct ttcctctcat ccgtaatccg
     2401 gccgatggtc cattccctgt cgtaaacgca ggcatgattc atccgctctt tttcctgatc
     2461 cagatattcc gccg
//
LOCUS       DATALY010000212         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_650596,
            whole genome shotgun sequence.
ACCESSION   DATALY010000212 DATALY010000000
VERSION     DATALY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650596"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VHR42_06105"
     CDS             <1..624
                     /locus_tag="VHR42_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026783.1"
                     /translation="SIPRGRIVGLLGPNGSGKSTLIKLINGLLTPNSGEVLVKGAQPG
                     IESKKIVSYLPERTYLNDWMSVETMIEFFRDFYPDFNSSKAYDMLQRLHINPNDRLKT
                     MSKGTKEKVQLILVMSREAELYVLDEPIGGVDPAARDYILDTIIGNYNENATVLISTH
                     LITDVEKVLDDVIFINMGNIVLTSTVDDIREKENKSVDALFREVFKC"
     gene            618..1412
                     /locus_tag="VHR42_06110"
     CDS             618..1412
                     /locus_tag="VHR42_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026784.1"
                     /translation="MLGKLMKYELKATARIFLPLYALIIPMSLLTKIFLHTTADSFEI
                     PRMITMSVYVILMVALFVMTLIVTIQRFYRNLLGDEGYLSFTLPVKTHTHIDAKLLAT
                     LIWVMVSSLISVLSIFILAADGQTLSQLGHWWVRCVNPFLEKYGAQVWIGVIESILLV
                     LVSTLCQTAEIYAAITVGNLSSKHKLIAGIGAYIGFGVVTQTISSILVDVFKPYFGSF
                     FTRVNFDTPFPAVNYILLIILIGELVYLGIFYFLTEWLLSRKRNLE"
     gene            1560..2477
                     /locus_tag="VHR42_06115"
     CDS             1560..2477
                     /locus_tag="VHR42_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026785.1"
                     /translation="MSETAIRAEELGKTYDGVSFALRKLSLEIPQGSIFGFLGPNGAG
                     KTTTVKLLTGLLKPTEGSCELLGLSPVKKTSEVHRICGVMTETAKSYGHLTGTQNLIF
                     YGRVSGMNQQESENRAEELLKLLDLWDAREKKLREYSTGMAQRLSLARALVNRPKILF
                     LDEPTSGLDPESSQTVNALILKLARSEGTTVFLCTHQLRYAQDLCESYGILHKGQMLA
                     GGSLEALRREAGVPMRAVFRLREGQVQEGFSRQPDGWWQTEIKSDEEMPKLLKDVVLS
                     GHDVFEARMIRPTLEDVYFKLVERKETLA"
     gene            2474..3271
                     /locus_tag="VHR42_06120"
     CDS             2474..3271
                     /locus_tag="VHR42_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3026786.1"
                     /translation="MTLITDAERALAEKDFHEVWDSRMTRTTLIIVPIVMVVFLPIMF
                     LVIAYTVPTSQINGMEQMMKLLPPEAKNFTIQQDIFYLMTNLMCPMLFLMIPLMASSV
                     SAACSFVGEKERGTLESLLLTPMKIRQIFHAKVLGCIVISAITTVVSFVVFSAVTAVG
                     DLMLGLPFFLDWSWLVLVFLLAPGCTVFGVVFMVLVSGKSKNYMESVQTSGYIVLPVV
                     LLFIGQLTGLFRLGAFALLCVSLALFVLDAILWTVCSRLFTPDKLLR"
     gene            3336..4172
                     /locus_tag="VHR42_06125"
     CDS             3336..4172
                     /locus_tag="VHR42_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3026787.1"
                     /translation="MTADLHCHTKMSDGSVGIDELILLARRSGITTLAVTDHDTFAGS
                     VRAKIFGDRRGVTVIPGVEFSTLDSSTGRKAHILCYLCDNTDRLEGLCKRTHDARHHA
                     AELMLRKVMQIYPIPAEMVMKRAQGSTNLFKQHIMHALIDAGYADEFFGEVFHKLFNS
                     KDGLAYEPVAYPEVHDVISQIHEAGGVAVLAHPGEYDSYGLLEQLARDHEISGVEAWH
                     PRNRPGDEQRFRILAGEYGLVMTGGTDFHGLYTSRPVPLGTCSTPEDQLQNLIARKKK
                     QN"
     gene            4191..4451
                     /locus_tag="VHR42_06130"
     CDS             4191..4451
                     /locus_tag="VHR42_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HEX3026788.1"
                     /translation="MTYSYKTHGTCSSEIILDLEGDVVRSVRIIGGCSGNLQGISSLV
                     QGMKADDVIARLKGIRCGSKPTSCPDQIAAALEKARVLQEKE"
BASE COUNT         1069 a         1127 c         1263 g         1204 t
ORIGIN      
        1 tctatcccga ggggaaggat tgtgggactt ctggggccga acggcagcgg aaaaagcacg
       61 ctgattaagc ttatcaacgg cttacttacc ccgaacagcg gtgaagtact ggtaaagggc
      121 gctcagcccg gaattgagag caaaaagatc gtttcctacc ttccggagcg gacttacctg
      181 aacgactgga tgagcgtaga gacgatgatc gaattcttcc gggattttta cccggatttc
      241 aattcttcaa aagcttacga tatgctgcag cggcttcaca ttaaccccaa cgaccgcctg
      301 aaaaccatgt ctaagggcac caaggaaaaa gttcagctga ttcttgtcat gagcagggaa
      361 gcggagcttt atgtgcttga tgagccgatc ggcggcgttg acccggcggc tcgggattat
      421 attcttgata ccatcatcgg taattacaac gagaatgcaa ccgtactgat ctcgacccac
      481 ctgatcacgg atgtagaaaa agtgctggac gacgtcattt tcatcaacat gggaaatatc
      541 gttcttacct ccacggtgga tgatatccgc gaaaaggaga acaagagcgt ggatgcgctt
      601 ttcagggagg tattcaaatg ctaggcaagc tgatgaaata tgagctcaag gctactgcgc
      661 ggatttttct accgctgtat gccctgatta ttcccatgtc cctgctaacg aagatctttt
      721 tgcatacgac cgccgattcc tttgaaattc ccagaatgat taccatgtcc gtttatgtca
      781 ttttaatggt ggcccttttt gtcatgacgc tgattgtgac catccagcgt ttttaccgca
      841 acctgctcgg tgacgagggt tatctctcct ttactcttcc ggtcaaaacg catacgcata
      901 ttgatgcaaa gctgctcgca actctgatct gggtaatggt aagctctctg atttcggtac
      961 tgtcgatttt catcctagcg gctgacggcc agacgttatc ccaattaggc cattggtggg
     1021 tgaggtgtgt caatcctttc ctggaaaaat acggagccca agtctggatt ggcgtaattg
     1081 aaagcatact ccttgtactg gtttccaccc tgtgccagac agcggaaatc tatgcggcga
     1141 tcaccgtggg aaatctcagc agtaagcata agctgattgc cggtatcggg gcttatatcg
     1201 gattcggtgt cgtgacgcag acgatttctt ctatccttgt cgatgtgttc aaaccgtatt
     1261 tcggaagttt ctttacgaga gtcaattttg atactccgtt tccggccgtg aattatatac
     1321 tgctgatcat tctgattggc gaactggtct atctgggaat cttctatttc ctgacggaat
     1381 ggcttctcag cagaaagcgg aacctggaat aagaaaaagc acaaacgcaa atacaaatga
     1441 atcataggca agttcgggac gtacaatcgg tgcgtccctt tgccgttttt tataagaaat
     1501 cattccgcgg agtggatgat tgggcaaaaa tgtgatattc ttttttttga ggtgattgta
     1561 tgtctgaaac ggcgattcgg gcggaagaac tcggcaagac ttacgacggg gtctcctttg
     1621 ccctgcgcaa actttcgctg gaaattccgc aggggagcat tttcggcttt ttgggtccca
     1681 atggggcggg aaaaacgact acggttaagc tgctgaccgg gctgttgaaa ccgacggagg
     1741 gctcctgcga gctgctcggc ctgtctcctg tcaaaaagac cagtgaagtg catcgaatct
     1801 gcggggtcat gaccgaaacg gcgaaatcat acggccacct gaccgggacg cagaatctga
     1861 ttttttatgg ccgggtttcc ggcatgaatc agcaagagag tgaaaatcgg gcggaagagc
     1921 ttttgaagtt gctggatctg tgggacgcgc gggaaaaaaa gctcagggaa tattctaccg
     1981 gtatggcaca gcggctttcg cttgcccgtg cgctggtaaa ccgcccgaag atcctgtttc
     2041 tggacgagcc aaccagcggt cttgacccgg aatcctccca gacggtgaac gctctgattt
     2101 tgaaattagc ccgttcggaa gggactacgg tgttcctctg cacccatcag ctgcgttacg
     2161 cgcaggatct ttgtgaaagc tacggaatcc tccataaagg gcagatgctt gccggcggtt
     2221 cgctggaggc gcttcggcgt gaggccgggg tcccgatgcg cgctgtcttc cgccttcggg
     2281 agggacaggt tcaggagggc ttttccaggc agcctgacgg ctggtggcag accgaaatca
     2341 aaagtgatga ggagatgccg aagcttttga aagacgttgt tctttcgggg cacgacgttt
     2401 ttgaggctcg tatgatccga ccgacgctgg aagacgttta ctttaagctt gtggaacgaa
     2461 aggagaccct cgcatgactc tgattaccga tgcggagcgc gcgctcgcag aaaaggattt
     2521 tcacgaggtg tgggattccc gaatgacgcg caccaccctg attattgtgc cgattgtcat
     2581 ggtcgttttc ctgcccatta tgtttcttgt tatcgcttat accgtgccga caagtcagat
     2641 caacgggatg gaacagatga tgaagctgct tccgccggag gcgaagaact tcacgattca
     2701 gcaggatatc ttttatctga tgaccaatct gatgtgcccc atgctgttcc tgatgattcc
     2761 gctgatggcc tccagtgttt cggcggcctg cagctttgtg ggcgaaaagg aacgggggac
     2821 actggaatcc ctgctgctca cgccgatgaa gatccgtcag atctttcatg cgaaggtgct
     2881 gggctgtatc gttatctccg caattaccac cgttgtttcg tttgtcgttt tttccgctgt
     2941 gaccgccgtc ggggatctta tgctaggtct tccttttttt ctggattgga gctggcttgt
     3001 ccttgttttt ctgctcgcgc ccggctgcac ggttttcggt gtcgtcttca tggtgcttgt
     3061 ctccggaaaa agcaaaaatt acatggaatc cgttcagacc tccggttata ttgtattgcc
     3121 ggtcgtcctg ctgtttatcg gtcagctgac cggacttttc cggcttggcg cttttgcgct
     3181 tctgtgtgtt tcccttgcgc tttttgtgtt ggatgcgatc ctctggaccg tatgctcgcg
     3241 tttgtttaca ccggataaac tgcttcggta aaggggagcg gttgccttcc gacctgaatt
     3301 gtattataat aaagtgagaa acgcgaggtg atttcgtgac tgcagatttg cactgtcaca
     3361 caaaaatgtc cgatggttcc gtcggaattg acgagctgat attattggcc cgcagaagcg
     3421 gcataacgac ccttgccgta acggatcacg atacatttgc cggctcggtt cgggccaaaa
     3481 ttttcggaga cagacgaggg gttaccgtta tccccggcgt tgaattttcc acactggatt
     3541 cctcgacggg ccggaaggcc catattttat gctatctctg cgacaatacc gaccgactgg
     3601 agggcctttg caaaagaact cacgacgcaa ggcaccatgc ggcggaactg atgcttcgca
     3661 aggtgatgca gatttatccc atccccgccg agatggtgat gaaacgggcg cagggaagca
     3721 cgaacctttt caagcagcat attatgcacg ctctgatcga tgctggctat gcggatgagt
     3781 ttttcggcga ggtctttcat aaattgttca attcaaagga cggtctggct tatgagccgg
     3841 ttgcctaccc ggaagtgcac gacgtgatca gtcagatcca tgaggccggg ggagtcgccg
     3901 ttctggccca tcccggggag tatgacagct acggtcttct ggaacagctt gcccgcgatc
     3961 atgaaatcag cggggttgag gcatggcatc cgcgcaatcg acccggagac gaacagagat
     4021 tccggatact tgccggggaa tatgggttgg ttatgaccgg cggcacggat ttccacgggc
     4081 tttatacaag ccgtccggtt ccgcttggaa cctgttctac gccggaagat cagcttcaga
     4141 atcttatcgc gcgcaagaaa aaacagaact agagaatagg agaaaatcac atgacttatt
     4201 cctacaaaac gcacggtact tgttcttcgg agattattct ggacctggaa ggcgacgtcg
     4261 ttcggtctgt tcggattatc ggaggctgca gcgggaatct acaggggatt tcctctctgg
     4321 ttcagggaat gaaagcggat gacgttatcg ccaggctgaa gggaatccgc tgcggttcaa
     4381 agccgacctc ctgcccggac cagatcgcgg ccgctctgga aaaggcccgc gttcttcagg
     4441 aaaaggaata aggcaagggc tgcgattcat tcgcagccct ttttgaaaat cagcagccac
     4501 agccattgtt gttgcagcca tcgccgcaac cgccgcaacc gcggccggat tctttccgca
     4561 gtactggcac agcatgacag ttgcccccat aaaatagatt ttagccttat tatatcacga
     4621 tttcggtcgg atgaaaaagt cggttcaaat ttcacattcc att
//
LOCUS       DATALY010000213         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_306541,
            whole genome shotgun sequence.
ACCESSION   DATALY010000213 DATALY010000000
VERSION     DATALY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306541"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..490)
                     /locus_tag="VHR42_06135"
     CDS             complement(26..490)
                     /locus_tag="VHR42_06135"
                     /inference="COORDINATES: protein motif:HMM:NF020483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HEX3026789.1"
                     /translation="MGTATQPTAANLSDAIDIGLLDLTKAEFFVTPGGFTGLKYGDKE
                     YSRITLRRALPIGHPMEYISVADKDNKEIGMIRSVLDLSEQPRRIVVNELENRYYCPV
                     VQEVKSVKDNLGYVYFELVLGHDDETYEKNCAIKDVNKNIRVLDNDRLIILM"
     gene            complement(524..2785)
                     /locus_tag="VHR42_06140"
     CDS             complement(524..2785)
                     /locus_tag="VHR42_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026790.1"
                     /translation="MKSNIPEYIQSAFSKQGIDPASLIYAIHTDLTPNEDSADVYTAV
                     SKDSLYILYGEEKVVKVSGARRIVAVYDAHKLEQYPLDELGDLKSERLLSTGRLFSEK
                     DGEDKILMLFSVGFLSDVERLIKVTQNIKNGVLPLTDVMIDNELFCPKCGTRYPEPER
                     KLCPKCMDKVSITRRLMGFFGNYKRKVASILLTMLAGSAFSIVSPLVGSKFLFDDVLT
                     ESGKFYGMILGAVLLIFFVRAVTVALNILYSYVLARTVPWIVYNLKLNIFEAMQRLSV
                     GFYTSKRTGSLMNRVNQDANNIYWFFVDGFPYVVVNLITFTGVVTMMCLLNIKLALIC
                     VFILPVTIGLFKILWSVFRRYHHKNWVYSSQLNSMVSDSVNGQRVIKAFAKEDDEAKR
                     FSEVGNKLTAVDVKSMNTGYTAFPLIYLFMFLGQVIVTAVGSMMVINREMTLGTLLTF
                     ISYLSMLYGPLEFMSWVSNWWARCVDSAQRVFEIIDARPDIADPENPVVLDTVQGNIE
                     IHNVKFEYDPATPVLKGLNLSVKAGEMLGIVGKTGAGKSTLANLIARLYDVTEGSITI
                     DGVNVKQLSLKQLRQSIGIVSQDIYLFIGTIADNIRYSKPDASMEDVIRAAKAASAHD
                     FIMKLPDAYETKVGAGGQDLSGGEKQRLSIARTILQNPSILILDEATAAMDTETEANI
                     QNSLSKLQKNRTTIAIAHRLSTLRDADSLAVISEGRVIESGTHKELMLKKGEYHKLYT
                     IQLEGLKVINMDA"
BASE COUNT          748 a          668 c          615 g          776 t
ORIGIN      
        1 gaattgatca tataccggtt tccgtctaca tcaaaatgat gagcctgtca ttatcaagaa
       61 cgcgaatatt tttatttacg tcttttatag cgcagttctt ttcataggtt tcatcgtcgt
      121 ggccaagaac tagttcaaaa tagacatatc ccaagttatc tttgaccgat tttacttcct
      181 gtacaacggg gcagtaataa cgattttcta gttcgttcac gactatacgg cgcggctgtt
      241 cactcaggtc aagaaccgaa cggatcatcc cgatttcttt gttgtctttg tccgctaccg
      301 agatatattc catcggatgg ccgatgggca gcgcccgtct taatgtaatg cggctgtact
      361 ctttgtctcc gtattttaac cctgtaaatc ctcccggtgt aacaaagaat tcagctttag
      421 tcaggtccaa taagccgatg tcaatggcat cggataaatt tgctgcagtt ggttgtgtcg
      481 ctgttcccat ataattccct cctaaatagg tgatatgatg ttgttatgcg tccatattaa
      541 tgactttcag accctccagt tgaatggtat aaagtttatg gtattcgcct tttttcagca
      601 tcagttcttt atgcgttccg ctttctataa ccctgccttc actgataacg gcaaggctat
      661 cggcgtcccg caaggtggaa agccgatggg ctatggcaat cgtcgtgcgg tttttttgca
      721 gtttggataa ggaattctgt atattggcct cggtttctgt atccattgcc gcggtcgctt
      781 cgtctagaat caatatggag ggattttgca atattgtgcg tgcgatagac agacgctgct
      841 tttcgcctcc agagagatct tggcctcccg cgcccacctt tgtttcataa gcgtccggaa
      901 gcttcatgat aaaatcgtga gccgacgccg ctttggcagc acggatgaca tcctccatgc
      961 tggcgtctgg ttttgaatac cggatgttgt cggcaatggt tccgataaag aggtagatat
     1021 cctgcgatac gatgccgatg ctctgcctta attgtttcag agaaagctgt ttgacattga
     1081 caccgtcaat tgtaatagag ccttccgtta cgtcataaag ccgggcgata agattcgcca
     1141 gagtggactt gcccgcaccg gttttgccga cgatgcccag catttcccca gctttcaccg
     1201 acaaattcaa tccttttaaa accggtgtgg cggggtcgta ttcaaacttt acgttgtgta
     1261 tttcaatatt gccttgaaca gtatccaaca caaccggatt ttcaggatcg gcaatatccg
     1321 gcctggcgtc gatgatctca aaaacacgct gagccgaatc tacacagcga gcccaccagt
     1381 tagataccca ggacatgaat tccagaggtc cgtacagcat ggacaggtaa gatataaatg
     1441 tcaatagagt gcctaaagtc atctctctgt taattaccat catgctgccg acagccgtta
     1501 ctatcacctg ccccaaaaac ataaacagat aaatcagcgg gaaggctgta tagcctgtat
     1561 tcattgattt gacatcgaca gcggtaagct tatttcccac ttctgaaaaa cgtttggctt
     1621 catcatcctc ttttgcaaac gctttaataa cgcgctgacc gtttacagag tcgctgacca
     1681 tcgaattcag ctgtgagctg tatacccagt ttttatgatg ataacggcga aaaacgctcc
     1741 acagaatttt aaacagaccg atcgtgaccg gcaaaatgaa tacacagata agcgccagtt
     1801 tgatattcag caggcacatc attgtgacaa cgccggtaaa ggtaattaaa ttaacgacga
     1861 catagggaaa accatccacg aaaaaccaat agatattatt ggcgtcctgg ttgacgcggt
     1921 tcatcagcga accggttcgc ttggatgtat aaaacccgac tgacagccgc tgcatggcct
     1981 caaaaatatt tagtttcagg ttgtatacga tccagggaac ggtccttgcc aaaacataag
     2041 aatacaggat attcaaagcc acggtgactg cccggacaaa gaaaattaaa aggacggcgc
     2101 ccaatatcat accgtaaaat ttgccgcttt cggtaagtac atcgtcaaaa agaaattttg
     2161 agccgacaag gggggagacg atggaaaacg cggaacccgc cagcatagtc agcagaatgg
     2221 acgccacttt acgcttgtaa tttccaaaga accccattag ccggcgcgtt atcgaaactt
     2281 tatccataca tttcggacac aattttctct ccggctccgg ataacgcgta ccgcatttcg
     2341 ggcagaacag ttcattgtca atcatgacgt cggtaagcgg caggacgcca tttttaatat
     2401 tttgtgtaac ttttatcaat cgttcgacat ccgataaaaa accgaccgag aagagcatca
     2461 gtatcttatc ttcaccgtct ttttcactga acagccgtcc cgtggaaagc agccgttcac
     2521 ttttcagatc gcctaactca tcgagaggat attgttccag cttatgcgca tcgtaaacgg
     2581 cgacaatccg gcgggcaccg ctgactttga ctactttttc ttcgccatat agaatataca
     2641 ggctgtcctt gctgacagct gtatagacat cggcactgtc ttcgttcggg gtcaagtcgg
     2701 tgtgaatcgc gtagatgaga gatgcagggt cgataccctg tttggaaaaa gccgactgga
     2761 tatattccgg tatgtttgac ttcatcaaaa tcccctccta aaaagaa
//
LOCUS       DATALY010000214         4846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_478412,
            whole genome shotgun sequence.
ACCESSION   DATALY010000214 DATALY010000000
VERSION     DATALY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478412"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /gene="pgk"
                     /locus_tag="VHR42_06145"
     CDS             <1..285
                     /gene="pgk"
                     /locus_tag="VHR42_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX3026791.1"
                     /translation="AALFADAIKGTGTVVWNGPMGVSEWDNFAAGTIAVAKAVADSGA
                     ISIIGGGDSAAAVEKLGFAEKMTHISTGGGASLEFLEGLELPGIACLNDK"
     gene            357..1124
                     /gene="tpiA"
                     /locus_tag="VHR42_06150"
     CDS             357..1124
                     /gene="tpiA"
                     /locus_tag="VHR42_06150"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX3026792.1"
                     /translation="MNKKLRKAVIAGNWKMNKTPAEAKELIEGIKPLVKDADCDVVVC
                     VPYVDLSTALEATKGTNIGVGAENCHWEKSGAFTGEISADMLTSMGVQYVIIGHSERR
                     TYFGETDVTVNKRVRAALDSGLTVILCVGEYLEQREQGITDELVAMQTKIALGGVSKD
                     ELKKIIIAYEPVWAIGTGKTATSEQANEVCAVIRATVKKLYDAAAADAMTVQYGGSMN
                     AKNANELLDQPDVDGGLIGGASLKPADFAEIVKAASR"
     gene            1212..2723
                     /gene="gpmI"
                     /locus_tag="VHR42_06155"
     CDS             1212..2723
                     /gene="gpmI"
                     /locus_tag="VHR42_06155"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX3026793.1"
                     /translation="MKKPLLLMILDGFGIAPENGNAIKAAHKPNLDRLFTNNPITQIG
                     ASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQELTRITKSVSDGDFFTNPALLAAADNA
                     AANNKALHLIGLLSDGGVHSHNSHLYALVELAKKRGCKKVYVHALLDGRDVPPSSGKD
                     FVAECAQKLREIGVGEIATVMGRYYAMDRDNRWERVEKAYAAMVYGEGVPCADPVKAV
                     ENSYRDEVTDEFVVPTVCAKGAGIQANDSVVFFNFRPDRAREITRTFVDPEFKGFERK
                     KGFFPLTYVCMTQYDATMPNVQVAFKPQSLENTFGEYISKNGLTQLRIAETEKYAHVT
                     FFFNGGVEKQYSGEDRVLVNSPKVATYDMQPEMSAYEVTDKLVDCIHSGKYDVIILNF
                     ANCDMVGHTGVFEAAKAAVEAVDKCVGRVTDAISEMGGVALITADHGNADKMFEDDGS
                     PFTAHTTNPVPFCVVGYPCTLRSGGRLADIAPTMLKILGLPQPAEMDGKSIVE"
     gene            3087..3992
                     /locus_tag="VHR42_06160"
     CDS             3087..3992
                     /locus_tag="VHR42_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026794.1"
                     /translation="MKKTILFLLKILLLTIILFVCMAASSILMGMGQTFSSSSSDVMP
                     VLMYSLLVYSLLNVLILTVWIENSRLRGIRLAVVCFTVFWGVQYFMTQIETLFFNSAV
                     KMPVSEVFRVSSSGALYTAVFSMLAVWILGKFKKENARNEITGEKAARFALLPNLAVL
                     TLTYVVIYFLFGYFVAWQFPVIRQFYTGSTQIVNFFQHMAGQSPFLILFQLFRGILWA
                     LLALLILNSLKVKNRASYLIPGLLFSILISAPLLLPNAYMPDAVRFGHTIELFSSMLT
                     YGILSVPILKKRSSGSSKINALSQG"
     gene            complement(4073..4459)
                     /locus_tag="VHR42_06165"
     CDS             complement(4073..4459)
                     /locus_tag="VHR42_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HEX3026795.1"
                     /translation="MANKNAKFFICKHCGNFVGMIYNSGASMICCGEPMTELIPNTAE
                     AAHEKHLPVVTVSGNQVTVKIGSVEHPMTEAHYIQWIYLETKHGGQRKALNPGDKPEA
                     VFVLEDDEAVAAYAYCNLHGLWETKI"
     gene            complement(4603..4815)
                     /locus_tag="VHR42_06170"
     CDS             complement(4603..4815)
                     /locus_tag="VHR42_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026796.1"
                     /translation="MNEELLKKAIRYKLELADSMLDCLPPKMSSEIKNLRRAAAECLN
                     DYEKEAHSPASPRENPDGGLKHVPIE"
BASE COUNT         1184 a         1221 c         1199 g         1242 t
ORIGIN      
        1 gccgcactgt tcgcggatgc cataaaaggc accggaacgg tcgtatggaa cggacctatg
       61 ggcgtttccg aatgggataa ctttgccgca ggcaccatcg ccgtggcaaa agctgttgcg
      121 gacagcggcg caatctccat catcggcggc ggcgattccg cggctgcagt tgaaaagctc
      181 ggttttgcgg agaaaatgac ccatatttcc acgggcggcg gcgcttccct tgaattcctt
      241 gagggccttg agctcccggg tattgcctgc ctgaacgata aataataatc gatagcctga
      301 ggggcggagg tgttccgccc ctttaattct atatattaag atatggagga cttaaaatga
      361 ataagaaatt aagaaaagca gttatcgcgg gtaactggaa aatgaacaag acccctgccg
      421 aggcgaagga actgattgag ggcattaagc cgctcgttaa ggacgcggac tgcgacgttg
      481 tcgtatgcgt accttatgtt gacctctcca ctgcactgga agcaaccaag ggcaccaaca
      541 tcggcgtggg cgccgagaac tgccactggg aaaagagcgg cgcgttcacc ggtgaaattt
      601 ccgcggatat gctgacttcc atgggcgtgc agtacgtaat catcggccac agcgaacgca
      661 gaacctactt cggcgagacc gacgtgaccg taaacaagcg cgtacgcgca gctctggaca
      721 gcggcctgac cgtaatcctc tgcgtaggcg aataccttga gcagcgcgag cagggcatta
      781 ccgacgagtt ggttgccatg cagacaaaga tcgcgctcgg cggcgtttcc aaggatgagc
      841 tcaagaagat catcatcgcc tacgagcctg tatgggcaat cggcacgggc aaaaccgcta
      901 cctccgaaca ggcaaacgaa gtctgcgccg tcattcgcgc aaccgtgaag aagctgtatg
      961 acgctgccgc ggcagacgcc atgactgtcc agtacggcgg ttccatgaac gcaaagaacg
     1021 caaacgagct gctcgaccag cccgacgttg acggcggcct catcggcggc gcttccttaa
     1081 agccggccga ctttgctgag atcgtcaaag cggcaagccg ataaataaat attaatgata
     1141 cggaatcctg tttggtgccg cacagcatcc aaataggatt cttatttccg aaaggcagga
     1201 gctcattcac tatgaaaaaa ccactgttat taatgatact ggacggcttc ggcattgcgc
     1261 cggaaaacgg caacgctata aaggccgcgc acaagccgaa ccttgaccgt cttttcacga
     1321 acaacccgat cacgcagatt ggggcttccg gtctggacgt aggtctgccg gacgggcaga
     1381 tgggcaactc cgaggtcggc cacaccaaca tcggcgcggg ccgcatcgtt tatcaggagc
     1441 ttacccgcat tacaaaatca gtcagcgacg gcgacttctt tacgaacccc gcgcttcttg
     1501 ccgcggcaga caatgccgca gcgaacaata aagcgctgca cctcatcggc ctgctctcgg
     1561 acggcggcgt tcacagccac aattcacatc tgtacgccct ggttgaactt gcgaaaaaac
     1621 gcggctgcaa aaaggtctat gtccacgcgc tgcttgacgg ccgtgatgtt cccccttcct
     1681 ccggcaagga ctttgtggca gaatgcgcgc agaagctccg ggaaatcggc gtgggtgaaa
     1741 ttgcgaccgt gatgggccgc tactacgcga tggaccgcga caaccgctgg gagcgtgtgg
     1801 aaaaagcata cgccgccatg gtttacggcg agggtgttcc gtgtgccgac ccggtaaaag
     1861 ccgtggagaa ttcctatcgg gacgaggtta ccgacgaatt cgtggttccg accgtatgcg
     1921 caaagggcgc cggaattcag gcgaacgatt ccgttgtgtt cttcaacttc cgcccggacc
     1981 gtgcgcgtga gatcacccgc acctttgtgg acccggaatt caagggtttt gaacgcaaaa
     2041 aaggattttt cccgcttact tacgtctgca tgacgcagta cgatgccacc atgccgaatg
     2101 ttcaggttgc gtttaaaccg cagtcgctgg aaaacacctt cggcgaatat atttcaaaga
     2161 acggcctcac ccagctccgc atcgcggaaa ccgaaaaata tgcccatgtg accttcttct
     2221 tcaacggtgg cgtggaaaag cagtattccg gtgaagaccg cgtgctggtc aactcaccga
     2281 aggttgcaac ctatgatatg cagccggaaa tgagcgcgta cgaggttacg gacaaactgg
     2341 tcgactgcat ccacagcgga aagtatgacg taatcatatt gaactttgcc aactgcgaca
     2401 tggtcggcca taccggcgta ttcgaagccg cgaaagccgc ggtggaagcg gttgacaaat
     2461 gcgtgggcag agtgaccgac gccatttccg aaatgggcgg cgtggccctg atcaccgcgg
     2521 accacggcaa cgccgacaag atgtttgagg acgacggttc cccgtttacg gcacatacca
     2581 caaacccggt tccgttctgc gttgtgggct atccgtgcac cctccgctcc ggcggcaggc
     2641 ttgccgatat cgccccgacc atgctcaaaa ttctcggcct gccccagccg gctgaaatgg
     2701 acggcaagag cattgttgaa taatttaaca gaaattttaa aggctctttc tcaaatgaga
     2761 aagagccttt tgtgtgaatg tatggagatt tatactactg agtgccgtga gaatcaatga
     2821 atttgcaatc taaccgttat agaacaggtc aattttttat taaagcaatg gctcttagtg
     2881 gaagggaaag aaattcgatt tggtctgcgg atttggacgg catagtaatt cttaattcta
     2941 ttttaatttc atataaattt ttcttaaagt cttatttatt agaatgttat catacgataa
     3001 tgtggaagga actgtacttt aaactgtgca caccggacgt ctgaatcgta attaacttaa
     3061 taaaaagatt taaggggtta atatttatga aaaaaacaat ccttttttta ctaaaaattc
     3121 tcctgctcac cataattcta tttgtatgca tggcggcttc cagtatacta atgggtatgg
     3181 gacagacttt ctcgtcttcc tcaagcgacg ttatgcctgt tttgatgtat tctcttttgg
     3241 tgtattctct tttgaatgtt ctgattctta cagtatggat tgaaaattca agactgcgtg
     3301 ggatacgact tgcggttgtg tgttttaccg tattttgggg cgtccagtat ttcatgacac
     3361 agattgaaac cctgtttttt aacagtgcgg taaaaatgcc tgtatcagaa gtgtttcgcg
     3421 tatcgtcatc gggcgccctg tatacggcgg tattttctat gctggcagtc tggattcttg
     3481 gaaaatttaa aaaagaaaac gctcggaacg agataactgg agagaaagca gcaagatttg
     3541 cattacttcc caatcttgcc gttctaactc tcacctatgt cgtaatctat tttctgtttg
     3601 gatattttgt agcttggcaa tttcctgtaa ttcgtcaatt ctatactgga agcacacaga
     3661 ttgtgaattt ctttcagcac atggccggtc aaagcccgtt tctgattttg ttccagcttt
     3721 tccgggggat tttgtgggct ctgctggcac ttctaatctt gaattctctg aaagtgaaaa
     3781 accgtgcatc ctacttgatt ccgggattgc ttttcagcat tttgatttca gccccgctgc
     3841 tactccccaa cgcatacatg cccgatgccg ttcgtttcgg acataccata gagcttttta
     3901 gttccatgct gacttatggg attctttccg taccaatact gaagaaaaga agcagcggga
     3961 gttcaaaaat caatgcactc tctcagggtt gaccgttcat gacagtatct acatttaaaa
     4021 ccacagcggt tctctccaat tggaaagaac cgctgttttt gggttattaa aatcagattt
     4081 ttgtttccca caggccgtga agattgcaat aggcataggc cgcaacggct tcatcgtctt
     4141 caagtacaaa gaccgcctcc ggcttatccc ccggattgag cgccttgcgc tggccgccat
     4201 gcttggtttc aagataaatc cactgaatgt agtgcgcttc cgtcatggga tgttccacgc
     4261 tgccgatttt tacggttacc tgattgcccg aaacagtgac caccggcaga tgcttttcat
     4321 gtgccgcctc cgccgtgttc ggaatcagct ccgtcatcgg ttcgccgcag cagatcattg
     4381 atgctccgct gttgtaaatc attcctacaa aatttccaca atgcttacag ataaaaaact
     4441 tcgcattttt gtttgccata ctgctttcct ccattcctta ttttggtatt cctattatac
     4501 cttaatttat attaatatta aatactattt ttgaattgtt ttgccgcgca gtaattcccc
     4561 ttgagccggc tgcttctgcc gcccaagggg gatttacttg ttttattcaa ttgggacatg
     4621 cttcaggccg ccgtccggat tttcccttgg ggaagccggg ctgtgcgctt ctttttcata
     4681 atcgttcaga cattcggccg ccgccctgcg cagatttttt atctccgacg acattttcgg
     4741 cggcaggcag tccagcatgg aatccgccag ctccagtttg taccggatcg cctttttcaa
     4801 aagctcttca ttcatggctt tcccctctaa aacgtatttt cagaac
//
LOCUS       DATALY010000215         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_133056,
            whole genome shotgun sequence.
ACCESSION   DATALY010000215 DATALY010000000
VERSION     DATALY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133056"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..601
                     /locus_tag="VHR42_06175"
     CDS             200..601
                     /locus_tag="VHR42_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3026797.1"
                     /translation="MSNSYVGSGGGHFEKEELNDARACVLRELNEETGLVEEDITDLT
                     LRYVTLRLKNGEIRQNYYFFANLINQDRKLSSNEGRLKWADRNDISSLDMPFTAKQVI
                     DHYISIGKNTTFLYAGIATSEGINFVKMQEF"
     gene            660..1538
                     /locus_tag="VHR42_06180"
     CDS             660..1538
                     /locus_tag="VHR42_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX3026798.1"
                     /translation="MNYLKVALLQLLPEQTLEGNLQKGLDYCKKAKKMGADLALFPEM
                     WSVGYHIPENIDELKTSAVPADSDFVTSFGKLAKALDMAIGITFLEKYEPLPRNTLRL
                     FNRFGESVLTYAKVHTCDFGDECRLTAGDDFYAANLDTAHGNVKIGAMICYDREFPES
                     ARILMLKGAEIILVPNACPMEINRISQLRARAYENMVGIATVNYPFGQPDCNGHSTAF
                     DGIAYRPSDSCSRDTLIVEAGEREGIYMADFPLDEIRKYRQHEVQGNAYRHPQKYKLL
                     VSENIDDPFIRKDYRK"
     gene            1705..2976
                     /locus_tag="VHR42_06185"
     CDS             1705..2976
                     /locus_tag="VHR42_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3026799.1"
                     /translation="MKKELKEKLWTPSFLILWQGQFVSTIGDAIYSIALGFWVLSATG
                     STALMGLLMAASTLPGVLISPFAGVLIDRSKKKRLFILMDLLRGVCIVLLSTAAYKGL
                     LAVWMVFAAGIILSLCGAVFSPGIQSAVPDLVPKSKIANANSAFATVSVGSNMIGSVA
                     GGFLYQLLGAPLLFLIDGLSFLFSGLTLPFVKIPDNKSEEKKHFFEDMADGFRFMWNQ
                     KGLRIILIIAAVINFFANIGIVLFMPLFQSTPSLGSGKYGIAMACFMAGELAGFMLFS
                     IISVKPANKLLLFVSANIIFDATMLIAVNQTSFLIMTVFLVITGFFNSIVNVLLMSTV
                     QGSTPQEVRGKVMSFWGMMTQGLCPFAMALGGVLGGIFPIRFVVSAAFAAVFLITVPA
                     YFSKPFQKYITTDFTADTKSKEMEKAEPVLE"
     gene            3144..>4639
                     /locus_tag="VHR42_06190"
     CDS             3144..>4639
                     /locus_tag="VHR42_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3825 domain-containing protein"
                     /protein_id="HEX3026800.1"
                     /translation="MDLFTCIGSCEPEYPDIPENKLKEIYHALISRFSDNQEIAMARF
                     SLAMNEAGFSSKLLGYPKMKFMLPKMQQFISYKTDPNSRVSDLLVTLHRVPEWDAQEP
                     LEQNSGSSLEINSAAEEPNEWDILPASKFGPLSPEQLAKLPDSFSSIRLTVVVQNSLL
                     GFVSGTRIQGMGALMNANQLEQLSQDYSEAKKANLFRSNEHINGAYDFPTHFRALQGD
                     LLFLTLAPTQNENDRFSWYSKFCGKVFNTSVQPGEHGTPAALELSDEFSEKAVNFNAF
                     ALSSMHCYTEGLLIDSKRILKPTPKEMQALEASYQTARQEQRFFSSNNGYVFLTTLRT
                     PNHQQLQVEIVPEYQSGKWVLTQIKPAVPKLSLERFAYMGSWDWVLKQLEGKALAEHW
                     SFGHLEDRRILQGYLRYTFYRLECEGKVLKSKDGQYAAFNTGLVDESYDDIYAVFVPQ
                     DPFNGVLWKFCAFCVQSESPWGKELVKTFNPLPKRAEYFRLSNASDLL"
BASE COUNT         1218 a         1113 c         1120 g         1188 t
ORIGIN      
        1 ggaaaaagaa gatttgtcaa aatggaactt gcgactagac accttggaga cgatgacgtt
       61 attgcagtag gtagaataac gtaatcattg aatcggaaat ggaagcggta ttttggacgg
      121 aaaactcagg aacatgtcat ccgtatattt attacacggc gacaaaatac tttttcttta
      181 tcggattggc tccagagtcg tgagcaactc ttatgtcggt tcaggcggcg gccattttga
      241 gaaggaagag ctaaacgacg cacgggcatg cgtccttcgt gaattaaacg aggaaaccgg
      301 actggtcgaa gaggatatca cggatttgac tctgcggtat gttaccttac gattgaaaaa
      361 tggcgaaatc aggcagaatt attacttttt tgccaatctt atcaatcaag accgaaaact
      421 ttccagtaat gaaggccgtt taaaatgggc agacaggaat gatatttctt ctcttgacat
      481 gccatttacc gcgaaacagg tgattgacca ctatatcagc attgggaaga acaccacttt
      541 tttatacgcc ggcattgcga cttctgaagg cataaatttt gtaaaaatgc aggaatttta
      601 gattctgaaa tacataatta aggacataac attcgaattt tgaaagagga cttaatttca
      661 tgaactatct gaaagtcgcc ctgctgcagc ttttaccgga acagactttg gaaggtaatc
      721 tgcaaaaagg gttggattat tgcaaaaaag caaaaaaaat gggagccgat cttgcgctgt
      781 tccccgaaat gtggagcgtt ggctaccaca tcccggaaaa tatcgatgaa ttaaaaacga
      841 gcgcagttcc agcagatagc gattttgtca cttcgttcgg caaacttgca aaagcccttg
      901 acatggctat cggaattact ttccttgaaa agtatgaacc gctgcctaga aacacacttc
      961 gcctgtttaa tcgtttcggc gaaagcgtgc ttacctacgc gaaggtgcac acctgcgatt
     1021 tcggcgacga atgtcggctg acggcgggtg acgacttcta tgctgccaac ctggacaccg
     1081 cacatgggaa tgtaaaaatc ggcgcgatga tttgctatga ccgtgaattc cccgaaagcg
     1141 cccggatttt gatgctgaaa ggcgccgaga tcatccttgt tccgaacgca tgcccgatgg
     1201 aaattaaccg gatttctcag cttcgggcgc gggcttacga aaacatggtg ggtatcgcga
     1261 cggtgaatta tccttttgga cagccggatt gcaacggcca ttccactgca ttcgacggga
     1321 ttgcgtacag accatccgat tcctgttcca gagacacgct gattgtcgaa gcaggggaac
     1381 gggaagggat ttatatggcg gattttcctc ttgatgagat caggaagtat aggcagcatg
     1441 aggtgcaggg caacgcctat cgccatccgc aaaagtacaa actccttgtc tcggaaaaca
     1501 ttgacgaccc attcatcaga aaagactaca gaaaataaaa aaaattcccg cctcttaccc
     1561 tctctttttg acctgtcacc gagtgacacg aaccggttag acaaaagcca tttcaatatg
     1621 gattcagtct gctacaatac attgcagagc gtaatcatcc ctgcagggtg agtaaactga
     1681 attccatatg aggtggtaaa tctgttgaaa aaagagttaa aagaaaaatt atggaccccc
     1741 agctttctga ttttatggca ggggcagttc gtttcgacaa ttggcgacgc gatttacagc
     1801 atcgcattgg gcttttgggt actctccgct acaggatcga ccgcgctgat ggggctgcta
     1861 atggccgctt ccacgcttcc aggagtgctg atctctcctt tcgccggagt actgattgac
     1921 cgcagcaaga agaagcgcct gttcatcctg atggatcttc tccgtggtgt ctgcattgtt
     1981 cttctctcca ccgcagccta caaaggatta ctcgccgtct ggatggtctt cgcggcaggc
     2041 atcatcctca gcctctgcgg cgcagtattc agccccggca tccagtcggc tgtgccggac
     2101 ctggtaccaa aatcgaaaat tgcaaatgca aactccgctt ttgcaaccgt ctcagtcggt
     2161 tctaacatga tcggcagcgt agccgggggc tttctttacc agcttctcgg tgcacctttg
     2221 ctgtttctga ttgacggatt atcgtttctg ttttcagggc tgacactgcc tttcgtcaaa
     2281 attccggaca acaagtcgga agagaagaaa cacttttttg aggacatggc ggacggtttt
     2341 cggtttatgt ggaaccagaa gggtctgcga attattctga ttattgcggc agtcatcaac
     2401 tttttcgcca acatcggcat cgtcctgttt atgccgctct ttcagtcaac cccgtccctc
     2461 ggttcaggga aatacggaat cgccatggcc tgcttcatgg caggcgaact tgcgggattc
     2521 atgctctttt ccatcatttc cgtaaagccc gccaacaagc tgcttctgtt tgtttccgcg
     2581 aatattatct ttgacgctac catgctgatt gcagtcaatc agacctcttt cctgattatg
     2641 acggtgtttt tggtcattac cggtttcttt aactccatcg tgaacgtgct cctcatgtcg
     2701 accgtgcagg gctccacgcc tcaggaagtc aggggaaagg taatgtcgtt ttggggcatg
     2761 atgacgcagg gtctttgccc gtttgccatg gcgctcggcg gagtcctcgg cggaattttc
     2821 ccgatccgat ttgtcgtttc ggccgcgttc gccgcagttt tcctgatcac tgtccccgct
     2881 tacttctcca aaccgtttca gaagtatatt acaactgact ttaccgctga tacgaaatcg
     2941 aaagaaatgg aaaaagcaga accggttctg gaatgaaaag cagtcctctc aatccgctgt
     3001 atgaatgaaa cggctcatgc ggcggcggat ttcggatttg ccttcactcg cacgggtaaa
     3061 atgggaattc agtataaacg ttgtctaaac catagtataa tgaaggaagg ttttagcggc
     3121 agaacagaag agagaggatg tatgtggatt tgttcacatg tattggatcc tgcgaaccgg
     3181 aatatcccga tattcccgaa aacaagctga aagaaatcta ccacgctttg atttcgcgtt
     3241 tttcagataa tcaggaaatt gcaatggctc ggttcagtct tgcaatgaac gaagcgggtt
     3301 ttagcagtaa attgctggga tacccaaaga tgaagttcat gctgccaaaa atgcagcagt
     3361 ttatcagcta caaaactgat ccgaacagtc gcgtcagcga tttgcttgtc accttgcacc
     3421 gagtcccgga atgggacgca caggaaccgc tggagcaaaa ctcaggcagc tctctggaaa
     3481 taaattccgc ggcagaagag ccgaatgaat gggacatcct ccccgcaagt aaatttggcc
     3541 cactctcccc agaacagttg gcgaaactgc ccgacagctt ttcgagcata cgactgacgg
     3601 tggtagtcca gaattcgctg ctcgggttcg tttcagggac acgcattcag ggaatgggcg
     3661 cgctgatgaa cgcaaatcag ttggaacagc tctctcagga ttacagtgaa gcaaaaaagg
     3721 ccaatctgtt ccgctccaac gaacatatta acggcgccta tgattttccg acgcactttc
     3781 gggcattgca gggagacctt ctttttctga cgctcgcccc aacacaaaat gaaaatgaca
     3841 gattctcgtg gtattccaaa ttctgcggca aagtgttcaa cacctccgtt cagcctggcg
     3901 aacatggtac ccccgccgct ttggaactgt ccgacgaatt ttctgaaaaa gcggttaatt
     3961 ttaacgcctt cgcgctttcc tccatgcatt gttatacgga agggctgttg attgattcaa
     4021 aacgaattct gaaaccaaca ccaaaggaaa tgcaggcttt ggaagcctcc taccaaactg
     4081 cccggcagga acagcgtttt ttctccagca acaacggcta tgtgtttctt acgacgctgc
     4141 gcacccccaa ccaccaacag ctgcaggtag aaatcgtacc cgaataccaa tcgggcaaat
     4201 gggtactgac ccagattaaa cccgctgttc ccaaactttc actcgagcgg ttcgcctata
     4261 tgggaagttg ggattgggta ttaaagcagc tggaaggaaa agcgcttgcg gagcactggt
     4321 ccttcgggca tctggaagat cggcgtatcc tgcagggata cctgcgctac acgttttatc
     4381 gtctggaatg cgagggcaag gtcctcaaat ccaaggacgg gcaatacgca gcctttaaca
     4441 ccggtttggt cgacgaatcc tatgacgaca tctacgcagt ctttgtacct caggacccat
     4501 ttaacggtgt cttatggaaa ttctgtgcct tctgtgtgca aagcgaatcc ccatggggca
     4561 aagagcttgt aaagacgttc aatcctctgc cgaaacgtgc ggaatatttc aggctttcca
     4621 atgcttccga cttgctgta
//
LOCUS       DATALY010000216         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_479259,
            whole genome shotgun sequence.
ACCESSION   DATALY010000216 DATALY010000000
VERSION     DATALY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479259"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VHR42_06195"
     CDS             <1..360
                     /locus_tag="VHR42_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026801.1"
                     /translation="VYSGLLYLMTCQHFLFQRFGMMFFTKMILTVPELFASIGVQCVQ
                     TEPLETTRNLKSYRNKAERKKAFQEKRKIKTSLNMHKYIYYYALVALVFVGFLYNVWI
                     LLANRINLVPYLTFLNK"
     gene            509..1294
                     /locus_tag="VHR42_06200"
     CDS             509..1294
                     /locus_tag="VHR42_06200"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX3026802.1"
                     /translation="MIRNRIAVSVVIAAIVLNIAGCTAKTDVSVSSLPTSSTSVQAKA
                     KSTLPHSLGLSGVENARDLGGYRTADGKTVKMGVLLRTAALEHATANDIRILTEQYHL
                     AQIIDFRTVKGARENPDPVINGVTETNLPIHPSTKQLRPGENSSYVDMVTNPKSISAF
                     RQFFTILLSHKNRAVLFHCAAGKDRTGIASILLLSALNVDENTIIQDYLLSNEYHASV
                     EKDWAMTALHEINSRYGSMDAFLNRELGLTPQDRIKLQAMYLQ"
     gene            1507..2001
                     /locus_tag="VHR42_06205"
                     /pseudo
     CDS             1507..2001
                     /locus_tag="VHR42_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          562 a          462 c          524 g          508 t
ORIGIN      
        1 gtgtattcgg gccttcttta cctcatgacc tgccagcatt ttctgtttca gcgtttcggc
       61 atgatgtttt tcacaaagat gattctgacg gtcccggaac tgtttgcgag catcggcgta
      121 cagtgcgttc agacggaacc attggaaacg acccggaacc taaaatctta ccgaaacaaa
      181 gcggaacgga aaaaagcctt tcaggagaag cgaaaaataa agaccagcct gaatatgcac
      241 aagtatattt attattatgc tctagtggcg ctggtgtttg tcggttttct ttacaacgta
      301 tggattcttc ttgcaaacag gattaacctg gttccttatc tgacctttct taacaagtaa
      361 tcgcagaacg tttttgttag caggctccgg tgggaactgg cttgctgtgg agagcggtca
      421 agtgctgtat gaacaccctg cgataggggg acactaacta agttaattca gtcaattcaa
      481 gagttgttct gaagaaaggt gggcggaaat gatacgaaat agaatcgctg tttctgtggt
      541 gattgcggct attgttttga atattgccgg atgcacggca aaaacagacg tttcggtctc
      601 ctctttgccg acgagcagca cttccgtgca ggcgaaggcc aaatccactt tgccgcattc
      661 tctggggtta tcgggagtcg aaaatgcgag agacctcggc ggctatcgca cagcggatgg
      721 aaaaacggtg aaaatgggcg tgctgctgcg gacggcggcg cttgaacatg caacggcgaa
      781 tgacattaga atactcaccg aacagtatca tctggctcag ataatcgatt tcagaacagt
      841 caaaggagcc cgtgaaaatc cagacccggt aataaacgga gtgacggaaa caaatcttcc
      901 gattcatcca agcaccaaac agttaagacc gggcgaaaat tcatcatatg tggatatggt
      961 gacaaaccca aaatctattt cagcattccg gcagttcttt acgattttac tttcccataa
     1021 aaacagagcg gttttgttcc actgcgcggc ggggaaggac agaacgggga ttgcctcaat
     1081 tctgctcctg agtgccttga atgtagatga gaacacgatt attcaggatt acctgttaag
     1141 caatgaatat catgcttccg tcgaaaaaga ttgggccatg acggcgttac atgaaatcaa
     1201 cagcaggtat ggctccatgg acgcctttct gaatcgggag cttggcctga caccgcagga
     1261 caggatcaag ctgcaagcaa tgtatttaca atgacagaat aatcattcag ccagtatgaa
     1321 cttttgatgc acggctggaa tcagtgagcc ccaattgcta tttggcaaat cgccagtttt
     1381 agcaattggg gcgaagttca ttcaggcaaa atgaaaaaac acctccgtct gaaaataccc
     1441 gcggaaacga atcggtcctc ttgagttcgc gggtgaccaa aggtataata gcagcggagg
     1501 agtaatatgg acacgaacta tatcagcagg gtggaactga cgaagccatt gcccaaagat
     1561 tcgtatttgt gcagccttcc ggcagtgaag cacttcatcc ggaggggagg gcttgcgttt
     1621 ccgcagccgg ttacctttct ggtgggcgaa aacggcacag gtaaatctac actgctcgaa
     1681 gcaattgcgg tcgcctgtgg ctttaatccg gagggtggaa caaagaattt caccttttct
     1741 acatgcgcga ctcattcgga actttaccgc cacctgacca tcgtcagaca agcataccca
     1801 aaggatggct ttttcctgcg ggccgaaagc ttttataatt tggcaagcga tatggatgat
     1861 atggaccggg gattgccgga acgtatgaag ctgattaaaa gctacggcgg cacgtcgctt
     1921 catgctcaat cgcatggtga gagttttctc gctcttgcgg aaaaccgatt cggcggcaac
     1981 gggctttatc tgctcgacga acttggcccc aattgctaaa actggcgatt cgccacataa
     2041 caattggggc atattg
//
LOCUS       DATALY010000217         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_822079,
            whole genome shotgun sequence.
ACCESSION   DATALY010000217 DATALY010000000
VERSION     DATALY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822079"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VHR42_06210"
     CDS             complement(<1..1825)
                     /locus_tag="VHR42_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026803.1"
                     /translation="MKRFKSLKIKLVVCTVAIVCLTAVLSLVIGIYASYQSLTQNVRS
                     DLHSIGQTAEVAITNSLNNIKLNIQSVAKDDNIGNGSRADMTAYLDKQKKYFGYQSLS
                     VVNESGTIISDDTELDGKSVADQEYFKKAMQGETYISSTTYDINKKLCIIACTPLCNN
                     YKGVVLATLDPQTYSSIIKSIVVGKTGNVFMLDKQGTLIANIRPALVESRTNFIEKAK
                     TDLAYVTSAAVYKKMIAGKTGVDAYAYETGERICYYAPLKNTDGWSYGAVAPIAEMTS
                     TINYTIWGLGISSVICILLGILLAMVAANSIANPISRMSERLELLAKGDLHSEVPLVE
                     TKDEIGILSSSFADMVYTLNGYVSEISSVLGSLAAKDCTVDTQQHYRGDFAPIGTALK
                     KIVSNLNEIFGNIMRSTDQVATGAEQVSGASQALSRGATEQASSIEELSASITEIAQQ
                     VNRNAANAATANQLSLQAASQVMRSNEQMGQMVTAMSEISESSSEIGKIIKTIEDIAF
                     QTNILALNAAVEAARAGAAGKGFAVVADEVRNLASKSAEAAKNTTSLIESSVWSVENG
                     KKIADETAKSIHAVIEPAKKTTDLIGEISTASNEQATNLILS"
BASE COUNT          471 a          516 c          420 g          618 t
ORIGIN      
        1 cactcaaaat caagttggtt gcctgctcgt tggacgcggt agatatttca ccgatcaagt
       61 cggtcgtctt ctttgcaggt tcgatcaccg catgaatcga tttcgctgtt tcatccgcta
      121 tttttttgcc gttttcaacc gaccagaccg agctttcgat cagggaagtg gtattcttcg
      181 ccgcttccgc gctcttgctt gccaggttcc gcacttcatc cgcaacaacg gcaaagccct
      241 tgcccgccgc accggcgcgc gccgcttcca cagccgcatt caaagcgagg atattggtct
      301 ggaacgcgat atcctcgatc gtcttgatga tctttccgat ttcgctggag gattcgctga
      361 tctccgacat tgccgtaacc atttgcccca tctgttcatt gctgcgcatt acctgtgaag
      421 ccgcttggag ggacagttga ttggcggttg ccgcgttggc ggcgttcctg tttacctgct
      481 gtgcgatctc tgtaatggat gcggacagct cttcaatgga gcttgcctgt tccgtggcgc
      541 cccgcgaaag cgcctgcgac gcgccggaga cctgttccgc tccggtcgcc acctgatcgg
      601 tggaccgcat gatatttccg aaaatttcat tcaggttgga aacaatcttc ttcagcgcgg
      661 ttccgatggg cgcgaagtct cccctgtagt gctgctgggt atccaccgta caatcctttg
      721 cggcaaggct gcccaaaaca gaggatatct cgctcacata gccgtttaga gtatacacca
      781 tatcagcaaa agagcttgag agaatcccaa tttcatcttt tgtctctacc agcggcacct
      841 ccgaatgcag atcgcccttc gccagcagtt ccagcctttc actcatacgg gatatcgggt
      901 ttgctatgga attcgccgcc accatcgcta aaagaatacc gagtaaaata catataacgg
      961 atgatattcc gaggccccag attgtatagt taatcgtgga ggtcatttct gcaatcggcg
     1021 ccaccgcccc ataagaccat ccgtccgtat tttttaaagg cgcatagtag cagattcttt
     1081 cgcctgtttc ataagcataa gcgtctacgc cggtctttcc ggcaatcatc tttttataaa
     1141 ctgcggcgga agttacgtag gccaaatccg tcttggcctt ttcaataaaa tttgttctgc
     1201 tttctaccaa agccggccgg atgttcgcaa tcagagttcc ctgtttatcc agcatgaata
     1261 cgttcccggt tttccccacg acaatacttt ttataatact gctgtaagtc tgtggatcca
     1321 gcgttgccag caccacaccc ttataattgt tgcaaagcgg cgtacacgcg ataatacaca
     1381 gcttcttatt aatatcgtat gtagtagagg aaatataggt ttcaccctgc attgcttttt
     1441 taaaatattc ctgatctgca acgcttttcc cgtccaactc tgtatcgtcg ctgatgatag
     1501 taccgctctc attcacaacg cttaatgact gatacccaaa atactttttt tgcttatcaa
     1561 gataagcagt catgtctgct cggctgccgt tcccgatatt gtcgtccttt gcaacagact
     1621 ggatattcag ctttatatta ttcagagaat tggtgatagc gacctccgct gtttgcccta
     1681 tagaatgaag gtcacttctg acgttctgtg tcaggctttg ataacttgca taaatgccaa
     1741 tgaccagact tagtactgct gttaaacaga cgattgctac cgtgcataca accaacttga
     1801 ttttgagtga tttaaaccgt ttcatattat tttcctccac catttagttt aaatttttct
     1861 atttaaatcc ctctttttta ttctcataat gtattatcgt atgttttgaa cagaaaatta
     1921 gatttggtta ataaatattt taaagacttt taatagtaat attgacatat tcagtttcaa
     1981 gtgaactatc cggccatctc gataaaagtt tattttgacc gtttt
//
LOCUS       DATALY010000218         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_652149,
            whole genome shotgun sequence.
ACCESSION   DATALY010000218 DATALY010000000
VERSION     DATALY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652149"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VHR42_06215"
     CDS             complement(<1..656)
                     /locus_tag="VHR42_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026804.1"
                     /translation="MKIPQMDSRQDKNIDVIPNGGLNLSVLPNLIEDDQSPDMLNLWY
                     KDKAVSIRPALIKKIEQAYGKIIDVCPKDGRPFLIYDDPGALNDVSIYGVFIATKTAV
                     LYFDGTNITRVPTGFHDNGDKTYTNLYDDLNLSNAKFVESNKSYINGDVGNWQGNLAI
                     LIGDGHIFEVGLHNFGHLQTGLITEISIKEISYSKTLTTDYEWITPYVPTTYINLTPS
                     G"
     gene            complement(672..1070)
                     /locus_tag="VHR42_06220"
     CDS             complement(672..1070)
                     /locus_tag="VHR42_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026805.1"
                     /translation="MAVTGKNMVDQALRLIGHVNAQGAINENRESKYYAVALSYLTVL
                     QYELLNRENSSAVPVPVSDLNDNLLVSDETAYKVIPSGLAMYFSLIDRDTDNYNHFST
                     VYYNNLLPLIKPDNVVIEDYYGISSDSSFQ"
     gene            complement(1406..1789)
                     /locus_tag="VHR42_06225"
     CDS             complement(1406..1789)
                     /locus_tag="VHR42_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026806.1"
                     /translation="MAEFVKSLIGFSDDSKELVMTSAAGASDYFTLDNADHRVTLIVK
                     NANSTAGQDATVTLKAGNGILSTLGDEAFTVPMGKTYAIPLSRIDSARIKNLSGTNKG
                     KVMVSTTVAASGVVGSVSIGVISVA"
     gene            complement(1812..>2309)
                     /locus_tag="VHR42_06230"
     CDS             complement(1812..>2309)
                     /locus_tag="VHR42_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026807.1"
                     /translation="TIDATGTGNELTTKNVLSYLSDALAFIRSADVYDNGEIFFEVHP
                     YAFQKLQLAFTMLGQPNDQEFKNGYRGPLFGCQIYESNSIPVTDASGNDVAPGTAGAI
                     YHNIIRTNEAIAFAEQKAINFKAYEIEKGFGEGMKGFTLYGAKAVKPKELVCLKAKLG
                     TETDI"
BASE COUNT          586 a          535 c          511 g          677 t
ORIGIN      
        1 ccggaagggg tcagattgat ataggttgta ggaacatagg gagtaatcca ttcgtaatca
       61 gtagttaaag ttttggaata agaaatttcc ttaatggaga tttccgttat caatccggtc
      121 tgcaaatgtc caaagttgtg aagtcccact tcgaaaatat ggccgtcacc gatcaaaatt
      181 gctaaattac cctgccagtt tccaacgtca ccgttgatat atgatttatt tgattcgacg
      241 aatttcgcat tggataaatt cagatcatcg tataaattcg tataggtctt atctccgtta
      301 tcgtgaaatc cggttgggac tctggttata ttcgtcccat caaaatatag cactgctgtt
      361 ttcgttgcta taaaaacccc gtatattgaa acgtcattca aagcccccgg atcatcgtaa
      421 atcaggaacg gcctgccgtc tttcggacag acatcaatta tttttccgta tgcctgttca
      481 atctttttta tcagtgccgg acggatagaa accgccttgt ccttatacca caaattcagc
      541 atatccgggc tttgatcgtc ctcgatcagg tttggaagaa cggataaatt tagccctccg
      601 ttggggatta catcaatatt cttatcctga cgggagtcca tctgtggaat tttcatcaaa
      661 tcccctccaa ctcattgaaa acttgaatcc gacgatattc cgtaatagtc ctcgatcaca
      721 acattgtctg gcttgataag cggcagcagg ttgttgtaat agaccgtaga aaagtgattg
      781 taattatccg tgtccctgtc tatgagggaa aaatacattg ccagccccga aggtatcacc
      841 ttgtatgccg tttcatccga tacaagaaga ttgtcattta gatcgctgac cggcacagga
      901 acggcgctgg aattttctct gttaagaagt tcatactgca aaactgtcag atacgaaaga
      961 gcaaccgcat aatattttga ttccctgttt tcgttgattg caccctgcgc gttaacgtga
     1021 ccgattagtc ttagggcctg gtcgaccatg tttttccccg ttaccgccat tttgcaccgc
     1081 tccttttcta acgcctgttt gggcgtgtgg tgtccgtgtg ggcgcgtttt tacttacttc
     1141 tgatgtatgg ttatgggtat caggaatttc cgtgataacc tcaaattttc gctttaaagt
     1201 attaatgaat ctatcggtat agggaacggt taaaattccg ttctcgtccg accttccgaa
     1261 ttggtgaatt aatattcttc cgaactgtga aaaactatga taaaaattag tatttgggat
     1321 cattgccttg aaccgcatag aagatacctc ccgtttggta ttcgataaag gagaaggggg
     1381 cgggatcacc gcccctgata aacaatcaag caactgaaat aacgccgatg gatacgcttc
     1441 cgaccacgcc ggacgccgcc accgtagtgg ataccattac ctttcccttg tttgtgccgg
     1501 aaaggttctt gattctcgcg gaatcaattc ttgacagagg aatcgcatat gtttttccca
     1561 tcgggacggt aaatgcctcg tctcccagag tagacaggat tccgttacct gctttcagcg
     1621 ttaccgtcgc gtcctgcccg gcggtagagt tggcgttctt aacaatcagc gtcacacgat
     1681 ggtcggcatt gtcgagcgtg aaataatcag aggccccggc agccgaggtc attacaagtt
     1741 ctttgctatc gtcggaaaat ccgatcaggg attttacaaa ttcagccata ataaatcact
     1801 gttcctttcg tttatatatc agtttcagtg ccaagttttg ctttcaggca aaccagttct
     1861 ttgggcttca cagcttttgc accatagagg gtgaaaccct tcataccttc tccaaagccc
     1921 ttttcaatct cataggcttt gaagttgatt gccttttgtt cggcaaaggc gatagcctcg
     1981 ttggtacgaa taatattgtg atagattgcc ccagcggttc cgggcgcgac atcgtttccg
     2041 gaagcgtccg tgaccggtat ggaattgctc tcataaatct ggcatccgaa cagcgggccg
     2101 cgataaccgt tcttgaattc ctgatcgttc ggctgcccca gcatggtaaa cgcaagctgc
     2161 agtttctgga acgcatacgg atgaacctca aagaagattt caccgttgtc gtagacgtcg
     2221 gctgaacgaa taaacgccag tgcatcactc agataggaaa gcacgttttt ggtggtaagc
     2281 tcattcccgg tcccggtcgc atcaatggt
//
LOCUS       DATALY010000219         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_652234,
            whole genome shotgun sequence.
ACCESSION   DATALY010000219 DATALY010000000
VERSION     DATALY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652234"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VHR42_06235"
     CDS             <1..434
                     /locus_tag="VHR42_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX3026808.1"
                     /translation="EGLPLIRYRTRDRSRLAIEKCECGRTSARMFKVTGRTDDMLIIR
                     GVNVFPSQIEGVLLQIGGTTPNYQIIVNRVDNLDQLEIRVEMTSEMFSDEVRRIEDVE
                     VKITKELYSTLGINAKVRMVEPKTIARSEGKAKRVIDNRNY"
     gene            454..882
                     /locus_tag="VHR42_06240"
     CDS             454..882
                     /locus_tag="VHR42_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX3026809.1"
                     /translation="MVKQISVFLENKEGRLADVTKTLGDHGISIRALCIADTTDFGIL
                     RLIVNNPDLAYKVLKEAEFAVSLKDVMAIAIQDQPGSLSKALALLQGNNVVIEYMYAY
                     LGRIENKAIVIMKVSEYDRAVSILNDGGFEVITAEDVYNF"
     gene            complement(1069..>1647)
                     /locus_tag="VHR42_06245"
     CDS             complement(1069..>1647)
                     /locus_tag="VHR42_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026810.1"
                     /translation="ILALNAAVEAARAGQHGKGFAVVAEEVRNLAARSANAAKETTAL
                     IEGSIAKVESGTKIANETADALGKIVDVVSKAAELVGDITNASNDQATSIAQVSQGIT
                     QISDVTQTNSATSEQSAAASEELSGQAEVLQQMVQKFRLNTGAEPEKAVTKSYSSNRG
                     FEKSSHLQTETKKKAAAAKPTINLMREDFGKY"
BASE COUNT          399 a          377 c          439 g          432 t
ORIGIN      
        1 aagagggcct gccgcttatt cgttaccgca ccagagacag atcaaggctg gcaattgaaa
       61 aatgcgaatg cggaagaact tcagcgcgta tgtttaaggt gacaggcaga accgatgata
      121 tgctgattat ccgcggtgtc aatgttttcc cgtcccagat cgagggagtg ctgctccaga
      181 tcggcggtac tacgccgaat tatcagatta tcgtcaaccg tgtcgacaat cttgaccagt
      241 tggaaatccg ggttgaaatg acatccgaga tgttctctga cgaggttcgg agaattgagg
      301 atgtggaagt aaagatcacc aaagagcttt acagcaccct tggaattaat gcgaaggtcc
      361 ggatggttga gcccaagacg attgcccgca gcgagggtaa ggccaaacgt gtcatagaca
      421 atcgaaacta ttaagaaaaa ggggtggcgt tctatggtta agcagatttc ggtttttctg
      481 gaaaataaag agggcagact ggccgacgtg acaaagacgc tgggagacca cggaattagc
      541 atccgggcgc tctgcatcgc ggatacgacg gatttcggaa tccttcgtct gattgttaac
      601 aatccggatc tggcctacaa ggtgctgaaa gaggctgaat ttgccgtcag tctgaaggac
      661 gttatggcaa ttgccattca ggatcagccg ggctctcttt caaaagcgtt ggccctgctt
      721 cagggcaata acgtcgtgat cgagtatatg tatgcgtatc ttggacggat cgaaaacaag
      781 gcgatcgtca ttatgaaggt ttcggaatac gacagggcgg tttctatttt gaatgatggt
      841 ggttttgaag tcatcaccgc ggaagatgtt tacaacttct aacgtctgaa tgatatgtgc
      901 ctgaaattaa gagcatggtt tctgtcatgg aaaccatgct cttttctcct atgcgtggaa
      961 ataaaccctg gccgatggca caatcctgca aaaaggcaaa ataacaaggg gggacaggcc
     1021 tgcaaacctg tcccccttgc gatagataaa actacaattt taaaagattt aatatttgcc
     1081 gaaatcttcc ctcatcagat taatggtcgg ctttgcagca gctgctttct ttttcgtttc
     1141 ggtttgcaaa tggctgcttt tttcaaaacc acggtttgac gaataggatt ttgtcaccgc
     1201 tttttccggt tccgcaccgg tattcaggcg gaatttttgg accatctgct gcaggacttc
     1261 agcctgaccg gacagttcct cactggctgc ggcgctttgt tcggaagtgg cggaattggt
     1321 ctgggttaca tccgatatct gtgtaattcc ctgactgacc tgcgcaatgc tggtagcctg
     1381 atcgttggaa gcgttggtaa tgtcgccgac cagctcagca gctttggata cgacgtcgac
     1441 aattttcccg agggcgtccg ctgtttcatt ggcaatcttt gtgccgcttt caaccttagc
     1501 gattgagccc tcgatcagag cggtcgtttc cttggcggcg tttgcgcttc tggcggcgag
     1561 gttccggact tcttccgcaa cgacggcaaa acctttgccg tgctgcccgg ctcttgcggc
     1621 ttcaacagcc gcgttcagcg caagaat
//
LOCUS       DATALY010000220         7404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_393043,
            whole genome shotgun sequence.
ACCESSION   DATALY010000220 DATALY010000000
VERSION     DATALY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393043"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(504..2105)
                     /locus_tag="VHR42_06250"
     CDS             complement(504..2105)
                     /locus_tag="VHR42_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX3026811.1"
                     /translation="MSMSAAKFQKLFVFFLIAVMTVSVLSGSKIYDVSAAVNPKFGVS
                     TSTSLTSRTATVSASVLNVRASNSTGSSVIGKLARGASVYILADSKGWYKISYNGKTG
                     WVSGQYLKNIRAVNVKSDKTTSAQSRSSSAPKSTSSAAEKTSSSPAPASSSASSSSAP
                     TSSSVTSSSSASSPMNAKTVPTSSLPSKISAGYYTSWSAYSGYTPSQINALKLNVVHY
                     AFAGIGSNLKIAVGDSSVDYTNFTKLNALKKSYPALKTLISVGGWDGSSRFSDMALTD
                     STRRAFAGSCVSFIKQYGFDGIDIDWEYPVSGGSPGRPEDKGNFTLLMKSIREALTAQ
                     GISDGKYYYLTFAGGASNGYINNVQLSLLSGYVDYAVDMTYDLHGPWDAYTDFNAPLY
                     TPGGVSPQYKISVDSSLRSWRSAGFPSGKLVMGVPFYGYVYSGVNSANNGLYQKFTSA
                     KSIGYDSIVSSYLNNPLYTKLYQSTGQVPYLFGNGKFISYDDAASIAAKTRYVSANGL
                     RGVSAWELSYDRSSVLLGAVSQNLR"
     regulatory      complement(2179..2263)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2512..3066)
                     /locus_tag="VHR42_06255"
     CDS             complement(2512..3066)
                     /locus_tag="VHR42_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026812.1"
                     /translation="MFQSEKKLKLTNANLNDYNFDPHPNVSGHNLIYQAHRSVPIRFS
                     RNALKILGADQVTIPYSLTPGVTACAKYSAQSLLSCLAPDDTALRSVYSIADSGSTGA
                     VISPSGELTVKNPGTVRIKAVLSSSKSNLSVEGVKTVTVKRENPPKRSFLDGKLPMIA
                     AIFAIAVVAVILLVRKFKKKKRKN"
     gene            complement(3053..3745)
                     /locus_tag="VHR42_06260"
     CDS             complement(3053..3745)
                     /locus_tag="VHR42_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX3026813.1"
                     /translation="MQTKKTKRFLSVFLSMLIVSSVFFGAKADAIKQRAITYTALGDS
                     ISTGYKLSNTSKDYVNLFGKYLNTVPVNLGKNGLDSAGLLRKLNQDQTVIGQIKKSDV
                     ITVSMGGNDMLVIFSSLNLDLTSPLSLVNAVSLLQGNTLHRKLTGGVESFSKNWPEIV
                     ARLKNLAPDANLVVTTLINPYQGIVLKVPLLLNFDLGAFSEPYIRQINSVIKKRRKRE
                     LSGGRFLFDVSE"
     gene            complement(3766..3918)
                     /locus_tag="VHR42_06265"
     CDS             complement(3766..3918)
                     /locus_tag="VHR42_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026814.1"
                     /translation="MDAILSGGTISRLSEKTQKTLRDFDLDEFLPWVLARNFRAILNH
                     QTTSKT"
     gene            complement(3929..4702)
                     /locus_tag="VHR42_06270"
     CDS             complement(3929..4702)
                     /locus_tag="VHR42_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026815.1"
                     /translation="MTAYQVIKDMKNAGFRATIVPAESVRDIRVEMESLIVSGALDAG
                     IVAKYGRHFQNDFISRYPAARSIILVAAPCPVHRLTFSYHGTCCEAVIPPGYARMGLK
                     SAVEHQVSGLLRQSGYSCEKLEGVPLKLIAVKSGLCLYGRNNITYCEGLGSYIRLFGF
                     VSDIQAPQDALWRPAERMPECADCGGVYPRLPQRMYWRSFKNHPCRALPDLFERKWRG
                     LSRLAESRLTQRGYRLHALSESLSEKSRASENADNGECV"
     gene            complement(4764..5084)
                     /locus_tag="VHR42_06275"
     CDS             complement(4764..5084)
                     /locus_tag="VHR42_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative heavy metal-binding protein"
                     /protein_id="HEX3026816.1"
                     /translation="MIITTTPSIEGKQIVEYRGVVFGEVISGVNFMKDIAAGLSNIFG
                     GRSGTYEEELIQARENAMNEMEQRAARLGANAVVGVDIDYEVLGSDNGMLMVTASGTA
                     VRYE"
     gene            5423..6337
                     /gene="pyrB"
                     /locus_tag="VHR42_06280"
     CDS             5423..6337
                     /gene="pyrB"
                     /locus_tag="VHR42_06280"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX3026817.1"
                     /translation="MLQTRHLISPDDISVKEIDEIIELAQKIIKTPVSYADYCKGQIL
                     ATLFYEPSTRTRFSFEAAMLRLGGNVMGFSDPDSISAKKGESIIDTIKVISIYADIAV
                     MRHPKEGAPYLASKFASIPVINAGDGGHQHPTQTLTDLLTIRSEIGSLSGYTIGLCGD
                     LKFGRTVHSLVKALSRYDNIRFVFISPKELSAPQYVIDYLKPGQYSIVDDLEEALPEL
                     DILYMTRIQKERFLNEEEYLKLKGIYVLDRKKMTFAKEKLVVMHPLPRVDEISMEVDN
                     DPRALYFKQAQYGMYVRMALILKLLGKA"
     gene            6394..6825
                     /locus_tag="VHR42_06285"
     CDS             6394..6825
                     /locus_tag="VHR42_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEX3026818.1"
                     /translation="MLSIKQIDRGLVIDHIEKGFGEQILKQLNLNSEFTVALLRNVPS
                     RKSGKKDIIKIDNISIDDVDLNVLGLIDPGVTINVISDGKLAEKIKLTLPDKVIGIFK
                     CNNPRCVTTAEQVETTFYLSDEATREYRCEYCDARVTLRKK"
     gene            complement(6884..7345)
                     /locus_tag="VHR42_06290"
     CDS             complement(6884..7345)
                     /locus_tag="VHR42_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX3026819.1"
                     /translation="MKAVRHSKILELITTKEIKTQEELTKELRKRGMKVTQATVSRDI
                     KQMGLIKVPAGNGEHKYVPASPYDDFLIDKLVSILSASIVSLDIVEKIIVVKTISGAA
                     SAAAEAIDSIKYDGIVGTLAGNNSIFILARNEEKAKGAYRYIENLMKIKNS"
BASE COUNT         1905 a         1852 c         1695 g         1952 t
ORIGIN      
        1 atcggattat caaaggtcag gatctgaaaa cagacgccat tccgttcttc atagcgatga
       61 agtgcggcag caagcttctc gatgctgatc ggttcatcat cacacagggc aatacgaatt
      121 gtcctaaagc ttaaaatgtc acttcccgtt tcatgcataa tctgtcacct cccctgcagc
      181 acttaatcac ccatatttta aactgttcat tttatatttt caactttaat cttttctgcc
      241 ggtcataatc accctgtttt ccgttcaatt cgacccgaca cgataccagg tttcaccaaa
      301 tatttcattt attccgcgta atcttaacct tcttgataac cggcaaagcg gcaagaagac
      361 attcgtttaa tctgtgaaaa ttgaaaaagg ggaatcgccg gaattccata aaataagcaa
      421 gccggcaaac gatggaatat cgtctgccgg cttctgagat cggacatctg atgaccgcga
      481 attgattcat tccatgaaat cattcagcgg aggttttgag aaactgcgcc aagaagcacg
      541 gaggatctgt cataactcag ttcccaggcg gatactcctc tgagcccatt ggccgagaca
      601 tacctcgtct tcgcagcaat ggatgcagcg tcatcataac tgataaactt gccgtttcca
      661 aacagatagg gcacctgacc cgttgactga taaagctttg tatacagcgg gttattcaga
      721 taagaggaaa cgatgctgtc atagccgatt gatttggcgc tcgtaaattt ctggtaaagc
      781 ccgttgttgg cggaattgac gccggaataa acgtaaccgt aaaacggaac ccccatcacc
      841 agcttgcccg aaggaaatcc tgcgctccgc caggaacgaa gcgaagaatc aacgctgatt
      901 ttatattggg gtgaaacacc gcccggcgta tagaggggcg cgttgaaatc agtgtaggca
      961 tcccacggcc catggagatc ataggtcatg tccacggcat agtccacata cccggaaagc
     1021 aaactcaact gcacattatt tatgtagccg ttggacgctc ctcccgcaaa tgtaaggtaa
     1081 taatattttc cgtctgaaat accctgagcg gtcagagctt cacgaatcga tttcatcagc
     1141 agtgtaaagt ttcctttgtc ttccggcctg cccgggcttc cgccggaaac gggatattcc
     1201 cagtcgatat cgatcccgtc aaagccgtat tgtttgataa aactgacaca gctgccggca
     1261 aatgcccttc gtgtggaatc ggtcagcgcc atatcggaaa accgtgaaga tccatcccat
     1321 cctccgactg aaatcagagt ttttaaagcg ggatagcttt ttttgagcgc atttagtttt
     1381 gtgaaattgg tgtaatcaac ggaagaatcc ccaactgcga ttttcagatt gcttccgatt
     1441 ccggcgaagg cgtaatgcac gacattcagc ttcaacgcgt taatttggga aggagtatac
     1501 ccactgtatg ccgaccagct cgtataataa cccgcggata tttttgacgg cagactggat
     1561 gtcgggacgg tctttgcatt cattggcgag gacgccgaag aggacgatgt caccgacgat
     1621 gaggtaggtg ccgaagagga cgatgccgac gatgaggcag gcgccgggga tgaagatgtt
     1681 ttttctgcgg ccgagcttgt cgacttcggc gcggaggatg atcttgactg tgcagacgtc
     1741 gtcttgtccg attttacgtt taccgctcgg atatttttga gatattgccc tgaaacccat
     1801 cctgttttgc cattgtaact gattttgtac catcccttgc tgtcggccaa aatataaacg
     1861 gaggctcccc gggcaagctt tccgataacg gatgatcctg tgctgttcga tgcccttacg
     1921 tttaaaacac ttgccgacac tgttgccgtc ctgcttgtca gactcgtgga tgtcgaaacg
     1981 ccgaacttcg gatttactgc cgctgatacg tcataaattt ttgaaccgga tagcactgaa
     2041 accgtcataa cagcaatgag aaaaaaaaca aatagtttct ggaattttgc cgctgacatt
     2101 gacatgggca tccccctttt tcagttgcag acatcaaaac ggcaacccat acggctgccg
     2161 tttatgacgt ttcatcatta cggcagctgg ctgccatagg ctgacgcctt taggcccttt
     2221 agctttgcgt cgctgccttt cgacagttct gcctttttct tgtaatacca ttatattcta
     2281 taatattttt agagacaatg aataaattat ggtatttacg attgttaaaa agtgtttatt
     2341 attacaatta aatggaatct tttatacaaa agcaccatca cttgctcctg ttctcaattt
     2401 gccaagcagc catcgaaaag gtaaaacaat cgattcaaac agaaaatcag ccggaggtta
     2461 aaccttcggc tgattttcag cggtttctaa acttcccggc tggtggccgg atcagttttt
     2521 tctcttcttc tttttgaatt tccgcacaag aaggatcacc gcgaccaccg cgattgcaaa
     2581 aatggcggcg atcatcggaa gtttaccgtc aaggaagctc cgtttgggtg ggttctccct
     2641 tttgaccgtt actgttttca ccccttccac ggagagattt gattttgaag aggataaaac
     2701 cgctttgatt cggactgttc cgggattctt gaccgtcagt tctcccgatg ggctgatgac
     2761 cgctcctgtt gagccggaat ccgcaatgga gtaaacggag cggagagccg tgtcgtccgg
     2821 agcaaggcag ctcagcaggg attgagcgct gtattttgcg caagcggtta ccccgggagt
     2881 cagggaatat ggaatcgtta cttgatcggc acccaggatt ttcagcgcat tccgggaaaa
     2941 ccggattggt accgaacggt gagcctgata aatcaaatta tgtccgctga cattcggatg
     3001 agggtcaaag ttgtaatcat tgagatttgc attggtcagt ttgagctttt tttcactctg
     3061 aaacatcgaa taggaatcgg ccacctgata attccctttt gcgtcttttt ttgatcacgc
     3121 tgttgatctg cctgatatag ggttcggaaa aggcgcccag atcaaaattg agcagaagcg
     3181 gaacttttag gacgattccc tgataaggat taatgagcgt cgtgacaacg aggttggcat
     3241 ccggcgcaag atttttaagc cgggcgacaa tctccggcca gtttttgctg aaactttcga
     3301 caccgcccgt aagcttccgg tgcagggtat tgccctgaag aagcgagacc gcgttaacaa
     3361 gcgacagagg ggatgtcagg tccaaattca ggcttgaaaa tatcaccagc atatcgttcc
     3421 cgcccatgga aacggtgatg acgtccgatt ttttaatttg cccgatcacc gtttgatcct
     3481 gattgagttt tcggagcaag ccggcgctgt caagcccgtt ttttccaaga ttgaccggca
     3541 ccgtattcag atatttccca aaaagattca cgtagtcttt ggacgtattg ctaagcttgt
     3601 atccggtgga gatcgaatct ccgagagcgg tataagtgat cgccctctgt ttgattgcgt
     3661 ctgctttcgc gccaaaaaag acggatgaaa cgatcagcat ggaaaggaac acggaaagaa
     3721 atcgtttggt ttttttggtt tgcatgtttc aggcccacct atattttaag tcttcgatgt
     3781 cgtttgatga tttaagatcg cgcggaaatt gcgcgcaagc acccacggca ggaactcatc
     3841 gagatcaaaa tcccgaagcg ttttctgcgt tttctccgaa aggcgcgaga ttgttccgcc
     3901 ggatagaatt gcatccactt ccgtctcttc aaacgcattc tccgttatct gcgttttcag
     3961 aagcccgcga tttttcggac aaactttctg acaaagcatg cagccgataa ccgcgttgtg
     4021 tgagtcggga ttcagccagt cgggaaagtc ccctccattt tcgttcaaat aagtcaggca
     4081 gcgctcggca cggatgattt ttgaatgatc gccaatacat ccgttggggc aaacgtggat
     4141 acacgccccc gcagtccgca cactccggca ttctttccgc cggacgccac aaggcatcct
     4201 gcggcgcctg aatatcggag acaaagccaa acagcctgat atagctgcca agcccttcgc
     4261 aataagtaat attgtttcgc ccataaaggc acaggccgct tttcaccgcg atcagtttca
     4321 gcggcacacc ctccagcttt tcgcaggaat atcccgattg acgcaggaga ccggacacct
     4381 gatgctcaac agcggatttc aggcccattc tcgcatatcc cggaggaatc accgcctcac
     4441 agcaggttcc gtgataggag aaagtcagcc gatgcaccgg gcagggagcc gcaacgagaa
     4501 taatcgaccg ggcggccgga taacgtgaaa tgaaatcatt ttgaaaatgc cttccatatt
     4561 ttgcgactat ccccgcatcg agagctccgg acacgatcag cgattccatt tcgacacgaa
     4621 tatcccgaac gctttcggcc ggcacaatcg tggcccgaaa gccggcgttt ttcatatcct
     4681 ttattacctg atacgcagtc ataaaaagcg cctcctcagc aaaactgccg tttgtctttt
     4741 tggcgggccc atttttgccc ggattactca taccggaccg cagtaccgga agccgtcacc
     4801 ataagcatac cgttatcaga cccgagcacc tcgtagtcaa tatccacgcc aaccacagca
     4861 tttgcaccga gccgggccgc cctctgctcc atctcattca tcgcattttc ccttgcctga
     4921 atcagctctt cctcataagt gccggaacgg cccccgaaaa tgttcgaaag acccgcggca
     4981 atatccttca tgaagttgac gcctgatatt acttccccaa ataccacgcc cctgtattcg
     5041 acaatctgtt tgccttcaat cgacggtgtt gttgtgataa tcatcgtttt cctcccggat
     5101 gttttttctt tttgaattcg ataccgcaag ggaccgtttt tcgcggcgca gcatggactg
     5161 aatattattt taagcctgcg gatcaaggaa gtcaacgcag gacaccgcca ttgctgtttt
     5221 cagacgctgc cgcggcggtt ctatttattt ttaaaattct tattgacaaa tgagcaaata
     5281 tctcttacac tattagaaaa caaatcttta acccggtcct gtgaggccgg aaaggttgca
     5341 ataacggact ctctttatgg gattattgcg tctttccgag ctgaggaaag gcgctatttt
     5401 tataaaaggg aggctgtttt atatgctcca gaccagacat ctcatcagcc cggatgacat
     5461 ttctgtaaaa gagattgacg aaattatcga gttggctcag aaaatcatta aaacacctgt
     5521 aagctacgcg gattactgca agggacaaat tcttgcgaca ctgttttacg agccgtcaac
     5581 aagaacacgt tttagcttcg aggcggctat gctccgcctg ggcggaaatg taatggggtt
     5641 ctcagacccc gattcaatct ccgcgaaaaa aggcgaatca atcatcgaca ccatcaaggt
     5701 catctcgatc tacgcggata ttgccgtcat gcgccatccg aaagaaggag ccccttatct
     5761 tgcctccaaa ttcgcctcta tcccggtcat caacgcgggt gacggcggac atcagcatcc
     5821 cacccagacg ctgaccgatc ttcttacaat ccggtcggaa ataggcagtc tgtcggggta
     5881 taccatcggc ctttgcggag acctgaaatt tggccggacg gttcattccc tggtcaaggc
     5941 gctctcccgc tacgacaaca tccggtttgt ttttatctcg cccaaagagc tcagcgcgcc
     6001 gcaatatgtc attgattatc tcaaacccgg tcagtattcg attgtggacg atctggaaga
     6061 ggctcttccc gaacttgata ttctgtatat gacccggatt cagaaagaac gttttctgaa
     6121 cgaagaagag tatctcaagc tcaagggtat ctatgtcctt gaccggaaaa agatgacttt
     6181 cgccaaagaa aaactggttg tcatgcatcc gcttccgcgt gtggatgaaa tctccatgga
     6241 agtggataat gacccaaggg cactctactt taaacaggcg caatacggca tgtatgtccg
     6301 gatggctctg atcttaaagc tgttgggcaa agcatgagcc ggacaattct taagaaatcc
     6361 ggcccaaaaa taacattgcg ggaggcatgt gatatgttga gcataaagca gatcgacaga
     6421 ggccttgtca ttgatcatat cgagaaggga ttcggcgaac aaattctaaa gcagctgaat
     6481 ttgaacagcg aatttaccgt tgcgctgctg cgcaacgttc cctccagaaa atccgggaag
     6541 aaagatatta taaaaatcga caatatctcc atcgacgacg tggatctgaa tgtgctgggg
     6601 ctcattgacc cgggcgtaac gatcaacgtc atatccgacg gaaaactcgc ggaaaagatt
     6661 aagctgaccc tgcccgacaa ggtaattggg atctttaaat gcaacaatcc ccgctgcgtg
     6721 accaccgcgg agcaggtaga gacaaccttt tatctttccg atgaggcaac gcgggaatat
     6781 cgctgtgagt attgcgacgc ccgtgtaacg cttagaaaaa aataaaggcg gaaactctct
     6841 tccgccgggc cgtaaatgtt ccggccctat caattccggt cttctaacta ttttttattt
     6901 tcatgaggtt ctcaatatac cggtatgccc ccttggcttt ttcctcattt cgagccagaa
     6961 taaaaatgga gttgttcccg gccaaagttc ctacaattcc atcatatttt atgctgtcga
     7021 tcgcttcggc agcggccgac gcagcccctg atatggtttt aacgactatg atcttctcaa
     7081 caatatcaag actgacaatg gaagcggata aaatcgacac cagtttatca ataagaaagt
     7141 cgtcataagg agacgcgggc acatatttat gttccccatt cccggcagga accttgatca
     7201 gtcccatttg tttgatatca cgcgataccg tcgcctgagt aaccttcatc cctctctttc
     7261 ttaattcttt cgtcagttct tcctgtgtct taatctcctt tgtcgtgatc aactcaagaa
     7321 tcttcgaatg tcgtacagcc ttcatcattc tccactttct tatctttatc ggaactattt
     7381 ctactgctga tcgcagacat aaac
//
LOCUS       DATALY010000221         4144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_653742,
            whole genome shotgun sequence.
ACCESSION   DATALY010000221 DATALY010000000
VERSION     DATALY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653742"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VHR42_06295"
     CDS             <1..938
                     /locus_tag="VHR42_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026820.1"
                     /translation="DISRFLDEIARGNLTVESECEYVGEFTRIHTSMETILSSLNQTI
                     TQIRLAAEQVSSGSNQMSDGAQALSQGATEQASAIEELSATISDVSNQLKNNASDSEA
                     ASVKVGELHLEMKQSTDQMQNMIASMQQINDTSNQIGKIIKTIDDIAFQTNILALNAA
                     VEAARAGAAGKGFAVVAEEVRNLASKSAEAVKVTTTLITSSMKAVEDGAEIADQTAKS
                     LTKASEKTEDVTSLVVRISDATNKQSNSIAQITQGVDQVASVVQTNSATAEESAASSE
                     ELSGQAEMLRSLVSEFRLMETAPAEKPAESTEPEV"
     gene            complement(1010..2896)
                     /locus_tag="VHR42_06300"
     CDS             complement(1010..2896)
                     /locus_tag="VHR42_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX3026821.1"
                     /translation="MIAIAVLLNILLERIDRRNEEVLKLNKQITNHMEMMEKSEELSN
                     MGTWRLSLKTQKSIWSNGLYQIFGITPNSITPSLEKRLELSHPDDREMIRKAIANSID
                     TKSSVRIDSRIIRPDGSTRWISSIGYTEYNETGKPETYIGSFIDITDRKAYEQELYHE
                     KEKFRITITSIGDGVIATDEKGNITLLNEQAEKLTGWEQKDATGKPFDEVFNMISEET
                     REKRESPIPKVIKSGLILGLANHTALISKYGAEYSIADSAAPIKDRHGIIKGVVIVFR
                     DVTEEKKRQEQISYISYHDSLTGLYNRRFFEEQLREIDTAENLPISIIMGDVNGLKVT
                     NDAFGHAEGDRLLQRASDAIRAACRSDDIIARWGGDEFVVLLPKTEKSAAEDIVERIM
                     EIEAQMKVGPINVSVSFGCGVKKEESDNILKALKNAEDHMYENKLSESEGMKGNLINS
                     ILNTLYEKNPDEVDHFNRVGDLSQKIGKALHLPKEDLSKLKSIGRFHDIGKIAVSNSI
                     LKKQGPLTPQEWDEIKRHPDIGYKILKSSRETVHLSEYVLSHHERYDGKGYPSGLKGE
                     EIPLLSRIVSVADSFDAMTNDRSYRRALSATDALDELVKNKETQFDPTVVDAFVELLK
                     VKAE"
     gene            complement(3456..3541)
                     /locus_tag="VHR42_06305"
     tRNA            complement(3456..3541)
                     /locus_tag="VHR42_06305"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3505..3507),aa:Tyr,seq:gta)
     gene            complement(3746..>4144)
                     /locus_tag="VHR42_06310"
     CDS             complement(3746..>4144)
                     /locus_tag="VHR42_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026822.1"
                     /translation="LHKWLSVENLDLEQKVFSKINKEDTPILWFGGLSSIVDKSNNMP
                     AKIQSEFHEKSQIYLAGRLSGSTGQEKLQANWHFPNGTIKTDDFTLNGNKSYCKNYST
                     SEIGDGLGEVSIIMKETGKVLVVYPFSIIK"
BASE COUNT         1026 a          943 c          940 g         1235 t
ORIGIN      
        1 cggatatttc cagattcctg gatgaaatcg caagggggaa tcttaccgtt gagagtgaat
       61 gcgagtacgt cggggaattt acgcggattc acacctcgat ggaaacgatt ctgtccagtc
      121 tgaatcagac cataacgcag atccgacttg ccgcggagca ggtcagcagc ggctcaaatc
      181 agatgtccga cggtgcgcag gcgctttcac agggagctac ggagcaggcc agcgccattg
      241 aagagctctc tgcgaccatc agcgacgttt ccaaccagtt gaagaataat gcttcggatt
      301 ccgaggcggc aagcgtcaag gtcggtgagc tgcacttgga aatgaaacag agcactgatc
      361 aaatgcagaa tatgattgcc tctatgcagc agatcaacga tacttcaaat cagatcggga
      421 aaattatcaa aacgattgac gatattgcat tccagaccaa catcctcgcg ttgaacgcgg
      481 cggtcgaagc agccagggcg ggcgcggcag ggaagggctt tgccgtggtc gcggaagaag
      541 tccgtaacct tgcaagcaag agcgcagaag cggttaaggt tacaacaaca ctgattacca
      601 gctccatgaa agcggtggaa gacggagccg agattgcgga tcagacggcg aaatcgctta
      661 caaaggcctc cgaaaagacg gaggatgtaa cgagccttgt ggttcgaatt tccgatgcga
      721 ccaacaagca gtcgaattcc atcgcccaga ttacgcaggg tgtcgatcag gtggcttcgg
      781 tggttcagac caactccgct acggccgaag agagcgcggc gtcttccgag gagctttccg
      841 ggcaggctga gatgctccgt tctctggtga gcgaattccg tctgatggaa acagctccgg
      901 cagaaaaacc tgctgaaagc acggaaccag aagtttaatc tgtttacaat catcattttt
      961 tctcataaga ggggcccgca gaattacaag ttctgcggac ccctttgttt cactctgctt
     1021 taactttcaa cagttccacg aacgcgtcaa cgacggtcgg gtcaaattgg gtttctttgt
     1081 ttttcaccag ctcgtccaac gcgtcggttg cggataaggc gcggcgatag ctccgatcgt
     1141 tcgtcatggc gtcaaatgaa tccgcgaccg agacaattct ggagagcaga ggaatttctt
     1201 cccccttgag tcctgatggg taaccttttc cgtcgtaccg ttcatggtgc gaaagaacat
     1261 attcggataa atggaccgtt tcccgggagg atttcaatat tttatagccg atgtccggat
     1321 gccgcttgat ttcatcccat tcctgcggag taagcggccc ctgctttttc agaatcgaat
     1381 tgcttacggc gatttttcct atatcgtgaa agcggccgat tgattttagc tttgatagat
     1441 cttcctttgg caaatgaagt gccttcccaa ttttttgaga gaggtctcct acccgattga
     1501 agtgatccac ttcatccgga tttttttcat acagcgtatt tagaatggag ttaatgagat
     1561 tccctttcat cccttcgctt tcgcttaatt tattttcata catgtggtct tccgcatttt
     1621 tcaaagcttt tagaatatta tcgctttctt cttttttgac cccacatccg aaggagacgg
     1681 aaacgttgat cggcccaacc ttcatttgag cttcaatttc catgattcgc tcaacaatat
     1741 cttccgctgc cgatttctct gtttttggaa gaagaacaac aaactcgtct ccgccccacc
     1801 gtgcgataat gtcgtcggag cggcaggcag cccgaatcgc atcggaagcc ctttgcagaa
     1861 ggcgatcgcc ttccgcgtgc ccgaatgcgt cattggtaac ctttagcccg ttgacatcgc
     1921 ccataatgat tgaaatcggc aggttttccg cggtgtcgat ttcccggagc tgttcctcaa
     1981 aaaatctgcg gttataaaga ccggtcaggc tgtcatggta acttatgtag ctgatttgtt
     2041 cctgtctctt cttttcttcg gtgacatccc ggaaaacaat cacgacgccc ttgatgattc
     2101 cgtggcggtc tttgatcggt gcggcgctgt cggcgatcga atattccgcg ccgtattttg
     2161 agatgagtgc cgtgtggttt gcaagtccca ggattaaccc gctctttatg acttttggga
     2221 tcggagattc ccgcttctcc cttgtttctt cgctgatcat attaaaaacc tcgtcaaaag
     2281 gttttccggt cgcgtctttt tgttcccaac cggtaagctt ttccgcctgt tcattcaata
     2341 acgtgatgtt tcctttttca tccgtggcga tcacaccatc tccgattgaa gtgattgtga
     2401 tccgaaactt ttccttttca tggtaaagct cctgctcata ggcctttcga tccgtaatgt
     2461 caataaagga tccgatatag gtttcgggtt taccggtttc attgtattcc gtataaccga
     2521 tggaggaaat ccatcgggtg cttccgtccg gccggataat tctgctgtcg attcttacac
     2581 tgctttttgt atcgattgaa tttgcgattg cttttcgtat catttcccga tcgtcgggat
     2641 gcgacagttc caagcgtttt tccaaggacg gtgttatgct gttcggcgtt attccaaaaa
     2701 tttggtataa gccgttggac catatgcttt tttgagtctt taaactcagt ctccaggtgc
     2761 ccatgttgct gagttcttcg gacttttcca tcatttccat atggttggta atctgtttat
     2821 tcaattttaa gacttcttca ttccgccggt cgattctttc caataggata ttaagaagca
     2881 cggcgattgc aatcattacc accaaacgcg cgaattcttc cggcagattt tctttccgat
     2941 aaaatgagta gatataaatg tgaaccgcgg ctatgattcc cgaaaaaaca ataccttttt
     3001 tttctgtacc aaaaacatgc aatgattaag gggataaaga acagattctg atagatgatg
     3061 tagatattgt ttgacaagca gcatacggaa gcagcgaata ttgcgcctgt gatcgacagc
     3121 agcagaagaa gcttattttt aggcgtagct ttcatattct tattctcatt tcatttttct
     3181 atccgtggag ttcttccatt ataacaggct tattggaaac attatactta ttctcaaaaa
     3241 aatgacttgt ttttttcgaa ttctgtatta gttatataaa aaggatggcc gatatcattt
     3301 catacgatac cgaccatcct tttttaggaa aaaagtgggg aaagtgcgag aagcccggcc
     3361 taaaaacgtg tccccggcac gttttcttga tggccttccg aatccgtcaa tgtgctgaat
     3421 aaaaaatcag acaagtcaaa agacttgtct gattttggtg gaggaggatg gattcggacc
     3481 atcgaagccg taaagcaaca gatttacagt ctgccccatt tgaccgctct ggaactcctc
     3541 catgatatgc aattttaagg gaataattgg agctggcaga cggacttgaa cccccgacct
     3601 gctgattaca aaagtctaat aaagaattat gttgtatttt taggagttag gcgagcccca
     3661 ttgatggctt gtacgctgga tttttcgata ggttgcattt ttatcaaatt ttgagcggat
     3721 accaatttag ataccttgga taccactatt ttattatact aaaaggatag actacaagaa
     3781 ctttccccgt ttctttcatt ataattgata cttctcccaa accgtcgcct atttccgatg
     3841 tggaataatt cttgcaatat gatttatttc catttagtgt aaagtcatca gtctttatgg
     3901 ttccgtttgg gaagtgccaa tttgcttgaa gcttttcttg tcctgttgat ccgcttaatc
     3961 ttccagctag gtatatttga cttttctcat ggaattctga ttgtattttt gcaggcatat
     4021 tattggattt gtcaacaatc gaacttaacc cgccaaacca aagaattggc gtgtcttctt
     4081 tgttgatttt ggaaaatacc ttctgctcta aatctaagtt ttcgacagaa agccatttat
     4141 gtag
//
LOCUS       DATALY010000222         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_737052,
            whole genome shotgun sequence.
ACCESSION   DATALY010000222 DATALY010000000
VERSION     DATALY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737052"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1530
                     /gene="ypeB"
                     /locus_tag="VHR42_06315"
     CDS             184..1530
                     /gene="ypeB"
                     /locus_tag="VHR42_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HEX3026823.1"
                     /translation="MFGIKRRRTLIRVLSYTISAFALSIIIAVSGFVMAYKFRMTIEY
                     SYQRALSELEDHVSDIDIALQKGMYASTSSQLIGLAGEIWSDAGAAKADMSQLSLSNV
                     QLEQTNKFLAQTGDYANSLSKKLSTNEKITGQERATIKSLSENAGKLSNYLNDIITKL
                     QAGKITLFKSEKVTGKSEKEIQTSVSDGFQHIEASMSNLPSMIYDGPFSDNILKKQPS
                     FTAGKPLVSRSQARKSAADFLKVGVGSVKDSGETGGNLTTYNFSVGTIYIYVSKNGGY
                     VVRMIDSRQIGTAKLSEAAADKKANAFVTSRGYKTLTKSYQLTNNNIMMVNFAYTDGN
                     IICYPDLIKVGISLDNGQVVSFDATGYLMNHKTRKFPAISVSRTYIQGLLNPLLKTKK
                     VSLALIPSGGGGEKLCYEFKCVNDKGQNVIDYFNVSTGVEEQIMILIETAGGSLAM"
     gene            1704..2957
                     /locus_tag="VHR42_06320"
     CDS             1704..2957
                     /locus_tag="VHR42_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3026824.1"
                     /translation="MRERHFHSKNNGVSFDEFFHRTRGKPVSPAGEKQSKNSPFLRKF
                     FCAVILAVVLIFIVGFYGADLMRFAEIHWAVGRRALAGTSSGGSSLVLNKKPTGKDIR
                     MATEEVAKKICPSVVGIIQYKEGKIGESGEGSGIIMNSGGYIITNYHVIEDASRLMVV
                     DWNKKEYSAKVVGSDSRTDLAVVKISGAGFTYAQFGNSDQCQVGEQVVAVGNPSGLKL
                     AGSVTQGIISALNRNVDVGNGPMNLIQTDAAINPGNSGGALVNMYGQVIGINSAKISH
                     EGYEGIGFSIPVNTAKPVIDSILKYGYVKGRVKFGFDCREIDQVTADLNQVPEGIYID
                     YVEKNSGAQKSGVKADDIVTAIDGVKIKSSETLITERDKHKPGDVITLGIYRRKLQKY
                     LTIPVKLMEDRGDAAAQETFETAGW"
     gene            3122..3436
                     /gene="trxA"
                     /locus_tag="VHR42_06325"
     CDS             3122..3436
                     /gene="trxA"
                     /locus_tag="VHR42_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX3026825.1"
                     /translation="MAVITVTSQNFEEEVMQSKLPVLIDFWAPWCGPCKMVSPIVDEI
                     ATEGEGSLIVGKINIDEEPDLATRFNVFSIPTLIVVKDGEVQATSVGARAKEDIKQTL
                     GI"
BASE COUNT         1080 a          801 c         1081 g          917 t
ORIGIN      
        1 gcagagacaa gaaaatttta cggcgccgga gccgtgcatc ggccatatcg gaatatttta
       61 ggccgtcagt tcaaacctgg tggcatattt ttcttttttc ttggaaaaaa tagagaagtc
      121 agaatcgtcg cggaaattgc ggaacgcgcc gttttatgca aataaaagcg ggaggattaa
      181 cgcatgtttg ggattaaacg aaggagaacg ctcatccggg tgctgagtta tacgatatcg
      241 gcgttcgctc tgtcgatcat cattgccgtt tcgggctttg tcatggctta taaatttcga
      301 atgaccattg aatattctta tcagcgcgcg ttaagcgaac tggaagacca tgtgagcgat
      361 attgatatcg cgcttcagaa gggcatgtat gccagcactt catcgcagct tatcgggctc
      421 gcgggtgaaa tctggtcgga tgccggagcg gcaaaggccg atatgtcgca gctttccctt
      481 tccaatgttc agcttgagca aacaaacaaa tttctggccc aaaccggtga ttatgcgaat
      541 tcgctttcca aaaaactttc aaccaatgaa aaaattaccg gtcaggagcg ggcgaccatt
      601 aagagccttt ccgaaaacgc cggcaagctt tccaattatc tgaacgatat tatcacaaaa
      661 ctgcaagccg gaaaaatcac gctgttcaaa tcggaaaagg tgacgggaaa gagcgaaaaa
      721 gaaattcaaa cctcagtgtc cgacggattt cagcatattg aagccagcat gtccaatctg
      781 ccgtccatga tttacgacgg cccgttttcc gataatattc tgaaaaaaca gccgtccttt
      841 accgccggga agccgctggt cagccgcagt caggcaagaa aatccgccgc ggactttttg
      901 aaggtgggcg taggcagcgt aaaagacagc ggagagaccg gaggtaatct gacgacctat
      961 aatttctcgg tgggaacgat ttacatttac gtttccaaaa acggaggcta tgtcgtccgg
     1021 atgatcgaca gcaggcagat cgggacggcg aagctgagtg aggcggccgc cgacaaaaag
     1081 gcgaatgctt ttgtgacgtc cagaggctat aagacgctga cgaaatccta tcaattaacc
     1141 aacaataata ttatgatggt gaactttgct tacaccgatg gaaatatcat ctgttacccg
     1201 gatttgatca aggtcgggat ctcgcttgac aacggccagg tggtatcgtt tgacgcgacc
     1261 gggtacctga tgaatcataa aacgagaaaa ttcccggcca tcagcgtatc gcggacctat
     1321 attcagggtc tgctcaatcc gctgctgaaa acgaaaaaag tgtcgctggc gctgattccg
     1381 tccgggggag gcggagaaaa gctctgttat gaattcaagt gtgtcaacga taagggccag
     1441 aatgtcattg attattttaa tgtgtctacc ggtgtggagg aacaaattat gatcctgatt
     1501 gagaccgccg gaggatcgct cgcaatgtag aactgtaaaa agcagacgct gatcagcgtc
     1561 tgctttttgt tatgccggac gatcatcccc tgaaataaaa tcatttatcc tctgacggcg
     1621 tcataaaatt tgatatggcg tccggataat cggcccatga tatggccgtt tgacggcaca
     1681 ttcagtttta aggggatggg tcgatgcgtg agaggcattt ccacagtaaa aataatggcg
     1741 tcagctttga tgagtttttt caccggacgc gcggaaagcc ggtgagcccg gccggtgaaa
     1801 aacaaagtaa gaattcgccg ttcttacgga agtttttttg cgcggtgatt ctggccgtgg
     1861 ttttaatttt catcgttgga ttctatgggg cagatctgat gcgatttgcc gaaatccact
     1921 gggcggttgg ccggagagcg ttggccggca cctcttccgg ggggagcagt ctggtcctga
     1981 ataaaaagcc cacggggaaa gatatccgaa tggccaccga ggaagtggcc aaaaaaatct
     2041 gccccagtgt tgtgggaatt attcagtaca aagagggtaa gatcggcgag tcgggtgagg
     2101 gctctggaat tattatgaat tccggcggat atatcatcac caattaccat gtgattgaag
     2161 acgccagcag actcatggtt gtcgactgga acaagaagga gtattccgcg aaggttgttg
     2221 ggagcgactc cagaaccgat ttggcggttg tcaagataag cggagccggg tttacttacg
     2281 cgcagttcgg caactcggac cagtgccagg tgggcgagca ggtggttgcg gtcggaaacc
     2341 cgtcggggct gaagctggcc ggatcggtca cgcaggggat tatatcggcg cttaaccgga
     2401 atgttgacgt tgggaacggc ccgatgaatc tgatccagac cgacgccgcc ataaatccgg
     2461 gaaattccgg cggggctctg gtcaatatgt atggccaggt tataggcatc aattcggcaa
     2521 agatctctca cgagggctat gaaggtatcg gcttttccat tccggtcaat accgccaagc
     2581 cggtgattga cagtattctt aaatacggct acgtcaaagg cagggtaaaa ttcggttttg
     2641 actgcaggga aattgatcag gtgacggctg atttaaatca agttcccgag ggcatctata
     2701 ttgactatgt ggaaaaaaac agcggtgcgc aaaaaagcgg cgtcaaggcg gatgacatcg
     2761 ttacggcaat tgacggggtg aaaataaaat catccgaaac attgattacc gagcgggata
     2821 agcataagcc gggggacgta ataacgctcg gaatttaccg gcgtaaactt caaaaatatc
     2881 tgaccatccc ggtgaaactg atggaggaca ggggcgatgc ggcggctcaa gagacgttcg
     2941 aaaccgcagg atggtgatca aagatagaaa aatgtggaac agatgatttt tttatagcgt
     3001 tttttatggt ataatcgggt taaaataatt aatattattt gtttactgta acaaggtcac
     3061 ataaaataat tcaaaaatcc gttatcatca aggcaggaaa gtgaagaaag aggagataaa
     3121 aatggcggta attacggtaa caagtcaaaa ttttgaagaa gaagtcatgc agtccaaatt
     3181 accggtgctg attgactttt gggctccctg gtgcggccca tgcaaaatgg tttcaccgat
     3241 cgtggacgaa attgccacgg aaggagaagg cagcctgatt gtcgggaaaa tcaacatcga
     3301 cgaagagcct gatctggcta cgcgttttaa tgtgttcagc attccgacgc tgattgttgt
     3361 aaaagacggg gaagtccagg caacctctgt cggagcgcgg gcaaaagagg atatcaaaca
     3421 gaccctcggc atctaaaacc gattcaaaca cagctgcgaa aattttcgcg gctgtgtttt
     3481 ttgcgtctga caggattttt atcggagtaa gaacccgatt ggccggaaag acgatgggaa
     3541 gaccgcccgg ccggggtggc ggttttcggc gacacttgtt ataatggatt catacggaag
     3601 tggtatcgga aagtgtttag ctgaaggaat gatattatgg gattgattca gacagtcagg
     3661 aacagaatgg attactggaa agacagggga gaggttcctc cggttaccgc aatcgttgta
     3721 gccgccggaa aaaggagaaa gaatgggggg cgaggtcagc aaacagctgc tgtcggtctg
     3781 tggaatgccg gtgatcgcca gaacgcttgg agcttttgaa aactgccggt cggtgaatga
     3841 ggtcgttgtt gtcgcccggg atgaagatat ggttgagat
//
LOCUS       DATALY010000223         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_653925,
            whole genome shotgun sequence.
ACCESSION   DATALY010000223 DATALY010000000
VERSION     DATALY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653925"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VHR42_06330"
     CDS             complement(<1..235)
                     /locus_tag="VHR42_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026826.1"
                     /translation="MIDKIGVSSTGSLYSSDTRKSESTSKSEKSGFAADAVTIGSSTV
                     SAGIYSKSTNVIYDRATIDKLKQDAEDAKENLRA"
     gene            complement(393..752)
                     /locus_tag="VHR42_06335"
     CDS             complement(393..752)
                     /locus_tag="VHR42_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX3026827.1"
                     /translation="MKKGTTEEVEVIISKSLQVGVLLSAAIMLTGFLLFLITGNSGYP
                     DNTYPTNLAVILQGVLAFKPYAIILAGLLVLILTPVFRVGVSIFVFLKEKDYLYVKIT
                     ALVFVILIISFLLGKVE"
     gene            complement(752..1591)
                     /locus_tag="VHR42_06340"
     CDS             complement(752..1591)
                     /locus_tag="VHR42_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3026828.1"
                     /translation="MVFTIIALKIFLIAILAGFLGSLLGLGGGIIITPVLTLLFGFDI
                     KYAIGASIVSVIATSSGAAITYIKDRITNIRIGMFLEIGTTLGAITGAFLSGIIHPKY
                     LYIIFGILLLYSAVMMLKKAKTELPQNVRNHPLAEKLALNGEYYDKVLGQQVAYQVDG
                     VYGGLGMMYAAGLISGLLGIGSGIFKVMAMDLLMKIPLKVSSATSNFMIGVTAAASAG
                     VYFLRGDIDPKIAAPVALGVLFGAMFGTKAMQRLRSKTIRLIFVPVLAIVSIQMMFKG
                     VQM"
     gene            complement(1593..2759)
                     /locus_tag="VHR42_06345"
     CDS             complement(1593..2759)
                     /locus_tag="VHR42_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3026829.1"
                     /translation="MTDFLTGLGRREIELLNTIRKNGPVTKNGLIEKTGARLTTLNRS
                     MKLLENQKLIAETGTSVSTGGRKPIEYDVTGDSAFAIGVDISRTYTRVIAVNLKMHIL
                     KESRFDMDETITPRECADRIASILKQMTSTLSSDQAKVLGVGIGTVGPMDRNSGVLLH
                     PQGFRNPEWNNEVLLKDLLQKKTGLPCEIDNGANTAVLAEFLSGSGRGYHCVAYIHCG
                     VGIRTAVIQNGTILRAMNDNDDAFAHMTIDQNGPPCLCGGKGCLENYVSLASINARYA
                     AITGNSVNDKELFRRAAGLEKEAADAFQKSAGTLGIGISNLAKLMNPDLIILSGPLIA
                     NYAPYYSICVDTFTEWNNRNSPVIFQKEGFFKENAVAIGAGLMVIERSFNQTRR"
     gene            2915..3079
                     /locus_tag="VHR42_06350"
     CDS             2915..3079
                     /locus_tag="VHR42_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026830.1"
                     /translation="MEEKRKGKQDAQKKAEEAADAFVNGTSFGADPSGSYTGKPKDRG
                     QIPEQDADDL"
     regulatory      3197..3284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            3332..3709
                     /gene="crcB"
                     /locus_tag="VHR42_06355"
     CDS             3332..3709
                     /gene="crcB"
                     /locus_tag="VHR42_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX3026831.1"
                     /translation="MNWNRVLIVGLGGFLGAALRYIISGATTKYWGDFPVGTLIVNVF
                     GGLLMGFIMEASISLWPVSPGVRIFLTTGILGGLTTFSTFSYETISFFSDGEYLMGGL
                     NAGLNLFLALFACWIGKVIAQLL"
     gene            3722..4060
                     /locus_tag="VHR42_06360"
     CDS             3722..4060
                     /locus_tag="VHR42_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX3026832.1"
                     /translation="MTGKGKLLKIYIGESAGHHGEPLYHVIIKKIKANGLAGATTLRG
                     IEGYGVNSVIHSTRILSLSEDLPIVIEVVDTEEKIRKLVGILEDTVAGGILMALQDVE
                     IIWPAGAESK"
     gene            4358..>4603
                     /locus_tag="VHR42_06365"
     CDS             4358..>4603
                     /locus_tag="VHR42_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026833.1"
                     /translation="MKAKRFLSVLASFMILVTAVPASSMAANVPSGSAKASVSVKMYS
                     AKSTVRSVSGVYTVNKMPYRYNATTAAAITIKGNRNQN"
BASE COUNT         1195 a         1050 c         1145 g         1213 t
ORIGIN      
        1 aggcgcgcag attctccttg gcatcttccg cgtcctgttt gagcttgtca atggtggcac
       61 ggtcgtatat tacgttggtt gatttcgaat aaataccagc tgaaacagtc gatgagccga
      121 tcgttaccgc gtcggcggca aaaccgcttt tttcggattt cgatgtagat tcggattttc
      181 gtgtgtcgct tgagtaaagg ctgcctgttg agcttacgcc gattttatcg atcaaaaaaa
      241 tccctcctca acaactatat cggaggatgc cgattgaaac ttgagagaag taacgaaatc
      301 tgcgttaaag ttgtgaaaac aagaccgatg gatttcattt tccagcggtc ttgtcgagtg
      361 gcgtctggtg gcgactgtct ttgtaattcg ggttattcca ctttgccgag aaggaaactg
      421 atgattaaaa tcacaaacac cagcgcggtg atttttacgt ataaataatc cttctccttc
      481 aggaagacaa aaatagagac gccgacgcgg aaaaccggag ttaaaattaa tacaagcaat
      541 ccggcaagaa taatcgcgta aggcttgaat gcgaggactc cctggaggat gactgcaaga
      601 tttgtgggat aagtattatc ggggtaaccg ctgtttcccg tgattaaaaa tagaagaaag
      661 ccggtcagca taatggcggc gctcaataaa acgccgacct gcaaagattt gctgatgatc
      721 acttccacct cttccgtcgt gcctttcttc attacatttg cacccccttg aacatcatct
      781 gaatcgacac aatcgcgaga acagggacaa aaatcagtcg gatcgtctta ctgcgaagcc
      841 gttgcattgc ctttgttccg aacattgcgc cgaacagcac gccaagcgct accggggcgg
      901 caattttggg atcaatatca cccctcagga agtaaacccc tgcgcttgcg gcggcggtca
      961 cgccgatcat gaaattgctg gtggcgctcg aaaccttgag cggaatcttc atcaataaat
     1021 ccatggccat gactttgaaa atgccgctgc cgatcccaag cagacctgag atcagaccgg
     1081 ccgcatacat catgccgagg ccgccgtaga cgccgtccac ctgataagcg acctgctgcc
     1141 cgagaacttt atcgtaatat tcgccgttca gggcgagctt ttccgccagt ggatgattcc
     1201 ggacattttg gggcagttcc gttttggctt tcttcagcat catcacggcg gaataaagca
     1261 gaaggattcc gaaaatgatg tacaagtatt tcgggtggat aataccgctc aaaaaggccc
     1321 cggtaatcgc accgagcgtg gttccgattt caaggaacat cccgatacgg atatttgtga
     1381 ttcggtcttt aatataggtg atggccgcgc cgctggaagt ggcgataacc gatacaatgc
     1441 ttgccccgat tgcgtatttg atgtcaaagc cgaaaagcag cgtcaaaacc ggtgtaatga
     1501 tgattccgcc tcccagcccc aaaagcgacc ccagaaatcc cgccagaatg gcgattaaaa
     1561 atattttcag cgcgatgatc gtaaacacca ttttatctcc ttgtctgatt aaaactgcgc
     1621 tcgatgacca taagtccggc gccgatggct acggcatttt ctttgaaaaa gccttctttt
     1681 tgaaaaatca caggactgtt tcgattgttc cattcagtaa aagtgtcaac acaaatcgaa
     1741 taatagggag cgtaatttgc aataagcggc ccgcttagaa tgatgaggtc gggattcatt
     1801 agttttgcca gattcgagat gccgatgccc agagtcccgg cgcttttttg gaacgcgtca
     1861 gcggcttctt tctcaaggcc ggccgcgcgg cggaaaagct ccttatcatt cacagaattc
     1921 cccgtaattg cggcgtaacg tgcgttgatg gaggcaagag acacatagtt ttccagacag
     1981 cctttgccgc cgcacaggca gggcgggccg ttttgatcga tcgtcatatg ggcgaaagcg
     2041 tcgtcattgt cattcatggc cctgagaatc gtaccgtttt gaatgacggc cgtccgaatc
     2101 ccgacaccgc agtgaatata agcgacgcag tgatatccgc ggcctgagcc ggatagaaat
     2161 tccgccagca cggcggtgtt tgcgccgtta tcgatctcac aggggaggcc ggtctttttt
     2221 tgcagaagat cttttaataa gacctcgtta ttccactcgg gattacgaaa cccctgggga
     2281 tgcagcagta cgccgctgtt tctgtccatc gggccgacgg ttccgattcc gacgcctaaa
     2341 accttcgcct ggtccgacga aagggtcgaa gtcatctgtt ttaggatgct cgcaattctg
     2401 tcggcacatt cccggggcgt tatggtctca tccatatcaa aacgggattc ttttaaaatg
     2461 tgcattttta aattgacggc gatgacccga gtgtaagttc ttgaaatatc cacaccgatg
     2521 gcaaatgcgc tgtcgccggt aacgtcatat tcaatcggtt tcctgccgcc ggtagaaaca
     2581 gaagtcccgg tttccgcgat cagtttctgg ttttccaaca gcttcattga ccgattgagc
     2641 gtcgtgagtc ttgcccctgt tttttcaatc agaccgtttt tggtaacagg cccgtttttt
     2701 cgaatcgtat tcagcagctc aatttccctg cggccgagcc ctgtcaaaaa atcagtcatg
     2761 acatcccccc ctgtgtgatc gtttaccgat tctattataa acacttttaa ccaaaatggc
     2821 aataagtaat caacccggat ttttggacgg gcaccggttt gatttactga atttttactt
     2881 ttcgtccggt cacaataaac acgaggtgaa tcagatggaa gaaaaacgaa aaggaaagca
     2941 ggacgcgcaa aagaaggcag aggaagcggc tgatgctttt gtcaatggaa cctcttttgg
     3001 cgcggatcca tccggcagtt atacgggtaa accaaaagat cggggccaga ttccggagca
     3061 ggacgcggac gatctctgag ccttatgtct ttagaatgaa gtcgaaagtc ggagcggaat
     3121 aagcacgaaa ttaagtatat cggttaataa taatttgaaa attgtcttta caaaaccacc
     3181 cgaatcatat attctaatct atattggtga tgaggctcac cgggagctta tgcagacaat
     3241 gataagatcc caatgcagag gatgctaatg gcttctactt tgagcgatta aagtagaagc
     3301 cttatctttt tgccttgaag gtggtataat tatgaattgg aacagagtgc tgattgtcgg
     3361 tcttggcggc tttttaggcg ccgcgctgcg ttatatcatt tccggagcca caaccaaata
     3421 ttggggcgat tttccggttg gaacactgat tgtaaatgtt ttcggcgggc ttctgatggg
     3481 tttcattatg gaggcgagta tcagcctgtg gccggtttcc ccaggtgttc gtatttttct
     3541 gactactgga atcttgggcg gtctcacgac cttttcgacc tttagctatg aaacgatctc
     3601 attcttctca gacggagaat atctgatggg agggttgaat gccgggctca atctttttct
     3661 tgccctgttc gcatgctgga tcgggaaagt aattgcgcag ttgctgtaag gaggcgtctg
     3721 tatgacagga aaaggcaagc ttttgaagat ttacatcggc gagtctgcgg gacatcacgg
     3781 cgagcctctg tatcatgtta tcatcaaaaa aatcaaagcg aacggccttg cgggtgccac
     3841 gacccttcgg ggaattgagg gatatggagt caacagcgtg attcacagta cacggattct
     3901 gagcctttcc gaagatcttc caatcgtgat tgaggtggtg gatacggaag aaaaaattcg
     3961 gaagcttgtt ggaattctgg aggatacggt tgccggtgga attctgatgg ccctccagga
     4021 cgtggaaatc atttggccgg cgggagccga atcaaaataa tgcggagtaa tgcgacagcc
     4081 ggtccctggt ttccaggttc gttggctgtc gtttttttta tgccattttt aaaatattat
     4141 cggtaagtta agccggaaag gaccgacgct ttttatgcct gattcacccg ttttgtaaac
     4201 aaaaattaaa cgaataaaag caaagcggtt tgccggtaac aaacacatgg aaaatattaa
     4261 ctgaactgtt atttagctga taagcatttt gtataccggt atgctatcat tcttattagg
     4321 ggctaacccc tgaaataatt gaatgacagg tgagaacatg aaagcaaaaa ggtttttgag
     4381 cgttctggcg tcctttatga ttctggtaac ggcagtgccg gcgtcctcaa tggcggcgaa
     4441 tgtaccgtca ggatcggcta aagcttcggt tagcgtcaaa atgtattccg caaaatccac
     4501 agttcggtcc gtgagcggcg tttatacggt caataaaatg ccttatcgtt ataacgcgac
     4561 gaccgctgcc gctattacca taaagggaaa cagaaatcag aat
//
LOCUS       DATALY010000224         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_565537,
            whole genome shotgun sequence.
ACCESSION   DATALY010000224 DATALY010000000
VERSION     DATALY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565537"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VHR42_06370"
     CDS             <1..844
                     /locus_tag="VHR42_06370"
                     /inference="COORDINATES: protein motif:HMM:NF040016.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HEX3026834.1"
                     /translation="LETEFCKCFSLRSIIDFLRGYSVKFQFDVTQSPLNLFEILFDQA
                     VFTVLSENSVGLSVSLPGFQSMRNRLSALSSQEIRLEIRRAVEKIVSEFSLGNPRIVK
                     YLRRSRLRLTRRFLSAFNGDNLQLMAVIDGENPNEGKILFEDGERMNDEDFSNLVELA
                     SQCSETPEKIQLARGIHSFWDFIDFLESDCLYGEEYIQIFESLGNEELAVLGSFLFEE
                     ELRIGPFQLTDRVIDQYHHLTQQDWQTHYLNYIRSLDPTKKSLMEDLINRMILPSNNA
                     RDFQ"
     gene            1237..2655
                     /gene="hydG"
                     /locus_tag="VHR42_06375"
     CDS             1237..2655
                     /gene="hydG"
                     /locus_tag="VHR42_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HEX3026835.1"
                     /translation="MYNPKSKTATEFIDDQEIMETLEYAKHNSRNRELISSLIERAKD
                     CKGLTHREAAVLLECDLEDENEKIFKLAKQIKQNIYGNRIVLFAPLYLSNYCVNGCVY
                     CPYHHKNKNIGRKKLSQEEIVREVVALQDMGHKRLALETGEDPINCPIEYVLESIHTI
                     YGIKHKNGAIRRVNVNIAATTVENYRKLKDAGIGTYVLFQETYHKKSYEELHPTGPKH
                     NYAYHTEAMDRAMEGGIDDVGIGVLFGLNLYRYDFVGMLMHAEHLEAAMGIGPHTISV
                     PRIRPADDIDPKNFSNAISDDIFAKIVAVIRIAVPYTGMIMSTRESQKTRERVLDLGI
                     SQISGASKTSVGGYDEPEDEDENTAQFEVNDNRTLDEVINWLLKLDYIPSFCTACYRE
                     GRTGDRFMRLAKTGQIQNCCHPNALLTLKEYLMDYASDATRKIGKEKIEKEINHITNE
                     KVRTVAVHHLAEIQNGKRDFRF"
     gene            complement(2752..>5248)
                     /locus_tag="VHR42_06380"
     CDS             complement(2752..>5248)
                     /locus_tag="VHR42_06380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026836.1"
                     /translation="GSTDGSAELISSKYTWIKLIKNQKNEGFAKPSNDGARRATGEYV
                     AFLNNDMKVERDWLIELVKSMESNNASCAGSVIMNWNGELLDFAGGSISFYGMGYQYH
                     FHESMDKVESLLTQDKELLFSCGGAMIVKRDLFLRIGGFDEDYFAFFEDIDLGWRLNV
                     LGEKVVLSVKSRAYHKHHSTGNRFSKERMQVLYERNSLYTIYKNYGEELLNKAFWPAI
                     LMNNANAFGRSGIDRDEFDLRISSKKFDDKGTFISNLAAAQLCALHDFVKNLNKMTEK
                     RRYIQANRKISDEKIIRYINDPFICLGKDLNQFNLIKYDFAKNFGIDKAFNMDLKRRV
                     LLVSSDTIGKKMAGPAIRYYEFAKSLSKSCDVVLASCGTCDIESDQFKIIDYSYEHSE
                     NLTFEAMQSDIIVTQGFTLEFCKQFEYISKSRYLIIDLYDPYVIENIEVFKDHEIKRR
                     REDFEFSSSAIINQLKSGDFFVAANSKQKDYWLGMLSSLKRVSPEVYDISHNYNRLID
                     VVPFGISDTAPAHKKNVLKGVWPGISKDDHVLIWGGGVWNWFDPITLIKGMAVIAQKR
                     SDIKLFFLGVKHPNPNIPEMKMLNDAVELAKETGLYNKSVFFNFDWVDYDDRQNYLLE
                     ADIGVSCHFDTIETRFSFRTRILDYLWAGLPIISTKGDYFSDLVEKEKLGITVDFKDE
                     QQIADAVIRLIDDREFYEQCKKQISRVAEGYKWSKITKPIVDFCSDPIHLSGTTKDQT
                     EEEDSENENTEDKSSKSPMRKGSIYQMLYSIAQRQGAIEKQLRRNTMINEKIYHKTSE
                     IQEWSYMMNGRFNKIKKYANPFKLIARLFRRRK"
BASE COUNT         1450 a         1254 c         1076 g         1468 t
ORIGIN      
        1 gctcgaaacc gagttttgca aatgtttttc tctcaggagc atcattgact ttctcagggg
       61 ttattcggtt aaatttcaat ttgacgtcac acagtctccc ttgaatcttt ttgaaatcct
      121 gtttgatcag gcggttttta cggtactatc agagaactcc gtcgggcttt cggtatctct
      181 gccgggcttt cagtcgatgc gaaatcgcct ttcagcgctt tcctcccagg aaatccgtct
      241 tgagatccgc cgtgctgttg agaaaatcgt ttcagaattc agccttggaa acccccggat
      301 tgtgaaatat ctgcgtcgtt cccgcctccg gctgacccgg cgcttcctga gcgctttcaa
      361 cggcgataat ctccagctta tggctgtaat tgacggtgaa aacccgaatg agggcaagat
      421 cctgttcgaa gacggagaac gcatgaacga cgaggatttt tcaaatttgg tggagcttgc
      481 ctcccaatgc agcgaaacgc ctgaaaaaat ccaacttgca cgcgggatcc attcgttttg
      541 ggattttatt gattttcttg aatccgactg cctgtatggc gaggaatata ttcagatttt
      601 tgaatcgctg ggtaacgagg agcttgccgt tttgggaagc tttctgtttg aagaagagct
      661 gcgaatcggc ccgtttcaat tgactgatcg tgtgattgat caatatcacc atctgacaca
      721 gcaggattgg cagacgcatt atctcaatta catccgctct cttgatccaa cgaaaaaaag
      781 tcttatggaa gatttaatca atcggatgat tctcccgtct aataacgccc gggatttcca
      841 ataacgacag atcatcgtta tcgtctattt gttaatcttt taacgcaata ggcttgatat
      901 tcaagtcagg tgtgttataa taaatctaag atcagccggt gatccgacga gttggggcca
      961 gcacccttct tacggagaac cgtatgagcg gccacgaaaa agtatctttc agaccacaag
     1021 ggttcgtccc gagtgggaag gcgagagtag agctgaatga gtaaggaaag ctcggccttt
     1081 ttttacggtg cttccctttt tatctgctat ccctgcgcct ttcggcgcag ggatttttat
     1141 tgggaaagaa gaaataaatt gcccatcaaa aataatcaca ggcttttccc gggaaataaa
     1201 agtaaaccag cagatagatc aaaaggagtg tttatcatgt ataatccgaa atcaaaaaca
     1261 gcaaccgaat tcattgacga tcaggaaatt atggaaactc tggaatacgc gaaacacaac
     1321 agccgcaacc gggaactgat cagcagcctg attgagcgtg caaaagactg caagggactg
     1381 acccaccggg aagccgcggt tcttctggaa tgcgatctgg aagacgaaaa cgaaaaaata
     1441 tttaaacttg caaagcagat taagcaaaac atttatggta atcgaatcgt tctgttcgct
     1501 ccgctttatc tctccaacta ctgcgtcaac ggttgtgtct actgccccta ccatcataaa
     1561 aacaagaata ttggacgcaa aaagctttcg caggaagaga tcgtccgcga agttgtcgcc
     1621 ctgcaggata tggggcataa acggctggct cttgagaccg gtgaagatcc gatcaattgt
     1681 cccatcgaat atgtattgga gagcattcat acgatttatg gaatcaaaca taaaaacggg
     1741 gcaatccgta gagtcaacgt caacattgca gccaccaccg tggaaaacta ccgcaagcta
     1801 aaggacgcag gtatcggcac ctatgtactg tttcaggaaa cctatcataa aaaaagttat
     1861 gaggagcttc atccgaccgg cccgaaacat aattacgctt atcacaccga agcgatggac
     1921 cgcgccatgg aaggcggaat cgacgacgtg ggaatcggcg ttttattcgg tctgaatctt
     1981 taccgttatg attttgtcgg gatgcttatg catgccgaac atctggaagc cgcaatgggc
     2041 atcggacctc acaccatcag tgttccccgc atccgtccgg cggacgacat cgatccgaaa
     2101 aatttttcaa acgctatatc cgacgatatt tttgctaaga ttgtagccgt tattcgtatt
     2161 gccgttccct acaccggcat gattatgtca acccgtgaat ctcaaaaaac ccgtgaacgt
     2221 gttctcgacc tgggtatttc acaaatcagc ggcgcctcca aaaccagcgt tggcggatat
     2281 gatgagccgg aagatgagga tgaaaacacc gcccagtttg aagtcaacga caaccgtact
     2341 cttgatgaag tgatcaactg gctgcttaaa ctggattata ttccaagctt ctgcaccgcc
     2401 tgttaccgtg aagggcggac cggtgaccgt tttatgcggc tcgccaaaac agggcagatt
     2461 caaaactgct gccatcccaa cgcgttgctg accctgaaag agtatctgat ggattacgcg
     2521 tctgacgcga cccgcaaaat aggtaaagaa aaaatcgaaa aagaaatcaa tcacatcacg
     2581 aatgaaaagg tacgcacggt cgcagtccat catttggccg aaatccaaaa cggcaaacgc
     2641 gatttcaggt tttaacctgt tttatactta ttggtaatac agcaccgaaa acggcccggc
     2701 ttccactttc ttggaagccg ggccgctttt ataaaatttt tggatacaat gctactttct
     2761 tcgcctgaac agccgcgcaa tcagcttaaa cggattcgca tattttttga tcttgttaaa
     2821 ccggccgttc atcatatagg accattcctg gatttcggaa gtcttatggt atatcttctc
     2881 attgatcatg gtatttctcc gaagctgttt ttcaatagcc ccctgccgct gcgcgatact
     2941 atacagcatc tggtatattg agccctttct catcggagac tttgatgatt tgtcttccgt
     3001 gttttcattt tccgaatctt cctcttccgt ctgatccttc gttgttccgc tcaagtgaat
     3061 gggatcgctg caaaaatcga cgattggttt tgtgatcttg ctccatttgt aaccttcggc
     3121 tacccgtgaa atttgtttct tacactgctc atagaattcc cggtcgtcaa tcagcctgat
     3181 tacagcgtcc gcgatctgct gctcatcctt gaagtccacc gtaataccaa gcttttcttt
     3241 ttcaaccaga tccgaaaagt aatccccttt ggttgagata atcggaagac ctgcccataa
     3301 atagtccagg atcctggttc ggaacgaaaa tctggtttca atcgtgtcaa aatggcagct
     3361 cacgccaata tccgcctcaa gcagataatt ttgtctgtcg tcatagtcga cccaatcgaa
     3421 gttgaaaaaa acagacttat tataaagacc tgtttctttt gcaagctcaa ccgcatcgtt
     3481 aagcatcttc atttccggga tattaggatt aggatgtttg acgccaagaa aaaacaattt
     3541 gatatcggac cgtttttgcg caatgacagc catgcccttg atcagcgtaa tcgggtcaaa
     3601 ccagttccag acgccgcccc cccagatcag gacgtgatcg tccttgctaa tgcccggcca
     3661 aactccctta agtacgtttt tcttatgggc aggcgcagta tccgaaatgc caaacggcac
     3721 aacatcaatc agtctgttat aattgtgact gatgtcatac acttcgggcg aaacccgttt
     3781 cagggatgaa agcatgccaa gccaatagtc cttttgtttg ctgtttgccg caacaaaaaa
     3841 gtctccgctt ttcagctggt ttataatcgc agaagacgaa aactcaaaat cctcccgcct
     3901 tcttttaatt tcatgatctt taaaaacttc tatattttca atgacatatg ggtcatacag
     3961 atcaataatc aaataccttg attttgaaat atactcaaac tgtttgcaga attctaatgt
     4021 aaacccctgg gttacgataa tgtcggattg cattgcttca aaagtcagat tttcagaatg
     4081 ctcgtaagaa taatcaataa ttttaaattg atccgattca atgtcgcatg ttccacatga
     4141 cgctaataca acatcacaag atttgctcaa cgatttggca aattcataat atcgaatcgc
     4201 cggccccgcc atttttttcc cgatcgtatc actcgaaacc agaagaaccc gacgcttaag
     4261 atccatgtta aacgctttgt ctataccgaa atttttcgca aaatcatatt taatcagatt
     4321 gaattggtta agatcctttc ccagacaaat aaacggatca ttgatataac gaatgatttt
     4381 ctcatcgctg attttccgat tggcctggat gtatcgccgc ttttcggtca ttttatttag
     4441 atttttcaca aaatcatgga gtgcacataa ctgcgctgct gcgagatttg aaataaaagt
     4501 acctttatca tcaaactttt tacttgatat tctaagatca aactcatccc ggtcaattcc
     4561 tgaccgtcca aaagcattgg cattattcat cagaattgcc ggccagaaag ctttgttcag
     4621 aagctcctcg ccataatttt tgtagatcgt gtaaaggctg ttcctctcat aaagcacctg
     4681 catgcgctct ttggaaaacc ggtttcctgt gctatgatgt ttatggtatg ccctggattt
     4741 tacagagagt actacctttt cacccaacac atttaaccgc catccaaggt cgatatcctc
     4801 aaaaaaggca aaataatctt catcaaagcc gccgatccga agaaaaagat cccgtttcac
     4861 gatcattgca ccgccgcatg aaaacaagag ttccttatcc tgtgtcagca acgattctac
     4921 cttatccatc gattcgtgga aatggtactg gtatcccatt ccataaaagc tgatgctgcc
     4981 gcccgcaaaa tcgagcaatt ccccattcca attcataatg accgaacctg cacagctagc
     5041 attgttgctt tccatggatt taacaagctc gatcagccag tctctctcca ccttcatatc
     5101 gttatttaaa aatgcgacat actccccggt agcccttctt gctccgtcat tgcttggctt
     5161 ggcgaatccc tcgttttttt gattcttgat taatttgatc catgtatatt ttgaagaaat
     5221 caactcggcc gatccatcgg tcgatcca
//
LOCUS       DATALY010000225         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_135555,
            whole genome shotgun sequence.
ACCESSION   DATALY010000225 DATALY010000000
VERSION     DATALY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135555"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VHR42_06385"
     CDS             <1..1315
                     /locus_tag="VHR42_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3026837.1"
                     /translation="DMIRCFTEAHKRGMYILLDLVPGHTSDQHPWFLESKKAERNQYS
                     SRYIWTDTVWNRPAGYNSMCGVTERDGCYVLNFFSSQPALNYGFNKITAPWQQHYTNP
                     DCIATREAMKDVMRFWLDAGCDGFRVDMAACLVKNDDDNVATAEIWRNVREMLDRDYP
                     EAAMVSEWSNPDKAINKSRFHMDFYLNHMGNGYHSLFRKTSSDGNPLSFFSKRGQGNI
                     MDFLDDYLPKYNVSKSTGYISFIICNHDTPRMTLGYDESECKIAYSFLLTMPGVPFIY
                     YGDEIGMQYFNVVSVEGGYNRTGTRTPMQWTNGKNLGFSNADKEKLYLPVDGRDCAPT
                     VENQAKDEHSLLNTVRKVLAIRHQIPDLQAVSNLEVIYAEKDCYPFVYRRGKYFIAVN
                     PSCHEVEIPLMEKGESVFTIGGSSLEKGHLMMCGQSFAIFKSTRN"
BASE COUNT          440 a          317 c          381 g          384 t
ORIGIN      
        1 cgatatgata cggtgcttta cagaggcaca taaacgcggt atgtatatct tgctcgattt
       61 ggttcccgga catacgtccg accagcaccc gtggtttctg gaaagtaaaa aggcagagcg
      121 caatcaatac agcagccggt atatttggac ggataccgta tggaaccgcc cagccgggta
      181 caactcgatg tgcggcgtaa cggaaagaga cggctgctac gttctgaatt ttttctcgtc
      241 acagccagcc ctgaattacg gctttaacaa gataaccgca ccgtggcagc agcattatac
      301 aaatccggac tgcatcgcga cacgagaagc aatgaaagac gtgatgcgtt tttggctcga
      361 tgcaggctgc gatggttttc gcgttgatat ggctgcatgt ttggttaaaa atgacgacga
      421 taacgtcgca accgcggaaa tctggagaaa tgtgcgcgaa atgcttgacc gggattatcc
      481 ggaagccgca atggtatccg aatggtccaa tcctgacaag gcaattaaca agtcgcggtt
      541 tcacatggat ttttatctga atcacatggg aaatggttac cattccctct ttcgaaaaac
      601 gagcagtgac ggaaaccctc tcagcttttt cagcaagcgg ggccaaggga atattatgga
      661 tttcctggat gattatcttc cgaaatacaa tgtgtcaaag tcaacaggct atatcagctt
      721 tataatctgt aaccatgata cgccccgcat gacacttggc tatgatgagt cggaatgcaa
      781 aatcgcgtat tcgtttcttt taaccatgcc cggcgttccg tttatttatt acggcgatga
      841 aataggcatg cagtatttta acgtagtaag cgtggaaggc ggttataacc gaaccggaac
      901 gcgtacgcca atgcagtgga caaatggaaa aaatttaggt ttctccaatg cggataaaga
      961 aaagctatat ctgccggtag atggccgtga ctgcgctccg actgtggaaa atcaggcaaa
     1021 agatgagcat tctttgctga atacggtgcg aaaagtgttg gccattcgcc atcaaattcc
     1081 agatttacag gcagtttcaa acttagaggt gatttatgcg gaaaaggact gttatccgtt
     1141 tgtgtaccgc cgtggtaagt actttatagc agtgaatcct tcctgccatg aagtggagat
     1201 tcccttgatg gagaagggag aatctgtgtt cacgataggc ggcagttctt tggaaaaggg
     1261 tcatcttatg atgtgtggac aatcttttgc aatttttaaa agcacccgga actgatatgt
     1321 ggttcgtacc aatagaaaga agtttgcaat tcaactagga tgagtgttcc aaagaggtgt
     1381 ccctttagaa atccacagaa taaaacggga gggaaactgg acagcaggct gaaccgaaag
     1441 tctttcttca gtacgaaagg caaacaggat gcccgaataa taaaaccgta tctgttgctt
     1501 tacaaacggc acagatacgg ca
//
LOCUS       DATALY010000226         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_135571,
            whole genome shotgun sequence.
ACCESSION   DATALY010000226 DATALY010000000
VERSION     DATALY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135571"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VHR42_06390"
     CDS             <1..448
                     /locus_tag="VHR42_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX3026838.1"
                     /translation="LALAAELDMPVIIHDREAHGDAMAILRKYRVKGVMHCFSGSAEM
                     AKELLSMGFYLGFTGSVTFKNNKKAAQVVPIVPAERILIETDCPYMAPEPLRGRRCDS
                     SMLIHTLSHIARLKGLEPEDLAGVTAANARRFFGIHQTSAKAANAR"
     gene            531..908
                     /gene="crcB"
                     /locus_tag="VHR42_06395"
     CDS             531..908
                     /gene="crcB"
                     /locus_tag="VHR42_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX3026839.1"
                     /translation="MQKILYVGIGGFIGAATRYLISIHSAKLFGTQFPVGTLVVNVLG
                     GLLIGLIMELSLATDLIPPNARIFLTTGVLGGLTTFSTFSYETVSLFSEGSYLLGGAN
                     LILNLFLSLCGVVLGKTIVRIMI"
     gene            924..1265
                     /locus_tag="VHR42_06400"
     CDS             924..1265
                     /locus_tag="VHR42_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX3026840.1"
                     /translation="MKIEGKGKLLKIYIGESDRWHHESLYHAIIKVIKQQGLAGATTV
                     RGIEGFGANSRVHNSGIEVLSQDLPIVIEVIDKEDKINEIVGIIEPMITEGLMVLQHD
                     VEIIKYAKSKP"
     gene            1317..>1856
                     /locus_tag="VHR42_06405"
     CDS             1317..>1856
                     /locus_tag="VHR42_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HEX3026841.1"
                     /translation="MTITYRIGKSLYVNITNRCPNRCAFCIRNYDNGVGSADSLWLEH
                     EPSADEILRDILKNDLLAFDELVFCGYGEPLERLDDVVFLCKKIREQSDIRIRINTNG
                     QADLIHQKETAPALKGLVDIISISLNAPDAESYQRLCRSDFGENAFDAVLKFTADCKK
                     HIPRVIVSVVDVIPPDEVEK"
BASE COUNT          456 a          513 c          486 g          401 t
ORIGIN      
        1 gcttgccctc gccgccgaac tggatatgcc ggtgatcatc cacgaccggg aagcccacgg
       61 cgacgccatg gccattcttc ggaaataccg ggtcaaaggc gtgatgcact gcttttcggg
      121 cagcgcggaa atggccaaag aacttctctc catgggtttt tatctcggat tcaccggctc
      181 ggtgaccttt aaaaataata aaaaagccgc tcaggttgtt ccgatcgttc ccgccgagcg
      241 aattctaatc gaaaccgact gcccctatat ggctcccgag cctctgaggg gccgccgctg
      301 tgattcctcc atgctgatcc acacgctgtc ccatatcgcg cgcctcaagg ggctggaacc
      361 ggaggatctg gccggggtca ccgcggcaaa tgccagaagg tttttcggca ttcatcagac
      421 ttcagccaaa gctgcaaacg cccgataatt gggcccgcat tcatcccaac tttcaataaa
      481 ctcctgcttt ccgtccaagg gcgggagttt tctattgccg gaggaaccct atgcagaaaa
      541 ttttatacgt cggtatcggc ggctttatcg gagccgcgac gcggtacctg atctcaattc
      601 attccgccaa actgttcgga acccagtttc ccgtcgggac attggtcgtc aacgtgctgg
      661 gaggattact gatcggcctg atcatggagc tgagcctcgc caccgatctg atcccgccca
      721 atgcgagaat attcctcacc accggcgttt taggcggtct gacgactttc tccaccttca
      781 gctacgaaac ggtcagcctg ttttccgaag gaagctatct gctgggcggg gccaacctga
      841 tcctgaacct ttttctgagc ctgtgcggcg ttgtccttgg caaaacgata gtccgaatca
      901 tgatataaag ggtgatgttt ttcatgaaga tcgaaggcaa gggcaaatta ctgaaaatct
      961 atatcgggga atccgacagg tggcatcatg aatccttata tcacgcgatt attaaagtga
     1021 tcaagcaaca gggcctggcg ggcgccacca cggtcagggg gatcgagggc ttcggggcga
     1081 acagcagagt ccataacagc ggcatcgaag ttttgtcgca ggacctgccg attgtcattg
     1141 aagtaatcga caaggaagat aaaatcaatg aaattgtcgg gatcatagag ccgatgatca
     1201 cagaaggcct gatggtgctc cagcacgatg tggaaattat caaatacgcg aaatccaagc
     1261 cgtgacgcgc cggcagctct gccttaaatc aaaaaagcgg aaggagtaac ctaccgatga
     1321 ccatcaccta tcggatcgga aaaagccttt atgtcaacat caccaaccgc tgcccgaacc
     1381 ggtgcgcctt ctgcatccgg aattacgaca acggcgtggg cagcgcggat tcgctctggc
     1441 tggagcatga gccgtccgcc gacgaaatac tccgcgacat cctcaaaaat gaccttctgg
     1501 cttttgacga gctggttttc tgcggctacg gcgaacccct ggagcgcctg gacgacgtcg
     1561 ttttcctctg caaaaaaata agagagcaaa gcgatatccg gatccggatc aacaccaacg
     1621 gtcaggccga cctgatccac caaaaagaaa ccgcccctgc gctcaagggg cttgtggata
     1681 ttatttcgat cagtctcaac gcgccggacg ctgaaagcta tcaacggctt tgccggagcg
     1741 atttcgggga aaacgctttc gacgcggtcc tgaaattcac ggcagactgc aaaaaacata
     1801 tcccgcgcgt gatcgtttcg gtggtcgacg tgatcccccc ggacgaggtt gaaaaa
//
LOCUS       DATALY010000227         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_224676,
            whole genome shotgun sequence.
ACCESSION   DATALY010000227 DATALY010000000
VERSION     DATALY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224676"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="cheB"
                     /locus_tag="VHR42_06410"
     CDS             complement(<1..875)
                     /gene="cheB"
                     /locus_tag="VHR42_06410"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEX3026842.1"
                     /translation="MSIKKKIRVLVVDDSLLFREAIVRGLSNDAGIEVVGSAQDAFSA
                     RDKILELEPDVMTLDVEMPKMSGVEFLKKLMPQYPLPVVMVTSLSSSLFDALNAGAVD
                     FVSKPDIDSKDDIPSFINELIIKVKIASTAKVGHHKKELRETTNLSGRSFSDRNTIVA
                     IGASTGGTEATLEVIKNLPREMPGIVIVQHMPPVFTKMYADRLNNICKIEVKEARDGD
                     EVLPGRALIAPGSFQMQLIKKQGRYFVKCFEGEKVNGHCPSVDVLFDSVASVAKENAI
                     GIILTGMGNDGAKGL"
     gene            complement(890..1705)
                     /locus_tag="VHR42_06415"
     CDS             complement(890..1705)
                     /locus_tag="VHR42_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX3026843.1"
                     /translation="MLVLKDDEFMQLAQFMKSNYGINLFRKKTLIEGRLSNLVIEKGF
                     SSFGEYIAYAMGDKTGKEVSNLINKLTTNHTFFMREKQHFDYMKEVVLPYLSKNIRDH
                     DMRIWSAGCSSGEEPYTLEMILHDYFGDEKHLWDTEILATDISSRALEKAKTGIYPAE
                     AVNDIPPFWKLNYFQKQNDGNVAVSEKIKKEIVFRVFNLMQNEFPFRKKFHVIFCRNV
                     MIYFDQKTKNELVQKFYDHTEPGGYLFIGHSESIDRELTKYQYIMPAVYRKVI"
     gene            complement(1980..>3296)
                     /locus_tag="VHR42_06420"
     CDS             complement(1980..>3296)
                     /locus_tag="VHR42_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026844.1"
                     /translation="SGGYSDIIHSVNNLLAAVEKPVSQVTEVMTSIAQANFDVEIEGE
                     YNGQFKILIEAVNTTARSLRQTLDRVSEIMIEMSMGNFDFERIEDFDGDFRAVSAALN
                     TILDSLNELLGSINTAAEQVASGSTQVSSGSQQLSEGATTQASSIEELNASISQIAVQ
                     TRTNAENANQANKLAISARENAVKGNREMDQMLQSMVDISQSSKNIFKIIKVIDDIAF
                     HTNILALNAAVEAARAGQAGKGFAVVAEEVRNLASKSAEAAKNTAEMIEGSIRQVDNG
                     TKIADSTSEELKNIVEVIGHAAQLVGDIASASNEQATGISQVDKGIQQVSQVVQTNSS
                     TAEESAAASVELNSQAEQLKELVGKFRLRGITARPFHQTDEDERDLTEVTESFDQPDR
                     LIEPEESHEELLEQTEAAAQKEDEQTEPVSAEPPKTEDDEEEFGKY"
BASE COUNT          786 a          878 c          677 g          955 t
ORIGIN      
        1 aacaagcctt ttgccccatc gttgcccatc cccgtcagaa taatgccgat ggcattctct
       61 tttgcgacag acgcaaccga gtcaaacagc acatctacag acgggcaatg gccgtttact
      121 ttctcaccct caaaacattt cacaaagtat ctgccctgct ttttgatcag ctgcatttga
      181 aagcttcccg gagcgatcaa agcccttccc ggaagaacct catcgccgtc cctggcctct
      241 tttacctcga ttttgcagat attattgagc ctgtcggcat acattttagt gaaaaccggc
      301 ggcatatgct ggacaataac aatacctggc atctcccttg gcaggttttt aatcacctcc
      361 agagtagcct cggttcctcc cgttgacgcg ccgattgcaa cgatcgtatt cctatcggaa
      421 aatgatcttc ctgacaaatt tgtcgtctca cgaagttcct ttttatgatg gccgaccttg
      481 gctgttgaag caattttcac cttaatgatc agttcattga taaaagaagg tatatcgtcc
      541 ttggaatcta tatccggttt gcttacaaaa tctacggctc cggcattcag cgcgtcgaac
      601 aagctgctgc tgagactggt aaccatcacg acaggcaacg gatactgcgg catcagcttt
      661 ttaagaaatt caacgccgct cattttgggc atttctacgt caagagtcat cacatcaggt
      721 tcgagctcca atatcttatc tctcgcactg aaagcgtctt gcgcagatcc gaccacttcg
      781 atccccgcat cattggaaag cccccggacg attgcctccc gaaacagcag cgaatcatct
      841 accaccaata cccggatttt tttctttatg ctcattcacg tctatccttt cagataactt
      901 tgcggtaaac tgccggcatt atatattggt atttcgttaa ttcccgatca attgactcgg
      961 aatggccgat aaacaggtaa cctcccggct cagtatgatc atagaatttc tgtaccaact
     1021 catttttggt tttctggtca aaataaatca taacgtttct gcaaaatatg acgtgaaatt
     1081 tttttctgaa tggaaattcg ttttgcatca gattaaaaac ccggaatacg atctcttttt
     1141 tgatcttttc actgaccgcc acattgccgt cgttttgttt ttgaaaataa ttcagtttcc
     1201 aaaatggagg aatatcatta accgcttccg ccggatagat tccggttttg gctttttcca
     1261 atgcgcgcga tgaaatatcc gttgccagta tttcggtatc ccataagtgc ttttcgtcgc
     1321 caaaataatc atgaagaatc atctcaagcg tatacggctc ttcaccggaa gaacatcctg
     1381 cgctccagat tctcatatca tggtcacgaa tattcttcga cagataaggc agcacaactt
     1441 ctttcatata gtcaaaatgc tgtttttcgc gcataaaaaa cgtatggttc gtcgtaagct
     1501 tgttgatcag gtttgatact tctttgccgg ttttatctcc catcgcatag gcgatatatt
     1561 ctccgaagct gctgaagcct ttttctataa caagattgct gagccgtcct tcaatcagcg
     1621 tcttttttcg aaacagattg atcccatagt ttgatttcat aaattgcgca agctgcataa
     1681 attcatcgtc ttttaaaacc agcataaaat ccccctattc taattactcc gtcaaccgat
     1741 tttctgatac acagatccat tgccgaagga tgcctaatcc gcagcggttt tgcagtgcaa
     1801 aattattcgg gccgcttttg aaaaccgatc atgtctttgc accggagagc ggcggcagct
     1861 cccaaacgaa caggattccg gatcaaaaat atcaacagca ttttaatcgc ggcagaagca
     1921 atgaatatta tgcctcatac agccgggcaa aacccggctg tatgaggcaa cagacgacat
     1981 taatacttcc cgaattcttc ctcatcgtcc tctgtcttcg gcggctcggc agaaaccggt
     2041 tctgtctgtt catcttcttt ctgtgcggct gcctcggttt gttcgaggag ctcttcgtga
     2101 gattcttcgg gctcgatcaa ccggtccggc tggtcaaaac tctcagtcac ctcagttaaa
     2161 tcacgctcat cttcatctgt ctggtgaaag ggccttgcag tgatccctct aagtctgaat
     2221 tttccaacca gttcctttag ctgctctgcc tgactgttaa gctcaacact tgccgcggcg
     2281 ctctcctccg ctgtcgagga gttcgtctga accacctggg aaacctgctg gattccttta
     2341 tcgacctgcg agatacctgt ggcttgctca ttcgaggcgc tcgcaatgtc tccgaccagc
     2401 tgggcggcat ggccaattac ttcaacgata ttcttaagct cttcagaagt cgagtcagcg
     2461 atctttgtgc cgttatccac ctgccgaatc gagccttcga tcatttcagc cgtattcttt
     2521 gcggcctctg cgctcttgga tgcaagattc cggacttcct ccgcgacaac cgcgaaccct
     2581 ttgccggcct gcccggccct tgcggcctcg acagcggcat taagcgcaag aatattggtg
     2641 tggaaggcga tatcgtcgat gactttaatt attttaaaga tgtttttgga agactggctg
     2701 atatccacca tcgactgcag catctgatcc atttcacggt tgcccttaac agcattttct
     2761 ctggccgaga tggccagctt atttgcctga ttggcgtttt cggcatttgt tctggtctgg
     2821 acagcgattt gtgaaataga ggcattgagt tcctcaatcg aacttgcctg ggtcgttgct
     2881 ccctccgaaa gctgttggct tccgcttgat acctgagtcg aaccggacgc cacctgctcc
     2941 gcagccgtgt taatgcttcc gagcaactcg ttcagcgaat cgagtatggt attcaacgcg
     3001 gcggatacgg cacggaaatc cccgtcgaaa tcctcaatcc gttcaaaatc aaaattaccc
     3061 atggacatct caatcataat ttccgataca cgatccaggg tctgtctcag actccgggcg
     3121 gttgtgttga ccgcctcgat cagaatttta aactgcccgt tatattcccc ttcgatctct
     3181 acgtcgaaat tggcctgagc gatactggtc atcacttcgg tcacctgact gacaggtttc
     3241 tcaaccgcgg ccagcagatt attcaccgaa tggataatat cggaataccc tccgct
//
LOCUS       DATALY010000228         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_394742,
            whole genome shotgun sequence.
ACCESSION   DATALY010000228 DATALY010000000
VERSION     DATALY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394742"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VHR42_06425"
     CDS             complement(<1..648)
                     /locus_tag="VHR42_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX3026845.1"
                     /translation="MKRTPLEDWILGKTGTADRRMLEQYQLEKIRETLEHVKENSRFY
                     RVYFKSIRPDAIRSFGDFSKVPFTNPNDIRQDSEGFLCVPQREIKRIVTLRSSGTSGE
                     EKRVFFTEEDLELTVDFFQHGMKCLTDRTDRVLVLLPGNSYGSIGDLLKKGLARSDTA
                     CFVAGIMGEPGEAARQIIEHNITCIVGIPMQVLYLSRVERAVFKSRISKVLLSTDY"
     gene            complement(658..2010)
                     /locus_tag="VHR42_06430"
     CDS             complement(658..2010)
                     /locus_tag="VHR42_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX3026846.1"
                     /translation="MKVNPIVFRTQSLCPVCLKKLDATVYERDGGCYLKKTCPEHGVF
                     RSVVWKGSVPIKEWIGKKERAVIKNPLTEVKDGCPYDCGLCSEHRQHTCTALMEVTQR
                     CNLHCRFCFADANAGEYEEPSLEQIKAWYETVKKSAGGCNIQLSGGEPTVRDDLPQII
                     KLGKELGFPFIQINTNGLRIAEDEEYFKALKLAGLDSVFLQFDGTEDSIYKKLRGREL
                     LSIKIKAIENCGKHGVGVVLVPTLVSGVNADNVGKIIDFALEHIRAVRGVHFQPVSYF
                     GRIPQAPRDEDRITLPQLMDELERQTNGRIKAANLKPAKCENPLCSFHGSFLYDPKNR
                     ELTQIAAKISCKCVTQKAEEGAKKTKEYVSQNWTGRESEAARESTGADAGWDRILQSI
                     RSNSFSISAMAFQDAWNVDLERVKDCCIHVVNPRGKLIPFCIYNITNTEGVSLYRNRK
                     "
     gene            complement(2007..2465)
                     /locus_tag="VHR42_06435"
     CDS             complement(2007..2465)
                     /locus_tag="VHR42_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HEX3026847.1"
                     /translation="MDDTGFKMFKLVNCGYCCAQIMVKMALDEEEKENADLMRAVNGL
                     CMGVGSAQKTCGVLTGGIAVLGLYAGKGSDREYPKPGYSEMVDEYTEWFESEFGSTEC
                     RDIIGVCSVEDYNTNQDYRLKCGGTLTKSYEKVQEILREHDFEFGSRDSE"
BASE COUNT          557 a          785 c          623 g          727 t
ORIGIN      
        1 ataatccgta ctgagcagta ccttacttat cctgcttttg aaaaccgccc gttctaccct
       61 gctcaggtac agcacctgca tgggaattcc gacgatgcag gttatgttgt gctcaataat
      121 ctgtcttgcc gcttcgcccg gttctcccat aatcccggcg acaaagcacg cggtatccga
      181 ccttgcgagt ccctttttca gcaaatcgcc gatactgccg taagaattgc ccggaagcaa
      241 aaccagtacc cggtccgtgc ggtcggtcag gcacttcatg ccgtgctgaa aaaaatccac
      301 ggtaagctcc aaatcttcct ccgtaaagaa aacccgcttt tcttccccgc tggtgccgga
      361 gctgcgcagc gtgacaatcc tttttatctc tctctgcggc acacataaaa agccttcgga
      421 atcctgccgg atatcgtttg gattggtgaa aggaaccttt gaaaaatccc caaaggaacg
      481 gattgcatcc gggcgaatac ttttgaaata cacacggtaa aatcggctgt tttcctttac
      541 gtgttccaac gtttcccgga ttttttcaag ctggtattgc tccagcatcc ggcggtctgc
      601 tgttccggtt tttccgagta tccagtcttc cagcggcgtc cgcttcatcg tcatccctca
      661 tttccgattg cggtataagg atactccctc ggtattcgtg atattgtaaa tacagaacgg
      721 aatgagcttc ccccgcgggt ttaccacatg gatgcagcag tcctttaccc gttccaaatc
      781 gacattccac gcatcctgaa acgccatggc ggaaatactg aaggaattgc tgcggatgct
      841 ttgcaaaatc ctgtcccagc ccgcgtccgc gccggtgctt tcccgggccg cttccgactc
      901 cctgccggtc cagttctggg aaacgtattc cttcgttttc ttcgcgccct cttcggcctt
      961 ttgggtcaca catttgcagc tgattttcgc cgcaatctgc gtaagctccc ggtttttcgg
     1021 atcatagaga aaactgccgt ggaaggagca gagcgggttt tcgcatttgg ccggttttag
     1081 attggccgcc ttgattcttc cgttcgtctg ccgttccagt tcgtccataa gctgtggcag
     1141 cgtgatgcgg tcttcgtccc gcggggcctg tgggattcgg ccgaaatagc tgaccggctg
     1201 aaaatgcacc cctcttacgg cgcggatatg ttccagcgcg aaatcgatga tttttccgac
     1261 attgtccgcg ttgactccgg aaaccagtgt gggaaccaga accacgccga cgccgtgttt
     1321 cccgcagttt tcaatcgctt ttatcttgat cgaaaggagt tccctgcccc ggagcttttt
     1381 ataaatcgaa tcttccgtcc cgtcaaactg cagaaaaacc gaatcgagac cggccaactt
     1441 cagcgctttg aaatattcct catcctccgc aatgcgaagg ccgttcgtgt ttatctgaat
     1501 gaacggaaat ccaagctctt tgccaagttt tattatctgc ggcaggtcgt cccttacggt
     1561 cggttctccg cctgaaagct ggatgttgca gccgcccgcg cttttcttta cggtttcgta
     1621 ccatgctttg atctgttcaa gggatggttc ttcgtattct ccggcgtttg catccgcaaa
     1681 gcagaacctg cagtgaaggt tgcagcgctg ggtaacctcc atcagcgccg tgcaggtgtg
     1741 ctgcctgtgt tcgctgcaaa ggccgcagtc gtaggggcac ccgtctttta cctcggtaag
     1801 cggatttttt ataaccgccc gctccttttt ccctatccat tcctttatgg gaacgctgcc
     1861 cttccataca acggagcgga aaacaccgtg ctccgggcag gtctttttta ggtagcatcc
     1921 gccgtcccgc tcatacacag tggcgtcaag ctttttcaga cataccgggc ataggctctg
     1981 cgtgcggaaa acgatcggat tcactttcac tctgaatccc tgcttccgaa ttcaaaatcg
     2041 tgttcccgca ggatttcctg tactttttcg tagcttttgg tcagcgtgcc gccgcatttc
     2101 aggcggtaat cctgattggt gttgtaatcc tccacgctgc aaacgccgat aatatcccgg
     2161 cactccgtgc tgccgaactc gctttcaaac cactccgtgt attcgtccac catttctgag
     2221 tagcctggct tgggatattc ccgatcactg cctttccccg cgtacagccc cagcaccgcg
     2281 attcctcccg taagcacccc acaggttttc tgcgcgctgc ccacgcccat gcagagaccg
     2341 tttaccgccc gcattaaatc tgcgttttcc ttttcttcct cgtccagtgc catttttacc
     2401 atgatctgcg cgcagcagta cccgcagttt acaagcttga acattttaaa tccggtatca
     2461 tccatttttc tttgcctctt ttcttgcgat caggataaaa tatcccggcc tgcagcgttt
     2521 taacgcatcc tgaaagccgc acccgtccat acacccatct gtagaaacat tccagaaggc
     2581 gcccatggag ccgtaggaga atacggcttt caggaagcgt taaaacgctg caggccggga
     2641 tattttatcc tgatcgcaag aaaagaggcg aagaaaaatg gatgataccg ga
//
LOCUS       DATALY010000229        12320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_482255,
            whole genome shotgun sequence.
ACCESSION   DATALY010000229 DATALY010000000
VERSION     DATALY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482255"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VHR42_06440"
     CDS             <1..1213
                     /locus_tag="VHR42_06440"
                     /inference="COORDINATES: protein motif:HMM:NF036810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX3026848.1"
                     /translation="REIRMRADDSVLMLRGNRPYMVRRSRGYAPLPVTCPVRVKKPLL
                     AVGGELKNAFCLVTEDQYYLSPHIGDLGDPRAVETLGGTIDRMSKLLGLKPEAVACDL
                     HPGYRSVEYARQTGLPLVPVQHHFAHVVSCMAENGIQDGESVIGAAFDGTGYGTDGTI
                     WGGEFLKASYSEFERMGSIRPFALAGGDRAAREGYRPAVSLLLQAYNGDAEKVGRIVS
                     RLSLCEEPQLSAQIGMIRNRFNSVMTTSVGRLFDAVAAILGVKRFSTFEGEAASALEY
                     ASAESADGFRFRPVVEQEKLFVLQTGDVIQYITESILEEKSPALLARQFHEAAAAMVA
                     AGCVECRNRTGINTVALSGGVFQNLLLLRLCENMLGEQGFRVLTHSMVPANDGGIALG
                     QAVAAAAGFGR"
     gene            1246..1467
                     /locus_tag="VHR42_06445"
     CDS             1246..1467
                     /locus_tag="VHR42_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX3026849.1"
                     /translation="MCVGLPARVENMDDGMATVSAGGARRKISVELIEGLRPGDYVMV
                     HAGIAIARITEEEAEQTQEILEELYGPQQ"
     gene            1451..2524
                     /gene="hypD"
                     /locus_tag="VHR42_06450"
     CDS             1451..2524
                     /gene="hypD"
                     /locus_tag="VHR42_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX3026850.1"
                     /translation="MARSNNLKDLACRIRNYNGRPLKIMEVCGTHTAQNFKLGIRSLL
                     PEGIRLISGPGCPVCVTPVGYIDRAVELSQIKKVIVCSFGDLLRVPGSKTSLYLERAR
                     GADVRMVYSPLDALEIAVKNPDREIVFLSVGFETTVPASCLTVRKAKERGIKNFSALC
                     SNKTMTRAYYLLKDCADAFLLPGHVSAITGMAVYQRLKADGISGAVAGFTSADLLTAL
                     CAIIEKSASGEPFAVNCYPRVVADEGNPAAMKLIGETMKECDAWWRGIGMIPGSGLML
                     GTDYEEYDARIKFSLGDDRGEENPGCRCGEVLKGTCEPPDCPFYAGKCTPENPLGACM
                     VSSEGTCAAFYKYSGGNESARHS"
     gene            2546..3556
                     /gene="hypE"
                     /locus_tag="VHR42_06455"
     CDS             2546..3556
                     /gene="hypE"
                     /locus_tag="VHR42_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX3026851.1"
                     /translation="MEGIITLAHGAGGTQTNQLIEKIFHQRFQNPYFTSDDAAVLESG
                     GGRLAFTTDGFIVSPPFFPGGDIGKLSVCGTVNDLACMGARPVYLSCSFIIEEGFLIS
                     DLEKIADSMANTAREAGVSIVAGDTKVAGRGQAEGVFITTSGIGFAGPYRLGGALAEE
                     GDAVLVTGDIGRHGSAILCARGDYGIQTDIRSDCAPLSGLAARMLGACGGIHVIRDAT
                     RGGVGTVLYEIAAQSGVCIWVDEETIPVAPEVSGLCGMLGLEPLYMACEGRMVVVVPE
                     RDAGRVLNAIKDGDYGENAAVIGRVTRNSADQPGTVVIETPIGGQRLLARPGGELLPR
                     IC"
     gene            3607..4977
                     /locus_tag="VHR42_06460"
     CDS             3607..4977
                     /locus_tag="VHR42_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3026852.1"
                     /translation="MSAFVSDKGFYKQLAMISIPIALQNLISFGMNMMDTVMLGSLGE
                     NQISASSLANQPFFVFTLFMFGLTSGACVLTAQYWGKGDTHTIGRILMLATKASVGCA
                     LFFSAVVILFPRPVMSIYTQDPAVIELGAQFLRIIGFSYVISAITTTYLFILRSVENV
                     RLPLLINFISFVANTGLNWVLIYGKLGFAPMGIRGSAVATLSARILELIMTLVYAYFF
                     DRQLSFRLGDLFKADFGLVRDFLHYSLPVVLNETIWGLGITLQSVIIGHMGSEEVAAN
                     SIASVVQRLASVVVFGMANAAAVIVGKQIGSGQKEKAAKCAKTILVLSMCFGVASSLL
                     IFLISGPFLSIYHVSETTRIFAMEIMQVFMVTVIFNAFNSVNIVGVLRGGGDTRFAMI
                     IDTGALWLLALPLGALAGLYFKLPLRVVFFLMLIDEPTKFLIGIARFKSGKWLRNVTR
                     EQLPEE"
     gene            5142..5741
                     /locus_tag="VHR42_06465"
     CDS             5142..5741
                     /locus_tag="VHR42_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3026853.1"
                     /translation="MLCPVAVESQAQKDAALVRRIKNGDEDAFQVLLAGYERLIMARA
                     ASYHNAGLEADDLFQEGMIGLISAIRTYNPDGGASFKTYAMLCINRKMVSALRSALSQ
                     KQIPLNSYLSLNDQVLVHDSAFTIYFNPEDMVESREAIGIIKEVMKTHFSSLERRVLN
                     LYVSGLSYAAMAKILNVSTKFIDNALQRIKRKLSLKVSG"
     gene            5851..6129
                     /locus_tag="VHR42_06470"
     CDS             5851..6129
                     /locus_tag="VHR42_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX3026854.1"
                     /translation="MYQDKKLICKECGEEFIFTAGEQEFYAEKGFVNEPQRCKKCRDA
                     RKNAGKPQREMFDAVCASCGKECKVPFQPREDRPVYCSECFAKIKEQA"
     gene            6341..6544
                     /locus_tag="VHR42_06475"
     CDS             6341..6544
                     /locus_tag="VHR42_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX3026855.1"
                     /translation="MAEITKDSLIGEVLDLDQGTAEYFLEMGMHCLGCPASRGESIEE
                     ACMVHGTDSEELVKKINEYLAGK"
     gene            6636..7334
                     /locus_tag="VHR42_06480"
     CDS             6636..7334
                     /locus_tag="VHR42_06480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3026856.1"
                     /translation="MHDCPHLTPRLTLAADFVRPGARLADIGTDHAYLPVYLLKTGRI
                     TRALACDINRGPLGRALATVKKYGVSDRVELRLSDGLDRVAADEAEDIVIAGMGGELI
                     RNIVAHCSWMKDSRRRLILQPMTSAGELREFLVSDGFSIVQEAVAREGNKLYVVICAE
                     YTGSKMLPNEIFKICGTLPRTGGALEREYLLRQAESLFKKADGMRKSRRGLDGETTQA
                     LAESIRAIAEKIKQ"
     gene            7345..8448
                     /locus_tag="VHR42_06485"
     CDS             7345..8448
                     /locus_tag="VHR42_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX3026857.1"
                     /translation="MATVGNIFDYLNRIAPVRMMEEYDNVGLLVGDRNQLVTKVLIAL
                     DITGKVVDKAAAAGAQLIVSHHPVIFRPIRDVRLDGKTGIVYKLANHSLSAVCMHTNL
                     DLANGGVNDCLAQTLELADVEILSVTGKKPMKKITVFVPVDYAAAVRQAMTDAGAGSI
                     QNYRGCAFETQGMSYFRPVEGSNPFIGEVGKVQRVYEMRIEALCPGDRVEEVVRKVIS
                     VHPYEVPVYHVTDDEAMTEPYGIGRVGNLAQPKSLEDFAGFVKQKLFCSAVGVCRSRD
                     DAVRRISVCGGADDGTLVEFSLKAGADTLVIGEMKHSSKIEAKEAGVNIIEAGHFQTE
                     NVICPHLFHILSSQFRDTVFSVANHNCAPDYFI"
     gene            8475..10241
                     /locus_tag="VHR42_06490"
     CDS             8475..10241
                     /locus_tag="VHR42_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEX3026858.1"
                     /translation="MALDGITLSFLIREINRGLESSRVDKILQPEKDEINFVFRQRGS
                     NPRLLLSASASNPRIHFTAIHKENPASPPMFCMLLRKHLSGAKFAGARQNGLDRVAFL
                     DFDTRNEFGDEVRRTIAVEIMGRHSNIILTDENGRILDAVKRVDQTMSSVRQIFPGLP
                     YELPPQQDKLNLAAVSADEIHSRLRAHKDGELSRALMDVLQGVSPIVCRELTGLACRG
                     GRTKLGELTDEDFDRLRFYIGRLAQSVLSGTGRPVMAADVKSGKPLDFSCLEITQYGV
                     GAVTRGFDTFSEMLDSFYSERDRVERVGQRARDILNVITNAAERVSRKLGNQQMELQE
                     CANRGQLKMSGDLISANLYRLEKGAEQCEVENFYDSANPMVVIRLDATLTPAQNAQKY
                     YREYRKATIAETYLNEQICADREELAYLDTVFDELSRASGESELAEIREELESVGYLK
                     ARNRNGRKSRPSKADEPMKFRSTDGFAILAGKNNRQNDRLTLKMAEKRDIWLHTKNIP
                     GSHVIVISNGETVSDQAVTEAAIIAASHSRAGESSQVPVDYTQVRNVKKPSGAKPGMV
                     IYENYKTAFVKPDREIVDRLKV"
     gene            complement(10295..11341)
                     /locus_tag="VHR42_06495"
     CDS             complement(10295..11341)
                     /locus_tag="VHR42_06495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3026859.1"
                     /translation="MKHRAKIEMLAIVVLAFAVLGSCLMLGERTVSTSANTSSGVTGS
                     TIKWVSFNIPYSALNRALQTDISSHTSGKQVHWVELLAYLGTKYGGNWKLYRAKDLDD
                     VAIRLKNGENIQSIAAGMKNYDYFYKAYDAVLGNFVGDYEIQVPDPNNSGSKVFKITY
                     GLKVFSPIAAGYGYGHYDDFGDSRSFGFRRKHLGNDLMGTVGTPIVAVEGGYVESAAW
                     NRYGGWRIGIRSFDKKRYYYYAHLRKDHPFYKNLKQGDIVNSGDVIGYLGMTGYSDKE
                     NINNMKKPHLHFGMQLIFDESQKDGNNEIWIDVYNIVNLLARNRVSVQYSPATKDYSR
                     LYDFFDLKYHLYFD"
     regulatory      11562..11663
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11839..>12320
                     /locus_tag="VHR42_06500"
     CDS             11839..>12320
                     /locus_tag="VHR42_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019391515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX3026860.1"
                     /translation="MKKTPLYDRHVALGGNMIDFGGWMMPVQYSGILNEHERVRNAAG
                     LFDVSHMGEITVRGSGATEYLQKLLTNDIAKAAPGRAVYSPMCYPDGGVVDDLLVYRI
                     SEQSYLVVVNASNTERDYKWFREHLIPGAEIENVSEQYAQIAIQGPAAEAILQQFTVE
                     P"
BASE COUNT         2953 a         2894 c         3476 g         2997 t
ORIGIN      
        1 ccgggaaatc aggatgaggg cggatgattc cgttctcatg cttcgcggga acaggcctta
       61 tatggtgcgc cggtcgaggg gatacgcccc gctgccggta acctgcccgg tgcgggtgaa
      121 gaagcctctc ctggcggtgg gaggcgagct taaaaatgct ttttgccttg tgacggagga
      181 tcagtattat ctttcgcccc atattggcga tctgggagat ccaagggcgg ttgagacgct
      241 tggcgggacc atagacagaa tgtcgaagct gcttggactg aaaccggaag cggtggcctg
      301 cgacctgcat cctggttacc gttcggttga atatgcccgg caaaccggcc tgcctctcgt
      361 cccggttcag catcattttg cccatgtcgt atcctgtatg gcggaaaacg gcatacagga
      421 tggcgaaagc gtcattggcg cggcctttga cggaacggga tacggcaccg atggaaccat
      481 ctggggcgga gaatttctga aggcgtctta ttccgaattt gaacggatgg gcagcatccg
      541 gccatttgcc cttgcgggcg gtgaccgggc ggcgcgggag ggttaccgcc ctgcggtttc
      601 tcttctgctc caggcctata acggcgatgc cgaaaaagtt gggcggattg tttcccggct
      661 gtcgctttgc gaagaacctc agctgtccgc ccaaatcggg atgatccgaa accggttcaa
      721 cagcgtgatg accaccagtg tcggacggct gtttgatgcg gttgccgcca ttcttggggt
      781 caaacggttt tcaacgttcg agggtgaggc ggcctcggct cttgaatatg cgtcggcgga
      841 gtcggcggat ggttttcgct tcagaccggt tgtggaacag gaaaaattat ttgttttgca
      901 gacaggcgat gtgatacaat atattacgga aagcatcctt gaagagaaat ctccggcgct
      961 gcttgcaagg cagtttcacg aagctgcggc ggcgatggtg gcggcgggat gcgtcgaatg
     1021 cagaaaccgg accgggatca atacggtggc tctaagcggc ggggtatttc aaaacctgct
     1081 gcttctcaga ctttgcgaaa acatgcttgg ggagcagggt ttccgggttt tgactcattc
     1141 catggtaccc gccaatgacg gaggaatcgc tcttggtcag gcggtagctg cggcggcggg
     1201 tttcggcagg taaagtttgt tgcttttgta agaataggag gagctatgtg cgtaggattg
     1261 cccgcaaggg ttgaaaatat ggatgatgga atggcaacgg tcagcgcagg cggagcacgg
     1321 aggaaaattt cggttgaatt gatcgaaggg ctcaggcccg gggattatgt catggttcac
     1381 gccggaatcg ccatcgcgcg gattacggag gaagaagccg aacagacaca ggaaattttg
     1441 gaggagcttt atggcccgca gcaataattt gaaagatttg gcctgccgta tccggaatta
     1501 taacggaagg ccgttaaaaa tcatggaggt ttgcggaacg catacggcgc agaatttcaa
     1561 gctgggtata cgcagtctgc tcccggaggg aatccggctg atttccgggc cgggctgccc
     1621 ggtttgtgtt accccggtcg gctatattga tcgggccgtg gaactttcac agatcaaaaa
     1681 agtgattgtc tgctcgtttg gcgacctgct tcgcgttccg ggaagcaaaa cgagtcttta
     1741 tctcgagcga gcccgcggag ccgacgtcag aatggtttac tctccgctcg acgcgcttga
     1801 aattgcggtc aaaaatcccg acagggaaat cgtattcttg tcggtcggat ttgaaaccac
     1861 cgttccggcg tcctgcctta cggtaagaaa ggccaaggag cgtggaatca aaaatttttc
     1921 cgcgctctgt tcaaataaaa ccatgacccg ggcttattat ctgctcaaag actgtgcgga
     1981 cgcatttttg cttcccggtc atgtgagcgc catcacgggc atggcggttt atcaaaggct
     2041 caaagcggac ggtatctcgg gcgccgtcgc gggtttcacc tctgcggact tgctgacggc
     2101 cctctgtgcc attatcgaga aatcagcttc cggagagccc ttcgcggtca actgttatcc
     2161 gagagtggtc gcggatgagg gaaatccggc ggcaatgaag ctgatcgggg aaaccatgaa
     2221 ggaatgtgac gcctggtggc gggggatcgg catgattccc ggttcagggc tgatgcttgg
     2281 aacggattac gaagagtatg acgcaagaat taaattctcg cttggggatg acaggggaga
     2341 agaaaatccg ggctgccgct gcggggaggt tttaaaagga acctgcgagc ctcccgattg
     2401 cccgttttac gccggaaaat gtacgccgga aaatcctctc ggcgcctgta tggtctcctc
     2461 cgaaggaacc tgcgcggcgt tttataaata ttccggcgga aatgagagcg caaggcattc
     2521 ataaaccgta tgcaaggaga atatcatgga gggaattatt acgctcgctc acggagcggg
     2581 tggaactcaa accaatcagc tgattgaaaa gatttttcat cagaggtttc aaaatcctta
     2641 ttttacatcc gatgacgcgg ccgttttaga gagcggaggc ggcaggctgg catttaccac
     2701 cgacggattt atcgtctctc cgcctttttt tccgggaggg gatattggaa agctgagcgt
     2761 ttgtggaacc gtcaacgatt tggcgtgtat gggggcgcgg ccggtttatc tgagctgctc
     2821 ctttatcata gaagaaggat ttctcatctc cgatcttgaa aagattgcgg attctatggc
     2881 gaatactgct cgtgaagcgg gcgtctccat tgttgccggg gacaccaagg ttgccggccg
     2941 cggccaggcc gaaggcgttt ttatcacgac ttcggggatt ggttttgcag gcccgtatcg
     3001 gctgggcggg gcgctggcgg aagaaggcga cgccgttctt gttaccggcg acatcggcag
     3061 acatggaagc gcgatactct gcgccagagg cgattatggg attcagaccg atatccgaag
     3121 tgactgcgct ccgttgtcgg ggcttgccgc gcgaatgctc ggcgcctgcg gcggcatcca
     3181 tgtgatcagg gacgcaaccc gcggcggggt cggcacggtg ctttatgaga tcgctgccca
     3241 gagcggcgtc tgtatctggg tcgacgaaga aaccataccg gttgctccgg aagtttcggg
     3301 actatgcggc atgcttggcc tggaaccgct gtatatggcc tgcgagggca gaatggtggt
     3361 tgtggttcct gagcgggatg cgggaagggt gcttaacgca atcaaagacg gcgactacgg
     3421 tgaaaacgcc gccgttatcg gcagagtgac gcggaacagc gccgaccagc ccggaacggt
     3481 tgtgatcgag acgccgatcg ggggacagag acttctcgcg cggcctggag gcgagctgct
     3541 cccgcggatc tgctgacggc cggatgtttt tctactgact cggcaccaat ggaaaggatg
     3601 aactcgatgt cggcgttcgt atcggacaaa ggcttttaca aacagcttgc tatgatttcc
     3661 attcctatcg ctcttcaaaa cctcattagt ttcggcatga atatgatgga tacggtaatg
     3721 ctcgggtctt tgggcgaaaa ccagatttcc gcatcctcac tggccaatca gccttttttc
     3781 gtgtttactc tgtttatgtt tggcctgaca agcggcgcct gcgtgctgac cgcccaatac
     3841 tggggaaaag gggataccca caccatcggc agaatcttaa tgcttgcgac caaggcgtcg
     3901 gtcggctgcg ctttgttttt ttcggctgtg gtgatcctgt ttccgcggcc tgttatgtcc
     3961 atttacacac aagaccccgc ggtgatcgag ctgggggccc agttcctcag gattatcggt
     4021 ttttcctatg tgatctcggc catcacgacc acctatctgt ttattctgcg aagcgtggaa
     4081 aacgtccgcc ttcctctgct gattaatttt atttcgtttg tcgccaacac cggcctgaac
     4141 tgggtgctga tttatggcaa gctgggtttt gcccctatgg gtatccgagg ctcagctgtc
     4201 gccaccctgt ccgcccgtat tctggaattg atcatgacgc tggtctacgc ctattttttc
     4261 gaccgtcagc tttcgttccg gctgggggac ttgtttaaag ccgatttcgg tcttgtcagg
     4321 gattttctgc attacagctt gcccgtggtg ctcaacgaga ccatctgggg cctcgggatc
     4381 acgcttcagt cggtcattat cggccatatg ggttccgagg aggtggctgc caacagcata
     4441 gccagcgtcg ttcagcggct ggcttcggtg gtggtcttcg gcatggccaa cgctgccgcc
     4501 gtgattgtgg gtaagcaaat cggctcgggt caaaaagaaa aagcggcgaa atgcgccaaa
     4561 accatcctcg ttctgagcat gtgcttcggc gttgcttcct cccttttgat ttttctgatc
     4621 agcgggccgt ttctctcgat ctatcatgtt agcgaaacca ccagaatttt tgccatggaa
     4681 atcatgcagg tttttatggt tacggtgatt ttcaacgcgt ttaattcggt caatattgtg
     4741 ggagtcctcc gcggaggagg agacactcgt ttcgcaatga ttattgatac cggcgcgctt
     4801 tggctgcttg cgcttccgct cggcgccctg gcgggactgt attttaagct tccgctgcgt
     4861 gttgtatttt tcctgatgct aatcgacgaa ccaacgaaat ttttaattgg aatcgcgcga
     4921 tttaaatcgg gaaaatggct gagaaatgtc acaagagagc agcttcccga agaataaaat
     4981 cagatttacc agaaaaaata agaagaaaga aatgaatact ttgaaaaaat caatagaaaa
     5041 agtgctaaaa ttttcagcac aattcttttt tatcttgaca aaaacataaa actattgtat
     5101 tattaatgaa attaaagtag tatggcctgc ggggtgagac aatgttgtgc cctgttgcag
     5161 ttgagtcaca ggcgcaaaaa gatgcggcgc tggtcaggcg gatcaaaaac ggtgatgaag
     5221 atgcgtttca ggttcttttg gcggggtatg agcgtttaat aatggcgcgg gccgccagtt
     5281 atcacaatgc cggtcttgag gcggatgatt tgtttcagga aggcatgatc ggtctgattt
     5341 ccgctattcg aacctataac cctgacggcg gtgcttcgtt taaaacttac gcaatgcttt
     5401 gtataaatag aaaaatggtg tctgccctaa gatctgcttt gagtcaaaaa caaatcccgt
     5461 taaatagtta tctatcctta aacgatcaag ttttggttca tgattctgct tttacaattt
     5521 attttaatcc tgaggatatg gttgaaagcc gcgaagcaat tggaatcatc aaagaagtga
     5581 tgaagactca tttttcatcg cttgagcgca gagttttaaa tttatatgtt tccggcctgt
     5641 cttatgcggc tatggctaaa attcttaatg tcagcacgaa atttatagac aacgcgctgc
     5701 aaagaatcaa acgcaaactg agtcttaaag tttcgggttg agttttttaa gatcatatgc
     5761 cccagtagct caacggaaga gcgttggtat ttcaaaggtg tcggttcaaa tccgtcctcg
     5821 ggcaaatcaa tccaaagcga ggtagaaagc atgtaccagg ataagaagct catttgcaaa
     5881 gaatgcggcg aggaatttat tttcacagcc ggcgaacagg aattttacgc ggaaaaaggg
     5941 ttcgttaacg agcctcagag atgcaaaaag tgccgtgatg ctcgtaagaa cgccggaaag
     6001 ccccagcgtg aaatgttcga cgctgtttgc gcctcttgcg gcaaagagtg caaagttccc
     6061 ttccagccga gggaagaccg ccctgtttac tgcagtgaat gcttcgccaa aattaaggaa
     6121 caagcctaac cggcaccgtt attttggcaa acagacaccc cggaaatttc cggggtgttt
     6181 ttcttgtccg gcaggtattg cttttctaag gaaaggcaat tataatgaat gtagctggag
     6241 cgcaatggct acattcaatc gcttagccat tgtatcgagc ccgatgcggc agaagatcaa
     6301 aagaactgcc tgtcggttaa aaagaaagga acggttcaat atggctgaaa ttacaaaaga
     6361 ttcgttgatc ggtgaagtcc ttgatctaga ccagggaaca gcagagtatt ttcttgaaat
     6421 gggaatgcac tgtctgggct gcccggcttc aagaggcgaa agcatcgaag aagcctgcat
     6481 ggttcacgga acggacagtg aagaactggt caaaaaaatt aatgaatatc ttgccggcaa
     6541 ataaacgata agaacatctt atatgccatc cggcggtgtc ggatggcata tattatttgt
     6601 tgggattcaa cgataaataa ttctgggagg tttccatgca cgactgccca catttgaccc
     6661 ctaggctgac gctggctgcg gattttgtca ggccgggcgc gcgactggcc gatatcggaa
     6721 ccgaccatgc ctatctgccc gtatacctgc ttaaaacggg aagaattacc cgtgcgctgg
     6781 cctgcgatat taaccgaggc ccgcttggcc gcgcgcttgc tacggtgaaa aaatacggtg
     6841 tatccgaccg ggtggaactg cggctgtccg acggtcttga ccgggtcgcg gccgatgaag
     6901 cggaggatat tgtgattgcc gggatgggcg gcgagctgat ccgcaatatt gtcgctcact
     6961 gttcctggat gaaagattcg cgcagacgtc tcattcttca acccatgacg tcggctgggg
     7021 aacttcggga atttcttgta tccgacggat tttccattgt tcaggaggca gtcgcccggg
     7081 aaggcaataa gctctatgtt gtaatttgcg cggaatatac gggaagtaaa atgcttccga
     7141 atgaaatctt taaaatttgc ggaacgcttc cccggacggg cggcgctctt gaacgggaat
     7201 atttactccg acaggcagag agcctgttta aaaaagcgga tggaatgcgg aaatcacggc
     7261 gtggccttga tggggaaacc acgcaggcgc tggcggaatc cattcgggcg attgctgaaa
     7321 aaataaaaca atagggtgat gtgaatggca acggttggaa acatctttga ttatctgaat
     7381 cgtatcgcgc cggtccggat gatggaagaa tatgacaacg tcggtctgct ggtcggcgac
     7441 agaaatcagt tggtcacaaa agtgctgatc gcactggaca ttaccggaaa ggtggtggat
     7501 aaggccgccg ccgctggagc tcagctgatc gtcagccacc atccggtcat attccggcca
     7561 atccgcgatg tccggctgga cggcaagacc ggtattgtct ataaactggc gaatcattcc
     7621 ctgtcggctg tttgcatgca taccaatctt gatttggcaa acggcggagt taatgactgc
     7681 cttgctcaaa cgcttgagtt ggcggatgtt gagatcctga gtgtcacggg caaaaagccg
     7741 atgaaaaaga ttaccgtttt tgtgccggtg gattacgcgg ctgcggtgag gcaggccatg
     7801 acggatgccg gggcgggcag tattcagaat tacaggggat gcgcctttga aacccagggc
     7861 atgagttatt tccggccggt ggaaggctcc aatcccttta tcggggaagt gggcaaagtt
     7921 cagcgggttt acgaaatgcg aattgaagca ctgtgccccg gggacagggt cgaggaagtc
     7981 gtccggaagg tgatttccgt tcatccctat gaggttccgg tttatcatgt gacggatgat
     8041 gaggcgatga cagagcccta cggaatcggc cgggtgggaa accttgccca gcccaaaagc
     8101 ctcgaagatt ttgccggatt tgttaaacaa aaactctttt gcagtgcggt tggagtctgc
     8161 cgaagccgtg atgacgccgt tcgacgcatt tcggtgtgcg gcggggcgga tgacggcaca
     8221 ctcgttgaat tctccttgaa ggcaggggcc gatacccttg tcatcggcga aatgaagcat
     8281 tcttccaaaa ttgaggcaaa agaagcgggc gtcaacataa tcgaagcggg tcattttcaa
     8341 acggaaaatg taatttgccc gcatcttttc catattctat cgagtcagtt tcgggatacg
     8401 gtgttttccg tggcgaatca taactgcgca ccggactatt ttatctaaag attaagaata
     8461 ggtggaagaa gtcaatggct cttgacggca taactttaag ctttttaatc cgggaaatca
     8521 atcggggact ggagtcctcc cgggtcgata aaattctcca gccggaaaag gacgaaatta
     8581 atttcgtttt caggcagcgg gggagcaatc cccgtctgct gctgtcggcc agcgcttcga
     8641 acccaagaat tcattttacc gccattcaca aggaaaatcc tgcttctccc ccaatgtttt
     8701 gtatgctgct ccgcaaacat ctttccgggg caaaatttgc cggagccagg caaaacgggc
     8761 tggacagagt agctttcctg gattttgata cccgcaatga attcggagac gaggtaagaa
     8821 gaaccattgc cgtcgaaatt atggggcggc acagcaatat tatcctcacc gatgaaaacg
     8881 gccggattct tgacgcggtc aaacgggtcg accagacgat gtcgagtgtc aggcagattt
     8941 tccccgggct tccttacgag cttcccccgc agcaggacaa gctgaatctt gctgcggttt
     9001 ccgccgatga gattcacagt cgtctgcgcg ctcataaaga cggggaatta tcccgtgcgc
     9061 tgatggatgt cctgcagggt gtttccccga ttgtctgccg ggaacttacc gggcttgcct
     9121 gccgcggagg acggacgaaa ctgggagaac tgacggatga agactttgat cggctgcgtt
     9181 tttatatcgg gcgattggcg cagtccgttt tatccggaac cggaaggccg gtgatggcgg
     9241 ccgacgttaa gtcgggcaaa cctctggatt tttcctgcct cgagattacc caatacggcg
     9301 ttggcgccgt aacccgcgga tttgatactt tttcagaaat gctcgactcc ttttactctg
     9361 agcgcgacag ggttgaacgc gtcggtcaga gggcccgtga tatcctgaat gtcatcacga
     9421 acgcggcgga acgcgtcagc cggaagcttg gaaaccagca gatggagctc caggagtgcg
     9481 cgaaccgcgg tcagcttaaa atgtccggcg atctgatctc tgcgaacctt taccgtctgg
     9541 aaaagggcgc ggagcaatgc gaagtagaaa atttttatga ttccgcgaat ccaatggtgg
     9601 tgatccggct ggatgccacc ctcacccccg cccagaacgc acagaaatat taccgggaat
     9661 accggaaagc gaccattgcc gaaacttatc tcaacgagca gatctgcgca gaccgggagg
     9721 agctcgctta tcttgacaca gtttttgacg aattaagccg cgcaagcggc gaatcggagc
     9781 tcgccgagat cagggaggag cttgagagcg tcggttatct gaaggcccga aaccggaacg
     9841 gccggaaatc aagaccgtca aaagcagatg agccgatgaa attccgttcc accgacggtt
     9901 ttgcgattct tgccggcaaa aataaccgcc agaacgacag gctgacgctt aaaatggcgg
     9961 aaaagcgtga catctggctg cacaccaaaa atattccggg ttctcatgtg attgtgatat
    10021 caaacggtga gaccgtttca gaccaggccg tcacagaggc ggcgatcatt gccgcgtccc
    10081 acagcagagc cggggaatcg tcccaggttc cggtggatta cacccaggtc aggaatgtca
    10141 aaaagccctc cggcgctaag ccgggaatgg tgatctatga aaattataag actgctttcg
    10201 taaaaccgga ccgggagatt gtcgaccgcc tgaaggtctg acgttgaaaa atccgaagac
    10261 gcagggctcg gaccgtgacg cggtaagtct ccgatcaatc gaaatagaga tggtatttca
    10321 ggtcgaaaaa gtcatagagc cgcgaatagt ctttggttgc ggggctgtat tgtacggaaa
    10381 cccggtttct tgcaagcaaa tttacaatat tataaacgtc gatccagatt tcgttgttgc
    10441 cgtctttctg tgattcgtcg aaaatcagct gcattccaaa atggagatgc ggctttttca
    10501 tgttgttgat gttttcctta tcgctgtatc cggtcattcc gagataaccg atcacgtccc
    10561 ctgaattcac aatgtcgccc tgttttagat ttttatagaa cgggtggtct ttccggagat
    10621 gggcgtagta atagtaccgc tttttgtcaa aactccggat cccgattctc cagcccccgt
    10681 aacggttcca ggccgccgac tccacataac cgccctcaac ggcgacgatg ggggtgccga
    10741 ccgttcccat cagatcgttg cccagatgct tccggcgaaa accgaatgac cgcgaatcgc
    10801 cgaaatcatc atagtgaccg tagccatagc cggcggcaat cggtgagaaa accttcagcc
    10861 cataggtgat tttaaagact tttgatccgc tgttattggg gtcgggcacc tgaatttcat
    10921 aatctccgac gaagttgcct aaaactgcgt catacgcttt ataaaaatag tcgtaatttt
    10981 tcatccctgc cgcgatcgac tgaatgttct ccccgttttt gagccttatg gctacgtcgt
    11041 ccagatcctt tgcccggtaa agtttccagt ttcccccgta ttttgtgccg aggtaagcga
    11101 gaagctccac ccaatggacc tgcttgccgg aggtatggga ggagatgtcg gtttgcaggg
    11161 ctctgttaag agcggaatag gggatgttga aactgaccca tttgatggta ctcccagtga
    11221 caccggaact tgtattggcg ctggtactca cagttcgctc cccgagcatc aggcacgacc
    11281 ccagaaccgc gaacgcaagc acgacgatcg cgagcatttc gatttttgcg cgatgtttca
    11341 accatatcac ctgccgacgc acattttact tttaacacat atatatgtgt taaccatgcg
    11401 tttcattttt gatttttagt ttttaagtga taagacgcga agaatagcat attgacagcc
    11461 gcacccgttc gtactataat caaaccgttg atcaataggg ttaaaaatcg tgctttttcg
    11521 ataaaatcgc aattcatctg tgaaaattga atatggaatg gacgatcggt acgggagaga
    11581 cctttcagca gggcaccgaa gaagtaaact gcaaagcgtc agtttgcagc gaacctttca
    11641 ggtaaacaga ccgtatcagg acataactct gaaaagtcgg ctgatccggc accggaggag
    11701 caataacatg ccgcgggcat ggagaaatct ctcaggttta ataacagaga taagatgaca
    11761 gtttactgtc gttttatctc tgttttttgt tttacagggc atttccatct caaaagcaga
    11821 ggaaagaggg gcggaaccat gaaaaaaaca ccgctgtatg acaggcacgt ggcgctcgga
    11881 ggaaacatga ttgatttcgg gggctggatg atgccggtgc agtattccgg aattctgaac
    11941 gagcatgagc gggtgaggaa tgcggctggg ctttttgacg tatcccatat gggagagatc
    12001 acggtgagag ggagcggtgc gaccgaatac ctccagaaac ttctgaccaa cgatatcgca
    12061 aaagccgcgc cgggccgtgc ggtttattcg cccatgtgtt atcccgacgg gggagtggtg
    12121 gacgatctgc tggtttaccg gatttcggaa cagtcttatc tggtggttgt caacgcatcc
    12181 aacacggaac gcgattacaa atggttccgg gaacatttga ttcccggtgc ggaaattgaa
    12241 aatgtctcgg agcagtacgc acagattgcc attcaggggc ctgcggcgga agccatactt
    12301 cagcaattca ccgttgagcc
//
LOCUS       DATALY010000230         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_655510,
            whole genome shotgun sequence.
ACCESSION   DATALY010000230 DATALY010000000
VERSION     DATALY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655510"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VHR42_06505"
     CDS             <1..1024
                     /locus_tag="VHR42_06505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3026861.1"
                     /translation="KPGIPFMGERYTNDGNYIAQIDGLQARTRERIYNRLASGKIALE
                     EVACPCGGKEFLLLSEKERFGLPVKIVMCKRCGIILQSPRMAQEGFDDFYSGDFSDLA
                     FNINAVSPEDYFFWRKKDGPPVIHEYLKDILTADSHLLEIGCGAGGILDYFRQIGCKV
                     TGIDLDENYISYGRSLGLDLYCCHTRDFISNESRKFDIVILAHVLEHFLDIEKELSDI
                     KKLIMPGGYLYVELPGIKGFSLEYHQFDFLNSLQLAHTYYFDRDTFVQMMSWYGFRCV
                     KSNEYISGLFQIDESVAKRRENHSADLLRFLTYLETHRKSLQKGVFAQETQMKEEAGN
                     GNALGN"
     gene            1002..>1880
                     /locus_tag="VHR42_06510"
     CDS             1002..>1880
                     /locus_tag="VHR42_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3026862.1"
                     /translation="MGMLWETDVYLSEIEKQLDEIDVVSFDIFDTLLLRTVAVPDDIF
                     FKVGKRLQEEYPNTYDFPPEVFAGMRREADAQARKKAWEAEEAEEITLDHIYEQFRLN
                     DEYVACAKACELAVERENVYLNPNMADFISYCHKLGKKLALVSDSYLNRVQITELLST
                     AGLDLSLISTLIISCEYKVLKYNGKLFQILTDAFPDVPKARILHIGDNPHADMDGAAC
                     AGIRSAYYGMIPQTLLHYMSVERNHFGCSPDTVASLRKLAMGSVPHEYRKSEERFFFE
                     AGCSVFGVIYTFFAEWI"
BASE COUNT          489 a          383 c          486 g          522 t
ORIGIN      
        1 aaagccaggt ataccgttta tgggagaacg atataccaac gatggaaatt atattgctca
       61 aattgacggt ttgcaggcgc gaacccgtga acgcatatat aatcgtctgg cctccgggaa
      121 aatcgctttg gaggaagtgg cttgcccatg cggcgggaaa gaatttctgc ttttgagtga
      181 aaaagagcga tttggccttc cggtaaaaat tgtaatgtgc aaacggtgcg gaattattct
      241 ccagagccca aggatggcgc aggaaggctt tgacgatttc tattcgggag acttttccga
      301 tctcgcattt aacattaacg cggtttctcc cgaggattat tttttctggc gtaaaaaaga
      361 tggacctcct gtgattcatg agtacctcaa ggatatttta acggcggaca gccatctgtt
      421 ggaaataggg tgcggagccg gcggcattct ggactatttc aggcagatcg gctgcaaggt
      481 tacaggtata gatctggacg agaattatat ttcatatggc aggtcgctgg gattggattt
      541 gtattgctgc cataccaggg attttatttc aaatgaatcc cgtaaatttg atattgttat
      601 ccttgctcat gtattggagc attttttgga tattgaaaag gaactgtcgg acattaaaaa
      661 gcttatcatg ccaggaggct atctatacgt cgaattgccg ggcataaaag gtttttctct
      721 ggagtatcac cagtttgact tcctgaattc cctgcaactg gcgcatactt attactttga
      781 cagggatact ttcgtgcaga tgatgtcctg gtatgggttc cgctgcgtga aatccaacga
      841 gtacatttcg ggcctgttcc aaattgatga aagcgtcgca aaacggcggg aaaaccatag
      901 cgcagatctg ctcagatttc tgacctatct cgagacgcat cgtaaaagtc tgcaaaaagg
      961 agtatttgcg caggagacac aaatgaagga ggaggcgggc aatgggaatg ctttgggaaa
     1021 ctgacgtata tctgagcgaa atagaaaaac agcttgatga aattgacgtc gtgagcttcg
     1081 atatcttcga tacgctgctt cttcgtaccg ttgccgtgcc ggatgatatt tttttcaaag
     1141 tcggaaagcg gttgcaggaa gagtatccga acacttatga ttttccaccc gaggtttttg
     1201 ccggaatgcg gcgcgaggcg gatgcacagg cccgtaaaaa ggcgtgggag gcggaagaag
     1261 ccgaggaaat cacactggat catatttatg aacagttccg cctgaacgat gaatacgttg
     1321 catgcgcaaa agcatgcgaa ctggctgtgg agcgcgaaaa tgtgtatttg aatccgaaca
     1381 tggcggactt tatcagctat tgtcacaagc tggggaaaaa acttgctctt gtgtcggact
     1441 cttatttgaa cagagtgcag attactgaac ttttatccac agccggttta gatttgtcgt
     1501 tgatcagtac tcttatcata tcatgcgagt acaaggtgct taagtataac ggaaagcttt
     1561 ttcagatttt gacggacgcc tttcccgatg ttcccaaagc ccgtattctg catattggcg
     1621 acaatccgca tgcggacatg gacggagcgg cttgcgccgg tatcaggtcg gcgtattacg
     1681 gcatgattcc gcagacttta ctccattata tgtctgtaga acgcaaccat ttcggctgca
     1741 gtccggatac tgtcgcttca ttgcgtaaat tagccatggg ctccgtcccg cacgagtacc
     1801 gcaaatcgga agaacgtttc ttctttgaag caggttgttc ggtttttggt gttatttata
     1861 ccttctttgc ggaatggatc
//
LOCUS       DATALY010000231         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_394910,
            whole genome shotgun sequence.
ACCESSION   DATALY010000231 DATALY010000000
VERSION     DATALY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394910"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VHR42_06515"
     CDS             <1..678
                     /locus_tag="VHR42_06515"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX3026863.1"
                     /translation="SEIAKEVGAYFMVDMAHIAGLVAAGLHPSPVPYADVVTTTTHKT
                     LRGPRGGMILCKEELAKAIDKAIFPGTQGGPLMHVIAGKAVCFGEALTPAFKEYQKKI
                     VKNAAALAEGLMSRGFDLVSGGTDNHLMLVDLRKFGITGKELEHRLDSVYITVNKNAI
                     PDDPQSPFITSGIRIGTPAVTSRGMEEADMEKIAEFIYLAATEIETKADDIRSGVNEL
                     CSKHPIY"
     gene            756..2042
                     /locus_tag="VHR42_06520"
     CDS             756..2042
                     /locus_tag="VHR42_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX3026864.1"
                     /translation="MSAPLADRLRPKNLEEVVGQSHLLGPGKIIRRIAGSGRIPNMIF
                     YGPSGVGKTTVASIIAGSAGKRLHRLNGTTASTSDIRAIVGELDTFAGMDGVLLYLDE
                     IQYLNKKQQQSLLEFMEDGRITLIASTTENPYFYIYNAILSRSTVFEFKPVEAGETRK
                     AVERAFSCLEDEMGVRFDPEAGVDEALCAACGGDVRRAINAVEMIGLAAKPQDGRCPV
                     TMEMVREVTHSGAARYDRDGDEHYDILSAFQKSVRGSDENAALHYLARLIEAGDLISP
                     CRRLLVMAAEDIGLAYPQAASIVKACVDSAMQLGLPEARIPLAEAVILLCTSPKSNSA
                     ICAIDAAIADLKSGRGGEIPPHLKDAHYQGAGKLGRGTEYRYPHDYPGHWVSQQYLPD
                     EIRDAEYYRYGDNRNERAAKAYREAVKKAADGDQRA"
     gene            2393..>2576
                     /locus_tag="VHR42_06525"
     CDS             2393..>2576
                     /locus_tag="VHR42_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026865.1"
                     /translation="MIEGKDHPLSLEEAAMETACSIVIKKRKKPVKTAKMLHRQLEVI
                     REQYKKLIYSHDQITHA"
BASE COUNT          665 a          622 c          722 g          567 t
ORIGIN      
        1 tccgagattg ccaaagaggt cggagcctat ttcatggttg atatggccca cattgccggt
       61 cttgtggcgg cggggcttca tccctcgccg gtgccgtatg ccgatgtggt gactacaacg
      121 acccacaaaa ccctgcgcgg cccgaggggc ggaatgatcc tgtgtaaaga ggagcttgcc
      181 aaagccatcg acaaggccat ttttccggga acccagggcg gcccgctgat gcatgttatc
      241 gcgggaaaag ccgtttgctt cggcgaagct ctgaccccgg cttttaaaga atatcaaaag
      301 aaaatcgtta aaaacgccgc cgctctggcg gaagggctca tgtcgcgcgg atttgacctg
      361 gtgtccggcg gaacagataa tcacctgatg ctggtcgacc tgcgcaagtt tggaattaca
      421 ggcaaagagc tggagcaccg gcttgacagc gtttatatca ccgtgaataa aaatgcgatt
      481 cccgatgatc cccagagtcc gtttattacc agcggtatcc gtatcggaac acccgcggtt
      541 acaagcaggg gaatggaaga ggcggatatg gaaaaaattg ctgaattcat ctatctggcc
      601 gccacggaaa tcgaaacaaa agcggatgac atccgctccg gcgtcaatga actctgctca
      661 aagcacccga tttattaaat cttaaaaatt tcaggggcag gcgggaagca ccgcctgccc
      721 tttttcgacc ggtaaaaaga aaaggaagtg aagaaatgtc cgctccgctg gcagatcggc
      781 tcaggccaaa aaatctggaa gaagtggtgg gccagtccca tcttctgggc cccgggaaaa
      841 tcatcagaag gattgccggg tcgggcagga tacccaatat gatcttttac gggccgtccg
      901 gtgtcggtaa gacaacggtt gcttcaatca tcgccggctc ggccggaaag agactgcaca
      961 ggctcaacgg aacaaccgct tcaacctccg atatccgtgc gattgtcggt gagctggata
     1021 ctttcgccgg gatggacggc gttctccttt atttggacga aattcaatac ctgaacaaga
     1081 aacagcagca gtcactgctt gagtttatgg aagacggcag aattacgctc atagcctcca
     1141 ctaccgagaa tccgtatttt tatatttata acgccattct cagccggtcg actgtttttg
     1201 agtttaagcc ggtggaagcc ggcgaaaccc gcaaagcggt ggagcgggcg ttttcctgcc
     1261 tggaggatga gatgggcgtc cgctttgatc ctgaagccgg agtggatgaa gcattgtgcg
     1321 ctgcatgcgg cggagatgtc cgcagggcga ttaacgcggt ggaaatgatt gggcttgccg
     1381 caaaaccgca ggacggccgg tgcccggtca caatggaaat ggtccgggag gtcacacaca
     1441 gcggagctgc ccgctatgat cgggatggcg atgagcatta cgacatcctc tcggcctttc
     1501 agaaatcggt caggggttcg gatgagaacg ccgcccttca ttatcttgcg cgcctgattg
     1561 aagcgggaga tcttatttct ccctgccgac ggcttctggt gatggcggcg gaggatatcg
     1621 gccttgccta tccgcaggcg gcgtccattg tcaaagcctg tgtggattcg gcaatgcagc
     1681 ttggtctgcc ggaagcgcgt atcccgctcg ccgaggccgt gattctgctt tgcacttctc
     1741 caaaatccaa ctcggcgatc tgtgcgatcg acgcggcaat tgccgacttg aagtccggcc
     1801 ggggaggaga aattccgccg catctcaaag acgcccatta tcagggcgcg ggaaaactgg
     1861 ggaggggaac ggagtatcgc tatccccatg attatcccgg tcactgggtc agccagcagt
     1921 atttgccgga tgaaatcagg gacgccgaat actaccgata tggagacaat cggaatgagc
     1981 gggcggcgaa agcgtatcgt gaggctgtta aaaaagcagc tgacggcgat cagcgtgcgt
     2041 aaaacaggca aaaccgattc agctgggagc ggttccgtat ggagccgctc ccagctctgt
     2101 attacacgaa gtgcagttgc cgtgcaaacc gataatacac cggtctcgaa gatgattttt
     2161 tcgagaatta ttcggcgtat cggttcggac ttgtatgaca ataagaaaaa taaaacggca
     2221 aagaagggct tggcgctgtc gttttataaa ttgcgggatt ttacagcaag agtaaacgcg
     2281 cggatgatgc tcaggaacat ttttcaaaac aagacgcatg cttttgttat cagtgggaaa
     2341 aaatattgta gaacttaaat tttccccata ggaataaagg caggtttatt gaatgattga
     2401 aggcaaggat catccgcttt ctttggaaga agctgcgatg gaaaccgcgt gttccattgt
     2461 tataaaaaag agaaaaaaac ccgttaaaac agcgaaaatg cttcaccgtc agctggaagt
     2521 tatccgcgaa caatataaaa agctgattta ttcacacgat caaattaccc atgcat
//
LOCUS       DATALY010000232         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_567417,
            whole genome shotgun sequence.
ACCESSION   DATALY010000232 DATALY010000000
VERSION     DATALY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567417"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VHR42_06530"
     CDS             <1..1084
                     /locus_tag="VHR42_06530"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3026866.1"
                     /translation="SGNASAKQTSSASAGNTEKSDNIVVWGEVKYNDEYQINIDFPST
                     VQNILVKEGDTVKKGTPLIALSTDDYQENIKKLQTQVHSSQAALDSVNQAAMEAEIAT
                     LKKQIANKTAELNSGSKAELQLLQNSLVRTDKEMKDARNDLSKYQKLYNDGVIAKADL
                     DKYADIVNQKEKARNDTVDNIAKTKRTLQEELDTMNTSLKYKEVQLSQQKDLASAAQN
                     DLNIMTRKANKPYLSGNTVISNLDYGIVKEVSVVKGSALGTQYAAQKAISLIDGSSIY
                     VSAEVPEEFIRQISAESKAEIIPAADKNLKISGQIVRIANDAVEKDGERIVKVQIKPD
                     DKENILKPGYSADVHITRTGKSGRNG"
     gene            1146..1610
                     /locus_tag="VHR42_06535"
     CDS             1146..1610
                     /locus_tag="VHR42_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3026867.1"
                     /translation="MRITKKPEERKQEIVEAALELFLQKGYDNVTVKDITTKVNVAAG
                     LFHYYFHSKEDVLLECVRLDRKNFFDALTRSNCFPAELMAQAKHRDFSGLSGIFTGKN
                     AFLIALNNKFRHFIRNCAENLVFNLIYQHGRINTPTVFKQLRARRDIMQNLA"
BASE COUNT          539 a          401 c          407 g          352 t
ORIGIN      
        1 cagcgggaat gcttccgcga agcagacgtc ttcggcttcc gcagggaata ccgaaaaatc
       61 ggacaatatc gtcgtttggg gcgaagtaaa atacaacgac gaataccaga ttaacattga
      121 ttttccgtca accgtgcaga atatcctcgt gaaggaaggt gacaccgtaa agaaaggcac
      181 cccgcttatc gcactttcca ccgacgacta tcaggaaaac atcaaaaaac tgcagacaca
      241 ggttcattcc agtcaggcgg cgctggacag cgtgaatcag gccgcgatgg aagcagaaat
      301 tgccacgtta aaaaagcaga ttgcaaacaa aacggccgaa ctgaacagcg gcagcaaggc
      361 ggaactgcag cttctgcaaa attctctcgt gcgaacagac aaagaaatga aagacgcacg
      421 gaatgacctc agtaaatatc agaaacttta taacgacggc gtaattgcga aggctgacct
      481 ggataagtat gcggatattg taaaccaaaa ggaaaaagcg agaaatgaca ctgtggataa
      541 cattgccaaa acgaagcgca ctctgcagga ggagctggat accatgaaca cctcgctgaa
      601 atacaaagag gttcagctga gtcagcagaa ggacctcgcc tccgctgccc aaaatgatct
      661 gaacatcatg acgcgaaaag ccaataagcc ctatctttcc ggaaataccg tcatatccaa
      721 tctagattac ggcatcgtta aggaagtcag cgtcgtaaag ggctctgcgc ttggaacaca
      781 gtacgcggcg caaaaagcaa tcagcctgat cgatggcagc agcatatacg tcagcgcgga
      841 agttcccgag gaatttatcc ggcagatttc cgccgagagc aaggcggaaa ttatccccgc
      901 tgccgacaag aatctgaaaa tatccgggca aatcgtgcgc atcgccaacg acgcggtgga
      961 aaaggacggg gaacgtattg taaaagtcca gatcaagccc gatgataagg aaaatattct
     1021 caagccgggc tacagcgcgg atgtgcatat aaccagaacc ggcaaatccg ggcggaacgg
     1081 ttgacagaaa agcctttcct gctacaatta ggataatacc gaataaagca tgggagtgcg
     1141 ataacatgag aatcaccaaa aagccggagg aacgcaaaca ggaaatcgtg gaagcagcac
     1201 tggagctgtt ccttcaaaag ggatatgata atgtcaccgt caaagatatc actacaaaag
     1261 taaacgttgc ggccggcttg ttccattatt attttcattc caaggaagac gtacttcttg
     1321 agtgcgtacg gctggaccgg aagaattttt ttgatgcatt gaccagatca aactgcttcc
     1381 cggctgaact gatggctcag gccaaacacc gcgatttcag cggtctctct gggatattta
     1441 caggcaaaaa cgccttcctg attgccctga ataataaatt ccgtcatttt atccgcaatt
     1501 gcgccgagaa cctcgtattt aacctgattt accagcatgg cagaattaat actccaactg
     1561 tctttaaaca gctgcgtgcg cgccgcgata ttatgcagaa tcttgcttag cagaagatta
     1621 attttatcga tggcacacat atcttccggg aagcagtttg atttggtcag tgcatcaaaa
     1681 aatttcttcc ggtccagcc
//
LOCUS       DATALY010000233         4588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_225882,
            whole genome shotgun sequence.
ACCESSION   DATALY010000233 DATALY010000000
VERSION     DATALY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225882"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VHR42_06540"
     CDS             complement(<1..1170)
                     /locus_tag="VHR42_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEX3026868.1"
                     /translation="MYYAHTKFNPVTGENLSPDYWQPLLEHLQNVAAIAQQFGDSFGS
                     GEIARICGLFHDLGKYSERFQRHLSEGGNPVDHSTAGAVELSKRLDSLGFLLAYCVAG
                     HHAGLPDGGSAADTPDEPTLHARLRKKTESYDGYRQELDPLSQPYDLRLPIAPLGQGG
                     FSLSFYIRMLYSCLVDGDFLDTEKAMNGEIDRKIGEEISTLAVRLEQYIGRFDNSDRP
                     IDRMRTQILKSCVAKAAWDKGLYTLTVPTGSGKTISSLAFALKHANFHRMKRVIYVIP
                     YNSIIEQNAAVFKDILGEENVLEHHSNFNYDDSSEKLIRQRLATENWDMPVIVTTTVQ
                     FFESIFANRSSKCRKLHNLADSVVIFDDGVIRDHVNNPFHPVKVGVFKGERQRGDC"
     gene            1599..1823
                     /locus_tag="VHR42_06545"
     CDS             1599..1823
                     /locus_tag="VHR42_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026869.1"
                     /translation="MDAVQTELIRVLLKCVYEQGLISEDTYSSALEKVSIAVDGGTPS
                     RYNVLPQKGNIQVKNDCREGNAIGCAQGKG"
     gene            1804..3411
                     /locus_tag="VHR42_06550"
     CDS             1804..3411
                     /locus_tag="VHR42_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3026870.1"
                     /translation="MRKVRAEIMGGRSVYDLPLRVTFYARVSTDKEEQASSLENQVAY
                     YSDLIRKSKNWTYVDGYVDEGISGTSTLKRESFNRMILDAKRGLFDFIITKEISRFSR
                     STLDSIQYTQELLRNNVGVFFQSDNINTLDTDSEFRLVVMAGVAQDEVRKLSERLKFG
                     FRQSIRNGRVLGNDRLWGYDKKDCKLTINEEEAKIIRLIFELYVNQRIGMRKISQKLL
                     ERGVTSREGNAFNTTTIRHILNNPKYKGYYCGNKTKSIDYKLKKNVELDESEWVCYQD
                     EDIPAIVSEGLWDKAHAQLQNRSKALRSSGAEFSNRYPYSGKIICAEHGTSFHRQVLH
                     SQKGDKEIWQCRTYRTRGLVGCSAPQLRTAEIDRIMADIFSQVNKDKDQIINKMISLI
                     KSSSNQRDYGREKEKLEEEIRGIYAKKDKLLEMSIASFITNAEFKTRNDDFNRRLDLK
                     QKSMEQLSAEQEKSRSETENFGKIKEVLTQELNFENGIKSELVAAILDRIVVKNTGDR
                     NHIRFDVHLKMGEKIPVEYDRKKSILL"
     gene            complement(3720..4046)
                     /locus_tag="VHR42_06555"
     CDS             complement(3720..4046)
                     /locus_tag="VHR42_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026871.1"
                     /translation="MKVKLVSTLSALLIMAVALTAAFALPRSQKLMQKSSSVSTVSAK
                     KVTGYIVKEYHGKVGIFLTGKNTPQQILDVYVSSLPQPDQGELKTGISVATQQKLLEM
                     IENYTS"
     gene            4183..>4588
                     /locus_tag="VHR42_06560"
     CDS             4183..>4588
                     /locus_tag="VHR42_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX3026872.1"
                     /translation="MDKYKVVKQQVKMKKKEITPIKRKFRSVGKVLLTIFLIGVITGC
                     LVFGGMVFYVINFVKPDNIDLNREKLDYTSIVYGQNSSGQTVEVDRLSSQANRIWVDI
                     DQVPKQLKDAVVSIEDQRFYEHEGVDWKRTIGA"
BASE COUNT         1268 a         1047 c         1165 g         1108 t
ORIGIN      
        1 acaatctcct ctctggcgtt cgcccttaaa cacgccaact ttcaccggat gaaacgggtt
       61 atttacgtga tcccttataa ctccatcgtc aaaaataacg acgctgtcag ccaaattgtg
      121 cagcttacgg catttggagg atcggttggc aaagatcgac tcaaaaaact gtacggttgt
      181 ggtaacaatg acaggcatgt cccagttctc ggtggcaagc cgctgccgga tgagtttttc
      241 gcttgaatcg tcataattga aattggaatg gtgctccagc acgttttctt cgcccaatat
      301 gtctttaaaa acggcggcgt tctgctcgat gatggagtta taagggatca cgtaaataac
      361 ccgtttcatc cggtgaaagt tggcgtgttt aagggcgaac gccagagagg agattgtctt
      421 gccgctgccc gtcggcaccg tgagagtata caggccctta tcccaggcgg cttttgccac
      481 acaacttttc agaatctgcg tccgcatccg atcgatcggc ctgtcggaat tgtcgaagcg
      541 ccctatgtat tgctcgagtc tgacggcaag ggtggaaatc tcttcaccga tttttcggtc
      601 gatctctccg ttcatggctt tttcggtatc aagaaaatcg ccgtcgacca ggcaggagta
      661 gagcatgcgg atatagaagg aaagagagaa cccaccttgc ccgagcgggg cgatcggaag
      721 gcgcagatca tacggctgtg agagtgggtc taattcttgc cggtagccgt catagctctc
      781 ggtttttttg cgaagccgcg catgcagcgt cggctcatcc ggggtatcgg cggccgagcc
      841 gccgtccggc aggccggcat gatggcctgc gacgcaataa gcaagcaaaa agcctaagct
      901 gtcaagccgt ttgctaagct ccacagcgcc cgctgtagag tggtcaaccg ggttgccgcc
      961 ttcagaaaga tgcctctgaa atcgttcgga atacttacct aggtcgtgga acaacccgca
     1021 gatccgggcg atttcaccgc tcccgaaact gtctccgaac tgctgtgcaa tcgccgccac
     1081 attttgaaga tgctccaaaa gaggctgcca ataatccggg ctcaaatttt ctccggtaac
     1141 cggattgaat ttggtatgag cataatacat ttttgccctt ctttcccata acttatcaga
     1201 caatcctatt ttaggagtta tggaacaaaa agtaaactct atccttttat tatggttgac
     1261 aatgcccgga taccgaatgc gcttatccgg cagcaactca gacgcatcac ccgcaatggt
     1321 aagccattcc tgatagcggt cgcaggctca aattcacaaa aaatattttt tagagtattc
     1381 cccctcgttt tgcatgtttg catgcatggg ggccgctctt tcggagaaag agcggtgaga
     1441 tctatgacac ctatgtcgca gatggtttgc agcgacggct ttttcaacac aaatttctgc
     1501 ttctgttgaa agcggaagag agccaatggt ttttgattcc aacataagtg gacggcagcg
     1561 ctcataaatt ttaagatggg gaaggaggat ggtgccggat ggatgctgtg cagaccgaac
     1621 tgattcgtgt tcttctcaaa tgtgtttacg agcaggggct catcagtgaa gacacctatt
     1681 cctccgctct tgaaaaagtc tcgattgccg ttgacggtgg cacgccttcc cgctataacg
     1741 tcttaccaca aaagggtaat attcaggtaa aaaatgattg tcgggaggga aacgcgattg
     1801 gatgtgcgca aggtaagggc tgaaatcatg ggtgggcgat ccgtttatga tttgccgctg
     1861 cgggtcactt tttatgcgag ggtgtccacc gacaaggagg aacaggcaag ctcgcttgaa
     1921 aaccaagtcg cctattattc ggacttaata agaaaaagca agaactggac atatgttgac
     1981 ggctatgtgg atgaaggcat cagcgggaca agtacattaa aacgggaaag tttcaatcga
     2041 atgatcctcg atgccaaaag agggttgttc gattttatta ttacgaagga aatttcgcgc
     2101 ttttcccgca gcacccttga cagtattcaa tacacacagg agcttttgcg taacaatgta
     2161 ggtgtatttt ttcagtcgga caatatcaat acccttgata ccgacagcga gttccgactt
     2221 gtcgttatgg ccggcgtcgc gcaagacgag gtgcgcaagc tgtcggaacg gctcaaattc
     2281 ggcttccggc agtcgattag aaacgggcgt gttctgggaa acgaccgcct gtggggatac
     2341 gataaaaaag actgcaaact gaccatcaac gaagaggaag cgaagattat ccgcctgatt
     2401 tttgagctct atgtaaatca gcggatcggt atgcgcaaga tttcacaaaa gcttctggaa
     2461 cgcggtgtca ccagccggga gggcaatgcc tttaatacga caaccatccg gcatatcctc
     2521 aacaacccaa aatacaaagg ctattactgc ggcaacaaga caaaatccat tgattataaa
     2581 ctgaaaaaga atgtcgagct ggatgaaagc gaatgggtgt gttatcagga cgaggacatt
     2641 ccggccattg tttcagaagg attgtgggac aaggcacatg cgcagctgca aaaccgcagc
     2701 aaggcactgc gcagcagcgg cgccgaattc agcaatcgct atccttacag cggaaaaatc
     2761 atctgcgcag aacacggcac gagctttcat cggcaggtgc tccactccca aaaaggtgat
     2821 aaggagatct ggcagtgccg cacctaccgt acgcgcgggc ttgtgggctg ctccgcgccg
     2881 caactgcgta ccgccgagat tgaccgcatc atggcggata ttttttcaca ggtgaacaag
     2941 gacaaagacc agattatcaa taaaatgatc tcactcatca aaagctcctc aaatcagcgg
     3001 gattacggaa gggaaaaaga aaagctggag gaggagatac gcggtattta cgcgaaaaag
     3061 gataagctgc tggagatgag catcgcctct ttcattacca acgccgagtt caaaacccgc
     3121 aacgacgatt tcaaccgccg gcttgactta aagcaaaaga gcatggagca gctatcggca
     3181 gagcaggaga aaagccgatc cgaaactgaa aattttggta aaatcaagga agtgctcacg
     3241 caggaactga actttgaaaa cggcatcaaa tcagagcttg tggccgcgat ccttgacaga
     3301 atcgttgtga aaaacacagg cgaccgcaat catatcagat tcgatgttca cctcaaaatg
     3361 ggcgaaaaaa taccggtcga atacgaccgg aaaaaatcta tccttttgtg acaatacttc
     3421 ttaatgatat gtgcaaccaa tctgagattt ctctcgatca acacgtttct ggcggtgggg
     3481 tccccttctt tgagcagagt aagataatgc cgctcttccg ttgcggaaag cggccgggga
     3541 aaggaaccgg aattggtgac atgcagaatc aggtaaatca ggttggcagc ggcgaatccg
     3601 agaaaagaga acacggcggc acctccccta ggctgataca tcaggtatca caccatttat
     3661 tatatggggg atgccgcccc gctgtgcaag tccccgccgc aggaattaaa aagatcgaat
     3721 caactggtat aattttcgat catttcaaga agtttttgct gggtggcaac ggagataccg
     3781 gttttcagct ctccctgatc cggctgggga agacttgaga cgtaaacgtc caatatctgc
     3841 tgcggagtat tctttccggt caggaaaatg cccacttttc catgatattc tttcacgata
     3901 tagccggtga cttttttcgc cgacacagtt gaaaccgaag aggatttttg catcagtttc
     3961 tggctccgcg gcagcgcgaa cgcagccgtc agggcaaccg ccataatcag cagcgccgag
     4021 agagtcgaaa caagttttac tttcattttt ataacgcctc caaaaactat tttgcccgct
     4081 ttttccctga ttatgtattg taaattttag atttttatat agacttgaga aaattgtggt
     4141 ataatagtaa agattatata ggtaaggagg cgttgccgct tcatggataa atataaagtt
     4201 gtgaaacaac aggtcaagat gaaaaagaag gaaatcaccc cgatcaagag aaaattcagg
     4261 tcggtcggaa aagtattgct aaccattttt ctgatcggcg tcattaccgg atgtcttgta
     4321 tttggcggga tggtttttta tgtgattaat tttgtaaagc ctgataatat tgacttaaac
     4381 agggagaagc ttgattacac gtcgattgtt tacgggcaga atagttcagg tcagacggtg
     4441 gaagtcgacc ggttaagcag tcaggcaaac aggatctggg tggatatcga tcaggtaccc
     4501 aaacagctca aagacgctgt cgtcagtatc gaggatcaac gtttctacga gcacgagggt
     4561 gtagattgga agcgaaccat aggagcat
//
LOCUS       DATALY010000234         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_137431,
            whole genome shotgun sequence.
ACCESSION   DATALY010000234 DATALY010000000
VERSION     DATALY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137431"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="whiA"
                     /locus_tag="VHR42_06565"
     CDS             complement(<1..677)
                     /gene="whiA"
                     /locus_tag="VHR42_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX3026873.1"
                     /translation="MCCKKASLYGLLLFARSFSPTGITLQTENQDVVSLVMELLRELF
                     RIVPAFKTARRSDGHRTNILTLDDPNSAKIIYESFGYDQRTVALRINRANIENECCSA
                     SFLRGVFLSCGSIVDPGKDYHLEFASPHMQLAKDLMAYLSELDFHPKMVGRQGGSVIY
                     FKESEQIEDMLTIMGAVRQSLELMNVKVYKDLRNKVNRVTNCETANIGKTVAASSEQV
                     EAIKKIL"
     gene            complement(753..1610)
                     /gene="rapZ"
                     /locus_tag="VHR42_06570"
     CDS             complement(753..1610)
                     /gene="rapZ"
                     /locus_tag="VHR42_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX3026874.1"
                     /translation="MAFVIITGLSGAGKSRAVNALEDIGFYCVDNMPPTLIPKFADLC
                     LKTEGKIENIAIVTDVRGGALFNSLFDGLDELKKQGGDYKILFLDADDQVLVRRFKET
                     RRKHPLSELGSTIGESLRQEREILRPARERADYIIDTTNLSPAQLKERITVLFLGDNK
                     TGLIVNCMSFGFKYGAPAEGDLVFDVRCFSNPFYVDELKQLTGLDEPVKDYVFASPKT
                     QGFVTRLFDMIDYLTPLYADEGKSQLVVAIGCTGGKHRSVAIAEALYKHLLEKGIRTA
                     VNHRDIQKP"
     gene            complement(1627..2460)
                     /gene="murB"
                     /locus_tag="VHR42_06575"
     CDS             complement(1627..2460)
                     /gene="murB"
                     /locus_tag="VHR42_06575"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX3026875.1"
                     /translation="MSRHTTFKIGGAARIFIIPADVEAFRKIFAFCSGREIPCIVIGN
                     GSNLLISDRGLDCAVLRLDQEMGGIDLDGDGVVCEAGAPLKTVCAFARDQGLTGLEFA
                     FGIPGTVGGAVFMNAGAYGGEMSRVIDWTEHLGVSGPGKYTGAHPGYGYRISPYTGGG
                     FAVTRVRFSLKPGDREEIDGRMKELLERRRTKQPLEYPSAGSVFKRPPGYFAGTLIEN
                     CGLKGLRVGGAAVSQKHAGFIINLGGATCADVCCLIDKIKNEVFQKTGVELESEIKVL
                     G"
     gene            complement(2578..3531)
                     /locus_tag="VHR42_06580"
     CDS             complement(2578..3531)
                     /locus_tag="VHR42_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3026876.1"
                     /translation="MYYLGVDLGGTNIKAGLVDESMRLVLKESTPTGEGSGAVEIVKR
                     IADLCRKIVRRAEVSMDDVAWVGIGAPGSADTVRGVLEYANNLPFDNVPLAQMLKDEI
                     GKNVLIENDANAAAYGEFMAGSAKSSKSAVLITLGTGVGSGIIIDGRILQGHNFAGGE
                     CGHTVIEIDGRECTCGRKGCWEAYSSATGLIHMAQEAMTASPGSKMWKIAGGELSRVD
                     GRTPFDAMKAGDEAGKQVVEEYIRYLACGLVNVINIFQPEILCIGGGVSNEGDVLIRP
                     LRKMIEKERYSRYSAHQTELRAAQLGNDAGIIGAAMLGRQG"
     gene            complement(3555..4313)
                     /locus_tag="VHR42_06585"
     CDS             complement(3555..4313)
                     /locus_tag="VHR42_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX3026877.1"
                     /translation="MSIKPECQVPVEADTHCHTLASSHAYSTVAELARYAAAKGLKAI
                     AVTDHGPELTDGCHLWHFSSLKSLPPMIEGVRVLHGAEANIQGFDGKIDIPERITRQL
                     DWVIASYHEPVTSPGTADEHTQSYLKLAENPFVDVIGHSGSDKYRYDYEKAIPVFKKY
                     GKLVELNSHSFEARKGAKKNCREIALICKKHLVPVVVNSDAHSCFAVGEVTEAFKMLK
                     EIDFPEDLIVNLKYDRLMEWICRKRKRLSAIPER"
     gene            complement(4303..5265)
                     /gene="hprK"
                     /locus_tag="VHR42_06590"
     CDS             complement(4303..5265)
                     /gene="hprK"
                     /locus_tag="VHR42_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX3026878.1"
                     /translation="MDGRFTVSLEKIIREFSLESIYLPEDPDRIMISTADVNRPGLQL
                     GGFFDYFDKSRIQIIGLTEISFLKRFVEEKRRSAVDRLFSYQPPAFVICRGIELFDEM
                     AELAKAYKVPLLRTSEPTSGFMSGLISYLNVELAPRITRHGVLVEVYGEGILLLGESG
                     VGKSETAIELVKRGHRLIADDAVEIRRVSSKTLVGSSPENIRHFVELRGVGIVNARRI
                     FGMGAVKVTEKVDMIIHLEVWDSNKVYDRMGMENEHTEILGINVPSLTIPVKPGRNLA
                     IIIEVAAMNNRQKKMGYNAAQELLEKLGLAEPQEQTKEVDWDVY"
BASE COUNT         1212 a         1581 c         1280 g         1322 t
ORIGIN      
        1 ttcaatatct ttttgatggc ctcgacctgc tcggaggagg ccgcgacggt cttgccgatg
       61 ttggccgtct cacagttcgt cacacgattg accttgttgc gcagatcttt gtaaaccttg
      121 acattcatca gttcgagcga ctgccgcacc gcgcccatga tcgtcagcat atcttcaatt
      181 tgttcgctct ctttgaaata gattacactc ccaccctgcc tgccgaccat tttcgggtgg
      241 aaatcaagct cgctgagata agccatgaga tccttggcca gctgcatatg gggcgaggca
      301 aattccagat ggtaatcctt ccccgggtcg acgatcgagc cgcatgacaa aaatactcct
      361 cttaaaaacg atgcggagca gcattcgttt tcgatattgg cccggttgat gcgcagcgcg
      421 acggtgcgtt ggtcatagcc gaaactttca tagatgattt tggcggaatt cggatcatcc
      481 agagtaagaa tgttcgttct gtggccgtcg gacctgcggg cggttttaaa tgccggcaca
      541 attcggaaaa gctccctcag gagctccatg acaagagaaa caacatcctg attctccgtc
      601 tgaagggtaa ttcccgtcgg ggaaaaggag cgcgcaaaca gcaaaaggcc ataaagggag
      661 gcttttttgc agcacatccc ttccggcctt atagaacaca gctctgattt tacttcatgt
      721 gcgaaagaca gaggcaagtc gatcaccttt tcttacggct tttgtatgtc acgatgattg
      781 acggctgtcc ggatgccctt ttcgagcaaa tgcttgtaaa gggcctcggc aatcgccacc
      841 gaacgatgtt ttcccccggt gcagccgatg gccacaacca gctggctctt accttcgtcc
      901 gcatagagcg gtgtcagata atcaatcata tcaaacagcc tggtcacaaa gccctgcgtt
      961 ttcggagagg cgaaaacata atctttgacc ggctcgtcaa gccccgtcag ctgtttgagt
     1021 tcgtcgacat agaaagggtt ggaaaaacac cggacgtcaa ataccagatc gccttcggcc
     1081 ggcgcgccat atttaaagcc gaacgacatg cagttgacga tcagacccgt tttgttgtcg
     1141 ccgagaaaaa gcaccgtgat ccgctctttg agctgggcgg gagacaggtt ggtggtgtcg
     1201 ataatataat cggcccgctc ccttgccggc cggagaatct cacgctcctg ccggagcgat
     1261 tcgccgatgg tactcccaag ctcggataac ggatgctttc gccgcgtttc tttaaaacgc
     1321 cggaccagaa cctgatcgtc ggcatccaga aacaggattt tatagtctcc cccctgcttt
     1381 ttcagctcgt ccagcccgtc aaacaggcta ttgaacagcg cgccgccgcg cacatcggta
     1441 acaatcgcga tattttcgat ttttccctcg gttttcagac acagatcggc aaattttggt
     1501 atcagagtcg gaggcatgtt atccacgcaa tagaagccga tatcttccaa ggcgttgact
     1561 gcccttgact tgcctgcgcc agacaaaccg gtgatgatta caaatgccat tttgaacctc
     1621 cagattttac cccagcactt tgatctcgct ttcgagttcc actccagttt tttggaatac
     1681 ctcgtttttt atcttatcaa tcaagcagca gacgtcagca caggtcgctc ccccaaggtt
     1741 tataataaaa cccgcgtgct tttggcttac tgccgctccg ccgacgcgca ggcccttcag
     1801 cccgcagttt tcaatcagcg taccggcaaa atatcccggc ggccttttaa agacgctgcc
     1861 cgcgctggga tattccagcg gctgctttgt tcttcgcctt tcgagaagtt ccttcatcct
     1921 gccgtcaatt tcctcccggt ctcccggctt taaagaaaac cgcacccggg taaccgcgaa
     1981 gccgcccccg gtataagggc tgatccggta accgtaaccg gggtgcgcgc ccgtatattt
     2041 tccgggtccg ctgacgccca gatgttccgt ccagtcaata acccggctca tttcaccgcc
     2101 gtaagccccc gcgttcataa aaacagcgcc gcctacggtt ccgggtattc caaaggcaaa
     2161 ttcgagcccc gtcaggccct gatcgcgggc gaatgcgcaa accgttttca ggggcgcccc
     2221 cgcctcacag accacaccgt ccccgtcgag gtcgattccg cccatttcct gatcgagccg
     2281 gagaaccgca cagtcgagcc cccgatcgga aatcagcaga ttacttccgt ttccgatcac
     2341 aatgcaggga atctcccgcc cgctgcaaaa cgcaaaaatt ttacgaaagg cctccacgtc
     2401 ggccggtatg ataaagattc tggccgctcc gccaatttta aaggtggtgt gtctgctcat
     2461 cggctcattt tcaagaaccc gtgccgacag ccccgaggcg aattgtttta attcttcaaa
     2521 tttcgtaatc tccgccccct gatcgaatca tataacgccg cggacaaagc ggcgtgctta
     2581 gccctgtctg cccagcatcg cggcgccgat gatcccggcg tcattgccga gctgcgccgc
     2641 tcgaagctcg gtctgatgag cactgtagcg gctgtaacgc tctttttcaa tcattttccg
     2701 aaggggcctg atcaatacgt ccccctcatt actgacgccg ccgccgatac aaaggatctc
     2761 cggctgaaag atattgataa cgttcacaag cccgcaggca agataacgga tgtattcttc
     2821 aacaacctgt ttaccggcct catcgcccgc tttcattgcg tcaaacggcg ttcttccgtc
     2881 aacgcgggac aactcccctc cggcaatttt ccacatttta ctgccggggc tggcggtcat
     2941 ggcctcctgc gccatatgaa tcaggccggt tgccgaggaa taagcctccc agcagccctt
     3001 tctgccgcag gtacattccc ttccgtcgat ctcaatcacg gtatgtccgc actctccgcc
     3061 ggcaaaattg tggccctgca gaatgcgtcc gtcaataatg atgccgctcc ccacgcccgt
     3121 cccgagcgta atcaaaacgg ccgacttact ggatttcgcc gagccggcca taaattcacc
     3181 gtatgccgcg gcgttggcgt cgttttcgat caatacattc ttgcctattt catctttcag
     3241 catctgcgcc agcggcacat tgtcaaaggg aagattgttg gcgtattcca gcactccgcg
     3301 gacagtgtct gcggaccccg gcgcgccgat cccgacccag gcgacgtcat ccatggacac
     3361 ttccgcccga cggacgattt tccggcataa gtcagcgatc cttttcacaa tttccaccgc
     3421 tccggagcct tcccccgtcg gtgtgctttc ttttaagacc agcctcattg attcatccac
     3481 caggccggcc ttgatatttg tcccgcccaa atccacgcca agataataca agtccgtcac
     3541 gctccgttct aaatttatcg ttccggtatc gcggaaagcc gttttcgttt tcggcaaatc
     3601 cattccatta accggtcgta tttcaggttg acaatcaggt cttccggaaa atcaatctct
     3661 ttgagcattt taaaagcttc ggttacctct ccgaccgcaa agcaggaatg agcgtctgaa
     3721 ttaacaacca cgggcaccag atgctttttg cagatcaggg cgatttccct gcagtttttc
     3781 ttcgcgccct tccgggcttc aaaggaatgg ctgttcagtt ccaccagttt tccgtatttt
     3841 ttaaaaaccg gaatcgcttt ttcataatca taccggtatt tatcgctgcc gctgtggccg
     3901 atcacgtcaa caaaggggtt ttcggcaagc ttcagatagg actgcgtatg ctcatcagcc
     3961 gtcccggggg aagtcaccgg ctcgtgataa gaggcaatca cccagtcaag ctgacgcgtg
     4021 atccgctccg gaatgtcaat ctttccgtca aagccctgaa tattggcttc cgcgccatgc
     4081 agcacccgca cgccctcaat catcggcggc agggatttga gactgctgaa atgccagaga
     4141 tggcagccat cggtcagttc aggcccgtga tcggtaacgg caatggcttt cagacctttg
     4201 gccgcggcgt atcgggcaag ctcggccacc gtactgtatg cgtgggagct tgcgagggtg
     4261 tggcaatggg tgtcggcttc gaccggcacc tgacattcgg gtttaataga catcccaatc
     4321 gacttccttc gtctgttcct gcggctcggc gaggccgagc ttctcaagca gttcctgagc
     4381 ggcgttgtag cccatttttt tctgacggtt gttcatggcg gcgacttcga taataatcgc
     4441 caggttccgg cccggtttca ccggaattgt cagtgacggc acgttaatgc cgagaatctc
     4501 ggtatgttca ttttccatgc ccatccggtc ataaacctta ttgctgtccc aaacttcaag
     4561 gtgaatgatc atatccactt tttcggtgac tttcacggcc cccataccaa agatccggcg
     4621 ggcgttgaca atacccacgc cgcgcagctc gacaaaatgc cggatgtttt ccggcgaaga
     4681 gccgaccagc gttttggagc tgacccttcg gatctcgacc gcgtcgtcgg cgatcagccg
     4741 gtgaccacgc ttaaccagct cgatggctgt ctcactttta ccaacgccgc tttcacccag
     4801 cagcagaatg ccttcgccgt aaacctcaac cagaaccccg tggcgtgtaa tccttggggc
     4861 aagctcgaca ttcagatacg aaatcagccc cgacataaac ccggaagtgg gctcggatgt
     4921 gcgcagaagc ggcactttgt aggcctttgc cagttcggcc atctcatcaa acaattcgat
     4981 gccccggcag atcacaaacg ccggaggctg gtaagaaaac agcctatcaa cggcactgcg
     5041 gcgtttttcc tcgacaaatc tttttaaaaa ggaaatctcg gtcagaccaa taatctgaat
     5101 tcgactttta tcaaaataat caaagaaacc gcccagctga agcccgggcc ggtttacatc
     5161 ggcggtggaa atcatgatac gatccggatc ttccggcagg tagatgcttt caaggctgaa
     5221 ttccctgatg attttctcca gagaaacggt aaaccttcca tccatcttca tacatccttt
     5281 tcgcccaaaa tttaaggtta tatctaacta caattatatg acaaaccggt tgaggtttca
     5341 aacagttttt ctttaatcgg tgaaaattat aaaaaccact tgcttttcat tcggt
//
LOCUS       DATALY010000235         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_226501,
            whole genome shotgun sequence.
ACCESSION   DATALY010000235 DATALY010000000
VERSION     DATALY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226501"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VHR42_06595"
     CDS             <1..114
                     /locus_tag="VHR42_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3026879.1"
                     /translation="KLNKKGYHPAHVTDMEKCIGCAMCATMCPDVAIKVER"
     gene            127..1191
                     /locus_tag="VHR42_06600"
     CDS             127..1191
                     /locus_tag="VHR42_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX3026880.1"
                     /translation="MADKVLMKGNEALAEAAIQAGCHHFFGYPITPQTEVAAFMSKTM
                     PKIGGTYLQAESEIAAINMVLGAASAGVRAMTSSSSPGISLKGEGISYIAGSDLPCLI
                     INVQRGGPGLGGIQPSQADYWQATKATGHGDFQILVFAPSTVQEMVDYVFKAFDLADT
                     YRMPAMILADGMLGQMMEPVEVKKPESSAIPPKTWATNGHEGKRAHNVINSLFLKPDE
                     LEKIIVERFKRYEIIKQKEARAENYFTDDAELVLVAYGATSRIVKSAVKTAREKGLKV
                     GAVRPITLWPFPEKALKEVAKTAKAFLTVEMSMGQMVDDVRLAIDCSRPVHFFGHTGG
                     IIPTPNEVLEQIEKIMGGAK"
     gene            1191..1943
                     /locus_tag="VHR42_06605"
     CDS             1191..1943
                     /locus_tag="VHR42_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3026881.1"
                     /translation="MAVVFEKPKSLLDVPFHYCPGCTHGIVHRLVAEAIDDLGIEGRT
                     IGVAPVGCAVMSYDYFSVDMVEAPHGRAPAVATGIKRSLPDRIVFTYQGDGDLAAIGT
                     AETVHSATRGENITIIFINNAIYGMTGGQMAPTTLPGQVTQTTPYGRDVKQAGYPVRI
                     CEMLSTLDGVTYAERVSVDCVKNVLSAKKAIHKAFENQVKGLGFSIVEVVSSCPTNWG
                     LAPQEALEWVREKMIPYYPLGVYKDKTQEVGK"
     gene            1943..2479
                     /locus_tag="VHR42_06610"
     CDS             1943..2479
                     /locus_tag="VHR42_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX3026882.1"
                     /translation="MSVNHSIILAGFGGQGILFAGKVIAYAGLVDGNEVSWLPSYGPE
                     MRGGTANCSVTISDEPIGSPLIINPNVLITMNYPSYLKFIDNVTPGGKVFLDSSLIDA
                     KVERTDVDAYYVPATKLAEENSMTGRANMILLGKVLKEIGFAQYDSIIAGLKKSIPAR
                     KAAMIDANLKAVEIGMNA"
     gene            complement(2724..3260)
                     /locus_tag="VHR42_06615"
     CDS             complement(2724..3260)
                     /locus_tag="VHR42_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3026883.1"
                     /translation="MITPVLKTDRMTLRPLVLSDAEAAFRGWTSDPEVAKYMRWNLHK
                     TVGDTKEWLAAEEENIGSPTYYDWGFVFQETGELIGCGGLVRHDEHGMFELGYNIMRQ
                     YWNRGLATEAAGAITDFARRQLGVRAIVCGHAKENPASGKVMEKVGFIYQADSEYLSF
                     DGKKRFGCKEYLLTFDEQ"
BASE COUNT          845 a          867 c          912 g          855 t
ORIGIN      
        1 aaactgaata aaaaaggcta ccatcccgca catgtgacgg atatggaaaa atgcattggc
       61 tgcgccatgt gcgccacgat gtgtccggat gtggcaatta aagtagaaag gtaggtttgc
      121 gggcttatgg cagacaaagt gttaatgaag ggaaatgagg cgcttgccga ggctgcaatc
      181 caggccggct gtcatcattt tttcggatac ccgatcacgc cgcagacgga agtggcggcg
      241 tttatgtcca aaacaatgcc gaaaataggg ggaacctatc tgcaggccga gtcggaaatc
      301 gccgccatca atatggttct cggcgcggct tccgccggtg tgcgggcaat gacgtcctct
      361 tcctcgccgg gaatcagtct gaaaggtgag gggatctcct acatagcggg ctcagacctg
      421 ccctgcctga ttatcaacgt tcagcgcggc ggccccggcc tcggcggaat tcagccttca
      481 caggcggatt actggcaggc gaccaaggca accggccacg gtgattttca gattttggtg
      541 tttgctccgt cgaccgttca ggaaatggtt gattatgtct ttaaggcgtt tgatcttgcc
      601 gacacctatc ggatgccggc catgatcctg gccgacggca tgctcggtca gatgatggag
      661 ccggtggaag tgaaaaagcc ggaatcttcg gcgattcctc ccaaaacctg ggcgacgaac
      721 ggccatgagg gaaaacgtgc tcataatgtg atcaattccc ttttcctcaa acccgacgag
      781 cttgagaaaa tcattgtcga gcgttttaaa cgctacgaga tcatcaaaca gaaagaagcc
      841 cgggccgaaa actactttac cgatgacgcg gagctggttc ttgtcgccta tggcgcaacc
      901 tccagaatcg tcaagagtgc ggtcaaaaca gcacgggaaa aaggcttgaa ggtcggcgct
      961 gtccgcccga tcacgctttg gcccttcccg gaaaaagcgc tgaaagaggt cgcaaagacg
     1021 gcaaaggcat ttctgaccgt tgagatgagc atgggccaga tggttgacga tgtcaggctc
     1081 gcaattgatt gcagccgccc tgtgcatttc tttggccaca cgggtggaat tatcccgacc
     1141 cctaacgagg ttcttgaaca aattgaaaaa atcatgggag gtgcgaaata atggcggtgg
     1201 tatttgagaa accaaaatcg ctccttgacg ttccgttcca ttactgcccc ggatgcacac
     1261 acggcatcgt tcaccgcctt gttgcggaag cgattgacga tcttggcatt gagggcagaa
     1321 ccattggcgt cgcgccggtc ggctgcgccg tcatgtccta tgattatttc tccgttgata
     1381 tggtggaggc accccacggg cgcgccccgg cggtcgcgac cggaatcaag agatcactgc
     1441 ctgaccggat tgtgtttacc tatcagggcg acggcgacct tgccgccatc ggaacggcgg
     1501 aaaccgttca ttcggcgacc agaggcgaaa atataacaat tattttcatc aacaatgcca
     1561 tttacggcat gaccggcggc cagatggccc cgactaccct tccgggccag gtgacccaga
     1621 ccacacccta tgggcgtgat gtcaaacagg ccggctatcc ggtaagaatc tgcgaaatgc
     1681 tctcgacgct tgacggcgtg acctatgccg agagggtttc cgtcgactgt gttaagaatg
     1741 ttctctctgc gaaaaaagcc attcataaag cgtttgaaaa tcaggtcaag ggccttggat
     1801 tttcaattgt tgaggttgtt tcgagctgcc cgaccaactg gggcctggcg ccgcaggagg
     1861 cgctcgaatg ggttcgcgag aaaatgatcc cttattatcc gcttggagtc tataaggata
     1921 aaacgcagga ggtgggtaag taatgtctgt gaatcatagc attatacttg ccggcttcgg
     1981 cgggcagggc atcttatttg ccggtaaggt aatcgcctac gccggtctgg ttgacggcaa
     2041 cgaggtttcc tggctcccgt cttatggccc ggaaatgcgc ggcggaacag ccaactgcag
     2101 tgtgacgatt tccgacgagc ccattggttc gccgctgatc atcaatccca acgtattgat
     2161 tacgatgaat tatccgtcct atctgaaatt tatcgacaat gtgacgccgg gcggcaaggt
     2221 gtttcttgac tccagcctga tcgacgccaa ggttgagcgg acggatgtcg acgcctatta
     2281 tgttccggct acgaagcttg cggaagagaa ttcgatgacc ggtcgtgcga atatgattct
     2341 tttggggaaa gttctgaaag aaatcggctt tgcccagtat gattccatca ttgcggggct
     2401 gaaaaaatcc attcccgccc gaaaagcggc gatgatcgac gccaacctga aagccgtcga
     2461 aatcgggatg aacgcgtaac cttcgataaa tcgtttgatc aaacagattg cggcccgccg
     2521 tttggcgggc tgctttgctt ttttgcgccg ctgattgaac caataataag cagatggcgc
     2581 gagagcttct tatgcttcct gtgccggtat cgtaacgata acccggaact ttttgatgta
     2641 gtttgatcca atgaagatga aattcaagat tgtcggcgtt tgatttttga gcggtttatt
     2701 tggatagttt aatattttgg cattcattgt tcgtcaaacg ttaacagata ttccttgcag
     2761 ccaaaacgct tcttaccgtc aaaactcaaa tattcgctgt cggcctgata gataaaaccg
     2821 actttttcca tgacttttcc cgacgcggga ttttctttgg cgtggccgca tacaatcgct
     2881 cttacgccca gctgccggcg ggcaaagtct gtaattgctc ctgccgcttc ggtcgccagc
     2941 cccctgttcc agtattgacg catgatattg tagccgagct caaacatccc gtgttcgtcg
     3001 tggcggacaa gcccgccgca gccgatgagt tcaccggttt cttggaatac aaatccccag
     3061 tcgtagtagg tagggcttcc gatgttttct tcttctgccg caagccattc tttggtgtcc
     3121 ccgaccgttt tatgtaaatt ccatctcata tattttgcta cctcggggtc gctggtccaa
     3181 cccctgaagg cagcttcggc atccgataaa accagcgggc ggagggtcat tctgtccgtt
     3241 ttaagcacag gcgtaatcat aatcaaacgt cacctcacaa agaatcacaa atttttatat
     3301 tgatcctaat attataatat aaaatgcaaa atatgtaaat cattgttatt tatttgaatc
     3361 caatcataaa catgaaaaaa agcccggagt ctcctgccga taaatggcgg aaaacctccg
     3421 ggttgtgttt tacaaatgat aaatcctgtt attctaagga aaatacaggt tgcattacc
//
LOCUS       DATALY010000236         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_226866,
            whole genome shotgun sequence.
ACCESSION   DATALY010000236 DATALY010000000
VERSION     DATALY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226866"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VHR42_06620"
     CDS             complement(<1..276)
                     /locus_tag="VHR42_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026884.1"
                     /translation="MRFSIKEVFVTLIGPIGTYDNYSAAARTESKTALSCLSSFVSKK
                     SGQTGDNIDILKKLNNLDSETISSDDPLAQKFKLLKEALKPFVPVRYE"
     gene            474..1445
                     /locus_tag="VHR42_06625"
     CDS             474..1445
                     /locus_tag="VHR42_06625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3026885.1"
                     /translation="MPVDAKKVIAEILQKKGKIKDVFFAACGGSLVEFYPVKYFVENE
                     SSTIGAHLYTARELLCAKPKTLTDKTITFICSHSGNTQEAYDAAVYAKEQGSTVITFT
                     FTKDSKVNTAGDYNVLYGWGDDVPYTDGADYLALSLANELLHQVEEYPDYEKILFGLS
                     KIDGITRRAEKQVQKRAEIFAAKYYQEPILYVLGSGPSFGNAYGFSICSLMEMQWMHS
                     SYIHSAEYFHGPFEVTDKDTLYVLLENSGKTRMMDERALTFLKKYAEKYEVIDSKELG
                     IDVIDADVVDYFSPNFFYYITCVYRSELAKLREHPLSTRRYMGKVEY"
     gene            1542..2285
                     /locus_tag="VHR42_06630"
     CDS             1542..2285
                     /locus_tag="VHR42_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3026886.1"
                     /translation="MALYQDSAMPLYQQLKEDLKQKIESGRLKVGQKISSELEICSLY
                     QISRVTVRKAISELVAEGYLVKNPGKGTFVSKQKIKRKIEYLMSFSEACEANGMIPSA
                     CVTERKILQPNRDLYEGLQLEPNDQILYIQRVRYADGQPMMCENNYYPYSRFSFLMEE
                     ELNGSLYQLLEDKYGIQVAYSKNSYLEIARASNTIAKLMNVANREPLFLMYTEVCDSH
                     DRIVHVGKQYMLEDRYRFYIIDYPRVPKE"
     gene            complement(2443..2868)
                     /locus_tag="VHR42_06635"
     CDS             complement(2443..2868)
                     /locus_tag="VHR42_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026887.1"
                     /translation="MTKNEIILITIAFGVMLVFAAAAFFAYAAKKRWPVGGVLTKAET
                     SLKSSQSFVEALKPFIPVRYETIAEKVITFAQTAVAGAAQLYKIGVVEKDKRKAQATD
                     FVRNALLTEGIQITPEIEKLISLAIEGAVYALDQSCGKA"
     gene            complement(2887..3186)
                     /locus_tag="VHR42_06640"
     CDS             complement(2887..3186)
                     /locus_tag="VHR42_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026888.1"
                     /translation="MVWGAIGGSAIVSGIALALFNFFLLRKDKKDDDRAKTAQRESII
                     IMKNIQAVGHLAEATATGLKENHFDGNIDEALVYYHTARDETNEFLLQQNAIANH"
     gene            complement(3186..4334)
                     /locus_tag="VHR42_06645"
     CDS             complement(3186..4334)
                     /locus_tag="VHR42_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX3026889.1"
                     /translation="MKKKLFASLYAAAIMAAALFLPVSATAPNGAVNLRGIDISEWQT
                     VTDWASVENSVDFVYIRATLGQSYVDQKLASHVAGVKSSGKPYGFYHFFYASLDSSYV
                     RSEARNFYNAIKRYSYILTPVLDVETQSPQTRECISSNIKIFADEFYRLSGQKIMIYA
                     SPSFVNNYFDKSLSAYKYWEAHYTTASQPMNTTVWNKYDLWQYSSSGIVSGISGRVDM
                     DRATRSIYLAATSGTASACKSGDVYRVSKMPYVWNSKAKSDFSVLYSNGSRAVGHKVS
                     RGDRICILGVDYSRQLAEIVYPVKGGYMHGYIKNTESLLSNRYYNKWRNGSTSETVYN
                     AAGQITGRIYPYERATIIYKKTGLTEVLYSTAKGAETKSGFVRFGGVR"
     gene            complement(4394..>4720)
                     /locus_tag="VHR42_06650"
     CDS             complement(4394..>4720)
                     /locus_tag="VHR42_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="microcystin-dependent protein"
                     /protein_id="HEX3026890.1"
                     /translation="VFLWGNGNGSTTFSLPPNGVTYVGYEPLQVEFNAIGLTGGEKTH
                     TLTVPEMPSHTHTQQLGGALSTTQKVSGGGIIGTANSQQTGLTGGGGAHNNLQPYAVV
                     NYIIVY"
BASE COUNT         1243 a         1092 c         1093 g         1292 t
ORIGIN      
        1 ctcatagcgt accggaacaa acggcttgag ggcttctttt aacagcttaa atttttgagc
       61 caaagggtcg tcggatgata ttgtttcaga atccaaattg tttaattttt ttaaaatatc
      121 aatattatca ccagtttggc cgctcttttt tgaaacaaac gacgacagac aggaaagagc
      181 cgttttggat tcggttctgg cggcagcaga atagttatca taagtcccta ttggtccaat
      241 taacgtgaca aaaacttcct ttattgaaaa tctcaataat attatcgaca tccagcctgg
      301 ttttttaatg cctgttcgct gttttgtttc aaatggccat ctttgatgaa aaaataaata
      361 tgtcgaagaa tagcggttaa tccttgaatt ttgcggtatt atattgtatt atatcaataa
      421 tacctttcat acaatcacta aattcaaaat aacaatgatc aggaggaatt ataatgccag
      481 ttgatgctaa aaaggttatt gcagaaatcc tgcaaaaaaa aggcaaaatc aaagatgtct
      541 tcttcgcggc ttgcggaggt tccctggtag agttttatcc ggtcaaatat tttgtggaga
      601 atgagtcttc caccattggc gcgcatcttt acaccgcccg ggaattgctt tgcgcgaagc
      661 cgaaaacact gaccgacaaa accataacgt tcatctgttc ccacagcggc aacacgcagg
      721 aagcttatga cgcggccgtt tatgccaaag agcagggttc tacggtgatc acttttacct
      781 ttaccaaaga ctcaaaggtc aacacggccg gtgattacaa tgttctttac ggctggggtg
      841 acgatgttcc ttatacagat ggcgcggatt atctcgcctt gtctctggcc aacgagctgc
      901 tgcatcaggt tgaggaatat ccggattatg aaaagatcct tttcggtctg agtaaaatag
      961 acggcattac ccgccgcgcc gagaaacagg ttcagaaacg cgccgaaatt ttcgccgcga
     1021 aatattatca ggagcctatt ctttatgttc tgggcagcgg cccgtctttt ggaaacgcct
     1081 atggtttctc catctgctcg ctgatggaaa tgcagtggat gcattcgagc tacatccatt
     1141 ccgctgaata tttccacggc ccgtttgaag tgaccgataa agacaccctg tatgtgctgt
     1201 tggagaacag cggcaaaaca cgcatgatgg atgaacgcgc gcttactttc ctgaaaaaat
     1261 atgctgaaaa atatgaagtt atcgactcca aagagcttgg cattgatgtc atagacgcgg
     1321 atgtggtgga ttacttctct ccgaacttct tttactatat cacctgtgtt tacagaagtg
     1381 agcttgcgaa gcttcgggag catccgcttt cgacacgcag atatatggga aaagtggagt
     1441 attaatttaa ctcaaacggc ggcggtgcag cggggttttc cgctgcgccg ccctttttac
     1501 aggaacaaaa gctcaaatgc aggaaatcag gtgaataact catggcgctt tatcaggatt
     1561 cggcgatgcc gttatatcag cagttaaaag aagacctcaa acaaaagatc gaatcgggaa
     1621 ggctcaaggt aggacaaaag atctcctcgg agctggaaat ctgcagtctc tatcagatca
     1681 gccgggttac ggttcgaaaa gccatctcag agcttgttgc tgaggggtat ctggtaaaaa
     1741 accccggaaa gggtaccttt gtcagcaaac aaaagattaa gcgtaaaata gaatacctga
     1801 tgagtttttc agaagcctgt gaagcaaacg gaatgattcc gtccgcttgt gtgaccgaac
     1861 ggaaaatcct tcagccgaat cgagatctct atgaagggtt acagttagaa ccaaacgatc
     1921 agattttata tattcagcgg gttcgctatg cagacggcca gcccatgatg tgtgagaaca
     1981 actactaccc ctattcccgg ttctcctttt tgatggaaga agagctgaac ggttcccttt
     2041 atcagctgtt ggaagacaag tatggaattc aggtggctta ttcgaaaaac agttatttgg
     2101 agattgcccg cgcatccaac acgattgcaa aattgatgaa tgtggcaaac cgtgaacctc
     2161 tgtttttgat gtatacggag gtatgtgatt cccatgacag gattgttcac gtcggaaaac
     2221 aatatatgct tgaggatcgc tatcggttct atatcatcga ttaccccaga gttccaaaag
     2281 aatgattggg taagacggat agaaaaattt agccaaaagg gcaaaaaaaa tgaggcctta
     2341 agaaccgtta tggttcttaa ggcctctaat tatcttcaaa agtgattaca aaacgagcat
     2401 atcacacaat taatactatt ggtatcaaaa tactgctgca aattaggctt ttccacagga
     2461 ttggtccaac gcatagacgg cgccttcaat tgccaggctg atcagctttt caatctcggg
     2521 tgtaatttgg atgccttcgg tcaggagcgc atttcttaca aagtcggtcg cctgagcttt
     2581 tcttttgtct ttttccacca cgccgatttt ataaagttga gcggctcccg cgacggccgt
     2641 ctgagcaaat gtgattactt tttcggcgat ggtctcatag cgtaccggaa taaacggctt
     2701 gagggcttcc acaaaagact gagacgattt caggctcgtt tccgcttttg ttaagactcc
     2761 tccgaccggc catctctttt tggcggcata tgcgaagaat gccgctgccg cgaaaaccag
     2821 cataactccg aaagcgatgg taatcaggat tatttcattt ttggtcatct ttggttcctc
     2881 ctcaatttaa tggttggcta ttgcgttttg ctgcagtaaa aattcattgg tttcgtccct
     2941 cgccgtatga taatagacaa gggcttcgtc aatatttccg tcgaaatggt tttctttcag
     3001 gccggttgcg gtggcttccg caagatggcc gacggcctgt atgtttttca taatgatgat
     3061 gctttcccgc tgtgccgttt tcgcgcggtc atcatctttt ttgtcttttc gcaaaaggaa
     3121 aaaattaaat aatgcaagag cgattccgga aacaattgca gaaccgccga ttgcgcccca
     3181 aaccattacc ttacacctcc aaaccgtaca aagccgctct tggtttccgc tccctttgca
     3241 gtcgaatata aaacctcggt caggccggtc ttcttataaa tgatggtcgc ccgctcatac
     3301 ggataaatcc gcccggttat ctgccccgct gcgttgtaaa ccgtctcgct tgtgctgccg
     3361 ttgcgccact tattataata ccggttgctc agcagcgact cggtgttttt gatatagccg
     3421 tgcatataac cacccttgac cggataaacg atttccgcga gctgccggga gtaatctaca
     3481 ccaaggatac agatccggtc cccccggctg accttgtggc cgacagcgcg gctgccgttg
     3541 ctgtatagca cgctgaaatc tgactttgcc ttgctgttcc agacataggg cattttgctg
     3601 acccggtaga catcaccgct tttgcaggcc gaggcggttc cggatgtagc cgccagataa
     3661 atgctcctcg ttgccctgtc catatccacc cggccggaga tccctgacac aatcccactg
     3721 gaggaatact gccacagatc atatttgttc catacggtgg tgttcatcgg ctgggaggcg
     3781 gtggtgtaat gcgcttccca atacttatag gcggatagcg acttgtcaaa gtagttgttc
     3841 acgaacgacg gagaagcgta gatcatgatt ttttgacccg acaaacggta aaactcatcc
     3901 gcaaagatct tgatgttact ggagatgcat tctcttgtct gcgggctttg ggtttccaca
     3961 tccagcaccg gagttaatat gtaggaatat cttttaatgg cgttgtagaa gttccgcgcc
     4021 tcgctcctga cgtaggaaga atccagcgac gcataaaaga aatgataaaa accgtagggt
     4081 tttccggatg atttgacccc ggccacatgc gaagcaagct tctgatccac ataggattgc
     4141 cccaacgtcg cccgtatata aacaaaatcc acactgtttt ccaccgacgc ccagtcggtc
     4201 accgtctgcc actcagaaat atcaatgcca cgaagattta ccgccccgtt tggggcggta
     4261 gccgatacgg ggagaaaaag ggccgctgcc ataatggcag cggcataaag ggatgcaaat
     4321 aacttttttt tcattctgtt cctctttttg ttttttaagt gcttcccggg agggctggat
     4381 gagattactc cgatcaataa acgatgatgt aatttaccac ggcgtaaggt tggaggttat
     4441 tatgtgctcc tccacctccg gtcaagcctg tctgttgcga gtttgcagtg ccaataattc
     4501 cgccaccgga cactttctga gtcgttgaca acgcccctcc aagttgctga gtatgcgtat
     4561 gtgaaggcat ttccggaacc gtcaaggtat gtgttttttc tcctccagtt aagccgatgg
     4621 cgttgaattc aacctgcaaa ggttcgtacc cgacgtaagt aacaccgttg gggggtaaac
     4681 tgaacgttgt ggacccgttc ccgttccccc acagaaatac
//
LOCUS       DATALY010000237         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_568418,
            whole genome shotgun sequence.
ACCESSION   DATALY010000237 DATALY010000000
VERSION     DATALY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568418"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..618
                     /locus_tag="VHR42_06655"
     CDS             163..618
                     /locus_tag="VHR42_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026891.1"
                     /translation="MKALKNILIGILCGVLFVSAFYWSLVLYLLVSNSAFYPLVLILC
                     SLFANVLIGFLLSAESCRQILYKWLISLPAGIVTFLLYRQSNFVYYWLNRIWPGYGDL
                     SAGGGFAAFTYLVFFCLCFLVAVAIGVYLTKRKRHKHIPAPGAHRGGGL"
     gene            complement(624..1388)
                     /locus_tag="VHR42_06660"
     CDS             complement(624..1388)
                     /locus_tag="VHR42_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HEX3026892.1"
                     /translation="MIVAYIILGLLATLSIILLTGHGSFLIAGYNTASPEEKAKYDAK
                     KLCRAVGVTLLLTAAATAGLLLIPADSVYSTPYFIFYFIFIFADVGITLYYTNKKCLK
                     KEHKEQQEGTEAKKKKSASFIVGVCLATLPVLLLVGIALYQASQPPVFIVSNGVLNIS
                     SSFGETIRLSDIKSLQIKNSLPKSLSKTNGSDFGSILKGEFNADGRGAKVFLNAAKPP
                     FLYIEATDGLTILNDQNQTKTEALYNELKSAKMQAR"
     gene            complement(1473..1919)
                     /locus_tag="VHR42_06665"
     CDS             complement(1473..1919)
                     /locus_tag="VHR42_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026893.1"
                     /translation="MKWIKPIFIIGITAAFAVTILWFFLHGGPTDAETIRKNWKIDLT
                     GESEDVYYKDTGPSFHGDGPRITIMKYPDFYAVRKAVSWKDGPSKEVESKVSEILTSE
                     LKVEAKYRPNFKTEYKYYFYKKDWLNTICLIYKPSENCLYAVEDLY"
     regulatory      2098..2191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
BASE COUNT          525 a          521 c          488 g          675 t
ORIGIN      
        1 attctttcaa tattttcacc gcgccccata tccggctctt gatcttttgc cgatttccct
       61 tgtaatctgt tcttaaattc cttataatca aaataaaacc tttttattcc attcaattac
      121 agcgaatgga atattctcat tttacatcag ggaaggggaa gtatgaaagc tctgaaaaac
      181 atcctgatcg gcattctctg cggcgttctg tttgtttccg cgttttactg gtcgctggtg
      241 ctgtatctgc tcgtctccaa ttcggcgttt tacccccttg ttttaattct ctgctccctg
      301 tttgccaatg tactgatcgg gtttcttctt tccgcagaaa gctgtcgcca gattctttat
      361 aaatggctga tctctcttcc ggcggggatt gtgacatttc tgctctaccg acaaagtaac
      421 ttcgtctatt actggcttaa ccggatttgg cccggttacg gggacctgtc cgccggcgga
      481 ggctttgcgg cttttacata tcttgttttt ttctgcctct gcttcttggt tgctgtcgca
      541 atcggagtct atctgacaaa aagaaagcgg cataaacaca ttccggcgcc aggcgctcat
      601 cggggcggcg ggctgtaaaa tgcctaccgt gcctgcatct ttgccgattt taactcatta
      661 tagagcgcct cggtttttgt ctggttctgg tcgttgagaa tcgtcaatcc gtcggttgct
      721 tctatgtaaa ggaacggtgg tttggcagcg ttcagaaaga ccttggcgcc ccttccgtct
      781 gcgttgaatt cccctttgag aatggagcca aaatcggagc cgttggtttt gctgagactt
      841 ttcggcagac tgtttttaat ctgcagactt ttaatgtcgc tcaggcggat cgtttccccg
      901 aatgaggagg aaatgttcag gacgccgttg ctcacgatga aaaccggagg ctgacttgcc
      961 tgatacagag caatgcccac caacaggaga accggcagcg tcgccaggca gacgccaaca
     1021 ataaaggaag ccgatttttt ctttttggct tcggttcctt cttgctgttc tttatgttcc
     1081 tttttcaaac actttttgtt agtgtagtac agggtgatcc caacatcggc aaagataaaa
     1141 ataaagtaaa aaataaagta gggagtggaa tagacgctgt cggcaggaat caacagcagc
     1201 cctgccgtcg cggcggcggt aagcagcaac gttacgccga ccgcgcggca gagcttcttc
     1261 gcgtcgtatt tcgctttttc ttcgggactc gccgtgttat agcccgcaat cagaaaagaa
     1321 ccgtgacccg tcagaagaat gatggacaga gtggccagca ggcctaaaat aatatatgca
     1381 acgatcattg ttatgccttc tttattccca ataataatat taattgatgg cattatacca
     1441 cttgccgccg ggaatttcca ctgcccatat cactaatata agtcttccac cgcgtaaagg
     1501 cagttttcag acggtttgta aatcagacag atcgtattca gccaatcctt cttatagaaa
     1561 taatacttat actccgtttt aaaattcggc ctatatttag cttcgacttt taattcagaa
     1621 gttaaaattt cggaaacttt ggattcaacc tctttgctgg ggccatcctt ccagcttacg
     1681 gcttttctga cggcatagaa gtccggatat ttcatgatgg tgatcctcgg cccgtcccca
     1741 tggaaggaag gtccggtgtc tttgtaataa acatcttcgc tttccccagt caaatcaatt
     1801 ttccagtttt tccggattgt ttccgcgtcc gtgggacctc catgcagaaa aaaccacaaa
     1861 atcgttacgg cgaaagcggc tgtgattccg attataaata tcggctttat ccatttcatt
     1921 tggcgctgcc tcctaaagca aaatccggtt atgcatggtg agaacaacat tccactttta
     1981 gtttaccata aattttattt tcttcaatga gagttttgct gctatcgatt ctacaccttg
     2041 acaaaccggc ctgcatatat tactatataa atatctcttt gcactgcata taaatctaca
     2101 gtacaaactg agcaggcgat gaaccgatag gtttgtgggg ctgggccggc gacggcaacg
     2161 gatgaatcta tgtcacatct gtgggacagt tgtatgttcc atgggtgtg
//
LOCUS       DATALY010000238         5151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_484075,
            whole genome shotgun sequence.
ACCESSION   DATALY010000238 DATALY010000000
VERSION     DATALY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484075"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VHR42_06670"
     CDS             <1..465
                     /locus_tag="VHR42_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX3026894.1"
                     /translation="NFVEIRVQSGTETEVSEIGSLFEISAYETSASDFFGKILQNHAE
                     LVLDVRLKNESQLCGFTKKKDLAYFVPAICGAVYIHDCFFTPEPEFLDRYLHGWIHWE
                     EYAQRYRASMEARNAAGYFQEHYGSYSGVCLIGTGTRKRHSHSEVLRELLQG"
     gene            488..1042
                     /locus_tag="VHR42_06675"
     CDS             488..1042
                     /locus_tag="VHR42_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX3026895.1"
                     /translation="MDTFASTYGAGTGQETIFDKLKEEHEHILAFCDYVRNTCEVCVE
                     KHAVPVAEFRSMIQFIRDYADGHHHRKEEKILFKEMVDRLGGIAVNLVQHGMLVEHDL
                     ARLLVKDLEKALEEYEQTSSGRSLLNVIANAVGYCNLLERHIEKENTVVYPYAEKHLE
                     PDVIRSMTEQSEKMNREDFFMEQV"
     gene            1077..1775
                     /locus_tag="VHR42_06680"
     CDS             1077..1775
                     /locus_tag="VHR42_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HEX3026896.1"
                     /translation="MKYQAAENCYAIGKSIRKPEIEISFLSYLITGSANILIDTLPDR
                     CESMLKEEIASILGEKPIDAIILNHSEEDHSGALPGILRQYPGTPVYCSQACRKRLSE
                     KLPEADFRPVKTGETVTFGSNAFTFYETPGLHWDDNMVTYWNDNDVLFSNDLFGQYAA
                     AEPPIDRNYSVGALLAAADRYYEKVFSSAPQSEKNVVLKLAELPIRRIAPGHGLILED
                     EISAILQLYRNKIE"
     gene            complement(1958..2146)
                     /locus_tag="VHR42_06685"
     CDS             complement(1958..2146)
                     /locus_tag="VHR42_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX3026897.1"
                     /translation="MKAKLDRSGCISCGLCAQTCPEVFRIADDGVAEVFLENVPEALE
                     NSAVEAQNGCPVSVITVE"
     gene            2357..2653
                     /locus_tag="VHR42_06690"
     CDS             2357..2653
                     /locus_tag="VHR42_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HEX3026898.1"
                     /translation="MPEQNNDSSKLPMQTLKDIPQPNADAKQITGLVKEGGRITGYQL
                     SDGEVLSKQEGVQLAKQGGIRGVGVATRNGNEYLKSLPDDTEGNNLSNLPSVSQ"
     gene            complement(2715..3185)
                     /locus_tag="VHR42_06695"
     CDS             complement(2715..3185)
                     /locus_tag="VHR42_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1453 domain-containing protein"
                     /protein_id="HEX3026899.1"
                     /translation="MGSSLSYLTVIVIVMKLSKISSGKSKRVKFPNMWLIPILFIAMI
                     FQDCSKASPVRFLGAVIVAAFFFVGAFIGVCRGKTLHYYQDERDREVYYQESYRSLVL
                     YILLIAAKWLVQQISAGPLASIISLSMLFFACGSIIGRCLFITAKYLSYHRSVS"
     gene            complement(3210..3695)
                     /locus_tag="VHR42_06700"
     CDS             complement(3210..3695)
                     /locus_tag="VHR42_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026900.1"
                     /translation="MNQTMIGIVISVAVIAFLIWNHFKPQNIDFRIVLVPSILAVLFV
                     GFAFQYPTLKGDAAGLALRFLLGLAIGVLQGCLAKLVSEDGGIVSTGTKTGLACWLIF
                     VPVRLLILPWFSVIAPGVHNLENSSLAGIAALCIFTGFFLSKGTTLLLRKARLLTAGV
                     G"
     gene            complement(3792..4445)
                     /locus_tag="VHR42_06705"
     CDS             complement(3792..4445)
                     /locus_tag="VHR42_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3026901.1"
                     /translation="MQVVIADDQPIIRQGLKMILELDENLHFAGEAGTGVELMEFLNR
                     TPCDVVLMDIRMPQMDGMEATLRVKNRFPKTKVLILTTFEDDEYIFHCLKNGADGYLL
                     KDADPDEIIRAIYTVNSGNILLHPKITTKVVSALNAGAPITAAAGEEPPELLLLTARE
                     REVAELVAQGKSNRDIAAVLFVAEGTVKNHLTKVLDKLSLKSRNELIVYMTKVTGGA"
     gene            complement(4479..>5151)
                     /locus_tag="VHR42_06710"
     CDS             complement(4479..>5151)
                     /locus_tag="VHR42_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX3026902.1"
                     /translation="ALNSKNQQLLHYQDQLKELALSAERNRVAQELHDSLGHTLMAIH
                     MNIKVLEKLGHSNPEKETRIISNLDEIVQNGINQLRETVFQLKNESAKKPLKSSLTEM
                     IGRLSENGGTRIELECDDRVDECLPEIKDAVYKAVKEGITNSVRHGKASEIRVSVSVR
                     EEQIHMKISDNGCGCDEIKKSYGLQGIEERFAAWDGHISYQSKKGQGFVITADVPIFE
                     HKSEK"
BASE COUNT         1358 a         1320 c         1224 g         1249 t
ORIGIN      
        1 aatttcgtcg aaattcgggt acaatcaggt acagaaacgg aggtgagtga aatcggcagt
       61 ctgtttgaaa tcagtgctta tgagacgtcg gcctccgatt tcttcggtaa aatccttcag
      121 aatcacgccg agctagtgct ggatgtgcgg ctcaaaaacg agtcccaact ttgcggcttt
      181 acaaagaaaa aagacctcgc ctattttgtc cccgcaatct gtggagccgt ttatatccac
      241 gactgctttt ttacgccgga gccggagttc ctggaccgct atctccacgg ttggattcac
      301 tgggaagaat acgcgcagcg ataccgggct tccatggaag cccgaaacgc tgccggttat
      361 tttcaagagc attacggcag ctattccggt gtctgtctga tcggcacggg cacccggaag
      421 cggcattctc acagcgaagt ccttcgagaa cttttacagg gctgattgac acagggaggg
      481 gaaaaaattg gatactttcg cttcgacata cggggctggg acagggcagg aaacgatttt
      541 tgacaaatta aaggaggagc acgaacacat tctggccttc tgcgattatg tgagaaacac
      601 ctgtgaggtc tgtgtggaaa aacatgccgt gccggttgcg gagttccgca gtatgattca
      661 atttatccga gactacgcgg acggccacca tcaccggaag gaagagaaaa ttttattcaa
      721 agaaatggtc gaccgtttgg gaggcatcgc cgtaaatctg gttcagcacg gcatgctggt
      781 agagcacgac ctagcgcgcc ttctggtaaa ggatctggaa aaggcgctgg aggaatacga
      841 acagacttcg tccggcagga gcctgctcaa tgtaattgca aatgccgtcg gttattgtaa
      901 tctgctggaa cgccacattg aaaaagaaaa cacggtcgtc tatccttacg cggaaaaaca
      961 tctcgagccg gatgtgatcc ggagcatgac ggagcagtcg gaaaagatga accgtgaaga
     1021 cttttttatg gaacaagttt aaagttctat ttataatttt ataaggaggc aaaaccatga
     1081 aatatcaggc agcggaaaac tgctatgcaa tcgggaaaag cattcggaaa ccggaaatcg
     1141 aaatctcctt tctgtcttat ctcatcacag gcagcgcaaa cattttaatc gacaccctcc
     1201 ccgaccggtg cgaatccatg ctgaaggagg aaattgcttc gatccttggg gaaaaaccga
     1261 ttgacgcaat tatcctgaat cattccgagg aagaccacag cggagcctta ccggggattc
     1321 tgcggcaata cccgggaact cccgtgtact gctcgcaggc ttgccggaaa cgtctttccg
     1381 aaaagctgcc cgaagccgat ttccgaccgg tcaaaaccgg ggaaaccgtg acattcggaa
     1441 gcaacgcatt cacattttat gagactccgg gactgcactg ggacgacaac atggttactt
     1501 attggaatga taacgatgtc ttattctcca acgacctatt tgggcagtat gcggcggcag
     1561 agccgccgat agaccgcaat tattctgtcg gcgcacttct tgccgcggcg gaccgctact
     1621 atgagaaggt attttcctcc gcgccgcagt ccgaaaaaaa tgtggttttg aaactggcgg
     1681 agcttcccat ccgcagaatt gctccgggac acgggctgat tctggaggac gagatttccg
     1741 caatcctaca gctttatcgg aataaaatcg aatagccagc gaaaacaaaa cggccgccca
     1801 ctgcaggaga atcgtctcca aagcgaaggg cggctgtttc tgtttcgaac ctgaggaccg
     1861 gaactccgtc cggtatttgt ttcatggttt aaaaagcagc gtcggtccgc ttttcagcgg
     1921 gaccgacgct gctgcaggca tggaatgagg agttgcttca ctcaaccgtg atcaccgata
     1981 ccgggcatcc gttctgcgct tccaccgcac tgttctcaag agcctccgga acattctcaa
     2041 gaaagacttc cgccaccccg tcgtcggcga tccgaaaaac ctccggacag gtttgcgcac
     2101 acaggccgca tgaaatgcag ccgcttctgt caagcttcgc tttcataacg ttacttcctt
     2161 tcacaattgg atttctcgaa tttatagtat cagcttttaa agcatatttc cgttgtattt
     2221 gcaacagaaa tatgaaattt atgatgatga tcaagacaaa ctgccaaatg agtgggaatc
     2281 atctttgttt gccgcttcct ccggcataag atttgatctc cttggaaaac tatttaaaaa
     2341 aggaggctgg tttaccatgc cggaacaaaa taacgattca tcaaagctgc ccatgcagac
     2401 gctgaaagat attcctcagc ccaacgcgga tgccaaacag attacgggat tggtgaaaga
     2461 aggagggcgc atcaccgggt atcaactgtc ggacggagag gtgctcagta agcaggaagg
     2521 cgttcagctt gccaagcagg gcggaatccg cggagtcggc gtcgcgacaa ggaacggaaa
     2581 cgagtatctg aaatctttgc cggatgacac cgaaggcaac aatttgagca atttgccttc
     2641 ggtctcgcag taaaacagaa cgaggcactt tgcgacgttt tgcaaagtgc ctcgttttta
     2701 gtttcaagga caaatcaact cacgcttcta tggtacgaga gatattttgc ggtaataaaa
     2761 aggcatcttc ctatgatgga gccgcacgcg aaaaagagca tagaaaggct gattatcgac
     2821 gccagcggcc ccgcgctgat ctgctgaacg agccatttcg cagcaatcag caaaatgtac
     2881 agaacaaggc tccgatagct ctcctgataa tagacttccc gatcccgttc atcctgatag
     2941 tagtgcagcg ttttcccccg acagacaccg ataaaagcgc caacaaagaa aaaagctgca
     3001 actattacag caccgaggaa acgaaccgga gaagccttcg aacaatcctg aaaaatcatg
     3061 gcaataaaca ggatcgggat aagccacatg ttcgggaatt taacgcgctt acttttcccg
     3121 gaggaaatct ttgaaagctt cataacaatc acaattaccg tgagatagga cagcgaagaa
     3181 cccataattt ctccttttcc ggcagcggct tatccgaccc cggcggtcag caatctcgct
     3241 tttcggagca aaagggtggt tccctttgac agaaagaaac ctgtaaagat acaaagggcc
     3301 gctatgccag caagcgacga attttccagg ttatgaactc cgggcgcgat cacgctgaac
     3361 cacggaagaa tcaacagcct caccggcaca aaaatcagcc agcatgccaa gcctgtcttg
     3421 gtaccggtgg aaacgatgcc gccatcctcc gaaacgagtt ttgcgaggca cccctgtaaa
     3481 actccgattg cgagtcctaa cagaaaacgc agggccagcc cggcagcgtc acctttgagc
     3541 gtgggatact gaaacgcaaa tcccacgaac agcaccgcaa gaatgctcgg cacaagaact
     3601 attctgaaat caatgttctg gggtttaaaa tggttccaaa ttaaaaaagc gataacggca
     3661 acggaaataa caatcccaat cattgtctgg ttcacttggt tttcctcact ttcctgagcc
     3721 agtataccat ttccgttccg aataaacatc taccgaaggt catgagtcgg gtcatgaaaa
     3781 aagcggggct tttatgcccc gcctgtgacc ttcgtcatat acacaatcaa ttcatttcga
     3841 ctctttaaac ttagtttatc cagcactttg gtcagatggt tctttacggt tccctcagca
     3901 acaaaaagta ccgcggcgat atctcgattg cttttcccct gagcgactag ttccgcgact
     3961 tccctttccc ttgcagtaag gagcaaaagc tccggcggct cttcgccggc agccgcggta
     4021 atgggcgccc ccgcattcaa ggccgacacc actttcgttg ttattttagg gtgcagaaga
     4081 atgttgccgc tgttcaccgt ataaatggcc cgtatgattt catccgggtc cgcgtccttt
     4141 aaaagatagc cgtccgcgcc gtttttcagg cagtgaaaaa tatattcatc gtcctcaaag
     4201 gtagtcaaaa tcaacacttt tgttttcgga aatctgtttt tcacccgcag ggtcgcttcc
     4261 ataccgtcca tttggggcat gcggatatcc atgaggacga cgtcgcacgg cgttcggttc
     4321 agaaattcca tcagttccac gccggtgcct gcttctccgg caaaatgaag attctcatcc
     4381 agttccagaa tcattttcag tccctgccgg atgatcggct gatcatccgc aatgactacc
     4441 tgtatcataa caaaccgaac ctttcccact gctttttatc atttttccga tttatgctca
     4501 aaaatcggta catccgccgt gataacaaag ccctgaccct ttttgctttg gtaggaaatg
     4561 tggccgtccc atgcggcaaa ccgttcttcg attccctgca ggccataaga ctttttgatt
     4621 tcatcgcatc cgcagccgtt atccgaaatt ttcatatgaa tttgctcctc gcgaacggaa
     4681 accgaaaccc tgatttcact cgcttttcca tgtcggaccg agttggtaat gccttccttc
     4741 acagctttat agacggcgtc cttgatttcc ggcaggcatt cgtcgacccg gtcatcacat
     4801 tccagctcga ttcgggttcc cccgttttcc gaaagccgac caatcatttc cgtcagcgat
     4861 gactttaacg gcttcttcgc actttcattt tttaattgaa atacagtttc gcgaagctga
     4921 ttgataccgt tctgaacaat ttcatcgagg ttgctgatga tccgggtttc cttttccgga
     4981 ttactgtgcc ccagcttctc cagcacctta atattcatgt gaattgccat cagggtgtgg
     5041 ccaagggaat cgtgcagctc ctgcgccaca cgattccgct ccgcgctaag ggcaagctct
     5101 ttcagctgat cctgatagtg cagcagctgc tgatttttac tgttcagcgc g
//
LOCUS       DATALY010000239         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_484180,
            whole genome shotgun sequence.
ACCESSION   DATALY010000239 DATALY010000000
VERSION     DATALY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484180"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VHR42_06715"
     CDS             complement(<1..505)
                     /locus_tag="VHR42_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3026903.1"
                     /translation="MAFINTNFLDLKQSYLFSEMGKRVAAFQAGNPGRKVISLGIGDV
                     TRPLPEACLSAMHRAVDEMGDSSTFRGYSPDQGYAFLREAIVRGDFQQRGVDLSPDEI
                     FVSDGAKSDTGNIGDIFNTINTVAITDPVYPVYADTNIMAGREIKIISCTEETGFAPL
                     PPDFKADI"
     gene            complement(565..1398)
                     /gene="dapF"
                     /locus_tag="VHR42_06720"
     CDS             complement(565..1398)
                     /gene="dapF"
                     /locus_tag="VHR42_06720"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX3026904.1"
                     /translation="MHFYKMQGLGNDYVYVNCFENEVENPSELSIRVSDRHFGIGSDG
                     LILVCPSSVADVKMKMYNADGGEGRMCGNGIRCVGKLAYEKEICRNRSLTVETLSGIK
                     FLKLFENDGVVEKVQVDMGDPALEIEKIPALADERRIVDYPIEVDGKEYRITCVSMGN
                     PHAVIFTKNIDDLDLEKIGPAFEHHPMFPESVNTEFVEVIDERNLKMRVWERGSGETL
                     ACGTGACATVVAACINKITPRNEDVTVHLRGGDLNVKWDNDRVWMTGPAEFVFEGDIE
                     I"
     gene            complement(1511..2068)
                     /locus_tag="VHR42_06725"
     CDS             complement(1511..2068)
                     /locus_tag="VHR42_06725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3026905.1"
                     /translation="MKLEEKTIEARYIYKGSIINLRKDRVELPTGREASREVVEHNGG
                     VGVAAVDEDGNVYLVNQFRYPYQAEMLEIPAGKLVKGEEPLACGKRELKEETGICAKE
                     YASLGQLYPSPGYTDEIIYLYFARGLEYGEQQPDEDEFLDVVKIPLEKAVDLVLSGEI
                     KDSKTQTALLKVWMILSGCAPSEKA"
     gene            2184..>2534
                     /locus_tag="VHR42_06730"
     CDS             2184..>2534
                     /locus_tag="VHR42_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX3026906.1"
                     /translation="MDRFFDETRPMGYDELYCVTQKLKKEYDFLQSFSIGKSVLGRSI
                     ITYLLGKGRPCILYVGGHHALEYITSMLLVRLTEDLCRCYRSGENLGGFDIHSILSAC
                     SVYIIPMLNPDGVEI"
BASE COUNT          606 a          718 c          557 g          653 t
ORIGIN      
        1 taatatccgc tttaaaatcc ggaggaagcg gcgcaaaacc agtctcttcg gtgcaggaaa
       61 tgatcttgat ttctcttccc gccatgatgt tggtatcggc gtaaaccgga taaacaggat
      121 ccgtaatcgc aacggtgttg atcgtattaa aaatatcccc gatattaccc gtgtcacttt
      181 tggctccgtc gctcacaaat atttcgtccg gcgaaaggtc gacgcccctc tgctgaaaat
      241 cccctctgac aatcgcttcc cggagaaacg catatccctg atccgggctg tatccccgga
      301 atgtggagga atcgcccatt tcatccaccg cccgatgcat cgccgaaagg cacgcctccg
      361 gcagcggacg ggttacgtcg ccgattccga gactgatcac tttccggccg ggattccccg
      421 cctgaaaagc cgctacccgc ttgcccatct cagaaaacag gtagctttgc tttaaatcca
      481 gaaaatttgt attaataaat gccactgata ttcctccctt ttatctttgc ggtcatccca
      541 gccctgtcgg caaaaaagcc gcttttagat ttcgatatcg ccttcaaaca caaattcagc
      601 ggggccggtc atccagaccc tgtcgttatc ccatttgaca ttcaggtccc cgccccggag
      661 atgaaccgta acatcctcat tgcggggcgt aatcttgttg atgcaggcgg ccaccactgt
      721 ggcgcaggcg cccgttccgc aggcaagcgt ctcgccgctg cctctttccc aaacccgcat
      781 cttcagattt ctctcatcga tcacttcgac aaactccgtg ttgacgctct ccggaaacat
      841 cggatgatgc tcaaaagccg gaccgatttt ttcaagatcg agatcgtcga tattttttgt
      901 aaaaatcacg gcatggggat ttcccattga aacgcaggtg atccggtatt cttttccgtc
      961 gacttcaatc gggtagtcaa ctatcctgcg ttcatccgca agggccggaa ttttttcgat
     1021 ttccagcgcg gggtctccca tatccacctg aaccttttca acgactccat cgttttcaaa
     1081 cagtttgaga aactttattc ccgacagcgt ttccaccgtc agcgaccggt tgcggcagat
     1141 ttccttttca taagccagtt ttccgacaca gcggatgccg ttgccgcaca ttctgccttc
     1201 gccgccgtca gcattataca ttttcatttt aacatccgca acgctggacg ggcaaaccaa
     1261 aatcagcccg tcggagccaa ttccgaaatg acggtcggaa acgcgaatag aaagttccga
     1321 cggattctcc acttcattct caaaacagtt gacataaaca taatcgttgc cgagaccctg
     1381 catcttataa aagtgcaata acataacatt cctgcctttc cataaaacag tatttgcgca
     1441 aacggccatt ttctgaagaa cgactggtat ttaaccaagc cgtgagaaca tataataatc
     1501 cggtaagata ctaagccttt tctgaaggcg cgcagccgga caaaatcatc catactttaa
     1561 gcagcgccgt ctgggttttt gaatctttga tttcacccga gagcaccaga tccacggcct
     1621 tttcaagcgg tatcttcact acgtcaagga actcatcctc atccggctgc tgttcgccgt
     1681 actcgaggcc gcgtgcaaaa taaagataaa taatctcatc ggtatagccc ggggacggat
     1741 aaagctgccc cagacttgca tattccttcg cacaaatgcc ggtttcctct ttgagttcgc
     1801 gttttccgca ggcaagaggt tcctcccctt ttacaagctt acccgccgga atttcaagca
     1861 tctcggcctg atagggatag cggaactggt tgacaagata aacgttaccg tcttcatcaa
     1921 cagccgcgac gcccacgccg ccgttgtgtt ccacaacctc ccttgaagct tccctgccag
     1981 tcggcagttc cacccggtct ttgcgcaaat taataatgct tcctttgtaa atatatcggg
     2041 cttcaatcgt tttttcttcg agtttcaatg atttcttcct ttcctgccct cttggagccg
     2101 gattcatccg gcggaacccg gacacccccg ggaggagagc gcccgatcaa attgccgccc
     2161 gccgcggcag actggaggta taaatggacc gatttttcga tgaaacaaga cccatgggtt
     2221 atgacgagct ttattgtgta acgcaaaagc tgaagaaaga gtacgacttt ctccagagtt
     2281 tttcaatcgg gaaaagtgtc ctcggccgga gtattattac ttatctgctt ggaaaaggcc
     2341 gcccctgtat tttatatgtg ggaggacacc atgcgctgga atacatcaca tccatgctgc
     2401 tggtacgtct gacggaggat ctctgccgct gttaccgttc gggggaaaat ctgggcggct
     2461 tcgacattca cagcatcctg tcggcctgct cggtttacat catccctatg ctcaaccccg
     2521 acggagtgga aatt
//
LOCUS       DATALY010000240         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_139351,
            whole genome shotgun sequence.
ACCESSION   DATALY010000240 DATALY010000000
VERSION     DATALY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139351"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..526)
                     /locus_tag="VHR42_06735"
                     /pseudo
     CDS             complement(53..526)
                     /locus_tag="VHR42_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            811..1683
                     /gene="galU"
                     /locus_tag="VHR42_06740"
     CDS             811..1683
                     /gene="galU"
                     /locus_tag="VHR42_06740"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX3026907.1"
                     /translation="MKIKKAIIPAAGLGTRVLPASKAVPKEMLPIVDKPAIQYIVEEA
                     AKAGIEDILIITNRGKSIMEDHFDRAPELEKRLEDSGKTQMLQDVIDVTKLANITYMR
                     QKETKGLGHAVLCARSFVGNEPFAVLYGDDVIMSQKPVVAQLCEAYEEYSLGVVGVKE
                     VPAEDIHKYSSLAVTPLKDRVFRVTDMIEKPSSPEKVLSLYSILGRCVLPPEIFDILE
                     TTPPGAGGEIQLTDAMKELTRSKSMIAVDFEGTRYDMGNKLGIMKAAVETALTHSEIG
                     RDFRSYLKELAKTL"
     gene            1839..2135
                     /gene="rpsF"
                     /locus_tag="VHR42_06745"
     CDS             1839..2135
                     /gene="rpsF"
                     /locus_tag="VHR42_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX3026908.1"
                     /translation="MANVTGKYESVIILKANLGDEATAALVERFKSLVSSNGTLDSID
                     EWGKRRLAYPISDEMDGYYFLMTFTADKDFPAEFDRQLKITDGVLRSLIISKDK"
     gene            2152..2598
                     /locus_tag="VHR42_06750"
     CDS             2152..2598
                     /locus_tag="VHR42_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3026909.1"
                     /translation="MLNVAVLMGRLTADPELRHTSSDIPVTSFTMAVDRGYVKAGAER
                     QTDFIDIVCWRGTAEFVSRYFHKGMMVAVEGSIQTRSYTDSQNIKRKAFEIVANNVHF
                     AEPRRDSNPSQAAPSYDHSQNNQAAAISSGDNGDFVEIDGDDDLPF"
     gene            2638..2898
                     /gene="rpsR"
                     /locus_tag="VHR42_06755"
     CDS             2638..2898
                     /gene="rpsR"
                     /locus_tag="VHR42_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX3026910.1"
                     /translation="MDRDRGERGDRNDRRGRKGRRKVCSFCVDKVETIDYKDIAKLRR
                     FLSERAKILPRRVTGTCAHHQRELTIAIKRARHIALLPYVSD"
BASE COUNT          770 a          745 c          704 g          699 t
ORIGIN      
        1 cagtttcagc tgccggatat cgctgccaaa aaatttaaga aggataaaat cgccgatcag
       61 cccagtgggt atacatccgg tatagcatct cggcccaatc ctgggcctcg gttcctccgg
      121 ctccggcatg gaagttcagg atggcattcc ttgcgtcgta ttcgcccgtc agaagcgtag
      181 caagcttcat cgtgctgagg ccggtttcaa actttttgat cagttcacag atttcgtcaa
      241 agaccgattc atcccccgcc tcgtcggcca gctcgaccat ggccatcgcg tcgtcaaatt
      301 caccggataa acttttgtat gcggcaattt tatctttaag ctgccttgtt ctgcgcagaa
      361 ctttctgaga attttccatg tcatcccaga agccctgatg cgaagcttcg gcctccagct
      421 ttgcaacctc ctgctccgcg ctctccagcc caagcgcctc gcccagatcc ctgagctcgg
      481 gccttcgccc ctcaagcttt aattttaatt cttcaaactc aagcaataca acccatcctt
      541 cattctgtta gttataatta aagaattccc cctaccggac accgtcgaac cggctccgga
      601 aagggaaaca gaacagccct tgaaaaaacc tgtatgtaaa ctttattata aatgtttttc
      661 ggacgattgt aaagaaaagc ggcacggaag aaattcatgc gggtgttccc ccccgctttt
      721 cgcatttttt gtgaataact gcccttaagt gtttactatt ttcaattaaa atgctaaaat
      781 attggataac atatattggg aggaatctcc gtgaaaatca aaaaagcaat cataccggca
      841 gccggactcg gaacccgcgt actgcctgca tcgaaagcgg ttcccaaaga aatgcttccg
      901 attgtggaca agcctgccat tcaatatatt gtcgaggaag cggcaaaggc aggtattgaa
      961 gatattttaa tcatcaccaa tcggggaaaa tcgatcatgg aggatcattt cgaccgcgct
     1021 ccggagcttg aaaaaaggct ggaggattcg ggaaagacgc agatgctcca ggacgttatc
     1081 gacgtaacaa agctcgcgaa catcacctat atgcgccaaa aagaaaccaa aggcctcggc
     1141 cacgccgtac tctgcgccag atcgtttgtc ggcaacgaac cgtttgccgt tttatacggc
     1201 gacgacgtca ttatgagtca aaagccggtg gtcgcccagc tttgcgaagc ctatgaagag
     1261 tattctctcg gagttgtcgg cgtaaaagaa gtcccggcgg aggatatcca caaatactcc
     1321 tctctggctg ttacccctct gaaagaccgg gtgttccggg taaccgacat gattgaaaag
     1381 ccctcctcgc ccgagaaggt cctttcgctc tactcgattt taggacgctg cgttctgccc
     1441 ccggaaatct tcgacattct tgaaaccacg cctcccggag cgggcggaga aattcagctg
     1501 accgacgcga tgaaagagct gacccgctca aaatccatga tcgccgtgga ttttgagggc
     1561 acacgctacg acatgggcaa taaactcggc attatgaaag ccgcagtgga aacggctctc
     1621 acgcattccg aaatcgggcg ggatttcaga agctacctca aagagcttgc gaaaactctt
     1681 tagtcgggag cctgcaaaac ttgacggtaa cgccgatgtg tggtaaaata ctactgtttt
     1741 actgctgaca gaagcctcgg gttttatgcc gggccacggc agcaggatac atccttaccg
     1801 ctacggcggt catatgtcca gaaggaggtg ctttataaat ggcaaacgta acaggcaaat
     1861 atgaatcggt catcatactc aaagcaaatc tcggcgacga ggcaacagcc gcgctggttg
     1921 agagattcaa gagtcttgtg tcttccaacg gaacgctgga ttcgattgac gagtggggaa
     1981 aacgccggct cgcctacccg atcagcgacg aaatggacgg ctactatttc ctgatgacct
     2041 tcacagccga caaagatttt ccggctgaat tcgaccgtca gcttaaaatt accgacggcg
     2101 tacttcgctc cttaattata tctaaggaca aataaagggc gggtgacttt catgcttaat
     2161 gttgcagttt taatgggcag gcttaccgcc gaccctgagt tgcgtcatac cagctcggac
     2221 attccggtga cttcatttac aatggcggtt gaccggggct atgttaaagc gggtgccgaa
     2281 cgccagaccg attttattga tatcgtctgc tggcggggga cggctgaatt cgtttccaga
     2341 tattttcata aaggtatgat ggtggctgtc gaaggttcta ttcaaacacg atcctacaca
     2401 gacagtcaga atattaagcg taaggctttt gaaattgtcg ccaacaatgt tcactttgcc
     2461 gagccgcgca gagacagtaa tccgtctcag gcagctccat cctatgacca cagccagaac
     2521 aatcaggctg ccgccatttc gtcaggcgac aatggtgatt ttgtggaaat cgacggtgac
     2581 gacgacctac cgttttaaca cggcaggttc tactatgttt aaaaggaggg tattttaatg
     2641 gatagagata gaggcgagag aggcgacagg aacgacagaa gaggcagaaa aggccgcaga
     2701 aaagtctgtt ctttttgtgt tgacaaggtt gagaccattg attataaaga catcgcaaag
     2761 ctccgcagat tcctttccga gcgggcgaag attcttcccc gtcgtgtgac aggcacctgc
     2821 gcccatcatc agcgggaact taccattgct atcaagcgcg cacggcatat cgccctgctg
     2881 ccttatgtga gcgactaaaa tgaaatctta aaaccccc
//
LOCUS       DATALY010000241         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_397775,
            whole genome shotgun sequence.
ACCESSION   DATALY010000241 DATALY010000000
VERSION     DATALY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397775"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            877..1437
                     /locus_tag="VHR42_06760"
     CDS             877..1437
                     /locus_tag="VHR42_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HEX3026911.1"
                     /translation="MNPCRLPTQVAVMANSLYHSLQGQYFVGYADNMFFEKDKNAWAA
                     LVNPCDSGVNLFVNVWTVTDLYEPPIRTQIWFNASLPGDPIQSRLVTPSNTALRPLPE
                     PKIRLMQASNVVGTPEGGVKGFVRRTLPGETVVAEEDGKFIIPPGGNFAVFLSNPEGT
                     NTPASVRVAYGWWEEKICGHQYKLAV"
BASE COUNT          420 a          336 c          342 g          428 t
ORIGIN      
        1 cagcaaattt cccagctctc gccggttttt tccctgccgg ttattcgtcc gtaaagagaa
       61 tggagcttcg ttcctcccca caatttaggt ttgtaaaccg gcgtaaactt taacggatat
      121 aacacagcgc catcaacctc caatgttttc catttctatt tgagatataa aaaggctctt
      181 tccaatatcg aatgatatta aaaagagaga caaacctttt attctttatc tttcttttac
      241 atcatattta gcactctaat acggggagtg ctaatgcttt taatctaatg ctattcctga
      301 agtctgtcaa ttccattatc agccaatatt gagcttttac tcctcatatc cttgaattaa
      361 gtccttatct cttcgatttt ctttcgattt cgtcgcatta attttttgca gttatgaatc
      421 aggtttattg caactgcatg atgaggaata ccaaataagc cgttcgggat attgtttctg
      481 ttagaatgca gtcatgtttg aaccgttcca gacaggcgca tatcacattt tatttttacc
      541 cggaataaaa tatacaggga ttcagaagat ttatatctgc aagagaatgc ggcggcaagt
      601 attcgggcaa aatcggacta ccctgccatt caggccgcgg ctgatgcagc ggctccccag
      661 gatataacat tgatcaaaag cggcacataa aatcacgaat ataaaaccag taatgctccc
      721 ggtttgtatg atttatttac cggagcagaa aaagcagaat ttaagaggtg caataatgaa
      781 catggataaa ttgtttccgt atgatctggc tgttcaaaaa aataaatgga aagagctgag
      841 cgcagcgcgg gctgtcgccg ggaaaggagt cgaatcatga atccatgcag gctgccgaca
      901 caggtagcgg ttatggccaa ttcactttat cattccctgc agggccaata ttttgtgggt
      961 tacgccgaca acatgttttt tgaaaaggat aaaaacgcat gggcggcgct ggtcaaccca
     1021 tgtgattcgg gggtaaacct ctttgtgaat gtttggacgg tgacggacct ttatgagccc
     1081 ccgatccgga cgcaaatctg gtttaatgcc tcactgcccg gcgatccgat ccaatcaagg
     1141 ctggttactc cttccaacac ggccctacgc ccgttaccgg aaccaaaaat ccggctcatg
     1201 caggcttcca acgtagtcgg cactccggag ggcggcgtca aggggtttgt cagaagaacg
     1261 cttccgggtg aaaccgtcgt ggctgaagaa gatggaaaat ttattattcc tcccggaggg
     1321 aattttgccg tttttctttc caatcccgaa ggaaccaata cgcccgccag cgtgagagtt
     1381 gcctacggtt ggtgggagga aaaaatttgc ggccaccaat ataagcttgc ggtataattt
     1441 cagataatct cggttcctct gatcgcctgc agaaatgcgg gcgatttgtt tttacgcatg
     1501 ggggtggaga aattaaaaag ataaaa
//
LOCUS       DATALY010000242         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_569939,
            whole genome shotgun sequence.
ACCESSION   DATALY010000242 DATALY010000000
VERSION     DATALY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569939"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VHR42_06765"
     CDS             complement(<1..1218)
                     /locus_tag="VHR42_06765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3026912.1"
                     /translation="MNIGKKTVRTAAAVLFALTVVGFGAVPAGIGASATTTGTPYNVT
                     AGIGTNETEVRFTWLSQGSSTGKLEIAKTSDMKNGSFPAGSEKQAATVKATATEGVLD
                     NPNNSDHPMGSFQDATGHALQDEYSSKVTVGGLSTNTSYTYRVGDGATWSKTYTFQTK
                     DPKGGFTFAAFGDPQIGASGNPDKDKAGWANTLSKVYARDPKLNFLFSMGDQVNDYDH
                     LYAQQKEYDNFFNPDFNKDYLQNHILAAFSGNHDFQMGKYYSFHYNQPNLSTLGQTTT
                     NGVADSNGDYWFRYGNTLFMQLEGNNFYDTAAHDSFLHQAISANPTAKWKVASFHQAP
                     YSEANHDGATTSDDDVMFMRKNWTKLMDKYGIDIVLNGHDHYYTRSFQMYGGSPVSTV
                     KTNQVTNPKGTVYF"
     gene            complement(1464..1976)
                     /locus_tag="VHR42_06770"
     CDS             complement(1464..1976)
                     /locus_tag="VHR42_06770"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX3026913.1"
                     /translation="MGTTGAALTMLRDRLLKRGFPMQAAYAVRMPGNYIRAYGAFSEK
                     EQQKLFEKEKPFARKIAEEIKAGKTNLRAAKANPLIRAISEQLNRSFRGKCTRSDENY
                     SVSEACVSCGLCERACPVHNITLEAGKPVWHHRCEACMACIERCPKQAINYKTSTVNR
                     KRYVNPHLKF"
     gene            2251..>2780
                     /locus_tag="VHR42_06775"
     CDS             2251..>2780
                     /locus_tag="VHR42_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026914.1"
                     /translation="MNTEMFHSVFHKAPILSRPGSSAKKLVCLLFVFLFLGADGLCKF
                     GASAAAYPKSFTARPATTVTPYRVYLSPSAQPWNPYCDGSGSEEDHMRQIAEAMIPYL
                     KGYGIESVLAAPSSGGRAAQRTTIVNRTQQAADSRCDLYLAIHSNAQEGILNEHWQEN
                     GPNSSCSSFCADGCRLR"
BASE COUNT          609 a          708 c          721 g          742 t
ORIGIN      
        1 aaagtatacg gtacccttcg gattcgttac ctgattggtc ttcaccgtgc tgaccgggga
       61 cccgccgtac atttggaagg aacgggtgta ataatggtcg tgaccgttga ggacaatgtc
      121 gatgccgtat ttatccatta gtttggtcca gttcttgcgc atgaacatca cgtcgtcgtc
      181 ggacgtggta gcgccgtcgt ggtttgcttc gctgtaggga gcctgatgga aggaagctac
      241 cttccacttt gctgtcgggt tggcgctgat tgcctggtgc aggaacgaat cgtgggcggc
      301 ggtatcgtag aagttattgc cctcaagctg cataaacagg gtgttgccgt aacggaacca
      361 gtaatcgccg ttgctgtccg ctacaccgtt ggtcgtggtc tgaccgagcg tggagaggtt
      421 cggctgattg taatggaaac tgtagtattt gcccatctga aaatcatggt ttccggagaa
      481 cgccgcaagg atatgattct gtaaatagtc tttgttgaaa tccggattga agaaattgtc
      541 gtattccttc tgctgagcgt agagatgatc atagtcgttg acctgatcgc ccatggagaa
      601 gaggaaattc agtttcgggt ctctcgcata taccttactg agcgtattgg cccaaccggc
      661 cttatccttg tcgggattcc cgctcgcgcc gatctgggga tcgccgaacg ctgcgaaggt
      721 aaagccgccc ttcgggtcct tcgtctggaa ggtataggtc ttactccagg ttgcgccgtc
      781 gcccacgcgg taagtataag aggtgtttgt ggaaagaccg cccacggtta ctttgctgga
      841 atattcgtcc tgcaaagcat gcccggttgc atcctgaaag gaacccatgg gatgatcgct
      901 gttgttggga ttatcgagga cgccttcggt agccgttgct ttcaccgtcg cagcctgctt
      961 ttcggagcca gccgggaagc tgccgttttt catgtcgctt gttttcgcaa tttccagctt
     1021 accagtggaa gaaccctggc tcagccaggt gaaacgcact tccgtttcgt tggtgccaat
     1081 cccggcggta acgttgtaag gagttccggt cgttgtggcc gatgctccga ttcctgcggg
     1141 aacagcaccg aagccgacaa ccgtcagcgc aaaaagaact gcagctgctg ttcggaccgt
     1201 tttcttgcca atgttcattt aatattccct cctcttgaat ggatggaatt attgtatccc
     1261 atttctgtat taatcaagtt actgccttgt taagcaaaat caaaattctg ttaaataaag
     1321 gtaaaattga tggcataaaa taggccccga ctcctccgcg ttgctgcggc ggggacggga
     1381 cctgaatgca agtgcctgaa agtgaaattt tccgacgatc ggggcaagag acgggagttg
     1441 gaagcaaaca gccgtacttc tcttcagaat ttcagatgag gattgacgta ccgcttccgg
     1501 ttaaccgtgc tggtcttata gttgatggcc tgcttcgggc atcgctcaat acaggccatg
     1561 caggcctcgc accgatggtg ccacaccggt tttcccgctt ccaaggtaat gttatgtacc
     1621 ggacaggccc tttcacacag gccgcaggag acacaggcct cgctgacgct gtaattttcg
     1681 tcggagcggg tgcattttcc ccggaaggag cggttcagct gctctgaaat agcgcggata
     1741 agcggatttg cctttgccgc cctcagattc gtcttcccgg cttttatttc ctccgcaatt
     1801 ttccgggcga agggcttctc cttttcaaaa agcttctgct gttccttctc ggaaaaggcg
     1861 ccgtacgccc ggatataatt tccgggcatg cggacagcat aggccgcctg cataggaaag
     1921 ccgcgtttca gaagccggtc cctcagcata gtgagcgcag cccccgttgt tccaattttc
     1981 ttttgtcata aataggatta ggagttttcg cgaaaaaagt cgtttttaca agtatttatt
     2041 tgaatttcca gtaataaaat gataaaatag aattggaaaa acctaaaatg tattcctttt
     2101 caatcgtctc tgtttctatc cggtttatca gctcctccga ttatgaggat ttgcaattat
     2161 tatcctcctt cctgagccgc ttgtatgcgg ctctttcctt gtcctttcca agcctgttcc
     2221 cattgaaagc cgaaaataca gaagaaagca gtgaatacag aaatgttcca ttccgttttc
     2281 cacaaagccc ccattcttag ccgcccaggc agttctgcaa aaaagctcgt ttgccttttg
     2341 tttgtttttc ttttcctcgg tgccgacgga ctttgcaaat tcggtgcaag tgccgcagct
     2401 tacccgaaga gctttaccgc acgtccggcc acgactgtaa cgccttaccg ggtctatctc
     2461 agtccttccg cgcagccgtg gaatccctac tgcgacggat ctgggagcga agaagatcat
     2521 atgcggcaga ttgctgaagc catgattccg tacctcaagg gatacgggat cgaatccgtg
     2581 cttgccgcac cgagttccgg cggtcgggcc gcccagcgca ccaccatcgt gaaccgcacc
     2641 cagcaagctg ccgacagccg gtgtgacctg tatctcgcca ttcattccaa cgcgcaggag
     2701 ggaatattaa atgaacattg gcaagaaaac ggtccgaaca gcagctgcag ttctttttgc
     2761 gctgacggtt gtcggcttcg
//
LOCUS       DATALY010000243         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_398019,
            whole genome shotgun sequence.
ACCESSION   DATALY010000243 DATALY010000000
VERSION     DATALY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398019"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHR42_06780"
     CDS             <1..691
                     /locus_tag="VHR42_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3026915.1"
                     /translation="KELSKTEELRRELIANISHDLRTPLTMVKMYAELIRDVSGDKPE
                     KRNAHTQVIIEESDRLSSLITEVLDLSKLQSGTAQISRKEFDLAEKSRVILNRFQALS
                     ERQGYVFSIDCDSDAWVNADEQKIEQVIYNLVSNAVNYTGEDKKVAIRVKKTGSKVRF
                     SVSDTGKGIPKEECGQIWERYYKAKETHKRAVVGTGLGLSIVKGILDAHNADYGVDST
                     VGTGSTFWFEL"
     gene            736..>1886
                     /locus_tag="VHR42_06785"
     CDS             736..>1886
                     /locus_tag="VHR42_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026916.1"
                     /translation="MSRQVKTVPELAPLRLINAAQRHVVKNLDRYTQTAVLITDRFYR
                     LLLDKNLPIIGINHRLKSAESLKEKILRKKLYKGSRTPEQIIRKITDMIGVMIECEFI
                     SDEEKIFSCIRDTFPLSEDGVFFYNPQFPDMMVDLGTPQPVKQKNGYELYKLDCRYAD
                     ENGEILFELQIKSLVNSFWCEVEHNIVYKNNHYISSDDYVNQMLSAVRINLAGLDRIL
                     QLISDRIGAMTAYNVVLDLRLNTSLTKQLVSDLINAKMVESVGFTMEAKRIRDLLAWY
                     ILEKLGPSDENSSAFFKLSGRFKELHQTEFRFDRQIELEYDLSSKNIFSRTVGGKMLQ
                     LMNSDFEWHVFFIMIFAIYAEQEPEEVFGEFIDTLNRIFCGGSYFKGLF"
BASE COUNT          505 a          427 c          505 g          449 t
ORIGIN      
        1 gaaagagctt tccaagacag aggagctgcg gcgggagctg attgccaaca tatcgcatga
       61 tctgcgcacc ccgcttacca tggtcaaaat gtatgccgag ctgattcggg atgtttcggg
      121 agacaaaccg gaaaaacgca acgcccacac gcaggtgatc attgaggaat ccgaccggct
      181 cagttcgctg attacggaag tgcttgactt gtcgaagctg cagtccggaa cagcccagat
      241 cagccggaag gagtttgatc tcgcggagaa atccagggtg attctcaacc ggtttcaggc
      301 gctcagtgaa cgccagggat atgttttcag catcgactgc gattccgacg cctgggtcaa
      361 tgccgacgag caaaaaatcg agcaggttat ttataatctg gtcagcaacg cggtaaacta
      421 cacgggagag gataagaagg tcgcgatccg ggtgaaaaaa acgggaagta aagtccgttt
      481 ttcggtttcc gacacgggga agggcatacc gaaagaggag tgtggacaga tttgggagcg
      541 ctattataaa gcgaaggaga cccataagcg tgccgttgtg ggaacaggcc tcggcctctc
      601 cattgtcaaa ggaatcctgg acgcccacaa tgcggattac ggggtcgaca gcaccgtggg
      661 aacgggcagc accttctggt ttgagctgta aaagctgaaa tttttaacgc aaggcaggcg
      721 ggaaaggatg tttcaatgag caggcaagtc aaaacagttc cggagctcgc gccgcttcgt
      781 ttaatcaatg ccgcccaaag gcatgtggtc aaaaatctgg accggtatac tcagacggcg
      841 gttttgatta ccgaccggtt ttatcgtttg cttctggata aaaatctgcc cattatcggc
      901 atcaatcatc ggctgaaatc cgcggaaagc ctgaaagaaa agatcctgag aaaaaagctg
      961 tataagggca gccggactcc ggagcagatc atcaggaaaa taaccgatat gatcggtgtg
     1021 atgatcgaat gcgaatttat cagcgacgaa gagaaaatct tttcctgcat cagggatacg
     1081 ttccctcttt ccgaggacgg tgtcttcttt tataacccgc agttccccga tatgatggtc
     1141 gatcttggaa cgccgcagcc ggtcaagcaa aaaaatggat atgagcttta taagctggat
     1201 tgccgctacg ccgatgaaaa cggagaaatt ctgtttgaac tccagatcaa atcattggtg
     1261 aattccttct ggtgcgaagt ggaacacaat atcgtctata aaaataatca ttacatttct
     1321 tcggatgatt atgtcaacca aatgctttcc gctgtccgaa tcaacctggc cgggcttgac
     1381 agaatcctgc agctgatcag cgaccgtatt ggagctatga ctgcctacaa tgttgttctg
     1441 gacctccggc tgaacaccag cctgaccaaa cagctggtca gcgacctgat taatgcaaaa
     1501 atggttgagt cggtcggttt cacgatggaa gccaaacgca tccgtgattt gctcgcctgg
     1561 tatattcttg agaagctcgg gccgtccgat gaaaactctt ccgccttttt caagctgtcc
     1621 ggccggttta aagaactgca tcagaccgag ttccgattcg accggcagat tgagctggaa
     1681 tatgatcttt cctccaaaaa tatattcagc cggacggtgg gaggaaagat gcttcagctg
     1741 atgaattccg actttgagtg gcatgtcttt tttatcatga tttttgcgat ttatgcggag
     1801 caggagccgg aagaagtttt tggggagttc atcgataccc tgaatcggat tttctgcggc
     1861 ggcagctatt ttaaggggct gtttga
//
LOCUS       DATALY010000244         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_658640,
            whole genome shotgun sequence.
ACCESSION   DATALY010000244 DATALY010000000
VERSION     DATALY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658640"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..818
                     /locus_tag="VHR42_06790"
     CDS             93..818
                     /locus_tag="VHR42_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEX3026917.1"
                     /translation="MRFKLIACGILKRELDQLVKRAGQQIDVTCLPQGMHNDPGELRG
                     ALQREIDRADSDPLGYDAILIGYGLCSNGPVGIRSGRYPLVIPRAHDCITLFLGSGHR
                     YRELFDQESGGIYWFTAAWIDQCPMPSRRSEEQRRNEIRQIYGSENIDFLLEAERSWV
                     KDYKCFAFIEFPGFREDPYREFTRKSADYFGLEFKNFRGDDSLLKDFLSGNWDHVRFQ
                     IVEPNQTLGPSYDDSIFTIEKRC"
     gene            885..1973
                     /locus_tag="VHR42_06795"
     CDS             885..1973
                     /locus_tag="VHR42_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026918.1"
                     /translation="MFQLPEYLPPDFSQPAFQNAPCVRIEPVPGDGQLPDEFYATTIY
                     PEYYKIDSQWRLLNAPRMDCAVVVKDGVPTAVEPRLVRAGDRVVVGRGENGNYGVFVD
                     YLGFSRAAEKKKDQSFAFRTGRSRESSYSRDYDMMYEILRHERDHGYIVWVLGPAVDF
                     DYDARRAMASLIRNGYVDALFAGNALATHDLEAALYHTGLGQDIYTKELMKNGHYHHL
                     EAINRARRAGSIAQLVQQEGIDDGIMAACVKTDVPFVLAGSIRDDGPLPGVIDNVYQA
                     QDAMRAHTRQATTVVALATQLHTIATGNMTPSWQLRDGKVRPVYFYTIDISEFAVNKL
                     RDRGSLSVRSIVTNIQDFLVHAAKNLID"
     gene            complement(2061..3056)
                     /locus_tag="VHR42_06800"
     CDS             complement(2061..3056)
                     /locus_tag="VHR42_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX3026919.1"
                     /translation="MFTFTMLLVIIFALSFDFINGFHDTANSIATSVSTRVLTPRMAI
                     IMSACLNFVGALMSEKVAGTISKGIVKGSLEEYVIISALAGAIIWNLITWFFGIPSSS
                     SHALIGSLMGAAAVYSGGFHIILWGDIIFKVIIPLFTSPLIGFIAGFIVMKFLYFILR
                     PFTHNTVSKLFSKLQIVSAAFMAYSHGSNDAQKSMGIITLALVSAGLHDPNIGVPIWV
                     KLICAISMAAGTSLGGWKIIKTIGVDMIKLHPIGGFAAETSSAAVIQVMTAFGAPVST
                     THVISSSIMGVGSSKRLSAVNWGIAKNIVQTWLLTIPSTMLLGAGVTYLVKLFVK"
     gene            complement(3472..4194)
                     /locus_tag="VHR42_06805"
     CDS             complement(3472..4194)
                     /locus_tag="VHR42_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3026920.1"
                     /translation="MISVKNLNKFFGKHQVLKDVNLEIEKGEKIVIVGPSGSGKSTLL
                     RCLNQLEVPSSGEIWLEGQLLTDPKCNINQMRRKMGMVFQHFNLFPHLSVMDNITLAP
                     MKLGLQTEKEAKENVERLLKRVGLLEKSNAYPSQLSGGQKQRIAIVRALAMNPDVMLF
                     DEPTSALDPEMVGEVLEVMKELANDGMTMAVVTHEMGFAREVGTRILFMDEGVILEQS
                     SPEDFFEHPQNPRLQDFLNKVL"
     gene            complement(4181..4882)
                     /locus_tag="VHR42_06810"
     CDS             complement(4181..4882)
                     /locus_tag="VHR42_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3026921.1"
                     /translation="MGIISSVWDRIGFNLIEKQRYLYLFNGLKITLIVTFFAVLLGIA
                     IGLLTAVVKVAAADNKKLRPFEILVNIYITVIRGTPVMVQLLIIYFVVFASVDVSKVL
                     IAIVAFGINSGAYVSEIIRAGILAVDKGQTEAGRSLGLSSSQTFRYIVMPQALKNILP
                     ALGNEFIALLKETAVVGYIGLQDLTMGGDIIRSRTYDAFVPLLTVAAVYLIIVMILTA
                     GLKRLERRLRQSDIR"
BASE COUNT         1303 a         1219 c         1349 g         1257 t
ORIGIN      
        1 gaagaaaagg ttttgaaaag aaacaggttt tcttgcaaaa ggcgtcggat cgagttaaaa
       61 taaattgttc ggattgggat gaggaggagc gtatgcggtt taagcttatc gcctgcggca
      121 ttttaaaaag agaactggat caactcgtta agcgggctgg tcagcagatt gacgttacct
      181 gtcttccgca gggtatgcac aacgaccccg gggaactccg cggcgcacta cagcgtgaaa
      241 ttgaccgcgc agactccgac ccgctcggct acgacgcgat actgatcggt tatggccttt
      301 gttccaacgg accggtcgga atccgttcgg gcagatatcc actcgttatt ccccgcgccc
      361 atgactgtat cacgctcttt ttgggctccg gccaccgtta cagggagctg tttgatcagg
      421 agtcgggagg aatctactgg tttaccgccg cgtggattga tcaatgcccc atgccgtccc
      481 ggcgaagcga agaacaaaga cgaaatgaaa ttagacaaat ttacggcagt gaaaatatcg
      541 attttcttct cgaggcggaa agaagctggg tgaaggatta caaatgcttt gcatttattg
      601 agtttcccgg tttccgggaa gatccgtacc gtgaatttac caggaaatcg gcggattatt
      661 ttggcctgga atttaaaaat ttcaggggag acgacagcct tttgaaagat tttctcagcg
      721 ggaactggga tcatgtccga tttcagatcg ttgagccaaa tcagacactt ggcccgagtt
      781 atgacgattc aatcttcacc atcgaaaagc gctgttaagc aagggccgta ggatacgcaa
      841 attattaaaa atgcagacaa aaatcaaaat aggggggatc actgatgttc cagttaccag
      901 aatatttacc accggatttc agccagccgg cgtttcaaaa cgcaccttgt gtcaggattg
      961 aacccgttcc cggcgacgga cagttgcccg acgaatttta tgccacgaca atttatccgg
     1021 aatattataa aatcgattct cagtggcgtt tgctgaacgc gccgaggatg gattgcgccg
     1081 ttgtggtcaa agatggcgtt ccgacagccg ttgagccccg tttggtgcgc gcgggggatc
     1141 gggttgtcgt cgggcgtggt gaaaacggta actatggcgt ttttgttgat tatcttggat
     1201 tttcccgcgc ggcggaaaag aaaaaggatc agtcctttgc gtttcgaacg ggccgctcca
     1261 gggaatcctc ctattccagg gattatgaca tgatgtatga aatcctaagg catgagcgcg
     1321 atcatggata tatcgtctgg gtgcttggcc cggcggtgga ttttgattat gatgcgagga
     1381 gggctatggc cagcctgatt cgcaatggat atgttgacgc cctgttcgcc ggcaatgccc
     1441 ttgcaaccca tgatctggag gctgcccttt accacaccgg gcttggtcag gatatctata
     1501 cgaaagaact gatgaaaaac ggccattacc atcaccttga agcgatcaac cgggccagaa
     1561 gggccggatc aatcgctcag cttgttcagc aggaaggtat cgatgacgga attatggcgg
     1621 cctgtgtgaa aaccgacgtg ccgtttgtgc tggcagggtc cattcgtgac gacggccctc
     1681 tgcccggagt aattgacaat gtctatcagg ctcaggatgc catgcgcgcc cacaccaggc
     1741 aagcgacaac ggtagtggcg ctcgcgaccc agcttcatac catcgcaacc ggtaacatga
     1801 ccccgtcctg gcagttaaga gacggaaaag tgcgtccggt ctatttctac acaatcgaca
     1861 tttccgagtt cgcagtcaat aaactccgcg accggggcag cctgagcgtc cggagtattg
     1921 tgacgaatat tcaggatttt ctggttcacg cggccaaaaa tttgattgac tgacattaga
     1981 ctgataaaag tcaattacag ggtttatacg gaactgaaac agcgcccgaa aaattcggac
     2041 gctgttttta tcaatttaat ttacttcaca aacagtttga ccagataggt gacccccgcc
     2101 ccaagcagca tggtggacgg aatggtcagc agccaggttt gaacgatgtt cttagcaatt
     2161 ccccagttga cggcggataa ccgcttggaa gaaccgacgc ccataatgga cgaggagata
     2221 acgtgggttg tgctgacggg cgcgccgaat gccgtcatga cttggatgac ggcggcagac
     2281 gaggtttccg ccgcaaaacc gccgatcggg tggagtttaa tcatgtcgac gccgatggtt
     2341 ttaatgatct tccagcctcc cagagaggtt ccggcggcca tggaaatagc gcagatgagc
     2401 ttgacccaaa tgggtacgcc gatgttcggg tcgtgcagcc ccgcgcttac gagagccagc
     2461 gtgataatac ccatggattt ctgtgcgtca ttgctgccgt gggaataagc cataaacgca
     2521 gcagatacaa tctgcagttt ggaaaacagt ttgcttaccg tattatgggt aaacggtctg
     2581 agaataaaat aaagaaattt catcacaatg aagccggcga taaaaccaat cagaggagaa
     2641 gtgaacagag ggatgattac tttaaaaatg atgtctcccc ataagataat gtgaaacccg
     2701 ccggaataga ccgcagccgc tcccatcagg ctgccgatca gagcgtggga ggaactgctg
     2761 ggaatgccga aaaaccatgt gatcagattc cagataattg caccggccag agcggagata
     2821 atcacatatt cctccaatga ccccttgaca atgcccttgg agatggttcc ggcaaccttt
     2881 tcactcatca gtgcgccaac aaaattcaga catgccgaca tgatgatcgc cattctcggg
     2941 gtcagcactc tggtggacac cgaggtggcg atggagttgg cggtatcatg gaaaccgttt
     3001 ataaaatcaa aagacagcgc gaagatgata accagcaaca ttgtaaacgt gaacataaat
     3061 cagtacagcc ctttcgatga cgtcttttaa cttttggagt tacggtgagt tatgcgttta
     3121 tgataggtat ctgtcatctt cttttgaaaa atttcaaggg taaaccgatt agcctttttt
     3181 cgcgaaaaga ctttttctgt tccctgattt tactcagaat ttacgaaatg aatacaatag
     3241 ttacgggatt tatacaaaaa agcacaatat aaagtatatt tgtttcacat gctgatgtca
     3301 agaaggatgg acgagatttc tgcatttgcc cgatggttgt aaagacaggg gcggacgggt
     3361 cagaaagagg gacaggattt aacctgcccc tctttctgct cggaatgtta aaatttgttg
     3421 cgccgcgggt gtccccggca gcgaataaga acctgccggt ccgatcggca tttaaagtac
     3481 cttgtttaaa aaatcctgga gtcttgggtt ctgcggatgc tcaaaaaaat cttccgggct
     3541 gctttgttcc agaatgacgc cttcgtccat aaataaaatc cgggtcccta cttcacgggc
     3601 aaatcccatt tcgtgggtga ccaccgccat agtcattccg tcattggcga gctccttcat
     3661 cacttcgagc acttcgccca ccatttccgg gtcaagggcg ctggttggct cgtcaaacag
     3721 cataacgtcc ggattcatgg ccagcgcgcg cacgatcgcg attctctgct tttggcctcc
     3781 ggataactgg gaaggatagg cgttggattt ttccagcaag cccactcgtt tcagcagtct
     3841 ttcgacgttc tctttggctt ccttttcggt ctgaaggccg agcttcatcg gagcgagcgt
     3901 gatattatcc attacgctca ggtgggggaa aaggttgaaa tgctggaaca ccatccccat
     3961 ctttcttctc atttgattga tgttgcattt tgggtcagtc agaagctgcc cttccagcca
     4021 gatttcaccg gaggacggta cctccagctg attcagacag cgaaggagtg tgcttttacc
     4081 cgacccagaa ggcccgacaa tgacgatttt ttcacccttt tcgatttcga ggttgacgtc
     4141 ttttagcacc tgatgttttc cgaaaaattt gttgaggttc ttaacggata tcactctgcc
     4201 tcagcctcct ttccagcctt ttgagacctg cggtcaaaat cataacgatg atcaggtaaa
     4261 ccgccgcgac agtcagcagg ggaacaaacg cgtcataggt gcggctgcgg atgatatcgc
     4321 cgcccatggt gagatcctgc aggccgatat acccgacaac cgcggtttcc ttgagcaggg
     4381 cgataaattc attgccgagg gcgggaagaa tatttttcag cgcctgcggc atgacgatat
     4441 agcggaaggt ctgggaagag ctcaggccca gtgaccggcc ggcctcggtc tggcctttgt
     4501 cgaccgcgag gataccggcg cggatgattt cggaaacata ggccccggaa ttgatgccga
     4561 atgccacgat ggcgatcaga accttcgaca cgtcgacgct tgcaaacacc acaaaataga
     4621 taatcaggag ctgcaccatg accggggtgc cgcggatgac cgtgatgtag atattaacaa
     4681 gtatttcaaa gggccggagc tttttgttat ccgccgcggc taccttaacg accgctgtga
     4741 gcaggccgat ggcgatcccg agaaggacgg cgaagaaggt cacgatcagc gtgattttca
     4801 aaccgttgaa caaatagaga taacgctgct tttcgatcag gttaaaaccg attcgatccc
     4861 agacagagga gatgatgccc atattattca cctttcaaca ggacaagaca gccgaaataa
     4921 cggctgtctt gtcaaaattg atcctgttgg ttttcagact ccgttacgaa tattttttga
     4981 tgatctgatc gatcgtgccg ctactcttga ggcgcgcgat cgtcttgttg atggattgca
     5041 gaagcttggt gttgcctttg cggacggcga cacagtattt ttccgaggtc aggggcttgc
     5101 cgacgatatt cagaccggga ttttgctt
//
LOCUS       DATALY010000245         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_658783,
            whole genome shotgun sequence.
ACCESSION   DATALY010000245 DATALY010000000
VERSION     DATALY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658783"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VHR42_06815"
     CDS             <1..597
                     /locus_tag="VHR42_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3026922.1"
                     /translation="RRFKGRGVEYDDLFQAGCLGLIKAVDHFDKERGVQFSTYAVPVI
                     LGEIRRLFRDGGAIKVGRGLKELSMRASREASAFTEREGRTPTVGELAEILQVEPEEA
                     AQALGAAQLPLSLTASQDDGGGQIDVSVDPADDKIAEILSLKQVVTELDPKDRSIIVF
                     RYFKSRTQTQTAQALGMTQVQVSRREKVILSQLRTKLS"
     gene            complement(823..1746)
                     /gene="metA"
                     /locus_tag="VHR42_06820"
     CDS             complement(823..1746)
                     /gene="metA"
                     /locus_tag="VHR42_06820"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEX3026923.1"
                     /translation="MPIKIPESLPATEVLESENIFVMTQQRAQTQDIRPLRIVILNLM
                     PTKIETETQLLRMLSNTPLQVDIELMQMATHVSKNTLASHLNTFYRVFDDLKDEKFDG
                     MIVTGAPVEKMPFEEVDYWNELCEIMAWSRKNVYSTLHICWGAQAGLYYHFGVPKYPL
                     EQKLSGIFRHYVIDPHHPLVRGFDDHFYSPHSRYTEVRREDIEQHKELVLLTASNAAG
                     VHIVANKNGRQFFVTGHLEYDRNTLANEYFRDVKKGLHPFIPQNYFPNDDVAQEPPFV
                     WRSPASLLFSNWLNYYVYQQTPYDLKDIGEN"
BASE COUNT          485 a          415 c          480 g          518 t
ORIGIN      
        1 aggcgattta aaggccgggg agtggaatac gacgatttgt ttcaggcagg ctgtctggga
       61 cttataaagg cggttgacca ctttgacaag gagcgcggcg tgcagttttc aacctatgcc
      121 gtgccggtaa tactgggtga aatccggcgc ctttttcgcg acggcggagc gattaaggtc
      181 ggccgcgggt tgaaggagct gtctatgcgc gcgtcccgcg aggcttccgc ttttacagag
      241 cgggagggga gaacgccgac ggtgggcgag cttgcggaaa tccttcaggt ggaaccggaa
      301 gaggcggccc aggccctcgg tgccgcgcag cttccgcttt ctcttacggc ttcacaggac
      361 gacggcggtg gtcagatcga cgtcagtgtg gacccggcgg acgataaaat tgcagagatt
      421 ctttcgttaa aacaggttgt gaccgaactg gatccaaaag accgaagtat tatcgtattt
      481 cgttatttta agagccgaac gcagacgcag acggctcagg cgcttggcat gacgcaggtt
      541 caggtttcgc ggcgcgaaaa agtgatactg agtcagcttc gcaccaagtt atcctagttc
      601 gataaagtca attattatgg gcataagccg ataggtttta atttgggttt gaaagaagcc
      661 ttaagttaat acccaatgca ttattgcctt cgcaaaggca atggaaagcg gcccaaattc
      721 agccggttag attccttcaa gggatagacc gtaattgact tttatataaa aggatctctt
      781 cacccaattg gatgaagaga tcctttaatt tttattcctg gttcagtttt ctccgatatc
      841 ctttaaatcg tacggtgttt gctgatatac ataatagttg agccagttgc tgaaaagcag
      901 gcttgcgggg cttctccaga caaagggcgg ctcttgcgct acgtcgtcat tcggaaagta
      961 attctgggga ataaacggat gcagaccttt ctttacatcg cgaaaatatt cgttcgcaag
     1021 tgtgttgcgg tcatattcca gatgaccggt tacgaaaaac tgcctgccat ttttatttgc
     1081 cacgatatgt actccggctg cgttggaagc tgtgagcaac acaagctctt tatgctgttc
     1141 aatgtcttcg cgccgtactt ccgtgtaacg ggaatgcgga gaataaaaat gatcgtcaaa
     1201 acccctgacc agcggatggt gcggatcaat cacgtaatgg cggaatatac cggagagctt
     1261 ttgctccagc ggatatttgg gaactccgaa atgataatac agccccgcct gtgctcccca
     1321 gcagatatgc agagtagagt agacattttt ccggctccaa gccatgattt cgcacagctc
     1381 gttccagtag tccacttcct caaacggcat tttttcaacc ggtgctccgg ttacgatcat
     1441 tccatcgaac ttctcatctt ttaaatcatc gaaaactcta taaaatgtat ttagatgaga
     1501 agctaatgta tttttagata catgggtggc catctgcatt aattcgatat cgacctgaag
     1561 aggagtgttg ctgagcattc gaagcaactg ggtttctgtt tcaatctttg ttggcatcag
     1621 attaagtatc acaattctca gcgggcgaat atcctgtgtt tgtgcgcgct gctgtgtcat
     1681 aacaaagata ttttccgatt ccagcacttc ggttgccggc aggctttcag gtattttaat
     1741 tggcatgctt ctgaccccct acacgagtat aatgcaacta ttatatcaaa catcttttct
     1801 agccgcaata agcgaaagcg gatgattgat gatgatttaa aaaatatgca gaaaaaggtt
     1861 gacattgcat ttttgaagtg gtattatagc aaagccgt
//
LOCUS       DATALY010000246         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_485332,
            whole genome shotgun sequence.
ACCESSION   DATALY010000246 DATALY010000000
VERSION     DATALY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485332"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VHR42_06825"
     CDS             <1..360
                     /locus_tag="VHR42_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX3026924.1"
                     /translation="SNDDSNQSVNTGTPGNQVVELKITSGPFSGKSCQAISPNVSNSG
                     AHCVPGLNVITRIVPRPDGQFSATVYNYDRGIFLWILIGLFFFLLCLTGGKKGITSSA
                     ALIFTFVCIIFLYIPLM"
     gene            361..984
                     /locus_tag="VHR42_06830"
     CDS             361..984
                     /locus_tag="VHR42_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX3026925.1"
                     /translation="MGVSPFWAASLAAAVITCMTMFLIGGWSSKTVCSIVGTLAGILA
                     AGLTAQLFGTLAHISGLNVSEFEVLAYIGMYSKLNVSEVLFSGILISSLGAVMDISMS
                     VASVVSEIHCTAPELSAKELFQSGMRVGRDMMGTMSATLILAYAGGSINTLIIIYAYS
                     MPYLQYINGYDIGINILSGISGAIGVILTVPFVSAVSAFIMTRREKR"
     gene            complement(1128..1661)
                     /locus_tag="VHR42_06835"
     CDS             complement(1128..1661)
                     /locus_tag="VHR42_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026926.1"
                     /translation="MTQKGKTTIRDITLVAVWAALTFAAKLVLAPIANVELVSLLLTV
                     FTVVMGLERGICAALVFTTLTVFESAYYGAGDWIVLYYINWPLLTLLSRAFLNKESSE
                     TRAAILLGLFGLLFDIPSTGIKLVLFGPGYAVAYLISGIPFDAIHGAANFISALFLYR
                     PLVRHLEKTRQHMEQTR"
     gene            complement(1658..2053)
                     /locus_tag="VHR42_06840"
     CDS             complement(1658..2053)
                     /locus_tag="VHR42_06840"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX3026927.1"
                     /translation="MKKKISLTLLVLAAAALLIAGYFQFLSPKAQAGAKSVTVQVVIQ
                     KNKINQTFHFTSSRSTVADLLKDEQANLKAVTENGKYGMFVSGMLGVKADSSKEYFNI
                     KVDGKDATVGVSELPLENGKTYLFTLTSL"
     gene            2322..2453
                     /locus_tag="VHR42_06845"
     CDS             2322..2453
                     /locus_tag="VHR42_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026928.1"
                     /translation="MASEWRTEVEDSIQAATGFPVTENEYTGIVPAYFERSFAARRR"
     gene            2567..3363
                     /locus_tag="VHR42_06850"
                     /pseudo
     CDS             2567..3363
                     /locus_tag="VHR42_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975793.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
BASE COUNT          843 a          836 c          857 g          827 t
ORIGIN      
        1 agcaacgacg actcaaacca gagtgtgaat accggaacac cgggaaatca ggtcgttgaa
       61 ttaaaaatca catccggccc gttcagcggc aaaagctgtc aggccatcag cccaaacgtc
      121 agcaattcag gagcgcactg cgtgcccggg ctgaacgtaa tcacacggat tgtcccccgg
      181 cccgacggcc agttttcggc aactgtttac aactatgacc gtggaatctt cctgtggata
      241 ttaattggac tgtttttctt tctcctgtgc ctaaccggcg ggaaaaaggg aattacttca
      301 tccgccgcat tgatttttac gttcgtctgc atcatctttt tatacatacc cctgatgtag
      361 attggagttt cgcccttttg ggccgcctct cttgccgccg cggtgatcac ctgcatgacc
      421 atgttcctca tcggcgggtg gtcgtcaaaa acggtttgtt cgattgtggg aacgcttgcg
      481 gggattctgg cggcaggact gacagcgcag cttttcggca cgcttgcgca catctccggc
      541 ttgaatgtct cagaatttga agttctcgcc tatatcggca tgtacagcaa actgaatgtt
      601 agcgaggttc tgttttccgg catcctgatt tcctcattgg gcgcggtgat ggatatcagt
      661 atgtccgttg catcggttgt ctctgaaatt cactgcaccg cgccggagct ttccgccaaa
      721 gagttgtttc aatccggcat gcgcgtgggc cgggatatga tgggcaccat gtcggccaca
      781 ctgattctgg cgtatgccgg gggatccatc aacacgctga tcatcattta cgcttacagt
      841 atgccctacc ttcaatatat caacggctat gacattggga ttaatattct gagcggaatt
      901 tctggagcca tcggcgttat tttaaccgtg ccctttgtat ccgcggtttc tgcattcatt
      961 atgacccgaa gggagaaacg gtaaaaagct ttgggctcaa tggctcttct aagatttcgt
     1021 actttattgt cccaatatta gcattataga gtgaaacagc acggattcct taagaggaat
     1081 ccgtgctgtt tttcacgcgg ttcatgcgca aaaaagcttc attcgcttca tcgagtctgc
     1141 tccatgtgct gccgtgtttt ttccaagtgc cgcacgaggg gacggtaaag gaaaagcgca
     1201 gagataaaat ttgccgcacc gtggatcgca tcgaacggta ttccgctgat cagataggcc
     1261 accgcgtaac ccgggccgaa cagcacgagt ttgatacccg tagaggggat atcaaacaga
     1321 aggccgaaca gaccgagcag aattgcagca cgcgtttcac ttgattcctt attgagaaac
     1381 gcgcgtgaca gcagagtcag gagcggccaa ttgatgtaat aaagaacaat ccagtcccct
     1441 gctccatagt acgcactttc aaaaacggtc agagtggtga aaaccagagc ggcacagatt
     1501 ccccgctcca gtcccatgac aacggtaaaa acagtcagca aaagcgatac cagctccacg
     1561 tttgcaatgg gggcaagcac aagcttggct gcaaacgtga gcgcggccca aacggcgacc
     1621 agcgtgatat cgcggatggt ggtctttcct ttctgcgtca taacgaggtc agggtaaaca
     1681 ggtacgtttt gccgttttcg aggggaagtt ccgacacacc caccgttgcg tcttttccgt
     1741 ctaccttaat gttgaaatat tctttggagg aatccgcttt cacgccgagc atcccgctta
     1801 cgaacattcc gtatttaccg ttttccgtaa cagccttcaa attagcttgc tcatctttca
     1861 gcaggtcggc taccgtcgaa cggctggacg taaaatggaa cgtctgattg atcttgtttt
     1921 tttgaatgac tacctgcacg gttaccgatt ttgcgcccgc ctgtgccttt ggcgacagga
     1981 attgaaaata ccccgcgatc agcagggcgg cggccgccag caccagcaga gtcaggctaa
     2041 tctttttttt catggaatca tatccctctc cgtaatgaat ttcctgatga atctaaagtt
     2101 tccgattgtt tcccattata cgatatctcg gcaaaagaaa gcaagaaata caaattttca
     2161 agagaatgcc aattgacggg ctgcgattta tactttataa ttaaaatcaa ctttatatta
     2221 aaaacacgac gacgaagaaa gtaggtcccc acgaacgcca aacagagatg tttccccgat
     2281 cattccgggc tgaaaggaaa caggcgcagc ggcgattgaa aatggcttcc gagtggcgca
     2341 ccgaggtgga agattccatt caggctgcga cgggatttcc cgttacggaa aacgagtata
     2401 ccggtatcgt tccggcgtac tttgaaagat cgttcgccgc gaggcggaga taaattgaag
     2461 tggtaacacg ggagcaattc tcgtcttctg attaggaaga cgggattttt ttatataaca
     2521 tcgtaaaaat atcgttggaa atgacggttg gataaaggag aacaccatga gaatcagcaa
     2581 actgttaaac agcgggaagg tcacggtttc gtgtgaagtt ttcccgccga aaaagacggc
     2641 ggatctggaa caggttcaga aggttgtacg gcaaatcgcg gcttttgagc cttcctttct
     2701 gagtgtcacc tacggtgcgg gcggcggaac ctcgcagaat acggtgaaac ttgccgccgg
     2761 ggttcagcag ttgggaattc ccgcgctggc acacctgacc tgcgcttccg ccacaaaaga
     2821 acaggtttcc gccgtgctgc aggagctgaa agaaaatcag attgaaaacg tgctcgcgct
     2881 gcgcggcgat atccccccgg gggtagaatt tccgactccg cggcagtatc ggtttgcctg
     2941 tgaactggtc cgtgaaatca aggaaaccgg ggatttctgc atcggcgcgg cctgttatcc
     3001 ggacggtcac gtggaatgcg agcacaaaga ggacgacatc gggtacctca agaaaaaagt
     3061 ggatgcgggc tgcgattttt ctgactacgc agatgttctt tgacaacaac gtgctttaca
     3121 attttctata ccgtattctg gctaagggaa ttcatatccc ggtggtagcg ggaatcatgc
     3181 cggtaacgaa cagcaaacag atcaaacgaa tctgttcgct ttcgggcacg gtcctccctc
     3241 cacgtttccg tgcgattgtc gataaattct ccgaaaagcc ggaagcgatg cagcaggcgg
     3301 gcatcgccta cgcaaccgag cagatcatcg acctgatttc aaacggtgta aacgccattc
     3361 acc
//
LOCUS       DATALY010000247         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_53933,
            whole genome shotgun sequence.
ACCESSION   DATALY010000247 DATALY010000000
VERSION     DATALY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53933"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..>2154
                     /locus_tag="VHR42_06855"
     CDS             37..>2154
                     /locus_tag="VHR42_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3026929.1"
                     /translation="MDAFPDWLKNDLNRNEWKIVDVGCAEGDGSAVLARNFPSCQVTG
                     MDFSESAVKNAKARFPFCNFIVGDATAVIEPTDVIFSSNTLEHLKNPRFILRNFCNSA
                     EKYAVILLPFEDNLGIAEHINIFFRDFFPVLLGKCYLKYYNVIDCAPMQSPYWPGKQI
                     LLVYVNPKYLPPEKMTVETIYRNYTYVWEQKSEYLSAKVEEQAKNIGELESAQSELKT
                     KIDALNKEKSVLLNSVTALKKAKQELKYKDDALNEENFKLIQQNNDLQQQNKQILARL
                     QANENAIRQAREFCYELAQTHLLKLVHFISRLKHQGIHGSKDDRKNFRRWFFSRFRHV
                     PDFNHRYNPIFGIIGILDSSAPAGESQNLFSADQSSKFVQHLNAQNAYLSEKISQPET
                     SQAKEIRKIIAERKYKGILVYPHVVYWEPLQTPQHLLRAFTKAGWLCFFCEHPNVSNA
                     FREIEPNLFIVYEADFLQAVKNAPVTVLLTWMGSMAFVGKIRNKTVWYHLMDHLEIFP
                     YFDQESLKMHNKMAKEADTISYVAKPLAKYFPDNAKAVYLPNGCCPDELLNLHKDYVP
                     DDLKKILATGHKIIGYYGYLAEWMDYDMVRAAALARPEYEFVFIGKAISDVSKIEGLP
                     NVHLLGLKPFPARCNRADQDNVALRKMRRYIADLLRIEPFRNRSIITDCLSAGIRCGT
                     KGLEKMFIFAAVPNDRAVFIIFRS"
BASE COUNT          614 a          472 c          502 g          566 t
ORIGIN      
        1 ggcgacaaac aatcagaatt tttttcacag gtcgcggtgg atgcttttcc ggactggctg
       61 aagaacgatt taaatcggaa tgaatggaaa attgtggatg taggctgtgc tgaaggagac
      121 ggcagcgcgg ttctggcacg caatttccct tcctgccagg tgactggtat ggatttttcg
      181 gaaagtgcag tgaaaaacgc aaaagcccga ttcccttttt gcaatttcat cgtgggtgat
      241 gcaaccgcag ttattgaacc tacagatgtg atcttttcat ccaatacttt agagcatctg
      301 aaaaatccac gcttcattct aagaaatttt tgcaactcgg cggaaaaata cgcggtgatt
      361 ttgctgccgt ttgaggacaa tctggggatc gccgagcata tcaacatctt tttcagagac
      421 tttttccctg tgctgttagg aaagtgctat ttaaaatatt ataatgtcat tgattgtgct
      481 cccatgcaat ctccctattg gccgggaaag cagatcctgt tggtttatgt aaacccaaag
      541 tacctgccgc ccgagaaaat gacggtggaa acgatctacc ggaattatac gtacgtatgg
      601 gaacagaagt cggaatatct gtctgcaaaa gtggaagagc aagcaaaaaa tatcggggaa
      661 cttgaaagtg cacagtcgga attaaaaact aaaatagacg ctttaaataa agaaaaatcg
      721 gtattgttaa attcggtaac cgccctaaaa aaagctaagc aggagctaaa gtataaagat
      781 gacgctttaa atgaggaaaa tttcaagctg attcaacaga acaacgactt acagcagcaa
      841 aataagcaga tattagctag gctgcaggca aatgaaaatg ccatccgaca ggcgcgcgaa
      901 ttctgctatg aattggcaca aacgcatttg ttaaaactgg tccatttcat cagtcggcta
      961 aaacatcagg gaatccatgg ttcaaaagac gatcgcaaaa atttccgccg gtggtttttc
     1021 tcgcgttttc gccatgtgcc ggatttcaat caccgctata atccgatttt tggaattatc
     1081 ggtattttgg acagctcggc gccggccggt gaaagccaga acctgttttc agccgatcaa
     1141 agcagcaaat ttgtccagca tctgaatgct cagaatgctt atctttcaga aaaaatatca
     1201 cagccagaaa ccagtcaggc gaaagaaatc cgtaaaatca tcgcggagcg gaaatacaag
     1261 ggaatccttg tttatcccca cgtggtctat tgggagccgc tacagactcc gcagcatctt
     1321 cttcgcgcct ttacgaaagc cggatggctt tgctttttct gcgagcatcc gaatgtttcc
     1381 aacgctttcc gggaaatcga gccgaacctc tttattgttt atgaagcgga ttttctgcag
     1441 gcggtcaaaa acgcgccggt tacggttttg ctgacgtgga tgggctctat ggcatttgtt
     1501 gggaagatca ggaacaaaac ggtctggtat cacctgatgg accatctgga aatttttcct
     1561 tatttcgatc aagagtcttt gaaaatgcat aacaaaatgg caaaagaagc ggatacgatc
     1621 agctatgtgg caaaaccgct cgctaaatat ttcccggaca atgccaaagc ggtttacctt
     1681 ccaaacggtt gctgcccgga tgagttgctg aacctgcata aggattatgt cccggatgat
     1741 ctgaagaaga ttctcgcaac cggtcataaa attattggat attacggtta tctcgccgaa
     1801 tggatggact acgatatggt gcgggccgca gcgttggcac gtccggaata tgaatttgtg
     1861 ttcatcggca aagcaatttc tgatgtttca aaaattgagg gcttgcccaa tgttcatctg
     1921 cttggtctca aaccgttccc tgcccgctgt aaccgtgccg atcaggataa cgtcgcattg
     1981 cggaagatgc gacgctacat agcagacctt ctccgcatcg aaccattccg caatcgctcc
     2041 ataataaccg attgtctttc tgccggtatc cggtgcggca ccaaaggcct tgagaaaatg
     2101 ttcatattcg ccgccgttcc gaacgatcgc gcagttttta ttatatttcg aagc
//
LOCUS       DATALY010000248         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_659313,
            whole genome shotgun sequence.
ACCESSION   DATALY010000248 DATALY010000000
VERSION     DATALY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659313"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VHR42_06860"
     CDS             <1..360
                     /locus_tag="VHR42_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX3026930.1"
                     /translation="YDLAVKTLNPRKLSRSSYAGSAAAAILTDKGNVYTGVCIDTPSS
                     LGFCAEHAAIAAMVTAGESRIVRLVAVLEGGKAGAPCGRCREFICQIDDENGKCEVML
                     DHGKITTIDELLPYRWN"
     gene            complement(757..>1980)
                     /locus_tag="VHR42_06865"
     CDS             complement(757..>1980)
                     /locus_tag="VHR42_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3026931.1"
                     /translation="RYPRSTLRPELSVRYLDFSVSRDQSAVRFAVLLSNGEKTKTLLA
                     ALFLKEISFLLIDEPTNHLDIQSRGLVSRYLRQKKGFILVSHDREFLDGCIDHILSMN
                     RTNIVIQKGNFSTFLQNKRKEDDFENAANDKLKKEIERLSNTAKRTADWSRETEKSKY
                     RTLNSGLKVDRGYLGRKSAKMMKRSKSLENRQQKAIQEKSELLKNVETAESLKISPLV
                     YHSRLLAEFRGISIHYGPATVCNDVGFTVRQGERIALCGKNGSGKSSLLKLLNGEPMD
                     YTGILEIGSGLVVSYLPQDTSFLSGSLKKFARDSGVEESLFLAILRKLDFSRAQFEKD
                     LADYSEGQKKKVALARSLCEKAHLYLWDEPLNYVDVLSRIQIEEILVNYRPTMVFVEH
                     DRAFVRNVATKIFEL"
BASE COUNT          494 a          489 c          454 g          543 t
ORIGIN      
        1 tatgatttgg cggtaaaaac gctgaaccca agaaaactgt cccgcagctc atatgcggga
       61 tcggccgcag cggccatttt gaccgacaag ggaaatgttt acaccggtgt gtgtatcgat
      121 accccgagct ctctgggatt ctgcgccgag catgccgcca tcgcggcgat ggttacggcg
      181 ggagaaagca ggattgtgcg gctggtagca gttctcgagg gagggaaggc cggagcgccc
      241 tgtggaagat gcagagaatt tatctgccag atcgacgatg aaaacggcaa atgtgaagtg
      301 atgctcgatc atggaaaaat tacaacaatt gatgagcttc ttccttatcg ctggaactga
      361 tcggggcagg gcagaaggat agattcaaaa aggaaagaga gtctcctttg gttcgtgcgg
      421 tcaatcccgc tgacagtcaa ggaagactct cttttttttg tgcgtttcgt ttgcaaacat
      481 atcctcgtct atcactttcc tgccgacaca ccctggctgg cgaccttctg cattttcgcc
      541 ctcaccagtt cctgactgcc aagatagtat tttcttaaga tgttccattt atcgtcaaat
      601 catataactc ttcaaaactc attttttatt cctccttgca aatgacgcat tcacaaaccg
      661 gattttcttt aattttatat taacataaaa tttcaaaaag cgcacggaaa gatcactttt
      721 tcctaaggcc gtctttgggt cagacgtcga tatgtctcac agttcgaata tttttgtggc
      781 gacatttcgg acaaacgccc ggtcatgctc cacaaaaacc atggtcggac ggtaattcac
      841 aaggatttct tcaatctgaa tccgggataa tacgtcaaca taattgagcg gctcatccca
      901 aagatacaga tgcgcttttt cacaaaggct tctggccagc gcaacctttt tcttttggcc
      961 ttcgctgtaa tcggccagat ctttttcaaa ctgagccctc gaaaaatcca gttttcgaag
     1021 aatcgccagg aaaaggcttt cctccactcc gctgtccctc gcgaacttct ttaaactgcc
     1081 ggataaaaac gacgtatcct gcggaagata ggaaacaacc agaccgctgc cgatttccag
     1141 aatgcctgta tagtccatcg gttccccgtt cagcaacttc aaaaggcttg atttcccgct
     1201 tccgttcttc ccgcaaagcg cgatgcgctc tccctgacgg acggtgaaac caacgtcgtt
     1261 gcaaaccgtg gccggcccat aatgaatcga aattccgcga aactcggcca gcaggcggga
     1321 atgatagaca agcgggctga tcttcaggct ctcggccgtt tccacgtttt tgagcagttc
     1381 cgatttttcc tgaatcgcct tttgctgccg gttttccagc gatttcgacc gtttcatcat
     1441 cttagccgat ttgcggccaa gatacccccg gtcaacctta agaccggaat taagcgttcg
     1501 atatttggat ttttcggtct cccgagacca atcagccgtc cgcttcgcag tattgctcag
     1561 ccgttcaatt tcttttttga gcttgtcatt tgccgcattt tcaaaatcgt cttctttgcg
     1621 cttgttttgc agaaaagttg aaaaattgcc tttttgaatc acgatattcg tccggttcat
     1681 ggaaagtata tgatcgatac agccatcaag aaattcccgg tcatgcgaaa cgagaataaa
     1741 tccttttttc tgcctgaggt aacggcttac caaaccgcgg ctctgaatat caaggtgatt
     1801 ggttggctcg tcgatcagga gaaagctgat ttcttttaaa aagagggcgg caagcagagt
     1861 tttggtcttt tcaccgttgc tgagcaatac tgcgaagcgg acggctgatt ggtctcggga
     1921 gaccgaaaaa tccaaatatc gaacgcttaa ttccggtctt aaggttgacc gggggtatct
//
LOCUS       DATALY010000249         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_315062,
            whole genome shotgun sequence.
ACCESSION   DATALY010000249 DATALY010000000
VERSION     DATALY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315062"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VHR42_06870"
     CDS             <1..258
                     /locus_tag="VHR42_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX3026932.1"
                     /translation="ASYIATKSGLVIMADINSQKKFADGSEKPAPYEAFTRPWYIKAK
                     AEQKQVFTDVIYDVHGGGLAIVCAQPFFRNGEFMGVAGVGS"
     gene            complement(255..815)
                     /locus_tag="VHR42_06875"
     CDS             complement(255..815)
                     /locus_tag="VHR42_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026933.1"
                     /translation="MIPTKKRSIRFKVVMLVLIVSGIVLGTLCISMLFTMNTMRNSAV
                     HTNQTLGSMAAKDAKTALSEQAKQNISELARSKADYIEQRFNITETYDSQIADEMTRI
                     YGNSAAYPDRNVAEPVKENAGVLKAQLMYSAIVKDQASVSEELAKAANIQDLLYQVVN
                     HDKMIGASYIATKSGLVIMADINSLS"
     gene            complement(812..1834)
                     /locus_tag="VHR42_06880"
     CDS             complement(812..1834)
                     /locus_tag="VHR42_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026934.1"
                     /translation="MEVKLKHRTNSSELTVILRPFEDGDADDVIACIRDEYGDTYFKR
                     NFYNSEFLKKMHREGAITFLVAQTDWGEIAGILALKSFYPAETMCEVASEIFRKKFRG
                     YRLAEPMLRYGLDIIAQGSYSAAYALPVTFHRITQQILQNLGMTATGFIFSVFRTECM
                     ESSYDYGGCQKHSQGIQIMPMEKTDTGSLYLPEELTSIAELIYSRLGVDYEICHAETS
                     LKAKTRLYFTNDTLHRNGTIMLLRPGADLKLQVDQVRARYQGVPLQTFNVFLNISDPA
                     APEAYRILKESGFFFTGFKPLCSEREYLVMHDANGVAPHTEDYALSNEFEELLDDISP
                     FIKVRG"
     gene            complement(1934..>2591)
                     /locus_tag="VHR42_06885"
     CDS             complement(1934..>2591)
                     /locus_tag="VHR42_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026935.1"
                     /translation="LLANLLTLLRALALVVLFAAMFAGTGNLTGVWASFLAADIATFG
                     ILLAAAALIRRKNPALTGSLLLNTVYERCGRYISFAVEGNPDAAAEAAEKLGGFCQEN
                     ALDRRTSMKLSLSVEEMLVCICEHCFAGGEQPIDVRIFIMDDMVILRIRNNGNIFDPV
                     SYYEKKAEEADGGILTDDSLGIGMIVKKAVSVKFNRTFGVNNLTILLHLKAKASASPA
                     "
BASE COUNT          652 a          663 c          600 g          676 t
ORIGIN      
        1 gcctcatata ttgccacaaa atccggcctt gtcattatgg ccgatataaa ttcacagaaa
       61 aagtttgccg atggctctga aaagccggca ccctatgaag cctttacccg cccgtggtat
      121 atcaaagcga aggcagagca gaaacaggtt ttcaccgatg tcatatacga cgtgcacggc
      181 ggaggcctgg ccattgtctg cgcccagcct ttttttagaa acggcgagtt tatgggggtt
      241 gcgggagtgg ggtcctaact cagtgaattt atatcggcca taatgacaag gccggatttt
      301 gtggcaatat atgaggcccc aatcatcttg tcatgattga caacctggta aagcaaatct
      361 tgaatattgg cagccttggc gagctcttcc gaaacacttg cctgatcttt cacaattgca
      421 gaatacatta gctgcgcttt aagaacgcct gcattttcct taaccggttc cgcaacgttg
      481 cggtcaggat aggcggcgga atttccataa atccgggtca tttcatccgc aatctgactg
      541 tcataagttt cggtaatgtt gaaacgctgc tcgatataat ccgctttgct gcgcgcaagc
      601 tctgagatat tctgtttggc ttgttcggaa agagcggttt tagcatcctt ggcggccatg
      661 cttcccagtg tctggttggt gtgaaccgca ctgtttcgca tggtgttcat ggtaaaaagc
      721 atacttatgc ataacgtgcc cagtacaatt cctgacacga tcagcaccag catgactacc
      781 ttgaagcgta tgcttcgctt tttcgtcgga atcatcccct aactttgata aaaggactga
      841 tatcgtccag aagttcttcg aattcattac ttaaggcata gtcctccgta tgcggtgcaa
      901 caccgtttgc gtcatgcata acgagatact ctctctcact gcagagcggt ttaaaacccg
      961 tgaaaaaaaa tccgctttct ttcagaatcc ggtacgcttc cggggcggca gggtcgctga
     1021 tattcaaaaa cacgttgaag gtttgaagcg gtactccctg ataccttgct ctaacctggt
     1081 caacctgcag cttcaaatcc gcccctggcc gcagaagcat gatagttccg ttcctatgca
     1141 gcgtgtcgtt ggtgaagtat agccgtgttt tcgccttaag tgaagtttcc gcgtgacaaa
     1201 tttcataatc caccccaagt ctgctataga tcagttcggc aatagaggta agctcctccg
     1261 gaagatataa gcttccggtg tctgtctttt ccataggcat tatctgaatt ccctgcgaat
     1321 gcttttggca tcctccgtaa tcatacgagg actccatgca ctcggtgcga aacacggaga
     1381 aaataaagcc ggttgccgtc atgcccaggt tttgtaaaat ctgctgagta atcctgtgaa
     1441 aagttacagg tagggcatag gcagcagaat agctgccctg agcaatgata tccaagccat
     1501 agcggagcat tggttcggca agacggtagc cgcggaattt cttccggaaa atttcggaag
     1561 ccacttcaca catggtttcg gctgggtaaa agcttttcag ggcaaggata ccggcaattt
     1621 ctccccaatc ggtctgggct acaagaaagg ttatggcgcc ttccctgtgc atttttttga
     1681 gaaactcgga attataaaaa tttcttttga aataagtatc accgtattca tcgcggatac
     1741 aggcaatgac gtcatcggcg tccccatcct caaagggccg caggatgaca gtcaactcac
     1801 tggaatttgt tctatgtttt aattttactt ccaattttta atcctctctt acagcaattc
     1861 aaattaagcc gcgaagcgga aggtgttctc gcagccctgc gggcagaggc ggcgcaatct
     1921 caaacattct gcatcaggca ggagatgctg aagcttttgc ttttaaatgc agcagaattg
     1981 ttaaattgtt cacaccgaag gttcggttga acttcacaga aacggctttt tttacaatca
     2041 tacctatccc caggctgtca tccgtaagaa tcccgccgtc ggcctcctcc gccttcttct
     2101 cataataact gaccggatca aaaatatttc cgttgttgcg tatgcgcaaa atcaccatat
     2161 cgtccatgat gaagatccgc acgtcgattg gctgttcccc gccggcaaag cagtgttcgc
     2221 agatgcagac cagcatttcc tctacggata gcgacagctt catggaggtt cgcctatcca
     2281 gcgcattttc ctggcaaaac ccccccagtt tttccgcagc ttcggcggcg gcgtctggat
     2341 ttccctccac tgcaaaggat atgtaccgcc cgcagcgctc atacaccgta ttcagcaaga
     2401 gactaccggt aagcgcagga ttttttctgc gtatcagcgc ggcggccgcc agcaggatgc
     2461 caaaggttgc tatgtcagca gccaaaaagg aggcccacac acccgtcagg ttcccagttc
     2521 cagcgaacat tgccgcaaaa agcaccacca acgctaaggc ccgcaaaagt gtcagaagat
     2581 tcgccagcaa a
//
LOCUS       DATALY010000250         5698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_229605,
            whole genome shotgun sequence.
ACCESSION   DATALY010000250 DATALY010000000
VERSION     DATALY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229605"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            608..2464
                     /locus_tag="VHR42_06890"
     CDS             608..2464
                     /locus_tag="VHR42_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026936.1"
                     /translation="MFQTLKGKISAVYIGLVFLIAAVSCVSVLNLIDLERSVNGLMTR
                     NYQSISYAQEMLDALAQQNLAVVTFLSVDNDSGIDRFYSHGGDFFRYLNMEAHNITEK
                     GEQSIVDRVFTSYNEFQKANTRLAEIKSGKGKAEAYRFYNAQITPKVTEIQNQIKSLI
                     ALNQTAMFNSKDRASEKARFSVYALLCLSFAAVLGGFLVSGYFINRFLLPLRQLTEGI
                     NRVRAGELDLKLNIRTNDETGRLAREFNEMTGRLSVYEQSTMGSLVSERNKSVAIVRS
                     ISDPLIVLDVNYRIMLINHACESFFTISEQEVVGKHFLEAVHRGELFDLVAESAEYPD
                     GHTEKIIPFYGEENTYYNVVITRIFDSDQKPSGFILLMQNVTELKELEKMKTDFMATV
                     SHELKTPLTSILMGASMLEDKNLGVLNSGQWDVVRTITEDTGRMADFVSELLEISRIE
                     SGKAIYSFKPCSLEAISESSCHRFADAAGRQGVELKNMVSDSLPLVYADFEKVTWVIN
                     NLLSNALKYTDERDSITISAERDGDFLRVAVTDTGEGIPQEYLERIFEKFVRVRERDI
                     ELRGTGIGLSVSKELITAHRGKIWAESEPGQGSTFYFTLPIYFESGGEAEEK"
     gene            2461..3054
                     /locus_tag="VHR42_06895"
     CDS             2461..3054
                     /locus_tag="VHR42_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3026937.1"
                     /translation="MKVLIVDDTKNIRMLLLRCLEMEGYDVQTAGDGKTALELFTKER
                     FDLAFLDIKMPHFSGTEVLKRVREMGIRTPVIIITAYATVKNAVECTNLGSVAYLQKP
                     FSAEKIKSVLNEILKFNSGESSLENILNLARSEVESGRAEKAEKILRRAVSDYTLSPE
                     VYQLLSRVCEVRQNRKDALKYRKIFEALTPAADTDGE"
     gene            3134..3781
                     /locus_tag="VHR42_06900"
     CDS             3134..3781
                     /locus_tag="VHR42_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3026938.1"
                     /translation="MIIKWYGQSCFLITTGDGEKIVTDPFNGMLGYRVPSLEADLVTV
                     SHQHSDHNNLAAVRGADRLVGDAGVIRIGKVDVNGIPSFHDRKSGRLRGENLIFNIEA
                     DGIHVCHLGDLGHELSPEQAAQIGPVDILLIPVGGFFTINGQTAAQVAAMLHPSIVVP
                     MHYRTKAVRLRMPMISTEEPFLEAMGTEAETLSRLEILRQDLPQKLRCVVLDYNS"
     gene            complement(3782..4615)
                     /locus_tag="VHR42_06905"
     CDS             complement(3782..4615)
                     /locus_tag="VHR42_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX3026939.1"
                     /translation="MFRYAFMQNALAVSLLISILCPCIGVFLVLRRYSMIGDTLSHAS
                     LAGIAIGLLSGHSPIVGAFVFTSVCGALIELLRESFKKYTDLILTVVLSLSVGTAITL
                     MSSGKLHANINSFMFGSILTVSRADLYTVFVLSIVSVLTVIFLYNQLVFITFDEEAAK
                     IAGVRVRLINYVFSILVAAAIAVSIRIVGVLVLSSMIALPVATALQLQKGFKVTLLAS
                     IAFSVVDIMLGLLLSYEAGCAPGGLTALISVFLLLIVIVIQKARGILRLKSSGQASRR
                     A"
     gene            complement(4608..5285)
                     /locus_tag="VHR42_06910"
     CDS             complement(4608..5285)
                     /locus_tag="VHR42_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX3026940.1"
                     /translation="MIHLKNLYYSYTGTEPFLLRNISLEIKNGEYLSILGENGSGKST
                     LIKLILNLLHPVGGTIENQAKKTGYVPQKADDLNAGFPITVTEMLNCYRRLIKVKDRG
                     TIRRCLETVRMQDFGSALIGTLSGGQRQKIYIARALMGNPDLLILDEPSTGVDIGSQA
                     EIYALIKQLNTEKGITVVSVEHNLEAAVSNSTQIYHLSDGGGHLCAPDKYISEFINSN
                     RGTKKDV"
     gene            5447..>5698
                     /locus_tag="VHR42_06915"
     CDS             5447..>5698
                     /locus_tag="VHR42_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX3026941.1"
                     /translation="MIRKFFAFFLIIGLFLTLSACSEPVKKKSGGKVGVVVTFNALAE
                     FARAVGGNRISLTTIIPDGVEPHDFEPKVKDMKLISDADV"
BASE COUNT         1448 a         1294 c         1550 g         1406 t
ORIGIN      
        1 agtaatcatt tggttaaaat aaaaaagaca aagcgcgaag ctttgctctg gttaaaatga
       61 ttggtgaaat atttcattgt cttgcaagta ttataatcca atccgcaggg ggaatggaaa
      121 ccgccggaat gggcggatgg ctttttacca tgcatgctac gggcggtttt aggcaagggc
      181 ggctcgttat tgcagatttt cttattgttt cgtttaaatt ctatgcagtc tatttattgt
      241 gcaaaggaat aaaaaagtat accttataag ggaagcgttt tcaggaggag acgttatact
      301 catactgaat cacgcagagt atgacggtga tggctagaag cgtgtgcaga acaagcgcca
      361 tgggaaactc ctcccttaat ttacggttga aaccggattt gttggggatt ctcttcctga
      421 aaattataat acgttccgtc ggaaaacgaa aattcttcat ccggtgggct atgggctctt
      481 gcggagctgt tgtcggccgt ttttatcaac ggcggcgtcc gttcgtatca ttttttcagg
      541 aatcggtccg ttttgaaacg agatcgattt tatcgtttcc cagtaatatc gtcatggagg
      601 tcataggatg tttcaaaccc tgaagggaaa aatatcggcg gtttatattg ggcttgtttt
      661 tctgatcgcg gcggtcagct gcgtgtcggt tctcaatctg attgaccttg aacgatcggt
      721 taacggcctg atgacgcgta attatcagag catctcatat gcccaggaaa tgctggatgc
      781 tctggcgcag cagaatcttg cggttgttac cttcctcagc gtcgataacg atagtggtat
      841 cgaccggttt tacagtcatg gcggagattt tttccgatat ttgaacatgg aggcccacaa
      901 cattaccgaa aagggcgagc agagcattgt ggaccgggtt tttacaagct ataatgaatt
      961 tcagaaagcg aatacccggc ttgcggaaat aaaaagcggc aaagggaaag cggaagcata
     1021 ccgtttttat aacgctcaga tcacgccaaa ggtgacggaa attcaaaacc agatcaaaag
     1081 cctgattgcg ctcaatcaga ccgcaatgtt caacagcaag gacagggcgt cggaaaaggc
     1141 ccggttttcg gtgtatgcgc ttctgtgtct gtcgtttgcc gccgttttag gaggttttct
     1201 ggtttcgggg tatttcatta accggttttt gcttcctctc cggcagctga ctgagggaat
     1261 caaccgggtt cgggccggag aacttgatct gaaactgaat atccggacaa acgatgaaac
     1321 ggggcggctt gccagggagt ttaacgagat gaccgggcgg ctttcggttt atgaacaaag
     1381 caccatggga agcctggtca gcgagcgcaa taaatcggtc gccattgtca ggagcatctc
     1441 cgatccgctg atcgttctgg atgttaatta ccgaatcatg ctgatcaatc atgcgtgcga
     1501 atcctttttc acgatctccg agcaggaggt tgtcggcaag cattttcttg aagccgtcca
     1561 tcggggtgag ctttttgatc tggtggccga gagtgcggaa tatcccgacg ggcatactga
     1621 aaaaataatt cctttttatg gggaggaaaa tacttactac aatgtggtca ttacccggat
     1681 ttttgactcg gatcaaaagc cgtctggctt tatactgctg atgcagaacg taaccgaact
     1741 gaaagaattg gaaaaaatga aaaccgattt tatggcgacc gtttcccatg aattgaaaac
     1801 tccgctgacg tcgattctca tgggtgcgag catgctcgag gacaaaaacc ttggcgtgct
     1861 caattcgggc cagtgggatg tcgtcagaac cattacggag gataccggca gaatggcgga
     1921 ttttgtcagt gaactgcttg aaatttcgag aatcgaatcg gggaaagcga tctattcgtt
     1981 taaaccgtgt tcgcttgagg cgatttccga gagttcctgc caccggtttg ccgacgcggc
     2041 cggaaggcag ggtgttgagc tgaaaaacat ggtttcagac agccttccgc tggtttacgc
     2101 ggacttcgaa aaggtgacct gggtaatcaa caatcttttg agcaatgcgc tcaaatatac
     2161 cgacgagcgc gattccatca cgatttccgc cgagagggac ggagattttc tcagggttgc
     2221 ggtcaccgat accggggaag ggattccgca ggagtacctc gagcgtattt tcgaaaaatt
     2281 tgtccgggtc agggagcggg atattgagct gaggggcacc gggatcgggc tgtcggtttc
     2341 caaagagctg attaccgccc accgggggaa aatctgggcg gagagtgagc ccggccaggg
     2401 cagtacattt tattttacgc ttccgattta ttttgaaagc ggcggggagg cagaagaaaa
     2461 atgaaggttt tgattgtcga cgataccaag aatatccgca tgctgctgtt aagatgccta
     2521 gaaatggaag ggtatgacgt acagacggcg ggggatggca aaaccgctct tgagctgttc
     2581 acaaaagagc ggtttgatct tgcttttctt gatataaaaa tgccgcattt cagcggaacg
     2641 gaagtgctca aacgagtccg ggaaatggga atcagaactc cggttattat catcaccgct
     2701 tacgctacgg tcaaaaatgc ggtcgaatgc acgaatcttg gctcggtcgc ctatctccag
     2761 aagccgtttt cagcggaaaa gatcaaatcg gttctaaatg aaatcctgaa attcaattcc
     2821 ggtgaaagca gccttgaaaa tattctgaac ctcgcccggt cggaagtgga gtccggcagg
     2881 gcggagaagg ccgaaaaaat actccggcgg gcagtgtcgg attacacact gtctcccgaa
     2941 gtttatcaat tgctctcacg ggtctgtgaa gtgcggcaaa atcgaaaaga cgccctgaaa
     3001 tatcgaaaga tttttgaggc tttgacgcct gcggccgata cagacggaga ataagagaat
     3061 caaagaatca cgcagcctgg tcttccggca gcgagccgga ggagtacgga aatttgaaag
     3121 gggcgctaag tttatgataa tcaaatggta tggccagtcc tgctttctga tcacgacggg
     3181 cgacggagaa aaaatcgtca ccgatccgtt taacgggatg ctcggttacc gcgttcccag
     3241 cctcgaagcg gatcttgtga ccgtaagcca ccaacattcc gaccataaca atctggcggc
     3301 cgtccgaggg gccgaccgcc ttgtgggcga cgccggtgtc atccgtatcg ggaaagttga
     3361 cgtcaatggg atcccgtcct tccacgaccg aaaaagcggg cggctgagag gtgaaaatct
     3421 tatttttaac attgaggccg atggaataca tgtctgtcat ttgggcgatc tcggccatga
     3481 gctttccccg gagcaggccg ctcaaatcgg cccggtagac attttgctga ttccggtggg
     3541 cgggtttttc accattaacg gccaaacagc tgcccaggtg gctgctatgc ttcacccgtc
     3601 aatcgtggtt ccgatgcatt accggacgaa ggcggtgcgc ctgagaatgc cgatgatcag
     3661 cacggaagaa ccctttcttg aggcaatggg gacggaagcc gagactctca gtcggctgga
     3721 aattctgaga caggatcttc cgcaaaaact gcgctgtgtg gtgcttgact acaattcgta
     3781 gctatgcccg gcgggacgct tgcccggagc ttttgaggcg gagaatcccc cttgcttttt
     3841 gaatcacgat cacaatcagc agcaaaaaaa ctgagatcag tgcggtcagg ccaccgggag
     3901 cgcaccctgc ttcataggac agcaggaggc cgagcataat gtcgacgacg ctgaaagcaa
     3961 tcgatgccag cagagttact ttgaaacctt tctgcagctg gagggccgtc gccacaggca
     4021 gggcaatcat ggagctgaga accagcacgc cgacgatccg aatggaaacg gcgatggctg
     4081 cggccaccag aatggaaaag acgtagttga tcagccggac tcttaccccg gcgattttcg
     4141 ccgcctcctc gtcaaaggtg ataaagacca gctggttata aagaaaaatg acggtaagca
     4201 ccgaaacaat gctcaggaca aaaaccgtat aaaggtcggc tcttgaaacg gtcaggatgc
     4261 ttccaaacat aaacgaattg atattggcgt gaagttttcc ggagctcatc agcgtgattg
     4321 cagttccaac gctcagggac agcaccaccg tcagaatcag atcggtatat tttttaaagg
     4381 attcccgcag cagctcgatc agcgcgccgc agaccgatgt gaacacaaat gcgccgacga
     4441 tcgggctgtg gccgctcaga aggccgatgg cgataccggc gagcgaggcg tgggaaagag
     4501 tgtcgccgat catcgaatac cgcctgagga cgaggaagac gccgatacag gggcatagaa
     4561 tcgagattaa aagggaaact gccagcgcgt tttgcatgaa tgcgtatcta aacatctttt
     4621 ttggtccccc tgttggaatt gataaattca ctgatgtatt tatccggcgc gcagagatgg
     4681 ccgccgccgt cggacaaatg atatatttgc gttgaattgg agaccgcggc ctccaggttg
     4741 tgttcaaccg aaacgactgt gattcccttt tccgtgttca gctgcttaat cagtgcgtag
     4801 atttcggcct ggctgccgat gtcgaccccg gttgacggct cgtccagaat cagaagatcg
     4861 gggttgccca tcagggcccg cgcaatatag attttctggc gctgtccgcc cgaaagcgtt
     4921 ccgatcaaag cgctcccgaa atcctgcata cgcacggttt ccaggcaacg gcggatggtt
     4981 ccccggtctt tcacctttat tagcctgcgg tagcagttga gcatctcggt aacggttatg
     5041 gggaagccgg cgttcaaatc atcggctttt tgaggtacat agccggtttt ttttgcctga
     5101 ttttcaatcg ttccgccgac cggatgcaaa agattcagaa tcagtttaat cagtgtgctc
     5161 tttccactgc cgttttcgcc gagaatcgag agatattccc cgttcttgat ttcgagactg
     5221 atgttccgca agagaaacgg ttccgtgccg gtataggaat aataaaggtt tttcagatga
     5281 atcatgggtt cctccgcatt ctgaatggtg atggtttcga atcccggcga ttccaatttc
     5341 ttactgaatt tcaaaaccgc cggtgaaaca ttgacaagaa agtattacag gtttattata
     5401 attgcaaatg ctaaccgttt gcaatatgtt ttgggagatt ttatggatga ttcgaaaatt
     5461 tttcgccttt tttttgatta ttggcctgtt tctgactctt tcagcctgtt ccgaacccgt
     5521 aaagaagaaa agcggaggca aagtcggggt cgttgtcacc ttcaacgccc tggcggaatt
     5581 tgcccgcgcc gtcggaggca accgtatcag ccttacgaca attattccgg acggggtgga
     5641 gcctcacgat tttgagccga aggtcaaaga tatgaaatta atcagcgacg cggacgtt
//
LOCUS       DATALY010000251         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_399016,
            whole genome shotgun sequence.
ACCESSION   DATALY010000251 DATALY010000000
VERSION     DATALY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399016"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VHR42_06920"
     CDS             complement(<1..1105)
                     /locus_tag="VHR42_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026942.1"
                     /translation="MSKSVRILSLLTCLFLMVSLVAGCGTGTAQSTAATQSSAAAPAA
                     ASTEPQSSIAAAGQPLHKLSDTPVTFTIWKPGTPAIGKVIQSYEDSAAFQEMAKISNI
                     TLKFDTPVFGQEADKFNIMISSGEYPDLIEGIASYYSGGGDKAISDGVILKLNDYIAK
                     DAPDYTAARKANKTIEKETVTDTGNMYQFEEIVAGTTVEPPAWGLAIRQDWLDDLGLK
                     APVTYTDLHDVLKAFKDKKGATKPLSLDASGVPGFSSFTGGLGFYVALPGGICATSEY
                     FYQVDGKVKYGPIEPGYKEYLTMMAKWYKEGLVDPNFLSVKNFNDLISVMSSGKAGVA
                     VLPKPMFAALKASSSDPKFRVVALANPVKNPGDK"
     gene            complement(1320..>2139)
                     /locus_tag="VHR42_06925"
     CDS             complement(1320..>2139)
                     /locus_tag="VHR42_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3026943.1"
                     /translation="FDVSLSAALSNLHRGLKSIREAYREASDVFEYKQLKSEIMPVMR
                     YGENGFPPHSELKLSSFLVPERQFYDLVVDGNYKGAYNILKSIIGNCAGMDITSLHMV
                     KFRMFGLVNMMLNVLGEIKTDSNVKFFEKMDPINRLMNANTLAELQKETLCILDGIVQ
                     FFEKQDSNIKDKMEAVTGYINRQYYKTDLCVQFLSEKFGMSVSYLSRMYKKATGDGLL
                     DYIHRVRVEKAKVLLAGGMSIKDVAEKVGYYNSQALIRAFKRMEGITPGQYKVD"
BASE COUNT          506 a          539 c          497 g          597 t
ORIGIN      
        1 ttttatctcc ggggttcttt acagggttgg ccagcgcaac cacgcggaac ttcgggtcac
       61 tgctgctagc tttaagcgcg gcaaacatag gcttcggcag aacagcaacg cctgccttgc
      121 cgctggacat gacggatatc aggtcgttaa agttcttcac ggagaggaaa ttgggatcaa
      181 caagtccctc tttataccat tttgccatca tagtcagata ttctttatat ccgggttcaa
      241 ttgggccgta cttgaccttt ccgtcaacct gataaaaata ttcgctggtt gcgcagatgc
      301 cgccgggcaa cgcgacgtaa aaaccaaggc cgccggtaaa tgaactgaag cccgggaccc
      361 cggaagcgtc gagacttaac ggcttggtgg cgcccttctt atctttgaaa gccttcagca
      421 catcatgcag atcagtgtat gtcacgggtg ccttcaggcc caggtcatcc agccaatcct
      481 ggcgtatggc gaggccccaa gccgggggtt ccacagtagt accggccacg atctcttcaa
      541 actggtacat gtttcccgtg tccgtaactg tttccttttc aatcgtcttg ttggctttcc
      601 tcgcggccgt gtagtcgggc gcgtccttgg caatataatc gttaagcttt agtatgacgc
      661 cgtcactgat tgccttgtct ccgccgccgc tgtaatatga ggcgatgccc tcaatcagat
      721 ccggatactc gccggagctg atcataatat tgaacttgtc ggcttcctgt ccaaaaacag
      781 gggtatcgaa ctttaatgta atattggata tcttcgccat ctcctggaaa gcggcgctgt
      841 cttcatagga ttggataacc ttgccgatag ccggcgtccc gggcttccat attgtaaaag
      901 tgacgggagt atctgacaac ttgtgaagag gttggccggc ggctgcaatg ctgctctggg
      961 gctcagtgga agcagcggcc ggggctgcgg cgcttgattg cgttgcggca gtgctttggg
     1021 cggtgccggt tccacaaccc gccacgaggc tgaccattaa aaataagcat gttaacaacg
     1081 acagtattcg tactgacttt gacatctgta aattcctcct tcttaaatta aaaagaacaa
     1141 gagtacaggt taccgtaaat tcacccgacg cgtctttcgg taaattacat ccttagaata
     1201 ggtgtataac ggattaaagt aaatcggctg tttttatcct gcctataaac attttttgta
     1261 catccgctgc aatttctttt ccgttgaatc atcatttttt gaccggggat agtcattctt
     1321 taatccactt tatattgacc gggagtaata ccttccattc gcttgaaggc gcgaatcagc
     1381 gcctggctat tgtaataccc caccttctcg gcaacgtctt tgatgctcat cccaccggcc
     1441 agcagtacct tcgccttttc gacgcgaacc cgatggatat agtccagcaa gccatctcct
     1501 gtggcttttt tatacattcg tgacaaatag gaaacggaca tgccgaactt ctccgacaga
     1561 aattgaacgc ataagtcggt tttataatat tggcggttga tatagccggt gaccgcctcc
     1621 attttgtctt taatattgga atcctgtttt tcaaagaatt gcacgatgcc atccagaatg
     1681 cacaatgtct ccttctgaag ctctgcaagc gtattcgcgt tcatcaggcg attgatgggg
     1741 tccatctttt cgaagaattt cacattgcta tccgttttta tttctccaag cacattgagc
     1801 atcatgttca caaggccgaa catgcggaat ttgaccatgt gaagcgatgt tatgtccatc
     1861 ccggcgcaat tgccgataat gctttttaag atattgtacg cgcctttata attgccgtcg
     1921 accaccagat cataaaattg cctttccggc acaagaaacg agctgagttt caactctgag
     1981 tgaggcggga acccgttctc tccgtatcgc atgacaggca tgatttcgct cttgagttgt
     2041 ttatactcaa atacgtccga agcttcccgg tatgcttccc taatgctctt aagacctctg
     2101 tggagattgc tcaaagctgc tgaaagcgaa acgtcgaaa
//
LOCUS       DATALY010000252         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_486341,
            whole genome shotgun sequence.
ACCESSION   DATALY010000252 DATALY010000000
VERSION     DATALY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486341"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VHR42_06930"
     CDS             <1..666
                     /locus_tag="VHR42_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026944.1"
                     /translation="QDSLSALNPVYTIGIKLMETIRLHYSMTKAEARNRALELLKAVG
                     INEPENCMRCYPHQLSGGMRQRVLIAMAVSSSPKLLIADEPTTALDVTIQIQILRLLR
                     DIQARTGMSIIFITHDLGVVAQICSRIAVMCGGHIVEEGTAEEIFYQPFHPYTRALID
                     SITKKGADFTPYLERSSEQKEEDGLCPFLKRCGCCTESCKSALPDRYRMSDSHSLLCW
                     KRG"
     gene            671..1576
                     /locus_tag="VHR42_06935"
     CDS             671..1576
                     /locus_tag="VHR42_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3026945.1"
                     /translation="METNLIEVQNLEMYFQQKSAKTFSFRKNMLKAVDNVSFQIAKGE
                     TFGLVGESGSGKSTVGKCILRNYDLTGGKILFDGTDIGAMKEKKLLPFRRKMQSIFQD
                     PYSSLDPTKTIGAILREPMEIHRMFSKSEQKDRALELVNLVGLKKEDMEKYPAEFSGG
                     QRQRIAIARALTINPEFVLCDEPVSALDVSLQAQIVALLQQMQAKMGLTYLFISHQLH
                     LVQKICSHIGVMYLGNMLEITSADQLFSHPLHPYTQMLMSSILEPDPHINCLDRNIPD
                     MGLHARPRQGCKILQPLPKGEELLP"
BASE COUNT          577 a          493 c          520 g          513 t
ORIGIN      
        1 caggactctc tttctgcgct gaatcccgtt tacaccattg ggattaaact aatggaaacg
       61 attcgcctgc attattcgat gacgaaggca gaggcccgca atcgggcgct ggaactgctg
      121 aaagcagtcg ggatcaacga gccggaaaac tgcatgcgct gttacccgca ccaactcagc
      181 ggcggtatgc gccagcgcgt actgatcgcc atggccgtca gcagcagtcc aaagcttttg
      241 attgccgacg aaccgacaac cgctcttgac gtgaccattc aaattcagat tctccgcctt
      301 ttacgggata ttcaggcccg aaccggaatg agcattatct ttattacgca cgatttgggc
      361 gtggtagccc aaatctgctc cagaatcgct gttatgtgcg gcggccatat tgtggaagag
      421 ggaacagcgg aagaaatctt ttatcagcct ttccatccgt acaccagggc actaattgac
      481 agcattacaa aaaaaggtgc tgattttacg ccctacctgg agcgcagttc cgaacaaaag
      541 gaagaggacg gactttgtcc ttttctgaag cggtgtgggt gctgtacgga atcctgcaaa
      601 tcagcccttc ctgatcgtta cagaatgagc gattcccaca gtttgctttg ttggaaacgg
      661 ggataataac atggaaacaa acttaattga agtgcagaat cttgagatgt acttccagca
      721 aaaatcagca aagacattct cctttcggaa aaatatgctg aaagctgtgg acaatgtgag
      781 ctttcagatc gcgaaaggag aaacattcgg gctggttggc gaatccggga gtggtaagtc
      841 aacggtaggg aagtgcatcc ttcggaatta tgacctgacc ggaggcaaaa tcctgtttga
      901 tggaaccgat attggggcaa tgaaagaaaa aaagcttctt cctttccgac gcaagatgca
      961 gtcgatcttc caggaccctt attcttctct ggacccgaca aaaaccattg gggctatttt
     1021 acgggaaccc atggaaattc accgcatgtt ttcaaaatcg gaacaaaaag accgtgctct
     1081 ggaactggtg aatctggtcg gattaaaaaa agaagatatg gaaaaatatc cggcggagtt
     1141 cagcggcggg cagcgtcagc gaatcgccat cgccagagcg cttacaatca atccggaatt
     1201 tgttctgtgc gatgaaccgg tctctgctct ggatgtttct ttacaggctc aaattgttgc
     1261 tcttctgcag caaatgcagg caaagatggg attaacgtat ctttttatca gccatcagct
     1321 ccatctggtt cagaaaatct gcagccacat tggggtcatg tatttaggga acatgctgga
     1381 gatcacttct gcggatcagc tcttttccca tcctttgcat ccctatacgc agatgctgat
     1441 gtcatcgatt ctcgaaccgg accctcatat caactgttta gatcggaata tccctgacat
     1501 ggggctgcat gccagacccc ggcagggatg caaaatattg cagccgctgc ccaaaggcga
     1561 agagctgctg ccatgaaacc gcacctgccc tgcgtgaaat tgaacctggc cattttgtag
     1621 cctgctatct gtatgaatag cggaaaataa gacggagttt aatttgatgg gatcataccg
     1681 tgtcgggaat gcaactctcc gcaaaaaatg caactatttc agccggatgc aaccctttta
     1741 aaggaatcgt gtaattgtat caaactttag tttactaggc aaaagaccta tacggaagat
     1801 ttcctgacca agaaaataaa agtgaataac ggtgaaatcc aaatgtacta catagaaata
     1861 gccattctgc gattgtacct cccgagacct atgaactggt tcaggaggaa tttcgccgca
     1921 gaaaggctgc ggaatcgtac cagcgtaatt aactgtttcg cgagccgcat agtttgtgga
     1981 gactgcggtg gattttaccg cctgtgacag tgagcctatt tgccccggga atggaaacgt
     2041 tgccgttaat acccgggctg ccggacttat gccagccaat gtcactggtg ccggtgaggg
     2101 aac
//
LOCUS       DATALY010000253         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_399528,
            whole genome shotgun sequence.
ACCESSION   DATALY010000253 DATALY010000000
VERSION     DATALY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399528"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VHR42_06940"
     CDS             <1..442
                     /locus_tag="VHR42_06940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3026946.1"
                     /translation="RECVDHPGGVTVAVLTKEDEMLFVRQFRYPYREVVLEAPAGKLE
                     KDEDPFEAMKREQQEETGTTGTGYIDLGKLYPSPGYCNEIIYLYACRAESFGKTNFDE
                     DEFLEMERIPMNEAVEMVMSNEIVDAKTQVLVLKTAQLLKDSRI"
     gene            455..739
                     /locus_tag="VHR42_06945"
     CDS             455..739
                     /locus_tag="VHR42_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026947.1"
                     /translation="MKKQRKKIEMDERQKRLLLYVSLFIGAVFFAIWDPTQLWLVIGL
                     GIGIVYGLAHDWIFTRDKKKNEPEPPTEMSMEEPESVEKIEEADRSEKDK"
     gene            complement(907..2070)
                     /locus_tag="VHR42_06950"
     CDS             complement(907..2070)
                     /locus_tag="VHR42_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX3026948.1"
                     /translation="MAELCPFRALRCTDRASIPIQAETGENPALDRKLADWLSNQKKG
                     DETLKQDTDPGLMIYEQEFTHLGQRKKVKGLLSLVKIEEETILLPHEEVSIQKEQEHF
                     ELLQKTGCQLRPVYCMYEDDERKTMSRVQLLSSGKPRFEWKENGTVHRIWLVNDILVI
                     AAIKEDFLDRRLFLADGHSRYAAAQAIRQSGGSDYIFTFLADMEQEFTFLPSHCLIRN
                     LESWNEAKFLADCEPFFQVIPRGTVDEIPTNLDALYRQGKKAFAFYSGGANWTLLILK
                     DTELMGELLPDKSESFRQLDVSIFHSLVLEKLLAIPKDNENAISTDSIEEALTSVQNG
                     EAQCAFFLNPARRKEICEISEVGEKMPPQSTSFFPVFPVAAAIYKSTEIDFIK"
     gene            2388..>3302
                     /locus_tag="VHR42_06955"
     CDS             2388..>3302
                     /locus_tag="VHR42_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3026949.1"
                     /translation="MIEVKNLSKRYGQNVALNNISFSVEEGTVVGFLGPNGAGKSTTM
                     NIITGYLSASSGVVTVGGYNTLDNPNEVKKLIGYMPELPPLYPDMTVKEYLNFMYDLK
                     KVTLPRDKHLKEICALVRISEVYGRLIGNLSKGYKQRVGIAQALLGNPPVLILDEPTV
                     GLDPKQIIEIRNLIHNLGKKHTIILSSHILPEIQAVCERIIVINRGNLVADGTPDSLA
                     HNLSTDHKLVVRLAGAEAEIKAGLKTIHNVVGVESFGEKEPGVYEFTVEAREGTDLRR
                     DLFHFAAKKDWPILSLTNSDLSLEDVFLR"
BASE COUNT          927 a          750 c          780 g          845 t
ORIGIN      
        1 cagggaatgt gttgaccacc cgggcggagt caccgttgca gttttgacaa aagaagacga
       61 aatgcttttt gtccgccaat tccgttatcc ttaccgggaa gtggttctgg aagcgcccgc
      121 agggaaactg gaaaaagacg aagatccgtt tgaagcgatg aagcgcgagc agcaggagga
      181 aaccggcaca acgggtaccg gttatattga tttgggcaaa ctgtacccct cccccggtta
      241 ctgcaacgag attatttatc tttacgcatg ccgtgccgaa tcgttcggta aaaccaattt
      301 tgacgaagat gagttcttgg aaatggaacg tatcccgatg aatgaagctg ttgaaatggt
      361 aatgagcaat gagattgtcg atgccaaaac acaggtgctg gttttaaaaa ccgcacagct
      421 tttaaaggac agcagaattt aagccggagg atatatgaaa aaacaaagaa aaaaaatcga
      481 gatggatgaa cggcaaaaac ggctgctgct gtatgtcagc cttttcattg gtgctgtctt
      541 tttcgcaatt tgggatccaa cccagctgtg gcttgtcatc ggtttgggta ttggtatcgt
      601 gtacggtcta gcgcacgatt ggattttcac ccgagacaag aaaaagaacg aaccggaacc
      661 accgactgag atgtcaatgg aagagccgga atcggtggag aagatagagg aagccgatag
      721 gtcggaaaaa gacaagtaaa acgaatgcgg accaaacttt ccacaaaaag tgaaaacatt
      781 gtggaaaaca aaattaaatt tcaaataatc gccgccgcct tcggcggaat atagtaagtc
      841 cccgacaggt ttaccctgtc ggggactttt tcgtctgtat cctcataaac ccagatggaa
      901 tgaaaatcat tttataaagt caatttcggt tgacttgtat attgctgccg caacaggaaa
      961 aacgggaaag aaagaagtcg actgtggggg catcttctcc cccacttctg aaatttcgca
     1021 aatctctttc cgacgtgccg ggttcagaaa gaacgcacat tgtgcttctc cattttgaac
     1081 agacgtaaga gcttcttcta tggaatctgt agaaatcgcg ttttcgttat cttttggaat
     1141 cgcgagcagc ttttccaata caaggctgtg gaaaatagat acatcaagct gacggaacga
     1201 ttcgcttttg tcgggcaaaa gttcccccat caattcggtg tccttcaaaa tcagcaaagt
     1261 ccagtttgca ccgccggaat aaaacgcaaa agcctttttt ccctgccggt aaagtgcatc
     1321 cagattcgta ggaatttcat ctacagtccc ccttggaatc acttgaaaaa aaggttcaca
     1381 gtctgcaaga aattttgctt cgttccagct ttctaaattc cgaatcaggc agtgggacgg
     1441 caaaaaggta aattcctgtt ccatatctgc caaaaaggtg aagatataat ccgaaccacc
     1501 actttgtcgg atagcctgcg ctgcggcgta tcgggaatgg ccgtcggcaa gaaaaagccg
     1561 acggtcaaga aaatcctctt ttatggcggc aatgaccaga atatcgttca ccagccagat
     1621 tcggtgtacc gttccgttct ctttccattc aaagcgcggc tttccgcttg aaagcagttg
     1681 cacgcggctc attgttttgc gttcgtcatc ctcgtacata cagtagactg gccgaagctg
     1741 acagccggtt ttttgcagta gttcgaaatg ctcctgctcc ttttgaatgg aaacttcctc
     1801 atgtggaaga agaatcgttt cctcttcgat tttaacaaga gaaagaagac cttttacctt
     1861 ctttctttgg cccaagtggg tgaactcctg ttcatatatc atcaagccag ggtcagtatc
     1921 ctgcttcagg gtttcatccc cttttttttg attgctcagc cagtccgcca gcttccggtc
     1981 cagagcagga ttttcgcccg tttctgcttg aatcgggata cttgcccggt cggtgcagcg
     2041 cagtgcgcgg aaaggacata attctgccat attcaacact ccaacagtaa ttttcaagta
     2101 tcttaattgt accggtcggc gtcgattccg tcaagcctgc gaaacttcac ctaaccatca
     2161 cctaaaatta aaaaaatagc ttgttaatat taaaagaaag tttacaattc accatagata
     2221 aattgctaaa atagaagaga tttaaaagca gccatgctct ttttttatgc aatttccttt
     2281 ttgcagatga tattaagatt ttttcttcat cgtaataaag aaagggaacg acaaaagcgg
     2341 taagatggcg taggtaagag cgggcatgaa aacaggaggg actcaggatg attgaggtca
     2401 aaaacctctc aaagcgttac ggtcaaaacg tcgctttgaa caatatcagc ttctcggttg
     2461 aggagggcac tgtcgtcggc tttttaggcc cgaacggcgc cggtaaatca accaccatga
     2521 acatcatcac cggctatctg tcggcaagct cgggagtagt gaccgttggc ggttacaata
     2581 ctcttgataa tccgaacgag gtgaaaaagc taatcggcta tatgccggaa ctgcctccgc
     2641 tttatcccga tatgaccgta aaggaatacc ttaactttat gtacgatttg aaaaaggtta
     2701 ctcttccgcg ggacaaacat cttaaagaaa tttgcgcgct ggttcgtatc tccgaggttt
     2761 acggccgatt gattggcaac ctttccaaag gttacaaaca acgtgtaggt attgcacagg
     2821 cacttttggg caatcctccc gttttaattc tggacgaacc caccgtaggc cttgacccga
     2881 agcagattat cgagatccgc aacctgattc acaacctcgg taaaaagcat acgattattt
     2941 taagctctca tattctgccg gaaattcaag cggtgtgcga gcggattatt gtcattaacc
     3001 gcggcaacct tgtggccgac ggaacgccag acagtctggc tcataacctg agcaccgacc
     3061 acaagctggt ggtgcgtctt gccggtgcgg aagcggaaat caaagccggg ctgaaaacca
     3121 ttcacaatgt tgtgggtgtg gaaagcttcg gcgaaaaaga accgggcgtt tatgagttca
     3181 cggtcgaagc gcgcgaaggc accgacctgc gccgtgacct gttccatttt gcagcaaaga
     3241 aggactggcc gattctgtcg cttaccaatt ctgacctttc gctggaagat gtgttcctgc
     3301 gt
//
LOCUS       DATALY010000254         6237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_660330,
            whole genome shotgun sequence.
ACCESSION   DATALY010000254 DATALY010000000
VERSION     DATALY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660330"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..251
                     /locus_tag="VHR42_06960"
                     /pseudo
     CDS             1..251
                     /locus_tag="VHR42_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(783..2786)
                     /locus_tag="VHR42_06965"
     CDS             complement(783..2786)
                     /locus_tag="VHR42_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026950.1"
                     /translation="MKKQTLLTKILVCVGIPVVVIYLLFSVYLLQAVKQSVTELTTKD
                     LVSKSRAASYQVNNYFIKYVGIAQQLMLNSTVQNFVGSTSPKKKIQDTPGFKDVDRTL
                     RNISATDQSNIIDSWVADTATNHCIDQTTGVSDYDLSSRPWYQQLAKGRQIVITEPYE
                     DVDTKALTVSMIAPIFDGNTLIGAAGIDITMNHLYDTIKSYKLGDTGFFILTSAGGKL
                     VYYPDAAMKNKSVAQSKMSANIVEAIQNKKEGSLSYTAMGGTNYGYLSPIGTTGWTIT
                     TGLPEGEFYAPFNQIFLSLMVAFLIAAAVIFMTILFTSKSIVRPLVKLKNSANEIADG
                     DLNITVDVHSADEIGQVATALSRTVDRLKQYINYIDEVSAVLDQIAVGNLMFELHCDY
                     VGEFSKIKASLENIKSSMKGLVEEIGVSADQVANGSGQVSNSAQSLAQGATEQAGSLE
                     ELSASIEEVSNHVKNNTVQISHIAVSMNSAAQDVEESSGRMDQMLAAMNEISASSNEI
                     GNIIKVIDGIAFQTNILALNAAVEAARAGEAGKGFSVVADEVRSLAGKSADAAKQTSE
                     LIGNSVKKVEEGLALADGTAKVLTGMAGQVESINETIQQVKDASGQQFLAIKQVTQGV
                     EQISAVVQTTSATAEESAAASEELNGQAQSLKELVSHFKVESN"
     gene            complement(3066..4154)
                     /gene="trpS"
                     /locus_tag="VHR42_06970"
     CDS             complement(3066..4154)
                     /gene="trpS"
                     /locus_tag="VHR42_06970"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX3026951.1"
                     /translation="MENIILTADRPTGKLHIGHYVGSLRSRLALQEAGGYSRMYVMIA
                     DAQALTDNADNPEKVRENLIEVALDYMAVGLDPKKITFFIQSMVPELTELTFYYSNLV
                     TLARLERNPTIKSEVQLRGFQSNIPVGFLTYPISQTADITAFKANIVPVGEDQKPMLE
                     QAREIVRKFNSTYGETLIEPQEMLPQNEAMCRLPGTDGKNKMSKSLGNCIYLCDDEKT
                     IKTKVMSMYTDPNHLRVEDPGNVENNPVFLYLDAFCTDAHFAEYLPDYKNLDELKAHY
                     KHGGLGDVKIKKFLNSVLQEELLPIRQRREEYSHKIPEIFEILKRGSEAARESAAKTL
                     SDVKHAMRIDYFEDAGFVESQAREHGAK"
     gene            4520..4993
                     /locus_tag="VHR42_06975"
     CDS             4520..4993
                     /locus_tag="VHR42_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3026952.1"
                     /translation="MDSNILNIEQATEFLGVSEKTLIKLLREEHLPARKIGREWRFSR
                     SALIDWLAGGDSIRYANQNEPYIVSKDIPGKTAALFKKINESLADVKNSGNNIKTLLP
                     DLNDDTVIPKNAALRVSYKRQRDVEKLEFKIFWTQPDDIKSETDREAAPAEPEEG"
     gene            4995..5855
                     /locus_tag="VHR42_06980"
     CDS             4995..5855
                     /locus_tag="VHR42_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3026953.1"
                     /translation="MWVLYACGSAFFAGITSILAKVGIRKTDSDLATAIRTVIVLLFS
                     WLMVFVVGSQNTIGAISLQSLVFLILSGLATGASWLCYFKALQLGTVNRVTPVDKSST
                     ILTIILAFLFLHETPKPLSVIGTILLAAGIYLMIQRRDSEQEDVKSGSWLFYAVLSAV
                     FAALTSILGKVGIQGIESNLGTAIRTAVVLVMAWGIVLARGKQTEIKKIDGKSWAFLG
                     LSGLATGLSWLCYYHALQEGKASVVAPIDKLSVVVTIVFSRVFLGEKLTKKAFVGLLL
                     LVAGTALMLF"
     gene            complement(5852..>6237)
                     /locus_tag="VHR42_06985"
     CDS             complement(5852..>6237)
                     /locus_tag="VHR42_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEX3026954.1"
                     /translation="DFLETNDGWVIDGNYRRFLQKERLEQADQIVILSFPRLLCFYRA
                     WKRFFRNKNRTRESMATGCAEKMDREFMRWILWEGRTKKRWQSYQEIAARYPEKTILL
                     KNPRQVAQFVNRTITEESPEAFRAD"
BASE COUNT         1508 a         1532 c         1456 g         1741 t
ORIGIN      
        1 ttacacaaga aagagtttct gaagaaatta aggtgtccag acaaacaata tcaaattggg
       61 aaaatgaaaa atcgtatcct gacatcatca gtgtcatcaa actgagtgac ttttacaacg
      121 ttagcttaga tgagctgctc aagaatgatg atgaaatgat aaagtatcta gataaaagca
      181 caaataccgt gaaaagcaac agaagactaa gcattgtcgc tgtaattagt ctaacagtaa
      241 tcgtgacact tatgatcctt ttaggcgacc gcagtaatat attgatttgg gtaacggtgc
      301 ttttagagtt tgttctactt tgtattgtgg acttttctga tgcaccggga gttagtaaac
      361 tgaccaaaat tgtgagccgg gttctgatgg ccttttgcgt tattttatat ttgggaatat
      421 tggtcatttg tggtttggcg gcttttgatt cctttaaatc aggacaaata gaagttggca
      481 ttaaattatt gattacgatc attgttgttg ctgtttgcgt atatgtttta ttaattcgta
      541 aatttataaa gatactatca aaaaaagatt aaagaaataa agagacgccc gatgcgcgct
      601 cttttgatgc ccttttgaaa accttaccgg tcgcttttga tgcgattacc gttcaagata
      661 tccttgggta gtttcatttt gacggttatt tctgcttata agttaaattg ctataagtaa
      721 aaacaagcaa acagaaaaat tcaggccggc cttttcgggg ccggcctttg ttggaatatc
      781 aatcagtttg attcaacttt gaaatgtgag acaagttctt tcagcgactg ggcctgacca
      841 ttcaattcct cgcttgcggc agcgctttcc tccgccgtag ccgaagtggt ctgcacaacc
      901 gctgaaattt gttccacccc ctgcgtaacc tgtttaatgg caaggaactg ctggccggaa
      961 gcgtccttaa cctgctggat ggtttcgttg atggattcca cctgcccggc cattcctgtt
     1021 aagaccttcg ccgttccgtc tgcgagtgca agaccttctt cgactttttt caccgagttg
     1081 ccgatcagct cggatgtctg ttttgccgca tctgccgatt tgcccgcaag gctgcgcacc
     1141 tcgtcggcga caaccgagaa gccttttccg gcttctcccg ctcttgccgc ttccaccgcg
     1201 gcgttcaggg caaggatatt cgtctggaaa gcgatgccgt caatcacctt gatgatgttt
     1261 ccaatttcat tcgacgaagc ggaaatctcg ttcatcgcag ccagcatctg atccatgcgc
     1321 ccgctgctct cttcaacatc ctgcgccgca gaattcatgc tgacggctat gtgactgatc
     1381 tgaaccgtgt tattcttaac atgattggaa acttcctcaa tggacgccga gagctcttcg
     1441 agggagccgg cctgttcggt ggcaccctgc gcaaggcttt gcgcggaatt cgacacttga
     1501 ccggaaccgt ttgcgacctg atctgcggaa acgccgatct cttcaacaag tcctttcata
     1561 gaagacttga tattttccaa agaggccttg atttttgaaa attccccaac atagtcgcag
     1621 tgcagctcaa acatcaggtt gcctactgcg atctggtcga gaacggcgga aacctcgtca
     1681 atataattaa tatactgctt caaacggtca accgtgcggg aaagagcagt ggcaacctgg
     1741 ccaatttcat ccgcactatg cacgtcaacg gtgatattga ggtctccatc cgcaatttca
     1801 tttgccgagt ttttcagctt caccagaggc cgcacaatgc ttttcgacgt aaacagaatc
     1861 gtcatgaaaa tcacggcggc ggcaatcagg aacgccacca tcagtgacaa aaagatctga
     1921 ttgaagggag cataaaattc gccttccggc aagccggtcg tgatcgtcca gccggttgtt
     1981 ccaattgggg aaaggtagcc gtaattcgtg ccgcccattg cggtatagct gagcgagccc
     2041 tcttttttgt tctgaatggc ttcgacgatg tttgcggaca ttttggattg cgcgacactt
     2101 ttgtttttca tcgcagcatc cgggtagtaa acgagttttc cgcccgcact agtcagaata
     2161 aagaagccgg tgtcccccag cttatagctt ttaatcgtgt catagagatg gttcatcgtg
     2221 atgtcgatgc ccgcggctcc tattaatgta ttcccgtcga aaatcggcgc aatcatgcta
     2281 accgtcaacg ctttggtgtc aacatcctca taaggctccg taatgacaat ctgcctgcct
     2341 tttgcaagct gctgatacca tggccgagag gaaaggtcat agtccgagac gccagtcgtc
     2401 tggtcgatgc agtgatttgt ggctgtgtcc gccacccagg aatcgataat atttgactga
     2461 tcggtcgccg aaatattccg cagcgtccgg tcgacatctt taaaaccggg agtatcctga
     2521 attttcttct tgggtgaagt gcttcctaca aaattctgca cggttgagtt gagcatcagc
     2581 tgctgtgcaa ttcccacata ttttataaaa tagttattga cctgataaga agccgcgcgc
     2641 gatttggaaa ctaaatcctt cgtcgtgagt tccgtaaccg actgcttaac cgcctgcaat
     2701 agataaacgg aaaaaagcag ataaattact acaacaggaa ttccaacgca tacaagaatc
     2761 tttgttaaca gggtttgctt tttcatttgc tgagatcaca ccttatttca tataatgatt
     2821 aaattctatc aagttaattc ttttgcataa taacagtcca gaattattaa aaaattatga
     2881 atgccaaatt ctaaaaaaga acggagtggt gccgaaaaac tgggcatttt cccctccgca
     2941 aatttaaagc ggcacgcggg ggacagatac acaccgcgac aaggctgtaa aattttcgcc
     3001 acgccaaaag tgcagcaaaa aagggcagct tttcagccac cctttttcga gttcaaatct
     3061 ccgttttatt tcgcgccgtg ttcccgtgcc tgactctcga caaaaccggc atcctcaaag
     3121 tagtcgatcc tcatggcgtg cttcacgtca ctcagcgtct tcgcggcgct ttctctcgcg
     3181 gcttcactgc cgcgtttcag gatctcaaag atttcaggga ttttatgcga atactcttcc
     3241 ctgcgctgcc ggatgggaag aagctcctcc tgcagaacgc tgttgagaaa tttcttgatc
     3301 ttcacgtcgc cgagaccgcc gtgtttgtag tgtgccttca gttcgtctag atttttgtaa
     3361 tccggcagat attccgcaaa atgcgcgtcc gtgcagaaag catcaagata gaggaacacc
     3421 gggttgtttt ccacattgcc ggggtcctcg acccttaagt gattcgggtc ggtatacatg
     3481 ctcatgacct tggttttgat ggtcttttca tcatcgcaaa gataaatgca gttgccgagt
     3541 gacttgctca ttttgttctt gccgtccgtt cccggcagac ggcacattgc ttcgttctgc
     3601 ggcagcatct cctgcggctc aatcagcgtt tcgccgtaag tgctgttgaa tttacgcaca
     3661 atttcgcggg cctgttccag catcggcttc tggtcttccc caacgggaac gatgttggct
     3721 ttgaacgccg tgatgtccgc cgtctggctg atcgggtaag tcaggaagcc gacggggatg
     3781 tttgactgga aaccccttaa ctgaacttcg cttttgatgg tcgggttgcg ttccaacctc
     3841 gcgagcgtta ccagattgct gtaatagaac gtgagctccg tcagctccgg caccatggac
     3901 tgaatgaaaa aagtaatttt tttcgggtcc agaccaacgg ccatgtaatc gagtgcaacc
     3961 tcaatgaggt tttcgcgtac cttttcgggg ttgtcggcat tgtccgtcaa ggcctgcgcg
     4021 tccgcaatca taacgtacat tctgctgtag ccgccggctt cctgcagtgc cagcctgctt
     4081 ctcagggaac cgacgtaatg cccgatgtgc agttttccgg tgggcctgtc agcggtcaaa
     4141 atgatgttct ccatgggttt cataattcct ttcaaaattc acaatatgtt tcttttggaa
     4201 ttgttgtctt ttcaccgaga ccgtcccttt cagggattcc cggcttttcg tgtttctatt
     4261 ttatcacgtt ttcggaatga cagcctatcc catcttgtaa aaatgtaatt ctttttcgtt
     4321 ttttcattca ccctgccgct cgctgttcgt tttccagaaa tcagcggcgg aattgctgtt
     4381 tggaagataa aaggagttaa ttccctttat tataatatta atttgtccct atctgcttcc
     4441 attttgctgt tatatacagt tatgcttgac agagcagcca gacacgccta taatctcaca
     4501 tagaaaaggg gcattttgaa tggacagcaa tattttgaat attgagcaag ccacagaatt
     4561 tctcggggtc agtgaaaaga ccctcatcaa gcttttgcgc gaagaacatc ttcccgcgcg
     4621 caaaattggc cgggaatggc ggttcagccg cagcgccctc attgactggc ttgccggcgg
     4681 cgattccatc cggtacgcca accagaacga accatatatt gtctcaaagg acataccggg
     4741 taaaaccgcc gcactattca aaaaaattaa tgaatctctc gccgatgtga aaaacagcgg
     4801 caacaacatt aagacgctgc ttcccgatct caacgacgac accgtgattc cgaaaaacgc
     4861 agcgcttcgc gtcagttaca aacgtcagcg ggacgtggaa aagctggaat tcaagatttt
     4921 ctggactcag ccagacgaca taaagtcgga aacggaccgt gaagcggcgc ctgccgaacc
     4981 ggaggaaggc tgaaatgtgg gttttgtacg catgcggctc ggcctttttc gctggcatca
     5041 cgagcattct cgccaaagtg ggaatcagaa aaacagactc cgatttggca acggcaatcc
     5101 gcacggttat cgttcttctt ttttcatggc tgatggtctt cgtcgtcggc tcgcagaaca
     5161 caattggtgc tatttcgttg caaagccttg tttttttgat tctctcaggg cttgccacgg
     5221 gtgcgtcgtg gctgtgttat ttcaaagctt tacagcttgg aaccgtcaac cgagtgacgc
     5281 cggttgacaa aagcagcaca attttgacga tcatccttgc tttccttttt ttgcatgaaa
     5341 ccccgaaacc tctttccgtc atcgggacga ttttgcttgc cgcaggaatc tacctcatga
     5401 ttcaaaggcg cgattcggaa caggaagacg taaaaagcgg ctcatggctt ttttacgcag
     5461 tcctgtcggc tgtgttcgcc gccctgacct ccattctcgg caaagtcggg attcagggaa
     5521 tcgaatcaaa cctcgggacg gcaatccgca ctgccgtcgt tctcgtcatg gcatggggaa
     5581 tcgtgctggc acggggaaag caaaccgaga tcaagaaaat tgacgggaag agttgggcat
     5641 ttctcgggct ttccggcctt gcgacggggc tttcgtggct ctgctattac cacgctctgc
     5701 aagaaggcaa agccagcgtt gtcgcaccca tcgacaagct gagcgttgtg gtaacaatcg
     5761 tgttttcccg tgtcttcctg ggggaaaaac tgaccaaaaa agcgttcgtg ggcctgcttc
     5821 ttcttgtcgc tggcaccgcg ctgatgctct tttaatccgc ccgaaaagct tcggggcttt
     5881 cctctgtgat ggttcgattg acaaattgag caacctgacg tggatttttt aacaggatgg
     5941 ttttctccgg ataacgggca gcaatctctt gataggattg ccaccttttt ttcgttcttc
     6001 cctcccaaag aatccaccgc atgaattccc gatccatttt ttcagcgcag ccggtcgcca
     6061 tactttcacg cgttctattc ttgttgcgga aaaaacgttt ccatgcgcgg tagaaacaga
     6121 gcagacgggg aaaactcagg atgacgattt ggtccgcctg ctccagccgc tccttctgca
     6181 aaaatctgcg gtaattgccg tcaatcaccc atccgtcgtt tgtctccaga aaatctg
//
LOCUS       DATALY010000255         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_55377,
            whole genome shotgun sequence.
ACCESSION   DATALY010000255 DATALY010000000
VERSION     DATALY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55377"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VHR42_06990"
     CDS             <1..213
                     /locus_tag="VHR42_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026955.1"
                     /translation="RYFRPSSFRSLTVLNTRVKMPTSIGITVTRIAQSGTPAMLRSKT
                     AERRKPEVTSTKISRTLCESRVRLFV"
     gene            complement(239..958)
                     /locus_tag="VHR42_06995"
     CDS             complement(239..958)
                     /locus_tag="VHR42_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3026956.1"
                     /translation="MPQYTTGELAKLCGVTVRTVQFYDGKDLLKPSDLTEGGRRLYTE
                     DDVGRLRMICMLKALGLTLGSIQGILGSDSPDKVLILLLEEQTKRLGDEIKDRRKQLE
                     TIRAIQESIRGAGRIPVNSISDVEHMMNSRKKLRKTHAVMLILGLVMDAIQIAALILW
                     IVKGIWQPFAIGMPIVILLGVLVTWMYCKNTDYICPECNVQFKPTLHDFLFSSHTPKT
                     RKLTCPKCGHTGYCVETASQK"
     gene            complement(1302..1676)
                     /locus_tag="VHR42_07000"
     CDS             complement(1302..1676)
                     /locus_tag="VHR42_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX3026957.1"
                     /translation="MKLAEKAARLKTDIPAVFLCMKSKETPAAAKVLAAITIGYALSP
                     VDLIPDFIPFLGYLDDLLILPLLVMLTIRLIPKETFERCRVQAQGMWKDGKPKKWFYA
                     IPVVIIWLVVLWLILRAFLLGK"
     gene            complement(1862..>2082)
                     /locus_tag="VHR42_07005"
     CDS             complement(1862..>2082)
                     /locus_tag="VHR42_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-enoate reductase"
                     /protein_id="HEX3026958.1"
                     /translation="FDGSILQVETAEGVQSVEADTVILAVGYTSENELYEQLRDEVPE
                     IHLLGDARRVANIMYAIWDAYEVASHIE"
BASE COUNT          493 a          558 c          481 g          550 t
ORIGIN      
        1 cgttatttcc ggccttcttc tttccgcagc cttacggtgc tgaacaccag agtaaaaatg
       61 ccgacaagca tcggaattac ggtcacacgg atagcccagt ccggaacgcc tgccatgttg
      121 cgcagcaaaa ctgcggaaag aagaaagccg gaagtaacca gcaccaaaat aagccgcacc
      181 ctgtgtgaaa gcagggtgcg gctttttgtg tagcgaaatt ttccggacat tggcttgttt
      241 acttttggga cgccgtctcg acgcagtaac cggtgtgccc gcatttcggg caagtcagtt
      301 tccgcgtctt cggcgtgtga ctcgaaaaca gaaaatcgtg cagggtcggc ttaaactgga
      361 cgttgcattc cgggcagata taatccgtgt ttttgcaata catccacgtt accagcaccc
      421 cgagcaaaat tacaatcggc attccgattg cgaaaggctg ccagattcct tttacaatcc
      481 aaaggatcag agccgcgatc tggattgcat ccatcacaag gccaaggatt agcatgactg
      541 cgtgtgtttt tctcaatttc tttctgctgt tcatcatgtg ctccacgtcg ctgatggaat
      601 taaccggaat tctgcctgcc ccgcgaatgc tttcctgaat tgcacggatt gtttccagct
      661 gcttccggcg gtctttgatt tcgtcgccga gtcgcttcgt ctgttcctcc agaagaagga
      721 tcagaacctt atcagggctg tcgctgccaa gaatcccctg aatggacccg agcgtaagtc
      781 caagagcttt cagcatacag atcatgcgca gcctgccgac gtcgtcttcc gtatacaggc
      841 gtcttccgcc ctccgtcaga tccgacggct tcagcagatc tttcccgtcg taaaactgca
      901 cagtgcgcac ggtaaccccg cagagttttg ccagttctcc ggtcgtatat tgcggcatag
      961 agctcacctc cttaccctca ctttacctca tgacgcagcg taacgagcaa gaggtgacgc
     1021 tgcgttattt ttaatttttt tgtttttcgg gctgattcgt acttatgatt ctcgttatgg
     1081 aatagatagt catctgtttc catggagatt atagcatttc tttttccttc cgtacggggg
     1141 cgggaaatta tcatcatgcc atttgccaca cggctgtttg cctcttttcc gctctgatat
     1201 taagcaaaaa cggaattggg attacgcccc ggagcccccg atatcagtgc agcgacaaag
     1261 ctgctaaaat cagaataaaa aacagagata acttcatact cttacttccc caacaaaaac
     1321 gctctgagaa tcagccacaa gacaaccagc cagatgatca caacgggaat ggcatagaac
     1381 catttctttg gctttccgtc cttccacatg ccctgtgcct gaaccctgca gcgctcaaaa
     1441 gtctcttttg gaatcagccg aatggtcagc atgaccagca aaggcagaat cagcaaatca
     1501 tcaaggtagc ctaaaaacgg aatgaaatcc ggaatcaggt caacgggtga cagtgcatag
     1561 ccgattgtaa tcgctgccag gacttttgcc gcagcgggtg tctctttgct tttcatgcag
     1621 agaaacactg ccgggatatc cgttttcagc cgtgcagctt tctctgcaag tttcatcgtt
     1681 tatgccccgc aatcctcatg tcccctgccg ccgggatgtt ccgtttgcat ttgaactcgt
     1741 ggcatgaaaa caccccattt gcttttcagt atatcatact gtctcacaaa tggggtgcag
     1801 ttcaagaaaa tgtgttttag ctgcggccgg ctccgggtgc ccgccggtag atttctacca
     1861 tttattcaat atggctggct acttcgtagg catcccagat tgcatacata atgttcgcta
     1921 ctctgcgggc gtcgcccagc agatggattt cgggaacttc gtcgcgaagc tgttcgtata
     1981 attcgttttc tgatgtgtag ccgacagcga gaatcacagt gtccgcctcg acagactgta
     2041 ccccttcggc tgtttccacc tgaaggatac taccatcaaa gc
//
LOCUS       DATALY010000256         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_486729,
            whole genome shotgun sequence.
ACCESSION   DATALY010000256 DATALY010000000
VERSION     DATALY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486729"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1052)
                     /locus_tag="VHR42_07010"
     CDS             complement(225..1052)
                     /locus_tag="VHR42_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3026959.1"
                     /translation="MKYSFLTSVYRRYSMEKAFEDGARFGYQGIELWGGRPHAYPFDM
                     DDKRIERVHRMARNTGLEISVYTPEVCVYPYNLCSQEEKERRETIEYFKLSLDIAKEI
                     GAPLIQITPGDPGYGSDYQKDWARLVEGCREIAEHAESVGVGIVMEALTPYESLILTT
                     TNDLLRLLGEVPSEYFRSMLDCVSPLVVHEPMSDYFIKLKNKMGHLHLADGDGISYSH
                     LLVGDGEFNFDGLFRLVNRYGYKGYGSIELDDRYCNDPETYIEQSITRLKRIQSRVE"
     gene            1149..1544
                     /locus_tag="VHR42_07015"
     CDS             1149..1544
                     /locus_tag="VHR42_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3026960.1"
                     /translation="MLMYFRNQKKSSQDVYISDPIDTDKDGNQLTLLDVVSCEDTISD
                     DVDLKIKSEKLHQYIKEALDNRERSIIQMRYGIDGQKPKTQREVAKLMGISRSYVSRI
                     EKKALQDLKVMFEKEENQKRPGKPIFQNQ"
     gene            complement(1626..3293)
                     /locus_tag="VHR42_07020"
     CDS             complement(1626..3293)
                     /locus_tag="VHR42_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX3026961.1"
                     /translation="MNNQVIHVDTDKCVGCNQCIKVCPLDQVNSAYSEGNQVKVRPDR
                     EKCIHCGACLEVCCHDSRLFTDDTEDVLNHIHNGKSFIVLAAPSIRVNFKDSWERLIS
                     TFKQLGAKAVYDVSVGADICTWSHVKYFQENGVHSIISQPCPVIVNYITKYRPDLIGV
                     LSPIHSPVVCLSVFLRKYMHENEKIIFLSPCVSKSDEFRLTGVDAFNFTFENIEQCLE
                     DGRLNLSGEPGEFDNMEPGMGRIYSRPGGLKENIEYYLGKELSVCNVEGTGKVFRFFD
                     EIKKGETENLPDILDVLNCEDGCNYGTGCSNSLGRLETFQIMNEQQELSAQLYPKTAD
                     SSTRLFDYFDKRLDLNDFIRAYQAVGVEETAMEVMDLEEAFDSLDKHTFEERNFNCGA
                     CGSHTCEEMARKISRGLNVPENCVWKTKRELSENSRMVAGFSVELTRDVSRITEELSE
                     AVHSLSAKNQEMASAVRIINSISTTTRLLSLNAGIEAARAGNAGKAFGVVADEIKKLA
                     DESAEAASEIEKVVGENLTLTGEAVHKLDDVDKIIVQIRDKVEELNI"
     gene            complement(3320..3904)
                     /locus_tag="VHR42_07025"
     CDS             complement(3320..3904)
                     /locus_tag="VHR42_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEX3026962.1"
                     /translation="MTVNKEVLDTNDLHINVSELKQCAGRLKAGSRILLSGTVYTARD
                     AAHKRIVGLIGEGGSLPFEIRDAVIYYAGPTQAPEHLCIGSCGPTTSSRMDVYTPQLL
                     NMGLAGMIGKGERSSEVCDAIRRNGAVYLCALGGAGALACECIKSCEVIAFEDLGCES
                     VKKLSIVRFPLFVAVDCNGGNIFETGRAKWANPL"
     gene            complement(3945..4832)
                     /locus_tag="VHR42_07030"
     CDS             complement(3945..4832)
                     /locus_tag="VHR42_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026963.1"
                     /translation="MNEGVFKTSVVGGFRKSDVLEFVDQLDAHYKQQEQQLNQTIEKT
                     REDFKKSEEENGRLREQVKDFEAELEIQKENIRVLQDQKRETDAEISRLTEESDRKDR
                     EIDSQRSGNLLLQHRLASSNQESGEKDKKISELEEKAEALEATIENKNKTEDQIGRVM
                     LEARSAADRMIDEANAKSRQIVQQAYDKIGGLTGDVREFKQKVTGIHESTKEFFQTVD
                     SMLNAIEESADLLDCEYIELVNSAVDGERGAEPSKTVFLEENGVQGDVSFCGSPVEGN
                     DCESGREDCGFAEDAGGTN"
     gene            complement(4917..>5241)
                     /locus_tag="VHR42_07035"
     CDS             complement(4917..>5241)
                     /locus_tag="VHR42_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3026964.1"
                     /translation="VFIALVDILPVLGTGTVMIPWAIIEMITGDVTMGLSIGVLYGVI
                     TLIRNIIEPKLVGYHIGLHPLVTLMAMFIGLSVMGVPGMFLFPITLIILKHMQDTGKI
                     RIWKD"
BASE COUNT         1337 a         1423 c         1087 g         1394 t
ORIGIN      
        1 atgatcattg gttttctaat tctgaaactt tttctgaaat gtacaaaaag atggctgggc
       61 ttagatttat tggagcagat caataatccg aagtcaatga atgccatcaa aaatactccg
      121 gtttgatttt taaagagtat ctaaataaaa aatccaaatt actgtaatca tacaggcggc
      181 cgtgtagaac ggccgcctgt ttaaaccttt catgggcgaa aggattactc cactctgctc
      241 tggattcttt tgagccgtgt aatcgactgc tcaatatatg tttccgggtc attgcagtac
      301 cggtcatcaa gctcaatgct tccatagccc ttataaccgt accggttgac cagccgaaaa
      361 agaccgtcaa aattgaactc cccgtcgccc accagcagat gagaatagct gattccatcg
      421 ccgtcggcca gatggagatg tcccatctta ttcttgagct tgataaaata atcggacatc
      481 ggctcatgaa cgaccagcgg gctgacacag tcaagcatgc tccggaaata ttccgaaggc
      541 acctcaccga gcagtctgag caaatcgttt gttgttgtaa gaatcagact ttcataaggt
      601 gtcagcgctt ccatgacaat cccgacccct acggattcgg catgctcggc gatttcccgg
      661 cagccttcca ccaaacgggc ccagtccttt tggtaatccg agccgtaacc gggatcgccg
      721 ggggtaattt gaatcagcgg cgcgccaatc tctttggcga tatcaaggga aagcttgaaa
      781 tattcgatcg tttcccgtct ttccttctcc tcctgggagc agagattata aggataaaca
      841 cagacctcag gagtataaac actgatttca agaccagtgt tccttgccat tctgtggaca
      901 cgctctattc ttttgtcatc catatcaaag ggataagcgt gcggccttcc gccccaaagt
      961 tcgatgccct gatagccgaa cctcgcaccg tcctcaaatg ctttttccat cgaataccgg
     1021 cggtaaacgc tggtaagaaa tgaatatttc accgctatcg ctccttttgc aacgttttcc
     1081 ttattgtatc atgaaagtgc ccggattgac aaaacttttg tgtcaaccgc ggcaggatag
     1141 caaatgaaat actgatgtat ttcaggaatc agaaaaagtc ttcgcaggat gtttacatca
     1201 gtgacccaat cgataccgat aaagatggta atcagctcac gcttttggat gtagtaagct
     1261 gtgaagacac catatccgac gatgttgatc tgaaaatcaa gtcggaaaaa ctgcaccaat
     1321 acataaagga agcgctggac aacagggaac gttctattat tcagatgcgc tacggcatag
     1381 acggtcagaa gcccaaaact cagcgggaag tcgccaagct gatgggcatc tcccgatcct
     1441 atgtatcgcg tatcgaaaaa aaagctctcc aagacctgaa agttatgttt gaaaaagagg
     1501 aaaatcagaa gcggcccgga aaacccatat tccaaaatca ataacccatg tttgaaagca
     1561 ggaggccgtt aacggcctcc tgctttcgat tgtagagaaa attcatttta atttgaaact
     1621 gccgtttaaa tattgagttc ctcaacctta tccctgattt gcacaatgat cttatcaaca
     1681 tcatcaagtt tatgaacggc ttccccggtc agcgtcaaat tttccccgac taccttctca
     1741 atctcagacg ccgcttcagc gctttcatcg gcgagctttt tgatttcatc agcaactact
     1801 ccaaacgcct taccggcatt tccggccctc gccgcttcaa ttccggcgtt aagcgataaa
     1861 agccgggttg tcgttgaaat tgaattgatg atccgaaccg ccgacgccat ttcctgattt
     1921 ttggcggaca gcgagtggac cgcctccgaa agttcctcgg tgattctgga aacatctcgt
     1981 gtcagttcaa cagaaaaacc ggcaaccatc cggctgtttt ccgacagctc ccgcttggtc
     2041 ttccagacgc agttctccgg aacattcagc cctctggata tttttcttgc catttcctca
     2101 caggtatgag atccgcaggc gccgcaatta aaattccgct cctcaaaagt atgtttatcc
     2161 aggctgtcaa acgcctcctc aagatccata acctccatag ccgtctcttc gacgccaacc
     2221 gcctgatagg cacggataaa atcattcaga tcgagacgtt tgtcaaaata gtcgaacagt
     2281 cttgtggagg agtctgccgt ctttggatat aactgagccg acagctcttg ctgctcgttc
     2341 ataatctgaa aagtttccag cctgccgaga ctgttggaac agcccgttcc gtagttacag
     2401 ccatcctcac aattgaggac atccaaaata tcgggaagat tttccgtctc tcccttttta
     2461 atctcatcaa agaagcggaa gaccttaccc gttccctcaa cattacagac tgaaagctct
     2521 tttccaagat aatactcaat gttttccttc aaaccgcccg gtctggaata gatgcgcccc
     2581 ataccgggtt ccatattatc aaattcaccg ggctcgccgg aaagattcag tcggccgtcc
     2641 tccaaacatt gctctatgtt ctcaaacgta aaattgaaag cgtcaacccc ggttagacga
     2701 aactcatccg atttgctgac gcaaggcgag agaaaaatta ttttttcatt ttcgtgcata
     2761 tatttacgta aaaaaacgga cagacaaacc accgggctat ggatcggaga caacaccccg
     2821 attaaatcgg gccgatattt tgttatataa ttaacaatca cagggcaggg ttgagaaatt
     2881 atgctgtgga caccattttc ctgaaagtat ttcacatgac tccaggtgca gatatccgcc
     2941 cccactgaca catcatagac ggctttggct cccagctgct tgaaggtcga gatcagcctt
     3001 tcccaactat ctttaaaatt aacacgaatt gacggcgcgg ccagaacaat aaaactcttc
     3061 ccgttatgta tatggtttaa aacgtcctct gtgtcatctg taaacaggcg ggaatcatgg
     3121 cagcaaactt ccaagcatgc cccgcaatga atacattttt cccggtccgg cctcactttg
     3181 acctgatttc cttcagaata cgcggagtta acctgatcaa gcggacaaac cttgatgcac
     3241 tggttgcagc ctacgcattt gtctgtatcc acatgaatga cctgattatt caattgtatt
     3301 tcccccaaat tttacgctat caaagcggat ttgcccactt tgcgcgacca gtttcaaaaa
     3361 tgtttcctcc attacagtcc accgcgacaa aaaggggaaa cctaacaatt gacagcttct
     3421 ttaccgattc acatcccaga tcctcaaaag cgataacctc gcaggacttg atgcattcac
     3481 aggcaagcgc tccggctccg ccaagcgcgc acagatagac cgcaccgttt cgccggatcg
     3541 cgtcgcagac ttcactgctg cgctcccctt tgccgatcat tccggcaagc cccatattca
     3601 ggagctgcgg ggtataaaca tccatccgtg aggaggttgt cggcccacag gaaccgatgc
     3661 agagatgctc gggggcctgc gttgggcctg cgtaataaat taccgcgtcc cgtatctcaa
     3721 agggaaggct ccctccctcc ccgatcagac cgacgatgcg cttgtgcgcg gcatcccggg
     3781 cggtatagac agtacccgac agcaggatgc gagaacccgc cttaagccgg cccgcgcatt
     3841 gttttaattc acttacgttt atgtgcaaat cgttcgtatc caacacttcc ttgttcacag
     3901 tcaaaaatca ccagcggcag tacccaccgt cgtgcaaaag tttcttaatt cgttccacca
     3961 gcatcctcag cgaatccgca gtcttcccga ccgctctcac aatcgtttcc ttcgacgggc
     4021 gaaccgcaaa aggagacatc cccttgaact ccgttttctt caaggaatac cgtctttgac
     4081 ggttccgcgc ctcgttctcc gtccaccgcg gaattgacca gctctatgta ttcgcagtca
     4141 agcaaatcgg ccgattcttc aattgcgttc agcatactgt cgaccgtctg gaaaaattcc
     4201 ttcgtgctct cgtgaatgcc tgtgactttc tgtttaaatt cccttacgtc ccctgtcagc
     4261 ccaccgattt tatcataggc ctgctgaaca atttgccgcg atttcgcatt tgcctcgtcg
     4321 atcatccggt cagccgcgct tcttgcctca agcatcaccc gcccgatttg atcttccgtt
     4381 ttgtttttgt tctctattgt cgcctcgagc gcctcggcct tctcctcaag ttcggatatt
     4441 tttttgtctt tttccccgga ttcctgattt gaggatgcaa gccggtgctg gagaagcaga
     4501 ttaccgctgc gctgactgtc gatctcgcga tcctttctgt cggattcctc cgtcagccgg
     4561 ctaatctcgg catccgtctc ccttttctga tcctggagaa cgcgaatatt ttccttttgg
     4621 atttccagct ccgcctcaaa atccttgacc tgctcgcgca gacgtccgtt ttcctcttcg
     4681 ctttttttaa agtcttctct tgttttctcg attgtttggt tcagctgctg ctcctgctgc
     4741 ttgtaatgag cgtcaagctg atccacaaat tcaaggacat cgctttttcg aaagccgccg
     4801 accactgaag ttttaaacac gccctcgttc atactcaaac ctcccggttc tatttcctat
     4861 tttaatccat tagcatcaag tgattcaatc ttattatacc ataaggcggc ctacaatcaa
     4921 tccttccaga ttcttatttt cccggtatcc tgcatatgtt tgaggataat cagcgtaatg
     4981 ggaaaaagaa acattccggg cactcccatg acgctcagtc cgataaacat tgccataagc
     5041 gtaacaagcg ggtgaagtcc aatgtgatag cctaccagct tcggctcgat aatgttgcgg
     5101 atcagggtaa tcacgccgta aagcacccct atggaaaggc ccatggttac gtctccggtg
     5161 atcatctcta taatcgccca gggtatcata accgttcccg ttccgagaac cggaaggatg
     5221 tcgaccagag cgataaaaac c
//
LOCUS       DATALY010000257         5493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_55716,
            whole genome shotgun sequence.
ACCESSION   DATALY010000257 DATALY010000000
VERSION     DATALY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55716"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VHR42_07040"
     CDS             complement(<1..460)
                     /locus_tag="VHR42_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026965.1"
                     /translation="MSNLRVELTKHPKQKPSDQTRLGFGDIFTDHMFVMDYDKGEGWH
                     DARIVPYAPLPIDPAAMVLHYGQEVFEGMKAYRAKDGRILLFRPDENFKRLNVSNDRL
                     CIPAIDEKIALESLNELIKIEKKSPANLPAFFCILGDRFSIDRNFPAVWPD"
     gene            807..1886
                     /locus_tag="VHR42_07045"
     CDS             807..1886
                     /locus_tag="VHR42_07045"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX3026966.1"
                     /translation="MQNDTITVGLPLNQPTGKFKKKKRSLRQTFLLSFVSSVIGFLLT
                     GIFLLYGPISYFRVTWVTIAMSTLNHQWMATMLFDQKTISAILASNKTVEPNQSTNPL
                     EILSGKGSVKDNATTLPSSPEDGEHIINGIGFIKLKGSTYNGWMIKVYDPSRVTLGVS
                     KYLGTRGERVSDMAKRTGSFVAVNAGGFVDVGGQGLGGLADGICIADGVRVRYSSNKA
                     SPHSIIGIDSKSRLVLGKFTNYQIQEKNLLSAVEFKPLLIVNGEAAKLYGNGGWGTDP
                     RTAIGQTKSGVMLFLIIDGRRVSSLGATMKDVQDIMLEYGAYNAANLDGGSSSTLVVK
                     NQVINNPSSSGGERYVPNAFLIRYR"
     gene            complement(2077..3402)
                     /locus_tag="VHR42_07050"
     CDS             complement(2077..3402)
                     /locus_tag="VHR42_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX3026967.1"
                     /translation="MGKKILAVLLVACCIFIFAGCTTTEKGFAEILNTMGKQGDFSYQ
                     GTTHLGVSNTIYAKLFKQVSKNLADTVDVTLTYSGVVNAAKGRITFSADVSSIGGNLK
                     GLRVILENDEAYVSENCLISEGVKGYPVKKIDGQTYGVYKLQYLLDLILGATDSEQAQ
                     AAESYLKDMPLTTGQLISMAQSFLADSTSKDAIDSMGDFSLGDALTKAAGSDKYTLLL
                     TTAKVADLLEQQMGGAGDPGFEDWLSEFKSHVQFTLNYSLEKVGSSAYNHQMSFSLHL
                     LSDQDSIPQKTAGQKTLQNSMDFSSSASLTTYPVSMNTQQIATTLTSGELVLNVSGHD
                     IQNCGILYSTSPKMTNARRMNAAYKGGKYIVDLSGLSPNTVYYYQTFATNKSGDTLYS
                     ATSTFKTKAPAPNPNTGSSDQPYALYPAALIIFAGVCFGVIRKKASSAR"
     gene            complement(3530..4480)
                     /locus_tag="VHR42_07055"
     CDS             complement(3530..4480)
                     /locus_tag="VHR42_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX3026968.1"
                     /translation="MSGDKKIATRDAYGKALAELGEMYPELVVLDADLSKSTKTETFR
                     AKFPERHMNCGIAEGNMMSVAAGLAAAGNIVFASSFAMFAAGRAFEQIRNSIGYPHLN
                     VKIGATHAGLSVGEDGATHQCCEDIGIMRTIPGMIIINPSDAVEAREAVIAAAKYKGP
                     VYLRFGRLAVPVINDNPGYKFEIGKGVCLKDGSDVTIIATGLMVPEALKAAEMLTADG
                     ISARVVNIHTIKPIDREIILQAAKETGVLVTAEEHSVIGGLGSAVCETVAGGCPVPVF
                     MVGVNDTFGESGKAEEVLEAYGLSAGHIAEVAKKAVSLKK"
     gene            complement(4484..5326)
                     /locus_tag="VHR42_07060"
     CDS             complement(4484..5326)
                     /locus_tag="VHR42_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX3026969.1"
                     /translation="MEAAVKKQLAKIALDIRTHSVEAVYYAKSGHPGGSLSVADVLAY
                     LYFEEMRVDSKNPGWEDRDRLVLSKGHCTPALYSALSLKGFFPVCELKNFRKLGHMLQ
                     GHPDMKKVPGVDMSTGSLGQGISAACGMALSAKLSKKDYRVYTVLGDGELEEGQVWEA
                     AMFAAHYKLDNLCALVDFNCLQIDGDIRQVMSPLPIAGKFEQFGWNVIEIDAHDFEQI
                     DAAYKQARCCKGRPTVIVARSVKGKGVSFMENAPEWHGSAPNQEQFEQAMSELEAANK
                     QLEG"
BASE COUNT         1392 a         1458 c         1230 g         1413 t
ORIGIN      
        1 gatcgggcca tacagcagga aaattcctgt caatagaaaa ccgatcaccg aggatacaaa
       61 agaaagcagg aaggtttgcc ggagacttct tttctatttt aatcaactcg ttaagcgatt
      121 caagagcaat tttttcatca attgccggaa tacagagccg gtcattggat acattaaggc
      181 gtttgaagtt ttcgtcaggc ctgaaaagaa gaatccggcc gtctttggcg cgataagcct
      241 tcatcccttc aaagacttcc tgtccgtaat gaagaaccat ggccgcaggg tcgatcggca
      301 aaggagcata cgggacgatc cttgcgtcat gccagccctc gcccttatca taatccatca
      361 caaacatgtg atcggtgaaa atatcgccaa accccagtct ggtttgatcc gacggttttt
      421 gttttggatg cttggtaagc tccaccctta aatttgacat tgcttttcct tcctttaact
      481 aacaattttc agaacatttg gaatcatttt aattatacca gtgtaaaaac ggaattacaa
      541 gtgcaaatca agttcgggcg ggactccgca cacggtctgt gtcagaatca tttttccctc
      601 tttctgcata ggaatggtac tggtgttcca cgatcctgcg caagagcgcg aaaagcgtca
      661 gattttcacc aaatttacat tttatttata attctgtaat ctttcagaaa ttgcaatccc
      721 gcccgataac ttttataatg gatttattgc ggagcacccg cattttattt tgccacacaa
      781 aaacaaaaaa gattcggagt tttaatatgc aaaacgatac catcacggta ggcctcccgc
      841 taaaccagcc caccggtaaa ttcaaaaaaa agaaaagaag tctccggcaa accttcctgc
      901 tttcttttgt atcctcggtg atcggttttc tattgacagg aattttcctg ctgtatggcc
      961 cgatctccta tttcagggta acctgggtaa ccattgcgat gagtaccctg aatcatcagt
     1021 ggatggcaac catgctgttt gatcaaaaaa cgatcagcgc cattcttgca agcaacaaaa
     1081 ccgttgagcc gaaccagagc accaatccac tggaaattct gtctggcaag ggcagtgtca
     1141 aagataacgc gactaccctg ccctcctccc cggaagacgg tgaacatatc atcaatggaa
     1201 tcggtttcat taaacttaag ggctccacct ataacggctg gatgatcaag gtttatgatc
     1261 cttcccgggt cacgctcggc gtttccaaat atctcgggac caggggggaa cgtgtcagtg
     1321 atatggcgaa aagaacagga tcgtttgtag ctgtcaacgc gggcggcttc gttgatgtcg
     1381 gcggtcaggg actgggagga ttagccgacg ggatctgcat agccgacggc gtaagagtcc
     1441 gatattccag caacaaggcg tccccgcatt cgattatcgg tatcgacagt aagagcagac
     1501 ttgttctcgg caagtttacc aactatcaga tacaggaaaa aaatctcctc agcgcggttg
     1561 aatttaagcc tttgctgatt gtcaacggcg aagccgccaa actctacgga aacggcggat
     1621 ggggcaccga tccgcgaacc gcgatcggcc agacgaaaag cggagtcatg cttttcctga
     1681 tcattgacgg ccggagagtc agcagtcttg gcgccacaat gaaagacgtt caggatatca
     1741 tgctcgaata cggcgcatat aacgcggcga atctggacgg aggctcttcc agcacgctgg
     1801 tggtaaaaaa ccaggttatc aataacccct ccagttcggg cggagaacgg tatgttccca
     1861 acgctttcct gatccgttac cgttaaaatc ccagaccgcg caaaaatact tctcttaaat
     1921 tacaaaaatc gattcacccc cgcctgcaaa catatcaacg gcaaatccgc tttagaattt
     1981 tggacagggg gcaaaagggt tgatccgtat agaaaacggg ccgcagtcag cggcccgttt
     2041 ttcctgtcat attttgttgg ctgcccgggt aacgtttcag cgtgcggaag acgctttctt
     2101 acgaatgacc ccgaagcaga cgcccgcgaa aataatcagc gcggccggat acagtgcata
     2161 gggctggtcg gagctgcccg tattggggtt tggcgccggc gcttttgtct tgaaggttga
     2221 tgttgcactg taaagagtat cgccggattt attggtagcg aaggtttgat aataataaac
     2281 cgtgttgggg cttaagccgc tcaggtcaac gatatacttc ccacccttgt aggcggcgtt
     2341 catccttctg gcattggtca ttttcgggct tgtcgaataa agaataccac aattttgaat
     2401 gtcatgaccg gaaacattca gcaccagctc cccgctggta agcgtcgttg cgatctgctg
     2461 tgtattcata ctcaccggat aggttgtcag gcttgcggaa gacgagaaat ccatcgaatt
     2521 ctggagcgtt ttctgacccg ccgttttctg ggggatcgaa tcctgatccg acagtagatg
     2581 cagcgagaaa gacatctggt gattatacgc gctcgagcct actttttcca gtgaataatt
     2641 caaagtaaac tgaacgtgac ttttaaattc gcttagccaa tcttcgaaac ccggatctcc
     2701 tgcgccgccc atttgctgtt ccagcaaatc agcgacttta gcggtggtaa gcaagagagt
     2761 atatttgtcg gaaccggccg ctttggtaag cgcatcaccc aatgaaaaat cacccatgga
     2821 atcaatggca tctttactcg ttgaatctgc caggaaactc tgcgccatgc tgatcagctg
     2881 gccggttgtc agcggcatat ctttaagata gctttcggca gcctgcgcct gctcactatc
     2941 cgtcgcccct aaaatcaagt ctaaaagata ctgcagctta tagactccat aagtctgccc
     3001 gtcgatcttt ttaaccggat agcctttgac tccctcactt atcaggcagt tctccgaaac
     3061 atatgcttca tcgttttcaa gaatcactct gagtccctta agatttcctc ctatggagga
     3121 aacgtctgcg ctaaatgtaa ttcgcccttt tgcggcattt acgacgccgc tgtaagtcag
     3181 cgtaacgtcc accgtatcag ccagattctt gctcacctgc ttaaagagtt ttgcatatat
     3241 ggtgttgcta acccccaaat gggtcgttcc ctgataggag aaatccccct gtttccccat
     3301 cgtattcaga atctctgcaa atcccttttc tgttgtggtg caccctgcaa aaataaagat
     3361 gcagcaagca accaataaaa cggctaagat tttctttccc atataaaccc tcctaaaatt
     3421 gtcagggata tattaccaca ggcaagccgt ttcgtcaata taatctggaa atattgtaaa
     3481 aaaaagcaag cctgatcagg cttgctttta tgaaaaatat tgaaaatagt tattttttga
     3541 gggaaaccgc ttttttcgcc acctcggcaa tatggcctgc gctgaggccg taggcttcca
     3601 gcacctcttc ggcttttccg gattcgccaa acgtatcgtt caccccgacc atgaataccg
     3661 gcacagggca gccaccggcg accgtctcgc agactgcgct tcccagcccg ccgatcacgc
     3721 tgtgttcctc ggccgtgaca aggactcccg tttcttttgc agcctgaagg atgatctcac
     3781 ggtcaatcgg tttaatcgta tgaatattga cgacacgcgc gctgatccca tctgccgtga
     3841 gcatctccgc ggctttgagc gcctcgggaa ccatcaggcc ggtcgcaata atggtaacat
     3901 cgctcccgtc cttgaggcaa acgcccttcc cgatttcaaa tttgtagccg ggattgtcgt
     3961 tgatgactgg tacggccagt ctgccgaacc ttaaataaac cgggcccttg tacttcgccg
     4021 ccgctataac cgcttctctt gcttccaccg cgtcggaagg attaataatg atcatacccg
     4081 gaatcgttct cataataccg atatcctcgc agcactgatg ggtggccccg tcctccccga
     4141 ccgaaagacc ggcatgcgtt gcgccgattt tcacattcaa atgaggataa ccgatagaat
     4201 ttcggatctg ctcaaacgcc cttcccgccg caaacatggc aaaagagctt gcaaacacaa
     4261 tatttccggc agccgcaagc cccgcggcta cggacatcat gttgccctcg gcgattccgc
     4321 aattcatatg tctttccgga aacttcgctc tgaaagtttc cgtttttgtg gattttgaaa
     4381 gatccgcatc cagcactaca agttcgggat acatttctcc aagctctgca agagcttttc
     4441 cataggcgtc acgggtcgct atctttttat cacctgacat tctttaaccc tccaactgct
     4501 tattggccgc ttccagctcg ctcatagcct gctcaaactg ttcctggttc ggcgcggacc
     4561 catgccactc cggtgcattt tccataaacg atacgccttt gcccttgaca ctgcgcgcca
     4621 cgatgacggt aggccggccc ttgcagcatc ttgcctgttt ataagcggcg tcgatctgtt
     4681 caaaatcgtg ggcgtcaatc tctatgacgt tccatccaaa ctgctcaaat tttccggcaa
     4741 tcggcagagg agacataacc tgccggatat ctccgtcgat ttgcagacag ttgaaatcaa
     4801 ccaaagcgca taaattgtcc agcttataat gagccgcgaa catggccgct tcccagacct
     4861 ggccctcctc cagctcaccg tcgcccaaca ccgtgtaaac ccggtaatct ttcttcgaga
     4921 gtttggccga cagggccatc ccgcaggcgg cggaaatccc ctgccccagc gaaccggtag
     4981 acatatccac gccgggcacc tttttcatat ccggatgccc ctgcagcata tggccgagct
     5041 tccgaaaatt ctttaattcg cagacaggga aaaatccttt cagcgacaaa gccgaataaa
     5101 gagccggcgt gcagtggcct ttggaaagca cgagcctgtc cctgtcttcc caaccgggat
     5161 tttttgaatc cactctcatt tcctcaaaat ataaatatgc cagaacgtcg gctacggaca
     5221 atgacccgcc cggatgaccg gattttgcgt aataaacagc ctcaaccgaa tgtgtcctga
     5281 tatcaagagc tatttttgcg agctgttttt ttactgctgc ttccatgctg ctgcctccat
     5341 ctacttaaaa taatgccgct ggtccaattt agaaaaacga tctgattctg atattattga
     5401 ccggatttta accgcgtttc aaatttttct tcaagataat caatcaaatc ggcgagtgct
     5461 ccgtcttcgc accggccgac gaccagattc gcc
//
LOCUS       DATALY010000258         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_660926,
            whole genome shotgun sequence.
ACCESSION   DATALY010000258 DATALY010000000
VERSION     DATALY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660926"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..345
                     /locus_tag="VHR42_07065"
     CDS             46..345
                     /locus_tag="VHR42_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026970.1"
                     /translation="MEKATEKKLLPELLCAAIRHFRSGEDAAGMEELLKAVDDLESAA
                     EAEQGFQQFQFHKLLPALRELNQFMQNQDIAGMTDWLEYTAVPMTEDRPEGYGET"
     gene            342..962
                     /locus_tag="VHR42_07070"
     CDS             342..962
                     /locus_tag="VHR42_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026971.1"
                     /translation="MKIAEPDKIEEMYEKNLSLLTPWLRETLAKISLEELNEKIEVTY
                     NSEGYPVCRYHRDGLCFHVTSENPVQEAEAWYRSVQHVGSSETILYGSGFGYALFEVF
                     AHKVPHTLVVVFEQDLFLFKAMLCYFDLSPIIETQKILFLIGDSSFFKKAFEELFFTM
                     QFFQYDVPEHSEDSAGDAQFQKGISGNSPVYLQGTNFADFLPWERS"
     gene            871..2271
                     /locus_tag="VHR42_07075"
     CDS             871..2271
                     /locus_tag="VHR42_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HEX3026972.1"
                     /translation="MRNFKKEYQEIHRFIYKELTLLTSYLGNDHKDNMIGLRNLAANT
                     KTVLENPYVNCLKDQYKNVPAFIISNGPSLDLSMPLLKGIQGRGLMIAVESAIVPLTA
                     NGINPDILAVQERTKYTYLYHFEGRNYSPDISLLALALVDPRVFPSFPGEKVPIFRKG
                     EELNHWFSKYLAESSELDAGANVSHLAAEEAVYLGADPIIFVGQDFAYGPGGVTHSRD
                     AVASQEKGKRARDLLHAIPTVYVEGNNGEMIPSNRLWDNFRLGLEHIIELYPGHRFYN
                     ATSGGAKIRGTEYAKLDQLIRQYCTQPIPYRVNEIIAENRKMISHAERKKLLEKFITE
                     MEHLAVTFRELTVETNQNKLECEKMMFLCSEEGQEKNYRLLNETYQTNIAAFYRYSGN
                     ELCRSFFQQLNCAYFHLLDRLGEIDTMAKMEQAFGIQSQFFHDLRVVCQSLSITLEKV
                     VLDLHALFEEYRKKGE"
     gene            2274..3260
                     /locus_tag="VHR42_07080"
     CDS             2274..3260
                     /locus_tag="VHR42_07080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3026973.1"
                     /translation="MEEEAQLTLCVITKNDAAFLPVCLNEMKDVADEILVADLGSEDH
                     TPELAQQAGATVYRLKWENDFSQIKNFCMEHASGKWVLFLQADEILPMEQHAELNLLL
                     KNPNAEGYLLYVDYNQEERGISSPAQFLRLIRNRKEYRFHYRSFPYIPDEVLYSLQNC
                     SLRITHRGEKTVGWQIREKQTLLKEDLREYPTDCYLRYMEGIELLNREKFEESTESFE
                     EARQAINGGYLYVPHLYKCYGYALIALGRLEKAEEILTEGVQYFPFYNDLLVLRSELR
                     HQCGQSQKALEDLKTCMALRSKLNPYVPAPEIDPAILQEMWEELISDPGQEQ"
     gene            3404..3772
                     /locus_tag="VHR42_07085"
     CDS             3404..3772
                     /locus_tag="VHR42_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4183 domain-containing protein"
                     /protein_id="HEX3026974.1"
                     /translation="MAIKKLFQLAATTPVVSLTDTNYFYTVTAPITLTANASFVLSKS
                     KWSNGAGTTPASFATKSNGYYNMFINGVLQQSVMFTVNTISAVHLINAGTASITVPLN
                     APITLGLAKSSVAPAKVVVP"
     gene            4520..>4769
                     /locus_tag="VHR42_07090"
     CDS             4520..>4769
                     /locus_tag="VHR42_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026975.1"
                     /translation="MNLSPTECFIIIVAFLAIGEFIATKTKAYMPSVFIAALCCMVGF
                     WTFLPKDVVTQASFGPNFVSICLSLLLVHLGTLMSVKKL"
BASE COUNT         1321 a         1060 c         1145 g         1243 t
ORIGIN      
        1 atcatttatc caagtatcag ctagagggat ggagggaatt agttgatgga gaaggcgacg
       61 gaaaagaaac tcctgccgga acttctgtgt gcggcgatcc gtcactttcg cagcggtgaa
      121 gacgctgcgg gcatggagga gctgttaaaa gctgtggatg acctggaatc tgcggctgaa
      181 gccgagcaag gattccagca atttcagttt cataagctgc tgcccgccct gcgggagctg
      241 aatcaattta tgcagaacca ggatatcgcc ggtatgaccg actggctgga atatacggct
      301 gttcccatga ccgaagatcg gccggaagga tacggtgaaa catgaagatt gcggaaccgg
      361 ataaaataga agagatgtat gaaaagaacc tttcgctgct gacaccgtgg ctaagggaaa
      421 cgttggccaa aatttcactg gaagaactga acgaaaaaat cgaggtcacc tataattccg
      481 agggctaccc cgtctgccgg tatcacagag acgggctttg cttccatgtt acaagcgaaa
      541 atccggttca ggaagcggag gcatggtatc gttccgtcca gcatgttggt tcgtcggaaa
      601 ccatactcta cggcagcggt ttcggatacg ctctgtttga agtttttgcg cacaaggtgc
      661 cccatacgct ggtggtcgtg tttgaacagg atcttttcct ctttaaggca atgctgtgtt
      721 attttgatct ctcaccgatt attgaaacgc aaaagatatt gtttctcatc ggggacagct
      781 cctttttcaa aaaggcgttt gaggagctgt tctttaccat gcagtttttt cagtacgacg
      841 tacccgagca cagcgaggac tctgccggcg atgcgcaatt tcaaaaagga atatcaggaa
      901 attcaccggt ttatctacaa ggaactaact ttgctgactt cctaccttgg gaacgatcat
      961 aaagataata tgatcggcct tcggaatctg gcggccaaca cgaaaacggt tctggagaat
     1021 ccgtatgtga actgcctgaa ggatcaatat aaaaacgttc cggcatttat catttccaat
     1081 gggccgtcgc tggatctcag tatgccgctg ctcaagggga ttcagggccg tggcttgatg
     1141 attgcggtgg aatccgctat cgtgccgctt acggcaaacg gaatcaatcc ggacattctg
     1201 gcggttcagg aacgaacaaa gtacacctat ctgtatcatt ttgaaggccg aaattattca
     1261 ccggacattt ctcttcttgc gcttgcgctg gtagacccaa gggtttttcc ttcctttccc
     1321 ggagaaaaag tcccgatttt ccgtaaggga gaggaactga accactggtt cagcaagtat
     1381 ttggcggaat caagcgaatt ggacgcagga gcaaacgtat cgcatcttgc tgcggaagaa
     1441 gccgtgtacc tcggcgccga tcctattatc ttcgtcggcc aggattttgc ctatgggcct
     1501 gggggtgtga ctcacagccg ggacgcagtc gcctcacagg aaaagggaaa acgtgcccgt
     1561 gacctgctcc acgccattcc gacggtttat gtggaaggaa acaacgggga gatgatcccc
     1621 tccaaccggc tttgggataa ttttcgactt ggtctggaac acatcatcga gttatacccg
     1681 ggacaccgtt tttacaacgc tacttccggc ggagcaaaga tacgcggaac cgaatatgca
     1741 aagctggacc aactgatccg gcaatactgc acccaaccga ttccgtaccg ggtcaatgaa
     1801 atcattgcag aaaacaggaa gatgatttcc cacgcggagc gcaaaaagct gctggaaaaa
     1861 ttcatcacgg aaatggagca tttagccgtt acattccggg agctcaccgt ggaaacgaat
     1921 caaaataaat tagaatgcga aaaaatgatg tttttatgtt cggaagaagg ccaggaaaaa
     1981 aattacaggc tattaaacga aacctatcaa acgaacattg ctgcatttta tcggtattca
     2041 ggtaacgaac tgtgccgcag cttttttcag cagctgaact gcgcttattt tcatttactg
     2101 gaccgtctgg gagaaatcga taccatggca aaaatggagc aggcattcgg cattcaaagt
     2161 cagttctttc atgatctgcg tgtcgtgtgt caaagtttat ccattacttt ggaaaaggtt
     2221 gtcctggatc tgcatgctct tttcgaagag taccgcaaaa agggggaata aaaatggagg
     2281 aagaagcgca gctgaccctt tgcgtcatta caaaaaacga cgcggctttt ctgcccgtct
     2341 gcctgaatga aatgaaagac gtggccgacg aaatactggt cgcggatctc ggctcggaag
     2401 accatactcc cgaactggct caacaggcag gtgcgacggt atatcggtta aaatgggaaa
     2461 acgatttcag tcaaattaaa aatttttgca tggagcacgc ttccgggaaa tgggttctat
     2521 ttttgcaggc ggacgaaatt ctgccaatgg aacagcatgc agaactgaat cttttgctga
     2581 aaaatcccaa cgcggagggc tacctccttt atgtggatta taatcaggag gaacgcggca
     2641 tttcgtcgcc cgcgcagttt cttcggttga ttcgcaaccg gaaagaatac cgcttccatt
     2701 atcgctcttt tccgtatatt ccggatgaag tattgtattc tttgcaaaat tgcagccttc
     2761 gcattacaca ccgcggagaa aagaccgttg ggtggcaaat aagggaaaag caaactcttt
     2821 tgaaagagga tttgagagaa taccccaccg actgttacct tcggtatatg gaaggaattg
     2881 agcttctgaa ccgagaaaag tttgaggaaa gcacagaatc gttcgaagag gcacggcagg
     2941 caattaacgg tggatatctt tacgttccgc atttatataa atgctacggt tacgctctga
     3001 tcgcactggg tcggctggaa aaagccgaag aaattctaac ggaaggcgtt caatattttc
     3061 ctttctacaa tgacctgctg gtcctccgat ccgaactgcg tcatcagtgc ggccaaagcc
     3121 aaaaagctct ggaagatttg aaaacctgta tggctcttcg gtcaaaattg aacccctatg
     3181 tacccgcacc ggaaatcgat cctgccattc ttcaggaaat gtgggaagaa ctcatatcgg
     3241 acccggggca agaacaataa agtccacctc ttttactatc acacgaaggg gacgccgcat
     3301 agaatgagat atgcttaata ccgatgctct gcctctgaaa gattccatat cggtaagaaa
     3361 attgcatcag tattaaagca atagaaagga agagagaata actatggcaa ttaagaaact
     3421 gtttcaactg gctgctacga ctccggttgt atcgttaaca gacacgaatt acttttatac
     3481 agtaactgcg ccgatcacgt taactgcgaa tgccagcttc gttctttcaa aatcgaagtg
     3541 gtctaacgga gccggtacga cccctgcctc ttttgctacg aagtcgaacg gctattacaa
     3601 tatgttcatc aacggcgtgc ttcagcaaag cgttatgttc acggtcaata caatttccgc
     3661 tgtccatctg attaacgcgg gcactgccag cattacggtt cctttaaacg cccctattac
     3721 ccttggactc gcgaagagca gcgtcgctcc ggcaaaggtt gtcgttcctt aaaagcaaca
     3781 ggctgctcct caatcaataa aaagttacag cgccccccga tcggcaaatg ccggtcgggg
     3841 gttttttcgt taagtgcatt ggcgctcctt gccttcgcgt ccaagcactc acccaagctt
     3901 cgaatgaccc ctcgctctcg cattaagaaa ataaacccaa actgttactt atgagaagta
     3961 gcaacaatca tattcaagca aaaattaatt ccatatttgc acgaattgta ataatctata
     4021 aaaaatactt aaaataattt gacagttcat atagattctt gtaaaataaa ggaatagtat
     4081 gcgatttcag gtggaaatat cgctatcgag aaaataaatt aaactttaca cagtttgttg
     4141 gaaaaagaca tctttttttt gattcttaaa ccatttatcc aggtcgtaaa gttttctaat
     4201 gattgatcgg ctgttccagc ttaatcggga cagtttttgg gtttttgttc cccatttgct
     4261 ttagcgcacc aacgttataa atcctttatt gttttaccgc tttatttgct aaaagcaaga
     4321 aaaaatctgt tctttagatg cgtgaaatga cagtgcggtg ggaaatttct tcgtccggca
     4381 aggtgaacga cacgggaatc tttgcgtatt cccggctttc tgcgagcatg gcggaaaatg
     4441 actgctgtaa tgatgttgag gctatgggaa ccggtttgaa aacttgtttt agaaaattat
     4501 tggattgagg ggattttcag tgaatcttag cccaacagaa tgtttcatta ttatcgtcgc
     4561 ctttttggcg atcggcgagt tcatcgccac aaagaccaag gcgtacatgc cttcggtatt
     4621 tattgcggca ctgtgctgta tggttggttt ctggacgttt cttccgaaag acgttgttac
     4681 acaggcttcg ttcgggccga atttcgtttc tatctgcctg agccttctgc ttgttcacct
     4741 gggaacgtta atgagtgtga aaaaactgc
//
LOCUS       DATALY010000259         6558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_487214,
            whole genome shotgun sequence.
ACCESSION   DATALY010000259 DATALY010000000
VERSION     DATALY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487214"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1802
                     /locus_tag="VHR42_07095"
     CDS             147..1802
                     /locus_tag="VHR42_07095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX3026976.1"
                     /translation="MPVHIISLGGLNEIGKNLTAYECQDDILIVDCGLAFPDDEMLGI
                     DLVIPDITWLEKNRDRVRDIVLTHGHEDHIGALPYVLKSLNVPLYGSKLTLGLVEGKL
                     KEHGLSGKVKLNVVQPGDKVKFGCMSVEFINVNHSIAGSFSLAITTPAGLFVHTGDFK
                     IDCTPIHGEMIDLARFGELGNQGVLALLMDSTNAERPGYTMSEKTVGESFDNLFKKAE
                     GKRIIIATFASNVHRVQQIVNAAQKYGRKVAISGRSMVNVCSVATELGYLNIPEGMAV
                     EIDAINRYPQDKLVIITTGSQGEPMSALYRMAYSDHRNVEVGPNDFIIISASPIPGNE
                     KTVSKVINELLKLGADVIYESLAEVHVSGHACQEELKTIMGLTKPKYFIPVHGEYKHL
                     KKNAGLAKSMGIPPQNILISDIGKVIEIDSQNIKITGSVPAGKVLVDGLGVGDVGSIV
                     LRDRKHLAQDGLIVVVATIDGSNASLVAGPDIVSRGFVYVRESETLMDQTRELAKNVL
                     IDCAEAGITEWSTIKSRVKDALSKLLYDKTKRSPMILPVIMEV"
     gene            1834..3798
                     /locus_tag="VHR42_07100"
     CDS             1834..3798
                     /locus_tag="VHR42_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX3026977.1"
                     /translation="MRGKKVWNLGAPVFLALGVLLIVSALSLFWDFRIFLAEIIACLV
                     VFAFTLLKMRNIHRNIRAMLVRTAEKLSPADRESLSVFPLPAVVVGKKGEVMWYNDRF
                     RNYVLNRKDAYGTYIRSIARGLSVEAFDKPGGTAEATVDSRKFTVYGSLAPAGGFYTL
                     YWVDNSELKNTALEYHESRPVVAIVMIDNYDELMQNAKEGEKADILSRVEKQLMAWVS
                     TTTGFLWHSDRDKYIFVFEERHYRKIVEERFDILDKVRQIIAGERMPATLSIGVGRGG
                     KSLAENEEMARQALDMALGRGGDQSAVKTRDGYEFYGGISKAVEKRTKVKTRIIASAL
                     AELIEGSENVLIMGHKNSDLDVVGACVGIYRSVVSRGIFAKIVINRRSSMAAEFISEL
                     EGRGYGEAFIDPEDSLLLVSKKTLLIIVDTHRKAFVDSPELYQNVKTVVVIDHHRKMV
                     DYIDNAVIFHHEPYASSASEMVSELVQYLADSSLTSVEADAMLAGITLDTRNFTVRTG
                     VRTFEAAAYLRRKGADTVRVKQLFAGSMENYRKRSKLVSSAEIYHGCAISRYDGDPDD
                     SLKFVTPQAADELLTITGVSASMVAYPAGSGVAISARSMGEINVQLIMETLGGGGHLT
                     MAGAQMDNITMAEAVHRIKEAVDRYFAENG"
     gene            3890..4339
                     /gene="rplI"
                     /locus_tag="VHR42_07105"
     CDS             3890..4339
                     /gene="rplI"
                     /locus_tag="VHR42_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX3026978.1"
                     /translation="MKVILKADLKGTGKAGELINVSDGYAKNYLLPRGMAIQADSKAI
                     NELRGKEESQKHRQEMEKEAAEKMADKLKDAVVKVFAKAGQNGKLFGAVTSKEIAQAV
                     KQQFDFNIDKRKIVLDGDIKAFGTYTTEIKLFSGITTSIRVVVGEKE"
     gene            4376..5740
                     /gene="dnaB"
                     /locus_tag="VHR42_07110"
     CDS             4376..5740
                     /gene="dnaB"
                     /locus_tag="VHR42_07110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX3026979.1"
                     /translation="MAQGKSYYEIGDSGFDGLALPYSLEAEQSVLGSILIDPASILQV
                     LEYLKPQYFYLPQHREIFTVMVRMFSESSVIDFVTMLDELKSQGVYDEAGGKAYLMQL
                     AQMVPSVANIEAYAKIVQEKYFLRSLITASREIISSAAEEAGDARMILDSAEQKIYDI
                     RQDKGADGLRHIKDIIIETYDRLHAISNDEDGRFVGTPTGFSELDRLITGLNRSDLIL
                     LAARPAMGKSAFAINIAANVAKRGEKVAFFTLEMTREQNVERMLSSESLIENRALRTG
                     ELKADDWVRLAQAADELSKYQLYFDDTSGITIPEMKAKCRRIKGLGLVVIDYLQLLSS
                     GRRIENRVQEVSEMTRNLKIMAKDLDVPIITLSQLSRGTESRQGHRPMLSDLRESGSI
                     EQDADIVMFLYREDYYEQNEQNHNQATCIVAKNRHGAVGDVPLHWDGAHTKFTSQEKY
                     RDEP"
     gene            5730..6080
                     /locus_tag="VHR42_07115"
     CDS             5730..6080
                     /locus_tag="VHR42_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026980.1"
                     /translation="MNLKAWKTIEKHHMMEQGGAVLAGVSGGADSVALLHLLCSLRGP
                     LGIRVYAAHINHMIRGAEALRDEQYVEKICREWGVELFVIRADVRREAAESGESLEEA
                     GRRIRYDYFEKKSL"
     gene            6052..>6558
                     /locus_tag="VHR42_07120"
     CDS             6052..>6558
                     /locus_tag="VHR42_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3026981.1"
                     /translation="MIILKKKACELGSGIATAHTLSDSMETVLINLARGTGLKGLCGI
                     PPVRGKIIRPLIECTRQEIVAYCHEHRLEYVVDSTNLSRDYVRNQLRLDVIPIFYRIN
                     PAFDREIARMTRQLGEDSDYLSSEAEKALKNASLGDSSYRAETLLRLPGPIVSRVLLL
                     AVKEFSGIG"
BASE COUNT         1665 a         1534 c         1871 g         1488 t
ORIGIN      
        1 cgaaccgcgc ggctaatccg gcgaaccgca cggctaatcc ggcgaaccac gcggccaacc
       61 cggcgaacca cgcggccaac ccggccgctt cgcacacagg ccagcgcagg ccttccaggg
      121 gcagacggtc gccgtccaag ccctctgtgc cggtgcatat tatttcgctg ggcggtctga
      181 atgaaatcgg taaaaacctg acggcctatg aatgtcagga tgatattctg attgttgact
      241 gcggccttgc gttcccagac gacgagatgc tgggaattga tctggttatt ccggacatta
      301 cctggcttga aaaaaacagg gatcgtgtca gggacattgt cctcacccac ggtcacgagg
      361 atcatatcgg agccttgccc tatgtgctca aaagtttgaa tgttccattg tacggctcaa
      421 agctgacgct tgggctggtg gaaggaaaac tcaaggagca cgggctgagc ggaaaagtga
      481 agctgaatgt cgttcagccg ggcgataagg tgaaattcgg ctgcatgtcg gttgaattca
      541 tcaatgtcaa ccattcgatc gcggggtctt tttccctggc gatcactacc ccggcgggct
      601 tgtttgtcca tactggggat tttaagattg actgtacccc gatccacgga gaaatgattg
      661 accttgcacg tttcggagag cttggcaatc agggcgtgct tgctttgctg atggattcca
      721 caaatgccga gcgcccaggc tacaccatgt cggaaaaaac ggttggcgaa tcgttcgaca
      781 acctttttaa gaaggcggaa ggcaagcgca ttattattgc gacgtttgcc tccaatgtcc
      841 acagagtgca gcagattgtc aacgcggcgc agaaatatgg ccgcaaggtc gccatatccg
      901 gccgcagcat ggtaaacgtc tgctcggtgg cgacggagct gggttatttg aatattccgg
      961 aaggcatggc ggtcgagatt gacgccatca accgctatcc gcaggataaa ttagtaatta
     1021 tcacgaccgg cagtcagggc gaaccaatgt ccgcactcta ccggatggcg tattctgacc
     1081 acagaaatgt ggaggttggg cccaacgact tcattattat ttccgcaagc cccattcccg
     1141 gcaatgagaa aaccgtttca aaagtgatca acgagctttt gaaactcggg gccgacgtga
     1201 tttatgagtc gcttgccgaa gtccatgttt ccggtcacgc ctgtcaggaa gaactcaaaa
     1261 ccatcatggg cctgacaaag cccaaatatt ttatccccgt ccatggtgaa tacaaacacc
     1321 tgaaaaagaa cgcggggctt gccaaatcca tgggcatccc gccgcagaac attttgatca
     1381 gcgacatcgg caaagtgatc gaaatcgatt ctcaaaacat aaaaataacg ggatcagtcc
     1441 cggccggcaa ggttctggtt gacggtctgg gagtcggcga tgtaggcagc attgtgctgc
     1501 gcgaccgcaa acatcttgct caggacggct taattgtcgt tgtggcgacc attgacggga
     1561 gcaatgcctc actggttgcc gggccggata ttgtttcgag aggttttgtt tatgtcagag
     1621 aatccgaaac cctgatggat cagacccgcg agcttgccaa aaacgtgctg attgattgcg
     1681 cggaagccgg tattaccgaa tggagcacca tcaaatcccg cgttaaggac gcgttgtcaa
     1741 aactattgta tgacaagacc aaacgaagcc cgatgatact tccggttatc atggaagtat
     1801 agccggctcc ggggccttag gaggaaatgg atcttgcgag ggaaaaaagt ctggaattta
     1861 ggggcgccgg tattcttagc cctgggagtt cttcttattg tttcagcatt gagtctgttt
     1921 tgggattttc gcatctttct tgcggaaata attgcctgcc tcgtcgtttt tgctttcacg
     1981 ctgctgaaaa tgcgcaatat tcacagaaat atacgcgcga tgcttgtccg aactgcggag
     2041 aagctcagcc cggccgacag ggaatcgcta tcggttttcc ccctgccggc cgtggtggtg
     2101 ggcaaaaagg gcgaggtgat gtggtataac gaccggtttc ggaattatgt cctcaaccga
     2161 aaagacgctt acggtactta tatcaggagc atcgcccgcg gtttgagtgt cgaggctttc
     2221 gacaagcccg gcggaacggc ggaggcaacg gtcgacagcc ggaaattcac ggtttacggc
     2281 agccttgctc ccgcaggcgg gttttacacc ctttactggg tggataactc cgaactgaaa
     2341 aatacggcgc tggagtacca cgaaagcagg ccggtggttg ccattgtcat gatcgacaat
     2401 tatgatgagc tgatgcaaaa tgccaaagaa ggcgaaaagg cggatatact tagccgggtc
     2461 gaaaagcagc tgatggcgtg ggtctccaca accacaggct ttttgtggca ttcggacagg
     2521 gataaataca tttttgtttt tgaagagcgg cattacagaa aaattgttga ggagcgtttt
     2581 gacatactcg acaaggtgcg gcagattatc gcgggtgagc gcatgccggc gacgctttcc
     2641 atcggtgtcg gccgaggcgg aaaatctctt gccgaaaatg aagagatggc ccgtcaggcg
     2701 cttgatatgg ctcttggacg cggcggcgac cagtcggcag tcaagacgcg ggacggatat
     2761 gaattctatg gcggtatctc caaggcggtg gaaaaacgca ccaaggtaaa aacgcggatt
     2821 atcgcgtctg cgctggcgga gctgattgaa ggcagtgaaa atgtgctgat catgggtcac
     2881 aaaaactccg atctcgatgt ggtgggcgcc tgcgtgggca tttaccggtc ggtggtttcc
     2941 agaggtatct ttgctaaaat tgtgattaac cgaagaagca gcatggccgc ggagtttatc
     3001 tcggagcttg aaggccgggg ctacggtgag gcgtttattg acccggagga ttcactgttg
     3061 ctggtgtcga aaaaaacgct gctgattatc gtggatactc accgcaaggc atttgtggat
     3121 tcaccggaac tctatcagaa cgtcaaaacc gttgtggtga tcgatcatca caggaaaatg
     3181 gttgattata ttgataacgc ggttattttt catcatgagc cgtatgcttc atccgcgtct
     3241 gaaatggtct ctgagctggt tcagtatctt gccgattcca gccttacctc ggttgaagcg
     3301 gacgcgatgc ttgcggggat aacgcttgat acccggaatt tcaccgtccg gacaggggtt
     3361 cggacctttg aggccgccgc ctatctgaga agaaaaggcg cagacacggt tcgggtcaag
     3421 cagctttttg cgggcagtat ggaaaattac aggaaaaggt caaaacttgt gtcatccgct
     3481 gagatttacc acggatgcgc aatatcgcga tatgacggcg atcctgacga cagcctgaaa
     3541 ttcgtgacgc cccaggcggc ggacgaactt ttaaccatca ccggcgtcag cgcgtctatg
     3601 gtggcgtatc ccgccggcag cggcgtggca atttcagcaa gatccatggg ggagattaac
     3661 gttcagctga ttatggaaac gctgggcggc ggaggccacc tgaccatggc gggcgcccag
     3721 atggacaaca ttacgatggc ggaggcggtt caccgcatca aagaggctgt cgaccgttat
     3781 tttgccgaaa atggataatt ggaaagccaa ccgcttttct gccccgcgcg gcaggaaatg
     3841 atgcggtgga tgatcaataa cttacgcgcg gagaaacggc ggattgaaaa tgaaggttat
     3901 tttgaaagca gatttaaagg gaaccggcaa agcgggcgaa ttgatcaatg tttccgacgg
     3961 gtacgcaaaa aattatctgc tgcccagggg catggcaatc caggccgaca gcaaagccat
     4021 caacgagctg cgcggaaaag aggagtctca gaagcacagg caggagatgg aaaaggaagc
     4081 ggcggaaaaa atggcggata agctcaaaga cgccgtggtt aaagtttttg caaaagcagg
     4141 ccagaatggg aaactgttcg gggcggtaac gtccaaggaa atcgctcagg ctgtgaaaca
     4201 gcagtttgat tttaatatag acaagcggaa aatcgtactc gacggggata tcaaagcttt
     4261 tggaacctat acgactgaaa tcaagctctt cagtggtata accacatcga tcagggtagt
     4321 cgtgggggaa aaggaataac cccataatcg gcacggaggg ggagaataat caagtatggc
     4381 gcagggcaaa tcttattatg aaatcggtga ctcgggcttt gacggtctgg cgctgccata
     4441 cagcctggaa gcggagcagt cggttctggg ttcgatcctc attgacccgg cgagcattct
     4501 tcaggtgctc gaatacttaa agcctcaata tttttatctt ccccagcatc gtgaaatatt
     4561 tacggttatg gtgagaatgt tttcggagag ctccgtaatc gacttcgtga ccatgctgga
     4621 cgagttaaag tcacagggag tctatgacga agcaggcggc aaagcctatt taatgcagct
     4681 tgcccaaatg gtcccctcgg tcgccaatat tgaggcttat gccaaaattg ttcaggagaa
     4741 atattttctc agaagcctga ttacggcgtc aagggaaatt atctcttcag cagccgaaga
     4801 agccggggac gcccggatga ttctcgattc tgccgaacag aaaatttacg atatcagaca
     4861 ggataaaggc gccgacgggc tgcggcatat caaagacatt atcatagaaa cctatgacag
     4921 actgcatgcc atcagcaatg atgaagacgg aagatttgtc ggaaccccca ctggtttttc
     4981 agagcttgac aggcttatta ccgggcttaa ccgctccgat ctgattcttt tggcggcccg
     5041 ccccgcaatg ggcaagtcgg cttttgcaat caatattgcg gccaatgttg caaaaagagg
     5101 cgagaaggtc gcctttttta ctcttgaaat gaccagggag caaaacgtgg aacgtatgct
     5161 ttcctccgag tccctgattg aaaacagggc cctgagaacc ggtgaattaa aagcggacga
     5221 ttgggtccgg cttgctcagg cggcggacga gctgtcgaaa tatcagcttt attttgacga
     5281 tacttccggg attacgattc cggagatgaa ggcgaaatgc aggagaataa aaggtctggg
     5341 actggtggtg atcgactacc tgcagcttct ctcatcggga agacgtatcg aaaaccgtgt
     5401 gcaggaggtt tcggaaatga cccggaatct gaaaattatg gcaaaggatc tggatgttcc
     5461 gatcattacg ctgtctcagc tttccagggg caccgaatcc aggcagggcc acaggccgat
     5521 gctttccgat cttcgcgaat ccggttccat cgagcaggac gccgatattg taatgttcct
     5581 ttaccgggaa gattattatg agcagaacga gcagaaccac aatcaggcaa cctgcattgt
     5641 ggccaaaaac cgtcatggag cggttgggga tgtaccgctt cactgggatg gagcccatac
     5701 caaatttacg tcccaggaga aatatcgcga tgaaccttaa agcgtggaaa acgattgaga
     5761 aacatcatat gatggagcag ggcggcgcgg tgttggcagg cgtttcgggc ggagccgact
     5821 cagtcgcact gctccacttg ctttgttctt tgcggggccc tcttggcatc cgggtatatg
     5881 cggcccatat taatcatatg ataagggggg ccgaggccct gagggacgag caatatgttg
     5941 aaaagatctg ccgtgagtgg ggtgtcgagc tatttgtcat ccgggcagat gtccggcgtg
     6001 aagccgccga atccggcgag agtcttgaag aggcgggacg gcggatccga tatgattatt
     6061 ttgaaaaaaa aagcctgtga gctgggctcc ggaattgcca cagcccatac gctgagcgat
     6121 agtatggaaa ccgttttgat taatctcgcc cggggaaccg ggcttaaagg tctttgtggg
     6181 attcccccgg tgaggggaaa gatcataagg ccgttgatcg agtgcacaag gcaggagata
     6241 gtcgcctact gccatgagca tcggctcgaa tatgtagttg acagcaccaa cctttccaga
     6301 gattacgtca gaaaccagct caggctggat gttatcccga tcttttaccg gattaacccc
     6361 gcttttgacc gggaaattgc cagaatgacc cgtcagcttg gggaagacag cgattatctg
     6421 agcagcgagg ccgaaaaagc actgaaaaat gcgtcgctcg gggattcttc ctatcgggcg
     6481 gaaacccttc tccgtctgcc gggaccgatt gtttccaggg ttcttctgct tgcggtaaaa
     6541 gaattttcag gcatcggc
//
LOCUS       DATALY010000260         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_831156,
            whole genome shotgun sequence.
ACCESSION   DATALY010000260 DATALY010000000
VERSION     DATALY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831156"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1750
                     /gene="dnaX"
                     /locus_tag="VHR42_07125"
     CDS             80..1750
                     /gene="dnaX"
                     /locus_tag="VHR42_07125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX3026982.1"
                     /translation="MYQALYRKWRPKVFEDVAGQPRITAALRNEVAANRVAHAYLFTG
                     SRGTGKTTCAKIMAKAVNCLRPVNGDPCNECENCRGIESATLLDVVEIDAASNNGVDN
                     IRDLREETSFTPVSAKYRVYIIDEAHMLSTGAFNALLKTLEEPPSYVIFILATTEVHK
                     IPATILSRCQRFDFRRIPADDIAARLQYVAGQEKLTLEPQAASLIARLSDGGLRDALS
                     LLDQCAAGEYQSITEESVYQAAGLTDREYLFELSEAIGSADPARALRTLDRLHAASKD
                     MERLCEELILQFRNVMVLQSVRDADGLINCPPEEEAKLSSLAGMFHAEAVLHILDTLQ
                     MTLDVLRRSPSRRVEMEMCLLRLCRPELDQSTSALLRRLSAMEEKLSSLTAVRAGGAK
                     TVNQPAQTEKLPVPPAKTISSDTITASAPILAPVNPDKTPEQPAETAAPENPEVLQED
                     IRLESWPDILKNLASSDPPLCGVLNGSGAIVRGNHVLVDASNSMFSSLIRQQTHQKAL
                     VEAIRSITGQPYKVGIFKKSLKEQPVSNDPMDEILKNAGTFGIEVKEK"
     gene            1795..2142
                     /locus_tag="VHR42_07130"
     CDS             1795..2142
                     /locus_tag="VHR42_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX3026983.1"
                     /translation="MKARLPQGYGGGGGNMNNMIRQAQKMQEDMAKLQAELDEKEYQV
                     TSGGGAVSLTITGKREVKALTIQPEVVDPDDIEMLQDLLVAAFNEGVRKVDETSSNEM
                     NKLTAGMNMPGMI"
     gene            2220..>2818
                     /gene="recR"
                     /locus_tag="VHR42_07135"
     CDS             2220..>2818
                     /gene="recR"
                     /locus_tag="VHR42_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX3026984.1"
                     /translation="MSYFAAPLSKLIEQFERLPGIGRKTAQRLAFYLLSQPEDQAKEF
                     AQAILEAKTSMHYCKVCQNLTDGEICKICSNPARNQSLICVVEDSRDIVAFERTRDYD
                     GLYHVLHGVISPMDGIGPEHLKIKELLNRVNNNQVEEVIMATNPDVEGEATAMYLSKL
                     LKPLGVRVTRIAYGIPVGGDLEYADEITLLRALEGRSEL"
BASE COUNT          745 a          691 c          733 g          649 t
ORIGIN      
        1 cgcgggatgg gtccggggcc tgcatgtccg gtaacattat ccttttcgaa tgccggtcgt
       61 ttgagtgtgg gaggatatca tgtatcaggc gctttaccga aagtggaggc ccaaagtttt
      121 tgaagacgtc gcagggcagc cccgtatcac cgcagcgctg agaaatgaag tcgccgcgaa
      181 cagggtcgcg catgcctatc tttttaccgg ctcacgagga accggcaaaa caacatgcgc
      241 taaaatcatg gccaaagcag tgaattgtct gcggcccgtc aatggcgatc cctgcaacga
      301 atgtgaaaac tgcagaggga ttgaatccgc aactctgctt gatgttgttg aaattgacgc
      361 agccagtaat aacggtgtgg acaacatcag ggatttgcgg gaagaaacct catttactcc
      421 cgtttcggca aagtatcggg tttatattat cgacgaggcc cacatgctct caaccggagc
      481 ctttaacgca ctgctcaaaa cacttgaaga gcccccgtcc tatgtgattt ttatccttgc
      541 gaccaccgag gttcataaaa ttccggccac cattttgtcc cgctgtcaac ggtttgattt
      601 tcgcagaatt ccagctgacg atattgctgc aaggcttcag tatgtcgctg ggcaggaaaa
      661 actaacgctt gagccgcagg ccgcctccct gatcgcccgt ttatccgacg gcggcctgcg
      721 cgacgcgctg tccctattgg atcaatgcgc cgctggggaa tatcagtcta ttacggaaga
      781 atccgtttat caggcggccg gtctgaccga tcgtgaatat ttatttgagc tgtcggaggc
      841 aatcggcagc gcagacccgg cccgtgcgct caggacactc gaccggcttc atgctgcgtc
      901 aaaggatatg gaacgcctct gtgaagagct tattctccaa ttccggaatg tgatggttct
      961 ccagtctgtc cgagatgcgg atggactgat taattgtccc cctgaggaag aagcaaaact
     1021 ttccagtttg gcgggaatgt ttcatgctga agctgttttg catattctcg atacgctgca
     1081 aatgacgctg gatgtccttc gccgcagccc gtcgcggcga gttgaaatgg aaatgtgttt
     1141 gctgcggctc tgccggccgg agctggatca gtcaacgtcg gcactgctca gacgtctgtc
     1201 tgctatggaa gaaaagcttt catccctcac agccgtacgg gccggcggcg caaaaaccgt
     1261 aaaccaaccg gcacaaaccg agaaattacc cgtccctccg gctaaaacaa tatcttctga
     1321 tacgataacg gcatccgccc ccatcctcgc tccggtcaat ccggataaaa cgccggaaca
     1381 gcccgcagag acagctgctc cggaaaatcc agaagttctg caggaagata ttcggctgga
     1441 aagctggccg gacattctga aaaatcttgc atcttccgat ccgcccttat gcggtgtgtt
     1501 aaacggctca ggggccattg taagaggaaa tcacgtttta gtcgacgcgt ccaattccat
     1561 gttttccagt ctgatccgac agcagacgca tcagaaggca ttggtggaag cgatccggtc
     1621 cataacgggt cagccctata aagtaggaat ttttaaaaaa agtctgaaag agcagcctgt
     1681 ttccaatgat ccaatggatg aaattttaaa gaatgccgga acatttggaa ttgaagtcaa
     1741 agaaaaataa ctacacgtat taataatctt gatattaacc aggaggattt tgacatgaaa
     1801 gcaagactgc cacagggtta cggcggaggc ggcggtaata tgaacaatat gattcgccag
     1861 gctcaaaaaa tgcaggaaga tatggcgaaa cttcaggccg aactggatga aaaggaatat
     1921 caggtgactt ccggcggcgg agccgtttcg cttacgataa ccggcaaacg cgaagtaaag
     1981 gccctcacga tacagccgga agtagtcgac cctgatgata ttgaaatgct tcaggatctt
     2041 ctggtcgcgg catttaatga aggcgtcaga aaagtggatg aaacctcatc caatgaaatg
     2101 aataaactca cggcaggtat gaacatgccc ggaatgatct gattctgtct attcgggccg
     2161 ccgcgtaagc gtcggcaaaa atgatacttt aagccattcc ggctttgggg agggaaccga
     2221 tgtcatactt tgccgcgcca ttgtcgaaat tgattgaaca atttgaacgg ttgccgggca
     2281 ttgggagaaa aacagcccaa agactggcgt tttatctcct cagtcagcct gaggatcagg
     2341 ccaaggaatt tgcgcaggcc atcctggaag caaaaacgag tatgcattac tgcaaggttt
     2401 gccaaaatct taccgacgga gaaatctgta aaatctgctc aaaccctgca cgcaatcaat
     2461 ctctgatttg cgtggtggaa gattcacgcg acatcgtggc ctttgaacgg acccgcgatt
     2521 acgacggcct ttatcatgtg cttcatggag tcatttcacc gatggacggt atcgggccgg
     2581 agcatctaaa aattaaagaa ctgctaaacc gggtcaacaa caatcaggtt gaagaagtga
     2641 ttatggctac caatcctgat gtggaaggag aggcgacagc catgtatctc tctaagcttc
     2701 tcaagccgct gggtgtcagg gtcacccgaa tcgcttacgg aatccctgtt ggcggtgatc
     2761 tggaatatgc cgatgaaatc acgctcctcc gggcactgga aggccgaagt gaacttta
//
LOCUS       DATALY010000261         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_661759,
            whole genome shotgun sequence.
ACCESSION   DATALY010000261 DATALY010000000
VERSION     DATALY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661759"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..723)
                     /locus_tag="VHR42_07140"
     CDS             complement(58..723)
                     /locus_tag="VHR42_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEX3026985.1"
                     /translation="MERTDFKRLPRHIGVIPDGNRRWAVKNGYEKQDGYQYGIQPGFD
                     LYQMCLRLGIGELTFYGFTVDNTKRPSAQTQAFQKACVDAVLQLANRDADLLIVGNTE
                     SPLFPPELLPYANRVKFGRGLIKINFLVNYGWNWDLNGALQNGKIRRCGELTEGIASS
                     DISRIDLVIRWGGRRRLSGFLPVQTVYSDFFVLDELWPDFQPEQFYRALEWYQGQDVT
                     LGG"
     gene            977..1264
                     /locus_tag="VHR42_07145"
     CDS             977..1264
                     /locus_tag="VHR42_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HEX3026986.1"
                     /translation="MLCPCGFDCRKCKEYKKSCAGCREIKGKVYWAEYLGTDVCPIFQ
                     CCVNEKNYRNCGDCPDLPCHLYYDTQDPSQTKEEHEAGVLEQVRNLKSKKE"
     gene            1384..1944
                     /locus_tag="VHR42_07150"
     CDS             1384..1944
                     /locus_tag="VHR42_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3026987.1"
                     /translation="MERKKIYLTTEKELRTYMHPLRQRILFTLERSKDGMTAKQLADA
                     LAIVPSSAGHHLSELEEAGLVELAYTEKVHGFTAKFYKATNVSVSLEKLPPEAASARR
                     AVLQNHINENFGHWADAVEALDESGVARTADAADFSTGMLFLSRQDTLALHKMIRDFC
                     DSHAQPVNGTAPYEFTLFYVRTGDEK"
     gene            1972..>2272
                     /locus_tag="VHR42_07155"
     CDS             1972..>2272
                     /locus_tag="VHR42_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3026988.1"
                     /translation="MKSRIKEYFTAYNGLPKEIYLLFCARVITCLGSFILPLRTLILS
                     QKLGMSESATGNFSAFLIMSQGPCLLLGGRLVDKFGEKKVLVLGQVLGSFFYLMCG"
BASE COUNT          610 a          567 c          528 g          567 t
ORIGIN      
        1 aaagcagcga tttgccgcag tctgtttagc atgtttgcag cctgctcgaa aaagaggtta
       61 accccctagc gttacatcct gcccctgata ccactccaaa gcacgataga actgttccgg
      121 ctgaaaatcc ggccacagct cgtccagaac gaaaaaatca gagtagaccg tctgtaccgg
      181 cagaaagccg ctcagcctgc gccttccgcc ccagcggatc acaaggtcaa tccgtgatat
      241 atcggaggaa gcgattccct cggtcagctc gccgcagcgg cgaattttgc cgttctgcag
      301 cgcgccgttc aagtcccagt tccagccgta attcaccaga aagttgattt tgatcagccc
      361 ccgcccgaac ttcacgcggt tggcatacgg aaggagctcc gggggaaaca gcggcgattc
      421 cgtattcccc acaatcagaa ggtctgcgtc gcggttcgcc agctgaagca ccgcatccac
      481 gcaggccttt tgaaatgcct gtgtctgcgc ggacggccgc ttagtattat ccacggtaaa
      541 gccgtaaaac gtaagctcgc cgattcccag ccgcaggcac atctgataaa ggtcaaagcc
      601 cggctgtatt ccatattgat aaccgtcctg cttttcatat ccgtttttca ccgcccacct
      661 gcggttcccg tccgggatta cgccgatatg ccggggcagc cgtttaaaat ctgttctttc
      721 catttttatc tccacttcaa atagaaatcc ggcaagtccg ccgcacttta gtgtgcatcc
      781 acagatggca tattatacca cttttcttta tccttgattt ttattcttct gttgcaatta
      841 aattcaattt gctttatgat tacataagaa ctatagcagt actttgatgt gtttatccaa
      901 aaaagcggac tgcaaacgcc ataatcaatg aactaaaaaa atatacttta taattaaacc
      961 tcaggaggca aatcatatgc tgtgcccatg cggttttgac tgccgtaaat gtaaagaata
     1021 taaaaaatcc tgtgccggat gcagagaaat taaaggcaaa gtctactggg cggaatatct
     1081 gggaacagac gtctgcccga tctttcaatg ctgcgtaaac gaaaaaaatt accgtaactg
     1141 cggcgactgt cccgacctgc cgtgccactt atactacgat acgcaggacc cttcccaaac
     1201 gaaggaggaa cacgaagcag gcgtactgga gcaggtcaga aatttaaaga gcaaaaaaga
     1261 atagtaaata aaaagtgttt tctctcagga gaacatcttt ttcccttatt tttcttgaca
     1321 taacaatact tgttatgata tagtctttaa cataacaaat tttgttatgt tggaggcgtg
     1381 gtattggaac gaaagaagat ctaccttaca accgaaaaag aactgcgaac ctatatgcat
     1441 cccttacgtc agcggattct gtttacactg gagcgcagta aagacggaat gacggcaaaa
     1501 cagctggcag acgcactcgc aatcgtgccg tccagcgcag ggcaccacct ttccgaactt
     1561 gaggaggccg gccttgtgga gcttgcgtac acagagaagg ttcacggctt cacggcaaaa
     1621 ttctacaagg cgacaaatgt gagcgtcagt ttggaaaagc tgcctccgga ggcggcgagc
     1681 gcccgacgcg cggtgctgca gaaccatata aatgaaaatt tcggtcattg ggccgatgcc
     1741 gtcgaagcat tggacgaatc cggagtcgcc cgcaccgcgg atgccgccga tttttcaacc
     1801 ggtatgcttt ttttgagcag gcaggatacg ctggcactgc acaaaatgat ccgtgatttt
     1861 tgcgattccc acgcacagcc tgtgaatgga acggccccct acgaattcac attattttat
     1921 gtccgtaccg gagatgaaaa atagaaaaca tattctaagt caggaggagc tatgaaatcc
     1981 agaatcaaag aatattttac agcctataac ggtttgccaa aggaaatcta tctattattc
     2041 tgcgcgcggg taatcacctg cctaggcagc tttatccttc cgcttcgtac gcttattctt
     2101 tcgcagaagc tgggtatgtc tgagagcgca acaggaaatt tttcggcttt cttaataatg
     2161 tctcaggggc cctgcctgct tttgggcggc aggctggttg acaaattcgg cgagaaaaag
     2221 gtattggttt taggccaggt tctggggtcc tttttctacc ttatgtgcgg at
//
LOCUS       DATALY010000262         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_661900,
            whole genome shotgun sequence.
ACCESSION   DATALY010000262 DATALY010000000
VERSION     DATALY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661900"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VHR42_07160"
     CDS             complement(<1..280)
                     /locus_tag="VHR42_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX3026989.1"
                     /translation="MNFNIAVIRGDGIGPEIVGEAVKVLEKTGRKFGHTFRFTDVLMG
                     GCAIDATGVPLPDETVRICRESDSVLLGAVGGPKWDTLPGNLRPEKGLL"
     gene            complement(292..783)
                     /gene="leuD"
                     /locus_tag="VHR42_07165"
     CDS             complement(292..783)
                     /gene="leuD"
                     /locus_tag="VHR42_07165"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3026990.1"
                     /translation="MKANGKAIKYGDNIDTDVIIPARYLNTSDPKELAVHCMEDIDKD
                     FVKKVKQGDIIVGGKNFGCGSSREHAPIAIKESGISCVIATTFARIFYRNAINIGLPI
                     LECREASEKIGDGDEVEIDFDSGIIYNKTKNEKYESIPFPAFIQGIIHSNGLMNAIKN
                     NKI"
     gene            complement(797..>2032)
                     /gene="leuC"
                     /locus_tag="VHR42_07170"
     CDS             complement(797..>2032)
                     /gene="leuC"
                     /locus_tag="VHR42_07170"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX3026991.1"
                     /translation="ILAAHARLPKVFPGQLIEAQLDLVLGNDITTPVAIREFDKTGVT
                     EVFDKNKVALVMDHFTPNKDIKAAEQCKTVREFAKMYNIVHFFDVGDMGIEHALLPEK
                     GLVVPGDFVIGADSHTCTYGALGAFSTGVGSTDMAAGMAKGICWLKVPSAIRFVLHGK
                     PQKWVSGKDIILHIIGKIGVDGALYKSMEFTGDGLRSLSMDDRLCMANMAIEAGAKNG
                     IFEVDDITLNYVKSHSEKSFSVYKADEDADYDATYNIDLSEIRPTVAFPHLPDNTKTI
                     DECSDIPIDQVVIGSCTNGRLEDLRAAAAVLKNHKVAPDVRCIVIPATQKIYLDAVEE
                     GLISVFIKAGCAVSTPTCGPCLGGHMGILAKGERAVATTNRNFIGRMGHTESEVYLAS
                     PAVAAASAVKGKITDPESI"
BASE COUNT          467 a          535 c          486 g          544 t
ORIGIN      
        1 caagcagccc cttttccggg cggaggttcc ccggcagcgt atcccatttg ggcccgccga
       61 ccgcaccgag caaaacgctg tcgctttccc ggcagattct gaccgtttca tcaggaagcg
      121 gaacgcccgt tgcgtcaatg gcgcatccgc ccatcagcac atcggtgaac cggaaggtgt
      181 gcccgaattt ccggcccgtt ttttcaagta ccttgacggc ttcccctacg atttcaggac
      241 cgataccgtc gcccctgatt accgctatat tgaagttcat gaaaatgccc cctaaatctt
      301 attatttttg attgcgttca tcagcccgtt tgaatggata ataccctgaa taaacgcagg
      361 gaacgggatg ctttcatact tttcgttctt tgttttgtta tagataatcc cgctgtcaaa
      421 atcgatttcc acttcgtcgc cgtccccgat tttttcggac gcttccctgc attccagaat
      481 cgggagcccg atattgatgg cgttgcggta aaaaatccgg gcaaaagtag tggcgatcac
      541 gcaggatatg cccgattcct taattgcgat cggagcgtgt tctctcgatg agccgcagcc
      601 gaaatttttg ccccctacga tgatatcccc ttgctttacc tttttgacaa aatccttatc
      661 aatatcctcc atacaatgga cagcaagttc tttcggatcg gaggtgttca gatatctcgc
      721 cgggatgatc acatcggtgt cgatattgtc accatactta attgcttttc cgttcgcttt
      781 catcctcatc actccttcaa atggattccg gatcggttat ttttcctttt acggcagagg
      841 cggccgcgac agcggggctg gcaagataga cttctgattc agtgtgcccc attctaccaa
      901 tgaaattcct gtttgtggtc gcaacggcgc gctcgccttt cgcgaggatt cccatatgac
      961 cgccgaggca cgggccgcag gttggggttg aaacggcgca gcccgctttt ataaagacag
     1021 aaattaaacc ttcctcaact gcgtcaagat agatcttttg tgttgccggg atcacaatac
     1081 agcgcacgtc gggcgctact ttatgattct ttaaaaccgc agctgccgcg cgcagatcct
     1141 caagcctgcc gtttgtgcag gaaccgatga caacctgatc gatcgggatg tcactgcatt
     1201 cgtcgattgt tttggtgttg tcgggcaggt ggggaaaggc aacggtcgga cggatttctg
     1261 aaaggtctat attataggtc gcgtcataat cagcatcttc atccgcctta tagacagaaa
     1321 aggacttttc ggaatgactt ttaacataat tcagtgtgat gtcgtcaact tcgaatattc
     1381 cgtttttcgc acccgcttca atcgccatat tggccataca aaggcggtca tccatcgaaa
     1441 gggagcgaag cccatctccg gtaaactcca ttgacttgta aagcgcgccg tctacgccga
     1501 tttttccgat aatatgaaga ataatgtcct tgccggaaac ccatttctgc ggttttccgt
     1561 gaagaacaaa acggattgcg gagggaacct tgagccagca gatgcctttt gccattccgg
     1621 ctgccatatc ggtgctgcct acccctgttg agaaagcgcc gagcgcgccg taagtacagg
     1681 tgtgggaatc ggctccgata acaaaatcgc ctggcacaac aagccctttt tcaggcagga
     1741 gggcatgctc gatgcccata tcgccgacat caaagaaatg gacgatgtta tacatctttg
     1801 caaattcgcg aacagttttg cattgctctg cggctttgat atctttattc ggcgtaaaat
     1861 gatccatgac cagtgcaact ttgtttttgt caaaaacctc tgtgacgcct gttttatcga
     1921 attcacggat agcgacagga gtcgtgatgt cgttaccgag aacaaggtcg agctgcgcct
     1981 ctatcagctg cccgggaaac actttcggca gccgggcatg tgcagccaga at
//
LOCUS       DATALY010000263         8116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_143810,
            whole genome shotgun sequence.
ACCESSION   DATALY010000263 DATALY010000000
VERSION     DATALY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143810"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VHR42_07175"
     CDS             <1..459
                     /locus_tag="VHR42_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3026992.1"
                     /translation="KLFVSFNLQDNLLFVALFIATTNLPYGIWMSKNFMDSIPVPLEE
                     AAKVDGADSTQIMRLIIMPLMKPGIFTVLIYVFSDVWGTFFVPFILLQTPSKFPAAVR
                     IYQFFQQSEFIEYGNLAAYSMVYMIPCVILYAFAQKYMSQGFAMSGAEKG"
     gene            510..1706
                     /locus_tag="VHR42_07180"
     CDS             510..1706
                     /locus_tag="VHR42_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026993.1"
                     /translation="MKRSMENGIADIPFYQGGGPRDLFSCNNGCQMLRVTGTARLEYE
                     AYIATLENAGFVLYSKHAIKENLFATYQGKSVIIQANYTDCDHISRIIADPNTILYKR
                     EEDTVSPKRCDTTLYQMELDYRTIDCGMCYILQCKDGSFFLIDSAHMNSTNDHKRLYD
                     LLCRLVPEGQKIVIAGWFFSHAHQDHIVKFMDFIRSGFDNYEIKCLYFNFPELNVPGS
                     EEWSASDKQTMLEFDELIKSHNELPVVKLHTGQHFFVCNLEFEVLSTHEDIYPGSLAC
                     FNDSSTVLLMTAEGCKTIFLGDANFTECNILIARYGDYLKSQIVQVAHHGYNSDNIEI
                     YRHIAAQVALYPTSPSRYEDTKDSRSNQLIRELTKEIYLSGNGTTALRLPYQNHSAVT
                     FPKEIF"
     gene            complement(1791..2291)
                     /locus_tag="VHR42_07185"
     CDS             complement(1791..2291)
                     /locus_tag="VHR42_07185"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3026994.1"
                     /translation="MDYDKAASYWEEKSVSSVKMPRDALRAKMEQFIEGHNTCALATG
                     SGDFVRCTPIEYSYRGGMLWMFSEGGLKFLALKSNKNVCVSIFEPYAGFGKLAGMQIS
                     GTARMVEPLSREYLDLLELKKIPVESLAKLPHELNLICITPVRIDFLWSELKKMGYDS
                     RQSLTF"
     gene            2586..3872
                     /locus_tag="VHR42_07190"
     CDS             2586..3872
                     /locus_tag="VHR42_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026995.1"
                     /translation="MDQNGLKWFEKKWFVNLKIKKRLTVSFLFLAAIAAAIGIAGIVF
                     SLSAHVPNAGIFAGIIGAASLFEILAALLLGSINAFLITDPTEKNERVIGKFSVGDFN
                     TGDIIRKRDWITAEYQDEIGHFSRRIKDIMAYLRGVDTCIKTVAAGDLTAEVPVCSAE
                     DKIGNNLSHMVDNFHKLIGAIVAAADQVNMGARLVSDSSQTLAQGASEQASTVEQLTA
                     SLSQISEHTRLTAESAKKADALAQTAKANASQGNDQMKDMLNAMEEINRSSANISNVI
                     KVIQDIAFQTNILALNAAVEAARAGEQGKGFAVVADEVRNLASKSAEAAKETSELIEG
                     SIKNVEAGTELANRTAESLEEIVSQVGQAADFINSIAAASEEQAHSLEQIHTGIDQVS
                     QVTQATAATAQESAAASEELSGHAVQLKTQVGAFTI"
     gene            4641..5912
                     /locus_tag="VHR42_07195"
     CDS             4641..5912
                     /locus_tag="VHR42_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3026996.1"
                     /translation="MKWFERKWFVNLKIKTRLTLSFLLVGVLAAAIGVAAILFSLRAN
                     IPNAVPFSVIIGVLSLLVIAAALLLGPINAFLVTDPMYKNERIMQKFSVGNFYTRDII
                     RKRDFATAQYQDEIGRFSRKMQGVMEYLRNLDACIKKVAKGELTTEVPLCSPQDEIGS
                     SLAQLVENFHGLVTTITEIVEQVNSGAKLVSDSSQTLAQGASEQASTVEQLTASLSQI
                     SEHTRLTAESAKKADALTQTAKANASQGNDQMKDMLNAMEEINRSSANISNVIKVIQD
                     IAFQTNILALNAAVEAARAGEQGKGFAVVADEVRNLASKSAEAAKETSVLIEGSIEKV
                     TAGTDIANRTAASLNEVVSQVEQAANFINSIAVASEEQAHSLEQIYTGIDQVSQVTQA
                     TAATAQESAAASEELSGHAVQLKTQVGAFTI"
     gene            6142..6342
                     /locus_tag="VHR42_07200"
     CDS             6142..6342
                     /locus_tag="VHR42_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3026997.1"
                     /translation="MKNRKMKIARVECDMSQEDLANAVGVTRQTIGLIEGGRYNPSLN
                     LCIAICRATGKTLNDLFWEDEL"
     gene            6339..6902
                     /locus_tag="VHR42_07205"
     CDS             6339..6902
                     /locus_tag="VHR42_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3026998.1"
                     /translation="MKTMEFKQQLSQNIVRRLKQDERTVKNTEAACVRAVLVTYLFLT
                     VEIVVKAARKETCGWEVALILLISAVLLLARKDRTEILLPRTLSRKLLPTGTTAKAKH
                     TRLKQYFMNSCAYAAIFSVLELLYSSGLNVKTLAGISPISTIVTFLFLFVLFLAISFL
                     WGEHMVKRYNRICSKLEEESESEDGSE"
     gene            complement(6921..7976)
                     /locus_tag="VHR42_07210"
     CDS             complement(6921..7976)
                     /locus_tag="VHR42_07210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3026999.1"
                     /translation="MRKHYLDNIRWGTVLLVLVYHVFYLFNGVGVLGGVGSFSKVQVQ
                     DAVLYFIYPWFMVLLFLVAGISSRYALEHCSIKQFIKDRTVKLLVPSTLGLFVFQWMV
                     GYFNLKINGVLDATPAFLRYPIMVLTGTGPLWFIQMLWLFSLLLVLIRKLDSKDGFYT
                     LCGKCSLFVILLLFLPIWGASQILNMPVVTTYRFGIYFMAFLLGYFVFSHDQTQDKVR
                     KIHLPMLILAVVLGIAYTTYYFGRNYADTACLQSIFTNLYLWVAILAILGCGKAWFDK
                     TSKFAAYMTKSSFGIYIVHYLVVLAACCYLKFDTNLPVFAIYLMAIFSVLILSPVLYE
                     LLRRIPILRYLVLGIKG"
     gene            complement(7979..>8116)
                     /locus_tag="VHR42_07215"
     CDS             complement(7979..>8116)
                     /locus_tag="VHR42_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX3027000.1"
                     /translation="ARDLFHIKPGDTLILLADVKKGIAIPPKESFAELAAKIFGDKVE
                     S"
BASE COUNT         2249 a         1951 c         1918 g         1998 t
ORIGIN      
        1 aagttgtttg tttcgttcaa tttacaggat aatctgctgt tcgtcgcttt gtttatcgca
       61 acaacgaacc ttccttacgg aatatggatg tcgaaaaatt ttatggattc cattcccgtg
      121 ccgctggagg aggctgctaa agtagacggc gccgattcca cacaaattat gcggctgatt
      181 attatgccac tgatgaagcc cggtattttc accgtgctga tttatgtgtt ttccgacgtc
      241 tgggggacct tcttcgttcc atttatctta ctgcaaacac cttccaaatt tccggcagca
      301 gtccgcattt accagttttt ccagcaaagc gaatttattg aatatggaaa tctggccgcc
      361 tattccatgg tctacatgat cccgtgtgtt attctgtacg cattcgcgca gaaatacatg
      421 tctcagggct ttgcaatgag cggagcggaa aaagggtaat ctgtgagaat tacggatggg
      481 ttacttttcg tgaaaggatg catgttttta tgaaacgaag tatggaaaat ggtattgccg
      541 acattccttt ttatcagggc ggcgggcccc gcgacctgtt cagctgcaac aacggatgcc
      601 aaatgcttcg cgttaccggc acagcaagac tggaatacga agcatacata gctacccttg
      661 aaaacgcagg ctttgtgctt tacagtaagc atgccataaa agagaatctt tttgcgacct
      721 atcaggggaa atctgtaata attcaggcaa attatactga ttgtgatcat atttcacgaa
      781 ttatagcaga tccgaataca attctctata agcgggaaga agatacggtt tccccgaaac
      841 gatgcgatac aactttatac caaatggaac tggattatcg gacgattgac tgtggcatgt
      901 gttatatcct gcaatgtaaa gacggaagct tcttcctgat tgacagcgca catatgaact
      961 cgaccaatga tcacaagcgt ttatacgatt tactgtgccg ccttgtgccg gaaggacaga
     1021 aaattgtcat agccggatgg ttcttcagtc atgcgcatca ggaccatatc gttaaattta
     1081 tggattttat tcgatcgggc tttgacaatt atgagattaa atgtctttat ttcaattttc
     1141 ccgagctaaa tgtacccgga tcagaggagt ggagcgcatc ggataagcag acgatgctgg
     1201 aatttgatga actgatcaaa agccataatg aacttcctgt ggtaaagctg cataccgggc
     1261 aacatttttt tgtatgcaat ctggaatttg aggtactgtc aacacacgaa gatatctatc
     1321 ctgggagttt ggcttgtttt aatgattctt ccactgtgct gttaatgacg gcagagggct
     1381 gcaaaacgat atttctcgga gacgcaaatt tcaccgaatg caatatttta atcgcgcgct
     1441 acggcgacta tttaaaatca caaattgtac aggttgccca tcacggatac aacagcgata
     1501 atattgaaat ctaccggcat atcgccgctc aggttgcttt atatcctaca agtccgagcc
     1561 gatatgagga tacaaaggat tccaggtcga accaattaat tcgcgaattg accaaggaga
     1621 tttatttgtc aggaaacggt acgacggcgc ttcgtcttcc ttatcaaaat cattcggcag
     1681 ttacgtttcc gaaagaaatt ttttaacgcc gagaaaattc gtaaggcaaa cacaaaggac
     1741 ccggaaatac ggcagatatg ccgctttccg ggtcctttta tctcgttctc tcagaacgta
     1801 agagactgac gggaatcata ccccattttt ttcaattccg accacaggaa atcgattcgg
     1861 acgggagtaa tacagattag gtttaattca tgcgggagtt ttgcgaggct ttcaaccggt
     1921 atttttttca gctccagcag atcaagatac tctcgggaca acggctccac cattcttgct
     1981 gtaccggaaa tctgcatacc agctaatttt ccaaacccgg cgtacggctc aaaaatggag
     2041 acacagacgt ttttgttact cttcagcgca aggaacttta agccgccttc cgaaaacatc
     2101 cagagcattc cgccccgata gctgtactca atcggcgtgc agcggacgaa atcgccggaa
     2161 ccggttgcaa gcgcgcaggt gttgtgccct tcaatgaatt gttccatttt ggcccgcaga
     2221 gcatcccggg gcattttcac cgatgaaacg ctcttttctt cccaatagga agcagccttg
     2281 tcataatcca aaagtaatcc tccttatttt tggcggacgg ctcgccgttc gctgattttc
     2341 ctatgccaac cggcaccgat tcattcctgt tttcatcctt cccctgctga taacaaaaaa
     2401 cgcgttttct ttctaactat ttcaaattat gttaatttgt gtccaatatt agtcgatata
     2461 tatagtaagg cgttcaaatt tttaaaaaat ttatatttat tgatgaaaca tttcaactgc
     2521 aacgattcaa aaaacgaaac cagcagatca agccacgcct aattttcatt gacgaaggaa
     2581 tctctatgga tcagaacgga ttgaaatggt tcgaaaaaaa atggtttgtt aacctaaaaa
     2641 tcaaaaagcg cctgaccgtc agcttcctgt tcctagcggc catcgccgct gccatcggca
     2701 tcgccggtat cgtgttctct ctgtcggcac atgtcccgaa cgcaggcatc tttgcaggaa
     2761 tcatcggcgc agcttcccta ttcgaaattc tcgcggcttt gctgctgggc tcgattaacg
     2821 cttttttaat tacggaccct acggaaaaga acgaacgcgt catcggcaaa ttctccgtcg
     2881 gggacttcaa cactggcgat attataagaa agcgggattg gattaccgcc gaatatcagg
     2941 atgaaatcgg ccacttttcc cgcagaatca aggatataat ggcatacctc cgaggcgtag
     3001 atacctgcat caaaaccgtc gccgccggtg acctaaccgc agaggtgcct gtctgctccg
     3061 ccgaggataa aatcggcaac aatctttccc acatggtaga caatttccat aagctgatcg
     3121 gcgcaatcgt tgccgccgca gaccaggtaa acatgggggc aaggctggtt tcggattcca
     3181 gccagactct ggctcagggc gcttcggagc aggccagcac ggtggagcag cttacagctt
     3241 cgctttccca gatttcggag catacgcgtc tgaccgcaga aagtgctaag aaggccgacg
     3301 ctctcgccca aactgccaaa gcgaatgcct cacagggcaa cgatcagatg aaggatatgc
     3361 taaacgcaat ggaggaaatc aaccgttcct ccgcaaacat cagtaacgtc atcaaagtca
     3421 ttcaggacat cgccttccag accaacattc tggcgctgaa cgctgccgtt gaagctgcgc
     3481 gggccggtga gcagggcaag ggcttcgccg tggtggcgga tgaggtccgg aatctggcca
     3541 gcaaatcggc ggaagccgcc aaggaaacca gcgaactgat cgaaggctct ataaaaaacg
     3601 tggaagcagg aacggagctt gccaaccgca ccgctgagtc actggaggaa atcgtctctc
     3661 aggttgggca ggccgccgat tttatcaact ccattgcggc cgcttccgag gaacaggcgc
     3721 acagtctgga acagattcat accgggattg atcaggtttc tcaggtgact caggcgaccg
     3781 cggccacagc gcaggaaagc gctgccgcga gcgaggagct ttccggtcac gccgttcaac
     3841 tgaaaacaca ggtcggcgca tttacaatat aaaacccgaa ccgatccaaa gatccgatac
     3901 cttcagaccg aaaagcctcc cccgttggct tttcggcctt ttattatggc taatcagccg
     3961 caatgacatg agaacaggca gcaaaaacat cagcgcattc atattttcaa tctagaattc
     4021 tttaaatcca gtatatcacg actttgagaa aacagaagat aaaaaagtcg tcggctccct
     4081 aagattttct cggggattga tcgagcagga gcagttgcat cccacctgaa gaatctgtta
     4141 atagaggaaa tccaaatcgg cgtaaaaagg agcaaagaag aaaccgaatc ttgaaactga
     4201 aacggcttct ggcgtagacg ttctgaaagt ggttatagaa cgtatgcctt gtcagcttgc
     4261 ggccgtctgt aatcatttga acataaatcc gattgatcgg gtagttttca tccgtttcct
     4321 gagcgaatcc aagcgcaatc ttggtaaccg atgagcttga cattcttccg ccgctttccg
     4381 ttcctatagg atgatttttt actatagcat aatttcccgc cgggttggaa gtactgaccc
     4441 agcacagata ggaataatgt ctttctatat tattttttca taatatcatg aattttctat
     4501 tgtgaatttt ttgctaaagt ctgtcgatat ataaatgagt caattttggt ttttcaaatt
     4561 ttcaacatga aaccgcagca aattccatac tttttatcag ggaggtcctt acaataaaca
     4621 cggaagaatt gaatgaagaa gtgaaatggt ttgaaagaaa gtggtttgtc aatctcaaga
     4681 tcaaaacacg ccttacactc agttttctgt tggtcggcgt tcttgccgct gccattggtg
     4741 tcgcggcaat cctgttttcc ctgcgtgcaa acattccgaa tgcggtgccg ttttcggtca
     4801 tcatcggagt gctttccctg cttgtgatag ctgccgctct tctgctcggt cccatcaacg
     4861 catttcttgt cacggaccct atgtataaaa acgaacgcat tatgcaaaaa ttctcggtcg
     4921 gcaatttcta cactcgcgac atcatccgga aaagggattt tgccaccgcc cagtatcagg
     4981 atgaaatcgg cagattttct cgaaaaatgc aaggagttat ggaatacctc aggaacctgg
     5041 acgcctgcat taaaaaagtt gcgaagggtg aactgactac agaggttcca ctctgttccc
     5101 ctcaggacga aatcggcagc agccttgccc agttggtcga aaattttcac ggcctggtca
     5161 ccaccattac cgaaatcgtc gagcaggtta actcgggggc aaaacttgtc tctgattcca
     5221 gccagactct ggcccaggga gcttcggagc aggccagtac ggtggagcag cttacggctt
     5281 cgctttccca gatttcagag catacgcgtt tgactgcgga aagtgctaag aaggccgacg
     5341 ccctcaccca aactgccaaa gcgaatgcct cacagggtaa cgatcagatg aaggatatgc
     5401 tgaacgcaat ggaggaaatc aaccgttcct ctgcaaatat cagcaatgtc atcaaagtca
     5461 ttcaggacat cgctttccag accaatattc tcgctttgaa cgctgctgtt gaagccgcac
     5521 gggccggcga acaagggaaa ggcttcgccg ttgtggcgga cgaggtccga aatctggcca
     5581 gcaaatcggc ggaggccgcc aaggaaacca gcgtactgat cgaaggctcc attgaaaaag
     5641 tgacggctgg aacggatatt gccaaccgca ccgccgcgtc gctgaatgaa gtcgtctctc
     5701 aggtcgagca ggccgctaat tttatcaact ccatcgcggt cgcgtccgaa gaacaggctc
     5761 acagtctgga acagatttat accggaattg atcaggtttc tcaggtgact caggcgaccg
     5821 cggccacagc acaggaaagc gcggcagcca gcgaggagct ttccggccac gctgttcaac
     5881 tgaaaacaca ggtcggcgca tttacaatat aaaacctgaa ccgatccaaa gatccgatac
     5941 cctcaaaccg ataagcctcc ccccgttggc tcatgggttt tatccgggat aacccccatg
     6001 cccaatggag ggaacaaaaa gtgaagcccg ggaagtcggt gttggtgccg cttcccgggc
     6061 ttcttccgct atgaaatgca atatatatat tgcattatgc attctatcgt gtataatgta
     6121 ctccagagga tgtggtttgg attgaaaaac agaaaaatga agattgcgcg ggtggaatgc
     6181 gatatgtctc aggaagacct ggcgaatgca gtcggcgtta ccagacaaac aattggttta
     6241 atcgagggcg gccgctacaa tccttcccta aacctttgca ttgcaatttg ccgcgcgacc
     6301 gggaaaacgc tgaatgatct gttttgggag gatgaattat gaaaacgatg gagtttaagc
     6361 agcaattatc gcaaaatatc gttcgccggc tcaaacagga tgagcgcacg gttaagaata
     6421 ccgaagcggc atgcgtacgg gccgtattgg tgacttatct tttcctcacc gttgagattg
     6481 tcgtcaaggc tgcaagaaaa gaaacctgtg ggtgggaagt tgccctgatt ctactgatca
     6541 gcgccgtatt gctccttgca cgaaaagacc gaacggagat tttgctgccg cggactctgt
     6601 cccgcaagct gctgccaacg ggtacaactg caaaagcaaa gcataccagg ctgaaacagt
     6661 atttcatgaa ttcctgtgcc tacgcggcga tattttctgt gctggagcta ttgtacagca
     6721 gcggtctgaa cgtgaaaacc ctcgcaggaa tttctccgat ttccacgatt gtgacgtttc
     6781 tcttcctgtt tgtattattc ctggcaatca gcttcctatg gggtgaacac atggtcaaac
     6841 ggtataacag aatttgcagt aaacttgagg aggaatccga gagcgaggat ggctcggaat
     6901 aaagaatgct ctgcttgcat tcatcctttg atgcccaaaa ccagataccg caggatcgga
     6961 atccgccgga gcagctcata caaaacgggt gaaaggatca gcactgagaa aatggccatc
     7021 agataaattg caaataccgg cagatttgtg tcgaacttca gataacagca ggctgcaaga
     7081 acgacaagat agtgaacaat gtaaatgcca aagctggatt ttgtcatata agccgcaaat
     7141 ttcgaggttt tgtcgaacca cgccttcccg cagcccaaaa ttgcgagtat cgcgacccac
     7201 aggtacaggt tggtaaaaat gctttgcagg caggcagtgt cggcatagtt ccggccgaaa
     7261 taatatgtcg tgtaggcaat ccccagtaca acggcaagaa tcagcatcgg caagtgaatc
     7321 ttccgcactt tgtcctgtgt ctgatcgtga gaaaacacaa aataaccgag caaaaacgcc
     7381 ataaagtaga tgccaaagcg atacgtcgtg acgacaggca tattgagaat ctgcgacgct
     7441 ccccagatgg gaaggaaaag aagaaggatc acgaaaaggc tgcacttccc gcagagcgta
     7501 tagaatccgt ccttggagtc gagctttcga atcagcacga gcagtaacga gaacagccac
     7561 agcatctgga taaaccagag ggggccggtg ccggttaaca ccatgatcgg ataccgaaga
     7621 aaggccggag tcgcatccag gactccgttg atttttaggt tgaagtaacc gaccatccac
     7681 tggaatacaa aaagccccag ggtagaggga actagcaact ttacggttct gtctttgata
     7741 aactgtttga tactgcaatg ctccagtgcg taccgggagc tgattccggc aaccagaaac
     7801 agaagcacca taaaccacgg gtagataaaa tacaacacag cgtcctgaac ctgcactttt
     7861 gaaaaactgc cgactccgcc gagcaccccg acgccgttaa atagataaaa cacgtgataa
     7921 accagaacca gcaggacagt accccagcgt atattgtcca agtaatgttt tctcacggtc
     7981 agctctccac cttatcccca aaaatttttg ccgcaagttc ggcaaagctt tctttgggcg
     8041 gaatcgcaat gcctttcttg acatcggcaa gcaagatcag cgtgtcgccg ggtttaatat
     8101 ggaacaggtc ccttgc
//
LOCUS       DATALY010000264         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_488366,
            whole genome shotgun sequence.
ACCESSION   DATALY010000264 DATALY010000000
VERSION     DATALY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488366"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /gene="rplT"
                     /locus_tag="VHR42_07220"
     CDS             <1..352
                     /gene="rplT"
                     /locus_tag="VHR42_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX3027001.1"
                     /translation="AKGYWGGKSKLFRTANQAVMKSLSYAYVGRKRKKRDFRALWITR
                     ISAACKMNGMNYSTFINGLKKSGIMLNRKMLAELAVSDQKGFFHSGRKSQSRTLIELK
                     QPGLNPGFCAPCRN"
     gene            complement(579..776)
                     /gene="rpmI"
                     /locus_tag="VHR42_07225"
     CDS             complement(579..776)
                     /gene="rpmI"
                     /locus_tag="VHR42_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX3027002.1"
                     /translation="MPKLKTHSGLSKRIKITKKGKVKRSHAFMSHILTKKDTKRKRGL
                     RKAAYADATNIGAIKHMIPYM"
     gene            complement(801..1328)
                     /gene="infC"
                     /locus_tag="VHR42_07230"
     CDS             complement(801..1328)
                     /gene="infC"
                     /locus_tag="VHR42_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX3027003.1"
                     /translation="MLDISSKELLINEEIRDREIRVIDADGSQMGIMLTRDAMKIALE
                     KNLDLVKIAPQAAPPVCRIMDYGKFRFEQAKKDKEARKNQRIVEVKEIRMSLNIDTHD
                     FETKISHAAKFLQSGDKVKVTVRFRGREMAHTELGSTLLKKFAESFLESANIEKMPKL
                     EGRSMSLFLAPKPVK"
     gene            complement(1777..>5010)
                     /locus_tag="VHR42_07235"
     CDS             complement(1777..>5010)
                     /locus_tag="VHR42_07235"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX3027004.1"
                     /translation="ADEAAHPAFSRLFLHASYREDTKILIFARRPRSGEMPAFLAVGF
                     AEQELDFEFDTDRERLLSRPDGIASLPEALKAEMKSGTGALPEAAAALRITTELPPHG
                     KKNCTLLLAAANSEDEAAARLIEARRHGYSAFIRSAAGKDSNEMETRLAALILPQILF
                     PVKDYVDKREAVANNRLSQQGLWSLGISGDYPLILFEYRGQRDAERLLPYIKTQRALR
                     LKGIQADLVITYREGGDYSRQQHSVLNDMIKSCGSEYLIGGRGGVHLVNLSIHPEEIY
                     YLLLTTACHTATSGVGKKLPDDHYESAALNSGNPVSNRAESEKNLAVYGGEFLGPDFI
                     ISHEQKPSAPWCHVLANRTFGTLLSDRALGFTWALNSRENKLTPWNNDPLADNRGEML
                     LLKVGNKIFDLCANARVTFSPSKAVYETVIDQLFCRVTVEIPGKMMAKLVRLEIENRA
                     KKEYHLRIAYYVEPIMGVNASTRRHISIQQDQGVVQMRNAWAPVRGAGFLTALEGMDG
                     FLYNRAGILSGNWENAGDDISPDSCAAAVVKRKLPPGKTTVATFVLGWAAEKGAAQAM
                     AGMLKSTDPARSAGDGPLSGDGLKVSTPDPYLDALMDTWLRWQFMSSRIMGKTGFYQN
                     GGAWGFRDQLQDCCAALLFDPQFAKAHLYRSAAHQFKEGDVMHWWHQLPPRDGGTRGV
                     RTRCSDDMLWLPLTLCEYLEKTGDDSIFDHEVYYLDGKELEQKEEDRYFFPQRSEEKE
                     NLYFHCVRAIERAKRLGAHEIPLFGGGDWNDGMNLVGIAGKGESVWLAMFLSIVLERF
                     APLCKKQGDETRGEQYLAESAQLRKAVDETCWDGKWYLRGFYDDGFPLGGKESGECRI
                     DLLPQSFSAIAGFPDKERRQAALDSALKMLKDDRLKIVRLFTPPFDGGGRNPGYICSY
                     PPGIRENGGQYTHAAIWLALGLLKEGRGDDAFQILQYINPAYRSLDQESSRTYRLEPY
                     AIAADIYANQSCEGRGGWSLYTGAAGWYYRTVFEDLLGIQIRADHIGLNPVMPSDWQG
                     FSAEIRLRGSEIHLRVEKGTKKKLTVDGAEAESIPLDGKKHNVLLVL"
BASE COUNT         1154 a         1410 c         1214 g         1232 t
ORIGIN      
        1 tgctaagggt tactggggcg gcaagagcaa acttttccgc acagccaacc aggcggtgat
       61 gaaatctctt tcgtacgcat atgtcggacg caagaggaaa aaacgtgact tccgcgcgct
      121 gtggatcacc agaatttccg cggcctgcaa gatgaacgga atgaattatt ccaccttcat
      181 caacggcctg aagaaatccg gaatcatgtt gaacagaaaa atgcttgcgg agcttgccgt
      241 cagcgatcag aaaggttttt tccactctgg tcgaaaaagc caaagccgca ctttaattga
      301 actcaaacag cccgggttaa acccgggctt ttgtgcgccg tgccgcaatt aggtggaaga
      361 tccgggcggc ccggcgagcc ggggggcaga gtatgattac ggaaatatca agcagggaaa
      421 acaggttgat caaagaatac gtccgcctgt ctggaaatgc cgcccttaac tctcgccatt
      481 tctaaatacc tccataccgc ggaaaacggt acaatcttct gatagtctca ggatctcacg
      541 gtcagcccgg gcaacagcca aaagccggtc gaacgggttt acatataggg aatcatatgt
      601 ttaattgcgc cgatatttgt agcatccgca taagcggcct ttctcagacc ccttttgcgt
      661 ttcgtatctt ttttcgtcaa aatatgcgac ataaatgcat gcgagcgttt gactttacct
      721 tttttcgtaa tttttatacg tttggataat ccgctgtgtg ttttcagttt tggcattttg
      781 atttcctccc ctgctaatgg ttatttgacg ggcttaggcg ccagaaacaa agacatactg
      841 cgaccttcga gcttcggcat cttttcgata ttggcgcttt ccagaaagct ctcggcaaac
      901 tttttcaaaa gtgtgcttcc cagctcggtg tgggccatct cgcgtccgcg gaaacgaacc
      961 gtcaccttaa ccttatcgcc actctgaaga aattttgcgg cgtggctgat tttggtttca
     1021 aaatcatgcg tgtcgatgtt gagggacatc cgtatctctt tcacttcaac gatacgctga
     1081 ttctttcttg cttccttatc tttttttgcc tgctcgaagc ggaatttgcc ataatccata
     1141 atacggcaga ccggcggcgc cgcctgcgga gcgatcttga ccaggtcaag atttttctcc
     1201 agggcaatct tcatcgcgtc tcttgtcagc atgataccca tctgactgcc gtctgcatca
     1261 ataacccgta tctctctgtc tctaatctcc tcattaatga gcagttcctt actgctaata
     1321 tccagcacct ccaaatagcc caaataaaat acaaaacgcg gatagaactt tccatccgcg
     1381 ttacaacacc tgatcaatcg gagccgcaaa aaacggctgc cgtttttggt attgaccccg
     1441 cggctgagca gcgcacgagg tgaggatgga taacctctcc tgctttgttg tcaaagataa
     1501 tcataacagc aattctgttt taagtcaagg gaactaaaaa cttttcttgg tttttctttg
     1561 taaaataaca gttcatgcgg ctgaaccgcg gcccgccggc aaacagaaaa gctgccggtc
     1621 accgggaata agataacaca aaccccgctg tttcggcaat ccctgattca atatctttaa
     1681 aaatcggaac gccgggtcga tggttctttt tcgctggtgc aaaggcatac cattcacaaa
     1741 gtttagccgt cccgagaact caggacggct aaacagctat aaaacaagaa ggacattatg
     1801 cttctttccg tcaaggggta tgctttctgc ttccgctcca tccaccgtca gctttttttt
     1861 cgtacccttt tccacgcgta aatgaatctc gcttccgcgc agtctgattt ccgcggaaaa
     1921 gccctgccag tccgagggca tcacggggtt aaggccaata tggtcggccc ggatttgaat
     1981 cccaaggaga tcttcaaaca ccgttcggta ataccatccc gccgctccgg tgtaaaggct
     2041 ccagccgccg cgcccttcgc aggattgatt ggcataaata tctgcggcaa tcgcataagg
     2101 ctccagccgg taagtgcggg acgactcttg atccagcgag cggtaagcgg ggttgatata
     2161 ttgcaagatc tgaaaagcat cgtctccgcg cccctctttc agaagcccca gcgccagcca
     2221 tatcgccgcg tgggtgtatt gccccccgtt ttcccggatt ccgggcggat aggaacagat
     2281 ataaccgggg ttacgtcccc cgccgtcaaa gggcggagtg aaaagacgga cgattttcag
     2341 cctgtcgtct ttcagcatct tgagcgcgga atccagtgcc gcctgtcgtc tttccttgtc
     2401 ggggaatcct gctatggccg aaaaactttg aggcaaaagg tcgatccggc attctccgct
     2461 ctctttccct ccgagcggga atccgtcgtc atagaacccc cggagatacc attttccgtc
     2521 ccaacaggtt tcgtcgacgg cctttctgag ttgagccgat tccgcaagat actgctcgcc
     2581 tctggtctcg tcaccctgtt ttttgcataa aggggcaaat ctctcaagca caatggagag
     2641 gaacatggcg agccatacgc tctcgccttt tccggctatg cccaccagat tcatgccgtc
     2701 attccagtcg cccccgccga aaagcggtat ctcgtgggcg ccaaggcgtt ttgcccgttc
     2761 aatggccctg acacagtgaa aatagaggtt ttctttttcc tccgagcgtt gagggaagaa
     2821 ataacggtcc tcctccttct gttccagttc cttgccgtca agataataaa cctcatgatc
     2881 aaaaatactg tcgtctcctg ttttttcaag atattcacaa agtgtgagag gaagccacag
     2941 catatcatcg gagcaacggg tgcgcacacc ccgggtcccg ccgtcccggg gaggcagctg
     3001 atgccaccag tgcatcacat cgccttcctt aaattggtgg gccgcactgc ggtagagatg
     3061 cgccttcgca aactgaggat caaacaaaag ggccgcgcag caatcctgaa gctgatcacg
     3121 gaagccccat gcgcccccgt tttgatagaa cccggttttt cccatgatcc tggacgacat
     3181 aaactgccaa cgcagccagg tatccatcag cgcgtccaga tacggatcgg gtgttgagac
     3241 ctttaatccg tcgccgctca ggggaccgtc cccagcggag cgggccggat ccgtgctttt
     3301 tagcattccc gccatcgcct gtgccgcacc cttttccgca gcccagccaa gcacaaaagt
     3361 tgccactgtt gttttccccg gcggaagctt tcttttcaca acagccgcag cgcaggagtc
     3421 aggcgatatg tcgtcgcccg cattttccca gtttccggag aggatacccg cccggttgta
     3481 aaggaatccg tccatccctt caagcgccgt aaggaaaccg gctccccgga ccggcgccca
     3541 ggcattccgc atctgaacaa cgccctgatc ctgctgaatt gatatatggc gtctggtcga
     3601 agcattcact cccataatcg gctcaacata atacgcgata cggagatgat attccttttt
     3661 cgcccggttc tcaatctcca gcctgaccag tttggccatc attttgccgg gtatctccac
     3721 cgtgacccga cagaacagct ggtcgatcac cgtttcataa accgcttttg acgggctgaa
     3781 tgtgactctc gcgttggcgc aaagatcaaa tattttattt cccactttca aaagaagcat
     3841 ttctccccgg ttgtcggcaa gaggatcgtt gttccatggc gtgagtttat tttcacggga
     3901 attgagcgcc caggtgaatc cgagtgcccg gtcggataac agcgtcccga aggttctgtt
     3961 ggccaaaacg tggcaccacg gtgcagaagg cttctgctca tgagagatga taaaatccgg
     4021 cccaagaaac tcaccgccgt aaactgcaag gttcttttcg gattctgccc gattggaaac
     4081 cgggtttcct gaattcagcg cagcgctctc ataatgatcg tccggcagtt ttttaccgac
     4141 cccggaggtc gccgtgtgac aggcggttgt aagcagcaaa taataaattt cttccgggtg
     4201 gatgctcagg ttcaccagat gaactccgcc ccggccgcca atcaggtatt cgcttccgca
     4261 ggatttgatc atatcattga gtacgctatg ctgctgcctt gaataatctc ccccctcccg
     4321 gtaggtgatg acaaggtccg cctgaattcc cttgagtcgg agagcccttt gggttttaat
     4381 atagggcagc agcctctcgg catcccgctg gccccggtat tcaaaaagaa tcagcggata
     4441 atctcccgaa ataccaaggc tccataaacc ctgctggctg agacggttgt ttgcaacagc
     4501 ctcccgttta tcgacataat ctttcacagg gaaaagaatt tgcggcagga tcagggctgc
     4561 cagacgcgtt tccatttcat tgctgtcttt ccccgccgcc gaacggatga aagcagaata
     4621 tccatgtctg cgggcttcaa tcagccgggc tgccgcttcg tcctctgaat ttgcggcggc
     4681 caacagcaga gtgcagttct tttttccgtg cggcggaagc tcggtggtga ttcgcagcgc
     4741 ggctgccgct tccggcaaag cgcccgttcc ggatttcatc tccgctttca gcgcttcagg
     4801 aagggatgcg atcccgtccg gccggctcag cagcctttcc cggtcggtat caaactcgaa
     4861 atccagctcc tgctcggcaa aaccgacggc cagaaaagcc ggcatctccc cgctgcgcgg
     4921 ccttcgcgca aaaataagaa ttttcgtatc ctcccgatag gacgcgtgaa gaaacaaccg
     4981 ggaaaacgcg ggatgcgccg cttcatcggc
//
LOCUS       DATALY010000265        13154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_573741,
            whole genome shotgun sequence.
ACCESSION   DATALY010000265 DATALY010000000
VERSION     DATALY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573741"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="metA"
                     /locus_tag="VHR42_07240"
     CDS             <1..915
                     /gene="metA"
                     /locus_tag="VHR42_07240"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEX3027005.1"
                     /translation="KVHDGLPAIKTLESENIFVITEDRAVHQDIRPLQIVVLNLMPTK
                     IETETQLLRLLGNTPLQVEIDLLQAVTHTSRNTSAEHLLEFYKSFDEIKDKKYDGMII
                     TGAPVELMEFEQVDYWKELCAIMEWTKHHVYSTLHICWGAQAGLYYHFGIPKYELPEK
                     MFGIFSHKLLEPTHPLLRGFDESFLAPHSRHTEIRMADIEKIPQLQTLSVSDEAGVYI
                     VANRNGRQFFVTGHSEYDRMTLAGEYFRDVERGLKTKLPRHYFPGDNPHATPLLTWRS
                     HADLFYSNWLNYFVYQETPFDLSNLPKP"
     gene            complement(1124..1624)
                     /locus_tag="VHR42_07245"
     CDS             complement(1124..1624)
                     /locus_tag="VHR42_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3027006.1"
                     /translation="MFRKMRRNKQALSVEESIAVLNNGTSGVLAVSGDDDYPYAVPLS
                     YVYYDSKIFFHCATSGHKLDAIAKNRKVSFCVIDKDNVMPQEYTTYFRSVIVFGKARI
                     LDNEDEKRSAFEVLAAKYSPDHERGRMQEIDKQFKQVCLVELTIDHMTGKEAIEFVRM
                     KNLQNN"
     gene            complement(1970..2359)
                     /locus_tag="VHR42_07250"
     CDS             complement(1970..2359)
                     /locus_tag="VHR42_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027007.1"
                     /translation="MMSINGINTQYQNYAASGTTGAKSSSGSSSFDAFQKEVVNWEKR
                     IKDNIDKEKENDSNGSIQMSEKQWRNLIKRVDYAIDALKGNVKEQEQEGKKQVEEKNL
                     TRKDTVAADSKTDNTPIQFLNRELVLR"
     gene            complement(2584..3603)
                     /gene="mreB"
                     /locus_tag="VHR42_07255"
     CDS             complement(2584..3603)
                     /gene="mreB"
                     /locus_tag="VHR42_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEX3027008.1"
                     /translation="MPGNDIGIDLGTATVLINIKGKGIVLSEPSVVAVDKGKDRIVAV
                     GAEAQKMLGRTPGNIVAVRPLRNGVISDYQLTEYMLKYFIKKVCGFFLFKPRIMICVP
                     SLITEVEERAVYDAAIQAGARKAYLIEEPIAAAIGAGIDITRPNGNMVIDIGGGTTDI
                     AVISLMDTAVTNSIKTAGDKFDEAIIRYIRKKYNVLIGERTAEELKKQIGCVFPREES
                     MEVSVKGRCLLTGLPKTLDITSEEMLEAFSEPAAEIAEAVHSVLERTPPELVGDIINN
                     GIVITGGGSLLYGMDQLIEKHTGISTYVADEPITCVARGTGAALDNLNALEDGLLYIS
                     RNKKG"
     gene            complement(3729..4925)
                     /locus_tag="VHR42_07260"
     CDS             complement(3729..4925)
                     /locus_tag="VHR42_07260"
                     /inference="COORDINATES: protein motif:HMM:NF022641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEX3027009.1"
                     /translation="MSGLIVKYRPFWSFLVQDTEKWLETLAGRGLHIKSVDFKKNKFV
                     FFEGEPVRCKYRFSYNKFETKRPQKENTLSKNGWEKLASYDKWVLYKTQNTNELNVIP
                     NRRGIYLRNNSLLLIYNVVSFIILLSAFIVSFTLLIFLSPNRTDSSFFNGIFVIVLIF
                     AVLIVANFLIFLRLSFTNSKILEEFGSSLNPQYAIYKHFIKHKTFEDWLEKLLVKEGD
                     IVKVFRPFWIFSPDKFENWLETMEARGMNFYKIGKPDFLIYFIRGNTRKMKYCLVGND
                     KANLDDTKMLLESDWNIVYSTTGQFGKFVLISKSYEGKKPGLFRRQADYRANAYRIIF
                     DYAALVLALLVILALYLVAAFVYPILFNRIWLALVAAVALLNVILIAKLAAYSVRVKK
                     RSKSLK"
     gene            complement(5132..5836)
                     /locus_tag="VHR42_07265"
     CDS             complement(5132..5836)
                     /locus_tag="VHR42_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX3027010.1"
                     /translation="MSSQWIRRVLAILFPERCAFCGSVISPGRKACAACEGQLPFVTG
                     EICLNCGRAKTFCVCGHKKREYSGCVVPFYYEGPAKKGIRRLKYSRRVSVAPALAGYA
                     APVIVRKFGGADFDFVTFVPMSTREKKSRGFNQSELFASALARMLDLPCLDVMTKPFE
                     NRPQHDCAETERWGNVFGAFEVREGSAVEGKTILLCDDVVTTGATLNECAKMLRLSGA
                     KKVFCSAAACVKKRGL"
     gene            complement(5829..8039)
                     /locus_tag="VHR42_07270"
     CDS             complement(5829..8039)
                     /locus_tag="VHR42_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX3027011.1"
                     /translation="MDQELVTLDGKIESVIFRNESNGWAVLEMDCNDELMTVVGNIFQ
                     VSEGEELKVMGEWVNHPSYGRQLRAVSFERKIPATASAILKYLSSGAVKGIGPSTAGK
                     IMEAFGDKTLEILENDPETLATVKGISLKKAQMISEEYRQQFGVRTLMLFLQQYNVTP
                     AESIRIWKRWGVDAVELIKKNPYVLCGNGLWIGFERADAIAREMGFEIGDPLRVEAGV
                     QYVLRHNLFSAGHTYIPRDKLSRTSAEMLGVTAAETDSGISELIDHKDLVGEDIAGRP
                     AVFLPDSYRCESYIAGRLELMRSLSPPSLGESSERIRQAEEEFSICYAGKQRLAIRTA
                     CESNLMILTGGPGTGKTTTLKAIIRVYEGMGLKVALAAPTGRAAKRMSEVSGKESKTI
                     HRLLEMEYADEEVPRFSRNEKNPLDCDALIVDELSMVDVMLLESMLRAMKLTCRLVMV
                     GDADQLPPVGAGNALRDMIESGRVPVVELDEIFRQAAQSLIVMNAHRIVRGEMPELKN
                     RKNDFFFLPCYTAAQVKSTVRELVETRLPAAYGYSPLWDLQVLVPGRKGEIGANSLNS
                     VLQQAINPPDKEKNERQTVSQILREGDKVMQIKNNYDITWLRENGEKGTGVFNGDVGV
                     LASIDRRTGMFRVRFDDRTAEYSPETVRELELAYAITVHKSQGSEFKAVVIPIFSGAP
                     QLFYRNLLYTAVTRAREMVVVVGRKEAVEKMVSNDRKTRRYTGLREFLTGGNHE"
     gene            complement(8231..8572)
                     /locus_tag="VHR42_07275"
     CDS             complement(8231..8572)
                     /locus_tag="VHR42_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027012.1"
                     /translation="MLEIIVGTAVAFFTIVGIVEVSRGVMERLLLPNGEKMIFLIASS
                     GHSESMEYRVRSLVYLAREMHLKSLPSIVIVDEGMDNETRKICELLASEFECVGICGN
                     GELSSLISANT"
     gene            complement(8705..9898)
                     /gene="metK"
                     /locus_tag="VHR42_07280"
     CDS             complement(8705..9898)
                     /gene="metK"
                     /locus_tag="VHR42_07280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX3027013.1"
                     /translation="MKNYFFTSESVTEGHPDKMCDQISDAVLDAILAQDPMGRVACET
                     TVTTGLVSVMGEISTSCYVDIPKIVRATIRDIGYDRAKFGFDCDTCAVITSIDEQSAD
                     IALGVDKALEAKEGSMNEAEIDAIGAGDQGMMFGYACNETPELMPLPISLAHKLARRL
                     AEVRKSGLLNYLRPDGKTQVTVEYENGRPVRVDTIVISTQHGPETDLATIRKDMIEQV
                     VTPVVPAELLDDRTKYFINPTGRFVVGGPAGDSGLTGRKIIVDTYGGYAAHGGGAFSG
                     KDPTKVDRSASYAARHVAKNIVAASLADKCQVEIAYAIGVAHPVSIMVDTFGTNKIDE
                     DKIAKIVNELFDLRPAAIIRDLDLRKPIYRELAAYGHMGREDLGVTWEKTDMVEKLRK
                     AAFDR"
     regulatory      complement(9985..10088)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(10198..10773)
                     /locus_tag="VHR42_07285"
     CDS             complement(10198..10773)
                     /locus_tag="VHR42_07285"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX3027014.1"
                     /translation="MQSQKTRLIVFTGLFTALSVMLYFFEFPIVPGLSYLKVDFSDLP
                     AVLAAVMFGPAAGIAAELIKNLFHLMVRGFGDTMGFGDLMNFLVGCAFLVPFSMTLRA
                     LLKRAKGYAFSAIAAGLAGMAGMTLMGVVGNYLIAPPFFRFMLHIQLSNTALWAAIGG
                     ATVLNILKSVILVLLLIPTASLCLRFVNHKH"
     regulatory      complement(10870..10998)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            11419..12867
                     /locus_tag="VHR42_07290"
     CDS             11419..12867
                     /locus_tag="VHR42_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3027015.1"
                     /translation="MKSQKNAPLLDALRKYANQEITHFDVPGHKKRNNPEIIDAYGEK
                     TLKMDANSTPELDLPANPTGVIAQAEQLLADAYWADQAFLLVNGSTAGVQYMILTACG
                     PNDKIIMPRNVHKSAINALILCGARPVFIEPEIDCEFGINNGVRPDSVKKAIQENPDA
                     RAVFLINPTYFGATSDLREIVKLAHRYKMTVLVDESHGAHFPFHPDFPVYAMDAGADM
                     ATISLHKTGGSLTQSSALLLNERVYSRSRIRSTINLLQTTSASYLLMSSIDLARRKLS
                     IQGEKIFGELLPVIRKAKQEISQIPGLAVLSKSNRNGAGIFDYDETKFVIRVNDLGLT
                     GFEAYELLRKDYGIQPELAETYVVLAIASIGDDAQTIRKLVDALRDLSTRFYGKKERF
                     SVPMKGFLEIPRTVLSPRDAHYSSRRSVSLDEAVGEICGESIMIYPPGIPLAIPGERL
                     TEEIIEHYKFYQTQKCVLMNDDEHADQITILG"
     gene            12909..>13154
                     /locus_tag="VHR42_07295"
     CDS             12909..>13154
                     /locus_tag="VHR42_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX3027016.1"
                     /translation="MSITDLSESFLSARLIGCDADYSKSGAVVFGAPFDGTVTFRPGS
                     RFAPSRIRSESFGLETYSPYLDRDLEDYAVHDAGDLDL"
BASE COUNT         3440 a         3480 c         2789 g         3445 t
ORIGIN      
        1 aaagtacacg acggacttcc tgctataaag acactcgaaa gtgagaacat atttgtaatc
       61 accgaagacc gcgccgtcca tcaggatata cggccgcttc agattgttgt tttaaacctg
      121 atgccgacta agattgaaac agagacccaa ttgctccggc ttcttggaaa cacgcccctg
      181 caagttgaga tcgacctcct tcaggccgtc acccacacct cgcggaacac ttcggcggag
      241 catctgcttg agttttataa aagcttcgat gaaatcaaag acaagaaata tgacggaatg
      301 attattacag gcgcgcctgt cgaactgatg gagtttgaac aggtggatta ctggaaagaa
      361 ctttgcgcca ttatggaatg gaccaaacac catgtctatt ccacactgca tatctgttgg
      421 ggcgcgcagg cgggattgta ttaccatttc ggcattccaa aatatgagct gcccgagaaa
      481 atgttcggta tattctcaca taagcttctt gaaccgacgc atccgctcct gagaggattc
      541 gacgaatcat ttcttgctcc tcattcacgg catacggaaa tcaggatggc cgatatcgaa
      601 aagattccgc agttgcaaac cctttccgtt tctgatgaag ccggcgtcta tattgtagca
      661 aatcggaacg gacgccagtt ttttgtcacc ggccattccg aatatgaccg gatgacgctc
      721 gcgggtgagt atttccggga tgtcgaacgg gggttaaaga ccaaattacc caggcattac
      781 tttccggggg ataatcccca tgccactccg cttctgacct ggcgcagcca cgcagatctc
      841 ttttattcca actggctgaa ctattttgtc tatcaggaaa cgccatttga cctttctaat
      901 ctcccgaaac cttgaattca ccgaaaaacc acgacgggac caacaatgcc tcgctgaatg
      961 cgcagagtgc atcagggagc aactaccaaa cataaaaaaa aggatagact tccaactgat
     1021 aagatatata cgacaaaaac ggatgccgca accgggcatc cgttttttat cagggttcaa
     1081 tttcgggcca acttgcgaag tttttcgcgg gcggcctatt aacctaattg ttttgcagat
     1141 tcttcattct aacaaactca atggcttctt taccggtcat gtggtcaatc gtgagctcta
     1201 caaggcatac ctgtttgaac tgtttgtcaa tttcctgcat acgtccccgt tcgtggtcag
     1261 gcgaatattt tgcggccaaa acttcaaaag cactgcgttt ttcatcttca ttgtctaaaa
     1321 tccgtgcttt tccaaagaca ataacgctgc ggaagtaagt ggtatattct tgcggcatca
     1381 cattatcctt gtcgatcacg caaaaggaaa cttttcgatt ttttgcaata gcgtccagtt
     1441 tgtggcctga tgttgcacaa tgaaaaaaga ttttagagtc gtaatataca tagctgagtg
     1501 ggaccgcata gggataatca tcatccccgg atacagccag aacgccggaa gttccattat
     1561 tcaaaacagc aatactctct tccacggata acgcctgttt gttgcgacgc attttccgaa
     1621 acataaaggc acctctttca tcggtaaaat aataaaacgt ctataattca ttcctttaaa
     1681 ggcctaatgg aatgaactaa agacgtttac ccggcggcaa tgatctgtta ttaactctgt
     1741 tataatagca tatttacacc aaaaagcaat agcattaaag gattcggcaa aaatcgatac
     1801 gcagtaaaat tccgccgccc gttcggaact ttccggaggg gctttcctta tgccattcgt
     1861 attcttttgg aatttcagaa gatccaatat gaattctatc ggttcagccg taagtatgtt
     1921 gaaaattctg gtgatcacca tcggcgtgat tacagcaaac ggttttacgt taccttaata
     1981 ctaattcccg atttaaaaat tgaataggag tattatccgt cttggaatct gcagcaacag
     2041 tatctttccg ggtcaagttt ttctcttcca cctgtttttt tccttcttgt tcctgctcct
     2101 tgacattacc ttttaaggca tcgatcgcgt agtccactct tttgattaaa ttgcgccatt
     2161 gcttttctga catctgaata ctgccattgc tgtcgttttc cttctcttta tcaatattat
     2221 ccttgatccg cttttcccag ttcacgactt ctttttgaaa cgcatcaaaa gaactggaac
     2281 cactgctgct ttttgcgccg gtggtaccgg aagctgcata attttgatat tgtgtattta
     2341 ttccgttaat gctcatcatg tattccctcc agataaataa attctgcccg aagaaaaagt
     2401 caaaaaacgt ggtgccataa attgtatatc tttattgtag cattcttatc caattttttc
     2461 aacaacacga atttcgcctt tgataggagc aagcgaatta cacacgtttg caagatcagt
     2521 attacaaatt gcgcaggcat atgcctgcgc aatttgttga tctaaaagac aatgaaccgc
     2581 tgattagccc tttttgtttc gggaaatata cagcagcccg tcctctaggg cattcaggtt
     2641 atcaagcgcc gccccagtgc ccctggccac gcatgtgatc ggttcgtcag caacataagt
     2701 gctgattccc gtatgttttt caatcagttg atccatgcca taaaggaggc tgccaccgcc
     2761 cgtaatcaca attccattgt tgataatatc ccccacaagc tctggaggcg tacgttccaa
     2821 cactgaatga accgcttcgg caatttccgc cgcaggctct gaaaaagcct ccagcatctc
     2881 ttccgacgtg atatcaagag ttttaggcag accagtcagc agacatctgc ccttgacgct
     2941 gacctccatg ctttcttctc ttggaaaaac gcatccgatc tgcttcttca gctcctcagc
     3001 cgtccgttcg ccgatcagaa cattatattt ctttcggata taacggataa tggcttcgtc
     3061 gaatttatcc ccggccgttt taatggaatt ggtcacagcg gtatccatca gtgagattac
     3121 cgcaatatcg gtcgtgccgc cgccgatgtc gatgaccata tttccattcg ggcgggtaat
     3181 gtcaattccg gccccgattg cggcggcaat cggctcctca attaaataag ccttacgcgc
     3241 gcctgcctga atcgctgcgt cataaaccgc gcgttcttcc acttcggtga taagacttgg
     3301 cacgcaaatc atgatccttg gtttgaacaa aaagaaaccg cagacttttt ttataaagta
     3361 tttgagcata tattcggtca gctgataatc agaaatcact ccgttacgca gcggtctaac
     3421 cgcaacgata ttacccggag tacgcccgag catcttctgg gcctcagcgc ccactgcaac
     3481 aatacggtcc ttacccttat cgaccgcaac aaccgatggt tcgctgagga caattccctt
     3541 gcccttaata ttaataagta cggttgctgt accgaggtcg attcctatgt catttcctgg
     3601 caattacatc acctctgtct ttgattgaaa acaaatattg aaaacaaatt cgggataaaa
     3661 tgcatcccct tattttatta taatcaaaaa tcatccgaaa taaaacataa ataccgaatt
     3721 tttaccttct attttaaact tttcgaccgt tttttcactc tgacagaata agcagcgagt
     3781 tttgcaatca gaattacatt cagcagcgct accgccgcca ccaaagccaa ccaaatccgg
     3841 ttgaataata tgggatatac aaatgcagcc acaagataaa gagccaaaat caccaataaa
     3901 gccaacacca gtgcggcata atcaaaaatg atccggtacg catttgcgcg gtagtccgcc
     3961 tgccttctga aaagccccgg cttttttcct tcataacttt tcgaaataag aacaaatttg
     4021 ccgaactgcc cggtcgtgga ataaacaata ttccagtcgc tctccagcag catttttgta
     4081 tcgtccagat tcgctttgtc gtttccaacc aggcagtatt tcatctttct tgtgttgccc
     4141 ctgataaagt aaatcaaaaa atcgggcttg ccgatcttat aaaaattcat acctctggct
     4201 tccattgttt ccagccagtt ttcaaatttg tcgggagaaa atatccagaa gggccggaat
     4261 actttaacga tatccccttc tttgacaagg agtttttcca gccaatcctc aaatgtttta
     4321 tgcttaataa aatgtttgta gatcgcgtac tgaggattca gtgaggaacc gaattcctcg
     4381 agaattttgc tgtttgtaaa ggaaagtctc aaaaaaatca aaaaatttgc gacaatcagc
     4441 accgcaaaga tcaatacgat cacaaaaatg ccgttaaaaa agctgctgtc cgtccggttc
     4501 ggcgagagga aaatcaataa ggtgaaactg acgataaatg cgctgagcag aatgataaaa
     4561 ctgactacgt tataaatcag cagcagggaa ttatttctta gataaattcc acgccggttg
     4621 ggtatcacat tcagttcatt cgtattttgt gttttataaa gtacccattt gtcataggat
     4681 gcaagctttt cccagccgtt tttcgaaagg gtattctctt tttggggcct ttttgtctcg
     4741 aatttattat agctgaaacg atatttacat cttaccggtt ccccttcaaa aaaaacaaat
     4801 ttattttttt tgaaatcgac cgatttgata tgtaatcccc gtcctgcaag cgtttcaagc
     4861 catttttccg tatcctgaac aagaaaactc caaaaaggcc tgtatttgac aatcaaaccc
     4921 gacatttata atgcccccgt caagagataa tatctcgcgt accggtcgcc gtctgatatc
     4981 cggcagatca ggctaaaaaa cttttcggaa accggaggtt tctgatttca tacagattaa
     5041 ttatatcatc aatattatat catgtcattg tcaacctata ctgtgttttg gttaatttgt
     5101 ccatttaatt ggtataattc gttatgtttg gtcagagtcc gcgttttttt acacaggcgg
     5161 cagcggaaca gaagactttt tttgctcccg aaagccgaag cattttcgca cactcattca
     5221 gagttgcccc ggttgtgacg acgtcgtcac agagcaggat ggtttttcct tcaaccgccg
     5281 atccttcccg gacttcaaac gcgccgaaca catttcccca tctttctgtt tccgcacagt
     5341 catgctgagg cctgttttcg aaaggtttcg tcatcacgtc cagacagggc aaatccagca
     5401 tccgggccag tgccgaggcg aataattccg actggttaaa accgcggctc ttcttttccc
     5461 gagtgctcat ggggacaaag gtaacaaaat caaagtctgc cccgccgaat ttccggacaa
     5521 tcaccggagc cgcatacccg gccaacgccg gcgcgacgga aacccggcgc gaatacttca
     5581 gcctgcggat tccctttttg gccgggcctt cgtaataaaa gggcacaaca cagccggaat
     5641 actcgcgttt cttatgaccg cagacgcaaa aggtttttgc ccgcccgcag ttcagacaga
     5701 tttctcctgt gacaaaagga agctgccctt cacaggcggc gcaggctttc cggcccggcg
     5761 aaatcacgct tccgcaaaat gcgcagcgtt cagggaacag gatggctaac acacgcctga
     5821 tccattggct actcatgatt tccacccgtc aagaactccc gaagccccgt atagcgtctg
     5881 gtctttctgt cgtttgacac cattttttcg accgcttctt tccggccgac aaccactacc
     5941 atctctctcg ctctcgtaac cgccgtatag aggaggtttc ggtaaaacag ctggggagcc
     6001 ccggaaaaaa tcgggatcac caccgcttta aattcacttc cctggctttt gtgaacggta
     6061 atggcatagg caagctcgag ctcccgtacg gtttcgggcg aatattcagc ggttctgtcg
     6121 tcaaatcgca cccgaaacat cccagtacgc cggtcgatgg aggccaatac accaacatcc
     6181 ccgttaaaaa caccggttcc tttttcgccg ttttctctca gccatgtaat atcatagttg
     6241 tttttgatct gcatcacctt atcgccttcc cgcaaaatct gggagacagt ctggcgctca
     6301 tttttttcct tatccggcgg attaatggcc tgctgcagga cggaattcag tgaatttgcc
     6361 ccgatctccc cttttctgcc gggtaccagc acttggaggt cccacagcgg cgaatagccg
     6421 tacgcagccg gaagtctcgt ctctaccaat tctctgaccg tgcttttcac ctgcgccgcc
     6481 gtataacacg gaagaaagaa aaaatcgttt ttccgatttt taagttcagg catttccccc
     6541 cggacgatcc ggtgggcatt cataacaatc agactctggg cagcctgccg gaagatctcg
     6601 tcaagctcaa ccacgggcac acggcctgat tcaatcatat ccctcagcgc gttgcccgcc
     6661 ccaaccggag gcagctgatc ggcgtccccg accattacaa gtctgcaggt cagtttcatt
     6721 gccctcagca ttgattcaag cagcatgaca tccaccatgg aaagttcatc gacaattaac
     6781 gcatcacagt cgaggggatt tttttcatta cgggaaaaac gcggaacctc ttcgtcagca
     6841 tattccattt caagcagacg gtgaatggtt ttagactctt tcccggaaac ctcagacatc
     6901 ctcttggcgg cccgtccggt aggcgcggcc agcgcgacct tcagccccat accttcataa
     6961 acacggataa tggcttttaa ggtggtcgtt tttcctgttc ccggcccgcc tgtcaagatc
     7021 atcagattac tttcacaggc agtgcgtatg gcaagcctct gtttgcccgc atagcaaatg
     7081 gaaaattcct cttcagcctg ccggattctc tccgagcttt cccccagcga aggcggcgac
     7141 aaacttctca tcagttccag ccggccggca atatagctct cgcagcgata ggaatccggg
     7201 agaaatacgg cgggacggcc ggcaatgtct tcaccgacga gatctttatg atcaatgagt
     7261 tccgagatgc cggaatcggt ctccgccgct gtcactccca gcatttcggc tgaggtgcgg
     7321 gacagcttgt ccctcggaat ataagtatga cctgcgctga aaagattatg gcgcagcaca
     7381 tattgaacgc ctgcctcaac acgcagcgga tcaccgattt caaatcccat ttcgcgcgcg
     7441 atggcgtcgg ctctttcgaa accgatccaa aggccgttcc cgcatagaac atagggattt
     7501 tttttgatca gctccaccgc gtcgacgccc caccttttcc agatccgaat ggattctgcc
     7561 ggcgtaacat tatattgctg caaaaaaagc attagcgttc gcacgccgaa ttgctgtcgg
     7621 tattcttctg aaatcatctg cgcttttttt aatgaaattc ctttgactgt cgcaagcgtt
     7681 tccggatcat tttctaatat ctccaacgtc ttgtcgccaa aggcttccat aattttaccc
     7741 gctgtcgacg gcccgatccc ctttaccgcg ccagaagaaa gatattttaa aatcgcggaa
     7801 gcggtcgcag gtatttttcg ttcgaaagaa accgccctga gctgcctgcc ataagacggg
     7861 tggttgaccc actcgcccat gactttcagt tcctcgcctt ctgaaacctg aaaaatattt
     7921 cccacaaccg tcatcagctc gtcgttacag tccatttcca aaacggccca gccattactt
     7981 tcatttcgaa atatgaccga ttcaatttta ccgtcgagtg ttaccaattc ctgatccatt
     8041 tatttcaaac ccgctctgtt tttacaattg gttctcaaaa taccgttgtt cactcactca
     8101 aattatacta tataccggag aatttcacaa cctgcaggag accggcccta aaaacaaaag
     8161 cgcggaaacc caaaacggtt cccgcgcccg gagcccggcc ggcagactgg tttttccact
     8221 gcggcaaagc ctaagtattc gcactgatca gcgatgataa ttcaccattg ccgcaaatgc
     8281 ccacacattc aaactcgctg gcaagcaatt cacagatttt gcgggtttcg ttatccattc
     8341 cctcatctac aatcacgatg gacggcaagg atttcaggtg catctctctt gccagataca
     8401 caagggaacg tacccggtat tccatgctct cgctatggcc gctggaagca atcagaaaaa
     8461 tcattttttc tccgtttggc agtaacaagc gctccataac tcccctgctg acctcaacga
     8521 ttccgacaat cgtaaaaaat gcgacggcag tcccgactat aatctccaac atcgccacca
     8581 cctcatatta tcatatgagg acaaaacccg atttgttact tttttttctg ttttttcact
     8641 cttaaatcca atttgttacc atttaaagac cgagccggcg aaattcccgc cggcccaact
     8701 tgactcagcg gtcaaatgcc gcttttctta acttttcaac catatcggtc ttttcccagg
     8761 tcacgccaag atcttccctg cccatgtgac cataggccgc gagttctctg taaatcggtt
     8821 tgcgcaggtc aagatcacgg ataatcgccg cagggcggag atcaaacagt tcgttgacga
     8881 tctttgctat tttgtcttcg tcaatcttat tggttccaaa ggtatcaacc ataatggaaa
     8941 ccggatgggc cacaccgatt gcgtaggcaa tctctacctg gcacttatca gccagtgacg
     9001 cggcaacaat gtttttcgca acatgccggg ccgcatagga tgcggaacgg tcaactttcg
     9061 taggatcttt tccggaaaac gctcctccgc catgagcggc atatccaccg taagtatcga
     9121 cgatgatctt ccggccggta aggccggagt ctcccgcagg cccgccgaca acaaaacgtc
     9181 cggtcgggtt aataaaatat tttgttctgt catccagaag ttccgccgga acaacggggg
     9241 tcacaacctg ctcgatcata tctttccgga tcgtcgcaag gtcggtttcc gggccatgct
     9301 gcgtggagat gacgatcgtg tccaccctca caggcctgcc gttttcatat tcaacggtga
     9361 cctgggtttt tccgtccggc cggagataat tcaggagtcc gctcttccgg acttcggcca
     9421 gccggcgcgc cagcttgtgg gcgagtgaga tcggcagggg catcagttcc ggggtctcgt
     9481 tacaggcgta gccaaacatc ataccctgat cacctgcgcc aattgcatcg atttcggcct
     9541 cgttcatgct gccttccttt gcttcaagcg ccttgtccac gcccagcgcg atatctgcgg
     9601 attgctcgtc aatcgacgtg attaccgcgc aggtatcgca gtcaaagcca aactttgccc
     9661 tgtcatatcc gatgtcacgg atagtagctc ttacaatttt aggaatatca acataacagc
     9721 tggtcgaaat ctcgcccatc accgaaacaa gaccggtagt gaccgtcgtc tcgcaggcca
     9781 cgcggcccat cggatcctga gccaggatcg catccagaac cgcgtcggaa atctgatcgc
     9841 acattttatc cggatgccct tccgttactg attcagatgt aaaaaaataa tttttcataa
     9901 agctcctcct tagccgaaaa tataaaatgg ttggtaaaaa atcaaaaagc tccccggtaa
     9961 atacaaccgg agagcaaaaa ttcatcctca tcttcacggt tgacctaccg caggaattag
    10021 caccttgcga aaaatccgca ggttgccggg cttcatcggg cctttccctc tgcctctctt
    10081 gataaggtcg atattcattt tactgtatta tatccaacat tttgcttaat gtcaacatga
    10141 tctgacattg ttcataaaat tttaatttaa tattggtggt atttttcctt gagcttttta
    10201 atgtttgtga ttgacaaacc gcagacaaag cgacgcggtc ggaattaaca gcagaaccag
    10261 aataaccgac ttcagaatat tcaggacggt cgctccgccg attgctgccc aaagagcagt
    10321 attgctcaac tgaatatgaa gcataaagcg gaaaaacgga ggggctatca aataatttcc
    10381 aacgaccccc atcagcgtca tcccagccat accggcaaga ccggccgcaa tagcggaaaa
    10441 cgcatagcct ttggctcgtt ttaaaagcgc gcggagcgtc atcgaaaacg gcaccaaaaa
    10501 ggcacagcca accaaaaaat tcatcaggtc gccgaacccc attgtatcac cgaatcccct
    10561 gaccatcaaa tgaaaaagat ttttgatcag ctccgccgca atcccggccg ccgggccaaa
    10621 catcacagcg gcaagaacgg cgggcagatc gctgaaatcc actttcagat aactcagccc
    10681 cggtacaatg ggaaactcaa aaaagtaaag catcaccgac agcgccgtaa acaaacccgt
    10741 aaatacaata agcctggttt tctgtgactg cattttctta cctccataat ccggggtaca
    10801 ggccgccgca atccgaaatt tccaaacaaa aaccgggtga ctttcatcac ccggggcttt
    10861 cctgattgct gccgtcttct ttcatccgga ctttaccgtc ggtcacggaa tcacaccgta
    10921 tctgctttcg ctctcggact tgtcggcttt cgccgcatta ccgccggtgg ggaatcacac
    10981 cccgccccaa agacatttat tttatttgtt attaaaatat catactcttc ctgttttgtc
    11041 aacgtttttt gtgggaaaga taattcccaa agcaaccctg tttttttcaa aaaagcgcga
    11101 acttttaccg attagacgct ttctgacttt ttttgtttta atctattgaa aattgataag
    11161 tttttctcta taatgattat gcttttcagg tgggcgcagc gtccgtctga cagcattgaa
    11221 caccgccgca gattttagcg gcaccgtaaa aaacgggaat taacaaacca ggactgaggt
    11281 gataccgata accacaacgt tgctgaacgg tacaaattct atccttttcc gctgtaattt
    11341 tacacctgaa atttgatacc ttctccacga acacaatcga cagggtaatt acataagaag
    11401 aaggagaaaa agaataaatt gaaaagccaa aaaaatgcgc cgcttcttga tgcgctccgg
    11461 aaatacgcca atcaagagat tacccacttt gacgtgccgg ggcacaaaaa aagaaataat
    11521 ccggaaatca tcgacgcata cggagaaaaa acgctgaaaa tggacgccaa ctcaaccccg
    11581 gaacttgatc tgcccgcaaa tcccaccggc gtcatcgccc aggccgaaca gcttctggcc
    11641 gacgcctatt gggcggatca ggcctttctt ctggtcaacg gttccacggc aggggttcag
    11701 tatatgattc tcacggcatg cgggccaaac gacaaaatca ttatgccgag aaacgtccat
    11761 aaatccgcaa tcaacgcgct tattctgtgc ggcgcgcggc ctgtgtttat cgaacctgaa
    11821 attgactgtg agtttggaat caacaacggg gtccgtccgg actcggtcaa aaaagccatt
    11881 caggagaatc ccgacgccag agcggttttc ctgattaatc ccacgtattt cggggccaca
    11941 tcggatcttc gtgaaattgt caaactcgcc caccgatata aaatgacggt gctggtagat
    12001 gaatcgcacg gcgcacattt cccctttcat ccggatttcc ccgtatatgc gatggatgcc
    12061 ggcgctgata tggccaccat cagtctgcat aaaaccggcg ggtcgctgac ccaaagctcg
    12121 gcccttctgt taaacgagcg ggtttacagc cgttcccgca ttcgctcaac aatcaatctt
    12181 ctgcaaacga ccagcgcttc ctatctgtta atgtcgagta ttgatcttgc cagaaggaaa
    12241 ctctccattc agggcgaaaa gatttttggc gaactgctgc cggttattcg aaaagcaaag
    12301 caggagatct cgcagattcc cgggcttgcc gtcctgtcga aaagcaatcg aaacggagcg
    12361 ggaatttttg attacgacga gacgaagttt gtcatccgcg tcaacgatct cgggctgacc
    12421 gggtttgaag cctacgagct cctccgcaaa gattatggga ttcagccgga gcttgccgaa
    12481 acctatgtgg ttcttgcgat cgcctccatc ggagacgacg cgcagacaat ccgcaaactg
    12541 gtcgacgcac ttcgggatct gagcacgcgc ttttacggaa aaaaggagcg cttctccgtc
    12601 ccgatgaaag gttttctcga aattccaagg accgttcttt caccgagaga cgcccattac
    12661 agctcccggc gctccgtctc gcttgacgag gccgtcggag aaatctgcgg agaatccatt
    12721 atgatctatc cgcccggaat tccgctggct attcccgggg agcggctgac cgaggaaatt
    12781 atcgaacact acaagttcta tcagacccag aaatgtgttc tgatgaacga tgacgaacac
    12841 gccgaccaga tcaccatact gggctgagaa aaggctatcc gtcaaagacg caaaggagat
    12901 atcagagcat gagtatcaca gacctgagcg aatcgtttct ttccgcccgc ctgatcggct
    12961 gcgacgcgga ttattcgaaa agcggagcgg tggttttcgg cgctcctttt gacgggacgg
    13021 tcacttttcg gccagggtca agatttgccc cctcgagaat ccggtcggaa tccttcggcc
    13081 ttgaaaccta tagtccctat ctcgaccgcg atctcgaaga ttacgccgtt cacgacgcgg
    13141 gcgatctgga tctt
//
LOCUS       DATALY010000266         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_662967,
            whole genome shotgun sequence.
ACCESSION   DATALY010000266 DATALY010000000
VERSION     DATALY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662967"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..455
                     /locus_tag="VHR42_07300"
     CDS             36..455
                     /locus_tag="VHR42_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3027017.1"
                     /translation="MVMTLEADYAVRIVEMLARDSQKIDAHNISLQTQVPLRFALKIL
                     RKLVAGGIVVSYKGAHGGYALAREPGKITLRQVIESVEGPYMLNRCQQEAYCCTHTSY
                     CRFQKIYDEISLLVRERLDACTFESLQKAGNDAKPET"
     gene            753..>1805
                     /locus_tag="VHR42_07305"
     CDS             753..>1805
                     /locus_tag="VHR42_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX3027018.1"
                     /translation="MVNVKPVKVGKNTRMSFARIDEVLDMPNLIEVQKESYRWFLDEG
                     LKEVFKEVSGITDYTGNLVLDFVDYKLDTDKPNYSVEECKERDATYSAALRVTARLLN
                     KESGEIKESEVFMGDFPLMTDSGTFVINGAERVIVSQLVRSPGVYYKMEFDKTGKELY
                     SSTVIPNRGAWLEYENDANDVFYVRIDKNRKIPVTVFIRSLGLGTDQEIVDYFGNDDR
                     INATIEKDPCKNTEEALFEIYRKLRPSEPPTIESAQSHLNSLFFDARRYDLSRVGRYK
                     YNKKLNLAGRLTGQVLSQPIANPLTGEVLAEAGTVVSRELAVQIDDAGVSLAHVQVGD
                     REVKIISNGMVDINKFV"
BASE COUNT          491 a          403 c          464 g          447 t
ORIGIN      
        1 aattaaaaga ttttctggat taaggggaca gtttcatggt aatgacactg gaagcggatt
       61 acgcggtgcg cattgtcgaa atgctcgcgc gtgacagcca aaagatcgat gcccataaca
      121 tctccctgca gacacaggtt cctttgcggt ttgctctgaa aattcttcgg aagctggtcg
      181 cggggggaat cgtggtttcc tacaagggtg cccacggcgg ttacgcgttg gcgcgtgaac
      241 cggggaaaat cacgctgcgg caggtgattg aatcggtgga ggggccgtat atgctgaacc
      301 gctgccagca ggaagcttat tgctgcacgc acacaagcta ctgcaggttc cagaaaatat
      361 acgacgaaat ttctctgctg gtacgcgaac ggcttgacgc ctgtacattc gaatccctgc
      421 aaaaggcggg aaacgatgca aagcccgaaa catagagaat aaaagaaaag agccggtaaa
      481 cccggctctt tttccgtcat tcataaaaat ttttcctgta aattgggtga aattataaaa
      541 ataacttgac actaatcaca ttttgttata aaataatata ctgcatcatc atggaaactt
      601 gtatgttcct atgattctgt aattaatcat atcacaagtt cttcgaaaag gcaagagcta
      661 ttaaaattgg gtgggagacg aaagcttttt tgtgcttatg tgcttaattt atgccgcaga
      721 ctgtgaaaaa aatgaatgga gtgattgagc caatggtgaa tgtcaaaccc gttaaggtcg
      781 gcaaaaatac ccggatgagt tttgctcgta ttgacgaagt tcttgacatg cccaatctga
      841 tcgaggtgca gaaggaatcg taccgctggt tcctcgatga ggggttaaag gaggttttca
      901 aggaagtttc cggaataacg gattatactg gtaatctggt tctggacttt gtcgattaca
      961 agcttgacac ggataaaccg aattacagtg tggaggaatg caaggaacgc gatgccacct
     1021 attcggcggc cctgagggta acggcccgac tgctcaataa ggaaagcggc gaaattaaag
     1081 aatccgaagt tttcatggga gactttccgt tgatgacgga cagtggaacc tttgtcatca
     1141 acggcgcaga gcgcgttatt gtttcccagc tggtccgttc tcccggcgtt tattataaga
     1201 tggaattcga caagaccggc aaggagctgt acagctctac cgtaatcccg aatcgcggcg
     1261 cctggctgga atatgagaac gatgcaaacg acgtctttta cgtccgcatt gataaaaacc
     1321 ggaaaattcc cgtcacggtt ttcatccgtt ctttgggtct cggcaccgat caggaaatcg
     1381 tcgattattt cggcaatgac gatcggatca acgctaccat tgaaaaggac ccgtgcaaga
     1441 atacggaaga agcactgttt gaaatatacc gcaagctgcg cccgagcgag cctcccacca
     1501 ttgagagcgc tcagtctcat ttgaacagcc tctttttcga tgcccgccgt tatgatcttt
     1561 cccgtgtcgg gcgctataaa tacaataaga aactgaatct tgccgggcgg ctcaccggcc
     1621 aggtgctttc acagccgatt gcaaacccgc tgaccggcga agttcttgct gaggctggaa
     1681 ccgtcgtgtc gcgtgaattg gcggttcaga tcgacgatgc cggagtttcg cttgcccacg
     1741 ttcaggtcgg agaccgcgaa gtcaaaatta tctccaacgg aatggtggac atcaataaat
     1801 tcgtc
//
LOCUS       DATALY010000267         8052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_402019,
            whole genome shotgun sequence.
ACCESSION   DATALY010000267 DATALY010000000
VERSION     DATALY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402019"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2214)
                     /locus_tag="VHR42_07310"
     CDS             complement(<1..2214)
                     /locus_tag="VHR42_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX3027019.1"
                     /translation="MKRFAKTGLAGLLSAAIILTMAVPVSATSPKALLGDPLDSMLTL
                     NNASVTTTSPDATTLRKEVKKVSGNSKQFDVTLTLTAPTQGKTQSTDIVLVMDTSGSM
                     GYSNGSGGTRLSDAKSAAKKFVDTVLKSSYPKNQVALVSFGNSVTTFTDSSTHSNFVN
                     YNNAGDIQNAIGQLHANQGTFMQGGIKAAESLLSGSSADNKYIILLSDGYPNYSLGAT
                     QGVQSPQTWNGWSGWRQAKNSSSYTPENYILTAFGTTLKGTGNPYSGYQLNGSGANTK
                     PYSVSGVTGKVNNNGVATISEAYAAQHSGITMYSIGCNISGNAQADYVLTNCASTNDH
                     YYSVASTNLNDVYQTIAGQIVWPSVVVKDAIGADFSYVGLTDAINPNPSSGNAAEDPS
                     SKVVTWRVSSLQGTQKLVYRVQINSDVTDGSHSIDVSNTSVPTGLTVDSGTAYDFPSQ
                     PYVDVGTGSVTIIPYLSDSSGAPIDTSGNPVATKSDAALSYGSYTPSNPQVLTSLKLQ
                     NNYNVPGIAGYISPDSNVYTLNAAQQFKLTLDQPTQTVYVPYTMDKTIHIKYMELKSD
                     GTTAALDVSSTGNPTSIHGAIGSTYDISSQLKPVNGYGLVTAQSDPLSGKFKCDSSDI
                     TLLYSKQSYDYFVQYYKDGAKFGGQVKLGSAEFQSSVKADQLTNLYGNKPVGYKVGDV
                     QNLPLTISNDLSKNVINVYYTKDSYDYYVQYYKDGAKFGGQVKLGSAEFQSSVKAD"
     gene            complement(2379..2792)
                     /locus_tag="VHR42_07315"
     CDS             complement(2379..2792)
                     /locus_tag="VHR42_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027020.1"
                     /translation="MAESRKHELVGWIAFRGGEDRFAFSGAADLLLKMDRMYDELSLP
                     KAAFSGRRFGKIKNIQKKEKAGECKLDISEPDREKSAVFIIKVRFRQNATWQGQIEWI
                     NENRIQYFRSELELLRLMDYAVSSVFGDDDGVAWE"
     gene            3098..3499
                     /locus_tag="VHR42_07320"
     CDS             3098..3499
                     /locus_tag="VHR42_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3027021.1"
                     /translation="MKKQDNLVESQKEKVESISDSEWLVAKILWERSPQTSAEIIDEL
                     KQSTVWSPKTIHTLIRRLAQKKIIAAVEGVTPYRYYPLLSEAECEREKTRSFVERIYN
                     GSFNSMVSRFVEEKMLSKAEIEELKDILNGK"
     gene            3515..5089
                     /locus_tag="VHR42_07325"
     CDS             3515..5089
                     /locus_tag="VHR42_07325"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3027022.1"
                     /translation="MLIKVFKVVLLLSIECGMLSIAFLLLRTLLWKRIHPQLQYLLWG
                     ILILRMLLPIAPQIPINVFTLFPKNITFTQSANQLAGTPSSKASSVSSNIHAKSTPEN
                     STPLKKNSSLPDSSVTFPNNLTLLWNAAAVVWLSGCIVFLLYTLVVNLRLSRKLRRIG
                     RSRNARLNSELDNCRQILKIRRPVSIICDPAFRSPAAFGLIYPCIILSPVLADELTDE
                     DIRYIFLHELSHIRRHDNEINLLTVVIRSIHWFNPVLWLALAKMKCDCEISCDAAVLK
                     ILSKSERRAYGRTIVSVIESLASPVIHPAAIGFADGFSKRRISIIVSHKNASAICSAA
                     AVTLAVLTGFCSLPAKEPAKIPVANSSAGTSQVSSVSGGALTAKSKSRSASVSSGSKS
                     DSDKVLSLKPKNYKSLDDFAAKNGYQIEMDSGRTGTLKLPKSFLTVSQNEKVGSYFKE
                     CNETSKKYGLDFSSYLGKTATLIDYDTEKSDGTCAYLFGLYADHKLIGIWISPHDPAS
                     NDLRSTMHFGGLYTNG"
     gene            5119..5355
                     /locus_tag="VHR42_07330"
     CDS             5119..5355
                     /locus_tag="VHR42_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027023.1"
                     /translation="MSDMKFEGKTDLKKNTCFWFCIPVAAFLLVSAIVILTYGFSLNR
                     YLLYILIVFDVFLVFALFRLLFQYYKLKKSLNHP"
     gene            5398..6666
                     /locus_tag="VHR42_07335"
     CDS             5398..6666
                     /locus_tag="VHR42_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX3027024.1"
                     /translation="MRSVSFCRRAIACALILLLTISICGCSLIDSGRDSSGAAISNDS
                     SSAGVSSASTLESSSSPAQTSGPANASAAGRDTSRPLTSGEFVRVWMEQTNNGISKNV
                     SVAQAVSFAKQRGIIPADASGDSVLTIQKAAYLLTGAKTIDLINSHYDWQIRDLDQVR
                     PTLKKYLLAAYSKGLLTTAGGYIRPNQELYAKDVLSILARLKNPGERILPPNCAAPYF
                     EYRGLVEVIRLDPTVIIDQRYATKNNFTGVAHYQRSICLLEAVTARRLCQANAYFKQK
                     GRRIKIWDGYRPVSVQWSLYYATPANLKQYAPAPSKYSQHSKGIAADITLTDKNGIEL
                     PMPTGFDNFTAKAHSDYSGLSSRIIANRSDLKTGMTKQGFAQSKLEWWHFYSPDKTGL
                     SISKVELNDFSQRENDFYQNYLSHNRKSPS"
     gene            complement(6768..7964)
                     /locus_tag="VHR42_07340"
     CDS             complement(6768..7964)
                     /locus_tag="VHR42_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX3027025.1"
                     /translation="MSEWNGSEPISVTMFGEFTVKTGGRVLTDRFGRAKRIRNLLEYL
                     IANRNGDSSQEKLIEVLWGDTECRDPANALKNLVYRLRNLMGTPDGMDYIIFRHNTYA
                     WNNELPCVVDIEEFKKAWEQGRRAEGESEKLKAFYRAAELYQGEFLPGSSMQDWVVRM
                     STGFAGMFLEGVNEICRIYAAREEYEKIVEICQRAMKTNPYAESLHEKMIWAYLRSGD
                     RQRAVFQYEYSSGFFYDKLGVKLSDRIGSAVYKTARSLKGLETDMVVIKKDLRESDEV
                     NGAYFCDYEQFRSAYHVMARICGRTGASVFLALLTVAPSGKKSPDNQKVSEAMQTLKG
                     AIVSSLRKSDLVARFSSTQFILMMPSLTYENGKMVLERILRKYDQTGNIRGITVFTRL
                     NPLETV"
BASE COUNT         2045 a         2066 c         1745 g         2196 t
ORIGIN      
        1 gtcggcctta acggaagact ggaattcagc cgaacccagt ttgacctgac cgccgaactt
       61 cgctccgtct ttgtagtact gcacatagta atcataactg tccttggtgt aatagacatt
      121 gatgacgttc ttggacagat cattgctgat cgtcagcgga agattttgaa cgtcgccaac
      181 cttgtagcca accggtttgt ttccgtaaag gttggtgagc tggtcggcct taacggaaga
      241 ctggaattca gccgaaccca gtttgacctg accgccgaac ttcgctccgt ctttgtagta
      301 ctgcacaaaa tagtcatagc tttgctttga atacagaagc gtgatgtctg atgaatcaca
      361 cttgaacttc ccactcagcg ggtcagactg agctgtgacg agaccatagc cgttcactgg
      421 ctttaactgg ctgctgatgt cataggtcga cccaattgcg ccatgaatgg atgtcggatt
      481 acctgtcgac gaaacatcca gcgctgcagt ggtaccgtcg ctcttaagtt ccatatactt
      541 aatatggatg gtcttatcca tggtataggg aacatagacc gtttgggtcg gctgatcaag
      601 cgtaagcttg aactgctgcg ctgcgttcag cgtataaaca tttgaatcgg gcgagatata
      661 gccagctata cccggaacat tataattgtt ttgcagtttc aggctggtta acacctgggg
      721 attggacggc gtatagctgc cgtagctcaa agctgcatct gatttcgtcg caaccggatt
      781 cccgcttgta tcgatcggtg cgccgctgct gtcggaaaga taaggaataa tcgtgaccga
      841 accggttccc acatccacat atggctggga tgggaaatca taagcggtgc cggaatccac
      901 tgttaatccc gtcggtacgc ttgtgtttga aacgtcaatc gagtggcttc catcggtaac
      961 atcggaattg atctgcacac ggtaaaccaa tttttgcgtt ccctgcagag aggaaaccct
     1021 ccatgtgacc actttgctgg aaggatcttc ggccgcattt ccggacgacg gattgggatt
     1081 gatcgcatcc gtcagcccga cataactgaa gtccgcacca attgcgtctt ttacaaccac
     1141 acttggccag acaatctgcc cggcgatggt ttgatacaca tcgttaagat ttgtgctggc
     1201 tacgctgtaa taatgatcgt tggtgctcgc acagttcgtc aaaacataat cggcctgcgc
     1261 attgcccgaa atattgcatc cgatagaata cattgtgatt cccgaatgct gggccgcata
     1321 tgcttccgaa atggttgcga ctccgttatt attgactttt cctgtcactc cgctcacact
     1381 gtagggtttg gtgtttgcgc cactcccgtt gagctgataa ccgctataag gatttccggt
     1441 accttttagc gttgtgccaa acgccgtgag aatatagttt tcaggcgtgt agctgctgga
     1501 attttttgct tgcctccagc cgctccaacc gttccatgtt tgcggagact gtactccctg
     1561 cgtggcgcca aggctgtaat tgggatagcc gtcgctcagg agaatgatat atttgttgtc
     1621 tgcgctgctg ccggaaagca gactttccgc agctttgatc cctccctgca taaaggttcc
     1681 ctgatttgca tgcagttgac caattgcatt ttggatgtcg ccggcattat tgtagttaac
     1741 aaagttcgaa tgggtcgatg agtcggtgaa agtagttacg ctgtttccaa aagagacaag
     1801 cgcaacctgg tttttcgggt aactgctttt caacacggta tctacaaact ttttagcagc
     1861 gcttttcgcg tcgctcagcc gggtcccgcc ggaaccattg gaataaccca tacttccgga
     1921 ggtatccatc accaggacaa tatccgtact ttgggttttt ccctgcgtcg gggccgtcag
     1981 tgtcagggta acatcaaatt gcttgctgtt tccggaaacc tttttgactt ccttcctcag
     2041 tgtggtagca tccggagaag tggtcgtaac cgacgcgttg ttcagcgtca gcatcgaatc
     2101 cagtggatct cccagtaatg cctttggtga agttgctgat accggtaccg ccattgtcag
     2161 aatgattgca gcggaaagca gccctgcaag acctgttttt gcaaatcttt tcatacttct
     2221 cctcctttca tttgactttt tgaaaattga ctgctgattc tcccaccttc ttttccactt
     2281 tctccaaacg attatttcaa ggatctctat ataaaaatga ataataggaa gctcaataaa
     2341 aggaacggga taaagcggtt atcataaatg gccgcagatc actcccaggc aaccccgtca
     2401 tcatcaccga aaacggaact gaccgcgtaa tccatcagcc ggagcaactc aagctcactt
     2461 ctgaaatact ggattctgtt ttcattgatc cactcaatct ggccctgcca ggtggcgttt
     2521 tgccggaatc gaaccttgat gataaacact gctgattttt ctctatccgg ctcgctgata
     2581 tccaatttgc attcacccgc tttttctttc ttctggatgt ttttaatttt tccaaaacga
     2641 cggcccgaaa aagccgcctt cggtaacgac aattcgtcat acatccgatc cattttcagc
     2701 agaagatcgg cggctccgct gaaggcaaat ctatcctctc ccccccggaa agctatccat
     2761 cccaccagct catgttttct gctctctgcc acaacaaggg cagctgagct caaaatcttt
     2821 ccgaatgaat tgtatgtcga ccaaaacatc ttttattcac ccgcaaccaa attggaaata
     2881 ttaaccattt attattatga agattcctag tcattttcca gtcacatatt gaaattggat
     2941 aatatttcca tataaaattg taaaaaaaga accgcctttt cacggcggtt cagtaagaat
     3001 atcattcaat cttgaaataa gcgtcgggaa acaattgaca attcgatttt tatcacttac
     3061 aatagacgta ttacaattgt aatacgaggt gaaccgcatg aaaaagcagg acaatttagt
     3121 ggaaagccaa aaagaaaaag tggaaagtat ctccgactcg gaatggcttg ttgccaaaat
     3181 tctttgggaa cgctcccccc agacatccgc cgagattatt gacgagctca aacaatccac
     3241 tgtctggagc cctaaaacca ttcacacgct gatccgacgg cttgcgcaaa aaaagattat
     3301 tgccgcggtg gagggcgtca cgccataccg gtattatcca ttgctttccg aggccgaatg
     3361 tgaacgtgaa aaaacgcgat cgtttgttga aagaatttac aatggttcct ttaattcaat
     3421 ggtttcccgc tttgtagaag aaaaaatgct ctcaaaagcg gaaattgaag aattgaaaga
     3481 tattctcaac gggaaatagg aggctcgctt gaatatgctg ataaaggtat ttaaggttgt
     3541 tctgttgctt tctatcgagt gcggcatgct atcaattgca tttttgttac tgcgtacgct
     3601 gctatggaaa agaatccatc cgcagctgca atatctgctg tggggaattc ttattctgcg
     3661 gatgttactg cccatagctc cgcaaatccc aatcaatgtt tttacccttt tccctaagaa
     3721 tatcaccttc actcagagcg caaatcaatt agccggaaca ccatcttcaa aagcttcatc
     3781 cgtttcgtca aatattcacg caaaaagcac gccggaaaac tcaacgccac tcaaaaagaa
     3841 ttcaagtttg cctgattctt ctgtaacgtt tccaaataac ctgactcttt tatggaacgc
     3901 cgctgcggtt gtgtggcttt ccggctgcat agttttctta ctttatacat tggttgttaa
     3961 tctgagattg agccgaaagc tgcgcagaat aggccggtcc cgcaacgcac gtctgaattc
     4021 ggaactggac aattgccgcc aaatcttaaa aatccggcgc cccgtatcca ttatttgtga
     4081 cccggctttc cgttctcctg cggctttcgg tctcatctac ccctgcatca tcctttcgcc
     4141 ggttcttgca gacgagttga ccgatgaaga tatccgctat atttttcttc atgaactctc
     4201 acatatccgc cgccatgata atgaaatcaa tctgttaacg gtggtgatcc ggtccattca
     4261 ctggttcaac ccggttctgt ggctggcgct tgccaaaatg aagtgcgact gtgaaatttc
     4321 ctgtgatgcc gctgttctta agattctttc aaaatcggaa cgtcgggcct atggcagaac
     4381 aattgtcagc gttatcgagt ctcttgcatc accggtaatc catccggccg cgattggctt
     4441 tgccgacggg ttcagcaaaa gaagaatctc aatcattgta agccacaaaa atgcgtcggc
     4501 aatctgctcg gccgccgccg taacgctcgc ggtcctgact ggattttgca gcctccccgc
     4561 aaaagagccg gccaagatac ctgtggcaaa ttcatccgca ggtacttcgc aggtttcgtc
     4621 cgtctcaggc ggcgctctca cggccaaaag caaaagtcgt tccgcctccg tatcgtcggg
     4681 ctccaagtcg gattctgata aggttctatc cttaaagcct aaaaactaca aatcactgga
     4741 cgattttgca gcgaaaaacg gctatcagat cgaaatggac agcggacgga cgggaacttt
     4801 aaagctgccg aagagctttt taactgtcag tcaaaatgaa aaagtcggca gctatttcaa
     4861 ggaatgcaac gaaacatcca aaaaatatgg acttgatttt tcttcctatc ttgggaagac
     4921 tgccacgctt atagattatg ataccgaaaa aagcgatgga acctgcgcct atctgttcgg
     4981 cctgtatgct gatcacaagc tgatcggcat ttggatttct ccgcatgatc cggcatccaa
     5041 cgatcttcgc agtacgatgc actttggagg cttatacaca aatggttaaa gaaataactg
     5101 aaacagggag cgaataaaat gtctgatatg aaatttgaag gcaaaaccga tctcaaaaaa
     5161 aacacctgct tctggttctg tattcccgtc gcagcgtttc ttcttgtttc ggccatcgtc
     5221 attctaactt acggtttctc gcttaaccgc tatttactgt atattttgat tgttttcgat
     5281 gtctttttgg tttttgcact tttcaggctg ctctttcaat actacaaatt gaaaaaaagt
     5341 cttaatcatc cgtaaaacct ttgtctttaa ttcttaatgg aaaacgaggc aattttaatg
     5401 aggagtgtat ctttttgccg ccgtgcaata gcgtgtgctt tgattttatt gctcacgatt
     5461 tctatttgcg gctgcagcct tatcgattcg ggacgggatt cttccggtgc agccatatct
     5521 aacgattctt ccagcgccgg ggtttcctcc gcttctactc ttgaatcttc ctccagtccg
     5581 gcccaaacct ccgggccggc gaatgcaagc gcagccggcc gcgacacttc ccgtccgctc
     5641 accagcgggg aatttgtccg ggtatggatg gaacagacaa acaatgggat cagcaaaaac
     5701 gtttccgtgg cccaggcggt atctttcgcc aaacaacgcg ggataattcc cgcggatgct
     5761 tccggcgact ccgttctcac aatacaaaaa gccgcgtatc ttttaaccgg ggccaaaacg
     5821 attgatttga tcaactccca ctatgactgg cagatccggg atctggatca agtaagacca
     5881 acattgaaaa aatatctgct tgccgcctac tccaaagggc tgctgacgac tgcgggcgga
     5941 tacatccgcc cgaatcagga actttatgca aaagacgttt tgtctatcct tgcacgactg
     6001 aaaaatccgg gcgaacggat tctccctccc aattgcgccg caccctattt tgaataccgt
     6061 gggctggttg aagtgatccg tcttgatccc accgttatca ttgaccaaag gtacgccacc
     6121 aaaaataatt ttaccggtgt cgcgcactat cagcggtcga tctgcctgct ggaagccgtt
     6181 acggccaggc gtctctgtca ggcaaacgcc tattttaaac aaaaaggccg ccgcatcaaa
     6241 atctgggatg gctaccggcc cgtttccgta caatggtcgc tttattatgc cactccagca
     6301 aatttaaagc agtacgcgcc cgctccttca aagtactccc aacattccaa aggcatcgcc
     6361 gcggatatca cgcttaccga taaaaatgga attgaattgc cgatgccgac cggtttcgat
     6421 aacttcaccg ctaaagcaca ttccgattac agcggtcttt ccagtcggat cattgccaac
     6481 cgaagcgatc tcaaaacagg aatgacaaag caggggtttg cgcagagcaa gctggagtgg
     6541 tggcattttt attcaccgga taaaaccgga ctttccatct caaaagttga gctaaatgac
     6601 ttttcacaga gggaaaacga tttttatcag aattatctca gccataacag aaaaagccct
     6661 tcatgatcct acgcatttga aagcagccca attttccccc ccaagaacgc aaagcaggcc
     6721 gccggaatcc ggcggcctgc tttgcgttct tgatctattc gataacctca aaccgtttcc
     6781 agagggttca ggcgggtaaa aacagtgatt ccgcgaatgt ttccggtttg atcatacttc
     6841 cgaagaatcc tttcgagaac catctttcca ttttcatagg taagtgacgg catcataagg
     6901 ataaactgag tgctgctgaa ccgggcaacc agatcgcttt tcctcagact tgaaacaata
     6961 gcacccttta aagtctgcat tgcctcggaa accttttggt tatccgggct ttttttcccg
     7021 gagggagcca ccgtcagcag tgcgagaaac acggaagccc cggttcttcc gcagattcgg
     7081 gccatcacat gataagcgct tcgaaactgt tcgtaatcgc agaaataagc cccgttgact
     7141 tcatccgatt cccgcagatc tttttttatc acgaccatgt cggtctccaa gcctttcaga
     7201 ctacgcgccg ttttataaac tgcgctgccg attcggtcgg aaagtttcac tcccagtttg
     7261 tcataaaaaa atccactcga gtattcatat tgaaacacgg cgcgctgtct gtcaccggat
     7321 cgaagatacg cccaaatcat cttctcatgc agcgattcgg cataggggtt cgttttcatc
     7381 gcccgctggc aaatttccac gattttctca tattcttctc tcgcagcata aatccggcag
     7441 atttcgttga caccctccag aaacatcccc gcaaaacccg tgctcatgcg gacaacccag
     7501 tcctgcatgg aggatccggg caaaaactcc ccctgataca attccgccgc ccggtaaaaa
     7561 gctttcagct tctcgctttc accctctgcc ctcctgccct gttcccaagc cttcttaaac
     7621 tcctcaatat cgacgacaca tggcagttca ttgttccatg cgtaagtatt atgacggaag
     7681 ataatatagt ccatgccgtc cggggtcccc ataagatttc gaagccgata aacaagattt
     7741 ttcagcgcat tggcagggtc tctgcattcc gtgtcacccc aaagaacctc gattaacttt
     7801 tcctgggaac tgtcaccgtt tcgattggcg atcaggtatt ccagcaggtt ccgtattcgt
     7861 ttcgcccgtc caaaccggtc ggtcaatact ctgccgcccg ttttaaccgt gaactctccg
     7921 aacattgtga cactgatcgg ctccgacccg ttccattccg acatgttttg tcactcccga
     7981 aacatttaga aatatttacc agttatcagg ataacatatc ttttttccaa tattcaacat
     8041 ttattccccg gc
//
LOCUS       DATALY010000268        11757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_402118,
            whole genome shotgun sequence.
ACCESSION   DATALY010000268 DATALY010000000
VERSION     DATALY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402118"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /gene="prfA"
                     /locus_tag="VHR42_07345"
     CDS             complement(<1..949)
                     /gene="prfA"
                     /locus_tag="VHR42_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX3027026.1"
                     /translation="MFDKLEYIEKRFNEIAVKLSDPEIVSDQEQYKKLMKEYKSLTGI
                     VEKYREYKKARQDAEEAKELLESHLDRDMKEMAEEELNVSRASMEKIAEQLKVLLLPS
                     DPNDEKNVIVEIRGGAGGEEAALFAANLYRMYSMFAETKGWKTEVLNLNATELGGIKE
                     ISFMIEGDGAYSRLKFESGVHRVQRVPETEASGRIHTSTVTVAVLPEAEEVELQINPD
                     ELQIDTYRSGGAGGQHVNKTESAIRITHLPTGLVVECQDERSQFKNKDKAMKILRSRL
                     YEKMQSEQAAKIATERRSQVGTGDRSERLRTYNYPQGRVT"
     gene            complement(966..1154)
                     /locus_tag="VHR42_07350"
     CDS             complement(966..1154)
                     /locus_tag="VHR42_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX3027027.1"
                     /translation="MDVKAGDTIVLKKPHPCGCNRFLVLRTGVDFKVKCEKCGHEIMT
                     PRIKIEKKIKSVIKSGEY"
     gene            1335..1694
                     /locus_tag="VHR42_07355"
     CDS             1335..1694
                     /locus_tag="VHR42_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027028.1"
                     /translation="MSVIVSAVFDSEDLADLAAARVKREVSGIESISIVENRYANEHQ
                     PSQSYIITGGQYTNYYRSYAPVPVFPLDDDSSINYYEPAERQDALLRMEVDNGGSKII
                     SSMLRNMGGRQIRQIKK"
     gene            1768..2694
                     /locus_tag="VHR42_07360"
     CDS             1768..2694
                     /locus_tag="VHR42_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX3027029.1"
                     /translation="MTVIKPEGWLLETLENREALRTPGGIMKAREEGRTLEARALICD
                     SGHNLIVDLDCMRGIIPREEGALGLDTGATRDIAVISRVNKAVSFKVKDIKHQPDGKN
                     VAILSRRAAQEEAMRLSISRFMPGDVIDARVTHLEPFGCFVDIGCGIPSLIPIDLISV
                     SRISHPRDRFRVGQDIKVVIKSIEGDQRICLTHKELLGTWEENAARFTPGETVAGIVR
                     SVENYGVFVELTPNLAGLAEPKEGVRPGQCASVYIKNLIPEKMKVKLIIVDSFDAEYK
                     PEEPFYYINSGHLSRWTYSPADCDKVIETVFE"
     gene            complement(2736..3254)
                     /locus_tag="VHR42_07365"
     CDS             complement(2736..3254)
                     /locus_tag="VHR42_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEX3027030.1"
                     /translation="MKKTKIIVFTGLFIALNVVLDRFLSIQTDIVRISLGFLPIALTG
                     YLFGPVAGGLAGALSDIVGMIVFPKGAYFPGFTLTGLIGGAAYGLILNKKPVSILRVT
                     AVVLAVAAVCDLGLNTFWLYIITGKAAFAIFIPRLVKTAIMIPIQVFLIYSVSKLIGA
                     AKLMSTAQINKN"
     regulatory      complement(3328..3427)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            3627..4910
                     /locus_tag="VHR42_07370"
     CDS             3627..4910
                     /locus_tag="VHR42_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX3027031.1"
                     /translation="MTLYVIKPGDSIYTIANKFGVSPQKIITANGLENPGQLVVGESI
                     IIPQGQKVHTVAAGESVYSIARKYGISQSSIIQANPFINAVGRIYPGQVLTIPVPGDE
                     KLGEIEVNGYIFPGSDEAIVRPWLKSLTYLSIFSYTVNADGSLPAIDDDKWIQIARSN
                     RVAPIMVITNIRPGGGFSTEIAHAILSNQTVQNNLIENVVRILKQKNYYGLNIDFEYI
                     QPTDRQNYNDFLRKITDRLHALGYIVLTAVAPKLNAEQKGLLYEAHDYPAHGSIVDRV
                     ILMTYEWGYLAGPPQAVAPLSQVKRVLDYAVSVIPPKKILMGIPNYAYDWVLPYVRGT
                     LASTFSNGEAVARAFRNKAEIKYDLNAQSPYYNYYDAQKRQHIDWFEDARSIYQKLLT
                     VDQYGLGGVSYWTIGRSFQQNLFLVDAMFNIKKVI"
     gene            5035..6093
                     /locus_tag="VHR42_07375"
     CDS             5035..6093
                     /locus_tag="VHR42_07375"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX3027032.1"
                     /translation="MEKYVVTIGAGHGGHDCGVVGPSGVMEKDAALNLSLRTAELMNS
                     TGCLRAIPIRSGDVYIDTARRAEIIADSGSRCHIELHFNALDSRADYTEVWHSFAQAA
                     DRAHAEYLSRRIAQVLDIRDGGPKARIDYGALLGRTILHEDYLGLFELLEKRRIPHVF
                     LAECGCIDHITTEQKINDLYYMDRIAQAITQTVCEIFSLSGLKLRLSKQQQPQIISPK
                     AETVFLKNGSFQVFSGPGPYYSTVRIIRGLIYTRCYQSLNGWIKISPSSEEWISATAI
                     KNFLDRKNVILPDCEATAERVGKAGFFYVKSAPDVRSDVLGAVTGGERWTTISENGWR
                     KICFDGRIGYVGAYPWQE"
     gene            complement(6204..7256)
                     /locus_tag="VHR42_07380"
     CDS             complement(6204..7256)
                     /locus_tag="VHR42_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3027033.1"
                     /translation="MKFKLFVCVVLAVACVLPMASCKSGGKTSSVTLNVYNWGEYIDE
                     SVNKEFTKQTGINVNYKTYATNEELYAKLKSGGVDYDVIIPSDYMVSRLSSENMLYKL
                     NFSNIPNFKDIGKNYLNLKYDAKNEYSVPYMWGTVGIVYNKTMVSDPVDSWDILWNSK
                     YKGKILMFDNSRDAIGIALKKLGYSYNTTDQAQIKKAAAELINQKPLVQAYVMDQIFD
                     KMEGGEAALAPYYAGDAVTMMQDNADLAFAVPKEGTNLFVDAMCVPKTSGHKTEAEKY
                     INFLCSPDISVKNCEETGYSTPSTAALKKLPAEIRSNTIAYPAEAILKKAEVYTNLPQ
                     NILDLYDSQWLDVISK"
     gene            complement(7246..8058)
                     /locus_tag="VHR42_07385"
     CDS             complement(7246..8058)
                     /locus_tag="VHR42_07385"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027034.1"
                     /translation="MKTSRRFLNRFYLYLVLAFFYLPIFVLIFYSFNASKSRTVWGGF
                     TLKWYGKLFSDQMILNSVYVTLAVSVLAALIATALGTAAAIGIMAMKKRMRTVFVNVT
                     YLPILSPEIIMGVSLMLLFVWACGSLNFGTLLLAHVSFDTPYVIFSVMPKLRQLDPHL
                     YEAAQDLGAHPATAFRKVVLPQIRPGIITGMLLAFTLSVDDFVISYFTSGDNSQTLSI
                     AIYAMTRKRVSPEINALSTLLFLFVLLFLIIINVRQAGGENSQQRKEIRDEI"
     gene            complement(8055..8870)
                     /locus_tag="VHR42_07390"
     CDS             complement(8055..8870)
                     /locus_tag="VHR42_07390"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027035.1"
                     /translation="MRKAHLSALPYLVWSILFTVVPLILVIGFAVTDRSNHLTFANFE
                     QMGIYTTVFVRSVVLALISTLLSLIIGYPVAYILSREPAKRQNTMLMLIMLPMWMNFL
                     LRTYAWLTLLENNGLINRFLGIFGIGPLHMINTQGAVVLGMVYNYMPYMILPIYSVLV
                     KLDHRLIEAAQDLGATPRRCFLKVVFPLSMPGVMSGVIMVFMPSVSTFIISKMLGGGS
                     NLLIGDLIEMEYLGNAYNPHLGAAISLVMMIIILISMWAMGFQKGDDKEVMVI"
     gene            complement(8867..9926)
                     /gene="potA"
                     /locus_tag="VHR42_07395"
                     /pseudo
     CDS             complement(8867..9926)
                     /gene="potA"
                     /locus_tag="VHR42_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
     gene            complement(10251..>11757)
                     /locus_tag="VHR42_07400"
     CDS             complement(10251..>11757)
                     /locus_tag="VHR42_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX3027036.1"
                     /translation="LSCVLLEMVLMAFFRYSYRGFLAASARFRRWSRHPAHSKVLVVA
                     GPLDGKALIEKIRADNREKTVVAVVDPDESHESGYYISGVRVYGCGLKSVESSIRLMQ
                     ADEVVASRNGLLESKLLDIVRICSENRCRLLVDSGIQKYDGIRNRVEKIQTGDLLGRV
                     QTEFTLPARRVIKNKTVLVTGGAGSIGSEICRQSLEYGCAKLVIFDIHENGLFEIDAD
                     LKKTYPRQKYQIVVGSVRDAGSLDRLFAEYRPDVIFHAAAHKHVPLMESNPAEAVKNN
                     VFGTFNVARFAIRYRAEKFILISTDKAVKPTNVMGATKRVAEMIVEHLNVGSKFTRLA
                     AVRFGNVLGSNGSVVPLFKRQIMHGGPVTVTHPDIRRFFMTIPEAVQLVMQAGELTRG
                     GEIFVLDMGEPVKIADLAAEMIRMSGFEPQKDIKIEFTGLRPGEKMYEELSLDDEDVQ
                     KTVYDRIFVCKSSRLARNLEEDLKTLKMVADNGEDAGCKAALKEIVAEYNS"
BASE COUNT         3042 a         3042 c         2724 g         2949 t
ORIGIN      
        1 cggtcacgcg cccctgagga tagttatagg ttcggagacg ttcgctgcgg tctccggttc
       61 ccacctggga gcgtctctcc gttgcgattt tcgcagcctg ctcactctgc attttttcat
      121 aaagtctgga ccggaggatc ttcatggctt tgtccttatt tttaaactgg cttctttcat
      181 cctgacactc cacgaccagc ccggtgggca gatgggtgat acggatggcg gattcggttt
      241 tgttgacatg ctggccgccc gcgccgccgc tgcgataggt gtcgatctgg agttcgtcgg
      301 ggttaatctg aagctcaact tcttccgctt cgggcagcac cgccaccgta accgtggagg
      361 tgtgaatccg cccggacgcc tctgtttccg gaacccgctg cacccggtga acgccgcttt
      421 cgaatttcag gcggctgtaa gcaccgtcgc cctcgatcat aaagctgatc tctttgatgc
      481 ctccgagctc ggttgcgttt aagttgagca cttcggtctt ccagcctttg gtttcggcaa
      541 acatggaata catcctatat agattggcag caaaaagcgc cgcttcctcg cctcccgctc
      601 ctccgcggat ttcgacgata acgtttttct catcgttggg gtcggagggc agcagcaaaa
      661 ccttgagctg ctccgcgatc ttttccatac tggctctgga gacgttcagt tcttcctcag
      721 ccatttcttt catatcccgg tcgagatgcg actccagtaa ttcttttgcc tcttcggcat
      781 cctgtctggc cttcttatac tcccgatatt tttcaacaat accggtcagc gacttatatt
      841 ccttcatcag ctttttatac tgctcctgat cggagacaat ctccgggtcg gacagtttta
      901 ccgcgatctc attaaaccgc ttttcaatat attcaagttt atcaaacatt aatacaccgc
      961 caattttagt attccccgga ttttatgaca cttttgatct ttttttcaat tttaatccgc
     1021 ggcgtcatga tctcgtggcc gcatttttcg catttcactt taaaatcgac tcctgtccgc
     1081 aacaccagaa aacggttgca gccgcaggga tggggctttt tcaagacgat tgtatcgccc
     1141 gctttcacat ccatcagtcc acctccgcgt agggatctga gagcctgaaa atccctgatt
     1201 tttgaacttt tacgattctt attattataa cacaattctg gattcttagg caaatttttt
     1261 cttgtatcaa aaccactaaa gaacaaataa tagaaacagt tccaataaaa tctgaatggt
     1321 tggaggatca ggctatgagc gtaattgtca gcgcggtttt tgattcggag gatcttgccg
     1381 atcttgccgc cgccagagta aaacgtgaag tttcgggaat agagagcatc tccattgtgg
     1441 aaaaccggta tgcaaatgag catcagccca gtcagagcta tatcataacc ggcgggcagt
     1501 atacaaacta ttaccggtca tacgcccccg ttccggtctt cccgctcgac gacgattcaa
     1561 gcatcaacta ttacgagccg gcggaaaggc aggatgccct cctgcgcatg gaagtcgata
     1621 acggcggctc taaaatcata tcatccatgt tacgaaatat gggtggcaga cagattcggc
     1681 aaatcaaaaa ataaatattg aatcagttaa ttattcacct gcctctgcat ataaattata
     1741 aggacgcaaa agagggaggt aaatcaaatg actgtgataa agccggaagg atggctttta
     1801 gagactttgg aaaatcggga ggctctcagg acacccgggg gaatcatgaa agcgcgcgaa
     1861 gagggccgca cgcttgaagc gcgggccctg atttgtgaca gcgggcataa tttgatcgtg
     1921 gatttggatt gcatgagggg aattattccc cgcgaagaag gcgcgcttgg ccttgacacc
     1981 ggggctaccc gggatattgc agtcatatcc cgagtgaaca aagcggtcag cttcaaggtc
     2041 aaagacatca agcatcagcc cgacgggaaa aacgtggcga ttttatcacg ccgcgcggcc
     2101 caggaagagg ccatgcggct ttctatatcc cgctttatgc caggtgacgt gattgatgca
     2161 agagtgactc atcttgagcc gtttggctgt tttgtggata tcggctgcgg cattccttcc
     2221 ctgattccca tcgatctgat ttcagtctca agaatttcac acccgcggga ccggttccgg
     2281 gttggccagg atatcaaggt tgtcatcaaa tccatcgaag gcgatcagcg tatctgcctg
     2341 acgcacaagg agcttcttgg aacctgggaa gaaaacgccg cgcgttttac acccggggag
     2401 accgtcgcgg gaattgtccg ctccgttgaa aactacggag tatttgtgga gctgacccca
     2461 aatctcgccg ggctcgccga gccgaaggaa ggggtacgcc ccggtcagtg cgcaagcgtt
     2521 tatatcaaaa acctgatccc ggaaaaaatg aaggtcaaac tgattatcgt ggattccttt
     2581 gacgctgaat acaaacccga agaaccattc tactacataa attccggtca tctcagccga
     2641 tggacctact cgcctgccga ctgtgacaaa gtcatagaaa ctgttttcga ataagacagg
     2701 cgccttaccg cttcatgcgg aaggcgcctt tacttttagt ttttatttat ctgggccgta
     2761 ctcatcagtt tcgcagcacc gatcaatttt gaaaccgaat aaatcaaaaa cacctgaatc
     2821 ggaatcataa tcgccgtctt aaccaggcgg ggtataaaaa tcgcaaacgc cgcttttccg
     2881 gtgatgatat aaagccaaaa tgtgttcaaa ccaaggtcac agactgcggc aacggcaagc
     2941 acaacagccg ttacccgaag gatggagacc ggttttttat tcaggatcag cccgtaggcc
     3001 gcgcctccga tcagccccgt cagggtgaac cccgggaagt aggccccttt gggaaacaca
     3061 atcataccga caatatccga cagagcgccg gccaacccgc ccgcgaccgg gccaaacaga
     3121 tatccggtaa gggcaatcgg aagaaatccc agactgattc ggacaatatc ggtctggatg
     3181 gacaggaacc tgtcaagcac tacattcagc gcgataaaca ggccggtaaa tacgatgatt
     3241 ttggtttttt tcaataaaaa aacactcctt ctcttaaata tggcaatcac gccacatgaa
     3301 gagaagtgtg ctgctgctct tttctgtggc aacggaatgc gacgactgca ccgcaaatca
     3361 cgcgattttt cgtccagcgg caacttcccg tccgtctggc acttgacgcg cagccatcta
     3421 ctttgccttt caacgatatt ataaatagcc ggtattattt tgtcaaatcc aatctcttac
     3481 agtgtcagtt tttgaagatg gctcatatat taaaatgaat gcgtccggtc gcagagccgg
     3541 ataatggaac tccgcttgaa ttcgcaatga gccggctgga acggccgccc ccgatattga
     3601 ttttcaagaa aagaagggtg atattcttga cactgtatgt gatcaaaccg ggcgacagca
     3661 tttacactat cgccaataaa tttggggttt cgcctcaaaa aatcatcacg gccaacgggc
     3721 tggagaatcc ggggcaactc gtggtcgggg aatccattat catcccccag ggtcagaaag
     3781 tccacacggt cgcggcgggt gaatcggttt attccattgc ccggaaatac ggtatttccc
     3841 aaagcagtat tattcaggca aacccgttta tcaatgccgt gggccggata tatcccggcc
     3901 aggttctcac gataccggtt ccgggagatg aaaagctggg ggaaattgaa gtgaacggct
     3961 atatctttcc cggttccgac gaggcaatcg tccgcccctg gctcaaaagt cttacatact
     4021 tgagtatctt cagctatacg gtcaatgcgg acgggtcgct tcccgcgatt gacgatgaca
     4081 agtggatcca gattgcccgt tccaaccggg tcgcccccat catggtcatt accaacatcc
     4141 ggcccggcgg cggtttcagc accgaaatcg cccacgccat tctgagcaac caaaccgttc
     4201 aaaacaacct gatcgagaat gttgtccgga ttttaaagca aaagaactat tacggcctga
     4261 atatcgattt tgaatatatc cagcccacag accgccagaa ttacaacgat tttttaagaa
     4321 agataaccga ccggcttcac gccctcggct acattgttct taccgccgtc gccccgaaac
     4381 tgaacgccga gcagaaaggc ctgctttatg aggcgcacga ttatcccgct cacggttcta
     4441 tcgtcgatcg ggtaatcctg atgacatacg agtggggata tctcgccggg ccgccacagg
     4501 ccgttgcgcc cctgagccag gtaaaacggg ttttggatta cgccgtctcg gtgattcctc
     4561 ccaaaaagat tctcatggga atccccaatt acgcctatga ctgggttctt ccctatgtac
     4621 gggggacact tgcctcaacc ttctccaacg gtgaagccgt ggcccgggca ttccgcaaca
     4681 aggccgagat caaatatgac ctcaacgccc agtcccctta ctataactac tatgacgcgc
     4741 agaagcgaca gcatatcgac tggtttgaag acgccagaag catctatcag aaacttctga
     4801 cggtcgatca gtacgggctg ggcggagtga gctactggac gatcggacgc agtttccaac
     4861 agaacctgtt tctggtcgac gccatgttta atattaaaaa agtaatttga tcaatcaccg
     4921 ccggcggaaa attccgccgg ttttttattt catgtcccca aaaccagtct aacccgcaca
     4981 aaaatccaat aaaatataac aggctgcaga gacaaacagt aaaggatgag ttgattggaa
     5041 aagtatgttg tgaccatcgg cgcgggtcac ggggggcacg actgcggggt cgtgggcccc
     5101 tctggggtca tggaaaaaga cgccgcgctg aaccttagtc tgagaacagc ggagctgatg
     5161 aattccaccg gctgtctgag ggccattcct atccgcagcg gcgacgttta cattgatacc
     5221 gcgagacgag cggaaatcat tgcggacagc ggaagccgat gccatattga gctgcatttt
     5281 aacgctcttg actcccgggc cgactacacg gaagtctggc attcgttcgc gcaggcggct
     5341 gaccgggctc atgccgaata tctgtcccgg cggatcgctc aggtacttga tatccgggac
     5401 ggcggaccaa aggcccgaat tgattacggc gcgctgttgg gccggaccat attgcacgag
     5461 gattatctcg gcctgtttga actccttgaa aagcgacgga ttccccatgt ttttctggcg
     5521 gaatgcggct gtattgatca tataacaact gaacaaaaaa tcaacgattt atactatatg
     5581 gatagaatcg ctcaggcaat tacgcaaaca gtctgtgaga ttttttcgtt gagcggtttg
     5641 aaactccgcc tttcaaagca acagcagcca cagattattt ccccaaaggc ggaaaccgtc
     5701 tttttaaaaa acgggtcatt tcaggtcttt tccgggcccg gcccatacta ttccaccgtc
     5761 agaataatcc gcggactgat ttatactaga tgttatcaat cattaaacgg atggatcaaa
     5821 atatccccgt cctcggaaga atggatcagc gcgacggcca taaagaactt tctcgaccga
     5881 aaaaatgtta ttttgcctga ttgtgaagcc acagccgaaa gggttggaaa agccggattt
     5941 ttttatgtca aatcagcacc tgacgtccgt tctgatgttt tgggcgcggt aacgggcggt
     6001 gaacgatgga caaccatcag cgaaaacggc tggagaaaaa tttgttttga tggacgaatc
     6061 ggttatgtcg gagcataccc ctggcaggaa taacctccac aaaaaaaggc cgcttttcgc
     6121 ggcctttcca gtttgcggtc atatctgatt ttactaagtg aaatattcga atcatatcca
     6181 cggggatacc ccgttaactc ttcttatttg ctgataacat cgagccactg gctatcatag
     6241 aggtcaagaa tattctgcgg aagatttgtg taaacctccg ccttttttag gattgcttcg
     6301 gccgggtagg caattgtatt gctccgaatc tcagcaggca gctttttcag cgctgctgtg
     6361 ctgggtgtcg aatatccggt ttcctcacag tttttcacgg atatatcggg tgagcagagg
     6421 aaattgatgt atttctccgc ctcggtttta tgtccggaag ttttcgggac acacatggcg
     6481 tcaacaaaca gatttgtgcc ttcttttggc acggcaaaag caagatccgc gttatcctgc
     6541 atcatcgtaa cggcgtcgcc ggcgtaatac ggcgcgagag cagcctcccc gccttccatc
     6601 ttatcaaaaa tctgatccat gacataagcc tgaaccagcg gcttttgatt gatcagctcg
     6661 gcagccgcct tttttatctg tgcctgatcg gtggtattgt aggaatagcc cagtttttta
     6721 agggcgattc ctattgcatc gcgagaattg tcaaacatga gaattttgcc tttgtatttt
     6781 gaattccaga gaatatccca gctgtcaacc gggtcgctga ccatagtttt gttataaaca
     6841 atacccactg tgccccacat atacggcaca ctgtattcat ttttggcgtc atatttcaaa
     6901 tttaaataat ttttcccaat atctttaaaa ttggggatgt tggagaaatt cagcttataa
     6961 agcatatttt cgctggaaag ccggctaacc atgtaatcgg aagggataat gacatcataa
     7021 tctacgccgc cgcttttgag cttcgcgtaa agttcctcat tggtagcgta ggttttataa
     7081 ttaacattaa taccggtctg cttggtaaac tccttgttaa ccgactcatc aatatattcg
     7141 ccccaattat aaacattcaa tgtgaccgaa gatgtttttc ctccggattt gcacgatgcc
     7201 attggcagaa cacaggccac ggcaagaacc acgcaaacaa ataatttaaa tttcatctct
     7261 gatttccttt ctctgttgtg aattttcccc gcctgcctgc cggacgttga taataattaa
     7321 aaacagcagc acaaacagga acaacaaggt tgacaacgcg ttgatttcag ggcttactct
     7381 ttttctcgtc attgcataaa ttgcgattga gagtgtctgg gaattgtctc cgctcgtaaa
     7441 atagctgatg acgaaatcat cgaccgagag ggtaaacgcc agaagcatgc ccgtgatgat
     7501 tccgggcctg atctgtggga gaaccacctt tcggaaggcg gttgcgggat gggcgccgag
     7561 atcctgcgcc gcctcatata aatgagggtc aagctggcgc agcttgggca taacggaaaa
     7621 aatcacatac ggtgtgtcaa aactgacatg cgccagcagc agcgtaccga aattcagcga
     7681 accgcacgcc cagacaaaca aaagcatgag cgagacgccc atgatgattt cagggcttag
     7741 aattggaaga taggtcacat tgacaaaaac cgtacgcatc cgtttcttca tcgccatgat
     7801 accgatagcc gccgccgtgc ccaaagccgt cgcaatcagg gcggccagaa cagatacggc
     7861 caacgttaca taaacagagt taagaatcat ctggtcggaa aaaagctttc cgtaccactt
     7921 aagcgtgaac cctccccaga cagtgcggct tttggatgca ttaaacgaat aaaaaatcag
     7981 cacaaagatc ggaagataga aaaaagcaag cacaagataa agataaaacc ggttcaggaa
     8041 tcgacgggag gtcttcatat gaccattacc tccttgtcat cgcccttctg gaagcccatc
     8101 gcccacatgc tgatgaggat gatgatcatc ataacaaggc tgatagcggc gccaaggtgg
     8161 ggattgtagg cgttgcccag atattccatt tcaatcaggt cgccgatcag aaggttggag
     8221 cctccgccga gcatcttgga aatgatgaaa gtgcttacgg aaggcataaa gaccataatg
     8281 acacccgaca ttacgcccgg catggaaagc ggaaaaacca ctttcaaaaa gcaccttcgg
     8341 ggtgtggctc caagatcctg ggccgcctca atcaggcggt ggtcgagctt gaccagcacc
     8401 gagtagatcg gcagaatcat ataaggcatg taattataca ccattcccaa tacgaccgct
     8461 ccctgggtat tgatcatatg cagcggcccg attccaaaaa taccgaggaa ccggttgatc
     8521 aggccgttat tttccaaaag cgtcagccac gcgtaggttc ttaaaagaaa gttcatccac
     8581 atcggcagca taatcagcat cagcatcgtg ttttgccttt tcgcaggctc ccttgagaga
     8641 atatacgcca ccggataccc gattataagg ctgagcaggg tggatatcag ggctaaaacc
     8701 accgaacgaa cgaagaccgt cgtatagatt cccatctgtt caaagttcgc aaacgtcagg
     8761 tgattcgacc ggtcggtgac agcgaacccg atcaccagta tcaacggaac caccgtaaac
     8821 aggatactcc agacaagata agggagcgcg gataaatgag cttttctcat gaagccttct
     8881 cacccccgga cttgtgcata atatgtatgt cgttcggatc aatatgaagg ctgacgtttg
     8941 acccggccga ctcactgacc gtggaatgga tcagccagga aaacccctcg ctctccactt
     9001 ccatttcata atgaacgcct ttaaaaatca cggaacggac aatacctttg agctgtgcgt
     9061 gttcttcgcc tgtaattttt acatcctcgg gccggatgac cacatccacc ggctcgtcaa
     9121 tttcaaagcc tttgtcgaca cagatgaaat tcctgcccgc aaactccacc aggaaatcct
     9181 catgcatcac gccggggaca atgttgcttt cgccgataaa atcagcgaca aaagcattgg
     9241 ccggctcatt ataaatatcc tgcggagtgc ctgactgcaa aatattcccc gcgttcatca
     9301 ccaccacggt gtcggacatg gtcagagcct cttcctgatc gtgagtaaca tagataaacg
     9361 taattcccag gctctgctga atctttttta gttccagctg catttctttc ctcagtttca
     9421 gatccagcgc gcccagcggt tcgtcaagca gcaaaacgcg cggctgattg accagagctc
     9481 ttgcgatggc caccctttgc tgctggccgc cggaaagcga atcaatccgt cttgtgccga
     9541 atccatcgag gttgaccagc ttgagcattt catccgtttt ttctttaatt ctcgctttcg
     9601 gctccttttt cacattcagg ccaaacgcga tattttcatt aacattgaga tgcggaaaca
     9661 gagcatatct ctggaatatg gtgttgacct gccttttata gggggggcag atcattgatg
     9721 cgccggtctt caaaaaaaac gtcgccgcca tccggagagg taaacccgcc gatgattcgc
     9781 agcgttgtgg ttttaccgca tccggaggga cccaacaatg taacaaattc cttatctttt
     9841 atgttgatat tgattttttt taatactttc acaccgtcga aagccactgt aatatccttc
     9901 agtccgacaa tgcagttttc actcatcgac aatcaccttc caatcgtatc aaatcacaat
     9961 ataatgatta tgagggtaaa agtcaatata ttttgcgaaa aaacgtgatt aatcatgaaa
    10021 tttttatgat tttttaattc atcatataaa ctattcaggt tttctttcat aaactgtgtt
    10081 tcatattaat tctcaccagt tccaaaccac aatattcaag aacctgtcca catcactttt
    10141 gaccgtcggt ttaagaatcg tctaaaacag cagtccgacc cgctcaaaat acgaaaagcg
    10201 gccgcctttt tataagacgg ccgtataaca cttctcaccc tattgactga tcatgagtta
    10261 tattcagcga caatttcttt cagtgcggct ttacacccgg catcctctcc attgtccgcg
    10321 accattttga gtgtttttag atcctcctcc aaatttcggg ccaatcggga ggatttgcag
    10381 acaaaaatgc ggtcgtagac tgttttctga acgtcctcat cgtccagcga aagttcctca
    10441 tacatttttt cgcccggccg caggccggta aattcgattt taatatcttt ctgcggctca
    10501 aaacccgaca tccggatcat ttccgccgcc agatcggcta tctttaccgg ttcccccata
    10561 tccagaacaa aaatttcacc gccccgggtt aactccccgg cttgcatgac aagctgaacc
    10621 gcctccggta tggtcataaa aaatcttctg atatccgggt gcgtaaccgt cacgggcccg
    10681 ccatgcatga tctgacgctt aaacaggggg acaacgctcc cgttgcttcc gagaacattg
    10741 ccgaagcgaa cggccgcaag cctcgtgaat tttgagccaa cattcagatg ctccacaatc
    10801 atctcggcca cgcgtttcgt tgcccccatt acgtttgtgg gcttgaccgc tttgtcggta
    10861 gaaatcagga taaacttttc ggctcggtag cgtatggcaa accgggcaac gttaaaagtt
    10921 ccaaacacat tatttttgac agcttccgcc ggattcgact ccatcagagg aacatgcttg
    10981 tgcgcagcgg catgaaaaat cacgtcgggc ctgtattctg caaaaaggcg gtcaagactg
    11041 ccagcgtcac gcaccgatcc gacaacgatc tgatatttct gacgcggata ggtttttttt
    11101 aaatccgcgt ctatttcaaa caggccgttt tcatgaatat cgaagatcac caatttggcg
    11161 catccgtatt ccaaagactg cctgcagatt tctgagccga ttgaccccgc cccgcctgtt
    11221 accaacacgg ttttgttctt gatcacccgg cgggcaggca gagtgaattc ggtctggact
    11281 cttccgagga ggtcacccgt ctggattttc tccacccggt tgcggatgcc gtcatacttt
    11341 tggatacctg agtcaacaag caagcggcac cggttttcag aacaaatcct aacgatgtca
    11401 agcagcttgc tttcaagcag cccgttcctt gacgccacaa cttcatccgc ttgcatcagc
    11461 cgaatagaac tctccacact tttaaggccg cagccataaa ctcttactcc gctgatgtaa
    11521 tagccggact catgcgactc atccgggtcc accactgcaa ccacggtttt ttcccggttg
    11581 tccgcccgta ttttttcaat cagcgccttg ccgtcaagag gccccgctac gaccagaacc
    11641 tttgagtggg ccggatgccg gctccatcgc ctgaagcgcg cggatgcggc aagaaagccc
    11701 cgataggaat agcgaaaaaa agccatcaga accatctcga gcagcacgca ggataaa
//
LOCUS       DATALY010000269         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_489281,
            whole genome shotgun sequence.
ACCESSION   DATALY010000269 DATALY010000000
VERSION     DATALY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489281"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VHR42_07405"
     CDS             <1..879
                     /locus_tag="VHR42_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX3027037.1"
                     /translation="LFGGERIQNLMGTLGIEENQPIENRMLTSSIENAQRKVESKNFG
                     IRKSVLQFDDVMNRQREIIYGQRRRVLDGENLRDYIVGMVKESVEKSFAQFIGDDPIA
                     DNWNLSGLRDFYLGWITREDDFKYTRDELQELDKEDIKETLLERATGKYEERERRLGE
                     DIMRELERVILLRTVDEKWMDHIDAMEELRQGIYLRAYGQRDPVVEYRIEGFEMFDEM
                     ISSIQEDTVRLVLTVQVRSEEEPKREQVAKPTVASHGEGEEKRQPVRIAKKPGRNDPC
                     PCGSGKKYKKCCGMNE"
     gene            1022..1990
                     /locus_tag="VHR42_07410"
     CDS             1022..1990
                     /locus_tag="VHR42_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3027038.1"
                     /translation="MYQKGSVKVNTVYFVLPCYNEEAVLPETTRRLTEELRGLIDSGR
                     AGEKSRMLFVDDGSRDKTWEIISRLSEENPFVCGLKLSRNCGHQNALLAGLSTARDFA
                     DCAISLDADLQDDISVISEFVDKYQQGYEVVYGVRKNRDTDTSFKRVTAESYYKFLRI
                     LGVNVVDNHADYRLMSKRAIESLLEFREVNLFLRGIVPLVGFASVNVYYDRQERFAGE
                     SKYPLKKMLAFAVDGITSFSVVPLRIITAIGVFVFVLSVAALIYALVAKLTGNAEAGW
                     ATIVGSIWLIGGIQLLCLGICGEYIGKIYKESKHRPRYMIDRAVIK"
     gene            complement(2043..2804)
                     /locus_tag="VHR42_07415"
     CDS             complement(2043..2804)
                     /locus_tag="VHR42_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3027039.1"
                     /translation="MKSSSDSQFESILSENRLHTLFQPIVNFKDGKTAGFEALSRGPD
                     HTAYFSPLFLLEEAAQSGKTRDLDRLFRKNALERAREQQIAEPLFLNIEPDSIVEFGR
                     DETEFRSDRRLVLEITERAVHSRRKDLPEAQLRHMRSVGYRLAFDDAGTGHSSYDRMI
                     QIKPEFIKIDAGLIQGIHRDRYRQSLIQSFVLFSRLTGSLLIAEGIETLSDLETLVRL
                     GVQAGQGYLLGRPERYAVGPRQNIIRQVRQMSKLC"
BASE COUNT          784 a          699 c          820 g          734 t
ORIGIN      
        1 ctcttcggcg gggaaagaat tcaaaatctg atgggcacac tcggtatcga ggaaaaccag
       61 cccatcgaga atcgcatgct gacaagctcg attgagaatg cccagcgaaa agtcgaaagc
      121 aaaaacttcg gcatccgcaa aagtgtgctt cagtttgacg acgtcatgaa ccgccagcgt
      181 gaaattatct atggccagcg gcgccgcgta ctcgacgggg agaacctgag ggattatatt
      241 gtcggaatgg tcaaagagag cgtcgaaaaa tcattcgctc agtttatcgg cgacgatccc
      301 attgcggaca attggaattt gagtgggctt cgtgattttt atctcgggtg gattacccgt
      361 gaggacgatt ttaaatacac ccgcgacgag ctgcaggagc ttgataaaga agacatcaaa
      421 gaaacactgc tcgagagggc taccgggaaa tatgaggaga gggaacgccg gctcggcgaa
      481 gacattatgc gggagcttga acgcgtcatt cttctcagaa cggtcgacga aaagtggatg
      541 gatcatatcg acgcaatgga ggaacttcgg cagggcattt atctgagggc ttatggtcag
      601 cgcgatcccg tcgttgaata ccgcatcgaa ggctttgaaa tgtttgacga gatgatcagc
      661 tccattcagg aagataccgt ccgtctagtg ctcactgtgc aggtcaggag cgaagaggag
      721 ccgaaacggg agcaggttgc caaacctacc gttgcaagcc atggggaggg cgaagagaaa
      781 cgccagcccg tccgaatagc aaagaagccc gggcgcaatg atccctgccc ctgcggcagc
      841 ggaaagaaat ataaaaagtg ttgcggtatg aacgaatagc gatttgcttt gcgggaaaag
      901 agccgaatac cggtcggcct gctttataaa tgaggatacc ggggggaaag ggacgatcat
      961 tgtccatctc cctttttgtt tttcttgtcc caggtaaaaa tcaagatttg catcagatgc
     1021 tatgtatcaa aaaggaagtg ttaaagtgaa tacagtctat tttgttttac cctgctataa
     1081 tgaggaagct gttttaccgg agaccacccg aaggcttacg gaagaactgc gcggcctgat
     1141 tgattccggc agggccgggg aaaagagccg aatgctgttt gttgacgacg gaagccgtga
     1201 taaaacatgg gagatcatct cccggctcag cgaggagaac ccgttcgttt gcgggctcaa
     1261 gctttcccga aactgcggtc atcaaaacgc tttgctcgcg ggcttatcaa cagcacggga
     1321 ttttgcggac tgtgccattt cgcttgacgc cgatttgcag gacgatatct cggtgatcag
     1381 cgaatttgtt gataaatatc aacagggcta tgaagtggtt tatggtgtca gaaagaacag
     1441 ggacaccgac acatccttta aacgtgtcac agcggagagc tactataaat ttctgagaat
     1501 cctcggtgtc aatgttgttg acaatcacgc cgattaccgg ttgatgagca aacgggccat
     1561 tgaatccctg cttgaattca gggaagtcaa tctgttttta agaggaatcg ttcctttggt
     1621 cggctttgcc tccgtcaatg tctattatga ccggcaggag cggtttgccg gcgaatcaaa
     1681 atatccgctt aaaaaaatgc tcgcctttgc ggtggacggc attacctcct ttagcgtagt
     1741 tccccttagg attattaccg ccattggcgt ttttgttttt gtgctgagtg tagccgcatt
     1801 gatttatgct ctggttgcaa aactgacagg caacgcggag gccggctggg ccacaattgt
     1861 cggctccatc tggctgattg gaggcataca gctgctatgc ctcggcatct gcggggaata
     1921 catcggaaag atttataagg aatccaagca ccgaccgcgc tatatgattg accgggctgt
     1981 aataaaatag aattggaaat cttcgtttta tgaaaggccg cttcgccatc gcgaggcggc
     2041 ctttagcaaa gcttgctcat ctgtctgacc tggcggataa tattctgccg tggccccaca
     2101 gcgtatcttt cgggtcgccc gagaagatat ccctgccccg cctgcacgcc gagacggaca
     2161 agcgtctcca ggtccgacag ggtttcaatc ccctccgcga tgagaagcga accagtcaga
     2221 cgggagaaca gcacaaaaga ctggatgagc gactgacggt aacggtctcg gtgaattcct
     2281 tggatcagtc cggcgtcaat tttaataaat tccggtttga tctgaatcat ccggtcgtag
     2341 gaggaatgac ccgttcccgc gtcatcaaag gccagccggt aaccgacaga gcgcatatgt
     2401 ctgagttggg cctccggcag gtcttttctg cgggagtgga cggctcgttc ggtgatttcc
     2461 agcacaagcc tgcgatcgct gcggaattcg gtttcgtccc ggccaaattc cacaatggag
     2521 tcaggctcga tatttagaaa aagcggctcc gctatttgct gctcccgggc acgttccaga
     2581 gcgttttttc gaaacagccg gtcaagatcc cgggttttcc cagactgggc agcttcctca
     2641 agcagaaaga gcggagaaaa atacgccgtg tgatcaggcc cgcggctcaa tgcctcaaat
     2701 ccggctgttt ttccgtcttt gaaattgacg atgggctgga acagtgtatg aaggcgattt
     2761 tcactgagaa tggattcaaa ctgtgaatct gatgatgatt tcatggttgc tctcctgttt
     2821 ttcacttagc cgattgcatc gtcaatattt attcttcctc agattgtctg aaatactcaa
     2881 aagaacaatc agaaaaaata ataaggtctg ttcccggctg tataagttca tcgtggcgcg
     2941 ccgggcggac gaatgagcca gatgattttt ataaatcagg gttgaaaaat tgaaaattgt
     3001 ggtatataat gtacccatca tcatgtaccg cgactaa
//
LOCUS       DATALY010000270         5739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_489632,
            whole genome shotgun sequence.
ACCESSION   DATALY010000270 DATALY010000000
VERSION     DATALY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489632"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..2019
                     /gene="ade"
                     /locus_tag="VHR42_07420"
     CDS             235..2019
                     /gene="ade"
                     /locus_tag="VHR42_07420"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEX3027040.1"
                     /translation="MENPKPLWEVTADLSAVAMGRLPADLVIRNGQLVNVFTGEILEH
                     MDVAVKHGRIALVGDASGCIGKKTRIIEANHDYLVPGFLDGHIHVESSMLTVREYARI
                     TVPHGTSAIFMDPHEIVNVSGLDAMRVMMDDASWTPLRVFTTTPSCVPAAPGLENTGG
                     AVSAEDIEDTMKWSGVAGLGEMMDIFGILDSDPKAHAKVAAVLRAGKPVTGHFALPDT
                     GSMLNAYAAAGISSCHESTRPEEALAKLRLGMYAMVREGSAWDDLPTVIKAVTQSGID
                     TRRVVFVSDDLHADTILSCGHMDAIARLAIQCGVEPVTAIQMITINTAECFGLSGEIG
                     SIAPGRLADINILSALKSVRVRQVIIGGEPVAENGELCTGLTSVRYPEFLRTTMKINR
                     VVQADDFAVPIPGNVDYKGVRVMEIHENNVLTSSIIQTLPVENGMVQSDPEQDICKVA
                     VINRHGLDSMFVGFVKGFSLKKGAAASTYAHDAHNLIIVGKNEEDMALAANTLVSCGG
                     GMTAVADGEVLQCVELPVAGLISEKSASEVAGELKDLAGAWKEFGCPIESPFMTMSLL
                     SLAVIPELRITDKGMVDVLKNKLVSLFV"
     gene            complement(2159..3049)
                     /gene="eutC"
                     /locus_tag="VHR42_07425"
     CDS             complement(2159..3049)
                     /gene="eutC"
                     /locus_tag="VHR42_07425"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEX3027041.1"
                     /translation="MSEETLKKIVKSVLSQMDNSPAETKRPADQAASQTAPVPDGCGE
                     CLEDITTPEYSEKILVKEPKDLNSLKLFKASTPARIAVGRTGDRPLTESFIRFRADHA
                     AAIDSVFSEVDEDLIDELKLIKLKTRVGNKDEHLTRPDYGRELDEKSIEALKENGVKN
                     PEVQVIVSDGLSSKAIEANIRDVLPAFLQGLSVYHIKVGTPVFVKYGRVGVMDAIGEE
                     LGATAAILFIGERPGLVTAESMSAYMVYHPKRGMEEAERNVVSNIHAGGTPPVEAGAH
                     IATLMKRILDEKASGINMKK"
     gene            complement(3076..4440)
                     /locus_tag="VHR42_07430"
     CDS             complement(3076..4440)
                     /locus_tag="VHR42_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEX3027042.1"
                     /translation="MNLKARVYGKTYTFHTVKEVLAKANEEKSGDILAGVAAASGEER
                     VAAQQVLSCLTLEDLRNNPVVPYDEDEVTRIIQDSVNERIYGEIKNLSVAELREKLLS
                     HHTSGEDIKRISRGLTSEMVAAVAKLMSNLDLITAAKKIRITAKCNTTIGGEGKLAVR
                     LQPNHTTDDVEGMLASLREGLTYGAGDAVIGINPVEDTVENVTRLLEATHHFMTEWDI
                     PTQNCILAHITTQMKAIEKGAPSDMIFQSIAGSEKGNRAFGISIDLIRQAHDMAMKLG
                     TSAGPNVMYFETGQGSELSSNAHYGADQVVMEARCYGLAKQFNPFLVNTVVGFIGPEY
                     LYNSKQVIRAGLEDHFMGKLTGLPMGCDVCYTNHMKADQNDIENLAILLAAAGCNYFI
                     GVPMGVDVMLNYQCTSFHDNAAVRSLLNLRPLPEFEKWLEKMGIMCDGRLTNRAGDAS
                     IFTR"
     gene            complement(4456..>5739)
                     /locus_tag="VHR42_07435"
     CDS             complement(4456..>5739)
                     /locus_tag="VHR42_07435"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX3027043.1"
                     /translation="VVDHHLIDVSELRRIIEKEYQKAGFSAKAIDTGAIIITGETAKK
                     ENAEKILHELAGFAGDFVVAAAGPELESILAGKGSGAAELSKKTGKTIINLDIGGGTT
                     NIAVFECGRVADAICVNVGGRLIEIDAESHEIAYISRPAQLFLDYCRINLKKGDRADQ
                     SQIETVCKAMAEYVGQIALGEETPSALSEILMTAPLKRNYRYDGVMFSGGVAEYIYLK
                     NAAHSQDYAYGDMGPVLARAFQNCPLAGDYNLISPDQAIRATVVGAGTQTVNLSGSTI
                     FIDKSILPVRNIPVAKVDWRQFPEREEDLSDLICGAIRRYQSSDHKGILAVSMPCPQP
                     LSFSNISRMAGGIYDAWKKTIVPAPLVIILEKDIGKVLGQTLYTLSDGKMKFLSIDSV
                     SLSDGDYIDIGKPISADDVVPVMIKTLIFSESGVN"
BASE COUNT         1320 a         1426 c         1491 g         1502 t
ORIGIN      
        1 gactcggcta atccgcggta tggcgatagt aactgattct cggcttgagc ggcgtttcat
       61 ggttggtatc gggaataaga ggatggaaca gtttttgcaa ttgacaaatt aagggtcaaa
      121 ggataaactg aatgaaatac atgttcgttt aatttcccgc aggaattatg accgtaacat
      181 aggccataac tcacttaaag tacgagagga aaaaaagtca gggaggtatg gagcatggaa
      241 aatccgaaac cactttggga agtaacggca gatcttagcg ctgtcgccat gggccgcctg
      301 ccggcggatc ttgtgatccg taacggacag cttgtcaacg tcttcaccgg agagatattg
      361 gagcatatgg atgtggcggt caaacatggc cgtattgcgc tggttggaga cgcgagcggc
      421 tgtatcggaa agaaaacacg gattattgag gcaaatcatg attatcttgt tccgggcttt
      481 cttgatggcc atattcatgt tgaaagcagt atgctgacgg tgcgggaata cgcgaggatt
      541 accgtccctc atggcaccag cgcaattttt atggatccgc atgagattgt caatgtttca
      601 gggctggatg caatgcgcgt gatgatggat gacgcaagtt ggactccgct tcgcgttttc
      661 accacgactc cttcctgtgt ccctgctgcg ccgggcctgg aaaatacagg cggtgccgta
      721 tcggccgaag acatcgaaga taccatgaaa tggagcggcg tggccgggct tggagaaatg
      781 atggatatct ttgggattct cgacagtgat cccaaggctc acgcgaaagt tgcggcagtc
      841 cttcgggcgg gcaaaccggt aacaggtcat ttcgcactgc ccgatacggg gtcgatgctc
      901 aatgcttatg ccgcggccgg aatcagcagc tgtcatgaat ccacccgtcc ggaggaggcg
      961 cttgcaaaac tgcggctggg gatgtacgca atggtgcgcg aaggctctgc ctgggatgat
     1021 ttgccgacgg ttatcaaggc ggttacccaa agcgggattg atacgcgccg ggtggttttc
     1081 gtatcggacg atctgcatgc ggacacgatt ttatcttgtg gccatatgga tgcaatcgcg
     1141 cgtcttgcca tccagtgcgg agtggagccg gtcacggcaa tccagatgat tacaattaat
     1201 acggcggaat gcttcgggct gtccggtgaa atcggctcga ttgcgccagg acgcctggcg
     1261 gatattaata ttctcagtgc gctcaaaagt gttcgggttc gtcaggtgat catcggcggg
     1321 gagccggttg ccgagaacgg agaactttgc acggggctta cgagcgtcag atatccggaa
     1381 tttctccgaa caacaatgaa aatcaaccgg gttgttcaag cggatgactt tgcagttccg
     1441 attccgggaa atgttgatta taagggcgta cgggtgatgg agatccatga aaacaatgtg
     1501 ctcacctcat ccattataca aactctcccg gttgagaacg gaatggtcca gtcagatccc
     1561 gagcaggata tctgtaaagt cgcggttata aacagacatg gcttggacag catgtttgtt
     1621 ggattcgtaa agggattttc gttaaagaag ggggctgcgg cgtcgaccta tgcccatgat
     1681 gcccacaatc tgatcattgt cggtaaaaat gaggaagata tggcattggc cgccaatacg
     1741 ctggtttcct gcggcggagg aatgacggcg gtagcggacg gagaagtcct ccaatgtgtg
     1801 gaactgccgg tggccggcct gatcagtgaa aaatcggcgt ctgaagttgc cggggaactg
     1861 aaagaccttg ccggagcatg gaaagaattc ggatgcccga ttgaatcccc gttcatgacg
     1921 atgtcgctgc tttcgctggc tgtgatacct gaacttcgga tcacagataa aggcatggtg
     1981 gatgttttga agaacaaact ggtaagcctg tttgtctagc ttaccggcac gcaaacccgc
     2041 gttcagggat aaaatgaaaa aagccttaca gctcaaataa agctgtaagg cttttcctga
     2101 ccccctttaa gggggggagt atttgcggcg ggttttatcc gttagcagga gcgcgaggtt
     2161 actttttcat gttgatgccg ctggcttttt cgtccagaat ccgtttcatc agggtcgcaa
     2221 tatgcgctcc cgcttccacc ggaggagttc ctccggcgtg gatgttggaa actacgttgc
     2281 gctcggcctc ttccattccg cgcttcggat gataaaccat ataggcgctc atgctctcgg
     2341 cagtaaccag acccggccgt tctccgatga aaagaatggc agcagttgct ccgagctctt
     2401 cgccgatggc gtccattaca ccgactcttc catatttaac aaaaaccggg gttcccactt
     2461 tgatatgata aacgctcagc ccctgcagaa aagcgggaag aacgtcccgg atattcgcct
     2521 cgatcgcctt ggagctaagc ccatcggaga caataacctg aacctccgga tttttgacac
     2581 cgttctcttt cagggcctcg attgattttt catccagctc tctgccataa tccggtcttg
     2641 tcagatgttc gtctttattg ccgaccctgg tttttagctt gatcaatttt agttcgtcaa
     2701 tcagatcctc atcaacttca ctgaaaactg aatcgattgc ggcggcatga tcggcacgga
     2761 accggataaa agattcggtc agaggccggt cgccggtgcg cccaaccgcg atcctggccg
     2821 gggtcgacgc tttaaaaagt ttgagagaat ttaaatcctt tggctctttg accaatattt
     2881 tttcggaata ttccggtgtg gtaatatctt caaggcattc tccgcagcca tccggaacag
     2941 gggcggtctg acttgccgcc tggtctgccg gccgtttggt ctcggccgga gagttgtcca
     3001 tttgactgag cactgatttt acgatctttt ttagtgtttc ttcgctgata ttcatacgat
     3061 caccccttca cccgattatc tcgtaaatat tgacgcgtca ccggccctgt tggtcagtct
     3121 gccatcacac atgatgccca ttttttccag ccacttttca aattccggaa ggggcctgag
     3181 attcaaaagt gaacggacag cggcgttgtc atggaaactg gtgcactggt agttcagcat
     3241 tacgtccacc cccattggca cgccgataaa atagttgcat ccggcggcgg cgagaagaat
     3301 agccagattt tcaatgtcat tctggtcggc cttcatatgg ttggtataac agacgtcgca
     3361 gcccatgggc aggccggtca gtttgcccat aaaatggtct tccaatcccg cccgaatcac
     3421 ctgcttgctg ttatacagat attcggggcc gataaacccg acaaccgtat tgaccagaaa
     3481 cgggttgaac tgtttggcaa gcccgtaaca cctggcttcc ataaccacct gatcggctcc
     3541 gtaatgggcg tttgaagaaa gctccgatcc ttggccggtt tcaaaataca ttacgttcgg
     3601 cccggcggat gtccccagtt tcatagccat gtcatgggcc tgacgaatca ggtcaatgga
     3661 aattccgaat gcccggtttc ctttttccga gccggcaata ctctgaaaga tcatatccga
     3721 aggggcgccc ttttcgattg ccttcatctg ggttgtgatg tgggcaagga tacagttctg
     3781 ggtgggaata tcccattcgg tcataaaatg atgcgttgct tcaagcagcc gggttacgtt
     3841 ttcaaccgtg tcttccacag ggttgatccc gatcaccgcg tcgcccgcgc cataggtcag
     3901 gccttccctc agcgatgcga gcatcccttc cacatcgtct gtggtgtggt ttggctggag
     3961 tctgacggca agttttcctt ctccgccgat tgtggtgttg catttggcag tgatccggat
     4021 tttttttgcc gcggtaatca aatcgagatt tgacatcagt ttggcaacgg cagccaccat
     4081 ttcgctggtg agtccgcggc tgattctttt gatgtcttca ccggaagtgt gatggcttag
     4141 cagcttttca cgcagctccg ctacagaaag gtttttgatt tcaccgtaga ttcgttcgtt
     4201 tacggaatcc tgaatgatcc gggttacttc atcctcgtca tacggcacaa ccgggttgtt
     4261 ccttaaatct tcaagagtaa gacagcttag gacttgctgc gcggcgaccc gttcttcacc
     4321 cgaagcagcg gcgacgcctg ccagaatgtc tcccgatttc tcttcattgg ctttggccag
     4381 aacttctttg accgtgtgga atgtataggt ttttccgtat actcttgctt tcagattcac
     4441 ggaacgattc ctccttcagt tgactccgct ttcagaaaaa atgagcgtct tgatcataac
     4501 aggcacgacg tcatccgcag aaatcggttt gccgatgtca atataatctc cgtcgctcag
     4561 gctgaccgag tcaatgctta aaaatttcat tttcccgtcg gagagggtat agagcgtctg
     4621 tccaaggact ttgccgatat ccttttcaag aatgatgacc agcggagcgg gaacaatcgt
     4681 ctttttccag gcgtcgtata ttcctccggc catccggctg atgtttgaaa aggacaatgg
     4741 ttgggggcag ggcatggata cggcaagaat ccccttgtga tcggaactct gatatctccg
     4801 tatcgcgccg cagataagat cggaaagatc ctcttcgcgt tccggaaact gtctccaatc
     4861 cactttggca acgggaatat tccgaaccgg caggattgac ttgtcaatga atatcgtgct
     4921 gccgcttaga ttgaccgttt gtgttccggc cccaacaaca gtggcccgaa tggcctgatc
     4981 tggggatatc agattgtaat ccccggcgag cgggcaattt tgaaaagccc gggccagcac
     5041 cggtcccata tcgccgtagg cataatcctg cgaatgagcg gcgtttttta aataaatata
     5101 ctccgctaca ccgccggaaa acatgacgcc gtcatatcgg taatttcttt taagtggagc
     5161 cgtcattaaa atttcggaca gagcggaagg agtttcctct ccaagcgcaa tttggccgac
     5221 atattccgcc atcgctttgc aaaccgtctc aatttggctt tggtccgccc gatctccctt
     5281 tttaaggttt attcggcagt aatccagaaa aagctgcgca ggcctcgaaa tataagcgat
     5341 ctcatggctt tcggcatcaa tttcaatcag tctgccgccg acatttacgc agattgcatc
     5401 tgcaactctg ccgcattcaa acacggcaat attggtagtg cctccgccaa tatccaaatt
     5461 tataatggtt tttcctgttt tttttgacag ctcagccgcg ccggatcctt tcccggcaag
     5521 aatggattcc agctcaggtc cagctgccgc gacgacaaaa tccccggcaa accctgcaag
     5581 ctcatgaagt attttctccg cattttcctt tttagcggtt tcgccggtta taataatggc
     5641 gcctgtatcg atggccttcg cgctgaagcc ggccttttga tattcttttt caataatcct
     5701 tcttaactcc gatacatcta tcagatgatg gtcgactac
//
LOCUS       DATALY010000271         8606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_145554,
            whole genome shotgun sequence.
ACCESSION   DATALY010000271 DATALY010000000
VERSION     DATALY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145554"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..814
                     /locus_tag="VHR42_07440"
     CDS             215..814
                     /locus_tag="VHR42_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027044.1"
                     /translation="MVTIQRFNKNLLTDEGYLSFTLPVKTHCHVDCKMIVSLIWFMLS
                     GIVATVSIFVLAIDANTLVHLQEFFAECADAFRQVGANGVIVILEGILLLLLSVLSST
                     ITIYAAITIGNMSSKHKLLAGVGAYLGFGVIEQIITTTVLSTLHDWPEHYFDNIKTIQ
                     GGVQAAEIIMLITIIYTLIFGVAYYLLTNWMLKNKLNLE"
     gene            1051..1959
                     /locus_tag="VHR42_07445"
     CDS             1051..1959
                     /locus_tag="VHR42_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027045.1"
                     /translation="MNAISAKGLTKSYDGKALALDSLNLEVPRGGIFGFLGPNGAGKT
                     TTVKLLTGLLKPTKGECRVFDLDPGRKPCDVHRICGVMTESAKMYGQLTGMENLIFFA
                     QTSGMTQEACGLKAGELLKQLDLWDARDKKLSAYSTGMAQRLSLARALINSPQVLFLD
                     EPTSGLDPESAQAVNELIMTLAKNSGTTVFLCTHQLRYAEDLCSSYGIIEKGKLLASG
                     DLQSLCDAIGCRIRAGFRLAPGETLEGFAEEFGWWQTEIAKEEEMPDLLKKIMDDGRH
                     VYEARLIRPTLEDVYFSYTERQEAQQ"
     gene            1956..2753
                     /locus_tag="VHR42_07450"
     CDS             1956..2753
                     /locus_tag="VHR42_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027046.1"
                     /translation="MKLFSTAEKAIMKKDFGEIWDTKMARNTLLLLPIILVVILPAFY
                     LALIYFIPAEQINGADKMLKLLPPEAQGFSVQQSMFYMMTNFLCPMFFLMIPLMVSSI
                     SAACSFVGEKERSTIETLLLTPMSMRQIFKAKVFGCVILSGISTLIAFVAFSVVTSLG
                     DLIMKMPFFLNWNWLVLLLFFAPAITVFGVIFMVIFSGKSKSYMESFQTSSYIVLPVI
                     LLFMGQFTGLFQLNALILLLISAVIIILDIVLLLFGSRSFTPEKLLK"
     gene            2827..3666
                     /locus_tag="VHR42_07455"
     CDS             2827..3666
                     /locus_tag="VHR42_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3027047.1"
                     /translation="MAADLHCHTKMSDGSVSIDEVVMLAKKRGLSTIAITDHDTFAGT
                     TRAKIFGDRHGIEVISGVEFSTMDPATGRKAHILCYLCDNPDRLVGLCKRTADSRRRA
                     ASIMLQKTMRIYPITADMVVRRAHGSTNIFKQHIMHALIDAGYASHFFGATFKKLFNS
                     KNGLAYCSVEYPDVHDVIKQIHDAGGIAVLAHPGEYNSYELLDQLGSSHEIEGVEVWH
                     PRNKPGDEEKFIAAAKKYSLVMTGGTDFHGMYTSVHTPIGTCTTPDDQLEALKKRKAQ
                     MTE"
     gene            3679..3939
                     /locus_tag="VHR42_07460"
     CDS             3679..3939
                     /locus_tag="VHR42_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HEX3027048.1"
                     /translation="MEYHYKTKGTCSSEIMVELDGDTKKAVEIVGGCNGNSKGICSLV
                     QGMKADEVIRRCKGIRCGFKPTSCPDQLANALLHAQKQQSGK"
     gene            complement(3979..4104)
                     /locus_tag="VHR42_07465"
     CDS             complement(3979..4104)
                     /locus_tag="VHR42_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027049.1"
                     /translation="MCNNFFGGGNSCTWVILLIIILVCCCGGNDCDRGCGNNGCC"
     gene            4296..4796
                     /locus_tag="VHR42_07470"
     CDS             4296..4796
                     /locus_tag="VHR42_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX3027050.1"
                     /translation="MLCECCGKNPVTTYVKTIINGELTKYSLCAECAQKLGYGSLLTG
                     LGLNYGSLLGAFFGEPEQEDKDSVRCKCCGCSFEDIARSGRVGCAECYHTFYNRLIPL
                     IQRIHGNTKHRGKVPGGEQLQPRPQSQLSVMRRELREAINSENFEHAANLRDRIRELE
                     GKENRE"
     gene            4789..5838
                     /locus_tag="VHR42_07475"
     CDS             4789..5838
                     /locus_tag="VHR42_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HEX3027051.1"
                     /translation="MSKWYEKSGAEGDVVIGTRIRLVRNLNGFPFPARMSKADRRILL
                     QRVAETVGSCAQAYGFQFLNMEETSKTQAVSLVERYLASPEFISEPDGRGMLLTEDES
                     LSIMLNGENHLTIQTITEGLDLQQAYQMADQLDTILDRSLHFAFDEALGYLTQNPVDL
                     GTGMRASLVLHLPAMQDGGGITRISGSLSKLGLSLRGIYGSGTTSKGAVYELSNQVTL
                     GLSEQEALANLQSIALQIIRQERTARTELGERLDVQDAVSRSLGILQSAKLMTNEEFM
                     GLISNVRFGVAQGLFDKISYSTINKLVIVAQPATLALYSGKKLTANERHLLRAQTVQN
                     LLKADDFYGAANSEQ"
     gene            5813..6463
                     /locus_tag="VHR42_07480"
     CDS             5813..6463
                     /locus_tag="VHR42_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027052.1"
                     /translation="MEQPIPSNEELAAALLWALGENIPHDRALEAEAIRLKAEKASSE
                     ELLQKVIALCSNSKTPMQLYLTAKAYSLLGSGFYSQTIESMQEYLKTGGWDKLSGHSV
                     MENGILINHLCMQRAADLVVLARAQESSGKLEDALESFTEAYRFEPYRAMHAVKVADL
                     LVKLHGREEALLFLRHQKQSRYYSPIKYTDTLGEVRYNDEFRRLLDAHILKIQNIG"
     gene            complement(6564..7943)
                     /locus_tag="VHR42_07485"
     CDS             complement(6564..7943)
                     /locus_tag="VHR42_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3027053.1"
                     /translation="MIKNMKIGNKLVFAFILVAVLSGVGSIVGFFEIRKLGSLSASLS
                     AQASASGMIILAILCASLILSLVIALSIARSISKPVEEMAEAAQRMAEGDLNVQVNYD
                     SQNEIGQLAAAFKESTTSIRAYIADLTEHLDKVAHGNLAMPSKMQYVGDYKGLSIAYH
                     GIISSLSETLSEINEASEQVSNGSEQVSGGAQALAQGAAEQASSVEELSATIVEISSQ
                     VRENAEHAAEASRNVNHVSSEVEISNNHMHEMLGSMSQISESSNEIGKIIKTIEDIAF
                     QTNILALNAAVEAARAGAAGKGFAVVADEVRNLASKSALAAKDTTVLIENSIKQVANG
                     QKIAGETAQSLVRVVDNIKTVTDTINQISEATNHQSEEISQVTTGVEQISSVVQTNSA
                     TAEESAAASEELSGQAKALKTLVKQFKLKDKSVQISETKADEKADNVKSFRPDIPQSQ
                     QTVLADVKY"
BASE COUNT         2229 a         2020 c         2134 g         2223 t
ORIGIN      
        1 actacttata ccaccgcaac cgcccgattc cgggaattat gagaacatta tagttgttcg
       61 ccgtgctttt ttcaactaaa aatacaaaag gcgcgtcctg tgacgcgccc tcaaactgta
      121 tttgcgtaaa gcttagtcta aatttaactt gtttttcagc atccagttgg tcagaagata
      181 atatgcaact ccaaaaatca gcgtgatgac gctggtggtt acgattcagc ggttcaacaa
      241 aaacctgctc accgatgaag ggtacctgtc ctttacttta ccggtcaaga cccactgcca
      301 tgtggactgc aaaatgattg tcagcctgat ttggttcatg ctgagcggaa ttgtcgcaac
      361 agtttccatt tttgtattgg caatcgacgc gaatacgctg gttcatttgc aggaattctt
      421 tgcggaatgt gcggacgcct tccgtcaggt gggagcaaac ggagtaatcg ttattctgga
      481 aggaattctt ctactccttc tttccgtcct ttcgtccacc attacgattt atgccgcaat
      541 cacaatcggc aacatgagct ccaaacataa acttttagcc ggtgtaggcg cttatcttgg
      601 ttttggggtt attgaacaga tcattaccac aaccgttctc agcacattac acgactggcc
      661 ggagcactat ttcgacaaca ttaaaacgat tcaaggcgga gtgcaggcag ccgaaatcat
      721 aatgctcatc accatcatct acacgctgat ttttggagtt gcatattatc ttctgaccaa
      781 ctggatgctg aaaaacaagt taaatctaga ataagcttta cgcaaataca gcttgagggc
      841 gcgtcacagg acgcgccttt tgtattttta gttgaaaaaa gcatggcgaa caactataat
      901 gttctcataa ttcccggaat cggccggttg caatagcata agtatttttg aaataaatac
      961 ttcacatatt ataaccatac gagttcggag cattttgact gcagcccgag agctgctttg
     1021 aaacgaatca atagtgagga gtgatttccg atgaatgcaa tcagtgccaa aggccttacc
     1081 aagtcctatg acggaaaggc tctggctttg gacagcctga atcttgaggt tccgcgcggc
     1141 ggtattttcg gtttcctggg gccgaacgga gcggggaaaa cgaccaccgt taagcttctt
     1201 accggacttt tgaaacctac aaagggagaa tgcagggtgt ttgaccttga tccgggcaga
     1261 aagccgtgtg acgtacaccg gatttgcggc gtcatgacgg aaagcgccaa aatgtacggc
     1321 cagcttaccg gaatggaaaa cctgatcttt tttgctcaga cctccggcat gacgcaggag
     1381 gcctgcggtc tgaaagcggg tgaactgctc aaacagcttg acctgtggga tgcgcgcgat
     1441 aaaaagctga gcgcgtattc caccggcatg gcacagcgcc tttcccttgc ccgggcgctg
     1501 atcaattccc cgcaggtgct ttttctggac gagcccacca gcgggctgga cccggaatcc
     1561 gcgcaggcgg tcaacgagct gattatgact ctggcaaaga attcgggaac cacggtgttc
     1621 ctatgtacgc atcagcttcg ttacgcagag gacctttgca gcagctacgg aattatcgag
     1681 aagggaaagc tccttgcttc cggcgatctg cagtccctgt gcgatgcgat cggctgccgc
     1741 atccgagccg gcttccggct tgccccgggg gaaacgctgg agggctttgc cgaagaattc
     1801 ggctggtggc agactgagat tgcaaaggaa gaggaaatgc ccgatctgct gaagaaaatt
     1861 atggacgatg gccgccatgt ttacgaagca cgactgatcc gtccgactct ggaagatgta
     1921 tatttcagct ataccgaacg tcaggaggcg cagcaatgaa attgttttca acggctgaaa
     1981 aagcaatcat gaaaaaggat tttggcgaaa tctgggatac caagatggca cgcaataccc
     2041 tgctcctgct cccgataatt ctggtggtga ttctgcctgc cttttacctt gccttgattt
     2101 attttatccc cgcggagcag atcaacggcg cggataaaat gttgaagctc cttccgccgg
     2161 aagcacaggg cttttcggta cagcagtcga tgttttatat gatgacgaat tttctgtgcc
     2221 cgatgttctt tttgatgatt ccgctcatgg tttcaagcat atccgcagcg tgcagctttg
     2281 tcggcgaaaa ggagcgcagc acgattgaga cgcttctcct taccccgatg agcatgagac
     2341 aaatctttaa ggcgaaggtt tttggctgcg ttattttatc cggcatttcc acgctgattg
     2401 cctttgtcgc gttttcggtc gtaacctctc tcggagatct tattatgaaa atgccgtttt
     2461 tcctgaactg gaactggctg gtgctcctgc tgttctttgc cccggcaatc actgttttcg
     2521 gcgtgatctt tatggtgatt ttttccggca aaagcaagag ctatatggaa tccttccaga
     2581 cgtcaagtta tatcgtactg ccggttatcc tgctcttcat gggacagttc acgggtctct
     2641 ttcagctcaa cgcgctgatt ctgctgctga tttccgctgt tatcatcatt ttggatatcg
     2701 tccttctgct gttcggctcc cgttccttta cgccggaaaa actgttaaag taacggaaca
     2761 tttgccttaa ccgccaaatt gtattataat agatataata aaaggttaag acaggaaggt
     2821 gattgggtgg ctgcagattt gcattgtcac accaaaatgt cggatggttc ggtcagcatt
     2881 gatgaagtcg ttatgcttgc caaaaaaaga ggcttgtcta cgatcgcgat taccgatcac
     2941 gatacgtttg ccggtacaac tagggcaaaa atctttggcg acaggcatgg aatagaagta
     3001 atatccggcg tggaattttc aacgatggac cccgcaacag gccgcaaggc gcacatttta
     3061 tgttacctgt gcgacaaccc ggatcgtctg gtcggtctgt gcaagcgtac cgcagacagc
     3121 cggcggcgcg ccgcaagcat catgttacag aagacgatgc gaatttaccc gatcacggcg
     3181 gatatggttg tccgccgtgc acacggaagc acgaatatct tcaagcagca tatcatgcac
     3241 gcactgattg acgcaggata cgcaagccac tttttcggag ccactttcaa aaagctgttc
     3301 aattcaaaaa acggtttggc ttactgcagt gttgaatacc cggacgttca cgacgtaatc
     3361 aaacaaatac acgatgcggg cgggattgcg gttcttgccc acccggggga atacaacagc
     3421 tacgagctgc ttgatcaact ggggtcaagt cacgaaatcg agggcgttga ggtctggcac
     3481 ccgcgcaaca aaccggggga tgaggaaaaa ttcattgccg cggcgaaaaa gtacagtctg
     3541 gttatgacgg gcggcacgga tttccacgga atgtacacaa gcgtgcacac gccgatcggt
     3601 acctgtacaa cgccggatga ccagctggag gcacttaaaa aacgcaaggc acaaatgaca
     3661 gaataggtga ggaaatcagt ggaataccat tacaaaacaa aagggacctg ctcttctgag
     3721 ataatggtgg agttggacgg ggacacaaaa aaagctgtag aaatagtcgg aggatgcaac
     3781 ggaaattcaa aaggaatttg ttcgttggtt cagggaatga aggcggatga ggtaattcgg
     3841 cgctgtaagg gaatccggtg cggattcaag ccgacctcct gtccggatca gctcgcaaac
     3901 gccttgttac atgctcagaa gcagcagagc ggaaaataga taaagggctg cgaagaatcg
     3961 cagccctttt ttgtgcggtt agcagcagcc gttgttgccg catccacgat cgcagtcgtt
     4021 tccaccgcag cagcatacca ggatgatgat aagaaggata acccaagtgc aggaattgcc
     4081 gccaccaaag aaattgttac acataattgt ttgcctcctt tcggtttaca atatataata
     4141 ttgcataggc ataaaacgtg ttacacataa aattggctct ttaaaataaa ttttatatta
     4201 gaatttatat tgttttcaaa aacggtagcc ctctttattc tatgcggcaa tgccgaattt
     4261 ggactgagat ttcgggactc tatggaggaa taaaaatgtt atgtgaatgc tgtgggaaaa
     4321 acccggttac cacatatgtt aaaacgatca ttaacggtga acttacaaaa tattcgcttt
     4381 gcgcggaatg tgcgcaaaag ctcggctacg gcagccttct taccgggctt ggcctgaatt
     4441 acgggagcct tctgggcgcg tttttcgggg aaccggagca ggaagacaag gactctgtgc
     4501 gctgcaaatg ctgcggctgc agctttgaag atattgcccg cagcggcagg gtgggctgcg
     4561 cggaatgcta ccataccttc tataaccgcc tcattcctct tattcagcgt attcacggaa
     4621 acaccaaaca ccgcggcaag gttccgggcg gagagcagct tcagcctagg ccccagagcc
     4681 agctgagcgt gatgcgccgg gagctgcgcg aggcaatcaa ttccgaaaat tttgagcatg
     4741 ccgcgaattt gcgcgaccga atccgggaat tggaggggaa agaaaaccgt gagtaaatgg
     4801 tatgaaaaaa gcggcgcgga aggtgacgtg gtgatcggta cgcgcatccg tcttgtccgg
     4861 aatctgaacg gttttccttt tcctgcgcgt atgagcaaag ccgaccgccg catcctcttg
     4921 caaagagtgg cggaaacagt tggcagctgc gcgcaggcat acggttttca gttccttaat
     4981 atggaggaaa cctccaaaac gcaggcggtt tcccttgtag aacggtatct tgcaagcccg
     5041 gaatttattt ccgaacccga tggcagagga atgctgctga cggaggatga atccctcagt
     5101 atcatgctca acggagaaaa ccatctgacc atacagacca ttacggaagg gcttgatctg
     5161 cagcaggctt atcagatggc ggatcagctt gatacgattc tggataggtc tctgcatttt
     5221 gcctttgacg aggctctggg ctatctcacg cagaaccccg ttgatctcgg caccggcatg
     5281 cgcgcttcgc tggtccttca cctgcccgcg atgcaggacg ggggaggaat cacaaggatt
     5341 tccggcagtc tgtccaagct ggggctgtcc ttgcggggaa tctacggttc cggcaccact
     5401 tcaaaaggag ccgtttatga actgtccaat caggtaacac tcggtctttc cgagcaggaa
     5461 gcgcttgcaa acctccaaag tattgcgctg cagatcatcc ggcaggaacg cacggcccgt
     5521 acggagcttg gtgaaaggct ggacgttcag gatgccgtaa gccgctctct gggaattttg
     5581 cagagtgcaa agcttatgac aaacgaagaa tttatggggc ttatttccaa cgtccgattc
     5641 ggtgtagcac agggactttt tgataaaata agctacagta ccattaacaa gctggttatt
     5701 gtcgcacagc cggccacgct tgcactgtac agcgggaaaa agctgactgc gaacgaacgc
     5761 catcttctgc gggcccaaac ggttcaaaat ttattaaagg cggatgattt ttatggagca
     5821 gccaattccg agcaatgaag agctggcggc ggcgctgctt tgggcgctgg gagaaaatat
     5881 accgcatgac cgggcgcttg aagcggaagc cattcggctg aaggctgaga aagcaagcag
     5941 cgaggaactt ctgcaaaaag tgattgcact gtgctcaaat tcaaagacgc ccatgcagct
     6001 gtatcttacc gcaaaagcat acagcctgct tggcagcggg ttttacagtc agaccatcga
     6061 atcaatgcag gaatatctga aaaccggcgg atgggataag ctttccggcc attcggtgat
     6121 ggaaaacgga atcctaatca accatctgtg tatgcagcgt gctgcggacc tggtcgtgct
     6181 tgcaagggca caggagagct ccggcaaatt ggaagatgcg ctggagagtt ttacggaagc
     6241 ctatcgtttt gaaccgtatc gggcgatgca cgcggttaag gtcgccgatc tgctcgtaaa
     6301 gctgcacggc agggaagagg cgcttttgtt tcttcgccat caaaagcaaa gccgctacta
     6361 cagtcctata aaatataccg atacgctcgg agaagtgcgt tacaacgacg agttcaggcg
     6421 ccttttggac gcgcatattc tcaagattca aaatatagga taaatggata tttaaattgc
     6481 aaattgccga agaatactta tattccccgg caattttttc tattatttat gtatgtgact
     6541 ctcatccatg ccaataagaa aaattaatat ttaacgtctg cgaggacggt ctgctgagac
     6601 tgcggtatat ccggccggaa actttttacg ttgtcggctt tttcatctgc ttttgtttcg
     6661 ctaatctgaa cagatttgtc ctttagttta aactgcttca ccagagtttt caatgccttg
     6721 gcctgcccgg agagctcctc actggccgcg gcgctttcct ctgcggttgc agaattggtc
     6781 tgtacgacgc tggagatttg ctctactcct gtagtgacct ggctgatttc ttccgactga
     6841 tggtttgttg cttcggaaat ctggttaatt gtatcggtta ccgtttttat gttgtcaacc
     6901 acacgtacca aggattgggc cgtttcaccc gcgatctttt ggccgttcgc aacctgcttg
     6961 atcgagtttt caattaaaac ggttgtgtcc tttgccgcca gcgcactttt gctggcaaga
     7021 ttgcgaacct catccgcaac aaccgcaaag ccttttcccg cggcgcctgc tcttgccgct
     7081 tccacggccg cgttcagcgc gagaatattt gtctggaatg cgatgtcctc gatcgttttt
     7141 ataattttac cgatttcatt ggatgattca ctgatttggg acatggagcc aagcatttca
     7201 tgcatatggt tgttgctgat ttcaacttcg gagctgacat gatttacatt tctgcttgct
     7261 tcggcggcat gttccgcatt ttccctgacc tgggaggaaa tctcaacgat cgtggcggaa
     7321 agttcctcca cggagcttgc ctgctcggct gcgccctgtg ccagtgcctg cgcgccaccg
     7381 gaaacctgct cggagccgtt ggaaacctgt tcggaagctt cgttaatttc agacagcgtt
     7441 tcgcttaagg atgaaataat cccatggtag gctatgctca agcctttata atcgccgaca
     7501 tattgcatct tagagggcat ggccaggttt ccgtgcgcta ccttatcaag atgttccgtc
     7561 aggtcggcta tatatgctct gattgaagtt gtggattctt taaatgccgc agccaactgc
     7621 ccgatttcat tctgagaatc ataatttacc tgtacattta aatcgccctc tgccattctc
     7681 tgtgcagctt ccgccatttc ttccacaggc ttgctgatac tgcgtgcaat ggacagggca
     7741 atcacaaggg aaaggataag ggaagcacac agaatcgcaa ggatgatcat acctgaggcc
     7801 gacgcctgag cagaaaggct ggcagaaagc gaccccagct ttcttatttc aaaaaagccg
     7861 acgatactgc cgacaccgga aagcacagct accaaaataa acgcaaatac cagtttgttt
     7921 ccaattttca tattttttat catgactatt cccttcttta aatgaataaa gataataaga
     7981 ctcagcttaa gatataccag ttgtatcgaa tcaaacagtc cccgtctgtt ccattcgtac
     8041 tcagacagcc aagtctattg gatccattgt agcagattta ccttttctat aacgcaatga
     8101 atgattaaat aatattacga tagtgtatat atgttttttg cggttttcag agtcaagtaa
     8161 aagcattcac tttttctcag ccgcttggat catatcatat tgcatcacat cctagtcctg
     8221 caatgctttc caaatttgga aacatatcgc agttaacata ttgtttatat aatactcgga
     8281 aatacaacaa aactcaatag catacattta tataaattat gcaaattaaa tatatattat
     8341 ttcgataatg taacattctg ttacttcatt aaaggagaga tttttttagg gaagattccc
     8401 ggataggcag acaaacagaa agggctgttt cggaggctcg gctccggaac aaccctttct
     8461 gcgtaaaaca cgaaacgaat gctctgtcag gtaaagtcag tggaaatttc cttttcgctg
     8521 cccaggggag aatacacgaa ccgcttcatt ttgttcccat caaaaaagta ttttggaggg
     8581 ctggaacggt aagagtaatc aaaggt
//
LOCUS       DATALY010000272        10921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_746345,
            whole genome shotgun sequence.
ACCESSION   DATALY010000272 DATALY010000000
VERSION     DATALY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746345"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1212
                     /locus_tag="VHR42_07490"
     CDS             196..1212
                     /locus_tag="VHR42_07490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEX3027054.1"
                     /translation="MIINFNVNPYTTKETSVGTVDYGNVHQFPFIVIDLDSFIVSCEI
                     QSGINFDNEQISHNLQIGKFCSLADKIKFMIAVNHDYESVTTGACSFLNGVVRPFRLK
                     QKNQIIIQNDVWIGSGATIMSGVTIHNGAVVAAGSHVVKDVPPYAIVGGNPAKIIKYR
                     FNEDQIEKLLKISWWNWDIKKISDNKMFFLKNIDEFINAFYEESLREEILTLNYKKEK
                     PILLFFPDINENYPITEHVIKNYCEHYGDSGEAVLFMYLQSNNNVDEITDKINLILKK
                     CRCDKNDNIVLNIDNLPNEKPLFKISECYITTRAKETVLRTCYADEFGVKILSGVDKP
                     VFGD"
     gene            1695..2084
                     /locus_tag="VHR42_07495"
     CDS             1695..2084
                     /locus_tag="VHR42_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027055.1"
                     /translation="MSNYASRNQFNVQGQYGGPYPSGQVQNPMPQQPAGSVTQQGAQM
                     PAMPRMPAAQSFAVTDESMQYLNGLLRTQIGKKITVTFLIGTNTQVDKTGTLLEVGVN
                     YIVINEIETDDLVVCDFYTIKFVQVYY"
     gene            2202..3752
                     /locus_tag="VHR42_07500"
     CDS             2202..3752
                     /locus_tag="VHR42_07500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3027056.1"
                     /translation="MNVISLLSMAAALVYIHLGIIIIKIDRKSKLNRMFFCFCLAMTI
                     WSFGYAFVYISTNSLTTQLWVKLSALGWCSYPAFLLATILYFTENHLVHSKKGMLLLF
                     VPAVLFFALDVIFFWPEFTTPAIVVRFFYQWNFIYNTSYLAACVVLLIRSIRRSKKKM
                     LLYQSRIVLVSIILPNVILYLMNAAEHFAGWTPVECGQLYPMVSIVALTYAIRRYGLF
                     HYRKVMTDEIMSELMDLVIFLDSDHKIIKINKHAQRILEYSSEELIGKTADAVFPNQG
                     IDELISLSRDKSLVVHYDELQCVKKGGGMIPVMAKLFLTVDPRLRQTTFVVLIGQDIS
                     QKKRLEIEIANHKETQKYITYLAYHDSLTGLPNRKRFVEKLCDMLETAEQNEEIFAVY
                     YLDLDDFKQINDENGHEAGDELLRAAGKLISQNIGEKNLAARIGGDEFVLLITGLKTA
                     GEAEKFAKKIRTMFEYSVDWKGKTLPIRISLGWSVYPNDAVKAQDLIHCADMRMYECK
                     RDRKKNRV"
     gene            complement(3796..4440)
                     /locus_tag="VHR42_07505"
     CDS             complement(3796..4440)
                     /locus_tag="VHR42_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX3027057.1"
                     /translation="MTLLNQISENLQKGRVKAVQQLVSQAIDEKTSAKEILESGLLVG
                     MNTVGVLFKNGKIYVPEVMLAARAMNGAMAILKPLLVGEGVESLGRVCIGTVKGDQHD
                     IGKNLVKMMMEGSGLEVIDLGVNVAPEKFLETAMEKKCRIVACSALLTTTMARMKDVV
                     ELFRSKGIRDQYKIMIGGAPVTEQFCRQIGADAYTPDAASAAEVAADFCRASNS"
     gene            complement(4492..4863)
                     /locus_tag="VHR42_07510"
     CDS             complement(4492..4863)
                     /locus_tag="VHR42_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027058.1"
                     /translation="MKVAVVGSRNIYPDTFNRILSGIPAGCSEIISGGANGVDLLAKK
                     AAKNLNIRYVCVRPNYRKFGRLAPLIRNSEIVDRSDCVLAFWDGVSRGTRHAIACCIK
                     RRKPVKIILINTHNKIYQNYS"
     gene            5147..6382
                     /gene="spoIVB"
                     /locus_tag="VHR42_07515"
     CDS             5147..6382
                     /gene="spoIVB"
                     /locus_tag="VHR42_07515"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HEX3027059.1"
                     /translation="MRKQIKIIAAVCGIVMLSLLTAGLLLADRIPDRFSVIEGQELTF
                     ENRYPIVAVSANQPQSMEVSGTLKAGEQYKTDLKLFGVVEVKNVSVKVVKEARVVPCG
                     YAFGVKLHTEGVVVVGLSDVDTKDGSENPSYAAGIRMGDIILAINGKTVNSNKEVSEI
                     FNQSDGKPMKITLRRGNVGFEVKMTPVKSVSQNQYKTGMWVRDSTAGIGTITFTVPST
                     GLFAGLGHGICDVDTGEILPLMTADIVKVDISGIARGNKGQPGELEGKLHDETWGSLY
                     SNTQTGVFGILNNSSASKAIPVAMRQQVHEGPAKILATLDKSGPKEYSISIEKVHFND
                     AAPTKNMIVRITDSSLIAKAGGIVQGMSGCPIIQDGKLVGAITHVFVNDPLRGYGIFA
                     ENMIDTAGQINNSFLKQAS"
     gene            6676..7470
                     /gene="spo0A"
                     /locus_tag="VHR42_07520"
     CDS             6676..7470
                     /gene="spo0A"
                     /locus_tag="VHR42_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HEX3027060.1"
                     /translation="MLDNKLKVLVADNSIEMGQVCAATLKSYGMSVTTISKDGQEILD
                     QINSEMPDVILMNAFMTRIDAIGVLNAIKNMNLPKKPLMIVMSTDNTGAISSQVMDAG
                     ASYFFPMPFDHDLLAERINQLVGIGNDRRSTLIKPDIQYNSTQDIEIIVTKIFHQIGV
                     PAHIKGYHYLREAIMMAIKDIDIINSVTKQLYPSVAKKYETTSSRVERAIRHAIEVAW
                     DRGDVDILNSYFGYTIHNSRGKPTNSEFIAMIADKLRLQLHINSAS"
     gene            complement(7536..7970)
                     /locus_tag="VHR42_07525"
     CDS             complement(7536..7970)
                     /locus_tag="VHR42_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3027061.1"
                     /translation="MNIKVMYYSKGGNTKKVAQTISQAINQPAESIPPAYPAENIRLL
                     FLGGGVYGGKLDPKLTEYINTLTPEKIKNVALFSTSGSGQGDGIRLMKEALEKKGLHV
                     LDENFVCCGKYFIFFKRNHPDADELKAAQDFAKKAVEKLEKE"
     gene            8209..9735
                     /locus_tag="VHR42_07530"
     CDS             8209..9735
                     /locus_tag="VHR42_07530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX3027062.1"
                     /translation="MTKYILAHDFGTSANKATLYTTDGDLLESNTYSYETNFFSGNCA
                     EQNPEDWWKAICGSTRAIMKKINPRDVLAVSFSGQTHGCLAIDREGRALRNSIIYCDN
                     RSASQMNEIGKKIDEFEFYKITGSRFSAVLPVAKLMWIRDHEPDTYKRTYKAVNAKDY
                     IVSRFTGRIVTDVNEASYTGACDLKTGKWSEKIINISGIDPDKLPEIKPSTFIAGEIS
                     AKTAEETGLAAGTPVVIGSSDGSCATIGSGAVYENSSYLCLGSSAWICLTSSKLDFDP
                     KMDNSMGVGAIPGKYHHYGTMQSACTVYNWLRSQLCAKEEMEAERLKSSVYSLMDQLA
                     ESSLPGARGVLFHPYPMGERSPRWNSAATAAFLGIDINTRREDMIRAVLEGVAYNLKC
                     IYESLNKLVPMGEISIIGGGAKSDVWCQILSDVLNCRITRPTRLDQATSMGAAVIGGV
                     GAGVFGGFEAVEKFIKISKVYRPNPEARERYDEMYSLFNSSYEALLGFYQQRSLLKQS
                     "
     gene            9755..10894
                     /gene="nagA"
                     /locus_tag="VHR42_07535"
     CDS             9755..10894
                     /gene="nagA"
                     /locus_tag="VHR42_07535"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX3027063.1"
                     /translation="MAVIKNAKIFDGEKMLGQTAVVFENGKVTGFLDSSDCADAIDAG
                     GKLVTPGLVDIHIHGSFGIDFMEKGALETVCKKLPQYGVTSLLATSVTESDRAIRDFL
                     GYTRKAMKMEKGTRILGAHLEGPYFSYENRGAHDENELRDPLPENYHAMTDGFEDIIT
                     RVSIAPEKKGGFELVKELVEDGVLVSVAHTNANADEMEKAIDLGLSLCTHTFNGMFPF
                     HHRNTGSIGVVLTDDRITCEFIPDLHHITKLAIRLILKSKGTENTIICTDAIAGALVE
                     DGDYVLGGLAVVVKDSVAHLKSDGRLAGSTISLDWGVRNLVNEVGVPVETALRMATRN
                     PAKAIGRDHQVGRLAVGSNADILVLNDDLTVERTFIRGVEEYHRV"
BASE COUNT         3068 a         2255 c         2629 g         2969 t
ORIGIN      
        1 tgaaactcaa ttgccaaaaa gggtagaatg ttttgaactg gtgagatagg gcttatgttt
       61 ttttgacctg attaagaatc gcttggttaa tttattcttt ctattaaatc ttggtcatta
      121 cgaatattga acgcgttgca ataaaatagg ctgtcgctgt ttcaaagcct caccatcaat
      181 agaaaaggaa aatttatgat aataaatttt aatgtaaatc catatacaac aaaagaaacc
      241 agtgttggta ctgtagatta tggcaatgtg catcagttcc cgtttattgt tattgatctt
      301 gacagtttca tagtttcatg tgagattcaa agcggcatta attttgataa cgaacaaata
      361 tcgcacaatt tacagatagg taaattttgt tctcttgcag acaaaattaa gtttatgatt
      421 gcggttaatc atgattatga aagcgtaaca acaggagcat gtagtttttt aaatggtgta
      481 gtaaggcctt tccggcttaa gcaaaaaaac caaatcatta ttcagaatga tgtttggata
      541 ggcagcgggg caaccattat gagtggggtc actattcata atggagctgt tgtcgcggcg
      601 ggttcacatg tcgtaaaaga cgtgccccca tatgcgatag tgggaggtaa ccccgcaaaa
      661 ataatcaaat accgttttaa tgaggaccaa attgaaaagt tattaaaaat atcttggtgg
      721 aactgggata tcaaaaaaat ttcagacaac aaaatgtttt ttttaaaaaa catcgatgaa
      781 tttatcaacg cattctatga ggagtctttg cgtgaggaaa ttttgacttt aaattataag
      841 aaagaaaagc ctatcctcct gttttttcct gacattaacg aaaattaccc gattacagag
      901 catgtcatta aaaactattg tgaacactat ggtgatagtg gagaagcagt gttgtttatg
      961 tatttacagt ctaataataa tgttgatgaa attacagata aaatcaatct tatattaaaa
     1021 aaatgcagat gtgacaaaaa cgataatata gtattaaata ttgataattt accgaatgaa
     1081 aaacctctgt ttaaaatctc cgaatgctat attacaacac gtgctaaaga aacggtttta
     1141 agaacatgct atgcagatga atttggggta aaaattcttt ccggtgttga taagccggtg
     1201 tttggcgatt aggacaaacc tttcgatcat gcgcaatgcg aatgtctgag cactgtgtta
     1261 tataaaatag ttgtttggac taattcaaag aaaataataa ggtaaaaagc ccttactcca
     1321 gaaccgtccg ccaaacgatc tccagaagtc ggacacaatt cattacgtct ccgtaaaagc
     1381 gggggcgttt tgtttttgct gaaaacggga tgtgctgtca aatgcaggct aatccttgaa
     1441 accggcgtct gtgccacgaa tgttcacaag ctgcggcggc ggattcaggg atatgcgctg
     1501 cagaaaagaa tgtcaatcgt ttattcgccg catatagtgt taatgatatt gagtttgaaa
     1561 gcttcgcccg ccggactgcc gttgcagaat atcaattcat gagtctgccg gtatgtctga
     1621 tttgggcgga aacccgttgc agagattcag tcgctgcgaa ttcaaatcag ctaccggtaa
     1681 aaggagaaaa gtttatgagt aattatgcct cccgtaatca gtttaatgtc cagggtcagt
     1741 atggcggtcc atatccatca ggccaggtcc agaatcctat gccacagcag ccggccggca
     1801 gcgtgaccca gcagggtgcg cagatgccgg cgatgcccag aatgcctgcg gcgcagtcgt
     1861 tcgctgtgac agatgaaagc atgcagtact taaacggact cttaagaacg cagatcggta
     1921 aaaagataac cgttacgttc ctgatcggaa ccaatacgca ggtcgacaag accggaacgc
     1981 ttctggaagt cggagtaaac tacatagtaa ttaatgaaat tgagacagat gatcttgtgg
     2041 tctgtgattt ctacacaatc aaatttgtgc aggtttatta ctgacatcag aaaaggcccc
     2101 cgaaacgggg ctttttcgat gcgtgcgatc tttgcagtcg ccttgataat gttggcagga
     2161 gtggtataat agaaattatc cctttttaag aggtgatttt tatgaatgtt atctccctgc
     2221 tgtctatggc ggctgccctt gtttatatac atctcggtat cattattatt aagatcgaca
     2281 gaaaatcaaa gcttaacagg atgttctttt gcttttgcct tgcgatgacg atctggtctt
     2341 tcggttatgc gttcgtctat atttctacga attcgttaac aacccaactc tgggtcaagc
     2401 tatccgcttt ggggtggtgc tcatatccgg ccttcctttt ggcgacaatc ctatatttta
     2461 ccgaaaatca tcttgtgcac agtaaaaagg gcatgctgct gctttttgtg ccagctgttc
     2521 ttttttttgc gctggacgtc atttttttct ggccggaatt tacgactccg gcgattgtgg
     2581 ttcgcttttt ttatcagtgg aactttatct acaacacttc ctatttggcg gcctgtgtcg
     2641 ttttgctgat cagatccatt cgccgaagca agaaaaaaat gcttttatac cagagcagaa
     2701 ttgtgctggt ttctatcatt ctgcccaatg tgatcctata tttgatgaat gcggcggagc
     2761 atttcgccgg ttggacgcct gttgaatgcg gccagcttta tcccatggtt tctattgtgg
     2821 ccttgaccta tgccatccgg agatatggat tgtttcatta ccggaaagtc atgacggatg
     2881 aaatcatgtc agagctgatg gatctggtta tttttttaga cagcgaccat aaaatcataa
     2941 aaatcaataa acacgcgcag cgaatactcg aatattcttc ggaagagctg atcggaaaaa
     3001 ccgccgacgc cgttttcccg aatcagggaa tcgacgagct gatttcctta agtcgggaca
     3061 aatccctcgt cgttcattat gatgagctgc aatgcgttaa gaaaggcggg ggaatgatcc
     3121 cggttatggc gaagctgttt ctgaccgtag acccgaggct tcgtcagaca acgttcgttg
     3181 tgctgattgg gcaggatatt tcgcagaaaa aaaggctgga aattgaaata gccaatcata
     3241 aggaaacgca gaagtatatt acatatcttg cctaccacga ttcgcttacc gggcttccga
     3301 atcggaaacg ttttgtggaa aagctctgtg acatgctgga aactgccgag cagaacgagg
     3361 aaatattcgc cgtgtattat cttgaccttg atgattttaa acaaatcaac gacgaaaacg
     3421 ggcatgaagc cggcgatgag ctgctccgcg ctgccggtaa gctgatttca cagaatatcg
     3481 gagagaagaa tctggccgca agaatcgggg gcgacgaatt cgttttactg attaccggct
     3541 taaaaacagc cggggaagcg gagaaatttg cgaaaaaaat ccgaactatg tttgagtatt
     3601 ccgtcgactg gaaggggaaa acgctgccga tcagaatcag cctgggttgg agcgtttacc
     3661 cgaatgacgc cgtcaaagcg caggatctta tccattgtgc cgatatgaga atgtatgaat
     3721 gcaaaagaga tcgaaaaaaa aacagggttt aaacagcttg gctgtttaaa ccctgtttta
     3781 tatcggagaa gcgatttatg aattggatgc cctgcaaaaa tcagcggcga cttccgccgc
     3841 cgaggccgcg tcgggggtat aggcatcggc tccgatctgc ctgcaaaact gctcggttac
     3901 cggggcgccg cctatcatga ttttgtattg atcccggatt ccctttgacc ggaagagttc
     3961 aacgacatct ttcattctgg ccatggttgt ggtcagaagt gccgagcatg cgacgatccg
     4021 gcactttttt tccatcgccg tttccagaaa tttttcggga gcgacattaa cacccagatc
     4081 aatgacttcc agcccgctgc cttccatcat cattttaaca agatttttcc cgatgtcgtg
     4141 ctggtcgccc ttaacggttc cgatgcagac tctgccgagt gactcgacgc cttcgccgac
     4201 aagcaggggt ttcagtattg ccatcgcacc gttcatcgcg cgggcggcaa gcataacctc
     4261 gggaacataa attttgccgt ttttaaacag taccccaacg gtattcatcc cgaccaaaag
     4321 gccggattca agaatttctt ttgcggaggt cttttcatca atagcctgtg aaacaagctg
     4381 ctgtactgct ttgacccgtc ctttttgaag attttccgat atctggttta gaagcgtcaa
     4441 tttgaatccc cccggttatc gcaaataaag tttcgccttt ctttgcatgt attatgaata
     4501 gttttgataa attttgttgt gagtgttgat taaaataatt tttaccggtt ttctgcgttt
     4561 gatgcagcag gcaatggcgt gtctggttcc tctggaaacc ccgtcccaga atgcgagaac
     4621 gcagtcggag cggtcgacga tttcgctgtt gcggatcaaa ggtgccagcc tgccgaattt
     4681 tcggtaattg ggccgaacgc aaacatatcg gatattcagg tttttcgcgg cttttttcgc
     4741 aagcaaatcg acaccgttgg ccccgccgct tataatttcg gaacatcccg ccggaatacc
     4801 ggatagaatc ctgttaaagg tatcggggta aatatttctg gaaccgacta ccgctacttt
     4861 catgtcatca ccgccctgaa aaaatttgca gcctgttttt tccgtcacag gcagcagaat
     4921 tatatataga gtaacatttt aaagaaaaaa tgtaaataaa attaccgccg aattttactt
     4981 gccgttccaa gaaaattcgg tttatttcaa tacatgcatg tcaattatta atcatttgct
     5041 gttttgtttt ccattcggcg cattttttac atttcatatc acgggtctga gaaggaaaat
     5101 taataaaggt catttgtaaa aaaaagacgt ccgacggagg aacagaatga gaaaacaaat
     5161 aaaaataatt gccgccgtat gcggcattgt aatgctatcg ttgctgacgg ccgggttact
     5221 gctggccgac cgtattccag accgcttttc cgtaattgaa ggtcaggagc ttacatttga
     5281 aaacagatac ccgatcgtgg ctgtttctgc caatcaaccg caatccatgg aagtatcagg
     5341 cacattgaag gcgggagaac agtataaaac ggatctgaag ctttttggcg ttgtcgaggt
     5401 aaaaaatgtt tcggtaaagg tggtaaagga agcccgagtc gtcccctgcg gctatgcatt
     5461 tggcgttaaa cttcataccg agggtgtggt tgttgtcgga ctttccgacg tcgatacaaa
     5521 agatgggtct gaaaatccgt cttatgcagc aggaatacgc atgggagaca tcattctggc
     5581 aattaacggg aaaactgtaa acagcaacaa ggaagtgtcg gaaattttta atcaaagcga
     5641 cggtaaaccg atgaaaatta ctttaagaag agggaatgtg ggctttgagg ttaaaatgac
     5701 tccggttaaa tccgtcagcc aaaatcaata taagacagga atgtgggtca gagacagcac
     5761 ggcaggcatt ggtacgatta cctttacggt tccttccacc gggctgtttg cgggccttgg
     5821 gcacggaatc tgcgatgttg atacggggga aatccttccg ctgatgactg cggatattgt
     5881 aaaagtcgat atttcgggaa tcgcccgggg aaataaaggg cagccgggtg agctggaggg
     5941 caaacttcac gatgaaacat ggggcagcct ttacagcaac acgcagaccg gagttttcgg
     6001 aatattaaat aacagctctg ccagcaaggc gattccggtt gcaatgagac agcaggttca
     6061 tgagggccct gccaaaattc tggcgacgct cgataaatcc ggcccaaagg aatacagtat
     6121 ttcgattgaa aaagtgcatt ttaacgacgc ggctccaacc aaaaatatga ttgtccgaat
     6181 aaccgacagc agtctgattg ccaaagcggg cggaatcgtt cagggaatga gcgggtgccc
     6241 gattattcaa gatggaaaat tggtgggtgc aattacccat gtgtttgtga atgatccgct
     6301 caggggctac ggcatttttg cggaaaacat gatcgacacc gcaggtcaaa taaataactc
     6361 atttttaaaa caggcttcat aagtcctgtt gaaattgctt ttgaattatg tgaatattca
     6421 acaagaaaca ccgcccaatg ttgatgagtt cctaaaattt tcaaaaatat cttgaaaatg
     6481 tatatattta gagtaaaata aaaattgtaa ttaattttta attaattatg atagatttta
     6541 ggcgtcaata ttcaaattat ccatgtccag cttccataaa aaacagaaaa aaatagaaaa
     6601 aattggaaat ctatattgca atatttacca atatatggta aattatattt gcaaacaaaa
     6661 atattggagg ttttaatgtt ggataataaa ttgaaagtgc tcgtcgcgga taatagtatt
     6721 gagatgggac aggtgtgcgc agcgaccctc aaatcttatg gcatgagcgt tacgacgatt
     6781 tcaaaagatg gtcaggaaat tctggatcaa ataaatagtg aaatgcccga cgtaatcctc
     6841 atgaacgcgt ttatgacgag gattgatgca atcggggtgc tcaatgccat caaaaacatg
     6901 aatcttccaa aaaagccgct catgattgtc atgtcgactg acaacaccgg agcgatcagc
     6961 tctcaggtga tggatgcggg ggcctcctat ttcttcccga tgccgtttga tcatgatttg
     7021 ctggctgaaa ggattaatca gcttgtagga atcggaaacg accgccgctc cacgctgatt
     7081 aaaccggata tccagtataa ctcaactcag gatatcgaaa tcattgtaac aaaaattttt
     7141 catcagatcg gtgtgcccgc gcatatcaaa gggtatcatt atctcaggga agcgatcatg
     7201 atggccatca aagacattga tattattaat tccgtaacca aacagcttta tccatcggtt
     7261 gcgaaaaagt atgagacgac gtcttccaga gtggaaagag cgatcaggca tgccattgag
     7321 gttgcctggg acaggggcga cgtcgacatt ctcaattcct atttcggcta tacgattcac
     7381 aacagcaggg gaaagccgac caattccgag tttatcgcga tgatagccga taaacttcgt
     7441 ttacaacttc atattaattc ggccagttaa cttaaaccaa aaaaattcct ccggctgcga
     7501 atagcgcagt ccggaggaat ttttctcata tcagattatt ctttttctaa tttttctacc
     7561 gcttttttgg caaaatcctg agccgccttt aattcgtcgg catcgggatg attgcgttta
     7621 aagaaaataa agtatttacc gcagcaaaca aaattttcat caagaacgtg aagccctttt
     7681 ttctccagag cttccttcat cagccggatt ccatcacctt gtccgcttcc gctggtgctg
     7741 aataatgcta cgttctttat tttctcaggg gtgagtgtgt ttatatattc ggtcagcttg
     7801 gggtcaagct tgccgccgta aacaccgccg cctaagaata acaatctgat gttttcagct
     7861 gggtaagccg gaggaattga ctcggccggc tgattaattg cctgactgat cgtctgggca
     7921 acttttttcg tgtttccgcc tttgctgtaa tacataacct ttatattcat tttgacgcat
     7981 cctttatata aattttgttt gactaattat atcatagtta tataatttaa acaaataaat
     8041 taatttacaa tcaaaccatt tgttgaatgg ggatgacaaa agatgtaatt acaagacttt
     8101 gcaggatggt ctccaaagaa caacggagag agattgaatg atttaccggg tcgaatttta
     8161 tacggaaaga tcaggcttta ataaaggaag ggcttagggt attgaaatat gacaaaatat
     8221 attcttgctc atgattttgg cacttcggca aacaaggcca ctctttatac gaccgatggg
     8281 gacttattgg aaagcaatac atattcttat gagacaaatt tttttagcgg caattgtgcg
     8341 gaacagaatc cggaggattg gtggaaagcg atatgcgggt ctacccgcgc aattatgaaa
     8401 aaaatcaatc cgcgtgatgt tcttgccgtt tcattcagcg gccagactca tggatgcctg
     8461 gcgattgacc gggaaggccg cgccctgaga aattcgatta tctactgtga taaccgatcg
     8521 gcgtctcaaa tgaatgaaat cggtaaaaaa atagatgaat ttgaatttta taaaattacc
     8581 ggttcgcgtt tcagcgcggt tcttcccgtt gcgaaactca tgtggattcg ggatcatgag
     8641 ccggatacct ataagcgaac ctataaggct gttaatgcaa aggattacat cgtctcacgg
     8701 ttcaccggcc ggattgttac ggacgtcaac gaggcgtcct ataccggagc gtgtgatctg
     8761 aaaaccggaa aatggtctga aaaaattatc aatataagcg ggattgaccc ggacaagctg
     8821 ccggagatca agccttctac ttttatcgca ggtgaaattt ccgcaaagac cgctgaagaa
     8881 accgggcttg cggccggtac accggttgta atcggaagca gcgacggaag ctgcgcgaca
     8941 atcggaagcg gcgcggtcta tgaaaatagc tcgtatttat gcctcggttc ctcggcgtgg
     9001 atttgcctga cgtcgtcaaa acttgatttt gacccgaaga tggataattc catgggcgtt
     9061 ggggcgattc cgggcaaata ccatcattac ggaaccatgc agtctgcctg caccgtttat
     9121 aactggcttc gctcccagct ttgcgcgaaa gaagagatgg aagccgaaag gctcaaatca
     9181 agcgtttatt ccctgatgga tcagctcgcg gaatcctctt tgcccggcgc ccgcggagta
     9241 ttgtttcatc cttatcccat gggcgagcgc tccccgcgct ggaattcggc cgcgacggcc
     9301 gcttttttgg gaattgacat caataccagg cgggaagaca tgattcgcgc tgttttggag
     9361 ggtgtcgcct ataatctgaa atgtatctat gaatcgctga ataagctggt tccgatgggt
     9421 gaaatttcga tcatcggcgg gggcgcgaag agtgacgtct ggtgtcagat cctttcggat
     9481 gttttaaatt gccggatcac aagaccgacg cggcttgacc aggcgacgtc tatgggcgcg
     9541 gcagtgatcg gcggagtggg cgccggagtg ttcggcggct tcgaagctgt tgagaagttt
     9601 attaaaatat cgaaagtcta tcggcccaat cctgaagccc gggagcgata cgatgaaatg
     9661 tattcgctgt ttaacagcag ctatgaggcg ctgctgggct tctatcaaca aagatcgctc
     9721 ttgaaacaat cataaaattt taggaggcat tctcatggcg gtaataaaga acgcgaaaat
     9781 atttgacggt gaaaaaatgc tcgggcaaac ggcggtcgtc tttgaaaacg gtaaagtaac
     9841 cggattcctg gattcgtccg actgcgcaga cgcgattgac gcaggaggaa aactggtgac
     9901 cccgggcctt gtggatattc atattcatgg ctccttcggt attgatttta tggaaaaagg
     9961 cgcgttggaa acggtctgta agaagctgcc ccaatatggg gtcacgtcgc tgctggcgac
    10021 ctcggtgacc gaatccgacc gggcgatccg ggattttctc ggctacaccc gtaaagccat
    10081 gaaaatggaa aaaggcacgc gaattctcgg ggcgcatttg gaaggcccgt atttttctta
    10141 tgaaaaccgg ggagcgcatg atgaaaatga gttgagagac cctctgccgg aaaactacca
    10201 tgcgatgacc gacggttttg aagacatcat tacgagagtc tcgatcgctc cggagaaaaa
    10261 aggcggtttt gagcttgtca aagagctggt ggaggacgga gttctggttt cggtggccca
    10321 tacaaatgcg aacgcggatg aaatggagaa ggcaattgat ctgggtctga gtctctgcac
    10381 acatacgttc aacggcatgt tcccgttcca tcacagaaac accggctcaa tcggggttgt
    10441 cctgaccgac gaccgtataa cctgcgaatt tatccccgat cttcatcata ttacgaaact
    10501 ggctatccgt ctgattttga aaagcaaggg aacggaaaat acgattatct gcaccgacgc
    10561 catcgcggga gccttggtgg aggacggcga ttatgtgctg ggcggtctgg cggtggtggt
    10621 taaggacagc gtggcccatt tgaaatcgga tggaaggctt gcgggcagca caatttcgct
    10681 ggattggggc gtgcgcaatc ttgtcaatga agtcggagta ccggtggaaa cggcgcttcg
    10741 catggcgaca cggaatcctg caaaagccat cgggcgcgac catcaggttg gccggcttgc
    10801 agtcgggtca aacgccgata ttctggtctt gaatgatgac ctgaccgttg agcggacctt
    10861 tatccggggc gttgaggaat accatcgggt ttaaccgccg tccggcaccg cctttggtta
    10921 t
//
LOCUS       DATALY010000273         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_664239,
            whole genome shotgun sequence.
ACCESSION   DATALY010000273 DATALY010000000
VERSION     DATALY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664239"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VHR42_07540"
     CDS             <1..609
                     /locus_tag="VHR42_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3027064.1"
                     /translation="ILGFINLQAQGVRQELVTAGVDTVSDKLDRLVEVTQAAHDEIRA
                     YIKSARSISHSDSDFLESLRNDILHFEQRSGIPTKLFTNLDLTEIDLAPQVQLHLLSI
                     AKEALNNVQKHSGANHVKLSLSLEKEKILLSIEDNGRGFNVSDKNDSAGKAFGLSIMK
                     ERAMEIGGKLAIESESGKGCRVALTLPVSQGGKNVHETHVGG"
     gene            587..1261
                     /locus_tag="VHR42_07545"
     CDS             587..1261
                     /locus_tag="VHR42_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3027065.1"
                     /translation="MKLMLVDDHPLFIEGLIYLLKTYGFEVAGVAHSGREALEKARLL
                     KPDVILMDIKMPDLSGIDTLKLLKAESPEIKIAMLTSSEEDEDLFDAVKYGASGYLLK
                     STGAKELVELLHGLENGEVLLTPPFAAQILRELRRTNSGEKERTGQSEKQASEEPQLT
                     GRQLEILELVAKGVTYKEAGAALGLAERTVKYHMGKIIELLHMENRSQVIAYAVKMGI
                     VQESHL"
     gene            1458..1705
                     /gene="tnpA"
                     /locus_tag="VHR42_07550"
                     /pseudo
     CDS             1458..1705
                     /gene="tnpA"
                     /locus_tag="VHR42_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1705..1927)
                     /locus_tag="VHR42_07555"
                     /pseudo
     CDS             complement(1705..1927)
                     /locus_tag="VHR42_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          545 a          418 c          515 g          449 t
ORIGIN      
        1 attctggggt ttatcaatct gcaggcgcag ggcgtccggc aggagcttgt gacggccggc
       61 gtcgataccg tcagcgataa actggacagg cttgtggaag tgactcaggc ggcgcacgac
      121 gaaatccgcg cgtatatcaa aagcgcccga agcatttctc actctgacag cgattttctg
      181 gagtctttac ggaacgacat cctgcatttt gagcagcgga gcggcatacc gacgaaactt
      241 tttacaaacc ttgatctgac cgaaatcgac cttgcgccgc aggttcagct tcatctgctc
      301 agcattgcga aagaggccct caacaacgtg cagaagcatt ccggggcgaa tcatgtgaaa
      361 ttgtctctgt cgcttgaaaa agaaaaaata cttttatcga tagaagacaa cggacggggc
      421 tttaatgttt cagacaaaaa cgacagcgcc ggaaaagcgt tcgggctgag catcatgaag
      481 gagcgggcga tggaaatcgg tgggaagctt gccattgaat ccgagtcggg aaaaggctgc
      541 cgggtcgctt tgacgttgcc cgtttcacag ggagggaaaa acgtccatga aactcatgtt
      601 ggtggatgac caccctttgt ttatcgaagg tcttatctat ctcttgaaaa cctatggctt
      661 cgaggtcgca ggcgtcgccc attcaggcag ggaagcgctt gaaaaagcaa ggcttctgaa
      721 acccgatgtc attttgatgg atattaaaat gccggacttg agcgggatcg acacgctgaa
      781 gcttttaaaa gccgaaagcc ccgaaattaa aatcgccatg ctgacgtctt cggaagagga
      841 cgaggacttg tttgatgcag tcaaatacgg ggcgtcaggt tatctgctga aaagcaccgg
      901 cgcaaaggaa ctcgtggagc tgctacacgg ccttgaaaat ggggaggttc tccttactcc
      961 gccgtttgct gcgcagattc tccgggaact gaggcggacg aattcaggtg agaaagaaag
     1021 gacgggccaa tccgagaaac aggcttctga agagccgcag ctcaccggac ggcagcttga
     1081 aattctggag ctggttgcaa agggagtcac ctataaggaa gccggcgcgg ctttggggct
     1141 ggccgagcgt acggtcaaat accatatggg aaagatcatc gagctgctgc acatggaaaa
     1201 ccggtcgcag gtgatcgctt acgccgtaaa aatggggatc gttcaggaat cccatttgta
     1261 aggggaatac gtccatcgct caaaaaagta cggggatttt taaaaatcca ccgtactttt
     1321 tgtattacga aaacccccgg ctatgccggg gtgttcagaa aaagctttag cgttccatat
     1381 aataaaaagc tccttttgat ataattaaac tgcggtctgt caactgcaat agaacatatc
     1441 aaaaggagaa catcagaatg aaatctaatg ataataacag tttagctcat acaacatgga
     1501 attgcaaata ccatatcgta tttgcaccaa agtacgccga aaagtagcat atgggaaaat
     1561 gagggctgag atagggaaaa tactgagaga gctatgtgat tggaagcagg ttgagatcgt
     1621 agaagcggag gtatgtcccg atcatattca catgctggtc aaggtaccac cgaagatgag
     1681 tgtttcggga ttcgtaggat ttctaaacag atccattata gcggccagat acagccttcc
     1741 ttcccgtgtg ctgatatagg tgatatcgga aacccacgcc acattaggct gtttcacttg
     1801 aaattcacga ttaagcatat taggagcgac gggatagtca tggttggaat aagtggtcgc
     1861 tttaaatctc cgaatcgttt tcgggatcat gttgttttca accataatat ctcgtacacg
     1921 agttctg
//
LOCUS       DATALY010000274         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_575487,
            whole genome shotgun sequence.
ACCESSION   DATALY010000274 DATALY010000000
VERSION     DATALY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575487"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VHR42_07560"
     CDS             <1..152
                     /locus_tag="VHR42_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX3027066.1"
                     /translation="MEALTGVSVALLTIYDMCKAIDKQMVMTDIHLVNKTGGKSGEFY
                     WNGGR"
     gene            155..1129
                     /gene="moaA"
                     /locus_tag="VHR42_07565"
     CDS             155..1129
                     /gene="moaA"
                     /locus_tag="VHR42_07565"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX3027067.1"
                     /translation="MKDTFNRKIDYLRISVTDRCDLRCKYCMPERGVRSLTHRDILSY
                     EEILRITERFARLGISRIKITGGEPLIRHNVVDLIQKIKSIQGIEKVTLTTNGTRLSN
                     FIDQLSMIKVDGINLSLDTLNPELYEKITGRNAFQKVFDGFQKALEYPDIKLKINCVP
                     MGIPEQNLVELAGLAKNHPVHVRFIEMMPIGLGRRFPFKSETDTMRELEQAYGTLTPF
                     DEVLGSGPCRYYSISGFQGKIGFISAMSHKFCSTCNRVRLTADGKLKTCLQFDVGVNL
                     KEIVRGGGSDQELEAAILKALENKPMEHHFTEDFVADRETRSMFQIGG"
     gene            1182..1673
                     /locus_tag="VHR42_07570"
     CDS             1182..1673
                     /locus_tag="VHR42_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX3027068.1"
                     /translation="MKRAAIITSSDSGYRGEREDLSGKVIKEIVELNGYEVVSMTLLP
                     DEQKMLSEAMAEIADQNKAELILTTGGTGFSQRDCMPEATRAVIEREVPGIPEAMRAF
                     SAQYTKRAMLSRAAAGIRKSTLIINLPGSPKAVRECLEFIIPELGHGIEILTGEANNC
                     ARQ"
     gene            1718..2146
                     /locus_tag="VHR42_07575"
     CDS             1718..2146
                     /locus_tag="VHR42_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX3027069.1"
                     /translation="MGKVIAVCVSDVKGVQKTNVHTAQFIEDYGIEKDAHAGKWHRQV
                     SLLSFEKIEDFKAKGADVRDGDFGENLIVQGIDFKNCPIGTRFRCNDVVLELTQIGKE
                     CHKGCAIFQVMGDCIMPREGVFTRVIHGGKISEGDELVII"
     gene            2442..3320
                     /locus_tag="VHR42_07580"
     CDS             2442..3320
                     /locus_tag="VHR42_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3027070.1"
                     /translation="MKKTLAIGLSILSAITMLAGCGAKQTPKSASSAAQSAVAQKGVL
                     LMSTTTSTQDTGLLDYLAPLFLKDTGWKLQWTAVGTGEALKMGQNGDVDIVLCHAKAS
                     EEDFVSKGYGVKRYPVMYNDFVVVGPAEPIPATKDIKSVFTQIHSKSLPFVSRGDDSG
                     TDKAEKKIWAGLKIDPKTNKNYIQSGQGMGDTITMANEKKAYCFTDRGTWLKMKKSST
                     VSIDLDIICQGDPKLKNQYGVIAVNPAKFPKVNNAAANEFINWICSARIQKLIGEYGL
                     DKYGQALFTPNAGTNS"
     gene            3393..>4002
                     /locus_tag="VHR42_07585"
     CDS             3393..>4002
                     /locus_tag="VHR42_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027071.1"
                     /translation="MNMIKEAVNGLFANIGILSAVRVTLIMATFSTLISSVLGILLGF
                     LLEQHRFPGKRIVIRINRTLLGIPPVVVGLIVYLLLMRKGPLGALSLLFTIRGMIIAQ
                     TIIITPIITSMVYSYAVKAAPAVRMFATTMGANNRQAKILLMKEMKNEIYFAVVTGFG
                     RSISEVGSVMLVGGNIEDRTRTMTTAISMLKSEGIFTQGVTLG"
BASE COUNT         1126 a          895 c         1081 g          900 t
ORIGIN      
        1 agatggaggc gcttacggga gtttcagttg ctcttttaac gatctacgat atgtgcaaag
       61 ccatcgacaa acagatggtc atgacggata tccacctggt gaataagacc ggagggaaaa
      121 gcggagaatt ttactggaat ggcggcagat aataatgaaa gatactttta acagaaaaat
      181 cgattatttg cgtatttcgg taacagaccg atgcgatctc cggtgtaaat actgcatgcc
      241 ggagagaggc gtgaggtcgc ttacccatcg ggatatcctg tcctatgaag aaatcctgcg
      301 gatcacggaa cgctttgcgc gccttggaat cagccgcatc aaaattaccg gcggagagcc
      361 gctgatccgt cacaatgtgg ttgatctgat tcagaagatc aaaagcatac agggaatcga
      421 aaaggtaaca ctgactacga acggcacacg gctgagcaat tttatcgatc agctgtccat
      481 gataaaagtt gacggtatca acctcagtct ggatacgtta aacccggagc tttatgaaaa
      541 aatcacgggc cggaatgcct ttcaaaaagt ttttgacggg tttcaaaaag cgctggagta
      601 tccggatatc aaactgaaaa tcaactgcgt gcccatgggt atcccggagc agaatctcgt
      661 cgagttagcc gggcttgcaa agaatcaccc tgtccatgtc cggtttatcg agatgatgcc
      721 gatcgggttg ggcagaaggt ttcccttcaa aagcgagacg gatacgatgc gggagctgga
      781 gcaggcttac ggaacgctta ccccttttga cgaagtgttg ggcagcgggc cctgccgcta
      841 ttacagcata tcaggctttc agggcaaaat cgggtttatc agcgcgatga gccataaatt
      901 ctgcagcaca tgcaacaggg taaggctgac ggcggacggg aagcttaaaa cttgcctcca
      961 gtttgatgtc ggtgtcaacc tcaaggagat cgtaagaggc ggcggctccg atcaggaatt
     1021 ggaagccgcg attttaaagg cactcgagaa taaaccaatg gagcaccact ttacagaaga
     1081 ttttgtcgca gaccgggaaa caaggtcgat gttccagatc ggcggataaa ttcgccggcc
     1141 ggccaaagct tccggagctc agcgaaagga tggtcatctt tatgaaacgc gcggcgatta
     1201 taacgtccag cgattccggt taccggggcg aaagggaaga cctcagcggg aaagttatca
     1261 aagagatcgt ggagctgaac gggtatgagg tggtttccat gacccttttg ccggatgagc
     1321 agaagatgct gagtgaagca atggcggaaa ttgccgatca gaataaggcg gagctgattc
     1381 tcacgacagg gggaaccggt ttttcccaaa gagactgtat gcccgaagcg accagggcgg
     1441 ttattgagag agaggttccc ggtatcccgg aagcgatgag agctttcagc gcgcagtata
     1501 ccaaacgggc tatgctgagc cgcgcagccg ccggaatccg caaatccacc ctgatcatca
     1561 atctgcccgg cagcccgaag gccgtgcggg aatgcctgga gtttatcatt ccggagcttg
     1621 ggcacgggat tgagatactt acgggcgaag cgaataattg tgccaggcaa taaagaggtt
     1681 ggcgtgaaaa aacttcacgc tatttaagga gataacgatg ggaaaagtaa ttgccgtatg
     1741 tgtaagcgac gtcaagggcg ttcagaaaac gaatgtccat accgcacagt ttattgaaga
     1801 ttacggaatt gaaaaagacg cccacgcggg caaatggcac cggcaggtaa gccttctctc
     1861 ttttgagaaa atcgaggatt ttaaagcgaa aggcgccgac gtcagggacg gggactttgg
     1921 ggaaaatctg atcgttcagg gaattgactt caaaaactgc ccgatcggga caaggtttcg
     1981 ctgcaatgac gtcgtgctgg agctgacgca gatcggcaag gagtgccaca agggctgtgc
     2041 aatttttcag gttatgggcg actgcattat gccgcgtgaa ggagtgttta ccagagtcat
     2101 ccacggtggg aagatatccg agggtgatga gcttgtcata atctgatctt taggaaatat
     2161 aatacagtgt attgagctgg aataatgaga taatatatac aactgaatca ataattaata
     2221 taggagcagt tgtatatagg gaacggctaa acgcaaaagc acgcgcaaaa ttttgctacg
     2281 caaacgcgca tgtgcaatat ataacagaat ggccaactcc tgtcggcaga tatactaaaa
     2341 acttattctg ttatatatag tgatatgttt gtcttttaaa ggaacggcgt tttccgatct
     2401 cattatttcg ttgataacaa caagcttaga gggtgaagag cgtgaaaaaa acactggcga
     2461 ttgggctttc tattttatcg gccatcacca tgcttgccgg ctgcggagca aagcaaaccc
     2521 cgaaatcggc ctccagcgca gcacagtccg cggtagcgca aaagggcgtg ctgcttatgt
     2581 ctacgacgac aagcacacag gataccgggc tgcttgatta tctggctccg ctgtttctga
     2641 aggataccgg ctggaagctt cagtggacgg cagtcggcac cggcgaagct ctcaaaatgg
     2701 gccagaacgg cgatgtcgac attgtgctgt gccacgccaa agcaagcgag gaagacttcg
     2761 tgtcaaaagg ctacggcgta aagcgttacc ctgtgatgta taacgatttt gtcgttgtcg
     2821 gccccgccga accgattccg gcaacaaaag acatcaagtc ggttttcacg cagatccaca
     2881 gcaagagcct gccgtttgtg tctagaggcg atgattccgg cacagacaag gcggaaaaga
     2941 agatctgggc aggcctgaaa atcgacccaa agacaaataa aaattatatt cagtccggcc
     3001 agggcatggg cgatacgatc accatggcga atgagaaaaa agcctactgc tttacagacc
     3061 gcgggacatg gctcaaaatg aaaaaatcat caaccgtgtc aatcgatctc gatattatct
     3121 gtcagggcga tccaaagctg aaaaaccaat acggggttat tgcggtaaac ccggcgaaat
     3181 tccccaaagt aaacaatgcg gccgccaatg agtttatcaa ctggatctgt tctgccagga
     3241 ttcagaaact gatcggcgaa tacgggttag acaaatacgg gcaggctctg tttacaccga
     3301 atgccggaac gaattcatag aaaacggttt tgaggggatg cttttcaagt atcccccctt
     3361 tcttatgccg ttaatacagt cacggtgatt aaatgaacat gataaaggaa gcagtgaacg
     3421 gcctgttcgc taatatcggc atactcagcg cggtcagggt aacgctgatt atggcgacat
     3481 tttcaacttt gatttcttct gtgctgggca tccttctcgg attcctttta gagcagcatc
     3541 gttttcccgg caagaggatc gtcatccgaa tcaaccgcac gctgcttgga atcccgcccg
     3601 ttgtcgttgg gctgatcgtt tatctgctgt taatgcgaaa aggccctctg ggggcacttt
     3661 cgcttctttt tacgataaga ggcatgatta tagcgcaaac tattatcata acgcccatta
     3721 tcaccagcat ggtgtattcc tacgccgtaa aggctgcccc ggctgtccga atgtttgcca
     3781 cgacgatggg agcaaacaac cggcaggcaa aaatcctgct catgaaagaa atgaagaatg
     3841 aaatttactt tgccgtggtt acaggctttg gccgctccat cagtgaggta ggctccgtaa
     3901 tgctggtcgg cggaaacata gaagaccgca caagaaccat gaccacggcg atctccatgc
     3961 tgaaaagcga gggaatcttt acgcagggtg tgacgctggg aa
//
LOCUS       DATALY010000275         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_834400,
            whole genome shotgun sequence.
ACCESSION   DATALY010000275 DATALY010000000
VERSION     DATALY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834400"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(448..1047)
                     /locus_tag="VHR42_07590"
     CDS             complement(448..1047)
                     /locus_tag="VHR42_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEX3027072.1"
                     /translation="MSKILYIKANPKPDAESRTFRISEAFIETYRRSHPDDEIVILDL
                     YREGIVPLSREDLRLHVPKQGESYENTYLNYAYQFVGADKYVIAAPMWNLGIPSILKA
                     YIDYISVTGITFHYTAKGPEGLCRGKKMVHITTTGGDYSSPQTAGYEMGDRYLRTIMG
                     FLGITDFSTIRAEKLDTGIDVEKAVAEAVQSAQSLAEIF"
     gene            complement(1155..1508)
                     /locus_tag="VHR42_07595"
     CDS             complement(1155..1508)
                     /locus_tag="VHR42_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3027073.1"
                     /translation="MIPGYDYFGYETKSQNTPISFPEQHQTKQPGIESEMNPAPIYDN
                     PEVKGSGKLEDCCALIPSSFTGEEVKSFGLSVPMKRAAQPFELAGAYVYLAGCDSSYV
                     TGQVIHVNGGQMNGS"
     gene            complement(1781..2764)
                     /locus_tag="VHR42_07600"
     CDS             complement(1781..2764)
                     /locus_tag="VHR42_07600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3027074.1"
                     /translation="MTFQEISKIVKEIKEKKAKVGGIKEVYFVACGGSLAANYPAKFF
                     LESEAKDLKVGYYTSNEFVHATPNALGKNSIAIVSSHQGTTPESVEAAKLSQEKGAST
                     ICFTFQKEAALAKYGDYVIGYEFGEKKDSANEKDAIILKVAVEILNQVEGYDKYDAVV
                     EGFGKLDNIIKKATKHVEARALEFAEAYKDEKLIYTMASGSSYGAAHIESICIFMEMQ
                     WINSSSIHSGEYFHGPFEITDANVPFIMLMSEGRTRALDERALAFLSKYGKKYEVLDA
                     KELGINEISDNVVEFFNPFLFSNVLGVYNKALAAARKHPLSTRRYMWKLKY"
     gene            complement(3114..>4267)
                     /locus_tag="VHR42_07605"
     CDS             complement(3114..>4267)
                     /locus_tag="VHR42_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3027075.1"
                     /translation="RFLFLASGFVHRLFWIPAALIPLFIIPNQHHAVIWSVTILITVS
                     SVAYSVNMVAFNSWMGDLVPEEIAGRFFGIRNLVGNITGGIAALALGVYLDRVNNFNG
                     FAIVFLVGVLFGALEVVAYFFIDHPPMALDEEKPSLLYILTEPLKNGNYRKFVLFATL
                     FAFSVNISMPFFNVYMIENLKMSYLSIGLADNIAFSIGAVIMVHRWGVLSDRFGNKPV
                     VMVNGLMIASIPILWLFVNPSNSFAIVFLSNFVSGIVWSGYNLTIFNQSVWLAPEKNR
                     SAYIASYTLFTSVIGTALANVCGGYFMQTFGSWINLLRLPFIAGETLNSYQVLFVCSG
                     ILRILSVLLFLPMVYEKNSASAKTMIRETTGSLRNITIRKKRSKNDAKK"
BASE COUNT         1198 a          934 c          906 g         1229 t
ORIGIN      
        1 aaaaggtgtt tttatccgat ccattttaga atgtatgagt tgactgtaaa agaggatgat
       61 aaagcaatgg tagaatgatt acaacatcat aggaaaaata agaggggttt gttttggaag
      121 aatcatagtg ttatgaaata acgcgcttga taggcatata ccaacgtgtg ataagatagg
      181 atgaatcgta gaatgtttag ttggattctg gtggattcag cgatacactt ctgtatagag
      241 cgcagaaaga attttaaggc tctttctgtt ctttctatga ttttattata tcgtttttag
      301 caataatgtc aatattcttt acctaatttc gtttgatgat acaaataact taaaatgtcg
      361 ttcagaataa tatgaatttt ttataaataa ttaccgattt ttatcgaagt catttggtgg
      421 aatattacgg taaaacgtgc tcgggcatta aaatatttcc gccagagatt gagccgactg
      481 tactgcttcc gccacggctt tctccacatc gataccggtg tcgagttttt cagcacggat
      541 tgtcgaaaaa tctgtaattc cgagaaatcc catgatcgtt ctcagatagc ggtcgcccat
      601 ttcatagccc gcagtctgcg gcgaggagta atcccctccc gtggtggtga tgtggaccat
      661 ttttttaccc cggcaaaggc cttccgggcc cttagcggta taatgaaagg tgattcccgt
      721 cacactgata taatcaatat aagctttgag gatggacgga atacccagat tccacatggg
      781 agccgcaatt acatacttat ccgccccgac aaactgatag gcgtaattca aataggtgtt
      841 ttcataggat tccccttgtt ttggaacatg aagccggagg tcctcgcggc tcagaggaac
      901 aattccttcc cggtagaggt caaggatcac aatttcatca tccgggtggc tacgacgata
      961 ggtttcgata aaagcttccg aaatccgaaa ggttctggac tcggcgtcgg gcttgggatt
     1021 ggctttgata tataaaattt tgctcatatt ttttccttct ctcttgtttt taaggatgtc
     1081 tttttttaat aatggaatac tttttgatta ttttacactt atttttttaa ataatcaacg
     1141 acaggcccgg gctctcacga cccgttcatc tgcccgccgt taacatggat cacctgcccg
     1201 gtcacataac tcgagtcgca gccggccaga tagacgtagg cgccggccag ctcaaacggc
     1261 tgagccgctc ttttcattgg tacgctcaga ccaaacgatt tgacttcttc cccggtaaag
     1321 cttgagggaa tcagcgcgca gcagtcttcc agcttgccgg aaccctttac ctccggatta
     1381 tcatagatgg gcgcgggatt catctccgac tcgatcccgg gctgtttggt ctgatgctgc
     1441 tccgggaaag agatcggcgt attctgactc ttggtttcat aaccaaaata atcataaccg
     1501 ggaatcatgc gatcacctct gtcattaaac ttcctttatt attcactccg ttcggcaatc
     1561 tattcgcatc cggccgccgc tgtaatacat tttacctctt tgccttttag aatcggatgt
     1621 tgcaacttaa ttataatttt ctcataaacc tgccgttacg tcggcttaca cttgccatga
     1681 gttcaatgtt tttttattta caattcattc atatataacc aaacagagcc cccgttcctt
     1741 tcggaacggg ggctctcatc tgaaataata tgtctgagaa ttagtatttc agtttccaca
     1801 tgtatcttct ggtggaaagc ggatgttttc tcgcagcggc cagagctttg ttgtaaacgc
     1861 ccagcacatt ggagaacagg aacggattga agaattctac gacattgtcg ctgatctcgt
     1921 tgatgccgag ctctttggcg tcgagaactt catatttttt gccgtatttg ctcaggaaag
     1981 caagtgctct ctcatcaaga gctcttgttc tgccttcgct cataagcatg atgaacggaa
     2041 cgttggcatc agtgatttca aacgggccgt ggaaatattc gcccgagtga atgctggaag
     2101 aattgatcca ctgcatctcc atgaaaatgc aaatggattc aatatgagca gcgccgtagc
     2161 tggaaccgga agccatggta tagatgagct tctcgtcctt gtaggcctct gcgaattcga
     2221 gtgctcttgc ctcgacgtgt ttcgtagctt tcttgataat attgtcaagt ttgccgaagc
     2281 cctctaccac agcatcatac ttgtcatagc cctcaacctg attgaggatt tcaaccgcga
     2341 ctttaagaat gatagcgtct ttttcattgg cagaatcttt cttttcgccg aactcatagc
     2401 cgatgacata atcgccatat ttggcaagag ctgcttcctt ctggaaagta aagcaaatgg
     2461 tgcttgcgcc cttctcctgg ctgagtttcg cggcctcaac cgactccgga gttgttccct
     2521 gatgggagct gacgatggcg atggagtttt tgcccagagc attcggggta gcatgaacga
     2581 attcattgct ggtgtagtag ccgactttta aatcctttgc ttcgctctca aggaaaaact
     2641 tggcaggata gtttgcagca agagaaccgc cgcaggcaac aaaataaacc tctttgatac
     2701 cgccgacttt ggcttttttc tctttgattt cctttacgat ttttgaaatt tcctgaaagg
     2761 tcatcgaaat tcctcctgtt ttttgaatga ttttagactt gaacccgagc aaaatattat
     2821 ccgtttagta taatatttta gatactcagg tttccacaac gcgtctttat aatagcacac
     2881 tcaatatgag aagtaaatag tttgagattg aaaaaagacg aattttttgc aataattatt
     2941 tctctgaatt ttagatataa tgccaatcta atagtacaaa attttaattt atcggacaaa
     3001 tagaacaatg agtttgagtt tttgaaataa ttttttattg tattgaagtc tgtataataa
     3061 cctatctttt agagcggatt tcatatgttg tgccggttaa tccaataaat gttttatttt
     3121 tttgcatcat ttttggaacg ttttttacgt atagtaatat ttcttaatga gcccgtcgtt
     3181 tcccggatca ttgttttagc ggaggccgaa tttttttcat aaaccattgg gagaaatagc
     3241 aaaactgaca ggatccgcag aattccggaa cagacaaaca gcacctgata ggaatttaag
     3301 gtttctcctg cgataaacgg cagacgcagt aaattgatcc acgacccgaa ggtctgcata
     3361 aaataaccgc cgcagacatt tgcaagggcc gttccgatca ctgaggtaaa cagcgtataa
     3421 ctggcgatat aggccgagcg gtttttctcc ggagccagcc agaccgactg gttaaatatc
     3481 gtcaggttat agccggacca tacaatcccg ctgacaaaat tcgacaaaaa cacaattgca
     3541 aatgaattgg agggatttac aaacagccat aaaatcggaa tggaagcaat catcaggcca
     3601 ttgaccataa ccaccggctt atttccgaaa cggtcggaca gcacgcccca tcggtgcacc
     3661 ataatgacgg cgccgatact aaacgcgata ttatccgcca gcccgatgga caaataactc
     3721 atttttaaat tttcaatcat gtaaacattg aagaaaggca tcgaaatatt gacgctgaat
     3781 gcgaataacg tcgcaaacag aacaaattta cgatagttgc catttttgag cggttcagtc
     3841 aaaatataga gaagcgaagg cttttcttcg tcgagcgcca tcggcgggtg gtcgataaag
     3901 aaataagcga ccacttcaag agccccgaac agcacgccca ccagaaaaac gatggcaaac
     3961 ccgttaaagt tattgacccg gtcaagataa acgcctaatg ccagcgccgc aattcctccc
     4021 gtaatattgc cgacaagatt tcgaattcca aaaaacctgc ccgcaatttc ctccggaacc
     4081 aggtcaccca tccaggagtt gaaagcaacc atattgaccg aataggcgac agatgaaacg
     4141 gtaatcaata tggtcaccga ccaaattacc gcatgatgct gattgggtat gataaacaga
     4201 ggaatcagcg cagcgggaat ccagaacagc cgatgaacaa aaccggaggc taaaaacaaa
     4261 aacctgc
//
LOCUS       DATALY010000276         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_665206,
            whole genome shotgun sequence.
ACCESSION   DATALY010000276 DATALY010000000
VERSION     DATALY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665206"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VHR42_07610"
     CDS             complement(<1..1087)
                     /locus_tag="VHR42_07610"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX3027076.1"
                     /translation="MRKRFFRAAGLSIIIGLIAAFLFAVPLIGKVYTDETEKRLDAAL
                     SLMVGYSLPEKPDYQVMAKEAGRNLKENNQDLRITIIDENGTVLGDSEADPAQMENHA
                     SRPEIIQALQSGKGKDIRRSFTVGQKEMYRANLQTMPDGSHVIYRTSIELKSVDQVHS
                     LLWGCGLIGIFIGLMVALATANYSAGRVVKPLQQLTQAARRLAEGSEAVRVEEAPDEM
                     GELSSAFNRMSERLSSAHIALEQSNERMASILQGMEDGVIALDTDGDITLLTRRAREL
                     LGPSPQNAIRLSDCGTNYSYIQNLMDRVAAGESPLRETIVLAGKPERILQVYTVKVSG
                     STAGGTLAVLSDVTRMRKLETMRSEFVA"
     gene            complement(1138..1836)
                     /locus_tag="VHR42_07615"
     CDS             complement(1138..1836)
                     /locus_tag="VHR42_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3027077.1"
                     /translation="MPMVYIVDDEPHIRRLAAIGLKDAGFDTAEFSDGATLLHAMRQA
                     LPDAVLLDWMLPGQDGINVLRTIRQDSSMRPTPILMMTARSDEVDRVLGLEFGADDYV
                     TKPFSVKELAARVKAVIRRNEYLNTPQSDTLSGGGLRVDNARRSVTKNGEPVELTQRE
                     FDLLYTLMKSPGRVFTREMLLSTVWKTEFFGDTRTVDVHIRYLRQKLEEEPDAPRLIL
                     TVRGVGYRFCDTTE"
BASE COUNT          458 a          565 c          421 g          483 t
ORIGIN      
        1 tggcgacaaa ctcgctgcgc atggtttcta gtttcctcat ccgggtcaca tcggataaca
       61 ccgccagtgt tcctccggct gtgcttccgc taaccttgac cgtatatacc tgaagaatgc
      121 gttcgggctt acctgccaac actatcgtct cccgaagcgg ggactctccg gccgcgactc
      181 tatccatcag attttgtata taactataat ttgtcccaca gtcggaaagg cgtatggcgt
      241 tctgcggaga agggcctaaa agttctctgg cacgtctcgt gagcagggta atatctccat
      301 cggtatctag ggcaatcact ccatcctcca taccctgcaa aatgctggcc atccgctcgt
      361 tactctgttc caatgcaata tgagcagagg acagccgctc ggacatacgg ttaaatgcac
      421 tggacagctc ccccatttca tccggagctt cctccacacg aaccgcttcg gaaccttccg
      481 ccagtcggcg agccgcctgc gtcagctgct gcagaggttt tacaacccga cctgccgaat
      541 aattcgcggt ggcaagcgcg accatcaatc cgataaaaat accgatcagc ccgcagcccc
      601 acaaaagact gtgcacctgg tcgacgcttt tcagctcgat agaagtacgg taaatgacat
      661 gggaaccatc cggcattgtc tgcaaattcg cccgatacat ctccttctgg cccactgtaa
      721 agctgcggcg gatatctttt cctttcccgc tttgaagcgc ctgtattatt tcagggcggc
      781 tcgcgtgatt ttccatctgg gcaggatccg cttcgctgtc cccaagtacc gttccatttt
      841 catcgataat ggtaatgcgt aaatcctgat tattctcctt taggtttctg ccggcttctt
      901 ttgccatcac ctgataatcg ggcttttcgg gcaatgaata gccaaccatt aaagacagag
      961 cagcgtctag acgtttctcc gtttcatcag tatatacttt tccaatcagc ggcaccgcaa
     1021 ataaaaaagc ggcgataagt ccgataataa tggacaaacc tgccgccctg aaaaatctct
     1081 tgcgcataaa ttatgacctt cctttatgcc gtagcaggga caaaatccct gctttttcta
     1141 ttccgttgta tcacagaagc ggtaaccaac gccgcggacc gttaaaatca gccggggagc
     1201 atccggttcc tcctccagct tctggcgcag ataacggatg tggacatcaa cggtacgggt
     1261 atccccgaaa aattccgttt tccaaaccgt actaagcagc atttcacggg taaaaacccg
     1321 tcccggtgat ttcatcagtg tatagagcaa atcaaattcc cgctgagtta attctaccgg
     1381 ctccccgttt tttgtaacgc tgcggcgggc gttatctaca cgcagccctc ctccggacag
     1441 ggtgtcgctc tggggcgtat tcaaatattc attacggcgg ataaccgctt tcacacgtgc
     1501 cgccagctct tttacgctga aaggctttgt tacataatca tctgcaccga attccagccc
     1561 aagcacgcgg tcaacttcat ccgaccgcgc ggtcatcata agaatcggag taggccgcat
     1621 tgaggaatcc tgccgaatgg tgcgcagcac gtttatccca tcctgtcccg gcagcatcca
     1681 gtcgagtaaa accgcatcgg gcaatgcctg acgcatcgca tggagtaacg tcgccccatc
     1741 ggaaaactcc gccgtatcaa aaccggcatc ttttaaacca atagcggcaa gccgacggat
     1801 atgaggctcg tcgtcaacga tatataccat cggcataaaa cccctcccct tctttcaaat
     1861 agcctatttt acatcttgat tataaagcgt ctttttcctc ttgtccagca cccgcctgcg
     1921 tatcatt
//
LOCUS       DATALY010000277         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_236107,
            whole genome shotgun sequence.
ACCESSION   DATALY010000277 DATALY010000000
VERSION     DATALY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236107"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..432
                     /locus_tag="VHR42_07620"
     CDS             94..432
                     /locus_tag="VHR42_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027078.1"
                     /translation="MADKNLEEAKKLNQQSRQGNMSNSVSGSDQGVEDAKQRNQQSAS
                     SVSNQGKINNAVNGDPLQEARDANEQSASAQSSTGSSSSFSFSSGSSQDSDLESAKKE
                     NRKSGQNKGQ"
     gene            531..1478
                     /locus_tag="VHR42_07625"
     CDS             531..1478
                     /locus_tag="VHR42_07625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX3027079.1"
                     /translation="MENKSRNWFREILISHTTEQAESLFIVGTPSTTPELSEQKEKHP
                     QPWLFARVLLIVFMLYFGFYVGLTFFQNVNFLPGLIILGAFLVPLSILVFFWEMNLPC
                     NISLYRVIMFFMGGGLVSLLYSVILYTFTDGYTNTLVIGFVEETAKLFALLTFAARRR
                     YPHILNGILIGAAVGAGFSAFESSGYILMTALQYGVKVMLNTIFWRAILSPGSHIAWA
                     GLMGAAVMMVKGKGRFKFRSLFHFRFIGIYLLVILLHALWDTDFPQPFFRALPLYPII
                     LTILSWIGIVLFLKKGIRQIVQYPLENGTNPPEEKETAQ"
     gene            complement(1639..2253)
                     /locus_tag="VHR42_07630"
     CDS             complement(1639..2253)
                     /locus_tag="VHR42_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027080.1"
                     /translation="MLTENNYEKRYCPDRKKSRLSKWEKCLHHPALLREAEISEAFYE
                     LLVTIEENNWSGACHSASAILYVLFSELEIHAELCVGEVQFDTTIIDHSWVEANTEIY
                     DASIYRGICGISLSPPVFRSLNIETQKPADGVYGIAKDGIGETARSILSVSLSDYMDH
                     YTVCENGLWNYVREIGNRVGLSVDISRLRQKYKTTAWKYKKNLI"
     gene            2558..3820
                     /locus_tag="VHR42_07635"
     CDS             2558..3820
                     /locus_tag="VHR42_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3027081.1"
                     /translation="MRDETKCIHAGYTPKNADPKMVPIVQSTTYVFHSTEEVGDVFDE
                     PTKALIYSRFANPTVMAVEEKIAALEGGIGAMCTSSGQAATLLSILNICKAGDSFVST
                     TQIYGGTLNLFAVTLKNLGIECVFVDCGADEETIAAAIKDNTKAIFGETIANPALSVL
                     DIEKFAKVAHAHGIPLIVDNTFATPVLCKPIDFGADIVVHSTSKYMDGHAVQVGGVIV
                     DSGRFDWSNGKFPGFTEPDESYHGITYVSTYGKAAYIVKARMQLMRDYGVYPAANSAF
                     LLNLGLETLAVRMERYCQNALKVAKYFEASDKIEKVIYPGLESDPFYPLAKKYLKGPS
                     GVITLVIKGGKKNAVKFMDGLKLASIEVHVADIRTCVLHPASATHRQLTEEQLVAAGI
                     NPGMIRFSVGLENVEDIIEDIEQSLSKI"
     gene            complement(4173..>4543)
                     /locus_tag="VHR42_07640"
     CDS             complement(4173..>4543)
                     /locus_tag="VHR42_07640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3027082.1"
                     /translation="LEQNEAFAKEKELQDGWKVPTAQFWLYVDGRPVGIGRIRHFLTD
                     KLRQEGGQVGYAIRPGERNKGYGTLLLGELIKEARKMKIPEVLLTVHNGNEYSKKVAL
                     ANHGVIEKVSSERHYIRIPC"
BASE COUNT         1171 a         1011 c         1079 g         1282 t
ORIGIN      
        1 tgtaagtcat atgacttatc aattttcgaa aagtgctggc tttaaaatac gttaatatgg
       61 aaccgccgca tttgcggagg gatggaggtt aatatggctg ataaaaattt ggaagaggct
      121 aaaaagctga atcagcagtc ccggcaggga aatatgagca attctgtttc cggaagcgat
      181 cagggtgttg aagatgcaaa acaaagaaat cagcagtctg caagctctgt ttccaatcag
      241 ggtaagatta acaacgctgt aaacggcgat cctttacagg aagcaagaga cgcgaacgaa
      301 cagtctgcga gtgctcagtc atcgaccggt tcatccagtt cttttagttt ttcttcaggt
      361 tcctctcagg attctgatct tgaatctgcc aaaaaggaaa accgcaagtc cggacagaac
      421 aaaggtcagt aagcaataaa ggatataaaa aaacgaatca atctcctgtt gaacgattca
      481 atgggagatt gatttttaca gagcttaatt tcttttcgga agggttgatt atggagaata
      541 aatcaagaaa ttggttccgg gaaatcttaa tttcgcatac gacggagcag gcggaatcgc
      601 tttttatcgt cgggacgccc tctacaacgc ccgagctcag cgagcagaag gaaaaacacc
      661 cgcagccatg gctttttgcc agagtccttc tgatcgtttt tatgctttac tttgggtttt
      721 acgttggtct gacttttttt caaaacgtca atttcctgcc gggcttaatt atcctgggag
      781 cttttcttgt gccgctctct atcctggttt tcttttggga aatgaatctt ccctgtaata
      841 tatcccttta cagagtgatt atgtttttta tgggcggcgg tctggtttca ctgctttatt
      901 ccgtgattct ttatactttc accgacgggt atacgaacac gctcgtcatc ggttttgtcg
      961 aagagactgc gaaacttttt gcgctgctta catttgccgc ccgaagaaga taccctcata
     1021 ttctcaatgg aattttgatc ggcgcggcgg taggcgcagg attttctgcg tttgagagct
     1081 ccggctatat cttgatgaca gcgctccagt atggagtcaa agtaatgctc aacacgattt
     1141 tctggcgggc aattttatct cccggaagcc atatcgcctg ggccggactg atgggtgccg
     1201 cggtcatgat ggtgaaaggt aaaggcaggt ttaaattccg ttcgctcttc cactttcgat
     1261 ttatcgggat ttatcttctt gtcattttac tgcacgccct gtgggatacg gattttccgc
     1321 agccgttctt ccgggctctt ccgttatacc cgattatcct gacaatcctt tcctggatcg
     1381 gaatcgtact gtttttgaaa aagggaatac ggcagattgt tcaatatccg cttgaaaacg
     1441 gtacgaaccc gcccgaagaa aaagagacgg cgcagtgatt ggttattgac tcgttatcag
     1501 gatgatcttc aagagcttgt tagtaaaatg aaaaaattaa caatttagat tgtagacggc
     1561 atttaaaagc cggttttaac agtttctttg agaaatcaat cgtcagtagg cgcagagaag
     1621 gctattttca atcaagccct aaataagatt ctttttatat ttccatgctg tggttttgta
     1681 tttttgcctt aatcgggaga tatcgacgga taggccgact ctgtttccga tctcccgtac
     1741 atagttccat aaaccgtttt cacagaccgt gtagtgatcc atgtagtcac tcagtgaaac
     1801 cgaaagaatc gatcgggcag tttcgccgat tccgtctttt gcaataccat atacgccgtc
     1861 ggcgggcttc tgtgtctcga tgtttaaact tctgaaaacg ggcggagaaa gactgattcc
     1921 gcaaattccc cggtaaatcg aggcgtcgta aatttccgtg ttggcttcaa cccatgagtg
     1981 gtcaataatc gtggtgtcaa attgaacctc gccgacgcaa agctcggcat ggatttccaa
     2041 ttcgctgaaa aggacataga gaatggcaga ggccgagtga catgctccgg accagttgtt
     2101 ttcttcgatg gtaacaagaa gctcataaaa ggcctctgaa atttctgctt ccctcagcag
     2161 ggcggggtga tgaaggcact tttcccattt cgatagcctg cttttctttc tgtcagggca
     2221 atatcttttt tcataattat tttcagtcag catgttttct ccgctttcat aaattcctga
     2281 aagttcgatt tactttcagt ataccggaat cggaaacgga agtaaaacag aaatttctag
     2341 tatgcaaggt gaggctgcgg agtggatttt tcagacttac ttaaaaagca ttttgcatat
     2401 tcataaatga acgggctgaa ttcgttgcta tttatcgttt tatggggtta taatagaaat
     2461 taaatattca gaggatcaag ctcttttagc tttgacggaa ttctacaaat ttaattgctt
     2521 tgaatagcaa cagcaggtta caggagggac tattttcatg agagacgaaa ccaagtgcat
     2581 tcatgcaggc tataccccaa aaaacgcaga tcctaaaatg gtaccgatcg tgcaaagcac
     2641 cacgtatgta ttccactcca ctgaggaagt cggagacgta tttgacgagc cgactaaagc
     2701 tctgatttat tcccgttttg caaaccctac cgttatggcg gttgaagaaa agattgcggc
     2761 gcttgagggc ggtatcggcg cgatgtgcac ctcttccggg caggcggcta cgcttctttc
     2821 gattttgaat atctgcaaag ccggtgacag cttcgtaagt acgacgcaga tctacggagg
     2881 tactctcaat ctgtttgccg taacgctgaa aaacctcggc atcgagtgtg tttttgtcga
     2941 ctgcggtgcg gatgaagaga cgatcgccgc tgcaatcaaa gataatacca aggcgatttt
     3001 cggcgagacg atcgccaacc ccgccttaag tgtgcttgat attgaaaaat ttgcgaaggt
     3061 cgcgcacgca cacgggattc cgctgattgt tgacaatacg tttgcaacgc cggttttgtg
     3121 caaaccaatt gatttcggcg cggatattgt cgttcattcc acctccaaat acatggacgg
     3181 ccatgcggtt caggtaggcg gagtcatcgt tgacagcggc aggtttgact ggtccaacgg
     3241 caaattcccc ggttttaccg agccggatga gtcataccac ggcattacat atgtcagcac
     3301 ctatgggaaa gcggcgtata ttgtcaaagc aagaatgcag ctgatgcgtg attacggggt
     3361 ttacccggcg gctaattccg ccttcctgct gaatctcggg cttgagactc tggccgtgag
     3421 gatggaacgc tattgccaga acgcgctgaa ggtagcaaaa tatttcgaag catccgataa
     3481 aatcgaaaag gtgatttacc ctggtctcga aagtgacccg ttttatccgc ttgcgaagaa
     3541 atatcttaag ggcccgagcg gcgtgatcac actggtcatt aagggcggca agaaaaacgc
     3601 cgtcaaattt atggacgggt taaaactggc gtctattgaa gtacatgttg cagatatccg
     3661 cacctgcgtt ctccatccgg cgagcgcgac gcatcggcag ctgacagagg agcagctggt
     3721 cgcagccgga attaatcccg gcatgatccg cttctcggtt ggtcttgaaa acgtagagga
     3781 tattattgaa gatatcgagc agagcctctc aaagatctga ctttgattct gaattagttt
     3841 cattaggcgt ccgaaaacgg tcagattaac ggctgacttt ttcgggcgct ttttttcgat
     3901 caggcttttt acaggatgat atgaaactca ataaacagtc aggcacagat ggcagcgtat
     3961 tctgcacaac gggtcgggac gctatatcac ccggcagctg ctgaagtata agcatattgt
     4021 ggtagccacc atgcctctgc agcttaacga agaaggtctt ttattcacac gcgaacaact
     4081 tcattcataa tatttgaaat gcggtaacag gccggctgcc tgctaccgca tttatgatag
     4141 atatagctcc tcacagcact gcatcagata ttttaacaag gtatccggat ataatgccgc
     4201 tctgagctca ctttttcaat cactccgtga ttggcaaggg caactttttt tgaatattcg
     4261 ttcccgttgt ggacagtgag aagtacttca ggtattttca tctttcgggc ttcttttatc
     4321 agctcgccca gcagaagcgt accgtatccc ttgtttctct ccccggggcg tatcgcataa
     4381 ccgacctgcc cgccttcctg ccgaagctta tccgttaaaa agtggcggat tctcccgatc
     4441 ccgacgggac ggccgtccac atacagccaa aactgcgcgg ttggcacttt ccagccatcc
     4501 tgaagttctt tttctttagc gaaagcttca ttttgttcaa gcc
//
LOCUS       DATALY010000278         7026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_576742,
            whole genome shotgun sequence.
ACCESSION   DATALY010000278 DATALY010000000
VERSION     DATALY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576742"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VHR42_07645"
     CDS             <1..1572
                     /locus_tag="VHR42_07645"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3027083.1"
                     /translation="HEKLSPVLAYHVVDSTEAGFAAALRMLEIGGMGHSAVIHSEDQA
                     LIVKYGDAMKVGRVIANSPSSQGAIGDIYNTNTPSLTLGCGSYGHNSTTSNVSSVNLI
                     NKKRLAKRRVNMQWFKVPPKIYFEPNSVQYLEKMPDISRAFIVTDPLMVKLGYVDKIL
                     FYLRRRNEYCHSEIFSEVEPDPSVDTIMRGVETMNQFKPDVIIALGGGSAMDAAKGMW
                     LFYEQPQNDFNALRLKFMDIRKRAYHFPELGKKCQMVAIPTTSGTGSEVTAFAVITDK
                     TNNVKYPLADYALTPNVAIVDPQFVMTMPKTTTADTGMDVLTHAIESYVSVLASDYTD
                     GLAMKAIELVFDYLPRAYNDGSDQVAREKMHNASCMAGMAFTNAFLGLNHSMAHKLGG
                     EYHIPHGRANAILLPFVIAYNGSKPTKFTSWPKYESFVADEKYAQIARHLGLPASTAA
                     EGVQSLVKAVQDLLVKLNVPTSIRACGVDEATFYKNVEYLADRAFEDQCTTANPRLPL
                     VKEIVEIYKKAYNGN"
     gene            complement(1671..2135)
                     /locus_tag="VHR42_07650"
     CDS             complement(1671..2135)
                     /locus_tag="VHR42_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEX3027084.1"
                     /translation="MNQKNKFIAMAAAIAAIYCVLTLVFAPISFREIQFRISEIMTVL
                     PFFTPAAIPGLFVGCVLANIASPLGPIDMAVGGAASLIAAYLTRKMPNKALAPLPPVI
                     VNGIFVGVELHLIYKLPLLLTMLYVALGEAAVCYLLGLPFLFALEKLKGKLF"
     gene            2457..2969
                     /locus_tag="VHR42_07655"
     CDS             2457..2969
                     /locus_tag="VHR42_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027085.1"
                     /translation="MKLSRQVSKGISTGILILVLIFIYINFNTIFTKLNYNALAVTDN
                     QKDETINLPTQINFLSSDKKEFVEYCSLTFDVNLSDQSGNRVFTYYGAINGYRFYRLQ
                     LNCIPCDSAFTEENIGGYTFTSPCYYRPNPTGLYIIGDDGVYTLKEAYEKGLINISNA
                     YSLYQKKNIT"
     gene            complement(2962..3918)
                     /locus_tag="VHR42_07660"
     CDS             complement(2962..3918)
                     /locus_tag="VHR42_07660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3027086.1"
                     /translation="MYDLLREALERADIGIIIIDDSHSIRFINRYIEKLSGILYEQAL
                     GQNLAKLFPVFAGNRYQDILKKTLEQNQKRFCSSLIHHAFIYPQKNPGVDVRQNMQVE
                     PVTINGNVFVMLQVRDITDRVNNEMKLTSLINELKKGYLEIKESEEYIRKIAQTDLLT
                     GLLSRHAMMQIIGNVLKSNDIAGGYALMFLDLDGFKNINDTYGHLAGDQLLKKFAQRL
                     LLNTRKDDFIARLGGDEFLVLLSSIHSKQNAVHVAKKLLEDFKKPVEVENSPILITAS
                     IGIAMFDFTVRSVSEIIRRADFAMYQAKQMGKNTFVLFEDLK"
     gene            complement(3911..4540)
                     /locus_tag="VHR42_07665"
     CDS             complement(3911..4540)
                     /locus_tag="VHR42_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEX3027087.1"
                     /translation="MLSEMEKDVLVEVMNTNIGLAASLLSEMASQKVILSVPELDLKK
                     GNEIDFRDIDRNMADFSKSVMSTLRFGNRFSGNAYIVFPADKAKNLINACTGESSASE
                     DADFQKLSLEDLDVIKEISNIIFNAVIGEFGNLLDVRLEYTFPSIEMTMISDVENTIL
                     PEEMHFLSMYTSFLLSESQVKGVIFIALTMNSEKMLIDKIDEMLVELNV"
     gene            complement(4567..4926)
                     /locus_tag="VHR42_07670"
     CDS             complement(4567..4926)
                     /locus_tag="VHR42_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3027088.1"
                     /translation="MKILIADDSLFVRQFIRKFLLELNPGLELTFAASGEEGYQKFLN
                     DHFDVIITDLLMPGMGGEEFIKKVRLADRDIKVIVVSADIQKSVKDEILGLGVSAFLN
                     KPIKRENIQEIYEFIGR"
     gene            complement(5009..6031)
                     /locus_tag="VHR42_07675"
     CDS             complement(5009..6031)
                     /locus_tag="VHR42_07675"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX3027089.1"
                     /translation="MANIAVFGAGGWGTALSVMCAESGHRVSLWSPFENELESIRRDG
                     EHKKLLPGVRVPEKINLTSDAVVANGADVIVLAVPSFAVRQTAVRLKDYIRPGQVIVN
                     VAKGFEEETLSRLSQVIAEELPQAKVVILSGPSHAEEVSRGVPTTVVAASASKEAALF
                     IQDTFMNRSFRIYVNPDVVGVELGGALKNIIALAAGVCDGLKLGDNTKAALMTRGITE
                     MARLGVAMGGCAQTFAGLTGIGDLIVTCTSVHSRNHRAGVFIGEGIPAERAIEKVGMT
                     VEGYRTTRAAFQLSQKFQIEMPIVCECYRVLYESKNPRSAISDLMQRKKKHEIEEAWL
                     SKIKWE"
     gene            complement(6092..6745)
                     /gene="plsY"
                     /locus_tag="VHR42_07680"
     CDS             complement(6092..6745)
                     /gene="plsY"
                     /locus_tag="VHR42_07680"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX3027090.1"
                     /translation="MPDFTIILPFFLTAVAAYLIGSLSFSIIITRLLTNTDVREHGSG
                     NAGATNVLRTAGKGPALLTFALDFLKCVVSIILALYFFGLFTGVGVDMMFIRFTGGLF
                     CLLGHAFPLYFGFKGGKGVTTSAALVFMINWKCFLILLGVFFIVLLITKIVSLSSMIG
                     ISFFPAATLLIEYLDHAPAFYLAVNTFFALLIAGLVVLRHRKNIIRLKNGTESKIVQ"
     gene            complement(6817..>7026)
                     /locus_tag="VHR42_07685"
     CDS             complement(6817..>7026)
                     /locus_tag="VHR42_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX3027091.1"
                     /translation="PPSDKGKRLKILYMTQPSTKPPTFVVFVNKAELFHFSYQRYLEN
                     QIRATFGLEGTPIRFIVRERNDSRR"
BASE COUNT         1924 a         1785 c         1507 g         1810 t
ORIGIN      
        1 catgaaaagc tctcccccgt tcttgcttat catgtggtgg attccaccga agccggtttc
       61 gcagcggcgc tgagaatgct tgaaatcggc ggcatgggtc actccgcagt tatccactcg
      121 gaagatcaag cgttgatcgt aaaatacggc gacgccatga aggtcggacg cgtcattgcg
      181 aattccccct cctcccaggg cgccatcggc gatatctaca acaccaatac cccttccctg
      241 acgctgggct gcggctccta tggccacaac tccacaacct ccaatgtctc ttcggtcaac
      301 ctgatcaaca aaaagcgtct tgcaaagagg agggtcaaca tgcaatggtt taaggttccg
      361 ccgaaaatct attttgagcc caactcggtt cagtatcttg agaagatgcc cgatatttcc
      421 agagcgttta tcgtcaccga cccactgatg gttaaactcg gttatgtgga caagattctt
      481 ttctatctgc gccgccgcaa cgaatactgt cacagtgaga tcttctctga ggtcgagccc
      541 gatccgtcgg ttgatacgat catgagaggc gtcgaaacca tgaaccagtt taaacccgat
      601 gtcattatcg ctctcggcgg tggctccgca atggatgccg ccaagggaat gtggctcttc
      661 tatgagcagc ctcaaaatga tttcaatgcc ctgaggctta aatttatgga tatccgcaaa
      721 cgcgcctacc attttcctga gctgggcaaa aagtgccaga tggtcgccat cccgacaaca
      781 tccggaaccg gttcggaagt cacggcgttc gcggtcatca ccgacaagac caataatgtg
      841 aagtatccgc tcgcagacta cgcgctgact ccgaacgtgg ccatcgtcga cccgcagttt
      901 gttatgacaa tgcccaaaac gaccaccgcc gacacgggaa tggatgttct gacccacgcc
      961 attgaatcct atgtctcggt tctcgcttcc gattacaccg acggtctggc catgaaagcc
     1021 atcgaactgg tctttgacta tcttccccgc gcttataacg acgggagcga tcaggtcgcg
     1081 cgtgaaaaga tgcacaacgc ttcctgcatg gccggtatgg cgttcacgaa cgcattcctg
     1141 ggccttaacc actccatggc gcataagctt ggcggcgagt atcacattcc ccacggccgt
     1201 gcaaacgcca ttctgctccc ctttgtgatt gcctataacg gcagcaagcc gaccaaattc
     1261 acgagctggc caaaatacga atcctttgtc gcggatgaga aatacgcaca gatcgcccgt
     1321 catttgggcc tcccggcttc caccgccgcg gaaggcgtcc agtcacttgt caaagcggtg
     1381 caggatctgc tggttaagct gaatgtacca acctccatcc gcgcctgcgg cgtagacgaa
     1441 gccaccttct acaagaatgt cgaatatctt gccgatagag cgtttgaaga ccagtgcacc
     1501 acagcgaacc ccagactgcc gctggtcaaa gaaatcgttg aaatttacaa aaaagcttat
     1561 aacggaaact gatttaagcc cggataagac gttgccgccg gtttttagga accggcggct
     1621 tttatttgtt atcaaatttt tttaaaattc agatctttcc ctgaagttat ttaaaataac
     1681 tttcctttca gcttttcaag ggcgaacaga aaaggcagac ccagcaaata gcagacagct
     1741 gcttctccaa gtgcgacata cagcattgtg agaagaagcg gcagtttata aatcaggtga
     1801 agctcgacgc caacaaaaat cccgttcacg ataacgggcg gcagcggcgc cagcgcctta
     1861 ttaggcattt ttctggtaag ataggctgcg atcaaagaag ccgctccgcc gaccgccatg
     1921 tcgataggcc caagcgggct ggctatattc gcaagaacac agccgacaaa aagaccggga
     1981 atggccgcag gtgtaaaaaa cggcaggact gtcataattt ccgaaatccg gaattggatc
     2041 tcgcggaacg aaatcggcgc aaataccaat gtaagaacac aataaattgc agcgatcgcg
     2101 gccgccatag ctataaattt attcttttgg ttcattttca cttttagttc ccttcatttg
     2161 cacaaaatta aataaaaaaa taaactatac cggctttaaa aactgtgcgg acagaaaaaa
     2221 tccgcaaagt tttgaaacta ccgaaatagt tttcaggccc gtagttttgt ttatagacag
     2281 gatggttacg aactgtctta tttttttata ctttaacaca cgcagaataa aaagtcaatt
     2341 gtcactgtaa aaatagagaa gccaaatttt acttgaatta tcgtcggcta ttgtgtaaaa
     2401 tagagtattg aagtgatgga gaccgtcaga agaggaaaga agaagcaggt gttaaaatga
     2461 agctttcccg ccaggtcagc aaagggattt cgaccggtat attaatcctt gttttgattt
     2521 ttatctatat aaatttcaac accatattta ccaaattaaa ttataatgcg ctggcagtta
     2581 ccgacaacca aaaagatgaa acgattaacc tgcccaccca gatcaatttt ctcagcagcg
     2641 acaaaaaaga gtttgttgaa tactgcagcc ttacgtttga tgtaaacctt tccgaccaat
     2701 ccggaaacag ggtgtttacc tattacggcg caatcaacgg ctatcggttt tatcgcctgc
     2761 agctcaactg catcccctgc gacagcgcgt ttaccgaaga gaatattggc ggttatacgt
     2821 tcacgagccc ctgttattac agacccaacc cgaccggtct ttatattatc ggggacgatg
     2881 gcgtctacac acttaaagaa gcctacgaaa aaggcctgat caacatcagc aatgcctatt
     2941 cgctttatca aaagaaaaat atcacttaag atcctcaaaa aggacaaatg tgttttttcc
     3001 catctgtttg gcctgataca tggcgaagtc ggcccgccga ataatttccg acaccgaacg
     3061 tacggtaaag tcaaacattg cgataccgat acttgcggtt atcaggatcg ggctgttttc
     3121 cacttcgacc ggctttttga aatcttcaag cagcttttta gcgacatgga ccgcattttg
     3181 cttactgtgt attgagctga gcaaaaccaa gaattcatct ccgcccaatc ttgcgataaa
     3241 atcatctttt cttgtgttca gaagaagcct ttgggcaaat ttcttcagaa gctgatcccc
     3301 ggcgagatga ccgtaagtat cattaatgtt tttaaaccca tccaaatcca gaaacatcag
     3361 tgcgtatccg cccgcgatat cattgctttt gagaacgttg ccgataatct gcatcatcgc
     3421 atgccggctc aaaagaccgg taagcaaatc ggtctgggca atcttccgga tgtattcttc
     3481 cgactcctta atttccagat agcccttttt cagctcattg atcaacgaag tcagcttcat
     3541 ttcattattt acccggtcgg tgatatctct cacctgaagc atgacaaaaa catttccgtt
     3601 aatcgtgaca ggttcaacct gcatattctg gcgcacatca acgccgggat ttttttgggg
     3661 gtaaataaag gcatgatgaa tcaggcttga acaaaaacgt ttttggtttt gttcaagcgt
     3721 tttttttaga atatcctgat atcggtttcc tgcaaaaacc ggaaaaagct tcgcaaggtt
     3781 ttgccctaac gcctgctcat acagaattcc cgacaatttt tcaatatacc ggttgataaa
     3841 tcgtatcgag tgggaatcgt caatgatgat gattcctatg tcggcccttt caagggcttc
     3901 ccttaaaaga tcatacattc aattcaacca acatctcatc aattttgtct atgagcattt
     3961 tttctgaatt cattgtcaaa gctataaaaa ttactccttt tacctgactc tcggataata
     4021 aaaacgaggt atacatagat aaaaagtgca tttcttccgg aagaatcgtg ttttcaacat
     4081 cactgatcat cgtcatttca attgacggaa aggtgtactc cagtctaaca tcgagaaggt
     4141 ttccaaattc cccgatgacc gcattgaaga tgatgttgct gatttcttta ataacatcaa
     4201 gatcctcaag gctcaatttt tgaaaatctg cgtcctcgga ggcggagctt tcacccgtac
     4261 aggcattgat cagatttttg gccttatccg ccggaaaaac gatgtaagca ttgccggaaa
     4321 aacgatttcc aaaccgcagt gtcgacatca cggattttga aaaatccgcc atattcctgt
     4381 cgatatcgcg gaaatcaatt tcattgcctt ttttcaaatc aagttcaggt accgataaaa
     4441 taaccttttg actggccatc tcggaaagaa gactggcagc caaaccaata ttggtgttca
     4501 tgacttccac caagacgtct ttttccattt ccgaaagcat tttatacccc caagctgccg
     4561 ggcgggctac cgtccgataa attcatagat ttcctgaatg ttttcacgtt tgatgggttt
     4621 gtttaaaaat gccgacacgc caagacccaa tatctcatcc ttgacgcttt tttgaatatc
     4681 ggcggacaca acaatgactt tgatatcccg gtctgccagc cgaacctttt tgatgaattc
     4741 ctcgccgccc attccgggca tcagaaggtc ggtgataatg acatcaaaat gatcattcag
     4801 aaacttttga tatccttcct caccggaagc cgcaaacgta agttcgagcc cgggattgag
     4861 ctctaaaaga aatttccgga taaattgccg aacaaaaagc gaatcatctg ctatcagtat
     4921 cttcatcata ttcacctttt aaattagtcc tttttattgt aaattgcttt tcgccggttg
     4981 tcaaaacatt ttttccatta atgaaaaatt attcccattt tattttgctc agccacgctt
     5041 cttcaatttc atgtttcttt tttctctgca tcaggtctga aatcgccgaa cggggatttt
     5101 tcgactcata aagaacccgg taacattcgc atacgatcgg catttcaatt tgaaattttt
     5161 gtgacaattg aaatgcggcg cgcgtcgtcc ggtaaccttc caccgtcata cccacctttt
     5221 caatagcgcg ctcggcgggg attccttctc cgataaaaac gccggccctg tggttacggc
     5281 tgtgaacgct cgtacaggtc acaatcagat cgccgatccc cgtcaggcct gcgaacgtct
     5341 gggcgcagcc gcccattgcg acgccgagcc tggccatttc ggtaatcccc cttgtcatca
     5401 gggcggcttt ggtgttatcc cccagtttca gcccgtcaca gactccggcg gcaagcgcaa
     5461 taatattttt aagagcgccg ccaagttcca ccccgaccac atcgggattg acataaatgc
     5521 ggaacgaccg gttcataaac gtatcctgta taaaaagggc ggcttctttt gatgcggagg
     5581 cggccaccac cgtggtcgga acgccgcggg aaacctcttc cgcgtgcgat gggccggaga
     5641 gtattacgac ttttgcctgc ggcagctcct ctgcgattac ctgagacagc cggctgagcg
     5701 tttcttcttc aaatcccttt gcgacattga cgataacctg ccccgggcgt atataatctt
     5761 tcagtcgaac ggcagtctgc cggacggcaa aagatggaac agccaaaaca atcacatccg
     5821 ctccgttcgc taccacagcg tcggaagtca ggttgatttt ttccggaacc cgaactcccg
     5881 gcagcagctt tttatgctcc ccgtctctgc ggatgctttc caattcgttt tcaaagggag
     5941 accaaagact gactctgtgt ccgctttccg cgcacattac cgaaagggcc gttccccagc
     6001 ctcccgcgcc aaatacagcg atatttgcca tacccaacat cctttcagca aactggattc
     6061 aacttcacgt tattgtctga agctgacccg tttattggac aattttcgac tctgtgccat
     6121 tttttagcct gattatattt tttctgtgcc ttaaaacgac cagccccgca atcaacaggg
     6181 caaaaaacgt attcacagca agataaaacg ccggcgcatg gtcaagatac tcaatcagca
     6241 gcgtcgcggc cgggaaaaat gatattccga tcatggatga gagggaaacg attttcgtga
     6301 tcagcaggac aatgaaaaaa acgccgagca aaatcagaaa gcacttccag ttgatcataa
     6361 acaccagtgc cgcgctggtg gtaacgcctt ttcccccttt aaacccgaaa tacaacggga
     6421 acgcatggcc cagcagacaa aaaagccccc ctgtgaagcg gataaacatc atatcgacgc
     6481 ccactcccgt aaaaaggccg aaaaaataaa gcgccaatat aatggaaacg acacatttta
     6541 aaaagtccag cgcaaaagtc agcagcgcag gtcccttgcc ggctgtcctc aaaacattgg
     6601 tggctccggc gtttccgctt ccatgctctc ttacgtcggt atttgtcaga agcctggtta
     6661 taataatgga aaagctcagg ctgccgatca gataggccgc taccgcagta agaaaaaaag
     6721 gcagaatgat cgtgaaatcg ggcatgaaat atcctcctca accgggttaa tccgcgaccc
     6781 cgatatattt ttgggcacac ggattgcagg gacgcattat ctccgagaat cgttgcgttc
     6841 ccgcacaata aaccgtatag gcgtaccttc aagaccgaat gtagccctga tctggttttc
     6901 caaatatctt tgataggaaa agtggaaaag ctcagccttg tttacaaaca cgacgaaagt
     6961 cggcggtttg gtggaaggct gggtcatata tagaatttta agccgtttac ccttgtcgga
     7021 cggagg
//
LOCUS       DATALY010000279         4557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_236596,
            whole genome shotgun sequence.
ACCESSION   DATALY010000279 DATALY010000000
VERSION     DATALY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236596"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..680
                     /gene="def"
                     /locus_tag="VHR42_07690"
     CDS             207..680
                     /gene="def"
                     /locus_tag="VHR42_07690"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3027092.1"
                     /translation="MAIRNILNEKEPILHKMCRPVTEFNEHLWQLLDDMAETLHQADG
                     VGLAAPQVGVLRRMFVMDVGDGVVDVINPQYLVKKGKQENVEGCLSLPGIYGITRRPA
                     YVKIKAYDRYGKEFTIEGEDLLARCICHESDHLDGVIFTKHVIRMLEPENKDKEK"
     gene            738..1670
                     /gene="fmt"
                     /locus_tag="VHR42_07695"
     CDS             738..1670
                     /gene="fmt"
                     /locus_tag="VHR42_07695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX3027093.1"
                     /translation="MRILFMGTPEFAVPCFERLLSDGHEIVLTVTQADKPKGRGYVMT
                     PSPVKAAALSHGIPVYQPKTFKNNDEALKMLSDCRPDVAVVVAYGKILPQSVLDIPKH
                     GCVNVHASLLPKYRGSAPYQWAVLNGEKETGVTTMLLDAGIDTGDMLMKRHVTIPEDM
                     TAGELIDRLMAEGAEALSDTLKAMEAGELTPVKQDESLSSYAPMLSKKLSPIDWGHTA
                     EEIHNQIRGLNPWPSAATAFGGKLLKIHRSKTAGKSEACPGTIVKLRPFTVSCGGNTS
                     LEIQEVQLEGSKRMAAAEFLRGHGLKVGQILSDL"
     gene            1780..2478
                     /locus_tag="VHR42_07700"
     CDS             1780..2478
                     /locus_tag="VHR42_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX3027094.1"
                     /translation="MPFFFMDYWYIVLVVPALLISVWAQIKVKSAFSKYSKMGTRSGM
                     SGREACIAIQKYNGLSLPIETVRGSMTDHYDPRSNVIRLSETVGPVQSIAAIGVAAHE
                     TGHALQYANGYGPIKLRAAILPTTQFASTLSPYLVIAGLIFNMSYLAYVGIAFFGMAV
                     LFQLITLPVEFDASHRAMQALESEGILSEEELAGARKVLSAAAMTYVAALLVSLMSFI
                     RLLLIVNGRGRRGD"
     gene            2478..3818
                     /gene="rsmB"
                     /locus_tag="VHR42_07705"
     CDS             2478..3818
                     /gene="rsmB"
                     /locus_tag="VHR42_07705"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX3027095.1"
                     /translation="MAENARQAAVEALIRVHREGSYSNIALDSLLNSSRFSAADRALL
                     SRLFYGVIERQLTLDYILSNYSRIKLKKMHPTVLELLRVGCYQLVYMDKIPASAAVNE
                     SVRLARVMKQERSSGFINAVLRSVERDRGHWFDRLEENTDGLSIRTSCPVWLLSLWQD
                     SYGASMAKKIAESVNEAPPLTLRINTLKITSDEFYHKLEQANIAFQKIPGLEACATVT
                     DTGALKGLAETMENCYYHQDAASQFCCRALEPKRGERIADVCAAPGGKSLTAAQMMEN
                     QGEIFACDIYPAKCETIEKRARQMGASIVHTAVRDASSPCPEPLKGRFDRVLCDAPCS
                     GLGAIRRKPEIRYKQPASFSELPLLQSEILNRSAQLVRMGGVLQYSTCTLNPAENEEV
                     AKKFLEEHPEFEPRRLPLEECFAALNTEPTYQITLLPPVHHTDGFYISSFIKTR"
     gene            3823..>4557
                     /gene="rlmN"
                     /locus_tag="VHR42_07710"
     CDS             3823..>4557
                     /gene="rlmN"
                     /locus_tag="VHR42_07710"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX3027096.1"
                     /translation="MNLLDCKSMSLEELREQLIQRKIPAFRAVQIKTWLDNGTADFDK
                     MLNLPLTLRQNLKNIFTIPTVNIEKKLVSSRDNTVKYLYRLNDGENVESVLMEYQHGW
                     SQCLSTQVGCQMGCSFCATGMGGFVRNLQPSEMVAQIEKAQQDHNIRVSSVVLMGMGE
                     PLNNFDNVLRFLNMLAEPGGVHIGMRHISLSTCGLVDKIYKLMEYKLQITLSISLHAP
                     NNQIRSRLMPVNRKWPIEELLEACRKY"
BASE COUNT         1193 a          999 c         1209 g         1156 t
ORIGIN      
        1 tgcaactgtc ggacgtgtgg cgggaaaatt ccggtataaa ttattaatga aaacgattaa
       61 taatagtaag atgagggaaa tgaccgcacg gctgctgacg gaattcagcc gcgcttccga
      121 aaataaatcc gtgacggttt acgtggacat taatcctgcg aatatgctgt aaagcatatc
      181 gattctattg gaaagggcga aataatatgg ctatacgcaa tattttgaat gaaaaagaac
      241 ccatactgca taaaatgtgc cgtccggtta ccgagtttaa cgagcacctg tggcagctgc
      301 ttgacgatat ggcggaaacc ctgcatcagg cggacggcgt cggattggct gcgccgcagg
      361 tcggcgtgct tcgccgtatg tttgtgatgg atgtcggcga cggcgttgtc gatgtaatta
      421 atcctcagta tcttgtgaaa aagggaaagc aggaaaatgt cgaaggctgt ctgtctctgc
      481 ctggaattta cgggataacg cggcgtcctg cctatgtgaa aataaaagca tatgaccgct
      541 acggcaaaga attcacgatc gaaggcgagg atctgctcgc ccgttgtatt tgccatgaaa
      601 gcgatcacct tgacggtgtt atctttacaa agcatgtgat tcgtatgcta gaaccggaaa
      661 ataaggataa agagaaataa tcgcagatcc tgtaaataag ctgacgactg aaattgtcgg
      721 aaaggtttga tatttcattg agaattctgt ttatgggtac tccggaattt gccgtacctt
      781 gttttgagcg gctgctttcg gacgggcatg aaatagtttt aacggtaaca caggccgata
      841 aaccgaaagg ccgcggatac gtcatgacgc cctctcctgt aaaggcagcg gctttgtcac
      901 atggaatacc ggtctatcag ccgaaaacgt tcaagaataa tgatgaagct ttaaagatgc
      961 tgtccgattg cagaccggac gtcgcggttg tcgtcgctta cggtaagatt ctcccgcaat
     1021 cggttttgga tattccaaaa catggctgcg tcaacgttca cgcatccctg ctgccgaaat
     1081 atcgcgggtc ggcgccgtat cagtgggcgg ttctgaacgg tgaaaaggaa accggagtta
     1141 ccacgatgct tttggacgcc ggaattgaca ccggcgatat gctgatgaag cgtcatgtca
     1201 ctattccgga ggatatgaca gcgggagagc tcattgaccg cctcatggcg gaaggagcgg
     1261 aagcgttatc cgatacgctg aaagcaatgg aagcgggaga actgacgccg gtaaaacagg
     1321 atgaatctct gtcaagttac gcacctatgc tgagcaaaaa actcagccct atcgactggg
     1381 gccatactgc agaggagata cacaatcaaa tacgcggtct gaatccgtgg ccctcggcag
     1441 caacggcatt cggcggaaaa ctgctgaaaa tccatcgctc taaaacagcg ggaaaatcgg
     1501 aagcgtgccc gggaacgatt gtaaagctaa gaccatttac agtttcctgc ggcggaaata
     1561 cgtcgcttga aattcaggaa gtgcagcttg aagggtctaa gagaatggct gctgccgaat
     1621 tcttacgggg acatgggctg aaagtcggtc aaattttgtc ggatttatga tattatcatg
     1681 aatctgtaaa ttattcataa gatattcatt tttccgggct atactaaaat taatgggtta
     1741 gcaataaaag atgtctaaat taatgagagg tagagtccta tgcccttctt ttttatggat
     1801 tactggtata ttgttcttgt ggttccagcc ctgctgattt cggtctgggc gcagatcaaa
     1861 gtgaaatccg cttttagcaa atacagtaaa atgggaacga gaagcggtat gtcgggcagg
     1921 gaagcctgca ttgcgattca aaaatataac ggactgtctc tgccgattga aaccgtccgc
     1981 ggctccatga ccgaccacta tgacccgcgc agcaatgtga ttcgcctgtc cgagacggtg
     2041 ggaccggttc agtcgattgc ggcgatcgga gtggcggcgc atgaaaccgg ccacgcactg
     2101 cagtatgcga acggttatgg ccccattaag ctgcgcgcgg caattttacc gacgacgcag
     2161 tttgcttcca ctttatctcc ttatttagtg atcgcgggtt tgatttttaa tatgagctat
     2221 cttgcttatg tcggcattgc ctttttcggc atggcggtgc tgtttcagtt aatcacattg
     2281 ccggtcgagt tcgatgcgag ccatcgggcg atgcaggcgc ttgaatccga aggtatttta
     2341 tccgaagagg aactggcggg tgccagaaaa gtgttgtccg ccgccgcaat gacgtatgtt
     2401 gccgcgctgc ttgtttccct gatgagtttt attcgtttgt tactgattgt caacggccgc
     2461 ggcaggaggg gcgattaatg gctgaaaatg cccgccaggc ggctgttgaa gcgctgattc
     2521 gcgtacatcg cgaggggagt tattcgaata ttgcgctgga ttctctgctg aacagttcaa
     2581 ggttcagtgc ggcagacaga gcgctgctgt cccgcttatt ttacggagtt atcgaacgtc
     2641 agctgacgct cgattatatt ctttcaaatt attcccgcat aaagctgaaa aagatgcacc
     2701 cgacagtatt ggaattgctt cgagtcggat gctatcagct tgtctatatg gacaagatcc
     2761 cggcttccgc cgccgtgaac gaatcggtca ggctggcgcg ggtcatgaag caggaaaggt
     2821 cctccggttt tatcaatgcg gttctgcggt cggtcgaacg tgaccgcggc cattggtttg
     2881 accgtttgga ggaaaatacg gacgggctgt cgatacggac atcctgtcct gtctggctgc
     2941 tctctctgtg gcaggattct tacggtgcgt ccatggcaaa gaaaattgcg gaaagtgtca
     3001 atgaagcacc gcccttaacc ttacggatta atacattgaa aataacgtcc gacgagtttt
     3061 atcataaact tgaacaggca aatatagcct ttcaaaagat tcccggtctg gaggcctgcg
     3121 caacggttac cgataccggt gcattaaaag ggcttgcaga aaccatggaa aattgctatt
     3181 atcatcagga tgccgcttct cagttctgct gcagagcgct ggaaccgaaa cggggggaac
     3241 ggattgccga tgtttgcgcg gctccgggcg gaaaaagcct gacggcagcg cagatgatgg
     3301 agaatcaggg cgagatattt gcctgtgata tttatccggc aaaatgcgaa accattgaaa
     3361 aacgggcgcg ccagatgggc gcttcgatag tgcatacggc tgttcgggat gcttcgtcgc
     3421 cgtgtccgga gccgctgaaa ggccggtttg accgtgtttt atgtgatgcg ccctgttccg
     3481 gactgggagc cattcggaga aagccggaga ttcgctataa gcagcccgca tcattttcag
     3541 aacttccgct gctgcagtct gaaattttaa acagatcggc gcagctggtt cgtatgggag
     3601 gagtgctgca gtattccact tgtacgttaa accccgctga aaatgaagag gtagcgaaaa
     3661 agtttttaga agaacatccc gagtttgaac ctcgccggct tccgcttgaa gaatgttttg
     3721 ccgcccttaa tacggaaccg acctatcaga tcacgttact tccgccggtt caccataccg
     3781 acggtttcta tatctccagc tttattaaaa cgaggtgact ttttgaatct tcttgactgt
     3841 aaatccatgt cgctcgaaga attgcgggag cagctgattc aaagaaaaat acctgcgttc
     3901 cgcgcagtgc aaataaaaac atggttggac aacggaacag cagatttcga caaaatgttg
     3961 aatcttccgc tgaccctgcg gcagaatttg aaaaatattt ttacaattcc tactgtaaat
     4021 atagaaaaaa agcttgtatc ttccagggat aatactgtaa aatacttata tagattgaat
     4081 gacggcgaaa atgttgaatc cgttttgatg gaatatcagc atggatggtc gcaatgtctg
     4141 tcaacgcagg tcggctgtca gatgggctgt tcgttttgtg ccacaggcat gggaggattt
     4201 gtccgcaatt tacagccgtc ggaaatggtt gcgcaaatag aaaaagcgca gcaggatcac
     4261 aatattcgcg tatcttcggt cgttttaatg ggaatgggag agccgctgaa taattttgac
     4321 aacgtactcc gctttttaaa catgctggcg gagcccggcg gcgtgcatat cggaatgcgt
     4381 catatctcat tatctacctg cggattagtg gacaaaatct ataaattgat ggaatataag
     4441 ctccagatca ctttatcgat ctctcttcac gcaccgaata atcagattcg ttcacggctg
     4501 atgccggtga atcgaaaatg gcccatcgaa gaactgctcg aagcctgccg taaatat
//
LOCUS       DATALY010000280         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_577023,
            whole genome shotgun sequence.
ACCESSION   DATALY010000280 DATALY010000000
VERSION     DATALY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577023"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..1708
                     /gene="pepT"
                     /locus_tag="VHR42_07715"
     CDS             491..1708
                     /gene="pepT"
                     /locus_tag="VHR42_07715"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX3027097.1"
                     /translation="MRAYERLLKYIKWDTTSDEDAPEGICPSTPGQKDFALSLAAEMK
                     SLGLSGVHLDSHGYVYGTLEANCETDSPTLGLIAHMDTSPSAKGADIQSRIVQNYDGK
                     DILLNRENSIVMHASDYESLSDYVGQDLIVTDGTTLLGADDKSGIAEILTAVENIISQ
                     EVKHGTVQIAFTPDEEIGRGADLFVVQGFGADFAYTVDGGKIGELEYENFNAASAEIR
                     ISGVNIHPGDAKNKMKNALLIGMEFNRLLPPHEIPACTEGYEGFYHLTNFSGNEESAV
                     MHYIIRDHDRRKFECRKERMQKNADYLNEIYGTKTVSLQLRDSYYNMKEKVEPHLFLI
                     ENAKKAMSQAGVTPRVLPIRGGTDGARLSFMGLPCPNLSTGGHNFHGRFEYISVQAMD
                     KMTEVLINLVRVE"
BASE COUNT          489 a          438 c          423 g          421 t
ORIGIN      
        1 ttgaatcact cttgcagtag ctgcagatca ttgatattac ctccattata aaagccctga
       61 attcatcagg gtttgaaaaa ttgactattt tttacaaata ttctctaaaa tcagcaaaac
      121 ttttcttgta tttttactca aatctacaat taaagcatat tatatcactt tatcagagaa
      181 aagggaatat tcccaggcat ctaaaaataa tgccggattt tttaacgctc cgcaatgttc
      241 agagatgttc gggaacggcc tgtgtcggaa agtatctact atatgaaacc cggccaggcg
      301 gttctctgtt ttccaattta ttgatttgtg acgaaatgcg acttataata agcataataa
      361 gaaatgtacg gggcagaaac tctgctcccc cgcagctgca aatcagccat actccgacgg
      421 acaagcccgg cagtccattc aggccggcgg aatacggcat aatattatta ttggagggga
      481 atttatcatc atgagagctt atgaacgcct tctgaaatac attaaatggg acaccacgtc
      541 agacgaagac gcacccgagg gcatctgccc gagcactccg ggccagaaag actttgccct
      601 gtctctggca gcagaaatga aatcgcttgg attgtcgggg gtccatctgg actcccacgg
      661 ctatgtctat ggcacgctgg aggcaaactg cgaaaccgat tcgccgacgc tcggcctgat
      721 cgctcatatg gatacaagcc cctccgcaaa aggtgctgat atccagtccc gcattgtgca
      781 gaattatgac ggaaaagata ttctcctgaa ccgggaaaat agcatcgtca tgcacgcatc
      841 ggattacgaa agcctgtcgg attatgtcgg gcaggatctc attgtcaccg acgggaccac
      901 ccttttgggc gccgacgata aatccggcat tgcggaaatc ctcactgcgg tggaaaatat
      961 catcagccag gaggtaaaac acggaacggt tcaaattgct tttaccccgg atgaagaaat
     1021 cggccggggc gcagatcttt ttgtcgttca gggatttgga gcggattttg cctataccgt
     1081 cgacgggggg aaaatcggcg aactcgaata tgaaaatttc aacgccgcgt ccgcggaaat
     1141 cagaatcagc ggggtgaaca ttcatccggg cgatgcaaaa aacaaaatga agaatgccct
     1201 tctgatcggg atggagttca accgcctgct cccgcctcat gaaatcccgg catgcaccga
     1261 aggctatgaa ggtttttatc acctcaccaa tttctccggc aacgaggaaa gcgccgtgat
     1321 gcactatatt atccgcgacc atgaccgccg taaatttgaa tgccgaaaag agcggatgca
     1381 aaaaaacgcc gattacctga atgaaattta tgggacaaag accgtttcgc tgcagctgcg
     1441 ggattcctat tacaacatga aggaaaaagt tgaaccccat ctgtttttga ttgaaaacgc
     1501 taaaaaggca atgtctcagg cgggcgtcac ccccagggtc ctgcccatcc gcggcggaac
     1561 cgacggagcg cggctgtctt ttatggggct tccctgccca aatctttcga ccggcggtca
     1621 caacttccac ggccgctttg agtatatttc ggtgcaggcg atggataaga tgaccgaagt
     1681 gctgatcaat ctggtcaggg tcgaataacc ccctgcttcc ggtctttcaa gatggacggg
     1741 gaactccttc cccccccgct gtcataagat a
//
LOCUS       DATALY010000281         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_322036,
            whole genome shotgun sequence.
ACCESSION   DATALY010000281 DATALY010000000
VERSION     DATALY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322036"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VHR42_07720"
     CDS             <1..1161
                     /locus_tag="VHR42_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3027098.1"
                     /translation="LLSGGVDSSYVASYFSGEKTFTVGFEGGAKYSEIGYAQELSEKI
                     GTEHHRHIISPEEYWGNFRKVQYFMDQPLADASCVALYFVMKMARQYVKVVLSGEGSD
                     ELFGGYNIYHEPDDLASYQRLPRGLRHAFGALAEKMPAVRGRSFLIRGSKTLEERFIG
                     NCSMFSMAEKKRLLKDSIPATVPQLVTKPVYERAQGLDDVTKMQYLDINVWLPGDILL
                     KADRMSMANSVEVRVPFLDRRVFDVASRLPRRLKVNAKNTKYALREAALRHLPEETAQ
                     RKKLGFPVPIRVWLREEKYYGIVKSAFTSETAEQFFNTDVLVDMLDAHRSGKCDNSRK
                     IWTIFAFLVWYDVYFNGVSHEEGDMPDFFSSGEAKRTETVASEEPEKVPVNL"
     gene            complement(1334..>1521)
                     /locus_tag="VHR42_07725"
     CDS             complement(1334..>1521)
                     /locus_tag="VHR42_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3027099.1"
                     /translation="SWLCYYRALQEGPASVVVPIDQLSIVLTVAFSYFIFKEKVTKKS
                     IAGLSLLVAGTMALLIH"
BASE COUNT          384 a          367 c          404 g          366 t
ORIGIN      
        1 cttctctcgg gcggtgtgga ttccagttat gttgcaagct acttttcagg tgaaaaaaca
       61 tttacggtcg gctttgaagg cggcgcaaaa tacagcgaaa tcggctatgc tcaggagctt
      121 tccgagaaaa tcggaacgga acatcaccgc catatcattt ctccggagga gtactgggga
      181 aatttccgca aagttcagta ttttatggat cagccccttg cagacgcatc ctgcgttgca
      241 ctttattttg tcatgaaaat ggcccgccaa tatgtgaagg ttgttctttc cggggaagga
      301 tccgatgaac tgttcggcgg ctataacatt taccatgagc cggatgacct cgcatcctac
      361 cagcggctgc cgcgcggcct gcgccatgcc tttggggcac ttgcggaaaa aatgccggcg
      421 gtcaggggaa gaagtttcct catccgcggt tcgaaaacgt tggaagagcg ttttatcggt
      481 aactgcagta tgttctcaat ggcggaaaag aagcgtctgc tgaaagacag cattccagcc
      541 acggtgcctc agcttgtaac aaagccggta tatgaacgtg cgcagggcct tgacgacgta
      601 acaaaaatgc agtatctcga tattaatgtc tggctgccgg gtgatatcct gctgaaggct
      661 gaccgcatga gtatggcaaa ttcggtggag gttcgtgtcc cgttccttga ccggcgtgtt
      721 tttgatgtgg cttcccgttt gccgcgccgc ctgaaggtga atgcaaagaa cacgaagtat
      781 gcgcttcgtg aggccgcatt gcgccacctg ccggaggaaa cggcacagcg caaaaagctt
      841 gggttccctg taccgattcg tgtgtggctg cgggaagaaa agtactacgg aatcgtaaaa
      901 tcagccttta cctccgaaac ggcagagcag tttttcaaca ccgatgttct ggtagatatg
      961 ctcgacgccc accggagcgg gaagtgcgac aacagccgta aaatctggac aatttttgct
     1021 ttccttgtct ggtatgatgt ttatttcaac ggtgtctccc atgaagaagg agatatgccg
     1081 gatttctttt cttccggtga agcaaagcgg actgagacgg tcgccagcga agaacccgaa
     1141 aaggttccgg taaatctgta ataaccgaat atcgtgcaga aatgccacgg aagaaagcgg
     1201 aagcacaata cgtgctcccg cttttctgcg cttccgatac ccacttccat tttataagcg
     1261 gacagtgatg ccgcccaaaa gtaaatccgg cttccgtatg cagcgaaaac cggattttta
     1321 tgtatttagg atgttaatga atcaacagcg ccatcgtgcc agcgacaagc agcgagagcc
     1381 cggcaatcga tttcttcgta accttttctt tgaatatgaa atatgagaat gctactgtca
     1441 gcacaatgct gagctgatcg atcggaacaa cgacgctggc cggcccttcc tgcaatgccc
     1501 ggtagtagca gagccaagaa g
//
LOCUS       DATALY010000282         4258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_836505,
            whole genome shotgun sequence.
ACCESSION   DATALY010000282 DATALY010000000
VERSION     DATALY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836505"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VHR42_07730"
     CDS             complement(<1..311)
                     /locus_tag="VHR42_07730"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX3027100.1"
                     /translation="MLNANPSEKFLKEGLTFDDVLLIPSKSDILPKQIDIKTKLTKKI
                     VLNIPLMSAAMDTVTESKMAIAIARVGGIGVIHKNMSIERQAEEVDKVKRSENGVIVN
                     P"
     gene            645..1085
                     /gene="spoVAC"
                     /locus_tag="VHR42_07735"
     CDS             645..1085
                     /gene="spoVAC"
                     /locus_tag="VHR42_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HEX3027101.1"
                     /translation="MITKKEYEQMTKESSPDSPLWENMLWAFFTGGAICTIGQGILML
                     YQSYGLNQLDASTSTSITLIFLGALLTGLKIYDNLAKHAGAGTLVPITGFANSIVSPA
                     LEFKSEGYILGMSTKMFSIAGPVLVFGITASVIYGLVIWIFRLF"
     gene            1108..2127
                     /gene="spoVAD"
                     /locus_tag="VHR42_07740"
     CDS             1108..2127
                     /gene="spoVAD"
                     /locus_tag="VHR42_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HEX3027102.1"
                     /translation="MLAKRIGRYTICFDQPVAVKGFASVVGKKEGEGPLAEHFDYISE
                     DTTFGEKTWEKSESRMQKDTLTRALNKAAVTPDTVDIIFAGDLLNQCIGTNYGLRDFG
                     IPFFGLYGACSTMAESLSLASVFVDSGSVNIAAALTSSHFCSAERQYRFPLEYGGQRT
                     PNSQWTVTGSGSVILSRDGDGPFIKEITVGKITDLGIKDANNMGAAMAPAAAETIEAY
                     FKDTSQKPSDFDLIVTGDLGLVGHRLLIELLEKDGIQVNPDKFNDCGMMIFDLENQDV
                     HAGGSGCGCSASVLCSYILNSMRCGKLHEVLFTATGALMSPMSTQQGESIPSIAHLIH
                     FSTKK"
     gene            2177..2533
                     /gene="spoVAE"
                     /locus_tag="VHR42_07745"
     CDS             2177..2533
                     /gene="spoVAE"
                     /locus_tag="VHR42_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HEX3027103.1"
                     /translation="MIFVKAFIIGGLLCLIGQILIDKTKMTPARILVTFVTMGVILTA
                     LGLYEPLVKWGGAGATVPLSGFGYSLAKGVMTGVREHGLVGVFTGGVTGTAGGIAAAI
                     FFGYLIALIFKPGDKS"
     gene            complement(2553..2828)
                     /locus_tag="VHR42_07750"
     CDS             complement(2553..2828)
                     /locus_tag="VHR42_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027104.1"
                     /translation="MQELKSKKIYPKDAGREKRLNELEREYDSKRDPVNDLRLEELNF
                     YLSGLLGRNNLHYLREYADLLIARYNTDAGWFYNKGYEDARRAGCPK"
     gene            complement(3331..4113)
                     /locus_tag="VHR42_07755"
     CDS             complement(3331..4113)
                     /locus_tag="VHR42_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX3027105.1"
                     /translation="MNINKSATSASESTPEYSMVSPGNYDTDPDQISTLVNQLASVTA
                     SEAVSLDMSDANLSKYGLKNPANTFSCTFNGKVYTLLFGNTETDSGTENIYVMLQGRN
                     IIYKIATSSLSFYNWNASNLVSRLLFLDNIDTVKTLTVTIDSKSYQFNLSGTDDKLKV
                     IYNSKTLNTSYFRNYYEDVLSNQVEDMATKPANGKVVCTVKVDYRSSSKASTTMQFTQ
                     IDDRRLFWQINGKGNFYILRTTVDKLISDTKLIAANKAVATS"
BASE COUNT         1037 a         1029 c         1045 g         1147 t
ORIGIN      
        1 aacgggttca caataacgcc gttttcactt cttttgacct tgtcgacctc ttcggcctgc
       61 cgctcaatgc tcatattttt gtgaataacg cctatgccgc ccacacgcgc aatggcaatc
      121 gccattttcg attccgtcac cgtatccatt gcggcggaca ttaacggaat gttgagcact
      181 attttctttg tgagttttgt tttaatgtca atctgcttgg gtaagatgtc tgattttgac
      241 ggaatgagca gcacatcgtc aaaagtcaga ccctctttca aaaatttttc ggatggattt
      301 gcattcagca tagttaccat tctcctttct cccgggtcgc cccgactctg caaccctgca
      361 taaaactcat cgaaagctca ggttccggct caaaatagta tgaaaaaaac ctgctgtaca
      421 tttctcaggc aatgtcgcag gatcccgtac aaattatgaa ttattttatt cattttaatc
      481 taattgaaac gctttgtcaa ggcggtcaaa ccttctgcgc cgaattctta cagactccat
      541 aacggatcgc taaatattaa cagccaatct attgaatatg ccttaccgtg tcggaaaaac
      601 taaattaaaa ccgcgcagaa ccgcggcggt cggaaggtga ttttatgata acaaaaaaag
      661 aatacgaaca aatgacgaag gaatcctccc ctgattctcc gctctgggaa aacatgctct
      721 gggctttttt caccggcggc gcgatctgca ccatcggtca ggggattctg atgctctatc
      781 agtcatatgg gcttaaccag cttgacgctt ccacatccac atccatcact ctgatctttc
      841 tgggagcttt gctgacgggg ctgaaaatat atgacaatct ggccaaacac gcgggagcgg
      901 gtactctggt tcctatcacc gggtttgcca attccattgt ttcacccgcg ctggaattta
      961 aaagcgaggg ttatattctt ggaatgagca caaaaatgtt ttccattgcc gggcctgttt
     1021 tggttttcgg tattacggct tccgtcattt acgggctggt aatctggatt ttccggcttt
     1081 tctgatacgc aagcataggg aggaattatt ttggcaaaaa ggataggaag atatacgatt
     1141 tgttttgatc agcctgttgc cgtcaaagga tttgcctcgg ttgtcggcaa aaaagaaggc
     1201 gaaggcccac tggccgaaca ttttgactat atatcggagg acaccacttt cggggaaaaa
     1261 acatgggaga aatctgagag ccgcatgcag aaggataccc tgaccagggc gctgaacaag
     1321 gcggccgtca caccagacac ggtggatatt atctttgcgg gcgatctctt aaaccagtgc
     1381 atcggaacaa attacgggct gagagacttc ggtattccgt ttttcgggct ttacggagcc
     1441 tgttcaacca tggccgagtc cctgtcgctc gcctctgttt ttgttgacag cggaagcgta
     1501 aacatcgcgg cagctctgac ctcatcccac ttttgctcgg ctgagcgtca ataccgtttc
     1561 ccgctggaat atggcgggca gcgcacgccc aattcccagt ggacggttac cgggtcagga
     1621 tcggtgattc tctcccgtga cggcgacgga ccctttatca aagaaattac cgttggtaaa
     1681 atcaccgatc tcggtattaa ggacgccaac aacatgggcg ccgcgatggc cccggcagcg
     1741 gcggaaacca ttgaggccta ttttaaggat acttcacaaa aacccagcga ctttgatctg
     1801 attgtgaccg gtgatcttgg cctggttggt caccgcctgc tgatcgagct gcttgaaaaa
     1861 gacggcattc aagtcaaccc cgataagttt aacgattgcg gaatgatgat tttcgatctc
     1921 gagaaccagg atgttcatgc cggaggttcg ggatgcggct gctccgcctc ggtactctgc
     1981 tcctacattc tcaacagcat gagatgcgga aagctgcacg aagtgctgtt taccgcgacg
     2041 ggtgcgctca tgagcccgat gagtacacag cagggggagt cgatcccctc catcgcgcat
     2101 ctcattcatt tctcaacgaa aaagtaatcc ggcacacttg cgatgctgta tgttgattgg
     2161 aggaggaaag ggtaacatga ttttcgtcaa ggcatttatc atcggcggcc tgctctgtct
     2221 aatcgggcag atcctgatcg ataagaccaa aatgacacca gcaagaattc tggtcacttt
     2281 cgtgacgatg ggtgtaattt taacggctct cggactttat gagccacttg taaaatgggg
     2341 gggagcaggc gccaccgtcc ctctgagcgg atttggttat tctctcgcaa aaggcgtgat
     2401 gacgggagtt cgggagcacg gtctggtggg ggtctttacc ggaggtgtta ccggaacggc
     2461 cggcggcatc gccgccgcaa tatttttcgg ctatttgatc gcgctaattt ttaaacccgg
     2521 agataaatcc tgaatcgtcc gggtttcccc ggctatttcg ggcaacccgc gcggcgggca
     2581 tcctcatagc ctttgttata aaaccacccg gcgtcggtgt tgtaacgtgc gatcagcaga
     2641 tcggcgtatt cccgcagata atggaggttg ttccgcccca acagaccgga taagtaaaaa
     2701 ttcagctctt caagccttaa gtcgtttacc ggatcgcgtt ttgaatcgta ttcgcgttcc
     2761 agctcgttca gccgcttctc acggccggcg tctttcggat agattttttt gctttttaat
     2821 tcctgcattt ctctcgctcc tctgttgata taacggattc gggcccgaaa aagcgggcgc
     2881 aggtattaca tacctacacc cgctcgacat acaaacccaa aaccgccaga ccaacagaca
     2941 agtcaaagaa gagggaagcg ctgagcgcac ctcgctttgc ttgcaatggg acggtgaatc
     3001 gtttataatt aataacgtcg gagcgccagc gaaagctggt tatgtaggtg tctattccac
     3061 ctgcataggc ctgccgtgtt ccaccacgtc aggttgcttc ggcccttttt ttcgggccgt
     3121 tttccgttaa gcctattata aaccagtaaa accggttatg caagatataa tttcctgtga
     3181 ttggcaaaaa ggcgaaatca aaaaagtttt atgctcatgg ttcccgtgtc ctacgaccgt
     3241 taagcggaaa aaaagagagg tatgccgaaa tggcatacct ctcttttttt attagaattc
     3301 ttgaactgct cagccgggca gctttccgtc ttatgaggtg gcaaccgctt tgttggcggc
     3361 gatcagtttc gtgtcgctga tcagcttatc aactgtcgtg cggagaatat agaagtttcc
     3421 cttgccgtta atctgccaga agagacggcg gtcgtcgatc tgggtaaact gcatcgtcgt
     3481 cgacgccttg gaggaggaac ggtaatccac tttgaccgtg cagacgactt tcccgttggc
     3541 cggtttggtc gccatatcct ccacctggtt gctgagtaca tcctcatagt agtttctgaa
     3601 ataggacgta ttgagggttt tggagttgta aattactttt aatttgtcat ccgttcccga
     3661 gaggttaaat tgataactct tggaatcaat cgttaccgtc agggttttta cagtgtcgat
     3721 attgtcgagg aacaaaagtc tcgacaccag attacttgca ttccagttat aaaacgacag
     3781 cgaggaagtt gcaatcttgt aaataatgtt tctgccctga agcatcacat agatattttc
     3841 ggttccgctg tcggtttccg tattgccaaa cagaagggta tatactttgc cgttaaaagt
     3901 acagctgaat gtgttggcag gattcttaag cccatatttg gaaagattcg catccgacat
     3961 atcaaggctt accgcttcgc ttgcggtaac tgaagccagt tggttgacaa gcgtggaaat
     4021 ctgatcggga tctgtgtcat aattgcccgg ggaaaccatg ctgtattccg gcgtggactc
     4081 agacgcggat gtcgcgctct tgttaatatt catcacaaca gccggcgatc tggaagttcc
     4141 cccccagcgt cattgaggtc agcttgaaca ggctgctgga gtccactgtg agaagactta
     4201 actcagcgaa ctgaatcggt ttggattcat agtagctgaa aaagtcactg tccgacaa
//
LOCUS       DATALY010000283         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_322535,
            whole genome shotgun sequence.
ACCESSION   DATALY010000283 DATALY010000000
VERSION     DATALY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322535"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..379
                     /locus_tag="VHR42_07760"
     CDS             89..379
                     /locus_tag="VHR42_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027106.1"
                     /translation="MIRVFENYVVTADENNFILGELKTRKGKDGQPVEYVSNISYHSN
                     LTGALDNCIKISQRRYIRDHDISLPESIKAIDEINQHLEKILAQSIKEHLSV"
     gene            479..925
                     /locus_tag="VHR42_07765"
     CDS             479..925
                     /locus_tag="VHR42_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEX3027107.1"
                     /translation="MIIKFTIPGSPMGKGRPKFARRGKFTATYTPEKTANYETLVKWY
                     FQQAAQGQNLKGALKADIFAYFPITKTDAKKKNLFSKMISGIIRPTKKPDWDNIGKIV
                     CDALNKMAYDDDSSIVDGRVRKYYSDQPKVEVTISEIVNFPEVEDA"
     gene            918..1388
                     /locus_tag="VHR42_07770"
     CDS             918..1388
                     /locus_tag="VHR42_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3027108.1"
                     /translation="MLNVAVLMGRLTADPELRHTSSDIPVTSFTMAVDRGYVKAGAER
                     QTDFIDIVCWRGTAEFVSRYFHKGMMIAVDGSIQTRSYTDKEGNKRKAFEIVAREVHF
                     AEPKRDSDRAASPQASGNVSSGSTQNTQNQAASSAPPQDNGDFVEIDGDDDLPF"
     gene            1394..2766
                     /locus_tag="VHR42_07775"
                     /pseudo
     CDS             1394..2766
                     /locus_tag="VHR42_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
BASE COUNT          865 a          655 c          727 g          640 t
ORIGIN      
        1 ctttcggatg gaatgaaaat tgataacccc aaaaaacaga taagcgactg gaaagacaaa
       61 tccacaaaaa tgatcggagg ataaaacctt gattcgtgtc tttgaaaatt acgttgttac
      121 ggcggacgaa aacaatttta tcctgggcga attgaaaacc agaaaaggca aggacggtca
      181 gccggtcgaa tatgtttcca acatcagtta tcatagcaac ctgaccgggg cgctcgacaa
      241 ctgcataaaa atatcccagc ggagatatat ccgggatcac gatatcagtc tcccggaatc
      301 cataaaagct attgacgaaa tcaatcagca ccttgaaaag attctggccc aaagcattaa
      361 agaacattta tctgtttgag cctgtacgga gtttgtagag cgaatttcaa ggttgacggg
      421 ttaaattaca tggttagatt atttgaagcg cagaaaaggc gcaaagggag gactgaacat
      481 gattatcaaa tttacgatac cgggatcacc gatgggaaaa ggaagaccga agtttgcaag
      541 gcgaggaaaa tttacggcta catacactcc tgaaaagaca gcaaattacg aaacgcttgt
      601 gaaatggtat tttcagcagg cagcacaagg tcaaaatctt aaaggcgctt tgaaagccga
      661 tatattcgca tactttccaa taacaaaaac agacgcaaaa aagaaaaatc tattttcaaa
      721 gatgatttcc gggattatta ggccaacgaa gaaaccggac tgggacaaca tcggaaaaat
      781 tgtttgcgat gccctaaata agatggcata cgatgatgat tcaagtattg tggatgggcg
      841 tgtgcgaaag tattacagcg atcagccgaa agtcgaagtc acaataagcg aaatagtgaa
      901 ttttccggag gtagaagatg cttaatgttg cagttttaat gggcaggctt accgccgacc
      961 ctgagttgcg tcataccagc tcggacattc cggtgacttc gtttacaatg gcggttgacc
     1021 ggggctatgt caaagcgggt gccgaacgtc agaccgattt tattgatatc gtctgttggc
     1081 gcggaacggc tgaatttgtc tcacggtatt ttcacaaagg gatgatgatt gccgtcgacg
     1141 gttccattca gacccgttcc tatacagaca aggaaggaaa taagcgcaaa gcctttgaga
     1201 tcgtcgcgcg ggaggttcat tttgcagagc caaagcgcga tagtgaccgg gcagcgtccc
     1261 cgcaggcgtc cgggaatgtt tcatccggct caacacagaa tacacaaaat caggcggcaa
     1321 gcagtgcgcc gccgcaggac aacggggatt tcgtagaaat tgatggggac gatgatttac
     1381 cgttctaatt tgaatgaagg tgcagaaatt gaagtcacag ccacaaatca gcattttaaa
     1441 cgaactgatt gtagataatt ttgccggagg cggcggggcc agcaccggga tagaacttgc
     1501 caccggccgc ccggtagata ttgcgattaa tcatgatccg gacgctattc tgatgcacaa
     1561 gacgaatcac catacacaaa gcattatcag gccagcgtat gggatataga cccggcagag
     1621 gtttgccacg gccagaaggt agcgcttgcc tggttttccc cggattgcaa acacttttca
     1681 aaagcgaagg gcgggaagcc agtagaaaag agtatccggg gccttgcatg gatcgtcctg
     1741 cgctgggccg caaaagtcaa gccgcgggta atcattctgg agaacgtcga ggaattccaa
     1801 acatgggggc ctgtccgaaa gggacgcccg gtgaaatcga agtccggaca gaccttccac
     1861 caatgggaaa gccaactccg ttcactcggt tacgaaattg aacaccggga actgcgggcc
     1921 tgtgattacg gagcgccaac gatacggaaa cggtttttcc tgattgcccg ccgagacggg
     1981 aaaaagattg tatggccgga atcgacacat ggggattcga acagtcctga ggttaaggcc
     2041 gggaaattga aaccgtggcg aacggcggcg gaaatcattg actggagttt gccgtgtcct
     2101 tcaattttcg aatcgtcgga cgagatcaaa cggaaatatg ggataaaggc tgtccgccca
     2161 ctcgctgaaa atactatgcg ccggatcgcc cgtggcatcc agaaatttgt gctggataat
     2221 ccggagccgt ttattctcaa ctataaattt gagaatgatc cagaaagcat gaatcagccg
     2281 ttaagcacta taaccgcggt taacagccat tacattgttt caccggtaat gacagcctta
     2341 gggcagacag gtttttcaaa cgacagatca tacggtgcca atgaaccggt aagaacagtt
     2401 gtcagcaaag ccgaacaatg tcttatcacg cccaccctaa ttcagtacca cagcgaaacc
     2461 gccaagaatg aagcgcgcgg acaatccgtg ggtgaaccaa tcatgaccgt tgatggttca
     2521 aaccgatatg gccttgcatc tgccacattg attcagacag gatacggaga agcaccgggg
     2581 cagaagccaa gagtaccggg actggataaa cctctcggaa cggttgtttc cggtgtaaag
     2641 catgcacttg ttaccgcgtt tatgagtaaa tactatgctg gaggatacca cgggagcgga
     2701 aacaatccag cagacccact gaatacaatc accagcgcag accataacgc ggttgtcacc
     2761 agccatctta ttcagctaaa tcataacact atagcccatc tgcgtaatcc atctccgtat
     2821 tcgcttttac cctttcgcgt atttcatcaa ttttgtccat ccctattcct cctccggctg
     2881 gtagccg
//
LOCUS       DATALY010000284         6760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_62038,
            whole genome shotgun sequence.
ACCESSION   DATALY010000284 DATALY010000000
VERSION     DATALY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62038"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VHR42_07780"
     CDS             complement(<1..201)
                     /locus_tag="VHR42_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3027109.1"
                     /translation="MAKTWYPVIDYLTCAECGTCAAKCSHGVYDPSKAPSPVVKNPEA
                     CIDHCHGCGNRCPVGAITYVGDD"
     gene            complement(215..1228)
                     /locus_tag="VHR42_07785"
     CDS             complement(215..1228)
                     /locus_tag="VHR42_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX3027110.1"
                     /translation="MQILKAIWDFFQYQILAMKWLDGLIGRGLSAIGLDTSARLGGSI
                     EFFLYDVIKITILLCSLIFLISYIQSYFPPERSKRILGRFHGVWANCISALLGTVTPF
                     CSCSSIPLFIGFTSAGLPVGVTFSFLISSPMVDLGSLLLLMSIFGARVAIIYVIVGLI
                     VAVVGGTIIEKLHMERYVESFILSAGSVDIESPDLTKKDRLIYAKEQMLSTFKKVFPY
                     ILVGVGIGAVIHNWIPAEWVSLVLGSKNPFGVVLATLIGVPMYADIFGTIPIAEALLY
                     KGAQLGTILSFMMAVTTLSLPSIIMLRKAVKPKLLALFIAICTVGIILVGYFFNAIQG
                     FII"
     gene            complement(1322..1633)
                     /locus_tag="VHR42_07790"
     CDS             complement(1322..1633)
                     /locus_tag="VHR42_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3027111.1"
                     /translation="MTTVYEEDAKVFKALCDEKRLKILELLRGGEKCACVLLEQLDLG
                     QSGLSYHMKILVESGIVESRQEGKWTHYKISEKGSAYAGTLLKNLTTPNAGAEENTCC
                     K"
     gene            1809..2174
                     /locus_tag="VHR42_07795"
     CDS             1809..2174
                     /locus_tag="VHR42_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027112.1"
                     /translation="MRRPLAYITAPWSADEMENTEDAARYCRKIYDAGFSPICPILFL
                     PLFLNDAIPQEHKDGIDIACNFLRRAHVLVVCGRNVDETVKNDIATAERLHITATTLD
                     GILTVKGQGREKDAEKERR"
     gene            2176..2388
                     /locus_tag="VHR42_07800"
     CDS             2176..2388
                     /locus_tag="VHR42_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027113.1"
                     /translation="MPDYQKMYHSLFNDVTDAIAKLQQAQQKTEEMYMDSKETVLTPF
                     LKKSEEQKNDRKPAPAKKKPPSHDER"
     gene            2425..2916
                     /locus_tag="VHR42_07805"
     CDS             2425..2916
                     /locus_tag="VHR42_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEX3027114.1"
                     /translation="MQEEIENRSVTLIISGAKLTGRVLKAAIAKYLAHRKEKKNAKAR
                     AGPVIPHGRQTVKQLVGQNAGVSNMEITDSNIRSFDRVARKYGVDYAVKQDRSVSPPK
                     YLVFFKARDADALTAALTEFTAKTVNRAKKPSVLSRLRQFKELVKANTVDRVKHKEQE
                     RAR"
     gene            2913..3548
                     /locus_tag="VHR42_07810"
                     /pseudo
     CDS             2913..3548
                     /locus_tag="VHR42_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            4335..6242
                     /gene="ltrA"
                     /locus_tag="VHR42_07815"
     CDS             4335..6242
                     /gene="ltrA"
                     /locus_tag="VHR42_07815"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX3027115.1"
                     /translation="MATKAKARKKPKLRHAEYYDFQSIQDRLYADSEKGEIFKGLVEI
                     ISLPENIRLAYRNIKKNSGSQTAGTDNRTIKDLERFSDEQLIQTVQSKLSWYVPQSVR
                     RVEIPKSSDPSKKRPLGIPTIMDRLIQQCVLQVLEPICEAKFHDQNYGFRPNRSQENA
                     ISKVYNYMQLRHLHYVVDIDIKGFFDNVNHGKLLKQLWTLGIRDKKLLSILSAMLKAE
                     VAGIGFPEKGTPQGGIISPLLANVVLNELDWWLASQWECFPTRHQFKVERKDGSVNRG
                     NKFASLRASSNLKEIAFVRYADDFKIFTSSYQSAVKLFHATQKWLKDRLGLNISPEKS
                     KVINLKEQYSEFLGFKMKVIPRGKRPDGSLKYVVESHIKDKALEKIKGNLDRLIYDIE
                     FPTHGKRTEYNAICKYNAYVLGIHEYYQMATQVCNDLHSIAFSVHKSLKARLHSRIKT
                     AKVVKEKNIPCKTPAVIQERYGKSGQLRYVGGCALVPIGYARCKIAKQKKWTINSYTP
                     EGRAEIHKNLKTIDMDTLHYLMRNPVLYRSVEYNDNRLSLYSAQQGKCAVTGKSLLIG
                     DIHCHHKVPKHMGGTDKYRNLVLVCEKVHRLIHATNPDTVEKYMNALKLTSKQSEKLN
                     RLRSLVSVENC"
     gene            6278..>6760
                     /locus_tag="VHR42_07820"
     CDS             6278..>6760
                     /locus_tag="VHR42_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEX3027116.1"
                     /translation="MSRKIHARFGAGENPATISKDHLSLSFAYIHSEKDILKLVTALI
                     TNTKGEGKEGDDFWVKAETLLYTALIGYIHYEAPVEEQNFATLIEFINASEVREDDEE
                     FKNPVDLMFEALEKEKPNHFAVRQYKKYKLAAGKTAKSILISCGARMAPFDIQELRDL
                     T"
BASE COUNT         1936 a         1682 c         1703 g         1439 t
ORIGIN      
        1 atcgtcgcca acataggtaa tcgcgccgac cggacagcgg tttccgcagc cgtggcaatg
       61 gtcaatgcag gcctccggat tttttacgac gggtgacgga gcctttgagg gatcataaac
      121 accgtgggaa cactttgcag cacaggtacc gcactctgcg caggtcagat agtcaataac
      181 aggataccat gtctttgcca taatactttc ctccctatat gatgaatccc tgaatcgcgt
      241 tgaaaaagta gcctacaaga ataataccga ccgtacaaat tgcaatgaac agcgccaaca
      301 gctttggctt caccgctttg cggagcatga taatggacgg cagggacaac gtggttacgg
      361 ccatcataaa ggagaggatc gtgccaagtt gcgctccttt gtataacagt gcctccgcaa
      421 tcggaatggt accgaaaata tctgcgtaca tcggcacacc gatcagggtt gccagaacaa
      481 caccaaaggg atttttgctg ccgagcacaa gcgacaccca ttctgctgga atccagttgt
      541 gaatgaccgc gccgatgccg acaccgacta aaatataagg aaacactttt ttgaaagtgg
      601 acagcatttg ctccttggca taaatcaacc ggtctttttt tgtcaggtcc ggagactcaa
      661 tgtctacgct gcccgctgac aggataaaac tttcaacata ccgctccata tgcagcttct
      721 caataatcgt gcctccgaca accgcgacaa tcagcccaac aataacataa ataattgcta
      781 ctcttgcgcc aaaaatgctc atcagcagaa gcagtgatcc gagatctacc attggggaag
      841 aaatcagaaa cgagaacgtc accccaaccg gaagccctgc ggatgtaaac ccaatgaaca
      901 gtggtataga ggaacaggag cagaacggcg tcaccgtacc aaggagcgcc gaaatgcagt
      961 ttgcccagac accgtggaaa cgaccgagaa ttcgtttact gcgctccggt gggaagtagc
     1021 tttgaatata actgatgaga aaaatcaggg aacacagaag tatcgtaatc ttgattacgt
     1081 cgtacaggaa gaattcaatg cttccgccca gccgtgccga cgtatcaagt ccaatagctg
     1141 ataggcctct tccaatcagc ccatccagcc atttcatggc aagaatctga tactggaaga
     1201 aatcccaaat tgcttttagt atttgcactg gataaaaacc tcactttcga taaacggaaa
     1261 atcacatcaa ataatttcga tgtattctgc tctaaaaaag ccatcgtccg gatggctttt
     1321 cctatttgca gcaggtgttt tcttctgcac ctgcattcgg tgtggtcaaa ttcttgagca
     1381 aagtaccggc ataggcgctg cccttttcac tgattttgta atgcgtccat ttgccttcct
     1441 gccgactctc cacaataccc gattcaacca aaatcttcat gtggtaagaa agtcccgact
     1501 gccccaaatc cagctgttcc agcaaaacgc aggcacattt ctccccgccg cgcagaagtt
     1561 caagtatttt taaccgcttt tcatcgcata atgctttgaa cacttttgcg tcttcctcat
     1621 aaacggtcgt caagttatca cctcatatca aaattatttg atgtgttatt agtatatgcg
     1681 tcaaatcaag ttttgtcaat atgaaatacg taatatcgtt attttgttta cggctcacac
     1741 ggacgatacg cgcgtccgat gcgggccgtt ttatatatta acaaactttt gaaaggagta
     1801 attttaccat gagacgaccc ctcgcataca tcaccgcccc gtggagtgca gatgaaatgg
     1861 aaaacaccga ggacgcggcg cggtactgcc gcaaaattta cgacgcgggc ttttcgccca
     1921 tctgcccgat cctgtttctg ccgcttttcc tcaacgacgc catcccgcag gagcacaagg
     1981 acggcatcga tatcgcctgc aactttctgc gccgcgctca tgtgctggtg gtctgcggca
     2041 gaaacgtgga tgaaaccgta aaaaacgaca tcgccacggc ggaacggcta catatcaccg
     2101 ctaccacgct ggacggcatt cttaccgtca agggacaggg ccgggaaaaa gacgcggaaa
     2161 aggagcggag ataacgtgcc ggattatcag aaaatgtacc actcgctgtt caatgatgtg
     2221 accgacgcta tcgcaaagct ccagcaggcg cagcagaaaa ccgaggaaat gtacatggac
     2281 agcaaagaaa ccgtcctcac cccgtttttg aaaaagagtg aggaacagaa aaatgaccgg
     2341 aaaccggccc cggcaaagaa aaagccgccc tctcacgacg aacggtgacg gagggcggct
     2401 ttcccatgac aggaggtgtt ttgtttgcag gaagaaattg aaaaccgttc cgtgacgctt
     2461 attatcagcg gcgcgaagct cacgggccgg gtgctcaaag cggcgattgc caagtatctg
     2521 gcccaccgga aggagaagaa aaacgccaag gcccgtgccg gtcccgtgat cccccatggc
     2581 aggcagacgg tgaaacagct tgtcgggcag aacgcgggcg tgtcgaacat ggagattacc
     2641 gacagcaaca tccgttcctt tgaccgcgtg gcgcggaaat acggcgtgga ctacgccgtc
     2701 aagcaagacc gcagcgtgtc gccgcccaaa tatctcgtct ttttcaaggc acgggacgcg
     2761 gatgctctca ccgccgcttt aaccgagttc acggcgaaaa cggtgaaccg ggcgaaaaag
     2821 ccctccgtcc tctcgcggct ccgtcagttc aaggagctgg tcaaagcgaa taccgttgac
     2881 cgggtgaagc ataaggaaca ggagcgggcg cgatgagtga gaaaacaaaa aagctgctcc
     2941 tgatgaacct tccgtacctg tttgccgcgc tgttcgccac caagttcggg cgggcgtggc
     3001 ggctggccgc aggcgcagac gcttcgggaa agctcctgca cctgatggac ggccttaccg
     3061 ccgccttttc ctccccgctg ccgagctttt atccggccga cctcggcgtg ggcgttcttc
     3121 ttgctgccgc tctccgtttg gcggtttatg tcaaaggccg gaacgccaag aaattccgca
     3181 agaacgtgga atacggctcc gctcgctggg gcaaggccga ggacatcaaa ccgtacatcg
     3241 accctgtttt tgaaaacaac gtcattctca cacagacgga acggctcacc atgaacagcc
     3301 gaccgaagga ccccaaaacg tcccggaaca aaaacgtgct ggtcgtcggc ggctccggtt
     3361 ccggcaagac gcggtttttc atcaagccga atctgatgca gtgcgattcc aaggattatc
     3421 cgacgagctt cgtggtcacg gacccgaagg gcagcatcgt cgtagagtgc ggaaatctcc
     3481 tgcggcgtag gggctaccga atcaaaattc tcaacaccat caacttcaag aagtcgatgc
     3541 actacaatcg tgaagcgaca cgttgctcgc agataaactt cgcgtgggag tgatcctgca
     3601 aagaggtaaa agcgacagga aaacacagtt ttcttaactg atgcgccaag gagtggaatg
     3661 aaggggtaac gccctgaaac gctccctctg atactgcgtt ggaaagaacg gagtgcaatc
     3721 tccgggactg acacagcacg ctaaagtcgg ctatttgcgc ccgctactgt cccggacggg
     3781 ataaggcaaa accgagccag aggtggccgg aggcgttagg ccgggggtct taaaaatctg
     3841 cccatggcaa gaatgtggtt cgcagccact gacaaaagtc cgaatgtacg ggtctaaaag
     3901 ttgcaaacgg tagaaatgcc gtttccgcga aagcggagca tgtggggaaa agtaaaattg
     3961 gttgttatga aattccctgc ggcgttacag gcgccgccga gcatgcaggc tcagaggaca
     4021 gcctaaaggt ggatacgcac agataggagc atcggaacgt ggaaaatttc ggacgtggag
     4081 tgattgtaca cgatgaaacg ctggaaagga aaaccatgcc gtcaactttg aaggtgtggc
     4141 ataacttttc ttaatccgaa atgaaagcgc agccatgata ccgatgaagc cgtgaattaa
     4201 aggaagcggt ggagggacgg gctgtagtca aagatgaatt gcaataaata tcatgtcatc
     4261 actaacaggc atccgtaggt tcgagtagga ctaagaaagg cgaaaacccg cgaaagcagg
     4321 tgactaagcc gactatggca acgaaagcga aggctcgaaa gaaaccgaaa ctgcgccacg
     4381 ccgaatatta tgatttccag agtattcaag acaggctcta cgccgacagc gaaaaaggcg
     4441 aaatattcaa aggccttgtg gagattatct ccctgccgga aaatatccgc ctcgcatatc
     4501 gcaatatcaa gaaaaacagt ggaagtcaga cggctggcac ggacaatcgc acgatcaaag
     4561 atctggagag attttctgat gaacagttga tacaaaccgt acaaagcaaa ttaagctggt
     4621 atgttcccca gagtgttcgg cgggtggaaa ttcctaaaag cagcgacccc tcgaaaaagc
     4681 gccctctcgg catccccacc attatggaca ggctcattca acaatgtgtg ctgcaagtgt
     4741 tggaaccgat atgtgaagca aaatttcatg accagaatta tggctttcgg ccaaatagaa
     4801 gtcaggaaaa cgccatttca aaggtataca actacatgca gctacggcat ctgcattacg
     4861 tggtggatat cgacatcaag ggcttcttcg ataacgtgaa ccacggcaag ctgctcaaac
     4921 agctttggac actgggtatc cgtgataaaa agctgctgag tatcctttcg gcaatgctca
     4981 aagccgaggt ggccggtatc ggtttcccgg aaaagggcac accgcagggc ggcatcatct
     5041 cgccgctcct ggctaatgtg gttctcaatg aactggactg gtggctggca agccaatggg
     5101 aatgcttccc gacgcggcat cagttcaaag tagaacggaa ggacgggagc gtcaaccggg
     5161 gcaacaaatt tgcgtcgctc cgagcatcca gcaatttaaa agaaattgca ttcgtccgat
     5221 acgccgacga ttttaaaatc tttaccagca gttatcaaag tgcggttaag ctgttccacg
     5281 ccacccaaaa atggctgaaa gaccgacttg gcttgaatat cagtcccgaa aaatccaagg
     5341 tgattaacct gaaagaacaa tattcggaat ttttgggatt caagatgaag gtgattccgc
     5401 gaggcaaacg cccggacgga agcctcaagt atgtggtcga aagtcatatt aaggataagg
     5461 cgcttgagaa aatcaaggga aatctcgacc ggctaatcta tgacattgag ttcccgacac
     5521 acgggaagcg tacggaatac aacgcaattt gcaaatacaa tgcgtatgtg ctgggaatcc
     5581 atgagtatta ccagatggcc acgcaggttt gcaatgattt acactctata gccttctcag
     5641 tccataagag cctcaaagcg cggcttcaca gcaggattaa gactgccaaa gtagtgaagg
     5701 aaaagaacat tccatgtaaa acaccggctg ttattcaaga gcgatatggc aaaagcggtc
     5761 aactgcggta tgtgggaggc tgcgctcttg tgcccattgg gtacgcaagg tgcaaaatcg
     5821 ccaagcaaaa gaagtggacg attaattctt ataccccaga gggcagagca gaaattcata
     5881 aaaatctgaa aacgattgat atggacacac tgcactattt aatgcgaaat cctgtcctat
     5941 accgttccgt ggaatacaac gacaatcgtc tgtcgctcta ctcggcccag cagggcaaat
     6001 gcgcggtaac gggaaaatct ttgctgattg gagacattca ttgtcaccat aaagtgccca
     6061 aacacatggg cggcacggat aaataccgaa acctcgtact cgtatgcgaa aaagttcatc
     6121 gtctcattca cgccacaaat ccagatacag tcgagaaata catgaatgcg cttaaactta
     6181 cgtcaaagca gtctgaaaaa ttgaatcggc taagaagcct tgtttctgtt gagaactgtt
     6241 agtatgaaac gcaattacat ctttgatgga aagccgggtg agtcgaaaga ttcatgcccg
     6301 gttcggggcg ggggaaaatc cggcgacaat atcaaaggat cacctatcgc tatccttcgc
     6361 gtacattcat tcggaaaaag acattttaaa gctggtcacg gcgcttatca ccaacaccaa
     6421 gggtgaaggt aaggaaggag atgatttctg ggtgaaagcc gagactctcc tttatacggc
     6481 gctcatcggc tacatccact atgaggcccc ggtcgaggaa cagaatttcg ccacgctgat
     6541 cgaattcatc aacgcttcgg aagttcgaga ggatgatgag gaattcaaaa atcctgtgga
     6601 tctcatgttt gaggcgctgg aaaaggaaaa accaaatcac ttcgctgtcc ggcagtataa
     6661 gaaatataag ttggcggcgg gcaagacagc aaaatcgatc cttatttcct gtggcgcaag
     6721 aatggccccc ttcgacattc aggagctgcg tgacctgaca
//
LOCUS       DATALY010000285         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_493454,
            whole genome shotgun sequence.
ACCESSION   DATALY010000285 DATALY010000000
VERSION     DATALY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493454"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VHR42_07825"
     CDS             complement(<1..239)
                     /locus_tag="VHR42_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027117.1"
                     /translation="MVLSIFSLDTVGASAATYNPVDSQYYNNGATDQISLDLVNGPVV
                     LLIDSGMNAFFQGSFQEYNSSQVTIHTIDSGDSVR"
     gene            complement(390..638)
                     /locus_tag="VHR42_07830"
     CDS             complement(390..638)
                     /locus_tag="VHR42_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEX3027118.1"
                     /translation="MSKIINVAPKDGYVLGISFSDGSVLDFCMKRQVKTAQFHEIKEP
                     EAFKKVYFDDKSIFWQPGSSKQLRMTLDSILFTLRDVP"
     gene            complement(747..1976)
                     /locus_tag="VHR42_07835"
     CDS             complement(747..1976)
                     /locus_tag="VHR42_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3027119.1"
                     /translation="MERFYQGKSGIDEAERDRILAEILCVRKFTKFNRLEEMHSYNTE
                     ILRLLNGRQSSIMLQVNEFTFGSPHYLYIYFRDAGSIKKLIELCQRNGHSAFSNGCGT
                     GSDSLALAEYACETGDYETALHESQKAVYKAETKSQWSVVICAEFNEIRAYAATGKAD
                     VAYKTLCQMREGVEKLSNPVYDSTFQLCSGYFYSVLGRSEKIPVWLRNGDLSSLSLFF
                     GGLGFDKLVYEKALLAQGKFLKLEALSDSFDVGFGVFQNRLGFLHNAVMLAAARGRLY
                     GAKGGLANLRRALNLACPDEIMLPFAECATYLLPIFAEMPETEKREVFTKRVMDCCKQ
                     YEEHMDGVLRKAPELTKRETEVLALLAKGLNRNDIARRLVVSPATVKTHLQLIYRKLE
                     ADGKVSALNIAKMNGLI"
     gene            complement(1978..3216)
                     /locus_tag="VHR42_07840"
     CDS             complement(1978..3216)
                     /locus_tag="VHR42_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027120.1"
                     /translation="MNRTQHLIRERMNRLLSAITNYPLTILEAPAGYGKTTAVQDYLD
                     SQKIRPLWISFRPMVECSEYCWAKVAAESEKMDSAVGTTLKSLGFPVDAPQRNRILSI
                     LNRLELVEPTVVVLDNYELVTDPLLSRLLLQVAEDEIENLHLVVLARDTTLLDFVPLL
                     LSEKCRVISQQQIKFTVDEVRNYGTLTGSSVTDEEIERIAEYTDGWISMIYMLILGAE
                     ENIPVGLTQTLDELIDQVLYRVHDPETQQFLLQLSVMDGFTAEQATFVTGNAQAYKLL
                     KQLQKKNAFIFYDEREKEYKIHNVLLDFLRARQNFQEKEEHALYRNLGEWLLRENDPR
                     PAYSCLARAGDAERILTHLNRTQPIRNLYTDFDGADELFASAPKELLYKKSDCLSALS
                     VLFNCISQAIGRRRYDTAVG"
BASE COUNT          827 a          872 c          808 g          919 t
ORIGIN      
        1 atgcggacgg agtcgccgga gtctatggtg tgaatcgtta cctggctgct gttatattcc
       61 tgaaacgaac cctgaaagaa cgcgttcata ccgctgtcaa tcaaaagtac cacgggaccg
      121 ttgaccaggt ccaaagaaat ttgatctgta gcgccgttat tataatattg actatctact
      181 ggattgtagg tggcagccga agcgccgact gtgtccagcg aaaagatgct gagtaccatt
      241 gcaatgctta taaatagaga taacgtcctc ttatgaattg aatttctgtt cattattttt
      301 ctcatttctc attaaagctg gttttttggt tttgcgatca atttttggtg tttatcgttt
      361 tccgcttgtt ctaaaccgtt gaaaagcttc tacggcacgt ctcgcagtgt gaataggatg
      421 ctgtcaagcg tcatacggag ctgtttggaa ctgcccggct gccagaaaat gcttttatcg
      481 tcgaagtaca cctttttgaa cgcctccggc tccttaatct cgtgaaactg cgcggttttc
      541 acctgccgct tcatgcaaaa atcaagcacg ctaccgtcac tgaacgagat tcccaacaca
      601 tagccgtcct tcggggcgac attgataatt ttactcatcc tccattcaac tcctttcgga
      661 tttggactta aaaaatccca tactgcaatt ttacagtatg ggatttagga tgtatatccg
      721 ccatatggcg tatttattga acaaaattaa atcaagccgt tcattttcgc gatgttgagg
      781 gctgaaacct taccgtccgc ttccagtttc cgataaatca actgcaaatg cgtcttgact
      841 gtggcggggg atacgactag ccgccgcgcg atgtcgtttc gattcagccc cttcgccagc
      901 agcgcaagga cttcggtttc ccgctttgtc aattccggtg ctttgcgcaa gacgccgtcc
      961 atgtgctctt catattgttt gcagcagtcc atgacacgct ttgtaaatac ctcccgcttt
     1021 tccgtttccg gcatctccgc aaaaatcggg agcagatagg tggcacattc tgcgaacggc
     1081 agcataattt catccgggca cgcaagattg agtgcccgcc ggaggttagc aaggccaccc
     1141 ttcgcgccgt aaagtctacc gcgggcagcg gcaagcataa ccgcgttgtg caggaaaccc
     1201 agccggttct gaaaaacgcc gaacccgaca tcaaaactgt cgctgagcgc ctcaagctta
     1261 agaaactttc cttgcgccaa aagcgccttt tcatagacga gcttgtcaaa tccgagaccg
     1321 ccaaaaaaca ggctgaggga ggaaaggtca ccgtttctga gccaaacggg aatcttttcc
     1381 gaccgaccca gtacggaata gaaatacccg ctgcacagtt ggaacgtgga gtcatagact
     1441 gggttgctga gtttttccac gccttcgcgc atctggcaca gcgttttgta tgccacatcc
     1501 gctttccccg tagcggcata cgcacgtatc tcgttgaatt cagcgcagat caccacactc
     1561 cactgcgatt ttgtctcggc tttgtagaca gctttttggc tttcgtgtag ggccgtttca
     1621 taatcgccgg tttcgcaggc gtattccgcc agcgcgaggg aatcactgcc agtgccgcag
     1681 ccgttagaaa aagcggaatg gccgttccgc tggcagagct cgataagttt ttttatcgaa
     1741 cctgcatcgc ggaaatagat gtagagatag tgcggcgaac cgaaggtaaa ctcatttacc
     1801 tgaagcatga tggaggactg acgcccgttt aagaggcgga gaatctcagt gttgtaggag
     1861 tgcatctctt ccaaccggtt gaacttagtg aatttacgca cgcacaggat ttcggcaagg
     1921 atgcggtctc gttccgcttc gtcgatcccg ctttttcctt ggtaaaaccg ttccaaatca
     1981 tccaaccgcc gtgtcatatc tgcgacgacc gattgcttga ctgatacaat tgaatagaac
     2041 agatagcgca gataagcaat cggatttttt gtacagcagt tctttcggtg cggaggcaaa
     2101 cagttcatcc gccccgtcaa aatccgtata aagattccgg atcggctggg tgcggttcag
     2161 atgggtgaga atccgttcgg cgtcccccgc tctggcaaga caggaataag ccggacgcgg
     2221 atcattttcc ctcagaagcc actcacccag attgcgataa agtgcgtgtt cttctttttc
     2281 ctgaaaattt tgccgtgcac gcagaaagtc aagcagcacg ttatgtattt tgtattcttt
     2341 ttcccgctcg tcataaaaaa tgaaagcgtt cttcttttga agttgtttca gaagcttata
     2401 agcctgcgca tttccggtaa cgaaggtggc ctgctctgcg gtaaagccgt ccatgacgga
     2461 aagctgaagc agaaattgct gtgtttccgg gtcgtgaaca cggtacagaa cctgatcgat
     2521 gagttcatca agagtctgcg tcaggccgac cggtatattt tcttccgcgc caaggatcag
     2581 catgtagatc atgctgatcc atccgtcggt atattccgcg atccgttcga tttcctcgtc
     2641 cgtgacggag ctccctgtca gcgtaccgta atttctgacc tcgtccaccg tgaatttaat
     2701 ttgctgctga gaaataacgc ggcacttttc gctcagcagc agcgggacga aatcgaggag
     2761 ggtggtatca cgcgccagaa cgacaaggtg caggttctcg atttcatcct ccgctacctg
     2821 caggagcagc cggctcagaa gagggtcggt tacaagttca tagttatcca acaccactac
     2881 ggttggttcg accagctcca gccggttgag aatggataaa atccggttcc gctgcggcgc
     2941 gtccaccgga aacccaaggc ttttcagcgt ggttcccacg gcgctgtcca ttttctcaga
     3001 ttcggcagct acttttgccc agcagtattc ggaacactcg accataggac ggaaggaaat
     3061 ccaaagtggc ctgatttttt gggaatccag ataatcctga acggcggtcg tcttgccgta
     3121 gcccgccggg gcttccagaa ttgtcagcgg atagttggtg atggctgaga gcagccgatt
     3181 catccgttcc cgaatcaaat gctgcgttct attcatggag gagccccctt tctcgccact
     3241 ttatcctatc cgaaaactca gactcttact taaggcaaaa ttccaaatca ttatacttat
     3301 atgacaatat tttaccatat tttttatatt tcgtcatcta tttcataaaa tcacatcttt
     3361 accaggtcga cagttattgt cacattcaag ccccttttac tgaagatttt ataataaaca
     3421 tactaa
//
LOCUS       DATALY010000286         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_149127,
            whole genome shotgun sequence.
ACCESSION   DATALY010000286 DATALY010000000
VERSION     DATALY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149127"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2943)
                     /locus_tag="VHR42_07845"
     CDS             complement(<1..>2943)
                     /locus_tag="VHR42_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027121.1"
                     /translation="PAVYLLARSFCNAARGSIDAAKIEEFLGGAQKQRSMESAELSGF
                     GVMLRAALIEGAARACVMNLEDQERKILLSDAVKTLNFLTTFDFSEIIERQSGIERIM
                     TEDPAGIYTRMDERSRGVYRDMIAKIARKRKMSETDVAAKTVEMARMGKTSRERHVGY
                     YILERELDRPKSTRRGKIYLFLLWSVPAILGILLGLAFRKVWLPFLVYLPLWEIVRPI
                     IEYETMKGVPATYLPRLENDGVVPEDAPTLVVISTLLSSPQKIEEFVKKLEQFYYSNG
                     WGAILFGLLADLKESKLPEQPEDKAIKTAAIKAIRKLNERLGPHFCIFIRSRRLSRTQ
                     GKFSGWERKRGAIIELIRYMKGISTSITTVEGDARMLHSIKYLITLDADTGLLIDTAS
                     EMVSVAMHPLNEPEIAEGENKVGRGYGILSPKISIDLESAGRTPFSRVMAGCGGVTAY
                     DNAAGNAYQDLFGEGIYAGKGLINVDAFYSVLDHALPENRILSHDILEGCYMRVGFVS
                     DVEMTDGFPPKPAPWFDRLHRWIRGDWQNISYISGRIKTAGGTVKIPFGNLSRFKLAD
                     NLRRSVTPVASVLGVAFSAFTGLKLSLTLILIAFLSIAGPGLWSAALAVARGGFSMLS
                     RKYHCKVLPQAVNSVYQGILLYLFLPYHALIAADGVIRALWRQMSGRKMLEWVTAAEN
                     EARVGGAAENLRRFWVCTVTGVVFLAAAVSPLARVAGAFWIITPFVAWMSGRPMSQSS
                     AGLTDDDKEKLRSYTAAMWRYYEDYVGEKDHFLPPDNVQEAPVHVVAHRTSPTNIGLY
                     LLSTLAARDLRLIDSEILLERVTATLSTIEKLGKWKGHLYNWYDTVTLEPLKPRYVST
                     VDSGNLLCCLTALSEGLKEYAYLGPASELTNRIQKLIDDVDLGVLFNKRRKLFHIGYD
                     IEKDCLSEIYYDLLMSEARMTSYYAVARRIAPKRHWGALGRTLTRQGGYTGPISWTGT
                     MFEYLMPH"
BASE COUNT          686 a          860 c          708 g          689 t
ORIGIN      
        1 ggtggggcat gagatattca aacatggttc ccgtccacga gatcggcccg gtatatccgc
       61 cctggcgggt aagcgttctg ccaagcgctc cccagtggcg cttgggcgcg atacgcctgg
      121 ccaccgcgta gtaactggtc attcgcgctt cactcataag caggtcgtaa taaatttcgc
      181 tcaaacagtc cttttcaatg tcatagccga tatggaacag tttacggcgc ttattgaaca
      241 gtacgccgag gtcgacgtcg tcaatcagtt tttggatgcg gttcgtaagt tcgctggccg
      301 ggccaaggta tgcgtattct ttcaagcctt cggaaagcgc cgtcaggcaa cagagcagat
      361 ttccgctgtc cacggtcgag acatatctgg gtttcagagg ttcaagcgtc accgtatcat
      421 accagttgta aaggtgccct ttccatttcc ctaatttttc aatcgtcgaa agagtggccg
      481 tcacccgctc aagcaggatc tcactgtcaa tcagcctcag atccctcgcg gcaagcgttg
      541 agagaagata cagcccgata ttggtcggcg aagtccgatg ggcaactaca tgaacaggcg
      601 cctcctgcac attgtcgggc ggcagaaagt ggtctttttc acccacataa tcttcgtaat
      661 atctccacat cgccgccgta taggagcgaa gcttttcctt gtcatcgtcg gtcagtccgg
      721 ccgacgactg agacatcggc ctgccggaca tccacgcgac gaaaggcgtg atgatccaaa
      781 acgctccggc gacgcgtgcg agaggagaga cggctgccgc cagaaaaaca acccccgtca
      841 ctgtgcagac ccaaaatctt ctgaggtttt ccgcggctcc gccgacccgc gcctcatttt
      901 ccgcggccgt cacccattcc agcatctttc ttcccgacat ctgccgccac agcgcccgga
      961 tcactccatc ggccgcgatc agcgcatgat agggcagaaa caaatacagc aggatgccct
     1021 gataaacgga attgaccgcc tgcggcagca ccttgcagtg gtattttctt gacagcatgg
     1081 aaaatccccc gcgtgcaacc gccaaagcgg cgctccaaag cccaggtccg gctatggaca
     1141 gaaaggcgat caagatcagc gtcagcgaaa gtttcagccc ggtaaaagca gaaaacgcaa
     1201 cgccaagaac ggaagcgacc ggcgtcacag acctcctcag attatcggcc agtttaaacc
     1261 tgctgagatt tccaaagggt atttttaccg tcccgccggc cgttttgatt ctgccggata
     1321 tataagaaat attctgccag tctccccgga tccaccgatg cagccggtca aaccaggggg
     1381 caggcttggg cggaaaaccg tcggtcattt caacatcgga aacaaaaccg acccgcatat
     1441 aacagccctc cagaatatcg tggctgagaa ttctattttc aggaagggca tgatccagaa
     1501 cgctgtaaaa agcgtcaacg ttgatcaggc ctttcccggc atagattccc tcgccaaaca
     1561 gatcctgata ggcgttgccg gccgcattat cataagccgt gaccccgccg cagccggcca
     1621 tgacccgcga aaacggagtc cgtccggctg attcaagatc aatacttatt ttgggggaga
     1681 gaattccata gcctctgccg actttgtttt ctccttcggc aatctcgggc tcattcagag
     1741 gatgcatggc aaccgacacc atttccgaag ccgtgtcgat cagcagcccc gtatccgcgt
     1801 ccagcgtaat taaatattta atggaatgaa gcatccgcgc gtcgccttcc acggtcgtaa
     1861 tcgaagtaga aatccctttc atatagcgaa tcagctcaat gattgcgccg cgttttcgtt
     1921 cccagcccga aaatttcccc tgtgtccggc taagtctgcg gcttcggata aaaatacaga
     1981 aatggggccc aagcctttcg ttaagcttcc ggatcgcttt aattgccgct gttttaatcg
     2041 ctttatcttc cggctgctcg ggcagctttg attcctttag gtctgcgagc aagccgaata
     2101 gaatcgcgcc ccatccgttg gaataataaa actgctcaag ctttttgaca aattcttcga
     2161 ttttctgagg cgagcttaac agagtggata ttaccaccag agtgggagca tcctccggca
     2221 ccaccccgtc gttttccagc cggggcaggt aagtggccgg cactcctttc atcgtttcat
     2281 actcgataat cggccttaca atttcccaaa gcggcaaata aaccaaaaag ggcagccata
     2341 cttttcgaaa ggccagcccc agcaggattc ccaatattgc cggaacgctc catagaagga
     2401 acaaataaat ttttccgcgc cgggttgatt tgggtctgtc caattcccgt tcaagaatat
     2461 agtagccgac atgccgctcc cggcttgtct tccccatccg ggccatttca accgttttag
     2521 cggccacatc ggtctccgac atttttcgtt ttcgtgcgat ttttgcgatc atatcgcggt
     2581 agactcctct ggaacgctca tccatcctgg tatagattcc ggcagggtcc tccgtcatga
     2641 ttctttcgat tccgctttgt ctttcgataa tttcggaaaa atcaaaggtc gtcaaaaagt
     2701 tcagcgtctt cactgcgtcg gaaagcaaaa tttttctttc ctgatcttcc aggttcatga
     2761 cgcaggcgcg cgccgcccct tcgatcagag ccgcgcgcag catcactcca aaccccgaaa
     2821 gctcggcgct ttccatgctt ctctgctttt gcgctccccc aagaaactcc tcgatttttg
     2881 ccgcgtcaat cgatcccctc gccgcgttgc agaaactgcg ggcaagcagg tagaccgccg
     2941 gcg
//
LOCUS       DATALY010000287         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_667869,
            whole genome shotgun sequence.
ACCESSION   DATALY010000287 DATALY010000000
VERSION     DATALY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667869"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VHR42_07850"
     CDS             <1..324
                     /locus_tag="VHR42_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3027122.1"
                     /translation="KDFYKNILISRGIDVQIPPKEDIDRIHKIINQELIIGEIKDSSR
                     SYFSNVITGLKQQGAQGVILGCTEIGLLVKQKDSSLPVYDTALIHADAAAEYAMMEDE
                     NDGTI"
     gene            311..535
                     /locus_tag="VHR42_07855"
     CDS             311..535
                     /locus_tag="VHR42_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX3027123.1"
                     /translation="MAQYEYKFIEVPRSPKFVKQIERLNSDDFKKCEDIIVCEAKSGW
                     RLKQVVVPYQERFGLFTAKGYEIILEKEIM"
     gene            559..918
                     /locus_tag="VHR42_07860"
     CDS             559..918
                     /locus_tag="VHR42_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3027124.1"
                     /translation="MKLNWVTLRVNDLEKSLCFYTKLLNLDIAAKFGSPEHPIVMLGK
                     AEEAKLELIHESHPQIDPSGNGVSIGLEADDLDRLIGVLRENGYQATGPVSPNPNIRF
                     FFVRDPDGYTVQLVEQI"
     gene            1103..2236
                     /locus_tag="VHR42_07865"
     CDS             1103..2236
                     /locus_tag="VHR42_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX3027125.1"
                     /translation="MEKAKVYFTDFRTIAYGDGLPTKLKKLIKKAGIGQIDMDGKFVA
                     VKMHFGELGNISYLRPNYARAVVDVIKELGGKPYLTDCNTMYPGSRKNALEHLECAWQ
                     NGFTPLTVGCPILIGDGLKGTDDIAVPVRGGEYVQEAKIGRAVMDADVFISLTHFKGH
                     EATGFGGTIKNIGMGCGSRAGKTEQHSGGMANIVPEKCRGCRKCQKECANGGLEFDEV
                     KKKMTVNEDNCVGCGRCLGACNFDAIEFNDSAANALLNCRMAEYTKAVVDGRPNFHIS
                     LVVDVSPNCDCHGENDAPILPDIGMFASFDPLALDQACADACLKAQPMPGSQLSDNMA
                     KADFVDHHDHFTNSTPESEWLSCLEHAEKIGLGTRSYELIPMK"
     gene            2348..>3768
                     /locus_tag="VHR42_07870"
     CDS             2348..>3768
                     /locus_tag="VHR42_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX3027126.1"
                     /translation="MLLLSAEKISKSYVDKILLNDVAFGINSGEKIGLIGINGAGKST
                     LLKIIAGLETPDTGTVTKFGGVRVGYLPQNPLFEDGITVLQQVVKGISEQQRESKEYE
                     CKSILTRLGINEFDKPLSQLSGGQKKRVSLASALVTPVEVLILDEPTNHLDNDMVDWL
                     ETYLAGYNGALLMVTHDRYFLDRVTNKIIELQNGNLYAYQANYTSYLELKAERESMAA
                     ASERKRQTLLKKELAWISRGARARSTKQKFRVERYEEMSAQSAEPEQENLQMSSMSSR
                     LGKTIIEIGGISKAYGGRQLILNFSYNLLRRDRIGIIGPNGCGKSTLLKMIVGTVSPD
                     EGDVRIGETVRIGYFSQECEEMDPNQRVIDYVKEISSEVVTPSGTLTASQMLETFLFS
                     SDLQYSLIKKLSGGERRRLYLLGILMQAPNVLLFDEPTNDLDIQTLIILEDYLQNFSG
                     AVIAVSHDRYFLDKIAEHVFSIE"
BASE COUNT         1045 a          884 c         1014 g          825 t
ORIGIN      
        1 aaagattttt ataaaaatat actgatcagc cgcggaatcg atgttcagat tcccccaaag
       61 gaagatatcg accggattca caaaattatc aatcaggaat taatcatcgg ggaaatcaag
      121 gactcttccc gcagctattt cagcaatgtc ataaccggat tgaaacagca gggcgcacaa
      181 ggagttattc tcggctgtac ggaaatcggc ttgcttgtaa agcaaaaaga cagttcttta
      241 ccggtatacg ataccgctct catccatgcg gacgccgccg ccgaatatgc aatgatggaa
      301 gatgaaaacg atggcacaat atgaatataa gtttattgaa gttccccgct ctccgaaatt
      361 cgtcaagcaa atcgaacggc ttaactccga cgattttaaa aagtgtgagg atatcattgt
      421 ctgtgaagcc aaaagcggat ggagactgaa acaagtcgtc gtaccctatc aggagcggtt
      481 cgggctcttt acagcaaagg gctacgagat tattttagaa aaggaaatca tgtgaatttc
      541 aattaggaga taaatgaaat gaagctaaac tgggttacct tgagagtcaa cgatctggaa
      601 aaaagcctct gcttctacac aaaactgctg aatcttgaca tcgcggcaaa gttcggcagc
      661 ccagagcatc cgattgtcat gttagggaaa gcggaagagg cgaaactcga attaattcac
      721 gaatcccatc cgcagataga tccttccgga aatggggttt ccatcggctt ggaagcggac
      781 gatctggacc ggctgatcgg tgtgctgcgg gaaaacggat atcaggcaac aggcccggta
      841 tcgccgaatc cgaacatccg atttttcttt gtccgggacc ccgacggata tactgtgcag
      901 ctggtagagc aaatataaaa gacgcttagc aggctaccgc cctgaagaac gctgtgaggg
      961 aaacagcagt taaattttta tttgcccttg acttaaagtt aactctaacg tgtataattt
     1021 taaacggatg gcccgtcccg ggaagggaca cggtcatgct ttgccggaaa aaacgatgat
     1081 tccaaaacag gagtggatta acatggagaa agcaaaggtc tattttaccg atttccgcac
     1141 gattgcttac ggtgacggac ttcccaccaa gctgaaaaag ttaattaaaa aagccggcat
     1201 cggccagatt gatatggacg gcaaatttgt cgccgttaag atgcatttcg gcgagttggg
     1261 gaacatcagc tatcttcgtc ccaattacgc cagagccgtg gtcgatgtga taaaggagct
     1321 gggcggaaag ccttatttga ccgactgcaa caccatgtac cccggcagcc gaaaaaacgc
     1381 tctggagcat ttggaatgcg cttggcaaaa cggttttaca ccgctcacgg tgggctgccc
     1441 catcctgatc ggcgacgggc tgaagggtac cgacgatatc gcagttcccg ttcggggcgg
     1501 agaatatgta caggaggcca aaattggccg tgctgttatg gacgcggatg tttttatcag
     1561 tctgacccac tttaagggac acgaggccac cggtttcggc ggcacaatca agaacatcgg
     1621 catgggctgc ggctcccgcg ccggaaagac agagcagcac agcggcggca tggcgaatat
     1681 tgtgccggaa aagtgccgcg gctgccgcaa gtgccaaaag gagtgtgcca acggtggctt
     1741 ggagtttgac gaagtcaaaa agaaaatgac ggtgaatgaa gacaattgcg tcggctgcgg
     1801 gcggtgcctg ggcgcatgca atttcgacgc aatcgagttc aacgattccg ctgccaacgc
     1861 tctgttgaac tgccggatgg cggaatatac caaggcggtg gtggatggcc gccccaactt
     1921 tcatatctcc cttgtggtgg atgtatcccc gaactgtgac tgccatgggg aaaacgacgc
     1981 gcccattttg ccggatatcg gtatgttcgc atcctttgac ccgctggctc tggatcaggc
     2041 ctgtgcggac gcttgcctga aggcacagcc gatgccggga agccagctgt ccgacaatat
     2101 ggcaaaagcg gattttgtcg accaccacga ccacttcacc aactcgacgc ccgaatccga
     2161 atggctctcc tgtctggagc acgcggagaa gatcggtctg ggtacccgga gctatgaact
     2221 gatcccgatg aaataagccg gatgcaatga aaagccgcac cgatatcccc ctcatttgac
     2281 gtacacttgc agcgaaaggt ataataagag atgttgagac tatcatgata ctaaagagga
     2341 taataaagtg ctgctattaa gtgcggaaaa aatcagcaag agctacgtag ataaaatact
     2401 gctgaacgat gtggccttcg gcattaacag tggggaaaaa atcggtttaa tcggaattaa
     2461 cggcgcggga aaatcaactt tgttgaaaat catcgccgga ttggaaactc cggataccgg
     2521 aacggtcacc aaattcggcg gggtgcgcgt gggctatctg ccgcagaatc cgctgtttga
     2581 agacggcatt acggtgcttc agcaggtggt aaagggaata tcggaacagc agcgggaatc
     2641 aaaggaatac gaatgcaagt cgattcttac caggctggga atcaacgaat ttgacaagcc
     2701 actgagccag ctttccggag gacagaaaaa gcgcgtttcc cttgcaagcg cgctggtaac
     2761 gccggtggaa gtgttaattc tggacgaacc aaccaaccac ctggacaacg atatggtcga
     2821 ctggctggaa acctatctcg ccggttacaa cggggcactg ctgatggtga cccatgaccg
     2881 ctattttctg gaccgtgtga ccaataaaat aatcgagctt caaaatggaa atttgtacgc
     2941 ctaccaggcg aattacacga gctatcttga actgaaagcg gaacgcgaaa gcatggcagc
     3001 ggccagcgag cggaaacgcc agaccctgct gaaaaaggaa ctggcgtgga tcagccgggg
     3061 tgcccgcgca cggagtacca agcagaaatt ccgcgtggaa cgttatgaag agatgagcgc
     3121 tcaatcggct gaaccggaac aggaaaatct gcaaatgagc tccatgagct caagacttgg
     3181 gaaaaccatt attgaaatcg gcggcatttc gaaggcttac ggcggcaggc agctgatcct
     3241 gaatttttcc tacaatctgc tgcggcggga ccggatcggc atcatcggcc cgaacggctg
     3301 cggaaaatct actctgctga aaatgatcgt cgggaccgtc tccccggacg aaggggatgt
     3361 ccgaattggg gaaacagtgc ggatcggcta tttctcgcag gaatgcgagg agatggaccc
     3421 aaatcagcgc gtgatcgatt atgtcaaaga gatttcatcg gaggttgtaa cgccgtcggg
     3481 aacactgacg gcctcccaga tgcttgaaac ctttctgttt tcctccgact tgcagtattc
     3541 cctcattaaa aagctttccg gaggagagcg gcgcagactg tatctgctgg ggattttaat
     3601 gcaggctccg aacgttttgc tgttcgacga gcccaccaac gatctggata ttcaaacgct
     3661 gatcattctt gaggattatc ttcagaattt ttcaggagcg gttatcgcgg tatcccatga
     3721 ccgctatttc cttgacaaaa ttgcggagca tgtcttttcg attgaaca
//
LOCUS       DATALY010000288         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_149391,
            whole genome shotgun sequence.
ACCESSION   DATALY010000288 DATALY010000000
VERSION     DATALY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149391"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VHR42_07875"
     CDS             <1..1073
                     /locus_tag="VHR42_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX3027127.1"
                     /translation="FHPEQDGELEKDGLVGLLPYRSYNGNSAVRLGTAFLNRVMGCYE
                     AGQKFVQSPLWEKLERDSLCLNIGGDTYCYDRPTVSYGLNRFAYRHGVKTILWGCSIE
                     KDGIDREMLRDLGRYTLIFPRESITRENLIEAGMEPARLILTSDPAFALGMQPTELPD
                     CFEAGNTVGINLSDLAVGMESDRGIVCANYRAVIRYILNNTDMCVALIPHVYDARTGN
                     GDLLVLKELYQEFESSRRVSLVGEERNCMQLKYIISKCRFFLGARTHSVIAAYSGCVP
                     TLAVGYSVKSQGIARDIFGEYRDLVVSVRQLRAEDELRDRFVRLTERETKIREQYREV
                     LPGYIQRAYAAGRRIRRLWKGE"
     gene            1082..2086
                     /locus_tag="VHR42_07880"
     CDS             1082..2086
                     /locus_tag="VHR42_07880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX3027128.1"
                     /translation="MEKLVSLVTPCYNCEKFLPAYFKSLLSQTYPRLELIFVCDGSTD
                     GTERLIREAIPRFERRNVSAKLICQENRGLGGAVAAGIREVTGDYIAWADPDDILMPD
                     SIAKRADFLSAHPEYGLVRSNGYVYMEGQSTPTGFIVNSKKDKKREEIFEPYVLFQSY
                     FCPGCYMLRTSAFLQVNPDRTIYPSRFGQNIQLVAPVAYRYRCGYLDDPLYCYVVYEN
                     SMSRNGGVQTLERQIAQLDGLEDIDIHVLDQIGGGARSLIPNVKRFFAYRRFYFACRF
                     HDRKKKEEYYRLLRETGRPGLKCAVMSRFEYSGALELLIRFCDRLAPPAGTHRSVRRQ
                     "
     gene            2098..3240
                     /locus_tag="VHR42_07885"
     CDS             2098..3240
                     /locus_tag="VHR42_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX3027129.1"
                     /translation="MNYVIKKNRCTGCGACREVCPRRAVTMIRDSEGFLYPCVESGLC
                     IGCGACLRACPVLSERKVFRPVFYGCKAIDKTISAESSSGGVFPVIAGELLAAGGAVF
                     GAAYEEGEVKHVCVESWSGLSKICGSKYVQSDLSGCLKLTKKMLKSGRKVLFSGTPCQ
                     IAGLKSSLGRDYDGLITVGVICHGVASHKVFSKYLSGLEQSFGGRAVSVCFRDKSTGW
                     DSYSVKIRFDNGTVYQKKASEDPYLQSYLSNLTLRPSCYRCAFLGYHTVSDLTLGDFW
                     NVKNVKPEFYDPEGVSLVGVHTEKGAKLLESVNDRLDSVPVDRPDAEKGCPNLFHAPF
                     CNFSGRGLFRRIDTIPIEENLAQSLTPAFPARLINSAGRHLRIRWR"
BASE COUNT          738 a          844 c          941 g          775 t
ORIGIN      
        1 cttttcatcc ggagcaggac ggggaacttg aaaaggacgg cctcgtcggg ctgctcccct
       61 accgcagtta taacggaaat tccgccgtcc ggctcggcac ggcgttttta aacagagtca
      121 tgggatgtta tgaggccggc cagaagtttg tccagtcgcc tctatgggaa aagcttgagc
      181 gggattcgct ctgcctgaat atcgggggcg acacctactg ctacgaccgg cccaccgttt
      241 cctacgggct caaccggttc gcttaccggc acggcgtgaa aaccattctg tggggatgct
      301 cgattgaaaa agacggcatc gaccgggaaa tgctccggga tcttggccgt tacacgctga
      361 tttttccgcg ggaatccata acacgggaga acctgattga ggccggtatg gaacccgcaa
      421 ggctgattct aacttccgac cctgcctttg cgctcggcat gcagccgacg gaactgccgg
      481 actgttttga agcgggtaat acggttggaa tcaatctcag cgatcttgcc gtcggaatgg
      541 aatccgaccg gggaatcgtc tgcgcaaatt accgcgcggt catccgctat attctgaaca
      601 acacggatat gtgcgtggcc ctgattcctc acgtttacga cgcccggacg ggaaacggcg
      661 atctgcttgt gctcaaagaa ctttatcagg agttcgaatc ctcaagaagg gtcagcctgg
      721 tgggcgaaga acgcaattgt atgcagttaa aatatatcat ctcaaagtgc agattttttt
      781 tgggcgccag aacccattcg gtcattgcag cttattccgg ctgcgttccg accctcgcgg
      841 tgggctattc ggtaaaatcc cagggaattg cacgggatat tttcggagaa taccgggatt
      901 tggtcgtttc cgtccggcag ctgcgggcgg aggacgaact ccgggaccgg ttcgtccgtc
      961 tgaccgagcg ggagacaaaa atccgtgagc agtaccgcga agttttaccg ggctatattc
     1021 agcgggccta cgcggccggc cgccggatca gacggctttg gaaaggagag tgacgacgcc
     1081 tgtggaaaaa cttgtgagtc ttgtgacacc ctgctataac tgtgaaaaat ttctgcccgc
     1141 ttattttaag tctttactca gccagactta tccgcgactg gagctcattt ttgtatgcga
     1201 cggctccacc gacggtaccg agcggctgat ccgcgaggca attccccgtt tcgagcgaag
     1261 gaatgtttcg gcgaaactca tctgtcagga aaaccgcggg cttggcggcg cggttgcggc
     1321 gggaatccgg gaggtcaccg gggattatat cgcctgggcc gatccggacg acatcttgat
     1381 gccggattca atcgccaaaa gagcagattt tttaagcgct catcccgaat acggccttgt
     1441 ccgatcaaac ggttatgttt acatggaggg gcaatcgact ccgaccggtt ttatcgtcaa
     1501 cagcaaaaag gataagaagc gggaagagat ttttgagcct tatgtactct ttcagtcata
     1561 tttctgcccc ggatgttata tgttgagaac gagcgcattt ttgcaggtca accccgaccg
     1621 cacgatttat ccgtcacgat tcgggcagaa tattcagctt gtcgcgccgg tggcctatcg
     1681 ttaccgctgc ggctatcttg acgatccgct ttactgctat gtggtctatg aaaacagcat
     1741 gtcgagaaac ggcggggttc agacgcttga gcgccaaatc gcacagctgg acggccttga
     1801 agatattgac attcatgttc tcgatcagat cggcggcggc gcccggagtc tgattcccaa
     1861 tgtgaaacgt ttttttgcct accgcaggtt ctatttcgcc tgccggttcc acgaccggaa
     1921 gaaaaaggag gaatattacc gtctgcttcg ggaaaccggc agaccgggcc tgaaatgtgc
     1981 cgttatgagc cgctttgagt attccggggc gctcgaactg ctgatccgtt tctgcgaccg
     2041 gttggcgccg ccggccggaa cacatcggtc tgtcaggcgt cagtaaggag ttttaacatg
     2101 aattatgtca taaagaaaaa cagatgcacc ggatgcggag catgccggga agtatgcccg
     2161 cgacgggctg tcacgatgat ccgtgacagc gagggcttcc tttatccctg cgtggaatcg
     2221 gggctttgta tcggatgcgg ggcctgtctg cgcgcctgcc cggtattgtc ggaaagaaag
     2281 gtgtttcgtc ccgtgtttta cggctgcaag gccattgaca agaccatttc cgccgaaagc
     2341 tcctccggcg gcgtgttccc ggtcatcgcg ggagagcttc tcgcagccgg cggcgcggtt
     2401 ttcggcgcgg cttacgaaga aggagaagta aagcatgtct gcgtggaaag ctggagcggc
     2461 ctttcgaaaa tctgcggttc gaaatatgtg cagagtgatt tgagcggctg cctgaagctt
     2521 accaaaaaaa tgctgaaatc cggaagaaaa gtcctttttt ccggaacccc ctgtcagatc
     2581 gccgggctca aatcctcact cgggcgggat tatgacggtc ttatcaccgt cggcgtgatc
     2641 tgtcacggtg tcgcgtcgca caaggtcttt tcaaaatatc tatccggtct tgagcagtct
     2701 tttggcggca gggccgtctc ggtttgtttc cgcgataaat ccaccggttg ggatagctat
     2761 tcggtgaaaa tcaggtttga taatggaacg gtttatcaaa aaaaggcgtc ggaggacccc
     2821 tatctccaaa gctatctctc caacctgaca ttaaggccct cctgctaccg gtgtgcattc
     2881 ctcggttatc acacggtatc cgatctgact ttgggggatt tttggaacgt caaaaatgtg
     2941 aagccggaat tttatgaccc ggaaggtgtc tcgctcgtcg gagttcacac ggaaaaaggg
     3001 gcaaagcttc tcgaaagtgt gaacgaccgg ctggactccg ttccggttga ccgaccggac
     3061 gcggaaaaag gctgcccgaa tctgtttcac gctcccttct gcaacttttc cggcagaggg
     3121 ctgttccgac gtatcgacac gataccgata gaggaaaatc tggctcagag ccttaccccc
     3181 gcttttcccg cccggctgat caattctgcg ggaagacatc tgagaatccg gtggcgctga
     3241 gcatgacggt cgcttaaggg aattgaatcg gaggataaaa tggaaaaaag caagcgtg
//
LOCUS       DATALY010000289         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_494613,
            whole genome shotgun sequence.
ACCESSION   DATALY010000289 DATALY010000000
VERSION     DATALY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494613"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..906)
                     /locus_tag="VHR42_07890"
     CDS             complement(7..906)
                     /locus_tag="VHR42_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3027130.1"
                     /translation="MEKLKKRRFLFSAVRLAVQIVCFILLPTVYIGALDGILAIYLAT
                     IQQNFSAALLPQLIEVLATVPVTFFLGRFFCGWMCAFGSYTDFVYRISQKLFHKKLKL
                     SERMDRWMKLIKYAILLILAITVWTLGKTAFLSVSPWDAFGMLATVGKVPNLAYVASS
                     MTAGLILLLIVTALSAVTERFFCRYLCPMGAVFSLTSLLRVARIKKPSAGCGKCRICT
                     ESCAMGIPLYQIDTVRSGECIHCMKCIAACPRNNARLSVTGSDMRPLVASTVAAAAMT
                     GFTYLGNFSFNHRSMRSLSFSFL"
     gene            complement(911..1879)
                     /locus_tag="VHR42_07895"
     CDS             complement(911..1879)
                     /locus_tag="VHR42_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX3027131.1"
                     /translation="MISAFHKKKSAEKTFFALGTVNAVKIYGSKDVSAVDAAIARIRE
                     IEDRMSAFLPDSDLAVLRKNAGRGPTEIHRDTFRVLQKAASFGALTDGAFDVTVRPLT
                     ELWGINKKDSFIPSEEELRKARSLVNYRDLQLDQNLWSASLARRGQALDLGSIAKGFA
                     ADEAGQVLLGRGIECAVINLGGNVLTLGKRPDGAPWRIGVQNPLAPSGQPIGVLSVDG
                     GTSVVTSGSNERFFIRDGKRYHHLLNPRTGEPAQNSMLSVTVLCPCSADADAATTALF
                     LLGPEKAKSILRKIRAEAVIIGRDLSVTVSDGLLNRFQPIYETVKR"
     gene            2162..2704
                     /locus_tag="VHR42_07900"
     CDS             2162..2704
                     /locus_tag="VHR42_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEX3027132.1"
                     /translation="MFWIDRTLYQGKPQNERLPKEAAVYNLLDSLEIPYFRMDHDATA
                     HIEGCEEVGKILGVEICKNLFLCNRQKTDFYLLAMPGGKQFRTKDLSAQIESSRLSFA
                     DAEYMEKFLNVTPGSVSVMGLMNDRENRVKLMLERDILQSEYFGCHPCINTSSLKLKT
                     SDVLDKFLPHVGHSPTFVTL"
BASE COUNT          711 a          685 c          699 g          657 t
ORIGIN      
        1 attatttcac aaaaaactaa aactaagcga acgcatggac cggtggttga agctgaaatt
       61 tcccagataa gtaaagcctg tcattgcagc cgccgcgaca gtgctggcta caaggggacg
      121 catgtcgctg ccggtgacgg aaagccgggc gttgttccta gggcaggcag cgatgcattt
      181 catgcagtga atgcattcgc cgcttcgaac ggtatcgatc tggtaaagcg gaattcccat
      241 cgcgcagctt tcagtgcaga ttctgcactt gccgcatccg gcagatggct ttttaatcct
      301 tgcaacgcga agcagggaag taagggagaa aacagcgccc attgggcaga gataacggca
      361 gaagaaccgc tcggttacag ccgagagtgc ggtgacaatc aaaagcagaa tcagccccgc
      421 cgtcatactg gaagccacat aggcgagatt tggcaccttc ccgaccgttg caagcatacc
      481 gaaggcatcc caagggctga cggaaagaaa agcggttttt ccgagggtcc acaccgtgat
      541 tgccagaatc aacaggatcg cgtattttat cagcttcatc caccggtcca tgcgttcgct
      601 tagttttagt tttttgtgaa ataatttttg tgagatccgg tagacaaaat ccgtatagga
      661 accgaaggca cacatccagc cgcagaagaa ccggcctaag aaaaatgtaa cgggcacagt
      721 cgccagcacc tcaataagct gagggagaag cgctgcggaa aaattctgct gaattgtggc
      781 aaggtaaatg gccaggattc cgtccagcgc cccgatatac accgtcggaa gaagaataaa
      841 gcacacaatt tgaacggcga gccggactgc gctaaacaga aatcttcgtt ttttcagctt
      901 ttccattgct tcacctcttt accgtttcat aaatcggctg aaaccgattc agcagtccgt
      961 cgctgaccgt aacggacaga tcacgcccga taatgaccgc ttccgcgcga atctttcgga
     1021 gaatcgattt cgccttttct gggccaagca ggaaaagggc ggttgttgcg gcatccgcat
     1081 ccgcggagca gggacacagt accgttacgc tcagcataga attctgggca ggctctccgg
     1141 tccttgggtt aagcagatgg tgataccgtt ttccgtcccg gataaagaaa cgctcgttgc
     1201 tgccggatgt aacaacggag gttccgccgt ccacggacag aaccccgatc ggctgcccgg
     1261 aaggcgccag cggattctga actccgattc gccaaggcgc tccgtccggc cttttcccaa
     1321 gcgtcagcac attgccgccg agattgataa cggcgcattc gattcctctg ccgagcaaga
     1381 cttgcccggc ttcgtctgca gcaaaccctt ttgcaatgga accgaggtcc agtgcctgcc
     1441 cacggcgcgc cagtgaggcg ctccagagat tctggtcaag ctgaaggtca cggtaattca
     1501 cgagggacct ggcctttcgg agctcctctt cggaagggat gaaggaatct tttttattga
     1561 ttccccaaag ctcggtcaga ggacgaactg tcacgtcgaa ggctccatcc gtcagagccc
     1621 cgaaggacgc agccttctga agcacccgga aggtatcccg atgtatttcc gtcggtccgc
     1681 gtcccgcgtt ttttcgcaaa acggcaaggt cgctgtctgg aagaaaggcg gacatccgat
     1741 cttcaatttc acggattcgt gcaattgcgg catccacggc ggaaacgtct ttgcttccat
     1801 atattttaac ggcgttgacc gttccgagag cgaaaaaagt tttttcggca gatttctttt
     1861 tatgaaatgc ggagatcatg ttttttcctc caatcatctg aaactgacat cagtataaaa
     1921 atttgatatt aatgtggagt gaaaaaaaca aaaaggatcg atgaatcgtt cgtgaatttt
     1981 ctttgaagcc gtctgccact gaaacccgtt gtaatctatt tcgtcagtcg acagcttttg
     2041 ctcgtttttt gtctcacaga gtttagaatt gtcggaattt ctggaagaaa aacccgcttt
     2101 cttgcttcat aaaatggaat gtgttatgat aggggaaaat ttgctgcaac gggatgatga
     2161 aatgttctgg attgatcgga ctctatatca agggaaaccg cagaatgagc gcctgccaaa
     2221 ggaagcagcg gtttacaacc tgctggattc gcttgaaatc ccatatttca ggatggatca
     2281 tgacgcaact gcacacattg agggctgcga agaagttgga aaaatcctcg gggtcgaaat
     2341 ctgcaagaat ctgtttctgt gcaaccggca aaaaaccgac ttttatctgc ttgcgatgcc
     2401 agggggaaag cagttccgca cgaaggacct ttccgcacag atcgaaagtt cccggctctc
     2461 cttcgcagat gcggagtaca tggaaaaatt tctgaatgtg acgcccggct ccgtcagcgt
     2521 catgggtctt atgaacgata gagaaaaccg cgtgaagctt atgcttgaac gcgatattct
     2581 gcaatcggaa tattttgggt gccatccctg catcaacacg tccagtctga aacttaagac
     2641 ttccgatgtc ttagataaat ttctccctca tgtaggccat agcccgacct ttgttacact
     2701 gtgaaaattt gagaatacct gacaggatgc tgtcattttc cggtgctatc at
//
LOCUS       DATALY010000290         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_324725,
            whole genome shotgun sequence.
ACCESSION   DATALY010000290 DATALY010000000
VERSION     DATALY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324725"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1026)
                     /locus_tag="VHR42_07905"
     CDS             complement(301..1026)
                     /locus_tag="VHR42_07905"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEX3027133.1"
                     /translation="MSVEDFKKLVTESKNIVFFGGAGVSTESNIPDFRSETGIYSGSG
                     FRFAPEVMLSHEFFYTHPKEFYQFYFEKLVYPDAEPNAAHLALAELERQGILRAVITQ
                     NVDGLHQKAGSRIVLELHGSALRNTCTRCGKKVNLDFMLRFRPGVPTCECGGIVRPDI
                     VLYGEGLDADVVDASVRAISLADLLIVGGTSLVVYPAAGLIRYFRGRNLVLINRDRTD
                     YDDSAALVFHDSIGKILSQVVAK"
     gene            1244..1831
                     /locus_tag="VHR42_07910"
     CDS             1244..1831
                     /locus_tag="VHR42_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3027134.1"
                     /translation="MSDKADSNKMVKENALINAAFELFSKSGINGTAIDEIVKKAGVA
                     KGTFYLYFKDKYDILDRIVIKKSAMLIQNALTEMSGRKFDSGTDKILFFINYIIDTLT
                     QSRILLKILYKNLSLGLYRRVMDDPVRGAEFRQIVGEFNRLREGGSKEENGYLLFILL
                     EMTGSVCYSAILYGEPAPIDQMKPILLKTVRKILS"
     gene            1865..>2865
                     /locus_tag="VHR42_07915"
     CDS             1865..>2865
                     /locus_tag="VHR42_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3027135.1"
                     /translation="MRKYGLFISRHRVAVLVIAILLLIPALFGYIKTRINYDILSYLP
                     AGIDSVDGETVLDKTFSDAATGMLVIEGMPDRDISSVKEQIKTVPGVENVIWKDDITD
                     ITVPQSMLPKNMADVFQKGNASLLLVQFKNGSSSLETQNAIAKIRSRLNRQCFLSGMS
                     SIVKDTVDLTEQETPKYILLAVVLSLIFMSLTINSWLVPFLFLGSIGMAILYNLGSNV
                     IFGEISFITKALAAVLQIGVTMDFAIFLYNRYEHELKKNPDREQAMAGAIANSFTAVF
                     GSGLATTCGFLALGIMRLGLGKDIGFVMAKGVFLSVISTFTILPSLLLVFNGPIHKHT
                     "
BASE COUNT          733 a          761 c          706 g          665 t
ORIGIN      
        1 ctgcgcggag aatctgcacg cggcggtggt cgatcgcatc aacgccgtaa cggccgcgcc
       61 gcagacgcag tcggaaagca aaccttcatc cgggatgccg cagccaagcg gtaatggcta
      121 ttgtgacacg attacgtatg ccgtataatg ccgacccatt tgaataagtg attcatagct
      181 tttcaaatga gtaaaacact atacaggcgg ttgataaaag aaaagagcaa aaatataacg
      241 ccatgccgat accggaaacg gtaccgagca tggcgaattt cttatattga tcataaaaag
      301 ttatttggca accacttgcg agaggatttt tccgatgctg tcgtgaaaga ccagcgcagc
      361 cgaatcatca taatcggttc gatcgcggtt gatcaggaca aggttccggc ccctgaaata
      421 acggatgagg cccgcggcag ggtagacgac cagcgacgtg ccgccgacaa tcagcaagtc
      481 agccagtgaa atggcgcgga cggatgcatc gaccacatcg gcgtcaagcc cttcgccgta
      541 aagcacgata tcaggccgca caatgccgcc gcattcgcag gtgggaacgc cggggcgaaa
      601 ccgcagcata aaatcaagat tgaccttttt cccgcaccgg gtgcaggtat tgcgcagcgc
      661 ggagccgtga agctccaata caatgcggct gcccgccttc tgatgaaggc cgtcgacgtt
      721 ctgggtaatc accgcgcgga gtatgccttg ccgctcaagc tccgcgagcg caaggtgcgc
      781 ggcattcggc tcggcgtcgg ggtagacgag cttttcaaaa taaaactggt agaattcctt
      841 cggatgggta taaaaaaact catggctcag catcacttcc ggcgcaaagc ggaagccgga
      901 cccggaataa atacccgttt cgctgcggaa atccgggata ttgctttcgg tggaaacccc
      961 cgccccgccg aagaagacga tatttttact ttccgtcacc agttttttaa aatcctcgac
     1021 gctcattttc cgcagaatcc tttcccttca tttccggtat attataccac cgttttggct
     1081 gggataccag aaggacgcgc tggaaccggc aaactgtata aagagcggtg tgatttccct
     1141 gcaattttaa tggatcttaa tattgactat cggtcattta tctattataa tcttactata
     1201 aaaatgacta tgggtcattt ttatcaaaag cagggaggaa gttatgagcg ataaagcgga
     1261 cagcaacaaa atggtgaagg aaaacgccct gattaacgca gcttttgagc ttttttcaaa
     1321 atccggcatc aatggaacgg cgatcgacga gattgtcaaa aaggccggcg tcgcgaaagg
     1381 cactttttat ctctatttta aggataagta tgacatttta gaccgcattg taatcaaaaa
     1441 atccgcaatg ctcatccaga atgcccttac ggaaatgagc ggcagaaaat tcgacagcgg
     1501 cacggataaa atccttttct ttatcaatta catcatcgat accctgaccc agtcgcggat
     1561 tctgcttaaa attttatata aaaatctgtc gctgggcctt taccgccgcg tgatggacga
     1621 ccccgtaagg ggcgcggaat tccggcagat cgtcggtgaa ttcaacaggc tgagggaagg
     1681 gggcagcaaa gaggaaaacg gctatctttt gtttatcctt cttgaaatga ccgggtctgt
     1741 ctgctacagc gcaattcttt acggcgagcc ggctcccatt gaccagatga agccgatttt
     1801 gctcaaaaca gtcagaaaga tcctgtcata acaattttca tgcaacagaa agaagatgac
     1861 atgtttgcga aaatacggac tttttatctc aaggcacagg gtggcggtgc tcgttatcgc
     1921 gattctgctg ctgattcccg cattattcgg ctacataaag acgcgcatca actacgacat
     1981 cctctcctac ctgccggccg gcattgattc cgtagacgga gaaaccgtgc tcgacaaaac
     2041 cttctccgac gcggctaccg gcatgctggt gatcgaaggc atgcccgacc gggatatttc
     2101 ctccgtcaag gaacagatca agacagtccc cggtgttgaa aacgtgatct ggaaggacga
     2161 cattaccgat atcaccgtcc ctcaaagcat gcttcccaaa aacatggccg acgttttcca
     2221 gaaggggaac gcttcactgc tgctcgtcca gttcaaaaac ggctcctcct ccctcgaaac
     2281 ccagaacgca atcgcaaaaa ttcgctccag gctaaaccgc cagtgtttct taagcggcat
     2341 gtcctccatt gtcaaggaca cggtcgatct gaccgagcag gagacgccca aatacatcct
     2401 gcttgccgtt gttctctccc tgatcttcat gtcgctgacc atcaactcct ggctcgttcc
     2461 ctttttgttc ctcggctcaa tcggcatggc gatcctctac aatctagggt caaatgtgat
     2521 cttcggcgag atttccttca tcacaaaggc gcttgccgcg gtgctgcaga tcggcgtcac
     2581 gatggatttc gccattttcc tttacaaccg atacgagcat gagctcaaga agaatcccga
     2641 ccgggagcag gcgatggcgg gggccatcgc gaattccttc acagccgtgt tcggctccgg
     2701 gcttgcaacg acctgcggct tcctcgcgct gggcatcatg cggctcgggc tcggcaagga
     2761 catcggcttt gtcatggcga aaggtgtttt cctcagcgtc atctcgactt tcaccattct
     2821 cccttccctg ctgctggtct tcaacgggcc gattcacaag cacac
//
LOCUS       DATALY010000291         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_579878,
            whole genome shotgun sequence.
ACCESSION   DATALY010000291 DATALY010000000
VERSION     DATALY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579878"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VHR42_07920"
     CDS             <1..830
                     /locus_tag="VHR42_07920"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEX3027136.1"
                     /translation="LRNTFTRKSSITVMGKEISQIRGKDLRVFQKRIGMIFQNFNLLK
                     RANVYDNVALPLQFWGENKNSEKNRAKIRNLLQLVGLTDKIHSKPAELSGGQKQRVAI
                     ARALVMDPAILLCDEATSALDPQTTKEILALLSKINREMGIIVILVTHQMEVVKQICN
                     RVAFIKDGGIVAEGKPEDLFVVPNQDIKQFLGGTPDVLPKSDVNVQLFFTGDYAGDPV
                     ITQLSRSLDIDFSIVWGKLENFRSDVMGSLVINIRENDQERVFDYLKQHGVLWEVLK"
     gene            832..1467
                     /locus_tag="VHR42_07925"
     CDS             832..1467
                     /locus_tag="VHR42_07925"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEX3027137.1"
                     /translation="MADTIIPALQETAIMVGASTLLSVILGFIPAIILTVTAKDGLRP
                     NRVVYGVLDFLVNVFRSFPFIILMIVIIPFTRMVAGRAIGTTAAIVPLTVSAIPFVAR
                     IMESALRAVDPGVIEAARAFGASDTQIIFRVSIKEAIPSILAGITLTVINLVGYSAMG
                     GAIGAGGLGDVAIRYGYQQYRMDYLIVTCIVLIVFVQAVQLLGNFLFEKLS"
     gene            complement(1603..2427)
                     /locus_tag="VHR42_07930"
     CDS             complement(1603..2427)
                     /locus_tag="VHR42_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3027138.1"
                     /translation="MEAMEHLTFQNLGCTVHYWYRKGSENKWVFFFHGAGVDHAMFEA
                     QYGLFDETYHILTWDARGHGLSKLEPGKKFVFVDMISDCKKLFERYSVKRATLIGQSM
                     GGNLAQEIAYQSPELVDRMVLIDCAKNTGKLTGSEKLALKSARFLFALYPWKTLIRQS
                     AEACANTESVQKYIYDCFNRLDKQTFVTVILNMAGSCLHEDAAYRFRQRVLLLCGVDD
                     QLGNIRKVAGPWAEEDGNCTLHMVEHASHNSNQDNPEQVNQWISDFLELQKVQPLS"
     gene            complement(2509..>3035)
                     /locus_tag="VHR42_07935"
     CDS             complement(2509..>3035)
                     /locus_tag="VHR42_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3027139.1"
                     /translation="AQKHVQTRKAYRNISTTALIGWINHLAGMGEFTLGSNQAHLLEP
                     VFEASEEFDYLPIWVHAFFPFTLQDIRDTADMAKKHPRTPVILGHLGGENWMDALDLA
                     GEIPNLYLDTSAYYSTYVLGIVINELPQKCIFGVDRPFGDLELSKEAILKLAKTPAVS
                     DAVLGENMARLLNL"
BASE COUNT          720 a          770 c          764 g          781 t
ORIGIN      
        1 aacttcggaa cacatttacc agaaaatcca gcatcacagt tatggggaag gaaatttcgc
       61 agattcgtgg gaaggacctg cgggttttcc agaaacgcat cggcatgatc tttcagaatt
      121 tcaaccttct caagcgcgcc aacgtctatg ataacgtcgc tctgccttta cagttctggg
      181 gcgaaaacaa aaacagtgag aaaaacaggg cgaaaatcag gaacctgctg cagcttgtgg
      241 gcctgacgga taaaatccac agcaaacccg ccgaactgag cggcggtcag aagcagcgcg
      301 tcgccatcgc gcgcgctctg gttatggacc ctgcgattct gctttgcgac gaagcaacct
      361 ctgctctgga cccccagaca accaaggaaa ttctcgcttt gctttccaaa atcaaccggg
      421 agatggggat tatcgttatc ctcgtgaccc atcagatgga agtggtaaag cagatttgta
      481 atcgggttgc gttcattaaa gacggcggaa ttgtcgcgga gggtaaaccg gaggacctgt
      541 ttgtggtgcc gaatcaggat atcaagcagt ttctgggcgg aacgccggac gttctcccga
      601 agtcggacgt aaacgtgcag ctctttttta ccggcgatta cgcgggtgac cccgtaatca
      661 cacagctttc ccgcagcctt gacatcgact tttccatcgt gtggggtaag ttggaaaact
      721 tccgcagcga cgttatggga agtcttgtca tcaacatacg ggaaaacgat caggagcggg
      781 ttttcgatta cctgaaacag cacggagtcc tatgggaggt attaaagtaa catggcagat
      841 acaattattc ctgcattgca ggaaacggcg atcatggtgg gggcctcaac gttgctttcc
      901 gtcattctgg ggttcatccc tgccattatt ctgacggtta ccgccaagga cggtctgcgt
      961 cccaaccggg tcgtatacgg tgtgctggat tttctggtaa atgtgttccg aagttttccc
     1021 tttattattt taatgatcgt catcatccca tttaccagaa tggttgcggg cagagcgatc
     1081 gggacgactg ccgcgattgt tccgctgacg gtttccgcca ttccgtttgt ggcgcgtatt
     1141 atggaatcgg ccctgcgtgc ggtcgacccc ggtgtgattg aagcggcaag agccttcggc
     1201 gcatcggata cccaaattat ctttcgggtt tccatcaaag aagcgattcc atctatcctg
     1261 gcgggaatca ctttgaccgt tatcaatctg gttggctact cggcaatggg cggcgccatc
     1321 ggtgccggcg ggcttggcga cgttgccata cggtacgggt accagcagta ccgtatggat
     1381 tacctcattg tcacctgtat cgtgctcatc gtgttcgtac aggcagttca gcttctggga
     1441 aattttctgt tcgaaaagct gtcgtaaaac agcgcatatc aaaacagcag ttaaggtgaa
     1501 ataccttagc tgctgttttt atatattgag caattgctca tttattttga ctttgcgata
     1561 aagcatcaag aaaatcattc gagccggaat gaacttcgtt ttttatgaaa ggggctgtac
     1621 tttttggagc tccaaaaagt ctgaaatcca ctggttcacc tgctcgggat tgtcctgatt
     1681 cgagttgtgg ctggcatgct ccaccatatg taacgtgcag ttaccgtctt cctccgccca
     1741 cggacccgct acctttcgaa tattgcccaa ttggtcgtct acgccgcaaa gcagcagcac
     1801 ccgctgacgg aaccgatacg cagcgtcttc atgaaggcag cttccggcca tattcagtat
     1861 cacagtaacg aaggtttgtt tatccaaccg attaaagcag tcgtagatat atttctgtac
     1921 gctttccgtg tttgcacatg cttctgcgct ttggcgaatc agggttttcc acgggtagag
     1981 ggcaaataga aaacgggccg atttcagcgc cagtttttcc gaaccggtca gcttccccgt
     2041 gttttttgcg cagtcgatca gcaccattcg gtcgacgagc tcgggggact ggtatgcgat
     2101 ctcctgtgca aggtttccgc ccattgattg gccgatcagg gttgcccgtt taacggaata
     2161 tcgttcaaac agctttttgc agtcgctgat catgtcaacg aacacaaact tttttcccgg
     2221 ttccagtttg gagagcccgt gcccgcgggc atcccacgta agaatgtgat aggtctcatc
     2281 aaacaacccg tactgggctt caaacatggc gtgatccacg cccgccccgt gaaaaaagaa
     2341 tacccattta ttttcggagc ctttccggta ccagtaatga acggtacagc caagattctg
     2401 aaacgtcaga tgctccattg cttccatcga agcgccctct ctctgcttaa ttaatccgat
     2461 tatagacccg ccgagagatt tttgaaagag caaattctga aaagcggatt acaggttgag
     2521 caaccgcgcc atgttttccc ccagaacggc atccgataca gcaggcgttt tcgcaagttt
     2581 gaggattgcc tctttggaga gttccaggtc tccgaaaggc cggtcaaccc cgaaaataca
     2641 tttttgaggc aattcgttga taacaatccc caaaacatac gtggaataat aggcggaggt
     2701 gtcgaggtac agattcggga tttccccggc aaggtccagt gcgtccatcc agttttcgcc
     2761 gccgaggtgc cccagaataa ccggtgtgcg cggatgcttt tttgccatat ccgccgtatc
     2821 ccgtatgtcc tgcagggtaa acgggaaaaa ggcgtggacc cagatcggca gataatcaaa
     2881 ctcttccgat gcttcaaata cgggctccaa cagatgcgcc tgattgctgc ccagcgtgaa
     2941 ttcccccata cccgccagat ggtttatcca accgattaaa gcagtcgtag atatatttct
     3001 gtacgctttc cgtgtttgca catgcttctg cgctt
//
LOCUS       DATALY010000292         7932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_751456,
            whole genome shotgun sequence.
ACCESSION   DATALY010000292 DATALY010000000
VERSION     DATALY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751456"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VHR42_07940"
     CDS             complement(<1..267)
                     /locus_tag="VHR42_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027140.1"
                     /translation="MDQTRSNAIILKKKITARPIYNHTALIDISIAYPQVVIKSNPPV
                     SSKISDFYRQTALDYYNYAMDKLYHTALDDYHERKNNKIPFHTYT"
     gene            442..1833
                     /locus_tag="VHR42_07945"
     CDS             442..1833
                     /locus_tag="VHR42_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3027141.1"
                     /translation="MQLSLLHQSTAKRREMILRGPITPTLLLLSVPTIMMGIVQSAIP
                     VIDGLYINNFSGTVAASAVTYCGPIVMMMSALAQGLSVAGMAIIGQMNGKGEISAAKH
                     VATQIVVSTFLIGLLMMPFLAILSFPISRHVTPSISHDVFLYLSLNSLVLPFSFLEFV
                     YNAIKNSNGKPEAPFIRMVLMLGLKLLFNAIFIAVIPLGIVGTVISSLLANMLICVWM
                     YFEMFVLKGDDKLELRGFRFDFEVICELFKIGVPSMLSSVMLNLGFFLINNEVEKYGP
                     VTLNGQGIANNITSICFIVPSSFGSSVTTMVSMNVGAEQGNKARRSCIAGCIVSAISA
                     VLLIALVVPLSPHITVLFTRNPDVLHIANKALHIYTYSVVGFGICMVQLGAFIGLGRT
                     SIPLVIGVLRIWLLRYMFILATEQFLGVYSVFWGNLFSNYMCAVITTVLFLKIHWVSV
                     IKTDTKDKAAAAV"
     gene            complement(1951..2139)
                     /locus_tag="VHR42_07950"
     CDS             complement(1951..2139)
                     /locus_tag="VHR42_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX3027142.1"
                     /translation="MASLNNGPDKGKKSVLLRLSNESWNEIAAWAEEEFRSINGQIEY
                     LLNEAIHQRKKSQKKDLR"
     gene            complement(2143..3051)
                     /locus_tag="VHR42_07955"
     CDS             complement(2143..3051)
                     /locus_tag="VHR42_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3027143.1"
                     /translation="MSTDHAKEKNIRVSTGWIYLFWTFLFLILSALSFVGLGVFADNP
                     KMNAAVPLLIVAGVLFAILFFLFMSGFFNLAPNQAAVLSLFGDYRGSVRQAGILWVNP
                     FYSKKKISLRSRNLNGEKLKVNDQIGNPIEIAAVIVWKVNDTFRASFDVENYDSFVKV
                     QSESAVRHLANMYPYDFSEGESKTSLRGNTEEVAETLKQELQERVAKAGVEIEEARIS
                     HLAYAPEIAAAMLQRQQASAVIAARQMIVEGAVGMVEMAMEKLNEKGVVELDEERKAA
                     MVSNLMVVLCAEKAASPIINAGTLYH"
     gene            3266..3913
                     /locus_tag="VHR42_07960"
     CDS             3266..3913
                     /locus_tag="VHR42_07960"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX3027144.1"
                     /translation="MSEFITNIAYHINLFPLWAVFLFVFLSACIQQFFPPYPSEILLL
                     FLGCLAASRLLPGVPVIAIYAFGTVLSSLFVFHLSRRGGKQLLKNKFVMKFFPRKYQL
                     KAGVYVRRYGILALIFCKFLPGVNTLSLIMGGIMGLKGPAAWLSISITGIVANVVYFL
                     AGTIIGNNIPALYHFSKNFSIGAMIAVAVIIAAAAAFYLVGKYMAGRKKKPAASE"
     gene            4024..4704
                     /locus_tag="VHR42_07965"
     CDS             4024..4704
                     /locus_tag="VHR42_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEX3027145.1"
                     /translation="MTKIRFFSAVLIGWLLICPVQAASSLKVYGFSTEFKEPSLQYSC
                     SYPRITGIDNNQMQQRVNVMIREKAETSVQTAQYISKKLNGAVKVSGKFHYQLKRNEN
                     GILSLVLQDSLFTGNERGIQQQTGVTLNTVNGRIYKLRSLFVEHADYVGVISDEIHRQ
                     ISQKGLKNQQLESFKKIRNDENFYLTSDSLVVFFDQYQYGNSDIREFSIPLNSLNGML
                     KPEFRLLT"
     gene            complement(4798..5070)
                     /locus_tag="VHR42_07970"
     CDS             complement(4798..5070)
                     /locus_tag="VHR42_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027146.1"
                     /translation="MNDAGKTIRLDYLFNFLLLLSFAVVLILGYLVQNWHHSLGIADR
                     VILAVFCLFNGSWWFLVKKEHLKGTILDLVGLFALVTALMGRGFNS"
     gene            complement(5094..>7932)
                     /locus_tag="VHR42_07975"
     CDS             complement(5094..>7932)
                     /locus_tag="VHR42_07975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX3027147.1"
                     /translation="NACSVKIKADVDGNMEDWLLMFKNETHNHPTEIEPFGGAATCLG
                     GAIRDPLSGRSYVYQAMRVTGAADPRLPVEKTMKGKLPQRKISVGAAHGYSSYGNQIG
                     LATGQVSEVYHPGYVAKRLETGAVIGAAPAENVVREIPEPGDIVVLLGGRTGRDGCGG
                     ATGSSKSHTVESLESCGAEVQKGNAPEERKIQRLFRHPTVTTMIKRCNDFGAGGVSVA
                     IGELADGLKIDLNAVPRKYDGLDGTELAISESQERMAVVVREKDTEKFIELAAKENLE
                     ATAVAVVTAEPRLRMYWNGSEIVNISRDFLNSNGAEKHTGIKVHDCDVASLYLSQPFT
                     DLEQRWKEMLSDLNVCSQKGLSDRFDSSIGASTVLMPFGGEHQSTPIQAMAAKLPVLK
                     GSTTTCSLMAWGFNPVLSSASPYHGAAVAVVESVARIVAAGGSRKKAWLSLQEFFEST
                     KNDPSRWGKPFAALLGAYEAQTGLGIAAIGGKDSMSGSFEEIDVPPTLISFACSVTNA
                     QKVISPEFKTEGDKILLLRPKYLKNGMPDFGSVRRLFDRVEKLISQRKIKAAWALSSA
                     GPAEGITKMAFGNRIGFQSVKNLDRNLLFDQCYGGFLIEVDRKALIRGTLLGHTISQP
                     QIEIGGITMNLDDLEKSWEAPLEEIFPTNAKAQSDSAHTFNYNLRGNARPKAHVTSPR
                     VLIPVFPGTNCEYDTARQFEQAGAVCDTFVIRNLTALAVEDSVKEFEKHIRNSQIIMI
                     PGGFSGGDEPDGSGKFITAFFRNPRVKDAVADLLQNRDGLMLGICNGFQALIKLGLVP
                     YGEIKEMDRDCPTLTFNSIGRHQSTMVRTKVVSVKSPWFMYTNPGDVYTIPVSHGEGR
                     FVASSELIEKLAQNGQIATQYVDLQGRPSMDIHYNPNASVEAIEAITSPDGRILGKMA
                     HSERIGKYVAKNIGGNKDQRIFSAGVDYFAI"
BASE COUNT         1841 a         1878 c         2061 g         2152 t
ORIGIN      
        1 ggtgtatgta tggaagggaa tcttattgtt tttcctttcg tgataatcat ccagcgcggt
       61 gtgataaagc ttatccatgg cgtagttgta ataatccagc gctgtttgcc gataaaaatc
      121 gctgatttta ctggataccg gcggattgct ttttatgact acctgggggt acgcaattga
      181 gatgtcgata agggccgtgt gattatagat gggtcgggcg gtaatctttt ttttcagaat
      241 gatcgcattg gaccgggtct gatccatagt tggcctccgt tctggttccc ggtaatttcc
      301 ggagccgatt aaataaaaaa gcctctatta ttttatgaat ctgcgctcaa attgttcgac
      361 cggattttct tgactcggaa gactgcgagt gataatatat tatcatctta aaaataagtt
      421 tgaaaagtgg cgatctcatc catgcaactt tcattgctcc accaaagtac cgccaaacgc
      481 agagaaatga ttttaagagg gcccatcaca ccaacacttc tcctgctttc cgtcccgacc
      541 attatgatgg gtatcgtcca gtcggcaatt ccggtgatcg acggtcttta catcaacaat
      601 ttttctggaa cagtcgcggc gagcgccgtt acctactgcg gccccattgt tatgatgatg
      661 agcgcgcttg cccagggact tagcgttgcc ggaatggcca ttatcggcca gatgaacggc
      721 aaaggagaaa tttccgcggc aaagcatgtg gcaacgcaga ttgtggtgtc aacttttttg
      781 attggtcttc tgatgatgcc gtttcttgcg atcctgtcgt ttccaatctc gcgtcatgtg
      841 acaccaagca tctcacatga tgtgttcctt tacctcagtc tgaattccct tgtgctgccg
      901 ttctcatttc tggaatttgt ctacaacgca atcaaaaatt caaacggaaa gcccgaagct
      961 ccattcatcc gaatggtatt gatgctcggg cttaaattat tattcaatgc aattttcatt
     1021 gcggtgattc ctttggggat tgtcggcacg gttatttcgt cgctgcttgc caatatgctg
     1081 atttgtgtct ggatgtattt tgaaatgttt gtgctgaaag gcgacgataa gttggaactc
     1141 cgcggctttc ggttcgactt cgaggtgatt tgcgagctgt ttaaaatcgg cgtgccgtca
     1201 atgctctcaa gcgtgatgct gaatctggga tttttcctga ttaacaacga ggttgaaaaa
     1261 tacgggccgg tcacgttaaa cggtcaggga atcgccaaca atattacatc aatctgcttt
     1321 atcgtcccgt cgtcgttcgg ttcgtccgtc accaccatgg tcagtatgaa tgtcggtgcc
     1381 gagcagggga acaaggcaag aagatcctgc attgcggggt gtatcgtcag cgccatatcg
     1441 gctgtgctgc tgattgcatt ggtcgtacct ctttccccgc acattacggt tctgttcacg
     1501 cgtaatccgg atgtccttca tattgcaaac aaggcgctgc atatctacac ttattcggtg
     1561 gttgggtttg gaatctgtat ggttcagctc ggtgccttta tcggactggg aaggacctcg
     1621 attcccctcg tgatcggggt gctgcgtatc tggctgctgc gttatatgtt cattctcgca
     1681 accgaacagt ttctcggcgt ttattcggtg ttttggggca acctgttctc taattatatg
     1741 tgcgccgtga ttaccacggt tttgtttctg aaaatccatt gggtttcggt gatcaaaacc
     1801 gatacaaaag acaaggcggc tgcggctgtc tgatttttcg gaaggttttt taagcgggag
     1861 cggtttcgat caatagatga ttaagagaac aaacaggggc ggaagtttga aacttccgcc
     1921 cctgtgcaat gtatcttgat tgatccgccg ttaccggagg tcctttttct gtgatttttt
     1981 cctttgatgg atggcttcat tgagcaaata ctcaatctgg ccgttaatgg aacgaaattc
     2041 ttcttccgcc caggcggcaa tttcgttcca gctttcattg ctcagccgca gcaatactga
     2101 ctttttcccc ttatcgggcc cgttatttaa cgatgccatg gcttaatgat agagcgttcc
     2161 ggcgtttata attggcgaag cggctttttc agcgcagaga acgaccatca gattagagac
     2221 cattgcggct ttgcgttctt cgtcaagctc aacgacgcct ttttcattta atttttccat
     2281 tgccatttcc accattccca ccgcgccttc gacaatcatc tgtcgggcgg cgatgacggc
     2341 ggacgcctgt tggcgctgga gcatggcggc agcaatttca ggagcatagg caaggtgcga
     2401 gatgcgcgct tcctcaatct cgacgccggc ctttgctacg cgctcctgaa gctcctgctt
     2461 gagtgtttcg gcaacttcct cggtatttcc ccggagcgaa gttttgctct cgccttcgga
     2521 aaaatcgtaa ggatacatat tggccagatg ccggacagcg gattcgctct gcactttgac
     2581 aaaggaatca tagttttcca catcaaatga cgccctgaaa gtatcgttga ctttccagac
     2641 aatgacggcc gcaatttcga tgggattccc gatctgatca ttgaccttaa gcttctcgcc
     2701 gttcagattg cgggaacgga gagaaatttt cttttttgaa taaaaagggt tgacccataa
     2761 aattcccgct tggcggaccg agccgcggta atcgccgaac aggctcaaaa cggcggcctg
     2821 gtttggcgcg agattaaaga aaccgctcat gaacaaaaag aatagaatgg caaagagaac
     2881 accggctacg attaaaagcg gaaccgccgc gttcattttt gggttgtcgg cgaaaacacc
     2941 caggccgaca aaagacaagg ccgaaagaat caggaaaaga aaggtccaga atagataaat
     3001 ccagcccgtt gaaaccctga tgtttttttc ctttgcatga tctgttgaca tagaatcatc
     3061 tcctttgata ttgttatgat atcataataa tatctgaatg taaatatatt ccggtcgact
     3121 cccgaaaata ttattgttta ttccggccac tgaccagtct tgctgaattt ggcggtaagg
     3181 atcattcagg tagtttacaa aatcattaaa aacaggttat aatagaaaca ggacaagatt
     3241 tgacaaattg tagagaggta acgccgtgag cgaatttatt acaaatattg cttatcatat
     3301 taacctgttc ccgctgtggg ccgtttttct gtttgttttt ctatccgcct gtatccagca
     3361 gttttttccg ccgtatccgt cggaaatttt attgttgttt ttgggctgcc tcgctgcgtc
     3421 cagactgctg cccggcgttc ctgttattgc catttacgcg tttggaacgg tactttcaag
     3481 cctttttgtt tttcatcttt cgagacgcgg aggaaagcag cttctaaaga ataaatttgt
     3541 aatgaaattt tttccgagga aatatcagct aaaagcgggc gtttatgtca gacggtatgg
     3601 tattttggct ctgatcttct gtaagtttct gcccggtgtt aatacactta gtttaattat
     3661 gggcggaatt atggggctca agggtccggc ggcctggttg agtatcagca taaccggaat
     3721 tgtggcgaat gtggtttact ttttagccgg aacaattatc ggaaacaaca tacctgcgtt
     3781 gtatcatttt tcaaaaaatt tcagcattgg cgctatgatt gcggtggccg tcataatagc
     3841 agccgcggct gctttttatc tggtcggaaa atatatggcc gggaggaaaa agaagccggc
     3901 agcaagcgaa taaaagcaaa atcatgacat taaactttta ctgccataaa ttatttgatt
     3961 tattttcgtg aaaaagcaga aaataaacat gccgcgcgca aaacttgttc agggggtggc
     4021 ggcatgacaa aaatcagatt tttttcggca gtattgattg gctggctgtt gatctgcccg
     4081 gttcaggcag cgtcaagcct taaggtttat ggtttttcca cagagtttaa agaaccgtcg
     4141 ctgcaataca gctgctcgta ccctcgaatt accggaattg acaataatca gatgcagcag
     4201 cgtgtgaacg tgatgatccg tgaaaaagcg gaaacatcgg tgcaaaccgc ccaatatatt
     4261 tccaaaaagc tgaatggggc ggttaaagtc agcggaaagt ttcattatca gcttaaaaga
     4321 aatgaaaacg gtattttgag ccttgttttg caggatagcc ttttcaccgg aaatgaacgc
     4381 ggaattcagc agcagacagg cgtcacgctg aatacggtaa acggcagaat ttataaactc
     4441 agaagccttt ttgtggagca tgcggattat gtcggggtaa taagcgatga gattcatcgt
     4501 cagatttctc aaaaaggcct gaaaaatcaa cagctggaga gttttaaaaa aatcagaaac
     4561 gatgagaatt tttatttgac atcggattcg ctggttgttt tttttgatca gtatcaatat
     4621 ggcaacagtg atatccggga gttctcaata ccgctcaatt cccttaatgg aatgctcaag
     4681 ccggaattcc ggcttttgac ctgaacttcg taaattgaat agcgcgcgga aaaaaccctc
     4741 ctgcattctg cgggagggtt ctgtttttgc tgtaatttgg cgtatctttc atgtgtttca
     4801 gctgttaaat ccccggccca tcagggcggt taccagagca aaaagcccga ccagatccag
     4861 aatggtgcct ttcagatgtt ctttctttac aagaaaccac caggagccgt taaacaggca
     4921 gaaaacagcg aggatcaccc ggtctgcaat gcccagagaa tgatgccagt tctgaaccag
     4981 atagcctaaa atcagaacta cggcaaagct caggagcagc agaaaattaa aaagataatc
     5041 caaacgaatc gttttacccg cgtcgttcat tttttcctcc agccggattt gatttaaatt
     5101 gcaaaataat cgacgcccgc gctgaaaatg cgctgatctt tattgccgcc gatgtttttt
     5161 gccacatatt ttccgattct ttcactgtgc gccatttttc cgagaattct gccgtccggc
     5221 gacgtgatgg cttcgattgc ttcaaccgag gcgttggggt tgtaatggat atccatcgac
     5281 ggtctgccct gaagatcgac atactgggtc gcaatctggc cgttttgggc aagcttctcg
     5341 atcagctcgg aagacgccac aaatcttccc tcgccgtgag agaccggaat cgtgtaaacg
     5401 tcgccggggt ttgtatacat aaaccagggg gatttgaccg agaccacttt ggttcgaacc
     5461 atggtggact gatggcggcc gattgagtta aaggtcaggg tcggacagtc ccggtccatc
     5521 tcctttattt cgccatatgg cacgagacca agtttgatca gcgcctggaa gccgttgcag
     5581 atacccagca tcaggccatc gcggttttgc agcaggtcgg cgaccgcgtc cttgacccgc
     5641 ggattgcgga aaaaggccgt gataaacttg ccggagccgt ccggctcgtc gccgcccgag
     5701 aaaccgcccg ggatcatgat gatctgactg ttacggatat gtttttcgaa ttctttgacc
     5761 gaatcctcta cggcaagggc agtcagattg cggatgacga aggtgtcgca gaccgctccc
     5821 gcctgctcaa actggcgcgc cgtgtcgtat tcgcagttgg ttccggggaa taccgggatc
     5881 agaacgcgag gcgaggtgac gtgcgccttc ggccgggcgt ttcccctgag gttatagtta
     5941 aaggtgtggg cgctgtcgct ctgcgctttc gcgttggtcg ggaagatttc ttccagaggg
     6001 gcttcccatg atttttcgag atcgtcaagg ttcatggtga ttccgccgat ttcaatctgg
     6061 ggctgggaaa tcgtatggcc cagcagcgtt ccgcgaatca gggctttgcg gtcgacttcg
     6121 atgagaaaac cgccgtagca ttggtcaaac agcagattgc ggtcgaggtt tttcaccgac
     6181 tgaaatccga tccggttgcc gaacgccatt ttggtgatgc cttccgcggg gccggcggac
     6241 gataaggccc aggccgcctt gattttcctc tgggaaatga gtttttcgac tcggtcgaag
     6301 agcctgcgga cactgccgaa atcaggcatg ccgtttttga gatatttagg ccggagaaga
     6361 agaattttgt cgccctccgt tttaaactcg ggagatatga ccttttgcgc gttcgtgacg
     6421 gagcaggcaa aggaaatcag cgtgggaggc acgtcgattt cttcaaagct tccggacatg
     6481 gagtccttgc cgccgatcgc cgcaataccg aggccggtct gcgcttcata ggcgccgagc
     6541 agggcggcaa acggtttgcc ccagcgggac gggtcgtttt tcgtgctctc gaaaaattcc
     6601 tgaagggaca gccatgcttt tttccggctt ccgccggcgg cgacaatacg ggctaccgac
     6661 tcaaccacgg caaccgccgc gccatgatag gggctggcgg aagatagtac ggggttaaat
     6721 ccccacgcca tcagcgaaca ggttgtagtc gagcctttta acacagggag cttcgcagcc
     6781 attgcctgaa tcggcgtgga ttggtgctca ccgccgaagg gcatcaggac ggttgaagct
     6841 ccgatggagg aatcaaaacg gtcggaaagg cctttttgag aacaaacatt aaggtctgag
     6901 agcatttcct tccatctctg ttcgagatcg gtgaaaggct gggaaagata gagggaagcc
     6961 acatcgcagt cgtgaacttt tattccggtg tgtttctccg caccgttgga gtttagaaag
     7021 tcccttgaaa tatttacaat ttcggagccg ttccaataca ttctcagcct gggctccgcg
     7081 gttacaaccg cgactgctgt ggcttcaagg ttctccttgg ccgcaagctc aataaatttt
     7141 tcagtgtctt tttcccgaac gactaccgcc atgcgctcct gagattcgga gatggcgagc
     7201 tcggttccgt cgagcccgtc gtatttgcgg ggcacggcgt taagatctat tttcagcccg
     7261 tcggccagtt ccccgatggc gacgctgacg ccgcccgcgc cgaaatcatt gcagcgcttg
     7321 atcatggtgg tgactgtcgg gtggcggaaa aggcgctgaa tcttacgttc ttcgggcgcg
     7381 ttgccctttt ggacttcggc tccgcagctc tcaagcgatt cgacagtgtg ggatttggag
     7441 gagcccgtcg cgccgccgca tccgtcgcgt cccgttctgc cgccgagcag aacgacgatg
     7501 tcgcccggtt ccggaatttc ccgcacaacg ttttccgccg gggcggctcc gatgacggcg
     7561 ccggtctcca gccgtttggc gacgtagccc ggatggtaaa cctcgctgac ctgaccggtg
     7621 gccaggccga tctggttgcc gtaggagctg tagccgtggg cggctccgac cgaaattttg
     7681 cgctggggca gtttgccttt catggttttc tcgaccggca gccttgggtc ggccgcgccg
     7741 gtgacacgca tcgcctgata gacgtaggaa cggcccgaaa gcgggtcgcg gatcgcgccg
     7801 ccgagacagg tggcggctcc gccgaaaggc tcaatttcag tggggtggtt atgggtttcg
     7861 tttttgaaca tcagcagcca gtcttccata ttgccgtcga cgtcggcctt gattttgaca
     7921 gagcaggcgt tg
//
LOCUS       DATALY010000293         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_64370,
            whole genome shotgun sequence.
ACCESSION   DATALY010000293 DATALY010000000
VERSION     DATALY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64370"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..909)
                     /gene="dapF"
                     /locus_tag="VHR42_07980"
     CDS             complement(76..909)
                     /gene="dapF"
                     /locus_tag="VHR42_07980"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX3027148.1"
                     /translation="MKFTKMQGIGNDYIYINCFEETVKEPSALSVRLSDRHFGVGSDG
                     VILIEPSKIADCKMDMYNADGSRGMMCGNGIRCVGKYVYERGIARKDVLTVETLSGVK
                     TLRLDVQDGKVGEIEVDMGSPILKPEQIPVRFPGETAVDLPFSVGGTEYRVTCVSMGN
                     PHCIVFVPETKSFPVEKIGPQFENDPHFPERVNTEFVQILGSGEVNMRVWERGSGETF
                     ACGTGACATAVACALNKKTGRNVLIHLLGGDLRIRWDEVSGSVFMKGPAEFSFDGTVE
                     I"
     gene            complement(921..1463)
                     /locus_tag="VHR42_07985"
     CDS             complement(921..1463)
                     /locus_tag="VHR42_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX3027149.1"
                     /translation="MSSIVVANSNPDFAKKIASVLHSNGLYIGGTCVSGAQLIDFTKK
                     HYHGGVVISSVKLKDMPAVNLPRMIGPRYDFVFVVSPQFSGMSESIEYASLLMPINRM
                     SLVTTVNMFLNLAEETPASIKKKIENGTLDDKQIIQQAKNILISRNNLTEPQAHRLIQ
                     KKSMDTGRKMIETAMIILRS"
     gene            complement(1499..2830)
                     /gene="glnA"
                     /locus_tag="VHR42_07990"
     CDS             complement(1499..2830)
                     /gene="glnA"
                     /locus_tag="VHR42_07990"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX3027150.1"
                     /translation="MPKYTREDILRIVQEEDVKFIRLQFTDILGTLKNVTITPGQLKK
                     ALDNQCMFDGSSIEGFVRIEESDMYLHPDIDTFSIFPWFSQNGRIARLICDIYLPDGS
                     PFEGDPRYVLRKEMQKASKLGFTFNVGPECEFFLFNTDEFGHPTTVTHDTAGYFDLGP
                     SDLGESCRRDICLNLEEMGFEIEASHHEVAFAQHEIDFKYSDALSAADNLLTFKLVVK
                     TSAEANGLCATFMPKPITGRAGSGLHVNMSLFRGENNAFYDPKGDLKLSSDSLSFIAG
                     VMKHIRGICAVTNPLVNSYKRLVPGFEAPCCIAWTTGNRSALIRIPESRGTGTRVELR
                     NPDPAGNPYLSFALLLAAGLDGIEHHLQPIAPVSANVYGISEEEKQNCGIGSLPADLN
                     EALSEMKADPFVKETLGSHIFQKYIEAKEREWQEYSSTVTEWESERYLSKF"
     gene            3149..>3891
                     /locus_tag="VHR42_07995"
     CDS             3149..>3891
                     /locus_tag="VHR42_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX3027151.1"
                     /translation="MSYVDDQLNELIRTNPNQPEFIEAATVVLKSLEPVVEADEKYRK
                     SGILERLTEPDRQIRFRVAWTDDAGHVQVNRGYRVQFNNAIGPYKGGLRFHPSVNISI
                     IKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDREVMAFCQSFMNELYRHIGPDVD
                     VPAGDIGVGGREIGYLYGQYKKITNHYEGILTGKGLPYGGSLARTEATGYGLLYFLDA
                     MLKANGKSLKGKTVVISGAGNVAIYACEKA"
BASE COUNT          975 a          992 c          929 g          995 t
ORIGIN      
        1 ggttacggac ccgaagcggg ttatgcgttc ctacgtgagg caatctgcaa aaacgatttt
       61 caaagcaata ttatatcaga tttcgacggt tccgtcaaag gaaaattcag cagggccctt
      121 cataaagacg cttccggata cttcatccca gcgaattcta aggtcaccgc caagaaggtg
      181 aataagaacg ttccggcccg tttttttgtt tagggcacag gcaaccgcag tagcgcacgc
      241 tcccgttccg caggcaaaag tttcaccgga accacgttcc caaacgcgca tattgacttc
      301 gccgctgcca agaatctgca cgaactccgt attgacacgc tccggaaaat gcggatcgtt
      361 ttcaaactgc gggccaattt tctcaacagg aaacgatttc gtctccggca caaaaacgat
      421 acaatgcgga ttgcccatgg aaacacaggt tacccggtac tctgttccgc caacggaaaa
      481 cggcagatcg acggccgttt ctccgggaaa tcgcacggga atttgttccg gctttaggat
      541 tgggctgccc atatcgacct caatctcacc gactttgcca tcctgaacat cgagccggag
      601 ggttttcacg ccgctgagtg tttcgacagt caaaacatcc ttgcgggcaa tgccgcgctc
      661 ataaacatat ttacccacac agcggattcc gttcccgcac atcatgccgc gggaaccgtc
      721 tgcattatac atatccattt tacaatcggc aatcttcgag ggttcaatta ggatcacgcc
      781 gtcggaacca acgccaaaat ggcggtcact caggcgaacg gatagcgccg aaggttcttt
      841 gaccgtttct tcaaagcagt tgatgtaaat ataatcgttg ccgatgccct gcatcttagt
      901 gaatttcata aagtcgctcc ttatgatctc aggataatca tggctgtttc aatcatcttt
      961 ctgcctgtat ccatactctt tttctgaatc agccggtgcg cctgcggctc tgtcagattg
     1021 ttccggctga tcagaatatt tttcgcctgc tggataatct gcttgtcgtc aagcgtgccg
     1081 ttttcaatct tcttcttaat ggaagcaggt gtttcttccg ccagattgag aaacatgtta
     1141 acagtggtca ccagactcat ccgattaatc ggcataagca ggctcgcata ctcaatggat
     1201 tcgctcatcc cggaaaactg cggacttacc acaaagacaa aatcatatct tgggccaatc
     1261 atccggggaa ggttgaccgc aggcatgtct ttgagcttta cgctgctgat aacgacccca
     1321 ccatggtaat gctttttggt gaagtcaatc agctgtgcac cgctgacgca ggttccaccg
     1381 atatacagcc cgtttgaatg caaaacggac gctatttttt ttgcaaaatc aggattggaa
     1441 tttgctacga caatgctgct cattctgata accccatgtg aatggattta tggttctgtt
     1501 aaaacttgct cagatacctc tcggattccc attctgtcac cgtagacgaa tactcctgcc
     1561 actcacgctc ttttgcttcg atgtatttct ggaaaatatg gctgccgagc gtttctttta
     1621 caaaggggtc ggctttcatt tctgacagcg cctcgttcag gtcagccggt agactgccaa
     1681 tgccgcagtt ctgtttctct tcttcgctga tgccgtaaac attggcggaa acgggagcaa
     1741 tcggctgaag atggtgctcg atcccgtcaa ggcctgcggc aaggagaagc gcaaaagaaa
     1801 gatatgggtt acccgcagga tcggggttac ggagctccac ccgcgtaccg gtcccgcgag
     1861 actccggaat gcggataagc gcactgcgat tccccgttgt ccaggcaata cagcaggggg
     1921 cttcaaaccc gggcacaagg cgtttatagg aattgacaag cggattcgtt acggcgcaga
     1981 tgccccgaat gtgcttcata acgccggcaa taaaactgag gctgtcgctg ctcagtttca
     2041 gatccccttt cgggtcgtaa aaagcattgt tctccccgcg gaaaagcgac atgttgacat
     2101 gaagcccgga gcctgccctg cccgtgatcg gtttcggcat aaaggtggca caaaggccgt
     2161 tggcctcggc agatgttttg acaaccagtt taaatgtcag caggttatcg gccgcagaaa
     2221 gtgcgtcgct gtatttgaaa tcaatctcat gctgtgcaaa tgcaacttca tggtgggaag
     2281 cctcaatctc aaagcccatc tcctcaagat tcaggcaaat gtcacggcgg cagctctcgc
     2341 caagatcgga aggtccaagg tcaaaatacc ccgccgtatc gtgcgtcacc gtggtcgggt
     2401 ggccgaactc gtctgtgttg aaaagaaaga attcgcactc cggccccacg ttaaatgtga
     2461 acccgagctt tgaagctttc tgcatctctt tgcgcaggac atagcgcggg tcaccctcaa
     2521 acgggctgcc gtccggcaga taaatgtcac agatcaaacg tgcaattcgc ccgttctggg
     2581 aaaaccacgg gaagatagaa aacgtatcga tgtccgggtg gagatacata tcggattctt
     2641 cgatgcgcac aaatccttca atagatgagc catcgaacat gcactggttg tcgagggcct
     2701 ttttgagctg tcccggtgta atcgttacgt ttttcagcgt accgagaata tccgtaaact
     2761 ggagacggat gaatttcaca tcttcttcct ggacgatgcg aagaatatct tctcttgtat
     2821 acttaggcat ccggatccct cctaaaaaaa taataacaag gactttccgt tcaaagcctc
     2881 cttgttattt ttcgtaccct tgaattgttt ttattctatt ctatttccta ttgtgtgtca
     2941 aatgtttttg ccgcctttca gcgcagtaaa aagcatggtt ctttggttcc agaattctta
     3001 catttattaa caattatcat gcatgaatgt cctataataa agtgcactct gttgttatat
     3061 cagcctttat atggtattat gttaattatt gtaggtgatt gagccttttt gtcaccttaa
     3121 tacactttta tataggggga tacaagccat gtcttatgta gacgatcagc taaatgagct
     3181 gatccgtact aacccaaacc agcctgaatt tatcgaggct gcaaccgtag ttctgaagtc
     3241 gcttgaaccg gtcgtggagg cagatgagaa gtaccggaaa tccggaattc tcgagcgtct
     3301 taccgaaccg gaccgacaaa tccgcttccg tgtcgcatgg accgatgatg ccggccacgt
     3361 tcaggtaaac cggggatacc gggtacagtt caacaacgca atcggtccct acaaaggcgg
     3421 gctgcgtttt catccctctg tcaacatcag catcatcaag ttcctcggct ttgagcagat
     3481 tttcaaaaat tccctgaccg ggctgccgat cggcggcggc aagggcggct ccgatttcga
     3541 cccgaaaggt aagagcgacc gcgaagtcat ggctttctgc cagagtttta tgaacgagct
     3601 ttaccgccat atcggccccg acgtcgacgt tccagcaggc gacattggag taggcggcag
     3661 agaaatcggc tacctctatg ggcaatataa aaagattaca aaccactatg aaggaattct
     3721 caccggcaaa ggcctcccgt acggtggttc gcttgcgcgg acggaggcca ccggatatgg
     3781 cctgctgtat tttctggacg caatgctgaa agcaaatggg aaatcactga aaggcaaaac
     3841 cgttgtaatt tcaggcgccg gaaatgttgc tatttatgcc tgcgagaaag c
//
LOCUS       DATALY010000294         4177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_151775,
            whole genome shotgun sequence.
ACCESSION   DATALY010000294 DATALY010000000
VERSION     DATALY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151775"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..768)
                     /locus_tag="VHR42_08000"
     CDS             complement(184..768)
                     /locus_tag="VHR42_08000"
                     /inference="COORDINATES: protein motif:HMM:NF021062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEX3027152.1"
                     /translation="MAATSGLNNNVRRLVESAIMIALASILSIIPFYKMPLGGSMTLA
                     SMLPIMIIAYKYGVKWGLFTGFCYAIIQLLIDAGAVLSWGLTWQAVAGSFTLDYILAF
                     TSLGLAGIYGKGFARFMAGMTTAVAVRFICHVVSGVIIFSSYAADKSVPAVIRGHVFL
                     YSAAYNGAFLLPDLILCLVVGAVIFKPLGRFLND"
     regulatory      complement(836..949)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1095..1283
                     /locus_tag="VHR42_08005"
     CDS             1095..1283
                     /locus_tag="VHR42_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3027153.1"
                     /translation="MQTILYYFLNLLYTIPVLLIAFPVHECSHALVAKWMGDPTAKNM
                     GRLTLNPMKHLDLFGTIC"
     gene            1356..1727
                     /locus_tag="VHR42_08010"
     CDS             1356..1727
                     /locus_tag="VHR42_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3027154.1"
                     /translation="MAITALAGPVSNFIMGFLSVFFLLKLQVWVPGTPDIVLTFLYYS
                     AIINTGLSIFNLIPIMPLDGSRILAYFLPERLEAALAQYGTYIQLGLLLLLFTGILSG
                     PLSTMVNTLLKNFDYLVRLIP"
     gene            1804..2538
                     /locus_tag="VHR42_08015"
     CDS             1804..2538
                     /locus_tag="VHR42_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX3027155.1"
                     /translation="MEKLSFRLEVFEGPLDVLLYLIAKHKLNIYDIPIAELLSQYLTY
                     LGVMREMDLDVTSEFLEMASRLVQIKSSMLLPKYEGEDGEDPRSELMATLIEYQTCKQ
                     MAKSLAARAGGFDCFTREPITLEQDLTYRRRHLPVELYEAYLSAAGKAKRRLPPTISA
                     FRGIVGRKIVTVASRVVHIVRRLISEGKKDWDELFEDATGRSEAVATFLAVLELVKSK
                     KIIVEQEGRDQTVRAVRRVGRRLRVK"
     gene            complement(2733..3074)
                     /gene="ytfJ"
                     /locus_tag="VHR42_08020"
     CDS             complement(2733..3074)
                     /gene="ytfJ"
                     /locus_tag="VHR42_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HEX3027156.1"
                     /translation="MSEHPIQGLMNTALQNIRDMIDVNTIIGEEIVASDGTVIIPVSK
                     VSLGFASGGSDIPNKLEKDIFGGGSGAGVSINPVAFIVVSNGEVKLRLNLRGKFRTGG
                     QLKRIIQTTDL"
     gene            complement(3071..3727)
                     /locus_tag="VHR42_08025"
     CDS             complement(3071..3727)
                     /locus_tag="VHR42_08025"
                     /inference="COORDINATES: protein motif:HMM:NF022615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HEX3027157.1"
                     /translation="MPLIVTGAVVLGILILLMIPVGVYASYDGKTVLKVKILFFQFRM
                     IDEKTGKAKTGKKKKKKERKQREKPEKTEQKQPLKIPDLIRLIEQFSDPAKKLLRSFP
                     NLLTVRRLKFRLTVCEQDAALTAMLYGGACAAVYPALSFLISHVRVKKHQVEILPHFQ
                     PDAQNAGSKVEFETEVSARPLSLLAVTIRAAAGMAAAILKNRARRNRTNKMAKGGAVK
                     "
     gene            complement(3785..>4177)
                     /gene="scpB"
                     /locus_tag="VHR42_08030"
     CDS             complement(3785..>4177)
                     /gene="scpB"
                     /locus_tag="VHR42_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX3027158.1"
                     /translation="GGLKIVRLDDSFQMTARPEFAPQIKQVLDIRRNTPLSQAAMEVL
                     SIVAYHQPVTRGFVEQVRGVDSSGIMSNLVGKGLIEETGRLDVPGRPLLYGTTLDFLR
                     CFGLGSLSDLPPVQNGGESEEDGNENAG"
BASE COUNT          992 a         1092 c         1059 g         1034 t
ORIGIN      
        1 tcctttattc cgcggcctat aacggcgcat tcctgctgcc cgacctgatt ctctgtctgg
       61 tggtcggcgc tgtgatcttc aaacccctcg gccgtttcct taacgactga gtcacccggt
      121 gacgaatcgc agagcggcat ccctgagtaa gggatgccgc tctgcgattc gccaccgggt
      181 gactcagtcg ttaaggaaac ggccgagggg tttgaagatc acagcgccga ccaccaggca
      241 gagaatcagg tcgggcagca ggaatgcgcc gttataggcc gcggaataaa ggaatacatg
      301 gccccggatg actgcgggaa cggatttgtc cgctgcgtag gaagagaata tgatcactcc
      361 ggaaacgacg tggcagataa accggacagc cacggcggtc gtcatgccgg ccataaatct
      421 ggcaaagccc ttgccgtaga ttccggcaag accaaggctt gtgaaggcga ggatgtagtc
      481 aagcgtgaag gagccggcta cggcctgcca ggtcagaccc caggagagaa cggcgcccgc
      541 gtcgatgagc agctggatga ttgcatagca gaatccggtg aaaagacccc actttacgcc
      601 gtatttataa gcgataatca taattgggag catgctcgcc agcgtcattg atccgcccag
      661 cggcatttta taaaacggga tgatactcag aatgctggcc agggcgatca tgattgcgct
      721 ttccaccaaa cggcggacgt tgttgttaag cccggaagtt gctgccataa aaaaattccc
      781 ccaaaataaa attaaaagac cacaccggtt tcccggcgcg gtctcaacat ctgaacaggt
      841 gaatcagatc aattcctacg ccggcattat ccggatcagg ttccagggtt aggaatatca
      901 attggattcc actctcagcc gaactcattc agcaccccta tatttttatg ctcattatac
      961 agcccgcctg cctgcgtgtc aagacctggc ccgccggtat tgttttttgt tacgccatga
     1021 attataataa aaagaagata ttgtctgttt ttttactggc tgatactgat tattaactgc
     1081 gcatggaggt ttttatgcag acgatcctat attattttct caatttgctc tataccatac
     1141 cggtcttgct gattgccttt ccggtgcacg agtgttccca tgcgctagtc gcgaaatgga
     1201 tgggggaccc gaccgcgaag aatatgggcc ggctgacgct taacccgatg aaacatctcg
     1261 acctgtttgg aaccatctgc tagattttta ccaatttcgg ctgggcaaag cctgtccccg
     1321 ttaacccgaa taattttcga aaccgcaagg aaggcatggc gatcaccgct ctcgcgggcc
     1381 cggtttccaa ctttatcatg ggctttttgt ccgtcttttt ccttttaaaa ctacaggtat
     1441 gggttccggg aacccccgac attgtgctga ctttccttta ttattccgcg atcatcaaca
     1501 cgggtctttc tatttttaat ctcatcccaa ttatgccgct ggacggctcc agaatccttg
     1561 cctattttct tcccgaaagg cttgaggccg cacttgccca atacggaacc tatatccagc
     1621 tcggtctttt gctgctcctg tttaccggta ttctttccgg cccgctgagc acaatggtca
     1681 acacgcttct taaaaatttt gactaccttg tccgtctgat tccctaaacc ggccgggaaa
     1741 gcaggatcga accggagcgg ggcgcttctg tcagcgccca aaggaagtat aatggagaaa
     1801 aatatggaaa agctttcatt taggctcgaa gtttttgaag ggcctctgga tgttttgctt
     1861 tatctgatag cgaagcacaa actgaatatt tatgatattc ccattgccga gctgcttagt
     1921 cagtatctga cctatctcgg cgttatgcgg gagatggatc ttgacgtcac cagcgagttt
     1981 ctcgaaatgg cgtcccgtct ggttcagatc aagtcgtcga tgctgctccc gaaatatgag
     2041 ggggaagacg gggaagaccc ccgcagcgag ctgatggcta cgctgatcga atatcagacc
     2101 tgcaaacaga tggcgaagtc tcttgcggcc cgcgccggcg gattcgactg ctttacgcgt
     2161 gagcccataa cccttgagca ggatcttaca taccgccgcc ggcacctccc cgttgaattg
     2221 tacgaggcgt atctgtcagc ggcaggaaaa gcaaaacgca ggcttccgcc taccatttcg
     2281 gcgttccggg ggatagtcgg aagaaaaatt gtgacggtag cttcacgggt tgtccatatt
     2341 gtcagaaggc tgatctcgga agggaaaaaa gattgggatg agctttttga agacgccacg
     2401 ggccggtcgg aagctgtcgc aacatttctg gctgtgctgg aactggtgaa atcaaaaaaa
     2461 attattgtcg agcaggaggg gcgggatcag accgttcgtg ccgttcggcg cgtcgggcgc
     2521 aggctgcgcg taaaataatc cggagtaaac gacaaggatg aacagatgaa atggaaatta
     2581 ataaaataaa atcgtcgatt gaagccatcc tgttcgcgag cggggaaccg gtatccattg
     2641 atcgtctgtc gcagacgctt gaaatcgacc ggaataccgt attaaatatc gcgacccttt
     2701 tcgcggaaga attaaacggc cgtgagggcg ggctaaagat cggtcgtctg gatgattcgt
     2761 ttcagctgac cgcccgtccg gaatttgccc cgcagattaa gccgcagctt tacttctccg
     2821 ttcgatacaa cgatgaacgc aaccgggtta atgctgacac ccgctccgct tcctccgccg
     2881 aagatatctt tttccaactt gtttgggatg tcggagcctc ccgacgcaaa accgagggac
     2941 actttggaaa cggggatgat aactgtcccg tcggaagcga caatttcctc tccgataatc
     3001 gtgttgacgt caatcatgtc acggatattc tgcaatgctg tgttcatcag gccctggatc
     3061 ggatgttcac tcattttact gcaccgcctt ttgccatttt atttgttcta tttcttcttg
     3121 ctctgttttt taaaattgct gcggccatac cagcggccgc gcggatggta acagccagaa
     3181 gactcagagg tcttgccgaa acttccgttt caaattcaac ctttgagcct gcattttggg
     3241 cgtcgggctg gaaatgcgga aggatctcga cctgatgttt ttttactctg acatgggaga
     3301 tcaggaagga cagagccgga taaaccgccg cgcacgctcc gccgtataac attgccgtaa
     3361 gcgcggcatc ctgttcacag acagtcagcc gaaactttaa ccggcgaacg gtcagcagat
     3421 tgggaaacga gcggagaagc tttttggccg ggtcggaaaa ctgctcaatc agccggatca
     3481 aatccggaat tttaagcggc tgtttctgct cggttttctc cggcttttca cgctgcttgc
     3541 gttctttttt tttctttttc ttcccggtct tagcttttcc tgttttttcg tcgatcatac
     3601 gaaactgaaa aaacagaatt ttgactttta acaccgtttt gccgtcgtag gaagcataaa
     3661 cgccgaccgg gatcattaaa agaatcagga ttcccagcac aacggcgccg gtcacaatta
     3721 aaggcatatc tcacctctcg ctacgtccgt ttctttcccg tcaatggaat ttgtaacggc
     3781 cgggtcagcc ggcgttttca ttcccgtctt cttcggattc cccgccgttc tgaaccggag
     3841 gcagatcgct gagggaaccc aggccgaaac accggaggaa atccagcgtg gttccataaa
     3901 gaagcggcct gcccggaaca tccagcctgc ccgtttcctc aatcaggccc ttgcccacca
     3961 gattggacat gatccccgag gaatccacgc cccgaacctg ctcaacaaag cctctcgtaa
     4021 ccggctgatg ataagcgaca atggaaagaa cctccatagc cgcctgtgac agaggtgtgt
     4081 tgcgtcggat atcgagcacc tgcttaatct gcggggcaaa ttccggacgg gcggtcatct
     4141 gaaacgaatc atccagacga acgatcttta gcccgcc
//
LOCUS       DATALY010000295         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_409016,
            whole genome shotgun sequence.
ACCESSION   DATALY010000295 DATALY010000000
VERSION     DATALY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409016"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VHR42_08035"
     CDS             complement(<1..807)
                     /locus_tag="VHR42_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3027159.1"
                     /translation="MLQINNLTITHLKDLRTILKNFSFTLNNGDKAVIIGEEGNGKST
                     LLKLIYNKNLISDYAEFTGEIVKNGIRFGYLAQELTPEQKSKSAAVFFQAIPSFTILT
                     HNELSGIALRLGLDSRIFYSDQTVGTLSGGEKVKLQLAALLMESPDVFLFDEPSNDLD
                     IETLEWLEHFIKTAKQPVIFVSHDETLIENTANVVIHIEQVRRKAMPRWTVARIPYRQ
                     YIEERLSRLSHQEQIAKEEQREYEKQQKNFRRIQEKVDHQLNTISRSDPHG"
     gene            complement(1059..2072)
                     /locus_tag="VHR42_08040"
     CDS             complement(1059..2072)
                     /locus_tag="VHR42_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3027160.1"
                     /translation="MPDKVDTRYLFRMTDDTGMFQHAVLGVPDPSEGYTSDDNSRALI
                     LAEMLYLKTKEQQYEDLMVRYMSYLIYAEKDRWFRNFMGYNRNFLEKRGSEDCFGRCI
                     LALGLTVSKDGLPAAVRECADRLLNRVASSCSSLTYLKGKAYALTGLTMWNSPDKQPL
                     IKMLIDSVIDTYVQCRREDWRWFEDTITYCSAVLPLSMFCASDYDRRCFGIGLESLDF
                     LLEITLRGGVFKPVGCKGWMKHGDIPAEFDEQPVEACGMMLACLKAFELTNDEKYYRN
                     ARQCFLWYVGENSLHLSLIDPETGGCRDGLTQYGLNGNEGAESIVCWLISALTAEKEH
                     WLY"
     gene            complement(2088..3233)
                     /locus_tag="VHR42_08045"
     CDS             complement(2088..3233)
                     /locus_tag="VHR42_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3027161.1"
                     /translation="MNGQQKIHVLFLSTYPPRACGIATFTQDLVGELKKTGKIDCEIV
                     AVNNGEYAYPSEVKFTLEQQNPNSYLEIAERINHSGADLLMIEHEYGIYGGKYGEYLL
                     NLTELLEIPYVVTLHTVLPDPTKKQKEIIRQLSEGCKNLFTMSSSSIPLLKEIYGVDP
                     NKIVMVHHGVPEIPVDNRDTLKKEAGFGGRYVVSTFGLLSPGKGLEYGIEAIARTAEK
                     HPEVLYLILGQTHPVIKSKDGEKYREKLEHTVESLGISKNVQFVNRYLTKEEIIRYLT
                     LSDIYMTPYLGKDQAVSGTLAYAVGYGRVIVSTPYPYAQEMLADGRGLLAKFKSASSL
                     TKCILEVLSHPDQQAEMERKTSELGKTMMWSRVAERYLSVFSPSASK"
     gene            complement(3269..>3652)
                     /locus_tag="VHR42_08050"
     CDS             complement(3269..>3652)
                     /locus_tag="VHR42_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX3027162.1"
                     /translation="PGKYMQAHRDIFNGLCAVSENNFLTDMVYGRADADIHPSAQIKG
                     PVWFGKNVHIGANATIGPNVVIGDGFQAGRDCTIRNSVIWNGVSAGSGIDITHSVITS
                     GCQIESGVQCSDTIYSQESKLPFTT"
BASE COUNT          877 a          894 c          869 g         1012 t
ORIGIN      
        1 cccatgcggg tcggaccgtg agattgtgtt aagctgatga tcaacttttt cctgaatccg
       61 gcgaaaattt ttctgctgtt tttcatattc tcgctgctct tcttttgcaa tttgctcctg
      121 atgggaaagg cgcgacaggc gttcctcgat atactgccgg taaggtatcc gagcgaccgt
      181 ccagcggggc atggctttgc gcctgacttg ttcgatatga atcacgacgt ttgccgtgtt
      241 ttcaatcagt gtttcgtcgt gcgaaacgaa aataacgggc tgcttggcgg ttttaatgaa
      301 gtgttccagc cattcgagtg tttcaatatc caaatcattg gagggttcat caaacagaaa
      361 cacatcgggg ctttccatta ataatgcagc aagctgcagc ttgacttttt cgcctccgga
      421 cagcgtgccg actgtttggt cggaataaaa aattcgggag tccaaaccaa gccggagtgc
      481 tatgcctgaa agttcgttat gggtcaaaat tgtaaatgag gggattgctt ggaaaaatac
      541 cgccgcactt ttgctttttt gttcaggagt taattcttgt gccaggtaac cgaaacggat
      601 gccgtttttg acgatttcgc cggtgaattc agcgtaatcc gaaatcaggt ttttattata
      661 aatcagtttg agcagggtgg acttgccgtt tccttcttcc ccgatgataa cggccttatc
      721 gccgttattc agcgtgaaag aaaaattctt cagaatcgtc cgaagatctt tcaaatgggt
      781 gattgtgaga ttgttgattt gcagcaatga aaccgtctcc ctaaaaaaat ctgctttcag
      841 ttaaaaccga aagcagattc atacacaggg aaaaaagcca taccgagtat ccggcaggac
      901 agcagctgct gcgtaattct ctaaatttcg gcaacagaac aagacctttt acgggtcttc
      961 gaaagcattc tgttttacac gaaaaataga ttatttacgc atttttccac ccatttcatt
     1021 cattttgctt tagattgtaa tacaacgcct gttttttgtc aatagagcca atgttccttt
     1081 tcggcggtca gagcggagat gagccagcag acaatgcttt cggcgccttc attgccgttc
     1141 aggccgtatt gtgtcaggcc gtcccggcag ccgccggttt ccgggtcaat caaggaaaga
     1201 tgcagcgagt tttctcccac ataccataaa aagcattgcc tggcgtttcg gtagtatttt
     1261 tcatcatttg taagctcaaa cgctttcaga cacgcaagca tcattccgca ggcttccaca
     1321 ggctgttcat cgaattccgc cggaatgtcc ccgtgtttca tccagccctt gcagccgacc
     1381 ggcttaaaaa ctccgccgcg cagggtgatt tccagcagaa aatcaaggct ttccagccca
     1441 atgccgaagc agcgcctgtc atagtcactt gcgcagaaca tggataaagg aagaacggcg
     1501 ctgcagtacg tgattgtgtc ttcgaaccaa cgccagtctt ctctgcggca ctggacgtat
     1561 gtatcgatga cagagtcaat cagcattttg atgagcggct gtttgtcggg gctgttccac
     1621 atcgtcaaac cggttagggc gtaagccttt cccttcaaat atgttaagct ggagcagctc
     1681 gatgcgaccc gatttagaag cctgtcagcg cattcgcgca cggcagcagg cagaccatct
     1741 ttggaaacgg taagcccgag tgcgagaata cagcgcccaa agcaatcctc ggaaccgcgt
     1801 ttttccagaa aattgcggtt atatcccata aaattgcgga accaccggtc tttctccgcg
     1861 taaatcagat aactcatgta acggaccatt aaatcttcgt attgctgttc ttttgttttc
     1921 aggtagagca tttcggcgag tatcagtgcg cgggagttgt cgtctgaggt atacccctcc
     1981 gacggatccg gcacacccag cacagcgtgc tggaacatgc cggtatcatc ggtcatgcgg
     2041 aagagatatc gggtgtcaac cttatcgggc atgcgttttc tcccttccta ttttgaagcg
     2101 gacggactga aaacggaaag ataacgctcg gcgacccggc tccacatcat ggttttgccg
     2161 agctcggaag tcttccgttc catttctgcc tgctgatccg gatggctcag tacttccaga
     2221 atacatttgg taagcgatga tgcgcttttg aattttgcaa gcagccctct tccgtccgca
     2281 agcatttcct gcgcataggg gtaaggcgtt gaaacaatca cacggccgta tccaacggca
     2341 taggcgagcg ttccgctgac cgcctgatct ttcccaaggt agggcgtcat atagatatcg
     2401 gaaagcgtca ggtaacggat gatttcctct ttcgtcagat accggtttac aaactgcaca
     2461 ttctttgaaa ttccaaggct ttcaacggta tgctccagct tttcgcggta tttttcgcca
     2521 tccttgcttt taatgacagg atgagtctgg cctaaaatca aatataggac ttccggatgc
     2581 ttttccgctg tgcgggcaat cgcttcaatt ccgtattcca ggccctttcc ggggcttaga
     2641 aggccgaatg tgctcacaac atagcggccg ccgaatccgg cttccttttt taaagtatca
     2701 cggttgtcta ccggaatttc cggaaccccg tgatgaacca taacgatttt atttggatca
     2761 accccataga tctcttttag cagcgggatg gaggaagaag acattgtaaa cagatttttg
     2821 catccctccg aaagctgccg tataatttcc ttctgctttt ttgtcgggtc aggcaggacc
     2881 gtatgaaggg ttacgacata tggaatctca agcagctcgg tcaggttcag cagatattcg
     2941 ccgtacttgc cgccatagat tccgtattcg tgttcaatca tcaaaaggtc ggctccggaa
     3001 tggttgatcc gctctgcaat ttcaaggtaa gaatttggat tttgctgctc cagagtgaat
     3061 ttcacttctg acggataagc atattcaccg ttattaaccg caacgatttc gcagtcaatt
     3121 tttccggtct tttttaattc tccaaccaaa tcctgcgtaa aggttgcaat tccgcaggcc
     3181 ctcggtggat aagtgcttaa aaaaaggaca tgaattttct gttgcccgtt caaaagtcac
     3241 agacctttct tttgtaaatt ttaaccggtt aagtggtaaa ggggagtttg ctttcctgcg
     3301 aataaatcgt gtcgctgcac tgtacgccgc tttcaatctg acagccggat gtgattacgg
     3361 aatgcgtaat atctatgccg cttccggcag aaacgccgtt ccaaatgaca ctgttgcgga
     3421 tcgtgcagtc cctgcctgcc tgaaatccgt cgccgattac gacattgggc ccgatggttg
     3481 cgttagcgcc gatgtgaaca tttttgccga accagaccgg ccctttgatc tgagccgacg
     3541 ggtggatatc cgcatccgcg cgcccgtaaa ccatatcggt cagaaaattg ttctctgaga
     3601 cggcgcataa gccgttaaaa atatcccggt gggcctgcat atatttcccg gg
//
LOCUS       DATALY010000296         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_65213,
            whole genome shotgun sequence.
ACCESSION   DATALY010000296 DATALY010000000
VERSION     DATALY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65213"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VHR42_08055"
     CDS             complement(<1..519)
                     /locus_tag="VHR42_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX3027163.1"
                     /translation="MCVAAFGQITQLMGKNAVVSFRGALKQVSAVLLPQAKIGDTVVV
                     HAGFATEIVKDTKKIYRDVVASDAYARQLLDAIEKENERFHGRELKIMNFCGTHENTI
                     VQYSLRELLPPNIKLLSGPGCPVCVTPEEEIAMGLQLAKRSNIIFTTFGDLLRVPTRW
                     GSLEQLRLHGIDV"
     gene            complement(554..898)
                     /gene="hypA"
                     /locus_tag="VHR42_08060"
     CDS             complement(554..898)
                     /gene="hypA"
                     /locus_tag="VHR42_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX3027164.1"
                     /translation="MHELAMVEGIYTVINEKMKEYDVRKIKQVKLMVGELTGVEDETM
                     KACFEMYVEETPLEGADLVLIRVPTKVRCRTCGNEYETKIPFAKCAVCGNKNIQIISG
                     KELYIDSLEVED"
     gene            complement(917..>1817)
                     /locus_tag="VHR42_08065"
     CDS             complement(917..>1817)
                     /locus_tag="VHR42_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon monoxide-induced hydrogenase"
                     /protein_id="HEX3027165.1"
                     /translation="RVCSLCSNNHPFTYCMAVEKIAGIEISERAEYLRTIADEVKRIA
                     SHLFNIGLIPHIIGFDSLFMHAMELRETMQDLKECIFGNRMNLSVNSIGGVRYDISDD
                     QAKYILKTLDNLKKPLEEMVKIYQSNASIRRRTEGVGVLPYDKAIEYGVIGPVARASG
                     LPYDVRVDNPYAAYDKVKVNPVTLKNGDVFSRVMVRVGEVVDSISIIEQCIKQMPEGP
                     TCLPYIPDIPIGEAIAKSEAPRGELIYYARSNGTPIPERLKWRVPTYQNWEALNTMLP
                     NYKIADIPVIVGSIDPCISCTER"
BASE COUNT          456 a          442 c          381 g          538 t
ORIGIN      
        1 gacatcaatg ccatggagcc ttaattgttc cagagaaccc catcttgtag gcacccttaa
       61 aaggtcccca aaggtagtaa aaattatatt gctccttttt gccaattgta accccatggc
      121 aatttcttct tcaggggtta cgcacaccgg gcagcctggg ccgcttaaca attttatgtt
      181 tgggggcaaa agctcccgca gggaatactg tacaatcgta ttttcatggg tgccgcaaaa
      241 gttcatgatc tttagttcgc gcccatgaaa ccgttcgttt tctttttcaa tcgcatcaag
      301 cagttgtctg gcatatgcat cggaagcgac aacgtcacgg taaatctttt tggtatcttt
      361 tacgatttcg gtcgcaaaac cagcatggac gacaaccgta tcaccgattt ttgcctgagg
      421 gagcagcaca gccgaaactt gctttagcgc tccacgaaaa ctgactactg catttttccc
      481 catgagttgg gtaatctgcc cgaaagctgc aacacacatt gttatcacct tttctgaaag
      541 cagaggaaag gattcaatcc tctacttcca gactgtcaat atataattct tttcctgaga
      601 ttatttgaat gtttttgttt ccgcataccg cacattttgc aaatggaatt tttgtttcgt
      661 attcatttcc gcatgtacgg caccgcacct tagttggaac tcgaatcaga acaaggtctg
      721 ctccttctaa cggtgtttct tccacataca tttcaaagca ggctttcatg gtctcatctt
      781 ccactccggt taattcgccg accatcagct taacctgctt tattttgcga acgtcatact
      841 ctttcatttt ctcattgatg acagtataaa taccttctac catggccagc tcatgcaact
      901 tgcggtacct ctttccttat ctttctgtgc aggaaataca cgggtcaatg ctgccgacga
      961 tgaccggaat atctgcaatt ttgtagttgg gcagcatggt atttaacgct tcccagttct
     1021 gataggtcgg tactctccat ttgagacgtt ctggaatcgg cgtgccgttt gaacgtgcgt
     1081 aatagatgag ttcgcctctg ggagcttctg attttgcgat tgcctcgccg atcggaatat
     1141 ccggaatata tggcaggcag gtggggccct ccggcatctg cttgatgcac tgctcaataa
     1201 tgctgatgga atccacgact tcgcctacac gaaccattac tctggaaaat acatcgccgt
     1261 ttttcagggt taccggattc actttgactt tgtcataagc cgcatagggg ttgtccaccc
     1321 tcacgtcata cggaagacca gaagctctcg ccaccggccc aataacgccg tattcaatgg
     1381 ctttgtcata gggaagaacg ccgactcctt cggtacgcct acggatggat gcgttcgact
     1441 gatagatttt caccatttct tcaagaggct ttttcaaatt atctaaagtt ttaaggatat
     1501 actttgcctg atcgtccgaa atatcgtaac ggacaccgcc gatgctgttg acggacaaat
     1561 tcattcggtt accgaaaatg cattctttca aatcctgcat agtttcacga agttccattg
     1621 catgcataaa aagagaatca aaaccgataa tatgcggaat taagccgatg ttgaaaagat
     1681 gggacgcaat gcgtttgact tcgtcggcaa tcgttcttag atattccgct ctttcggata
     1741 tttcaattcc ggcaatcttt tcaactgcca tacaataggt aaacgggtgg ttgttggagc
     1801 acagcgaaca aacgcgc
//
LOCUS       DATALY010000297         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_409953,
            whole genome shotgun sequence.
ACCESSION   DATALY010000297 DATALY010000000
VERSION     DATALY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409953"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHR42_08070"
     CDS             <1..252
                     /locus_tag="VHR42_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027166.1"
                     /translation="VQAVFRPMPEKSAFAPLDIVKWETRGSLASFVVRAGREETLAKL
                     NSMGAVFAEALPLTLEEVFISEMEVAGYDMENILAGTIQ"
     gene            215..2056
                     /locus_tag="VHR42_08075"
     CDS             215..2056
                     /locus_tag="VHR42_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027167.1"
                     /translation="MTWKISSPGRFSEFRNTYLWVLKKNIGMTVLLAVLMFLCMPLPT
                     IVAISGMKKIDSLTEAQWLADRAMHCRTMIMMEIEWAGVTLLLLFSIVFCVRLFGYMQ
                     NRRSVDLFHSFPVGRISMLLGRWCAGMTALVLPTLVNFLILRIVLLSFGISPIAGSMG
                     LVSAMFWVLLMAAAAFTFCMFLTVCSGSMMDTILSLLGINAGFPIGFLLCVQLVQLTL
                     PGMGIYWRDGDNVVPATLLAPFPAAYQPLLVGSALPAWFLPWWIFATAALLVGTCILY
                     RQRRSETAEDPTAFPIPKTIVRFLLTACAGIGIGLGLALVNAGMASFLIGVLAGSIVA
                     HVIVEALYARGFSTLKKSTAWYAVFAVCFLAFYGVLATGCFGYDTRVPDASEVESVSV
                     YIDSRQRQYISSNSGAIRLEPLLKQPESIRTILQIHKDLSGWYRSSHYPYAPGQQSGG
                     GLTLNYRLKNGGTLTRSYIPGSRDWKLIEKQSNAVAKLPEYEGESDLAFYLQPADMKS
                     FTISTDNHQGDTVTISGTSEKAELLKALREYCKNTTFDETENSLKNSNSLQVDWVTNI
                     SPDAHLREALGGYSGKILIESSEYLYHDGDVLDQAIQKIRTDFKSSK"
     gene            complement(2278..2532)
                     /locus_tag="VHR42_08080"
     CDS             complement(2278..2532)
                     /locus_tag="VHR42_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027168.1"
                     /translation="MVRYLGSADVPGVSIKYFVFGNRRHGYSVRISEENGECEYRFVS
                     PKLSEALDLANRLRRCTVFPENLSEILEDLRVAKVECSQR"
     gene            complement(2711..2848)
                     /locus_tag="VHR42_08085"
     CDS             complement(2711..2848)
                     /locus_tag="VHR42_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3027169.1"
                     /translation="MAAVILAFGIAFSRIFLFVHYPSDTLIGSLLGISCAFLIIFLYH
                     V"
BASE COUNT          686 a          666 c          750 g          746 t
ORIGIN      
        1 gtgcaggcag ttttccgtcc catgccggaa aaatcggctt tcgcgccgct cgacatcgtc
       61 aagtgggaga cgcgcggttc gctcgccagt ttcgtggtgc gtgcaggccg ggaagagacg
      121 ctggctaagc tgaattccat gggtgcggta tttgccgaag ctttgccgct gacccttgag
      181 gaagtcttta tcagtgaaat ggaggtggcc ggttatgaca tggaaaatat cctcgccggg
      241 acgattcagt gagttccgca atacttattt gtgggttctg aaaaagaata tcggcatgac
      301 ggttctgctc gcggttctga tgtttctctg catgccgctg cccacaatcg tggcaatttc
      361 cggtatgaaa aagatcgatt cgttaacgga agcgcaatgg ctggctgatc gagcgatgca
      421 ctgtagaaca atgattatga tggaaatcga gtgggccggt gtaacgctgc tgctgctgtt
      481 ttccatcgtg ttctgtgtgc ggcttttcgg ctatatgcag aataggcgca gcgttgacct
      541 gtttcattcg ttcccggttg gccggatttc gatgctgctg gggcgctggt gcgccggtat
      601 gacggcactc gttctcccta cactcgtcaa ttttttgatt ctgcgcatcg ttctgctttc
      661 gtttggaatt tcaccaatag ccgggagtat gggccttgta agcgctatgt tctgggtgct
      721 gctcatggcc gccgctgctt tcactttttg tatgtttctc acggtgtgca gtggaagcat
      781 gatggataca attctttccc tgcttggaat caacgcaggt ttccccattg gctttttgct
      841 ttgcgttcag cttgttcagc ttacgcttcc cggcatggga atttactggc gggatggtga
      901 taatgtggtg cccgcaacac tgctggcacc gtttccagcg gcatatcagc cgcttttggt
      961 gggttcagcc ctgcccgcgt ggtttctccc gtggtggatt tttgcgactg ccgcacttct
     1021 tgtgggtacg tgtattctct atcggcagcg caggagcgaa actgccgagg accccaccgc
     1081 gttcccaatt ccgaagacca tcgtacgttt tttgctgacg gcgtgcgcgg gtatcggcat
     1141 cggtcttggc ctggctcttg ttaacgcagg gatggccagt ttcctcatcg gtgtgctggc
     1201 gggttccatc gttgcacatg tcatcgtgga agcactttat gcacgcggtt tctctacatt
     1261 aaaaaagagt acagcatggt acgcagtgtt cgcggtgtgc ttcctcgcgt tctatggcgt
     1321 gcttgcgaca ggatgtttcg gctacgacac ccgtgtcccg gatgcatccg aggtcgaaag
     1381 tgtttctgtc tacatagaca gccgacagcg gcagtatatc agttcgaaca gcggggcaat
     1441 acggctggag ccgttattaa agcagcctga aagtatccgc accatactgc agattcacaa
     1501 ggatctttcg ggttggtacc ggagcagcca ttacccctat gcaccggggc agcagtcggg
     1561 cggaggattg accctaaatt accggttgaa aaacggcgga accctgaccc gcagctatat
     1621 tccaggttcc cgcgattgga agttgattga aaagcagagt aacgcagttg ccaaacttcc
     1681 agaatacgaa ggggaaagcg accttgcttt ttatttgcag ccagccgata tgaagtcgtt
     1741 taccatcagt acagacaatc atcaagggga tactgttacg atttcaggca caagcgaaaa
     1801 ggcggaattg ttgaaagcgc tgagggagta ctgcaaaaat acgacatttg atgagacgga
     1861 aaacagtttg aaaaattcaa attccttgca agtagactgg gtaacaaata tttctccgga
     1921 tgcgcacctc agagaagcac tggggggtta ttccggaaaa atattgatcg agagctcgga
     1981 gtatttgtac catgacgggg acgtgctcga ccaggcaatc cagaaaatcc gcacggattt
     2041 taagagttca aaataaagtt tatatccaaa acattgagcc ctattcaaaa gttatgactg
     2101 atatcggagg acggtatcat aaatagttaa cccctagcac agccgtggac gggtagagcc
     2161 ctgatccaca ggctgtgctt cttttgcagg acataataaa aggcgtccgg atgatttccg
     2221 gacgcctttt ctgtttcaga aagttgaggg aaaattgccg attttcacga caaccgctca
     2281 gcgttggctg cattctactt tagcaacccg cagatcttcc agaatttccg ataaattttc
     2341 cggaaacacg gtgcaccggc gaagcctgtt tgcaagatcc agtgcctccg acagctttgg
     2401 tgaaacaaag cggtattcgc attcaccgtt ctcctccgaa atacggacgc tgtatccgtg
     2461 tctccgattg ccgaatacaa agtatttaat gctgactccc ggcacgtctg cgctgcccaa
     2521 atatctgacc aatgttccgc cctccttttg ctgtgaaccc aattcacgtc tgccgtagca
     2581 acgacattat tttatgttaa atgcaacata aaagcaagag aaaaacggac tattttgtcg
     2641 ttaaattgtg atattcgcaa gatgtttgtt tctccagcgt aaatgacgtg tatcttgtgt
     2701 tgagagaact ttacacatgg taaaggaaaa tgattaaaaa tgcgcatgag ataccgagta
     2761 aggaaccaat aagcgtatca gaaggatagt gaacaaaaag aaagatgcgg gaaaaagcaa
     2821 tgccaaaagc gagaataacc gcagcaat
//
LOCUS       DATALY010000298         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_65932,
            whole genome shotgun sequence.
ACCESSION   DATALY010000298 DATALY010000000
VERSION     DATALY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65932"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2798
                     /locus_tag="VHR42_08090"
     CDS             <1..2798
                     /locus_tag="VHR42_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX3027170.1"
                     /translation="SIRENYAGRPVHVSVTGSAGLGVAKVCGLEFVQEVFATAGAVEA
                     YLPGTDAVIELGGEDAKIIFFTGGLEERMNGSCAGGTGAFIDQMATLLGVSTDELDAL
                     AAKHEKIYSIASRCGVFAKSDIQPLLNQGARKEDIAASVFQAVVEQTIAGLAQGRKIE
                     GKVAFLGGPLSFFSVLRERFVETLKLSEDDAIFPENAAYFIAMGAALYSTKLGTMLFD
                     DLLDHLENAKEVKTISNAQEPLFDTQDEYERFRQRHSQARAEYQDLTDYRGDAYLGID
                     AGSTTTKLVLINKKGDILFQYYAPNKGNPVAVVKDQLSEIYARCGDRVQIRGSAVTGY
                     GEDLIKNAFCVDAGLVETVAHYYAARHFKPEVDFILDIGGQDIKCFKIRNNAVDSIML
                     NEACSSGCGSFIETFASALGYSVEEFAKLGLFAKHPVDLGSRCTVFMNSSVKQAQKEG
                     AGVDDISAGLAMSVVKNAIYKVIRAANAAELGRHIVVQGGTFYNDAVLRSFEKEFGFE
                     VTRPVISGIMGAYGAALYATELEAEPGLISEKELGNFVHASKAASCGLCTNRCPLTVN
                     TFGGGRKFISGNRCERPLGRKIREGLPNLYKYKYEKLNSYIPLKNAPRGKIGIPRTLN
                     MYENLPFWHTFFTNLGFEVVLSPESTRSMYIKGQYTIPSDTVCYPAKIVHGHIEALLE
                     QGLDVIFYPCMSYNFDEKRSDNHYNCPVVAYYPELVAANIGNLKKIRYLNPYFGLHRP
                     RDFEKKAAEYFGREFGIPAKETKLAARAAYRAYHEYEADIRRQGEAALEFAEKNEKRV
                     IVLAGRPYHIDPEINHGIDRLISSYDMVIVSEDSVAHLVEPAKVRVLNQWTYHARLYS
                     AAKFATMHKNTELIQMVSFGCGIDAITGDETRAICEKGGKLYTQIKIDEINNLGAVKI
                     RIRSLIAAMDSRNKENG"
     gene            2798..>3640
                     /locus_tag="VHR42_08095"
     CDS             2798..>3640
                     /locus_tag="VHR42_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HEX3027171.1"
                     /translation="MAQLKYSKDGRLLFTKEMKKDYKILMPMMLPIHFTLIRNSFRLE
                     GYDIELLSTNHRAIVDEGLKNVHNDTCYPALLVIGQFIDALKSGKYDVDKTALIITQT
                     GGGCRASNYIHLLRKALKRSGYEKVPVISLNISGLESNPGFKLRYNLVRRMIYSLLYG
                     DLIMLLANQCRPYEAAQGATDRLVDQWINRLTEDYKNPKNLKYKNVLKTMKLIAAEFA
                     SLKLEGDPKIRVGVVGEIYIKYAPLGNNNLEQFLATEGAETVLLLPDYRQVCLRAFSN
                     QNCFA"
BASE COUNT          910 a          879 c          992 g          859 t
ORIGIN      
        1 agtcgatccg agaaaattat gccggaagac cggttcacgt ttcggttacc ggatccgcgg
       61 gccttggcgt ggcgaaggtc tgcggactgg aatttgtcca ggaagtgttt gcaacggccg
      121 gggcggtgga agcctacctg cccggaaccg acgcggtgat tgagctgggc ggggaggacg
      181 cgaagatcat tttctttacc ggcgggcttg aagagagaat gaatggctcc tgtgccggcg
      241 ggaccggtgc gtttatcgac cagatggcga cgcttctggg cgtttccacc gacgagctgg
      301 atgcacttgc ggccaaacat gagaaaatct attccattgc gtcccgctgc ggcgtgtttg
      361 cgaaatcaga tattcaaccg ctgctcaatc agggggcgcg caaagaggat attgcagcgt
      421 ctgtttttca ggccgtggtc gagcagacca tcgccggcct tgcgcaggga cgaaaaattg
      481 aggggaaagt ggcgttcctc ggtgggccgc tttctttttt cagcgtcttg cgtgagagat
      541 ttgtcgagac cctgaagctt tcggaagacg acgcaatttt tccggaaaac gcggcgtatt
      601 ttattgcgat gggcgccgcg ctgtattcca ccaaacttgg gacgatgctg tttgacgatt
      661 tgcttgacca tcttgaaaat gccaaagagg tcaaaacaat ctccaatgcg caggagccgc
      721 tgtttgatac ccaggatgaa tatgagcggt tccgccaaag acattcccag gccagagccg
      781 agtatcagga tctgacggat taccgggggg acgcctatct tggcatcgac gctggaagca
      841 cgacgaccaa gctcgtcttg atcaataaaa agggagacat cctttttcaa tattacgcgc
      901 cgaacaaggg caaccctgtg gcggttgtca aagaccagct gagtgagatc tatgcccgct
      961 gcggcgaccg ggttcaaatt cgcggtagcg cggttaccgg ttacggcgag gatctgatca
     1021 aaaatgcttt ttgtgtggac gccggccttg tggaaacggt agcccattat tacgcggccc
     1081 gtcatttcaa gcccgaggtg gatttcattc tcgatatcgg cggtcaggac attaaatgtt
     1141 ttaagattcg caacaacgcg gttgattcca tcatgctgaa tgaagcgtgt tcctccggct
     1201 gcggctcgtt tatcgagacg tttgcctcgg cgcttggtta ctcggtggag gaattcgcaa
     1261 aactcggatt gttcgccaaa catcctgtcg atcttggctc gagatgtacg gtgtttatga
     1321 attcttcggt caagcaggcc cagaaggagg gcgccggtgt cgacgatatc tccgcggggc
     1381 ttgccatgag cgttgtgaag aatgcaatct ataaagtcat ccgggcggcc aacgccgccg
     1441 agctgggcag gcatattgtc gtccagggcg gaacttttta caatgacgcg gttctccgca
     1501 gctttgaaaa ggaatttggc tttgaagtga cgcgtcctgt tatttccggt atcatgggcg
     1561 catacggcgc ggctctttac gctacggaat tggaggccga gccgggtctg atctcagaaa
     1621 aagaactcgg gaattttgtg catgcatcca aagcggccag ctgcgggttg tgcaccaacc
     1681 gatgtccgct gaccgtcaac acgttcgggg gaggacggaa attcatcagc ggcaaccgct
     1741 gcgagcggcc gcttggaagg aagatccggg aaggccttcc caacctttat aagtataaat
     1801 atgaaaaatt aaactcatac atacctttaa aaaatgcgcc ccgcggaaag attggcatcc
     1861 caaggacgct gaacatgtat gagaacctgc ctttctggca tacgtttttc accaatctcg
     1921 gatttgaagt ggttctttct cctgaatcga cccgtagcat gtatatcaag gggcagtaca
     1981 cgattccatc cgatacggta tgctatccgg cgaaaattgt gcatggccat atcgaagcgc
     2041 ttctcgagca aggccttgat gtcattttct atccgtgtat gtcgtataat tttgatgaaa
     2101 agcggtccga taaccattac aactgcccgg ttgtagccta ttatcccgag cttgtggccg
     2161 ccaatatcgg caacctgaaa aaaatccgtt atctcaatcc ctatttcggc cttcaccgtc
     2221 cgcgtgactt tgaaaaaaag gccgccgagt atttcggcag ggaattcggc atacccgcaa
     2281 aagaaaccaa gcttgccgca agagcggcct atcgcgcata tcacgaatat gaggccgata
     2341 ttcgcagaca gggcgaagcg gcgctggagt ttgcggagaa aaatgagaaa cgggtcattg
     2401 tcctggccgg gagaccctat catatcgatc cggagatcaa tcacggaatc gacaggctta
     2461 tctcgtccta cgacatggtg attgtgtcgg aggattccgt cgctcatctg gttgagccgg
     2521 caaaagtcag ggtgctcaat cagtggacct atcatgccag actttattcc gcggccaaat
     2581 ttgcaaccat gcacaaaaac acagagctga tccagatggt ttccttcggc tgtgggatcg
     2641 acgcgatcac cggggatgaa acccgcgcaa tctgtgaaaa gggcggaaaa ctttacaccc
     2701 agatcaaaat tgatgaaatc aataatctcg gagcggtgaa aatcaggatt cgaagcctca
     2761 ttgccgcgat ggattcgaga aataaggaga acggatgatg gcgcaactca aatattcaaa
     2821 agacggcaga cttcttttta caaaggaaat gaaaaaggac tataaaattc ttatgccgat
     2881 gatgctgcct atccatttta ccctgatccg caacagcttt cggctggagg gctacgatat
     2941 cgagcttctt tccaccaacc accgtgcgat cgtcgacgag ggcctgaaaa atgttcataa
     3001 cgatacctgc tacccggcgc ttcttgtcat cgggcagttt atcgacgcgc tgaaaagcgg
     3061 taaatatgac gtggacaaaa ccgcgctgat tatcactcag accggcggcg gctgcagggc
     3121 ttcaaactat attcacctgc ttcggaaggc gctcaagcgc agcgggtatg aaaaagttcc
     3181 ggtgatttca ctcaatattt cagggcttga gagcaacccg ggtttcaaac tgcgttacaa
     3241 tttagtccgc aggatgattt acagcctgct ttacggcgat ctgatcatgc tgctggctaa
     3301 tcagtgccgc ccttatgaag ctgcgcaggg tgcgaccgac cggctggttg atcagtggat
     3361 caaccgtctg accgaagact ataagaatcc gaaaaattta aaatataaaa atgttctgaa
     3421 aaccatgaaa ttaatcgcgg cggaatttgc ctcgctcaaa ctcgaaggcg atccaaaaat
     3481 acgggtgggc gttgtaggcg agatttatat taaatacgcg cccctgggca ataataacct
     3541 cgagcagttt ttggcaacgg aaggagcgga aacggtatta ttgttgccgg attatcggca
     3601 ggtatgctta cgggcctttt caaatcagaa ttgtttcgca
//
LOCUS       DATALY010000299         8604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_581998,
            whole genome shotgun sequence.
ACCESSION   DATALY010000299 DATALY010000000
VERSION     DATALY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581998"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VHR42_08100"
     CDS             <1..1261
                     /locus_tag="VHR42_08100"
                     /inference="COORDINATES: protein motif:HMM:NF017502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sip1-related alpha-galactosidase"
                     /protein_id="HEX3027172.1"
                     /translation="DADEMRFPEGIGGVIRHLKEDYGIRWVGVWQAVMGYWNGVDPDS
                     DAYREFSEYLEILPSGKILPRTDTAYSFGFWNKWHQYLRSCGVDFIKVDSQSSISLFQ
                     EGRHTYGQAASGVHTGLEASAALNFGGNLINCMGMAPEDIWNRPQAALSRNSDDYVPK
                     VPHAIREHALQNAYNSLLHGNFYWEDWDMFWSGHEAAGQNAILRALSGGPIYLSEPIG
                     ATKPENIFPLILNDGRILRCESVGLPTQDCLLRDPVEGTAPLKIFNHCRENYAVGVFN
                     ISNAEQTETAELSAEDIPALRDKSLWVYDWQNRCASTLKEKEHFSVDVEPDGARLFLL
                     FPRTEEPAIVGLMQKYLSFAAVNWVEKFGPEYRIQIPQGGTFAFLSKHQPTAVLLDGS
                     TVTPRVKGELYQIECSEESAHRIQICM"
     gene            1433..1843
                     /locus_tag="VHR42_08105"
     CDS             1433..1843
                     /locus_tag="VHR42_08105"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3027173.1"
                     /translation="MIQPICRDTFILSRKSIPATKADLPVVEDLLDTLKANADQCVGM
                     AANMIGANKRIIVFSIGEMNIPMMNPVIIKREDSFETEEGCLSLDGMRKTTRYRTVEV
                     EFLNQKFERKKQTFTGFPAQIIQHEIDHCDGILI"
     gene            complement(2012..3202)
                     /locus_tag="VHR42_08110"
     CDS             complement(2012..3202)
                     /locus_tag="VHR42_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3027174.1"
                     /translation="MHAIYWSICCSSSNFASVFLLSKNFSNRQIGFILASANILAAFL
                     QPAIADIADKNRTVTLKDLTVLLTGAASLFAAARFFTFGCSVVIAVLLILELTLLLVL
                     QPLVTSLGMQRINSGITVNFSLARGIGSMTFAFFSVILGYLIGCFGTGLLPALSAVLY
                     VLQIIAINAFAGKQALTKSNPPETNRCTAKASSAGASTDNLIVFMAQNKRFVALVAAV
                     IFTFCSHSMINNYFIQITENVGGKTKEMGIAIGLAAAIELPAMVLFNGFTKHIPCSTI
                     LKFSLFFFLVKAVVTLYASNIWLLYAAQLLEFFSYALFVPASIYYVNRIIREEDRTKG
                     QAFITSAITIGSVVASFLGGWLLDCRSVKIMLLVGTFFAALGFLIGLGAVEKRKKHDF
                     SATS"
     gene            complement(3297..3968)
                     /locus_tag="VHR42_08115"
     CDS             complement(3297..3968)
                     /locus_tag="VHR42_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX3027175.1"
                     /translation="MEAIKQQLQMVIFDLDGVIVDSEPLHDAAKKWILEQNGVMEKPN
                     LSWSVGIPGVDIWTRLIQQYGLRAAPAEFERQQYEFILSRIRENHIPESHGLKELLAW
                     LRSRNITVGLASSSDRFYVERILKHYELTDRFQFVTAGDEVPQKKPAPDVYRNDLSRS
                     GIHAGQAVAIEDSASGIQAAAAAGMYCIGYRNPTSGNQDLSKADWIVNSLADVPKVLL
                     DSFTF"
     gene            complement(4016..5137)
                     /locus_tag="VHR42_08120"
     CDS             complement(4016..5137)
                     /locus_tag="VHR42_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX3027176.1"
                     /translation="MKSVNGFEIKKLNRSRIYNQIRKQGSLSRQDLVAALRLSLPTVT
                     QNLKELQDEGLLCESGSFGNTGGRHARAYSITQDARIAVGIDITRHRIALVAVNLEGR
                     VLSQRQVSCNFSLDNAYFRGLGSEVENLIRESDFDPQKVLGVGIAVPGLITEDCQKVF
                     YGEILNFTSATNDAIGRYIPYPHALYNDANAAGFAEIWSNPEIKDAFYIMLGNNIGGS
                     ILINHCVYSGDGSRSGEVGHITLSPDGPLCYCGQRGCFETYCNATVLSTHTDGDLNAF
                     FDRLKNGDPECRDVWQQYLRYLSIAVNNVCMLFNCRVILGGYVGAYLDDSVDELKALC
                     AKRNPFEKDADYIQVCKYKTEAIAAGAALPYIDQFIGSI"
     gene            5476..6357
                     /locus_tag="VHR42_08125"
     CDS             5476..6357
                     /locus_tag="VHR42_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3027177.1"
                     /translation="MRSAKMKIRPYLMVAPAMAGLLLFTVYPIFRLIQLSLTDVNMLD
                     RTKSKFIGFRNFQQIFVRPDFRQALSNTFVYTVAVVFLITALSLVFAVWLNSTKSRLN
                     NLLQGMIFFPNIVSIVSVSLVWLWMMEPTYGLFNYVLRSLSLPTLQWLSSSQTAMPSV
                     ILVSVWKSVGYDTLIYLAALQSVSPSIYEAASLDCKNKFTIFFKITFPMISPLLFFTL
                     IIRVIDSFKMFDTVRVMTEGGPNNATMTLVYEIYNEALQNVRIGYSSAIGVVLLVIIS
                     VLTLGYFKLYAKKVHYQ"
     gene            6375..7199
                     /locus_tag="VHR42_08130"
     CDS             6375..7199
                     /locus_tag="VHR42_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3027178.1"
                     /translation="MKALNRPIGWLVKGILLVLFGWPFFWMISTSLQTISEVNTVTPT
                     FLPAIPQFGNYIKAWNFGATGMNGFLKNSLLTIVAVISLQTLVMVPAAYAFAKYEFRF
                     KKIMFGCILAALMLPTQITFLPVYLMMSNWNLINTLWPQILPFATNAFAIFLLRQYFL
                     QIPNELIDAARIDGASETRIICGLMLPMSKPALATITLFSFVGHWNDYFWPFVMTNSD
                     STRTLPVAIAQMKDLEGLSNWNVVMAGNALLVLPILLIYLVAHKQIVKSFAYSGIK"
     gene            7246..8544
                     /locus_tag="VHR42_08135"
     CDS             7246..8544
                     /locus_tag="VHR42_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3027179.1"
                     /translation="MKKFLSICVSLSLLAATFTGCASSTPAAMASAGNSGGGTSGKIT
                     VQFWHCMSGDNGDLTNKLVDDFNKSQDKIKVVATYQGSYSDAAAKAEQAIYAGNGPDL
                     LQVAQDNVGRLATNGQFTDLLPYVKKAGINPDSFTKAFVQDAYYDGKLVDIPYGRSAQ
                     LLHINKTVLDQVGAKIPTTWDELKEAAEKCTVQKNGKITRYGLSIPFDQWELFALIQQ
                     AGGQFFNKDKTGLGCLDDGSAKKSFQFLKSMQKNGALFYNDPANDKDNQLFTQGMAAM
                     SINSSGTITSRTKSIGSKFNYVVVPLPKDVKYSMPTGGSGFGILSSSKQKDAAWQFMQ
                     WFVQNEKGGTAFVLGSGYLPFTQKMTQSKAIQDLWKKIPNYKVAYDALQYGDDSYRIA
                     NLTPVIAEFRTCIQAIMLDNKDVDTSLKTFSDSVNTILAK"
BASE COUNT         2251 a         2122 c         2060 g         2171 t
ORIGIN      
        1 ggatgcggat gaaatgcgct tcccggaagg gatcggcggc gttatccggc atttgaaaga
       61 ggattacggc atccgttggg tcggagtttg gcaggctgtt atgggttatt ggaacggggt
      121 agacccggac agtgatgcgt accgcgagtt ttccgaatac cttgagattc ttcccagcgg
      181 taaaatcctg ccgcgcacgg atacggcgta cagctttggt ttctggaata aatggcatca
      241 gtaccttcga agctgcggcg ttgattttat caaggttgac tcacaaagct ccatttctct
      301 gttccaggag ggacgccata cctacgggca ggccgcaagc ggtgtgcaca ccgggctgga
      361 agcctctgcc gccctgaatt tcggcggcaa tctcattaac tgcatgggta tggcgcccga
      421 ggatatctgg aaccgtcctc aggcggcgct ttcccgcaat agcgacgact atgtgccaaa
      481 agttccccac gccattcggg aacacgctct ccaaaatgcc tacaactcgc ttctgcacgg
      541 gaatttttac tgggaagact gggacatgtt ctggagcggc catgaagcgg ccggacaaaa
      601 cgcgattctg cgggcgctaa gcggcggccc gatttacctg agcgaaccga ttggagcaac
      661 gaagccggag aatatattcc cgctgattct caatgacggc cgcattctgc ggtgtgaaag
      721 cgttggcctg ccaacgcagg actgtctgct ccgtgacccg gtagaaggga cagctccgct
      781 gaaaatcttt aaccattgca gggagaacta tgcggtcggt gtgtttaata tcagcaatgc
      841 ggagcaaacc gaaacggcgg agcttagcgc ggaggatatt ccggcgctgc gggacaaatc
      901 cctctgggtg tacgattggc aaaaccgctg cgcatccact ctgaaagaaa aggaacactt
      961 ttctgtcgat gtggaaccgg acggcgccag actgttcttg cttttcccaa gaacggaaga
     1021 gcctgcaatt gtcggtctga tgcagaaata tttatctttt gccgccgtga attgggttga
     1081 aaagttcggc cccgagtatc ggattcagat tcctcagggc ggtacgttcg ctttcctctc
     1141 aaaacatcag ccgacagcag ttctgcttga cggctccact gttactccgc gggtgaaggg
     1201 ggaactttac caaatcgagt gctccgagga gagcgcccac cggattcaaa tttgcatgta
     1261 agatgttaac ggaagaaata aagaagccga gaaagtgata ctatagatag gtgccgcgag
     1321 caccggattc ctaatgttct acctatccga tgaagctcgg ctccgaacca aactgcgaag
     1381 gcaaatgcgc caactaaaaa tttccgtaaa attcagataa gacggtgaga gtatgattca
     1441 accaatatgc agagatacat ttattctaag cagaaaatct attccggcca ccaaggcgga
     1501 tctgccggtg gtcgaggatc ttttggatac gctgaaagcg aacgcagatc agtgtgttgg
     1561 tatggccgct aatatgatcg gcgccaataa gcgcattatc gtgttcagta ttggagaaat
     1621 gaacattcct atgatgaatc cggtcatcat caagcgggag gattcctttg aaaccgaaga
     1681 aggatgcctg tctttggacg gcatgcggaa aaccacccgc taccggaccg ttgaagtgga
     1741 gttcctcaac caaaaatttg agcggaaaaa acagaccttt accggtttcc ctgcgcagat
     1801 catacagcat gaaatcgatc actgcgatgg aatcctgatt tgagttttga ttcattcaat
     1861 tgcgggcgtt tcaatatcag cgaaccgtga gtgaagcgtc aaggaatgca gaaccggact
     1921 tttcgcaaac ttatgctgat tgtaaaaaac aaataagtag gtaaagaaag aacccagtag
     1981 ccgcatggct gctgggttct gcacattttg tttacgatgt tgcgctgaaa tcgtgtttct
     2041 tccgcttttc aacagccccc aaaccgatca gaaatcccaa agcggcaaaa aacgttccaa
     2101 ccagaagcat gatttttaca ctgcggcaat ccaaaagcca gccgcctaaa aaacttgcca
     2161 ctacgctgcc gattgttata gcgcttgtaa taaaagcctg ccctttcgtt cgatcttctt
     2221 cccttatgat tctgttaaca taatagattg aggcggggac aaacagcgca taggagaaaa
     2281 actccagcaa ctgtgcggca tacagcagcc agatatttga agcatacaag gttacaacag
     2341 ccttcaccag aaaaaagaac agtgaaaatt tcagaatggt actgcacggg atatgttttg
     2401 tgaaaccgtt aaacaaaacc attgccggca attcaatcgc ggctgcaagt ccgatggcaa
     2461 ttcccatttc tttcgtttta ccgccgacat tttctgtaat ctgaatgaaa taattattga
     2521 tcatggaatg ggaacagaat gtaaagatta cggcagcgac cagtgcaaca aatcgtttgt
     2581 tctgcgccat aaacacaata agattatccg ttgatgcgcc tgccgaggag gctttggccg
     2641 tacagcggtt tgtctccggg ggattgctct tggtcagggc ttgctttccg gcaaaggcgt
     2701 taattgcgat aatctgcagc acataaagca cggcggataa tgctggaagc aaacccgtgc
     2761 cgaaacagcc gattagatat cccaaaatga cggagaaaaa agcaaaggtc attgagccga
     2821 tcccccgtgc aagactgaag ttaacggtaa tcccgctgtt aatccgctgc attccaagcg
     2881 aagtcaccaa aggctgtagg accagaagaa gcgtcagttc cagaatcaac agcactgcaa
     2941 tcacaacaga acagccaaac gtaaaaaatc gggcagctgc gaaaaggctg gccgctccgg
     3001 taagaagaac cgtaagatct ttcagggtta ccgttctgtt tttatcggcg atatccgcga
     3061 tcgcaggctg cagaaacgcc gcaagaatat tcgctgacgc aaggataaag ccgatttgcc
     3121 ggttgctgaa atttttggac agcaaaaaga ccgatgcaaa atttgaactg ctgcagcaga
     3181 tcgaccaata aatcgcatgc acataggaat agttccgggt gtatgtattc ttcgtaccta
     3241 tcgttttaat ttcatgaacc gcctttacga atcaatttgc aaaacaggga agggtatcaa
     3301 aacgtaaagg aatccaacag aaccttcgga acatctgcca gactgtttac gatccaatcc
     3361 gccttcgaaa ggtcctgatt tcccgaagtg ggattacgat agccaatgca gtacattccc
     3421 gcagcggctg cggcctgaat tcccgacgcg gagtcttcaa ttgcaaccgc ctgcccggca
     3481 tgaatcccgg aacgactcag atcgtttcgg taaacatccg gcgcgggctt tttttgaggc
     3541 acttcatctc cggctgttac gaattgaaac cggtcggtca gttcatagtg cttcaatatg
     3601 cgctctacat aaaaccgatc ggaagaggat gcgaggccaa cggtgatatt cctgcttctc
     3661 agccacgcaa gcagttcttt cagaccgtgg gattccggta tatggttttc acgaatgcgg
     3721 gaaaggataa actcatattg ctggcgttca aattcagcgg gagcggctct cagcccgtac
     3781 tgctgaatca gcctcgtcca gatatccaca cccgggattc cgaccgacca ggaaaggttc
     3841 ggcttttcca taacgccgtt ttgctccaga atccatttct ttgccgcatc atggagcggc
     3901 tcactgtcaa caattactcc gtccagatca aaaatcacca tttgcagctg ttgcttgatt
     3961 gcttccatta ttttatcatc cgtttcctaa aatgtatgcg gcgcggagag cacctttaaa
     4021 tcgaaccgat aaattgatcg atatacggca gagcagcgcc tgctgcgatc gcttcggttt
     4081 tgtatttgca cacctgaata taatccgcat ccttttcaaa aggatttcgc tttgcgcaca
     4141 gggctttcag ttcatcgaca gaatcgtcga gataggcgcc gacatacccg cccagaatga
     4201 cccggcaatt gaaaagcatg cagacgttgt ttaccgcgat ggacaggtat cgtaaatact
     4261 gttgccaaac atcgcggcac tccgggtcgc cgttcttcaa tcggtcgaaa aaagcgttca
     4321 aatcgccgtc ggtatgggtg gaaagtaccg ttgcgttgca atatgtttca aaacagccgc
     4381 gttgcccgca gtaacagagt ggaccgtccg gcgagagggt gatatgcccc acttcgccgc
     4441 ttcgcgaacc gtcgccgctg tagacgcagt ggttaataag aatcgagccg ccgatattgt
     4501 tgccaagcat gatataaaac gcatccttaa tttcggggtt cgaccatatt tctgcaaagc
     4561 cagccgcgtt cgcgtcgtta tacagagcat gcggataagg aatgtaccgt ccgattgcat
     4621 cgttcgtagc gctggtaaaa ttcagaattt caccgtagaa aaccttctgg cagtcttccg
     4681 ttatcaggcc gggaacggca atgcccaccc cgagtacctt ttgcggatcg aaatccgatt
     4741 cccggatcag attttccact tcgctgccga gtccgcgaaa ataggcattg tccagactga
     4801 aattgcagga tacctgtctt tgactcagga ccctgccttc cagatttacc gcgaccagcg
     4861 cgatccggtg ccttgtaatg tcaattccca cggcaatgcg cgcgtcttgt gtgatggaat
     4921 atgcgcgcgc gtgccggcct ccggtgtttc caaagcttcc gctctcgcaa agcagaccct
     4981 cgtcctgaag ctctttgaga ttctgggtaa cggttggtag gctgaggcgc agggcggcga
     5041 ccagatcctg tcgggaaaga gaaccctgct ttcgaatctg attatagatt ctgcttcggt
     5101 tcagcttttt aatttcaaaa ccgttgacac ttttcatacc caaccgtcat ccttaatgtt
     5161 ttataaaata actttctata atttgaattt attataacag gtttttcaat atatttcaac
     5221 gtaatacata aatcaactta tcgaataaag catctaaaat tttgcttatt taataaataa
     5281 ttctatttta agcaacttga tggaattata tatactcaat tatataaaat gactttccaa
     5341 atattacatc attttaaatt ttacttaaag tcttcttaac aagatacctc tatactgaat
     5401 ctctgttatg taaagttatt ttacataatt catcaagctt tattagctgc gaagttttgt
     5461 tcagggggta cttacatgag aagcgcaaaa atgaagattc ggccttacct gatggtggcg
     5521 ccggcaatgg ccggactact gctgtttacc gtatatccga tttttcgatt gatccagctc
     5581 agcctgacgg atgttaatat gctggacagg acaaaaagca aattcattgg ttttcggaat
     5641 ttccaacaga tttttgtccg gccggatttc aggcaggcac tttccaacac atttgtgtac
     5701 acggtcgctg tggtgttcct gatcaccgct ctttctcttg tgtttgccgt gtggcttaac
     5761 tcgacaaaaa gccgtctgaa caaccttttg cagggaatga tcttctttcc caatattgtt
     5821 tccatcgttt ctgtttccct ggtctggctg tggatgatgg aacccactta cgggcttttc
     5881 aattatgtgc ttcgttccct cagcctgccc acgctgcagt ggctttccag ttcgcagact
     5941 gcgatgccgt cggtaatact cgtatccgtc tggaaaagcg tcggctatga taccttgatt
     6001 tatctcgctg ctttgcaaag cgtatctccc agcatttacg aggccgcgtc actggactgc
     6061 aaaaataagt ttacgatctt ctttaaaatc acgtttccca tgatttcgcc tctgctgttc
     6121 tttaccctga ttatccgcgt gattgactcg tttaaaatgt tcgataccgt ccgggtgatg
     6181 acggaaggcg gaccgaacaa cgccactatg acgttggtat acgaaattta caacgaggcg
     6241 ctgcagaacg tgagaatcgg atacagctcg gcaatcggag ttgtgcttct tgtcatcatt
     6301 tcggtgctga cgcttggtta ctttaaactg tatgcgaaaa aagtgcatta ccaataaggg
     6361 gggagggaaa accgatgaaa gcactcaatc gaccgatcgg ctggctggtc aagggaattc
     6421 tgctcgtcct gtttggctgg ccgtttttct ggatgatttc gacgtcgctg cagacaattt
     6481 cggaagttaa cacggtaacg cctaccttcc tgccggccat acctcagttt gggaactaca
     6541 taaaggcatg gaactttggg gccacgggaa tgaatgggtt cctgaagaat tcgcttctga
     6601 ccattgttgc cgtcatttcg cttcaaacac ttgtaatggt ccctgctgct tacgcgttcg
     6661 caaaatatga gttccgattc aaaaaaatca tgttcggatg catccttgcg gccttaatgt
     6721 tgccaacgca gatcacgttc cttccggttt acctcatgat gtcgaattgg aacctgatca
     6781 acacgctctg gccgcagatt cttccgtttg ccaccaatgc gttcgccatc tttttactgc
     6841 gccagtattt tttgcagatt ccgaacgagc tgatcgatgc cgcgcggatt gacggtgctt
     6901 cggaaacgcg tatcatctgt ggactgatgc tccccatgtc aaaaccggcg ttggcaacaa
     6961 tcacgctgtt cagctttgtc gggcattgga acgattattt ttggcccttc gttatgacaa
     7021 attccgacag cacgcgcact cttccggttg ccattgccca aatgaaagat ctggagggtc
     7081 tgtccaactg gaatgtcgtc atggcgggca acgcgcttct tgtccttcca attctgctga
     7141 tttacctcgt tgcgcataaa cagatcgtca agtcctttgc ctattccggc attaaataaa
     7201 aaagcaggtc actcacgact ttgctgctac ttcaaggagg catttatgaa aaagttccta
     7261 tccatctgcg tctcactctc gctgcttgcg gccacgttca cgggctgcgc atcttccacc
     7321 cccgccgcaa tggcgtccgc cggaaattca ggaggcggaa catcggggaa aattaccgtt
     7381 cagttctggc actgtatgtc aggagacaac ggcgacctca ccaacaagct ggtggatgac
     7441 tttaataaat ctcaggataa gataaaggtt gtggccacct atcagggcag ctattcagat
     7501 gccgccgcaa aagcagagca ggccatttac gcggggaacg gcccggatct tctgcaggtt
     7561 gcccaggaca acgtcggccg ccttgctaca aacggacagt tcaccgacct tcttccctac
     7621 gtgaaaaaag ccggcatcaa tcccgacagc tttaccaaag cattcgtgca ggatgcgtat
     7681 tacgacggaa aactggtcga tattccttat ggccgttcgg cacagctgct gcacatcaac
     7741 aaaacggttc tggatcaggt gggcgcgaaa atccccacta cttgggatga gctgaaagaa
     7801 gcggctgaga aatgcaccgt tcagaagaat gggaaaatta cccgctacgg tctgtcgatt
     7861 ccgttcgatc agtgggaact gtttgccctc attcagcagg ctggcggtca gttctttaat
     7921 aaagacaaaa cggggctcgg ctgcctggac gatggaagcg ccaaaaaatc ttttcagttt
     7981 ttgaagagca tgcagaaaaa cggcgctctg ttttacaacg acccggcaaa tgataaagac
     8041 aaccagcttt ttacccaggg catggccgcc atgtccatca attcatccgg aaccatcact
     8101 tcccgcacga aaagcattgg cagcaaattc aattatgttg tcgttcctct tccgaaagac
     8161 gtgaagtact cgatgcctac gggcggcagc ggcttcggaa ttctctcctc cagcaaacag
     8221 aaagacgccg catggcagtt catgcagtgg tttgttcaga atgaaaaagg cggaaccgca
     8281 ttcgttcttg gctcgggcta cctgccgttt acgcagaaaa tgacgcagtc caaagcaatt
     8341 caggacctgt ggaaaaagat ccccaactat aaagtggcat atgacgcgct tcaatatgga
     8401 gacgacagct accgcatcgc caacctgacc cctgttatcg cggaattccg cacctgcatt
     8461 caggcgatta tgctcgataa caaggatgtt gacacttctt taaaaacatt cagcgattcc
     8521 gtcaatacca tccttgcaaa gtaagtattg aaaaaaagca ggaaaagaga ttagactatg
     8581 tggaatcaga ccgaaaaaaa tatc
//
LOCUS       DATALY010000300        10574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_410552,
            whole genome shotgun sequence.
ACCESSION   DATALY010000300 DATALY010000000
VERSION     DATALY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410552"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="gtfA"
                     /locus_tag="VHR42_08140"
     CDS             complement(<1..874)
                     /gene="gtfA"
                     /locus_tag="VHR42_08140"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEX3027180.1"
                     /translation="MKRNNKIMLITYSDSIGANLKELSAVLNRYYSKAIGGVHILPFY
                     PSSADRGFAPMTYREVDPAFGNWEDVEALARNYYLMFDYMINHISRNSEYFQDFLKRK
                     DASPYQDLFIRYQDFWPGGDATQEDIDRIYKRKPRPPYMEAEFANGTREKVWCTFDEE
                     QIDLNLPSETTKAFNRENLRFLTERGAALIRLDALAYATKKAGTDCFFVEPNIWDILK
                     ECDRTLQPLNVELLPEIHEHYRYQMKLSEHGYWVYDFALPMLVLYSLYSGKKNRLEHW
                     LGICPRKQFTTLDTH"
     gene            complement(892..1869)
                     /locus_tag="VHR42_08145"
     CDS             complement(892..1869)
                     /locus_tag="VHR42_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3027181.1"
                     /translation="MANIKDVAKLAGISVTTVSRVLNNKGYISEKTRSSVQKAMEELN
                     YQPNEVARTLFSRQSHLLGLIIPTVAHPFFSQLTRYIEFYASQKGYKVILCNSLMQKE
                     KEKEYLDMLGRHQVDGIIMGSHTLDVGNFKTIGLPVITLDRQISEKIPFVSSDNYSGG
                     ILAAEHLIAKGCRHLAHISGNLNLSMLSNRRYDGFKSVCEREHVAYILYQTDANALSR
                     ENQSALVEKIFREHPETDGVFASSDLLAAEVLKNCLKHGRKIPEEIKIVGYDDTDLTR
                     YFYPSITSVAQPIESIAKYTVDYLIAEVMGEVVPNQTILEVSLSERETT"
     gene            complement(1939..2763)
                     /locus_tag="VHR42_08150"
     CDS             complement(1939..2763)
                     /locus_tag="VHR42_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3027182.1"
                     /translation="MNGKRIFVSVIRAVVAVAVAALFAFPLVFLVINSFRDKMDIVDN
                     PLSFAGNLSLENFFNAIDRMQYLSSFVNSLIVTVCSAVVIMFFASMLAFFIARFETRL
                     SKGIFYTLVVSMIVPFQAVMIPFVYIYGQLHLLNSIPALVFFYLGFGLSLTTFMYHGF
                     IKRLSVSLEEAASLEGASLFTIFWKVVFPQLVPITSTTFVLNVLWFWNDYLLPSLVLI
                     QKSRTLPLATYTFFGTFTSDYGIAMAGLMLSIIPIIIFYVFMQKNIVGGITDGAVK"
     gene            complement(2764..3642)
                     /locus_tag="VHR42_08155"
     CDS             complement(2764..3642)
                     /locus_tag="VHR42_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3027183.1"
                     /translation="MYSNTKLKQRVKYFLIFAAFPLFAFLVVLLIPFLLGAALSFTNW
                     NGVTFTKWIGVQNFVNAFQDARFVNSMLLTFQYVLVSVILVNVVGFGLALLVTSKIHC
                     KNLLRSIFFVPNLLGGVLLGFIWQFIFGRVLVYFGGATGIPLFSDSWLVDPQMAFWAM
                     VVVTIWQQAGYMMLIYIAGIVGISNDMIEAASIDGASSLRQLFQIKMPLMAQSFTICL
                     FLTLKNAFMMFDVNLALTKGGPYNATELITLNIYNDAFLNQNYGTAQAKAIVFFLIVS
                     VIALCQVAISKRKELD"
     gene            complement(3836..5176)
                     /locus_tag="VHR42_08160"
     CDS             complement(3836..5176)
                     /locus_tag="VHR42_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3027184.1"
                     /translation="MLKKLIALILACAMVSSLAACSGGSSTSSAGSESSKAAGSAAAQ
                     TGGKTKITILGTLKDEVAEPFKQEMAEYNKSQDKYEISSIPLDGTAMQKLTTLYASGN
                     APTIFTSGQEASTFKDKLMDLSKVDQIKQAQAGTTELMTDKSGKVFGMPLTVEAFGIL
                     YNKKVLDKAVGGTFDPSTINTQDALKDLLAKVAKTGVSADRVSGVDWSLGAHMTNLMF
                     TTQNNSLDKNLQFMDDLKNGKVDLASNKVYNGWLDTVDLLLANNASKKSPLAPTYDDD
                     ILAFAKEKTGLWFQGNWAFPMIQKANSSADVGIMPFPISNNPSDYGNTQISVGASQYF
                     CVDASQSTKEQQAGALDFINWFFTSKTGQDYYVNKLNFIPAYKNITATPSDNLSKMVV
                     KYMNDNKTLSWINMYYPGDGVTSLGAIMQKYVAGKADRKELAKELQTYWTSKKS"
     gene            5528..7192
                     /locus_tag="VHR42_08165"
     CDS             5528..7192
                     /locus_tag="VHR42_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX3027185.1"
                     /translation="MTNQWWKNSVVYQIYPKSFLDSNGDGVGDLNGITQRMDYIASLG
                     VDVVWLCPVFQSPMDDNGYDISDYYKIDPLFGTNEDMDRLLAEAKARGIKVLLDLVVN
                     HCSDEHAWFQRAKSNPCCEEAGYFYFAKTSDGKEPNNWRSSFGGSVWSQLPDGRWYYH
                     TFSPKQPDLNWENPKLRREVYKIIEYWLQKGAAGFRVDAITFIKKELSFQSREPEEGG
                     RYSVEHLTNLPGIGAFLEEMRTEVFEPYNCMTVAEAPGVDMASFRRYAGNDGYFSMIF
                     DLNWDDMEGEPDKSNLAAVERWKEKIFRSQRFTSASGWPAVFLENHDQARCVNKFLEC
                     KDRSFFSASALATIYFFLYGTPFIYQGQEIGMTNAVWNSADEMDDVRVRRALEEKTYA
                     EEPPEKLLEQLSELSRDHARTPMQWNAGPNAGFSTGTPWLKVQENHTEINVENAESDP
                     SSLLSYYKKLVYLRRNADCSNIFRDGAFEPLMEDRHGIIAYRRFVGDASVYVAVNFTN
                     TPKEAALPGGVCLLSNHREPVSDSRSMILKPYQAVVFYDTRSKARE"
     gene            7210..10245
                     /locus_tag="VHR42_08170"
     CDS             7210..10245
                     /locus_tag="VHR42_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX3027186.1"
                     /translation="MKIEHYFQNLEILHVNTENDRSYYIPFPEHSEIFPEIREKSSRF
                     QLLNGEWRFRFYENMESVPQDFPTGAAFEKDCPTLTVPSAWQMHGYDRNQYTNMRYPF
                     PFDPPFVPVQNPCGAYVTDFEISQDRAPFRKYLNFEGVDSCCYVWVNGRFVGYHQVSH
                     CTGEYDITDYVSPGSNRLAVLVLKWCDGSYLEDQDKLRMSGIFRDVYLLYRPQNFIRD
                     YFIRTETENFSNDGPAHTSVEVAVQFHGAKEPLIYCLFAPDGTLAAQGESDDDNFSVQ
                     LENPKFWNAEQPLLYTLLLTSCGETLQEKVGIRTVRVENGVLQVNGKPVKIKGVNRHD
                     SNPFHGCAVTEEDIRTDLTLMKRHNINAIRTSHYPNPPILPRLCDEYGFYLIAEADLE
                     AHGQEMIYRPTEEDLCFFANDPAYRESILDRVKKLVQRDKNRPCILFWSLGNESGYGP
                     NFEDSARWVRDFDSSRLIHYEHIAHVMPGSSPDFSLLDVTSRMYPPTEFIGEYFQDAQ
                     KSTPKPLLLCEYCHAMGNGPGDLEDYFSLVYRYDGFAGGLIWEWCDHSVYDGKAENGK
                     DRYLYGGDFQDEPNDGNFCLDGLVTPDRKPSTGLLEYKNVIRPVRAETDSDEAWKFRL
                     TNCLDFTDTGDAVRIQYEITCDGAVAEQAELPELRIPPHGSQTVQIQPKREYDGVVLL
                     RFRYLQKVGTALVPDGEELGSDQFRISDPQVLYRQHPDLSAAARQAADFTETESAVTI
                     KGQDFTYTFDKDTGLFRGMTFRDAELLLRPMEYNLWRAPTDNDQFIRTEWEQAGYDCV
                     QPFVYELSVAQEANCVVIRTQTGLVPVYRQRILKIFSEFRICSDGRVTASMNVEKNPV
                     FPFLPRFGVRLFLPKSFENLSYFGYGPYESYIDKHRASYLGLFCGEADEAFEDYIKPQ
                     ESGSHYGCEYLKVSSAQGASLTVQNEDRFCFNLSHYTEEELTQKRHSFELEASDAAVL
                     CIDYKQSGIGSNSCGPELLEQYRLHENQFSFHFEVSPSL"
     gene            complement(10367..>10574)
                     /locus_tag="VHR42_08175"
     CDS             complement(10367..>10574)
                     /locus_tag="VHR42_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3027187.1"
                     /translation="LRDINYWRKIVNSSRDAEVLSVYEMESNENVWMDWLSQENEIAV
                     GDRKTMNAGGGKYLNFIAFVLRKK"
BASE COUNT         2648 a         2732 c         2665 g         2529 t
ORIGIN      
        1 cgtgggtatc cagcgtggta aactgctttc tggggcagat tcctagccag tgttccaacc
       61 ggtttttctt tccggaataa agggaataca gtaccagcat cggcaaagca aaatcataaa
      121 cccaatagcc gtgctcggaa agcttcatct gatagcggta atgctcgtga atttcaggaa
      181 gtagctccac atttagtggc tggagggtac ggtcgcattc tttcagaatg tcccatatgt
      241 tcggttctac aaaaaagcaa tcagtacccg cctttttggt cgcgtaagcg agggcatcca
      301 gccgaatgag tgcagctccc cgctccgtca gaaaacgcag attttcacgg ttgaacgctt
      361 tggttgtctc actggggaga ttcaggtcaa tctgctcttc gtcaaatgtg caccagactt
      421 tctcccgcgt gccattcgca aattccgctt ccatatatgg tgggcggggc ttacgcttgt
      481 aaatgcggtc gatgtcttct tgcgtagcat cgccgcccgg ccaaaagtcc tgataccgga
      541 tgaaaaggtc ctgataaggg gaagcgtctt tccgcttcag aaaatcctga aaatattcgc
      601 tgtttctgga aatatggttg atcatatagt caaacatcag atagtaatta cgcgcaagcg
      661 cttccacgtc ctcccagttt ccaaaggccg ggtcgacttc acgataggtc atgggtgcaa
      721 agccacggtc cgcggaggag ggataaaacg gaagaatatg caccccgccg atcgctttgg
      781 aataataccg gttcagaacg gcggaaagtt ccttcaggtt agcgccgata ctgtctgagt
      841 aagtaatcag catgattttg ttgtttcgtt tcatcttggt ttaaaattcc tttatgttgt
      901 ttctcgttcg cttaaagaga cttccagtat cgtctggttt ggcaccacct cacccatcac
      961 ctcggcaatc agataatcca ccgtgtattt cgcaatgctt tcaatcggct gcgccacgga
     1021 ggtaatgctc gggtaaaaat accgggtcag gtcggtatcg tcgtatccga cgatttttat
     1081 ctcttcggga atctttctcc cgtgcttcaa acagtttttt agaacctcgg cagcaagcag
     1141 gtcgctgctt gcaaaaacgc cgtctgtttc cgggtgctcc cggaagattt tttccaccag
     1201 tgcggattgg ttttcgcggc tcagagcgtt cgcatcggtc tgatacagaa tataggcgac
     1261 gtgctcccgc tcgcatacgc ttttaaagcc gtcgtaccgc cggttggaaa gcatgctcag
     1321 gttcaagttt ccgctgatgt gagcaaggtg gcggcagccc tttgcaatca ggtgctccgc
     1381 cgcaagaatt ccgccggagt agttatcgga ggaaacgaag gggatctttt cggaaatttg
     1441 ccggtccagc gtaataacgg gcagaccgat cgttttaaaa ttgccaacgt ccagcgtatg
     1501 actacccatg atgatgccgt ccacctgatg gcggccgagc atatcgaggt attccttttc
     1561 cttttccttt tgcatcagcg aattacacag aattaccttg taacccttct gtgaggcgta
     1621 aaattcgatg tatctggtaa gctgcgaaaa aaacggatgc gcaaccgtag gaataatcag
     1681 tcccaataaa tgtgattgcc ttgaaaacag agttcgcgcc acctcattcg gctggtaatt
     1741 gagttcctcc atcgcctttt gcaccgaact gcgcgtcttt tcactaatat agcccttatt
     1801 gttcagtacc cgcgacaccg tcgtgacgga aatacctgcc agtttagcca catctttaat
     1861 attggccaat cattccaccc cattttatag taatgcgtcc ccacgctgaa gaattttgtt
     1921 catgtaaaac aactttctct attttaccgc accatcggta ataccaccaa caatattttt
     1981 ctgcatgaat acatagaaaa taatgatcgg aatgatgctg agcatcagcc ccgccatggc
     2041 gattccgtaa tcgcttgtga aagtgccaaa aaaggtatag gttgcgagcg gaagcgttct
     2101 ggatttctga atcagcacca gagaaggaag caggtaatcg ttccagaacc acagaacatt
     2161 cagcacaaaa gtggtcgacg taatgggaac cagctgcggg aaaaccactt tccagaaaat
     2221 ggtgaacagc gaagccccct ccagactggc cgcctcctcc aaggaaacgg acaggcgttt
     2281 aatgaacccg tgatacataa acgtggtcag gctcaggcca aatccgaggt aaaagaaaac
     2341 cagcgccgga atgctgttga gcagatggag ttggccgtag atgtagacaa acgggatcat
     2401 gacggcctgg aacggcacga tcatggaaac caccagcgtg tagaaaatcc ccttgcttag
     2461 cctggtttca aacctagcga taaagaacgc cagcatcgag gcaaaaaaca taataaccac
     2521 agcgctgcag accgttacaa tcagcgagtt tacgaacgaa ctcaggtact gcatgcggtc
     2581 aatggcgtta aaaaagttct ccagtgacag attgcccgca aaggaaagag ggttgtcgac
     2641 gatgtccatt ttatcgcgga aggaattgat caccagaaaa accaggggga aagcaaacag
     2701 cgccgcaaca gcgacggcaa cgaccgcccg gatcactgaa acgaaaatgc gtttcccgtt
     2761 catttaatcc aattcctttc tttttgaaat tgcgacctga cacagtgcga tgacgctgac
     2821 gatcaggaag aaaacaattg cctttgcctg cgcggtcccg taattctgat ttaaaaaagc
     2881 gtcgttgtaa atattcagcg tgatcagttc ggtggcgttg tagggtccgc ctttggtcag
     2941 cgcaaggttc acatcgaaca tcataaacgc gtttttcagc gtcaggaaca ggcaaatggt
     3001 gaacgactgc gccatcagtg gcattttaat ctgaaaaagc tgccgcaggg aggaagctcc
     3061 gtcgatggat gccgcttcga tcatatcgtt ggagataccc acaataccag caatgtagat
     3121 cagcatcata tagcccgcct gctgccagat ggtgacaacc accatggccc agaacgccat
     3181 ctgcggatca acgagccacg aatcactgaa cagcgggatc ccggtagccc cgccaaaata
     3241 caccagtacg cggccgaaaa tgaactgcca gataaagccc agcagaacgc cgcccaacag
     3301 attcggcaca aagaaaattg agcgcagcag atttttgcag tggattttcg acgttacaag
     3361 aagcgccaga ccgaacccca ccacgttaac gagtatgacg gaaaccaaaa cgtattggaa
     3421 ggtaagcagc atactgttga caaatctggc gtcctgaaat gcgttcacaa aattttgcac
     3481 accgatccac ttcgtaaatg taacgccgtt ccagttggtg aaggaaagcg ccgctcccaa
     3541 cagaaaagga atcagcagca ccactaaaaa agcgaacagc ggaaatgctg caaaaatcaa
     3601 aaagtacttg actctttgtt tgagcttggt atttgaatac acaccgcatc ctcctacttt
     3661 tcatgatatt ctcagcacgt tccttttcat tgcggcggcg gttcaggcga aacgctaagc
     3721 ggaaaagaat cggcgtttgg ttgcttattc aagaatgccg gcctaaccag cccacagtgg
     3781 cgaaagcact gtctggttag gccggaacat aaccggttat aaaagggtaa gatgtttacg
     3841 attttttgct ggtccagtag gtctgcagct ctttcgcaag ttccttgcgg tcagccttgc
     3901 cggctacata cttttgcatg atcgcgccca gcgaggtaac accgtcgccc ggatagtaca
     3961 tgttaatcca gcttagggtt ttgttgtcgt tcatgtactt gacaaccatc ttggaaaggt
     4021 tatccgaagg agttgcggtg atgttcttat aagccgggat aaagttgagc ttgttcacat
     4081 aataatcctg gccggttttg gaagtgaaga accagttgat gaagtcgaga gcgcccgcct
     4141 gctgttcctt cgtggactgc gaagcgtcaa cgcagaaata ctgagaagca ccgacggaaa
     4201 tctgggtatt gccgtaatcg gacgggttat tgctgatcgg gaacggcata atccccacat
     4261 cggcggagga gtttgctttc tgaatcatcg ggaacgccca gttgccctgg aaccagaggc
     4321 cggtcttctc ttttgcgaag gcgagaatgt cgtcgtcata ggtcggagca agcggggatt
     4381 tcttggaagc attgttggcc aaaagcagat cgacggtgtc gagccagccg ttatagacct
     4441 tgttgctcgc aagatcgacc ttgccgtttt tcaggtcatc catgaactga aggttcttat
     4501 cgagcgaatt gttctgggtg gtgaacatca ggttcgtcat atgtgcgccc aaagaccagt
     4561 caacaccgga aacgcgatcc gcgctgacgc cggtctttgc gaccttggcc agcagatcct
     4621 tcagagcgtc ctgtgtgttg atggtggaag gatcaaaggt gccaccgaca gccttgtcga
     4681 gcaccttctt attgtaaagg atgccgaaag cttccacggt gagcggcatg ccgaaaacct
     4741 tgccgctttt atcggtcatc agttcggtcg taccggcctg tgcctgcttg atctgatcaa
     4801 ccttgctcag gtccatcagc ttgtccttaa aggtgctggc ttcctgaccg gaagtaaaaa
     4861 ttgtaggagc gtttccgctt gcgtacagcg tcgtcagctt ctgcatggcc gttccatcca
     4921 gagggatgga agaaatttca tacttgtcct ggctcttatt gtattccgcc atctcctgct
     4981 taaagggttc ggcaacctca tctttaagcg taccgagaat ggtaattttc gtctttccgc
     5041 cggtctgtgc cgctgcgctt ccggcagcct tggagctttc ggagcccgcg gaactcgtgg
     5101 agcttccgcc ggagcaggcc gcaagagacg ataccatcgc gcaggccaaa atcagtgcga
     5161 tcagtttttt caacattatt ctcctctttc ctgatgtgtt ttcttcgttg caaggcaggt
     5221 ttgtgataaa gaattatggc aactgcagtt ccataaggcg tagacacagt gtaacactaa
     5281 tcatttttaa tgtcaaccgg tttcatattc attatttttt tgtgtagatt tttggcctat
     5341 gttcttatat aatttgcaat ttgtcaatga atagtgctat aaataatata tatacagccc
     5401 tattattgca caaattcaat atgaatccgt ttccattttc tcttgcaccc aaactggcga
     5461 aatgctataa tacccatgaa aacacattcc ctttctttgg gtatatccgc agtgaatgga
     5521 gataagcatg acaaatcaat ggtggaaaaa ctcggttgtc tatcaaattt accccaaaag
     5581 cttcctggat tccaacgggg acggagtcgg cgatctaaac ggaattacgc agcggatgga
     5641 ttacatcgca tccctcggcg ttgacgttgt gtggctttgc ccagtatttc agtcgcccat
     5701 ggacgacaac ggatacgaca tttccgacta ttataaaatc gaccccctgt tcggaacaaa
     5761 cgaggacatg gaccggcttc tcgcggaggc caaagcacgc gggattaagg tcctgcttga
     5821 tcttgtagtc aatcactgct cggacgaaca cgcctggttt caaagagcga aaagcaatcc
     5881 atgctgtgag gaggccggtt atttctattt tgccaaaaca agtgacggga aagagcccaa
     5941 caactggcgt tccagcttcg gcggttccgt ctggagccag ctgccggacg gccggtggta
     6001 ttaccacacg ttttcgccca agcagccgga cctgaactgg gaaaacccaa agctgcgccg
     6061 ggaagtttac aaaatcattg aatattggct gcaaaagggc gctgccggat ttcgcgtgga
     6121 tgcaatcacc tttattaaaa aagaactgtc gtttcagtcc cgcgagccgg aagagggcgg
     6181 ccggtactcg gtggaacatc tcaccaatct gcccggaatc ggcgcgtttt tggaagaaat
     6241 gcgcacggaa gtttttgaac catacaactg catgacggta gcagaggccc cgggcgtaga
     6301 catggcatcc ttccgtcggt acgccggaaa tgacggctat ttttccatga ttttcgattt
     6361 aaactgggac gacatggagg gagaaccgga taaatccaat cttgccgccg tggaacggtg
     6421 gaaagaaaaa atattccgca gtcaacgctt taccagcgcg agcggctggc ccgccgtctt
     6481 tttggaaaac cacgatcagg cccgctgcgt caacaaattt ctggaatgca aagaccgcag
     6541 cttcttcagt gcctccgccc tggcaaccat ctacttcttc ctgtacggga cgccgtttat
     6601 ttatcagggg caggaaatcg ggatgacgaa cgccgtatgg aacagcgcag acgaaatgga
     6661 cgacgtacgt gtccgtcgtg ctctggaaga aaaaacatac gcggaggaac cgcccgagaa
     6721 actgctggaa cagctgagcg aactgagccg tgaccatgca aggacgccca tgcagtggaa
     6781 cgccggaccc aacgccggat tcagcaccgg caccccctgg ctgaaagtgc aggagaacca
     6841 tacggaaatc aacgtggaaa acgcggaaag cgacccttcc tctctgcttt cttactacaa
     6901 aaagctggtc taccttcgcc gcaatgccga ctgcagcaat attttccgcg acggcgcatt
     6961 tgagccgctg atggaggatc gccacgggat aatcgcttac cggcgattcg ttggggatgc
     7021 ttcggtgtat gtcgctgtca atttcaccaa tactccgaaa gaagcagccc tgccgggcgg
     7081 ggtctgcctg ctgtcaaatc accgggaacc cgtatcggac agccgttcca tgatccttaa
     7141 accgtatcaa gccgtggtat tttacgacac tcgcagcaag gcgcgggaat aaaaaagagg
     7201 agatcgcaga tgaaaatcga gcactatttt cagaatttgg agattctgca tgtcaatacg
     7261 gaaaacgacc gctcctatta tatccctttc ccggagcatt ctgaaatctt tccggaaata
     7321 cgggaaaaat caagccgctt tcaactgctg aacggggaat ggcggttccg gttttacgag
     7381 aatatggaat cggttccaca ggattttccc acgggagctg cctttgaaaa agactgccct
     7441 actttgactg tgccgtccgc gtggcagatg cacggctacg accggaacca atacaccaac
     7501 atgcgttacc cgttcccgtt cgacccgccg tttgttcccg tgcagaaccc ctgcggtgct
     7561 tatgtgaccg attttgaaat ttcacaggac cgggccccgt tccgcaaata tctgaatttt
     7621 gagggcgtgg actcctgctg ctacgtctgg gtaaacggcc gctttgtggg ctaccatcag
     7681 gtttcccact gcacgggcga atacgacatt acggattatg tctcccccgg ctccaaccgt
     7741 ctggcggttc tggtgctgaa atggtgcgac ggctcttact tagaggatca ggataagctc
     7801 cgaatgtccg gcatcttccg cgacgtttat ctgctttacc gcccgcagaa ctttatccgg
     7861 gattatttta ttcgtacaga gacggagaac ttttccaacg acggcccagc acacacttct
     7921 gtggaggttg ccgtgcagtt ccatggtgcg aaagaaccgc ttatttactg cctgtttgca
     7981 cccgacggca cgcttgccgc acagggagaa tccgacgacg ataatttttc ggtgcaactt
     8041 gaaaacccca agttctggaa cgcggaacag ccgctgcttt acacgctgct tttaacatcc
     8101 tgcggtgaaa ctcttcaaga aaaagtcggc atccgcacag tgcgggtgga aaacggcgtt
     8161 cttcaggtaa acggcaaacc ggtgaaaatc aagggagtga atcggcacga cagcaacccg
     8221 tttcacggct gcgcagttac cgaagaggat atccgtaccg atttaaccct gatgaaacgc
     8281 cacaacatca acgcgatccg caccagccat taccccaatc ccccgattct gcctcgtctg
     8341 tgcgacgaat acggctttta cctgattgcc gaggcggatt tggaagcgca cgggcaggaa
     8401 atgatctacc gcccaaccga agaagacctg tgcttctttg cgaacgaccc cgcataccgg
     8461 gaaagcattc tggaccgggt gaaaaagctg gtacagcggg ataaaaacag gccttgcatc
     8521 ctattctggt cgctgggcaa cgagtcgggt tatggcccca atttcgagga ctccgcccgc
     8581 tgggtgcggg actttgacag ttcccgactg attcactatg agcatatcgc tcacgtcatg
     8641 cccggctcca gcccggactt ttccctgctg gacgtcacca gccgcatgta tccgccgacc
     8701 gaatttatcg gggaatattt tcaggacgcg cagaaaagca ctccaaagcc gctccttctg
     8761 tgtgaatact gccatgccat gggcaacggg ccgggcgacc tggaagacta tttctcgctg
     8821 gtttaccggt atgacggctt tgccggcggg ctgatctggg aatggtgtga ccattcggta
     8881 tatgacggga aggcggaaaa tggaaaagac cgttacctct acggcggcga ttttcaggac
     8941 gaaccgaacg acggaaactt ctgtctggat gggcttgtca caccagaccg gaagccttcc
     9001 accggactgc tggaatataa aaacgtaatc cgtccggtcc gtgctgagac cgactcggac
     9061 gaagcctgga aattccggct caccaactgt ctggatttta cggatactgg ggacgccgtg
     9121 cgcattcagt atgaaatcac ctgtgacggt gcagtggcgg agcaggccga gctccccgaa
     9181 ctccgcattc ctccgcacgg gtcccaaacg gtgcagattc agccaaaacg ggagtatgac
     9241 ggcgtggtgc tgctccgctt ccgttacctg caaaaggtcg gcacagcact ggttccggac
     9301 ggcgaggagc tcggctctga tcagttccgg atcagcgatc cgcaggttct gtaccgtcag
     9361 catcccgatc tttccgccgc agcacggcaa gcggcagact ttacggagac ggaatccgcc
     9421 gtcaccataa aggggcagga ttttacctat acattcgata aagacacagg gctgtttcgc
     9481 gggatgacct tccgtgatgc agaattgctc ctacggccca tggagtataa tctttggcgg
     9541 gcgccgaccg acaatgacca gtttatccgc acggaatggg agcaggccgg atacgactgc
     9601 gtacagcctt ttgtctatga attgagtgtg gcacaagagg cgaattgtgt ggtgattcgg
     9661 acacagaccg gactcgttcc cgtctaccgc cagcgcattc tgaagatttt ctcggaattc
     9721 cgcatctgct cggacggccg ggtgaccgct tccatgaatg tggaaaaaaa tccggtattt
     9781 ccgtttcttc cgcgcttcgg agtgcgcctg tttctgccaa agtcatttga aaatctgtcc
     9841 tatttcgggt atggcccata cgaaagctac atcgacaaac atcgtgcgtc ctatctggga
     9901 ctgttctgcg gcgaggcaga cgaagcgttt gaggattata tcaagccgca ggaaagcggc
     9961 tctcattacg gatgtgaata cttaaaggtt tcctccgcgc agggggcttc cctcaccgta
    10021 cagaatgaag atcgtttttg ctttaacctc tctcattaca cggaagaaga gctgacacaa
    10081 aaacgacaca gcttcgaact ggaagcatcg gacgccgccg tactatgcat tgattacaag
    10141 caaagcggaa tcggctccaa cagctgcggg ccggagcttc tggaacagta ccgactgcat
    10201 gaaaatcagt tttcattcca ttttgaggtt tctccctcgc tctgataaaa cgaccctggc
    10261 tgaatcaaga atacggaaga gcggcaatca tccgttgttc cttagctaga gggagcccgg
    10321 cagccataaa gctgccgggc tctctctgcc ttacttctgg atctctttat tttttccgca
    10381 ggacaaaagc gatgaaattc agatattttc cgccgcccgc attcatggtt ttgcggtcac
    10441 ccaccgcaat ctcattttcc tggctcagcc aatccatcca aacgttttcg ttgctttcca
    10501 tctcatatac ggaaagaact tcggcatctc ggctgctgtt cacgattttc ctccagtaat
    10561 tgatatcccg caga
//
LOCUS       DATALY010000301         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_754156,
            whole genome shotgun sequence.
ACCESSION   DATALY010000301 DATALY010000000
VERSION     DATALY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754156"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="VHR42_08180"
     CDS             <1..1843
                     /locus_tag="VHR42_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027188.1"
                     /translation="FISVILGLLIVYANQFLIKRRIKELGVYMTLGMEKGKISRILIL
                     ETFLIGIFALAVGLLVGVFASQGLSVVTAKMFEVSLKAFLFTFSIKAFYKTLIYFSII
                     FVIVMIFNTVSISKLKLIDLLTAGKKNENLKIKKLWISVVLFLVAVVCLGFAYYFINK
                     NGMLMWNTMFAASIILGCIGTLLFFLSLSGFLLRVVQSNKRLYFKNMNMFVLRQINSK
                     INTTFVSMTCICIMLLITIGTLSTGIGLANTLSDSVRKSTPYDITFSYESRKDVPERS
                     YIDLVKALAKDNIDLNRYAKDYVQLNIYTSDLDYGGLKLDDSNIRQSYGDEVADGFKK
                     TPIPVITLSEYNKAAQMQGKPTLALKENEFAVNCTMEQFQLGLEAFCRGEGKITLNGK
                     QLTCASKTFSQTALMTSMSQTDSGTVIVPDALVKGLKFYGAALNVNYKSSAVDAEFRQ
                     LLDTVYGSKSAKSKSCPYDMALSKSHIYEQGAGLRTAVCYIAIYIGLVFLITSAAVLA
                     LQQLSEASDNTERYSLLRKLGTDEKMINRALFGQIGIYFFIPLSLAIVHSIVGIRVAN
                     NVVAILGHIDIVGNSAFTAAIFLLIYGGYFLATYFGSKSMIQQRQNG"
BASE COUNT          474 a          458 c          461 g          542 t
ORIGIN      
        1 tttcatttcg gtaattctag gcttactgat cgtgtacgcc aatcagttcc tgatcaagcg
       61 ccgtataaag gaactcggcg tctatatgac gctcggcatg gagaaaggaa aaatatcccg
      121 catcctcatt ctggaaacct ttcttatcgg catatttgcc cttgccgttg gtctgctggt
      181 cggcgttttt gcctcccagg gattaagcgt agtgactgca aaaatgtttg aggtcagcct
      241 gaaagcgttt ctctttacct tttccatcaa ggctttttat aaaacgctga tttatttcag
      301 cattattttc gtgattgtta tgatattcaa tacggtctcc atttccaagc tcaaactcat
      361 cgatttgctg accgccggaa aaaagaacga aaacctgaaa atcaagaagc tgtggatttc
      421 ggtggttctg ttccttgtgg cggtcgtttg cctcggcttt gcctattact ttatcaataa
      481 aaacgggatg ctgatgtgga acaccatgtt tgccgcgtcc attattctgg gctgcatcgg
      541 gacgctgctg tttttcctct ccctttccgg ctttctgctg cgcgttgtcc agtcaaacaa
      601 gcggctttac ttcaaaaata tgaatatgtt cgtgctgcgg cagatcaaca gcaagatcaa
      661 tacgaccttc gtttccatga cctgcatctg cattatgctg ctgattacca tcggcaccct
      721 gtccaccggc atcgggctgg cgaacaccct ttctgacagc gtgcggaaat ccacgcctta
      781 cgatattacc ttttcctatg agagccggaa agacgtccct gaaagatcat atattgatct
      841 tgtaaaagct ctggcaaagg ataacattga cctgaaccgt tacgcaaagg attatgttca
      901 gctgaatatc tatacttccg atttggatta cggcggcttg aagcttgacg attcgaatat
      961 tcggcagagc tacggcgatg aagtggcgga tggattcaaa aagacgccga ttccggtgat
     1021 tactctttcc gaatataaca aggcggctca aatgcagggc aagccgacgc tagctttgaa
     1081 ggaaaacgaa tttgccgtaa actgcactat ggagcagttt cagttgggtt tggaagcctt
     1141 ttgccgcggc gaaggaaaaa tcaccctcaa cgggaagcag ctgacctgtg cttcaaaaac
     1201 ctttagccag accgccttaa tgacttccat gtcgcagacg gattccggca cggtaattgt
     1261 tccggacgca ctggtaaagg gcttgaagtt ttacggtgcg gcgcttaatg taaattataa
     1321 atcaagcgcg gtcgatgcgg agttccgcca gcttcttgat acggtttatg gcagcaaatc
     1381 agcaaaaagc aaaagctgcc cttatgatat ggcattgtca aaaagccata tctatgagca
     1441 gggcgcaggt ctgcgcacgg cggtatgcta tatcgccatc tatatcggcc ttgtgttcct
     1501 gatcaccagc gccgcggtgc tcgccttgca gcagctctcc gaagcgtcgg acaatacgga
     1561 gcgttacagt ctgctccgca agctcggcac ggatgaaaaa atgattaacc gcgcgctgtt
     1621 cggtcagatc gggatttatt tcttcatccc actgtcgctt gccattgtgc attccattgt
     1681 aggaatacgc gtggcaaaca atgtggtggc gattctcggg catatcgata ttgtaggcaa
     1741 ttcggccttt accgcggcaa ttttccttct catttacgga ggctatttcc tcgcaacata
     1801 ttttggcagc aagagcatga tacagcagag gcagaatgga tgaattatga taattttgat
     1861 cctgatgaaa tggttcttac tgtatgggtt cctgattggg tttgttaagc catagtcaat
     1921 ctaagccgcc gtccc
//
LOCUS       DATALY010000302         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_672090,
            whole genome shotgun sequence.
ACCESSION   DATALY010000302 DATALY010000000
VERSION     DATALY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672090"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="dnaJ"
                     /locus_tag="VHR42_08185"
     CDS             <1..1040
                     /gene="dnaJ"
                     /locus_tag="VHR42_08185"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX3027189.1"
                     /translation="HPDLHPGDKQAEAKFKEVNEAYEVLSNPEKRSRYDQFGQAGVDP
                     NFNPGAGAGGFDFNDFGDIFSNLGDIFGFGGTSQRRNGPVRGGDIHVHVALSFEEAAK
                     GSKKQIETYRMQSCEACGGSGAKKGTSAETGPSCHGAGTVKVSQRTPFGIVSTSRTCE
                     RCGGRGKIVKTPCPECNGTGVYRKSRKLEVDIPAGIDDGQVITLRGQGDAGRNGGPSG
                     DVDISVSVRPHPIFTRNGYDVWCDLPITFVQATLGCEVTVPTLYGKVSYNVPAGTQPG
                     DKFRLKGKGIQQLNRYGKGDQYVKIVVEIPKNLNDKQKQILRQFEESTGEGCYEKRKS
                     FFKKLKDAMGL"
     gene            1118..2104
                     /gene="prmA"
                     /locus_tag="VHR42_08190"
     CDS             1118..2104
                     /gene="prmA"
                     /locus_tag="VHR42_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3027190.1"
                     /translation="MDWIETTIFTTTEGIEPVTGRLYRLGVQGVQIADESEFNEFLDN
                     NRQYWDYVDEKLREKMVGETRVTVYLSDNASGHEMLTAVRNSIHELRNMDKEHIFGRL
                     AIETNGLKEEDWTENWKRYFKPLKIGKKILIQPAWEKLSPENRDRTVFSVEPGMVFGT
                     GAHESTRLCIEASEKYVQQGSSALDLGCGSGILSIIALLLGAKSAVAVDIDPNAVDVA
                     YANAARNGIGKDRYTVFSGDILADEAVRKRIGGARYDVVFANIVADVILVMVPLIPSL
                     LQPGGVFITSGIITERRDEVAEALLAQGFEIEESNEEKGWAELTCRLKPAHS"
     gene            2118..2840
                     /locus_tag="VHR42_08195"
     CDS             2118..2840
                     /locus_tag="VHR42_08195"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX3027191.1"
                     /translation="MPRFFTKDIDENFARITGQDAKHISRVLRMKPGEKLTVCDGMGN
                     DYLCEITSMLEHEVTLSILDKSKSKSEPAVSVTLFQGLPKSDKMDYIVQKSVELGITR
                     VVPVVTARSVSRPDEKSMHGKIERWNKIAAEAAKQCGRGQIPKVETTVTFHRALDELR
                     RMETALLFYEGSTAKIAGQLNNTVREIGFLIGPEGGLEPGEVQAAKEAGIFVAGLGPR
                     ILRTETAPICVLSIIMYATGNL"
     gene            complement(2915..4018)
                     /locus_tag="VHR42_08200"
     CDS             complement(2915..4018)
                     /locus_tag="VHR42_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3027192.1"
                     /translation="MIRTIVVDDEWYNLEEICDMVEKTGFMTVRSRCQNGADALREAA
                     RVLPQAAFIDIEMPEMDGLTLAEKLLEAYPEMKIVFITGWNQYAVAAFELNALDYIMK
                     PLNKARFQKMAERLKSQFKTNETEEESALTIRCFGGFEAVMNGRPVIWKRLKAEELFA
                     YLLLHQGIFVNKDIILENLWPDYEYTKSLPILQTSVCKIRNIFSDFRDEVKLIYADNK
                     YGLFLSGVQCDYLDTEDALARFQGNMPETYNAVESACEIFQKGLFASCGYLWSEGLQE
                     NLRQRLTSALRIIARAYRIQPDNTCGLETLKQLSRLLPEDEDAQLLYLNTLRVFGKKE
                     QIAQHGKWLCETLKADYGAELPEAIDEIIKEQI"
     gene            complement(4021..>5212)
                     /locus_tag="VHR42_08205"
     CDS             complement(4021..>5212)
                     /locus_tag="VHR42_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX3027193.1"
                     /translation="IRGYLESVRMYRGSYLLMILASFLPWIAVYFNIRNSTFLRIDYY
                     SLMMIGTGILYLFGLFRYDIFNTVPIATETVYRLSGDAIALIDNSGRITDVNAAFLKL
                     YPEMKRLTVKSTQNDFLARHHELRGLSPEKSKIRFYKEDNQLKRYFSAELIGIQTESG
                     IPIGNIVQIKDISLFVKYQNQLKQLAEQANERAETNELSFLQAQISPHFINNTLSAIC
                     SLISRDDDAAKDLVVNLSEYLISCYRIGSSSPMDTLEHELEAVDTYVQIVYARFGGRI
                     RYTAEVTAPLALELPRLILQPLVENAIRHGLQPKKDGGTVRLSVETDGECARFEISDD
                     GVGIEPARIPSLLSGEDDKQGVGMINIHKRLLKYYGEGLNIKSGSGTSVSFRIPLTKA
                     VREG"
BASE COUNT         1309 a         1222 c         1397 g         1284 t
ORIGIN      
        1 accatccgga tctccatcca ggcgataaac aggcggaagc aaaattcaag gaagtcaacg
       61 aagcatatga ggtgctgtcg aatccggaga aacgttccag gtatgaccag tttggccagg
      121 ccggggttga tccgaatttc aatcccggag caggtgccgg cgggtttgat ttcaatgatt
      181 tcggggacat tttttcaaac ctcggcgata tcttcggctt tgggggaacg agccagcgca
      241 gaaacggccc ggttcgcggc ggagatattc atgtacatgt ggcgctttct tttgaggaag
      301 cggcaaaagg cagcaaaaag caaattgaga cttaccggat gcagagctgc gaagcatgtg
      361 gaggaagcgg cgcgaaaaaa ggaaccagcg ccgaaaccgg cccgtcgtgt catggagccg
      421 gtacggtaaa agtgagccag cgcacgccgt ttgggatcgt ttcgacttcc cgtacgtgtg
      481 aacgctgcgg tgggcgcgga aaaatcgtta aaactccctg ccccgaatgc aacggaaccg
      541 gcgtttaccg gaaaagccgg aaacttgaag tcgatatccc ggctggtatc gacgacggac
      601 aggtcattac gctgcgaggg cagggggacg cgggccgcaa cggggggcct tccggcgacg
      661 ttgacatttc cgtgtccgtc cgcccccatc cgatttttac aaggaacgga tacgacgttt
      721 ggtgcgatct gcccatcacg ttcgtgcagg ctacgctcgg atgcgaagtt accgtaccga
      781 cgctttacgg aaaagtcagt tacaacgttc cggcaggcac acagccgggg gataaattcc
      841 gcctgaaagg gaaaggcatc cagcagctaa atcgttatgg gaagggcgac caatacgtta
      901 aaatcgtagt ggaaatcccg aaaaatctta atgataaaca gaaacagatc ctgcggcagt
      961 ttgaagaatc taccggggaa ggctgttacg aaaaacgtaa atcctttttt aaaaaactaa
     1021 aagacgccat gggtttatga ccctggcgtt ttctcatact gttcgcttga ttaatggcaa
     1081 acaagattcg gtaaaactgt tgggggttaa aaagtaaatg gactggattg agacgacgat
     1141 tttcacgacg acggagggca ttgagcctgt gacggggaga ctctaccgct tgggggttca
     1201 gggcgttcag attgcagacg agagcgagtt caatgagttt ttggataaca accggcaata
     1261 ctgggattac gtagatgaaa aactgcgcga aaaaatggtg ggagaaaccc gcgttaccgt
     1321 ttatctgagt gacaatgcct caggacatga aatgctgacc gctgttcgga acagcattca
     1381 tgagctccgt aacatggata aggaacatat ttttggccgg cttgcgatcg aaacgaacgg
     1441 cctgaaggaa gaggactgga cggaaaattg gaagcggtat tttaaaccat taaaaattgg
     1501 gaaaaagatt ttgattcagc ctgcatggga aaaattatcg ccggaaaaca gggataggac
     1561 ggttttctcc gttgagcctg ggatggtttt cggcaccgga gcacatgaat ccaccagact
     1621 ttgcattgaa gcctccgaaa agtatgttca gcagggaagc agtgcgctgg atctcggctg
     1681 cggcagcgga attttatcca ttattgcttt actgctgggc gcaaaaagcg ccgtcgctgt
     1741 ggacattgac ccaaatgcgg ttgacgttgc ttatgcgaac gcggcacgga acggaatagg
     1801 aaaggaccgg tatacggtgt tttccgggga tattctggcg gacgaagccg ttcgaaaacg
     1861 aatcggcggg gcccgttacg acgtagtatt tgccaatatc gtggcggacg tcattttggt
     1921 tatggttccc ctgatcccca gtctgctgca gcccggcggg gtttttatca catccggcat
     1981 tattaccgag cgcagggatg aagttgcgga ggccctgctc gcacagggat ttgaaatcga
     2041 agagtcaaat gaggaaaaag gctgggcgga actgacttgc aggctgaaac ccgcccactc
     2101 ctgaaggagg ctgtcttgtg cccaggtttt ttacgaagga tattgatgaa aattttgcgc
     2161 gtattacagg ccaggatgcg aaacatatct cccgcgttct gcggatgaaa cccggtgaaa
     2221 agcttacggt ttgcgacggc atgggaaacg attacctttg cgaaattaca agtatgctgg
     2281 aacacgaggt aacgcttagt attttggata aaagcaaatc aaaatccgag cctgccgtca
     2341 gcgtaacgct gtttcaggga cttcccaaat ccgataaaat ggattatatc gtgcaaaaat
     2401 cggtagagct tggcataacc cgcgttgtcc ctgttgtcac cgcgcgaagc gtgtcgcggc
     2461 cggacgagaa gtccatgcat ggaaaaatag aacgctggaa taaaattgcc gcggaagccg
     2521 ccaaacagtg cggccgcggt caaattccga aggttgaaac cacggtgaca tttcatcggg
     2581 cgctggatga gcttcgccgg atggaaactg cactattgtt ttacgaggga agtacggcga
     2641 aaattgccgg gcagctgaat aatacagtcc gggaaatcgg tttcttgatc ggtccggaag
     2701 gcggtttgga acccggcgaa gtacaggccg ctaaggaagc gggaattttc gttgccggtc
     2761 tcggcccccg gattctgcga actgaaaccg ccccgatttg cgtattgtca ataatcatgt
     2821 acgcaaccgg aaatttataa tctttcagac atttttttat tgtttgaaaa gtcagctcct
     2881 ttttaaaaac tagagtatgt attcgggatc atttttaaat ctgctctttt ataatttcat
     2941 caattgcttc cggcagttcc gccccgtaat ccgctttcag cgtttcacac agccatttac
     3001 catgttgggc gatctgttct tttttaccga agacgcgcag ggtgttgaga tagagcagct
     3061 gcgcgtcttc gtcttccggc aacaaccgcg aaagctgctt taatgtttca agcccgcagg
     3121 tgttatcggg ctgtatccgg taagctcggg caatgatgcg aagagccgaa gtaagccttt
     3181 ggcgcagatt ttcctgaaga ccttcgctcc acaagtagcc gcagctggca aaaaggccct
     3241 tctgaaagat ttcacaggcg ctttcaaccg cattgtaagt ttcgggcatg ttgccctgaa
     3301 atcgggccag agcgtcttca gtatccaaat aatcgcattg gacgcccgat aagaacaggc
     3361 cgtatttatt atcggcatat atcagcttta cctcgtccct gaagtctgaa aaaatgttcc
     3421 ggattttgca gacggaggtc tggaggatcg gcagtgattt cgtgtattcg taatccggcc
     3481 acaggttttc aagaatgata tccttattta cgaaaatacc ttggtgaagc aacaggtaag
     3541 caaacagttc ctcagccttt aggcgcttcc atattaccgg ccgcccgttc ataacggcct
     3601 cgaatccgcc gaagcagcga atggtcagag cactttcctc ctctgtctca ttcgttttaa
     3661 attggctttt cagcctttca gccattttct gaaaccgggc tttgttaagc ggtttcatga
     3721 tgtaatcgag cgcgttgagc tcaaaggccg caactgcgta ctgattccaa cccgtgataa
     3781 agacgatttt catttccgga tacgcttcaa gcagtttctc ggcaagcgtc aggccgtcca
     3841 tttcaggcat ctcgatgtcg ataaaagctg cttggggtag gacgcgggcc gcttccctga
     3901 gtgcgtcggc accattctga cagcggctcc gtaccgtcat aaaaccggtc ttttcgacca
     3961 tgtcgcaaat ttcctcgaga ttataccact cgtcgtccac aacaatcgtc cgaatcattt
     4021 tcagccttcc cttacagctt ttgttagcgg aatgcggaac gatacggagg ttccggatcc
     4081 gcttttaata ttcaagccct ctccataata ttttaaaagc cttttatgaa tgttgatcat
     4141 gccgactccc tgtttgtcgt cctctccgga aagcagggac ggaattcttg ccggttcgat
     4201 tcccacgccg tcgtcgctga tttcaaagcg tgcgcattcg ccatccgttt caacagagag
     4261 acggacggtt ccaccgtctt ttttcggctg gaggccatgg cgaatggcgt tttcgacgag
     4321 cggctgcagt ataagtcgcg gaagttccag agccagaggc gcggtcacct ccgctgtgta
     4381 acggattcgt ccgccgaaac gtgcatatac gatttgtacg taagtatcga cagcctccag
     4441 ctcgtgttcc agcgtatcca taggagaaga actgccgatc cggtaacagc tgatcaggta
     4501 ttcgctgaga ttcacgacaa ggtcttttgc tgcatcgtcg tctctggaaa tcagggagca
     4561 aattgcgctc agcgtattgt tgatgaaatg ggggcttatc tgtgcttgca aaaacgacag
     4621 ttcgttagtt tccgctcttt cgttcgcctg ctcggcaagt tgttttagct gattctggta
     4681 ttttacaaaa agcgagatgt ccttgatttg cacaatgttc ccgatgggaa tcccgctttc
     4741 cgtctggatg ccgataagtt ctgcggagaa gtagcgtttg agctggttgt cttccttgta
     4801 aaaacggatt tttgattttt ccggggacaa cccgcgcagc tcgtggtggc gggccaggaa
     4861 atcgttttgt gtgcttttca ccgttagccg tttcatttcc ggatacagtt tcagaaaggc
     4921 tgcattcacg tctgttatgc gtccgctgtt gtcgataaga gcgatcgcat ccccggacag
     4981 cctgtaaaca gtctcggtcg caatcggaac cgtgttgaaa atatcgtagc ggaacagccc
     5041 gaacaggtat aggattccgg taccaatcat catcagggaa taatagtcaa tgcggagaaa
     5101 ggtagaattt ctgatattga aatatacggc aatccagggc agaaaagaag ccagaatcat
     5161 taggaggtaa ctcccgcggt acattcttac cgattcaagg tatccgcgga tg
//
LOCUS       DATALY010000303         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_66644,
            whole genome shotgun sequence.
ACCESSION   DATALY010000303 DATALY010000000
VERSION     DATALY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66644"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VHR42_08210"
     CDS             complement(<1..414)
                     /locus_tag="VHR42_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3027194.1"
                     /translation="MLKKIICSVLVFTLLAAFPFPAFADTQPQVTAKAAVLINADSGQ
                     IIYAKEENKRLSMASTTKIMTALITLEEAAVNNRIITITNEMVRVEGSSMGLLPGDQI
                     TLRSLAEGMLTVSGNDAANSAAIAIGGNLERFARKM"
     gene            complement(467..931)
                     /gene="ytfJ"
                     /locus_tag="VHR42_08215"
     CDS             complement(467..931)
                     /gene="ytfJ"
                     /locus_tag="VHR42_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HEX3027195.1"
                     /translation="MSDHPIEGMMNTTLEKIKQMVDINSIVGDPITSPDGSIIIPVSK
                     ISYGFASGGSDFPSKVQTEKEFFGGGTGAGVSINPVAFITICNGSVKLLQIDPYNSTA
                     DRIIGMFPEVVDKISDFVSSKKGENKKSRAEKKDDFKSSDRTGDVKLEDPQI"
     gene            complement(933..1592)
                     /locus_tag="VHR42_08220"
     CDS             complement(933..1592)
                     /locus_tag="VHR42_08220"
                     /inference="COORDINATES: protein motif:HMM:NF022615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HEX3027196.1"
                     /translation="MTALIIIASVLLLFLILLLCPVTIYAHYENEATVKIRYLFFRYT
                     VVPQPAEEKEEKQQPEKKEENIEKVDETESVIRDIIKQRGLSGFLNIVKRLASIATGT
                     AKKLLSHMVIDRISTDIAVADGDAAQTAILYGNVCSVVYTSFGVLVNNMRCKDYHINI
                     VPDFQSEESRIRFDGKVHILLLFIISYGLTAMIRFIKVLKETGILSKFKRTKMKNKAV
                     H"
     gene            complement(1623..2174)
                     /gene="scpB"
                     /locus_tag="VHR42_08225"
     CDS             complement(1623..2174)
                     /gene="scpB"
                     /locus_tag="VHR42_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX3027197.1"
                     /translation="MEIKKLQGAIEAILFSSGDPVSVNRIAEALELDVPTASKMLKNL
                     MDEFNRPDAGIRIVKLENNYQMCTNPEYAEHVRKVMDMRRNTPLSQAAMEVLAIVAYN
                     QPVTKAFIEQIRGVDCSGVLGSLTSKGLIEERGRLELPGRPLLYGTTPDFLRCLSISS
                     LSELPPLPQKQPSEEETAEASDA"
     gene            complement(2256..2477)
                     /locus_tag="VHR42_08230"
     CDS             complement(2256..2477)
                     /locus_tag="VHR42_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027198.1"
                     /translation="MAAYLNAAGRGRRFLPPPAEAFSGIVSHKVVSVASRIIFVLRRL
                     WRKNEVKYESLFEEKQEKIGTGCHFFSGT"
     gene            complement(2595..2885)
                     /locus_tag="VHR42_08235"
     CDS             complement(2595..2885)
                     /locus_tag="VHR42_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX3027199.1"
                     /translation="MEKLSYKLPSFEGPLDLLLYLIGKNKLNICDVQISELLEQYMEQ
                     IDVMKEQDMDIASEFLEMAARLVYIKTVYLLPKHEEAEELRRELTGQLLEYQ"
BASE COUNT          765 a          793 c          665 g          817 t
ORIGIN      
        1 cattttccgg gcaaatctct ccagattgcc gccgatggcg atcgccgccg aattggcggc
       61 atcattgccg gaaacagtaa gcattccttc cgcaagggat ctcagagtaa tctgatcgcc
      121 cgggagaagc cccatggagg aaccttctac ccgaaccatt tcattggtga tggtaataat
      181 tcggttattg accgccgctt cttccaatgt aatcagagca gtcatgattt tggtcgtgct
      241 ggccatggaa agccgcttgt tttcctcttt cgcatagatg atctgaccac tgtccgcatt
      301 gataagaacc gcggcctttg cggtaacctg cggctgtgtg tccgcaaaag ccgggaacgg
      361 aaaagcagcc aaaagagtga aaacaagcac ggaacaaatt atttttttta acaaagcaac
      421 cacctgccta taaaatatat gcaggcggct gcttcaatat tgactattaa atttgcggat
      481 cttccagctt tacgtctccg gtgcggtctg atgatttaaa atcgtctttc ttctccgctc
      541 tggacttttt gttttcgcct tttttgctgc tgacaaagtc gctgatttta tctacgactt
      601 ccgggaacat cccgataata cggtctgccg tgctgttgta cggatcaatc tgcagcagtt
      661 tgacgctgcc attgcagatc gtgataaaag caaccgggtt aatggaaacg cccgcgccgg
      721 tgccgccgcc gaaaaactct ttttctgtct gaaccttgga gggaaaatcc gaaccgccgg
      781 aagcaaagcc atagctgatc tttgaaaccg gaataataat cgatccgtcc ggcgacgtaa
      841 tcgggtcgcc gacgatggaa ttgatatcca ccatttgttt gattttttca agcgtcgtat
      901 tcatcatgcc ttcaatagga tggtcgctca ttttaatgca ccgccttatt cttcattttt
      961 gttcttttaa attttgacag gatgcctgtt tcctttaaga cctttataaa gcgaatcatc
     1021 gccgtcagcc cgtaggaaat aataaacagc aacaggatat gaacctttcc gtcaaagcgg
     1081 atcctacttt cctccgattg aaaatccgga acaatattga tatggtaatc tttacacctc
     1141 atattattga ccagaacgcc gaaggatgta taaaccaccg agcagacatt tccgtaaagg
     1201 atcgccgtct gggcggcgtc accgtccgca acagcgatat ccgtggaaat acggtcaatc
     1261 accatatggg acaaaagttt tttcgccgtt ccggttgcga ttgaggcaag cctttttaca
     1321 atattcagaa agcctgataa acctctttgt tttattatgt cccgaattac cgattctgtt
     1381 tcatcgactt tctcaatatt ttcttctttt ttctccggtt gctgcttttc ctctttctcc
     1441 tcagccggct gtgggacaac cgtatatcgg aaaaacaaat accgtatctt caccgtcgct
     1501 tcattttcgt aatgagcgta tatggtaacc gggcaaagca aaagaatcag aaaaagaaga
     1561 agaaccgacg cgataatgat caatgcagtc ataaagcctc caaaagcgat ttggaacgcc
     1621 gtttaagcat ccgacgcttc cgccgtttct tcttcactgg gctgcttttg cgggagcgga
     1681 ggcagttcac tcagcgaaga tatggaaagg catcttaaaa agtccggcgt ggttccgtaa
     1741 agcagcggcc tgcccggcag ctccagcctg ccgcgttcct caatcagccc cttcgacgtc
     1801 aagctgccga gcacgcccga acagtccacg ccgcgaatct gctcgataaa cgctttggtt
     1861 accggctgat tgtaggcaac gattgcgaga acctccatag ccgcctgcga aagcggcgtg
     1921 tttctgcgca tatccataac cttgcgcaca tgctcggcat attccgggtt ggtacacatc
     1981 tgataattat tttcaagctt tacgattcga atccccgcat ccggccggtt aaactcgtcc
     2041 atcaaatttt tgagcatttt tgaagcagtg ggaacatcca gttccagagc ttcggcaatc
     2101 cggtttacgg agaccgggtc cccgctggaa aaaaggattg cttctattgc tccctgaagc
     2161 tttttgattt ccaaaccttc gcaccgccgt tcatcaattt tattgttgca tcctcgctgc
     2221 cgccttcaat gcgaatcctt tttcccttta ccagttcaag taccgctaaa aaagtggcaa
     2281 ccagttccga ttttttcctg tttttcctca aacagcgatt catattttac ttcgtttttc
     2341 cgccacaggc gccgaagtac aaaaatgatg cgggaggcaa ccgacacaac cttatgggat
     2401 acgatcccgg aaaaggcctc tgccggcgga ggcagaaacc gtctgccgcg ccccgcagcg
     2461 ttcaggtaag ccgccaacag ctcctgcggg ctgtgcttcc gttcgtaagt attgtcaaat
     2521 tcaacctcag ccggttggcg cacaaagcag tcaaagccaa aacgttccgt taaaattccc
     2581 gctattttta tgcattactg gtattccagg agctggccgg tcagttcgcg ccggagctcc
     2641 tccgcctctt catgctttgg cagcaggtaa acggttttga tataaaccag gcgggccgcc
     2701 atctcaagga attcgctggc gatatccata tcctgttctt tcattacatc gatctgttcc
     2761 atatactgct ctagcagttc tgaaatctgg acatcgcaga tattcagttt atttttaccg
     2821 atcaggtaaa ggagcaaatc aagcggcccc tcaaacgacg gaagcttata ggatagtttt
     2881 tccatagtac cccgctttta taacaggccg aaaagatggt acagcgtttg ggcaagccaa
     2941 aacaccccat cgaaaaacag accctgcaga ttaccgatgg gtgcactcag cacaccgctg
     3001 aaaaggacta caaacgcaac cattacaatg atattctgat
//
LOCUS       DATALY010000304         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_582378,
            whole genome shotgun sequence.
ACCESSION   DATALY010000304 DATALY010000000
VERSION     DATALY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582378"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VHR42_08240"
     CDS             <1..1099
                     /locus_tag="VHR42_08240"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX3027200.1"
                     /translation="PDVFKSPVEKEKYYMTACTERARCILMESRFPEGPFSHPKLMTI
                     GGEPVKEIDPSVLVDDDGKVYIALPNFYVGQLDPSDYSKIIPETYHSVRDSMPLDNEP
                     FEGPSLKKRMGIYYYIYIQNKGKIETNGAVPVRMAYMTSKNPLGPYTYRGCILDTYDY
                     PGSGNVHGSFAQFNGNWYLSYHMAVPDLQLTRNACLDKLEFNLDGTIREVLPTSSGAK
                     GGFEVNETIQASGGVIFSGGRNDIRLRTRKEPTENPYVFHITGYPYTVYEKPEEWIGY
                     RYLNFSKRIKNVCVSVKTEAAGAVLEIRKGACDGKLIAEFYLPDTNGSWQTIKNKASS
                     TEDGTDTFFIIMKEKPGKGEVGVDHLIFTVD"
     gene            1377..1511
                     /gene="rpmH"
                     /locus_tag="VHR42_08245"
     CDS             1377..1511
                     /gene="rpmH"
                     /locus_tag="VHR42_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX3027201.1"
                     /translation="MLRTYQPKKIHRQKEHGFLKRMATRNGRKVLARRRTRGRHQLTY
                     "
     gene            1584..1922
                     /gene="rnpA"
                     /locus_tag="VHR42_08250"
     CDS             1584..1922
                     /gene="rnpA"
                     /locus_tag="VHR42_08250"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3027202.1"
                     /translation="MRITVPLKGNRDFRRIYSRAHSFVSRSVVLYFSKNRLGSNRLGI
                     TVSKKLGCAVKRNRAKRIIREAYRMTEPEIRRGYDFVVVARSRALSMKTGELQKDLYT
                     VLKNAGVLLK"
     gene            1919..2161
                     /gene="yidD"
                     /locus_tag="VHR42_08255"
     CDS             1919..2161
                     /gene="yidD"
                     /locus_tag="VHR42_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX3027203.1"
                     /translation="MKYLFMALIQGYKKFISPCLPPSCRFTPTCSEYAYEAFRRFGAI
                     KGFYLMMRRILKCQPFHRGGFDPVPETFEFFGRQSR"
     gene            2181..>3264
                     /locus_tag="VHR42_08260"
     CDS             2181..>3264
                     /locus_tag="VHR42_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX3027204.1"
                     /translation="MFDWIIRPFGYILYICYIGVRDYGLALLIFTLITRVLLFPLAIK
                     QQRSMAEMSRINPKLQQLQKKYAKDKQKLNEETMKLYQEEGYSPMSGCLPMLIQLPLL
                     TILYQVIMNPLKYILNFSQDNINALITALNLTAAQKSRAEIYISEKLSDPTQIANLVN
                     VHHLTFLQNMHPFNFYFLGIKSFVLTETPSWNHLNIYLLIPILCYITSFLSSWFNMKM
                     TQSMTPQQAQGMNKSMMITMPLVSTFFAFTVPSGLGLYWIYTNVFMIIQTLILNKFYH
                     PKKLAEEAERRAEEKRRLRAEAQQEQPLEEQVAEKTSSEETTNKPELQKRKPPQRPAV
                     HTKPNSKKQLKKQNAQRLASSRKSDGE"
BASE COUNT          950 a          736 c          807 g          771 t
ORIGIN      
        1 gcccgacgtg tttaaaagcc ctgtggaaaa ggaaaaatat tatatgactg cctgcacgga
       61 acgggcgagg tgcatcctta tggaaagcag gtttccggaa gggccgtttt cacacccgaa
      121 gctgatgaca attggagggg aaccggtaaa agagattgac ccgagcgttc tcgttgacga
      181 cgacgggaag gtctatattg cgctcccaaa tttttatgtc ggtcagcttg atccgtccga
      241 ttattctaaa attatccccg aaacctatca tagcgtcagg gactcaatgc cgttggataa
      301 cgagccgttt gaaggcccgt cgctgaagaa acggatgggt atctattatt atatttatat
      361 acagaacaaa ggtaaaattg aaacaaacgg tgccgtcccg gtaagaatgg cctatatgac
      421 gagcaaaaac ccccttgggc cttatactta ccgcgggtgt attttagata cctacgatta
      481 tccgggaagc gggaatgtac atggctcctt tgcacagttt aacggaaact ggtatctttc
      541 ttaccacatg gcggtgcccg atctgcagct cacgagaaac gcttgtcttg acaaactcga
      601 atttaacctg gacggcacga tccgtgaagt tttgccgaca tcgtccggag cgaagggcgg
      661 atttgaggtt aacgaaacga tccaggcatc gggcggggtg attttttcgg gaggaagaaa
      721 cgatatcagg cttcgcaccc gaaaggagcc gacagaaaat ccttacgtat ttcacattac
      781 aggttatcca tatacggtat acgaaaaacc agaggagtgg atcggctacc ggtatctgaa
      841 tttttcaaaa aggatcaaaa acgtatgtgt ttctgttaaa accgaagcag ccggcgcggt
      901 gcttgaaatt aggaagggcg catgtgacgg taaattgatc gcggaatttt atcttccaga
      961 cacaaacgga tcgtggcaga caatcaaaaa caaagcatca agtacagaag atggtacgga
     1021 tacgtttttt attataatga aagagaaacc cggaaagggc gaagtagggg tagatcatct
     1081 gatttttact gtagattaaa atattgtaat tgttttggtt taagccgggt aaaatgataa
     1141 ataaacgaac ggttttcgtc atgatgatat ggaataaaaa taatgaaacg taaaacggaa
     1201 gaaaaccggg cgcagggttg aaaaacaaaa acgggcggag ccagaaccgc gaaaatcggc
     1261 gaataggcac ggaatcagga caaagcccga ttttgattca aaaattgttg acaggacgaa
     1321 tggactcata tataatgata aaatgcaggg ttaggcccgg aagggaggtt ttttgaatgc
     1381 tgagaacgta tcagccgaaa aagattcaca gacaaaaaga gcatggtttt ctgaagagaa
     1441 tggccacacg gaacggccgc aaagtgcttg cgcgccgcag aacaagaggc cggcatcagc
     1501 ttacctatta aaacgccggg cacaagccgg cgtttttatg caacgggggt tgcggcgttt
     1561 taaaatatga atcgggggca gttatgcgca ttacagtccc gctgaaggga aaccgagatt
     1621 tcaggcgcat ctacagcagg gctcactcct ttgtcagccg aagcgtggtg ctgtattttt
     1681 ccaaaaacag gctgggcagc aacaggctcg gcattaccgt cagcaaaaaa ctgggctgtg
     1741 cggttaaacg aaaccgggcc aaacggatca tccgcgaggc atatcggatg acggagccgg
     1801 aaatccgccg cggctatgat tttgtagttg tggcgagaag cagggctctt tcgatgaaga
     1861 caggggagct tcagaaggat ctttacaccg ttttgaaaaa cgcgggtgtc cttttaaaat
     1921 gaagtacctt tttatggcgc tgattcaggg atataaaaag tttatttctc cctgcctgcc
     1981 tccaagctgc cgttttaccc ccacatgctc ggaatatgcc tatgaagcat tccggcgttt
     2041 cggcgccatc aaaggctttt accttatgat gcggagaatt ttgaaatgcc agccgtttca
     2101 ccgcggcggt tttgaccccg tgccggagac atttgaattt ttcgggcgac aatcgcggta
     2161 atgttggagg attttaaagt atgttcgatt ggatcatccg cccgttcgga tacatcctgt
     2221 atatttgtta tatcggcgtg cgtgattacg gtcttgcgct gttgattttt accctcatta
     2281 cgagggtgct gctttttcct ctggcaatca agcagcagcg ttccatggcc gaaatgtcac
     2341 gtatcaaccc gaagcttcag cagctgcaga aaaaatacgc gaaagataaa cagaagctga
     2401 acgaagaaac gatgaagctg tatcaggagg aaggctacag cccgatgagc ggctgcctgc
     2461 cgatgctcat ccagctgccg ctgctgacca tcctctatca ggtcatcatg aacccgctca
     2521 aatatattct aaacttttca caggataaca tcaacgcgct catcactgcg ctcaatctga
     2581 ctgcggcaca gaagtcaaga gccgaaattt atatttcgga aaagctttcg gatccaactc
     2641 agattgctaa tcttgtcaac gtccatcacc ttaccttttt acagaacatg catcccttta
     2701 acttctattt cctcggaatc aagtcatttg tcctgactga aacaccatca tggaaccatc
     2761 tcaacattta tctgctcatc cctatcctct gctacatcac gtcattcctc tcctcatggt
     2821 tcaatatgaa aatgacgcag tcgatgacac cgcagcaggc acagggcatg aataaatcca
     2881 tgatgattac catgccgctt gtttccacgt tttttgcttt caccgtgccg tcagggctcg
     2941 gtctctactg gatctatacg aatgtattca tgattataca gacgctgatc ctcaacaaat
     3001 tctatcatcc gaaaaagctt gcggaagaag cagagcggcg ggcagaggaa aaacgtcgtc
     3061 tgagagcaga agcacagcag gagcagccgc tcgaggagca ggttgcggaa aaaacctcgt
     3121 cggaggaaac aacgaacaaa ccggagcttc aaaaacgcaa acctccgcaa aggccggccg
     3181 tgcacacaaa gccgaattca aaaaagcagc ttaaaaagca aaacgcgcag cggctcgctt
     3241 catcgagaaa atcggatggc gaat
//
LOCUS       DATALY010000305         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_841250,
            whole genome shotgun sequence.
ACCESSION   DATALY010000305 DATALY010000000
VERSION     DATALY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841250"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VHR42_08265"
     CDS             complement(<1..879)
                     /locus_tag="VHR42_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027205.1"
                     /translation="MRGFLKTQKAMFRIMASTNANWLIYYLKKLPLVGKLLPESLYAN
                     IGLKRVLAVLATIVKAFRKFFGKVFFVGLLIALPAFLLEKTMPLRFDAFCHLLFVISL
                     FGPFMTSDIFLADRNRYICVRLMRMDARGLIVSAVLLDNAVDGLTLLPPVLLFTLLFG
                     GSFIQSLQLTVFSFVFGIVGEAFFLLFYAKTKVMLSKKAPFIMAAVLLCIMAAVLPFA
                     LHLPLAPKAVLLHPAATLLWAAVGALCIVYISRSGRYYGISAELLSASNVALNTDAAV
                     KNARIADVAVREKEFSA"
     gene            complement(919..1638)
                     /locus_tag="VHR42_08270"
     CDS             complement(919..1638)
                     /locus_tag="VHR42_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027206.1"
                     /translation="MQLELRNIQKSFEKKVVLRDIDFTFDQGKIYGLLGRNGAGKTTL
                     FNCLSGDLNPDSGEAYLLKDDRTQTLRPEDIGYVFSTPILPEFLTGYEFIKFYMEIND
                     IKRDALQPEQQTVDDYFNLVQIPPEDRHRLIKGYSHGMKNKLQMLCFIITRPPVILLD
                     EPLTSLDVVVALEIKKLLRQMRQEHVILFSTHILQLATDLCDEIVVLNEGRLEQIDHA
                     ILHDPEFEEKIISILKDESER"
     gene            complement(1696..>2040)
                     /locus_tag="VHR42_08275"
     CDS             complement(1696..>2040)
                     /locus_tag="VHR42_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3027207.1"
                     /translation="KSLMIAAFVGGRLAANCGINCAAPYKKYQHRAEFGIAVLEEFWG
                     NGIASALLTEIIAAARAMGYEQMELEVVTENTKAIALYKRFGFEIFGTRERSFKYLDG
                     SFASEHLMLLKL"
BASE COUNT          532 a          524 c          504 g          480 t
ORIGIN      
        1 agcggagaat tctttttctc tgacggcgac atccgcgatc ctggcgtttt ttacagcagc
       61 gtccgtattc agggccacgt ttgacgctga aagcagttcc gccgaaattc cgtaataccg
      121 ccccgaacgg gagatataaa cgatgcataa cgcgccgaca gccgcccaca gcagtgtcgc
      181 cgcaggatga agaagcacgg ctttcggagc gagcggcagg tgcagggcaa acggaagcac
      241 ggcggccata atacacagca ggacggcagc catgataaac ggtgcctttt tgctgagcat
      301 aactttggtc ttcgcataaa aaagcaggaa gaatgcttct ccgacaatgc cgaatacgaa
      361 ggagaaaacc gtaagctgca ggctctgaat gaagctgccc ccaaaaagaa gcgtaaagag
      421 aagaacgggc ggaagaagtg tcaggccgtc cactgcatta tccagcaaaa ccgcagaaac
      481 gatcaggccc ctggcatcca ttctcatcag gcgaacgcag atatatcggt tgcggtcagc
      541 cagaaagatg tcggaagtca taaacgggcc gaacaggctg atgacaaaaa gcagatgaca
      601 gaaagcgtca aaacgcagag gcatcgtttt ttcaagaagg aaggccggca gggcgatcag
      661 cagccccaca aagaaaactt tgccgaagaa cttccggaaa gctttcacga tggtcgccaa
      721 gacggccaga acacgcttta aaccgatatt ggcataaagg ctttccggca gaagtttccc
      781 gacgagcggg agctttttta aataataaat cagccagttg gcgttggtgg acgccataat
      841 ccggaacatc gctttctggg ttttcaaaaa tccgcgcatt ctgatataag ccctctcatt
      901 tccagtctta attcatgctt acctttcgct ctcgtccttg aggatactga taattttctc
      961 ttcgaattca ggatcgtgaa ggattgcatg atcaatctgt tcgagcctgc cctcgttgag
     1021 caccacgatc tcgtcgcaca agtcggttgc aagctgaaga atatgtgtag aaaatagtat
     1081 gacatgctcc tgccgcattt gccgcaggag ttttttgatt tccagcgcca caaccacatc
     1141 cagcgaagtg agcggctcgt caagcaggat caccggaggc cttgtgatga taaagcaaag
     1201 catctggagc ttattcttca tgccgtgcga ataacctttg atcagcctgt gccgatcctc
     1261 cggaggaatc tgaaccagat tgaaataatc gtccacggtc tgctgctcgg gctgtaacgc
     1321 atcccgcttg atatcattga tttccatata gaattttata aattcataac ccgtcaggaa
     1381 ctccggcaga atgggcgtgg aaaaaacgta tccgatgtct tccggtcgga gtgtttgggt
     1441 gcggtcgtct ttcaaaaggt aagcttcgcc gctatcggga ttgagatccc cgctcaggca
     1501 gttgaagagc gttgttttgc cggccccgtt gcgcccgaga agcccataaa ttttaccctg
     1561 atcaaaggta aagtcgatgt cgcgcaaaac cacttttttt tcaaaggact tttgaatatt
     1621 cctcagttcc agctgcatgc gtttttcccc tttttctttt ctggctgttg aacatttcgg
     1681 caaaaacggg ctgaattaca atttcagaag catcaggtgt tccgaagcga aggagccgtc
     1741 cagatactta aatgagcgtt cccgggttcc gaaaatttcg aagccgaacc ttttgtagag
     1801 cgcgatggcc ttggtgtttt cagttactac ctccagctcc atctgctcat agcccatcgc
     1861 tctcgcggcc gcaataattt ctgtaaggag agcggaggca atcccgttcc cccaaaactc
     1921 ctccagcaca gcgatgccga attccgcccg gtgctgatat tttttataag gcgcggcgca
     1981 gttgatcccg cagtttgcag ccagccttcc cccgacaaaa gccgcgatca tcaggctttt
//
LOCUS       DATALY010000306         6799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_327713,
            whole genome shotgun sequence.
ACCESSION   DATALY010000306 DATALY010000000
VERSION     DATALY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327713"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1858
                     /locus_tag="VHR42_08280"
     CDS             320..1858
                     /locus_tag="VHR42_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX3027208.1"
                     /translation="MRSENSMKNIVVNVSSQLFSIVIRFVCTTVFIQTLGVSYLGVNS
                     LFSNILTLFSLAELGVGSAITFSMYQPLARNDRKKIGALMNLYRKAYHTIGFIVAAAG
                     LAIAPFYRVFIKGDANIPNLTIIYFLYLFNSVITYFLSYKQTIITADQKNYVCVLYQN
                     AFCIVQNILQIFILYLTKNFILYLCIQILFSFLTNFSLSKKADKMYPYLKKYKDEKLN
                     SSDRKKIIHNIQAMFMHKIGGVIVNGTDAILISSFAGLVSAGIYYFYNLIILTLNGVI
                     QQIFNGITASIGNLGASEEDTRRPYHIYLSINFAGFWIFSFSAISLFCLFTPFIRLWT
                     GKNQVFTVLTEFFIALNFYATGMRQATLQFKNAYGLFWYDRYKAIAEAVINLVVSIIL
                     VQYIGIAGVFLGTFISTVTTDLWVEPLVLFKHGFHRSAAPYFGRYLFYTVLTFAVGFL
                     TWYSCYFVGGYSLRSFVLKCVICGILPNLIYYIIFCKTKEFQYLKGFFKIPKMKIKKK
                     IGVN"
     gene            1935..2837
                     /locus_tag="VHR42_08285"
     CDS             1935..2837
                     /locus_tag="VHR42_08285"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX3027209.1"
                     /translation="MVDVCLPGTGGMIPLENRWLTCCWVEYQGSAILIDCGEGTQIAL
                     KSAGCKLSHLDTLLITHFHADHIAGLPGLLLSLGSTGKTIPLTIVGPKGLNQVVSALT
                     IIAPSLPYPLHLYEIKENKEGSFEKDGTVVSFLPLSHWIPCFGYSITVRRKPIFNPEK
                     ANALGVPVTLFKRLHEGNRVQLENGRTVRPEEVLDGERDPIRVCYCTDTQPLDSIADF
                     AKNADLLISEGMYGDDTMRDKTQEKGHMLFSDSAEIARKAGAKRLWLTHFSPALTNPD
                     LYAGAAQKIFPSAAVAHDGMRITL"
     gene            2852..3709
                     /locus_tag="VHR42_08290"
     CDS             2852..3709
                     /locus_tag="VHR42_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX3027210.1"
                     /translation="MNILILGASGLIGSAVCRDSGADYRVFGTYLRNKCDIPVHWLKF
                     DISGDDIAKSLGTANPDVIIAGLRGDFNDQLRVHRQAADWAMQNKARLIFLSTANVFD
                     ASPDRPHTECDLPKAISEYGKYKIECERMLTGTLKEQAVIVRLPLVLNRKRLQTFVSN
                     NLSGNDRMYANFYRSINTDTAVAKAIRYIIENNLSGTVHLGSSDCIRDSDFMIKAAKR
                     LNLPADRLNEDKLTEESFCSALSCSSTDELTVQRNGSFYQVLNTNRADIPTEFHPSCE
                     EVINSAYAV"
     gene            complement(3761..5675)
                     /gene="cooS"
                     /locus_tag="VHR42_08295"
                     /pseudo
     CDS             complement(3761..5675)
                     /gene="cooS"
                     /locus_tag="VHR42_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
     gene            5785..6351
                     /locus_tag="VHR42_08300"
     CDS             5785..6351
                     /locus_tag="VHR42_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3027211.1"
                     /translation="MKFCVLMGSPRLNGNTAELAKPFIEELQNGGAEVDYITLADKKI
                     SPCKGCYVCQHVLTEYGCSQPDDMQQIVSAVLNSDCFVLAAPIYTWYCPAEMKAMLDR
                     FYGMNKYYGGYGSASLWKGKSCAIPATHGYDAEYGAKPFETGIRHLCEHSRLNYRGMY
                     SVRDLDDKASFQTEEAIRGAKEFARQIL"
     gene            6409..>6799
                     /locus_tag="VHR42_08305"
     CDS             6409..>6799
                     /locus_tag="VHR42_08305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX3027212.1"
                     /translation="MKNSALLIVDVQKALIAEHPYHERELLRNIRRLIDLCRENDTEV
                     IYVRHDSGEDDELGYGSDGWQIADEITPAEGEKTFDKKYNSAFKETGLKEYLDSKQIT
                     NLILVGMQTEYCIDTSCKIAFEYGYHLI"
BASE COUNT         1726 a         1639 c         1624 g         1810 t
ORIGIN      
        1 agcatcgagg gagtctgcgg aaagaatgga tttatgttca atattcattc cccaaaagac
       61 cctgaaaccg gagacggcgg tcagtcggct gtcgcccggc ttcaggcggt aaaaatcacg
      121 gattaattca gcatactgtt gctgtgtgta tcaaagcaag acccggccag gcagattttc
      181 caaaagaggg agtaacaaaa tgagtataaa ctcattttgt tactccctct ttttagtgat
      241 ctataattta tactgtgttc ataaagtaat actataatat ctgaaacatt attgtaagca
      301 ttctgaaagg tgtggggttg tgcgcagcga aaactcaatg aagaatattg tcgtaaacgt
      361 atccagccag cttttctcca ttgtgatacg ttttgtctgt acgaccgtgt ttattcagac
      421 gctcggcgta tcctatttag gcgtaaactc cctgttcagc aacatcctga cgctgttttc
      481 gcttgcggaa ctcggagttg gttctgcaat tactttcagc atgtatcagc ctcttgcacg
      541 caacgaccgt aaaaaaatcg gcgcgctgat gaacctttac agaaaagcat accatacaat
      601 cggatttatc gtggctgcgg ccgggcttgc catcgcccct ttttacagag ttttcattaa
      661 aggtgacgcg aacattccca acctgacgat aatctatttt ctttatctat ttaattctgt
      721 cattacctat tttttatcgt ataagcaaac tattattacc gccgatcaga aaaactatgt
      781 ttgtgtgctt tatcaaaatg cgttttgtat tgttcagaac attctgcaaa ttttcattct
      841 gtacttaaca aaaaacttta tcctctatct atgcatacaa attcttttca gctttttaac
      901 aaacttttcc ctgtcaaaaa aagcggataa aatgtacccg tatttaaaaa aatataagga
      961 cgaaaagctg aacagttcgg atcgaaaaaa gataatccat aatattcagg ccatgttcat
     1021 gcataaaatc ggcggagtta ttgtgaacgg aaccgacgcc atcctgattt cttcctttgc
     1081 cggcctggtc agcgcgggaa tttactattt ttataatctg atcatcctca cgctgaacgg
     1141 cgtaattcag caaattttca acgggataac cgcaagtatc ggtaacctcg gggcttcgga
     1201 ggaggacacc cgaaggccct atcatattta cctgtcgatt aacttcgccg gattctggat
     1261 tttcagtttc agcgcgattt cccttttctg tctgtttacg ccgtttatcc ggttatggac
     1321 gggaaaaaat caggttttta ccgttttgac cgagtttttt attgcgctga atttttacgc
     1381 caccggaatg cgtcaggcaa ccctgcagtt taaaaacgca tacggtctgt tttggtatga
     1441 ccgctataaa gcaattgcgg aagctgtgat taacctggtt gtttcgatta ttctggtgca
     1501 gtatatcggc atagcgggtg tctttcttgg aactttcatc agcaccgtaa caaccgacct
     1561 gtgggtagag cctcttgtgc tgttcaagca cggatttcac cgatccgctg ccccgtattt
     1621 cggccgttac ctgttttaca ccgtactcac attcgccgtc ggttttctca cctggtactc
     1681 ctgttatttt gtcggggggt actccctgcg aagctttgtc ctgaaatgtg tgatctgcgg
     1741 aattcttcct aacttaatct attatataat tttctgcaaa acgaaggaat ttcagtatct
     1801 aaagggattt tttaagattc caaaaatgaa aatcaaaaag aaaatcggag taaattaacg
     1861 gtaagcaggc atttgttcag cgtacccaaa atctttatcc gataattcac ttggttggaa
     1921 atggggcgat gaatatggtt gacgtatgtt tgccgggaac gggcggcatg attccgctgg
     1981 aaaaccggtg gctcacctgc tgctgggttg aatatcaggg gagcgcaatt ctgatcgact
     2041 gcggtgaggg gacccaaatc gcgctgaaaa gcgccggctg caaactcagc cacctcgaca
     2101 ctctgttaat tacccacttt catgccgatc atattgcagg cctgccgggt ctgctcctat
     2161 cactgggcag caccggaaaa acgataccct taacaattgt cggcccgaaa ggactgaatc
     2221 aggttgtttc cgcgctgacc attatagcgc cttccctgcc ctaccctctg catctttatg
     2281 aaattaagga aaataaagaa ggctccttcg aaaaggacgg aacagtggtc tcgtttctgc
     2341 cgctcagtca ctggattccc tgcttcggct acagcataac cgtaaggcga aagccaatct
     2401 tcaatcccga aaaggcgaat gctcttgggg ttccggtcac tttattcaag cgtctgcatg
     2461 aggggaatcg cgtgcagctg gaaaacggac gcaccgtcag gccggaagag gttctggacg
     2521 gagaacgcga cccaatacgg gtctgctact gcacggatac acagccgctt gactctatag
     2581 ccgatttcgc gaaaaacgcc gatttgctga tcagcgaggg tatgtacggc gacgacacaa
     2641 tgagggacaa gacgcaagaa aagggacaca tgctgttttc cgacagtgcc gaaatcgcga
     2701 ggaaagccgg tgcgaagcgg ctgtggctaa cacatttcag tcccgcgctc actaaccctg
     2761 acctatacgc cggggctgca cagaaaatat ttccgtccgc cgccgtggca cacgacggaa
     2821 tgcggattac tttataatca gggtgtttat tatgaatatt ttaatcttag gcgcaagcgg
     2881 cctgatcggc agcgcggtct gccgcgattc aggagcggat taccgggttt tcggcactta
     2941 tctaaggaac aaatgcgata ttcctgttca ttggctgaaa tttgatattt ccggtgatga
     3001 cattgctaaa tctttaggaa ccgcaaatcc ggacgttatt atcgccggac tccgagggga
     3061 ttttaatgac cagcttcggg ttcacaggca ggcggccgac tgggcaatgc agaacaaagc
     3121 gaggctgatt tttctttcca ctgcgaacgt attcgacgcc tctccggaca ggccgcacac
     3181 ggaatgtgac cttccaaaag caatcagcga gtatggaaaa tataagatcg agtgcgagag
     3241 gatgcttacc ggaacattaa aggaacaagc cgtcattgtg cgtctgccct tggttttgaa
     3301 tcgaaaacga ctgcaaacat ttgtttcaaa taacctttcg ggcaacgaca gaatgtatgc
     3361 gaacttttac cggagcatta ataccgatac tgctgtcgca aaggcaatcc gttacataat
     3421 cgagaacaac ctttccggaa cggttcattt gggaagcagc gactgtatca gagactcgga
     3481 ctttatgata aaagcggcga aaagattaaa cctgcctgcc gataggctga atgaagataa
     3541 gcttacagaa gaatctttct gctcggcgct tagctgcagt tcaaccgacg aactgaccgt
     3601 gcagcgaaac ggaagctttt atcaggtttt gaacacgaac cgcgccgata ttccgacgga
     3661 gtttcatcct tcctgcgagg aagtaattaa ttcagcatac gctgtctgaa ataaaaaaac
     3721 ggcggacaga taaactgtcc gccgacggat taaatcgaaa ttaagccagc cccaggcctt
     3781 tacgcttgtc catgatatgc ttttcaaggt cgtttgcaac ctccaccggg tcatcgccga
     3841 cggttacttt ccctccggtc agcgtttcga catcctgcgt cagcagcttc acaaggttcg
     3901 gagcgcccgt gataaacggg gtcggcgaca ggtgtgtgta agcgccgtaa gcaactgcga
     3961 aaatgccgtc gatggtcgcc ttttgctcca tccattccgg cgcggtaact gcaatcggca
     4021 gctgcggcac atcgacatcc agaaaatcgg ccagcgcggt cacgagcatg gagattctgc
     4081 cggtatcggt acaggttcca aagctcagca ccggaggaat tttcagcata ttgcagactt
     4141 cctttaaacc cgctccggcc atttccgccg cacttgtgtc gcaaaggcca gcaacttcca
     4201 aggcatggtt gccgcagccg cagcttacaa caagaatatc ctttttaatg agttcctttg
     4261 tgagattcac ggtattccag tcttgcggac cgttccgaag cgtggagcag cttgcaaggg
     4321 ccacaacacc ttttattttg cccgccttaa ttacgtccac aagcgggtcg agtttgttgc
     4381 ccaaagcgcc gagtaccgct tcggtcgaaa agcccgcaat ggctttctgc gttttttttc
     4441 ggcaccatcg gggtaatttt accatgtctc ttcttaaagt tttcaatgcc cagttcaatc
     4501 aggcggtctg ccattttttc ggcttcgcac ggatggtacg gcagctgttc gctcagaccc
     4561 ggcacgccga ttatcgtgct gatgctcacc agtgttgcct gatatttttc cgcatactgg
     4621 tctatcgccg gcggggagca gttttcgtcc attgccagaa catcgaccgt accggtggca
     4681 agcagcggtt caattgcaag ccagtcgccc atcaggccga caaaggtttc gtcaaccgga
     4741 aagcgctgca aaagctcctg accggtttca atggagccga cgatgcgcag tcctttcgca
     4801 ccggcttcct ttgccctttg ctgaacttgt tcggtcttcg ctttctgcag tgtggcaaca
     4861 ccgacccacg gctgatgtcc gttaaacacg atgttgatat agtctccgtc cagaataccg
     4921 aggtcggtgt tgacctcatg cggggtagga gtgccgtaca aaatgtcctg caccatttcc
     4981 agcccaatct gtgcggaata aatggttgca atgccaaggc gcagcgcctt ttttgctaac
     5041 gatacataat cgccatctac atttgtcagg cagcttgcaa cgcagttctg ttcctcgtga
     5101 ataacgccgg ccgggtaaat attcaggttt ttccagacct ctttgcgttt tttcggcgca
     5161 aaggcttcga ccataatgtt cggctcgcct acacccttgg acatttcggc gtccagcaga
     5221 tttgcaagct gctcggccat ctgattttca ctttgttcgg tgttaatgcc taccttctgg
     5281 cacatccatt tcagcttgtt gatatcggtc agcttaaagc aggtcttccc ctgcgccgtt
     5341 tcccgcagag tacggaacgc ctcgtaggca tggtggctgt aagtaccggc gcccataata
     5401 ttgtgcagca aaagggtacg cattgccatg gcgtccgggc cgattccgca gacgcccttg
     5461 tcttgaccgc ctttttcgct gatgcggcaa ggcccgtccg agcaaagctg gcagcttagt
     5521 ccttccagac agaatttaca ccgtattttt tcctgcagcg cgtaccggtc aaacgcattc
     5581 gaaaggccgt cttcgcggat tcgtttaagc atttcctcaa ccgaatcatg gtagcttacc
     5641 cgaccttccg ttttctccaa aatagtctct tgcataaata acctcctctt agttaataag
     5701 gctattcttt ccgattcttc cgcagttagt atatggagtt ttattttctg taaatttaac
     5761 agatgttgat tcggaggtgt taatttgaag ttttgtgttt taatgggaag cccgcgttta
     5821 aacggaaata ctgctgaact cgcaaagcct tttatcgagg aactgcaaaa cggcggcgcg
     5881 gaggttgatt acatcacact tgccgataag aagatttcac cctgcaaagg gtgttacgtc
     5941 tgccagcatg tcctgactga atacgggtgc agtcagcctg acgacatgca gcagattgtt
     6001 tccgctgtcc tgaacagcga ctgttttgtt ttggctgctc ctatttacac atggtactgc
     6061 cctgccgaaa tgaaggcaat gctcgaccgc ttttacggca tgaacaaata ttatgggggc
     6121 tatggctcag cttccctgtg gaaaggaaaa agctgcgcaa ttccggcgac tcacgggtat
     6181 gacgcggaat acggcgcgaa gccgttcgaa accggaatcc ggcatctctg cgaacattca
     6241 aggttaaatt accggggcat gtattcggta agggatttgg atgataaagc ttcttttcag
     6301 acggaggaag cgattcgggg tgcgaaggag tttgcgcgac aaatattgta aaataatata
     6361 gatacaattg gagatcagtg ttaaaatagg acaaacggag ggataaaaat gaagaattca
     6421 gccttactta ttgttgatgt gcagaaagca cttatagcag agcacccgta tcacgaaagg
     6481 gagctgcttc ggaatatcag gcggctgatt gacctgtgca gggaaaacga tacggaggtg
     6541 atttatgttc gtcacgacag cggcgaggac gacgaattgg gttacggctc cgacgggtgg
     6601 cagattgccg atgaaatcac ccccgccgaa ggtgaaaaga catttgataa gaaatataac
     6661 agcgctttta aggaaaccgg gctaaaagag taccttgaca gcaaacagat tacaaacctg
     6721 attttggtcg gtatgcagac agaatactgc attgacacaa gctgcaagat cgcgtttgaa
     6781 tatggttatc atcttatca
//
LOCUS       DATALY010000307         4776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_411192,
            whole genome shotgun sequence.
ACCESSION   DATALY010000307 DATALY010000000
VERSION     DATALY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411192"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /gene="der"
                     /locus_tag="VHR42_08310"
     CDS             complement(<1..1123)
                     /gene="der"
                     /locus_tag="VHR42_08310"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX3027213.1"
                     /translation="MSKKIVAIVGRPNVGKSTLFNKLVGRRISIVEDTPGVTRDRIYA
                     ECEWSGRSFMLVDTGGIEPKTDDILLKQMRSQAQMAILTSDVIILVTDLRAGVTASDA
                     DVAQMLLKSGKPIVLCVNKADTVGGVPEEIYDFYNLGLGDPVAVSSVHGHGTGDLLDR
                     VLEELGPEEQEEESDDTIRVAVIGKPNVGKSSLINKIAGEDRVLVSNIPGTTRDAVDT
                     DIVNEYGKFTFIDTAGIRRKSRVDDNIERYSVLRAYMAVERADVCVIMIDANEGFTEQ
                     DSKIAGYAHEKGKASIIAVNKWDVVEKDEKTMDGYRKKLMNDFSFMSYSPIIFISAKT
                     GQRISRLFELIQFVHNQHAMRISTGMLNDVLSDATARVQP"
     gene            complement(1157..2461)
                     /locus_tag="VHR42_08315"
     CDS             complement(1157..2461)
                     /locus_tag="VHR42_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX3027214.1"
                     /translation="MSVLVTGVEPGSPAEKENIRAGDTIFSINGHEILDVLDYRFYMV
                     ETGLKIILGDSKGKKRKIFLEKEEYEDPGLDFESYLMDQKRSCSNGCIFCFIDQLPRG
                     MRESLYFKDDDARLSFLMGNYITLTNLTQREIDRIIHMHISPVNVSVHTTNPELRVRM
                     MKNRNAGKCLEIIQKFASAGIKMNCQLVLCKGINDGVELDRSLRDLCSLYPAVESVAV
                     VPVGLTRYREGLTALEPYSREGAREVVEQAERFGSRFLKEHGARIVYAADEFYIKAGQ
                     EIPPAAFYGEFNQLENGIGLIALLQSQFDDALDKMALKKPRSISIATGKAAHSFLEKL
                     SSRAQEAVAGLNCTVYAIENEFFGKNVDVAGLVTGKDLIRQLKGKRLGEELLIPAVML
                     RSERDVFLDDLTVADVEKALAVRVLPVENDGYELFDAMTGRK"
     gene            complement(2577..2759)
                     /gene="rpmF"
                     /locus_tag="VHR42_08320"
     CDS             complement(2577..2759)
                     /gene="rpmF"
                     /locus_tag="VHR42_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX3027215.1"
                     /translation="MAVPKRKVSKARRDKRRSNVWKLEAPGLVKCPQCGELKMPHRVC
                     GSCGYYNGKEVVKKEA"
     gene            complement(2829..3323)
                     /locus_tag="VHR42_08325"
     CDS             complement(2829..3323)
                     /locus_tag="VHR42_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX3027216.1"
                     /translation="MLLELRRIFAGEGPELEINYTIDLSSLEIAPGKYPFKEPVSVRG
                     RVRNKADVVELRAEAVFDFFTHCDRCCREITEHLTVPLNYVLATSAQSEDSDDIIVVE
                     NEKLDLDELAVSNIILSLPMKHLCKEDCKGLCPVCGKNLNDGPCGCGGEIDPRLAALK
                     DLFE"
     gene            complement(3552..3962)
                     /locus_tag="VHR42_08330"
     CDS             complement(3552..3962)
                     /locus_tag="VHR42_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027217.1"
                     /translation="MLQLIVGKKGTGKTKQLISMVNDAVGVSKGKVACIEKGTKLTYD
                     INHDVRLLNTDSFQITGYDAFYGFLAGIVASDYDVKEIYVDSLTKIVGSDLNAIETFL
                     EEVNLLSSNEEISFTMTISVDAGELPEKVKKFII"
     gene            complement(4118..>4776)
                     /locus_tag="VHR42_08335"
     CDS             complement(4118..>4776)
                     /locus_tag="VHR42_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX3027218.1"
                     /translation="ADKARKELANPEIGSNDCYKYAAARNILSRKGKSIEMLVSYEPS
                     FALMNEWWKQLFGESEGKDNKGIFPAAAVYSTDLHSMGQFVQEGSREMFETVVHIAKP
                     KSDYVIDTIDGDIDGLNFLSGKTMQFVNDRAFEGTVLAHTDGGVPNIVLEIPEINETE
                     FGYLVYFFEKACAISGYILGVNPFDQPGVESYKKNMFALLGKPGYEKEKASLEARLGK
                     "
BASE COUNT         1173 a         1301 c         1014 g         1288 t
ORIGIN      
        1 gaggctggac tcgggcagtc gcatcggaga gcacgtcatt gagcattccc gttgaaattc
       61 tcatcgcatg ctgattgtgg acaaactgaa tcagctcaaa caatcggctg atccgctggc
      121 ctgttttagc ggaaataaaa ataatcgggc tgtaggacat aaaactgaaa tcgttcatca
      181 gctttttgcg gtatccatcc atcgtttttt catctttttc aacgacgtcc catttattga
      241 ccgcaataat actggctttg cccttctcgt gcgcatatcc ggctatttta gaatcctgtt
      301 cggtaaaacc ttcgtttgca tctatcatta taacacatac atcggcacgt tcaacagcca
      361 tatacgcccg gagcacacta tatctttcaa tattgtcgtc aactctgctt tttctccgta
      421 tgccggccgt atcaataaac gtaaatttgc cgtattcatt gacgatgtcg gtatcaacgg
      481 cgtctctggt cgtgccggga atgtttgaaa ccaggaccct gtcctccccg gcaattttgt
      541 taatcagtga agattttccg acattcggct tgccgatgac ggcaacacgg attgtatcgt
      601 cggactcctc ctcctgctct tccgggccaa gctcctcaag cacgcggtca agcagatcgc
      661 ccgtcccatg tccgtgcacc gaggacaccg cgaccggatc gcccaggccg agattgtaga
      721 agtcataaat ttcttcgggc acaccgccca ccgtgtcggc cttgttgacg cagagcacaa
      781 tcggcttacc gcttttaagc agcatttggg cgacatcggc atccgacgcg gtaacaccgg
      841 cgcgcagatc ggtcaccaaa atgatcacat cgctcgtcaa gattgccatt tgagcctggg
      901 aacgcatttg cttgagcagg atatcgtcgg ttttcggctc gattccgccc gtatccacca
      961 acataaagga acgcccgctc cattcgcatt cggcataaat ccgatcacgc gtcacaccgg
     1021 gtgtatcctc aacgattgaa atacgccgtc ccacgagttt attaaaaagc gtggattttc
     1081 ccacattcgg ccttccgaca atcgccacga ttttttttga catagtgtcc tccatctttt
     1141 ctgaaacgcg accggtttat tttcgcccgg tcattgcgtc gaacagttca tagccgtcat
     1201 tctccacggg gagaacccga accgcaagcg ctttttcaac atctgccaca gtcaggtcgt
     1261 ccaggaaaac atcccgctcg ctcctaagca ttacggcggg aatcaacaat tcctcgccca
     1321 ggcgtttccc tttcagctgc cgaatcagat cttttccggt tacaagaccg gccacatcta
     1381 cattttttcc aaaaaactcg ttttctatcg catataccgt gcaattaagc cccgcaaccg
     1441 cttcctgagc ccgagaggaa agcttttcca gaaacgaatg cgccgccttt cccgtcgcga
     1501 tcgaaatgct ccggggcttt ttgagggcca ttttatcaag cgcatcatcg aattgacttt
     1561 gaagcagggc aatcagccca ataccgtttt ccagctggtt gaattcccca taaaaagcgg
     1621 caggtggaat ttcttgtccg gctttaatgt agaattcatc cgctgcgtag acgatacggg
     1681 cgccatgctc tttcaggaaa cggcttccga aacgctccgc ctgttcaacg acttcgcgcg
     1741 cgccttcacg ggaatagggc tccaatgccg ttagtccttc gcgatagcgg gtcagaccaa
     1801 cgggaacaac agcgacgctc tccaccgcag gataaagcga gcacaagtcc cggaggctcc
     1861 tatcaagctc gacaccgtcg ttgatccctt tgcaaagcac aagctgacaa ttcatcttta
     1921 ttccggcaga tgcaaatttt tgaatgattt caaggcattt tcccgcgttt ctgtttttca
     1981 tcatccttac ccgaagctcg gggtttgtgg tgtgaacgga aacattcacc gggctgatat
     2041 gcatatggat aattctgtct atctcccttt gcgtaaggtt ggtgagcgtg atgtagttgc
     2101 ccattaaaaa ggacagcctc gcgtcatcat ccttaaaata aaggctttcc ctcataccgc
     2161 gcggaagctg atcaataaaa cagaaaatgc agccgttgga gcagctgcgt ttctgatcca
     2221 tcaggtaaga ctcaaaatcc aacccgggat cttcatattc ctctttttca agaaaaatct
     2281 tgcgtttttt tcccttgctg tcgcccagaa taatcttcag cccggtttcc accatataga
     2341 accgataatc aagaacatca aggatttcat gaccattgat ggaaaagatg gtatccccgg
     2401 cccgaatatt ttctttttcc gccggactcc ccggttcaac ccccgtaaca agtacgctca
     2461 ttttcaatct ccccatcaaa aaaaccgatg gcaaaaaagc ctccgaccta ttctggaagg
     2521 cttcctttca tatgcaaaaa aatagagaag gatttctcct tccccatctt tactcatcag
     2581 gcttctttct tgacaacttc cttgccgttg tagtagccac agctgccgca aaccctgtgg
     2641 ggcatcttca gttcgccgca ctgcgggcat tttacaaggc cgggcgcttc aagtttccaa
     2701 acattcgagc gtctcttatc acgtctcgct tttgaaactt ttctcttcgg aaccgccatt
     2761 ttaagtacct ccctttaatg tggaaatcat tccacaatcg cttatcattg gtaacctgta
     2821 actcgctgtt attcgaacag atccttcagc gccgcaagtc tgggatcaat ttccccaccg
     2881 catccgcacg gaccgtcgtt gaggtttttt ccgcaaacag ggcagagccc tttgcaatcc
     2941 tctttgcata aatgcttcat cggcaggctt aaaatgatat tggaaacggc aagctcatca
     3001 agatcgagct tttcattttc aacaacgata atatcatcgc tgtcttcact ttgcgcggag
     3061 gttgccaaaa cataatttaa cggaaccgtc aaatgctcgg taatctcccg gcagcacctg
     3121 tcacaatgcg taaaaaaatc aaagaccgcc tcagccctta attctaccac atcggccttg
     3181 tttctgaccc ggcccctgac ggagaccggc tctttaaatg gatatttgcc gggcgctatt
     3241 tcaagcgaac tcaaatcaat tgtgtaatta atttcaagtt ccggcccttc gccggcaaaa
     3301 atccgcctta actcaagaag catcgctaca cctcagaatg tcactcaaac attataaatt
     3361 tgaaaaagtc ttttgtcaag actttttatc ttatgccgcc cggttttacc tttgaaataa
     3421 tccgcgggca ggtgtttcca gaagaaaacg gcggccgaat tcgcggaaga ctgccgcaga
     3481 tacggccgcc gaaaaggatc tttaaaattc gtttcgactg ttattatacc cgactttggc
     3541 caaaaaagaa tttagataat gaatttttta actttttcag gaagttcccc ggcatccacc
     3601 gagatagtca tcgtgaaaga gatttcctca ttcgaggaaa gcaggttgac ttcttccaaa
     3661 aaggtttcga tcgcattcag gtcgctgccg acaatcttgg taagagaatc cacataaatt
     3721 tctttcacat cataatccga cgccacgatt ccagcgagga aaccgtaaaa cgcgtcgtaa
     3781 ccggtaatct ggaagctgtc ggtgttgagg agtctcacat cgtggttgat gtcataggta
     3841 agcttggtgc ctttctcgat acaggcgacc tttcctttgg aaacgccaac cgcatcattg
     3901 accatcgaga tcagctgctt tgtcttcccc gtgccttttt tgccgacgat taactgtaac
     3961 atgtgcatac ctcctgatat tttttattct tgaaatacca attaatcgga actcgctcgt
     4021 tgccgccgcg ccattgagac aggacggatc aacgcggtat ttccgtattt ggtattgcac
     4081 tggtttctca aattgatctg tggccaaaac cccgctgtta ttttcccagg cgggcttcca
     4141 gagacgcctt ttccttctca taacccggtt ttcccaaaag ggcgaacata ttcttcttgt
     4201 agctttccac acccggctgg tcgaacgggt taacgccaag gatataaccg gatatggcgc
     4261 aggctttttc aaagaaataa acaagataac cgaattcggt ctcgttgatt tccggaatct
     4321 ccagaacaat attgggtacg ccgccgtcgg tatgggcgag aaccgttccc tcgaaagccc
     4381 tgtcgttgac aaattgcatg gttttgccgg ataggaaatt caggccgtcg atatccccgt
     4441 cgatcgtatc aatcacataa tcggacttcg gttttgcgat atgaacaacc gtttcaaaca
     4501 tttcccgcga gccttcctga acaaactggc ccatggaatg aaggtcggtg gaataaaccg
     4561 ccgccgcagg gaagattccc ttattgtctt tgccttcgct ttcgccgaac agctgcttcc
     4621 accattcgtt catcagagca aacgaaggct catagctgac cagcatctcg atacttttgc
     4681 ctttgcgtga aaggatattg cgcgcagccg cgtacttgta gcagtcgttc gaaccgattt
     4741 caggatttgc gagctctttg cgtgccttgt cggctc
//
LOCUS       DATALY010000308         4181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_754564,
            whole genome shotgun sequence.
ACCESSION   DATALY010000308 DATALY010000000
VERSION     DATALY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754564"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1337)
                     /locus_tag="VHR42_08340"
     CDS             complement(282..1337)
                     /locus_tag="VHR42_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3027219.1"
                     /translation="MKNTFKKMISAVMALAMAASVTACSSGTASSASSAASTAASASA
                     GQAAKSFKIGVIQYAPHPSLDNCFKGFKAGLEEAGLKEGTNLTIDFQNAQGDVSNSDL
                     IAKNMVSKKYDLIMGIATPSAMSAFSAARTANIPVVFTAVSDPVAAGIVKSNDKPGNN
                     CTGSSDVLPLEQQVKMIRAFLPNAKKIGILYTTSEPNSVSQLKQIKEIAPKYNFEIVD
                     VGVTNASEVAAGAQTLVSKGVDCINNFTDNNVVNNLSTLLQAANSKKIPVFGSEEEQV
                     KNGCLASQSIDYVALGKETGKMAAKILKGEAKAQDMPVYVVKDYAPVYNKTVCQTLGL
                     TLPADYKNAAAVTTAAK"
     gene            complement(1779..2132)
                     /locus_tag="VHR42_08345"
     CDS             complement(1779..2132)
                     /locus_tag="VHR42_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX3027220.1"
                     /translation="MTKRELIEYCLTYPDAYEDYPFDEDSASSEAWTVMRHSGNRKSF
                     ALIYERGGKLRINLKCEPMLADFLRSVYPGVTPAYHMNKVHWNTVTPDEAIPEEELHE
                     WIRQSYDLTKPKKSS"
     gene            complement(2147..3400)
                     /locus_tag="VHR42_08350"
     CDS             complement(2147..3400)
                     /locus_tag="VHR42_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027221.1"
                     /translation="MKQFWTVFKFEFKNYAKNKSFIIVTALLVVVIGVVLSIPRIQQS
                     MSAGEAGKPEPTEKSVVALIDKTSGNANSAVSYLQSAISDTEFKPVSKSVDELKSAVD
                     SGTYQGAIIINSPLSYTYVVKNIGMYDDFSEKLDTALLAKYRSDKLTQLGVSQADAQS
                     FLNAKVQAETVQTSSGKDQMQNFFYTYILIIMLYMAILLYGQFVATSVATEKSSRAME
                     LLITTTEPKNLMFGKVLGAGLAGLTQFVLILGSSYLFYNINKSYFSDIKVIQSIFNMP
                     LSILLYTILFFVLGFFIYAFLYGALGSLVSRMEELSTAIMPVTMVFLAAFFIVIFSMA
                     GGNVDSVLMKICSYFPLTSPMAMFVRIAMGNVAGWEIAVSVVILVLSTAGIGFLAAAV
                     YRMGVLLYGKAPKPTEIIKMLKNSK"
     gene            complement(3397..>4181)
                     /locus_tag="VHR42_08355"
     CDS             complement(3397..>4181)
                     /locus_tag="VHR42_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3027222.1"
                     /translation="TIRIIMGVFPPDSGKVLMDGQPIHRDRVNIGYLPEERGLYPKKP
                     ILEQLIYFAELRGIGRKQAKDTAVHWLNRLEMGEYLNKRLDTLSKGNQQKIQLAATLI
                     SNPDFVILDEPFSGLDPVNAMLLKDVVKELIGAGKIVLFSSHQMNYVEEFCDSIAILN
                     QGNIVLSGNIKEIKRSYDRSKIVVASSQNSQISTFCTERLSDIVECSGEQGGELKIKM
                     KSADQKGALISALAEQGFDLDSVRVFEPSLNDIFVEYTEDAI"
BASE COUNT         1037 a         1043 c          978 g         1123 t
ORIGIN      
        1 taccgtcttt catttaagaa taggaaaatg ccttgagggc agccgcatgg gctgccctca
       61 ttttatttaa attttagtat tcctttcgcc cttgcggagc aaaaagggcg gctccgccgc
      121 cctaccccct cacgttagcg cgcgggtgat tcccctgtta ggggaaatgt tgcgaagcaa
      181 caaaagggcg gggcttcaca gaggcgcaat agcctagtag agcggaaaaa ttctaattaa
      241 ttttagtata acaataattc cgcaaaaagt ccacgttgca attattttgc tgcggtggtg
      301 acagcggcag cattcttata atcggccggc agagtcaaac caagagtttg gcaaaccgtt
      361 ttgttgtaaa caggagcata atcttttaca acatacaccg gcatatcctg cgcttttgct
      421 tcgcccttta atatcttcgc cgccattttg ccggtttctt tgccaagcgc gacataatcg
      481 atgctctgag atgcgaggca gccgttcttt acctgttcct cttcggagcc aaagacagga
      541 atctttttgg aattggcggc ctgaagcagt gtggaaaggt tgtttacgac gttattatcg
      601 gtaaaattat tgatgcagtc gacccctttt gaaacaaggg tctgcgcacc ggcggctacc
      661 tcggaagcgt tggttacgcc tacatcgaca atttcaaaat tgtatttcgg ggcaatttct
      721 ttgatttgct tgagctggga aacggagttt ggttcgcttg tggtgtaaag aataccgatt
      781 ttcttcgcgt tcggaaggaa cgcacggatc attttaacct gctgttccag cggaagaacg
      841 tcggaggagc cggtgcagtt atttccgggt ttgtcgttcg acttcacaat tcctgcggct
      901 accgggtcgg atactgctgt gaacacgacc ggaatattgg cggttctggc tgcactgaag
      961 gcagacattg cggacggggt agcgattccc atgataaggt catatttttt cgaaaccata
     1021 tttttggcta tcaggtcact gttggaaacg tcgccctggg cgttctgaaa atcaatggtg
     1081 aggtttgttc cctctttcag accggcttct tccagaccgg ccttaaagcc tttgaagcag
     1141 ttatcaagcg aaggatgcgg agcatactga atcacgccga ttttaaagct tttcgctgcc
     1201 tgacctgcgc ttgcggaagc tgcggttgaa gcggctgaag aagcggagct tgcggtaccg
     1261 gaagagcaag ccgttacgga tgctgccatc gccaaagcca tgaccgcgga aatcatcttt
     1321 ttaaatgtgt ttttcattat tatttcctcc taaagttaag cagtttattt tttgattcgg
     1381 gttccaattc atacctagcc atttacgcca tcgccaccag ccatctgcca taaaaccaac
     1441 tccttaactt aatttgagaa ataaaaaaag cccttgcccc tgaaaaacag ggacaagagc
     1501 attcactcct gcggtaccac ccggattgac gaaaaatcgt ccgcttgttc cgcacactga
     1561 ttatgtacgc ttcctttgta acgggtgaag ttcccgtcaa acactactcg gcgcatcgcc
     1621 tttcgttttg ccctcataag tccattcggc ctgccacatc ccaccgcaat ctcaccatct
     1681 gcggctctct gagtgaatga tatgcctgct tactactctt aatcatcggt ttgttttatg
     1741 tagctttatc gatattatat gcaatccgtt tgaaaatgtc aagagctctt tttcggtttt
     1801 gtaaggtcat agctctggcg tatccattca tgcagttcct cttccggaat cgcttcgtcc
     1861 ggcgtaaccg tgttccagtg caccttattc atatgatagg caggcgtaac ccccggataa
     1921 acgctgcgca gaaaatctgc caacatcggc tcgcatttca gattaatccg cagctttccg
     1981 ccgcgttcgt agatgagcgc gaagcttttt cggttcccgc tgtggcgcat gaccgtccac
     2041 gcctccgacg atgcggaatc ctcatcaaac ggataatcct catacgcatc ggggtaggtc
     2101 aggcagtatt caatcagctc tcgctttgtc atcggcttca cccgttttac ttgctgtttt
     2161 tcagcatttt aataatttcg gtcggcttcg gtgctttgcc gtacagcagc acgcccatgc
     2221 ggtacactgc agcggccagg aatccgattc ctgcagtgga aagaactaga atgactacgg
     2281 aaaccgcgat ttcccaccca gctacatttc ccattgcaat ccgtacgaac atagccatcg
     2341 gagaagtcag cgggaagtag gagcaaattt tcatcaaaac actgtctaca tttccgccag
     2401 ccatgctgaa gattacaatg aaaaacgccg caaggaacac catggtaacc ggcatgatcg
     2461 cggtgctgag ttcctccatg cggctgacaa gggaaccgag cgccccgtaa aggaacgcgt
     2521 aaatgaagaa cccgagaaca aaaaacagaa tcgtatacag caaaatagac agtggcatat
     2581 tgaaaatgga ctgtattact ttgatgtctg aaaagtacga tttgttaatg ttatagaaaa
     2641 gatagctgga gcctaaaatg aggacaaact gggtcagccc cgcaagcccg gcgccgagca
     2701 ccttgccgaa catcaggttt ttcggttcgg ttgtggtaat tagaagctcc attgcgcgcg
     2761 aacttttttc ggtcgcgacg ctggtcgcaa caaactggcc gtaaagcaga attgccatat
     2821 aaagcataat aatcaggata taagtataga aaaagttttg catctggtct ttgccagaac
     2881 tggtctgaac cgtttcagcc tgaaccttcg cattcagaaa gctctgggca tccgcctgcg
     2941 acacaccaag ctgcgtcagt ttgtcactgc ggtattttgc cagaagggcc gtatcaagct
     3001 tctcactgaa atcgtcgtac atgccgatat tctttacgac ataagtatag ctgagaggtg
     3061 aattgattat gattgcaccc tgataggttc cgctgtcgac cgcgcttttc agttcgtcta
     3121 cgctctttga aacaggttta aactccgtat ccgaaatagc ggactgaagg taggatacgg
     3181 ctgaattcgc attcccactg gtcttgtcaa tcaaagcgac aacgcttttt tctgtcggct
     3241 ccggctttcc ggcttctccg gcggacatac tctgctgaat acgcggaata gacagcacaa
     3301 cgccgattac aacaaccaag agcgccgtaa caataataaa gcttttgttt ttggcatagt
     3361 ttttaaattc aaacttgaat actgtccaga actgtttcat atcgcatcct ccgtgtattc
     3421 cacaaaaata tcattcagag aaggctcgaa cacgcgaacc gagtcaaggt cgaatccctg
     3481 ctccgcaagc gccgaaatca gtgcgccttt ttggtctgcc gatttcatct tgattttcag
     3541 ttcgccgccc tgttcaccgg aacactccac aatgtcggaa agccgttcgg tacagaaggt
     3601 ggaaatttga ctgttctgcg aagaagctac cacaatttta ctgcggtcat aagagcgctt
     3661 aatttctttg atattgccgg acagtacgat gtttccctga ttcagaatag cgatactgtc
     3721 gcagaattcc tcgacataat tcatctgatg gctggaaaac agcacgattt tccccgctcc
     3781 gatcagttct ttaacaacgt ccttcagaag cattgcattc acagggtcaa gaccagagaa
     3841 cggctcgtca agaatgacaa agtcagggtt ggaaatcagt gtggctgcaa gctggatttt
     3901 ctgctggttg cccttactga gggtgtcgag acgtttgttc aggtactccc ccatttcaag
     3961 acggttcaac cagtgcactg cggtatcctt tgcctgtttt ctgccgatac cccgcagctc
     4021 tgcaaaataa atcaactgct ccagaatcgg ctttttgggg taaagtccgc gctcttccgg
     4081 aaggtagccg atgttgaccc ggtcgcggtg aatcggctgg ccgtccatta gcactttgcc
     4141 gctgtccggc gggaacaccc ccataatgat gcggatcgtt g
//
LOCUS       DATALY010000309         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_67106,
            whole genome shotgun sequence.
ACCESSION   DATALY010000309 DATALY010000000
VERSION     DATALY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67106"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHR42_08360"
     CDS             <1..622
                     /locus_tag="VHR42_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3027223.1"
                     /translation="GAGRSETMQALFGLDKITGGSIELNGRPLTVQSPGQMIREGFAL
                     VPEDRRGEGIFAAHSLKFNLTLKVLREFIKGIHVDKNRENEITDTYMHSLSVKAANDQ
                     VAIGSLSGGNQQKVVISSWLAAHPKVLILDEPTRGIDVGAKSEIYAIMNELAKQGVSI
                     IMVSSELPEVLNMSDRIVVMCDGTITAIFDHSETSQEKIMQYAVKK"
     gene            645..1640
                     /locus_tag="VHR42_08365"
     CDS             645..1640
                     /locus_tag="VHR42_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027224.1"
                     /translation="MNETAKTKQNLGQKFINGCITYIKRNAGILIGLAIMCVIISVAS
                     PKFLTEKNLMNVLRQISSNLYLACGMTMILIVGGIDLSVGSVIAVIGVLGGTLINMNI
                     PLPLVVLICLAVGAFFGLISGGMIANTTLPPFIVTFSMMSILRGFAYVYTNGVTVRIN
                     DPGYLQMGTGYLGIVPLPVVYLIVILLIVYVIMSKTKLGRHLYATGGNDKAAKFSGIN
                     VKKVRLFVYIFSGVMAAFAGLMLSSRSYSGNPIAGNGAEMDAIAACVLGGVSFSGGYG
                     YIGGTIIGALIIGFLNNGLNLMGIGSFWQTILKGVVILAAVYVDYLKNLSKTADK"
     gene            1725..>1922
                     /locus_tag="VHR42_08370"
     CDS             1725..>1922
                     /locus_tag="VHR42_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEX3027225.1"
                     /translation="MAFIHTNFFSPTLGFNTDINVFIPTPNSDEILNHKASDYFYDGA
                     RFQLLYLLHGAYGDYTDWMRLT"
BASE COUNT          487 a          455 c          490 g          490 t
ORIGIN      
        1 cggagcggga cgcagcgaaa cgatgcaggc actgttcggc ttggataaaa tcaccggagg
       61 ctcaatcgaa ttgaacggca ggcccctgac cgtacaaagc ccggggcaaa tgatccggga
      121 aggctttgca ctggtaccgg aagaccgccg gggcgagggt atttttgcgg cgcacagcct
      181 gaaattcaat ttgacgctaa aggtactgcg cgagtttatc aaaggcattc atgttgataa
      241 aaatcgggaa aatgaaatta ccgataccta tatgcacagt ctttcggtca aagcggcaaa
      301 cgatcaggtg gcaatcggtt cgctttccgg aggcaatcag cagaaagtcg ttatttccag
      361 ctggcttgcc gctcacccga aggtactgat ccttgacgag cctacccgcg gaatcgacgt
      421 aggcgcaaaa agcgaaattt acgcaattat gaatgagctt gcaaaacagg gcgtttcgat
      481 tatcatggtt tcctccgagt tacccgaagt gctgaacatg agcgatagaa ttgtcgtaat
      541 gtgtgacggt acaatcacgg cgattttcga ccacagtgaa acatcgcagg aaaaaatcat
      601 gcagtacgcc gtaaaaaaat gacattggag aggtgtaaag agctatgaat gaaactgcca
      661 aaacgaaaca gaacttagga caaaagttta taaacggatg catcacttat attaagcgca
      721 acgcaggtat cttaatcggt cttgccatta tgtgtgtgat catttccgtc gcatcaccga
      781 aatttctgac cgagaaaaac ctgatgaatg tactgcgcca gatatcctcg aacctttatc
      841 tggcctgcgg tatgacaatg attctgattg tcggcggcat cgatctttcg gttggttccg
      901 tcattgccgt aatcggcgta ctgggcggca ccctgatcaa tatgaatatc cctctgccgc
      961 tggtcgtgtt aatctgcctg gcagtcggcg cgtttttcgg cctcatcagc ggcggaatga
     1021 ttgcgaatac cacgcttccg ccttttattg taaccttctc gatgatgagc attctgcgcg
     1081 gctttgccta tgtttataca aacggcgtga ccgtgcgcat caacgatccg ggctaccttc
     1141 agatgggtac cggatatctc ggcattgtgc cgcttccggt tgtatatttg attgtaatcc
     1201 tgctcatcgt ttacgttatt atgagtaaaa ccaagctggg gcgccacctt tacgcaaccg
     1261 gcggtaacga taaggcggca aagttcagcg gcatcaatgt caagaaagtc cgtctgtttg
     1321 tttacatttt cagcggagtt atggctgcct ttgccggtct gatgctttcc tcccgcagct
     1381 attcgggcaa cccgattgca ggcaacggcg ctgaaatgga cgcgattgcg gcctgcgtgc
     1441 tcggcggcgt cagcttttcc ggcggctacg gctatattgg cggaacaatc atcggcgctc
     1501 tgattatcgg ctttctgaac aacggactga atctgatggg aatcggatct ttctggcaga
     1561 caatcctcaa gggcgttgtt atcctggcgg ccgtttacgt tgattatctg aagaacttaa
     1621 gcaagactgc ggataagtaa cgggcagacg ccacacgaaa acgactttgt tttgtcagcg
     1681 acgctttgtt gttacgacag aaactggaaa aggaagaaaa agtcatggca tttattcaca
     1741 ccaacttttt ttcacccacg ctcgggttca atacggacat taatgtcttt attccaaccc
     1801 cgaattcgga tgaaatttta aaccataagg cgtcggatta tttttatgat ggcgcgcgct
     1861 ttcaactgct ttatctgctg cacggagcct acggagacta tacggactgg atgcggctga
     1921 cc
//
LOCUS       DATALY010000310         7746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_242844,
            whole genome shotgun sequence.
ACCESSION   DATALY010000310 DATALY010000000
VERSION     DATALY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242844"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VHR42_08375"
     CDS             <1..637
                     /locus_tag="VHR42_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX3027226.1"
                     /translation="AADIMIGFMAKIKQRLGIEFDELNLGGGFGIKYVASHDPIEYDR
                     YLEKISVRVKQACEKYGINLPFIYIEPGRSIVGPAGITLYEVGSVKEIPGVRTYVGVD
                     GGMNDNPRYALYQAPYEVLIANKAGEKRVKTVTVAGKCCESGDLIQENVAIQEPETGD
                     LMAVLATGAYNYSMASNYNRNPRPAVVMVKEGKPRIIVRPESYEDLIRNDV"
     gene            666..1196
                     /locus_tag="VHR42_08380"
     CDS             666..1196
                     /locus_tag="VHR42_08380"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX3027227.1"
                     /translation="MRKNQRPEPAGGDESPLLQSFKPISDGNSRILILGTMPSPASIR
                     AGMYYSHPQNAFWRLLSELTGDESGAGREERCAFLLRNRIAVWDTLRCCVREGAADSR
                     IRKPVYNDISGLLDGCPEIRAVFLNGGAAFRYYRKCGAAERFPGILLPSTSPANARGG
                     YLGKLERWRVILPFLK"
     gene            1324..1632
                     /locus_tag="VHR42_08385"
     CDS             1324..1632
                     /locus_tag="VHR42_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEX3027228.1"
                     /translation="MNSKIKSDHVDYLFESILSLKTKEECYNFFEDLCTIAEIKAMSQ
                     RLHVARMLREHHVYSDIVGQTGASTATISRVNRCLNYGSDGYNLILDRLKDMGEKDPS
                     "
     gene            1629..2378
                     /locus_tag="VHR42_08390"
     CDS             1629..2378
                     /locus_tag="VHR42_08390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3027229.1"
                     /translation="MKPYSFFAGCYDRLTRNVDYPRRAEYIREIFEHYRSEPKIVLDL
                     ACGTGSMSFELSKFGYDMIGVDSSGDMLAQASERASLCGSDILFICQDMCGLDLYGTV
                     DAAICCLDSVNHLTSAAKVQAAFEKVSLFLNPGGLFVFDLNSEYKLSEVLGGNTFLYD
                     MGDLYCIWENHYNKRSKLCRFDLTFFQYENGGFQRYDEQFGEKAYSSARISEFCSRAG
                     MNLIGEFNELSFEKPHPQSERIYYVAEKRRN"
     gene            2385..3284
                     /gene="hslO"
                     /locus_tag="VHR42_08395"
     CDS             2385..3284
                     /gene="hslO"
                     /locus_tag="VHR42_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX3027230.1"
                     /translation="MSSKMIRAITSDGSMVAFAIDSKEITNRACEIHKPSAVVIAALG
                     RVLSAASMMGVMLKGSNDSVTIRINGGGPSGPLIAVSDGLGNVRGYVANPVVELPLNE
                     YGKLDVAGAVGKNGTVNVIKDLNLKEPYSGQTPLVSGEIAEDITNYFAKSEQIPTVCA
                     LGVLVDTDLTVRASGGYIIQLLPFCDSSVIDRLEQNLKEMPPVSSLLDAGRTPDEIIR
                     MVLKGFDVEIMEETDVNYVCSCSRDRIARALKSLGVKELSTILDEQGEAEVTCHFCDK
                     KYQFTKGDLKEMIQEIGNDVEKS"
     gene            3399..3474
                     /locus_tag="VHR42_08400"
     tRNA            3399..3474
                     /locus_tag="VHR42_08400"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3432..3434,aa:Val,seq:tac)
     gene            3478..3554
                     /locus_tag="VHR42_08405"
     tRNA            3478..3554
                     /locus_tag="VHR42_08405"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3512..3514,aa:Asp,seq:gtc)
     gene            3560..3635
                     /locus_tag="VHR42_08410"
     tRNA            3560..3635
                     /locus_tag="VHR42_08410"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3593..3595,aa:Phe,seq:gaa)
     gene            3641..3716
                     /locus_tag="VHR42_08415"
     tRNA            3641..3716
                     /locus_tag="VHR42_08415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Gly,seq:gcc)
     gene            3868..3942
                     /locus_tag="VHR42_08420"
     tRNA            3868..3942
                     /locus_tag="VHR42_08420"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3900..3902,aa:Cys,seq:gca)
     gene            4078..4153
                     /locus_tag="VHR42_08425"
     tRNA            4078..4153
                     /locus_tag="VHR42_08425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4111..4113,aa:Thr,seq:cgt)
     gene            4478..5437
                     /locus_tag="VHR42_08430"
     CDS             4478..5437
                     /locus_tag="VHR42_08430"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX3027231.1"
                     /translation="MDLNKEKTHVKLIFNPGSGENLDSPVQLTSIINELQASGFIAET
                     YLINSDSNLSEEVRDAIKQGIEMFVVCGGDGTVSSAARELYGKNVTLGIIPTGTQNNI
                     AFSLGIPSDIPSAISLLQTGLRKKVDVGLVNCGENHISFLEVCSVGLFSSIFSSADDI
                     QHGNLLRIGDFLTELVTTQPAEIHLLLDNEREILTTGHVMLISNMPYVIRHFQVGSAT
                     SFNDGMLDVLLFANMSKLDLVGYAINGQRLDQSDDPRIHRFRIRKAEIYTDPAMPVMA
                     DGIDLGEGRITVEVQKSALSVMTPCPAPKATEKPGDRLEKESE"
     gene            5418..6218
                     /locus_tag="VHR42_08435"
     CDS             5418..6218
                     /locus_tag="VHR42_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3027232.1"
                     /translation="MKKNPNNQEIKPLPEVRIPGTLGRAAHWIAHIRPIYWLFIVVSA
                     LALCSIFSPRTIWAVLKEAIRAQKYLVVSLAIFSLLAISLIWKTGQRIDVWVFQYINN
                     RGHRPPWLDGLMLGLTQIGNGVFAYLVAFVLYLRVKHLLAYELIFGNLTLWLTVELIK
                     ILIRRSRPYSMLDKTRVVGNRERGTSFPSGHTSQAFFMASLFAHYYYTGFCLTFLLYA
                     IALFVGITRMYVGMHYPRDVLGGVILGSAWGILGMIINSYIFTQFNIH"
     gene            6662..>7746
                     /locus_tag="VHR42_08440"
     CDS             6662..>7746
                     /locus_tag="VHR42_08440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3027233.1"
                     /translation="MSTYQVSDKSVKHSLNLTNFAITFGMVFYSIFGQPVGSSLFTGF
                     MRKLGTNDLVYSVVMALPVLGGVSQILGSYFLERTGKRRFLFLASGFVHRLFWIPVAL
                     IPLFIVSDMHQAIIISITILITVSSVAYSVNTVVFNSWMGDLVPQEIAGRFFSTRSLI
                     SNISGGIAALLVGAFIDRVNNLNGFAIIFILGVLFGMVEIITFFRVKNPPMTLSESKP
                     SLVHIIIEPFKNKNYMKFVAFATIFMFGVNFAVPFFNVYMIEKLKMSYFIIAMANSVT
                     YSIGAVVFVRKWGALADRFGNKPVVFCCALGVAIVPIIWLFAAPGHILMTYVANFVAG
                     VAWSGYYVAIFNQSVWLAPEKNRSAYIA"
BASE COUNT         1935 a         1693 c         2035 g         2083 t
ORIGIN      
        1 tgcggcggat atcatgatcg gttttatggc gaagatcaaa cagcgtttgg ggattgaatt
       61 tgatgaactc aatctcggcg gagggtttgg catcaaatat gtcgcttcac acgacccgat
      121 cgaatatgac cgctatcttg aaaaaatatc ggtgcgggtc aaacaagcct gtgaaaaata
      181 cggtatcaac ctgccgttta tctacattga gccgggcaga tccattgtcg ggccggcggg
      241 tattactctt tatgaagtcg gctcagtgaa agagattccc ggagtccgca cctatgtggg
      301 tgttgacggc ggaatgaatg acaatccgcg ctatgcgctt tatcaggcgc cttatgaagt
      361 gctgatcgcg aacaaggccg gcgaaaaacg tgtcaaaacg gtcacggtgg cggggaaatg
      421 ctgcgagagc ggcgacctga ttcaggagaa tgtggcaatt caggaacccg aaaccggcga
      481 tcttatggcc gtgctcgcga ccggagctta taactattcc atggcttcca actataaccg
      541 gaatccgcgc cctgcggttg ttatggtgaa agagggtaag ccccgcataa ttgtccgacc
      601 ggaaagctat gaggatctga tccgtaacga tgtataagtg gaatgactcg ggggcggttg
      661 aattgatgcg gaaaaatcaa agacccgaac ctgccggcgg cgatgaatcg ccgcttttgc
      721 agtcttttaa gcccatcagc gacgggaaca gccgaatcct gattttggga accatgccgt
      781 cgcccgcttc gattcgggcg ggcatgtatt attcccatcc tcagaacgcc ttctggcgtc
      841 tcctgtccga gctgacagga gacgaatccg gggcgggcag ggaagaacgc tgtgcttttt
      901 tactgcgtaa tcgcatcgct gtgtgggata cgttgcgttg ctgcgttcgt gagggggcgg
      961 ctgacagcag gattcgaaag ccggtatata atgatatttc cgggctgctg gatggctgcc
     1021 cggaaatccg ggccgtcttc ctcaatggag gggcggcttt ccgttattac aggaaatgcg
     1081 gcgcggcaga acgctttccg ggtattcttc tgccttctac cagccctgca aacgccagag
     1141 ggggctacct gggaaaactc gagcggtgga gggtgattct gccgtttctc aagtgacccg
     1201 gagctgtttc ggcgcaggct cctccattta cttttacgat ttattgtgtt aaaattggag
     1261 cagagaaagc aataggtgga tcactgaaaa ctgtcaattt tgattcatcc gggggcgtaa
     1321 atgatgaaca gtaaaatcaa aagcgaccat gtggattatc tgtttgaatc gatactttca
     1381 ctgaagacaa aagaagaatg ctataatttt tttgaagatc tttgcacgat tgctgagatc
     1441 aaagcaatgt ctcaaaggct gcatgtggcc agaatgctgc gggagcatca cgtttacagc
     1501 gatattgtcg gccagaccgg agcgagtacg gcgaccatca gcagggtgaa ccgctgcctg
     1561 aattacggaa gcgacggtta caacctgatt ctcgaccggc tcaaagatat gggagaaaaa
     1621 gatccgtcat gaaaccttac agtttttttg caggctgcta tgaccgcctg acccgaaatg
     1681 tggattatcc caggcgggcg gaatatatca gggaaatttt tgaacactat cggtcggaac
     1741 cgaaaattgt tcttgatctt gcctgtggaa caggcagtat gtccttcgaa ctgtcgaaat
     1801 tcggttacga catgatcgga gtggactcgt ccggcgacat gctggcccag gcgtcggaaa
     1861 gagcgagttt atgcggctcc gatattcttt ttatctgtca ggatatgtgc gggcttgacc
     1921 tttacggaac ggttgacgcc gcaatctgct gccttgacag cgtgaatcat ctgacttccg
     1981 ccgcgaaggt tcaggcggcg tttgagaagg tctcgctgtt tttaaatccg ggcggtcttt
     2041 ttgtgtttga tctgaattcc gaatataagc tttccgaggt tttgggcgga aatactttcc
     2101 tatatgatat gggggatctt tactgcatct gggaaaacca ttataataag agatcaaagc
     2161 tatgccggtt tgacctgacc ttttttcaat acgaaaacgg cggcttccag cgttacgatg
     2221 agcagttcgg tgaaaaggcc tattcctccg cccggatttc cgagttttgc agccgggctg
     2281 gaatgaatct gatcggggaa ttcaacgaat tatcatttga aaagccacac ccccaaagcg
     2341 agcggatcta ttatgtggcc gagaagagga gaaattaaca gtttatgagc agcaagatga
     2401 tccgggcgat aacgtccgac ggcagcatgg ttgcctttgc cattgattca aaagagatta
     2461 caaacagggc ctgtgagatt cataagcctt ccgcggtagt catagctgcg ctgggcagag
     2521 tgctctcggc cgcgtcgatg atgggcgtca tgcttaaggg cagcaacgac agcgtcacga
     2581 tccgaatcaa cggaggcgga ccttccgggc ccttgatcgc ggtgtccgac ggtctgggca
     2641 atgtgcgcgg atatgtggcg aatcccgttg tggaactgcc actcaacgaa tatggcaagc
     2701 ttgacgtggc aggagctgtg ggaaaaaacg gaacggtcaa tgttatcaaa gacctgaacc
     2761 tcaaagagcc ttacagcggc cagaccccgc ttgtttcggg cgaaattgcc gaggatatta
     2821 cgaactattt tgcaaaaagc gagcagattc cgactgtttg cgcgctcggc gtacttgtcg
     2881 atactgacct tactgttcgc gcttcaggcg gctatatcat tcagctgctg cctttctgtg
     2941 acagcagtgt gatcgaccgg cttgagcaaa atctgaaaga gatgccgccc gtgtcgtcgc
     3001 tgctggacgc gggaagaaca ccggatgaga tcatccgaat ggttcttaaa gggtttgacg
     3061 ttgaaattat ggaagaaact gatgtgaatt atgtctgcag ctgctcaagg gacaggatag
     3121 cccgggcttt aaaaagcctt ggcgtaaaag agctttccac gattctcgac gagcaggggg
     3181 aggccgaggt cacctgtcat ttttgcgata agaaatatca gtttaccaaa ggcgacctca
     3241 aggaaatgat ccaggaaata gggaatgatg tcgaaaaaag ttgagtgaca ggcggaaaat
     3301 aaaataaaaa gtttccccta tgtgttgaca agaggctgct tttatagtaa aataatcttc
     3361 gccgcttcaa gggaagcaac gggataatat cccaaaacgg gagcatagct cagctgggag
     3421 agcacatgcc ttacaagcat ggggtcatag gttcgagccc tattgttccc accaaacggc
     3481 ccgatagttc agttggttag aacgctagcc tgtcacgcta gaggtcaggg gttcgaaccc
     3541 ccttcgggtc gccaattttg cctctgtagc tcagtcggta gagcagggga ctgaaaatcc
     3601 ccgtgtcggt ggttcgattc cgcccggagg caccaaaaac gcggatgtag ctcatctggt
     3661 agagcgccac cttgccaagg tggaggtagc gagttcgagt ctcgtcatcc gctccattta
     3721 cccggcagca gaaagctgac cgaataaagc tgcggttgag aggttggtta aattgccggc
     3781 tgatgggtcc gcgctttatt ttttgtatgt caggcgtttg gcggatgaaa tcaggacgat
     3841 ggatcatgca ggcgggtaat ttcatatggc gccatagcca agcggtaagg cagaggtctg
     3901 caaaacctct attccccggt ccgattccgg gtggcgcctc cattttgctc atgtagctta
     3961 ttccggcaga gaagcgcaca tgtatgcgca gatctccggt tgcagctgaa aaatataagt
     4021 tggcagacat tattacctcc aaaacgaggg aacggcaggt tccgtcctct cacatatgct
     4081 agtgtggctc agttggtaga gcagcgcact cgtaatgcgc aggtcagcgg ttcgaatccg
     4141 ctcactagct ccaagaagaa tgacttacct ctagggttta agccattctt tttttgttgc
     4201 cagtgtggca aaaagggcga aaaaagaaga cagaaaacca aaacttaaaa aaatccaaca
     4261 taaataacca gagatcagcg gtttaagacc gtttaccatc ttattcagaa cccccggcaa
     4321 gccgggggtt ctgaatatct caaaggactt ttccgcagat ccaatcgaaa aagaagacaa
     4381 gagtaaaaca agtgaaaacg aggaatgcta tagaataatg tttgtatggg ttgttttaag
     4441 aacatattcg aattaacaaa gaaaagggct attttagatg gatttaaata aagaaaaaac
     4501 acatgtgaaa ttaattttta atccgggctc aggtgaaaac cttgattcgc ccgttcagct
     4561 tacaagtatt attaatgaat tacaggcttc agggtttatt gctgaaacct atctaatcaa
     4621 ttctgacagc aacctttccg aagaggtccg ggatgcaata aaacagggaa tcgaaatgtt
     4681 tgtggtctgc ggcggtgacg gtacggtttc ttctgcagcg cgagaactgt atggaaaaaa
     4741 cgttacactt gggattattc ctaccggaac tcaaaataat atagctttca gtctgggtat
     4801 tccaagtgac attccctctg ctatcagcct gttgcagaca ggcctccgta agaaagtaga
     4861 cgttggcctg gtgaattgtg gggaaaatca tatttccttt ttggaagttt gctctgtggg
     4921 attgttttca tctatctttt cgtctgcgga cgatatacag cacggtaact tgttaaggat
     4981 cggagacttt ctgacggagt tagtcacaac ccaaccggcc gagatccacc ttttgcttga
     5041 taatgaacgg gaaatcctga cgacaggcca tgtgatgctg atttccaata tgccctacgt
     5101 aatacgccat ttccaggtgg gttcagcaac gtcttttaat gacggcatgc tggatgtttt
     5161 gttgtttgcg aatatgtcaa agcttgatct ggtgggatat gcaatcaacg gacagcgctt
     5221 ggatcaatcg gatgatccgc gaatccatcg gtttcgcatt cgtaaagcgg aaatctacac
     5281 cgatcccgcc atgcctgtca tggcggatgg aattgacctg ggggaaggaa gaataactgt
     5341 tgaagtgcag aaatcggcct tgtcggtgat gaccccatgt ccggcgccaa aggcgactga
     5401 aaaaccaggt gatagacttg aaaaagaatc cgaataatca ggaaatcaag ccactgcctg
     5461 aggtaagaat tcccgggacc ttggggaggg cggcgcattg gattgcccat attcgaccga
     5521 tttactggtt gtttatagtc gtttccgccc tggccttatg ttcgatattt tcacctcgca
     5581 ccatttgggc agtgctaaag gaagccattc gggctcagaa gtatttggtg gtttcactgg
     5641 ccatcttcag tctgcttgcc atttcgctga tctggaaaac cgggcagcgc atcgacgttt
     5701 gggtttttca atatattaac aatcgggggc atcgtccgcc atggcttgac ggcttgatgt
     5761 tggggcttac tcagatcggt aacggtgtct ttgcctatct ggtcgccttt gttttatatt
     5821 tgcgtgtaaa gcacctgctg gcatatgagc ttatatttgg aaatttaacg ctgtggctga
     5881 ctgttgagct aatcaagatc cttattcgaa ggtctcggcc atactcgatg ctggataaga
     5941 cccgggttgt tggcaatagg gaaagaggga cttcatttcc aagcgggcac accagccagg
     6001 ccttttttat ggcgtcgctg tttgctcatt attactacac cggcttttgc ctgacctttc
     6061 ttctgtatgc catcgcttta tttgtgggga taacccggat gtatgttgga atgcactatc
     6121 ctcgcgatgt gttgggcgga gtgattttag gttcagcctg gggcatttta ggcatgatta
     6181 taaacagtta tatttttacc caattcaata tccattaggt tcacatgagc cgccttgatt
     6241 agagcagagt aacaccatgt tacgggacgg cggcagatag agtttttgat tgcgcttgtg
     6301 tgaaaattga tgagcagctc cgccgaaaaa tcgttttgca tatttgcgga acggccttga
     6361 ctgcctctga caattactat ataatgaaaa tcacggtttt ttgccatccg ttttgttaag
     6421 ggatggcggg atgagaagca atgatcgaat cagatgtaga atagaatggc ttgccattta
     6481 tggcttgcca tttttgtttg aatttttttg tgttgtataa aaaccagaaa ttacaaaaaa
     6541 tgaggagcag gttttacttt attcttaaat tcttattctt gcgccggtac agcccgaaaa
     6601 ggttgctcct gacgggggat cgggcgaaga tttcttcaat cattcagata agggggattc
     6661 tatgtctacc tatcaggtgt cggacaaatc ggtcaaacac agcttgaatt taacgaattt
     6721 tgcgattaca ttcggaatgg ttttttactc gatttttggc cagccggtcg gctcctctct
     6781 ttttacgggt tttatgagaa aactgggaac aaacgatctg gtttattcgg tcgtgatggc
     6841 cctgcctgtt ttgggcggtg tatcacagat tttgggatcc tattttttgg aacgaaccgg
     6901 aaaaagacga tttttatttc tggcatccgg ttttgttcac cggcttttct ggattccggt
     6961 tgccctgatt cctctgttta tcgtatccga catgcatcag gccattatca tttcgatcac
     7021 catcctgatc acggtatctt cggttgccta ttcggtcaat accgtcgtgt tcaactcctg
     7081 gatgggcgac ctggttccgc aggaaatcgc cggcagattt ttcagcacaa gatcgctgat
     7141 cagtaacatt tcgggcggaa tagcggcgct tttagtcggc gcattcatcg accgggtcaa
     7201 caatctcaac ggctttgcca tcatttttat tctgggcgta ttattcggca tggtggaaat
     7261 catcactttt ttcagagtga aaaatccgcc tatgacactt tccgagtcca aaccctcgtt
     7321 ggttcatatc atcatcgagc cttttaaaaa taaaaattat atgaagtttg ttgcgtttgc
     7381 gacaattttt atgtttggcg tgaatttcgc tgtccctttt ttcaatgttt atatgattga
     7441 aaaactcaaa atgagctatt ttatcatcgc catggcgaac tcggttacat atagtatcgg
     7501 ggcggtggtg ttcgtaagaa aatggggtgc gctcgctgac cggttcggca acaagcctgt
     7561 ggtcttctgc tgtgcgctcg gggttgcgat tgttccaatt atctggctgt ttgccgcgcc
     7621 gggccatatt ctcatgactt atgtcgccaa tttcgtagcc ggcgtggcat ggtcgggcta
     7681 ttatgtagct atttttaacc aatcggtttg gcttgctccg gaaaaaaacc gctcggcgta
     7741 tattgc
//
LOCUS       DATALY010000311         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_841798,
            whole genome shotgun sequence.
ACCESSION   DATALY010000311 DATALY010000000
VERSION     DATALY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841798"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VHR42_08445"
     CDS             complement(<1..157)
                     /locus_tag="VHR42_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3027234.1"
                     /translation="MAELIISNLTKKFGDFTAVSEISFSVQNGKMIALLGGSGCGKTT
                     TLRMIAGF"
     gene            complement(202..1002)
                     /locus_tag="VHR42_08450"
     CDS             complement(202..1002)
                     /locus_tag="VHR42_08450"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027235.1"
                     /translation="MNERKLNLPLKIFACLVFIFLMLPLIVVILASFSPTALVMFPPK
                     GFSLTWYRNIFNSQTNFMSGFADSMLVGITATVIDVFLGITAALSVGRYKFKGRSFLS
                     ALFSSSMYVPSITFAFVLLQIGSAVNGISSFAQILVGHAVIIFPYIVRNVIATLSGYN
                     WELEDAAASLGASPIHTFFRVTLPIIQPGVISGAILAFLFSFDEAVLSNLLSSPTFVT
                     LPVRIMNYMEFSFDPTLAAISTILILLSLAITLTAEKFVGLKIMFKDR"
     gene            complement(995..1810)
                     /locus_tag="VHR42_08455"
     CDS             complement(995..1810)
                     /locus_tag="VHR42_08455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027236.1"
                     /translation="MLSAFPAVLALILFFILPLLLILVSSLQADGLKYYQKFFSDGFY
                     LDILWTTVWISLAVMLITLLVGYPTAYYLARTQSKFKNLLMIVVIFPFLVSSVVRSYG
                     WMVILGNNGLLNQFLLACGVIHSPLVIMNTNKAVIIGLVHLLLPYMIFSVTSILNTID
                     DNVELAASSLGCGPVATFFKVTLPLSSPGAISGCIIVFILSMTSYVTPQLLGGSKFRM
                     MSTMIYQQINVEFNWSFACALSYILMGIILLFLVVSNVMTSGLNKRVGGEKRE"
     gene            complement(2256..2768)
                     /locus_tag="VHR42_08460"
     CDS             complement(2256..2768)
                     /locus_tag="VHR42_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027237.1"
                     /translation="MKRLLLVFMSAVFLIPTACSQAASPVSASGVSSEGAVRPVTFDA
                     VPGSSESSAAAAGDAVTLPGSLFVGQNLDDIKADAQKSGVESVVQNKDGSVTYKMTKD
                     AHQQLLINTRQKTIRDLQKFQNNSDFPFIRSLLYSDDFSEITLVVDKSQYDTVKGSDD
                     LCDLSLLTAI"
     gene            complement(2810..3070)
                     /locus_tag="VHR42_08465"
     CDS             complement(2810..3070)
                     /locus_tag="VHR42_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027238.1"
                     /translation="MNGAEELLACQEYFKNSKYPAANDLEDVYRFYCPKADLHLDISK
                     RVMEFACYDADGMIGIQIRRVCAAQCRYYQEMVRKSKFVVLK"
     gene            complement(3185..3337)
                     /locus_tag="VHR42_08470"
     CDS             complement(3185..3337)
                     /locus_tag="VHR42_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027239.1"
                     /translation="MTRFHEADARIHSIGGSIIYDARLRKYYIAPIRGSELDRLIREF
                     SAEKAD"
BASE COUNT          997 a          882 c          806 g          804 t
ORIGIN      
        1 taaagcccgc aatcatgcgc agggttgtcg tctttccgca gccgctgccg cccagaaggg
       61 cgatcatttt cccgttttga accgaaaacg atatctcgct tacggcagta aaatcgccga
      121 attttttagt cagattggag ataatcagtt ccgccatatt acgacccttt ctttttctgc
      181 attgataacg aaaacacccg gttaccggtc tttaaacata attttcagcc cgacgaattt
      241 ttctgccgta agcgtaatcg caagcgacag aagaatcaga attgttgaaa tggctgcaag
      301 agttggatca aacgagaact ccatatagtt cataatgcgg accggcagag tgacaaaggt
      361 tggggaactg agaagattgc tcagcaccgc ttcgtcgaag gagaacagga acgcgagaat
      421 cgcccctgaa atgacacccg gctgaatgat cggcagggtc acccgaaaaa acgtgtgaat
      481 cggcgaagcc cctaggcttg cggccgcatc ctccagttcc cagttgtagc cggaaagcgt
      541 cgcaataacg ttccgcacga tgtacgggaa aataatgacg gcatgtccca caaggatctg
      601 cgcgaacgaa gaaattccgt tgacggcgct tccaatctgc agaaggacga aagcgaacgt
      661 gatagaagga acatacatcg acgacgaaaa aagcgccgaa agaaacgagc ggcctttaaa
      721 cttgtagcgc ccgacgctca aagccgccgt aattcccaga aaaacatcga ttaccgtcgc
      781 cgtgatgccg accagcatgc tgtcggcgaa gccgctcatg aaattcgtct gtgaattgaa
      841 aatgttccgg taccacgtaa gagaaaagcc cttcggtggg aacatgacca aagcggtcgg
      901 actgaacgac gccagaatta cgacaatcag ggggagcatc agaaaaataa aaacaaggca
      961 ggcaaaaatt ttcaagggca agttaagctt gcgctcattc acgtttctca cctccaaccc
     1021 gtttgttcag cccagaagtc atcacattcg aaaccaccag gaacaacaga atgatgccca
     1081 tgaggatata gctcagcgca caggcaaagc tccagttgaa ctcgacattg atctgctgat
     1141 agatcatggt gctcatcata cggaatttgg aacctcccaa cagctgagga gtcacgtaag
     1201 aagtcatgct taaaataaaa acgatgatac agccggatat ggcgccggga gagctgagcg
     1261 gaagtgtgac cttaaagaaa gtcgcaacag ggccacatcc gagactgctc gccgccaatt
     1321 cgacattatc atcgatggtg tttagtatac tggtaacgga aaaaatcatg tagggcaaaa
     1381 gcagatgtac gaggccgata atgacggcct tattggtgtt cataatcaca agcggactgt
     1441 ggatcacacc gcaggcaagc agaaactgat tcagcagacc attgttgccc aaaatgacca
     1501 tccagccgta ggaccgtacg acggaactga caagaaacgg aaaaatgacg acgatcatca
     1561 gcagattttt aaacttcgac tgcgttctcg ccagataata agccgtcgga tatcccacca
     1621 gcagcgtaat caacatcaca gcaagagaaa tccaaaccgt tgtccacaaa atatccaggt
     1681 agaacccgtc gctgaaaaat ttctgataat atttcagacc atccgcctgc aaagaagaaa
     1741 ccagaatcaa aagcagtgga aggataaaaa acaaaatcag cgccagcacc gccgggaaag
     1801 cagacaacat tattcttctc tttcgactca tccacaaccg tcccttcagc tataacaaaa
     1861 aacacctgcc gctatttccc atagaaacga aaataacgga aagtgttttc tatcaggaat
     1921 catgctacaa cggaatacaa aaactgtttt tccagtgtta ttcaaaatta acagaaacgg
     1981 agcccgttct ttggagcttc cttattttat tcagtctata gttttgtgca gattttgtca
     2041 agtacttact ctataaactg tcacccgacg acggcagaac tgctcacgcc accggagctt
     2101 accgcagagc tgcctgcagt gccggaactc ttcaaatcca tcggataaag cgccgtttta
     2161 aacagctgat tcgtttcccg gtcacggtat ctcagcgtgg tttttgccga cccggcactc
     2221 gcgccgccga tgatctgata agcggaaacc gcgtttcaga tcgccgttaa aagcgacaaa
     2281 tcgcaaagat catcagaccc tttcactgtg tcgtactggg atttgtcgac caccaaagta
     2341 atttccgaaa aatcatcgct atatagaaga ctgcgaatga acggaaaatc actgttgttc
     2401 tggaatttct gcagatcccg gattgttttc tgtctggtat tgatgagcag ctgctgatgg
     2461 gcgtccttcg tcattttata ggtgacggag ccgtccttgt tttgaaccac gctctcaacc
     2521 ccgctttttt gggcgtccgc cttgatgtcg tcgaggttct gccccacaaa cagcgaaccg
     2581 ggaagtgtta cagcatcgcc ggccgcggct gcggagcttt ccgaagaacc cggcaccgcg
     2641 tcgaaggtta ccggtcggac tgccccctcg gaggaaacac cggaagcgga aaccggagaa
     2701 gccgcctgag agcaggctgt cgggatcaga aataccgcac tcataaaaac aagcaaaagt
     2761 cgtttcattg tttcctccag attttactgc atcccttaat tttttgttct catttcagca
     2821 cgacaaattt tgattttcgg accatttcct gataatacct gcactgagcg gcacagacgc
     2881 gccggatctg tatgccgatc atgccgtcgg catcgtagca cgcaaactcc ataacccgct
     2941 ttgaaatatc aagatgaagg tcggccttcg ggcagtaaaa acgataaacg tcttccaagt
     3001 cgtttgcagc cggatacttg ctgtttttaa aatattcctg gcaggccaag agttcttctg
     3061 caccgttcat ctgttcatca cctgcaccta ttataaagcc gggctgccgg aaatcaagga
     3121 ttttcaatcg atttgtaaag aagcttctta ttttctattg ccaacagaca ttggttaacg
     3181 tgctttaatc tgccttttct gcggaaaact cccggatcag ccggtcgagc tcggaacccc
     3241 gaatgggcgc aatatagtat tttcgcagcc gggcgtcgta gatgatactt cctccgatgg
     3301 aatgaatccg ggcgtccgcc tcatgaaaac gtgtcacgtt ccgcagggca atgcggaggt
     3361 actctttgag ccttttccgc accggaatag gtgaacatcg gcggaaacag gatggttttt
     3421 ttctcaatga ctgcataatt gatgaatcga cccgcatcat gaaatccgtc gtccgttttc
     3481 aagcttttc
//
LOCUS       DATALY010000312         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_672861,
            whole genome shotgun sequence.
ACCESSION   DATALY010000312 DATALY010000000
VERSION     DATALY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672861"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VHR42_08475"
     CDS             <1..501
                     /locus_tag="VHR42_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX3027240.1"
                     /translation="FLKGKSFENIRNRVNKFHREHPEAEIRLFEADHYDDLLTLGKTW
                     KDTSGKKYSHVYDGVYYKQLVKHYRELNQKILTVRIDGKLVGMISGSGLPNGQAWGSV
                     IKFAQGIPGLSETLTVEFAKLLYKPKGGPEFLNVGSDLGFEGLKMFKLKFRPVLNYRR
                     YELFLK"
     gene            546..932
                     /locus_tag="VHR42_08480"
     CDS             546..932
                     /locus_tag="VHR42_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027241.1"
                     /translation="MAGILRSRMIQPKGCFAGGAGVPVRQVWDYRREERSMSGESCGF
                     DGIIRAFHDEEKTLRRLAGIEEELLKNAQSSSGNLKEYIAVHHAVRKTLRQTRSLARL
                     LRKKRQAAENLRKSGEKITETDELEE"
     gene            951..2087
                     /locus_tag="VHR42_08485"
     CDS             951..2087
                     /locus_tag="VHR42_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheC/YheD family protein"
                     /protein_id="HEX3027242.1"
                     /translation="MNEGIRIMWAKVEITHSDEDLIYLPLSLTGEYREHIQIKFGGLN
                     VRVKVRYADGAGLEGSTYDRPGALILSDHLVEKLMIPRAPVYQIKIREGNLRIGPVIG
                     FLLGSQTQRYSPEHMEKYSDRFGIYHKVGGLIYAFSPRSIEWKKNAAFGLYYNIEKSK
                     WEYGKFPLPETIYRRDFHSDPEVIEKLIRYTHGRLFNSYRFDKYELYRFVGQNEELKK
                     YLPPTEIIESAGQVSDFIGRFPCSIIKPVDLSRGRGILVVETNEGGYKITDYRQKDPV
                     VEHLDGDSMLNAFFERNTELFSQHIIQKYLPLASIGSSIFDVRVVMQKRVNHSWGCSG
                     IECRVSNDDCLLTNISKGGYALTLKHALHKSFPDNGRQIPKKNS"
     gene            2029..2328
                     /locus_tag="VHR42_08490"
     CDS             2029..2328
                     /locus_tag="VHR42_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheC/YheD family protein"
                     /protein_id="HEX3027243.1"
                     /translation="MRFINRFPITADRFRKKILDLCRKICVYLDTSGHHYAELGIDIA
                     IDRDQNLWLIEANVFPSFKGFKKIDRETYLRIRYSPMIYARSLTGFENSGGEKHV"
     gene            2321..2935
                     /locus_tag="VHR42_08495"
     CDS             2321..2935
                     /locus_tag="VHR42_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027244.1"
                     /translation="MCETVKIRQMEGGRNILAMNPSQAASLHFEFGKTAEVTFGNLSR
                     SAQLRMNKNVPPNEVWMSGRLLSGLRIPDYPVYELRAEASRLTVGPCIGLLLCSKSSR
                     FTDEWLGRLGVYLSDYDLLHGAVLAFALDQIDTEKKLIRGYCYRPSDQSWVKGVYPYP
                     CAIYRRIGLSDRWKRHQPIIFYETYSYGVLTSSSRVAKRQTPGF"
     gene            3101..>3676
                     /locus_tag="VHR42_08500"
     CDS             3101..>3676
                     /locus_tag="VHR42_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX3027245.1"
                     /translation="MTALLLTFFCIAVILVMLVIFKKPADISGITGLIVISLIAMLFF
                     HTSLEVILRSAAAGFVKSFSVSLIVATSLLQRALIEKSGALKRIIIFIKTLSPENRAA
                     QIMLINIGFGTLMVAVGATPVSLLPPILLAMGYSTYVAIALPSIGYDSLCTYALLGAP
                     IVVFVDMANGFLKNSGIHTEITLSQVGQIFAY"
BASE COUNT          969 a          824 c          932 g          951 t
ORIGIN      
        1 tttctgaaag gaaaatcatt tgaaaatatt cgaaacaggg tcaacaaatt tcaccgggag
       61 catccagagg ctgaaatccg gctttttgaa gcggatcatt acgacgacct tctgacgctg
      121 gggaaaacgt ggaaagacac ctcgggtaaa aaatattctc atgtttatga cggcgtttat
      181 tataaacagc tggtcaagca ttacagagaa ttaaaccaga agatcctgac cgtccggatc
      241 gacggaaagc ttgtgggaat gatttcaggc agcgggctgc caaacgggca ggcctgggga
      301 agtgtcatca agtttgccca gggaattccg ggattgtctg agacgctgac ggtcgaattt
      361 gcgaagttgc tttacaagcc caaaggcggc ccggagtttt taaatgtcgg cagcgatctg
      421 ggctttgagg ggcttaaaat gttcaaactg aaattcaggc cggttctcaa ttacaggcgc
      481 tatgaacttt ttttgaaata acgcgccccg aagtgccact cagccggtct tcatctccgg
      541 atgccatggc gggaattctg cgctcccgaa tgattcagcc gaaaggctgt ttcgccggcg
      601 gcgcaggcgt ccccgtcagg caggtctggg actacaggcg ggaggaacgg agtatgtcgg
      661 gcgaatcttg cggttttgac gggattatca gggctttcca tgatgaagag aagacgctgc
      721 ggcgcctggc cggtatcgaa gaagagctgc tcaaaaatgc gcagagctcg agcgggaatc
      781 tgaaagaata catcgccgtg caccatgccg tcagaaaaac actccggcag acgcgaagcc
      841 tggcccggct gttacgaaaa aaaaggcagg cggcggaaaa cctacggaaa agcggagaaa
      901 aaataacaga aacagatgag cttgaagaat aagtcgcagg aaaaatttga ttgaatgaag
      961 gtattcgtat tatgtgggcg aaagtcgaaa tcactcattc cgacgaggat ttgatttatt
     1021 tgccgctctc tttgaccgga gaatatcggg agcatatcca aattaaattc ggggggctga
     1081 atgtcagagt aaaagtacgt tatgcggatg gtgcgggact tgagggcagc acgtatgacc
     1141 gcccgggagc gttgattttg tcggatcatc tcgtggaaaa gctaatgatt ccaagggccc
     1201 ctgtttatca gattaaaatc cgggagggca atcttcggat cggccctgtg attggctttc
     1261 ttctgggaag tcagacgcag cgatatagcc cggagcatat ggaaaaatac tccgaccggt
     1321 ttggaattta tcataaagtg ggcgggctga tctacgcatt ctcgccccgg tctatcgagt
     1381 ggaaaaaaaa tgcggcgttc gggctgtatt ataatattga aaaatccaaa tgggaatatg
     1441 gaaaatttcc tcttcctgaa acgatttacc ggagggattt tcattcagat cccgaggtga
     1501 tcgaaaagct gatccggtat acgcacggcc ggctgttcaa ttcctatcgt tttgacaaat
     1561 atgaacttta ccggtttgta ggccaaaacg aagaactcaa aaaatatctt cctccgactg
     1621 aaataatcga gagcgcgggg caggtatccg attttatcgg gcggtttcca tgctcaatca
     1681 tcaaaccggt cgacctttcc agaggacgtg gcattttggt tgttgaaacc aatgagggcg
     1741 gttacaagat taccgattac aggcaaaaag atccggtggt ggagcatctt gacggcgatt
     1801 ccatgctaaa tgcttttttt gaacgcaata ccgaattgtt ttcccagcat atcatccaga
     1861 aatatcttcc cttggcatcc atcggaagtt ctatttttga tgtcagggtt gtcatgcaaa
     1921 aaagggtgaa tcattcctgg ggatgctccg gaattgaatg cagggtgtcc aatgacgact
     1981 gcctgctgac caatatttcc aaaggaggat atgcgcttac tctaaaacat gcgcttcata
     2041 aatcgtttcc cgataacggc cgacagattc cgaaaaaaaa ttcttgacct ttgtaggaaa
     2101 atctgcgttt atctggatac gtcgggtcac cattacgcgg agcttggaat tgacattgcc
     2161 atcgacaggg atcaaaatct ctggctgatt gaggcaaacg tattcccgag tttcaaaggg
     2221 tttaaaaaga ttgacaggga aacctatctc agaatccggt attccccaat gatctatgcc
     2281 agatcactga ccggcttcga aaacagcgga ggagaaaaac atgtgtgaga cggtaaaaat
     2341 ccgacagatg gaaggcggcc ggaatattct tgcgatgaat ccgtcgcaag cggcgtcgct
     2401 ccattttgag ttcggcaaaa cggccgaggt cacatttgga aacctcagtc gttccgctca
     2461 gctcagaatg aataaaaatg tgcctccaaa tgaagtctgg atgtccggaa ggcttttgag
     2521 cggcctgcgt attccggatt atccggttta tgaattacgc gccgaagcgt caagactgac
     2581 cgtcgggccc tgcatcggcc tgctgctgtg ttcaaaaagc tctcgtttta ccgatgaatg
     2641 gctcggcagg ctcggggttt atctttcgga ttatgatctt cttcacggag cggttctggc
     2701 tttcgcgctc gatcagatcg acacggagaa gaagctgatc cggggctact gttaccggcc
     2761 ttccgatcaa agctgggtga agggtgttta tccttatccc tgcgcgatct atcggcggat
     2821 cggtctgagt gaccggtgga agcggcatca gccaataata ttttatgaaa cttactcata
     2881 cggcgttctg acaagttcat cccgagttgc gaaacgccaa acaccgggct tctgattcaa
     2941 aatgacaatt ccgtagaatt ttttttcaaa ttcccgcttt tttttcattt ttaattctat
     3001 gaaacaaaac gcaaatctga tatgatagga acaacttatt ttcaggcgaa aataatcggg
     3061 gccatatcga cccctaaatt attgaaaagc aggtagatta atgacagcac ttttattaac
     3121 ttttttctgc atagcggtta ttctggtcat gcttgtcatc tttaaaaaac ccgccgatat
     3181 ctccgggata acggggctca tcgtaatttc cctcattgcg atgctgttct ttcatacctc
     3241 actggaggta attcttcggt cggctgccgc aggttttgtc aaatcctttt ccgtctcgct
     3301 gattgtggcc acttcgcttc tgcagagggc cctgattgaa aaaagcggag cgttaaaaag
     3361 gattattatt ttcatcaaaa ccctctctcc ggaaaaccgg gcggctcaga tcatgctgat
     3421 caatatcgga ttcggtacgc tgatggtcgc ggtgggcgca acccccgtct ctcttctgcc
     3481 tccgattctt ctggcaatgg gatactcgac ctatgtggcc atcgcccttc cttccatcgg
     3541 ttacgattcg ctctgcacct atgctttgct tggcgcaccg atagtcgttt tcgttgatat
     3601 ggcaaacggt tttctgaaaa attcgggaat acatacggaa atcacacttt cacaggtcgg
     3661 acagattttt gcttat
//
LOCUS       DATALY010000313         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_583079,
            whole genome shotgun sequence.
ACCESSION   DATALY010000313 DATALY010000000
VERSION     DATALY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583079"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /gene="alaS"
                     /locus_tag="VHR42_08505"
     CDS             complement(<1..1304)
                     /gene="alaS"
                     /locus_tag="VHR42_08505"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX3027246.1"
                     /translation="MEWTGLNELREKYLAFFEGKDHLRLPSFPLVPQNDNSLLLINSG
                     MAPMKKYFLGQLTPPRKRVTTCQKCIRTPDIERVGKTSRHGTFFEMLGNFSFGDYFKH
                     EATAWGWEFITKVLNLPVDRIWVSIYQDDDEAFDIWTKEVGVDPARIVRLGKEDNFWE
                     HGSGPCGPCSELYFDRGEKYGCGKPTCGVGCDCDRYVEFWNIVFSQFDSDGNGTYTPL
                     EHPNIDTGMGLERLACIMQGVDNLFEVDTIANIMKHVSRIAGVKYGEGHKTDVSLRVI
                     TDHIRSTVFMVADGVIPSNEGRGYVLRRLLRRGARHGRLLGIQGPFLADVCDTVIAEN
                     EKAYPELVEKKDYIKRVIAMEEARFSDTIDQGLQLLNNQIEKLQQSGLSVLSGDEAFR
                     LYDGAAPILINLRKDFVVNRDFTGDHFSKVDKAACRGFFASG"
     gene            complement(1515..>1887)
                     /locus_tag="VHR42_08510"
     CDS             complement(1515..>1887)
                     /locus_tag="VHR42_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3027247.1"
                     /translation="PDRVFSESIANTIKKFMIYTVDSGTGTPAKPTYGGAGGKTATAE
                     TGWIQNGKTINQAWFAGFYPAESPRYAIVAMLENGVAGGADAGPVFKYIADSLAPSLG
                     YSAPSSSSDSDQTRTKKAAGE"
BASE COUNT          474 a          521 c          439 g          453 t
ORIGIN      
        1 taaccggatg caaaaaatcc ccgacaggcc gctttatcca ctttggagaa gtggtctccg
       61 gtaaaatcgc gattgacgac aaagtccttg cgaaggttga tgagaatcgg cgcagcgcca
      121 tcataaagac ggaaagcctc gtcgccggac agaacggaca atccgctctg ctgtagcttc
      181 tcgatctgat tattcaaaag ctggagaccc tgatcgatgg tgtcgctgaa tcgtgcttct
      241 tccatcgcga tcactcgctt gatataatcc tttttctcaa caagctccgg gtatgctttc
      301 tcattttcgg caataacggt atcgcagaca tcagcaagga aaggcccctg aataccaaga
      361 agtctgccgt ggcgagcgcc tctgcggagc agccgccgaa gcacatatcc ccggccttcg
      421 ttggaaggaa tgacgccgtc ggccaccata aagaccgtgc tacggatatg gtcggtgatg
      481 acgcgcagtg aaacatccgt tttatgacct tccccgtatt tcacaccggc aatccggctg
      541 acatgcttca tgatattcgc gatcgtatcg acttcaaaga ggttgtcaac gccctgcatg
      601 atgcaggcga gcctttcaag gcccattccg gtgtcgatat tcggatgctc gagaggagta
      661 taagtgccgt ttccgtcgct gtcaaactga gaaaaaacaa tattccagaa ttccacataa
      721 cggtcacagt cgcaccctac gccgcaggta ggtttgccgc atccgtactt ttcaccgcgg
      781 tcaaagtaca gctccgaaca cgggccgcac gggccggaac catgttccca gaagttatct
      841 tccttgccca ggcggacaat acgagcgggg tccaccccga cttctttggt ccatatatca
      901 aaagcttcgt cgtcatcctg atagatggat acccagatcc ggtccacagg aagattcagg
      961 actttcgtaa taaactccca gccccaggcc gtcgcttcat gtttaaaata atcgccgaaa
     1021 gaaaaattgc ccagcatttc aaaaaacgta ccgtgacgag acgtcttgcc aacccgctcg
     1081 atatccggag tgcggataca tttctggcag gtggttaccc tcttgcgcgg cggcgtgagc
     1141 tggccaagaa aatatttctt catcggagcc atgccggaat tgatcagcag aaggctgttg
     1201 tcattctgag gcacaagcgg aaaactcgga agtctcagat gatccttgcc ttcaaaaaaa
     1261 gcaagatatt tttcccttaa ttcgtttaac ccagtccatt ccataccttt tggagtcctc
     1321 cctaacggat ttctgtcaat gaaaatccgt tcctcagcca accggcccgg gaacggaatg
     1381 catttgcata tccataatcc aataaagtac cggcgaaaag cagaattacc ccctcaccgg
     1441 tcttttagac acaatagcat tatatcacag catttttgac aaattcaagt gaaattgcta
     1501 aaaaccgacg aagtttattc ccctgctgcc tttttagtcc gagtttggtc agaatcggat
     1561 gacgacgacg gcgcgctgta accaagtgaa ggcgccagac tgtcggcaat atatttaaac
     1621 accggccccg cgtctgcacc gcccgccact ccgttttcca acatggcgac aatggcgtaa
     1681 cgcggtgact cggccggata aaaaccggca aaccatgcct gattgatggt tttcccgttt
     1741 tgaatccatc cggtctcagc cgtcgcagtt tttcctccgg cccctccgta cgttggtttg
     1801 gccggagttc ctgttccgga atcaaccgta taaatcataa acttcttgat ggtgttggca
     1861 atgctctcgc tgaagactct gtcgggc
//
LOCUS       DATALY010000314         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_842005,
            whole genome shotgun sequence.
ACCESSION   DATALY010000314 DATALY010000000
VERSION     DATALY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842005"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..706
                     /locus_tag="VHR42_08515"
     CDS             368..706
                     /locus_tag="VHR42_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027248.1"
                     /translation="MLALLMLAAILVLGIILILRLIPKMQYRVLSIITLVLTVAFLAI
                     FTNAGRSMAKTLGIPMGADQQYAGQNFAAARGQNKTKRSGRTSSQAPAKGNTTAVFPD
                     GSQTVLRGTV"
     gene            808..1212
                     /locus_tag="VHR42_08520"
                     /pseudo
     CDS             808..1212
                     /locus_tag="VHR42_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1539..1763)
                     /locus_tag="VHR42_08525"
     CDS             complement(1539..1763)
                     /locus_tag="VHR42_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027249.1"
                     /translation="MNGDMEEYLICNTTREQRIKIIHDALSCNGVSCEECSACDAYGA
                     VSPLKMYQPYIDGKKEIREINHEYAANYMK"
     gene            complement(1907..2044)
                     /locus_tag="VHR42_08530"
     CDS             complement(1907..2044)
                     /locus_tag="VHR42_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027250.1"
                     /translation="MGVIYELFKLPKTDRKKFLDEVLRQETVGKLKAQENEGLRSKAD
                     K"
     gene            complement(2215..>2573)
                     /locus_tag="VHR42_08535"
     CDS             complement(2215..>2573)
                     /locus_tag="VHR42_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027251.1"
                     /translation="KVAGIVRNSTQSSASCRNSRSGGRLFRADRPTFARCVICRSVPS
                     GFCVLWYGEVIGMAQCCPGDDLVLLAALISIAVARGMGTEDLNILANLYSAIGSNLAT
                     IAAKRQACEDQKDSQV"
BASE COUNT          671 a          639 c          625 g          638 t
ORIGIN      
        1 taaaacattc gacgtaccgg tgagaacaga ggattttaca atacaagagt ttggcgtgag
       61 aggggcagaa acaaaaaaaa taattctact aactgttgaa tcgctatgct tttcaacgtt
      121 atcaactgca ttttcaacat cttgttgttg acaacctgaa ttcagctaaa tcgtgaatgc
      181 ctgttttcgg gtatattcca gctctgcaaa agtacgcagg gcaaagcaga gacagtactg
      241 ggcatccggc cgacgccggg tgctttttca ttttgcattc tcaaaactgt aaagggtgat
      301 tcaaactttt gcggtataag cggatagaaa gaaaacaaca atctgatctg ctgaaaggaa
      361 tgattctatg ttagcacttt tgatgctggc ggcaatcctc gttctgggca ttatcctcat
      421 cctgcggttg attccgaaaa tgcagtaccg cgttctgtcg attatcacgc tcgtgcttac
      481 cgtcgctttt ctcgccatct ttacaaacgc aggaaggtcc atggcaaaga ccctcggcat
      541 cccaatgggc gctgaccagc aatatgcggg gcagaatttc gcggcagcca gagggcagaa
      601 caagacaaaa cgttccggcc gcacttcttc acaggctccg gcgaaaggaa acacaactgc
      661 ggtgtttccg gacggatctc agactgtttt gagaggcaca gtctgagcat ccgcgagaaa
      721 ataattctcc aaaaggcaca tatccggaaa attctcatag aatggcataa cagaatgaaa
      781 ggagaatgcc cggatatgcc aaacgcagaa ctgaaagact acggccctgc accgatcgta
      841 gtcaacattg aaggcgcaac caaggtaaac gatacattcc gcacagccct gtggactgga
      901 aagtacctgc agataaccct gatgagcatt aagcccggcg aagatatcgg tcctgaaatg
      961 caccctggca ccgaccagtt tatccgtgtg gagcagggcg aagggcttgc aaaactcggc
     1021 ggccggaaag acgacctgag cttccagaaa aaaatcgggc cgagcgatgc agtcgtggtt
     1081 cccgccggga cgtggcataa tattgtcaac atcgggaaga tgccgctcaa ggtttattcc
     1141 atctatgccc cgccgaaaca tccgaaggga acggttcacc agacaaaggc cgatgcgcag
     1201 gcagctaaat aaaataaagt ttacccggcc tgttttgatg ggccgggtaa attattcttt
     1261 ttagtttttt gtatgaaggg actcgaatca tgtcgaattt tcgtatataa tattattgag
     1321 gtgaatattt atgactgccg aggaactcaa aaagctggac aggcagatcc gcgacattcc
     1381 atatccgtgc agagagaatt ttcctgtgct taaaaaaatc ctccgagatt tgtccgaccg
     1441 gcaggaactg cctgtgtcgt ccatcctaca gcagtatttc gcgtggaaat ggaccaaatg
     1501 atttgatgat tcgccggccg aaaggccggc gagctgtttt atttcatata attcgcggcg
     1561 tactcgtggt tgatttcgcg aatctctttc tttccgtcaa tatacggctg atacattttc
     1621 aaagggctga ctgcgccgta tgcgtcacag gcggagcatt cctcgcagct gactccattg
     1681 cagctcagcg cgtcgtggat gatttttatc cgttgttccc gcgtcgtgtt gcagatcaga
     1741 tattcttcca tatctccgtt cattatgatg cctcccgcag caaaaattgt tcggccggtc
     1801 ttataagatt atcgtagtcc gaaaagtgca gactgtaaag gcgtttccgg caaaaacggg
     1861 aaacgggcaa aaaaataccc gcggctttct gccacggttt cgtcgtctac ttgtccgctt
     1921 tgctgcgaag accttcgttt tcctgcgctt tcagcttccc aactgtttcc tgccgcagga
     1981 cttcatcgag aaatttttta cggtccgttt tcgggagctt gaaaagctca taaatgacac
     2041 ccataggtct tcctcctttc tgatgctatc gtactgcggg ctttgggcgg ctgcaagtca
     2101 agccatgtaa atctgacgta atatttcggt caggcgggtt cttcacactt tccaccataa
     2161 attcaacata tggtgccata aacagcttga ttacccatat gttccattac ggactcagac
     2221 ctgagaatct ttctgatctt cacaggcctg ccgttttgcc gctatggtcg caagattgct
     2281 gccgattgcg ctgtacagat tcgcaagaat attgaggtct tccgttccca tgcctctggc
     2341 aacagcaatg gaaatgagag ccgccagcag gacgaggtcg tcaccgggac agcactgcgc
     2401 catgccgatc acctcaccgt accataatac gcagaagcct gacggaacag acctgcaaat
     2461 cacacagcgg gcgaatgttg ggcggtctgc acgaaaaagc ctcccgccgg aacggctgtt
     2521 ccggcaggag gctgacgatt gggtgctgtt acgaacaatc ccggcaacct tcc
//
LOCUS       DATALY010000315        13821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_154805,
            whole genome shotgun sequence.
ACCESSION   DATALY010000315 DATALY010000000
VERSION     DATALY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13821
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154805"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..678
                     /gene="hpt"
                     /locus_tag="VHR42_08540"
     CDS             139..678
                     /gene="hpt"
                     /locus_tag="VHR42_08540"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX3027252.1"
                     /translation="MLNDIEKVLITEEDIAKKVAEIGQKISGDYQGKNLLMVSILKGS
                     VVFMADLLRQVKIPARIDFMSVSSYGAGVKTSGVVKIIKDLDIPLEGYDVLIIEDILD
                     SGLTLHYIIDILKSRNPRSIKVCTLLDKPESRKAYVKSDYSGFVIPDEFVVGYGLDYD
                     EKYRNLPFIGILKPEVYAK"
     gene            731..2587
                     /gene="ftsH"
                     /locus_tag="VHR42_08545"
     CDS             731..2587
                     /gene="ftsH"
                     /locus_tag="VHR42_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX3027253.1"
                     /translation="MKNFRGIGLYIVILLAIILGLSLFSMNQQTEKKLMYSDVINYFE
                     DGKVSQYSLDLGNGDLVLTLRDKKVVKYTVPNVDLFIDQVKPLVNDYNKKNVDTRIAY
                     DYIQAWQAPWWFSFLPYLLLIAVMVFFWIYFLNQAGGGAGGGKVMSFGKAKVKQVSDE
                     KKKTTFADVAGADEEKEELQEIVEFLKNPHKFNEIGARIPKGVLLVGPPGTGKTLLAK
                     AVSGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSSPCIIFIDEIDAVGRHRG
                     AGLGGGHDEREQTLNQLLVEMDGFGANEGVIIIAATNRPDILDPALLRPGRFDRQVFV
                     GVPDIKGREEILRVHSRGKPLAPDVELAEIAKTTVGFTGADIENLLNEAALLAAKKNK
                     RVISMKEIEESAMKVIAGPEKKTRIISEKEKRLTAFHEAGHAVVTKFLPSQNPVQQIS
                     IIPRGRAGGFTLTPPVEDKYYMSKNEMFEEICTLLGGRVAESLELEDISTGASNDIER
                     ATDVAHKMVTKYGMSDKLGPIVFGSGHDEVFLGRDFAQARNYSESIASQIDSEIKSII
                     DSAYEKTKTILQGKSDKLKEVAAYLVKHEKMSGTEFEKIFEDSPELPEAPMA"
     gene            2611..3366
                     /locus_tag="VHR42_08550"
     CDS             2611..3366
                     /locus_tag="VHR42_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027254.1"
                     /translation="MKRPFLSRALYGLVLIVIGVGILGHMANFWDLGYLARGWWALLL
                     IVPGLAGIISSGLQFWNCFLVIFGGWLYIRAHGWLGNNSFPWLLGIALIAFGIWTITG
                     GGRNGKFEEDYRSNTQNGTNGKDGRHSWRYSSSEHVNQDSDDFPEYSSVFSGYRVANK
                     CKAFRGGKASSVFGRLTLDLREIEITGRAVLEVASVFGNLEILLPPGVPVKSNVVPVF
                     GIYRNNSAEPHSGPENSGLEIKGSAVFGTIDIY"
     gene            3563..5548
                     /locus_tag="VHR42_08555"
     CDS             3563..5548
                     /locus_tag="VHR42_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX3027255.1"
                     /translation="MARGKNEVYGNESISSLKGADRVRKRPGVIFGSDGLEGCEHSIF
                     EILSNSIDEAREGYGSRIVITRYSDGSVEVEDFGRGIPVDYNSNEGKYNWELVFCELY
                     AGGKYNTREGESYEFSLGLNGLGLCSTQYASEYMDVEIKRDGHRYKIHFEHGENVGGL
                     EKHRISDKTTGTRIRWKPDLSVFTDIDIPIDYYLDTLKRQAVVNAGLVFELKNQTAEG
                     FETFEFCYQEGIADYVRELAGGEFITPVQYWQTERQGRDREDKPEYRVKISAAFCFHN
                     KANKIEYYHNSSWLEHGGSPEKAVRSSFVSQIDAYLKQNNKYLKNESKISFADIEDCL
                     LLITSSFSTQTSYENQTKKSINNKFIQEAMTDFLRRQLEIYFIENPADAEKVAEQVLV
                     NKRSRENAEKTRLNLKKKLSGSLDLSNRVQKFVDCRSRDTDRREIYIVEGDSALGACK
                     LGRDAEFQAIIPVRGKILNCLKAEYDRIFKNEIITDIIKVLGCGVEVKSKANKELHSF
                     ELTQLRWNKIIICTDADVDGFQIRTLILTMLYRLVPTLIDKGFVYIAESPLYEITTKE
                     RTYFAYTDREKSEVVEKIGDQKYAIQRSKGLGENEPDMMWETTMNPVTRRLIRVSPED
                     AAQTNYVFDLLLGDNLAGRKQFIADKGSLYLDLADVS"
     gene            5586..7820
                     /locus_tag="VHR42_08560"
     CDS             5586..7820
                     /locus_tag="VHR42_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HEX3027256.1"
                     /translation="MARKNDQYPKRKKSLKSEIEGAGTVQDQPITRTLETNYMPYAMS
                     VIVSRAIPEIDGFKPSHRKLLYTMYKMGLLTGHRTKSANVVGQTMRLNPHGEASIYDT
                     LVRLSRGYEALLHSYVDSKGNFGKFYSRDMAYAASRYTEVKLDALCSELFREIDKETV
                     DFIDNYDNTTKEPVLLPATFPSILVNSNTGIAVGMASSICSFNLAEACETTIRLIKNP
                     AHNIESTLKAPDFSTGGLILYDGTALDTIYKTGRGSLKVRARYGFDKAGNCIEITEIP
                     PTTTVEAIIDKVIELVKSNKIREISDIRDETDLSGLKLTIDLKRGAEPEKLMAKLFRF
                     TPLQDSFACNFNILVGGTPKVMGIAEIVSEWHAFRSECIRRRVYYDLARCKDKLHLLL
                     GLGKILLDIDKAVTIIRKTEEESEVIPNLMIGFGIDEIQAEYVADIKLRHLNREYILK
                     RTQETDQLEQEIERLSSLLADKKKIDEVMISELKDVIKKYAKPRKSLIIYDDSGENEL
                     IEEPAEDYPVHLFITADGYFKKITPQSLRVSGEQKLKEGDSVRSTQESSNRKDLLFFT
                     DRCQVYKSKVSDFDDMKASQLGEFIPAKLGFDEGENVVYMAVTTDYSGFMLFFFQNGK
                     AAKVEMSVYQTKTNRKKLVGAFNDKSPLSGIDQRSEDGEYVLSSSSGRILLIHTGAIP
                     AKTTRATQGVQAMKLKPGQFVTGARVHTEGMFKSPHRYRTKSLPAVGQRPSPGDTSGE
                     QLSF"
     gene            7970..8563
                     /locus_tag="VHR42_08565"
     CDS             7970..8563
                     /locus_tag="VHR42_08565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3027257.1"
                     /translation="MTKVYLVRHGETDWNRQGRIQGRENIPLNANGLRQALYCGLYLK
                     KFMPFAAVISSPLDRARKTAEVIAETLEMKREIIIMNDLIERDFGEISGLLPEERELR
                     LASGETLGIETQEDLRGRAMKALNTISTKFRGGGIIVVAHGAIINAMLAVISGGKIGT
                     RKTLLTNGGVSVVENSGERWSVELHDHIPALPFSDIG"
     gene            8693..8947
                     /gene="secG"
                     /locus_tag="VHR42_08570"
     CDS             8693..8947
                     /gene="secG"
                     /locus_tag="VHR42_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX3027258.1"
                     /translation="MSALQIIASIMLFISSVVLVVIVLMQHGRDAYLGGAIAGGAAES
                     FLGKNKARTIDSILSKYTKIIAVTFLVLTLVVNAIVYFIK"
     gene            9069..11177
                     /gene="rnr"
                     /locus_tag="VHR42_08575"
     CDS             9069..11177
                     /gene="rnr"
                     /locus_tag="VHR42_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX3027259.1"
                     /translation="MNRTLLKEQVLHLIDQQNYRPMMINELAKSLSVPVESWPEFFNM
                     LKQLEIEGAITVAKSKKVMKADRKNVQTANMRTVTGKGGYASLTDQSSDVFIDKKDLR
                     GAMPTDVITVRILKRSGRLPQAEVIKVVERKFIEFTGTLYKRGKHAYIIPDSGTKEKL
                     EVGPVDSREAKDRDKVLAKIKKYSGKGHTTPTAQIITSYGSSESAAACSLAVLDRYHV
                     RRDFPAVVSEQAKQASADLQIEPSRLDLRGQPIFTIDGATAKDLDDAVSVEKTGKGYR
                     LGVHIADVSHYVRAGSELDKEAFKRGTSVYYADKVIPMLPKELSNGICSLNPKEDRMT
                     FSALIELDNGGRILSYELKKSVINSRVKGVYDEINRLFGGSESPEIREKYREVLPELT
                     LMRRLYEILHAARLQRGAMDFETDESEIVIGAGGTAVDIKKRVRGDAEKMIEEFMLCA
                     NEVVATFAQSDAIPFVYRVHEEPDARKLEVLKAALKAAGISDRSIHSGLRPSDMAALL
                     KAIEGSPKAKALNDLVLRSMAKAKYSPECAGHFGLSLKNYCHFTSPIRRYPDLAIHRI
                     LGDCLKNGNDDKLKKRYGSFVEEASIHSSEREVAAMQLEWDCEAIYKAEYMTAHIGEQ
                     FIGTVTSVKSFGMYVTLENTVEGLVRVENLPGGWYDYDEASISLYCAQNGRRYTIGDE
                     VKIIVAKAEVATGRIDFEVL"
     gene            complement(11267..11434)
                     /locus_tag="VHR42_08580"
     CDS             complement(11267..11434)
                     /locus_tag="VHR42_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027260.1"
                     /translation="MSNQEIKTVVERYCPVIGKNVAVEISRTGQSKPSCLNSHDCETS
                     GGCKNKLFTGR"
     gene            11717..11986
                     /locus_tag="VHR42_08585"
     CDS             11717..11986
                     /locus_tag="VHR42_08585"
                     /inference="COORDINATES: protein motif:HMM:NF022165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HEX3027261.1"
                     /translation="MEGAIAGVIQMTLNRKPEVSPEQQLICELEAVKQNLDTVSERFE
                     YQSDPDLVEACIYEMQALTARYRYLTREARRLGLTKSALLSLRQI"
     gene            12154..12411
                     /locus_tag="VHR42_08590"
     CDS             12154..12411
                     /locus_tag="VHR42_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HEX3027262.1"
                     /translation="MKTYVYIVIASVICAFLIALLRSKRLVKGIALSSLGGLAALGAV
                     DLSGMLTGVTLAFNLWTFLTAAVLGLPGVVSMLLVKLMWGI"
     gene            complement(12466..12906)
                     /gene="mscL"
                     /locus_tag="VHR42_08595"
     CDS             complement(12466..12906)
                     /gene="mscL"
                     /locus_tag="VHR42_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX3027263.1"
                     /translation="MIYLNRIKSLISEFKTFAIKGNAVELAIGVIIGNAFNKIVTSLI
                     NFIITPLLSMFLGKTDIANLQISIPSRFNRRVPIGIKYGLFLQAVLDFLTVSLSIFVM
                     IKLVNKFHNKQDSEPKEEDLSSTDALLTEIRDLLIAQKDDGREK"
     gene            13191..>13821
                     /locus_tag="VHR42_08600"
     CDS             13191..>13821
                     /locus_tag="VHR42_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027264.1"
                     /translation="MRIISRTLSVLLMMLWDSAAVAGSVIISFPLRYGFDRVLPVKYT
                     EFLKEYIILSIIVVLICNTICKCYMNVWRHAGINEYARQMISVLLSAAIMVFIDFTRH
                     LGLGIELLIIIAMFEMLLMLGVRVSVRSFFFLHSRAMSMIRRKKMERVLVFGAGEAGT
                     NLVHKLESHPEENRLPICFVDDNDSKWGKKICGLPVVGGRDKLVKTIRDY"
BASE COUNT         3686 a         2965 c         3676 g         3494 t
ORIGIN      
        1 gagaccggat tccggttatt gccgacgagg aaggcgtttt atgggttcct cagttcgggc
       61 cggacgagag atgtggaatc aacaaagaga cccgaaaaat ttttgttgtt gaaattcaag
      121 ctttggaggt ataaactgtt gctgaatgat atagaaaagg ttttgataac tgaggaggat
      181 attgccaaaa aggttgccga aatcggccag aaaatttctg gggattatca gggaaagaac
      241 ctcttaatgg tcagtattct caaaggctcc gtcgtcttta tggcggattt gctccgtcag
      301 gtcaagattc ccgcgagaat tgattttatg tctgtttcaa gttatggcgc aggtgtaaaa
      361 acctccggag tggttaaaat tattaaagat cttgatattc cgctggaagg ctacgatgtc
      421 ctgattatcg aagatattct tgacagcggg ctgactctgc attatattat agatatttta
      481 aagtccagaa accccaggag tattaaagtc tgcacgctgc tcgataagcc cgaatcccgc
      541 aaagcctatg tgaaatccga ttattcagga tttgtgattc cggatgaatt tgtggtcggc
      601 tatgggctcg actatgacga gaaatatcgc aacctgccat ttatcggaat tttgaaacct
      661 gaggtatatg caaaataaag cagcaatgtt gatcgcatta gccatattgg catcagggag
      721 tgagttttaa atgaaaaatt ttcgaggaat cggattatac attgtgatac ttctggcaat
      781 cattctcggg ctaagtctgt tcagtatgaa ccagcagacc gagaaaaaac tgatgtattc
      841 cgatgtcata aactatttcg aagacggaaa agtgtcacag tattcgctgg atcttggcaa
      901 cggcgatctt gtgctgacac tgcgcgacaa aaaagttgtc aaatacacgg ttcccaacgt
      961 cgatcttttc atcgaccagg taaagcccct tgtcaacgat tacaacaaga aaaatgtgga
     1021 tacccggatt gcctatgatt atattcaggc atggcaggca ccgtggtggt tcagctttct
     1081 cccttatctg ctgctgattg ccgtaatggt ctttttctgg atctattttc tcaaccaggc
     1141 cggaggaggc gcaggcggag gtaaggtgat gtccttcggc aaggcgaagg tcaaacaggt
     1201 ctcggatgag aagaagaaaa ccacttttgc cgatgtagcc ggcgcggatg aagaaaagga
     1261 agagcttcag gaaatcgttg aatttttgaa aaatccgcac aagtttaacg agatcggcgc
     1321 gcgtattccc aagggcgttc ttcttgtggg ccctcccggt accggtaaaa cgcttcttgc
     1381 aaaagcagtt tcgggtgaag ccggcgtgcc gttcttctcc atatcaggtt ctgattttgt
     1441 cgaaatgttt gtcggcgtgg gcgcttcgag agtccgcgat ctgtttgagc aggcaaaaaa
     1501 gagctcaccc tgtattatct ttattgatga aattgacgcg gtcggccgcc atcgcggcgc
     1561 cggtttgggc ggcggccacg atgaacgtga gcagacgctg aaccagctcc tggtggagat
     1621 ggatggcttc ggagcaaatg aaggcgtaat tattatcgcg gctaccaacc ggcccgatat
     1681 cctcgacccg gctctgcttc gccccggccg atttgaccgt caggtgtttg tgggtgtgcc
     1741 ggatatcaag ggcagggagg aaattctccg ggttcattcc cgtgggaagc cgttggcccc
     1801 tgatgttgaa cttgccgaaa ttgccaaaac cacagtcggc tttacgggcg cggatattga
     1861 aaatctctta aacgaagcag cgctgcttgc cgccaaaaag aacaaacgcg ttatttcgat
     1921 gaaagagatc gaggagtccg cgatgaaggt catcgccggg ccggaaaaga aaacaagaat
     1981 tatatccgaa aaagagaaaa gactgactgc tttccacgag gcggggcatg cggtggtaac
     2041 caaattcctg ccctcccaga acccggttca gcagatctcg atcattcccc gcgggcgggc
     2101 cggcggattt acactgacgc ccccggtgga agataaatat tatatgtcca aaaatgaaat
     2161 gtttgaagag atttgtacgc ttctcggcgg ccgtgtcgca gaaagtctcg aacttgagga
     2221 tatttccacc ggcgcttcca atgatattga gcgtgcaacc gatgtcgcgc ataaaatggt
     2281 taccaaatac ggtatgagcg ataagctggg cccgattgtc ttcggctcgg gtcatgatga
     2341 ggttttcctt ggccgggatt ttgcgcaggc caggaactat tcggaaagta tcgcttctca
     2401 gatcgattcg gaaattaaaa gtattattga ttctgcgtat gaaaaaacaa aaacgattct
     2461 ccagggtaaa tctgacaagc tcaaagaggt tgcggcatat ttggtgaaac atgaaaaaat
     2521 gtccggcaca gagtttgaaa agatttttga ggattctccg gaacttccgg aagctccgat
     2581 ggcctgagtg gaatctgtaa ggggtattac atgaaaagac ctttcctgtc ccgggcgctt
     2641 tatggcctgg tgctgattgt gatcggcgtc ggaattcttg gccatatggc gaatttttgg
     2701 gatctgggtt atctggcacg cggctggtgg gcgctgctgc ttattgtccc cggtctcgca
     2761 ggtatcatct cctccgggct tcagttctgg aactgctttc tggtgatatt tggcggatgg
     2821 ctttatatca gagcgcatgg ttggcttgga aacaacagtt ttccgtggct tctcggaata
     2881 gcgctgattg cattcggaat ctggacgatt acgggcggag gccggaatgg aaagtttgaa
     2941 gaagactacc gctctaacac gcagaatgga acgaacggta aggatggccg gcacagctgg
     3001 aggtattctt catcggagca tgtgaatcag gattcggacg attttcctga atattcaagt
     3061 gttttttccg gttatcgggt tgccaataag tgcaaagcct tccggggagg aaaagccagc
     3121 agcgtgtttg gaaggctgac gcttgatctg cgggagattg agatcaccgg gcgtgctgtc
     3181 ctggaagtgg cgtccgtttt cggtaatctg gagatccttc tgccgcccgg agtcccggtc
     3241 aaatcgaatg tcgtcccggt ttttggaatc taccgcaaca attcggcgga accgcattcc
     3301 gggccagaga atagtggtct tgaaatcaaa ggatcagcgg tatttggcac gattgacatt
     3361 tactaattca aaaagccctt aaaacgggag cgcgcaagcg tctcccgttt tattgtgagg
     3421 aatatgggcc tgccgggcac gacggagttt gcccggcggg attatacgca ttatcttgac
     3481 aaaaaatgca cctgtgggct acagtataga atagacttta ggggcaaatt actgaaatgg
     3541 caaatgaggg agggactcaa aaatggcacg aggcaaaaat gaagtttatg gaaatgaaag
     3601 catctcgtcg ttaaagggtg ccgacagggt ccgaaaacgt ccgggggtta tttttggttc
     3661 cgatggtctg gaaggctgcg agcattctat ttttgaaata ctgtccaatt caattgatga
     3721 agccagagaa ggctacggca gccggattgt cataacacga tattccgacg gttccgtgga
     3781 ggttgaagat ttcggccggg gaattccggt tgactataac agtaatgagg ggaaatacaa
     3841 ctgggagctg gtcttttgtg agctttatgc gggaggaaaa tataataccc gcgaggggga
     3901 aagctatgag ttttcccttg gactcaacgg tctcggcctt tgttcgacgc aatacgcttc
     3961 ggaatatatg gatgttgaga tcaaacgcga cggtcatcgc tataagatac atttcgagca
     4021 tggcgaaaat gtcggaggcc tggaaaagca caggatttcg gataaaacca ccgggacccg
     4081 aatccgatgg aagcccgacc tgtcggtttt caccgatatt gatattccga ttgattatta
     4141 tctcgacact ctgaaaaggc aggccgtagt caacgcgggc ctggtatttg aattgaaaaa
     4201 ccaaacggcg gaaggttttg agacctttga attctgttat caggaaggaa tcgccgatta
     4261 tgtccgggaa cttgccggag gggaatttat cacgcccgtg cagtactggc agaccgagcg
     4321 tcagggacgg gatcgggagg acaagccgga gtaccgggtt aaaatttcgg ctgcattctg
     4381 ttttcataac aaagccaata agattgagta ttatcataat tcgagctggc ttgagcatgg
     4441 cggttcgccc gaaaaggccg tgaggagttc atttgtttcc caaatcgatg cctaccttaa
     4501 gcagaataat aaatacctta aaaatgaaag caaaatctct tttgccgata ttgaggactg
     4561 cctcctgttg attacctcgt cattttccac ccagacttcc tatgagaatc agacgaagaa
     4621 atccatcaac aataaattca ttcaggaagc catgacggat tttctccgcc ggcagctgga
     4681 gatatacttc atagagaacc ccgcggatgc tgaaaaagtc gccgaacagg ttctggttaa
     4741 taaacgcagc cgcgagaatg ccgagaaaac ccggctaaat ctgaaaaaaa agctttccgg
     4801 ttcgctggat ctttccaatc gtgttcaaaa gtttgtggat tgccgcagcc gcgataccga
     4861 ccggcgggaa atttacatcg tggagggcga ttccgccctt ggagcctgca aacttggccg
     4921 ggacgccgag tttcaggcaa tcataccggt tcggggaaaa attctcaatt gtctcaaggc
     4981 ggaatatgac aggattttta aaaatgagat tattaccgac attatcaagg ttttgggctg
     5041 cggggtggaa gtgaaatcaa aagccaacaa ggagcttcat tcgtttgaac tgacccagct
     5101 gagatggaac aagattatca tctgcaccga tgccgacgtt gacggatttc agatccgtac
     5161 gctgatactg acgatgcttt accgccttgt gccgacgctg attgataagg gctttgtcta
     5221 tattgccgaa tcgccccttt atgagatcac aacaaaggaa cgtacctatt ttgcctacac
     5281 cgaccgggaa aaatccgagg tggttgaaaa aatcggagat cagaaatatg ctattcagcg
     5341 ttccaaaggc ctcggcgaaa atgagccgga tatgatgtgg gaaactacga tgaatccggt
     5401 aacaaggcgg ctcatccggg tgtcgccgga agacgccgcg cagacgaatt atgtatttga
     5461 cctgcttctt ggcgacaatc ttgcagggcg caaacagttt attgctgata aaggctccct
     5521 gtatctcgat ctggccgacg tatcctaaag agcgccggat agaaacgtaa atcggaagtg
     5581 atgaaatggc ccgtaagaac gaccagtatc ccaaacgaaa aaagagttta aagtctgaga
     5641 tcgaaggcgc cggaaccgtt caggatcagc cgatcacccg gacgctggag accaattaca
     5701 tgccttatgc catgagcgtt attgtgtcgc gggcaattcc cgaaatcgac gggtttaagc
     5761 cctctcaccg gaagctgctt tataccatgt ataaaatggg cctgctgacc ggtcatcgaa
     5821 ccaaatcggc caatgttgtg ggccagacga tgaggctgaa tccccacgga gaggcgtcga
     5881 tttatgatac gctcgtccgg ctctcgcggg gctatgaggc gctgctgcat tcttatgtcg
     5941 attccaaagg aaattttggt aagttttatt cgagggacat ggcctatgcg gcttcccgtt
     6001 acaccgaggt aaagctcgac gcgttatgtt ctgagctgtt tcgcgagatt gacaaggaaa
     6061 cggtcgattt tatcgacaac tatgacaata ccacgaaaga gccggtgctg ctgccggcca
     6121 cctttccgtc gattctggtg aactccaata ccggaatcgc ggttggtatg gcaagttcca
     6181 tctgttcctt caatcttgcc gaagcctgcg aaacgacaat ccgtctgatc aaaaatccgg
     6241 cgcacaacat tgaatcgacg ctgaaagctc ccgacttttc aaccggcggg ctgattcttt
     6301 atgacggaac agcgctcgat acgatttata aaacaggccg gggcagcctg aaagtccggg
     6361 ccagatacgg ttttgacaag gccggcaatt gcatcgaaat cacagagatt cctccaacca
     6421 ctacggtgga ggccataatc gataaggtaa ttgaactggt caaatccaat aagatccgcg
     6481 aaatctcaga catcagggat gaaaccgatt tgtcgggttt aaaacttacg attgacctca
     6541 aacggggtgc ggagccggaa aagctcatgg ctaaactctt ccggtttacg ccgctgcagg
     6601 attcttttgc ctgcaacttc aacatacttg tcggcgggac tcctaaagtg atggggatcg
     6661 cggaaatcgt ttccgaatgg cacgcgttcc gcagcgaatg catccggcgc agggtttatt
     6721 acgacctggc cagatgcaag gataaactgc atcttctact cgggctcgga aagatacttc
     6781 tcgatattga caaagcggtt accattatcc gaaagacgga ggaggagagc gaagtgatcc
     6841 ccaacctcat gatcggtttt ggaatcgacg aaatacaggc tgaatatgtg gcagatatta
     6901 agctgcggca tctgaaccgg gaatatattc tcaaacgtac acaggaaacc gatcagcttg
     6961 agcaggagat cgaacggttg tcctcattgc tggcggataa gaaaaaaatt gacgaggtca
     7021 tgatttccga gctgaaggat gtcatcaaaa aatatgcaaa gccgcggaag agtctgatta
     7081 tttatgacga cagcggagaa aatgagctga ttgaagaacc ggcggaggat tatcctgttc
     7141 atttgtttat cacggctgac ggttatttca aaaaaatcac gccacagtct cttcgcgtgt
     7201 ccggtgagca aaagctcaag gaaggggatt ctgtccgcag cacgcaggaa tcctcgaacc
     7261 gtaaggacct tttgtttttc accgatcgtt gtcaggttta taaatccaaa gtgtcggatt
     7321 ttgacgatat gaaagcaagc cagcttgggg aatttattcc cgcaaagctg ggattcgacg
     7381 agggcgagaa tgtcgtttat atggcggtta ccaccgatta cagcggattt atgcttttct
     7441 tctttcaaaa tggaaaagcc gcaaaggtgg aaatgtctgt ttatcagaca aaaaccaatc
     7501 ggaaaaagct ggtgggtgct ttcaatgaca agtccccgct ttctggaatt gatcagaggt
     7561 cggaagatgg cgaatatgta ctgagttcct cttccggcag aattctgctg attcacaccg
     7621 gagcgattcc cgccaaaaca acccgtgcaa cacagggtgt gcaggcaatg aagcttaagc
     7681 cgggccagtt tgtcacaggg gcccgggttc ataccgaagg aatgtttaaa tccccgcacc
     7741 gttaccggac caaatccctg cccgcggtgg gccagcgtcc ttcgccgggg gacacttcgg
     7801 gcgaacaact gagtttttaa taaaaaaccc gctccagcca aaaggtggaa cgggtgtggc
     7861 tgccggaact gcaaacgcaa agaaccatat tccggctgcc gttattagaa tatccgtttt
     7921 ttcattgatt ataaccggaa cgggaatttt ttgaataaag gattgtctta tgaccaaagt
     7981 atatcttgta aggcacgggg aaacggactg gaacagacag ggccgaattc agggccggga
     8041 gaatattccg ctgaatgcca acggattgcg gcaggcgctg tactgtggcc tgtatcttaa
     8101 gaaattcatg ccgtttgccg ctgtaatatc cagcccgttg gacagagccc gcaaaacagc
     8161 ggaggttatc gccgaaacgc tggaaatgaa gcgggaaatt atcataatga atgatttgat
     8221 cgagcgggac ttcggtgaaa tttccggcct gttgccggag gagagagagt tgagactcgc
     8281 atccggggag acccttggaa ttgagactca ggaagactta aggggcaggg cgatgaaggc
     8341 gctcaatact atttcaacaa agtttcgcgg cggcgggatt atcgttgtag cccacggagc
     8401 aatcataaac gccatgctcg ccgtgatatc cggaggaaaa atcggaacgc gcaaaacatt
     8461 gcttaccaat gggggcgtat ccgtcgttga gaattccggt gaacgatggt cggtcgaact
     8521 gcatgaccac attccggcac tccctttctc tgatattgga tgatttcgca atcaggttct
     8581 tgcaattaaa aggaacctat gatatgataa agtatatgct gtatcttttg tgaatgttgg
     8641 attctattta tcgatattga tattattagc agagtagaaa ggtgtgttca cgatgagcgc
     8701 tcttcaaatt attgcgagca tcatgctgtt tatttcttcg gttgttttgg tggtgattgt
     8761 gctgatgcag cacggccgcg acgcctacct cgggggggca atcgccgggg gagcggcgga
     8821 gtcgttcctt ggaaaaaata aggccagaac catagacagt atcctttcaa aatatacaaa
     8881 aataattgcg gttactttcc ttgtcctcac tcttgtggtc aacgcgattg tttattttat
     8941 caagtagtct gcccaactca ccccgcttca ggcggggttt tattttttat ctgcaatttt
     9001 ggggtgacaa ggtgacatac taaggttgtg cgatccggcg gcagaattca taggataggg
     9061 tgattaaaat gaatcggacg ttgttaaaag aacaggtttt acatttgatt gatcagcaaa
     9121 attacaggcc gatgatgatt aacgaacttg caaagtcttt atctgttccg gtggagtcat
     9181 ggccggagtt ttttaatatg ctcaaacagc ttgaaatcga gggcgcaatt accgttgcta
     9241 aatccaaaaa ggtgatgaag gccgaccgga agaatgtcca gacggccaat atgcgcacgg
     9301 ttaccggaaa gggcggctat gcctctctga ccgaccaatc cagtgatgtg tttatagata
     9361 aaaaagattt aagaggcgcc atgcccacag acgtcattac ggttcgtatt ctcaaaagat
     9421 cgggtcgtct tcctcaggct gaggttatta aggtggttga gcggaagttt atcgagttta
     9481 ccggaacgct ttataaacgc ggcaagcacg cctatatcat tcccgacagc ggaacaaagg
     9541 aaaagctcga ggtcggcccc gtagattcgc gcgaagccaa agacagggac aaggtgcttg
     9601 ccaaaatcaa aaaatattcg ggaaagggtc ataccacccc aaccgcgcag attattacct
     9661 cctatggttc ttcggaaagc gcggcagcct gctctctggc agttttggac cggtatcatg
     9721 tgcgcaggga ttttcccgcc gtggtttcgg agcaggcgaa acaggcatcc gccgacctgc
     9781 aaattgaacc gtcccggctt gatttgagag gtcagccgat ctttacaata gacggtgcga
     9841 cggcaaagga ccttgacgat gcggtttcgg ttgaaaaaac cgggaaaggc tatcgtctcg
     9901 gcgttcatat tgcggacgtt tcccattatg tccgggcagg ctccgaactc gacaaagagg
     9961 cctttaaacg cggaacatcg gtctattatg ccgataaagt catcccgatg ctcccgaagg
    10021 aactttccaa cggaatctgt tcgctgaacc cgaaagagga taggatgacg ttctccgcgc
    10081 tgatcgagct ggataacggc ggaagaatac tttcttatga gcttaaaaaa agtgtgatca
    10141 attcaagggt caaaggtgtc tatgacgaaa tcaaccggtt atttggcggg agcgaaagcc
    10201 ctgagatcag ggaaaagtac cgtgaagtat taccggaact gacactcatg cgccggttat
    10261 atgagattct tcatgctgcc cgcctgcaga ggggggctat ggattttgaa acggacgaaa
    10321 gcgaaattgt gatcggcgcc ggcggaaccg ctgtggacat caaaaaacgg gtgcgcggcg
    10381 acgcggaaaa aatgattgag gaatttatgc tgtgtgcgaa tgaggtggtt gccacctttg
    10441 cgcaatcgga tgcaattccg tttgtgtacc gtgtccatga ggaaccggat gccagaaagc
    10501 ttgaagttct gaaggcggcg ctgaaagcgg caggcatcag tgacagaagc atccattccg
    10561 ggctgcggcc atccgatatg gccgccctgc tcaaagcgat tgagggttct ccaaaggcaa
    10621 aagcgcttaa cgatttggtt ttgcgttcca tggcgaaagc aaaatacagc ccggagtgcg
    10681 cagggcattt cggcctgtct ctaaagaatt actgccattt tacttcgccc atccggcgtt
    10741 atcccgatct tgccatacac cgtattcttg gtgactgcct gaaaaacggg aatgatgata
    10801 agctgaaaaa gcggtatggt tcgtttgttg aagaggcgtc gatccattct tccgaacggg
    10861 aagtggccgc gatgcagctg gagtgggact gcgaggcgat ttataaggcc gagtatatga
    10921 cggcccatat cggggagcag tttatcggca ccgtgacttc agttaaatcg tttggaatgt
    10981 atgtaacgct ggaaaataca gtcgaaggcc tggtgagggt ggaaaatctg ccgggcggct
    11041 ggtatgacta cgacgaagcg tccatatccc tctactgcgc acagaacggc cgccgttata
    11101 ccatcggcga cgaggtaaaa ataatcgtgg caaaggctga ggttgccacc ggacggatcg
    11161 actttgaagt tctctgaacc ggctgccgga gtttaaaaag atcatcaggc ctgtcaaaag
    11221 cgtccccgaa ttgttcgggg acgcttttga tttcggcggg aaagattcag cgaccggtaa
    11281 atagcttgtt tttacagccg ccgcttgtct cgcagtcatg ggaattcagg cagcttggct
    11341 ttgactggcc tgtacgcgat atttccactg caacattttt cccgatgacc ggacaatatc
    11401 tttcaacgac cgtcttgatt tcctgattac tcattttaac tatgatcctc cactttagtt
    11461 taaaaaaaca ataagaatat ggggctgaat cgactggtat tatttccatt gtatgcggtt
    11521 cctgccggga tatcacttta tttaatatgg gttattattt tttattataa aaccatacat
    11581 gccgaccatt tgtttattat gcgtatgaag aattcagata taccggctga tagttacata
    11641 ttttattcaa aaatggaata aatagttata tcccgtccaa aatatttcat agaatagatt
    11701 gaaaggcgga gtggttatgg aaggtgcgat agcaggtgtg atccaaatga cgctgaaccg
    11761 taaacccgaa gtgagtcctg agcagcagct gatctgtgaa ctggaagctg tcaaacagaa
    11821 tctggatacc gtatcggaaa ggtttgaata tcagagcgat cccgaccttg tggaagcgtg
    11881 tatctatgaa atgcaggcgc ttacggcaag gtaccgatac cttacaagag aagcacgccg
    11941 gctcggactg acaaagagtg cgctgctcag cctcaggcag atttaaaaca cagacattaa
    12001 aaccggaaga aatcaaaata acaggaatgt gcaaaaaaag atagggaaat gatacggtaa
    12061 ataccggcat ttccttatct tttattactt tcccggtgat tagccggact ttcggggtgc
    12121 gaaaaacatg acacaaaaaa gggggaaaac agaatgaaaa cttatgttta tattgtgatt
    12181 gcatccgtta tctgcgcatt cctgatcgcg ctgttaagga gtaaacgtct tgtcaaaggt
    12241 atagccctga gcagtctggg gggattagcc gccctgggcg cggtggattt gtcaggtatg
    12301 ctgaccggcg tcacgctcgc gttcaacctc tggacctttt tgacggcggc tgttttgggg
    12361 ctgcctggag tagtgtctat gctgcttgtc aaactgatgt ggggtatttg agtttcaggg
    12421 agcgtcaagt ctataatgcc gcgcggcaga tgattgccgg gcggtttatt tttctctgcc
    12481 gtcatctttc tgggcgatga gcagatccct gatttccgtc agcagcgcat cggtgctgct
    12541 gaggtcttct tctttcggtt cggaatcctg cttgttgtga aatttgttca caagtttgat
    12601 cattacgaag atgctcagcg aaacggtcag aaaatccagg acggcctgga gaaaaagacc
    12661 gtatttgata ccgatgggga cgcgtctgtt aaaacgggag gggatgctaa tctgaagatt
    12721 ggcaatatcg gttttgccta agaacatgga gagcaggggc gtaataatga aattgatcag
    12781 cgaggtgacg attttattga aagcgttgcc gataatgaca ccaatggcaa gctccacggc
    12841 gtttcctttg atggcaaacg ttttaaattc ggaaatgagg gatttgatcc tgttcaagta
    12901 aatcatcctt ttttgctgtt ttacggttca tagcgccctg agcggaattt tcgtgcggat
    12961 ttcttttgac gccgtaacac ctatcattat attaaaccaa aggcaaaagt gcaaatggaa
    13021 tcttcccaat aacattaaat accagattat agaaattaga gggcagtttg attgacaagt
    13081 aaggggatcg aaagtataat atagtaaata ttcatgcctt tttatacaaa gagtgtcaaa
    13141 aatagaggga aaaagaacgg ttttttctct gtcaagggag agagcgttga atgcgcataa
    13201 tttcaaggac actatcggtt ctgctgatga tgttgtggga cagcgccgcg gtcgctggat
    13261 ctgttatcat tagttttccg ttgcggtatg gttttgaccg tgtcctgccg gttaaatata
    13321 cggaatttct aaaggaatat atcatattat ccattattgt agtgttgatc tgcaatacaa
    13381 tctgcaaatg ctatatgaat gtctggcgtc atgcgggaat caatgaatat gcccggcaga
    13441 tgatttccgt tcttttgtcg gcggcgatca tggtgtttat agattttaca agacaccttg
    13501 gtctcggtat tgagctgctg attatcatcg cgatgtttga aatgctgctg atgcttggtg
    13561 tacgcgtgtc ggtccggtct tttttctttc tgcattcgcg ggcgatgtcg atgatccgga
    13621 gaaagaaaat ggagcgggtt ctggttttcg gcgcggggga agcaggcacc aatcttgttc
    13681 ataaactgga aagccacccg gaagagaatc ggctgccaat ttgttttgtt gatgacaacg
    13741 acagcaaatg gggcaagaaa atttgcggtc tgcctgttgt cggcgggaga gacaagctgg
    13801 ttaaaaccat cagggactac a
//
LOCUS       DATALY010000316         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_412106,
            whole genome shotgun sequence.
ACCESSION   DATALY010000316 DATALY010000000
VERSION     DATALY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412106"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VHR42_08605"
     CDS             <1..862
                     /locus_tag="VHR42_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3027265.1"
                     /translation="RLRQKMGMVFQSFNLFSHLMIIENIMLGPVSLLKIPRQRAYEEG
                     IRYLEMVGLGAKATAFPDELSGGQKQRAAIARTLAMKPEIVLFDEPTSALDPTMVSEV
                     LAVIRKLATSGMTMMIVTHEMKFAHDVSSRVFYMDEKGIYEDGTPAQIFDAPQKPKTR
                     AFIKKISSFTYDIKGKNFDLYELQARVEDFLTKKMFTEQRIMNVQLICEELLAGLLLQ
                     TFPTAHIALTVEYSDISSEVTLQADFDGEAYDLKTAAAGEMPLAIIQKYAKDLYSSSA
                     GGKNTVQIVL"
     gene            879..1418
                     /locus_tag="VHR42_08610"
     CDS             879..1418
                     /locus_tag="VHR42_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027266.1"
                     /translation="MSTLNMEDVFAKIESAAEKNALLPRESNILRLLGEEMLSMTLNL
                     ADNADCSFEIWNNEKRFELRLRAMAKISPESKRDFVSLSSKKENILAKGLIGKIGSAM
                     EDYLYNVGSGNYLMFCPDPLNGYAQIWTLNDYMLNTSPEEQKEDWDGLERSILMNTAD
                     DIIIGVNNGQVEMTVKKAF"
     gene            1518..1814
                     /locus_tag="VHR42_08615"
     CDS             1518..1814
                     /locus_tag="VHR42_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3027267.1"
                     /translation="MIITKTENGAALTLELEGRLDTTTSKQLEAELGTSFVGKKEVIL
                     DFAKLVYLSSAGLRVLLAAQKLMNKQGSMVIKNVNDTIMEVFEITGFADILTIK"
     gene            1888..>3132
                     /locus_tag="VHR42_08620"
     CDS             1888..>3132
                     /locus_tag="VHR42_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3027268.1"
                     /translation="MLSVFGSTIAVLFDSVIVGNFIGPEGLAALGMAKPIYYLFMMIG
                     VLINVGAATHATIYIGKGKRDKADNMLMLALVFSLGSGIVVSAFGLCFLNPVIRFLVG
                     ESALYPMVYDNGSVMLLGGTAVILMYFPFNFLRADGRPQYGVAMFVVMAALNLLLDLL
                     FVPVFHWGMKGIAAACVLSTLAADVLGLYFTFARSGNLRLKKFHTTFSEIIKILSAGS
                     AMALNNGCNVLRTLMLNKIILGAFGSLAVTVFSLTTSIGTLSLAVLSGVTQTITPLIG
                     VFFGEKDTASIRETVRTALKTGLILTGGFAILISVFCVPICALFGVREPEAVTMALPA
                     VWLYCAGLLFAMVNNIYIAHFFTIGYTLLANLLTLLRALALVVIFAAMFAGTGNLAGV
                     WASFLAADIATFGILLAAAALIR"
BASE COUNT          803 a          696 c          794 g          839 t
ORIGIN      
        1 caggctgcgg cagaaaatgg gcatggtgtt tcagtcgttt aatctgtttt ctcatctaat
       61 gatcattgaa aatattatgc tgggcccagt cagcctgtta aaaatcccac gtcagcgggc
      121 ctatgaagaa ggaataagat atctggaaat ggtcggttta ggagcaaaag caaccgcctt
      181 tcccgacgaa ctgtccggcg ggcaaaagca gcgtgcggcc attgccagaa cccttgccat
      241 gaagccggaa atcgtcttgt ttgacgagcc gacatccgcg cttgacccca cgatggtcag
      301 cgaggtcctg gcggttatcc ggaaacttgc caccagcggt atgacgatga tgatcgtcac
      361 gcatgaaatg aaatttgccc acgatgtgtc cagccgcgtg ttttatatgg acgagaaagg
      421 catttatgaa gacggcacac cagcacaaat ttttgatgct ccgcaaaaac caaaaacacg
      481 ggcatttatc aagaagattt cttcctttac ctatgacatt aaggggaaaa atttcgactt
      541 atatgagctg caggcaagag ttgaggactt tttaactaaa aagatgttta ctgaacagcg
      601 gattatgaat gtccagctga tctgtgagga gcttctggcg gggctgctgc ttcaaacctt
      661 tcccacggcg cacattgcgc ttacggtaga gtattccgat ataagcagtg aggtcacgtt
      721 gcaagcagat tttgacgggg aagcttatga ccttaaaacc gctgccgccg gcgaaatgcc
      781 gctggcaatc attcaaaagt atgccaaaga tctatactca tcctcggcgg gaggaaaaaa
      841 tacagtgcaa attgtactgt aaaaaaagga gagcagccat gagtacacta aatatggaag
      901 acgtctttgc caaaattgaa agtgccgccg aaaaaaacgc tctgcttccc agagaaagta
      961 atattttgcg ccttctgggc gaggaaatgc tgtccatgac cctgaatctg gcggacaacg
     1021 ccgattgttc ttttgaaatt tggaacaacg aaaagcgttt cgaattgcgt cttagagcca
     1081 tggcgaagat cagcccggaa agcaaacggg attttgtatc tctcagctcc aaaaaggaga
     1141 atattctggc caagggtctg atcgggaaaa taggctctgc catggaggat tatctgtata
     1201 acgtcggaag tggaaattat ctgatgtttt gtcccgatcc cctgaatgga tatgctcaga
     1261 tatggaccct aaacgattac atgctgaaca cctcaccgga agagcaaaaa gaggactggg
     1321 acgggctgga acgctctatt cttatgaata ctgccgatga cattatcatt ggtgtaaaca
     1381 acggtcaggt tgaaatgact gtgaagaaag ctttctgaac tctcagctga actgtatcta
     1441 tggtcagatt aaaattatat tattaatgcc gtagtagtag taaaataaaa atctaataaa
     1501 ttttggaggg aattattatg attattacaa aaacggaaaa cggagctgca ctgacgctgg
     1561 aactggaggg gcggctggat actacgactt caaagcagct ggaagcggaa cttggcacat
     1621 cctttgtggg gaaaaaagag gtcatactag attttgccaa actggtgtat ctttcatccg
     1681 ccgggctgcg cgttcttctc gcggcccaga agctgatgaa caaacaaggc agcatggtga
     1741 ttaaaaatgt aaatgacacc attatggaag tatttgagat taccggtttt gcggatattc
     1801 tcacaattaa ataagatgaa tgccttagca gaaaataacc gacatttttt tattcattcc
     1861 accttcaaac ggtacctgat tccgtctgtg ctgtccgttt tcggatcaac gatcgccgtg
     1921 ctgtttgaca gtgtgattgt cgggaatttt atcggtccgg aaggactggc ggcgcttggc
     1981 atggccaaac caatttacta tctgtttatg atgatcggcg tgcttatcaa tgtgggagcc
     2041 gccacccacg ccacgatcta tatcggaaaa ggcaaacgtg ataaagcaga caatatgctc
     2101 atgctggcgc tggtattctc cctcgggagc ggaattgtcg tatcggcttt cgggctgtgt
     2161 tttttgaacc cggttatccg ttttctcgtc ggggaaagcg ccctttatcc tatggtttat
     2221 gacaacggct cagtgatgct gttgggagga acagcggtca ttttaatgta ttttcccttc
     2281 aattttttgc gagctgacgg acgtccccaa tacggggtag ccatgtttgt cgttatggcg
     2341 gcgttgaatc ttctgcttga tcttctgttt gtaccggtgt tccactgggg aatgaaaggt
     2401 attgccgccg cttgcgtact cagcacgctg gcggcggatg tactgggact ttacttcacc
     2461 tttgcaagat ccggaaacct gcgtttaaag aaattccaca cgactttttc agagatcatc
     2521 aaaattctct ctgcgggaag cgctatggcg ctcaacaatg gctgcaatgt tttgcgcacg
     2581 cttatgctca acaaaatcat tcttggggct ttcggttccc tggcggtgac ggtgttttcc
     2641 ctgaccacat cgattggaac actgtcactg gcggttctgt ccggcgtgac gcagaccatt
     2701 acgccgctca tcggtgtctt cttcggagaa aaagacaccg ccagtattcg ggaaaccgtg
     2761 cgtacggccc tgaaaaccgg tttgatttta accgggggct ttgccatact gatcagcgta
     2821 ttctgcgtgc caatttgcgc tttgttcggt gtacgggaac ctgaagcagt aacgatggcc
     2881 ctgccggcgg tctggctata ctgcgctggt ctgcttttcg ccatggtcaa taacatttac
     2941 attgctcact tttttactat aggatacact ttgctggcaa atcttctgac acttttacgg
     3001 gctttagcgt tggtggtgat ttttgcggcg atgttcgccg gaactgggaa tctggcgggt
     3061 gtgtgggcct cctttttggc tgctgacata gcaacctttg gcatcctgct ggcggccgcc
     3121 gcgctgatac gc
//
LOCUS       DATALY010000317         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_243818,
            whole genome shotgun sequence.
ACCESSION   DATALY010000317 DATALY010000000
VERSION     DATALY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243818"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="zupT"
                     /locus_tag="VHR42_08625"
     CDS             <1..679
                     /gene="zupT"
                     /locus_tag="VHR42_08625"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEX3027269.1"
                     /translation="FALGFSAGVMVLVSFSELLPDSRRNLEVSFQGAAGGFITVACLL
                     AGVLAAAAIDRLVPSIEDPPSGKRHADLSGTALRRMGLVTAIAITVHNFPEGIATFMA
                     GYSNIKIGLPVALSIAMHNIPEGIAVAVPIYYGTGSRLKAFGYSAFSGLSEPLGALLA
                     YLILAPLMSGVTLGIIFGAVAGIMIYISFRELIPASERYGHTVWAFAGVLSGVLAMWC
                     ALSLFGI"
     gene            1000..3324
                     /locus_tag="VHR42_08630"
     CDS             1000..3324
                     /locus_tag="VHR42_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX3027270.1"
                     /translation="MKAVIMAGGEGTRLRPLTCDCPKPMARIVGRPILEYILDLLQQA
                     GITEAAVTLKYLPRKITGAFPNSEYHGIRLRFFEEDKPLGTAGSVRNASGFLDEDFVV
                     ISGDALCDFDLKAAFEFHREKKSAATLLLSHVADPREYGLVVTSPSGAIRGFVEKPGW
                     AQAVTDAVNTGIYIIHPDVLQLIPEGKNYDFAKDLFPLMLSRSMPVYGFEADGYWCDI
                     GDIGAYTSCQFDALEGRVVCGLPGSKKDGVVSKTQPPAGNFRLIPPVYIGEGVKIGDY
                     SIIGPNAVIDDGCTLGNSVTMKDSILLPNALVGDRSELRGALVCPGASLERGAGMFEG
                     SVAGSNSVIGRDAAVSPGVRIWPGKRVEDGARASRNLKSGVARRGIFDDEGISGEVGV
                     ELTPEFCAKIGAAAGGTFEGARVGVGDDGSNVGRSLKNAVSSGILSAGGQVCDFSSSF
                     EAMFAFAVSFCGLDMGIFTRAAGSRAVLKIVGKNGLTVGRQTERKMETAVNTGEISRC
                     APRKYGEPSNMSGIRVIYQKQLLGGAAGGLSGIDVTVHSPNRAVQKLMAETLAILGAG
                     SANSGGLRLHITAAGNDASFYDENGDYLDPGRTLALGCLIAFENGEDVALPYEAPRTV
                     DAIADRYSRKVIRYLDCPADASDHDARILAAKQPWLRDGLMGAVRILDYLKKKNSRLC
                     DAVHDIPGFAVSVKAVKIIGNPGNLLRSYAQKAEKSEGPSEGVSIRSGRGHVLLSPLK
                     QGGGLRILAEAADMEAAEELCADYEKRIRDDPRA"
     gene            3876..>4102
                     /locus_tag="VHR42_08635"
     CDS             3876..>4102
                     /locus_tag="VHR42_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027271.1"
                     /translation="MTTNSNNTNSGANNAAAGSNNANTSTGKTNNTAAKKPRNPHPAN
                     RAANQTNQTSNRANHTATPANHAATPAHHAA"
BASE COUNT          936 a          996 c         1218 g          952 t
ORIGIN      
        1 ctttgctctg gggttttcgg ccggagtgat ggttctggta tctttttctg agctgcttcc
       61 cgattcgagg agaaatctgg aggtcagctt tcagggcgct gcagggggat ttataaccgt
      121 ggcctgcctg cttgccgggg tgttggccgc cgcggccatc gaccggcttg ttccttcgat
      181 tgaagatccg ccgtcgggga agagacatgc cgacctttcc ggaaccgcgc tgcggcggat
      241 gggtcttgtg acagccatcg ccataaccgt ccataatttt ccggaaggaa tcgctacttt
      301 tatggcgggc tattcgaaca taaaaatcgg cctgccggtg gcgctgtcga ttgccatgca
      361 taatattccc gagggcatcg ccgttgccgt tccgatttat tacggaaccg ggagccggct
      421 gaaagccttc gggtattctg cgttttcagg cctttccgag ccgcttggag cgctgctcgc
      481 ctatctcatt ctcgctccgt tgatgagcgg agtgacgctt ggaattattt tcggcgccgt
      541 tgccggaatt atgatttata tctcattccg cgaactgatc cccgcgtctg aacgatacgg
      601 tcatacggtt tgggcttttg cgggcgtttt gtcgggcgtt ctcgccatgt ggtgcgcttt
      661 gtcacttttc ggtatctgaa tggttccccg cggaacagga tgaaacgtgg attatttgta
      721 aaaacagtct gcttgcgatt gacagtgtga agtatgaaaa cctttttgcg gtgtaataat
      781 aaaactgaaa caacaggacg cggagtatta tagcggtcaa acgattaaac ggcaacgatt
      841 cggctgccac aggtcacaac aaggataaaa gagggtgtgt ctgtaaaacc gaattttagg
      901 gtatggccgt ctgaccgttt gtgtggaaac cacggaaaat cgccaatagc aatttgcctg
      961 aaaagcaaat tgctataaaa ctgcaaagag gtgtttttat tgaaagccgt tatcatggcg
     1021 gggggagaag ggacaaggct tcgtcccctg acttgcgact gcccgaaacc aatggcaagg
     1081 atcgtcggcc gtccgatctt ggaatatatc cttgatttac ttcagcaggc cggtataacc
     1141 gaagcggccg tcacgcttaa atatcttccc cggaaaatta ccggggcatt tcccaactcc
     1201 gaatatcacg gaatccggct ccgctttttt gaggaagaca agcctctggg aacagccgga
     1261 agtgtccgaa acgcctctgg atttctggat gaagatttcg tggtgatcag cggcgacgcc
     1321 ctctgcgatt tcgatttaaa ggcggcattt gaattccaca gggagaaaaa gtcagcggcg
     1381 acgcttcttt tgtcccatgt ggccgacccc cgggagtatg gccttgtggt gacttcgcca
     1441 tccggcgcca ttcgcggatt tgtggagaag ccggggtggg cgcaggctgt taccgacgcg
     1501 gtcaacacgg gtatttatat catccatccg gatgttctgc agctcatccc cgaggggaaa
     1561 aactatgatt ttgccaagga cctcttcccg ctgatgcttt cccgctctat gccggtttac
     1621 ggttttgagg cggatggcta ctggtgcgat atcggcgata tcggggcgta tacctcctgc
     1681 cagtttgacg cgcttgaagg cagggttgtc tgcggcctgc cgggaagcaa aaaggacggc
     1741 gtggtcagca aaacgcagcc tcctgcggga aatttccggc tgattccccc cgtttatatc
     1801 ggcgaggggg ttaaaatcgg agactattcc atcattggcc cgaacgcagt gattgacgac
     1861 ggctgcacat tgggcaacag cgtaacgatg aaagactcaa ttctactgcc gaacgctctt
     1921 gtcggagacc gcagtgaact gcgcggagcc ctggtctgcc cgggcgcttc tctggaacgt
     1981 ggagcgggaa tgtttgaagg atcagtcgcc gggtcgaaca gtgtcatcgg cagagacgca
     2041 gccgtcagcc cgggcgtgag aatctggcct ggtaagcgcg tggaggatgg ggcgcgagca
     2101 tcgagaaacc tgaaatccgg cgtagcccgc agggggatct ttgacgacga aggcatctcc
     2161 ggggaagtcg gtgtcgagct gacaccggag ttttgcgcaa aaatcggcgc ggcggcgggc
     2221 ggaacgttcg aaggggcccg cgtcggcgtg ggagacgacg gcagcaatgt gggcaggtcg
     2281 ctgaaaaacg ccgtgtcttc cggaattctt tccgccgggg ggcaggtctg tgatttttcg
     2341 agcagctttg aagcaatgtt tgcttttgcc gtgtcctttt gcggccttga tatggggatc
     2401 tttacgagag cggccggaag ccgggcggtt ttgaaaattg tcggtaaaaa cggcctgact
     2461 gtcgggcggc agaccgaaag aaaaatggag accgcggtga acaccggtga aatcagccgg
     2521 tgcgccccga ggaaatacgg cgagccttcg aatatgtccg gcatccgggt gatttatcaa
     2581 aagcagcttc ttgggggagc ggccggaggg ctttccggta ttgatgtgac cgttcattct
     2641 cccaacaggg ctgtccagaa actcatggcc gaaacgctgg ctattctggg ggccggctcg
     2701 gcaaattccg gcggtctgcg cctgcatata acggcggccg ggaacgatgc atctttttat
     2761 gacgagaacg gggactatct tgatccgggc cggacacttg cgcttgggtg cctgattgcg
     2821 tttgaaaacg gcgaggatgt ggcgctgccg tatgaggcgc cgcgaacggt tgatgcgatc
     2881 gccgaccgtt attcaagaaa agtaattcgc tatctcgact gcccggcgga tgcttccgat
     2941 catgacgcca ggattctggc agccaaacag ccctggctga gggacgggct gatgggagct
     3001 gtgagaattc ttgattatct gaagaaaaag aattcgcgtc tgtgtgacgc ggttcacgat
     3061 attcccggtt ttgctgtttc ggtcaaagcg gttaagatta ttggcaaccc gggcaatttg
     3121 ctgcgcagtt acgcgcagaa agcggagaaa tccgaaggcc cgtcggaagg agtctctatt
     3181 cgttcgggaa ggggccatgt gcttttaagc ccgctcaaac agggcggcgg gcttcgcatt
     3241 cttgccgagg ccgcggatat ggaggcggct gaggaacttt gcgcggatta cgaaaaaaga
     3301 atccgcgacg atccacgggc ttgacaggaa tttaaagtaa agttaaaata aaaataatat
     3361 tcgtgtccgg tttttgtttt taaagaatat gcgttccgat ttttcagcac ggggaattgt
     3421 tgccgaacag tgtctttgaa tctgtccgat ggggttcaaa gacaaacagc cggcgatcaa
     3481 acggcaaact tcttttcgtg cgggaaaagg gcatttatgc gtgacgcaga aacgcggcgg
     3541 cgtttattcg tttctgtatt ctccagagtg ccagataagg aatgcggcga atgcaattga
     3601 tggaatctat taaagacccg gcttgaataa cggtctgccc gttggcattc cgtcagaacc
     3661 ggcttaatgg aaaccgactg ttttttcagg gtagaagtgt tttttttccg gtgttggatc
     3721 aaagcgcgta ccctgccctt aaagggtgaa acggattcgg tttcccgaca gcgcgcgaag
     3781 gcgcatgaat cggcatttgc cgatgccttt gagatggaat gttttttcgg acgcagccaa
     3841 agcaaaaaag gagtaaaatt gatggaggta attaagtgac aactaattcg aacaatacga
     3901 attccggcgc caataatgcg gcggcgggct ctaacaacgc aaatacaagc actgggaaaa
     3961 caaataatac tgcggcgaaa aaaccgagaa atccccaccc ggcgaaccgt gcagccaacc
     4021 agacgaacca aacgtccaac cgggcgaacc atacggccac cccggcaaac cacgcggcca
     4081 ccccggcaca ccacgcggct aa
//
LOCUS       DATALY010000318         9032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_412363,
            whole genome shotgun sequence.
ACCESSION   DATALY010000318 DATALY010000000
VERSION     DATALY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412363"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="cimA"
                     /locus_tag="VHR42_08640"
     CDS             <1..1179
                     /gene="cimA"
                     /locus_tag="VHR42_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX3027272.1"
                     /translation="DAEYFFDGFRADPGYAFAALSAAARGGADVLCLCDTNGGSYCSD
                     LGEITAKAVGAFDARVAIHCHNDMGLAVANTMAAVDRGATQVQGTFIGFGERCGNTSL
                     CTVIPNLQIKRGCDCIPEENMTMITSVSRKIAEIANVTLANNEPYVGVSAFAHKGGMH
                     IDGVRKINRSFEHISPDLVGNERRFLMSEVSGRTTVLTEVNKLDPEIHKDSEQTRRIM
                     ARIKELEHFGYQFEAAQASVELLIRKELGRYSPFFKLEHYSILGEQPVENENECSTAV
                     IKILVDGKTEITAAQGDGPVHALDSALKKALMVFYPSLSDVRLTDYKVRVMEPKYATA
                     SKVRVLIESSDGTHLWSTVGVSRDIIDASWIALVDSIDYKLTLEREKRESGRGFRRGG
                     "
     gene            1352..2611
                     /gene="leuC"
                     /locus_tag="VHR42_08645"
     CDS             1352..2611
                     /gene="leuC"
                     /locus_tag="VHR42_08645"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX3027273.1"
                     /translation="MAMTMTQKILADHAGLDNVRAGELIQARLDLVLGNDITSPVAIK
                     EFEKAGVETVFDRNKIALVMDHFTPNKDIKAAEQCKFVREFALKHQIVNFFDVGEMGV
                     EHALLPEKGLTVAGDLIIGADSHTCTYGALGAFSTGVGSTDMAGGMARGKCWFKVPSA
                     IKFVLEGKTSKWVSGKDVILHIIGMIGVDGALYKSLEFTGPGLKNLSMDDRLCMANMA
                     IEAGAKNGIFEVDEVTRQYVREHSDRECRVFKADDDAEYDKVYTIRLDQIKPTVAFPH
                     LPENTKTIDECGTVEIQQVIIGSCTNGRIEDLRSAAEILKGRKVAKGVRCIVIPATQK
                     IYLQAVEEGLVKIFVESGCAVSTPTCGPCLGGHMGILADGERAVATTNRNFVGRMGHT
                     GSEVYLASPAVAAASAIAGKITGPDNQ"
     gene            2627..3124
                     /gene="leuD"
                     /locus_tag="VHR42_08650"
     CDS             2627..3124
                     /gene="leuD"
                     /locus_tag="VHR42_08650"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3027274.1"
                     /translation="MNTTVKGTVHKYGNNVDTDVIIPARYLNTSDYKELATHCMEDID
                     QDFVTKVRAGDIMVAGRNFGCGSSREHAPIAIKESGIACVIASTFARIFYRNSINIGL
                     PIIECDEASDKIQNGDEVSIDFDTGVITNLTRSETYKGVPFPEFIQNIIDNNGLINAI
                     KNKKL"
     gene            3136..4206
                     /gene="leuB"
                     /locus_tag="VHR42_08655"
     CDS             3136..4206
                     /gene="leuB"
                     /locus_tag="VHR42_08655"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX3027275.1"
                     /translation="MEYNIALIKGDGIGPEIVDEAVKVLNRVGEKFGHAFHYTEVLMG
                     GCAIDQTGSPLPEETVEICKKSDSVLLGAVGGPKWDTLPGKLRPEKGLLGIRSALGLY
                     ANIRPALLFPELRDACPLRADISGKGFDFVVVRELTGGIYFGERGRRTGSNGLEAYDT
                     ECYSVMEVERIAGIAFDMAKKRRRKVVSIDKANVLESSRLWREVMHRIAAENPDVEYA
                     DMLVDNAAMQLVRNPGQFDVIVTSNMFGDILTDEASMITGSIGMLPSASLGGGKLGLY
                     EPIHGSAPDIAGQGKANPLATILSAAMMLRYSFGLSDEAAAIEKAVAKTLRDGWRTAD
                     ISGTNTVPVNTGKMGELVVSNL"
     gene            4685..5725
                     /locus_tag="VHR42_08660"
     CDS             4685..5725
                     /locus_tag="VHR42_08660"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX3027276.1"
                     /translation="MKQVIIPIDECSEGDVLAADVLNNSGVKLVTSQTVLNIFIINKL
                     ADFGVDRISVFNPPLITKTREYSYKKFIGNYKESVLLVKDMIHELSAGKRIDYNNIVS
                     VSDIIYSHVEENANIINCLKQMRGSDEYTYSHSVNTSFYSMLISKWLGFSEDDIMKAL
                     QAGFLHDIGKSKIPLEILNKTGPLTGKEFSIIKKHPIYGYYILHENKHIDFDIKRAVL
                     LHHERENRSGYPFRLSTDSIGSFARIVSVADVYDAITSDRVYKKKSTPFKAFEMFLTE
                     GVRQFDSTVINAFISNLSTYLIGSEVLLSTGETGKIAYIPPTNVTAPIIQVGDNYVEL
                     SNGGEITIENML"
     gene            complement(5786..6901)
                     /locus_tag="VHR42_08665"
     CDS             complement(5786..6901)
                     /locus_tag="VHR42_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HEX3027277.1"
                     /translation="MSKKISVLVFAAIILLIPLASVLSPYRTISEDENRALTSFPVFN
                     FDEFTSKRFMHNFDSFVSDHIVLRDGWTTVKAEATTLLGKRDNKGVYLGDNCLIENLS
                     SPKESLWRPNLEAINTFAARTRNPAFLLLAPTAGEIERDKLPPYAQTYNQASFIRSVG
                     RQLKGVSLIDTVKNLSAHKSEYIYYRTDHHWTSLGAYYAYSAAGKLLGYDPLPKSGFR
                     IERATTAFNGTLYSKSGYRNITPDAIDFYTPKSGPVLKQLNIGLGSKPKQYTSIYFRN
                     FLKVKDKYSVFFDGNQPLEDIRTNSVGKRLLVIKDSYAHSLVPFLMNHYSRITMVDTR
                     YLLQPLDQTVNLRNYDQILFVYNVDTFATDQTIPNIN"
     gene            complement(6932..8338)
                     /locus_tag="VHR42_08670"
     CDS             complement(6932..8338)
                     /locus_tag="VHR42_08670"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002137128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX3027278.1"
                     /translation="MVFSSLTFLYVFMPIVMSVYYIAPKRLRNLVLLIEGMVFYAWGE
                     PVYIIVVMASTIMDYIYGRLMDRHKENRRMMKFLIVISVVSNLGILAVFKYSSFLIGN
                     LNYFFHLSIPDPHIPLPVGVSFHTFQSMSYTIDMFFGNIAVQRNLVNFCTYVTLFPQM
                     VAGPIVRYSEVEHEIDKRVINLDTLDQGVKLFIRGLAKKVLLANNIGAAWSAVQSQQA
                     SSLTLVQSWFGILCFTFQIYFDFSGYSDMAIGLGKMLGFSFPRNFNYPYLSKSITEFW
                     RRWHITLGMWFRNYVYIPLGGNRCSRLKQIRNLLIVWFLTGLWHGASWNFVIWGVYFG
                     VIIILEKFVLHSLLEKIPTPLRIVGTFCLIVCGWVFFEFESLPRAISFFGVMFGVHGA
                     GVINSATIYQLYNNALIFIICAVFSTDLPAKGYRYLKQRAEKTVGWLTPVAHAAVMLL
                     STAFLVDASYNPFLYFRF"
     gene            complement(8371..8850)
                     /locus_tag="VHR42_08675"
     CDS             complement(8371..8850)
                     /locus_tag="VHR42_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HEX3027279.1"
                     /translation="MVQFRRAAAILCAAILLLTFGCSSDPTHGKKPKPSEIVSKIQKD
                     IKFPEMLSVKQERLSRYYSVSSGLLDDFSVSICSSAASSSEVAVFKGKTTADAVKIKD
                     AVNKRLDQKKGVFENYGEPQEYSNLKNCVVDTKGPYVFFAVCGDSAKARSDFNSFFN"
BASE COUNT         2404 a         2052 c         2413 g         2163 t
ORIGIN      
        1 gacgccgagt atttctttga cggcttcagg gccgacccgg gttacgcgtt tgcggccctc
       61 agcgcggccg cgagaggggg cgccgacgtt ctctgccttt gtgacaccaa cggcggcagc
      121 tattgctccg atctggggga gatcactgcc aaagcggtcg gggcgtttga cgccagagtg
      181 gcaattcact gccataacga catgggtctc gccgttgcca acacgatggc ggccgtcgac
      241 cggggcgcga cacaggttca gggtacgttt atcgggtttg gggaacgctg cggaaacacc
      301 agtctctgca ctgttattcc gaatcttcag attaaaaggg gatgcgactg cattccggaa
      361 gaaaatatga cgatgatcac ttccgtatcg aggaagattg ccgaaattgc caatgtcaca
      421 ctggcgaaca acgagcctta cgttggcgtc agcgccttcg cacacaaagg cgggatgcac
      481 atcgacggcg tgcgcaagat caaccgttcc ttcgagcata tttcaccgga tcttgtgggc
      541 aatgaaagac ggttcctgat gtccgaagtc agcggccgca cgacggtcct gacggaagtc
      601 aataaactcg atcccgaaat ccacaaggat tctgaacaga ccaggcgaat catggcgcgg
      661 attaaggaac tggagcattt tggctatcaa tttgaggccg cgcaggcgag cgtcgaactt
      721 ctgatccgga aagagctggg ccgatacagc ccgtttttca aactggagca ttacagtatc
      781 ctcggcgagc agccggtgga aaatgaaaac gaatgctcaa ccgccgtgat caaaattctg
      841 gttgacggaa agactgaaat tactgccgcc cagggtgacg gcccggttca cgcccttgac
      901 agcgcgctca aaaaggccct gatggtattt tacccgagcc tgtcggatgt gaggctgacc
      961 gattataagg tcagggtcat ggaaccgaaa tacgcaaccg cttccaaagt gcgtgtgctg
     1021 atcgaatcgt ccgacggaac tcatttatgg tcaacggtgg gcgtatctcg cgatattatc
     1081 gacgcgtcat ggattgcgct ggtggattca attgattata aactcaccct cgaaagagaa
     1141 aaaagagaaa gcgggcgcgg tttccgccgg ggcggatgat tgtcaatcac ggaccgggcg
     1201 tgtataatag aagtgaaaaa aacgctgttt taccgcgtta acgcaaccaa acggctgtta
     1261 gaataaaaca tgtgaagcaa atgcccccaa attccgccgc aggggggtgt tgatgcaacc
     1321 gcagcggcag cggaaggacg gagtatcgac tatggcaatg acgatgactc aaaaaatact
     1381 ggccgaccac gcgggactgg acaatgtccg cgccggggag ctgatacagg cacggctgga
     1441 tcttgtgctt ggcaacgata tcacctcgcc ggtggcaatt aaggaatttg aaaaggcggg
     1501 tgtggagacg gtctttgacc gcaataagat cgcccttgta atggatcatt ttacgcccaa
     1561 taaagacatc aaagcggccg aacagtgtaa atttgtccgt gaatttgcgc tcaaacatca
     1621 gatcgtcaac ttttttgacg tgggcgaaat gggtgttgag catgctcttc tccctgaaaa
     1681 aggtcttact gtggccgggg atctcatcat cggagccgat tcccatacct gcacctatgg
     1741 cgcgctgggc gctttttcaa ccggcgtggg cagcacggat atggccggag gaatggccag
     1801 agggaagtgc tggttcaagg tgccgtcggc aatcaaattt gttctggagg gaaaaacatc
     1861 caaatgggtg tcgggcaagg acgttatcct gcatatcatc ggtatgatcg gggtggacgg
     1921 cgcgctctac aaatccctcg aatttaccgg cccgggcctt aaaaaccttt cgatggacga
     1981 ccggctctgc atggccaata tggcgattga agcgggagcc aagaacggta tttttgaagt
     2041 cgatgaggtt acccggcaat atgtgcgcga gcattccgac cgggaatgca gggttttcaa
     2101 agccgatgac gacgcggagt atgataaggt ttacacgatc cgtcttgatc agatcaaacc
     2161 gaccgtggcg ttcccgcatc ttcccgaaaa tacgaaaacc atcgacgagt gcggtacggt
     2221 ggaaatccag caggtgatca tcggttcctg caccaacgga agaattgaag atctaaggtc
     2281 ggcggctgaa attctcaaag gccgcaaagt ggcgaagggt gtccgctgca tcgtcatccc
     2341 cgcgacgcag aagatttatt tgcaggctgt tgaggaaggg ctcgtaaaga tttttgtcga
     2401 gtcgggctgc gccgtttcca ctcctacctg cgggccgtgt ctgggcggtc acatgggtat
     2461 tctggccgac ggcgaaaggg ctgtcgcgac caccaaccgc aattttgtcg gtagaatggg
     2521 gcatacgggc tctgaagtat acctggcaag ccctgcggtt gccgccgcgt ccgccatcgc
     2581 cggaaaaatc accggccctg acaatcaata aaaggagggt tatggaatga atacgactgt
     2641 aaaaggaacc gttcacaagt acgggaataa cgtcgacacc gacgtgatca tccctgcaag
     2701 atatctcaat acatcggatt acaaggaact ggctacccac tgcatggagg atattgatca
     2761 ggatttcgtg acaaaggtca gagcgggcga cattatggtg gccggcagaa acttcggctg
     2821 cggttcttcc cgggagcatg ctcccattgc aatcaaggag tccggcattg cctgcgtaat
     2881 tgcctcgacg ttcgcgcgga ttttctaccg caattccatc aacattggtc tgccgatcat
     2941 cgaatgcgac gaggcttctg ataagataca aaacggggac gaggtatcca ttgattttga
     3001 caccggcgtc attaccaatc tcacaagatc ggagacctac aagggtgttc cgttcccgga
     3061 atttattcag aacattatcg acaacaatgg cttgattaac gccataaaaa acaaaaagct
     3121 ataggaggcc ttggcatgga gtacaatatt gccttgatca aaggggacgg aatcggcccg
     3181 gaaatcgtgg acgaggctgt gaaagtgctc aacagagtcg gtgaaaaatt cggtcacgct
     3241 ttccactaca cagaggtgct gatgggcggc tgcgcgattg atcaaacagg atcgccgctt
     3301 cccgaagaaa cagttgaaat ctgcaaaaag agcgacagcg ttctgctcgg cgcggtcggc
     3361 gggccgaagt gggatacgct tcccggcaaa ctgcgccctg agaagggact gcttggaatc
     3421 cgctccgccc ttggccttta cgccaacata cggccggccc tgctgttccc cgaacttaga
     3481 gacgcctgcc cgctgcgcgc cgatatttcc ggcaagggct ttgactttgt cgtggtccgc
     3541 gagcttacag gcggaattta tttcggtgag cgcggcagaa gaacggggag caacggtctc
     3601 gaagcctatg acacggaatg ttacagcgtg atggaggtcg aaaggatcgc cggaatcgcg
     3661 tttgatatgg caaagaaacg cagaaggaaa gtcgtaagca tcgacaaggc gaatgttctg
     3721 gaaagctcgc ggttatggcg tgaagtgatg catcgtatcg cggccgaaaa tccggacgta
     3781 gaatatgccg atatgcttgt cgacaacgcg gccatgcagc tggtcagaaa tccgggccag
     3841 tttgatgtga ttgttaccag caatatgttt ggcgacattc ttacggatga ggcaagtatg
     3901 attaccggct cgatcggcat gcttccctcc gcctcgctcg gtggcggaaa gctcggttta
     3961 tacgagccga tacacggatc ggcccctgat attgcgggcc agggcaaggc aaatccgctt
     4021 gcaacgatcc tttccgccgc catgatgctc cggtattcct tcgggctttc cgatgaagcc
     4081 gcagccattg aaaaagcggt ggctaaaact ctgcgtgacg gatggcgcac ggcggatatt
     4141 tccggtacga ataccgttcc cgtgaatacg ggaaaaatgg gagaactcgt cgtgtcgaac
     4201 ttgtagccat ctatccccag cggaattctg acgaatcttt ttcattcgca aagggcttcc
     4261 cgccatgtgt tcggcgcgga agcccttttt gctacaatcg ggaagcggat aaaaattttg
     4321 agattcggac ggaatgatcc ttctatttga tttaaaaaag catctggata aaaatcggag
     4381 ggtgtcttac aggctgttta ttgattttgg ctatattaga ttaaattagc acagcaaaat
     4441 ttaaagtttc ggttatttaa ttttaaatta cttttaaatt tcgacataat tgtatataat
     4501 atactataat cagaaaaaaa tatatcgaga tatatcgaga atgttgatca taatcttgtt
     4561 tatctttgga attgtttatg ataagataag aaatgacgaa ataaatgttt tttggaagtg
     4621 aaaagtgcaa caagtagaaa cgattctgcc aaatatgcat aattgtttca ggagagtgat
     4681 ttttttgaag caggttatca tcccaatcga cgaatgcagt gaaggcgatg tccttgctgc
     4741 cgacgtcctc aacaacagcg gtgtaaagct ggttaccagt caaacggtat taaatatatt
     4801 tatcatcaat aaacttgccg actttggtgt agacagaatc agcgtcttta atccgccgct
     4861 tattaccaag acccgtgaat attcctataa aaaatttatc gggaattata aagaatctgt
     4921 tcttcttgtg aaagatatga ttcatgagct ttcagcaggc aaaagaatcg actataataa
     4981 tatcgtgagt gtttccgaca tcatttacag ccatgtggaa gaaaatgcga atatcatcaa
     5041 ttgcctcaag cagatgcggg gcagcgacga atacacctat tcccatagtg tcaacacgtc
     5101 tttttattcc atgctgatca gcaaatggct gggcttttca gaagatgata tcatgaaggc
     5161 gctccaggcg gggtttcttc acgacattgg aaaatccaaa attcctcttg aaattcttaa
     5221 caagaccggc ccgctcaccg gcaaggaatt cagcattatc aaaaagcatc ctatttacgg
     5281 atactatatc ctgcacgaaa ataaacatat tgattttgat attaaaagag ctgttctcct
     5341 tcatcacgag agggagaacc gttcaggcta tccttttcgt ctgtccacag acagtattgg
     5401 gtcttttgcc cgaatcgttt cggtggccga tgtttatgac gcaatcactt ccgatcgggt
     5461 ttataaaaag aagtcgaccc ctttcaaggc gtttgaaatg tttctgaccg agggtgttag
     5521 acaattcgac agcacggtca ttaatgcttt tatcagcaat ttgtcgactt atctgatcgg
     5581 atctgaggtc ttgctgagta ccggagagac gggaaagatt gcctatattc ctcctacaaa
     5641 tgtaactgcc ccgattatac aggtcggcga taactatgtc gaattatcga acggcggaga
     5701 aatcaccatt gaaaatatgc tctaaggatg ccggtttcgt tcagaatgag cgggcctcag
     5761 ctgtgatgct gcggctcgct cttttttaat tgatattcgg tattgtctga tcggttgcaa
     5821 aggtatcgac gttatagaca aataagattt gatcgtaatt ccggaggttt accgtctggt
     5881 cgagcggctg gaggagatag cgggtatcca ccatcgtaat gcggctgtag tggttcatca
     5941 ggaagggaac cagagaatgt gcgtaggaat ccttgattac cagaagacgt ttgcccacgg
     6001 aatttgtccg gatgtcttca agcggctgat tgccgtcaaa gaagacggaa tatttgtctt
     6061 tgactttcag aaaattccga aaatagatac tggtatattg tttgggcttt gagccaagcc
     6121 cgatgttgag ttgtttcaaa accggcccgc ttttcggagt gtaaaaatcg atcgcatctg
     6181 gggtaatgtt tcggtagccg cttttggaat agagggttcc gttaaaggct gttgtggcgc
     6241 gctcaatccg gaaaccgctt ttagggaggg gatcatatcc aagcagtttt ccggccgccg
     6301 aataagcgta atacgcgcca agcgaagtcc agtgatggtc ggtccgataa tagatatatt
     6361 cacttttatg cgcactcaga ttctttaccg tgtcaatgag ggagacccct ttcagttgac
     6421 ggccgacgct gcggataaag cttgcctggt tataggtctg agcgtagggg gggagtttat
     6481 cccgctctat ttcgccggcc gtgggggcaa gcagcaggaa ggctggattg cgcgttctgg
     6541 cggcaaaggt attgatcgct tccagattgg gccgccagag tgattctttc ggactggaaa
     6601 gattctcaat cagacagttg tcaccgagat aaacgccttt gttgtcccgt ttgccgagaa
     6661 gcgtggtggc ttcggctttg acagtggtcc agccgtcccg aagaacaata tggtcggaca
     6721 caaagctgtc gaaattgtgc atgaatcttt ttgatgtaaa ttcgtcgaaa ttaaagacgg
     6781 gaaacgaggt aagcgcccgg ttttcatcct ctgaaattgt tcggtaaggg gagagaacgg
     6841 aagcaagggg aatcaggaga atgattgccg caaacaccag caccgatatt ttttttgaca
     6901 tgcagttcac cttttcacaa gtttcagacg gtcaaaaccg gaaataaagg aaggggttat
     6961 agcttgcatc cacaaggaac gcggtgctca gaagcatcac cgcggcatgt gcaaccggag
     7021 taagccaccc gaccgttttt tcggcccttt gtttcagata acggtagcct ttggcgggaa
     7081 gatcggtgga aaagaccgcg cagatgataa agatcaaagc attgttataa agctgataaa
     7141 tggtggcgct gttgattacg ccagcgccat gtaccccgaa catgacgccg aaaaatgaaa
     7201 tggcccgggg cagactttca aattcgaaaa agacccagcc gcatacaatc agacagaaag
     7261 taccgacgat tcgaagcgga gtcgggattt tttccagcag ggaatgcaga acaaacttct
     7321 cgagaataat gatgactccg aaataaacgc cccagatgac aaagttccag ctggcgccgt
     7381 gccaaaggcc ggtgagaaac cagacgatca gcagattgcg gatttgtttg agccggctgc
     7441 atcggttgcc gccgagcgga atatagacgt agttgcggaa ccacatgccc agcgtaatat
     7501 gccagcgtct ccagaattcc gtaatgcttt tggagagata agggtaattg aaattccggg
     7561 ggaaactgaa gccgagcatt ttgccaaggc caatcgccat gtcggaatat ccggagaagt
     7621 caaaatagat ctggaaggta aagcagagaa taccgaacca ggactggaca agcgtgagcg
     7681 aagaggcctg ctgggattga acggcgctcc acgccgcgcc gatattgttg gccagaagaa
     7741 cttttttggc aaggccgcgg atgaagagtt tgacgccctg gtcaagtgtg tcaagattga
     7801 taacgcgttt gtcaatttca tgctcgacct cgctatagcg tacgatcggt ccggcaacca
     7861 tctgtggaaa cagcgtcaca taagtgcaga aattaaccaa attgcgctga accgcgatat
     7921 tgccgaaaaa catgtcaatg gtgtaggaca tgctttgaaa agtgtgaaat gagacgccga
     7981 ccggcagcgg aatgtggggg tccggaatac tgagatgaaa aaagtaattg aggttgccga
     8041 tcagaaaaga gctgtattta aagacggcca gaattccaag attggagacg accgatatca
     8101 cgatcagaaa cttcatcatc cgccggtttt ctttgtgtct gtccatcagg cgtccgtaaa
     8161 tatagtccat aatggtggag gccattacga cgatgatata aaccggttcg ccccaggcgt
     8221 aaaaaaccat gccttcgatc agaagcacaa gatttctcag acgcttgggc gcaatatagt
     8281 aaaccgacat gacaatgggc ataaagacat aaagaaaggt tagacttgaa aacaccatgg
     8341 gtggaaaccc ctattcctgt tttttcggta tcagttaaaa aagctgttaa aatcgcttcg
     8401 cgctttggcg ctgtcgccgc agactgcgaa aaacacatag gggcctttgg tatccaccac
     8461 gcagtttttc agattgctgt attcctgcgg ttctccgtaa ttttcaaaaa cacctttttt
     8521 ctgatcaagg cgcttattga ccgcatcttt gattttaacc gcgtccgcgg tggtttttcc
     8581 tttaaaaaca gcgacttcgg aagaagacgc agccgaagaa cagatcgaaa cggagaagtc
     8641 gtctaataaa ccggaagaca ccgaataata gcgtgaaagg cgttcctgtt tgacggaaag
     8701 catttcggga aatttgatgt ctttttgaat tttcgagaca atctccgatg gctttggttt
     8761 tttgccatgg gtggggtcgg acgagcagcc gaaggtcaga agaagaatgg ctgcgcagag
     8821 gattgctgcg gcgcggcgga attgtaccat tttaatcagc tccaaagtat tttattgatt
     8881 cgcaatcagg taattaaacc atattctata tccggcaatg cgcagatgca tgccgtcgtc
     8941 gcttaagttt ggcggaagct ttccatccgg gcctttcagc gccgacgcga cgttgacgta
     9001 ttttacgccc agattagcgc acatcgtttt ga
//
LOCUS       DATALY010000319         6680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_328927,
            whole genome shotgun sequence.
ACCESSION   DATALY010000319 DATALY010000000
VERSION     DATALY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328927"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..518
                     /locus_tag="VHR42_08680"
     CDS             69..518
                     /locus_tag="VHR42_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX3027280.1"
                     /translation="MKRLPIQANDRPGRNVLLQKGVKSTKQRLAVIESLSGSESPLSA
                     QEIFIRIRTGFQHLSLSTVYRILEVLKNAGIVEKTGLMEGGGALYELNSEVHKHNLIC
                     VKCHKIIPIEGCPLGIYESNLEQRTGYKISGHKLEVYGICPKCRSGK"
     gene            681..1253
                     /locus_tag="VHR42_08685"
     CDS             681..1253
                     /locus_tag="VHR42_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX3027281.1"
                     /translation="MRRKSGLFYVMITGVALVVILLIWGIASYNSLVGLSANIESSQG
                     DIQTMLQRRSDLIPNLVSTVKGYASHETAAIKAVTDARAKLAGAKGNTDQAAASDQLT
                     SALSRLLVVVENYPNLKADANFRQLSDELAGTENRIAYTRTTYNDKVKSYNAKIRSFP
                     TNIIAGMFGFNKADYFTAAKGAETVPKVNF"
     gene            1256..2065
                     /locus_tag="VHR42_08690"
     CDS             1256..2065
                     /locus_tag="VHR42_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX3027282.1"
                     /translation="MKKLFTAVLLTAILSVALATPALAVSYPSPTSQFFVNDFVGVMN
                     AQDKQDAYEIGATLQQKTGAQVVIVTIGSLQGMDDHEYALNLANRWGIGQKGKDNGVL
                     LLLSKGDRKSRIEVGKGLEGVLNDAKTGRIQDDYILPEYKKNNYSGGLLAGYKVIVTQ
                     VYKEYGVTPPAGLKDYQLSDSDNGKGEDTVSVVFMVAILVIVILSRLGRRRGPRGPLG
                     PGGGFFGGFFGGPFIGGGGNSGGFGGFGGGSGGGFSGGGGGFGGGGSGRSF"
     gene            2131..2325
                     /locus_tag="VHR42_08695"
     CDS             2131..2325
                     /locus_tag="VHR42_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX3027283.1"
                     /translation="MKRLFKSTKSKMICGVCGGIAEYFNIDPSLIRIAWVIFGFMGGA
                     GILAYFVCAIILPNDVDLGQ"
     gene            2395..3171
                     /locus_tag="VHR42_08700"
     CDS             2395..3171
                     /locus_tag="VHR42_08700"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX3027284.1"
                     /translation="MVLTIDIGNTNIVLGGWNQDKLEFVSRLETNRFKTEDGYAIELK
                     SIFELYQIERSGVEGSIISSVVPQISGAVAHSVERLTGKKPLVVGPGIKTGLNIRVDD
                     PAHLGSDLVVDAVAALAKYPKPIIIVDMGTATKISAIDTSGSFMGCAIIPGIRLGLDA
                     LAERTAQLPHIELKTPEEMIGKNSSDSMQSGAVYGTASMIDGMVERFEQRLNQKASVV
                     ATGGFAGDIIPHCRRKITLCPHLLMEGLLMIYRKNVAAKI"
     gene            3424..6030
                     /locus_tag="VHR42_08705"
     CDS             3424..6030
                     /locus_tag="VHR42_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX3027285.1"
                     /translation="MNNAYEKMDQAAAAAELSTDLQGGLTAGQVSERLEKFGNNELHG
                     KKAKSPVVRFLSQFKDPMIYILFAATVISLFLKEFTDSFIIIVVVLVNGVIGYIQESK
                     AEKAIEALKKLSNPKALVRRGGAIQEIEADGLVPGDLVVLEAGRFVPADLRLVESSNL
                     KIEESALTGESVPVDKDSEFIAQSEVPLGDRINMAYMSTNVTYGRGSGIVTATGMETE
                     IGKIASILDKTEETMTPLQKRLADLGTVLGIVAVVLCALLFLLAVIQKRNIVEMLLTA
                     ISLAVAAIPEGLPAVVTIVLAIGVQRMVKSRSIIRKLPAVETLGSVNIVCSDKTGTLT
                     QNRMTVVECFYDNRIYKPEEMNADSASLFFDGFTLCNDASIENGEKIGDPTETALLDM
                     SALQGITRPSLEDAHPRIDEIPFDSNRKMMTTLHRYGEKKISFTKGALDSILRHTKYI
                     WRDGRAVEAIPEEVAKINQAASRMASKALRVLAVSFCESDRAVEGELIFLGLVGMIDP
                     PRPEAKQAVGVLKEAGVTTVMITGDHKETAYAIAHDLKIAEGKNQVISGDELDRLTQE
                     QLNDQVKELRVFARVSPENKVMIVNAFKNGGNIVSMTGDGVNDAPSLKSADIGVAMGI
                     TGTDVAKGAADMILTDDNFATIEKAIEEGRNIYNNIRKATLYLLASNFGEIITMFVAI
                     LIGLPSPLSPIHILWVNLITDSLPGLALGVDPGNPNIMKLKPRDPKESLFARGGVFTM
                     ILYGTVIGAITLLGFHLGYQEGGLREGRTFALTILAVSQLFHAIGMRDVDKSIFKMNH
                     LNNRFLIVSVLLGLMLQVAIAQLPYINSVFGTVALGFHQWAITILLALVPLAVHEIVL
                     LVKKATGKQIAA"
     gene            6459..>6680
                     /locus_tag="VHR42_08710"
     CDS             6459..>6680
                     /locus_tag="VHR42_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027286.1"
                     /translation="MKTKNDNPVSPRLPKIWEDSGRAEPENSGEISRARITDRSFRGL
                     TAARLVVEGSAFENIVFQDCKLPALDVQDA"
BASE COUNT         1715 a         1531 c         1854 g         1580 t
ORIGIN      
        1 attgtcggaa tattgtaatt cgggggggtc tgtgataaaa tgaagctaat cttgtattgg
       61 aaacggggat gaaaaggttg ccgattcaag ccaatgacag gccgggcaga aatgtcctgc
      121 tgcaaaaagg ggtcaaaagc accaaacaaa ggctggccgt catcgagtcg cttagcggca
      181 gtgaatctcc actgtccgcc caggaaattt tcatccgtat tcggaccggt tttcaacatt
      241 taagcttgtc gacggtttat cgcattcttg aagttctgaa aaatgccgga atcgtggaaa
      301 aaaccggcct gatggagggc ggaggggctc tttacgagct caattccgag gttcataaac
      361 ataatttaat ttgtgttaaa tgccataaaa tcattccgat tgaaggatgt cccctcggta
      421 tttatgaaag caatctcgaa cagcggacgg ggtataaaat ttcgggtcat aaacttgaag
      481 tttacggtat atgcccgaaa tgccgcagcg gtaaataatc ctgctatccg caatttggaa
      541 gagattttat catgtttatc gggatatctt tcataaaatg aaaaagaatg atataatgaa
      601 gcaaccctga aataaggcat aattcgctca taggcgacat gccgacagaa aacaacgcgc
      661 tttgcgcaaa cggaggatat atgagaagaa aatcgggact gttctatgtg atgattaccg
      721 gcgtggctct ggtcgtaatt ctgctgattt gggggattgc ttcctataac agcctggtgg
      781 gcctttcagc aaatatcgag agcagtcagg gcgatatcca gaccatgctt cagcggagaa
      841 gcgacctgat accgaatctc gtttctacgg tcaaaggtta tgcgtcgcat gaaacggccg
      901 ccatcaaagc ggtaacggac gcgagagcga aactcgcagg cgccaagggc aatacggatc
      961 aggccgcggc cagcgatcag ctgacaagcg cgctcagccg gctgcttgtg gtggtggaga
     1021 attaccccaa cctgaaggcc gatgcaaatt tccgccagct ctccgatgag cttgcgggaa
     1081 ccgagaacag gattgcttac acgcgtacca cttataacga taaggtcaaa agctataatg
     1141 caaaaatcag gagttttccg acgaatatca ttgcggggat gttcggtttc aataaggccg
     1201 attactttac ggctgcaaag ggcgcggaaa ctgtcccgaa ggtgaatttt taattatgaa
     1261 gaaacttttc acagccgttc tgctgacggc gattctttcc gtggcgctgg cgacgccggc
     1321 tctggcagta agctatccgt ccccgacatc acaatttttt gtaaacgact ttgtgggcgt
     1381 tatgaacgcg caggataaac aggatgcata tgaaatcggc gcgacgctcc agcagaagac
     1441 cggtgctcag gtcgtaattg tgacgatcgg cagtctgcag ggaatggatg atcatgaata
     1501 cgcgctgaat ctggccaacc gctgggggat cggccaaaag ggcaaagaca atggagtcct
     1561 gctgctttta tccaaaggtg atcgaaaaag cagaattgag gtcggtaaag gtctggaagg
     1621 tgtgctcaac gatgccaaaa ccggcagaat tcaggatgat tatatcctgc ccgaatacaa
     1681 aaaaaacaat tacagcggcg gccttttggc cggttataaa gtgattgtga cccaggttta
     1741 taaagagtat ggcgtaacgc ctccggccgg tttaaaggat taccagctca gtgacagtga
     1801 taacggcaaa ggggaagaca ccgtaagcgt tgtctttatg gttgctattc tggtaatcgt
     1861 gattctcagc cggttgggaa gacgccgtgg tcccagaggg ccgctcggcc ctgggggagg
     1921 attcttcggc ggtttcttcg ggggtccgtt tatcggcggc ggaggaaatt ccggtggatt
     1981 cggaggcttt ggcggcggct ccggcggagg attttcaggc ggaggcggcg gtttcggcgg
     2041 cggaggaagc ggcaggagtt tttaacggat ttattacgat cttgtatttt actcatagaa
     2101 tagaaagaaa gtaccaaagg gggtgtaagt atgaaacggc tttttaagtc aaccaaaagc
     2161 aaaatgatct gcggtgtatg cggcgggatt gccgagtatt ttaatattga cccatccttg
     2221 atccgaatcg cctgggtgat ttttggattt atgggcggcg cgggaattct ggcttatttt
     2281 gtgtgcgcaa tcatcctgcc caatgatgtt gatctgggcc agtagagtag aattcggctt
     2341 caataaatgt gcgctgcatc cggcttggga gcggcagcat gggaggaatg taaaatggtt
     2401 ctgaccatcg atattggaaa taccaacatc gttttgggtg gatggaatca ggataaactg
     2461 gagttcgttt cgcgtcttga aacaaaccgg tttaaaaccg aggatggcta cgccatcgag
     2521 ctgaaaagta tctttgagct ttatcaaatc gagcgttccg gcgtggaggg ttcgatcata
     2581 tcctctgtgg tccctcaaat atcgggagcg gttgcccatt cggtggaaag actgacgggc
     2641 aaaaaacctc tggttgtggg gcccggcata aagaccggcc taaatatcag ggtcgatgac
     2701 ccggcccatc tgggcagcga tcttgttgtt gatgcggttg ccgcgctggc gaaatatccc
     2761 aaacccatta ttatagtaga tatgggaacg gcgactaaaa tcagtgcgat tgacacgtcc
     2821 ggttccttta tgggatgcgc gattattccg ggaatccggc tggggcttga cgccctcgcg
     2881 gaaaggacgg cgcagcttcc ccatatcgaa ttgaaaacgc cggaggaaat gatcggcaag
     2941 aattcatctg acagcatgca gtccggagcg gtctacggga ctgcttcgat gattgacggc
     3001 atggtggagc ggtttgagca gcggctgaat caaaaggctt cggtggttgc gaccggaggg
     3061 tttgccggtg atattatacc gcattgtcgc aggaagatta ctttatgccc ccatctgctg
     3121 atggaagggc tgctgatgat ttaccgaaaa aatgtcgcgg caaaaatttg aatatccgct
     3181 taagacggac cgcacaaggt taagagggag tagcaattaa ttgcaaaatc aacatcacag
     3241 gccacccggc cctggttttg cagcctttac tcaaaaggta gtcaagactc ttaacatatt
     3301 tcttttcaat aagggatatg ttaagagtta ttttttgatt ttttcacttt ttttaagaat
     3361 aataatacca gatggtttga gatattatga agtgaaaagc cctacatata aggagtggat
     3421 gaaatgaaca atgcgtatga aaagatggat caagccgcgg ccgccgcaga gctttcgacc
     3481 gatctccaag gcggtttgac tgccggacag gtgtcggagc ggctggagaa gttcggaaac
     3541 aatgagcttc acggaaaaaa ggcgaaaagc cccgttgtcc gttttctgtc ccagtttaag
     3601 gacccgatga tctatatcct gttcgcagca acggtgattt ccctcttcct caaagagttt
     3661 accgacagtt ttattattat cgtcgtcgta ctggtcaacg gcgtgatcgg ctacattcag
     3721 gaatccaagg cggagaaagc catcgaggcg ctgaaaaagc tctccaaccc gaaggcgctg
     3781 gtcaggcgcg gcggcgcgat tcaggagatt gaagccgacg ggctggttcc cggcgatctg
     3841 gtcgtgctgg aggcaggaag gtttgtcccc gccgatctgc gcctggtgga atcctccaac
     3901 ctcaaaattg aagaatccgc attgacgggt gaatctgtgc cggtcgataa agacagtgaa
     3961 ttcatcgcgc aaagtgaagt cccgctgggt gatcggatta atatggcgta catgtcaacc
     4021 aatgtcacct acggcagagg ctcgggaatc gtgacggcca ccggaatgga aaccgaaatc
     4081 ggaaaaattg cgtcgattct cgacaagacg gaggagacca tgacgccgct ccaaaaacgg
     4141 cttgccgatc tcgggacagt acttggaatc gtagcggttg tcctgtgcgc gctgctcttc
     4201 ctgctcgcgg tcattcaaaa aaggaatatc gtcgagatgc tgctgaccgc catttccctt
     4261 gccgtagcgg cgatcccgga aggattgccg gccgtggtaa cgatcgttct tgcgatcgga
     4321 gtgcagcgga tggtaaaatc gcgttccatt atccgcaaac tgcctgcggt ggaaacgctt
     4381 ggctcggtca acatcgtctg ttcggataaa accggaacgc tgacccaaaa ccgcatgacc
     4441 gtggtggagt gtttttacga taacagaatc tataagccgg aagagatgaa tgcggattct
     4501 gcgtctctgt tttttgacgg ctttacactc tgcaatgacg cctcaattga aaacggcgaa
     4561 aaaatcggcg atccgaccga aaccgcgctt cttgatatga gcgcgcttca gggtatcacg
     4621 aggccgtcac ttgaggatgc ccatccccgc atcgacgaaa ttccatttga ttcaaaccgt
     4681 aaaatgatga cgacccttca tcgttacggc gagaaaaaaa tatcctttac caaaggcgcg
     4741 ctcgacagca tcctccgtca cacaaaatac atctggcgcg acggccgcgc cgttgaggcg
     4801 attcccgaag aggttgcaaa aataaatcag gcggcttccc ggatggcgtc gaaggcgctc
     4861 cgggttcttg cagtcagctt ctgtgagagt gatcgggccg tcgagggaga actgatattt
     4921 ctcggtctgg tcggtatgat tgatccgccc cggccggaag caaaacaggc ggtgggagtg
     4981 ctcaaagagg ccggcgttac aaccgttatg atcacaggcg accataagga aaccgcctat
     5041 gccattgccc atgatctgaa aatcgccgag ggtaaaaacc aggtcatttc aggcgacgag
     5101 ctggatcggc tgacccagga gcaattgaat gatcaagtaa aagaattaag agttttcgcg
     5161 cgggtatccc cggaaaacaa agtgatgatc gtcaacgcct tcaaaaacgg cggtaacatt
     5221 gtatccatga ccggcgacgg cgtcaacgac gctccgtcgc tcaaatcggc cgatatcggc
     5281 gtggcgatgg gcatcacggg aacagatgtc gccaaaggcg cggcggatat gattctgacc
     5341 gacgacaact ttgccaccat tgaaaaggcc attgaggaag gcaggaatat ttacaataac
     5401 atccgaaagg caacgctgta tcttctggcg tccaacttcg gcgaaatcat taccatgttt
     5461 gttgcaatcc tgatcggcct gccgtcgccg ctgagcccca ttcatattct ttgggtcaat
     5521 ctgatcacgg attctcttcc cggcctggca ctcggcgtgg atcccggaaa ccccaatatt
     5581 atgaagctca aacccagaga cccaaaagaa agtcttttcg cccggggcgg cgttttcacc
     5641 atgatattgt atggaacagt gatcggcgcg attacactgc ttggtttcca tcttggctat
     5701 caggagggcg gcctgcgcga aggccggact ttcgccctga ccattcttgc ggtttcccag
     5761 ctgtttcatg caatcggcat gcgtgacgtc gacaagtcca tctttaagat gaatcatctg
     5821 aacaaccgtt tcctgattgt gtcggttttg ctcggcctca tgctccaggt ggccatcgct
     5881 cagctgccgt atatcaattc ggtgtttggt acggttgcgc tcggttttca tcagtgggcg
     5941 attacgatac ttcttgctct ggttccgctg gcggttcatg agattgtcct gcttgtcaaa
     6001 aaagcgaccg ggaaacagat tgctgcctga taagaaagaa aaatgggaaa tctattgaca
     6061 cgcccctgct gattcgctat aataactggt aagagttaaa tgtgatgacg gagactggca
     6121 gggtcaaaac ctcttaagag agccggcgga tggtgaaagc ccggcggggt tccccgaaac
     6181 caaatcactc ccgagcaggt gcccgaaatt gcggttgcta caagcaagag taggagtaca
     6241 cgaagggccg cgttaaggca atggatttca tgcgaatcct gtaaagtgcg tttgattaac
     6301 gaatttgggt ggtaccgcgg gcatataata agctcgtccc agtattgggg cgggctttat
     6361 ttttatgtaa aattaagatg ttgctgcgcc ttccgcgggt ctgctgtaat cgtcgattga
     6421 acatcaaccg aaagacgggt gagagtgggc gagagaccat gaaaaccaaa aacgacaatc
     6481 ccgtttcccc cagactgcca aagatctggg aagattccgg cagagcggag cctgagaatt
     6541 ccggcgaaat ctcccgggcc cggataaccg accggtcgtt ccggggcttg actgcggcac
     6601 gtcttgttgt ggaaggctcg gcgtttgaga atatcgtttt tcaggactgt aaacttccgg
     6661 ctttggacgt gcaggatgcg
//
LOCUS       DATALY010000320         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_244307,
            whole genome shotgun sequence.
ACCESSION   DATALY010000320 DATALY010000000
VERSION     DATALY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244307"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VHR42_08715"
     CDS             complement(<1..691)
                     /locus_tag="VHR42_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX3027287.1"
                     /translation="MRYIPTSCLRENMVLAKSLYGSDNVLLLQKGQQLKKPYISCISN
                     CGFQGVYISDTISEDIEITNVIDDELRNSAVKAVRDIYVQSQTVGITRNMADGAKAAV
                     HSIVEEIINNRDIMVNMVDLKTYDDYTYYHSVNVGVLSIVLGVALGMNRTSLYKLGLG
                     ALLHDLGKVIIDKAIIDKEGPLTSDEFTEIKKHPGLGHEYLAHTTRLDVPMSALIGVL
                     QHHERMDGSGYP"
     gene            906..1574
                     /locus_tag="VHR42_08720"
     CDS             906..1574
                     /locus_tag="VHR42_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3027288.1"
                     /translation="MKELFSKIREEKRSRILTAATNEFAKNGFEHTNVNEIAKNAEVS
                     VGSLYKYFDSKQDIFLTTVRYCASILKTILDKVMKDDDDWQVKVEKIIRVIQVHSREN
                     KVIIQLYNEMATQSNSGIVIETVAEVESLSAELYSGLIEREQKKGGIRSDCDSRMFAF
                     FLDNLFMMLQFSYACDYYRERFKVFVSSDILQRDNFVAEQMLKFMRGAFAAEHSEEKS
                     NSRE"
     gene            complement(1728..2735)
                     /locus_tag="VHR42_08725"
     CDS             complement(1728..2735)
                     /locus_tag="VHR42_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX3027289.1"
                     /translation="MVKKLLCFTLLTAFLWSAFLLPTYAQANTPKDLVLFGDSISTGY
                     GLSDSGKSFGSLLDKALAVPSYQDLAVNGATSADVLATVKSHKEEVRTADTILLTAGG
                     NDVLNVFFSAIRSSLGLPENAPARRILESLIGKKNAVDQIESKLKDAQTQAKLKAAEA
                     GFAKNLPAIVSEIRATNPRAQLYIQTVYNPFSDGSDLFPFSGLADEILGHINTQIKSG
                     AAGKYTVIDSYSAFRNQALTLTNIAGFDIHPSEAGHAVIFRLAYEAVTGKQYAVAAAG
                     SKAPDSGSPSVAGKTSSSPSSGTAAEPYSGKLQAGAEIAGAVALVLLAWIGIRKRKRD
                     P"
BASE COUNT          700 a          655 c          727 g          740 t
ORIGIN      
        1 ccggataccc gcttccgtcc atccgttcat ggtgctgcag tacgccgatg agagcggaca
       61 tcgggacgtc cagtcgggtg gtgtgcgcca gatattcatg cccaagaccg gggtgctttt
      121 tgatttccgt gaattcatcc gaagtgagcg gtccctcttt gtcgataatt gctttatcaa
      181 taatcacttt tccaaggtca tgcagtagcg cccccagccc aagtttatag agactggttc
      241 ggttcattcc aagcgccact ccgaggacga tggaaagcac gccgacgttg acggaatgat
      301 aataagtata atcgtcgtat gttttcagat caaccatgtt gaccataata tcccggttgt
      361 tgataatttc ttccacgatg ctgtgaacgg ctgccttcgc tccgtccgcc atattccgag
      421 tgattccgac tgtctgactc tgaacataaa tgtcccgcac ggctttgacg gccgagttcc
      481 tcagttcatc atcgataacg tttgtaatct caatgtcttc cgatattgtg tcgctgatat
      541 acacgccctg aaaaccgcaa ttcgagatgc aggaaatata gggtttcttt agctgttgac
      601 ctttttgcag caaaagaaca ttgtcgcttc catagagact ttttgcgagc accatatttt
      661 cccgaagaca agatgtggga atatacctca tggatttgcc tccgcagacg aagtatttgg
      721 aaattgcatc agagtggaat tcagcccatc taattctatt attttaatct ttctgtacga
      781 tatttcaata ggggaaaagg cagaatacac caaaatgttt tatgagtcat atttttctat
      841 tataaatcta tccatttgaa cctgattgga cattttaaga ccggcgacca taaaggagat
      901 tgtatatgaa agagttattc agcaagatac gggaagaaaa acggagcaga atcctgacgg
      961 cggcgacaaa cgaatttgcc aagaacggtt ttgagcacac aaatgtcaat gagattgcca
     1021 aaaatgcgga ggtaagcgtc ggcagccttt ataaatattt cgacagcaaa caggatattt
     1081 ttcttaccac agttcggtac tgcgcgtcta ttctaaaaac gattctggac aaagtaatga
     1141 aggatgacga cgattggcaa gtcaaggtgg aaaaaatcat ccgcgttatt caagtgcatt
     1201 cccgcgaaaa caaggttatt attcagctct ataatgaaat ggcaacgcag agcaattccg
     1261 gcatcgtgat tgaaacggtg gcagaagtgg agtccctttc cgcagaactg tattccggat
     1321 taattgaacg ggaacagaaa aagggcggca ttcgctccga ctgtgacagc agaatgtttg
     1381 cattttttct ggataatctt tttatgatgc ttcagttctc ctatgcctgc gattattaca
     1441 gggaacggtt taaggtattt gtcagttcgg atattttaca gcgggacaat tttgtagcgg
     1501 agcagatgct gaaatttatg cgcggagcgt ttgctgccga acattcggag gagaaatcga
     1561 attcacggga ataaaatagc gcttgcagtg ctatgatgtc gacaaccgga gttgttcgat
     1621 caacggcggg gcgctccgca ggaccaaatt gacggcacgg tcaaatctac ccgtgagata
     1681 aaaagcacct ggctgtggct tcagttccgc ggccgggtgc ttttaactca cgggtctctt
     1741 tttcgttttc ggattccgat ccacgccagc aacacaagag cgacggctcc tgcgatttcc
     1801 gccccagcct gaagctttcc cgaatacggt tcagccgcag taccggagga cggagaagag
     1861 gacgtttttc cagcgaccga tggggacccg gagtccggag ccttcgaacc ggcggccgcg
     1921 acggcatatt gcttgcccgt gaccgcctca taggccagac ggaaaataac ggcgtgcccc
     1981 gcttcgctcg gatgaatatc gaatccggcg atgttggtga gcgtaagcgc ctggttccgg
     2041 aaggcggaat aggaatcgat gaccgtgtat ttcccagctg cgccgctttt tatttgcgtg
     2101 ttaatgtggc ccagaatttc atccgcgagg ccggaaaacg ggaaaagatc gcttccgtcg
     2161 ctaaacgggt tatagacggt ctgaatataa agctgtgcgc ggggatttgt cgcacggatt
     2221 tccgaaacaa ttgccggaag attctttgcg aagcccgcct ctgcggcttt cagttttgcc
     2281 tgcgtttggg cgtctttcag cttgctttca atttggtcca cagcgttctt tttaccgatc
     2341 aggctttcca gaattctgcg ggcaggcgcg ttttccggca ggcccagaga cgaccggatg
     2401 gcggaaaaaa agacgtttag aacgtcgttg ccccctgcgg tgagcagaat ggtgtccgcc
     2461 gtgcggactt cctctttatg gctttttacg gtagccaaca cgtctgcgga ggttgccccg
     2521 ttgacggcca ggtcttgata gctgggaacg gccagcgctt tgtcaagaag acttccgaag
     2581 cttttgccgg aatcggaaag cccgtacccg gttgagatgg aatccccgaa caaaacgagg
     2641 tctttcggtg tattggcctg cgcgtaagtc ggcaggagga aagcggacca gagaaaagcg
     2701 gtcagaagtg taaagcaaag cagctttttc accattatgg tttctccctc gcgtgaggat
     2761 attttctttt attttatcac atttcgcagg gaagggcatc ggtttgtttt gccagcggac
     2821 tt
//
LOCUS       DATALY010000321         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_68639,
            whole genome shotgun sequence.
ACCESSION   DATALY010000321 DATALY010000000
VERSION     DATALY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68639"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VHR42_08730"
     CDS             complement(<1..813)
                     /locus_tag="VHR42_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HEX3027290.1"
                     /translation="MTRVVYIDVLFVINLIFNYFILLAVSSLLHRKDKKWRLLLGACL
                     GAVYSLLIFFPSINFLYSAALKFVFSVAIVAVAYRFSTLRNLIKLIVYFYLISLLFGG
                     VIFGLYLFATPPGMLMRNGVFYVDISPVILILSSAGCYVVIHIFSRFLHRHIDTESIY
                     QIEIVVGTNGVSAAAFMDTGNTLCDPITGMPVIVAEYGTVEKLFPPMLRPAFKKGKID
                     HPDVFDAAGWKGRFRMIPFGSVGNSGGLLPAFRPDRIIVRGTGRETKISEILI"
     gene            complement(1028..2230)
                     /gene="tuf"
                     /locus_tag="VHR42_08735"
     CDS             complement(1028..2230)
                     /gene="tuf"
                     /locus_tag="VHR42_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX3027291.1"
                     /translation="MAKAKFERNKPHVNIGTIGHVDHGKTTLTAAITKVLGFKGKADF
                     MAYDMIDKAPEERERGITINTAHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHIVLARQVGVPYIVVYMNKTDQVDDPELIELVEMEIRELLTK
                     YEFPGDDVPIIKGSALVALESTSTDPNAPEYKSIVDLMEAVDKFIPTPERKADLPFLM
                     PVEDVFTITGRGTVATGRVERGLVKVGEEIEIIGLTTERKKTTVTGLEMFRKVLDFAE
                     AGDNIGALLRGVQRADIERGQVLCKPGSIHPYTKFSGQVYVLSKDEGGRHTPFFNNYR
                     PQFYFRTTDVTGVVELPEGVEMCMPGDNVSMDVELITPIAIEVGLRFAIREGGRTVGS
                     GMVTAVKD"
     gene            complement(2404..>2912)
                     /gene="fusA"
                     /locus_tag="VHR42_08740"
     CDS             complement(2404..>2912)
                     /gene="fusA"
                     /locus_tag="VHR42_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073575.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX3027292.1"
                     /translation="IVGGAIPKEFIPAVDAGIQGAMQAGVLAGYQVVDVKVTLYDGSF
                     HEVDSSDMAFKIAGSMAFKEGMKKADPVLLEPIMKVVATVPEEYMGDVIGDMNSRRGQ
                     IQGMEALAGSQQITCFVPLSEMFGYATDLRSKTQGRGQYVMEPSHYTEVPKSVQEKII
                     SSRGKKED"
BASE COUNT          822 a          728 c          629 g          733 t
ORIGIN      
        1 aataagaatt tctgaaattt tcgtttccct gcccgttccc cgaacaataa tccggtctgg
       61 cctgaacgcc ggaagaagcc ctccggaatt tcctaccgaa ccgaatggaa tcattcggaa
      121 ccttcccttc caccccgcgg catcaaaaac atcgggatga tcaatttttc cttttttaaa
      181 ggccggcctt aacattggcg gaaataattt ttcaaccgta ccgtattccg caacgatcac
      241 cggcatcccg gtgatcggat cacagagcgt atttccggta tccataaacg cagcggcaga
      301 aacaccgttt gtcccgacta caatctcaat ctgatagatg gattccgtat caatatgtcg
      361 atgtaagaat cttgaaaaaa tatgtattac gacatagcag cccgcgcttg aaagtatcaa
      421 aataaccggc gaaatatcaa cataaaatac gccgtttcgc atcagcatcc ccggtggtgt
      481 ggcaaacaga tacagaccga agatcactcc gccgaataag aggctgatca gataaaaata
      541 aacaatcaat ttgataagat ttcgtaatgt ggagaatcga taggcgacag ctacaattgc
      601 aacagagaat acaaatttca gcgccgcaga atacaaaaaa ttaatcgacg gaaaaaaaat
      661 cagcagtgaa tataccgccc caagacacgc tcccaataaa agccgccact ttttatcttt
      721 tctgtgaagc aaggaggaaa ctgccagcaa tataaaatag ttaaaaatca ggttaatgac
      781 aaaaaggacg tcgatataaa cgacgcgtgt catggaatca ccattcttcc ggcaaagcaa
      841 tctcttccac ttatctggaa gcttatcctt taacatttat tatagcgggc tcgtttggtt
      901 aagattgtcg cattgtgact gttttctgat ttttaaaatt aaaattagcc tgctgaaccg
      961 atgttatcac aaaagcaaaa aaaccgtctg ggcatagccc aaacggtttt ttgcttttga
     1021 tatttactta gtctttaact gcggtaacca ttccggaacc gaccgtacgg ccgccttcac
     1081 ggatagcaaa acgaagtccg acttcgatag cgatcggagt gatcagctca acatccatcg
     1141 agacattgtc gccaggcata cacatttcga cgccttccgg cagctctaca acaccggtaa
     1201 catcagtggt tcggaaatag aactgaggtc tgtaattgtt gaagaaagga gtatggcgtc
     1261 cgccttcgtc cttgctcagc acatagacct ggccggaaaa cttggtgtaa ggatggatcg
     1321 aaccgggttt gcagagaacc tggccgcgtt cgatgtcagc tctctgaaca ccgcgaagca
     1381 gggcgccaat gttgtcgccg gcttcagcaa agtcgagaac tttacggaac atttcaagac
     1441 ctgtaacagt cgtcttctta cgttctgtgg taagaccgat gatctcaatt tcctcgccaa
     1501 ccttgacaag tccgcgctca acacgaccgg tggcaactgt gccgcggcct gtgatggtga
     1561 agacgtcttc aacaggcatc aggaaaggaa gatcagcctt acgctcagga gtcgggataa
     1621 acttgtcgac agcttccatc aggtcgacaa tgctcttata ctccggcgcg ttaggatctg
     1681 tggaggttga ttcgagagca acaagagcgg aacccttgat gatcgggaca tcatcgcccg
     1741 ggaactcata tttggtcaga agctcacgga tttccatttc gacaagctca atcagttcgg
     1801 gatcgtcgac ttgatccgtt ttgttcatat aaacaacaat ataaggcacg ccaacctgac
     1861 gggcaagcac gatgtgctca cgggtctgag gcatcgggcc gtcggcggcg gacacgacaa
     1921 ggatcgcgcc atccatctga gcggcaccgg taatcatgtt cttgacatag tcggcatggc
     1981 cggggcagtc aacatgggca taatgcctgt tgtcagtttc atactcaaca tgagcagtgt
     2041 tgatcgtgat tccgcgctct ctctcttcag gcgctttatc aatcatgtcg taagccataa
     2101 aatcggcttt gcctttaaat ccgagtacct ttgtaatagc agcggtaaga gtagtttttc
     2161 catggtcgac gtgaccaatt gtgccgatat taacgtgtgg cttgtttctt tcaaacttag
     2221 cctttgccat tggtttgtcc tcctttataa acacacttat attctttttt actattaaat
     2281 cattctatca gcaaatgctc agaatttcaa gaggtttgca gataactata acaaatcttc
     2341 ataaaatgct tcagtttctc cccacttctt tgaaactcaa aagcaatccg ttgcaacaaa
     2401 gatttagtct tctttcttgc cgcgggagga aatgatcttt tcctgaactg atttcggaac
     2461 ttcggtataa tgactgggct ccataacata ctgtccgcgt ccctgcgttt tggaacgaag
     2521 atcggtcgcg taaccgaaca tctccgaaag cggaacgaag caagtaatct gctgtgaacc
     2581 cgccagagcc tccatgccct ggatctgtcc gcggcgggag ttcatatcgc cgataacatc
     2641 acccatgtac tcttccggaa cagtggcaac aaccttcatg atcggctcaa gcagaaccgg
     2701 atcagccttt ttcatacctt ccttgaaagc catggaaccg gcaatcttaa acgccatatc
     2761 ggaagaatcg acttcatgga atgaaccatc atacagtgtg accttaacat caacaacctg
     2821 atagccggcg agaacacccg cctgcatggc accctgaata ccagcgtcaa cagccggaat
     2881 aaattccttc ggaattgcgc ctccgacaat ac
//
LOCUS       DATALY010000322         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_413067,
            whole genome shotgun sequence.
ACCESSION   DATALY010000322 DATALY010000000
VERSION     DATALY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413067"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VHR42_08745"
     CDS             complement(<1..927)
                     /locus_tag="VHR42_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX3027293.1"
                     /translation="MSENERVIVTMLYPSIDSVKKLLKEYPTVPVFALALTDCATPVG
                     IFSLLKGRSENCFLLESVGQSERWGRYSFIGINPKAQIVIQNGKATVTCGENCRAEQI
                     DSPVEFLNRLLAEFHSPHFPGYPRLTGGFAGYFGYDMLRYLEPSLGNPPHDDLGMPDC
                     VLHRYDEMIAFDHLNGKSYAILQINNKSDVEEQYRLCNARAEELLQIISSAPPSTPAQ
                     KTRVNPTVTVNVTKQEFISRVEKAKSYIRAGEIFQVVPSRRFEVENSPDAFSVYRVLR
                     STNPSPYLYYFQAPDYQICGASPEMLVRVEGGA"
     gene            complement(1323..1622)
                     /locus_tag="VHR42_08750"
     CDS             complement(1323..1622)
                     /locus_tag="VHR42_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027294.1"
                     /translation="MFENRRVGTLTAGISLVVFGTLFLLHLVIPAISYYMISSLWPIV
                     LVLLGIEIILAYIINKNEKMHYDAGSVALIIVLSLFTVCMATAQMAIDSRWIRFY"
     gene            complement(1615..>2009)
                     /locus_tag="VHR42_08755"
     CDS             complement(1615..>2009)
                     /locus_tag="VHR42_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027295.1"
                     /translation="IFLSSWLSIGPLMYLLPVLWFYSFFDCVNLAWATAEEFSQAKDT
                     YLFQNAVFPRFSSRWALYGGILLVFFGVYMIFERLLCSFESQLNRKVFEIFYSALSVF
                     PQFFFGIVIIIIGIRLILGKKKELTDRV"
BASE COUNT          558 a          479 c          500 g          472 t
ORIGIN      
        1 ggcaccccct tccacacgga cgagcatttc cggcgatgca ccgcagatct ggtaatccgg
       61 tgcctgaaaa tagtagaggt acggagacgg atttgtggat cgcaggaccc ggtaaacgct
      121 gaaggcgtcg ggcgaatttt caacctcaaa gcggcgggac ggaacaacct gaaaaatttc
      181 tcccgcccgg atgtagctct tggctttttc tacgcgtgaa atgaattctt gttttgtcac
      241 gttgacggtt acagtcggat tcacgcgggt tttctgcgcc ggtgtggaag ggggtgccga
      301 agagatgatt tgcagcagtt cctctgctct ggcattacag agccggtatt gctcctcaac
      361 atcggatttg ttgttaatct ggagaattgc ataggatttt ccgttcaggt ggtcaaaggc
      421 aatcatctcg tcatagcggt ggaggacgca atccggcatt ccgaggtcgt catgcggagg
      481 attgccgagt gaaggctcca gataacgcag catgtcgtaa ccgaaatatc cggcaaagcc
      541 gccagtcaag cgtgggtatc ccggaaaatg tggggagtga aactcagcaa gaagacggtt
      601 caaaaattcc acagggctgt caatctgctc tgcgcggcag ttttcgccgc atgtgacggt
      661 tgctttgccg ttttgaatga cgatctgtgc tttcgggttt atgccgatga aggaatagcg
      721 gccccatcgt tccgattgcc cgacactttc cagaaggaaa cagttttcag aacggccttt
      781 caggagagaa aaaatgccca caggtgtggc gcagtccgtc agggccaagg cgaaaacggg
      841 cacggtgggg tattctttca gcagcttttt cacggaatca atcgacggat acagcatggt
      901 tacaatcacc ctttcgtttt cagacaacaa aaaaagtcca tcgtccctaa ttcctaggga
      961 cgatggactc gtggtgccac cctaattcgg gaacaaaatg ttccccttaa acaggtacac
     1021 tgcgaaactt catcggttga ttcaggtacg ccgccgttcg catgaatacc cttccgctgt
     1081 aacgcgcgga cgcggcggca gatactcgga tacctttctc tgcgctcctc acaaatccat
     1141 tcacagtcat cgtatgtgcc ggctttcacc aataccgtgc tctctttgcc accgccgtgc
     1201 ctgctacttg cttttgttct ttggatttct tcctattaac ttgttaaatt gctgcaatta
     1261 tactggaaag aatttaattt gtcaagaccc aaagttgata aatgtgaaaa aatttaaccg
     1321 tcctaataaa atctgatcca tcggctgtct attgccatct gagcggtagc catgcagaca
     1381 gtaaaaagcg aaagaacgat aatcagtgcc acagagccgg catcatagtg catcttttca
     1441 tttttgttta tgatataggc cagaattatt tcaatcccga gcagaaccag aacaataggc
     1501 caaagagacg aaatcatgta gtaagaaatt gcaggaatca cgaggtgcag aagaaagagc
     1561 gtcccgaaaa cgacaaggga gattccggct gtcagggtgc cgacgcggcg gttttcaaac
     1621 acggtcagtc aactccttct tcttcccgag aatcaggcgg attccgatta taatgataac
     1681 aatgccgaag aaaaactgcg gaaacaccga gagcgctgaa taaaatattt cgaatacctt
     1741 cctgttcagc tggctttcaa aggaacatag aagacgttca aaaatcatgt acactccgaa
     1801 aaacacaaga agaattccgc cgtaaagtgc ccagcggctt gaaaaacggg gaaacacagc
     1861 gttttgaaaa agataggtgt ccttagcctg agaaaactcc tcagcagttg cccatgcaag
     1921 gttcacacaa tcgaagaaag aatagaacca cagaaccggc agaaggtaca tcagcggacc
     1981 gatgctcagc caggaggaaa gaaagattg
//
LOCUS       DATALY010000323         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_584915,
            whole genome shotgun sequence.
ACCESSION   DATALY010000323 DATALY010000000
VERSION     DATALY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584915"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VHR42_08760"
     CDS             <1..203
                     /locus_tag="VHR42_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX3027296.1"
                     /translation="FASDPRATVLLLGMGLDEFSMSASSIPYVKNIILKHSKQEAQKI
                     LQAVMKLDSSSQIKEYLQEVQL"
     gene            200..466
                     /locus_tag="VHR42_08765"
     CDS             200..466
                     /locus_tag="VHR42_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX3027297.1"
                     /translation="MISNNYVILSPMGLHARPATELLRTIKKFKSAVTLKAGEKSSLV
                     KGILSILTLSAQSGDEISVTIEGEDENEASKALDEFFKVKIKEL"
     gene            702..2456
                     /locus_tag="VHR42_08770"
     CDS             702..2456
                     /locus_tag="VHR42_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3027298.1"
                     /translation="MEDDQKTELHRQLSRIIENEEIKIVFQPIISLRDGSVLGYEALS
                     RGPVNTPLQNPDALFGVAMDCGKLWDLELLCRTRALESAYRSGGEIKLFLNVNPYVIH
                     DEKFKRGFTKEYLENFHINPENIIFEISEKNAIGDLNGFKKTIEHYKKQNYKIAIDDA
                     GAGYSGLNMITDIHPHSIKLDLNLIHEIDKDGYKKSLVRSLYEFCRLADISLIAEGIE
                     TEGELEALIDIGVHFGQGYFIQKPEERIRPISGAVLESIKSRNAQRNHVFNHCISDIY
                     IGNLCRGNVTVSPEDLAENVYNIFLSNDDLLGVTVVLKGQVAGVVTKTRMDHVMSGQF
                     GYSLHAKRPISLIMDTCPLVTDYNMPIDKASKLAMSRSAGKLYDFIVVTRESEYCGIV
                     TIKDLLEKAMEIEVSNARHQNPLSGLPGNLIIERNLTGCIASSEPFTVLYLDLDNFKA
                     YNDVYGFENGDNVIRFVARLLHSIIPKEDFIGHVGGDDFIAILSTYDAGEICQNLMKE
                     FDAGVRTFYQAEDLNKGFIFANNRRGEEDKFPIMTISIAGVSNQRKSYQNIYQLSEHA
                     SIVKKRCKLIWESCSLID"
     gene            complement(2605..5034)
                     /locus_tag="VHR42_08775"
     CDS             complement(2605..5034)
                     /locus_tag="VHR42_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3027299.1"
                     /translation="MINERGIALRYLKSQKRRSLLMICGVVLAIALISAVFSMLNVIQ
                     EYEVAMESANGVWHVQAQDCTARQAAELSRRLDVSASGQAAYAGNAVFSQNISGALTG
                     CDENGFKQLNFKIQTGKIPVKDNEIALEAWAVKKIKPGARLGDTIEVQIKNQPTKSYI
                     LSAILKDNSKNRGSGYIRACVSLSEGKRLLKTDRTAVLMQVRNGINIEQFVKAIERDQ
                     HLEKSSVLEHSGLLAALGRSNSENVRYVYLVGGFLALLVLFSAIMMIYNSFNISVTQR
                     IRQFGMLRAMGATPKQIRRVVRSESVFVSIFGVIPGLLLGVIATTLLIAFLRITMPEY
                     FPPEGPVFYLSWLSLAIGAVTGVLATIFSSLSPARKAGRISPIEAMAASGGKSIQKKS
                     SRGFLTRVIPVETALSIRRLTTRKRSFVLTSLSLSFGILLMLCFSPVMDMLREGASHN
                     YDLGDLYIASEQESGFSQNLLQSLSRISGIQGISPQRVASVNATFPYRLLGGEYRDSI
                     KDGRWLKAKESAGGMIAAPSKSSMIGLTDAQLNKYKSKLLFGTIDPGQLNRENGVILV
                     LNYQGGLNNSDLRPGDSITVGSKALKICGVIKKDALMYLYQDSPFIGLYTTNRVFSAC
                     SNLRPNLVTMTLKRGADSDAVYKQVKSLTENIKNVQVSNQHDAKSMADKINLVGDVFI
                     YGFISVIALIGILNIINTMSTSVLVRTREIGLLRASGMTMGQITAMVASEAALYGIMS
                     LSIGLAAGIPLQRFFFILTVKSIYAVPWSMPVQLIALSSALTFAAVMLSILPPLSHIK
                     KIVITQAVTVE"
     gene            complement(5027..5716)
                     /locus_tag="VHR42_08780"
     CDS             complement(5027..5716)
                     /locus_tag="VHR42_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027300.1"
                     /translation="MQILSTRDLCKTYGKGDTSVHALKSTSISIEKGESVAIIGPSGS
                     GKSTMLHLLGGLDYPTSGTVAIDGTDIFSMKEKKLSIFRRRKIGFVFQAYNLIPVLSV
                     EENIMLPLLLDHTAPDMEYISELIRTLGLDSRRRHLPSQLSGGQQQRVAIGRALATKP
                     AIVLADEPTGNLDSKNSKEVLALLHMSVQRYNQTLIIITHNPEIASTADRVLRIEDGI
                     VNEIEAGEHHD"
     gene            complement(5818..>6246)
                     /locus_tag="VHR42_08785"
     CDS             complement(5818..>6246)
                     /locus_tag="VHR42_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3027301.1"
                     /translation="IARRSTEDFSQAAKEKNIDLAVEGDGDCEILCDPKWLQEAIGNM
                     IKNCLEYTSPGGKVTVGTEQTPVMVTIRVSDTGTGIHPDDLPYVFRRFYRGRASSGPG
                     SGIGLSLAKSIVEQMGGTLTAGGEYGRGAVFTLSFLKDVM"
BASE COUNT         1577 a         1484 c         1618 g         1567 t
ORIGIN      
        1 aattcgcatc ggacccccgc gctacggttt tgctgcttgg tatgggcttg gatgagttct
       61 ccatgagcgc ttcgtccatt ccttatgtta aaaatatcat tctcaaacac agcaaacagg
      121 aagcccaaaa aattctgcag gcagtcatga aacttgacag ttcttcgcag atcaaagaat
      181 acttacagga ggttcaatta tgatttcaaa caattatgtt attctttcac caatggggct
      241 gcatgcgagg cccgcgacag aacttctgag gaccattaaa aaattcaaat ctgctgtgac
      301 gttaaaggcc ggggaaaagt catctctggt aaaaggcatt ttgagtattc tcacgctttc
      361 cgcccagagc ggagacgaaa tttccgttac tatagagggt gaggatgaga acgaagcttc
      421 caaggcgctt gatgaatttt ttaaagtaaa aataaaagag ctttaaaagg caagcaagag
      481 ttgaaaacgg ccgtcctgtc agggcggccg tttttttgtg cctgcggccg ttccgattac
      541 ggcgattccg gttacaattg agaattgtct gatgtagaat tgctgtaaag aaagcggatc
      601 acatacaatt tacagaaata tgattttctt tatagattcg cttttgtata gtaataggtg
      661 gagatcaaaa tagaccgttc agggaagaga ggggaaaaaa gatggaggac gatcaaaaaa
      721 cggagctcca ccggcagctc agccgaatca tcgaaaatga ggagattaaa atcgtattcc
      781 agccgattat atcgctccgg gatggctccg tgcttggcta tgaggcgctg agccggggac
      841 cggtgaatac gccgctccag aatccggatg cccttttcgg tgttgccatg gactgtggaa
      901 agctgtggga cctggagctg ctctgccgta ccagggcgct cgaatcggct tatcgtagcg
      961 gcggggaaat caaactgttt ttaaatgtaa acccctatgt tattcacgat gagaaattca
     1021 aacggggttt taccaaagag tatcttgaga acttccatat aaatcccgaa aacattattt
     1081 ttgaaatttc cgaaaaaaac gcaatcggtg acctgaatgg atttaaaaaa acaattgagc
     1141 actataaaaa gcaaaattat aaaattgcga ttgatgatgc gggagccgga tattcggggc
     1201 tgaatatgat caccgacatc catccccact cgatcaaact tgatttgaat ctgatccatg
     1261 agattgataa agacggctac aagaaatctc ttgtcagaag tctttatgaa ttttgccgcc
     1321 tggcggatat ctctctgatc gcggaaggga ttgaaacgga aggggaactg gaggccctga
     1381 ttgatatcgg cgtccatttc ggtcagggtt attttattca gaagccggaa gaacggatcc
     1441 ggcctatttc cggcgcggtt ttggaatcaa tcaaaagccg caatgcccaa agaaatcatg
     1501 ttttcaacca ctgcatttcc gatatataca tcggaaatct ttgcagaggc aatgttacgg
     1561 tatctcccga agacttggcg gaaaacgttt ataatatttt tttgagcaat gatgatcttc
     1621 tgggcgtaac ggtcgtcttg aaaggacagg tggcgggggt tgtgaccaaa acccggatgg
     1681 atcacgtgat gagcggccag tttgggtata gcctccacgc caagcggccc atatcgctga
     1741 ttatggacac ctgcccgctg gtcacggatt ataacatgcc gattgataag gcgtcaaagc
     1801 ttgccatgtc tcgatccgcg gggaaactgt atgatttcat tgtggtcacg cgggaatccg
     1861 aatattgcgg tatcgttaca attaaagacc ttctggaaaa ggcaatggaa attgaggttt
     1921 ccaatgcgcg gcatcagaat ccattatccg ggcttccagg caatctgatc attgaaagaa
     1981 atcttaccgg ctgcatcgca tccagcgagc cgtttaccgt gctgtacctc gatcttgata
     2041 attttaaagc ttataatgac gtctacggtt ttgaaaacgg ggataatgtt atccggtttg
     2101 tggcgagatt gcttcattcg atcattccga aagaagattt tatcggccat gtgggcgggg
     2161 atgattttat cgccattcta tccacttatg acgccggaga aatctgtcaa aatttaatga
     2221 aagagtttga tgccggcgtc agaacatttt atcaggcgga ggatctgaat aaggggttta
     2281 ttttcgccaa taaccgaaga ggggaagaag acaaattccc gattatgacc atttcaatcg
     2341 caggtgtgtc taatcagaga aaaagctatc aaaacatcta tcagttgtcg gaacatgcga
     2401 gtatcgtgaa aaaaagatgc aaattaatct gggaaagctg cagcctgatc gactgatctt
     2461 ccgcttgacg tcaaaaccgg gctccatagg agcccggttt tgttgattct tattgaatta
     2521 ccggtaagcc gggcccgctt tgaaccgaaa gcaccccctc tgttcacgcg gagaacattg
     2581 caaaagttcg ccgtcgacca aaatttattc taccgttact gcctgtgtaa tcacaatttt
     2641 cttgatatgt gagagcgggg gaagaatgga gagcatgact gcggcgaatg tcagcgccga
     2701 tgaaagagcg atcagttgga cgggcatgct ccagggaacg gcataaatac ttttgactgt
     2761 cagaatgaaa aaaaacctct gtagcggaat cccggctgcg agaccgattg aaagcgacat
     2821 aatcccgtat agcgccgcct cgcttgccac catggctgtg atctggccca ttgtcatgcc
     2881 gcttgcccgc agaaggccga tttcacgcgt acgaaccagt acgcttgtgc tcatggtgtt
     2941 gataatattt aagattccga tcagggcgat caccgagata aagccgtaaa taaacacatc
     3001 gcccaccaga tttattttgt cggccattga tttggcatca tgctggttgg aaacctgaac
     3061 gttttttata ttctccgtca gggattttac ctgtttgtat acggcgtcac tgtcggctcc
     3121 acgcttaagg gtcattgtga caaggttcgg cctaaggttg gagcaggcgc tgaaaacccg
     3181 gttggtggta tataagccaa tgaaagggct gtcctgataa agatacatca gggcgtcttt
     3241 tttgataacc ccgcagattt tcagcgcctt ggaaccgacc gtaattgagt cgccgggccg
     3301 taagtcgctg ttgttgaggc cgccctggta gttaagaaca aggatcacgc cgttttcccg
     3361 gttcagctgg cccggatcaa ttgtgccgaa cagcagcttg cttttatact tattgagctg
     3421 ggcgtcggtc aggccgatca tcgatgattt tgagggtgct gcgatcatgc cgccggcgga
     3481 ttctttcgcc ttgagccacc ttccgtcctt aatggaatcc cggtattcgc cgccgagcag
     3541 acgataggga aaagtcgcgt tgaccgaggc gacgcgttgg ggagaaatac cctggatacc
     3601 gcttatcctg gaaagggact ggagaagatt ctgcgagaat cccgattcct gttccgatgc
     3661 aatgtagaga tcgcccaggt cataattatg tgacgcgcct tcccgaagca tatccatgac
     3721 cgggctgaag cagagcatga ggagtatccc gaaagaaagc gacagcgaag tcagaacaaa
     3781 ggagcgcttc cgggtcgtaa gccgtcggat ggaaagggcg gtttccaccg ggataacgcg
     3841 ggtcagaaaa ccgcgggaag atttcttttg aatggatttt ccgccggatg ccgccatggc
     3901 ttcgatcggt gaaatccttc ccgctttgcg ggcgggagaa agagaggaga atatggtggc
     3961 caaaacgccg gtgaccgcgc cgattgccag agagagccac gaaaggtaaa aaacagggcc
     4021 ttccggtgga aagtactcgg gcatcgtgat tcttagaaag gcaatgagga gcgttgtcgc
     4081 aatcacgccg agcagaaggc cggggatcac tccgaaaatc gagacgaaca ccgattccga
     4141 ccggaccacc ctgcgaattt gcttcggagt ggcgcccatt gcgcgcagca tgccgaactg
     4201 acggattctt tgcgttacag aaatattaaa ggaattataa atcatcatta tggccgaaaa
     4261 cagaaccaac agcgccaaaa atccgccgac aagataaacg tatcgcacat tttcgctgtt
     4321 ggaacggccc agcgcggcga gcaggccgct atgctccaga accgaactct tctcaagatg
     4381 ttgatcccgc tcaatcgctt tgacaaactg ttcgatgttt attccgtttc gaacctgcat
     4441 caatacggcg gtacgatcgg ttttcagcag gcgtttgcct tccgaaagcg agacgcaggc
     4501 cctgatatag ccacttccgc ggtttttgct gttgtctttg agaatggcgg aaagaatata
     4561 gcttttggtc ggctggtttt tgatttgcac ctcaatggtg tcgccgagcc gcgctccggg
     4621 ctttattttc tttacggccc acgcttccag agcaatttca ttgtctttga ccggtatttt
     4681 cccggtttga attttaaagt tgagctgttt gaaaccgttt tcgtcgcagc cggtcagcgc
     4741 cccgctgatg ttttgtgaaa aaacggcgtt ccccgcataa gccgcctgtc cggatgccga
     4801 tacgtccagc ctccgggaga gttccgcggc ttgccgggcg gtgcagtcct gagcctgcac
     4861 atgccagacg ccgttggcgc tctccatggc gacctcatat tcctgtatga cattgagcat
     4921 cgaaaaaaca gcgctgatca gggcaatggc caaaactacg ccgcagatca ttaaaaggct
     4981 gcgccttttt tgcgatttca gatagcgaag ggcgattcct ctttcgttaa tcatggtgct
     5041 cacccgcctc aatttcgttg acgattccgt cctcgatccg cagcacccgg tcggcagtag
     5101 atgcaatttc aggattgtgc gtaatgatta tcagcgtctg attgtaacgc tgcacgctca
     5161 tgtgaagaag ggccagcact tccttgctgt ttttgctgtc cagatttccg gttggctcgt
     5221 cggcgaggac gatcgccggt ttcgtggcca gcgctctgcc gatcgcaaca cgctgctgct
     5281 gcccgccgga aagctgtgag ggaagatggc ggcggcggct gtccagcccc aatgtgcgga
     5341 tcagttcgct gatgtattcc atgtccggcg cggtgtgatc aagcaggagg ggaagcatga
     5401 tgttttcttc caccgacaaa acaggaatca gattataggc ctgaaacaca aacccgattt
     5461 tccggcggcg gaagatagac agctttttct ctttcattga aaaaatatcg gttccgtcaa
     5521 tcgccacagt gccgctggtc gggtaatcca aaccgccgag caggtgcagc atggtggatt
     5581 ttccggaacc cgacggccct atgattgcga ccgattcccc tttctcgatc gagatgctgg
     5641 tagacttgag cgcgtggacg cttgtatcgc ctttgccata ggtcttgcaa aggtcacgtg
     5701 ttgaaagtat ctgcatgttt atgcctcctg ttttttcgtc atgccttcag tttaacagcg
     5761 caaccttaca ttacggtgaa aaaacaactt cagaacctta cagtttattt acctttccta
     5821 catgacgtct ttgagaaacg agagagtaaa caccgcgccc cggccgtatt caccccccgc
     5881 ggtgagcgtt ccacccatct gctcgacaat cgatttggca aggctcaggc cgatgccgct
     5941 tccggggccg ctgcttgcgc gcccgcggta aaacctgcgg aacacataag gaaggtcgtc
     6001 gggatgaatt cccgtaccgg tgtcgctgac ccggatggtg accatgacgg gtgtttgctc
     6061 cgtgccgacc gtgacttttc cgcccgggga agtgtattca agacaatttt taatcatgtt
     6121 gccgatggct tcctgaagcc atttcggatc gcacagaatt tcacagtcac cgtcgccctc
     6181 aaccgccaga tcaatgtttt tttctttcgc ggcctgagaa aagtcttccg ttgaccgccg
     6241 ggcgat
//
LOCUS       DATALY010000324         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_674788,
            whole genome shotgun sequence.
ACCESSION   DATALY010000324 DATALY010000000
VERSION     DATALY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674788"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHR42_08790"
     CDS             <1..529
                     /locus_tag="VHR42_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027302.1"
                     /translation="CIIALTLLITAFSATAVSCAGSGSQLKKDAAAKMARGFSCTAKI
                     SWESNNYEVKITRPADGGCTLTFVSPSELNSLCFSAGSSGAKITFGGLGISVDTSSLP
                     QYAMYKAFSACFDSCLSPDSLSTVQRGGNTGITGKNALGRFTLTFDANLNPVMLEISG
                     ANLTAHFYNFQYNQP"
     gene            708..1865
                     /locus_tag="VHR42_08795"
     CDS             708..1865
                     /locus_tag="VHR42_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3027303.1"
                     /translation="MNNFRLFNPTAILFGKDQIENLPAEIPSGSKVLITYGGGTIKRI
                     GLLDRVKDLLKDFQTGEFGGIEPNPAYETLIRAVEIVKKEGYDFLLAVGGGSVIDGTK
                     FIAAAARFEGGDPWRMLSEKLEIKSVLPLGTVLTLPATGSEMNCGAVISRKSECIKLD
                     FGSPLLYPKFSILDPVYTYTLPERQIGNGVVDTFIHVTEQYLTYPVNAKIQDRMAEGI
                     LLTLIEDGPKAITDPKDYGVRANLMWAATTALNDYIGVGVPQDWATHAIGHAITALYG
                     VDHGRTLAVILPALLNVMKAEKHDKLLQFADRVWGIRNGSPDDIIDTAIEKTAAFFRS
                     VGVPTRLSDYGIGKDAIAKIIDNLRETGHTVIGENGTITPDTVEKILTLAL"
BASE COUNT          516 a          513 c          467 g          488 t
ORIGIN      
        1 ctgtataatt gcgttaacac tgctgatcac tgcattctca gcaaccgccg tatcctgcgc
       61 gggcagcggc tcccagctga aaaaagacgc ggctgcaaag atggcccgcg gtttcagctg
      121 cacagcaaag atcagctggg aatcgaacaa ctatgaagta aaaatcacgc ggcccgccga
      181 cggcggctgc acgttgacct ttgtcagtcc cagcgaattg aattcgcttt gtttcagcgc
      241 gggcagttcc ggggcgaaaa tcactttcgg cggtttggga atttccgttg atacctcctc
      301 actgccgcaa tacgcgatgt ataaagcgtt ctccgcctgt tttgattcct gcctgagccc
      361 cgattcccta tccaccgttc agcgcggcgg caacacgggg atcaccggga aaaacgcgct
      421 gggacgattc accctgacat ttgacgccaa tctcaatcct gtgatgctgg aaatttccgg
      481 agcaaacctg acggcgcatt tttataattt tcaatacaat cagccataac tgaaaagggc
      541 tccgggcacc atgcatcatt tcagcaatta atttgagtaa taaacggctg tcatatttca
      601 ttttgtatta tttttcgact gctttttcca ccaggaacat ccggacggtt tgaccttatt
      661 cagcgtctgt gctattcttg aatcgtaaaa aagaaagggg tgttcgcatg aataactttc
      721 gtttattcaa tcctaccgca atcctgttcg gaaaagatca gattgaaaac cttccggctg
      781 aaattccgtc aggctcaaag gttttgatca catacggggg cggaacaatc aagcgaatcg
      841 gtcttcttga cagggtcaaa gatttgttga aggatttcca aacgggggaa ttcggaggaa
      901 ttgagcccaa ccctgcctat gaaaccctga tccgggcggt tgaaatcgtc aagaaggagg
      961 gctatgattt tcttcttgcc gtgggcggag gctccgtcat tgacggcact aagttcatcg
     1021 ccgcggcagc ccgttttgag ggcggcgacc cctggagaat gctttccgaa aaactggaga
     1081 tcaaatccgt ccttccgctt ggaaccgtgc tgacccttcc ggccaccggc tctgaaatga
     1141 actgcggggc ggtaatctcc agaaaatcag aatgcatcaa actggatttt ggatcgcccc
     1201 ttctttatcc aaaattctcg attcttgatc cagtctatac ttatacgctt cccgaacgtc
     1261 agatcggcaa cggcgtggtt gataccttta tccatgttac ggagcaatat cttacctatc
     1321 cggtcaacgc caagattcag gaccgtatgg ctgaaggcat tctgctgaca ttgattgaag
     1381 acggcccaaa agcaatcacc gacccgaaag actatggcgt cagggcaaat ctgatgtggg
     1441 ccgcgaccac ggcgcttaac gactacatcg gcgtcggcgt tccgcaagac tgggcaaccc
     1501 acgcgatcgg acacgccata acggctctct atggagttga ccacggaaga acactggccg
     1561 tcatcctgcc cgctcttctg aacgtgatga aagcggaaaa gcatgacaag ctccttcagt
     1621 ttgcagaccg cgtctggggc atccggaacg gttctcctga tgatatcatc gacactgcaa
     1681 ttgaaaaaac cgctgcgttc ttccggtccg tcggagttcc cacccgttta tcggattatg
     1741 gaatcggcaa agacgcgatt gctaaaatta ttgataatct gcgtgaaacc ggccataccg
     1801 taatcggtga aaacggcacc ataactcccg acacagttga aaaaatcctg accctcgcgc
     1861 tttaaaaaca taccggcaaa tgaaaatgga atggcttcgt aaaaaaagaa gccattccat
     1921 tctttatttt gtgatatttt attgaccaca atcaacatat atgctatctt tatattcaga
     1981 gaaa
//
LOCUS       DATALY010000325         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_844052,
            whole genome shotgun sequence.
ACCESSION   DATALY010000325 DATALY010000000
VERSION     DATALY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844052"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1758)
                     /locus_tag="VHR42_08800"
     CDS             complement(76..1758)
                     /locus_tag="VHR42_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027304.1"
                     /translation="METNKMKDFRSLKLLDRLRPVFEAAGVDYPVMRKILRVKLVMDG
                     RRVPTVMSNRQGSEASSGSNFSSLLLYGLIGLFIGLMVIMPIPLFVKMNIVLGMLLFM
                     IMTTMISDFSSVLLDLRDKNILLTLPVNARTLNAAKLLHIVIYLVSITVALAAASLAA
                     GLIRYGIGFFLLLLFELMLICGFVLLFTSIFYFLILRFFSGEKLKDIINYFQIILSVF
                     MTLGYQLVSRLFNFTGGNTRFSPQWWNFLLPSAWFAAPFSLLVDHDSNIFYFLLAGMG
                     IVIPILAVILYLKAVAPAFEKDLQKLAGSAEQGHTVKKHSMERTVSRFLCSGKLEKVF
                     FVFSSGIMKNERKLKLRLYPNIAFAVIFPFIFLFNFSNGRQSFAQALAEIRSGDFFLF
                     LYLSAMMLAPMLTILFMSENYKGAWIYRVLPVVSPGEILRAAFMAFVCKYVFPVFFFS
                     GLLFTLIYGPQIIPDLILIFFNALLLMLILFKYSGKQLPFCRDFQSTQNNNMTIVILS
                     LVLCGVFAGLHFLLRRLFTPGIFLFLVVSAVFSLLLWRTAFHVTWQELEGND"
     gene            complement(1763..2548)
                     /locus_tag="VHR42_08805"
     CDS             complement(1763..2548)
                     /locus_tag="VHR42_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027305.1"
                     /translation="MKPTGEPVISIKDLRMSYGSHEVLKGINLEIYQGQIIGYIGPNG
                     AGKSTTVKILLGILEGYQGDVKVLGVDLTSGSIEYKKRIGYVPETADIYESLTAQEYL
                     TFIGELYGIDYDTADSKANRLMALFGIGEGYTARISSFSKGMKQKVLIIASLLHDPDI
                     LFFDEPLSGLDANSVMVFKEVMSELAAQGKTIFYSSHIMEVVEKISDRIIILSGGHIM
                     ADGTFEELKEQCREGSLEEIFTQLTGFDRQRDIAGEFISILKE"
     gene            complement(2851..3789)
                     /locus_tag="VHR42_08810"
     CDS             complement(2851..3789)
                     /locus_tag="VHR42_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3027306.1"
                     /translation="MIELFTITCIAAAVLGIRLFQIKRQLHHITVQLDERTGGKTEKK
                     ITVSLIDGNVNELAAAMNRSLNVQKELRTEVWRKDRQLKDSIANLSHDLRTPLTSILG
                     YLQLFRSPDCPAEKKDDYLRIISEKAHVLKTLINGLYELSVLDVRHLKEEELDLNLLL
                     SDVLAGQYELFEKFEISLRVNLPDSPVRIFGDRVACTRILQNLLNNTIRYAKESAEIV
                     LEKRDSCAVLSICNSAPNLTQEDTEHLFDRFYTADQSRNEGGTGLGLSIVKTLLLQMG
                     GTVDASLRDRKLCLTIQLPLLANTQHGMSAKPKLDQ"
     gene            complement(3812..4591)
                     /locus_tag="VHR42_08815"
     CDS             complement(3812..4591)
                     /locus_tag="VHR42_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3027307.1"
                     /translation="MSNLIKYRWFELRHNWILRLMFLICVVFAWVLISPHYLTDSPMV
                     PGIPDNMQGLFMASAADCIFPLLIISGAFTAMMLGRQFSDRTIDLEIVAGHSRAAIFA
                     VQCLVAFVIPGATVLLAILIGCFRWAGIVPISGTAFLFFIRAILLLLLLSFSLVSACI
                     LFAVLFRDTAKTMAVSAFFLLIACWTMPAMEQSLIKAPGTLYPASPSLALLLHPAFLM
                     RYVLYPALTPGQGIWVTVIAASWSLLFLGTAYCIFRHCELK"
     gene            complement(4604..5314)
                     /locus_tag="VHR42_08820"
     CDS             complement(4604..5314)
                     /locus_tag="VHR42_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027308.1"
                     /translation="MGRLLHAEFYKLVHDKIYWITFTAILLFNMILLSGSSVLSFTGQ
                     EALAESMEKEIVTILIVCIYEGLFIGDDFAGRTLYHSLSAGKSRSSVLFAKSAVSLIA
                     ADGLLFFFPLLLILTCTVKNGWGASAFVQPQRMISMTIAFLILGFAVGGISLLAAVCF
                     RDVGRTIGIPILLYFFMILLLNGSASPVFSRILPVGILVLLANGSVSPYYGILIGTLW
                     IAVLEAVSILIFRRSELR"
     gene            complement(5316..>6193)
                     /locus_tag="VHR42_08825"
     CDS             complement(5316..>6193)
                     /locus_tag="VHR42_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027309.1"
                     /translation="KYRGTLALDHINLTLRRGEIYGFIGENGAGKTTTIRLITGLSFP
                     TEGRLSLFGTSDEKQLQKQRERMGCMVETPALYKNMTAEQNLEAQRIQRGIPDRECIK
                     ETLKLVGLSDTGKKAARHFSLGMRQRLGIAMALLNDPEFLILDEPINGLDPSGIVEIR
                     ELMKRLNQEKGITILVSSHNLGELYQTATRYILLHKGRLLEELSQKELEEKCKRHIAI
                     QTNDSAKTAAALENLLKTTNFQVMPDGTVRLYDHLDETENVARILSQAGLLVTGFSLA
                     GDSLEDYFLHRIGGR"
BASE COUNT         1668 a         1537 c         1538 g         1450 t
ORIGIN      
        1 ttccgaatag ttgcttcatt ggggggctgg ggcgggctgc cgcccgttgt gaatcaggtt
       61 tcgcggtacg gccttttagt cgtttccttc aagttcctgc catgtgacat gaaaagcagt
      121 acgccataaa agtaagctga acacagccga caccacgagg aagagaaaga ttcctggggt
      181 gaaaagccgc cttagcagaa aatgtaggcc cgcaaacacc ccgcataaga ccaaagaaag
      241 aattacgatg gtcatattgt tattctgcgt ggactgaaaa tccctgcaga acggaagttg
      301 cttgccggag tatttgaaga gaatcagcat cagaagcaac gcgttgaaaa agatcaaaat
      361 aaggtcggga atgatttggg gcccgtagat caacgtgaaa agaaggccgg agaaaaagaa
      421 gaccgggaac acgtatttgc agacaaaggc cataaaggct gctcggagta tttctccagg
      481 actgacgacg ggcaaaaccc ggtaaatcca tgcacctttg tagttttcgc tcatgaaaag
      541 gattgtgagc atcggagcga gcatcattgc ggacaggtat aggaataaaa agaaatcacc
      601 gcttctgatt tctgccaatg cctgagcaaa tgattgccga ccgttcgaaa aattaaaaag
      661 aaagataaac gggaagatta ccgcgaacgc gatgttcggg taaagcctga gcttgagttt
      721 tcgttcgttt ttcataatcc cagaagaaaa aacaaaaaac accttttcca gcttgcccga
      781 gcagaggaaa cgtgaaaccg tgcgttccat tgagtgcttt ttcaccgtgt ggccctgttc
      841 cgcactgcct gcaagctttt gaagatcctt ttcaaaggca ggcgctacgg ctttgagata
      901 gaggatgacc gccagaatcg ggatgacgat ccccatccca gcgagtagaa agtagaaaat
      961 gttggaatcg tgatccacca acagactaaa gggtgcggca aaccacgccg agggcagcag
     1021 aaaattccac cattgggggg agaaacgcgt gtttccgccg gtaaaattga acagccggct
     1081 taccagttgg taacccagag tcatgaagac ggaaaggatg atctgaaaat agttgatgat
     1141 gtccttgagc ttttctccgc tgaaaaagcg aagaatgagg aaatagaaaa tcgaggtgaa
     1201 caaaagaaca aatccgcaga tcagcatcag ctcaaacaga agcagcagga agaaccctat
     1261 tccgtagcgg atcagcccgg ccgcaagcga ggccgcggcc agcgcaaccg ttatgctgac
     1321 cagataaatg acaatatgca ggagctttgc cgcgttgagc gtgcgcgcgt ttaccggcag
     1381 agtgagcaga atgtttttat cccttaaatc cagcagtacg gaagaaaaat ctgaaatcat
     1441 cgtggtcatg atcatgaaca gaagcatacc caatacgatg ttcattttta cgaacagcgg
     1501 aatcggcata atcaccatta gcccgataaa cagaccgatc aggccgtaga gaagcagaga
     1561 agagaagttg gagccgctgc tcgcttcgct gccctgccgg ttgctcataa cggtaggtac
     1621 tctgcggccg tccatcacca gcttgacacg aagaattttg cgcatgacgg gatagtcgac
     1681 cccggcggcc tcgaaaacag gacgcagccg gtcaagaagc ttaagcgacc gaaaatcctt
     1741 catcttattt gtttccatcc catcactctt tcaaaatgga gataaactca ccggcgatat
     1801 ctctctgccg gtcgaaaccg gtgagctggg tgaaaatttc ctcaagcgag ccttcacggc
     1861 actgctcctt gagctcttca aaggtgccgt cggccatgat gtgaccgccg ctcagaataa
     1921 tgatccggtc gctgatcttt tcgaccactt ccatgatgtg ggaggaataa aaaatcgttt
     1981 tgccctgagc cgcaagctct gacataactt ctttaaacac cattacgctg ttcgcgtcca
     2041 atccgctcag aggttcgtcg aaaaagagaa tatccgggtc gtgcaaaagg ctcgcgatga
     2101 tcaggacctt ttgcttcatg cccttggaaa acgaggatat cctcgcagtg taaccttcgc
     2161 cgatgccgaa cagtgccatc aggcgatttg ctttgctgtc ggcggtgtcg taatctatgc
     2221 cgtacagttc accgatgaag gtgaggtatt cctgtgcggt gaggctttcg tatatgtcgg
     2281 ccgtttccgg cacgtagccg attctttttt tatattcgat gctgccggaa gtcagatcta
     2341 ctcctaaaac cttcacgtcg ccctgataac cctccagaat gccaagcaaa atttttaccg
     2401 ttgtgctttt gcccgcgccg ttgggcccga tgtagccgat gatctgcccc tggtatattt
     2461 caagattgat gcctttcagc acctcatggc tgccgtagct cattctcagg tcctttatgg
     2521 aaatcaccgg ttcgcctgtc ggcttcatca cgtttccctc ccgctttcaa gttttggctt
     2581 taaaccttat agcattttaa aatggacaaa tgctgcccga atatcagctt gttttttaag
     2641 aggacacgga tgcgtcctct tgtcagcgga atatcgattc ggcgatattc tgggatgtag
     2701 accgcaaagt tctttcatgt gagcaatcca gcaggatttt ggcgtatacc cataagtcat
     2761 tcgaataaac ttctgcaatt ccttataagt agccattaaa cttggccccc tcaaattaac
     2821 agcgaagtgt gattccattt tccacatggc tcattggtcc aattttggct ttgcactcat
     2881 tccatgctgc gtatttgcta ataacggaag ctgaattgtt aagcaaagtt tccggtccct
     2941 taaagaggcg tcaacggttc cacccatttg aagcaaaagg gttttcacaa tcgaaagccc
     3001 caaacctgtg ccgccttcat ttctggattg atcagccgta tagaagcggt caaataggtg
     3061 ctccgtgtct tcctgcgtca gattcggtgc ggaattgcag atcgacagga ctgcgcagga
     3121 gtcccgtttt tccagaacaa tttcggcact ttctttcgca tatcggatcg tgttattcag
     3181 taagttctgc agaatgcggg tgcatgcgac gcggtcgccg aaaattcgga cgggggaatc
     3241 ggggagattt acacgaagcg agatttcgaa tttctcaaac agttcatatt gtccggccag
     3301 cacatcggaa agaagaagat tcaaatccag ttcttcttct ttgagatgac gaacatccaa
     3361 taccgacaat tcataaagac cattaatcag ggttttcagc acatgagctt tctcgctgat
     3421 gatccggaga taatcgtcct ttttttccgc gggacagtcc gggctccgaa acaattgaag
     3481 atatcctaaa atcgaggtga gcggggtgcg aaggtcgtgg ctgaggtttg cgatggaatc
     3541 tttcagctgg cggtctttcc tccaaacctc cgttcggagt tccttctgta cgttcagact
     3601 gcggttcatg gcggcagcaa gctcgtttac attcccgtcg atcagcgaaa ctgtaatttt
     3661 tttctcggtt tttccaccgg tgcgttcatc aagctgaacc gttatatggt gcagttgccg
     3721 cttgatttga aaaaggcgga taccgagtac ggcggccgca atgcatgtga tcgtgaacag
     3781 ttctatcatt gcagcctccc tctttcccgt actattttaa ttcacaatgg cgaaatatac
     3841 aataggcggt gcccaaaaac agaagagacc agcttgccgc aataacggtt acccagattc
     3901 cctgcccggg ggtgagggcg ggatataaaa cataccgcat cagaaaggcc gggtgcagca
     3961 ataacgcaag cgacggtgag gcagggtata gggtcccggg cgctttaatg agcgactgct
     4021 ccatggccgg catcgtccag cacgcgatca agaggaaaaa agcagaaacc gccattgttt
     4081 ttgcggtatc ccgaaagaga acggcaaaca gaatgcaggc ggagacaaga gaaaaactga
     4141 gcagcagcaa cagcaggata gcgcgtataa aaaacaaaaa tgccgtgccc gagatgggaa
     4201 cgattccggc ccaacgaaag cagccgataa gaattgccag caatactgtt gcgccgggaa
     4261 ttacaaatgc cacaaggcac tgcaccgcaa aaatagccgc ccgactgtgc cctgcgacaa
     4321 tttccagatc gattgtccgg tcggagaact ggcggccgag catcatggcg gtaaaggcgc
     4381 cgctgataat caataagggg aagatgcagt ccgccgccga agccataaag agcccctgca
     4441 tgttatctgg tattccgggg accatgggcg aatcggtcag ataatgcgga ctgatcagaa
     4501 cccatgcgaa tacgacgcag atcaggaaca tcagccgcaa aatccaatta tgtcttaatt
     4561 caaaccatct gtatttgatc agattactca tttaattctc tccttatcgc agctctgacc
     4621 ggcggaaaat cagaatggaa acagcctcca gtacagcaat ccacaaagtt ccgatcagga
     4681 ttccataata gggggagacg gacccgtttg ccagcagcac cagaattcct accggaagaa
     4741 tacgggaaaa aacaggggaa gcagagccgt tcagcaacag aatcataaaa aagtacagga
     4801 ggatcggaat cccgatcgtg cggccgacat cccggaaaca cacggccgcc agaagagaaa
     4861 tgccgccgac agcaaatccg agaatcagga aggcaatagt catggaaatc atcctttgcg
     4921 gctgcacgaa agccgatgcg ccccatccgt ttttgacggt acacgtcaga atcaacagaa
     4981 gagggaagaa gaacagcagc ccgtcggcgg caatcagcga tacagcggac tttgcaaata
     5041 aaacggaact ccggcttttc ccggcggaca ggctatggta cagcgtccgg cctgcaaaat
     5101 catcgccgat aaacagaccc tcgtaaatac agacaatcaa gatggtcacg atttcttttt
     5161 ccatactttc cgccaatgcc tcctgcccgg taaaactcag gacggaactg ccggaaagta
     5221 gaatcatatt gaataataga atggcagtaa acgtaatcca gtaaattttg tcatggacaa
     5281 gcttatagaa ttccgcatgt aacagtcgcc ccatgttacc ggcccccgat tctgtgcagg
     5341 aagtagtcct ccagactgtc tccggcaaga ctgaagcccg tcacaagcag acctgcctga
     5401 gaaagtatgc gtgctacgtt ctccgtttca tccagatggt cgtatagccg tacagtgccg
     5461 tccggcataa cctgaaaatt cgtcgttttc aggaggtttt ccagcgcggc cgcggtttta
     5521 gcggaatcgt tggtttgaat cgcaatatgt cgtttgcatt tttcctccag ttccttttgc
     5581 gacagctctt ccagcaggcg gcccttgtgg agcaaaatgt atcgggttgc cgtttgatac
     5641 agttcaccga ggttgtggct ggagacaaga atcgtaatgc ctttttcctg attcagccgc
     5701 ttcatcagct cccgtatttc cacaattccg gagggatcaa gaccgttgat cggttcgtcc
     5761 agaattaaaa actccgggtc gttcagaagc gccatcgcaa ttccgagccg ctgacgcatg
     5821 ccgagagaaa agtggcgcgc ggcctttttt ccggtatccg ataatcccac cagcttcagt
     5881 gtttctttga tgcactcccg atcgggaatg ccgcgctgaa tacgctgagc ttccaggttc
     5941 tgctcagcag tcatgttttt atacagcgcg ggtgtttcaa ccatacatcc catgcgttcc
     6001 cgttgctttt gcagctgctt ttcatcggat gttccaaaca gcgacagacg cccctccgtc
     6061 ggaaacgaca gtccggtaat gagacggatc gttgttgttt tgcccgcgcc gttttctccg
     6121 ataaaaccat aaatttcgcc gcgccgcagg gtaagattta tatggtcaag cgccagagtg
     6181 ccgcggtatt ttt
//
LOCUS       DATALY010000326         3984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_245970,
            whole genome shotgun sequence.
ACCESSION   DATALY010000326 DATALY010000000
VERSION     DATALY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245970"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VHR42_08830"
     CDS             <1..1413
                     /locus_tag="VHR42_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HEX3027310.1"
                     /translation="AYADQQDSRDYTSPAGAGTVPVYIGRAPVHQLADYKNKTNLPIL
                     ISSWQDGYKKIGYSEDWKNFDLCEAVYAHFRNAVNSVGPIVVINVLNPDDKKNETQQT
                     VTAKFIKRTATISNSKVILSTISIAQKTLGTDYSAEYSDDGLSVILKDLKGGLDSVSV
                     SFYEVDPSAVTDTDVVTGINTGVPRVYYDLSDIPTILCAPGWSQKITVFAAMVAMQDK
                     INGHWYAWVNADLDTSAAKKVDDAITAKAGYDTETGAGALLWPMAKKGNRIFHASTLE
                     TVTMQWVDYKNNNVPYESPSNKQIDIDSLCLGDGSAVYFDQTEANRLNQNGIDTMTFW
                     EGVWRMWGPHTMKYVEGTSIDARDVFDCSVRMMRYIENTFQRRYGVEVDKPMTRGKKD
                     SILNDFQAWLDGLIQEGALMLASVEFQEADNPSSDLVEGNFVFSTEFTNPVPGKSISN
                     KVRWTSEGLNSLFGDDLSGS"
     gene            1403..1918
                     /locus_tag="VHR42_08835"
     CDS             1403..1918
                     /locus_tag="VHR42_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HEX3027311.1"
                     /translation="MGRKLIVGAVGGIKILANGNEISNVTSISLPEIEQKGEEVSGGG
                     ILGTFSLPTPGHFSAMNTTVSMRAAGQDKKYLLTTTVNLEIRLAANMRASDGTMYVSG
                     TRIYIIGHPLKIGNGSGEIGKTKEESYEYSTTRYREVVDGEETILIDQVASVYKVGGV
                     DMLSGLHAALD"
     gene            1934..2383
                     /locus_tag="VHR42_08840"
     CDS             1934..2383
                     /locus_tag="VHR42_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027312.1"
                     /translation="MDNIENEKTGMGDLSDGRTLIPMGELGAEDTADSEELQDSDHVV
                     VLKKPIKIAGKEYKHLEFDFDELTAKDLHQASKYLKNLGIPVSVPALDFEYQLTLFVR
                     AVKLKMPDVEFADLMRLSAADASKATGLVRDFLLDKDPGLKDLGSDE"
     gene            complement(3122..>3984)
                     /locus_tag="VHR42_08845"
     CDS             complement(3122..>3984)
                     /locus_tag="VHR42_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027313.1"
                     /translation="AYAMSKGMKTNYQDMSQAQQATLRYNYLLNATKDAQGDFAKTSG
                     SFANQQRLLKSNLQQVAGTIMSKMLPALGQGLQKVNAFVLGLDTNKVGAFVGQIGNMA
                     LSFLPLVMQLLPMFGSLLQMVIPPLINIGQQIMPIVIQVVRTVMGVLQPLIPPVMQFA
                     QALLPPIQQMLTAVSPLLITLANIISKFIGPALSFVIGIITTLANLITSVAKPIGDFF
                     NSIGSFLGIGGQQKATTATAKTASVPKYAEGGFADSPSIFGEAGLEAAIPIRPGSARI
                     FSNASLGSKL"
BASE COUNT         1073 a          902 c         1067 g          942 t
ORIGIN      
        1 gcttatgctg atcagcagga cagccgggac tatacttcgc ctgccggtgc cggcacggtt
       61 cccgtctata ttgggcgcgc gcctgttcat cagctggcgg actataaaaa caaaaccaat
      121 cttccgattc tcatttcatc ctggcaggat ggatataaaa aaatcggtta cagcgaagac
      181 tggaagaatt tcgacttgtg cgaagcggtt tacgcgcatt ttcgcaatgc agtaaacagc
      241 gtggggccga ttgttgtgat caacgttctc aatcccgatg ataaaaagaa cgaaacgcag
      301 cagacggtta cggcaaaatt cattaagcgc acggcgacga tttcaaattc aaaggttatt
      361 ctttcgacta tatccattgc tcagaaaacg cttggcacgg attacagcgc ggagtactcg
      421 gatgacggac taagcgtgat ccttaaggat ctgaagggcg gtttggacag tgtttcggtg
      481 tccttctatg aagtggatcc ttccgcggta acggatacgg acgttgtgac cggaatcaac
      541 acgggagttc ctagggttta ctatgatctg tcggatatcc ccacaattct gtgcgcccct
      601 ggctggagcc agaagataac tgtctttgcc gccatggttg cgatgcagga caaaatcaac
      661 ggtcactggt atgcgtgggt taacgcggat ctggatacat ccgctgccaa aaaagtcgat
      721 gatgcaatca cggcgaaggc gggttacgat accgaaaccg gagcaggtgc gcttctgtgg
      781 ccgatggcga aaaagggcaa tcggattttc catgccagta cgctggaaac ggtcacgatg
      841 cagtgggtcg actacaaaaa caacaatgtt ccgtatgaat cgccgtccaa caagcagatc
      901 gatattgatt ctctctgtct gggagatggc agcgcagttt atttcgacca gacggaagca
      961 aaccggctga atcagaacgg gattgatacc atgacgttct gggaaggcgt gtggcgaatg
     1021 tgggggcctc ataccatgaa atatgttgag ggaaccagca ttgatgcccg ggatgtattc
     1081 gactgctccg tccgaatgat gcggtatatt gaaaacacct tccagcgccg gtatggcgtt
     1141 gaggtcgata agccgatgac acgcgggaaa aaagattcca tcctgaatga ttttcaggcg
     1201 tggctcgacg gcctgattca ggaaggtgct ctgatgcttg cttccgtgga gtttcaggaa
     1261 gcggataacc catcaagcga tctggtcgaa gggaactttg tgttctccac cgaatttacg
     1321 aatccggtac caggcaagag tatcagcaac aaggttcgct ggacttcgga aggccttaat
     1381 tctttgtttg gagatgattt aagtgggtcg taagttaatt gtcggcgctg ttggcggtat
     1441 taagatattg gcgaacggaa atgaaatctc caatgtaacg agcatttccc tcccggagat
     1501 tgagcaaaaa ggtgaggaag tttccggcgg cggaattctt ggcacattca gcctgccgac
     1561 gcccggtcat ttcagcgcaa tgaacacgac cgtttccatg cgcgcggccg ggcaggataa
     1621 aaaatacctg cttacaacaa ccgtgaatct ggagattcgg cttgcggcca atatgcgcgc
     1681 aagtgacgga acgatgtacg tttccggaac gcggatttat attatcggtc atcccctgaa
     1741 aatcggaaac ggtagcggcg agatcggcaa aaccaaagaa gaaagttacg aatactccac
     1801 gacccggtac cgcgaggtag ttgacggtga ggaaacgatc ctgattgacc aggtcgctag
     1861 tgtttacaag gtcggcgggg tcgacatgct cagcggcctg cacgccgctc tggattaaga
     1921 aaggcggaac gaaatggaca atattgaaaa cgaaaagaca ggtatgggcg atctttcgga
     1981 cgggcgtaca ctgattccaa tgggcgagct tggtgcggaa gatacggcag attcagagga
     2041 actgcaggat tccgaccatg tggttgtttt gaaaaagccg atcaaaatcg cgggtaaaga
     2101 gtataaacat ctggaatttg attttgatga actgactgcg aaagatcttc atcaagcgtc
     2161 aaaatacctg aaaaatcttg gtattccggt cagtgttccg gcgctggact tcgaatatca
     2221 gctgacgctt tttgtccgcg cggtgaagtt gaaaatgccg gatgtggagt ttgccgatct
     2281 gatgcggctg tccgctgccg acgcttcgaa agcaaccggt ttggtaaggg attttttact
     2341 cgacaaagat cccggactca aggacttggg atccgacgaa taatcgccca gttgacgatg
     2401 accacggcaa acgatgtaaa tgtgctggaa aaccttcctt tagatgatct gttttatctt
     2461 tatgaggatg taagtgctga attatcgaaa gccgcggctc ccctgaagga cggtgaggag
     2521 cagtgaaggg gaaagaatta agagcgctca ttactcttgc ggggaaggtt gatccgtccc
     2581 tgcaggcggc aatgctgaaa gcaagcaacc aaacgaaaaa gacgtctaat gtctttcaaa
     2641 aagcgcttgg cgcaatgaac aaaggtgttt tgaaggtagc cggaaaaatc agtccggcat
     2701 ttcaaagcca gcttcaaaag gtagaatcaa aagcctcaaa agcctttagc tctgttcagc
     2761 agcatgctgg acggagcgaa aaaggaatgt tgactttagg aaaagctgtg aaagccattg
     2821 ccggtggttt tgtcgtagga aaagtaattt caggcctgtc gtcgatggca agtaagggac
     2881 ttgaatatgc gagcagcctg aatgaagtgc agaatgtggt tgatacgacg ttcggcaaaa
     2941 gcgcttctgt ggtaaatcaa tttgcaaaaa ctacattaaa gtcatacggg ctttcagaac
     3001 tgcaggcaaa gcaatatact tccactcttg gttccatgta taaaagcatg ggcctcagtt
     3061 ctcagcaaac actgacgatg tcccagaaca tgaccgcact gtccggcgat atggcttcgt
     3121 tctacaactt ggatccgagc gaagcatttg aaaaaatccg ggcgctgccc ggccggatcg
     3181 gaattgccgc ttccaggcct gcttcgccga aaattgacgg gctgtccgca aaaccgccct
     3241 ctgcatattt tggaacgctt gccgttttcg ccgttgctgt cgttgctttc tgctgcccac
     3301 ctattccaag gaatgaaccg attgaattaa agaaatcgcc gatcggtttt gcaacgctgg
     3361 taattaggtt tgccagagtc gtgatgattc cgataacaaa ggacaacgcc ggacctataa
     3421 acttgctgat gatattggcc agcgtgatca aaagcggcga aaccgcggta agcatttgct
     3481 gaatcggcgg caaaagagcc tgagcaaact gcataacagg cggaatcaga ggctgaagga
     3541 ctcccattac cgtccggaca acctgaatca caattggcat gatctgctga ccgatattga
     3601 tcagcggtgg aataaccatc tgaagcaggc tgccgaacat cggcagcagc tgcatgacca
     3661 aaggaaggaa ggataaagcc atatttccaa tttgtccgac aaaagcgcca accttattcg
     3721 tatcaagtcc cagaacgaaa gcgttaacct tttgtaggcc ctgccccagc gcaggaagca
     3781 tttttgacat aattgttccg gcaacctgct gcaaattact ttttagcagc ctttgctggt
     3841 ttgcgaagct tccgcttgtt tttgcaaagt caccctgagc gtcttttgta gcattcaaaa
     3901 ggtaattata gcgaagcgtt gcctgctggg cctgcgacat atcctggtaa ttggtcttca
     3961 tcccctttga cattgcgtaa gcct
//
LOCUS       DATALY010000327         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_675251,
            whole genome shotgun sequence.
ACCESSION   DATALY010000327 DATALY010000000
VERSION     DATALY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675251"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1751)
                     /locus_tag="VHR42_08850"
     CDS             complement(60..1751)
                     /locus_tag="VHR42_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX3027314.1"
                     /translation="MAAEKTVDIFSGAKVQKRKKHIAVWVSLSALLILGALGGVFGKQ
                     FYQEYQRQKQISSVIDVDTFYKGIVVQGIDLGGKTMDEAKAEIKQLEPGLRGKYDIKI
                     QYKGKTWNITENDLAFTFNTDSVLQEAYSYARSGDRTERYNQVLALEQTPKNYEIANS
                     MSYENINAKLKEMVKGIAYAPVDATVASFNSATETFQYADGKDGLAVDENKLYQQVVG
                     LINGEKTGTIQVPTKAVPYSKTIAEISSHLQKLGTYSTVSTNNANGTHNMKLALSKVN
                     GTCIPAGGTFSFNKIVGDSSKAANGFLEAGAILNGKFIKAYGGGICQASTTIYGAALR
                     SNMKIVQRSNHTIQSTYCPIGQDAAVSYPELDLQFVNSTEYPVYIVTGSKGKVLTATF
                     YGYQSPDYDKIEITSVKTASIPAPTAPKYTVDKTLAKGVIKLDGKARDGSRATAQRVF
                     YKNGAVVKTENLPASYYHALPAFYSIGPGTPVPSAPPSSASSQASSSAASSAASKPSS
                     SSSSKPESSAPPASSSQPSSPASSAASSEQPGSSPSPSSDTPSDDPLNNNEVMPD"
     gene            1971..3095
                     /locus_tag="VHR42_08855"
     CDS             1971..3095
                     /locus_tag="VHR42_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3027315.1"
                     /translation="MKILILSAATGGGHLRASHAIESYIRENSTGNEVAVVDALKSIN
                     TVLDKTVCEGYHFLATKTPKVFGQLYRKTNEESILSNLVTRFTSVFSQKLIPLMEEQK
                     PDVVISTHPFATEMVSHLKGKGIVTAPLICLMTDYGPHRAWFGDHVDAYIVSTADMIP
                     EMEAMGVKKEKIYPFGIPVGDVFFTTKEKPELLEKFGMDAGKPTILIMAGSFGVTNIM
                     NIYKDIIRVPADFQIIVITGRNQKLFEAFTPVVENSPKKTKLVFFTNEVENYMHASDL
                     IITKPGGLTVSEALACNIPLAVFDAIPGQEEDNANFLLTHDMAVKLEKGVDTGKTIQT
                     LLEDSRRLEEMRSSCEEFDTSESSKNIFSLINELCEKNIK"
     gene            complement(3170..3634)
                     /locus_tag="VHR42_08860"
     CDS             complement(3170..3634)
                     /locus_tag="VHR42_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3027316.1"
                     /translation="MKFSAPLISAADFSVSRKFYEEVFGLKVICDFGANITFEGGFSL
                     QTKKTWAEFIGKSEEEILTKSNNFELYFEEEDFDGFLEHLKKFPEIELVHGVKTFPWG
                     QRGIRFYDPDKHILEVGETMTAVIKRFLKQGLSVEETSKITMMPMEYVKSCL"
BASE COUNT          901 a          948 c          947 g          966 t
ORIGIN      
        1 cagatgcaaa attttccttc tgaacctgag cggcgcggcg cacgatcatt cctaattttc
       61 taatcgggca taacttcgtt gttgttcagc gggtcgtccg agggagtatc actggaaggg
      121 gatgggctgc tgcccggttg ctcggaggat gctgcggagg aagcggggga cgacggctgg
      181 gaagaactcg caggcggggc agagctttcc ggtttggagg agcttgagga agacggtttg
      241 gaagccgcgg acgaggccgc cgatgaggac gcctgcgacg aggcggaaga cggcggagcg
      301 gaaggcaccg gcgttcccgg gcctatggaa taaaacgcag gaagtgcatg ataataggaa
      361 gcgggaaggt tctccgtttt cacgaccgcg ccgttcttgt aaaaaacacg ctgggcggtc
      421 gcccttgagc cgtcccgcgc cttgccgtcc agcttgatga cacccttggc gagcgttttg
      481 tccacggtgt atttcggcgc ggtcggggcg ggaatcgaag ccgttttgac ggaagtgatt
      541 tcaattttat cgtaatccgg cgactggtag ccgtaaaagg ttgcggtaag tacttttcct
      601 ttgctgccgg taacaatgta aaccggatat tcggtggagt ttacaaattg caggtcaagc
      661 tccgggtaac tcaccgcagc atcctgcccg atggggcagt aggtggactg tatggtgtgg
      721 ttggaacgct gcacgatctt catattggag cgcagcgccg cgccgtaaat cgtagtggaa
      781 gcctggcaga tccctccgcc gtaggcttta atgaattttc cgttcaaaat cgcgcccgct
      841 tccagaaatc cgttcgctgc tttggaggag tcccccacga tcttgttaaa agagaaggtt
      901 cctcccgccg ggatgcaggt tccgttgacc ttggagagcg caagcttcat attatgggta
      961 ccgttcgcgt tgtttgtgga cactgtgctg taggttccca gcttctgtaa atggctgctg
     1021 atttcggcga tcgtcttgga atacggaacc gctttggttg gcacctgtat ggtgccggtc
     1081 ttttccccgt tgatcagccc gaccacctgc tggtaaagct tgttttcgtc taccgcaaga
     1141 ccgtccttgc cgtctgcgta ctggaaggtc tccgtcgctg aattgaagga ggcaacggtc
     1201 gcgtccaccg gggcatacgc aatgccctta accatttctt tcagtttggc gttgatattc
     1261 tcgtaactca tggaatttgc gatctcatag ttttttgggg tttgttcaag ggcaagcacc
     1321 tgattgtagc gttccgtccg gtcacccgac cgcgcgtagg aataggcctc ctgcagaacg
     1381 gaatccgtgt tgaaggtgaa cgcaaggtcg ttttccgtta tgttccaggt tttgccctta
     1441 tactgaattt taatgtcata ttttccgcgc agacccggct ccagctgctt gatctccgcc
     1501 ttggcctcgt ccatggtttt gcctccaagg tcgattccct gaaccacaat gcctttgtaa
     1561 aaggtatcca cgtcgattac ggagctgatc tgcttctggc gctggtattc ctgataaaac
     1621 tgctttccga acacgccgcc caaagcgccg agaataagca gcgccgataa agaaacccaa
     1681 acggctatat gctttttccg tttttggact ttcgccccgg aaaaaatatc cactgttttt
     1741 tcagccgcca ttccggtgcc tcccaagtaa attttagtag cttgttaaat cacattatat
     1801 atctcaaact aataaaagta aagtaacagg aaaattacag cgccccgtcc gggctgcaaa
     1861 ccctgtcccg ttatttacca ttggatatgg caactttact atttatatga tataataatt
     1921 aatataatat aattcaatca aataaaaaag cgtattttag gagtgttaat gtgaaaatat
     1981 taattctttc tgcagctacc ggcggcggac accttcgtgc ttcccacgcc attgaatcat
     2041 acattcggga aaactccacc ggcaatgaag tagcggtagt ggatgcgctg aaaagcatca
     2101 acactgtact ggataaaacc gtatgtgagg gataccattt ccttgcgacc aaaacgccca
     2161 aggtgttcgg ccagctctac cgcaagacca acgaggaaag cattctttcc aaccttgtca
     2221 cccgctttac cagcgtgttc agtcagaagc tgattccgct catggaggag cagaagcccg
     2281 acgtggtcat ctccacccat ccgttcgcaa cggagatggt ttcccattta aaagggaaag
     2341 ggatcgtcac cgcgccgctt atctgcctga tgacggatta cggcccgcac cgcgcgtggt
     2401 tcggcgacca tgtggacgcc tacatcgttt ccaccgcgga tatgattccg gagatggagg
     2461 ccatgggcgt aaagaaagaa aaaatctacc ccttcggcat tccggtgggc gatgtgttct
     2521 ttaccacaaa agagaaacca gagcttcttg aaaaattcgg aatggacgcc ggcaagccga
     2581 ccatcctgat catggcgggg agctttggcg ttaccaacat catgaatatc tataaggata
     2641 tcatccgtgt gcccgccgat ttccagatca ttgtgatcac cgggcgcaac caaaagcttt
     2701 ttgaagcatt tacgccggtc gtggaaaaca gtcccaagaa aaccaagctt gtatttttca
     2761 ccaacgaggt ggaaaattac atgcacgctt ccgacctgat catcacaaag ccgggcgggc
     2821 ttaccgtttc ggaagcgctg gcctgcaata tcccgctggc ggtattcgac gccatccccg
     2881 gccaggagga ggataacgcg aatttccttc tgacgcacga catggccgtc aagctggaaa
     2941 agggcgtgga caccggcaaa accatccaga ctcttctgga agacagccgc cgccttgagg
     3001 aaatgcgcag ctcctgcgaa gagttcgaca cctctgaatc cagcaagaat atcttttcct
     3061 taatcaacga gctgtgtgaa aagaatataa aataataatg aaaacaaaaa ttaagcccgg
     3121 gcagcatttt taacgctgtc cgggcttttg ctttactgtg ttggtattgt tacaggcaag
     3181 attttacgta ctccattggc atcattgtga tttttgaggt ttcttccacg gaaagcccct
     3241 gcttcagaaa tctcttgatt acagcggtca tggtttcacc cacttcaagg atatgtttgt
     3301 ccgggtcata gaagcggatt ccgcgctgtc cccaggggaa tgtctttacc ccgtggacca
     3361 gctcaatctc cgggaatttt ttcagatgct ccaggaagcc gtcaaagtcc tcttcctcaa
     3421 aatacagctc gaagttattg cttttcgtga gaatttcctc ttcggatttg ccgataaact
     3481 ctgcccacgt tttcttcgtc tgcagcgaga aaccgccctc aaacgtaatg ttcgcgccga
     3541 aatcacagat tacctttaac ccgaatacct cttcataaaa ctttctggaa acggaaaagt
     3601 cggccgccga aatcaatggt gcgctgaatt tcatatcctt cactcatttc tataagattt
     3661 gttctctttt tatagtatac ggtcgaactt acattcttca ttgtaaaaag cggacaggtt
     3721 tttcaggtaa tcggtcggag tgacaccgca gatcgattta aa
//
LOCUS       DATALY010000328         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_501748,
            whole genome shotgun sequence.
ACCESSION   DATALY010000328 DATALY010000000
VERSION     DATALY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501748"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VHR42_08865"
     CDS             <1..295
                     /locus_tag="VHR42_08865"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEX3027317.1"
                     /translation="VTIANGGVMPVSADILGTGSTDRALVLLKQGLIPNYGLMGGGTR
                     LKALGDIFRIRVFFLYYLSLGDIMIAVGIFLLIFFLMEPKRIMAGRLRSGKNG"
     gene            490..2274
                     /locus_tag="VHR42_08870"
     CDS             490..2274
                     /locus_tag="VHR42_08870"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX3027318.1"
                     /translation="METIKEYLKNHLLIADGATGTYLSVLAGRSSEPCEMLNLKEPGL
                     VAGAHREYIRSGAGLILTNTFYAGSLALENDPKTAADIIKAGFSIAAEAAGSQAYAAA
                     DFGPVVDGNWEKEQAEEEYRRLADAFLDAGARIFLFETFANDEIPVQTARYIKSRLKD
                     AFVMISFAVLPDGHTRLGVKGDELLRRVIGSGAADSVGFNCCSGPAHLLEYALSQDYG
                     GLYPLIMPNSGYPFRQEDEIVYPGSPQYFGSKLAQAAASGFKIIGGCCGTTPEHIRCL
                     AKSVIQSTAGESNVIRPQPTAGRRESAGSGFRQALQSGRKTAVVELDPPFNSDIARFE
                     AAAGEIAACGVDAITIADSPMARPRADSVAIAARLKRQLGVDVIPHISCRDKNLNAIK
                     SALIAAHIEGIRNVLAVTGDPVPDTDRGSVRSVFNLNSVSLCTFIRDLGSDIFKGDEI
                     FCGCAYNVNARNIDKEIDKLDKKIQAGAGFVLTQPLFTEESVCALERIRKKGVKTIAG
                     ILTPVSYRNAMFLNNEMPGIVLPEETVARFLPEMTREQGEQAGIEISLEIAEKIADLV
                     DGFYFIVPFNRVRVTAKIIRELRDRKIL"
     gene            complement(2362..3156)
                     /locus_tag="VHR42_08875"
     CDS             complement(2362..3156)
                     /locus_tag="VHR42_08875"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX3027319.1"
                     /translation="MIIIGEKINSSIPASKSAIETQDKAQIISLAKTQAEAGADFIDI
                     NAGMFLEEESSLLVFLAKTVTESLGLRLSIDTPNYKAAQAAVEAVGKGGHIINSVTVE
                     KDRLENMTALAREYGCGLVALCMQEADMPENLESRLKIAGKLVETITSRGIGESDIYI
                     DPMLKPLGADENSGLEALETISKLHGLFPGCHISCGLSNLSFGLPKRRLINRAFAVAA
                     ISAGLDAAILDPLDKDLMGLIAAGEAIFGKDEYCMEYIGKCREGLI"
BASE COUNT          779 a          847 c          935 g          848 t
ORIGIN      
        1 ggtgacgatc gcaaatggag gcgtcatgcc ggtctccgct gatattcttg gcacgggcag
       61 cactgaccgc gcgcttgtgc tgctcaaaca gggcctgatt ccgaattacg gactgatggg
      121 cggcggaaca agactgaaag cgcttggcga catctttcgg atccgcgtgt ttttccttta
      181 ttatctgagc ttgggagaca tcatgatcgc ggttggaatt tttcttttga tcttttttct
      241 gatggagccg aaaagaatta tggcgggcag gctccgatcc ggaaaaaacg gctgatttgt
      301 tttttgaaca taaataaacg acacttatca aatgctatta tggcggtgac tgaatgagta
      361 ccgataccgc tgtaatagta atttttgacg ttatcatgat tcactgcgcg ggccgcagtc
      421 gaattgcata ataaaacggt attccggcag aacaagccgg gaaaaatttt gggaacgtga
      481 ggcgccggat tggaaacgat taaagaatat cttaaaaacc acttgttaat tgccgatgga
      541 gcaaccggga cttatctgtc cgttttggcg ggcagaagca gtgaaccatg tgaaatgctc
      601 aatcttaaag aacccggtct tgttgccgga gcccacaggg aatatatccg cagcggggcg
      661 gggctcatcc tgaccaatac cttttatgcc gggtcgctgg cgctggaaaa cgacccaaaa
      721 accgcggcgg acattataaa agcgggtttt tccattgcgg cggaggcggc cggaagccag
      781 gcctacgcgg ccgcggattt cgggcctgtc gtggacggaa actgggagaa agagcaggcg
      841 gaggaggaat accgccgcct tgccgacgcg tttcttgacg ccggcgcgcg gatttttctg
      901 tttgagactt ttgccaacga cgagattccg gttcagacgg cccgctatat caaatccagg
      961 ctcaaagatg cttttgtgat gatttccttt gccgttcttc ccgacggtca tacacggctt
     1021 ggtgtcaagg gtgacgaact gctgcgccgt gtgattggca gcggagcggc ggattcggtc
     1081 ggatttaact gctgttccgg cccggcccat ttgctcgaat acgcgctgtc gcaggactac
     1141 ggcgggcttt atccgctgat tatgccgaat tccggctatc cgttccggca ggaggatgaa
     1201 attgtttatc ccggttcgcc gcagtatttc ggctccaaac tcgcgcaggc tgccgcatcc
     1261 ggttttaaga ttatcggagg atgctgcggc acgacccccg aacatatccg ctgtctggca
     1321 aaatccgtta tccaaagcac cgccggcgag tcgaatgtca tccggcccca gccgacggcc
     1381 ggacgtcggg aaagcgccgg gagcgggttt cgtcaagcgc ttcaatcagg cagaaagacg
     1441 gcggtggtgg agctcgaccc gccgttcaat tccgatattg cccgttttga agcggcggcc
     1501 ggagaaattg ccgcctgcgg agtcgacgcc ataacgattg ccgattcacc gatggcgagg
     1561 ccgagggccg actcggtggc gattgcggcc agactgaagc gtcagctggg cgttgacgtg
     1621 atcccccata tcagctgcag ggataaaaac ctcaacgcca ttaaatccgc cctgatcgcg
     1681 gcgcatattg aaggcatccg taatgttctt gcggtgacgg gcgacccggt gcccgatacc
     1741 gacagagggt cggtcagaag cgtttttaat ctgaattccg tttcactttg cacgtttatc
     1801 cgcgatcttg gcagcgatat ctttaagggt gatgaaattt tctgcggctg cgcttataat
     1861 gttaacgcca gaaacattga taaggaaatt gataagctgg acaaaaaaat tcaggcgggc
     1921 gccggttttg tgctgacaca gcctcttttc accgaagaat cggtctgcgc acttgaacgg
     1981 atccggaaaa agggcgtaaa aacgatcgcc gggattctga ctccggtcag ttaccgcaac
     2041 gcgatgtttc tgaataatga gatgcccggc attgtgctgc ccgaagaaac ggtggcgcgc
     2101 tttttgccgg aaatgacgcg ggaacagggg gaacaggcgg gtattgaaat ttcacttgaa
     2161 attgcggaaa agatcgctga tctggttgac ggattttact ttattgttcc gttcaaccgc
     2221 gttcgcgtca cggccaaaat cattcgggag ctgcgagacc gcaaaatcct gtaagagggg
     2281 gacgtctgcc gcgaagcagc aagacgtccc cctcttcgga atgtttgaat gttcacttta
     2341 actgtattgt ttccggatac gttaaatgag cccctcacgg cattttccga tgtattccat
     2401 gcaatattcg tctttgccga aaatagcctc gcccgccgca atgagaccca tcagatcctt
     2461 atcaagcggg tcgaggatcg ccgcgtcaag accggctgaa attgccgcta ccgcaaacgc
     2521 gcgattaatc agccttcgtt tgggcaggcc gaatgagagg tttgaaagcc cgcaggaaat
     2581 gtggcagccg gggaagagac cgtgcagttt tgaaatggtt tcgagcgctt caagaccgct
     2641 gttttcatcc gccccaagcg gtttaagcat ggggtcgatg taaatatccg actcgccgat
     2701 tccacgggat gtgatcgttt cgaccagttt acctgcgatt ttcagtctgc tttccagatt
     2761 ttccggcata tcggcttcct gcatgcacag ggctaccaga ccgcagccat attcccgggc
     2821 aagcgctgtc atgttttcca gccggtcttt ttccacagta accgagttga tgatgtggcc
     2881 gcctttcccg acggcttcga cagcggcctg cgccgcctta taatttggcg tgtcaatgga
     2941 aagccggagc ccgagggatt ccgtaacggt ttttgccaga aaaacgagca gggaagattc
     3001 ctcttcgagg aacattcccg cgttgatgtc gatgaaatca gctcccgctt cagcctgggt
     3061 tttcgcgagg ctgatgattt gagccttgtc ttgggtttcg atggcgcttt tggacgcagg
     3121 aatggaacta ttgatttttt cgccaataat aatcatattg aaacatcctt tgctgggaat
     3181 ttttaaaaca cggaatttca aggtgggaat cttcttgccg aatccgccca ttctcaaatg
     3241 atacgtcttt cagcatctat tatacaataa aacccgtagt tttttaatcg gtttcaacag
     3301 aaatactggt tttcccgatt attttaatcg aatgttgtgt ccgagaccca atgcacaaac
     3361 cgatcacttt accgccgggg cgcggatttt tctgtttgag acttttgcc
//
LOCUS       DATALY010000329         6129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_156398,
            whole genome shotgun sequence.
ACCESSION   DATALY010000329 DATALY010000000
VERSION     DATALY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156398"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="VHR42_08880"
     CDS             complement(<1..1474)
                     /locus_tag="VHR42_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX3027320.1"
                     /translation="MSKNSIIIKGAREHNLNNVDLEIPREKLIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPDVDYIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEIYDYLRLLYARIGIPHCPVCGREIRQQTVDQIVDKVMSLPAGSKLQILAPVVRAR
                     KGEHIKEFDTARRSGYVRVRVDGNIYDLSEEIKLEKNKKHSIEIVVDRLVLKPEIRGR
                     LADSLETSTALSGGIAIVEVVGGEELLFSQNYACPEHGVSIEELTPRMFSFNNPYGAC
                     KTCGGLGISMKIDPDQIIPDRKLSVKGGAIRASGWSYSDGGTIAQMIYEGLAEHYGFS
                     LDVPVKSLPKEALDVILYGTKGEKIKFTRRNEYGSGSYATDFEGVVNNLERRYKETQS
                     EWMREEIESCMTSVPCPECNGARLKKEILGVTVGEININEFTRMSVTKAVDFLLQLKL
                     SEKDMMIASRIIKEIKERLTFLKNVGLEYLTLSRPAGTLSG"
     gene            complement(1636..3600)
                     /gene="uvrB"
                     /locus_tag="VHR42_08885"
     CDS             complement(1636..3600)
                     /gene="uvrB"
                     /locus_tag="VHR42_08885"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX3027321.1"
                     /translation="MEGFKLVSNYKPTGDQPRAIEKLTAGVNSGMPAQTLLGVTGSGK
                     TFTMANVIANVNKPTLVLAHNKILAAQLCSEFKEFFPENAVEYFVSYYDYYQPEAYVP
                     QTDTYIEKDSAINDEIDKLRHSATSSLSERRDVIIVSSVSCIYSLGDPIDYRNLVISL
                     RPGMTKDRDEVVRKLVEIHYERNDINFVRNKFRVRGDVVEIFPAYSGENAVRVEFFGD
                     EIERITEINTVTGEVKGILNHIAIYPASHYIVPREKMETAVVEIEREMEERVKWFKDN
                     GKLIEAQRIEQRTRYDIEMMREIGFCSGIENYSRVLSGREPGSSPFTLIDYFPDDYLL
                     FVDESHVTMPQVRGMFGGDRARKKNLIDFGFRLPSAYDNRPLNFDEFLDRIHQAVFVS
                     ATPSDFEKDRSSQIVEQVIRPTGLVDPLIIVKPTDGQIDDLLSEINLRSEKGERVLVT
                     TLTKKMAEDLTRYLDGMGVKVRYLHHDIDTIERMEIIRDLRLGEFDVLVGINLLREGL
                     DIPEVSLVAILDADKEGFLRSDTALIQTVGRAARNSGGSVIMYADSVTKSMERAIEET
                     ERRRQIQMDYNTKHGITPKTIIKDVREIIEISSNDDKKDKGSFSKLSHREKETLLAKL
                     EKEMKNAAKLLEFEHAAYLRDKIKELRGSK"
     gene            complement(3633..4373)
                     /locus_tag="VHR42_08890"
     CDS             complement(3633..4373)
                     /locus_tag="VHR42_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX3027322.1"
                     /translation="MKDDNREFDNTHMHKGHRQRIKERFVSEGLDNFEPHVILELILF
                     YALPRKDTNAIAHHLLDYFDGSLSRVFDAPIEELVKIDGIGLNAAILFKMFPEVCRRY
                     LLDLSDTGCIVKNSQDAAKYMIPMFIGKTNEIVTIMCIDSKGKVLFCNGIFEGSVNAA
                     AVSVRRFVEIAVRYATTDVIMAHNHPGGLAIPSEQDIMVTRKVSKALRTVNINLIDHI
                     IISGNDWVSLAATPTTRALFQSNVFLED"
     gene            complement(4386..4967)
                     /gene="pyrE"
                     /locus_tag="VHR42_08895"
     CDS             complement(4386..4967)
                     /gene="pyrE"
                     /locus_tag="VHR42_08895"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX3027323.1"
                     /translation="MSQERVIEILKDAGVLMEGHFLLTSGRHSNKYLQCAKIFKNAKY
                     SEELCSALAEYFKNEKIDLVIGPALGAIQMAYEVSRSIGCENIFAEREEGKMTLRRGF
                     KIEPGQRVLVVEDVVTTGGSVKEVIEIVKKQGGEVAGVGVIVDRSAGAVDFGVPLKSV
                     ISMKVESWLPGECPICKAGEPLVKPGSRKLPTK"
     gene            5172..>6129
                     /locus_tag="VHR42_08900"
     CDS             5172..>6129
                     /locus_tag="VHR42_08900"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3027324.1"
                     /translation="MKQFMIGTNDAGQRLDKFLQKAVKNFPQTLMYKYIRLKRIKVNS
                     KRSEISYRLCEGDSVQLYINDEFFLSPTGNSDFMKAPDKVDVIYEDENILIVDKRPGL
                     VVHEDESGGYDTLIARIQHYLYSKGEYDPDRENSFAPALCNRIDRNTGGMVIAAKNAQ
                     ALRIMNEKIKSREIKKLYLCIIAGKLKEKTGVLEGYLEKDSSENKVYIHKTPQDGDLT
                     IKTGYRVIAEKNGYSLLEVELITGRTHQIRAHFASIGHPLLGDGKYGENSVNRSYGLK
                     WQALYSYKLKFEFTTDAKILQYLDGREFQVPKVWFVGEFFGGL"
BASE COUNT         1540 a         1578 c         1398 g         1613 t
ORIGIN      
        1 cgccggaaag cgttcccgcc ggacgcgaca gggtcagata ttcaaggccc acatttttta
       61 aaaatgtcag ccgttctttg atttccttaa taattcggct ggcaatcatc atatctttct
      121 ctgacagttt gagctgaaga aggaaatcca ccgccttggt caccgacatt cgggtgaatt
      181 cattgatgtt gatttctcca accgtaacgc cgagaatttc ttttttcagc cttgcaccat
      241 tacactcagg gcagggcacg ctggtcatgc aggattcgat ttcttcccgc atccattcgc
      301 tctgagtctc cttgtagcgg cgctcgagat tattgacgac gccttcaaaa tcggtcgcgt
      361 aacttccgct tccgtattcg tttcgccggg taaatttgat tttttcgcct tttgttccgt
      421 acaggatgac atccagcgct tctttgggca gagatttgac cggaacgtcg agtgaaaagc
      481 cgtaatgctc cgccaggcct tcgtaaatca tctgggcgat ggttccgccg tcggaatagc
      541 tccagccgct tgcgcggatc gctccgcctt tgacggaaag cttccggtcc ggaataatct
      601 ggtcgggatc tattttcatc gagattccaa ggccgccgca agtcttgcag gcaccgtaag
      661 gattgttgaa ggaaaacata cgcggtgtca gttcttcgat gctgacgcca tgctccgggc
      721 aggcgtaatt ctgagaaaag agcagttcct ctcccccgac aacttccaca atggcgatgc
      781 ccccggaaag cgcggtggag gtctcaagtg aatcggccag cctgccccgg atttcgggtt
      841 tcagaaccaa ccggtccacg acaatttcaa tgctgtgctt tttgtttttt tccagcttaa
      901 tctcttcgga taaatcgtag atatttccat cgacccgaac ccggacatat cccgaccgcc
      961 gggcagtgtc gaattccttg atatgttcac ccttacgggc ccgtacaacc ggcgcgagaa
     1021 tttggagttt gcttccggcg ggaagcgaca tgactttatc cacaatctgg tcgacggtct
     1081 gctggcggat ttccctaccg caaaccgggc agtgcggaat gccgatccgg gcgtaaagaa
     1141 gccggagata atcataaatt tccgtaacgg ttccgacggt tgaacggggg tttttggacg
     1201 ttgttttctg gtcgatggat atggcgggag aaagcccgtc gatataatca acgtcgggtt
     1261 tttccatctg gccgaggaac tgtctggcgt aggaagacag cgactcgaca tatctccgct
     1321 ggccttcggc ataaatcgtg tcaaaggcga gcgacgattt gccgcttccc gacaggccgg
     1381 taaagacaat cagtttttcg cgcggaatct caagatcgac attattcagg ttatgctccc
     1441 gcgcgccttt gattataatg ctgtttttcg acaagtaata tcatcctttg catgtaatac
     1501 taattccgat gaaacatcgc ctgagagtcg agggaataaa ttccagatga acaacaacgg
     1561 atactttgta gattcagccg taatattttc gcaagatttc atggaaatca gtataaggtc
     1621 ctgccggccc gcgcgttatt ttgagcctcg cagctctttg attttatcgc gcagataagc
     1681 ggcatgctca aactcaagaa gcttggcggc atttttcatc tctttttcca gctttgccaa
     1741 aagcgtctct ttttctctat gggaaagttt cgaaaacgag cctttgtcct ttttatcgtc
     1801 attcgaggaa atctcaatga tttcccgaac gtctttaata atggttttgg gagtgatccc
     1861 gtgtttcgtg ttataatcca tctgaatctg tctgcggcgc tcggtctcct caatagcgcg
     1921 ttccattgat ttggtgacgc tgtcggcata cataatgacg ctgccgcccg aatttctcgc
     1981 cgcgcggcct accgtctgga tgagcgccgt atcactgcga agaaatccct ccttgtcggc
     2041 gtcaagaatt gcgacaaggg aaacctccgg aatatccaat ccctcgcgca gaagattgat
     2101 accgaccaga acgtcgaatt cgccaaggcg cagatcccgg atgatctcca tacgctcaat
     2161 ggtgtcaata tcatgatgaa gataacgaac cttcacaccc atcccgtcaa gatagcgggt
     2221 aaggtcttca gccatttttt tggtcagcgt cgtgacaagt acgcgttcgc ctttttcgga
     2281 acgcaggttg atttcggata aaagatcgtc aatctgccca tcggtaggct tgacgataat
     2341 cagcggatcc acaaggccgg taggccggat cacctgctcg acgatctggc tgctccggtc
     2401 cttttcaaaa tcggacggcg tggcgctgac aaaaaccgcc tgatgaatcc ggtcaagaaa
     2461 ctcgtcaaaa ttaagcgggc ggttgtcata cgcggatggc agccggaacc cgaagtcgat
     2521 cagattcttt tttctggccc ggtctcctcc gaacatcccc ctgacctgcg gcatcgtgac
     2581 atgtgattcg tctacgaaaa ggagataatc atccgggaaa tagtcgatca atgtgaacgg
     2641 cgagcttccc ggttcccggc ccgaaagaac gcgggaataa ttttcgattc cgctgcagaa
     2701 accgatctca cgcatcattt ctatatcgta gcgggtgcgc tgctcgattc tttgggcctc
     2761 gatcaatttt ccgttgtctt taaaccattt gacccgttct tccatttctc tttcaatctc
     2821 aacaacggcc gtctccatct tttcccgcgg cacaatataa tgcgatgcgg gataaatggc
     2881 gatatggttt aaaatcccct ttacctcgcc ggtgacagta ttgatctccg tgatccgctc
     2941 gatttcgtca ccgaagaatt cgacccggac ggcattctca cccgaatagg cggggaaaat
     3001 ctcgacaacg tcccccctga cccggaattt attcctcacg aagttaatat catttcgttc
     3061 ataatggatt tcgaccagtt tacgtaccac ttcgtccctg tctttcgtca tacccggccg
     3121 aagcgaaata accagatttc ggtagtcgat tgggtcgccg aggctgtaga tacaggaaac
     3181 actcgagaca atgatgacat cccgcctttc agacagggag gatgtcgccg aatggcgcag
     3241 cttgtcgatc tcatcattaa ttgccgaatc cttttcaata taagtatctg tctgggggac
     3301 ataggcttcc ggctgataat agtcgtagta acttacgaaa tactccaccg cgttctccgg
     3361 gaagaattcc ttaaattcac tgcagagctg cgccgccaga atcttattat gggcaaggac
     3421 cagcgtaggc ttgttgacat tggcaatgac attggccatg gtaaaggttt tgcccgaacc
     3481 cgttacaccc agcagggtct gcgccggcat tcccgagttc acgcccgcgg taagtttctc
     3541 gatcgcccgg ggctgatcgc ccgtcggttt ataatttgaa accagtttga atccttccat
     3601 aatccctccg gacgggtaaa cccgaacttc ttctaatctt ccaaaaaaac attcgactga
     3661 aacagcgccc gggtcgtcgg tgtggcggcg agcgaaaccc agtcgtttcc ggatatgatg
     3721 atatggtcaa tcagattgat gttgacggtt ctcaacgctt tggaaacctt tctcgtaacc
     3781 ataatatcct gttccgacgg tatcgcaaga ccgccagggt ggttatgcgc catgatgacg
     3841 tcggttgtcg cataacggac ggcaatctca acaaaacgcc gtacgctgac agcagcggcg
     3901 tttacgcttc cctcaaaaat cccgttgcag aacagaacct tgcctttgct gtcgatacac
     3961 atgatggtaa caatttcatt tgtttttccg ataaacatgg gaatcatgta tttcgccgcg
     4021 tcctgcgaat ttttaacgat gcagccggta tcgctcaaat caagcagata ccggcggcag
     4081 acctcgggaa acattttgaa caaaatagcc gcatttaaac cgattccgtc gattttgaca
     4141 agctcctcaa tcggagcgtc aaaaactctc gaaagcgagc cgtcaaaata atccaaaaga
     4201 tggtgggcaa tagcgttcgt atcttttctg ggaagggcat aaaacaggat cagctccaga
     4261 ataacatggg gttcgaaatt gtccagcccc tcagagacaa atcgttcttt aatcctctgc
     4321 ctgtgcccct tatgcatatg ggtattgtca aattctctgt tgtcgtcttt catatcgcct
     4381 catccttatt tagtcggcaa tttccggctg ccaggcttaa caagcggttc tcccgctttg
     4441 caaatggggc attctccggg cagccaggat tcaaccttca tcgaaatgac tgacttcaga
     4501 ggaacaccga agtcgactgc gcccgccgag cggtcaacaa taacgcctac tccggcaact
     4561 tctccaccct gcttcttgac gatttcaata acttccttaa cggaaccccc ggttgtgaca
     4621 acatcctcca ccacgagaac acgctggcca ggctcgatct taaaccctct tctcagcgtc
     4681 attttgccct cttcgcgctc agcgaatata ttttcacagc caatgcttct gcttacctca
     4741 taggccatct gaatcgctcc cagcgcgggc ccgattacca gatcgatttt ttcattttta
     4801 aaatattccg ccagagccga acataactcc tcgctgtatt ttgcgttttt gaaaatttta
     4861 gcgcactgga gatatttatt actgtgtctg ccagatgtca gcaaaaagtg tccttccata
     4921 aggacacccg catcctttaa tatttcaatg actctttcct gactaatcat ttaacatcct
     4981 ccactcgtat ctttatatta atgtttaaaa agcggacaaa aacggatttt cgatattttt
     5041 ttcggaattc aggctgcttc aagactgtat tctattatat aatacatttt gcattttgaa
     5101 aagtgttgaa ggcatgatat aatttgtaaa gaaaacgacc cttccgattt taaaccgata
     5161 ggatgagcga attgaaacaa tttatgattg gaaccaacga cgccgggcag cgtctggaca
     5221 agttcctgca aaaagcagtc aaaaactttc cccagactct aatgtataaa tatatacggc
     5281 tcaagagaat caaggtcaac tcaaagcgca gtgaaatttc ctaccgtctg tgcgagggcg
     5341 attcggtgca gctctatatc aacgatgaat ttttcctaag tccaacgggt aattccgact
     5401 ttatgaaagc ccccgacaaa gtggatgtta tttatgaaga tgaaaatatc ctgattgtcg
     5461 ataaaaggcc ggggcttgtc gttcatgagg atgaaagcgg cggttatgac acgctgatcg
     5521 cgcgtattca gcattatctt tacagcaaag gcgaatatga tcccgaccgt gaaaattctt
     5581 ttgcccctgc cctttgcaac cgaatcgacc gaaacaccgg gggaatggta atcgccgcaa
     5641 aaaatgcgca ggcattaaga atcatgaacg aaaaaatcaa atcccgcgaa atcaaaaagc
     5701 tttacctgtg tatcattgcc ggaaaactga aagaaaaaac cggcgtgctg gaaggctatc
     5761 tggaaaaaga cagctcggaa aacaaggtgt atatccacaa aacgcctcaa gacggcgatc
     5821 tcaccatcaa aaccggttac cgtgttattg cggaaaaaaa cggctacagc cttcttgaag
     5881 tggaactgat caccggccgc actcaccaga tccgagcgca tttcgcaagc attggccatc
     5941 ctcttctggg cgacggaaaa tacggtgaaa actcggtaaa ccgttcctac ggacttaaat
     6001 ggcaggcgct ttattcctac aagctgaaat ttgaatttac aaccgacgcc aaaatcctcc
     6061 aatatcttga cggccgggaa tttcaggttc ccaaagtctg gtttgtaggt gaatttttcg
     6121 gcggcctga
//
LOCUS       DATALY010000330         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_156560,
            whole genome shotgun sequence.
ACCESSION   DATALY010000330 DATALY010000000
VERSION     DATALY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156560"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VHR42_08905"
     CDS             complement(<1..611)
                     /locus_tag="VHR42_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX3027325.1"
                     /translation="MLQKLMDKSFKALEYDRILEMLSRECTSPDSAKMAMSLRPCPDI
                     GEAQTELGQTEDACRLSLRFGGPSFFGLMNIASALNRAEIGSSLMMKELLDVARVLSV
                     IRSLARYREMSASESTGLDELFDSLSPNKYLEDKITSSIVSEEEVADDASHELSDIRR
                     HMKRCGVRVREQLDKLVRSSSYQKYLQESIVTIRGGRFVVPVKS"
     gene            complement(687..1436)
                     /gene="recO"
                     /locus_tag="VHR42_08910"
     CDS             complement(687..1436)
                     /gene="recO"
                     /locus_tag="VHR42_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX3027326.1"
                     /translation="MLIKTDGLTIRETNVGENDRILTILTREKGVVSASARGSRKLKS
                     RIAPASQILCHADFTLFKSKEKYIINEAEPLNFFMGVRKDIERLTLAQYFAQVCGYAV
                     PEEEPAGTALRLALNSLHYLENGKRPQKLIKAVFEMRMLTDTGYMPDLVACAECGEFE
                     DDVMYLRPQSGNLICSRCAGAAKEKYPLSKGALAAMRHTIYAEFERLFSFSLTGPALE
                     QFSAAAEGYLLYQMGRSFPTLEFYHSLSAIG"
     gene            complement(1381..1569)
                     /locus_tag="VHR42_08915"
     CDS             complement(1381..1569)
                     /locus_tag="VHR42_08915"
                     /inference="COORDINATES: protein motif:HMM:NF025372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HEX3027327.1"
                     /translation="MGTNFVFSKDSVWTLFEKTGRIDAYMLYYDLTSSQTMTSQEASR
                     DADKNRRSDHSGDKRRGK"
     gene            complement(1733..>2439)
                     /gene="era"
                     /locus_tag="VHR42_08920"
     CDS             complement(1733..>2439)
                     /gene="era"
                     /locus_tag="VHR42_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX3027328.1"
                     /translation="FHRPRNRLGEYMVKTVSDSILDVDAAIFVVEPVEGANDYEKALL
                     DKFRSAAIPVILVINKIDTLAQKQQLAGVIQTWSERFDFHAIVPASAARCEGIADVVK
                     EAVGLMPEGPQYYPEDMFTTEPERAVVAEIIREKLLRLMSEEIPHGTAVSIERMKERE
                     EGGVIDIDAVIFCEKESHKGMIIGKGGQMLKKVGSQARMDIERLLDCHVNLKLWVKVK
                     EDWRNRESILKSLGYE"
BASE COUNT          570 a          712 c          561 g          596 t
ORIGIN      
        1 gatttgaccg gaaccacaaa ccgcccgccc cggatggtca caatggattc ctgaagatat
       61 ttctggtagg acgatgatcg aaccagcttg tccagctgtt cgcgaacccg aaccccgcac
      121 cgcttcatat gccggcggat gtcggaaagc tcatggctcg cgtcatcggc cacttcctcc
      181 tccgaaacga tggatgaggt aattttatct tcaagatatt tattgggcga gagggaatca
      241 aacaattcgt ccagaccggt ggattcgctc gccgacatct cccggtagcg ggcaagcgag
      301 cggatcaccg acagcacccg cgccacatca agcaattctt tcatcatcag ggagctgccg
      361 atttccgccc tgttcagcgc cgacgcgata ttcatcagcc cgaaaaacga agggcccccg
      421 aatctcaggg aaaggcgaca ggcatcctcc gtctgcccaa gctccgtctg cgcctcgccg
      481 atatccgggc agggccgcag agacatcgcc atctttgcgc tgtcagggct ggtacattcc
      541 ctcgacagca tctcgagaat ccggtcatat tcgagggctt taaacgattt atccataagc
      601 ttttgaagca actgatcact tccccgtata aaaagagttc ctgcggccgt ccccggcagg
      661 atccagtaaa atgtaaatct ctccgcctaa ccgatcgcac tcagactatg ataaaattcc
      721 aatgtcggga acgaccgtcc catctgataa agcagatatc cttctgcggc tgcggaaaac
      781 tgctccagcg ccggcccggt cagtgaaaag gaaaaaagcc gctcaaattc ggcataaata
      841 gtatgcctca tcgcggccag agctcctttg gaaagaggat atttctcttt tgccgccccg
      901 gcgcaccggg agcaaatcag attgcccgac tggggcctga gatacatgac gtcatcttca
      961 aattcgccgc attccgcgca tgcgacaaga tccggcatat aaccggtatc ggtcagcatc
     1021 cgcatttcga aaaccgcctt gatgagcttt tggggccttt ttccattttc cagataatgc
     1081 agtgaattaa gcgccagccg cagcgcggtt ccggcgggtt cctcctccgg aacagcataa
     1141 ccgcagacct gggcaaaata ctgagcaagc gtaagtcgtt cgatatcttt gcgaacgccc
     1201 ataaaaaaat tcaggggctc cgcttcgtta attatgtact tttccttgct cttaaaaaga
     1261 gtgaagtcgg catggcacaa aatttgggaa gccggagcaa tccggctttt taattttcgt
     1321 gatcctctcg cggacgcgct gacaacaccc ttttcccggg tcaatatagt cagtatgcgg
     1381 tcattttccc cgacgtttgt ctcccgaatg gtcagaccgt cggtttttat cagcatccct
     1441 acttgcctcc tgactggtca tggtctggct gctggtcaaa tcataataaa gcatatacgc
     1501 atcaattctg ccggtttttt caaatagagt ccacaccgaa tccttgctga aaacgaaatt
     1561 agtccccatc gttgtctcct cccggccctg tgcgctgaat caagtccaca gcgcaccatt
     1621 ttaccaatgc atcggaatac atcggtacga ttatttttta cctcgcgtac cggctctatt
     1681 cgagggaaat attgttttaa caaagctcca tgcggtctta gccccgcaga gtttattcgt
     1741 aacctaaaga tttgagaatg ctctcgcggt ttctccagtc ttccttgact ttaacccaga
     1801 gtttcaggtt aacgtggcag tcaagcagcc tttcgatatc catacgggcc tgacttccga
     1861 cctttttgag catctggccg ccttttccga taatcattcc cttgtggctt tctttttcac
     1921 agaatatgac ggcgtcaatg tcgatcacgc cgccttcctc ccgttccttc atccgctcga
     1981 tggaaaccgc cgtgccgtgg gggatttcct ccgacatcag cctgagcagc ttctcccgga
     2041 tgatctcagc cacaaccgcc ctttccggct cggtcgtgaa catatcctcc ggataatact
     2101 gcgggccctc gggcatcagc ccgaccgctt ctttcacaac atcggcaatg ccctcgcatc
     2161 tggcggcgct ggcgggaaca atcgcgtgaa aatcaaaccg ctccgaccag gtctggatga
     2221 ctccggcgag ctgctgtttc tgagccagcg tatcaatctt gttaatcaca agaataaccg
     2281 ggattgcggc cgacctgaat ttgtcaagca gcgccttctc ataatcattg gcgccttcga
     2341 ccggctccac aacaaaaatg gcggcgtcaa cgtctaaaat cgaatcactg accgttttaa
     2401 ccatatattc gcccagtctg tttctgggac ggtgaaaac
//
LOCUS       DATALY010000331        11208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_330633,
            whole genome shotgun sequence.
ACCESSION   DATALY010000331 DATALY010000000
VERSION     DATALY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330633"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..828)
                     /locus_tag="VHR42_08925"
     CDS             complement(223..828)
                     /locus_tag="VHR42_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX3027329.1"
                     /translation="MWSYDKRLEYPIKIKQTNPQLAKIIISQYGGPDGELGASLRYLS
                     QRYSMPYDEVKGTLTDIGTEELAHFEMVGAIVHQLTKNLTEEEIKKGGFADYFVDHTI
                     GVYPLAASGTPFTASYVAVKGDTITDLTEDMAAEQKARTVYDNILRFADDPDVRDPIK
                     FLRQREIVHFQRFGESLRVVQDHLNAKNFYAFNPSFDNGKR"
     gene            complement(831..1097)
                     /locus_tag="VHR42_08930"
     CDS             complement(831..1097)
                     /locus_tag="VHR42_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HEX3027330.1"
                     /translation="MNEKGMLLNNLSAVAFSMVDLHLFLNTHPENASAIALFSQYRQK
                     YMALVAEYERMYGPLSAVNGVQDNRWAWIRSPWPWEYGANTEVR"
     gene            complement(1094..1336)
                     /locus_tag="VHR42_08935"
     CDS             complement(1094..1336)
                     /locus_tag="VHR42_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HEX3027331.1"
                     /translation="MDQDIMKDLYDPKDREISAFPSQTPVGMAYVPVQKFRSVYEPDV
                     GLMQGTIFPDLDKPWLGNRAFSDHIENAGMERGMGQ"
     gene            1687..2607
                     /locus_tag="VHR42_08940"
     CDS             1687..2607
                     /locus_tag="VHR42_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3027332.1"
                     /translation="MSQKTKLKLFFGTILTVIILYYSVKSLGELNPQELFRQKINWGL
                     AILSVLVFMYSNYIRGLAYTRGIDPGLDRMTAFRIVGIGHAANMVLPLRAGEGLRLAF
                     FPERYGAVERTKLLIIPGFADFVAIMLLALPSVPFAGFKDPVLLKVLWILVFLILAVG
                     ALGAVIVYLVPKLRGYAKQYLNPASLKMMYWVVLSWIIMLVSTWIGLLSLGYPALESA
                     RMSLAVFTATNIVSFIPASPGGIGLFEYGVILGLGGLGVKPAPALSIGLMIHLMQYAA
                     LVPLGAVLYVTAIGGKFRKSFGARPRPKQK"
     gene            complement(2704..3684)
                     /locus_tag="VHR42_08945"
     CDS             complement(2704..3684)
                     /locus_tag="VHR42_08945"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEX3027333.1"
                     /translation="MYYLDNSGTDPYWNLALEEFLLKNSKAEYFMLWQNRPSIIIGRN
                     QNTLSEINEDYVKEHSIPVVRRLTGGGAVFHDLGNLNYTFLVNDSGGVGFDFKKHTAP
                     IVEVLRGLSVNAELSGRNDLTIDGKKFSGNSQYRYKGRLLHHGTLLFSAVLSDVSAAL
                     KVDPSKFEGKGVKSIRSRVTNISEHLTQKLSLPEFKSLISDHIKKSSRDFQIYHLKEQ
                     EIAATERLAKEKYSTLGWNYGVSPDCNFSKRKKFSAGSVEVYMTVKDGVIRSLRIFGD
                     FFGECEIGDIESALENVNYREEEIRQRLAAFDLSRYFSGIDLEDLLGVLF"
     gene            3899..4297
                     /locus_tag="VHR42_08950"
     CDS             3899..4297
                     /locus_tag="VHR42_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3027334.1"
                     /translation="MLNVDKFSSVPIYEQITQQFLKLIISGSLKPEDQIPSVRSLSME
                     LSINPNTIQKAYNELESRGITSSVPGVGRFVSKNAPAILREYYSEKLDEMRDTVYTLA
                     EANVDEERVLALIRSVYENVKNDKEKRSKS"
     gene            4294..5193
                     /locus_tag="VHR42_08955"
     CDS             4294..5193
                     /locus_tag="VHR42_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027335.1"
                     /translation="MIRVDSLTKKFDGFTALNDISCTIPDGCVYGLVGSNGAGKSTFI
                     RLLAGIYKPDKGKVMINGASVFENPAVKNKLVLVPDDLYFLSQSNMNKMAELYAAVYK
                     NFSYPRFEKLTDAFGLDPKNNISNFSKGMRRQAVTILALSSMPEYLFFDETFDGLDPV
                     MRNLVKSVIYSDVCGRKTTVIITSHSLRELEDTCDQLAMLHEGGVVFESDIQNLKTSL
                     FKIQIAFDEDFNRDRFTGLDLLSFSKTGRVASLIARGNKEQTLADLKSMNPVLLDVLP
                     LSLEEVFVYEMDALGYSVREMIN"
     gene            5208..7352
                     /locus_tag="VHR42_08960"
     CDS             5208..7352
                     /locus_tag="VHR42_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027336.1"
                     /translation="MEKKYFDKKLYLNGLKQLRLTGSIILILTVFSTALPALLMILDA
                     KRQRTGYTPITSMIVIQLSVMSLIAPIILCLTLFSFLNSRKGSDFYHSLPNTRVSIFW
                     SFAASVLSWLLLSLMFSLIITVVIYASLGASIIWSLLPFLILTQLSGMALITAAMLLA
                     MSITGTYLTNLTVFALILLLPRCITTMFTYTLKQKLRVITFHEFGIFGDPNYNIPIKF
                     ILYPFNTGLQIDSSQNGLITSSGSILYTFILALVYFGLACLLFHLRKSETAGRSAPNR
                     ILQHVFRCAVTLPVALIIPLSIVLTENSFLYWSERRTLLIAVITISLIVYFLFELITT
                     KKLKNLPRSAPVLLFVVAIDFIFVFFAYFSMNRIYNTKPVASDMESVAIQLENADNTS
                     NRTQYFSDLSLKALSLRDSKALSAVSASLAKTIDKSKSQSNNLSLNSFVKIKLKSGRT
                     LERYVDMTKNQYQIISEAESKDASYQKALASLPTHGNIQSLAVTDLSASESKRLWDTF
                     QSEYKSLSFSDKEAAVMYDKQQSNGYYIPYSGYSASSFASIHIMGFVGLENFESYYPI
                     GIKTPKTLRLYVELENQKYKDILVKLLQKSMKEKNSTYSYTITAMNFSVDSSSSKTLL
                     NYGNQSTVIAQNSVYDSDDDGSPNRSSDHPIKNDKTIFALLQKQINDPIDLKQPIIEI
                     SLFSDNTNGCYSFYMPVSEDSAVQIVNLKQNS"
     gene            7516..8190
                     /gene="deoC"
                     /locus_tag="VHR42_08965"
     CDS             7516..8190
                     /gene="deoC"
                     /locus_tag="VHR42_08965"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX3027337.1"
                     /translation="MPVTEKQLAAMTDGVITSVLITRQELLDFLDFTKKCHLRSVIGP
                     RCYTALIREKLNASGTLLSSECSDSNGADATHIKIYTAQHALQLGADEIEMRMNFGYL
                     RSGMYQEVVQDIRAVRNAVGGHILKCAIEAPLLSYDEIIKACDLAVEGGADFVSSSTG
                     ESGETTAHQIKIISDAVGGRAKIKAAGGIRTLAQIDEFASLGVECFGIAHNMIANLIS
                     AASNRS"
     gene            8206..8883
                     /gene="deoC"
                     /locus_tag="VHR42_08970"
     CDS             8206..8883
                     /gene="deoC"
                     /locus_tag="VHR42_08970"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX3027338.1"
                     /translation="MMKLNGQQFARMLDGTLTATNTTKAQYQAHLETAIKYHFYSVIG
                     LRCYIPMTVEALKETDVLPGSGCCLASGADATEVKVYAGKSNVALGAKELDMVMNLSY
                     FKSGLYDLVVDDIRRVKEAVGNIPLKCIIEAPLLTDEEIRTACALVVEGGADYLKTAT
                     GQAGPTTLHHIEVISGVLKGRIKIKAAGGIRKIETVEQMTDLGVSRFGVGSASAIKLI
                     QAFNDRS"
     gene            8901..10430
                     /locus_tag="VHR42_08975"
     CDS             8901..10430
                     /locus_tag="VHR42_08975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX3027339.1"
                     /translation="MASYLLSHDIGTSASKATLFHYNGEISKSSTIAYEAEYSNGTWA
                     EQDPRQWWDAFCQNNQTILEGIDPAEVAAVSISGQMMGCLPVDQDCKPLYSSIIWADG
                     RSVQQAEELLKTIGNEEFFPITGMTISPNYGIEKAMWVKKHMPGIFGKTYKFLQSKDY
                     ITCRLCGEFVTEASDAQYMHCYDIKTGDWSGRVLRAAGIDRDKLPGLVKSGTLLGKVM
                     PSIAQECGLSPCTAVVEGIGDGRASLLGTGVIELGDAYISLGTSSWVGMIVKAREPVY
                     QPASHQAVVPFLNPDYIVDGGTMQAGGLSLNWLKKELCRYEQSLSEKGGAGVYQLIDS
                     EVLKSSAGSNGVIFLPYLMGERDPWWNTEAKAAFLGLKAESSRADLYRSVLEGVAMNL
                     AAILRMSGNLEEIKSMRITGGGAKSRVWRQIFADVLDMPIIKTNIGDEAGSLGTAVLA
                     GIGIGLYRDYSVIQSFHKIEEITYPIPSNVSVYQKMQPVFEDSYRALTGINHRLSVIF
                     S"
     gene            complement(10619..>11208)
                     /locus_tag="VHR42_08980"
     CDS             complement(10619..>11208)
                     /locus_tag="VHR42_08980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3027340.1"
                     /translation="TAAYCHCDVCSPADIKQLVKYTAETFGDIDILVNNAGGGGLPLH
                     FEEISDGLWEKIVHLNLTASFHTIREVFPYMKAKRSGKIVNISSGYGVTGGEYCAHYA
                     SAKAGLIGLTMSVAREVGEYHINCNCIATPTTDTPGLRESDFEFVPEEIPFIPMGRIA
                     MPEDIANCVLYLVSDAADYMTGQIVAPNGGRRMPI"
BASE COUNT         2956 a         2723 c         2532 g         2997 t
ORIGIN      
        1 cctgtaattt tttgcttttc caattatagc ataatttgtt gataattttt acattttatt
       61 gatgaaactt cccacaaaaa agacaaatta tcttcttctt tttgtctact tgtgtcatat
      121 tcattgcggg tttggcagac aataaaaagc ggagggatta ataagcagcg cataagaaac
      181 agccttccga tttggaaggc tgtttcttat ttgtgtataa ttttatcttt ttccattgtc
      241 aaaggacggg ttaaacgcgt agaaattctt cgcattcaaa tgatcctgta cgacacggag
      301 agattcaccg aaccgctgga aatgtacaat ttcgcgctgc cgaaggaact taatcggatc
      361 ccggacatcc ggatcgtcgg caaaccgcag gatattgtca taaacagttc tcgctttttg
      421 ctctgccgcc atatcctctg tcagatcggt aatcgtgtct cctttgactg caacatagga
      481 agcggtaaac ggcgttccgg aggctgcgag gggatatacg ccaatggtat ggtcgacaaa
      541 atagtcggcg aatccgccct tcttgatttc ctcttccgta agatttttcg tcagttgatg
      601 tacaattgct ccaaccattt caaaatgcgc gagttcttcg gtgccgatat cggtcagcgt
      661 cccttttact tcatcataag gcatggagta acgctggctc agataccgca acgaagctcc
      721 cagctcaccg tcaggtccgc catactgcga aatgataatt tttgcaagct gaggattggt
      781 ttgcttgatt ttaatcggat attccaaacg tttgtcataa ctccacatga ctatctcacc
      841 tccgtatttg cgccgtattc ccacggccat ggcgaacgaa tccatgccca tctgttgtcc
      901 tgaaccccat tcacagcaga aagcgggccg tacatccttt catattccgc aacgagcgcc
      961 atatattttt gccggtactg gctaaacagg gcaatcgcgc ttgcgttttc gggatgtgta
     1021 tttaaaaaca gatgaaggtc gaccatactg aacgcgactg cgctcaggtt attcagaagc
     1081 ataccttttt cattcattgc cccatccccc tttccatgcc ggcattttcg atatgatcgg
     1141 aaaatgcccg gtttccaagc cacggcttgt caagatccgg gaatatcgta ccctgcatca
     1201 aaccgacatc aggctcataa accgagcgga atttctgaac cggaacatag gccattccaa
     1261 ccggggtctg agacggaaag gcactgatct cccggtcttt tggatcgtaa agatccttca
     1321 tgatatcctg atccatgatt gtgtctcctt ttttagtttg agattcaatc cagtttatgt
     1381 ccacatttgc atcgcggtga ataccgccgg ccgacggaaa aatcatgaac atgctgaaac
     1441 ccgtccaagt ctttaaaatt tgtcccactt tctcatattt tccgtcggat ggttccgaaa
     1501 gattattttt ctacggggct ttattccgtt attccgcata caccggtttt ttctccgcat
     1561 attaaattgt aactgccagt catttctgat ttcatcgcaa ggtcagattt ttttcggttt
     1621 tttcttccgg aaagaaagca tggggcggcc catgggccgt aattttattg gatcgggagc
     1681 agtttcatgt cgcagaaaac aaaactcaaa ctgtttttcg gcaccattct cactgttatc
     1741 attctgtatt attcggtcaa atcgcttggt gaactgaatc cgcaagagct ctttcgtcaa
     1801 aagatcaact gggggctcgc gattctgtcc gtgctggtct ttatgtactc aaactatatc
     1861 cgcggccttg cctatacgcg cggaattgac cccggacttg accggatgac tgcattccgg
     1921 attgtgggaa tcggacacgc ggccaatatg gtcctccccc tgcgtgccgg cgagggatta
     1981 agacttgcct tttttccgga acgatacggc gcggtggagc gtacaaagct tctgatcatt
     2041 ccggggtttg cagattttgt cgccattatg ctgctggcgc ttccatcggt tcccttcgcg
     2101 ggctttaaag atcctgtatt gctgaaagtt ttatggattc ttgtattcct gattcttgcc
     2161 gttggagcat tgggcgcggt gatcgtatat ctcgtgccca aactgcgcgg ctacgcgaaa
     2221 caatatctga atcccgcctc actcaaaatg atgtattggg tcgttctgtc atggattatt
     2281 atgctcgttt ccacatggat cggcctctta tccctgggct acccggcgct tgagtcggca
     2341 cgcatgtctc tcgcggtatt caccgcaacc aatattgtca gctttatccc cgcttcaccc
     2401 ggaggaattg gattatttga atatggcgta attcttggcc ttggagggct tggcgtaaaa
     2461 ccggctcctg cgctttcgat cgggctgatg atccatttaa tgcagtatgc cgcactggta
     2521 cctctgggcg ctgttctata cgttactgcg atcggaggaa aattcagaaa atcattcggc
     2581 gccagacccc gcccgaaaca aaaatagccg gcttagaaaa gacaacgttc tgagaaacag
     2641 ggcggccgaa caattgttcg gccgccctgt ggatcaattt cagaaaacga atggcttttc
     2701 acattaaaac aggacgccca gcagatcttc aagatcaata ccggaaaaat agcgggaaag
     2761 atcaaaagcg gcaagccgct gtctgatttc ctcctcgcga tagttcacgt tttcaagcgc
     2821 tgattcaata tcgcctatct cgcattcacc aaaaaaatcc ccaaaaatgc gcagacttcg
     2881 gattacaccg tctttcaccg tcatatatac ttcaacgctc ccggcggaaa atttctttcg
     2941 ctttgaaaag ttacaatcgg gagatacgcc gtaattccag cccaaggtcg agtatttctc
     3001 ttttgcaagc cgttctgtgg cagcgatttc ctgttccttc agatgataaa tctgaaaatc
     3061 ccgactggat tttttaatat ggtctgaaat caggctttta aattccggca gagacagttt
     3121 ttgggtcaga tgctccgaga tatttgttac cctgctccga attgatttca cacctttgcc
     3181 ttcaaatttt gacggatcaa cctttaacgc cgcagaaaca tcggacaaaa ccgcgctgaa
     3241 aagcagcgtg ccgtgatgca agagacggcc cttataacga tattgagagt ttccggaaaa
     3301 ctttttccca tctattgtca ggtcattgcg gcccgaaagt tcagcgttga cagaaagtcc
     3361 cctgagaact tcgacgatcg gtgcggtatg ttttttaaag tcaaatccca ctcccccgct
     3421 atcgttgacc agaaacgtat agttgagatt tccaagatca tggaacacag cgcctccgcc
     3481 ggtcagcctg cgaaccacag gaatcgaatg ttcctttaca taatcctcgt tgatttccga
     3541 cagcgtattc tgatttcttc caatgatgat actgggccgg ttttgccata acatgaaata
     3601 ttccgccttg gagtttttaa gcaaaaattc ttcaagcgca agattccagt atgggtccgt
     3661 tcccgaatta tccaaataat acacaaccga cactctcctt ctacccggca gaatattacg
     3721 cagcgctttt taagtgtgcg cttccatctt acctcctata acatatcaca tccgagactc
     3781 atgtcaagtc agggcggatg aagattcaaa ccaactcttg acattttaaa ttttatttta
     3841 taaaataagt gtactaacat tactaataca cctatgacga aagggaggaa ttttattttt
     3901 gttaaatgtt gataagtttt caagcgtacc gatttatgag cagattaccc aacaattttt
     3961 aaaactaatc atcagcggca gtttaaaacc ggaagaccag attccctccg ttcgttccct
     4021 ttccatggag ttatccatca atccaaatac aatacaaaag gcctacaacg agctggaatc
     4081 gcgtggtatc acttcatccg tacccggggt cgggcggttc gtctcaaaaa acgcgccggc
     4141 catactcaga gaatattaca gtgaaaagct ggatgaaatg agggacaccg tttacactct
     4201 tgcagaagcg aatgtggacg aggaaagagt tctggcgtta atcagaagtg tttatgaaaa
     4261 cgtaaaaaac gataaagaaa agaggagtaa gagttgatca gggtcgatag tttaacaaaa
     4321 aaatttgacg gtttcactgc gctgaatgac atcagctgta cgatacctga tggatgcgta
     4381 tacggcttgg taggctcaaa cggagccggg aaatccacct ttatccggct gcttgcggga
     4441 atttacaaac cggacaaagg aaaagttatg atcaatggcg cttcggtttt tgaaaatccg
     4501 gcggttaaaa acaaacttgt tctggtgccg gatgaccttt attttctttc acagtcaaat
     4561 atgaataaaa tggctgaact ctacgcagct gtttataaaa atttcagtta cccgcgtttt
     4621 gaaaaattaa ccgacgcatt tggtcttgac ccgaaaaaca acatcagtaa cttttcaaaa
     4681 ggaatgcgcc gtcaggctgt cacgatactc gcgctttcct ccatgcctga gtacctgttt
     4741 tttgatgaga cctttgacgg gcttgacccg gtcatgcgaa atcttgttaa aagcgtcatc
     4801 tattcggatg tttgcggacg gaaaacgacg gtcattatca cttctcacag tctgagagaa
     4861 ttagaagata cctgcgacca gctcgcaatg ctccacgaag gcggagttgt ttttgaaagc
     4921 gatattcaga atttaaaaac atcccttttt aaaattcaaa tcgcgtttga tgaagatttc
     4981 aaccgggatc ggtttaccgg tcttgatctt ctgagtttct caaaaaccgg cagagtcgca
     5041 tcgcttattg cacggggaaa taaggagcag acccttgccg atctgaaatc aatgaatccg
     5101 gttcttcttg atgtgctccc cctttccctt gaagaagtgt tcgtgtatga aatggacgcg
     5161 cttggatatt ctgtcaggga aatgatcaat taaagggggg aattcggatg gaaaaaaagt
     5221 attttgacaa aaagctttac ttaaatggtc tcaagcagct tcggctaacc ggaagtatca
     5281 ttctgatctt aacggttttc agcaccgctt tgcccgcgct gctcatgatt cttgacgcaa
     5341 aacggcagag aaccggctat acgccaatca catcaatgat cgtaatacag cttagcgtga
     5401 tgtctttaat tgccccgatc attctgtgcc tgacactgtt ttccttttta aacagccgga
     5461 aaggctccga tttttatcac tctctgccca atacccgcgt cagcatattt tggagctttg
     5521 cggcctccgt gctgtcatgg ctgctcctat cgctcatgtt ttctttgatt atcaccgttg
     5581 tcatttacgc ctcattagga gcgtcgatca tctggagctt gctgcccttt ttaattctga
     5641 cccagctaag cggaatggcg ctgattaccg cggcgatgct gctcgccatg agcatcaccg
     5701 gcacctatct gaccaatcta accgtattcg ccttgatttt attacttccg cgttgtatca
     5761 caactatgtt tacttatacc ttaaagcaaa aattgcgagt cattaccttc catgaattcg
     5821 gaatattcgg cgatccgaat tacaatatac ccataaaatt tattctttac ccattcaata
     5881 ccggactgca aatcgattcc tctcaaaatg gtctgattac ctcatcgggt tccattctat
     5941 atacattcat tctcgcgctg gtttatttcg ggttagcctg cctgcttttt catcttcgca
     6001 aatcagaaac tgccggccgg agcgcgccca accgcattct gcagcatgtt ttccgctgcg
     6061 cagtcacgct tcccgtcgct ctgatcatcc cgctttcaat cgtgttaaca gaaaattctt
     6121 ttttgtactg gagcgaaaga cgtacccttc ttatcgcagt tattacaatt tccctgatcg
     6181 tttatttcct gtttgaatta atcactacaa aaaagctgaa aaatttaccc agatcagcgc
     6241 ctgttttact atttgttgtt gcaatcgatt ttatttttgt atttttcgct tatttttcga
     6301 tgaaccgcat ttacaacacc aaacctgtcg catccgatat ggaatctgtc gcaatccagc
     6361 ttgaaaatgc cgacaataca tcaaatcgaa ctcaatattt cagcgatctc tccctgaaag
     6421 cgttgtcact gcgcgattca aaagcgctgt ctgcggtatc cgcttctctt gcaaaaacaa
     6481 ttgacaaaag caaaagccag agcaacaact taagtttaaa ttcatttgta aaaataaaat
     6541 taaaatccgg cagaactctg gaacgatatg ttgatatgac caaaaatcaa taccagatca
     6601 tttctgaagc tgagagtaaa gatgcttcct atcaaaaggc cctggcctcc cttccaaccc
     6661 atggaaacat tcaaagcctt gccgttactg acctatccgc cagcgaatcg aaaaggctct
     6721 gggatacctt ccagtcggaa tataaaagcc tgagtttttc cgataaagag gctgccgtca
     6781 tgtatgacaa gcagcagtca aatggttatt acattcccta ttccggttat tcagccagtt
     6841 cctttgcttc gatccatatt atgggctttg tgggtttgga aaacttcgag agttattacc
     6901 caatcggtat caagacccca aagacgctcc gtctttatgt cgagcttgaa aatcagaaat
     6961 acaaagacat tcttgtcaaa ttacttcaaa agtccatgaa ggaaaagaat tcgacataca
     7021 gttacaccat tacggcgatg aattttagcg ttgattcaag cagttccaaa acattgctca
     7081 attatggaaa tcaatcaact gtaatcgctc aaaacagcgt ttacgacagt gatgatgacg
     7141 ggagtccaaa ccggagttcc gatcatccga tcaaaaacga taagacaata ttcgctctgt
     7201 tgcagaaaca gattaacgat ccgatcgacc tgaaacagcc gatcattgaa atttcactct
     7261 tttccgacaa taccaatggc tgctattctt tttatatgcc tgtcagcgaa gattccgctg
     7321 ttcaaatcgt caatctgaag cagaattctt gacgcatagc agccgccctg atcatcaggc
     7381 gcaggggtag attccaatcg caattctaaa caccggacac gtttcatata tttgaagcgt
     7441 gtccatttct gtgttattgg caggattcat tttattcggt tatcaacaat acgataggga
     7501 aagaaggtcc gtaatatgcc ggttactgaa aagcagcttg ccgcaatgac cgatggggtc
     7561 atcacttccg tactcattac ccgtcaggag cttcttgatt ttctggattt cacgaaaaaa
     7621 tgtcatctga ggtccgtaat cggtcccagg tgctatactg cgctgattcg tgagaaatta
     7681 aacgcaagcg gaaccctgct ttcatcggag tgctccgatt cgaacggagc cgacgcaacg
     7741 cacatcaaaa tttatacggc ccagcacgca ctccagcttg gagccgatga aattgagatg
     7801 cgcatgaatt tcggctatct gcgatcaggc atgtatcagg aagtcgttca ggatatccgg
     7861 gcagttcgaa acgccgtcgg cggccatatt ctcaaatgtg cgattgaagc cccgcttctg
     7921 agctatgatg agattattaa ggcatgcgat ctggcggttg aaggcggagc ggatttcgtt
     7981 tcctcctcaa ccggagaatc cggagaaacg acggcgcatc agatcaaaat tatctccgac
     8041 gcggttggcg gccgggcaaa aataaaggcg gccggcggca tccgaacctt agcccagatt
     8101 gacgagttcg cctcactcgg cgtcgaatgc ttcgggattg cgcacaacat gatcgccaat
     8161 ctgatttcag ccgcatcgaa tcggtcatag agaaggaagg aattaatgat gaaacttaac
     8221 ggccaacagt ttgccagaat gctggacgga acactgacgg cgaccaacac cacaaaggcg
     8281 caatatcaag cccatttgga aacagcaatt aagtatcatt tttattctgt catcgggctg
     8341 cgctgctaca tacccatgac ggtcgaagcc ttaaaagaaa ccgacgtact gcccggaagc
     8401 ggctgctgtc ttgcttccgg agcagatgca accgaagtca aggtttatgc gggcaaatcc
     8461 aatgttgctc ttggagcaaa agagctggat atggtaatga atctctccta ttttaaatcc
     8521 ggtctttatg atctcgttgt cgacgatatc cgaagggtga aagaggctgt tggaaacatt
     8581 ccgcttaaat gcatcattga agcgccgctt ttgaccgacg aagaaatccg aacggcttgc
     8641 gctctggtag tcgagggcgg ggccgattat ttaaaaactg caaccgggca ggccgggccg
     8701 acaacgctgc atcatattga agtcatttcc ggcgtgctga aaggacggat taaaatcaag
     8761 gctgccggcg gtattcgaaa aatcgagacc gttgaacaga tgacggatct gggcgtttcc
     8821 cgctttgggg tcggctcagc cagcgccata aaactgattc aggctttcaa cgaccgctct
     8881 taagacagga ggtccacggg atggccagct atctgctctc acatgatatc ggcacatcag
     8941 cgagcaaagc gacattgttt cattataacg gtgaaatatc aaaaagcagc accatcgcct
     9001 atgaagcgga gtactcgaac ggaacctggg cggaacagga cccccgtcag tggtgggacg
     9061 ccttctgtca gaacaatcaa accatcttag aagggattga cccggctgaa gtagccgctg
     9121 tatccatcag cggtcaaatg atggggtgtc tcccggtgga tcaggactgc aagccgctgt
     9181 actccagcat catctgggcg gacggaagat cggtgcagca ggccgaagaa cttctgaaaa
     9241 caatcggaaa cgaggagttt ttccctataa ccggtatgac gatcagcccc aattacggca
     9301 tcgaaaaggc tatgtgggtt aaaaaacaca tgcccggaat tttcggtaaa acttataaat
     9361 tccttcagtc caaggattac atcacctgca gactctgcgg tgaatttgtt accgaggcaa
     9421 gcgacgccca atatatgcat tgttatgata tcaaaacagg cgactggtcc ggccgcgtac
     9481 tcagggccgc cggaatcgac cgtgacaaat tacccggact tgtaaaaagc ggcacgctgc
     9541 tgggcaaagt aatgccgtcg attgcgcagg aatgcgggtt atctccgtgc acagccgttg
     9601 ttgaaggaat cggcgacgga agagcttcgc tgctgggaac cggcgtcatt gagcttgggg
     9661 acgcttacat cagtctggga acatccagct gggtcggaat gattgttaag gcccgggagc
     9721 ccgtttatca gcctgcctcc catcaagcgg tcgtgccgtt tctgaacccg gactatattg
     9781 tcgacggcgg aaccatgcag gccggtgggc tcagcctcaa ctggctaaaa aaagaactct
     9841 gccgctatga gcagtcgttg tcggaaaaag gcggcgccgg tgtttatcaa ttgatcgaca
     9901 gcgaggtgtt aaagtcttcc gccggttcga acggcgtaat atttcttccc tatctgatgg
     9961 gggagcgcga cccctggtgg aatacggaag cgaaagccgc ctttcttgga ctaaaagcgg
    10021 aaagttcgcg agccgactta taccgaagcg tacttgaggg cgtagccatg aatctggcgg
    10081 ccattcttcg gatgtcgggc aatctcgagg aaatcaaaag catgcgaatt acgggcggcg
    10141 gagcgaaaag ccgtgtctgg cggcagattt ttgccgacgt ccttgacatg ccaatcatca
    10201 aaaccaacat cggggacgaa gcgggcagcc tcggcaccgc tgttctggcc ggtattggaa
    10261 tcggccttta ccgcgattac tcggtgattc aatcctttca caaaatcgaa gaaatcacgt
    10321 acccgatccc ctcaaacgtt tccgtctatc aaaaaatgca gcccgttttt gaagactctt
    10381 atcgggcgct gacgggcatc aatcacaggc tgagcgtaat tttttcataa accggcaaca
    10441 agattgaaga agaaaaatga gattcaaaag gttttgtatg caaacagtca cctgtttgag
    10501 cgaatgcaaa acggcggcaa actgccgccg ttttgttttc attttaaggg cgtattttat
    10561 tgatcatacg gaaaggagag aggaaatttt ctgaaatgat aaatcggatc gttatccgtc
    10621 agataggcat ccgcctgccg ccgttcggcg ccacaatctg ccccgtcata taatcggccg
    10681 cgtcggacac cagataaagt acgcagttgg caatatcctc cggcatggcg attctgccca
    10741 tcggaataaa aggaatctcc tccgggacaa attcaaaatc agattcacgg agtccgggcg
    10801 tatcggtcgt aggcgtcgca atacagttgc agttgatatg atactccccg acttccctcg
    10861 ccaccgacat ggtcagcccg atcagccctg ctttggccga agcatagtga gcgcagtatt
    10921 ccccgcccgt gacaccgtag ccggaactga tgttgacaat ttttcccgat cttttcgcct
    10981 tcatgtaggg aaaaacttcc cggatggtat ggaacgaagc cgtcaaattg agatgaacga
    11041 ttttttccca taacccgtcg ctgatttctt caaagtgaag cggcaggccg ccgccgcccg
    11101 cgttgttcac cagaatatca atatccccga aggtctccgc cgtgtatttg acgagctgtt
    11161 taatgtcggc cgggctgcag acgtcgcaat ggcaataggc tgccgttc
//
LOCUS       DATALY010000332         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_502952,
            whole genome shotgun sequence.
ACCESSION   DATALY010000332 DATALY010000000
VERSION     DATALY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502952"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VHR42_08985"
     CDS             <1..750
                     /locus_tag="VHR42_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3027341.1"
                     /translation="ILATIMYANNEIGTIQPIPELARICRDKGVIFHTDAVQAIGHVP
                     INVREQNIDLLSLSAHKFHGPKGVGALYIRKGLRFSNLIEGGAQEKGRRAGTENVPGI
                     VGLGKAIEIACTDIETRNEKVRKLRERLLDGVLKLEAVRLNGDRQRRLAGNANFSVEG
                     IEGESLLLMLDMNGICASSGSACTSGSLDPSHVLLSIGLRHEVAHGSLRISLDESNTQ
                     DDIDYILEVLPQIIGRLRAMSPVWEEMQKTK"
     gene            797..1234
                     /gene="nifU"
                     /locus_tag="VHR42_08990"
     CDS             797..1234
                     /gene="nifU"
                     /locus_tag="VHR42_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HEX3027342.1"
                     /translation="MAYSQKVMDHFMNPRNTGEITDANAVGEVGNPVCGDIMKMYLKI
                     NNNVIEDVKFKTFGCGAAVATSSIATEMIKGKTIEEALKLTNKAVIEALDGLPPAKIH
                     CSVLAEQAVKSAISDYYKSQGIDPKPIVGEIMECEACHVHDPE"
     gene            complement(1395..>1696)
                     /locus_tag="VHR42_08995"
     CDS             complement(1395..>1696)
                     /locus_tag="VHR42_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027343.1"
                     /translation="FMQWCGQSLHLLILQQPYNVLQRSHLGWVKRGQKQSRHSSRNLR
                     QISPSQQILGPMVNGDSHLQQLRPLILSLIIKPSAERSFQTFAASGFFQLYHPGG"
BASE COUNT          456 a          403 c          438 g          399 t
ORIGIN      
        1 attctggcaa cgataatgta cgcaaacaac gagattggta caattcagcc tattccggag
       61 cttgcccgaa tatgccggga taaaggcgtt attttccata ctgatgcggt acaggctatc
      121 ggccatgttc cgatcaatgt ccgggagcag aatatcgacc tgctgtcact ttccgctcat
      181 aaatttcatg gaccaaaggg cgtcggcgcg ctgtatatcc gcaagggtct gcgcttttcc
      241 aacctgattg agggcggagc gcaggaaaaa ggcagacgtg cggggactga aaatgttccg
      301 ggaattgtcg gacttggcaa ggccatcgag attgcctgca cagatattga gacaagaaac
      361 gaaaaagtcc ggaagctcag agaacggctg ctggatgggg tcttgaaact cgaggcagtc
      421 cggctcaacg gcgaccggca gcggcgtctg gccggaaacg ccaatttctc ggtcgaaggc
      481 attgaaggag aatctcttct tctgatgctg gatatgaacg gcatctgcgc ttcttccggc
      541 tccgcctgca catccggctc gctggatccc tcccatgttc ttctttccat cgggctccgg
      601 catgaggttg cgcacggatc actgcggatt tcactcgatg aaagcaacac ccaagatgat
      661 attgattata ttctcgaagt tcttccccag attatcggcc ggctgagagc catgtccccg
      721 gtttgggaag aaatgcagaa gacaaaataa ttgaaacata cggctgcctg tcgggcggcg
      781 gaaagaaggt ttttttatgg catacagtca gaaagtaatg gatcatttta tgaatccacg
      841 taataccggt gaaatcacag atgccaacgc ggtaggggaa gtcggaaatc ccgtctgcgg
      901 cgatatcatg aaaatgtatc tgaagatcaa caataatgta attgaagacg ttaaattcaa
      961 aaccttcggc tgcggagccg cggtggcaac ctcctcgatc gccactgaaa tgatcaaagg
     1021 taagacgatt gaagaggcgc ttaagctgac caacaaagct gtcattgagg cgcttgacgg
     1081 tctgccccct gcaaaaatac attgctccgt tttggccgag caagcagtaa aatcagccat
     1141 ctcggattat tataaaagtc agggcatcga ccccaagccc attgtgggtg aaattatgga
     1201 gtgcgaagcc tgtcacgttc acgacccgga ataagagaac aaagcttaaa gccctgcgct
     1261 gtatgcgcag ggcttttttg tcaaacctgt cctgttatga atagaatgga gtagaccctt
     1321 tctcctttta cacttttcat atcagttatt acaacgccgg ttgacaatcg gcagctgtat
     1381 cacaatctcc caaatcagcc gccgggatga tacagttgga aaaagccgct ggcggcaaac
     1441 gtttgaaacg aacgttcggc agaaggtttg ataatgagag aaagaatcaa cggtcttaac
     1501 tgctgcagat gcgaatcacc gtttaccatt gggccgagaa tctgctgtga cggagatatc
     1561 tgccgcagat tccgcgaaga atgccgcgat tgtttttggc ctctttttac ccatccgagg
     1621 tggcttcgct gcagaacatt atatggctgc tgcaatatca gaagatgcag ggactgaccg
     1681 caccattgca taaata
//
LOCUS       DATALY010000333         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_503149,
            whole genome shotgun sequence.
ACCESSION   DATALY010000333 DATALY010000000
VERSION     DATALY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503149"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VHR42_09000"
     CDS             <1..886
                     /locus_tag="VHR42_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEX3027344.1"
                     /translation="KGTFDSYLWQIQEQKLRYITQVMSGKSIARSCEDMDETVLTAAE
                     VKAIATDNPMLAEKMEVDNEVARLKLLRGSWQNEHAVLERNINQYYPESIAHHRSNIK
                     KISADVELLRKTEGRDFQITIDGRTFNERVPAGERLMLLSRLDDSKEKDTPLSVGEYR
                     GLALALEHGAFDSLQFTLKGSHTYRGDLGSSELGAITRIENAADQIAKLLSNEKDELA
                     NFERQLTEAKKEVVMPFEYEQRLSEYAARQSEINTSLEFQELQKQEDVILDENSETEE
                     GENANIKEESVCVGAEVE"
     gene            complement(1013..1438)
                     /locus_tag="VHR42_09005"
     CDS             complement(1013..1438)
                     /locus_tag="VHR42_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3027345.1"
                     /translation="MDDELKIKMSAFTLDCKDPHELAKFYAELLKWEIPFYDEEYTIV
                     GAPGTNQGAYPGITFQRNPEYKPPVWPEEPEAQQQMAHIDFAVNDLEKAVQYAIQCGA
                     TIADKQFSDSWTVMFDPAGHPFCLCQMKQLFESPSFALL"
     gene            complement(1539..2069)
                     /locus_tag="VHR42_09010"
     CDS             complement(1539..2069)
                     /locus_tag="VHR42_09010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX3027346.1"
                     /translation="MKKALIVVDYQKDFVIGSLGFPKAEALDETIAAKIQEYRSSGDD
                     IIVTLDTHTEDYLETQEGKSLSVIHCVRGTAGWDLFGETAKQIRETDRKFEKGTFGSL
                     ALAEHLKRQGYEQVELVGVVSNICVISNAVLAKAALPESLIIVDAACTASNDDIMNEK
                     ALDVMEGIQIKVINRR"
     gene            2261..2428
                     /locus_tag="VHR42_09015"
     CDS             2261..2428
                     /locus_tag="VHR42_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027347.1"
                     /translation="MDIDQKIQELQEKKKQLQGEKEQQEVQQLLEAARATGLTPAELV
                     KQLAEQKKSEK"
BASE COUNT          723 a          564 c          589 g          688 t
ORIGIN      
        1 aaaagggact tttgattcgt acctgtggca gattcaggag cagaaactgc ggtatatcac
       61 gcaggtcatg agcggaaaaa gcatcgcccg ttcctgtgaa gacatggatg aaaccgtact
      121 gaccgccgcc gaggtgaagg cgattgccac cgataatccc atgttggcgg agaaaatgga
      181 ggtggataac gaagtagcac gcttgaagct cctgcgtgga agctggcaga atgaacacgc
      241 cgttctggaa cgcaacatca atcagtatta tccggaaagc atcgcacatc accggagcaa
      301 tatcaaaaaa atcagcgcgg atgtggaact attgcggaaa acggaaggcc gggattttca
      361 gattacgatt gacggcagga cgtttaatga acgtgttccc gcaggggaac gcctgatgct
      421 gttatcccgt ttggacgatt cgaaagaaaa agacacgccg ctctctgtag gggaataccg
      481 tggcctggcc cttgctttgg agcacggagc cttcgattcg ctccagttca cactgaaagg
      541 ttcacacacc tatcgcggtg atcttggttc atcagaattg ggagcaatta cccgtattga
      601 aaatgctgcc gatcaaattg caaagctcct ttccaatgaa aaagatgagc tcgccaattt
      661 tgagcggcag ctcacggaag cgaaaaaaga agtcgtcatg ccgtttgagt acgagcagcg
      721 tttatcggaa tacgcggcgc ggcagagcga aatcaacact tccctcgaat ttcaggaact
      781 gcaaaagcag gaggatgtga ttctggatga gaatagcgaa acggaggaag gtgaaaacgc
      841 aaatataaag gaagaatctg tgtgtgtcgg tgccgaggtc gagtaagaat atttattgaa
      901 agtatactat ttacaaaaag caggccggtt tctaaagtaa ggaatcggct tgcttccaaa
      961 aactgaaatt tatgaacttc ttataatgct tatactatca gctcttacct ttctatagca
     1021 atgcaaaact gggactctca aaaagttgtt tcatttgaca taagcaaaaa gggtgtccgg
     1081 cggggtcaaa cataactgtc caactatcag aaaattgttt atctgcgatt gttgctccgc
     1141 attggattgc gtattgaacc gctttttcta aatcattaac tgcaaagtcg atatgtgcca
     1201 tttgctgctg agcttcaggc tcttccggcc acacaggcgg tttatattca gggttccgct
     1261 gaaatgttat accgggatac gctccctggt ttgtccccgg agcacccacg attgtatatt
     1321 cttcatcata gaacggtatt tcccatttga gcagttccgc atagaatttc gctaattcat
     1381 gcgggtcttt gcagtccagt gtaaatgcac tcattttgat tttcagttca tcatccataa
     1441 tgttagacct cctataatat aaataattgg tttcctcatg atcgttttaa tgtcgtacct
     1501 gttaaataat gagtaaaaaa gacctaatca cataatgttt accgacggtt gatgaccttg
     1561 atctgaatac cttccatcac atctagggct ttttcattca ttatatcgtc gttgctcgcc
     1621 gtacaagcgg catctactat aatcagcgat tcaggaaggg cggcctttgc cagaacagcg
     1681 ttggaaatca cacagatgtt tgacactacg ccgactagtt caacctgctc atagccctgc
     1741 cttttcaaat gttcagccaa tgccagcgag ccgaaggtcc ctttttcgaa tttccgatcc
     1801 gtttcccgaa tctgcttcgc tgtttcccca aacaggtccc agcctgctgt tccacggacg
     1861 caatgaatta cagatagact tttaccttcc tgtgtttcca gataatcttc tgtatgggtg
     1921 tccagcgtaa cgatgatgtc atcgccggaa gaacggtatt cctgaatttt ggcggcaatc
     1981 gtttcgtcaa gtgcttccgc ttttggaaag ccgaggcttc cgataacaaa atctttctga
     2041 tagtccacaa caattaaagc ttttttcata agctgccgcc tttccggttg cgtttttaat
     2101 cgataaattt atcatatcat actttcttgt tgtattctac ctattcatcc ggtttgagac
     2161 ttgcttttaa agggaaaaat aagtatagta gaagcatatt aatacggaag gaccgatgtg
     2221 tatgccacgc ggagttcgga agtcagtcga agagcaaatt ttggatattg atcaaaaaat
     2281 tcaggaactt caggaaaaga aaaagcaact tcagggggaa aaagaacagc aggaagtcca
     2341 gcagcttttg gaagcagcaa gagcgacggg ccttactccc gccgagctcg taaaacagct
     2401 tgcggagcag aagaaatccg aaaaatagaa ttccttataa tattcatcta tcatggcgct
     2461 gtgtaaaaac ggcgccttat ttttttgctc tgtcattcga tatgacgaag cttcttcatt
     2521 cattggaggt gaggttcatg ccgatggcgg ccacaaaagc cccg
//
LOCUS       DATALY010000334        18642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_846284,
            whole genome shotgun sequence.
ACCESSION   DATALY010000334 DATALY010000000
VERSION     DATALY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18642
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846284"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..474
                     /gene="rsfS"
                     /locus_tag="VHR42_09020"
     CDS             85..474
                     /gene="rsfS"
                     /locus_tag="VHR42_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX3027348.1"
                     /translation="MKREAVFLEPRELLCETAKILDNKKALDIRAIDIEGVSILADYF
                     LLASGTSSTQVKALAEEVEFRMSGLGVEPLRVEGEQTASWIIMDYGSVVIHIFHTQTR
                     EFFNLERLWADGKFLSLEEMTQTPTDK"
     gene            523..2928
                     /gene="leuS"
                     /locus_tag="VHR42_09025"
     CDS             523..2928
                     /gene="leuS"
                     /locus_tag="VHR42_09025"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX3027349.1"
                     /translation="MRYDFAAIEKKWQDIWENKGVFHAKNDYTLPKFYALVEFPYPSG
                     QGLHVGHPRSYTALDIIARKKRLQGYNVLYPMGWDAFGLPTENFAIKTHIHPEIVTKN
                     NIARFKQQLRSLGLSFDWSREINTTDPAYYKWTQWIFLKLFEKGLAYKKEMAVNWCTS
                     CKVVLANEEAVGGVCERCGGEVIRKVKSQWMLKITEYAQRLIDDLDEVDYIERVKTQQ
                     KNWIGRSTGAEIDFSTTCGDTLTVYTTRPDTLFGATYMVISPEHPAIEKWASRISNLD
                     EIRDYQEAASHKSDFERTEVQKDKTGVRLGGVAAVNPANGKEIPIFISDYVLVTYGTG
                     AIMAVPGHDTRDWEFARKFDLPIIEVVKGGNVEKEAFTNCETGVLVNSGFLDGKTVDE
                     AKAVMVDWLTSKKIGRQKVNYKLRDWVFSRQRYWGEPIPLVYCEKCGWVPVPEDQLPL
                     RLPEVASYEPTENGESPLAKMEDWVNTTCPRCGGKAKRETDTMPQWAGSSWYFLRYCD
                     PHNDQELASPEALKYWLPVDWYNGGMEHTTLHLLYSRFWHKALYDLGIVPTSEPYRKR
                     TSHGMILGENGEKMSKSRGNVVNPDEIVNECGADTMRLYEMFIGDFEKAAPWSSASIK
                     GCRRFLERVWALQDVVTSQEDYSRELESSFHKTVKKVTGDIDHLKFNTAIAAMMSLIN
                     EIYDKGSVTAGEFKTLLVLLNPFAPHITEELWETMNYGGMITTRPWPEYDESKCKDES
                     VEIAVQICGKLKGKVTIPAQASESEAFRAATDLQKIDALIQGKNIVKTIYVQGKLINI
                     VAK"
     gene            3094..3267
                     /gene="rpsU"
                     /locus_tag="VHR42_09030"
     CDS             3094..3267
                     /gene="rpsU"
                     /locus_tag="VHR42_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX3027350.1"
                     /translation="MAEIRIKDNESLDSALRRFKRQCARAGVLAEVRKREHYEKPSVK
                     RKKKSEAARKRKH"
     gene            3433..3939
                     /locus_tag="VHR42_09035"
     CDS             3433..3939
                     /locus_tag="VHR42_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEX3027351.1"
                     /translation="MWLVPDKIFEKISDIDPAFIDSQGVKGLVLDIDNTMAPKHIPLP
                     DETLKNWIRNIQSAGISLFVISNNRMNRVSRFAKALELPYIYSGMKPFPHSFRKAVRE
                     MNLAPEEVAAVGDQIYTDVMGARLTGIKAWLVSPVEPNESFFFRLRRRFEAPVVRRYY
                     TGREEKKK"
     gene            3936..4805
                     /locus_tag="VHR42_09040"
     CDS             3936..4805
                     /locus_tag="VHR42_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3027352.1"
                     /translation="MNPFFGPAGSDDRFAAQGYRSVMQAPEYLSRMGLDAYEYQGGRG
                     IRISEENAVQFGKKAREFSIRLSVHAPYYISLSGVDEMKRAGSVDYILQTARAAKWMG
                     AGRIIVHSGSCAKISRLQALEYAKDTLRKALSALDEQGLSDIHICPETMGKINQLGTV
                     EEVMELCSIDERLIPCIDFGHVNARTNGGLKSKRDYLKVLEAVENKLGGERLRHFHSH
                     FSKIEYTEKGGEKRHLTFDDREYGPEFEPLAELCCQKGCSPVFICESSGTQAEDALRM
                     KKIYQDVAEGNEF"
     gene            4805..5242
                     /gene="aroQ"
                     /locus_tag="VHR42_09045"
     CDS             4805..5242
                     /gene="aroQ"
                     /locus_tag="VHR42_09045"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX3027353.1"
                     /translation="MSKHKILVIHGPNLNLLGERETGIYGKDSTEHINREIVDSADEL
                     NVECEIHQFNVEGDIINMLHTARTRFDGIIINAGAYTHYSIAIRDAIAAIKIPCIEVH
                     MSNVYAREEFRHHSVLAPVCVGQISGFGKNSYLLALRGLIDII"
     gene            5347..6414
                     /locus_tag="VHR42_09050"
     CDS             5347..6414
                     /locus_tag="VHR42_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3027354.1"
                     /translation="MKYIEMLQQSLPDGMDGVIIFSEHNRRYFTGFDSSAGLLLVTRD
                     AACFLTDFRYIEAAKTQAAGCEVRLLEDQPKQLPMLFDEFKVKTCGFETRDLPFADYI
                     NLKKDLPGVEFNQTTIADDIIERLRMVKSDEEIALIKKSQEITDAAFRHVIEMIKPGV
                     AERDIALEIEFFMKKNGASGPSFDLIVVSGEKSSLPHGVPGERKFQNGDFITMDIGAL
                     YHGYCSDMTRTVALGSLSDEQKKVYNLVLKAQLEAEKAVRPGIACNEVDRIARDMIYS
                     AGYTGCFGHGLGHSTGLQIHEQPRFSPTCRTILRKGMIMSVEPGIYLAGRFGVRIEDL
                     VLVSDTGCEILTQSPKELMIL"
     gene            6659..7216
                     /gene="efp"
                     /locus_tag="VHR42_09055"
     CDS             6659..7216
                     /gene="efp"
                     /locus_tag="VHR42_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX3027355.1"
                     /translation="MLSAGDFRNGLTFEMDGNVFSVVEFQHVKPGKGAAFVRTKLKNV
                     ITGAVVEKTFNPSDKYPSAFVERKDMQYLYSDGELYYLMDKETYEQMPVNAETLGDNF
                     KFVKENMDVKVLSYKGNVFGIEPPFFVELQVVETDPGFKGDTATNATKLAKLETGASI
                     KVPLFVNEGDMIRLDTRTGEYMERA"
     gene            7361..7786
                     /locus_tag="VHR42_09060"
     CDS             7361..7786
                     /locus_tag="VHR42_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027356.1"
                     /translation="MTVTEKVAYLKGLVEGLGVDEGTKEGRIIKAIVDVLDDMALSVT
                     DMEDNLSEISEQVDAVDEDLEDLEKDFYGDEDEDDEDEDDTDYYEVTCPNCGEKVYLD
                     DELLSDGKMNCPNCGEKLEFDFGCDCCDDCSGHDDEEDE"
     gene            8186..8383
                     /locus_tag="VHR42_09065"
     CDS             8186..8383
                     /locus_tag="VHR42_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027357.1"
                     /translation="MLFGDKPRYFPIDISDETDLNDLDAEQELLPFHPMYSGTKVSNK
                     KTVRSRKKLKNKESERKQEEN"
     gene            8505..8957
                     /locus_tag="VHR42_09070"
     CDS             8505..8957
                     /locus_tag="VHR42_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEX3027358.1"
                     /translation="MKFSDLITEMILRMLSEADGIVEIQRNEMANRLGCVPSQINYVI
                     TSRFTPEQGYFVESRRGGGGYIRITRIKYDRDQMLMHIVNSIGDRIDAESARIFIDNL
                     SDSGIVGEESARLMRASLSNTVYRVVPQEIKDNLRAEIFKRMLLSLLK"
     gene            8971..9495
                     /locus_tag="VHR42_09075"
     CDS             8971..9495
                     /locus_tag="VHR42_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX3027359.1"
                     /translation="MLCDRCGKNEATTYLKEVINGNVKELHLCSRCAEQLGYDNVFGS
                     FNPFDMLGVNVGNFLGGFFSQSLPEQTSESSKKCSFCGTTFEDLAKTAKAGCANCYSE
                     FYQEILPSLQRIHGKTRHVGKIPGRAGKELKLRRELNTLKQKLNDAVSAQEYEQAAQL
                     RDEIKELEKKVQGE"
     gene            9492..10496
                     /locus_tag="VHR42_09080"
     CDS             9492..10496
                     /locus_tag="VHR42_09080"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEX3027360.1"
                     /translation="MKWYLEVGPEGDVVISTRIRLARNVAEYPFPCKMTVEQGKKVAQ
                     LARASLNGSENYEFIDMQKLTPARAMSLAERHLISPEFAQNRDGRALLLSEDESVSIM
                     LNEEDHIRIQTMFSGMQLEKALEAAGNIDDIMDAKLNYAFNENLGYLTQCPTNLGTGL
                     RASLMLHLPAIAQSGAIPNLANAVSKLGFTVRGTYGEGSEAKGAFFQISNQITLGLSE
                     EEAIRNLKDVAGQIISQERAARENLKKLGPQLEDRVWRSYGALSQARMLSADEFMSLI
                     SDVRLGVTAGLIQNLKLEEIHLLIYEVQPASLVAAAGRNLDAQGRDVLRAGIVREKIR
                     "
     gene            10688..13150
                     /locus_tag="VHR42_09085"
     CDS             10688..13150
                     /locus_tag="VHR42_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX3027361.1"
                     /translation="MYEKFTEKAAESLGLAMQAAREMGHSYIGSEHLLLGLLREGSGV
                     AAKALEANHISSDEVEKFISELIGRGEPSNVSPADLTPRGKRIIELAYLESRNLGNNY
                     VGTEHLLMGLLREGDSVAVRILAELGCDPRALYEEIVRSIGSEPAEPAGKSANLGQVK
                     YTGGQKQAGNTPTLNQFGGDLTAQARDGRLDPVVGRSDEIQRVLQILSRRTKNNPCLI
                     GEPGVGKTAIAEGLAEKIVEGRVPELLKDKRVVTLDMSGMVAGTKYRGEFEERIKKAL
                     EEVKKAGNVILFIDELHTIVGAGAAEGAVDAANILKPLLARGEIQVIGATTINEYRKY
                     IEKDAALERRFQPVTVGEPSQEESIAILKGLRDKYEAHHKVKITDSALEAAVKLSARY
                     ITDRFLPDKAIDLMDEAASRLRLKAFTAPPDLRELDDKLKKLSEEKEAAVKSQDFERA
                     AKLRDEEKSLKETVQSQKTEWKHKNERSVNEVTDNEIAEIVSSWTGVPVKKLAQEESD
                     RLLKMEEILHQRVIGQEEAVSAVSRAIRRGRVGLKDPKRPVGSFIFLGPTGVGKTELC
                     KALAESLFGDESSMIRIDMSEYMEKHTISRLIGAPPGYVGYDEAGQLTEKVRRKPYSV
                     ILFDEIEKAHPDVFNILLQILEDGILTDSQGRKVDFKNTVIIMTSNVGARLITEKHKL
                     GFGMSDDSLTQSDKAIKSDVMGELKRTFRPEFLNRVDDIIVFHQLSEENIKKIAGRML
                     NILTKRVGTYDISIDFSDEVVSKVSKEGFDSVYGARPLRRAIQNEIEDRLSEEMLQGR
                     IKAGDQVNTVLKEDKITFEKVK"
     gene            13335..13409
                     /locus_tag="VHR42_09090"
     tRNA            13335..13409
                     /locus_tag="VHR42_09090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13367..13369,aa:Gly,seq:ccc)
     gene            13616..13969
                     /locus_tag="VHR42_09095"
     CDS             13616..13969
                     /locus_tag="VHR42_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027362.1"
                     /translation="MVLECWVIILILGIAAYMFVRGGRKIWASGVLPLMLVPFADIIY
                     SPIGRRLARHSVAYSNGIRLSIYGVLFIIVSIWVILWAKRLPSGKSKYTYIIISIGFT
                     LTLILLFIKNLHVLG"
     gene            14125..14200
                     /locus_tag="VHR42_09100"
     tRNA            14125..14200
                     /locus_tag="VHR42_09100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14158..14160,aa:Gly,seq:gcc)
     gene            complement(14358..15161)
                     /locus_tag="VHR42_09105"
     CDS             complement(14358..15161)
                     /locus_tag="VHR42_09105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3027363.1"
                     /translation="MNFDLVLVTLTYRNDKDLTDFLDSLASLGCSYRVIVVNSFYDNE
                     SESAIRSVAQAYGCDFFSVENKGYGSGNNAGIRFALEHYQFQYLAVSNPDIRIGEFDL
                     DRLTAAENAVVGPKILTLRGKNQNPIHYRRNFFVLFFRFLYAKAGFLPFFLAAILFNK
                     LEKAVLRKGDAKGPRQVYALHGSFFLLPRSVLQTFGGKLFDERMFLFCEENHIAELAR
                     KHGVPIIYDPRFWVTHKEDGSISLENLDHFRITRESLKVFFENWKLFHR"
     gene            complement(15203..16231)
                     /locus_tag="VHR42_09110"
     CDS             complement(15203..16231)
                     /locus_tag="VHR42_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3027364.1"
                     /translation="MANSCKISVAVGTFNGEAYIEEQLRSLFSQTRLPDEIIISDDHS
                     SDRTLALADEVLASFKSHGNIRVKILKSDRNLGVVGNFARAFEACTGDILFPCDQDDV
                     WKPDKIKIMADIFESAPGCSVVFSNAELADSELRPLGRSLWSSLNFFPFMVGRGKEYE
                     NIFRLLLNRCVVTGTAMAFRRSLLPDAMPLSPFWIHDGWLAILCSCLSEVRPVDQCLL
                     SYRQHEKNVVGANSSDPFSRVRRYLGNFSAVRKIRLQRRNRYQDVLNRLEDLGRLPAP
                     EDLSELKRCIAFWNRKLSLDSRGFFQGFAIIVSDCLNGGYRKFYSGFRGAVRDFFCLF
                     HRGNRPAR"
     gene            complement(16271..17554)
                     /locus_tag="VHR42_09115"
     CDS             complement(16271..17554)
                     /locus_tag="VHR42_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX3027365.1"
                     /translation="MKLMNLIKENRLFKNMGYLTALQMTNYLFPLVTIPYLYRVIGIG
                     GYGVLSLAQSVISYFVLLVNFGFYLSGPRLVAIHRENKEKISEIFSTILIIKSILMLV
                     SFFTITLLAASIPIMRVNFMAYFFTMLSLVGEVLFPAWFFQGMESMSYITALNVVSKL
                     IALVLLLITVRGPGDLNLACAIQALSSVLAGIMAVFPIFFKYGVKLRMPERGVLKSYM
                     TENWNLFLAQFSTSIFNNANPVLLGFFVSNTAVGYYAAADKAVRLVVSLISPVTTAVY
                     PRATRLFAKSTEQALAFVKRVLTYGVFFFLAVSAALFASAGLITFLMTGRNVPEITCI
                     MRILSIIPTTIFINNIYGTQIMVNCGMEIKFRNIILFCAVFLLGSSIILIKFYSAVGA
                     GIASLLAELLLAALMVLNIEGNKKLSLFKNGFKLT"
     gene            complement(17618..18379)
                     /locus_tag="VHR42_09120"
     CDS             complement(17618..18379)
                     /locus_tag="VHR42_09120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3027366.1"
                     /translation="MALDNRLVSIIMPVYNKEKYIAQSIESVLSQSYGEWELLIVDDC
                     SSDRSEEIIENYSRFDGRIHYERLDRNGGASRARNAALVKARGRYVAFLDADDVWRPM
                     KLQKQLHLLEASRAGFCFTAIEMIDEDGHQVKSKRKIRPVVDYRYLLTNTVIPCSSVL
                     IDRSATGDFRMPEIPKGQDYATWLSILKKGQLAFGIDEALVLYRLVKDSVSANKLSAL
                     RRTWRIYRKSEHLGLLKSIYCFSLYTLHAFKKYFF"
     gene            18438..>18642
                     /locus_tag="VHR42_09125"
     CDS             18438..>18642
                     /locus_tag="VHR42_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3027367.1"
                     /translation="MNYSIIKLFFLLNCKKSALKFTIWLQSSQTSEVSFPIHLNSATK
                     EDLTALPGIGDTLAQRIIDYRSQH"
BASE COUNT         5188 a         4127 c         4852 g         4475 t
ORIGIN      
        1 attaaaaagc agagcggaaa ggcgacttca agcgcctctt cctcatccga cagttcatcc
       61 aaaacatcat cctgattgca ggaaatgaaa cgggaggcgg tttttctgga accaagagag
      121 cttttgtgcg aaacggcaaa aattcttgat aataaaaaag cgctcgatat tcgggccatc
      181 gacattgaag gggtcagtat tctggccgat tattttcttc tggcaagcgg aaccagctca
      241 acccaggtca aggctcttgc cgaagaagtg gaatttcgta tgtccgggct tggcgttgag
      301 ccgctgcgcg tggagggcga acagacggcg tcctggatta tcatggatta cgggtcggtc
      361 gtcatccata tctttcatac tcaaacccgt gagtttttta atcttgagcg gctttgggcc
      421 gacgggaaat ttttgagcct ggaagaaatg actcaaactc cgacagacaa ataggttatc
      481 agccgcgccg ttattgcgct taaaaataca ggaggggtaa atttgcgtta cgactttgcc
      541 gcaattgaaa aaaagtggca ggatatatgg gaaaataaag gtgtgtttca cgccaaaaat
      601 gattataccc tacccaaatt ttacgcactg gtggagtttc cctatccgtc cgggcagggc
      661 ctgcatgtag gccatccccg ttcctatacg gctctggaca tcattgcgcg caagaagaga
      721 cttcagggct ataatgtcct ttatccgatg ggatgggatg cgttcggcct gccgacggaa
      781 aattttgcga tcaaaacaca catccatccg gagatcgtta caaagaataa tattgcccgc
      841 tttaaacagc agctccggtc acttgggctt tctttcgact ggtcgaggga aatcaatacg
      901 accgatccgg cctattataa atggacccag tggatttttc tgaagctttt tgaaaaaggg
      961 ctggcttata aaaaggaaat ggccgtcaac tggtgtacct cctgcaaggt tgttcttgcc
     1021 aatgaggaag ccgtcggcgg cgtctgcgaa cgctgtggcg gtgaggtgat ccgcaaggta
     1081 aaaagccagt ggatgcttaa aatcaccgaa tatgcccaga gactgatcga cgaccttgat
     1141 gaagtcgatt atattgaacg cgtcaagacc cagcagaaaa actggatcgg gcggtcaacg
     1201 ggagcggaaa ttgatttttc tacgacctgc ggtgatacgc tgactgtcta caccacaagg
     1261 ccggacacgc tgttcggcgc gacttatatg gttatttctc cggaacatcc ggcgatcgaa
     1321 aaatgggcgt ccagaatctc caatctcgat gaaatcaggg attatcagga ggcggcgtcc
     1381 cacaagtccg attttgaacg cacagaggtt cagaaagaca aaaccggcgt tcggcttggc
     1441 ggagtggcgg cggttaaccc ggccaacggg aaagaaatcc cgatttttat ttcagactat
     1501 gtcctggtaa cttatggaac cggggccatc atggccgttc ccggacatga tacccgtgac
     1561 tgggaattcg ccaggaaatt cgacctgccg attatcgagg tcgtcaaagg cggaaacgtt
     1621 gaaaaagagg cctttaccaa ttgtgaaacg ggcgtgctcg tcaattccgg ctttttagac
     1681 ggaaagacgg ttgacgaggc gaaagcggtc atggtcgact ggctgaccag taaaaagatc
     1741 ggccgtcaaa aagtgaacta taaactcagg gactgggtgt tttcccgtca gagatattgg
     1801 ggggagccca ttccgctggt ttattgtgaa aaatgcggat gggtgcccgt accggaagat
     1861 cagcttccgc tcagacttcc ggaagttgct tcttacgagc cgaccgaaaa tggggaaagt
     1921 cctcttgcca agatggagga ctgggtgaat accacctgcc cccgctgcgg aggaaaagcg
     1981 aaaagggaaa ccgataccat gccccagtgg gcagggtctt cgtggtattt cctgagatat
     2041 tgcgatccgc acaatgatca ggagctggcg agcccggaag cgcttaaata ctggctgccg
     2101 gtcgattggt acaacggcgg aatggaacac accacattgc atctgctcta ttcccggttt
     2161 tggcacaagg ccctgtatga cctcggaatt gttccgacgt cggagcctta tcggaagcgt
     2221 accagtcacg gtatgatttt gggtgaaaac ggcgaaaaga tgtccaaatc gcgcggtaat
     2281 gtcgtcaatc cggatgagat tgtcaatgaa tgcggcgctg acaccatgcg tctgtatgag
     2341 atgtttatcg gtgattttga aaaggccgct ccgtggtctt ccgcaagtat caaaggctgc
     2401 cgcaggtttc tggagagagt ctgggcactt caggatgttg tcacctcgca ggaggattat
     2461 agcagggagc tggaatcgtc gttccataaa acagtcaaaa aagttaccgg cgatatcgac
     2521 catctgaaat ttaatacggc gattgccgcg atgatgtcgc tgatcaatga gatctatgat
     2581 aaaggcagcg tgacggcggg agaatttaaa accctgctgg ttcttctaaa cccctttgct
     2641 ccgcacataa ccgaggaact gtgggaaaca atgaattacg gcgggatgat caccacacgg
     2701 ccgtggcctg aatatgatga aagcaaatgc aaggatgagt cggtcgagat cgccgtgcag
     2761 atttgcggaa aactgaaagg caaggtgacg attccggctc aggcatcgga gtcggaggcc
     2821 ttccgggcgg caacggatct tcagaaaatt gatgcgctga ttcaggggaa aaatattgtt
     2881 aagacgattt atgttcaagg taagctcatt aatattgtcg cgaaataaac cgataaatct
     2941 cgcaacaatg aggccgatac gcttgacaga tacagacaat gcattgtata ataaaatata
     3001 ttaaattttt gatatgatac ttgttacttg tttacatcta agcagaacta accgttttat
     3061 ccctgcttgg tagacggagg gagggaatat atagtggccg aaataagaat caaagataat
     3121 gagtctcttg acagtgctct tagaagattt aaaaggcagt gcgcccgagc tggcgttctg
     3181 gctgaagtca ggaagagaga gcattatgag aagccctctg tcaaacgcaa aaagaagtcg
     3241 gaggcagccc gtaagcgcaa gcactaaact gcgtaaaggc tgaaaagaga acttgaaagc
     3301 gggccttgtg cccgcttttt gtattatcac ggccggtttt tggacaattc gggcgggatg
     3361 gacaaacgaa acggctcgtc ccaaagccgg cttggaggtt gtctggtaga aaagtaggtg
     3421 gaaggtgtct gcatgtggtt ggttccggat aagatttttg aaaaaatcag tgatatagat
     3481 cctgctttca ttgatagcca gggagtaaag ggcctggtgc ttgacatcga caacacgatg
     3541 gcccccaaac atattccgct tcccgatgaa acgctgaaaa attggatcag aaatatacaa
     3601 agtgcgggaa tcagcctgtt tgtaatttcc aataatcgaa tgaacagggt ttcccggttt
     3661 gcaaaagctc ttgagcttcc ctatatttac agcggcatga aaccgttccc ccattcgttt
     3721 cggaaggccg tcagagagat gaatctcgcc cctgaagagg ttgcggcggt tggcgaccag
     3781 atttacaccg atgtcatggg agcccgcctg acgggcatta aggcctggct ggtgtctccg
     3841 gttgagccca atgagagttt ctttttccgg ctcagacgcc ggttcgaggc tcctgtggtc
     3901 agacgctatt acaccggaag ggaagaaaaa aagaaatgaa cccttttttt gggcccgcgg
     3961 gcagtgatga cagatttgca gctcagggct atcggtctgt tatgcaggct ccggaatatc
     4021 tatcccgaat gggtcttgac gcttatgagt atcagggcgg caggggtatc cgaatatcgg
     4081 aagaaaacgc tgtgcagttt ggtaaaaagg cgcgagaatt ttccatccgc ttatccgttc
     4141 atgcgcctta ctatatctcg ctttccggtg tcgacgaaat gaaaagggcc ggcagcgtcg
     4201 actatatact tcagaccgcc agggcggcaa aatggatggg ggccgggcgt attatcgtgc
     4261 attccggctc ctgcgcaaaa atcagccggc tgcaggctct cgaatatgcc aaagatacac
     4321 ttcgcaaggc gctgagcgct ctggatgaac agggcctttc cgatatccat atctgcccgg
     4381 aaacaatggg aaagatcaat cagcttggaa cggtggagga agtaatggaa ctttgcagca
     4441 ttgatgaaag gctgattccc tgcatcgatt tcggccatgt caatgccaga accaacgggg
     4501 gcctcaagtc aaagagggat tatcttaaag tattagaggc ggtggagaac aaattgggag
     4561 gggaaaggct gaggcatttc cattcccatt tttcaaagat tgaatatacc gaaaagggcg
     4621 gggaaaagcg ccatcttact tttgacgacc gggaatacgg accggaattt gagccgctgg
     4681 ccgagctttg ctgccaaaaa ggctgctccc cggtttttat ctgcgaatcc agtggaacgc
     4741 aggctgagga tgcgctgagg atgaaaaaaa tctatcagga tgttgccgaa gggaatgaat
     4801 tttgatgtcg aaacacaaaa tactggttat tcacgggcct aatttaaatc ttctgggcga
     4861 gcgcgaaacg ggaatctacg gaaaagattc cacagagcac atcaacaggg aaattgttga
     4921 cagcgccgat gagcttaatg tagagtgcga aattcaccag tttaacgttg agggcgatat
     4981 cattaacatg ctgcatacgg ctcgtacacg gtttgacgga attattatca atgccggagc
     5041 ctacacgcat tacagcattg ccatcaggga tgcgatcgcc gccatcaaga taccctgcat
     5101 cgaggtccat atgtccaatg tttacgcaag agaagaattc aggcatcatt cggttctggc
     5161 tccggtctgt gtgggccaga tatccggttt tgggaaaaac agctatcttc tggcgctgcg
     5221 cggtctgatc gacataattt agcgtaatgc gggcttttcc taatcctgcc gtgagggaag
     5281 gctgtcctaa aatatgggcc gcaggcggca agcgtttgac gacgcgcggc gagaggaatg
     5341 gtggaaatga aatatattga gatgcttcag cagtccctgc cggacggaat ggacggcgta
     5401 atcatttttt ccgagcataa caggcggtat tttaccggct ttgattcatc ggccgggctg
     5461 ttgcttgtga caagagatgc agcctgtttt ttgactgact tccgttatat tgaagctgcc
     5521 aaaacacagg ccgcaggatg tgaagtgagg cttctggagg atcagcccaa acagcttccg
     5581 atgctttttg atgagtttaa ggttaagacc tgcgggttcg aaacacggga tcttccgttt
     5641 gctgactata taaacctgaa aaaagacctc cctggcgttg aatttaatca aaccactatc
     5701 gctgacgaca tcattgagcg cctgagaatg gttaaatcgg atgaagaaat cgcattgatt
     5761 aaaaaatcac aggagattac ggacgctgcc ttccgccatg taattgaaat gattaaaccc
     5821 ggtgttgccg aaagggatat tgcgcttgaa atcgaatttt ttatgaagaa aaacggtgca
     5881 agcggcccct cgttcgacct gattgtcgtt tccggcgaaa agtcctccct gcctcacgga
     5941 gtccccggag aacggaaatt tcagaacgga gacttcatta caatggatat cggcgctttg
     6001 tatcacggct attgttccga tatgacgcgg acagtggcgc tcggctcgct gtcggatgaa
     6061 cagaaaaagg tctataatct tgttctgaaa gcccagttgg aggcggaaaa agcggttcgc
     6121 ccgggaatcg cctgcaacga agttgacaga atagcaagag atatgatcta cagcgcgggc
     6181 tataccggct gtttcggtca cggtctgggt cattcgaccg gcctccagat tcatgagcag
     6241 ccacgctttt cgcccacatg ccgtacgata ctgcgcaaag ggatgatcat gtcggtcgaa
     6301 ccgggtattt atctggcggg ccggtttggc gtccgaatcg aagatcttgt attggtcagc
     6361 gatacgggct gtgaaatact gacacaaagc ccaaaagagc tgatgatatt ataaatttcc
     6421 ggccaaacga aataaaaccc ttgcaaaatg ttagattata agataaacta ttttttggtt
     6481 atcttgtcgg ctgaccgtcg aagaaagccg attgatcctg tcagcaaaag aatccaattt
     6541 ctgtcggcta accggcggaa ggcccgaaaa cttaaaaaat ataagggtgt ctgtcggccg
     6601 gcgcgcaaca agcgtcggac tgcggctggc tcttgtagga ataatggagg atgaaccgat
     6661 gttatctgca ggcgattttc gaaatggtct gacttttgaa atggacggaa atgtattttc
     6721 agtcgtagaa tttcagcatg taaaaccggg taagggagcc gcgtttgtac gcactaagct
     6781 caaaaatgtt ataaccggcg ccgtagttga aaaaaccttt aacccttccg ataaataccc
     6841 ctctgccttt gttgaaagaa aagatatgca gtatctttac agcgacggcg agctttatta
     6901 tctgatggat aaggaaacat atgagcagat gccggttaac gccgaaaccc tcggcgataa
     6961 ttttaaattt gtaaaagaga atatggatgt caaggtcttg tcttataaag gcaacgtatt
     7021 tggaatcgaa ccgccgttct ttgttgagct tcaggttgtt gaaaccgatc cgggtttcaa
     7081 gggcgataca gctaccaacg cgacaaaact cgccaaactt gaaacgggcg cgagcatcaa
     7141 agttcctctt tttgtaaacg agggcgatat gatccgtctc gataccagaa caggcgagta
     7201 tatggaacgc gcataatgcc aataataact taacgacagg ttatttggct cagatcttaa
     7261 ttttccggtg cataaccgtc ggcgggattt tttcgccgcg ggtgcgttaa ataggattca
     7321 attcaaagga aactgaaaat cagacagagg aggcgtaatt atgactgtaa cggaaaaagt
     7381 tgcatatctc aagggccttg ttgaagggct gggcgtggat gaaggaacaa aagaaggccg
     7441 cattatcaag gcaattgtcg atgtgcttga cgacatggcg ttaagcgtaa ccgatatgga
     7501 ggacaatctt tccgaaattt cggaacaggt tgacgctgtg gacgaagatc ttgaagacct
     7561 ggaaaaagat ttttacggcg acgaggatga agacgacgag gatgaggacg acaccgatta
     7621 ttatgaggtg acctgcccga attgcggcga aaaagtctat ctcgacgacg aactgttatc
     7681 cgacggcaaa atgaattgcc cgaattgcgg cgaaaagctg gagtttgatt tcggctgtga
     7741 ctgctgtgat gattgcagcg gccacgacga tgaggaagac gaataaattt tggtgattga
     7801 ataaagggcc ggagttgata actccggccc tttattttac gtatttactt tttatcagtc
     7861 gttgcagcag ccgtcgtcac cgccacagca gcagcaaatc agaattatga taagtataat
     7921 ccaaatgcag ctgttgttgc cgcggccccc aaagaagttt gatccacaag acatattcaa
     7981 agacctcctt tcatccttac aacaatatac tatggcaaaa gcttgttcgt ggttacttaa
     8041 ttgagaataa tcagaaatca atcgacatat aatattgcta accttgtcag aacttttctg
     8101 tcctgtttga aaattctgat tttaacgaga gcgcaatacg gtttttaccg cgagtaaaag
     8161 cttgatgaaa ttgggaggaa atcgtatgct gtttggagat aagccgagat atttcccgat
     8221 tgacatatcg gatgaaaccg atctgaatga tcttgatgcc gaacaggagc ttctgccttt
     8281 ccatcctatg tattccggaa caaaagtgtc aaacaaaaaa acagtaagat cgagaaaaaa
     8341 attaaaaaat aaagagagtg aaagaaaaca ggaggaaaat taatataatt ccgaatttct
     8401 tttgaaatta gactttaact gactttgacc ataataggta aaagaatata ataagacata
     8461 gaaggtcaag gaaagtcaaa ggcaaagtga gaggagaaca tccggtgaaa ttcagcgatc
     8521 tgatcacaga aatgattttg cggatgctaa gcgaagcaga cggaatcgtc gaaattcaga
     8581 gaaacgagat ggcgaaccgt ctgggatgtg ttcccagcca gatcaattat gttattacct
     8641 cccgatttac gccagagcaa ggttatttcg tcgagagtcg gcgcggcggc gggggttata
     8701 tccgaatcac cagaatcaaa tatgaccggg accagatgct gatgcatatc gtaaacagta
     8761 ttggagaccg cattgatgcg gaatcggccc gaatctttat tgacaatctc agtgacagcg
     8821 gtatagtcgg tgaagaatcg gcccggctta tgagggcgtc tctctccaat acggtttacc
     8881 gcgttgtgcc gcaggagatc aaagacaatc tccgggccga gatctttaaa agaatgctat
     8941 tatctttact gaaataagga ggtttcagct atgctatgcg atcgttgtgg aaaaaatgag
     9001 gcgacgactt atctcaagga ggtcattaac gggaatgtca aagagctgca cctctgcagc
     9061 cgctgcgcgg aacagctggg gtatgataac gtcttcggaa gttttaatcc ctttgatatg
     9121 cttggggtaa atgtcggaaa tttccttggt gggttctttt cccagtcact gccggagcag
     9181 acatccgaaa gctctaaaaa atgcagtttc tgtggaacaa cctttgaaga tcttgcgaag
     9241 acggccaagg ccggctgtgc gaactgttac agcgaattct atcaggaaat tctgccctct
     9301 cttcagagaa tacacggaaa gacccgtcat gtcgggaaaa tccccgggcg agcgggaaaa
     9361 gaactcaagc tccgcagaga attaaatacg cttaaacaga agctgaatga tgctgtcagc
     9421 gcccaggaat atgagcaggc agctcagctg cgggatgaga tcaaagagct tgaaaagaag
     9481 gtgcagggag aatgaaatgg tatctggaag tcgggccgga gggcgacgtt gttatcagta
     9541 cgagaatcag gctggcgaga aatgtggcgg aatatccgtt tccctgtaaa atgacggtgg
     9601 agcaggggaa aaaggttgcc cagctcgcca gagcatcgct gaacggttcg gaaaactatg
     9661 aatttattga tatgcagaag cttaccccgg caagagcaat gtcactcgcg gagcggcatt
     9721 tgattagccc ggaatttgcc cagaacagag atggaagggc gctgctgctt tcagaagatg
     9781 aatcggtgag catcatgcta aatgaggagg atcatatccg gattcagacc atgttttccg
     9841 gtatgcagct ggaaaaggct ttggaagccg ccggaaacat cgacgatatc atggatgcga
     9901 aactaaatta tgcatttaat gaaaatctgg gctatctgac ccaatgcccg accaatctgg
     9961 gaaccggtct cagagcaagt ctgatgctcc atttgccggc gattgcgcag tcgggcgcca
    10021 taccgaatct tgccaacgcg gtttccaaac tgggattcac cgtccgggga acttacggcg
    10081 aaggcagcga ggccaaagga gcatttttcc agatttccaa tcagattaca ctcggcttgt
    10141 ctgaggagga agccatccgg aatcttaaag atgttgccgg ccaaattatt tcacaagaga
    10201 gggcggccag ggaaaacctg aaaaagcttg gtcctcagct ggaagacagg gtctggagaa
    10261 gttatggagc cctcagccag gcaagaatgc tttccgccga cgaatttatg tcgctgattt
    10321 cagatgtcag gctcggagtt acggcggggc tgattcaaaa cctcaagctt gaagaaattc
    10381 atctgctgat ttatgaagtt cagcctgcgt cccttgtcgc ggctgccggc cgaaatctgg
    10441 acgcgcaggg cagagatgtg ctcagggcgg gaattgtcag agaaaaaata aggtaatact
    10501 atattacagt acgacagtat tttcaatcgt ttttgttcta gaatagtcat actgaacgct
    10561 gtaataccgt gtaaaacaca ttggaaccat ctaaagttca aacatgcgtt actattttga
    10621 aaaaagtatg gcatgatgtt ttaaatttgt tgaaggcatt atgaaatcgg aacggaggga
    10681 atcagccatg tatgaaaaat ttacagaaaa agccgcggag tcgctgggtc tggccatgca
    10741 ggcggcaaga gagatgggcc acagttatat cggcagcgag catttgctgc tggggttgtt
    10801 aagagagggc agcggagttg ccgccaaggc tcttgaggca aaccatatct catccgatga
    10861 agttgagaaa tttatttctg agctgatcgg caggggagag ccgtcgaatg tcagcccggc
    10921 ggatctgacg ccgcgcggca agcgaattat tgagcttgcc tatctggagt caagaaatct
    10981 cggaaacaat tacgtaggca cagagcatct gctcatggga ctgcttagag aaggagacag
    11041 cgtggcggtc agaattttgg ctgaacttgg ctgtgacccg agagcgcttt atgaagagat
    11101 cgttcgctcg attggcagcg agcctgcgga gccagccgga aaaagcgcca atcttggtca
    11161 ggttaaatat accgggggcc aaaaacaggc ggggaacaca ccaacgctga accagtttgg
    11221 cggggatctg accgctcagg cccgggatgg aagactggac cctgtggttg gccgctcaga
    11281 tgaaatacag cgggtgctgc agattctctc aaggcgaacc aaaaataatc cctgtcttat
    11341 cggtgaaccg ggcgtgggca agacagcaat cgccgagggc cttgcggaaa aaatcgtcga
    11401 gggcagggtt cccgagctgc tgaaagacaa aagggttgtc acactcgata tgtccggcat
    11461 ggttgcggga acaaaatacc gcggtgagtt tgaagagaga atcaaaaaag cgcttgagga
    11521 ggtcaaaaag gcgggaaatg tgatcctgtt tattgatgag cttcatacca tcgtcggggc
    11581 cggcgccgcg gaaggcgcag tcgatgcggc caatatttta aagcccttgc tcgcaagagg
    11641 ggaaattcag gttatcggcg cgacaaccat caacgaatat cgtaaatata tcgaaaagga
    11701 cgccgcgctt gaacgcaggt tccagccggt taccgtcggg gaacccagtc aggaagaatc
    11761 catcgccatt ttaaaagggc ttcgcgataa atatgaggct catcacaaag tgaagatcac
    11821 cgattcggca cttgaagcgg ctgtgaagct ttccgcgcgc tacatcaccg accggttttt
    11881 gcctgataaa gccattgact tgatggatga ggccgcaagc agactgcgcc tgaaagcgtt
    11941 taccgcgccg cctgatctca gagagctgga cgataaactc aaaaagcttt cggaggaaaa
    12001 ggaggcggcg gtcaaatcgc aggattttga gcgggcggca aagctcaggg atgaagaaaa
    12061 atccctgaaa gaaaccgttc agtcccaaaa gacagaatgg aagcacaaaa acgaacgcag
    12121 cgtcaatgaa gttacggaca atgagattgc cgaaattgtt tccagctgga cgggagtccc
    12181 ggttaaaaag ctcgctcagg aggaaagcga ccgtctgctg aaaatggagg agattttaca
    12241 ccagcgggta attgggcagg aagaggcggt atccgccgta tccagagcca tccgcagggg
    12301 ccgtgtcggc cttaaggatc ccaagcgccc ggttggatca ttcatttttc ttggacccac
    12361 aggagtcgga aaaaccgagt tgtgcaaagc gctcgccgag tcgctgttcg gggacgaaag
    12421 cagtatgatc cgcattgata tgtccgagta tatggagaaa cataccatat cgcgcctgat
    12481 tggagcgcct cccggctatg tcggatatga tgaagcggga cagctgacgg agaaagtacg
    12541 ccgcaagcct tattcggtta ttttgtttga tgagatagaa aaggctcatc ctgacgtttt
    12601 caacatcctg cttcaaattc ttgaagatgg tattcttacc gattcgcagg gaagaaaagt
    12661 ggattttaaa aataccgtta ttatcatgac gtccaatgtt ggcgcaagat tgattaccga
    12721 aaaacataaa ctcgggtttg gtatgagcga cgacagtctc acacagagtg acaaagcaat
    12781 caaatccgat gtgatgggcg agctgaaacg tacgttccgc cccgagtttc tcaacagggt
    12841 ggatgatatt attgtctttc atcagctttc cgaggagaac attaagaaga ttgccggacg
    12901 tatgctgaat attctgacca agcgggtggg aacctatgat atttccattg atttttccga
    12961 cgaggtagtc tcaaaggttt caaaagaagg atttgactca gtttacggtg caaggccgct
    13021 ccgaagagcg attcaaaacg aaattgaaga taggctttcg gaagaaatgc ttcagggtcg
    13081 gatcaaggcc ggcgatcagg ttaatacggt tttaaaagag gataaaatca cttttgaaaa
    13141 agtgaaatga ccggcaggtt ggactgccgc cggaacggcc cggcggcagt ctttccgact
    13201 gctatttcac ctttatggaa atagaatcgg ctgttgattt ttttcgtctc ctatgatata
    13261 atcatgggag tcatccggtt accaatcttc aactttagaa tcgcgtagat tctgctctgg
    13321 attgatttca ttacgcggga gtagttcagc ggtagagcgt cggcttccca agccgaatgt
    13381 cgcgggttcg aatcccgttt cccgctccag aggccggcct ttttgatggc cggctgtttt
    13441 gcttttattg ccgtgtccag acaatttatc tgacttttac agataaaaac caatgaatcg
    13501 taatgttttg gttatagtaa gaataattca caaaacaaag ggcgttaatc aattagaatt
    13561 ttaattgatt tctctgaaga ggccgatgct ttgacatgaa aaagggtggt ggcggatggt
    13621 actggaatgc tgggttatta tattgattct tggcatcgcg gcctacatgt ttgtccgggg
    13681 cggccgaaaa atctgggcgt cgggcgtttt acctttgatg ttggttccgt ttgccgacat
    13741 tatctattcc ccgattggca gaaggctggc tcgccacagc gtcgcatact ccaacggtat
    13801 ccgactttcc atttacgggg tgctttttat catcgtatcc atttgggtca tcctgtgggc
    13861 aaaacggctt ccctcaggta aatccaaata cacctatatc attatatcaa tcggttttac
    13921 ccttactttg attctgctgt ttataaagaa tctgcatgtt ttgggttaaa cgcgcggttc
    13981 cggctgtaaa aatggccgcg gaacagtatt ccgcggtttt ttcttttcat aagaggggaa
    14041 aaccggtacg caattttggt gccaatatag attgacaaaa gttaaatttg gtattataat
    14101 tattaacact ttattgaaat atctgcggat gtagctcatc tggtagagcg ccaccttgcc
    14161 aaggtggagg tagcgagttc gagtctcgtc atccgctcca agcataaaaa gagacagcct
    14221 caatttgagg ctgtctcttt tcatgctatt taaaacctct gcggatatgg ttatatattc
    14281 cctgcggacc cggtaaccga ctcttttaaa tacttcaagg cgaaaattta ttgttctaaa
    14341 caattagtat ttcgttttta gcgatgaaat aacttccaat tttcaaaaaa caccttgaga
    14401 gattcccgcg tgatacgaaa atgatccaga ttttccaggc tgatactgcc gtcttctttg
    14461 tgcgtaaccc aaaatcgggg atcataaatt attggcacgc cgtgttttct cgcaagctcg
    14521 gcaatatgat tttcttcaca gaataaaaac atacgttcgt cgaataactt cccaccgaaa
    14581 gtctgcaaaa cactacgcgg caggaggaaa aaacttccgt gaagagcgta aacttgcctc
    14641 ggcccttttg catcgccttt tcgcagcact gctttttcca gtttattaaa caggatggcg
    14701 gctaagaaaa aaggcaggaa gcctgctttt gcgtaaagaa agcgaaagaa tagaacgaaa
    14761 aaattccgac gataatggat cggattttgg tttttgcccc gcagggtaag aatcttaggc
    14821 ccgaccacag cattctccgc cgcggtaagc cgatccagat caaactcacc gattcggatg
    14881 tccggattgc tgacggccag atattgaaac tgataatgct ccagcgcgaa ccgtatgccc
    14941 gcgttgttgc cgcttccgta gcctttgttt tccacgctga aaaaatcaca gccgtatgcc
    15001 tgggcaaccg accggattgc actttcgctc tcattgtcgt agaaactgtt taccacaatg
    15061 acccggtaag aacagccaag ggaagcgagg gaatctaaaa agtcagtaag atccttgtcg
    15121 tttcggtagg tgagcgttac caaaaccagg tcgaaattca tccaatcatc ctttgatccc
    15181 gaaattcagg cgcgcaatcc gattagcgcg ccggacggtt gccccggtga aacaggcaga
    15241 aaaaatcccg taccgctccc cggaaaccgc tgtaaaattt acgatacccg ccgtttaaac
    15301 agtccgaaac gataatggca aagccctgaa aaaagccgcg ggagtcaagg ctcagctttc
    15361 tgttccagaa cgcgatgcag cgtttcaact cggatagatc ctccggcgcg ggcaaccggc
    15421 ccaggtcttc cagccggttc aaaacatcct gatagcggtt cctgcgctgc aggcgaattt
    15481 tccgtacggc ggaaaaatta ccgaggtagc gccgaacccg gctgaaaggg tcgctgctgt
    15541 tcgccccgac aacatttttt tcatgctgcc ggtaagacag caggcattga tccaccggcc
    15601 ggacctccga tagacagctg cagagaatcg ccagccaccc gtcgtgaatc cagaaagggg
    15661 aaagcggcat cgcatccggc agaagcgatc ttcgaaaggc catcgcggtt cctgtgacca
    15721 cacagcggtt gagcagcagc cggaaaatat tttcatattc ttttcccctg ccgaccatga
    15781 aagggaaaaa attgagtgac gaccaaaggc tgcgtcccag aggtctcagc tccgaatcgg
    15841 cgagctccgc gttggaaaaa acgaccgagc agccgggggc gctctcaaaa atatccgcca
    15901 taattttaat tttgtcgggc ttccagacgt cgtcctgatc gcagggaaat agaatgtcgc
    15961 ccgtgcaggc ttcaaatgcc cgggcaaagt ttccaacaac cccgaggttg cggtcacttt
    16021 tcagaatttt tacgcgaata ttgccatggc ttttgaaaga cgcgaggacc tcatccgcca
    16081 gagcaagagt tctgtcggaa gaatggtcgt cgctgatgat gatttcatca ggcagccggg
    16141 tctgtgagaa aaggcttctc agttgttcct ctatataagc ttctccgtta aaggtgccga
    16201 cggcaaccga tattttgcag ctgtttgcca ttttgaagaa ttctccattc acaacagagg
    16261 ctaagtgctc tcaggttaat ttgaacccgt tcttaaacaa gcttaatttc ttgtttccct
    16321 cgatattcaa aaccatcagc gccgcaagca gcaactcagc gagaagggac gctatgcccg
    16381 cgcccaccgc agaataaaat ttgatcagaa taatcgaaga accaagaaga aaaacggcgc
    16441 agaacagaat aatgttccgg aatttgatct ccataccaca gttgaccata atctgagttc
    16501 catagatatt gttaataaaa atagtagtgg ggatgatact cagaatgcgc atgatgcagg
    16561 tgatttccgg gacattgcgg cctgtcatca ggaaggtgat caggccggcc gaagcgaata
    16621 gcgccgccga taccgccaaa aagaaaaaaa cgccgtaggt cagcactcgt tttacaaagg
    16681 ccagtgcctg ctcggttgat ttggcaaaca accgggtcgc gcgtgggtat acagccgttg
    16741 tgacggggct gatcaggctg actacgaggc gcaccgcttt gtcggctgcc gcgtaataac
    16801 caacggctgt attggaaaca aaaaatccga gcaaaaccgg attggcatta ttgaaaatcg
    16861 aagttgagaa ttgggcgaga aacaggttcc agttttcagt catgtagctc ttaagaacgc
    16921 cgcgttcggg cattcttagt ttgacgccgt atttgaaaaa aatcggaaac actgccatga
    16981 ttccggcgag tacgctcgac agcgcttgaa tggcgcaggc gagattcaga tcgcccgggc
    17041 ccctgacggt aatcagaagc aaaaccagcg cgatgagttt cgaaacgaca ttcagcgcgg
    17101 taatatacga catactttcc ataccctgaa aaaaccaggc cgggaataat acttcgccga
    17161 ccagagagag catggtgaag aaatatgcca taaaattaac tctcatgatg ggaatcgaag
    17221 cggcaagcaa tgtgatggtg aaaaaactca ccagcattaa aattgacttt ataataagga
    17281 tggtgctgaa aatctccgaa attttctctt tgttctcccg atggatggcc accagccgtg
    17341 gacccgaaag ataaaatccg aaattgacca gcagcacaaa ataactgatt accgactggg
    17401 ccagcgaaag cacgccgtag ccgcctattc cgatcacccg ataaagatag gggatggtca
    17461 cgaggggaaa aagataattg gtcatttgca gcgcggtcag ataacccata tttttaaaca
    17521 gacggttttc cttgatcaaa ttcattagct tcataaacag actccgattt cttttgtttc
    17581 aaccattcgg ccattcggat tctgagaccg gagattctta gaaaaagtat tttttaaaag
    17641 catgtaaagt gtataggctg aagcaataga ttgatttcag aagtcccaga tgctcagatt
    17701 tgcggtaaat tctccaggta cggcggagag ccgagagctt gttggcggag accgagtctt
    17761 tcaccagacg gtagagcacc agagcttcgt cgatgccaaa ggccagctgg ccttttttta
    17821 gaatcgaaag ccaggtggcg taatcctgac ccttgggaat ttcaggcatc ctaaaatcac
    17881 cggttgccga tctgtcgatg agtaccgagg agcatggtat cacggtgttg gtcagaagat
    17941 aacggtaatc tacaacgggg cgtatttttc gtttgctttt aacctgatgg ccatcttcgt
    18001 cgatcatttc aatggcagtg aagcaaaaac cggcccggga ggcttcgaga aggtgaagct
    18061 gtttttgcag cttcatcggc cgccagacgt cgtcggcatc gagaaaagct acatatcgtc
    18121 cgcgtgcttt cacgagcgcg gcgtttcgcg cgcgggaagc tcccccgttg cggtcgagac
    18181 gttcgtaatg aatcctgccg tcaaatcgtg aatagttctc aatgatctcc tccgaccggt
    18241 cggaagagca gtcgtccaca atcagcagct cccattcccc gtaggactgg gaaagaaccg
    18301 actcgatgga ctgcgcgata tatttttcct tattataaac cggcataata atggaaacca
    18361 accgattatc cagagccata gtgtttctca aaaccttatc gatctatttt gaaagaatga
    18421 aattcttgtg acaattcttg aattatagca taatcaaatt attttttcta ctcaattgta
    18481 agaaaagcgc tttaaaattt acaatttggt tacaatcttc ccaaaccagt gaagtctctt
    18541 tccccattca cctaaactcg gcaaccaagg aggatctgac ggcgctgccg ggtatcggcg
    18601 acacacttgc ccagcgtatt attgactatc gctcccagca cg
//
LOCUS       DATALY010000335         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_677456,
            whole genome shotgun sequence.
ACCESSION   DATALY010000335 DATALY010000000
VERSION     DATALY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677456"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VHR42_09130"
     CDS             <1..716
                     /locus_tag="VHR42_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3027368.1"
                     /translation="TKIYHCDGKKIINGAEVTCLKAHGDVNLKQALAQSCNIAFAEIS
                     LELGGNTLQKYANKAGFNSSLKVDGIQTAMGKVDVAGAEGGDLAWAGIGQYSDTANPL
                     NFMAYVGAIANGGVRITPKLVIEKGIYSAVFGEHLQKKQVLSVETADKIRTMMRNDVL
                     EVYGEDNFKGLELCAKSGTAEVGEGKKPHSWFAGFLDREDCPLAFVVVVENGGTGSKV
                     AGSVAAKVMKEAVYRMTGE"
     gene            893..2143
                     /locus_tag="VHR42_09135"
     CDS             893..2143
                     /locus_tag="VHR42_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3027369.1"
                     /translation="MGGRVSLNFIHTGFPALRHRNFRLFWFGQCISLIGTWMQNIGQS
                     WLVLELTNSALKLSIVTTMQFLPVMIFSLYAGTLVDRLPKRKVLLCTQSALAVLAAVL
                     ATLTYFKVVEYWHVLILALLLGTVNTLDMPTRQSFVIELAGKEDLPNAIALNSSIFNL
                     GRIIGPAVAGLLIGLIGIAPCFYLNAVSFLAVIANLWMIHVPSKAAKEKEFGSLERVY
                     LDIREGLSYINQKKIIKRPLLLLAFISTFVMNYNILVPVFAQQELNQNATGYGLLMTG
                     MGIGSFTGSLAMVAGSKKNPKLRYLIAGAFAMSAFLVLLAPEKSFRLACVTLFFIGFF
                     SIIFTTMVNTTIQLNSSDDMRGRVMSVYTLVFGGVTPIGSLFTGQVTEYAGAPGCMLI
                     SGIIGIFAAACTAFITWSSRQTSK"
     gene            2163..3023
                     /locus_tag="VHR42_09140"
     CDS             2163..3023
                     /locus_tag="VHR42_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3027370.1"
                     /translation="MGRDSKNTAVLYMMLCAVLWSTAGILIKFLPWNPMVIAGVRSLI
                     SAGVYLLYMRYEKTEFKINTYSILSGAALMCTFLFFIAANKFTTSANAIVLQYSAPVF
                     ILILSALIYHQKFRAGDVITVAATSVGISLFFLDKLSGGYLFGNLLALIAGLSFASMF
                     VITGHADDDSRSSGILLGHIFTAIIGVPFIFFSTTPVTPPIVAVILAMGIFQLGIPYI
                     LYGLAVRKCSPLACSLISAIEPLLNPVWVFLYNGEAPSLFALIGGAIVIGAVVAWSVW
                     SSKAETSMSE"
     gene            complement(3080..3256)
                     /locus_tag="VHR42_09145"
     CDS             complement(3080..3256)
                     /locus_tag="VHR42_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX3027371.1"
                     /translation="MNYADAAEIDSWGLEGVRFVTENGLMTGTSGNQFEFDGPSTRAQ
                     VAVVMERLLNLTGE"
     gene            3278..>3529
                     /locus_tag="VHR42_09150"
     CDS             3278..>3529
                     /locus_tag="VHR42_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX3027372.1"
                     /translation="MAYENKIASGMGDSLFAPDKPVTRGEMAVIITNYMKFIGKGTAG
                     TAALNYTDAAEIDSWGLEGVRFVTENGLMTGTSGNQFEFD"
BASE COUNT          835 a          827 c          922 g          945 t
ORIGIN      
        1 atacaaagat ctaccactgt gacggaaaaa agatcatcaa cggtgcagaa gtaacctgcc
       61 tgaaagccca cggcgacgtg aaccttaagc aggccctggc ccagtcctgc aatatcgcgt
      121 ttgcggaaat ctctcttgaa ctggggggga acacgttgca gaaatatgcc aataaggcag
      181 gttttaactc cagcctcaaa gtcgacggga tccaaaccgc aatgggcaag gttgatgtgg
      241 ccggcgctga gggaggcgat ctggcgtggg cagggatcgg acagtacagc gatacggcaa
      301 atcctttgaa ttttatggct tatgtcggag caatcgccaa cggcggcgtt cggatcacgc
      361 cgaagcttgt aattgaaaaa gggatctatt ccgctgtatt cggagaacat ctccagaaaa
      421 agcaggtact ctcggttgag acagcggata agataaggac aatgatgcga aatgacgtac
      481 tggaggttta cggagaagac aacttcaagg ggctggagct ctgtgccaag tcgggaaccg
      541 cggaagtagg ggaaggcaag aagcctcact cctggttcgc agggtttttg gatcgcgaag
      601 attgccctct ggcctttgtg gtcgttgtgg aaaacggagg aaccggcagc aaggtcgcag
      661 gatctgtggc tgcaaaagtc atgaaggaag ccgtgtacag aatgactggg gagtaaatat
      721 caatattgag cggttgataa atttcgaaat gatttattta tttgatttga aaggcagttt
      781 ttcggtataa tataatagga cagaagaaaa taaaaatttc cagtctgttg atgctgtcgt
      841 cccggcagca ttattatttg tgtatgttaa agtgttttaa gcgtaggttt caatgggcgg
      901 cagagtatct ttaaatttca ttcataccgg ttttcccgcg ctccggcaca ggaattttcg
      961 gctgttctgg tttggccaat gcatttcatt gatcggaacc tggatgcaga atatcggtca
     1021 gtcctggctg gttctggaat tgaccaattc ggctttgaag ctgagcatcg ttacgacgat
     1081 gcagtttctc ccggttatga tcttctcact ttacgccggc acactggtag accggcttcc
     1141 aaaacggaag gtcctgctgt gcacccaatc cgcacttgcg gtcctggcgg cggttcttgc
     1201 cacgctgacc tatttcaagg ttgttgagta ttggcatgtg ctgatccttg ccctgcttct
     1261 gggcacggtg aatactcttg atatgccgac acgccagtcg tttgtgatcg aactggcggg
     1321 gaaagaggat ctgcctaatg cgatcgcgtt gaattcaagt atattcaacc tcggcagaat
     1381 catcggccct gcggttgcag gcctgctgat cggactgatc ggaatcgctc cctgctttta
     1441 tctcaacgcg gtcagcttcc tcgctgttat tgcaaacctc tggatgatcc atgtgccttc
     1501 gaaggccgcc aaagaaaaag aattcggctc gctggaaagg gtatatctcg atatccgcga
     1561 gggattgagc tacattaatc aaaagaagat cataaaacgg cccctgcttt tacttgcctt
     1621 tatcagcact tttgttatga attacaatat tcttgttccg gtattcgctc aacaggaatt
     1681 aaatcaaaac gccacgggat acggcctgct gatgaccggc atggggatcg gctcctttac
     1741 cggctcgctg gctatggtag caggaagcaa gaagaatcca aaactcagat atctgatcgc
     1801 aggcgcgttc gccatgtcgg catttctggt gctgctggct ccggaaaaga gcttccggct
     1861 tgcctgtgta acattatttt tcatcggctt cttttcaatc atctttacta ccatggtcaa
     1921 cacgacgatc caactgaatt cttcagacga tatgcgaggc cgggtaatga gcgtttatac
     1981 gctggttttc ggaggggtga cgcccattgg cagtctgttt accgggcagg tcacagaata
     2041 tgccggagct cccggatgca tgcttatcag cggaatcatc ggaatttttg cggcggcctg
     2101 cactgctttt atcacgtgga gcagccgtca gacttcaaaa taattacaaa ggcaggaata
     2161 aaatgggacg agattcgaaa aatacggcgg tactttatat gatgctatgt gcggtgcttt
     2221 ggagcacggc ggggattttg atcaaatttc tcccctggaa tccaatggtg atcgcaggag
     2281 tcagaagtct gatttcagcg ggtgtttatc tcctctatat gagatatgaa aagactgagt
     2341 ttaaaatcaa tacatattcc atcctgagcg gcgctgccct tatgtgtacg tttctgtttt
     2401 ttatcgcagc gaataagttc acgacatcgg ccaatgcaat tgtgctgcag tattcagcgc
     2461 cggttttcat tctgatttta tcagctctga tttaccatca gaaatttcgt gcaggtgatg
     2521 tcattaccgt tgctgccact tcggtgggca tttccctgtt tttcctggac aagctgtctg
     2581 gaggctacct gtttgggaat ctgcttgcgc tgatcgccgg actgtccttt gcttccatgt
     2641 ttgtgatcac cggccatgcg gatgacgatt caagatccag cgggattctc ctcggccaca
     2701 tttttactgc tattattggg gttccgttta ttttcttttc cacgacgcct gtcacgcctc
     2761 ccattgtcgc cgtgatcctg gcgatgggaa tttttcagct gggaattccc tatattctat
     2821 acggccttgc cgttcggaag tgctctccgc tggcctgctc cctgatcagt gccatcgaac
     2881 cgctccttaa tccggtctgg gtctttcttt ataacgggga agccccgagc cttttcgctc
     2941 tgatcggcgg agccatcgtg atcggcgcag tggttgcctg gtcggtttgg agcagtaaag
     3001 cggaaacaag tatgagtgaa taggaaaggc ggctgcttta cgcagccgcc acttttttgc
     3061 aggatagttc tatgtattat tattctcccg tcagattcag gagcctctcc attactaccg
     3121 caacctgagc cctggttgaa ggcccgtcga attcaaattg gttgcctgag gttccggtca
     3181 tcaatccgtt ctcggtcaca aaccggactc cttcaagtcc ccagctgtcg atttcggcgg
     3241 catccgcata attcaaagct gctgtgccgg cggttccttg gcctatgaaa ataaaatcgc
     3301 aagcggcatg ggcgacagtt tgttcgcacc tgacaaaccg gtcacccgtg gagaaatggc
     3361 tgtgatcata acgaactaca tgaaattcat aggcaaagga accgccggca cagcagcttt
     3421 gaattatacg gatgccgccg aaatcgacag ctggggactt gaaggagtcc ggtttgtgac
     3481 cgagaacgga ttgatgaccg gaacctcagg caaccaattt gaattcgac
//
LOCUS       DATALY010000336         7168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_503532,
            whole genome shotgun sequence.
ACCESSION   DATALY010000336 DATALY010000000
VERSION     DATALY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503532"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..498
                     /locus_tag="VHR42_09155"
     CDS             295..498
                     /locus_tag="VHR42_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HEX3027373.1"
                     /translation="MNGQIRNNIKIGALVDIVLKKDQPTGKLTRGHVKRILTNSPTHP
                     HGIKVMLEENDMVGRVQAICEKG"
     gene            707..1069
                     /locus_tag="VHR42_09160"
     CDS             707..1069
                     /locus_tag="VHR42_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3027374.1"
                     /translation="MILNSLTASIRKNKTGMLIMVFSALFSSFGQFLWKEFEHTGGGH
                     YLFLLAGFALYGLGAVCMIAAFRFGSFSVLHPMQSLSYIFALIIGFAFLNEAITATKA
                     VGLAIILFGVVMIGVGDE"
     gene            1062..1400
                     /locus_tag="VHR42_09165"
     CDS             1062..1400
                     /locus_tag="VHR42_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3027375.1"
                     /translation="MNKLILILVIIFMTMLGSLGGFFFKKSTGSESMFATLKNKYLYI
                     GGVLYVVAALINVWVLKFLDYSVVMPMTAITYIWTLIVARVALKEKLTGKKVIGILAI
                     ITGALFIGLF"
     gene            complement(1664..1906)
                     /locus_tag="VHR42_09170"
     CDS             complement(1664..1906)
                     /locus_tag="VHR42_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cortexillin II"
                     /protein_id="HEX3027376.1"
                     /translation="MERKRKTVETQITEIEAKKAQYQAKINNYKAKISDLDSKIRDLK
                     DIQKQKELENLLDVIKTSGKTPEEVIAAIKIGNVQN"
     gene            complement(2314..3183)
                     /locus_tag="VHR42_09175"
     CDS             complement(2314..3183)
                     /locus_tag="VHR42_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX3027377.1"
                     /translation="MAKIEVFADSSCDFPREYAAAMHISIVPLTVSFGNRQFTDGVDI
                     DTNTFFRLLRENPSVIPKTSAPSPDEFMKAFLGVGDDCEKIICVTVTSKSSSSYNSAV
                     IAKNILNEDPGFKPQIIVFDSLNASLAIGFIASEATALAQQGEAFDKILLRLNQMRNR
                     TSIYFILDTLEYVRKGGRIGAIKAVLGSLLNIKPILTFRNGTPADVDKTRGFPQAQLK
                     LVKLFTDKAISRDEVYIIHAASKKHAEELADTIQRLAPNICIHIFEVGAVMGTYTGPG
                     GIGLVFEEKFNKQ"
     gene            3431..4384
                     /locus_tag="VHR42_09180"
     CDS             3431..4384
                     /locus_tag="VHR42_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX3027378.1"
                     /translation="MATTTAAAFTPDMSKPVEIKEMEKVQAMDLKRTSRILFNFSLVT
                     VFLLIFCFLILPDSAEGNGTRAASAESSSGKEQSILEQSRAVTSWGRIVYGKSGLGAN
                     LVCYQICPEGPIKRNILMTFEVHGYEDLFPGDGKALVNMGNSVADYFIKNRGQLQNCR
                     LFLVASANPEGLSHGKSKDGIGRCQASLGVNINRDFDFNFIVFNESRDHTLSAPFAAP
                     ESRALRNLVNAVRPDIVIDCHGWENHFIGDHWLAGFFRDSLGISRINSFDQSQHGYFS
                     AWAGSKGIQSMLIEYPTNAARNSGLYAGKTITGIRRLVGSL"
     gene            complement(4389..5747)
                     /locus_tag="VHR42_09185"
     CDS             complement(4389..5747)
                     /locus_tag="VHR42_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3027379.1"
                     /translation="MVSNIEVNLTEGSVTKKLAVFAAPFFAANFLQAMYGAIDTAIVG
                     WFSNAGGIAAISIGSQVMQIMISLVTGITLGGTILIAQYLGAMQKQDLKELLGTMLTI
                     FFILAVVLTALMGAFTIPILNCLNTPPEAYAQAKAFVYISSAGTVFIVGYNAISAIMR
                     GFGNSKQPLIFIAIACVTNILLDFLFVGFFRLGPAGAAISTVTAQAASMVLSILYLKK
                     NDFIFDFKLKSFRIQKDKAAALLKLGIPVCAQETMINFSFLFITAIVNSFGVVASAAV
                     GISDKFITFAMLPASALSGAIASMTAQNMGAGYHDRARKTMRVGILFSVAFALVFFLW
                     VQIIPETVLHLFRADAAVIADGVVYLRAFSYDFIMVSFVFCMDGFVNGCGHTTFSLIN
                     GLVSSFFVRVPLAFLLSKVLRDGLFGVGVAAPAASVISIIIALIYIRSGHWQKSKIIA
                     KK"
     gene            6093..6995
                     /locus_tag="VHR42_09190"
     CDS             6093..6995
                     /locus_tag="VHR42_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3027380.1"
                     /translation="MGPVVTIKGGDYVFESDYKNRPEIRRSFNCLTEKTFEFSLEQWY
                     QDGYWHENYIPYSLLKNGAVVSNVSVNLMDFQLSGKKKRYVQIGTVMTDSEFRERGLG
                     RYLMEKVLEEWIERCDQIYLFSNDSALAFYPKFGFEKADQYQCSRKVYRKGSSISYRK
                     LEMSSKEDRRLFEKAVSQSKPSARLALLNPLSIVGYYCGSVMRDHILYLDALETAVIA
                     GVKGATIYLHDLFSPHGISAERVLEALYTRNTRKVVLGFTPIDKRLYDENLLQEKDNT
                     LFVLSGKENPFKTHRLMFPVLSRA"
BASE COUNT         2018 a         1550 c         1680 g         1920 t
ORIGIN      
        1 tttcgattat aatcaatcct ctacatgtaa acaaattacg aaaccatccg cgcccgatct
       61 tagatcgggc gatatctgta tcatcattat aaggcggtgg ccgctatgcg tcaatcatac
      121 cgaaagaccc ttaaacggct gcgccgggaa agaaatctgc ttaagcgttg actttttttc
      181 ggaatggaat tatgattttg aaagggagat gccaaaaata aaattataga aatacaggga
      241 aggttttttc tcggctcgat aaagcaaata ggttttcaga aaggagtctt tcttttgaat
      301 ggtcaaatca gaaacaatat aaaaatcggc gccttggtgg atattgtgct taaaaaagac
      361 cagcctaccg gaaaactgac ccgggggcat gtgaagcgta tcctgacgaa cagcccgacc
      421 catcctcatg gaatcaaagt aatgctggag gaaaacgaca tggtgggcag agtgcaggcg
      481 atctgtgaga aaggataaat aaccgggtgc ggatggtttc gtaatttgtt tacatgtaga
      541 ggattgatta taatcgaaaa aaatgctaag ataattgtac tttattgtca aacaaatgtg
      601 caaatgtttc atgtttcgtt tcgcaatacg attccgtatt tttacctcca gggtgacgcg
      661 cgcatcgcgg agctgtctta ccccgacatc tgataggaaa ggtcttgtga ttttgaacag
      721 tcttaccgcc tccatacgga aaaacaaaac cggcatgctt atcatggttt tctcagcact
      781 tttctcttcg ttcggccagt tcctctggaa ggaattcgaa cataccggcg gcggccatta
      841 tctgtttctg cttgccggat tcgctcttta tggtcttgga gcagtctgca tgattgcggc
      901 ctttcggttc ggcagttttt ccgtactgca tcccatgcaa agcctgagct acatttttgc
      961 gctcattatc ggtttcgctt ttttaaacga agccatcact gccacaaagg cggtgggact
     1021 ggccattatc cttttcggtg tggtcatgat cggagtcggc gatgaataaa ctcatattaa
     1081 ttttagtgat catttttatg acgatgctcg gctcgctggg cggatttttc tttaaaaaaa
     1141 gcacgggcag cgaatcaatg tttgccacct taaaaaacaa atatctctac atcggcggcg
     1201 tattatatgt tgttgccgcg ctgatcaacg tctgggtgct gaaattcctt gactattcgg
     1261 tcgttatgcc tatgacggcc atcacctata tctggacgct catcgtcgca agggtggcgc
     1321 tcaaggagaa gcttacaggc aaaaaggtga tcggtatttt ggcgatcatc acaggggcgc
     1381 tgtttatcgg actgttttaa taagacggag atgcttaaag tttttgggct cttcatccgc
     1441 atcctgaata tcaaagagca aatgaaactt tcttttctca ttcaatataa agagaccgtc
     1501 tgttttaaaa acagacggtc tcttgctgtt cacagcagta tcgctggtag cttcgcccgt
     1561 ggaatcgcgc cgacagatca acattgtcga acaaaaatcg gttgacgata ccaaaacaag
     1621 caaaaaaatt agcctgcaat aagcaggcta aaagaatcat gtactaattc tgaacgtttc
     1681 cgattttgat cgccgcgata acttcttccg gcgttttacc cgacgtttta atgacgtcaa
     1741 gaagattttc cagttccttt tgtttttgaa tgtcttttaa atctctgatt ttcgaatcaa
     1801 gatcggaaat tttcgcttta tagttgttaa tcttagcctg gtactgcgct ttttttgctt
     1861 cgatttctgt aatctgagtt tcgaccgtct tgcgttttct ttccaaaata atcactccag
     1921 acttttatca ttaatataat tatagcatta aaaatgaaaa tgtataccac ttgggttacc
     1981 tattctcaat atgtttacaa atatttaaga attacagatg aaagcggata aaatatttga
     2041 atgtaaagaa tgatttcatt tttaaaaaca aaaaatgata gacttattgc ggctgcagtt
     2101 tctgctgaaa aagttacctt taaaaatttt acgatgatcc cggtgcgttc caactcatca
     2161 aaagtccctt caaaataaat aaatgagaag gatttttttg ctcggaaggc tttgcagcgt
     2221 ccttttccaa tctgttcttc ggaataaaat atttttattg acacactgtc gggtcaaaac
     2281 aacaaattat caggacgttg gtggatgtgc agattattgt ttgttaaact tttcttcaaa
     2341 aacaagcccg attccgcctg gaccggtgta agtacccata acggctccaa cttcaaatat
     2401 gtggatgcaa atattggggg caagacgttg gatcgtgtcc gccagttctt cggcgtgctt
     2461 ctttgacgcc gcgtggataa tataaacctc atcccggctg attgccttat ccgtaaacag
     2521 tttgacgagt tttaactgag cctggggaaa tccccgggtt ttatcaacgt cggcaggggt
     2581 tccgtttcga aaggtcaaaa ttggtttgat gtttaataag gagccgagga cagcttttat
     2641 ggcgccgatt ctcccgcctt tgcgaacgta ttccagcgta tccaatataa aataaatgga
     2701 agttctgttt cgcatctgat tcaaacgaag taaaatcttg tcaaacgctt ctccctgctg
     2761 agccagagcg gtcgcttcag aagcgatgaa cccgatggca agagaggcgt tcaaagaatc
     2821 aaatacgata atctgtggtt taaaacccgg gtcctcattc agaatatttt tggcaatcac
     2881 ggcggaatta taggaactgg atgattttga agttacggtt acgcagatta ttttttcgca
     2941 gtcatccccg acaccaagga acgccttcat aaattcatca gggctgggag cggatgtttt
     3001 cggtattacc gacgggtttt cccgcagtaa cctgaaaaag gtgttggtat caatgtccac
     3061 cccgtcggta aattgccgat tgccaaaaga cacggtaagg gggactatgg aaatatgcat
     3121 ggccgcggca tattcccgtg ggaaatcaca gcttgaatct gcgaaaactt ctatttttgc
     3181 cacagagggc acctgccttc caattgcacc aaaataagct caatgataat atagtattta
     3241 caatcatttg gcagatataa aagccccttg cagaataacg gagatttcca gtcggccacg
     3301 aaatgaaagc ttgtaaaaaa taaaatttca ttccatccga taagcgtagt ccacagttga
     3361 aatacattca aggatggaat ataataaaaa tgtctaacgt cgtttatcaa aaagaaactc
     3421 ctgctgtcat atggctacaa cgacggccgc ggcctttacc ccggatatgt cgaaaccggt
     3481 agaaataaag gaaatggaaa aggtacaggc catggatttg aaaaggacat cccggatatt
     3541 atttaatttt tctcttgtca ctgttttttt actgatcttc tgtttcctta tcctgcccga
     3601 ttccgccgaa ggcaatggta cccgggcggc gagcgcggaa tcttcttcgg gtaaagagca
     3661 gtcgattctg gaacagagca gagccgttac ctcttggggc cggatcgttt atggcaaaag
     3721 cggccttggg gcgaatttgg tctgttatca gatttgtccg gaaggaccaa taaaaaggaa
     3781 tatcttaatg acatttgaag ttcatggata tgaagacctt tttccggggg atggaaaggc
     3841 cttggtgaac atgggcaatt ccgtggccga ttattttatc aaaaatcggg ggcagcttca
     3901 aaattgcagg ctgtttcttg tggcctcggc aaatcccgag ggactgtctc atggaaaaag
     3961 caaggatggt atcggacgat gccaggcgtc cctcggggtg aatatcaacc gggattttga
     4021 ttttaatttt atcgtattta atgaaagccg tgaccatacg ctgagcgcgc cgttcgccgc
     4081 gcctgaaagc cgggctttgc gaaatctggt caatgctgtc agaccggata ttgtcattga
     4141 ctgccacgga tgggaaaatc actttatcgg cgaccactgg ctcgccggct tttttcgtga
     4201 cagcctcgga atcagccgga tcaactcgtt tgatcaaagc cagcacggct atttcagcgc
     4261 gtgggcaggt tcgaaaggga ttcagtccat gctgatcgaa tatccgacaa atgccgcgcg
     4321 taacagcggg ctatacgccg gcaaaacaat caccgggatt cggcgtctgg tgggcagtct
     4381 gtgaaagttt attttttcgc aatgattttt gatttctgcc aatggccgga gcgaatataa
     4441 atcagggcga tgataatgga gatcacggat gccgccggtg ctgccacgcc aacgccgaac
     4501 aaaccgtcgc gcagcacttt acttaaaaga aaagcgagtg gcacccgaac gaaaaatgag
     4561 gaaaccagcc cattgatcag cgagaaagtt gtgtgaccgc agccgtttac aaaaccgtcc
     4621 atacagaaaa caaaagacac catgatgaaa tcatagctga atgcacgcag ataaacaacg
     4681 ccgtccgcta tgacagccgc atccgcccga aacagatgca acaccgtttc cggaataatt
     4741 tgaacccata aaaagaatac aagggcgaaa gcaacggaaa acaaaatccc gacacgcatc
     4801 gtttttcgcg cccggtcatg ataaccggcg cccatgtttt gtgctgtcat cgatgcaatc
     4861 gctccggata acgcgctggc aggcagcatg gcaaatgtga tgaatttgtc ggaaataccg
     4921 accgccgctg aagccacaac gccaaagctg ttcacgatag ccgtgatgaa aagaaaagag
     4981 aaattgatca tcgtttcctg cgcgcagact ggaattccga gttttaaaag cgcggctgct
     5041 ttgtcttttt gaatccgaaa acttttcagt ttaaaatcaa agatgaaatc attctttttc
     5101 aggtagagaa ttgataaaac catgcttgcc gcctgcgcgg ttacggtgga tatggcggcg
     5161 cctgcaggac caagcctgaa aaagcctacg aatagaaaat cgagcagtat atttgtgaca
     5221 caggcgatag caataaaaat cagcggctgt ttggaattgc caaaccccct catgatcgcg
     5281 ctgatggcgt tatatccgac aataaatacg gtgccggcgg acgatatgta gacaaatgcc
     5341 tttgcctgag cgtatgcttc cggaggcgta ttcagacagt tcagaatcgg aatggtgaaa
     5401 gcgcccatca gcgcggtcag aacaacagcc agaatgaaaa agatcgtcag cattgtgccg
     5461 agcagttctt ttaaatcctg cttttgcatg gcgccaagat attgggcgat taaaattgtg
     5521 ccgcccaggg taatgccggt gaccagactg atcataatct gcataacctg actgccgatc
     5581 gaaatagccg cgatcccgcc tgcatttgaa aaccatccga cgatggccgt atcaatcgca
     5641 ccgtacatcg cctgcaagaa atttgcggca aaaaacggcg cggcgaatac ggctaatttt
     5701 tttgttacac tgccttcggt cagatttact tcaatgttgc tcaccatttc acgtccaatc
     5761 tgtctcaaat agtaaaaaag cggataaaaa attaacaaat gttaatctct tatccggcgt
     5821 atactcaaac gatacatcct ccgagcagat tcagtataac atataattat tttccataca
     5881 acttgtcctg catttgaagt ctgagtgaga agcgcggcat ccttgagagg ggatttttgt
     5941 gatgcggcgg tgtattttcc atgaaaccgc caagctaaat tagcataaat tgtaaattga
     6001 agcgggttat gttaaaatat aagaagtagg gacacggtaa cgtttgaacg gctataacag
     6061 aatgcacgaa caggtaagaa ggagtgatgc aagtgggtcc tgttgttaca ataaaaggcg
     6121 gggattatgt gttcgagtcg gattataaaa accgacccga gatccgccgc agttttaatt
     6181 gcctgaccga aaagactttt gaattcagtt tggagcaatg gtatcaggat gggtactggc
     6241 atgaaaacta cataccttac tccttgctga aaaacggcgc ggttgtgtcc aatgtctcgg
     6301 tcaatctcat ggattttcag ctttccggta agaaaaagcg ctatgtccag attggaaccg
     6361 ttatgaccga ttcggaattt cgggagcggg gtctgggccg gtatcttatg gaaaaagtgc
     6421 tggaggaatg gatagaacgc tgtgaccaga tttatctgtt ttccaatgac agcgctttgg
     6481 ccttctaccc gaagtttggg tttgaaaagg ccgatcagta tcaatgctcc agaaaagtat
     6541 accggaaagg ctcgtccatt tcctacagaa aactggagat gtcctcaaaa gaggacagaa
     6601 gattgtttga gaaggccgta tcgcagtcaa aaccgtccgc ccggctcgcg ctgctcaacc
     6661 cgctctccat tgtcgggtat tactgcggtt cggtgatgag agaccacata ctttatcttg
     6721 atgctctgga aacggccgta atcgcgggtg taaaaggggc aaccatctat ttgcacgatc
     6781 tgttttcccc acatggcatt tcggccgaac gggtgctgga agcgctgtat acccggaata
     6841 cacgcaaggt tgttctgggc tttacaccga tcgacaaacg gctgtacgat gaaaatcttc
     6901 tccaggaaaa agacaatacc ctttttgttt tgagcggaaa agagaacccg ttcaaaacgc
     6961 accggctgat gtttccggtt ctttcccggg cataaaaatc aaagttttat aagtgcggga
     7021 tgatgagaaa accgtgaaat cgctgtaaag tccagccagt ttatgatata ctggagcggt
     7081 tgtgattcgg ccggaaaaac tgcccgggct gagcgtaggc ccgcgcggcg atgattcttc
     7141 cggcgaaaat tgcgcaaacc gttttgcg
//
LOCUS       DATALY010000337         9802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_248947,
            whole genome shotgun sequence.
ACCESSION   DATALY010000337 DATALY010000000
VERSION     DATALY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248947"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1499)
                     /locus_tag="VHR42_09195"
     CDS             complement(180..1499)
                     /locus_tag="VHR42_09195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX3027381.1"
                     /translation="MRKTVIFLLFFCLTFTGIGTFQAEAAWNPTVPVTCNAVYLVNSD
                     TGTVVYEKNAHEKIYPASITKIMTALVTVKQLKDNLSKTVTVEQEDIDALSGTSSSTS
                     GLSVGENLTVEQLLYCMMLPSGNDAAVVLARVVGGSEGSFVRMMNDEAKSIGATNTHY
                     VNPHGLHDPSQYTTAYDTYLISREAMKDDKLAKIVDTTTYNMPKTNKNDARILTNRNG
                     LINPVSSYYYKYVRGIKTGTTSQAGACLASYAEKDGYTYYCIAMGGTENNGANSAFST
                     SKALYKWVFGNFQVSALLSQNDPQAEVKVELAWNIDSVLLVPDSQLNALVPDTFSQKD
                     VTVKPVNKPASVKAPLKKGQKVCRADVFLSGKKMGTVNLVSSKDIALSRPLYFVYIVG
                     VFFKSIWFKVISVLLILLFIGYLALSFSYNRRKKTLNRVKKKYKLPK"
     gene            1663..2949
                     /locus_tag="VHR42_09200"
     CDS             1663..2949
                     /locus_tag="VHR42_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX3027382.1"
                     /translation="MSDNQFKFDTLMIHAGQQPDSDTLSMSLPIYQTTAYAFRDVEHA
                     KNLFELKEGGNIYTRLQNPTNDVLEKRVAALDGGVGGLTVSSGHAAIFMTIANLCSAG
                     DEIVSSICIYGGAVNMMGISLDKLGIKVKFVNPDDLNAWEDAITDKTKACFVEGVGNP
                     NANVADIENIAKIAHSHGIPLIVDSTFTTPYLQRPIEWGADIVLHSATKFLGGHGTSM
                     SGVVVDSGNFEWLGNPRFPDFNQPDPGYHGIVYAKDFGKAAFITKLRTHILRDYGSCA
                     SPFNSFLILQGIETLSLRMRKHCDNALAVARYLNENPQVEFVHYPMLENNSYHSLAMK
                     YLPLGCGSVFTFGLKGGRERGARFINNLKLLSHVANVGDVRSLVIHPATTTHSQLSDE
                     QLEASGITPSTVRLSIGLEDASDIIADIDRAIEISK"
     gene            complement(3027..4040)
                     /locus_tag="VHR42_09205"
     CDS             complement(3027..4040)
                     /locus_tag="VHR42_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3027383.1"
                     /translation="MNGFFTLLGIENIKLWKRLSTKIMLIIMAVIIVSGVGLLKYYDY
                     RYAKNQSKSQSTVSDNWKAQTSAELVGLKSQLKAYESPSAGKMQKAFIGGTKIEIAKD
                     QYQLDHNVKPLESTNIWKKMMSFESDVNYGMLFALFLVIACSASVAGEFSEGTMKTMV
                     SRPFKRSEIMAAKLVSTILYGLIMLAASFLLTFLAIGIAFGFGGLSSGHLLWTGSKVI
                     LLPGFLKTAVIFGADFLQVLVYVVLAFAISAIFRSRSISTGFSLILLVLGTSLSQVAA
                     VYFDWGKYILFSLTNYNSYLGEGSSNFIIYGTSLPFALTVSAVYVAVMLFAGFFVFQK
                     RDI"
     gene            complement(4041..4970)
                     /locus_tag="VHR42_09210"
     CDS             complement(4041..4970)
                     /locus_tag="VHR42_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027384.1"
                     /translation="MSEIQPVLKLEKVKKVIGKRTIVSNISLELMPGEVFGFLGPNGA
                     GKTTTIKMVVGLSSITDGNISIGGHSVRHEYKAAMDNVGCIVENPEMYKYMSGMDNLK
                     IFAKMYKNVDGRRIAEVVKMVDLEHAIRDKVKTYSLGMKQRLGIAQALLHRPKLLILD
                     EPTNGLDPAGIRDMRELLRKLAAEEDLTVFVSSHILGEMQQMCDRVCIISNGSIITTK
                     TVEELVGMTNGGKSAALNVRTGDDERAAQQLKDAGASVGRTAEGIHVEVLREQIPEIA
                     KILVGAEIPVFAIENRESSSLEDVFMKLTGEDK"
     gene            complement(5019..5771)
                     /locus_tag="VHR42_09215"
     CDS             complement(5019..5771)
                     /locus_tag="VHR42_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027385.1"
                     /translation="MLLKLIKYEFKSSAVRFMAAFGAFTVIFGILLAFFRDHVVLLAA
                     VFGCGLVALSVITFLTLFHRYNTNLYGNEGYLMFTLPVSGKKILLAKLISALFWSLVL
                     GIATVIYIALYVCVMSPKFNLFQEILKLDIKPGTLIVCIVGIFVGLIVNILEIYFAIS
                     FSKLPVWRKFAVPAGFLVYIGANYLVSLPLAILSHVNQTSEIVIRFNSLQLDRQDLFF
                     SVNLYQLAFNILFSVVFFFATAFLLERKTCLK"
     gene            complement(5762..6463)
                     /locus_tag="VHR42_09220"
     CDS             complement(5762..6463)
                     /locus_tag="VHR42_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027386.1"
                     /translation="MGNLVEVEGLSKRYERKTALEGVSFSIPEGKIIGLLGPNGAGKT
                     TLIKILTGLISDYKGDVLIDGQEPGAHSKEIISFLPDKTYLSSWMKVSDTVDLFADFY
                     KDFDRKKANELLDRLNIDAGQKVTRLSKGTYEKVQLVLVMSREARLYILDEPIGGVDP
                     ASRDVILDTIISNYNEKSSVLLSTQLIQDVERIFDSIIILQEGRIIIHDEVDAVRAKH
                     GKSIDELFREVFKCC"
     gene            complement(6441..6833)
                     /locus_tag="VHR42_09225"
     CDS             complement(6441..6833)
                     /locus_tag="VHR42_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3027387.1"
                     /translation="MKNDFDSSLPIYIQIIGVIKKQIVSGERNPGQKVEPVREMAQKM
                     GVNPNTIQKAFAELEREGLMYSERTSGRYITGDTGLISKIREETAGKCIENFVSQMNR
                     SGFDRNDILRLVENYLRGVDNHGESCGG"
     gene            complement(7055..8719)
                     /locus_tag="VHR42_09230"
     CDS             complement(7055..8719)
                     /locus_tag="VHR42_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3027388.1"
                     /translation="MQGLNLKYVNETYDEKGALKTFSLNIPEDFNFAYDIVDEMARLE
                     PEKCAMVWCNPDGEERVFTYADMKKYSDKTANFLKSLGIGKGDMVMLILKRHYEFWFS
                     IVALHKLGAVTIPATALLTEKDLAYRFNTANIKAVICTADGEVSDYVDEAQKESPGLR
                     HKIIARGEKAGWLSFTDEVEAASDHFDRPTGEEAVNVHDIMLSYFTSGTSGMPKLVCH
                     DHTYPLAHIITAKHWHHVSPDGLHLTVAETGWGKAVWGKLYGQWIMEAAVFVYDFDKF
                     DAADLLTKMQKYKITTFCAPPTIYRFFIKNGIENYDLSSLTYATTAGEALNPEVFNKF
                     YQYTGIRLMEGFGQTETCLTIATLYGMEPRLGSMGKASPLYDLDLVDDDGKTVQSGEV
                     GEIAVKTNGNGQPGVFVGYYNDPERTRSVMHDGLYHTGDTAWRDEDGYFWYVGRTDDI
                     IKSSGYRIGPFEVESALMTHPAVLECAVTGAPDPVRGQVIKATIVLTKGYAPSDELAK
                     EIQNHVKHQTAPYKYPRVVEFVQDLPKTISGKIRRVEIRNKSAEKQ"
     gene            complement(8721..9317)
                     /locus_tag="VHR42_09235"
     CDS             complement(8721..9317)
                     /locus_tag="VHR42_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3027389.1"
                     /translation="MEMQILEIAERIRGLRQIMDISEQEMAQITGVNVEDYRDYENGK
                     NDFPFTFLLKSAERFGIDIVELMTGVDPKLSFYTVVRRGGGLPIERRKGFQYRHLAHL
                     MKNKIAEPFLVTAPYSEEEQNKPTTYSAHQGQEFDYILKGSLKVDFDGHVEILQAGDA
                     VYYDSGHRHGMIAAGGADCEFLAVVLKKQEEKTGKGDK"
     gene            complement(9372..>9802)
                     /locus_tag="VHR42_09240"
     CDS             complement(9372..>9802)
                     /locus_tag="VHR42_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX3027390.1"
                     /translation="DARFVNCDFTNADLSEAVIFRAEFLGCRIMGLDLNSASLKDVVF
                     EDCTGEYANFRFLRCERVRLSRCKFKGADFQQSRLMKTAVAECDFTSAQFSGTGLGGM
                     DLRSSIVEGLGVRPEDLSGAIVTPLQAVSFAKLLGLVIKD"
BASE COUNT         2459 a         2662 c         2196 g         2485 t
ORIGIN      
        1 atattgcgct ttcgaggccg ctttattttg tctatatcgt cggggtgttt tttaaatcga
       61 tttggtttaa agtgatatcc gcgctccggc gctgcccaaa tttgactccg atgaattcag
      121 cttataacaa aaacaaacgg acgccagata aggcgtccgt tttgctctca ttttaatttt
      181 tatttcggaa gcttatattt cttttttacc cggttaagcg tcttcttcct ccggttatag
      241 gaaaagctca aagcaagata cccgataaac agaaggatga ggagcacgga tatcacttta
      301 aaccaaatcg atttaaaaaa caccccgacg atatagacaa aataaagcgg cctcgaaagc
      361 gcaatatcct tgctggacac cagattcacc gtacccattt ttttaccgct caggaagacg
      421 tcggcgcggc agactttctg gccctttttc agcggcgctt tcaccgaggc cggtttattg
      481 accggcttaa ccgttacatc tttttggctg aaagtatccg gaacgagggc attaagctga
      541 ctgtcgggaa ccagcaaaac cgaatcgata ttccatgcaa gctccacttt cacctcagcc
      601 tgaggatcat tctggctcag cagggcgctg acctgaaaat taccaaacac ccatttataa
      661 agtgcctttg aggtggaaaa cgccgaattt gcaccgttgt tctccgttcc gcccattgcg
      721 atgcagtaat aggtgtatcc atctttttca gcataggagg caaggcaggc tccggcctga
      781 gaagtcgttc ccgttttgat gcctctgaca tatttataat aatatgacga tacgggatta
      841 atcagcccat tgcggttggt cagaattctc gcatcgtttt tattggtttt tggcatattg
      901 taagtggtcg tgtcaacgat tttcgccagc ttgtcgtcct tcatcgcctc gcgggaaatc
      961 aggtaggtat cgtaggcggt tgtatactgg ctggggtcgt gcaaaccgtg gggattcaca
     1021 tagtgggtat ttgtcgcgcc gatgcttttg gcctcgtcgt tcatcatccg gacgaagctc
     1081 ccctcgcttc cgcccaccac acgcgcaagc accacagcag cgtcgttgcc cgaaggcagc
     1141 atcatgcagt aaagcagctg ctcaaccgtc aggttttccc cgacactcag accggatgtg
     1201 ctgctggagg ttccactgag cgcatcaata tcctcctgct ccaccgttac cgtcttgctg
     1261 agattgtcct taagctgttt tacggtcaca agagccgtca tgattttggt aatggaagca
     1321 gggtaaatct tttcgtgcgc gtttttttcg tataccactg ttcccgtatc ggagttcacc
     1381 agatatacgg cgttgcaagt taccggaacc gtaggattcc aggctgcttc ggcctgaaac
     1441 gttcctattc cagtaaaagt cagacaaaaa aataataaaa atatcacagt ttttcgcatg
     1501 gacaatctct ccgaaaatgt tgtattatgt tattatataa acaacataat gtttattata
     1561 acagcaacat cgaccgcacg caaggccgca cgcaggcaat tagtcgaaaa attgcggcgg
     1621 cggttttcaa ccggccgtta aagaacaagg agtgtgaacc caatgtcgga caaccagttt
     1681 aaatttgaca ccctgatgat tcacgcgggt cagcagccgg acagcgatac gctttccatg
     1741 tcgcttccga tctaccagac gaccgcctac gcgttccggg atgtagaaca cgccaaaaac
     1801 ctgtttgaac tcaaagaggg cggaaatatt tacacccgtc tgcaaaatcc gaccaacgat
     1861 gttctcgaaa aacgagtcgc cgctctggac ggaggcgtcg gaggtcttac ggtctcctcc
     1921 ggccatgctg cgatttttat gactatcgcg aacctttgca gcgccggtga tgaaattgtg
     1981 tcgtcaatct gtatttatgg cggcgcggtc aatatgatgg gcatttcttt ggataaactt
     2041 ggtatcaagg ttaaatttgt caaccccgac gaccttaatg cgtgggagga cgcgatcacc
     2101 gacaagacaa aagcctgttt tgtcgagggc gtgggaaacc ccaacgccaa cgtcgccgat
     2161 attgagaaca ttgcaaaaat tgcgcacagc cacggcattc cgctgattgt ggattccacc
     2221 ttcaccacgc cttatctgca gaggcccatc gagtggggcg ccgatattgt cctccactcc
     2281 gccaccaagt ttctgggtgg ccacggcacc tccatgagcg gcgttgtcgt ggattccggc
     2341 aatttcgaat ggctcggcaa cccccggttc cccgatttca accagcccga tcccggctat
     2401 cacggcattg tctacgcaaa ggattttgga aaagcggcgt ttattaccaa gctgagaacc
     2461 cacatcctgc gtgattacgg ctcctgcgcc tcacccttca actctttcct gatccttcag
     2521 ggaatcgaga cgctctcatt aagaatgcgg aaacactgcg acaacgccct ggccgtcgcc
     2581 cgctacctga acgaaaatcc gcaggtcgag ttcgttcatt atcctatgct ggaaaacaat
     2641 tcataccata gccttgccat gaaatatctg cctctcggct gcggctcggt cttcaccttc
     2701 ggcctgaaag gcggacggga gcgcggcgcc cgctttatca acaatctgaa acttctctct
     2761 catgtcgcga atgtcggcga cgtccgctcg ctggtgatcc acccggcgac aaccacacac
     2821 agccagctca gcgacgaaca gctcgaggcc tccggcatca cgccttcaac cgtgcgcctt
     2881 tccatcggtc ttgaggacgc ttccgacatt attgccgata ttgaccgcgc tatagaaatc
     2941 agcaagtgat ttttaagaca atccaaaagc agcgggcttc gcaaaagcga agcccgctgc
     3001 tttttattga aataatgatg ctttactcag atatcccttt tctgaaagac gaaaaatcct
     3061 gcaaacagca tcaccgcgac ataaaccgcc gaaaccgtca gggcaaacgg aagcgaagtg
     3121 ccatagataa tgaaattgct gcttccttct ccgagatagc tgttgtaatt ggttaaggag
     3181 aacaaaatgt attttcccca gtcgaaatac actgccgcaa cctgcgagag gctagttccc
     3241 aaaacaagta agatcagcga aaagccggtg gatatggagc gcgaacggaa gattgcggag
     3301 atggcaaacg caagaaccac ataaaccaga acctgcagaa aatcggcccc gaaaatcaca
     3361 gccgttttga ggaatccggg aagcagaatc actttactgc ccgtccagag caggtgaccg
     3421 ctggacagcc ccccgaaacc gaaggcaatc ccgatcgcga ggaaagtaag aagaaaggac
     3481 gcggccagca taatcaagcc gtacagaatt gtgctgacaa gcttcgccgc catgatttcg
     3541 gaacgtttga acggccgcga aaccatcgtc ttcatcgttc cctcgctgaa ttctccggcg
     3601 accgaagccg aacaggcaat caccagaaac agcgcaaaca gcatgccgta gttaacatcc
     3661 gattcaaagc tcatcatttt cttccagata tttgtgctct caaggggctt tacattgtga
     3721 tcaagctgat actgatcttt cgcgatctca atttttgttc ccccgataaa ggctttctgc
     3781 atttttccgg ccgacgggct ttcataggct ttcagctgag acttcagacc gacaagctca
     3841 gcgcttgtct gggctttcca attatccgaa accgtgcttt gagatttcga ctggtttttt
     3901 gcgtagcggt agtcgtaata tttcaacagc ccgacgcccg aaacaataat aaccgccatg
     3961 atgatcagca tgatttttgt cgacaggcgt ttccagagct taatgttctc aattcctaag
     4021 agcgtgaaaa aaccgttcat ttatttgtcc tccccagtga gtttcatgaa aacgtcctca
     4081 agcgacgacg actccctgtt ttcaatcgca aatacgggaa tttccgctcc aaccagtatt
     4141 tttgcgattt caggaatctg ctctctcaga acttccacat ggatcccctc ggccgtcctg
     4201 ccgaccgacg ctcccgcgtc cttaagctgc tgcgcggctc tttcatcatc gcctgtccga
     4261 acattcagag cggcactctt cccgccgttt gtcatgccga caagctcctc gactgttttg
     4321 gttgtgatga tactcccgtt gctgatgatg cagacccggt cgcacatctg ctgcatttct
     4381 ccaagaatat ggctggaaac aaaaaccgtg agatcttcct ccgccgccag tttccggaga
     4441 agctcgcgca tatcccggat tcccgccggg tcaagcccgt tagtcggctc gtcaagaatc
     4501 agtagcttcg gcctgtggag cagcgcctga gcaattccaa gccgctgttt catacccagc
     4561 gaataagtct tgaccttatc ccggatcgca tgctcaaggt cgaccatctt taccacttcc
     4621 gcgattcgtc tgccgtcgac atttttatac attttggcaa agattttaag attatccatt
     4681 ccggacatat acttatacat ttccggattc tccacaatac agccgacatt gtccatcgcc
     4741 gctttgtatt catgtcggac cgaatgcccg ccgatggaga tgttgccgtc ggttatggaa
     4801 gacagaccga ccaccatttt gatcgttgtg gtcttgccgg ctccgttggg gcccagaaac
     4861 ccgaacacct ctcccggcat cagttccaga gaaatattgg aaacgatagt gcgctttcca
     4921 atcacttttt taactttttc cagtttgagc actggctgta tttcactcat gatgactcct
     4981 caagatattg atagatttgc agcttttgcc gcatgaattt attttaagca ggtttttctc
     5041 tcaagcaaga aggccgttgc aaaaaagaaa accacgctga acagaatatt gaacgccagc
     5101 tgataaagat tcaccgagaa aaacaaatcc tgtctgtcca gctgcaagct gttaaaacgg
     5161 atcactattt cagacgtttg atttacatgg gacagaatgg ccaggggaag agacaccaga
     5221 taattggcgc cgatgtatac gagaaaacct gcgggaaccg cgaattttct ccagacagga
     5281 agcttggaga acgatatggc aaagtaaatt tcaaggatat tcacgatcag gcccacaaag
     5341 atgccaacga tgcatacaat cagggtgccg ggcttgatat caagctttag aatttcctga
     5401 aacagattga acttcggaga cataacgcaa acgtaaaggg caatatagat gaccgtggca
     5461 attccaagaa ccaaagacca aaacagagcc gaaatcagtt tcgcaagcag aatctttttg
     5521 ccgctcaccg gaagagtaaa catcagataa ccttcattgc cgtacaaatt ggtattgtag
     5581 cggtgaaaga gggttaaaaa ggtgatgacc gagagcgcga ccagaccgca gccaaatacc
     5641 gccgcaagca agacaacgtg gtcacggaaa aaagcgagaa gaattccgaa aatcacggtg
     5701 aaggctccaa aggccgccat gaaacgcacg gcgctggatt taaattcata tttgattaat
     5761 ttcagcagca tttaaatacc tcccggaaca gttcgtcgat tgatttgccg tgttttgccc
     5821 gaaccgcatc gacctcgtca tggatgatga tccggccttc ctgaagaata attatactgt
     5881 caaaaatcct ctccacatcc tgaatcagct gggtggaaag caaaaccgag cttttctcgt
     5941 tgtaattgga aatgatcgtg tcgagaatca cgtcccttga agccggatcg acgccgccga
     6001 tcggctcatc aagaatataa agccgcgctt cccttgacat caccagcaca agctgaacct
     6061 tttcgtaggt tcccttggaa agcctggtaa ccttttggcc cgcatcaatg ttgagccggt
     6121 cgagcagctc atttgccttt ttccggtcaa aatccttata aaaatcggca aacagatcaa
     6181 cggtgtcgga aaccttcatc cagctgctca gataggtttt atccggaaga aaagatatga
     6241 tttctttaga gtgagccccc ggctcctgcc cgtcaatcag gacgtcaccc ttgtaatcgc
     6301 tgatcagccc ggtcagtatc ttaatgagcg tggtcttacc cgctccgttc gggcccagca
     6361 agccgattat ttttccttcg ggaatcgaaa acgatacccc ttccagcgcg gttttgcgct
     6421 catacctctt ggaaagcccc tcaacctcca caagattccc catgattatc aacccccctc
     6481 aaatagtttt ccacaagtct caatatatcg ttccggtcaa agcccgaacg gttcatctgg
     6541 ctgacaaaat tctcgatgca ttttccggcc gtttcctccc ggattttact gatcagcccc
     6601 gtgtcgcccg taatataccg ccccgatgtc ctctccgaat acatcaaacc ttcacgctcc
     6661 agttccgcaa acgctttttg aatggtgttt ggattcacgc ccattttttg cgccatctcc
     6721 ctcacaggct caactttttg ccccgggttt ctttctcctg atacaatctg ctttttaatc
     6781 acgccaataa tctgtatata aatcggcagg gaagaatcga aatcattttt caacagcctc
     6841 acccctttgc gattgtatta ttgtattaat aacttaatac aataatacaa tctttcttcc
     6901 tgtctgtcaa ggacttttcg caagttttct gcaacaaaaa ggcgaaggat gaccgctttc
     6961 gcggaaatcc ttcgctgcag cacccgttcc aatggccggg cagattttcg gttgtttgga
     7021 tgcttctccc gatcaagcgg taaaagcgtg tatcctactg cttttcagcg cttttattcc
     7081 tgatttcaac cctgcggatt tttccgctga tcgtcttggg aaggtcctgg acaaactcaa
     7141 cgactctcgg gtatttatag ggcgcggtct ggtgctttac atggttctgt atctcttttg
     7201 caagctcgtc ggacggcgca taacccttgg tgagcacaat ggtcgccttg atcacctggc
     7261 cgcgcacggg atcgggcgcg ccggtcaccg cgcattccaa cacagcggga tgcgtcatga
     7321 gcgcgctttc cacctcaaac ggcccgatac ggtaaccgga ggatttgatg atgtcatcgg
     7381 tacgtccgac ataccaaaaa tagccgtcct catcccgcca ggcagtatcg cccgtgtgat
     7441 agagaccgtc atgcatgacg gatctggtgc gctccgggtc gttgtaatag cctacgaaaa
     7501 ccccgggctg cccattgccg tttgttttta cggcaatttc gcctacttcg ccggactgta
     7561 cggtttttcc atcgtcgtcc acaagatcaa ggtcatagag cggcgacgcc ttgcccatgg
     7621 aacccagtct tggctccata ccataaaggg tcgcgatggt gagacacgtc tcggtctggc
     7681 caaagccctc catgagtctg atgccggtat actgataaaa cttattaaaa acctccggat
     7741 tgagcgcctc acctgctgtt gtcgcgtaag tgagcgacga caggtcatag ttctcgatac
     7801 cgtttttaat aaaaaagcgg tagatcgtcg gcggcgcaca gaaagtcgtg atcttgtact
     7861 tctgcatttt tgtcagcaga tcggcagcgt cgaatttgtc aaaatcatag acaaaaaccg
     7921 cagcctccat gatccactgg ccatagagct ttccccacac agccttaccc catccggtct
     7981 cggccaccgt cagatgcagc ccgtcgggac tgacatgatg ccagtgcttg gctgtgatga
     8041 tgtgcgcaag cgggtaggta tggtcgtggc agaccagctt cggcatcccg gaggtacccg
     8101 aggtgaaata gctaagcatg atgtcatgca cattcacggc ttcttcaccc gtcggtctgt
     8161 caaaatgatc cgaagccgct tcgacttcgt ctgtgaagct cagccatccc gctttttcac
     8221 ctctggcgat gatcttatgc ctgagccccg gggattcctt ctgcgcctca tcaacataat
     8281 cggaaacctc gccgtcggca gtacatataa cagccttgat gttggccgta ttaaagcggt
     8341 atgcaagatc cttctccgta agcagcgccg tagccggtat cgtcaccgca cccagcttgt
     8401 gcagcgcgac aatggagaac cagaattcat aatgccgctt caaaatcagc attaccatat
     8461 cgcccttgcc gatgccgaga ctttttaaaa agttcgccgt tttgtcgctg tatttcttca
     8521 tgtcggcata ggtaaatacg cgctcctcac catcgggatt acaccagacc atggcgcatt
     8581 tttccggctc aagccttgcc atttcatcga caatgtcata cgcaaaattg aaatcttcgg
     8641 gaatattgag cgaaaaggtt tttaacgcac ccttctcgtc ataggtctca ttaacatact
     8701 ttaaattgag tccctgcatt ttatttatct cccttaccgg ttttctcttc ctgctttttt
     8761 aaaacgaccg caagaaattc acaatccgct ccgccagccg caatcatacc gtggcggtgg
     8821 ccggaatcat aatagacggc gtcccccgcc tggagaatct caacatgtcc gtcaaaatcc
     8881 actttaagac tgcccttgag aatataatca aattcctgac cctgatgggc ggaataagtg
     8941 gtcggcttgt tttgctcttc ctcactgtag ggggccgtaa ccagaaacgg ctccgcaatc
     9001 ttgtttttca tcagatgggc gagatgccgg tactgaaagc ctttcctgcg ctcgatcggc
     9061 aggccccctc cgcgcctgac aaccgtataa aacgaaagct tcgggtctac tcctgtcata
     9121 agctccacaa tgtcgattcc gaaccgttcg gcgctcttaa gcaggaaagt aaacggaaaa
     9181 tcgtttttac cgttctcata atcacgataa tcttccacgt tgacgcctgt aatctgcgcc
     9241 atttcctgct cgctgatatc cataatctgc cgaagtcccc tgatacgctc cgcgatctct
     9301 aatatttgca tttccatttg atcagcctcc tgctattcaa tttattgttt tcgattttat
     9361 aggaataaac cttagtcttt tatgacaagc cccagcagtt ttgcaaaaga cacagcctgc
     9421 aaaggcgtaa cgatcgctcc cgaaagatcc tccggcctga ctcccagacc ttcgacaatg
     9481 ctgcttcgca gatccatccc cccgaggccg gtgcccgaaa actgggcgct ggtaaaatca
     9541 cattccgcca cggcggtttt cataagcctg ctctgctgaa aatcggcacc cttgaatttg
     9601 cacctgctca gacgaacgcg ctcacagcgc aaaaaacgaa aattagcata ttccccagtg
     9661 caatcctcaa aaacgacatc tttgagcgaa gcggaattaa gatccagccc catgatcctg
     9721 cagccgagga actcagcgcg gaagattacg gcttcgctca gatcggcgtt ggtaaaatca
     9781 cagttcacaa accgcgcatc ct
//
LOCUS       DATALY010000338         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_588241,
            whole genome shotgun sequence.
ACCESSION   DATALY010000338 DATALY010000000
VERSION     DATALY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588241"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..865
                     /locus_tag="VHR42_09245"
                     /pseudo
     CDS             1..865
                     /locus_tag="VHR42_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484096.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
     gene            complement(842..1126)
                     /locus_tag="VHR42_09250"
     CDS             complement(842..1126)
                     /locus_tag="VHR42_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027391.1"
                     /translation="MQQGNAQGQQLAITANLIAVQLSEFLSLDDQNIVGNLLALTGAS
                     LLSIAAVGAARSSNEDPSAASSTSSTGSNSSATTSASGSDTSGSDKQAGV"
     gene            1346..2119
                     /locus_tag="VHR42_09255"
     CDS             1346..2119
                     /locus_tag="VHR42_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX3027392.1"
                     /translation="MLNEFSREELLIGASGIKRLGSAQVAVFGIGGVGTYAAEALARA
                     GVGRIVLVDDDRVCLTNINRQLIATHQTIGKYKVDVMRDRILEINPAAQVVTHKVFYS
                     AETADELLNGRFDYILDAVDTVTAKIDLIVRAQEMGVPVISCMGAGNKTDPARFEVAD
                     IYATSVCPLAKVMRKELKARGIKSLKVVYSKEPPLKTIETAETSCKYHCVCPPGTARK
                     CTARRSIPGSLSFVPSVAGLIMTGEAVRDILAGPNKTNV"
     gene            complement(2404..>2709)
                     /locus_tag="VHR42_09260"
     CDS             complement(2404..>2709)
                     /locus_tag="VHR42_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX3027393.1"
                     /translation="QPFTYSRTVMLMDDFASVLSIADRVVLSEIMGSRETNTYHVYSK
                     DLAEKIDGCVWFPTFQEIADYILENASEGDLVITLGCGDIYKAAKLMLHKRASVTAV"
BASE COUNT          682 a          637 c          773 g          617 t
ORIGIN      
        1 gtcgacgaca atctcctttt cctcacgggc ggcggataca ggctggtcaa cgagatgcgg
       61 aaagaccgtt ccgctccgac cgcagcaaaa agtgaagaaa cagcaggaaa taccgcccgt
      121 cccgcctccc gtgaggaaaa ggagattgtc gtcgacgcat gcggcctgag ctgcccgggg
      181 ccgattatgt cggttcacaa agccatcgcg caggcggaga caggcgaagt gctgcttgtc
      241 cgtgctactg accccgcctt tgccggcgac atcagttcgt ggtgtgcgcg aacggggaac
      301 gagctgctca gttcggattt tgacggcgca agttttacgg tccgaatcaa gaagggggct
      361 tcctcctgcc ggctgaatca accggctcag ggcggaaacg gcaagagcat ggtggtgttt
      421 tcaggtgatc ttgacaaggc catcgcggcg tttatcatcg ccaacggagc tgcttccatg
      481 ggcagaacgg tgaatatgtt ctttaccttc tggggactga atatcctgcg taagcgggag
      541 aaatcccggg tcaaaaaaga ttttatctcc cgcatgatgg gaagaatgat gcccccgtgg
      601 ttccgaaaaa ctgggcttgt ccagaatgaa catgggtgga gccggcccga aaatgatccg
      661 gggaatcatg aaaaagaaga atattctctc gcttgaggat ctcattgcgc aggcgatcga
      721 aagcggcgtg aaaatcacgg cctgcagcat gtcgatggat gtgatgggca tcgcgcggga
      781 agagctgatt gacggcattg aaatcggagg ggttgcatcc ttcctggcaa gcgccgagga
      841 atcagacgcc agcctgttta tctgagccgg aggtgtctga tccgcttgcc gatgttgtgg
      901 cggagctgtt tgatccggtt gatgatgtgg aagaagccgc agaaggatcc tcatttgaag
      961 atcgcgcggc tccgacggcg gcgatgctca gcagggaagc gcctgtgagc gccaaaagat
     1021 ttcccacaat gttttgatca tcgagtgaaa gaaattccga cagctgaacg gcgatcagat
     1081 tcgcggtaat tgcaagctgt tgcccctgag cgtttccctg ctgcatggct gcaccccttc
     1141 cggaccctac tggtttatta tattttgccg aacctatgag ggtgaaaaat ttccataaat
     1201 cttcaaaatc aatcaattta tttcaaatgg cccttttcat tccggcggtt tcatattata
     1261 ataagattat tggataggtg ccgttggaag caaattgtgc cggctcggcg gcatgcggct
     1321 caaaagatct gcaaggggta aacggatgct gaatgaattt tcaagagaag aactcttgat
     1381 cggagcgtcg ggaattaaac ggcttggcag cgcgcaggtg gcggtgtttg gaatcggcgg
     1441 agtcggaact tacgcagcgg aagctctcgc ccgggccggg gttggcagga tcgtgctggt
     1501 ggatgatgac cgggtatgcc tgaccaatat caaccggcag ttgattgcta cccaccagac
     1561 gattgggaaa tataaagtgg atgtgatgag agacagaatt ctcgagatca atcccgccgc
     1621 acaagtcgta acccataagg tattctattc tgcggaaact gccgacgagc ttttaaacgg
     1681 ccggtttgac tatattcttg acgcggttga tacggttacg gccaaaattg atctgattgt
     1741 ccgggcacag gagatgggtg ttccagtcat cagctgcatg ggagcgggaa acaaaacgga
     1801 tcctgcgcgg tttgaggtag cggatatcta cgcaacctct gtttgtcctc ttgccaaggt
     1861 tatgagaaaa gagctgaagg caagaggaat caaatccctg aaagtggtct attccaagga
     1921 accgcctctc aaaacgatcg aaaccgcaga gacaagctgc aaatatcatt gtgtctgccc
     1981 gcctggaacg gccagaaagt gcaccgccag gaggtcgatt cccggaagcc tgtcgtttgt
     2041 tccatccgta gccgggctga ttatgaccgg tgaagcggtc agggatatcc ttgccgggcc
     2101 aaataaaacg aatgtttaga cgggagaaga cggagatgat ccattgacgg gaatgatttt
     2161 ttattcgata cccgttctgc tttacatagt ccttatcgcg cttgctgtca gccttgttaa
     2221 aaaaacaaac cttcgccggt tttataaccg gcgaaaatga gcaaatccgg gaaaaccggg
     2281 gcggattggc ggcgattcgg atcgagtgag ccgatcgaaa gagcaagacg gctttaaacg
     2341 aaacgagccc cattccactt caagcggaat ggggctttgt ttaaaagaaa aataccgggc
     2401 ggcttaaacg gccgtaacgg aagcccgttt gtgcagcatt aactttgcgg ccttgtaaat
     2461 gtctccgcat ccgagcgtaa tgaccaggtc tccctccgaa gcattttcaa gaatgtagtc
     2521 ggcaatttcc tgaaaagtcg ggaaccagac gcagccgtcg attttttccg caaggtcttt
     2581 ggaataaaca tgataggtgt tggtttccct tgagcccata atttcagaaa gcacgacacg
     2641 gtccgcaatg ctgagtaccg atgcgaaatc gtccatcagc attacggttc ttgaataggt
     2701 aaaaggctg
//
LOCUS       DATALY010000339         7885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_846938,
            whole genome shotgun sequence.
ACCESSION   DATALY010000339 DATALY010000000
VERSION     DATALY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846938"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHR42_09265"
     CDS             <1..223
                     /locus_tag="VHR42_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HEX3027394.1"
                     /translation="INNRNLKTFEVDLRTTEKLRRLIPPEIICVSESGIRDAGDVRRL
                     REIGVDAVLIGETLMRSDDVKKKMAELRG"
     gene            237..875
                     /locus_tag="VHR42_09270"
     CDS             237..875
                     /locus_tag="VHR42_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX3027395.1"
                     /translation="MTKIKICGLVSENDIEAANRLRPDYVGFVFAKSRRRLTLEKAAA
                     LNKLLDKSIGSVGIFVDERPDLVARMVRECGLAVVQLHGEEDGSYVESLRFLLPENCE
                     IWKAARIRSPEDIALAAATGADRLLLDAYSPYGRGGSGETFDWSLIGGYADVPFFLAG
                     GLTPENAGAAVRQVRPYGLDVSSGVETDGKKDDRKMERFVKAVRGASTQMER"
     gene            885..2072
                     /gene="trpB"
                     /locus_tag="VHR42_09275"
     CDS             885..2072
                     /gene="trpB"
                     /locus_tag="VHR42_09275"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX3027396.1"
                     /translation="MLQKGRYGVHGGQYVPETLMNALIELEKAYEFYKNDAEFNEELD
                     RLLKEYAGRPSLLYFAEKMTRDLGGAQIYLKREDLNHTGSHKINNVLGQALIAKKMGK
                     KRVIAETGAGQHGVATATAAALMGLECEIYMGREDTERQALNVFRMELLGAKVHPVDS
                     GTKTLKDAVSEAFREWTTRVDDTFYVLGSVMGPHPYPQIVRDFQSVIGREVRAEMLEK
                     HGRLPDMLIACVGGGSNAMGLFYDFIGDESVRLVGVEAAGEGVDTPRNAATITRGRIG
                     VFHGMKSYFLQDDDGQIAPVYSISAGLDYPGIGPEHAFLHDQGRAEYVFATDREAVEA
                     FLYLSRTEGIIPAVESAHAVAHAMRVAPTMSKDQIIVVNLSGRGDKDVASIARYLGVE
                     LSD"
     gene            2065..2868
                     /gene="trpA"
                     /locus_tag="VHR42_09280"
     CDS             2065..2868
                     /gene="trpA"
                     /locus_tag="VHR42_09280"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX3027397.1"
                     /translation="MTDRIAEMFQAKKTQGKKALITFVTAGDRCLEETERLVLEMENK
                     GADLVELGIPFSDPVAEGPVIQDANLRALKHKIRVNDVFDAVARLREKTQIPIVFLLY
                     FNCVLNDTPERFFKRCAQTGVDGVIIPDLPIEEREEAASVARSYGIKVIPLVAPTSQD
                     RIDKIAGEAEGFLYCVSSLGVTGMRSKFETDFESFFGSINKAAKTPTALGFGISSPEQ
                     AAKLKKYADGIIVGSAIVRLVGDSRSEDEAVEKVGGFVASLRAALDTDS"
     gene            3116..4165
                     /locus_tag="VHR42_09285"
     CDS             3116..4165
                     /locus_tag="VHR42_09285"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX3027398.1"
                     /translation="MNIFEEADQCISSRPKQKIGFFPTPIHKLEKLSKELGVNLYLKR
                     DDMTGVSLFGGNKIRKLEYLMGDAIRQGCDTVFTFGATQSNHAMQTVTACRRFGLTPI
                     LYLVAIVEPSEEDIRANLLLDRVMGAEIHIVPMNGKTEEEADDLSVELAEQHRKKLEA
                     EGHKCYMVPMGGANAVGSSGFVSGFVEMSRQMEKIGQHADYVFHGTGTGGTLAGLAAG
                     RALINSDTKIISINVSPKPIEHYEKIAELSTDTLAYLGSSKTVTVNDLHFDQNYYAPG
                     YEIPNQGAAEAIKLLARTEGVLLDPVYTGKGFAGMLDYIRTGKVPQGSTVVYWHTGGV
                     SALFAEKKIVGDICN"
     gene            4234..5598
                     /locus_tag="VHR42_09290"
     CDS             4234..5598
                     /locus_tag="VHR42_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX3027399.1"
                     /translation="MNLDLKCLAVDLPEDIKRLEYYGDFDGTLRVINKRLQKNLPIAL
                     RKRLELEKEIIKVLPTEYPYSLEAASEILNKELKGFKRSELLELKDQNVADWIYVNGE
                     IRFKNNFFANIVKTQPAFAARLKNPALAQDRKKNFELLNSVISDMKTKGNLTYRIHMR
                     TTLKIKKESERVGEPVKVHLPIPTVGFQAKNVKILSVLPEPAVIAPENYPQRTVCFEG
                     PLLSDQEFLTEYEYENHMVYVDPKPENVEDTQPDFYTQELLPHIMFTPYIRELTAEVL
                     GGEKNPLAKARKIYDFITTKITYSYVRPYLTITNIAEYAAVGLKGDCGVQALLFITMC
                     RCAGVPAKWQSGLYVTPLSVGNHDWAQFYLAPYGWLFADCSFGGSAYRIGEEEHWNFY
                     FGNLDPFRMPANSDFQCEFYPRKKFLRNDPYDNQSGEAEYENRGLSSLEYRTDHILVA
                     MKEI"
     gene            complement(5683..6276)
                     /locus_tag="VHR42_09295"
     CDS             complement(5683..6276)
                     /locus_tag="VHR42_09295"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX3027400.1"
                     /translation="MENNGSKFNTRFIVRTGLMAAVCFAATAINLKFSFTGATGSTMI
                     HLGSTAGFLAAMLFGGLSGGLAGGIGMGLFDLTGGYAFTAPWTLIIKFFAGYAVGKIA
                     WKNGKKGESQSTNLIACVTGGIINLVGYTIYTFLTTWIVFFSPSGTYFNNTLKAALLA
                     AGSQAVGSIATTILAIIIAIPFNDSIRKALKGSNLLK"
     gene            complement(6325..7188)
                     /locus_tag="VHR42_09300"
     CDS             complement(6325..7188)
                     /locus_tag="VHR42_09300"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEX3027401.1"
                     /translation="MTSRSPRVAAIHDLSGFGRCSLAVIMPVLAAMGVQPCPVPTAVL
                     STHTGGFSGPVIQDLTEFVSPCMDHWKRLKIEFDCIYSGFLASEKQAEQVEEFIHNWS
                     DALAVVDPVMGDDGRIYQTYSPALIAKIKELVKNADLITPNMTEACILLGQDFTNTAL
                     GRDNTRSMLVRLSEKGPGIVVITGAIVVPGRVSNVGYDRASNAFWRIDCDYVSANYPG
                     IGDIFSSVLVGGLLTGDSLAIAIARATRFVEISMRTAYGYNSEHRDGVMVEKSLPWLI
                     EKSTITGYEPL"
     gene            7574..7660
                     /locus_tag="VHR42_09305"
     tRNA            7574..7660
                     /locus_tag="VHR42_09305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7608..7610,aa:Ser,seq:tga)
     gene            7701..7790
                     /locus_tag="VHR42_09310"
     tRNA            7701..7790
                     /locus_tag="VHR42_09310"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7734..7736,aa:Ser,seq:gct)
BASE COUNT         2063 a         1768 c         2160 g         1894 t
ORIGIN      
        1 catcaacaac cggaatctca aaacctttga agtggatctt agaacaacgg aaaagctccg
       61 cagactcatt ccgccggaaa ttatctgcgt atcggaaagc ggaatacgcg acgccgggga
      121 tgtccgtcgg ctgagagaaa ttggcgttga cgccgtcctg atcggtgaaa cgctgatgcg
      181 ctcggacgat gttaaaaaga agatggccga gctgagagga tgaacgggag gctgaaatga
      241 cgaagataaa aatatgcggt ctggtcagcg aaaacgatat tgaggcggca aaccgcctcc
      301 ggcccgatta tgtggggttt gttttcgcta aaagccgccg acggctgact ttggagaaag
      361 cggctgcgtt aaacaaactg cttgataaat ccattggttc agtcggaatc tttgttgatg
      421 agcggccgga cctggttgcc cggatggtcc gggaatgcgg gcttgccgtg gttcagctcc
      481 acggggagga agatggaagc tatgtcgagt ctctcagatt cctcctgccg gaaaactgtg
      541 aaatttggaa agccgcccga atcaggtcac cggaggatat tgccctggcc gcggccaccg
      601 gagccgacag gcttttgctt gacgcttact ctccgtatgg ccggggagga agcggcgaaa
      661 cctttgactg gtctctgatc ggcggctatg ccgatgtccc tttttttctc gccggagggc
      721 tcactccgga aaacgcaggg gcggctgtca gacaggtcag accctatggc ctggatgtca
      781 gcagcggtgt ggagaccgac ggaaaaaaag atgataggaa aatggagcgg tttgtgaaag
      841 ccgtgcgcgg cgcttcaact caaatggaaa gataggtgag aacattgtta caaaaaggac
      901 gatacggcgt ccacggcggg cagtatgtgc ctgaaacgct gatgaacgcg ctcatagagc
      961 tggaaaaagc gtatgagttt tataaaaacg atgctgaatt taacgaagag cttgatcggc
     1021 ttctgaaaga atacgccggg agaccgtctc tgctatattt tgcagaaaaa atgacccggg
     1081 atctcggcgg agcgcagatt tatctgaagc gggaggattt gaaccatacc gggtcgcata
     1141 aaatcaacaa tgtgctgggt caggcgctga tcgccaaaaa gatgggcaaa aaaagagtga
     1201 ttgccgaaac gggagcaggg cagcatggcg tcgcgacggc gacagccgcc gctctgatgg
     1261 gccttgagtg cgagatctat atgggcaggg aagataccga acgtcaggcg ctgaacgtat
     1321 tccgtatgga acttctgggc gcgaaggttc atccggttga ttccggaacc aaaacgctga
     1381 aagacgccgt cagcgaagca ttccgcgaat ggacgacacg ggtggatgac accttttatg
     1441 tgctgggctc ggtgatgggg cctcatccct atccgcaaat cgttcgggat ttccagagtg
     1501 tgatcggccg cgaggtccgg gcggaaatgc ttgaaaaaca cggcaggctg cctgatatgc
     1561 tgattgcctg tgtgggcggc ggaagcaatg ccatgggtct gttttatgat tttatcggcg
     1621 atgaaagcgt tcggctggtg ggtgtggaag ccgccggaga aggcgtagac acgccccgga
     1681 acgccgcgac catcacaagg ggcagaatcg gcgtgtttca cggcatgaaa tcctattttt
     1741 tgcaggatga cgatggccag atcgctccgg tttattcgat ttccgcaggt ctggactacc
     1801 ccggaatcgg cccggaacat gccttcctgc atgatcaggg ccgcgccgag tacgtctttg
     1861 ccaccgaccg ggaagcggtg gaggcatttc tctatctttc caggaccgaa ggaattattc
     1921 cggctgtgga aagtgcccat gcggtggctc acgccatgag agtggcgccg acaatgtcaa
     1981 aggatcaaat tattgttgtc aacctgtcgg gccgcggcga taaggacgtg gcctcgattg
     2041 cgcgttattt gggggtggag ttaagtgact gacagaatag cggaaatgtt tcaggcgaaa
     2101 aaaacgcagg gcaaaaaagc cctgattaca tttgtaacgg ccggggatcg ttgtcttgag
     2161 gaaacggaac ggctagtgct cgagatggag aacaaaggag cggatctcgt cgaactggga
     2221 attccgtttt ccgacccggt tgctgagggc ccggtgattc aggacgccaa cctgcgcgcg
     2281 ctcaaacata agatccgtgt taacgacgtg tttgacgccg tggcaaggct cagagaaaaa
     2341 acgcagattc cgatagtgtt cctgctctat ttcaactgtg tgctcaacga taccccggag
     2401 aggtttttca aaagatgcgc tcagacaggc gtggacggcg tgattatccc agacctcccc
     2461 attgaggaaa gggaagaggc ggcatcggtt gcgagaagtt acggtatcaa agtaatcccg
     2521 ctcgtagcgc ctacgtcaca ggatcggatt gataagatcg ccggggaagc ggagggcttc
     2581 ttatattgtg tctcgtccct tggagttacc ggaatgcgga gcaaatttga aaccgacttt
     2641 gaaagcttct tcggcagcat caacaaagcc gccaagacgc cgaccgcatt gggattcgga
     2701 atctcgtcgc ccgagcaggc ggcgaagctg aaaaaatatg cggacgggat cattgtcggc
     2761 agcgcgattg tccgcctggt gggggactcg cgcagcgagg acgaggcggt ggaaaaggtc
     2821 ggcgggtttg tcgcgtcgct ccgggcagcg ctggatacgg attcttagag cttgcgctat
     2881 tgcttttacc cttgctgatt cctgaggata tgcttattcg ggtcccgtaa aaaacgggac
     2941 ccctttttta tgccgtttcg caattcgaag ggcaataggg ttaaatgatt ttgccgtaga
     3001 tgccttcggg gaaaacgccg ctatgaatct ggcccatgct ggaagcttat tctggacatt
     3061 ttgccgcccg gcatttaata taataatgaa taagtcatag gaggaatcgt ctgatatgaa
     3121 catttttgag gaagcggatc aatgtatttc ttcccggcca aaacaaaaga tcggtttttt
     3181 tcctacaccg attcacaaat tagagaaact ttcaaaagaa ctgggggtca atttatactt
     3241 aaaacgcgac gacatgacag gtgtcagcct tttcggcggg aacaaaatcc gtaaactgga
     3301 gtatttgatg ggggatgcga ttcgccaggg atgcgatact gttttcacct tcggcgccac
     3361 tcaatccaat catgccatgc agacggttac ggcctgccgc cggttcggat tgacccctat
     3421 cctttatttg gtcgcgattg tggaaccgtc ggaagaagat atccgtgcga atttattgct
     3481 tgacagagtg atgggcgctg aaatccatat tgttcccatg aatgggaaaa cagaggaaga
     3541 agcagatgat ttgtcggtgg aactggcgga gcagcatcgc aaaaaactgg aagcggaggg
     3601 gcataagtgc tacatggttc ccatgggcgg cgcgaatgcc gtcggctctt ccggatttgt
     3661 ttccggcttt gtggaaatga gccgccagat ggagaaaata gggcagcatg cggactacgt
     3721 ttttcacgga accggaacag ggggtacgct tgcgggtctg gccgccggca gggcactcat
     3781 caattccgat acaaaaatca tatcaatcaa tgtaagcccc aaaccgattg aacattatga
     3841 aaaaatcgcg gaactttcta ccgatacgct cgcttatctt ggaagcagca agacagtcac
     3901 cgtaaacgat cttcattttg atcagaatta ctacgctccc ggttacgaaa taccaaatca
     3961 aggcgctgcg gaggcgataa aactgttagc ccgcacggaa ggcgtactgc ttgacccggt
     4021 ttacaccgga aaaggttttg ccggcatgct ggactacata cgcaccggga aggtccctca
     4081 gggaagtacg gttgtttatt ggcataccgg cggagtgagc gcgcttttcg cggagaagaa
     4141 aattgtcgga gacatctgta actgaggtgt ttccgctctg acagctcttg cggcaacagc
     4201 agaatgatgc actgatgaaa atttggagga attatgaatc tggatttgaa atgtctggcg
     4261 gtagatttgc cggaagatat caagaggtta gaatattatg gagattttga cggaacgctt
     4321 cgcgtaatta acaaacgcct tcaaaagaat cttccgattg cgctcaggaa aaggctggaa
     4381 ttggagaagg aaatcataaa agttctcccg acagagtatc cgtatagtct tgaagccgcc
     4441 tccgaaatac tgaacaaaga gcttaaaggt ttcaaaagat cggaactgct ggaattgaag
     4501 gatcagaacg ttgccgactg gatctatgtg aatggggaaa tacgtttcaa aaataacttt
     4561 tttgcaaaca ttgtcaaaac ccagccggct tttgctgcgc gattaaagaa tcccgcgttg
     4621 gctcaagaca gaaagaaaaa ttttgagctt ctgaattcgg tgatttcaga tatgaagaca
     4681 aaaggaaact taacttatcg tatccatatg aggacaaccc tgaagattaa aaaagaatct
     4741 gaaagggtgg gtgaaccggt aaaagttcat ttaccgattc caacggtcgg atttcaggcg
     4801 aaaaacgtca aaatattaag cgtattaccg gaaccggcgg tgattgcgcc ggagaattat
     4861 ccacagcgaa cggtttgctt tgaagggccg ttattgtcgg atcaggaatt cttgacagaa
     4921 tatgagtatg aaaaccatat ggtttatgtg gaccccaaac cggagaatgt ggaggatacc
     4981 cagccggact tttacacgca ggaactgctt ccgcacatta tgtttacccc ctatatcagg
     5041 gaattaaccg cagaagtttt gggcggggaa aagaatcctc tggcaaaggc ccgtaaaatc
     5101 tatgatttca ttacaactaa aatcacctat tcttatgtga ggccgtatct taccattacg
     5161 aacattgccg aatacgcggc tgtcggtttg aagggagatt gcggggtgca ggcgctgctg
     5221 tttattacca tgtgccgctg cgcaggcgtt ccggcaaagt ggcagtccgg tttgtatgtt
     5281 actccgcttt ctgtaggaaa ccatgattgg gctcagttct atttagctcc atacggctgg
     5341 ctgtttgccg attgttcttt tggcggctct gcttatcgga tcggtgaaga agaacactgg
     5401 aatttctatt ttggaaatct ggatccattc cgtatgccgg caaattccga tttccagtgt
     5461 gagttttatc cgcgaaaaaa atttttaaga aacgaccctt atgataacca gagcggtgag
     5521 gcagaatatg aaaaccgtgg cctttccagt ctggaatata ggacagatca tattctggtt
     5581 gcaatgaaag agatttgaaa ttcaacgaaa attagtgcat aacgtatgca aaagaaacct
     5641 ccctgccggt aagcagggag gttttaagtt tgagcttttc tgttatttca gcagattgga
     5701 gcctttcagc gctttacgga tggagtcgtt gaacgggatt gcaatgatga tggccagaat
     5761 ggtcgtcgca attgagccga ccgcctggga accggctgcg aggagcgccg ctttcagggt
     5821 gttgttgaaa taagtcccgc ttggggagaa gaaaacgatc caggtggtca ggaaagtata
     5881 aatggtgtag ccgaccagat taatgattcc gccggtgacg caggcgatca gattggttga
     5941 ctgactttcg ccttttttgc cgtttttcca ggctattttg cctaccgcat agccggcgaa
     6001 aaacttgata atcagtgtcc agggcgcggt gaatgcataa cccccggtca aatcgaacag
     6061 gcccattccg atgccgcctg caagcccgcc ggaaagcccg ccaaacagca tggcggcgag
     6121 gaaccccgcg gtggagccga gatggatcat agtcgaaccc gtcgcaccgg taaagctgaa
     6181 tttcagatta atggctgttg cagcaaaaca gacagcagcc attagcccgg tacggacgat
     6241 aaatcttgtg ttgaattttg aaccgttgtt ttccataaaa atcccccagc attcttttga
     6301 ttattttaag cgcctttttt ttatttataa tggttcatac ccggtgatcg tgcttttttc
     6361 aatcagccag ggcagtgatt tttcgaccat gacgccgtct ctgtgttcgg agttgtagcc
     6421 ataagcggtt cgcatggaaa tttccacaaa tcgtgtcgcc cgggctatgg cgatcgcaag
     6481 gctgtcaccg gtgagaaggc ctccgacaag gacgctcgaa aaaatatcgc cgattcccgg
     6541 ataatttgcc gagacataat cgcagtcgat cctccaaaag gcattggacg cccggtcata
     6601 tcctacgttt gaaacccgtc ccggaacgac gatggcgccg gtgattacaa cgatacccgg
     6661 ccctttttcg gaaagccgga ccagcatgct tctcgtgtta tccctgccga gggcggtgtt
     6721 ggtgaaatcc tgcccgagca gaatgcacgc ttccgtcata ttcggagtaa tcagatccgc
     6781 gttttttacc agctctttga tcttggcgat cagcgccggg gagtaggtct gataaattct
     6841 tccgtcgtca cccattacgg ggtcgactac ggcaagggcg tccgaccagt tatggataaa
     6901 ctcttccacc tgttccgcct gcttttcact ggcaagaaaa ccgctgtata tgcagtcaaa
     6961 ttcaatttta agccttttcc agtgatccat gcagggtgaa acaaattcag tcagatcctg
     7021 aatcacaggg ccgctgaacc cgcctgtatg tgttgataag acagcggtgg gaacagggca
     7081 gggctgtacg cccatggcgg cgaggaccgg cataataacg gctaaggaac atcttccgaa
     7141 tcccgataga tcgtgaattg ccgctacacg cggcgaacgg cttgtcatga ggggcatgcc
     7201 tcctgtccgc ttcgtttcgg tattttgtcg caaatagttg ttattatcaa ttatatacgg
     7261 gaaatgagca gttgcaaaca cccaagaaag attgatttcg aaaaaatatt gtcacaataa
     7321 acaacagaag gctggtttga aatgataaaa cagggaattt ttattatgga acggatgcca
     7381 agttattaca gtgaaaagga ctgatgttaa tttgcatcag tccgttaata atatttgtaa
     7441 tgatattgtg caatatgcaa aagatattat tggttaaaaa tcaactgtcg gacttggaaa
     7501 taagaattca gaagtgcaat ttcgtattga caaaaaggtg attcttatgg taatataaac
     7561 tgcagcgcaa aacggagaga tgtccgagtg gtttatggag ctagtcttga aaactagtga
     7621 cccgaaaggg ccgggggttc gaatccctct ctctccgcca aagacgacat cttaataata
     7681 ttataacaat ttcaccatac ggagaagtac tcaagtggtg aagaggcgcc cctgctaagg
     7741 gtgtaggtcg ggtaaccggc gcgagggttc aaatccctcc ttctccgcca gactaagtct
     7801 ggacgcaatt cgcgtccgga ctttttcttt tactggagcc atctcactcg cgtttttatc
     7861 ttgctctttt ataacctaac tttcc
//
LOCUS       DATALY010000340         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_588463,
            whole genome shotgun sequence.
ACCESSION   DATALY010000340 DATALY010000000
VERSION     DATALY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588463"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="VHR42_09315"
     CDS             <1..110
                     /locus_tag="VHR42_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX3027402.1"
                     /translation="AQDAVKKAVDYITLSNDQDGVAHVIEKYIMQPSLA"
     gene            207..971
                     /locus_tag="VHR42_09320"
     CDS             207..971
                     /locus_tag="VHR42_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3027403.1"
                     /translation="MIREERLAKITEYVQNRHYASIEELVEMLGVSKATVRRDVLALS
                     KSNAVVLTRGGITCDLHDRNRELAYNEKRYANAAEKNRLAQTAGRMIRPDMTIIIDAG
                     TTTRSMVPFLREIKGLNLVTNDLMIAADVTDCEGISVTVTGGQLRRDFYTLRGYAAED
                     FVRHMRADIAFVGFDAVDIDSGCYITNADEVALKKCMIASADKVVALCDYTKFSNTAF
                     ISVCQLSDLDVIITNKELDMAEIEALRQMDVEVILA"
     gene            complement(1082..2755)
                     /locus_tag="VHR42_09325"
     CDS             complement(1082..2755)
                     /locus_tag="VHR42_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3027404.1"
                     /translation="MSAGNSPGPNGRIAIYARKSKVTEQGSSIEMQIEKCRRYAYYKY
                     DAKDSEIEIYQDEGLSGFYGDRPDFLRMLSDIDAEKIRAVVCYKYDRISRRTSDLSNL
                     VESLIEKKIDFAAASDEISSNTPSGRVLVFVLSAMAEFERNIIAERIGDNMFELAKTG
                     RWLGGNCPTGFRSQKEHFIRSGKKRVYNRLVPVESELDLVKKVFTIYLKERSLTKTAE
                     MINQWGFLTKNNAAFTRRSVRDLIINPVYSAADSSAYRYLKKNGASVYAEQNEFDGSH
                     GLMVYNKTEQTKNRESNSSFSRPKYVKKTVRRNITDWVVAIGEHDSTVTGHDWVKAQN
                     ILENNKKAVLSRGKSTALLSGLVFCPYCGSPLYVRAESGRLTSDGRQKFHYVCRKKYL
                     NGSSCDFRNVKGNELDRFVLETICSLPGADQKFFTDLRNHKKSLPLQTGKTDAEILKL
                     RKRQARAEKELRSQTENLRNAPNEIRESIYEDIQAILREQERIQSALGDLQLEQAKNC
                     DLHQAETVFSNFLALAESVGYQDKLELLHNIIDRVTADGDQVEIYFKTV"
     gene            2961..3173
                     /locus_tag="VHR42_09330"
     CDS             2961..3173
                     /locus_tag="VHR42_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027405.1"
                     /translation="MNFNAVIHLPENKNLELLYAAIAECRFDLLKNYLADSGLTADQV
                     GHILDNVLAKGSAVPGNQKGTRRVNY"
     gene            complement(3262..3465)
                     /locus_tag="VHR42_09335"
     CDS             complement(3262..3465)
                     /locus_tag="VHR42_09335"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX3027406.1"
                     /translation="MIKKYYSNAKDQDDLISIGTIGLIKAVSTFDGDKGTRLATYAAR
                     CIENATLTQMTRIILCSNSLAAF"
BASE COUNT          966 a          858 c          956 g         1019 t
ORIGIN      
        1 acgctcagga cgcggtcaaa aaagcggtcg attacataac gctttcgaac gaccaggatg
       61 gcgtcgctca tgtgatcgaa aaatatatca tgcagccgtc tctggcatag ctttcgcgca
      121 aactgaccag ctatgggtct gccgcgcata cggacagaat aagacgaatt gagaaaaggc
      181 catgacggaa tggcgaaggt gatagtttga ttagggaaga aagactggcc aaaattacgg
      241 aatatgtgca gaacagacac tacgcgagca tcgaggaact tgtggaaatg ctgggcgttt
      301 ccaaagcgac ggtccggcgc gatgttctcg cattgagcaa atccaacgcg gtggttctca
      361 ccaggggtgg aataacctgt gatttgcacg acagaaacag ggaactggca tataacgaaa
      421 agcgatacgc caacgcagct gaaaaaaacc ggttggctca aaccgccgga cggatgattc
      481 gccccgatat gaccattatc attgacgcgg gaacgacaac gcggtcaatg gtcccttttc
      541 tcagagagat caaagggctg aaccttgtga ccaacgattt gatgattgcg gccgatgtaa
      601 ccgattgtga aggaatcagc gtgacggtga ccggcggcca gctcagacgc gatttttata
      661 cgcttcgcgg ttatgccgca gaggattttg tccgccacat gagggcagat atcgcttttg
      721 tcggatttga cgcggtagat attgattccg gatgttatat caccaatgcc gacgaggtcg
      781 cgcttaaaaa atgtatgatt gcttcagcgg acaaagtcgt tgcgctgtgt gattacacca
      841 agttttccaa tacggcgttt atctcggttt gccagttgtc tgatcttgac gttatcatta
      901 ccaacaaaga attggatatg gcagaaatcg aggcgctcag gcagatggat gtcgaagtaa
      961 tcttagcgta aatgcgaacc gcggtgcgcc gatatgccgc cggcgcccgg cttgatactg
     1021 tgtgcgacgg ctcaaatgat cattaatgct ctttgatccg aatcaggatg gatcggggtg
     1081 atcagactgt tttaaaataa atttccacct gatcgccgtc agcggtgacc cggtctataa
     1141 tgttatgtaa aagttccagc ttgtcttgat agcctacgga ttcggccagc gcgagaaaat
     1201 ttgaaaaaac ggtttcggcc tgatgcaggt cgcaattttt agcctgttca agctgcaggt
     1261 cgcccaatgc ggactggatt ctctcctgct cccgtaaaat cgcctgtatg tcctcgtaga
     1321 tgctttcccg gatttcattc ggggcgtttc gcagattttc cgtctggctc cggagttctt
     1381 tttccgcccg ggcctgacgc tttcggagtt tcagaatctc ggcatctgtt ttacctgtct
     1441 gaagaggcag gcttttttta tggttacgga gatcggtgaa aaatttttga tccgcgccgg
     1501 gcagggaaca gatcgtttca agaacaaacc gatccagttc gttgcctttt acatttctga
     1561 aatcgcagga agaaccgttc aggtattttt tccggcaaac ataatggaat ttctgtcggc
     1621 cgtcgctggt aaggcggccg ctttccgccc ggacatacaa gggagaaccg cagtaggggc
     1681 aaaagaccag accggacaaa agagcggttg atttgcctcg ggacagtaca gcttttttgt
     1741 tgttttccag gatgttctgc gcttttaccc agtcgtggcc ggtaactgtg gagtcgtgtt
     1801 cgccgatggc gaccacccag tcagtgatgt ttctgcgaac cgtttttttc acatatttcg
     1861 gacgggaaaa agaggaattg ctttcccggt ttttggtctg ctccgtcttg ttgtaaacca
     1921 tcagcccgtg agaaccgtca aactcatttt gttccgcgta aacagaagcc ccgttttttt
     1981 taagataccg ataggcgctg ctgtcagcgg ccgaatagac agggtttatg atcaaatccc
     2041 gcacagagcg ccgggtaaag gcagcattgt ttttggtgag gaatccccac tgattgatca
     2101 tctccgccgt tttggtcagg gatctttcct tcaaatagat ggtgaaaacc ttttttacaa
     2161 gatccagctc ggattccacc ggaacaagcc gattgtatac ccgttttttt ccggatcgaa
     2221 tgaaatgttc tttctgggag cgaaaaccgg tcgggcagtt tcctccaagc catctgcctg
     2281 ttttggccag ttcaaacatg ttgtccccga ttcgttctgc gataatgttg cgctcaaatt
     2341 cagccatagc gctgagaaca aaaaccaaaa ccctgccgga aggcgtattg gaactgatct
     2401 catcgctggc ggcggcaaaa tcaatctttt tttcaattaa actctccacc agatttgaca
     2461 aatctgatgt tcttctgctg atccggtcat atttgtaaca aactacggcc cggatttttt
     2521 cggcgtcaat atcggaaagc attcgcagaa aatccggccg gtctccgtaa aaaccgctta
     2581 gcccttcgtc ctgatagatt tcgatttcgg agtcctttgc gtcatattta taataggcgt
     2641 agcgccggca tttttcaatc tgcatttcaa tgctgctgcc ctgttcggtc actttgcttt
     2701 tgcgtgcata aatggcaatc cgcccgtttg gcccgggact gtttcctgca gacataacca
     2761 gcctcctttg ggtcgctgcg tctattgatg cggcgtaccg gattttttta ttgtttgaag
     2821 tcatagtacg gcgcaggaaa tcatatcttt ttacggcgtt gttgggaagc gtcgtctttg
     2881 ttccggcttt tcccgtggtt tcgggctggg cgaacaggat tgacgggcaa cgttatcaac
     2941 tgcaacaggg ggcgctgtcc atgaatttca acgcggttat ccatctgccg gaaaataaga
     3001 atttggaact attatacgcg gcgatagcgg aatgtagatt tgatttactg aaaaattatc
     3061 ttgccgattc cgggctgaca gcggatcagg tcggccatat tcttgacaac gttcttgcga
     3121 agggatcggc tgttccgggc aatcagaagg gaacgcgtcg ggtcaattac tgataattgg
     3181 aacaaagagg aatgcaataa agatattcca ctcgtttgta attgcatccg atgccgccaa
     3241 agagagcggg aaaaacagaa atcaaaaagc ggccagactg ttcgaacaga ggatgattcg
     3301 tgtcatttgc gtcagggtag cattctctat gcatcttgcg gcataagtag caagccgggt
     3361 ccccttatcc ccgtcaaagg tggatacggc cttaattagt ccgatcgtgc cgatagagat
     3421 cagatcatcc tggtcttttg cattggagta gtatttcttt atcacaggct acgaaagcag
     3481 agattttaga gaggacggaa aacgcgccga accagatacg aaaaaagatg gactgactct
     3541 taaaaaaacg aaaacatgta ttgccataat ttgaatcaag cattaaaata ttaacaatat
     3601 gttttttcga accaaaggcc ttcgcttaga aaagaaaata ttggttaagg ccgttgagat
     3661 aatggactgg ccagagctgt ttcaaccatt ttcatatatg agagagtgat tgtatgttta
     3721 ccgtcccgat tatcagaaat aagctgattg tccctcaaaa tccagaatat tttgttggtt
     3781 caccaagaat taaggaatt
//
LOCUS       DATALY010000341         6592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_332233,
            whole genome shotgun sequence.
ACCESSION   DATALY010000341 DATALY010000000
VERSION     DATALY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332233"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VHR42_09340"
     CDS             <1..149
                     /locus_tag="VHR42_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX3027407.1"
                     /translation="GLLVGISAGAAVSAATQLAKSPENKGKKIVVLLPDTGERYLSTL
                     LFQE"
     gene            complement(352..1437)
                     /locus_tag="VHR42_09345"
     CDS             complement(352..1437)
                     /locus_tag="VHR42_09345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX3027408.1"
                     /translation="MKKKFVFLIIMIITVFVSGISLSVGAQSPGQPNITAESAMLVDL
                     KSGEILYGKALHQRMYPASLTKIMTGLIAVTRGHPDDVLTVSNNVIASVPRDTANAAL
                     SPGEKLTQEQALYTMFLASANDSAVAISEHIGGSVGEFVSMMNSYAGELGASDTHFSN
                     PDGLPDVKNYSSAYDLGLITRAAVQNPELMKYFGAVTYVMPPTNLRNQSVPYATLHKM
                     MKRTRYYYPGIIAGKTGWETMSGHNLVTVARRGGRTLICVTLKSANDASSYQDTQSLL
                     DYGFSLSPPQSGAQYLSTSVKGNKEVLASAFVKKGQELGKRLTPVLKRQRYPSGIMES
                     IFGILAIVCVGVVLYRLYASRQTDSLS"
     gene            complement(1650..2663)
                     /gene="galE"
                     /locus_tag="VHR42_09350"
     CDS             complement(1650..2663)
                     /gene="galE"
                     /locus_tag="VHR42_09350"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX3027409.1"
                     /translation="MNILVTGGAGYIGSHTCVELLNAGYGVVVIDNLCNSSEESLKRV
                     GKITGKALKFYQGDIQDRNLLDRIFEENPIDSVIHFAGLKAVGESVEKPLLYYQNNIS
                     GTITLCQSMAAHNVKKFVFSSSATVYGMPETVPVTEDFPLSVTNPYGRTKLMIEEILG
                     DIYTSDNSWSVALLRYFNPIGAHESGMIGEYPGGIPNNLMPYITQVAVGKLEYVRVFG
                     NDYDTKDGTGVRDYIHVVDLAKGHIRALEKIAKGPGLEAYNLGTGKGYSVLEVIQAFE
                     KASGIKIPYRILPRRAGDITATYANPDKALRELGWKAEKGIEEMCADSWRWQSGNPNG
                     YES"
     gene            complement(2933..4213)
                     /locus_tag="VHR42_09355"
     CDS             complement(2933..4213)
                     /locus_tag="VHR42_09355"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3027410.1"
                     /translation="MHLFRKASGVFKTTFRSLQYRNFRYFWFGQCISLIGTWMQRTAQ
                     VWLVYSITKSPLLVGILGVCQFTPTLLFTLFAGVLVDHFSKKHILIFTQAMFMLQAVA
                     MTALTYTGRIQYWHILVLSAVFGLTQTIDMPARQSFFIDMVGKKDVMNAVSLNSTIVN
                     VAKIVGPAVSGIIMVKYGPVFCFFVNACSYIAVIFGICMMRTPAVSTHRAAQRMGPEI
                     MEGVRYVRRNEVLMMNVILTAVVCTFAMNNDVIIPVFSKTVLGQGAGGYTGLMSAAGV
                     GAFIGAVLMAFISKNGLNKSILIIDGIATAAIQIATLFTRSYAVSLILIGIVGFINLT
                     FINTANSIFQVYSTDQYRGRVMSVYSFLVMGSTPIGNYFAGSVMESLGGDSGFVIGGI
                     VTLAIVIPYLIVKRKVAIEWLRAGRESYELSKAS"
     gene            4443..5057
                     /locus_tag="VHR42_09360"
     CDS             4443..5057
                     /locus_tag="VHR42_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEX3027411.1"
                     /translation="MENYENLNDIQKDVLREIGNIGGGNAATALSSILTDRVEMSVPK
                     LGVIGINEITEILGGPENEVVGILVPMSEDVRGMLLFILDKHFTHLLINALLNKNIDS
                     FENINEMDLSALKEMGNILSGSYISAISQLTNLNIMLSPPDIAIDMVGAILSYPAAMF
                     GMMGDKVLFIEEDFFSGIENIKSHLLIMPEMESLEIMLNRLGVV"
     gene            5057..5530
                     /locus_tag="VHR42_09365"
     CDS             5057..5530
                     /locus_tag="VHR42_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX3027412.1"
                     /translation="MSNLIVVGISDLKITRSPEILITYALGSCIGTCLYDSQLHIAGL
                     SHILLPESAISKSDHNVMKFADTAIEELVKAMEKIGSSRRRLTAKIAGGANMFASVGS
                     SIGERNVAAVKQELTRLNIRITGEDTGKNYGRTVEFHSENGDVIVKSFQHGNKIL"
     gene            5575..6078
                     /locus_tag="VHR42_09370"
     CDS             5575..6078
                     /locus_tag="VHR42_09370"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX3027413.1"
                     /translation="MPSSPNNPSTADPIHGRTPEESKIIMTELILPMQTNLLNNLLGG
                     QLMHFLDIAGALTCRRHSRGEVATVAVDSIEFKHPVRLGELITITSKMIWAGRTSMKV
                     QLEVAAENLKTGAFLITNTAYFTYVALCEDNTPCVIPPLLPQTEEEQRLFDLEEKKYR
                     ERKAKKQ"
     gene            complement(6134..>6592)
                     /locus_tag="VHR42_09375"
     CDS             complement(6134..>6592)
                     /locus_tag="VHR42_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX3027414.1"
                     /translation="PAGEWGELVITTLKKQAAPLVRYRTHDITRLIDGVCPCGRSYPM
                     IDRIMGRTDDMIKVKGVNIYPGQVDSLLKSCNGVSSEYQIHISHENGRDVMLLKVERQ
                     PGIEGGCVEREVMDNFKRQIGIRIGVESVEIGALPRSEKKSKRVFDYRAG"
BASE COUNT         1772 a         1660 c         1488 g         1672 t
ORIGIN      
        1 aaggtctcct ggtcggaata tccgccggag ccgctgtcag cgcggcaact cagctggcaa
       61 aaagtccgga aaacaagggt aaaaaaattg tcgttctgct gcccgacacc ggagaaagat
      121 atctttccac actacttttc caggaataag gcaaaacaaa attttcgatg gcttaaagta
      181 aagtaagcct cacggtattc gtgaggctta ctttatttat aaattttaaa acacaaaaag
      241 aaagccggcg tccgaaccga gacgacggcc cttgattcat ggagattcta cccatgacat
      301 ccgaaagccc cgtggacttc cgtccatggg gctgaaaaca tccggtatct atcaggatag
      361 gctgtcggtc tgacgcgatg cgtagagccg gtagagcact acccccacac aaacgatcgc
      421 gagtattcca aaaatgcttt ccataatacc gctcgggtag cgctgccgtt ttaaaacagg
      481 agtcaggcgt ttgccgagtt cttgtccctt ttttacgaat gcagaagcaa gaacctcctt
      541 gtttcctttc acggatgtcg acagatactg cgctccggat tgtggaggac tcagtgaaaa
      601 accataatcc agcagcgact gcgtatcctg atagctggaa gcgtcattgg cgcttttcag
      661 tgtaacgcaa atcagggttc tgccgccgcg gcgcgcgacg gtcaccaggt tatgacctga
      721 catggtctcc cagccggttt ttcccgctat aatgcccggg taataatagc gggtcctttt
      781 catcattttg tgaagtgtcg cataaggcac cgattgattc cgcaagttgg tgggaggcat
      841 gacgtaggtg accgctccga aatatttcat caattccgga ttttgtaccg ccgcgcgtgt
      901 gataagccca agatcgtagg cgctcgagta gtttttgaca tcgggaagcc cgtccggatt
      961 tgaaaaatga gtatcggacg cacccagctc cccggcgtaa gaattcatca ttgatacaaa
     1021 ttccccgaca cttccgccga tatgctccga aatcgccacg gcggaatcat tggcggaggc
     1081 gagaaacatg gtataaagag cctgttcctg tgtcagcttt tccccaggcg acagggccgc
     1141 attggccgtg tctctcggaa cgctcgcaat tacattattg ctgacggtaa gaacatcatc
     1201 cggatgtccc cgggtcaccg cgattaaacc ggtcatgatt ttggtaaggc tcgccggata
     1261 cattctttga tgcaacgcct taccgtacaa aatctcaccg ctttttaaat cgacaagcat
     1321 tgcggattcc gcggtgatat tcggttgacc cggcgactgc gcgccgacgg aaagactaat
     1381 gccggaaaca aacaccgtta tgatcattat gattaaaaat acaaactttt ttttcatagt
     1441 tccccattta tttgttcttt ttttactttc tttcagactg ttttcgataa aattttaaca
     1501 taaaaggaaa gtaatttcaa gtcgttttgt tcttaatttg taactaaata gtaactattt
     1561 ttaaaaacac aaaaagaaag ccggcgtccg aaccgagacg acggccctta atttcttgat
     1621 ttatgagatt ctacccgtga tatccgattt cagctttcat atccgtttgg attacccgac
     1681 tgccaacgcc aggaatccgc gcacatctct tcaattccct tttccgcttt ccagccaagt
     1741 tcgcggagcg ctttatccgg attggcatag gtagccgtaa tatccccggc acgtcttggc
     1801 aggattcgat agggaatttt gatcccgctg gccttttcaa acgcttggat gacttccaga
     1861 acgctgtatc ccttcccggt tcccagatta tacgcttcaa ggcccggtcc ttttgcgatc
     1921 ttttccagcg ccctgatatg accttttgca agatccacga catgaatata atcgcgaacg
     1981 ccggtgccgt ccttggtatc ataatcattt ccgaatactc tgacatactc cagttttccc
     2041 acagccacct gcgtaatata cggcatcagg ttattgggga ttcctcccgg gtattcaccg
     2101 atcattccgc tttcatgcgc tccaatcggg ttaaaatatc tcagaagggc aacgctccag
     2161 ctgttgtcgg aggtgtaaat atccccaaga atttcctcga tcatcaactt ggtgcggcca
     2221 tagggattgg tcacggacaa aggaaaatcc tctgtaaccg gaacagtttc cggcattcca
     2281 tagacggttg ccgaggaact gaaaacgaat tttttaacat tgtgcgccgc catcgactga
     2341 caaagcgtga tggttcccga aatattattc tgataataaa gaagcggttt ttcgaccgat
     2401 tcgcctaccg ctttcaggcc ggcgaaatga atgaccgaat caatcgggtt ctcttcaaaa
     2461 atccggtcga gaagattcct atcctgaatg tctccctgat aaaatttaag cgctttaccc
     2521 gttatcttac ccactctttt aagcgattct tcactgctgt tgcaaagatt atcaataacg
     2581 acaaccccgt aacccgcatt caataattcg acgcaggtat ggctgccgat atatccggcc
     2641 cctccggtta caagtatatt cattcgatca cacctacaat tttttatttt aaggagtatt
     2701 tatgagtgct attatattcc gaacggcaga ttctgtcaaa ttacaacaac gttttgaccg
     2761 gccccgcgcc ggcagaaata tcggaccctt tacgaaattt tattaatgaa accggcaaac
     2821 agatcatata gtgataacag cagaagaaat aaaagcaact gataagacag gggccttatc
     2881 atgtgattta tttcactgcg agggcgccct ttacgggcgt ccgttgccat tttcagctgg
     2941 cttttgagag ctcgtagctt tccctccccg ccctcagcca ttcaattgcc acttttcttt
     3001 ttacgatcag ataagggatg acaatggcaa gcgtcacgat tcctccgatt acaaatccgg
     3061 aatctccccc cagtgattcc atcaccgagc ctgcaaaata attcccgatc ggcgtggaac
     3121 ccataacaag aaaggaataa acgctcatga ctctgccgcg gtattgatcg gtcgaataaa
     3181 cctgaaatat agagtttgcg gtattgataa aggtcagatt gataaaaccg acgattccga
     3241 tcagaatcag gctgaccgcg taagaccggg tgaaaagcgt tgcgatctga atcgcggcgg
     3301 tagcgattcc gtcaatgatc agaatacttt tgttcagacc gtttttactg ataaaagcca
     3361 tcagcacggc gccgatgaaa gctcccacac cggccgccga catcagcccc gtatagccgc
     3421 cggccccctg cccaagcacg gttttcgaga aaaccggtat gatgacgtcg ttattcatgg
     3481 caaacgtgca gaccaccgcg gtcagaatga cattcatcat cagcacttcg ttgcgtctga
     3541 cataacggac gccctccatg atttccggcc ccatccgctg cgcggcgcga tgcgtggata
     3601 cggcgggcgt ccgcatcatg cagataccga aaatcaccgc gatgtagctg caggcgttaa
     3661 caaaaaagca aaataccggg ccgtacttta ccataattat gccggagacc gccgggccga
     3721 caattttggc aacgttgacg attgttgaat ttaaagaaac ggcattcatg acatcctttt
     3781 tacccaccat atcgataaaa aaagactgcc tcgccggcat gtcgatcgtc tgggtcaggc
     3841 caaaaacagc actcagaacc aaaatgtgcc aatattgaat tctgcccgtg taagtaaggg
     3901 cggtcatggc cacggcctga agcataaaca tcgcctgcgt aaaaatcaga atatgttttt
     3961 tggaaaaatg atctaccagt acgccggcaa acagggtgaa aagcagcgtc ggcgtaaact
     4021 ggcacacacc gagaattccc accagcagag gagatttcgt aatggagtaa acgagccaca
     4081 cctgcgcggt tctctgcatc caggtgccga tcaacgaaat acactggccg aaccaaaaat
     4141 accggaaatt tcgatattga agcgatcgga aggttgtttt aaaaactcct gaggctttac
     4201 ggaacaaatg catgaatgtc accttactta attgaatgta atttaattat actaccgctg
     4261 gcaaggcctg atttttgagg atgacgcaaa cttcgcccac ccttgatttt cagaaaatgc
     4321 cgactgtttc aaccctattg atttcttgaa cggtttgttt taaaatagaa atatgaaacg
     4381 gctaaaaatg gaagctcggc gcggttccag cgccacagac tgagcaaaaa ggagatctgc
     4441 tgttggaaaa ctatgaaaac ctcaatgata ttcaaaaaga cgttttaagg gaaatcggca
     4501 atataggcgg aggcaatgcg gcgactgccc tgtcatccat tctgaccgac cgggttgaaa
     4561 tgtcggttcc caaactcggc gtaatcggta tcaatgaaat taccgaaatt ctcggagggc
     4621 cggaaaatga agtagtcggc atcctggttc ccatgtcgga agatgtccgc ggaatgcttc
     4681 tctttatact ggacaagcat tttacccacc ttctgatcaa cgcgcttctc aacaaaaata
     4741 tcgacagctt tgaaaatatt aacgagatgg atctgtcggc gcttaaagaa atgggtaata
     4801 ttttatccgg ctcatacatc agcgcaatca gccagctcac caatttaaac attatgcttt
     4861 cgccccctga tatcgccatt gacatggtcg gcgcgattct cagctaccct gcggcgatgt
     4921 ttggaatgat gggtgacaag gttctcttta ttgaagaaga ttttttcagc ggaatagaga
     4981 atattaaaag tcacctgctt atcatgcctg aaatggaatc tctggaaata atgttgaatc
     5041 gtttaggtgt tgtttgatgt ctaatttaat cgtagtcggc atttctgatc ttaaaatcac
     5101 ccgttcgccc gaaatcctga ttacttacgc gcttggctcc tgcatcggga cctgccttta
     5161 tgacagtcag cttcacattg cagggctctc tcacattctg ttgccggaat ccgcaatcag
     5221 caaaagtgac cataatgtta tgaaatttgc agacacggcg attgaagaac tggtcaaagc
     5281 gatggaaaag atcggatcgt caagacggag gctcacggcc aaaatagccg gcggggcgaa
     5341 tatgttcgct tcggtcggaa gcagcatcgg agagcggaac gtcgcggctg taaaacagga
     5401 actgaccagg ctcaatatcc gcataaccgg cgaggatacc gggaaaaatt acggaagaac
     5461 ggtagaattt cattcggaaa acggagacgt aattgtcaaa tcatttcagc atggaaataa
     5521 aatactctaa aacaacttct atattatatc gtatgtaaca gaaggagaat ctatatgcct
     5581 tcctcaccca ataatccttc cactgccgat cccatccacg gcagaacacc ggaagagtct
     5641 aaaatcatta tgacggaact tattctcccg atgcagacca atcttttaaa caacctgctc
     5701 ggcggacagc tgatgcattt tcttgatatt gccggcgcgc tgacctgcag aagacactca
     5761 aggggcgaag tggctaccgt tgccgtcgac agtatcgaat tcaagcatcc cgtccggctg
     5821 ggtgagctaa ttacaatcac ttcaaaaatg atttgggccg gccgaacctc tatgaaagtc
     5881 caactggagg tcgcggctga aaatctgaaa accggagcgt ttctgatcac caataccgca
     5941 tattttactt atgttgccct gtgtgaagat aacacgccct gtgtgattcc tccccttctt
     6001 ccgcaaacgg aggaggaaca gcgtttgttt gatcttgagg agaaaaaata tcgggaacga
     6061 aaagcgaaaa agcaataaaa tatcggagcc aaaaccgtta tccacggctt tggctccggc
     6121 tttttcacag ccgtcatccg gcccggtaat caaaaaccct tttgctcttt ttttcacttc
     6181 tcggcagcgc gccgatttca accgattcga cgccgatacg gatgccgatc tggcgcttaa
     6241 aattgtccat gacctcccgc tccacacagc cgccttcgat tcccggctgc ctttccacct
     6301 tcagcagcat gacatccctg ccattttcgt ggctgatgtg aatctgatat tcactgctga
     6361 ccccattgca gcttttcaga aggctgtcga cctgaccggg ataaatgttg acgcctttga
     6421 ccttgatcat atcatcggtt cttcccataa tgcggtcgat catcgggtaa cttcttccac
     6481 aggggcagac gccgtcgatc aggcgggtga tgtcgtgggt gcggtagcga accagcggag
     6541 ccgcctgctt cttcagcgtg gtgattacca gctcgcccca ttcgccggcc gg
//
LOCUS       DATALY010000342         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_678354,
            whole genome shotgun sequence.
ACCESSION   DATALY010000342 DATALY010000000
VERSION     DATALY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678354"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VHR42_09380"
     CDS             complement(<1..230)
                     /locus_tag="VHR42_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA domain-containing protein"
                     /protein_id="HEX3027415.1"
                     /translation="MGKYLNEPDLYIIIAGVIMGTLARFFTLRVDYRQIPTYPSAYFN
                     NIVMGFIASALGAIAIPAVLTGDFVSITFLTVA"
     gene            321..2081
                     /locus_tag="VHR42_09385"
                     /pseudo
     CDS             321..2081
                     /locus_tag="VHR42_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
     gene            complement(2147..>2657)
                     /locus_tag="VHR42_09390"
     CDS             complement(2147..>2657)
                     /locus_tag="VHR42_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3027416.1"
                     /translation="FIMRDLNPLNKETGKGKPGPAWTVGAQAVEIEFDRKDDTYRFVR
                     AATVIDAGKVLNPKMVDGLIKGGMCMGLGLGSREYFNYDADGRVLDTSLRTYKVMHFG
                     ENPKYIVDFVETPQGDAPYGERGLAEHGIIGIPAALGNALSLAAEADLDELPLTPEAI
                     WRQKKGGKP"
BASE COUNT          687 a          683 c          675 g          612 t
ORIGIN      
        1 gctacagtca aaaatgttat agaaacaaaa tcaccggtta atacggctgg tattgcaatt
       61 gcacccagag cagaggcgat aaaccccatc acgatattgt taaaataggc gctcggatag
      121 gtgggtatct gccggtagtc aaccctgagg gtaaagaacc gtgccaaagt gcccatgata
      181 actccggcaa tgatgatata caaatccggc tcattcagat attttcccaa atagatcacc
      241 ctatgatatt atcttcacga attggaaata ttattaatgc caaattgagc tggtaattgc
      301 atgaaccggg aagtgaaaaa gtggctgaac aacagatcgg gaaaacagta atccgcaaag
      361 aggcatggga taaggttacg gggagcgcga aatatgtaaa cgacgagatt acggtgggca
      421 tcctgactgc acgcatcgca accagtacct tcgcccacgc gacgatcaaa aacatcgatc
      481 tttccgcagc atctgcgctc cccggggtca aggcgatttt aaaaggggac gatgacccgg
      541 ttttatatgg gccgctgatc gacgaccgtc ctccgctggc agttcatgaa gtgcggtatt
      601 acggagagcc tgtcgcattg gtgatcgcag acagcgatta tatcgccgaa aaagcagtta
      661 ctctgattaa aatagattac gagccccttc ccgcccttca atcgccgtca caggctattg
      721 agaagagcgc cgtccttctg catccaagat taggcgaata caaacacgcg gtggatgata
      781 tcgatccgga gccgggaaca aatatctgtg accggaaaaa gatccgcaaa ggcgatatcg
      841 acaaggggtt ttccgagtcg gatgtcacca tcgaagctca gttcaaactt cctcagtcag
      901 atcatatcgc gatggagcct cgtactgcca gagcgcggat tgctccggac ggaaccgttt
      961 acatcaaatc ttcttcccaa tcgccgtttg caataaaaaa acagatcagc cggattttta
     1021 acatcgagca ggggaaagtg gtagtcgaag ttccctttgt cggcggcggg tttggaggca
     1081 aagctccgat tcagcttgaa atattggcct ttatggccgc taaggctgtg gacgggaagg
     1141 aagttcgaat tgtcaattcg cgcgaaaatg acattgcttc atccccatgc cggatgggcc
     1201 ttgaagccgt cataaaactt ggcgctgcaa aggacggcac tctgaaagct gctcagatga
     1261 ccttccttgt ggacaccggt gcatactccg atatcggccc gaggctttcc aaatccatag
     1321 ctgtcgactg cacggggcca tacagaatcg acaacgtccg gtgcgattcc ctttgcgtat
     1381 atacgaatca tccgtacgcc acttcgttcc gtggattcgg tcacgcctgc tatacctttt
     1441 gcattgagcg cgcgctcgac aagctttctg aaaaactggg acttgaccct ttggagctgc
     1501 ggctgaaaaa tgcagtgcag ccgggcgaca ccaatccgac acaggtgaaa atgacttgga
     1561 gcaacctcgg gaaccttccg gcctgcatcg aaaagctgaa aacgatcatc aattgggatg
     1621 agggccggaa aattgaatta ggcaatgaca aggtcagggc aaaagggatc agttgtctat
     1681 ggaaaacctc cgattccccg actgatgcga tttcgggcgt aatcctgact tttaataagg
     1741 acggcagcat caatctcaac tgcgggtgcg tggaaatcgg gccggggatg aaaacgactc
     1801 cggctatcat tctggcggaa aaattaaaga tggatatcaa ccggattcac gtttcgatgg
     1861 atgtgaacac aaagtattgc cccgagcact ggaaaacagt ggccagcatg accacttata
     1921 tgatcgggcg cgctgccttg cgagccgccg aagatgctgc ccgccagctg aaaagcgccg
     1981 ccgctttggt gcttcggtgc acgccggagg atctggatat cgcggacgag cgggtttttt
     2041 tatcttacga cccttctgtt tctgttgaat ttaaagattt ggttcatggg tatcaatatg
     2101 acaacgggaa ttccgtcggg ccgataatag tcaaagtcga aaggaatcac ggcttgcctc
     2161 ccttcttttg gcgccagatt gcttccgggg ttagcggcag ctcgtccaaa tcggcttccg
     2221 ccgcaagcga caaggcattg ccaagtgccg cgggaatccc gatgatccca tgctcagcca
     2281 gtccgcgttc tccatacggg gcgtcgccct gcggcgtttc cacaaaatca acaatatatt
     2341 tcggattctc tccaaaatgc attaccttgt aggtgcgaag gcttgtgtcc aaaaccctgc
     2401 cgtccgcatc ataattgaaa tattcccggc tgcccagccc gaggcccatg cacataccgc
     2461 cttttatcag cccatccacc attttgggat taagcacctt tcctgcatca atgacggtgg
     2521 cagcgcgcac aaaacggtag gtatcgtctt tcctgtcaaa ctcaatttca actgcctgag
     2581 ccccgactgt ccacgcgggc ccgggctttc cttttcctgt ctccttgttc agcgggttca
     2641 ggtccctcat gatgaag
//
LOCUS       DATALY010000343         7456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_333004,
            whole genome shotgun sequence.
ACCESSION   DATALY010000343 DATALY010000000
VERSION     DATALY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333004"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1139
                     /locus_tag="VHR42_09395"
     CDS             420..1139
                     /locus_tag="VHR42_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX3027417.1"
                     /translation="MLKLAVCDDEISAVQDLSSKIKLFMDSRDLEYTLSQFSCGESLL
                     TCIAARNMYFDAVFLDIRMDRMNGMDTAKEIRKTNGKTNIIFVTAIKEYVFDAFDVGA
                     ANYLIKPIEAQKLQKTLDKLAKSVFLTDTHFLVIHKSGEIIKIPFPDIFYSEVVNHRV
                     FVYEQNSTYEYGAKIDDLETKLTDDFFRCHRSYIVNMRYVESYSDGFAYLTSGEKIPI
                     ATRRQPSFMKALLHYQRKEMR"
     gene            1141..2451
                     /locus_tag="VHR42_09400"
     CDS             1141..2451
                     /locus_tag="VHR42_09400"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEX3027418.1"
                     /translation="MELFRTPLRFWFGFLFSLVLCGGLQALIQCYFLIRFASAKSRLS
                     YYFIYMGISYALLAAGLILPIPENLPTVIGILVLFCFTKIILRQNNMTSVILSTLVVT
                     ANVLIEGITTPLYALLSNSLEMNPIALNLCEGIVMLVLTYLVLRFFAARYNLKTNEKS
                     KYLPVLALPLLFISIVMRLLLAFRYPPVFKDYQMAYSATVIEDFQFLSLAVTAFLCVC
                     GSLFAFEKVIGYFETERENGILESQLSMQKNYAAEAKSRYEATREFRHDFKNHLIALS
                     GLMEKGETENAKKYLERFEQISQRMSLTVSTDNTVVDVLFSEKLAYAKESGIQIELDA
                     AIPSTVTIDDFDLCAIFSNALDNAVKACNSAKGPGKKIRITARPNKGFFFIEMSNTYD
                     PGCVPKGSGIGLSVIKMIVEKYHGAIEITEEDNLFCISMILPFK"
     gene            2621..3265
                     /locus_tag="VHR42_09405"
     CDS             2621..3265
                     /locus_tag="VHR42_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027419.1"
                     /translation="MSIAISMQTEGYGKTEAGGQQPSEVDRSEQKKKKAAEQLQRVGQ
                     NNVSEEVSVPDKVAKVEPSVDQFIRSDGSETNPAGTYSLKKDGHGNSKIVYDKHATGS
                     DALNETSDKKGNPLVSPKKPDDNPNKAPENSPSNKSGGLECTVNTDKVDAEIKNLKKE
                     KQRIEQEIKQAGGDENKKSELEQRLKEIDAELQTKDTDAYRKQSATVTYKSIQK"
     gene            complement(3281..4915)
                     /locus_tag="VHR42_09410"
     CDS             complement(3281..4915)
                     /locus_tag="VHR42_09410"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HEX3027420.1"
                     /translation="MSEPVYIAGHRNPDTDSVCSVIAYAELKKRLGVPAVPVRIGNIN
                     RETGFVLNYFGVEAPEYLESVRTQVSDLNMDIISPISADISVKAAWSIMQKSNVKVLP
                     VSNDDGRLTGIVTLSDITRSYMDALESNLLSAGNTPLHNITDTLKAKLISGSEGSFRP
                     SGKVVIAAAEPDRMDPFIEKGDIVLAGNREDTQKKAVEIGASCLVLTCGAKSDRALLE
                     LAENNGCVVMETDYDTFAAARLIDQSVPVGFIMTKDGLIRFHISDYIDSIKDKMLQTR
                     YRSYPVVDDDGFIKGFISRYHLISQRRKKIILLDHNEKSQTIDGIEQAEILEVIDHHR
                     IGDIQTENPVYFRNDAVGSTSTLIAGMYFEHGIRPPKSIAGILCAAILSDTLQFRSPT
                     STYADTSIAEKLAEIAGIHIDEFSAQMFEAGSALEGMSPEEILNYDFKEYTSGQLKFG
                     IGQVNSYHPQHVEQIRDSLLQYMRGVMDHGHYDFLLLLVTDLVNEGSFLLHVGDCGAL
                     IRKAFGASAENNTVYLQGVVSRKKQIVPRLLNAIKV"
     gene            5291..5497
                     /locus_tag="VHR42_09415"
     CDS             5291..5497
                     /locus_tag="VHR42_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009677130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6199 family natural product biosynthesis
                     protein"
                     /protein_id="HEX3027421.1"
                     /translation="MMYLFWGVIVLAAGFFMLFAPKTCYDITQGWKNSSDTDPSPLFI
                     LSTRLGGILFLLAGIAGVIVSFLR"
     gene            5549..5956
                     /locus_tag="VHR42_09420"
     CDS             5549..5956
                     /locus_tag="VHR42_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3027422.1"
                     /translation="MENSKPDKSFFVDFLKNYNQAFYDKDIDKLKEFYDSTNNVLIYF
                     DNHKNNDTYCLKDHLRLISDFFQNGKATESGGVEPISIENLQIFQTTDSACLCFITRY
                     KSFPVPSIRATMYLECIDGLWKIIHVHCSFEPE"
     gene            6024..6701
                     /locus_tag="VHR42_09425"
     CDS             6024..6701
                     /locus_tag="VHR42_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3027423.1"
                     /translation="MTNILIIEDDKEINNLLCSILCKEKFNCKSIYSGLQALAALKTN
                     RFDMVLLDLMLPGMPGEKLLRDFRRFSNAPVIIISAKDETAVKVDMLRTGADDYITKP
                     FDNNEVLARVESNLRRSLGYSSPLLIAGSLEIDTEKNVCTVSGQTVSLTGIEIKILRL
                     LMEHPQKLFTKANLYSSVWNEPYDYDDNAVNTHISNLRRKLKSVCPDQEFIETVWGIG
                     YKLACPS"
     gene            6803..>7456
                     /locus_tag="VHR42_09430"
     CDS             6803..>7456
                     /locus_tag="VHR42_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3027424.1"
                     /translation="MNEPILETKGLTKKYRGTLALDHMNLTLRRGEIYGFIGENGAGK
                     TTTIRLITGLSFPTEGHLSLFGRSGEKQLQKQRERMGCMVETPALYKNMTAEQNLEAQ
                     RIQRGIPEKECIKETLKLVGLSDTGKKAARHFSLGMRQRLGIAMALLNDPEFLILDEP
                     INGLDPSGIVEIRELMKQLNREKGITILVSSHNLSELYQTATRYILLHKGRLLEELSQ
                     "
BASE COUNT         2039 a         1765 c         1724 g         1928 t
ORIGIN      
        1 atgtctgttt cttggatcat ctgccccagc aaatactgtt cgataacctc tgtaaggagt
       61 attccggtcc ggacatgctc cgaatggaaa aaagggacct gtaccttctc tgccatcaga
      121 gtatccgcaa gtcgaaactg gcaatccata tagccaaggc atttggttct gtcaccgtgc
      181 ggaattggaa tactgtaaat aaagtatatg agatgttgac ttctctgtag aaacgcgtcg
      241 tctacccttc catcacctca tacccataaa atcaagggga ctatgccttg acgagtgtta
      301 atgttttatt gtttccggaa ttcgaaaaac actgaacgct ttcggtccga tcgaggcgct
      361 ggggtgtgtc cctgagtagc agctccatta aataccgagc aaatagaaag gttgattgaa
      421 tgcttaaact tgcggtatgt gatgatgaaa tatccgctgt tcaggattta agcagtaaaa
      481 taaaattgtt catggattcc agagatttgg aatataccct gtcacagttt tcctgcggcg
      541 aatctttact tacctgtatc gccgcgcgaa acatgtattt tgacgctgtt tttctggata
      601 tccgaatgga tcggatgaac ggcatggata ccgccaaaga gatccgcaaa accaacggca
      661 agactaatat tatttttgtg acggccatca aggagtatgt ttttgacgcc tttgacgtcg
      721 gcgcggcaaa ctatctaata aaaccgatcg aagcgcaaaa attgcagaaa acactggaca
      781 agttggcgaa gtcagttttt ttaacggaca ctcactttct tgtgattcat aaaagcggtg
      841 aaatcataaa gattccgttt ccggacattt tttatagtga ggtggtaaat caccgcgttt
      901 ttgtctatga gcagaacagc acctatgaat acggagcgaa aatcgacgat ttggagacaa
      961 agctgacgga cgattttttc cggtgccaca gaagctacat tgtcaatatg agatacgttg
     1021 agagctattc cgacgggttt gcgtacctga ccagcggtga gaaaatcccc attgccaccc
     1081 gaaggcagcc ttcctttatg aaggccttac tgcactatca aagaaaagag atgcgttgag
     1141 atggagcttt tccgtacgcc tctccgcttt tggtttggct ttctcttttc tctggtgctt
     1201 tgcggcgggc tgcaagcctt gatccagtgc tattttctca ttcgattcgc gtcggcaaaa
     1261 agcaggctgt cttattactt catttatatg ggaatctctt atgcgcttct ggctgccggg
     1321 cttattctcc cgatccctga gaatcttccg accgtgattg gaatcctcgt tttattctgt
     1381 ttcacaaaaa ttattctccg gcaaaacaat atgacgtctg ttattctctc cactctggtt
     1441 gttaccgcca atgtgctgat cgagggtatc accacgcctt tgtatgctct gctgtcaaac
     1501 agccttgaaa tgaatcccat cgccctgaat ctatgcgagg gaatcgtcat gcttgtgctg
     1561 acgtatctgg ttctccgttt ctttgcggca cgatacaatc tgaaaaccaa cgaaaaatcc
     1621 aaatatctgc cggtgctcgc tttgcccttg ctttttatca gcattgtgat gaggttattg
     1681 ctggctttcc ggtatccccc ggttttcaaa gattaccaaa tggcatattc ggcaaccgta
     1741 atcgaagatt ttcaattttt gtctcttgcc gttacggcgt ttctttgtgt gtgtggttcc
     1801 ctgttcgcgt ttgaaaaggt aatcggctat tttgagacag aacgggaaaa tgggattttg
     1861 gagtcccagc tttccatgca gaaaaactat gcagcggaag caaaatcaag atacgaagct
     1921 acccgcgaat tccgccatga tttcaaaaac catttaattg cgctttcggg cctaatggaa
     1981 aagggagaaa ccgaaaacgc caaaaaatat ctggaacgct ttgaacagat ttctcaacgg
     2041 atgtccctca ccgtcagtac cgacaatacg gtcgttgatg ttcttttcag tgagaaattg
     2101 gcatacgcga aagaatccgg cattcagatc gagcttgacg ccgccattcc ttccaccgtg
     2161 accattgacg attttgattt atgcgccatt ttctcgaatg ccttggataa cgccgtcaag
     2221 gcatgtaaca gtgcaaaggg tccgggcaag aaaatccgta taacagctag accaaacaag
     2281 ggcttctttt tcattgagat gagcaatacg tatgatcccg gatgcgttcc gaaaggttcg
     2341 ggaatcgggc tgtcggtaat caaaatgatt gtggagaaat accatggggc gattgaaatc
     2401 acggaagaag ataacctttt ttgtatcagc atgattcttc catttaaata aaaaacaaag
     2461 cggctgaaag acaaaaccaa gcccggacat tcacaaagct ttaacgctct gaatgcccgg
     2521 gcttgcgttc acgcttcaaa tggcagcgct tacgttgttt tgctgaaaag ggaacctacc
     2581 ttgacgataa ataaataaaa tccgaatgga ggtatctatt atgagtatag caatttcaat
     2641 gcaaaccgaa ggatacggta aaaccgaagc gggagggcaa cagccttcag aggttgatcg
     2701 gtccgaacaa aagaagaaaa aagctgcgga acagctgcag cgcgtcggcc aaaataacgt
     2761 cagtgaagaa gtttccgttc cggataaagt cgccaaggtc gaaccgtcgg ttgaccagtt
     2821 tatccggagc gacggttccg aaacaaatcc ggctggaacg tacagtctaa aaaaagacgg
     2881 gcacggcaat tcaaaaattg tgtacgataa gcatgcaaca ggctccgacg cactaaacga
     2941 aacgtccgat aaaaaaggga atcctcttgt ttccccgaaa aaaccggatg acaatccgaa
     3001 caaggcgccg gagaattcgc cgtccaataa gagcggcggg ctcgagtgta cggtaaacac
     3061 cgataaagtc gatgcggaaa tcaaaaatct aaaaaaagaa aagcagcgga ttgaacagga
     3121 aataaagcaa gccggcggag atgaaaacaa gaaaagtgag ttggaacaac gcttgaaaga
     3181 aatcgacgct gaacttcaga ccaaggatac cgacgcctac agaaaacaga gtgcgaccgt
     3241 cacctacaaa tccatacaga aataagggct gtcgtcctag ctatactttg atcgcattca
     3301 agaggcgcgg cacgatctgc ttctttcttg acacgacccc ttgcaggtaa acggtgttgt
     3361 tttcggccga cgcgccaaac gctttgcgga tcagcgcgcc gcaatcgcca acatgcagca
     3421 ggaaggagcc ctcgttcaca aggtcggtca ccaacagcaa caaaaaatca tagtggccgt
     3481 ggtccataac cccccgcata tactgtaaaa gggaatcccg aatctgctcc acatgctgcg
     3541 gatgatagga attaacctgt ccgattccga atttgagctg cccggacgta tactccttaa
     3601 aatcgtaatt gaggatttcc tccgggctca tgccttccag cgctgatccc gcctcaaaca
     3661 tttgcgcgga aaattcgtcg atatgaattc cggcgatttc agcgagcttc tccgcaatcg
     3721 aggtgtccgc gtaggtgctt gtcggcgacc ggaactgaag cgtatcggag aggatagcgg
     3781 cgcacaatat ccccgcgatt gatttcggtg gacggattcc gtgttcaaaa tacattcccg
     3841 caatcagtgt ggacgtgctt cccacggcat cgttccggaa atagaccgga ttttccgtct
     3901 ggatatcccc gatccggtgg tggtcgatca cctccagaat ctccgcctgc tcgattccgt
     3961 cgatcgtctg gctcttttcg ttatggtcca gcaaaatgat ttttttcctt ctctgagaaa
     4021 taagatgata cctggaaata aatcctttga tgaacccgtc gtcgtccaca accgggtagc
     4081 ttctgtaacg cgtctgaagc attttatctt taatggagtc aatatagtcg ctgatatgaa
     4141 agcggatcag cccgtccttc gtcataataa aaccgacggg aacactctgg tcgatcagtc
     4201 tggcggccgc aaaggtatca tagtcggttt ccatcacaac gcatccgtta ttctccgcaa
     4261 gctccagtag ggcccggtcc gacttcgcgc cgcaggtcaa aacgaggcag ctcgcaccga
     4321 tttccactgc ttttttctgc gtatcttccc gatttcccgc caaaacaata tcgccctttt
     4381 caataaaggg gtccattctg tccggttcag ctgcggcaat aacaaccttt cccgacggcc
     4441 ggaaactccc ttcgcttccc gaaatcagct ttgccttcag agtatctgta atgttgtgca
     4501 gcggggtgtt gcctgccgaa agcagattac tctccaacgc atccatataa cttcgggtga
     4561 tatcggagag ggttacaatg cccgtcaatc tgccgtcgtc attggaaacc ggcagcacct
     4621 tgacgttgct tttctgcata atgctccacg ccgcttttac ggaaatatcg gcggaaattg
     4681 ggctgatgat atccatattc aggtcggaaa cctgcgtccg gaccgattcc agatactccg
     4741 gcgcttcaac cccgaaatag ttcagaacaa aaccggtctc ccggttgata tttccgattc
     4801 ggaccgggac cgcgggcacg ccgagtctct tcttgagctc cgcgtacgcg ataacggaac
     4861 aaaccgaatc cgtatccggg ttccgatgtc ctgcaatata aaccggctct gacatgatgc
     4921 acaacctcca atttaaaatg ccgacggctg acagaatgga actcgctcgg gccaaagccc
     4981 gcgtcctgaa tacagacctg ctgaatcata agtgtaaaat ggaaactgtt ttttcgtgag
     5041 attattttac cacgcttctt acggaaattg ttacttaagc gggttttcgc atgattcttt
     5101 taatttttac ttttcgaacc gtgaattttc tcgctcactg tgtttcggaa ccgaagaatt
     5161 tccgctttga tttaggcggt tggttacgac gcgcaggaaa aattttgacg caccggaact
     5221 ttcccatgtt ccatcttggc aatttcatga tatactgaat ttatactaaa tttcaaaaag
     5281 tgggtgtgaa atgatgtacc tgttttgggg cgtcatcgta ttggcggcag gctttttcat
     5341 gctgtttgcc ccgaaaacat gttatgatat cacacaggga tggaaaaaca gttcggatac
     5401 ggacccttct cctctgttta tcctcagtac aaggctcggc ggtattctct tccttcttgc
     5461 cggtattgcg ggtgttattg tttcgtttct gcgttagcct tcggcaagcg atcgcatttg
     5521 atattctttg atttcgtgag gtgttgttat ggagaattcc aaaccggaca aatcgttttt
     5581 tgttgatttc ttgaagaatt ataaccaagc cttctatgat aaggacattg acaagctaaa
     5641 agagttctat gattctacca ataatgtcct gatctacttc gacaatcaca agaataacga
     5701 cacctactgc ttgaaagatc atctgcggct catctccgac ttttttcaga acggaaaggc
     5761 aacggagtcc ggaggagtgg aaccgatcag cattgagaat ttgcaaatct ttcagacaac
     5821 ggattccgcc tgcttgtgct tcataaccag atacaaaagc tttcctgttc cctcaattcg
     5881 agcaaccatg tatctggaat gcatcgacgg cctgtggaaa atcatccatg ttcactgttc
     5941 ttttgaaccg gaataatagc cgcataaggt catctgtttg cacagcatct aatgaaaagc
     6001 actgaagcat aggggacaat tgcatgacta acattcttat tatagaagac gataaagaaa
     6061 ttaacaattt attatgcagc attctttgca aagaaaaatt caactgcaaa tccatctatt
     6121 ccggtctgca ggcattagca gcattgaaaa caaaccgatt cgatatggta ctgctggatt
     6181 tgatgcttcc cggaatgccg ggcgaaaaat tgctccggga ttttcgtcgc ttttcaaatg
     6241 ctccggtgat tattatctcc gccaaggatg aaacagccgt gaaggtcgat atgctccgca
     6301 cgggcgccga cgattacatc acaaagccat tcgacaacaa cgaggttctg gcaagggtcg
     6361 aaagcaatct gcggcgcagt ctggggtatt cctccccgct cctgattgcc ggttctctgg
     6421 aaatcgacac ggaaaaaaac gtctgcactg tctccggcca aacggtttca ttaaccggga
     6481 ttgaaataaa aattcttcgg cttctcatgg aacatccgca gaagctgttt acaaaggcca
     6541 atctgtacag cagcgtctgg aacgaaccgt atgattatga cgacaacgcg gtcaatacgc
     6601 acatcagcaa tttaaggcgg aagctaaaat ccgtctgccc ggatcaggaa tttattgaga
     6661 ctgtttgggg aatcggctat aagctcgcct gtccctcctg aaacacctgt ttccgtaaat
     6721 acggaaacag gtgtttttat cataatcttt agaaacgctt aagaattgca atttataata
     6781 ggcggtaaag gagatgggac ttatgaatga acccatattg gagacaaaag gactaaccaa
     6841 aaaataccgc ggcactttgg cgcttgacca tatgaacctt accttgcggc gcggtgaaat
     6901 ttacggcttt atcggagaaa acggcgcggg caaaaccaca accatccgtc tcattaccgg
     6961 actatctttc ccgacggaag gacacttgtc gctgtttgga agatcgggtg aaaagcagct
     7021 gcaaaagcag cgcgaacgca tgggatgtat ggttgaaacg cccgcattgt ataaaaacat
     7081 gaccgccgaa cagaatctgg aggcacagcg aattcagcgg ggcattcccg aaaaggaatg
     7141 catcaaagaa acgctgaaac tggtggggct gtcggatacg gggaaaaagg ccgcacgcca
     7201 tttttcgctc ggcatgcgcc agcgacttgg aattgcgatg gcattgctga acgacccgga
     7261 atttttaatt ctggacgaac caatcaacgg ccttgatccg tccggaattg ttgaaatacg
     7321 ggagctgatg aaacaactga accgggaaaa aggcattacg attcttgttt ccagccataa
     7381 cctcagcgaa ctgtatcaaa ctgccacccg ttacattctg ctgcacaagg gccgtttgct
     7441 ggaagagtta tcgcag
//
LOCUS       DATALY010000344         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_74373,
            whole genome shotgun sequence.
ACCESSION   DATALY010000344 DATALY010000000
VERSION     DATALY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74373"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /gene="hisH"
                     /locus_tag="VHR42_09435"
     CDS             <1..243
                     /gene="hisH"
                     /locus_tag="VHR42_09435"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617067.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX3027425.1"
                     /translation="SLNIRKPCPLLDGIEEGSYFYFVHSYMAFTDEQYLSAVADYGVS
                     VTALVQRGNVYGAQFHPEKSGEAGLLMLKNFCSLSD"
     gene            271..999
                     /gene="hisA"
                     /locus_tag="VHR42_09440"
     CDS             271..999
                     /gene="hisA"
                     /locus_tag="VHR42_09440"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX3027426.1"
                     /translation="MIILPAIDLKDGKCVRLLKGEFDTVSKVAEDPFETASRFQDAGA
                     VWMHMVDLDGAKSGIRLNRELILKIVKSVGLKIELGGGIRDRACVEDYLESGVSRVIL
                     GSAALSDPDFVKTAVRDYGERIAVGIDARNGKVATHGWLKTSETTFREFAKSMEHIGV
                     KVIIFTDISRDGMLKGPNYTQLAEIQSAVSCRIIASGGVRDLGNIQKLREMGLYGAIC
                     GKSIYSGTLDLKSAVELGGSQNAC"
     gene            989..1747
                     /gene="hisF"
                     /locus_tag="VHR42_09445"
     CDS             989..1747
                     /gene="hisF"
                     /locus_tag="VHR42_09445"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX3027427.1"
                     /translation="MLAKRIIPCLDVDMGRVVKGVNFIDLQDVGDPVECAALYDRQGA
                     DEIVFLDITATHEGRGTMADVVRRTAKKVFVPLTVGGGIRTIDDFREILRAGADKVSV
                     NSSAVRDKELVARAADKYGSQCVVVAIDARRSPDGSFHVVINGGRIDTGLNALDWAKE
                     VENLGCGEILLTSMDADGTKNGYDSELTGAVCDRVRIPVIASGGCGKLEHFSEVFEQT
                     GADAALAASLFHYHELTISQVKSHLKDRGIPVRL"
     gene            1785..2111
                     /gene="hisI"
                     /locus_tag="VHR42_09450"
     CDS             1785..2111
                     /gene="hisI"
                     /locus_tag="VHR42_09450"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX3027428.1"
                     /translation="MQNLKNFFLKSELIPAVIQQDDTGEVLMLAYMNEESLKKTLETG
                     YTWFFSRSRQKLWMKGETSGHVQRVISICGDCDDDTLLVKVEQTGPACHTGSHSCFFN
                     AIFEKK"
     gene            2148..>2467
                     /gene="hisE"
                     /locus_tag="VHR42_09455"
     CDS             2148..>2467
                     /gene="hisE"
                     /locus_tag="VHR42_09455"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX3027429.1"
                     /translation="MDDVFEKLYQVVEERKLHPQEGSYTCYLFEKGLDKILKKVGEEA
                     SETIIAAKNGVREDTVNEISDLLYHLVVMMADQNIRLEEIEAVLKERSSKIGNLKTPH
                     QSNP"
BASE COUNT          654 a          543 c          712 g          558 t
ORIGIN      
        1 agtcttaaca tccggaagcc ctgcccgctt ctcgacggaa tcgaagaagg aagctatttt
       61 tattttgttc actcctatat ggcttttacc gatgaacaat atctttcggc cgtcgcggat
      121 tacggagtaa gcgtcaccgc gctggttcaa aggggaaatg tctacggcgc gcaattccat
      181 ccggagaaaa gcggcgaagc aggtcttttg atgctgaaaa atttctgctc cctttccgac
      241 taaaacaagg gaaaaacagt ggaggtcaaa atgataattt taccggcgat tgatttaaaa
      301 gacggaaaat gcgtcagact cctgaaaggc gagttcgaca cggtttcaaa ggttgcggaa
      361 gacccctttg agacggcgtc gcggtttcag gatgcggggg ccgtgtggat gcatatggtt
      421 gatctggatg gagcaaaatc ggggatccgg ctcaaccggg agctgattct taagattgtg
      481 aagagcgtcg gtctgaaaat tgagcttggc ggaggcatcc gcgatcgggc ctgtgttgag
      541 gactatctcg aaagcggggt ttcgcgcgta atactcggct cggccgcgtt gtccgacccg
      601 gatttcgtca aaaccgcagt ccgggattac ggggaacgca tcgcggtcgg tatcgacgcc
      661 cggaacggaa aggtcgccac tcacggttgg ctgaaaacaa gcgaaaccac gttccgggaa
      721 ttcgcaaaat ccatggaaca tattggtgta aaggtaatta tctttacaga tataagtcgg
      781 gacggaatgc tgaaaggccc taactacaca cagcttgctg aaattcagag tgcggtatcc
      841 tgccggatca tcgcaagcgg cggggtcagg gatctcggta atattcaaaa gcttcgcgaa
      901 atggggctat acggcgcgat atgcggaaaa tccatttaca gcggaacgct ggatctgaaa
      961 tccgccgttg agttgggagg cagtcagaat gcttgctaaa agaatcatcc cctgtctgga
     1021 tgtggatatg ggccgggttg taaaaggagt taattttatc gatcttcagg atgtcggcga
     1081 cccggtcgaa tgcgctgcgc tttatgaccg tcagggcgcc gatgaaattg tttttctcga
     1141 tattaccgcg acacatgagg gccgcggtac aatggcggat gtcgtcagga gaacggcgaa
     1201 aaaggtgttt gtacctctga ccgtcggagg aggcattcga accatcgacg atttcaggga
     1261 aattttacgc gcgggggcgg acaaagtttc ggtaaactcc tccgcagtca gggataaaga
     1321 gctggtggcc cgggccgccg acaaatacgg cagccagtgt gtggtggtgg ccatcgacgc
     1381 gcgccggagc ccggacggaa gctttcatgt cgtgatcaac ggcggaagaa tcgacacggg
     1441 actgaacgcg cttgattggg ccaaagaagt ggagaatctc ggctgtggcg aaattcttct
     1501 gacttcgatg gatgccgacg gaaccaaaaa cggttacgac tcggagctca caggggcagt
     1561 ttgcgatcgg gtgcgtattc cggtgatcgc ctccggcgga tgcggaaaat tggagcattt
     1621 ttccgaagtg tttgagcaga ccggcgccga cgcggcgctg gcggcctcac tgttccatta
     1681 tcacgagctg acgatcagcc aggtgaagtc gcacctgaaa gaccggggaa ttccggtcag
     1741 gctttaatcg cgcgttctta aaaatccgct gtcgaggtga ttagatgcag aatcttaaaa
     1801 acttttttct aaaatccgaa ttgataccgg ctgttattca gcaggatgac acgggcgaag
     1861 tgctgatgct tgcctatatg aatgaagaat cgttgaaaaa aacgcttgaa acgggttata
     1921 cctggttttt cagccgctcc cgccagaagc tatggatgaa aggggaaact tccggtcatg
     1981 ttcagcgcgt aatcagcatt tgcggcgact gcgacgacga tacgttgctg gtcaaagttg
     2041 agcagaccgg tccggcatgc catacgggca gtcattcctg cttttttaat gcgatatttg
     2101 aaaagaaata aaaataataa aattagtatg actaaggtgg tattgagatg gacgacgtat
     2161 ttgaaaagct ttatcaggta gtggaagagc gaaaactgca cccgcaggaa ggctcttata
     2221 cctgttatct gtttgaaaaa gggcttgaca agattctcaa aaaagtgggc gaggaagcct
     2281 cggagacaat cattgccgcg aaaaacggtg taagggaaga taccgtcaat gaaatcagcg
     2341 acctccttta tcatctggtg gtcatgatgg cggatcagaa tatccggctt gaggagatcg
     2401 aagcggtcct gaaagagaga agcagtaaaa tcggcaactt aaaaactcca caccaaagca
     2461 atcccaa
//
LOCUS       DATALY010000345        19923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_159767,
            whole genome shotgun sequence.
ACCESSION   DATALY010000345 DATALY010000000
VERSION     DATALY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19923
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159767"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1163)
                     /locus_tag="VHR42_09460"
     CDS             complement(183..1163)
                     /locus_tag="VHR42_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3027430.1"
                     /translation="MPRIGDLPLEIQEIKSLCIRIRENVSKVIVGKDRLIDLMTTALL
                     SGGHVLLEDVPGTGKTMLAKSLAKSLGTDFKRVQFTPDLLPSDLTGINYFNQKINDFI
                     FRQGPVFTNILLADEINRATPRTQSSLLECMEEKQITIDGTTYRLSEPYFVIATQNPV
                     ETQGTFPLPEAQLDRFLIKSEIGYPTANETEEILEKFQRANPFDEITPVCTAEEITEA
                     ANSCRNVYISDDMRKYIIEIMEKTRIHPSVELGVSPRGAIALLRACQSFAALNGRSFI
                     IPEDVKHLVPYVFGHRLILKNDYRIRRMKPESVIEGIINEVKAPTENWEK"
     gene            1385..2077
                     /locus_tag="VHR42_09465"
     CDS             1385..2077
                     /locus_tag="VHR42_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3027431.1"
                     /translation="MESLKILVVDDDKNINELLRLYLEKEGYLVVQAFDGRGAIRKFE
                     EQNPNLVLLDIMLPGMDGWQVCREIRKKSDKPIIMITAKGETFDKVLGLELGADDYVV
                     KPFDTKEVVARIKAVLRRYSSSGDNSEVKEVRYDRLVINLTNYELIIDERTIDTPPKE
                     LELLYHLASNPNRVFTRDQLLDEVWGFEYYGDSRTVDVHVKRLREKLEGVSDKWSLKT
                     VWGVGYKFETKQ"
     gene            2061..3533
                     /locus_tag="VHR42_09470"
     CDS             2061..3533
                     /locus_tag="VHR42_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3027432.1"
                     /translation="MKQNSNSIYLRLFRDKSVFKKYFITCTAIMLSCFLFLGTVLIIF
                     SVNYSNDQRQSTNFNCARAIKNLYTNILTNNFIPSSDQGMSAILDYSQQLTSLIGEVD
                     SYIVDSKGNVLGHSQQSFTRTVLNRVPQSIMDSFSKGKKEYREIGTLGGFFDSSYNTV
                     GVPIVVENQIVGAVFVSSRANNLLKYMYDMFRMFLLCIFISLVFSFVIIYFVTAKMVK
                     PLRDMSRAAKSFSKGDFSIRVPVEDKDEIGQLAVAFNNMATSLTSLEEMRRSFVANVS
                     HELKTPMTSIAGFIDGILDGTIPENKQEYYLTIVSNEVKRLSRLVRSFLDIASIESGE
                     IKINPSEFDVAETVRRVIVGFEYTINEKKLDIRGLDSDWQFKVVSDSDLTYQIVYNLV
                     ENAIKFANENGYIEIKLAEKNKKVYISVKNSGMGIPQGELPYIFDRFYKTDKSRSLDR
                     KGVGLGLYIVKSVLNLQGQDILVKSVEGEYCEFVFTLAKA"
     gene            3627..5078
                     /locus_tag="VHR42_09475"
     CDS             3627..5078
                     /locus_tag="VHR42_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3027433.1"
                     /translation="MNQFDDDGNNTEQNSGASGQNGNTPEQNSGTPEKNDNMSSYGGN
                     VEYKWNYDDFQKALVESKAPKEKKKMTKGFKAFIISICSVFAVAVLGLAGIGIRDLAL
                     NNSQNGTGTSSSGGNVKASSGPSLTITNTPSASSASTTALSGQLTTAQINKKVSPSVV
                     GVISYSLQSVEAAAEGTGIIMSPDGYIITNEHVIDGADKVSVVLSDKTEIAAKVVGKD
                     LRTDLAVLKITKTGLTAATFGDSSKLQVGDTAVAIGNPGGVELADTVTQGIISALNRT
                     VTSENGYTQSCIQTDASINPGNSGGPLVNVYGQIVGITSSKIADVDYEGIGFAIPINT
                     AKPIIDDLIKYSYVQGRVKLGISVKELSSYQAKIYGYEAGLRITAIEKTSDAYKQGLQ
                     QNDVITKINGISVTSYDQFYQEESKYKAGNSVTLTIFRYSSGQSKNFTVKVKLLEDKG
                     TTSTTTTSSSSSSSDSSSGSYTDPFSYFNQGNN"
     gene            5398..6948
                     /locus_tag="VHR42_09480"
     CDS             5398..6948
                     /locus_tag="VHR42_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX3027434.1"
                     /translation="MFDKIKARINIWKAQGPSKAVGDTVPDPEHVKHNMVDNLIYFQG
                     KFAGCADFICKEMTVCGNKVALMMIDNMVDKVALTESVMNPLTRAQKPNAPMNEEETY
                     LWVRDCVLASIDQKEIFTLEECISLLMLGFVIVLFGDEDKAISIGLQGFKYRGIEEPN
                     LEQVLRGSREGFVEPLRINMSMVRRRVKNPNLKFEVVTIGNESKTDICLTYVKGVASE
                     SILSDIRHRLLSIDISMVLASGYIQAYFQNNPYSIFATVGTTERPDTLCGKLNEGRVG
                     IIVDGTPLALYTPFLFVENFQNMDDYAVGPYYAAFTRLLKFMAFFTSVFVPGLYVAIG
                     SFHQALLPTPLLVTLAKAEETTPFPLVLEALLMQFIYELIREAGLRLPKQFGFAINIV
                     GALIVGEAAVTAGLIGAPMVIIVAITATTSLVTPTLYEPGVILRFLFIILAGMSGLFG
                     ITLGTALILFHLCSIKSYEIPFTAPLAPFSLFSMRDLLIRAPWKILSRRKMSVQNMVG
                     SNVDKNLE"
     gene            6958..8061
                     /locus_tag="VHR42_09485"
     CDS             6958..8061
                     /locus_tag="VHR42_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HEX3027435.1"
                     /translation="MEKSSVISVKQLLLVMVILRIAFSTSYISGLNTGNSVQDVLVAI
                     PVLFLISFIVALPILILLKRHPGKAIMECSIQVMGRGVSAVIGLIYISFFIFQAAAEL
                     GTFHLVFTNAVIPESQEYAISIPLLIVCMYGAAKGIESVSRFGTFVFALYVLILIFNM
                     ISLLPRAQMGYLFPLFYNGPRVFLQSLNSMFNSSVQILTLAVCASFLKPGSNVRKVFS
                     YYNVFASLLLFLYTFLAVMVMGPYTSKQVFPLFTLSELSKVGVFERLDSLEMVLWIFD
                     TILAVTVYIFLAANMPTKFGLNKYRKVVIVLIGAAVYFLAPVISHNYLMYQSCMGSLY
                     ATAIVVTLIVIIPLIILITDIIKEKVKKNEAEN"
     gene            8045..9190
                     /locus_tag="VHR42_09490"
     CDS             8045..9190
                     /locus_tag="VHR42_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX3027436.1"
                     /translation="MKRKISFLLCCCAAAMLFSGCSPYKELKELSIVQGMGVDSGQNG
                     GYNLTFQVLKSKTSNNSGSQSGGGGGENEVYVSSGTTLFDTIRNTTLQMGRKLYFPST
                     QIFVIGEEESKKNLVELFDFMQRNHDMRPTVKVYVSRGNANGILTATKNGSQIPAADI
                     AEISKSYYNSSKTIDVTFSDIYMQLANGITDPVIPVISAANDNTGKSMVEMQGSAVFN
                     KNVLAGYLDDSQTRGYLWIMGKAESGIVVVYPSAGGKVSLEVTKNKSDVKIAQDKNGQ
                     AIVKITIDVSSNMGEVQGTYSSMGMEYKQELIRLQAEVIKKEAQSAIDGALRAYNSDI
                     FGFGMKIFQSKPDEWKKLSKNWDTDAHKIPVEITVKSEIRQRGIISK"
     gene            complement(9257..9712)
                     /locus_tag="VHR42_09495"
     CDS             complement(9257..9712)
                     /locus_tag="VHR42_09495"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX3027437.1"
                     /translation="MARDFRSKIAAGDNFPLHLTLFLLCLVPLVLSVILHTDGVRTTF
                     EWNGHFQPVGTFCLFREATGYQCPVCGMTRCFIYLSHFQFRNAWAMNPAGIAVYLLCI
                     FEACYRLFLILSRRATGRKEIRYLEKAVVTLTLLAVSVHFMDQFLPNRY"
     gene            complement(9702..10310)
                     /locus_tag="VHR42_09500"
     CDS             complement(9702..10310)
                     /locus_tag="VHR42_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX3027438.1"
                     /translation="MKTCPKCGSACEDADIICRNCGYLLGNDHQASENPTPDTSAQSS
                     GQNAQNPENQQPDLGVPPQNATNVPQNPGYPPQPGPQGGAPGYDPRYSYQDPYLAPKN
                     DSFAIASLVLGITGVVTVFCCIGIIPCILAVIFGFISNNRIKKSGGALKGKGMAIAGL
                     VLGFVGVGIFIKMLISLAIGGFNWEEFRRQVETQMQKSRYGS"
     gene            complement(10365..10841)
                     /locus_tag="VHR42_09505"
     CDS             complement(10365..10841)
                     /locus_tag="VHR42_09505"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX3027439.1"
                     /translation="MKECPVCRKTGADSDTYCKYCGCPFPPPQKEQNEYAGYQTFPGQ
                     PYYGYTPYRQYPPEQSYSGLAIASMILGILSLPLFCFYGSGVITAILSIIFGFVAKSK
                     ISTGASKGFGMAITGIVLGIVTLTIGIIVVLLISLSLISLSGMGNYFNQYSSSAGI"
     gene            complement(10943..11620)
                     /locus_tag="VHR42_09510"
     CDS             complement(10943..11620)
                     /locus_tag="VHR42_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX3027440.1"
                     /translation="MSNYEIVLFDVDGTLLDTKPGILRCVKKALTGLGIPIPPESKLN
                     LFMGPPIKYCFTDVCGLDEETAEQAIRLFRADYEETGLYQSTVYDGVRPLLENLSFRG
                     LRLGVATSKAEHMAATVLKYHGLSDYFEAVGGAPRLNYQSTKADSIRSALRDMNAPLN
                     RKTVLVGDRKFDAEGAKQTGIDNIGVLYGYGPREEILSCFGQYAATTSELEKLILDET
                     PTNSTEF"
     gene            11966..13348
                     /locus_tag="VHR42_09515"
     CDS             11966..13348
                     /locus_tag="VHR42_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3027441.1"
                     /translation="MQKKNYDVVIIGGGIGGLMCAYRIAQNSPGTTVALIEKGQDLKK
                     RSCPIVTGKSKKCINCASCAIMEGLGGAGAFSDGKYIISCEYGGWLPDLLPHETVIGY
                     IEQADRILVSFGATTERFSPDNELKLKCLRHDLHMQQAVVKHLGTDANYDTMLKMVEE
                     VAKSTEIVSETEVVDVDRQSHKILLRHKGGEEEITADRIIFAVGRAGSRFFREWCGKN
                     GIGLQNNQVDVGVRVELPAIIWDEFSKKIYEPKIWYRSKEYGDISRMFCFNERGNVVT
                     ENTQGVLTVNGHAYKDPAKKTENSNFALLSTIRFTSPFREPIEYARHVASLANLISGG
                     SVLVQRLGDLELGRRTDEKRLRACTTRPTFDAVAGDLSLCMPKRQLDNIIETLHALDQ
                     VAPGTANSDTLLYGVECKYYSARPDSEEFEIKGCEHIYAIGDGAGFTRSLSQASANGL
                     YVADRITKGN"
     gene            complement(13673..15046)
                     /locus_tag="VHR42_09520"
     CDS             complement(13673..15046)
                     /locus_tag="VHR42_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEX3027442.1"
                     /translation="MQNLEKAVSDFAGILWSWPMIAALFGTHIYLTFKLRGIQLHVFK
                     AIRLSVSKEEHGVGDVSPFSSLTTALAATIGTGNIVGVATAIVSGGPGAVFWMWISGI
                     FGMSTKYAESLLSVKYRVKMPDGSMAGGPMYVMERGLHKKWMGMLFAFFTAIAAFGIG
                     AMVQANSISGMMLKTCAIPPWVTGVTVAVLTSLVIIGGIKSISKVCSYLIPVMGLFYI
                     LSNLLILGLQWYSIPHTIQLIVTSAFSGQAALGGFSGAAAKEACRYGISRGLFSNESG
                     LGSEPIVAAAARTGNPVRQALVSYTGTFWDTVILAAVTGIMIVNSGLWTQTSDGGTLT
                     GEVFAAIPVIGPVMLSLALLTFTFATCIGWSFYAQKAVEYLLGPRAVRPYQFLYVLLI
                     FIGSVFSIGAVWNFSDMANAFMCIPNLFSILALRNVVVKETDQYLWHPRPQSSGNRRY
                     SARRSRP"
     gene            15211..16512
                     /gene="eno"
                     /locus_tag="VHR42_09525"
     CDS             15211..16512
                     /gene="eno"
                     /locus_tag="VHR42_09525"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX3027443.1"
                     /translation="MKETLPIEQVHAREIIDSRGNPTVEAEVMLEGGAVGCAAAPSGA
                     STGVFEAHELRDLDQSRFLGRGVRKAVDSVNGEIAGAVRGLDSLSQSQLDNVMIQLDG
                     TPNKSRLGANAILAVSLAGCKAAASALGLPFYQMLGGVDARTLPVPMMNILNGGAHAS
                     NNVDIQEFMIMPVGAPCFSEGLRWCAEVFHHLKKVLSDKGMATSVGDEGGFAPNLGDD
                     EDALNVILEAVEKAGYQAGNQVKIAIDAASSEWYQKDGTYLLPKKNKKLQRGELIEYW
                     KRLAENYPIISLEDGVAEEDWDGWRDLTTALGEKIQLVGDDLFVTNTQRLQKGISMKV
                     ANSILVKLNQIGTLTETMEAVRTAQRAGYTTVISHRSGETEDTTIADLAVAVNAGQIK
                     TGAPSRSDRVAKYNRLLRIEEELGCAAIYPGKCAFYNLPSA"
     gene            complement(16616..17299)
                     /locus_tag="VHR42_09530"
     CDS             complement(16616..17299)
                     /locus_tag="VHR42_09530"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX3027444.1"
                     /translation="MARIGIISAMPVEMEGFIKDLNAKEVPKKGFYQLYKGNAAENEI
                     YLVCCGIGKVNAAISTQRLIDLVGVDYIINMGIAGGIGKHLKTLDVVIGTDVVYHDFQ
                     PPDLLDKYYPFTSVFECNPQMVELAEKVCAGTREVDYFCRGRIATGDRFVEAGEDKKR
                     ILEMGGMCCEMEAAAIAHAACVNNVPFLIIRSISDLADEGAQMSYNEFEKKASVQANR
                     VVMGMINQL"
     gene            17653..19422
                     /locus_tag="VHR42_09535"
     CDS             17653..19422
                     /locus_tag="VHR42_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3027445.1"
                     /translation="MMSSWHNFFYISYDPGGFVSVDKPPIALWIQVACAKIFGIHSWS
                     LMLPEALAAVGSVAVLYHLTKRIFGYLAGIIASFALAITPIFIAVSRSNNPDAILVFL
                     LVLAAWQLIEAANKGNLKHLILAAVLIGLGFNTKMLVAFLVVPSFLLVYLFASYIKLL
                     KKCIHLVIFGMVLLAVSFSWVIAVDLTPVSQRPYVGSSSTNSEMNLVFGYNGFSRVTQ
                     YNFTSENGLNAGNHAAESSSSMKSPGSMGNFKLFNIYFGQQINWFAALAAFGVLSALF
                     YCKSLSGEEKNQKLRYLLLWGSWVFIMYLFFSVYQNMTHRYYTAVLAPGLASLVGIGI
                     SCFRKMRRHPFMALAFPVTLIISAGYQLFIVSVYHSLARVLTPILIAGIFFAVILIGI
                     AFFKKKNLGAKKCSRILSICCLAILFLAPAYWSFTTVIGRVPQADPYAGPGLLTKALP
                     DFQSIVKKLSLNTSDLKIPDYYKQTANYLEAYQESAEYLAAVPSASLMSEYLILLTGK
                     PVMNIGGYSGTNAILTLDQFKQKVSSGKLKYFITDSHADSSVNTDIINWVVENGKKVN
                     TNITDKTKTSSNYSIYDISVIYS"
     gene            19454..>19923
                     /locus_tag="VHR42_09540"
     CDS             19454..>19923
                     /locus_tag="VHR42_09540"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEX3027446.1"
                     /translation="MINGMKQDIHNSLIEQVRISLLKIIKSSVSDFTIESNDAGSDVL
                     IMAIHGGKIEPGTTELARNLSRKYNYNYYSFVGKKSNHNASLHITSTDFREPKAIEMS
                     ARSSATISIHGCAGSNQFTYIGGRDDDLGMKIKAALEKNGFTVLAAAPGLSGKS"
BASE COUNT         5359 a         4375 c         4889 g         5300 t
ORIGIN      
        1 ggtaacagtc tgataaagct cgcttcggtc accctccgtc accgattctt ctgaaaaatc
       61 catgctgata ttcagatttt tgctccagtt gctgcgatag acttttgtca gaagatcaat
      121 caggaggtaa gccatcagga tgaagacaaa tagatacata tgtttctccc caataaagcc
      181 ttttatttct cccagttctc tgtgggagct ttgacttcat tgatgattcc ctcaataacc
      241 gattccggct tcatacggcg gatccggtaa tcatttttga ggatcagcct gtgtccaaat
      301 acataaggga caagatgttt tacatcttcc gggataatga agcttctgcc gttcagggcg
      361 gcaaaagact ggcatgcccg aagcagcgca atggctcccc ttgggctgac gccaagctca
      421 acagagggat gaatccgggt tttttccatg atttcaatga tgtatttgcg catgtcatca
      481 ctgatgtaaa cattgcggca gctatttgcg gcctcagtga tttcctcagc cgtacagacc
      541 ggcgtaatct cgtcgaacgg atttgcgcgc tggaattttt cgaggatttc ttcggtctcg
      601 tttgcggtcg gatagccaat ttcggatttg atgagaaaac ggtcgagctg tgcttccggc
      661 aaaggaaacg ttccctgcgt ttcgaccggg ttttgcgttg caattacaaa ataaggctcg
      721 ctgagccggt aagtcgtccc gtcaatagta atctgctttt cttccatgca ttccagcaga
      781 ctggactggg ttctcggtgt agcgcggttg atctcgtccg ccagcagaat gttggtgaac
      841 accggcccct gacgaaaaat aaaatcattt attttttggt taaaataatt aatgccggtc
      901 agatcagacg gtaataaatc cggcgtaaac tgaacccgtt taaaatccgt tccgagcgac
      961 tttgcaagcg attttgcaag catggttttg ccggtgccgg gaacgtcctc aagcagaaca
     1021 tggccgccgg ataataaggc cgtagtcatc aggtcgatta aacggtcttt tccgacaata
     1081 acctttgaga cattctccct gatacggata cacagggatt tgatttcctg gatttccaat
     1141 ggcaggtctc ctatccgggg cattcgcccg ataaatctgt ttatcctcat tttaatacca
     1201 gaaatttcat aattcaatta aaattttctg atttgtccgg gaaatttggt taatttaaaa
     1261 atttgttcaa cattcctttg gaatatggtt tataattaga ctattaagaa tagggcgctg
     1321 cccgggcgaa aaatcgcagt gactgtcccg gaatttcatc aaaaactgta aggggcgggg
     1381 tttaatggaa agtttaaaga ttctggtagt agacgatgat aagaatatca acgagctctt
     1441 gcggctttat cttgaaaaag agggctatct ggtcgtgcag gcctttgacg gacgcggcgc
     1501 gatccggaaa tttgaagagc agaatccgaa tctggttctt ctggatatta tgctccccgg
     1561 aatggacgga tggcaggtct gccgggaaat tcggaaaaag tcggataaac cgatcataat
     1621 gatcacagcc aagggtgaaa cctttgacaa ggttctgggc cttgagctgg gggcggacga
     1681 ttatgtagtc aaaccgtttg atacgaagga agttgtcgcg aggatcaagg cggttttgcg
     1741 gaggtattcc tcgagcggag acaattccga ggtaaaagag gttcgttatg accgtctggt
     1801 aatcaatctg accaattatg agctgattat cgacgagcgg acaatcgata ccccgccgaa
     1861 ggaactggag ctgctctatc atctggcgag caaccccaat cgcgtgttta cccgggatca
     1921 gcttctggat gaggtatggg gctttgaata ttacggcgat tccaggacgg ttgacgtcca
     1981 tgtcaaaaga ctgagagaaa aattggaagg cgtgtcggac aaatggtcgc tgaaaaccgt
     2041 ttggggagtg ggctataaat ttgaaacaaa acagtaattc gatctatcta cggcttttcc
     2101 gcgataaaag cgtatttaaa aaatatttta ttacctgcac ggcaattatg cttagttgtt
     2161 tcttgttttt gggaacggtt ctgatcattt tttcggttaa ttattccaac gaccagcgac
     2221 agagtaccaa tttcaattgt gcccgggcaa ttaaaaattt atataccaat attttaacca
     2281 ataattttat acccagttcc gatcagggca tgtcggcgat tctggattac tctcaacagc
     2341 tcacgtctct gatcggcgaa gttgattctt atattgttga cagcaaagga aacgtgctcg
     2401 gtcattccca gcaaagtttt acgcgcacgg ttttaaaccg ggttcctcag tcgattatgg
     2461 acagtttcag caaaggaaaa aaggaatata gggaaatcgg aacactcggg ggattttttg
     2521 attccagcta caacacggtc ggtgtaccga ttgttgttga aaatcagatc gttggagcgg
     2581 tttttgtttc ttccagagcg aataacctgt taaaatatat gtatgatatg ttcaggatgt
     2641 ttcttctttg tatttttata tctctggttt tttcatttgt gatcatctat tttgttacgg
     2701 caaaaatggt caaacctctt cgcgatatga gccgcgcggc gaaaagtttt tccaaggggg
     2761 atttttccat tcgcgttccg gtggaggata aggatgaaat cgggcagctt gcggttgcat
     2821 ttaacaatat ggccacatcg ctgacttctc ttgaggaaat gcgccggagt tttgtcgcga
     2881 atgtttcgca tgagctgaaa acgccgatga cttccatcgc agggtttatc gacggcattt
     2941 tagacggcac gattccggag aacaagcagg aatattacct gacgatcgtt tccaacgaag
     3001 tcaagcggct gtcgaggctg gttcgttctt ttctggacat cgccagtatc gagtcgggtg
     3061 aaattaaaat caatccctcg gaatttgacg tggcggaaac cgtcagaagg gtgattgtcg
     3121 ggttcgaata taccattaat gaaaagaaac ttgacatccg gggccttgat tccgattggc
     3181 agtttaaggt cgtttccgat tctgatctta cctatcagat tgtttataat cttgtggaga
     3241 atgcgattaa gttcgcgaat gaaaacgggt atattgaaat taagcttgcc gaaaagaaca
     3301 aaaaagtcta tatcagcgtt aaaaacagcg gtatgggaat cccgcagggc gagctgccgt
     3361 atattttcga tcgtttttac aaaacagaca aatcccgtag ccttgaccgc aaaggcgttg
     3421 ggctcgggct ttatatcgta aaatcagtgc tcaatcttca gggacaggac attttggtca
     3481 agagcgtgga gggcgaatac tgcgaattcg tttttacact tgccaaagcc tgagcctatt
     3541 tcaaatttat ttttactgca tttaaaatat attcatattt atatgataaa tttagatcac
     3601 accccagata acaggaggtt ttcgatatga atcaatttga tgatgacggc aataataccg
     3661 aacaaaacag cggagcttcg gggcagaacg gcaacactcc ggaacaaaac agtggaacac
     3721 cggagaaaaa tgacaacatg tcttcctatg gaggcaatgt tgagtataag tggaactatg
     3781 acgattttca aaaggcgctg gtggagagca aggctccaaa agagaaaaag aagatgacca
     3841 aagggtttaa agctttcatc atcagtatct gctcggtgtt tgccgtagcc gtactgggtc
     3901 tcgcgggtat cggaatacgg gatcttgcgc tgaacaacag tcagaatggc accggtacct
     3961 cctcttccgg cggcaatgta aaagcgtctt ccggcccaag tcttacaatt acaaacactc
     4021 cgtcggcgtc atcggcatcg actacggcgc tttcgggtca attgacgacg gcccagatca
     4081 acaaaaaagt atcgcccagc gttgtgggcg tgatttccta cagcctccag tcggttgagg
     4141 cggctgcgga aggcaccggc atcatcatgt cgcctgatgg atatatcatc acaaatgagc
     4201 atgtaatcga tggtgcggat aaggtttctg ttgtgctttc ggataaaacc gagatcgccg
     4261 cgaaagtggt tgggaaggat ttaagaaccg acctcgctgt tcttaaaata accaaaaccg
     4321 gattaaccgc ggccacattc ggcgattcca gcaaacttca ggttggtgat accgctgttg
     4381 ccataggcaa tcccggcggc gttgagctgg ccgatacggt aacccagggc attatctccg
     4441 ctctgaaccg aacggtcacc tccgaaaacg gatataccca atcctgcatc cagaccgacg
     4501 cgtccatcaa ccccggcaat tccggcgggc cgctcgtgaa tgtttacggg cagattgtcg
     4561 gcatcacttc ttcaaaaatt gccgatgtgg attatgaagg catcggattt gccattccga
     4621 tcaatacggc caaaccgatt atcgacgacc ttatcaaata ttcctatgtt cagggcagag
     4681 tcaaactggg catctcggtg aaagagctga gttcatatca ggctaaaatt tacggctatg
     4741 aggccggact gcgcataacg gccatcgaaa aaaccagcga cgcttacaaa cagggtttgc
     4801 agcaaaacga tgttattacc aagatcaacg gtatttcggt aacctcctat gaccagtttt
     4861 atcaggagga aagcaaatat aaggcgggga actccgtaac actcaccata ttccgttatt
     4921 ccagcggtca atccaaaaac tttacagtaa aagtgaagct gcttgaagac aaagggacta
     4981 cgtcaaccac gaccacttca agttccagct caagcagcga ttccagcagt ggaagttaca
     5041 cggatccgtt ctcatatttt aatcagggaa acaactaatt catttacact cctataaaat
     5101 cgatccccgt cgaggacggg gatcgatttg tttgattcag accgatttcg ggtgaaaagc
     5161 gccgcgttat gcggcgcttt tttcataggc aatcggcaga cttcattcgt gttgcgcatg
     5221 gcccgtgtat tggtataaaa caccgttaaa cggttatacc aatagaaaac atcgtttcaa
     5281 tggaaaaggt ttgcttcaat tccacccgct gtaaggagaa ttttacggtg atttcccggt
     5341 cgctgccgga agctgaatct gtttcacgaa tgaagataaa ggacagaagg tgggagtttg
     5401 tttgataaaa taaaagcaag aataaatatc tggaaagctc agggtccaag caaggcagta
     5461 ggggacacgg ttcccgatcc ggagcatgtt aaacataaca tggtggacaa tctgatttat
     5521 tttcagggga agtttgccgg ctgtgccgat tttatttgca aagagatgac ggtttgcggt
     5581 aataaagtgg cgctaatgat gattgacaac atggtggata aagttgccct gaccgaaagc
     5641 gtcatgaatc ctctgacccg tgcgcagaaa ccaaacgctc cgatgaatga agaggagacc
     5701 tatctttggg tcagagactg tgtgcttgct tccatcgacc aaaaagaaat tttcactctg
     5761 gaagagtgca ttagtttatt aatgctggga tttgtaatcg ttctgtttgg ggatgaagac
     5821 aaagcgattt ccatcggact gcagggtttt aaataccggg gtattgagga accaaatctt
     5881 gaacaggttc tgcgcgggtc aagggaaggg ttcgttgaac cgctgcgaat taacatgtca
     5941 atggttcgca gaagggttaa aaatcccaac ctgaaatttg aagttgtgac gatcggaaat
     6001 gaaagtaaaa ccgatatttg tctcacttat gtcaagggag ttgcttcgga aagtatcctc
     6061 agcgatatca ggcacaggct gcttagtatc gatatcagca tggttctggc ctccggctat
     6121 attcaggctt attttcaaaa taatccctat tctatttttg cgacggtcgg aacaacggaa
     6181 aggcccgata cgctttgcgg taaactgaat gaaggaagag tcggcattat tgttgacggt
     6241 acgccgctgg ctttgtatac gccgtttctg tttgtagaaa attttcagaa tatggatgat
     6301 tacgcggtgg gcccctatta cgccgctttt accaggctgc tgaaattcat ggcatttttt
     6361 acttctgttt ttgtgccggg cctttatgtc gcaatcggca gtttccatca ggcgctttta
     6421 ccaactcctt tgctggtgac cctggcgaaa gcggaggaga cgaccccgtt tccactggtg
     6481 ctggaagcgc tgctgatgca gtttatttat gaactgattc gggaggcggg gctcaggctg
     6541 ccaaagcaat tcggatttgc catcaacatc gtcggcgcgc tgattgtcgg tgaagcggcg
     6601 gtgactgccg gcctgatcgg agcgcctatg gtcattattg tggcaataac ggcgacaaca
     6661 tcactggtaa cgcctacgct ctatgagccc ggcgtcatcc tgcggtttct gtttattatt
     6721 cttgccggga tgtcgggctt gtttgggatt actctcggca cggcccttat cctgtttcat
     6781 ctgtgttcga tcaagagcta tgagatcccc tttacggcgc ctctggcgcc tttcagcctg
     6841 ttttcaatga gggatctgct gattcgcgct ccgtggaaga tcctttcaag gaggaagatg
     6901 tccgttcaga atatggtcgg atcaaatgta gataaaaatt tggagtgagc gtagcaaatg
     6961 gaaaaaagct cagtaatatc ggtaaaacag ctgttgcttg ttatggttat tttaagaatc
     7021 gcattttcca cgtcgtatat cagcgggctg aacacgggaa actcggttca ggatgtcctc
     7081 gtggcgattc ccgtgttgtt tttgatcagt tttattgttg cgctcccgat tctgattttg
     7141 ttgaaacggc atccgggcaa agccatcatg gaatgttcga ttcaggtcat gggccgcggt
     7201 gtttccgcgg ttatcggctt gatttatatt tcgtttttta tttttcaggc ggcggctgag
     7261 ctggggacct ttcatctggt ttttactaat gctgtgattc cggaatcgca ggagtatgcc
     7321 atatcgatcc ctctgttgat tgtctgcatg tatggcgcgg cgaaagggat cgagtcggta
     7381 tcccgtttcg gaacctttgt ttttgctttg tatgttttga ttctgatctt taatatgata
     7441 tcgctgcttc caagggccca aatgggttat ttgttcccgc tgttttacaa tggaccccgt
     7501 gtttttctcc aatcgctcaa cagtatgttt aattcgagtg tgcagatttt aacacttgcc
     7561 gtctgcgcgt catttttaaa gcccggctca aatgtcagaa aagtattcag ctattataat
     7621 gtttttgctt ctcttcttct atttttgtat acattcctcg ccgtgatggt catggggccc
     7681 tacacgtcca aacaggtttt tcctcttttt acgctttccg aactctcaaa agtcggtgtc
     7741 tttgaacgtc tggactcgct ggaaatggtg ctttggattt ttgataccat attggcggtt
     7801 accgtttata tctttctggc tgcaaatatg ccgaccaaat ttggcctgaa caaatatcgt
     7861 aaagtagtaa ttgtcctgat aggcgccgcc gtttattttc ttgcccctgt aatctcgcat
     7921 aattatttga tgtaccagag ctgtatggga agtctttacg cgacggcgat tgtggtgacg
     7981 ctgatcgtca ttattccgtt aatcatcctg attaccgata tcatcaaaga gaaggtgaaa
     8041 aagaatgaag cggaaaatta gctttttgct gtgctgctgt gccgcggcga tgcttttttc
     8101 cggatgctcg ccctataagg aactcaagga actctcaatt gtgcagggaa tgggagtcga
     8161 ttccggacag aacggcggtt ataatctgac tttccaggtg ctgaaatcca aaacgtccaa
     8221 taattccggc agtcagagtg gagggggcgg aggagaaaat gaagtttatg tgagttcggg
     8281 aaccacgctg tttgacacca ttcgcaatac cacgctccag atgggaagga agctgtattt
     8341 tcccagcact cagatttttg tgatcggcga ggaagaaagc aaaaaaaatc tggtcgagct
     8401 gtttgacttt atgcagcgca accatgatat gagacccacg gtgaaggttt atgtctccag
     8461 ggggaacgcc aacgggattt tgacggcgac caaaaacgga agccagatcc ccgcggcgga
     8521 tattgcagaa atttcaaaat cctattataa ctcgtccaaa accatcgatg tgacattcag
     8581 cgatatctat atgcagcttg ccaatggcat taccgatccg gtgattccgg tgatttccgc
     8641 cgcaaatgac aacacaggca agagcatggt ggagatgcag ggatccgcgg tatttaacaa
     8701 aaacgtcctc gcaggttatc tggatgacag ccagacccgg ggctatctgt ggattatggg
     8761 aaaagcggag agcggaattg ttgttgttta cccctctgct ggaggcaagg tgagcctgga
     8821 agtgaccaaa aataaatcag atgtaaaaat tgcgcaggac aaaaacggtc aggcgattgt
     8881 taagataaca attgacgtca gcagcaatat gggggaagtg caggggactt attcgtcgat
     8941 gggcatggaa tataaacagg aactgatccg tcttcaggct gaagtaataa aaaaagaagc
     9001 ccagtcggcc attgacggcg cgttaagggc ctataattcc gatatatttg gcttcggaat
     9061 gaaaattttt cagtccaagc cggatgaatg gaaaaagctt tcaaaaaact gggatacgga
     9121 tgcgcataaa ataccggttg aaattacggt aaaatccgaa attcggcaaa gagggatcat
     9181 ttcaaagtag aattccgcaa catgtcaaaa gcctgaattt ccatttttaa cggaagttca
     9241 ggcttttgtc ttaattttag taacgattcg gtagaaactg atccataaaa tgtacgctga
     9301 ccgcgagcaa cgtcagggta acgacggctt tctccaaata ccggatttct ttgcggccgg
     9361 tagctctgcg cgataaaatc agaaacaaac ggtagcatgc ttcaaaaatg cagagcaaat
     9421 acaccgcgat tccggcggga ttcatcgccc acgcgttgcg gaactggaaa tggctcaggt
     9481 agatgaagca ccgggtcatg ccgcagaccg ggcattgata gcctgtcgct tcgcgaaaaa
     9541 gacaaaatgt tccgaccggc tgaaagtggc cgttccattc aaatgtggtt ctgacgccat
     9601 cggtatgaag gatgaccgat aatacgagag ggacaagaca aagcagaaac agcgtcaggt
     9661 gtagcggaaa attatctccg gcagctattt ttgaacggaa atcacgagcc atatctgctt
     9721 ttctgcattt gagtttcaac ctgcctgcgg aattcctccc agttaaaacc gccgattgcg
     9781 agactaatca acattttaat aaagatacca accccgacaa agcctaaaac aagaccggcg
     9841 attgccatgc cttttccttt cagcgctccg cctgattttt tgattcggtt attggaaata
     9901 aagccgaaga taacggcgag aatacaggga attatgccaa tacagcaaaa aacggtaaca
     9961 actcctgtga tacccaaaac aagggaagcg atggcaaaag aatcattttt cggggcaagg
    10021 taagggtcct ggtacgagta acggggatca tatcccggag cgccgccctg agggccgggc
    10081 tgcggcggat aaccgggatt ttggggaaca tttgtcgcat tttgcggcgg aacccctaaa
    10141 tccggctgtt ggttttccgg attctgggca ttctggccgg agctctgggc cgaagtgtca
    10201 ggcgtcggat tttcgcttgc ctgatgatcg ttgccaagca gataaccgca gtttctgcag
    10261 ataatatcag catcttcaca ggctgatccg cattttggac atgttttcat tttatattcc
    10321 tcctctaatt gatcaaatgc tgtcaaagaa agcgtgaagg tctgtcatat gccggccgaa
    10381 ctgctgtact gattaaaata attgcccatg ccggagaggc tgatgagact gagagaaatt
    10441 agtaacacga cgatgattcc aatagtcagg gttacgattc caagcacgat cccggtaatc
    10501 gccatcccga aaccctttga agcgccggtg ctgattttgc tttttgccac aaatccaaaa
    10561 ataatcgaga ggattgccgt aataactccg gaaccgtaaa agcaaaacag cggcaggcta
    10621 agaatcccga gtatcatgga agcaatcgcg aggccgctgt aactctgttc cggcgggtat
    10681 tgccggtatg gggtgtagcc gtaatacggc tgcccgggaa aagtctgata tcccgcgtat
    10741 tcgttttgtt ccttctgtgg cgggggaaag ggacagccac agtatttgca gtatgtatca
    10801 ctgtctgctc ccgtttttct gcatacggga cattctttca ttctcatacc ctccgtattt
    10861 tgagtgattt cggcaggccg ccgatttgaa cagtcgattc cgcattaata tcataatatt
    10921 gatccggtgg gattattaca aattaaaatt ccgtggaatt ggttggtgtc tcatccagaa
    10981 tcagtttttc cagctcgctg gtcgtggctg catactgccc aaaacaagag agaatctcct
    11041 ccctggggcc ataaccgtac aaaacgccga tgttgtcgat gccggtttgt ttcgctccct
    11101 cggcgtcaaa cttccggtca ccgaccaaaa cggtcttccg gtttagaggt gcgttcatat
    11161 ctcttagcgc agaacgaatg gaatcggcct tggttgactg ataattcagg cggggagccc
    11221 ctcccacagc ttcaaaataa tccgacagcc catggtattt caaaaccgtt gccgccatgt
    11281 gttctgcctt tgaggtcgca acaccgagtc tgagcccccg gaagctcaaa ttttccagca
    11341 atggtcttac accgtcataa accgttgatt ggtaaagacc ggtttcttca taatccgcgc
    11401 ggaaaagccg gatcgcctgc tccgccgtct cttcatcaag accgcagacg tcggtaaagc
    11461 agtatttgat cggagggccc ataaagagat tgagtttgct ttccggagga atgggaatac
    11521 ccagcccggt cagtgctttt ttgacacatc tcaaaatacc gggctttgta tcaagaaggg
    11581 ttccgtcaac gtcaaagagt acaatttcat aattgctcat tccatgaaac ctccgtaaaa
    11641 tgagggtcag cggattcaag aagtattaca gcatttccaa ttaaaatgta gaattgtctt
    11701 gttccccacc tttggcgggg agagaaaaac acactccatc tcgcaattct aaataattat
    11761 atcattaaat ttattttctg tcttttatta tgatgaaatt ttgcttttaa ttgtaaaaaa
    11821 agggacaaac acagcaattt tgatatttca tgtcaccaat attaaggttt ccaggaaaac
    11881 atccgatatt ctgacctaaa gtattgacaa aacaataagc aaggatataa tatataaggt
    11941 ttgtaaagtg aaatcggggg taactatgca gaaaaaaaat tatgatgttg ttattatcgg
    12001 cggaggaatc ggcggcttga tgtgcgccta tagaattgca caaaacagcc ccggaactac
    12061 cgtcgcactg attgaaaagg gtcaggattt aaaaaagagg tcttgcccga ttgtgacggg
    12121 aaaaagcaaa aagtgtatca actgcgcttc ttgtgcgatt atggaaggac ttggcggcgc
    12181 tggggccttt tcagacggga aatacattat ttcctgtgaa tacggcggat ggctgcctga
    12241 cttgctgccg cacgaaaccg tgatcggtta catcgagcag gccgacagga tattggtttc
    12301 gtttggtgcg acaaccgagc ggttcagccc tgataatgag ctcaaactca aatgtctgcg
    12361 gcatgatctc catatgcagc aggcggttgt gaaacacctc ggaaccgacg ccaactacga
    12421 taccatgctc aaaatggtgg aagaagttgc gaaaagcacc gaaattgtca gcgaaaccga
    12481 agttgttgac gttgacaggc aaagtcataa aattctgctt cgccacaagg gtggggaaga
    12541 ggaaattacg gcggatcgga ttatctttgc tgtaggaagg gcgggcagcc gctttttcag
    12601 ggaatggtgc ggcaaaaacg gcatcggcct gcagaataat caggttgatg tgggcgtaag
    12661 agttgagctg cccgccatta tctgggatga attctccaaa aaaatctacg agcccaaaat
    12721 ctggtatcgg agcaaagaat acggcgacat cagccggatg ttttgcttta atgagcgggg
    12781 aaatgttgta accgaaaaca cgcagggcgt tttgaccgtc aacgggcatg cgtacaaaga
    12841 tcccgccaaa aagacggaaa actccaattt cgcgctgctt tccacaattc ggttcaccag
    12901 cccgttccgg gagcccattg agtacgcgcg gcatgtggca agccttgcca atctgatcag
    12961 cggcgggagt gtccttgtgc agcggctggg tgatctggag ctcggaagaa gaacagatga
    13021 gaaaagactg agagcatgca caacaaggcc gacgttcgac gcggttgccg gtgatttaag
    13081 tctttgcatg ccgaaacgcc agctcgacaa tatcattgaa acgctgcatg cgctcgatca
    13141 ggtcgcgccg ggaaccgcca acagcgatac gctgctgtat ggtgtggaat gcaaatatta
    13201 ttctgcaagg cctgattcgg aggaattcga aatcaaaggc tgtgagcata tttatgcaat
    13261 tggagatggc gccggattta caagatcact ctcacaggcg tcggccaacg gcttgtatgt
    13321 cgccgaccgg atcacaaagg gcaattgaga caaaattacc aaatcatccg ggaaatgtgg
    13381 ccataaaaag ggatataata acaaaaatcg agttatgttt cttctaaaaa atatagtgta
    13441 atctttatat ttcgccgaat taacataata aaatcgtttt gttaattacg ttaaacaaaa
    13501 tccgtgcagt tttacaataa ttgtaaaact gcacggattt ttcatttcaa atttattttc
    13561 ttcaaggata aagtccgatt tttgaccgtc tgatttgaat gaagtttgat attttaaaga
    13621 tgtataaaaa ttcactaata tacgaattca tacaaacccc aaaattgtat tgtcagggcc
    13681 ttgaacgtct tgcggaatat cggcgatttc cggagctctg cggtctgggg tgccaaagat
    13741 attgatcggt ttcttttacc acgacatttc tgagtgctaa tatggaaaac aggtttggaa
    13801 tgcacataaa cgcgttggcc atatcggaaa agttccagac tgctcctatt gaaaaaacgg
    13861 agccgataaa aatcagcagg acgtaaagga actgataggg ccttaccgca cgcgggccca
    13921 gcaggtattc caccgctttc tgggcataaa atgaccagcc gatacaggtt gcaaaagtaa
    13981 aggttaacag ggcgagagac agcatgaccg gcccgattac gggaatcgct gcgaaaactt
    14041 caccagtcag tgtgccgccg tcggaagtct gagtccataa tccggagttg acgatcatga
    14101 ttcccgtgac agccgcgagg atcaccgtat cccaaaaggt tccggtataa gagaccaacg
    14161 cctgacggac agggttgccc gttctggccg ccgctgcgac aatcggttcg gaaccgagcc
    14221 cggactcgtt ggagaaaagc ccgcgtgaga ttccgtagcg gcaggcttct ttggccgcgg
    14281 cgcctgaaaa accgcccagc gccgcctgac cggaaaaagc ggatgttaca atgagctgta
    14341 tggtgtgggg aatcgaatac cattgcaatc caagaatcag aagattgctg agaatataga
    14401 aaagccccat aaccgggatc aaataactgc atactttgga aatgcttttg attccgccga
    14461 taatgacgag agacgtaagc accgccacgg tcacgccggt gacccagggc ggaatcgcgc
    14521 aggttttgag catcatacct gagatggaat tggcctgaac catcgctccg attccaaacg
    14581 ccgcaatcgc ggtaaaaaat gcgaacagca tccccatcca ttttttatga aggccgcgtt
    14641 ccataacgta cattgggccg ccggccatgg aaccgtccgg cattttgacc ctgtatttga
    14701 ctgaaagcag cgattcggca tatttggtcg acataccgaa aatgccggaa atccacatcc
    14761 agaaaacagc gccgggcccg ccggatacga tggccgtggc cacaccgacg atattgcccg
    14821 tcccgatcgt tgccgcgagg gcggtggtca gagaggaaaa ggggctgacg tcccccacgc
    14881 cgtgttcctc tttggaaacc gacagccgga tcgctttgaa aacatgcagc tgtattcctc
    14941 tgagtttgaa tgtcaggtag atatgggttc cgaacagtgc cgcaatcatc ggccagctcc
    15001 aaaggattcc ggcaaaatcc gaaacggctt tttccaaatt ctgcaacggt gttcctccct
    15061 atttctattt ccttattaaa tatatatcct gcgcccgctg attatgatag gacgaccaaa
    15121 gcctgttttt cgttttggag ttccgcaggc cgtatatttc cgattaacgg gaacataatt
    15181 caaaagagat cagaaatggg aggaacgaaa atgaaagaaa ctttgccgat tgagcaggtt
    15241 cacgcgcggg aaattattga ttccagagga aatcccaccg tggaagcgga agtaatgctg
    15301 gagggcggag cagtcgggtg cgccgcggct ccctcgggag cgtccaccgg agtgtttgag
    15361 gcgcatgagc tgcgcgactt ggatcagtcg cgttttttgg ggcgcggcgt gcgtaaggca
    15421 gttgacagcg tgaatggtga aattgccggg gcggtcaggg gattggattc cctctcgcag
    15481 tcgcagcttg acaacgtgat gattcagctt gacggaaccc cgaataagtc aaggcttggc
    15541 gccaatgcga ttcttgccgt ttcgcttgcc ggctgcaaag ccgccgcaag cgcgctgggc
    15601 cttccgtttt accagatgct cggcggggtt gacgcccgca cgctgccggt tccgatgatg
    15661 aatattctca acggcggcgc gcatgcttcc aacaatgtcg acatccagga gtttatgatc
    15721 atgcctgtgg gcgccccttg cttttccgag ggacttcgct ggtgcgccga ggtgtttcat
    15781 catcttaaaa aagtcctttc tgataaagga atggctacct cggtgggaga cgagggcggc
    15841 ttcgccccga accttggcga cgacgaggat gcgcttaatg tcattctcga agcggtggaa
    15901 aaagcgggat atcaagccgg aaatcaggtt aagattgcga ttgacgcggc atcctccgaa
    15961 tggtatcaga aagacggaac ctatttgctg ccgaaaaaga ataaaaagct gcagcggggc
    16021 gaactgatcg aatactggaa acggctcgct gaaaactatc ctattatatc actggaagac
    16081 ggtgtggctg aggaagactg ggacggctgg cgcgatctta ccaccgcgct tggagaaaaa
    16141 attcagctgg ttggcgacga cctgtttgtt accaatactc aaaggctgca aaaaggcatc
    16201 tccatgaaag tcgccaattc tattctggtt aaactcaacc aaatcggaac cctgaccgag
    16261 acgatggagg cggttcgaac cgctcagcgg gcgggataca cgacggtcat ttcccatcgc
    16321 tcgggcgaga cggaagacac aacgatcgcc gatcttgccg tcgcggtcaa cgccggacag
    16381 atcaaaaccg gagcgccgtc gagaagcgat cgggtcgcga aatacaaccg tcttctccgg
    16441 atcgaggaag aattggggtg cgcagcgatt tatccgggca aatgcgcctt ctataatcta
    16501 ccctccgctt aagtctggcg acccggcccg cccaatcatc agtgcagcca ggccgtatat
    16561 gacaaaagcc gctccaaacg gagcggcttt ttaaaagcgg aaagtatgta agcttttaga
    16621 gctggttgat catacccatc acaacacggt ttgcctgcac cgacgctttt ttctcaaatt
    16681 cattgtaaga catctgcgcg ccctcgtcgg ccaaatcgga aattgagcgg ataatcagga
    16741 acggaacatt gttcacgcat gcagcgtgcg cgattgccgc cgcttccatt tcgcagcaca
    16801 tcccgcccat ttcaagaatc ctttttttat cctcaccggc ctccacaaaa cggtctccgg
    16861 tcgcgatgcg gccccggcag aagtaatcga cttcgcgggt ccctgcgcag actttctcag
    16921 ccaattcgac catttgggga ttacattcga aaacggaggt gaaaggataa tacttgtcca
    16981 aaagatccgg cggctgaaaa tcgtggtata ccacatctgt gccgatcacg acatcaagcg
    17041 ttttcaggtg tttgccgata ccgcccgcga tgcccatgtt gataatataa tccacgccga
    17101 ctaaatcaat gagcctttgt gtgctgatcg ccgcgttgac cttgccgatt ccgcagcaga
    17161 ccagataaat ttcattttcg gcggcgttgc ctttatagag ctggtaaaag ccttttttag
    17221 gaacctcttt ggcgttcagg tctttgataa aaccttccat ttcaacaggc atagctgaaa
    17281 taatgccgat tcgcgccatt cctgatcact cctcagtctt aaaatacgga tttgacctac
    17341 ctattataac atcagcctca ccggttttca aacggagccg gttattttta ctaagactcc
    17401 atcaatatta aatttggtaa atttatcttg aaaaacgaga atcttgttgt taatatagaa
    17461 acaagttaaa aattgttgat ttaaggaaga cgtgcaatga atgaatcgaa aatcaatgga
    17521 ttatcaaaat atcaggatac atataaaaag ttcgctctat gcctgatact tcttttcact
    17581 ttttttctga gttattataa tttcggaaaa gatggatttg gaaacctgta ttatacggct
    17641 gcgataaaaa gcatgatgtc aagctggcac aatttttttt acatttccta cgatcctggg
    17701 ggatttgtca gtgtggacaa gcctccgatt gcgctgtgga ttcaggttgc atgtgctaag
    17761 atattcggta tccatagctg gagccttatg cttcccgagg ctttggctgc ggtaggaagc
    17821 gtagctgtgc tgtatcatct gacaaaacgg attttcggat atcttgcagg cattattgcc
    17881 tcttttgcac tcgcaatcac accgattttt atcgctgttt cacgctcaaa caatccggat
    17941 gcaatattgg tttttctgct tgttcttgcc gcatggcagc tcattgaagc ggcgaacaag
    18001 ggaaatttaa agcatttgat tctggctgcg gtcctgattg ggcttggctt taataccaag
    18061 atgctggtcg cttttttggt agttccaagc tttctattgg tttacctttt tgcgagctat
    18121 atcaaattac tcaaaaagtg catacacctt gtgattttcg gcatggtgct gcttgcagtt
    18181 tccttttctt gggttatagc agttgattta acccctgttt cacaacggcc ttatgtggga
    18241 agcagcagta cgaattcaga aatgaatctt gtgtttggat ataatggatt cagtcgggta
    18301 acacaataca attttacgtc tgaaaacggg ctgaatgcag gtaatcatgc agcagaaagc
    18361 agttcttcta tgaagtctcc tggctccatg ggaaatttca aactattcaa tatctatttc
    18421 gggcagcaaa tcaactggtt tgcagcactt gcggcatttg gtgttttaag tgcccttttt
    18481 tactgcaaat ctttaagcgg agaagagaaa aatcaaaagt tacgttatct cctgttatgg
    18541 ggcagctggg ttttcatcat gtatctcttt ttcagtgttt atcaaaatat gactcatcgg
    18601 tattatactg cggtgctggc tccgggtctg gcttcactgg ttggtatagg catatcttgt
    18661 tttcgtaaga tgcgccgcca tccattcatg gctttggcat ttccggttac tttaattata
    18721 agcgccgggt atcagctttt tattgtttca gtttatcatt ctcttgcgag agttttaacg
    18781 ccgattttaa ttgcagggat cttttttgcc gttatactga ttggcattgc tttttttaag
    18841 aaaaagaatt taggcgcaaa aaaatgttcc cgtattttaa gtatatgctg cctggcaatt
    18901 ctttttctgg cccctgccta ttggtcattt acgacggtaa tcgggcgggt gccccaagcc
    18961 gatccatatg ccggaccggg acttttaacg aaggctcttc ctgattttca atcaattgtt
    19021 aaaaagctga gtttaaatac atctgatctg aaaattccgg attattacaa acaaacagca
    19081 aactatttag aagcgtacca ggaatcggca gaatatttgg cggctgtgcc aagtgcctca
    19141 ttaatgtcgg aatatctgat tcttttaacc ggcaagccgg ttatgaatat tggcggttat
    19201 tccggcacaa atgcgatttt aactctggat caattcaaac aaaaggtttc atccggtaaa
    19261 ttaaaatatt ttattaccga ttctcatgca gattcaagtg taaatacaga tatcatcaac
    19321 tgggttgttg aaaacgggaa aaaagtaaat accaatatta cggacaaaac aaagacatca
    19381 tccaattatt caatctatga tatatctgta atttattcat aaacgtgttg gaatggaaag
    19441 aggcggttca ttgatgatta atggcatgaa acaggatatt cacaatagct tgattgaaca
    19501 agttagaatt tctttattaa aaataattaa atcgtcggta tcggatttta cgatagaaag
    19561 caatgacgca ggatcagatg tattgattat ggcaattcac ggtggaaaaa tagaacccgg
    19621 aacgactgaa ctggccagaa atttatcacg aaaatataat tataactatt actcctttgt
    19681 ggggaaaaaa agcaatcata acgcttcttt acatataaca tctaccgact ttcgagagcc
    19741 aaaggcaatc gaaatgtcgg cgcgatcttc tgcaaccatc tcaatccacg gctgtgccgg
    19801 gtcgaatcaa tttacctata tcggcggaag agatgatgac ttgggaatga aaattaaagc
    19861 ggctttagaa aaaaatgggt ttacggtatt agcggccgcg ccgggactga gcggcaaatc
    19921 cat
//
LOCUS       DATALY010000346         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_679572,
            whole genome shotgun sequence.
ACCESSION   DATALY010000346 DATALY010000000
VERSION     DATALY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679572"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..2290)
                     /locus_tag="VHR42_09545"
     CDS             complement(158..2290)
                     /locus_tag="VHR42_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027447.1"
                     /translation="MLKTHTFKFDLNLQMKPADGILSFDETGQIKPCIGGKNLIGLSA
                     DDMEEATVIAGVGCGLIDARMKDGSERLLCRFTMSAVKAAGEFCKVVNYYIQTKQCAF
                     PDKVENPVCEKCGRPFVEGMNVCLFCYNKMGVLKRALMLMRPFARKIIPAEIFLAISS
                     ILFLFVPLFNRLLIDNYLEPMTGTFQQILLLVSGMFAARALGEVIYILSSRVFNKASI
                     EFSNYLRNLAYEKIQKLSLNSLSKRTPGDLIRRIMEDTNNIRNFLSDGGRWAVEQIIM
                     FIVVMIILLLTNVKLTLLAFFPVPIVMFGMSRFWRFINIRYERQWRKNSKCQSILHDI
                     IKGIRTVKSFGKEENEIRKFSGATRELAEVSSDNEALWARLFPLMTFFTGIGEFLVLY
                     FGGRNILGNALSLGVLYQFTMYISYIYEPLRWIVSFPRWLADAMTSMVKVFEILDEEP
                     DIAAISHPKNVPVSGGVSFEGVSFGYKSYEPVLKDVSVDIRPGEMIGIVGHSGSGKST
                     MINLIMRLYDPSQGKICISGTDLRDISPAYLHENIGVVFQESFLFAGTIYDNIAYAKP
                     GATAAEVFAAAKAANAHDFIMKTPDGYHTIIGESGLSLSGGERQRISIARAIIKNPDI
                     LILDEATSSLDVETESIIQDSLNRLVKGRTTIAIAHRLSTLRSADRLIVLDKGKVAET
                     GTHIELLKQKGIYYKLVMAQRQTSRIAK"
     gene            complement(2454..>2890)
                     /locus_tag="VHR42_09550"
     CDS             complement(2454..>2890)
                     /locus_tag="VHR42_09550"
                     /inference="COORDINATES: protein motif:HMM:NF020483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HEX3027448.1"
                     /translation="NLSDAIDIGLLDLTKAEFFVTPGGFTGLKYGNKEYSRITLRRAL
                     PIGHPMEYISVADKDNKEIGMIRSVLDLSEQQRRIVVNELENRYYCPVVQEVKSVKDK
                     LGYVYFELVLGHDDETYEKNCAIKDVNKNIRVLDNDRLIILM"
BASE COUNT          770 a          663 c          651 g          806 t
ORIGIN      
        1 tccatagatg gcttggtcga attttcttaa tgatcactat tttagttttt tgtggcataa
       61 aatactccct tcatgatgaa cagtgtttgt tatttcactg tcgctcataa agggagtata
      121 tcaataatcg gtgttttttt acgcatacag cgtggggtta tttggcgatc cgactggttt
      181 ggcgctgcgc cataaccagt ttgtaataaa ttcctttttg cttcagaagc tcaatatgag
      241 ttcccgtttc cgctacttta cctttatcaa gtacaattag cctgtcggcg ctgcgcaggg
      301 tcgacagacg gtgcgcaatg gcaatggtcg tccttccttt tacaagacgg ttcaggctgt
      361 cctgaataat cgattcggtt tcaacatcga gggaactggt cgcttcgtca aggatcagaa
      421 tgtccggatt tttgataatg gcacgcgcaa tggatattcg ctgcctttca ccgccggaca
      481 gcgacagacc gctctcacca ataatcgtat gatagccgtc aggggttttc ataataaaat
      541 catgagcatt ggcagctttg gcggcggcaa atacttcggc ggcggtagct cccggcttag
      601 cataggcaat attgtcgtaa atcgtgcctg cgaaaaggaa ggattcctga aagacaacgc
      661 ctatattttc atgcagataa gcgggagata tatcgcgcaa atcggtgccg ctgatgcaaa
      721 tttttccttg gctggggtcg taaaggcgca taatcaggtt gatcatcgtt gactttcctg
      781 agccggaatg gccgacgatt ccaatcattt cgcccggccg gatgtccaca ctgacatcct
      841 tcagcaccgg ttcataggat ttatagccga atgaaacgcc ttcaaatgaa acgccgccgg
      901 aaacaggtac gtttttgggg tgggagatgg cagcaatatc cggctcttca tccagtattt
      961 cgaacacctt caccatactg gtcatggcat cggccagcca gcgcgggaaa gagacaatcc
     1021 agcgaagcgg ttcatatata taggagatat acatagtaaa ctggtacaga acacccaaac
     1081 tgagtgcgtt gcctaagata ttgcgcccgc cgaaatagag gacaaggaat tcaccaatac
     1141 ctgtaaaaaa tgtcataagc ggaaagagac gggcccaaag agcttcatta tcagaagaga
     1201 cctcagccaa ctcgcgggtt gcgccggaaa acttacggat ttcattttcc tctttaccaa
     1261 aggacttcac tgtccgtatt ccttttatga tgtcgtgcaa tatcgattgg catttggagt
     1321 tttttctcca ctgtctttca tagcggatgt tgataaatct ccaaaagcgg gacatgccga
     1381 acataacaat cggaacagga aaaaaagcaa gcagagtgag cttgacattg gtcaacaaga
     1441 gaataatcat gacgactata aacatgataa tctgttcaac cgcccaccgt ccgccgtctg
     1501 ataagaaatt ccggatattg ttggtatctt ccataattcg ccgaatcaaa tcgcccgggg
     1561 tacgctttga cagtgaattc agagatagtt tttgaatttt ctcgtaggcc agattcctca
     1621 aataattgga aaactcgata ctcgccttgt taaagacgcg ggagcttagt atgtagataa
     1681 cctcgcccaa agccctcgct gcaaacattc cggacaccag caggagtatc tgctgaaaag
     1741 tgcccgtcat cggttccaga taattatcga tcagaagacg attgaaaagc ggaacaaaca
     1801 gaaagagtat ggaggatatt gctaaaaata tttccgcggg tatgattttt cgggcgaacg
     1861 gacgcatcag catcagggcg cgtttgagaa cgcccatttt gttatagcag aacagacaga
     1921 cgttcattcc ttcgacaaaa ggccgcccgc atttttcaca gactggattt tccactttat
     1981 ccggaaaggc gcattgtttt gtttggatat agtaattaac gaccttgcag aattcgcctg
     2041 ccgcttttac cgccgacatc gtaaaacggc agagcagacg ttcactgccg tctttcattc
     2101 tggcatcaat cagtccacag cccactcccg caattacagt tgcctcttcc atatcgtctg
     2161 ctgacagtcc aataaggttt ttaccgccga tacagggttt gatttgtcct gtttcatcaa
     2221 agctgagtat accgtcggcg ggtttcatct gcaggttgag atcaaatttg aatgtatgtg
     2281 ttttcagcat gtcaagccgc catttctcca gcgatggtat agtacgcctt atcattatcc
     2341 gctggaagca tgataagggt aatgatagat agactagaac atataaggct ccagccgcct
     2401 aaggctcttt ttgtccaggc cggaaagaga attgatcata taccggtttc cgtctacatc
     2461 aaaatgatga gcctgtcatt atcaagaacg cgaatatttt tatttacgtc ttttatagcg
     2521 cagttctttt cataggtttc atcgtcgtgg ccaagtacta gttcaaaata gacatatccc
     2581 aatttatctt tgactgattt tacttcctgt acaacggggc agtaatagcg attttctagt
     2641 tcgttcacga ctatacggcg ctgctgttcg ctcaggtcaa gaaccgaacg gatcattccg
     2701 atttctttgt tgtccttgtc cgcaaccgag atatactcca tcggatgacc gatgggtagc
     2761 gcccgtctta atgtaatgcg gctgtactct ttgtttccgt attttaaccc tgtaaaacct
     2821 cccggagtga caaagaattc cgcttttgtc aggtctaata atccgatgtc aatggcatcg
     2881 gataaatttg
//
LOCUS       DATALY010000347         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_419095,
            whole genome shotgun sequence.
ACCESSION   DATALY010000347 DATALY010000000
VERSION     DATALY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419095"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VHR42_09555"
     CDS             <1..569
                     /locus_tag="VHR42_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX3027449.1"
                     /translation="ICGVWAEGGWLRFETIAGREPTGICGTGLIDTAATLLRLGMIDR
                     SGRIDPDRTSAVGETSALVIDGKSGIVLTQKDIREIQLAKAAVAAGIETLLDSAGIQP
                     EEIEKVYLSGGFGSRMHPDEAKEIGLFQKNLKRITACGNTSLTGAVLLLSDDRYRENI
                     REISHLTGYLELSDSDFFNERFIENISF"
     gene            complement(645..827)
                     /locus_tag="VHR42_09560"
     CDS             complement(645..827)
                     /locus_tag="VHR42_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027450.1"
                     /translation="MNKAIGTLTKGVAAGVVIGTAVGMIGNPMNIRKRNRMKKNATKA
                     LHAVGEIVQNAQYMFK"
     gene            complement(983..>1772)
                     /locus_tag="VHR42_09565"
     CDS             complement(983..>1772)
                     /locus_tag="VHR42_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX3027451.1"
                     /translation="DATSIAAKLKANGITPAARICCFRDNATPQVLTGAAVKYSGNHS
                     YMWYDANKKFWLNPYSSEAQQYLISLAGEAVSMGYTQIYLDGLTFPDNGRPSVNAWFG
                     SDTTATKEETLKSFVAAAKQAVNTAGGKVNVMMPSAASMGQGETITGQDQSIYSYDAD
                     GFCPNLCPTLLSALSKIGDQSVTNAEKNPGDTVTAAATYLAGESTGSQLSGTVPFIQA
                     YSGAKTYGATEIFAQISALKSKGITSYILYNPDGQYDLSSLSVK"
BASE COUNT          423 a          465 c          424 g          460 t
ORIGIN      
        1 caatctgcgg cgtatgggcg gagggcggct ggcttcgctt tgaaacgatt gcaggcagag
       61 aaccgaccgg aatatgtgga accggcctta tcgacacggc agccacactg cttcgtcttg
      121 gaatgatcga ccgttcggga cgaatcgacc ccgatcgcac ttcggctgtc ggggaaactt
      181 ccgctttggt cattgacggg aaatccggca tcgtgcttac acaaaaagat atccgagaaa
      241 tccagctggc caaagctgcg gtcgcggcgg gaatcgagac tctgcttgac tccgccggga
      301 ttcagccgga agaaattgaa aaagtttacc tgtccggcgg cttcggttca cgcatgcatc
      361 ccgatgaagc caaagaaatc gggctgtttc aaaaaaatct gaaacggatc accgcatgcg
      421 ggaacacatc tcttaccgga gccgttctgc tgctttccga cgaccggtac agagaaaaca
      481 tacgggaaat ctcccatctt accggttatc tggagctttc cgacagcgat tttttcaatg
      541 aacggtttat cgaaaacata tccttttaaa aaaagaggct gcaacggctc cagccgctac
      601 agcctcttat aaagtgtttg catcagcgcc gttatgggca ctatttattt aaacatatac
      661 tgggcattct gaacgatttc gccaacggca tgaagcgcct ttgtggcatt ctttttcatc
      721 cggttccgtt tacggatatt catcggattg ccaatcatgc caaccgccgt tccaatcacc
      781 acaccggcgg caacaccttt ggtcagcgta ccaattgctt tgttcataac cattcctcca
      841 ttgaaaagat ttcaatggta ttttcccctg tttaccgtta aaaaacacta ataattattg
      901 ccaaaccaaa attaattttt aacagcaaat tttaaactgc tgaattgcaa acctcccccg
      961 tcgcaaacgg aggaggtttg ttttacttta cgctcaaact gctcaggtcg tactgtccat
     1021 ccgggttata gagtatatag ctcgtgatgc cttttgattt taatgccgaa atctgtgcga
     1081 atatttcggt cgcgccatag gtttttgcgc ctgaataagc ctgaataaac ggtactgttc
     1141 cgctaagctg acttccggtg gattcccctg ccagataggt tgcggcggca gtgaccgtgt
     1201 caccgggatt tttctccgca ttcgtgacac tctgatcgcc gatcttactc aatgcgctta
     1261 aaagcgtcgg gcaaagattg ggacagaagc catcggcgtc ataactgtaa atgctttgat
     1321 cctggccggt aattgtctcg ccctgcccca tagaagcggc cgagggcatc atgacgttca
     1381 ctttgccgcc tgcggtattc accgcctgct tagcagccgc cacaaagctt ttcagcgttt
     1441 cttccttggt cgccgtcgta tcgctgccga accaagcgtt aacgcttggc ctgccgttat
     1501 cggggaatgt caggccatcc agataaatct gagtgtatcc catggaaacc gcttccccgg
     1561 caagactgat aagatattgc tgtgcctcgc tggaataagg attaagccag aattttttat
     1621 tggcgtcgta ccacatataa ctgtgatttc cggaatattt taccgcggct cccgtgagaa
     1681 cctggggagt tgcgttatcg cggaagcagc agatcctcgc cgccggggta atgccatttg
     1741 ccttcagctt cgccgcgatg gacgtcgcgt ca
//
LOCUS       DATALY010000348         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_849134,
            whole genome shotgun sequence.
ACCESSION   DATALY010000348 DATALY010000000
VERSION     DATALY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849134"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VHR42_09570"
     CDS             complement(<1..233)
                     /locus_tag="VHR42_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3027452.1"
                     /translation="MPETSLAEFTETTEPFEDSMRGKYLSFILSDEVYAIEIKNVKEI
                     IGIQPITQIPELPGYMLGIINLRGKIIPIMNVRL"
     gene            629..1588
                     /locus_tag="VHR42_09575"
     CDS             629..1588
                     /locus_tag="VHR42_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3027453.1"
                     /translation="MKFLKIGNQSDGLEVSKLVMGTDKIVQKLSSDSEFFYLLDLYTN
                     AGGNCIDTARIYCDGLSEEAVGRWLKGNGVRNRVVLSSKGCFPDSKNRMAPRLSKQDM
                     EQDIDASLKALKTDCIDLYWLHRDDPGRPAEQIIEDLNLFVRSGKIRMIGCSNWKTER
                     IQRAVRYADSAGLSGFCASQIQWSLASTREEIYQDYSVSIMDEHEYEWYLDRNMPVFA
                     YASQAQGFFAKMMTGGLSSLSEKVRARFGSPENMERLKKAADLSSQMGVSLSAAALSY
                     ITCNRLPAAAIIGSKTPEHLRESLEAADLDISPEEADALYRVN"
     gene            complement(1729..3057)
                     /locus_tag="VHR42_09580"
     CDS             complement(1729..3057)
                     /locus_tag="VHR42_09580"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEX3027454.1"
                     /translation="MKTFKFPDTFLFGSATAATQIEGGDKNNNWYEWCKKGKIKDCST
                     TLRADDHWNRYREDVELLSRLNQKVYRMGLEWSRIEPEKGRFDHSAILHYRDEITLLR
                     KKGIKPLVTLHHFTHPLWLSREGDFENPKVVEYFKKYVTYVVENIGDLVEEYITVNEP
                     NVYIANGFFDGSWPPGKKSLRLAFKVLGNMAACHIEGYKLIHQIRKEKGYPGKTMVGV
                     ANHMRNFAPHSRHNPFDVLLTRLVEYLFQDSMIQLMTRGHLGFPASLFVRRCPKGEYC
                     DFIGINYYTRSTVHFIGMKDEVPAGSPKNDLGWEIYPKGLVELCEESYRKYRLPVWIT
                     ENGICDNNDSKRVRFIYDQLAELSQAIENGIPIQRYYHWSLMDNFEWLEGEAARFGLI
                     HVDYDTQKRTIKRSGQFYGELIKNRGVSRELLQKYGLLKTAHRKPAKSHS"
     gene            3255..4097
                     /locus_tag="VHR42_09585"
     CDS             3255..4097
                     /locus_tag="VHR42_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3027455.1"
                     /translation="MSLFVCPCCRGGLAKTKNGLVCAAGHNYDLASEGYVNLLPANRK
                     NTKAPGDNSAMVEARRAFLNRGYYGMLKNTLARMVLEKTAGLCHVPVVADAGCGEGYY
                     TLGVEEAFDSCGRPVSTAGFDISKPAVRQAAKLASKEGRKTEFAVAGVYDVPLEDECC
                     DVVTNIFAPLCDSEFYRILRSGGWLILIQPGRNHLMGLKEVLYERPYPNKTKAYSLER
                     FENRGIDVIHDFITVEGKEEIRNLFHMTPYYWRTPPEASKKLDALEKLITEIEFEIHL
                     FCKK"
     gene            complement(4296..5045)
                     /locus_tag="VHR42_09590"
     CDS             complement(4296..5045)
                     /locus_tag="VHR42_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX3027456.1"
                     /translation="MLTLRICHLYPDLLNLYGDRGNVLTLSKRCEWRGIGSSITPISI
                     GDPFNSEDYDLVFIGGGQDYEQEILQPDLLGQKAQELKNYISGDGVVLAICGGYQLLG
                     NYYKAQNGKEIRFLEALDLWTVAGETRMIGNIAFKSSLLPSEDSQNIIVGFENHSGKT
                     YLGPGVQPMGEVVKGYGNNGETPFEGAVYQNTFCTYSHGSLLPKNPALSDFLIDRALR
                     KKYSDFSGLTLLDDQAEKLAHDTVLNRIVHS"
BASE COUNT         1366 a         1163 c         1268 g         1408 t
ORIGIN      
        1 agtctgacat tcattatcgg aatgatcttg cctctgagat tgataattcc aagcatatat
       61 ccgggaagct cagggatttg ggtgatgggc tggataccaa tgatttcttt cacatttttg
      121 atttcaatgg catagacttc atcagacaaa atgaaggaca ggtatttgcc gcgcatagag
      181 tcttcaaaag gttctgttgt ttccgtaaac tctgccaaac tcgtttccgg catccatgaa
      241 tcagctcctt ttcatagaat gatgtgaggt tgtcttatta tgtcgaataa tgtctttgat
      301 ttcacttaga ttttagcata cataagtcgg ataatcaagt gatttcatgc cttttacgga
      361 tgaaacagga aaaatatatg tgataagtag acaaaaaata tcaaaaacat taaatacttt
      421 agttaaaaat atccaattat tatgaactga tggtgaatga atattttccc aaacggccat
      481 gtgctataat aaaatataag taaaatggag gtgtcggtca taaaaattgc ggcgtttaac
      541 ggaagcccaa gaaaaatcag aaattgaggc ggaaatcgtt cagccggatg ggaaaaccct
      601 cgccgggcct gccagggaag gagctaaaat gaaattttta aaaataggga atcagtctga
      661 cggacttgag gtttcgaagc ttgtaatggg aacggataag atcgtccaaa aattatcttc
      721 cgacagcgag ttcttttatc tgctggatct ttacaccaac gcaggcggaa attgtattga
      781 taccgcgaga atttattgcg acgggctgag tgaagaagcg gtgggaaggt ggctaaaggg
      841 aaacggcgtc cggaatcggg tcgtgctttc ctccaaaggc tgttttcccg attcgaaaaa
      901 caggatggct ccaagacttt caaaacagga tatggaacag gatatcgacg ccagcctaaa
      961 ggcgctgaaa accgactgta ttgatcttta ctggctgcac agggatgacc cgggccggcc
     1021 ggcggaacag attattgagg atctgaattt attcgtacga tcgggaaaaa tcaggatgat
     1081 cggatgctcc aattggaaaa ccgaacggat tcagagagcc gtccggtatg ccgactcggc
     1141 gggactttcc ggattctgcg cgagccagat tcagtggagc ctcgcatcca cccgggagga
     1201 gatttatcag gattattccg tttcgatcat ggatgaacac gaatacgagt ggtatcttga
     1261 ccgaaatatg ccggtttttg cctatgcctc ccaggcgcag ggcttttttg caaaaatgat
     1321 gacgggcggg ctttcttcgt tgagtgaaaa ggttcgtgcc cgatttggaa gccctgagaa
     1381 catggaacgc cttaaaaagg ccgccgacct ttcttctcag atgggggttt cactttccgc
     1441 cgcggcgctg agttatatca cctgcaaccg tcttccggca gccgcgataa tcggctcaaa
     1501 aacgcctgag catctcaggg aaagtcttga agcggcggat cttgacattt ctcccgagga
     1561 ggcggatgca ctttaccgtg taaattagct tttaagcgga aacgatgatg gaatgtgaaa
     1621 aacggtcttt gcaattttgc aaagaccgtt tcatgataaa gtcaaacgca attttggatg
     1681 aatggccggc gtgcacgggg aaaaacgcgg gggcgccggc tgagacgcct aagaatggga
     1741 tttcgccggt ttgcgatgcg cggtttttaa aaggccgtat ttttgaagca gttctctgga
     1801 tacgccccgg tttttaatca gttccccata aaactgaccg cttcttttga tcgtcctctt
     1861 ctgggtgtca taatcgacat gaataagccc gaagcgggcc gcttcgcctt ccagccattc
     1921 gaaattatcc attagcgacc agtgatagta acgctggatc ggaattccgt tttcaattgc
     1981 ctgtgagagt tccgccagct ggtcatagat aaaacggact cgtttgctgt cattgttgtc
     2041 gcagattcca ttttccgtaa tccaaaccgg cagacggtat ttccggtaag actcctcgca
     2101 tagctcaacc agaccctttg ggtaaatctc ccatccaaga tcgtttttcg gggagccggc
     2161 gggaacctcg tccttcattc caataaaatg aacggtgctc cgggtgtaat aattgatgcc
     2221 gataaaatcg caatattctc cttttgggca gcgacggaca aagagggagg ccggaaaacc
     2281 aagatgtccc cgggtcatca gctgaatcat gctgtcctga aacagatact caaccagtcg
     2341 ggtcagcagt acgtcaaacg gattatgccg gctgtgggga gcaaaatttc tcatatggtt
     2401 tgcgaccccc accatggttt tccccggata acccttttcc ttgcgaattt gatgaatcag
     2461 cttgtagccc tcaatgtggc aggccgccat atttccgagc actttaaagg caagccgcag
     2521 gcttttttta cccggaggcc agcttccgtc aaaaaagccg ttcgcgatat aaacgttggg
     2581 ctcgttgacc gtaatatatt cttccaccag atcgccgatg ttttccacga catatgtaac
     2641 atatttctta aaatattcca caacttttgg attttcaaaa tcgccttcac ggctcagcca
     2701 gagaggatgg gtaaaatggt ggagcgtaac cagaggcttt atgcctttct tccgtaaaag
     2761 ggtgatttcg tcgcgataat gaaggatggc tgaatggtca aaccggcctt tttccggctc
     2821 aatgcggctc cattccagac ccatccggta aaccttctgg ttcaggcggc tgagaagttc
     2881 gacgtcttcc cggtaacggt tccagtgatc gtcggctctc agtgttgtgg agcaatcttt
     2941 gatctttccc tttttacacc attcatacca gttattgttt ttgtcgccgc cttcaatctg
     3001 cgtggccgcg gtggcgctgc caaacaagaa agtatccgga aatttaaaag ttttcatgat
     3061 ccaaattcat cctttattgc atatgcgtcg aatcgattcc gggttttgat aattatttca
     3121 ttataatcat ttaatggaaa ttaatcaaca ttaaatttca cttgaatcag ccgcccggcc
     3181 ggcctataat agaaatgctc caaaaaccgg acaaatcaga aaaacgggcc ggatgtcaga
     3241 aatggatggt aattatgagt ttgtttgtct gtccctgctg ccggggaggc ctggccaaaa
     3301 cgaaaaacgg gcttgtctgt gctgccggcc acaattatga tcttgcgtct gagggttatg
     3361 tcaatttact gccggcgaac cgtaaaaaca caaaagcgcc cggtgataac agcgctatgg
     3421 tcgaagcgcg gagggcgttt cttaacaggg gatattacgg aatgttaaaa aatacacttg
     3481 cccggatggt tttggaaaaa acggctggat tgtgccatgt tcccgtggtg gcggatgcag
     3541 gctgcgggga agggtattac acactgggtg tcgaagaggc gtttgactct tgcggaagac
     3601 cggtctcgac ggcgggcttt gatatttcca aaccggcagt cagacaggcg gctaaattgg
     3661 cgtcaaaaga gggaaggaag actgagtttg ctgtggcggg ggtctatgat gtcccgcttg
     3721 aggacgagtg ctgcgatgtc gttaccaata tattcgcgcc cttatgtgac agtgaatttt
     3781 accggattct gagaagcggc gggtggctga ttctcattca gcccggccgg aatcacctga
     3841 tggggctcaa agaagtcctt tacgaacgcc cttatcccaa taaaacaaag gcttacagtc
     3901 tggagcgctt tgagaaccgg ggaattgacg tcattcatga ttttattacc gtagagggaa
     3961 aagaggaaat tagaaactta ttccatatga ccccttatta ctggagaact ccgcctgaag
     4021 cgtcaaaaaa gctggacgct cttgagaaac tgattacgga aattgaattt gaaatccatc
     4081 ttttctgcaa aaaataagat aaggcagtct ctgaattctg cccttttttg gcgggaaaaa
     4141 gttaatttta tatccttttg agtcagaata tgttaaaaca ttacctatta tatatcattt
     4201 tatctcccga aaaaaacatc cccgtgattc ggggatggaa agaaaaaagg gctaccggta
     4261 accggccagc cctttcactg tttttaatat tcttttcagg aatgaacgat ccggttcagg
     4321 acggtgtcat gagccagttt ttcagcctga tcatcgagca gcgtcagtcc ggaaaaatcg
     4381 ctgtactttt ttcggagtgc ccggtcaatc agaaagtcgg agagggccgg gttttttggc
     4441 agcaggcttc cgtggctgta agtgcagaaa gtgttttgat atacggcgcc ctcaaaagga
     4501 gtttctccgt tgttgccgta acccttgacc acttcaccca ttggctgtac gcccgggccc
     4561 agataagttt ttccggaatg gttttcaaat ccgacaataa tattttggct gtcttcggat
     4621 ggcagcaaac tgcttttaaa agcgatattt ccgatcatcc tggtttcccc tgctaccgtc
     4681 cataaatcga gcgcttccag aaagcggatt tcttttccgt tttgggcctt ataataattt
     4741 cctaatagct gatagccgcc gcatattgcc aaaacgaccc cgtcaccgga aatataattt
     4801 ttcagttcct gcgctttttg tccgagcaga tccggctgga ggatttcctg ctcataatcc
     4861 tgcccgccgc cgatgaacac caggtcatag tcttcactgt taaaagggtc gccgatgctg
     4921 atgggtgtta tacttgaacc gattccgcgc cactcgcatc tttttgagag agtcagaacg
     4981 ttgccccgat cgccataaag attgagtaag tcaggataaa gatggcagat tctcagtgtc
     5041 aacatcattg ccagaactcc tttagcccgt atttttcctt taaaaagcgc ctgatctcca
     5101 tcattgccgt ataagtcgga atgatgtaat gggtagcccc gctgtttgtt gaggcaagac
     5161 ctttctccgt cagcacgctg aaatccttga cgatttcaat cctgt
//
LOCUS       DATALY010000349         7049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_760741,
            whole genome shotgun sequence.
ACCESSION   DATALY010000349 DATALY010000000
VERSION     DATALY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760741"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VHR42_09595"
     CDS             complement(<1..442)
                     /locus_tag="VHR42_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX3027457.1"
                     /translation="MIFGKKKEEDLQEKQNKTVFFKRKNFKYGSTSTIFTIIFIAAVI
                     LLNVVLTAVNDKVTLGVDLTNTKTYNLSTATTKYLKTIKTNTTIYILADKKVLMASSY
                     YAKTIKIIEKFEQTNSNITIKYINLDNNPTFASQYSGESLAIGDV"
     gene            complement(458..1159)
                     /locus_tag="VHR42_09600"
     CDS             complement(458..1159)
                     /locus_tag="VHR42_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3027458.1"
                     /translation="MGAIFRREFKSYFSSAIGYVFLSVFLFFSGYFFINVNIGGNTND
                     MSSFFSSIIFIYVFLIPILTMKLMCEDKKQKTDQILLTSPVSLSGIVIGKFLAALCVF
                     AMALSSVAVFGIILAVMSSVDGWVIVGNIVAMLFVGSALIAIGIFISALTESQMIAAV
                     ASFFIMLMLFMADNFSSSTNAVLSAITGAISITTHYNNFALGIFNLADVVFYCSMAAI
                     FLFLTMRVLEKRRWS"
     gene            complement(1159..2283)
                     /locus_tag="VHR42_09605"
     CDS             complement(1159..2283)
                     /locus_tag="VHR42_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027459.1"
                     /translation="MIEVSHLTKRYGDKTAVDDICFSVKEGEILGFLGPNGAGKSTTM
                     NIITGYLSSNEGSVKVAGIDVLENPSQAKKHIGFLPEQPPLYLDMTVKDYLNFVYELK
                     GTKLKREAHLAEICDLVKITNVYGRLIKNLSKGFRQRVGLAQALVGNPDVLILDEPTV
                     GLDPKQIIEIRSLIKHLSKKHTVILSSHILPEVQSVCERIVIINKGRIVADDTAFNLS
                     TNLSAEHRLVARIAGPEETVRRALSGISGVRLVTPLGIREKDSFDFQIEMEPGKDVRR
                     ALFSVLSDRKWPLLFLDSGEMTLEDIFLKLTSEDVAPAIRKKKKVDYQKDPKYTRMIE
                     RDTPEDDEDNTDEMLNSQAESLLSGQKKQEPETKEKEDKE"
     gene            complement(2441..4237)
                     /gene="aspS"
                     /locus_tag="VHR42_09610"
     CDS             complement(2441..4237)
                     /gene="aspS"
                     /locus_tag="VHR42_09610"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX3027460.1"
                     /translation="MAETMKGLKRTNYCGEPRLEDIEKQITVCGWVQRRRDLGGILFL
                     DLRDRTGILQLAFDEKTPAGVFEKAFTVRGEYVLAAKGVLRKRDSGNSKLPTGEVELA
                     VEELRILSKSETVPFEITEKTDVKEELRLKYRYLDLRRPEMQRNIALRHRIAKTARDY
                     FDRNGFLEIETPVLIKSTPEGARDYLVPSRVHPGSFYALPQSPQLYKQLLMISGFDRY
                     MQIARCFRDEDLRADRQPEFTQIDLEMSFVDSDDVMEVNEGFVHRVFREILGVEIPVP
                     FSRLTYREAMDRFGSDKPDTRFGMEITDLSDLAAGCGFKVFTSAIEGGGSVRAINAKG
                     AFQKLTRKELDKLAVFVKTYRAGGLAFTRLNGESTSSSFAKFLTEDEARAIYDRLDAH
                     DGDVVMIVADKNNEVVLESLGALRLEVAGKLDLIPKDRFEFLWVTEFPLFEYSAEEQR
                     YTAKHHPFTAPMEEDVGRIESDPGSCRAKAYDMVLNGVEVGGGSIRINDTELQKRMFT
                     ALGLSEQTARERFGFLIDAFKYGAPPHGGMAYGLDRLAMLMLGCDSIREVIAFPKVQN
                     ASELMSSSPAEVDQKQIEELHLRLTETDQEKS"
     gene            complement(4270..5577)
                     /gene="hisS"
                     /locus_tag="VHR42_09615"
     CDS             complement(4270..5577)
                     /gene="hisS"
                     /locus_tag="VHR42_09615"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX3027461.1"
                     /translation="MANQLQAPRGTLDVLPDKVYQWQYVESAMRDTAAAFGFREIRFP
                     TFEYTELFSRGVGETTDVVQKEMYTFEDRGGRSVTLRPEGTASTVRLVLQNGLHNEPL
                     PLKLFYLISCFRGEKPQAGRYREFHQFGAEVFGSADPAADAEVISLAKSLFDTLGIDR
                     LRLEINSIGCPECRKNYHKALREYFEQHADGLCETCRGRLERNPMRILDCKSAECKKI
                     AEDAPKVLDYICDDCRTHFDQVKARLSSCQINFTVNPSIVRGLDYYTRTVFEFIDDND
                     GLTVCGGGRYDGLVEQLGGPRLPALGFGLGLERLLMVMESRGCSFDKKEGQRVYLAPM
                     GMNASQKAAELVRLLREEGFTAEYDLMGRSVKAQMKFADKRGFDYTAVIGDNELSESK
                     AMLRNMKTGAVGEIPLDSGIVSAFYRITQKDALEGLCDSVRDS"
     gene            complement(5889..6293)
                     /gene="mgsA"
                     /locus_tag="VHR42_09620"
     CDS             complement(5889..6293)
                     /gene="mgsA"
                     /locus_tag="VHR42_09620"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX3027462.1"
                     /translation="MNIAIIAHDSKKELMVQFCIAYCGILSRNSLCATGTTGKLVSEA
                     TGLEIQRFLSGRSGAQQIAARIACNEIDLLMFFRDPLARKPHEPDEMNMLRLCDVHNI
                     PVATNVATGEVLVRALDRGDLDLRNIVNPKSK"
     gene            complement(6363..>7049)
                     /locus_tag="VHR42_09625"
     CDS             complement(6363..>7049)
                     /locus_tag="VHR42_09625"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3027463.1"
                     /translation="TFSVNCGAALAARGRKVLLVDADAGMRALDLMLSVTRSVIYDLS
                     DVLTGRCEPVKAIIETPVEGLSLLPAPQIVSSELVEPEGMQRLCRGLTHYYDYVLIDS
                     PAGIGSGAETAAAGADKALVVATPDPVCIRDADRIASILLNRGYGQLRLVINRVNPKL
                     IRKKTKTDLDAVIDNAAIQLIGVVPEDERVSVASFSGTTVVSFKKGAAVAYKNIAARL
                     MGEDVPLMKL"
BASE COUNT         1655 a         1929 c         1669 g         1796 t
ORIGIN      
        1 taacatcgcc tattgccagg ctttcacccg aatactgaga tgcaaacgtc gggttgttgt
       61 cgaggttgat atattttatg gtgatatttg aattggtttg ctcgaatttc tcgatgattt
      121 tgatcgtctt ggcgtaatag gagctggcca tcagcacttt tttatccgcc aggatataga
      181 tcgttgtatt cgtttttatg gttttaaggt atttggtcgt ggcggtcgat aagttgtagg
      241 tttttgtgtt ggtaagatca acgcccagtg ttaccttatc gttcacggcg gtcaaaacca
      301 catttaaaag gatcacagca gcaataaaga ttattgtaaa aatagtcgat gtgctgccgt
      361 atttaaagtt ttttctttta aaaaacacgg tcttattctg cttttcctgc agatcttcct
      421 ctttcttttt accgaaaatc atgatcctgc ctcctttcta gctccatctt cttttttcaa
      481 gaacccgcat ggttaaaaac agaaatatgg ccgccatgct gcaatagaat acgacatcgg
      541 ccagattaaa aattccaagg gcgaaattat tatagtgtgt ggtaatcgag atcgctccgg
      601 taatggctga aagaaccgca ttggtggacg aggagaagtt atccgccata aacagcatca
      661 gcataatgaa aaagctggcg actgccgcga tcatctggct ctcggtgagg gccgagataa
      721 aaatgccgat ggcaatcagg gcgcttccta caaaaagcat ggcaacgata tttccgacaa
      781 tcacccagcc gtcaaccgag ctcatgacag cgaggataat tccgaagacg gcaaccgatg
      841 agagcgccat ggcaaacacg caaagggccg ccaaaaattt acctattaca attccgctca
      901 ggctgaccgg agatgtcaga agaatctgat cggttttttg ctttttatcc tcacacatca
      961 gcttcatggt cagtatcgga atcaaaaata cataaataaa aataatgctg ctgaagaagc
     1021 tcgacatatc gttggtgttt ccgccgatat tcacgttaat gaaaaaataa cctgaaaaga
     1081 agaggaacac cgacaggaaa acgtagccta tcgcggacga aaaataagat ttaaactcac
     1141 gtctgaatat tgcgcccatt attccttatc ctccttttcc tttgtttccg gctcctgctt
     1201 cttttggccg gaaagcaggg attccgcctg actgttcagc atctcgtcgg tattgtcttc
     1261 atcgtcttcc ggcgtatccc gctcgatcat tctggtatat ttcgggtcct tctgataatc
     1321 tacctttttc tttttgcgta ttgccggcgc cacatcttcg ctggtcagct tcaggaaaat
     1381 atcttccagc gtcatctcgc cgctgtcgag aaagagcagc ggccacttac ggtcggaaag
     1441 cacgctgaac agagccctgc ggacgtcttt gcccggttcc atttcaatct ggaagtcaaa
     1501 ggaatccttt tcccgaatgc caagcggtgt aacaagcctt accccgctga taccggagag
     1561 cgccctgcgc accgtttcct ccggacccgc gatccgggcc accagcctgt gttcggcgga
     1621 aagattggtc gaaaggttaa acgccgtgtc gtcggcgaca atgcggcctt tattgataat
     1681 gacgatgcgt tcgcacacgc tctgaacctc aggaagaata tgggagctta aaatgacggt
     1741 atgcttttta ctcaggtgct tgatcaggct gcggatctca ataatctgtt tgggatcaag
     1801 accgaccgtc ggctcgtcca aaatcagaac atccgggttg cccacaagcg cctgcgccag
     1861 cccgacacgc tgccggaagc ctttggaaag gtttttgatc aaccttccgt aaacatttgt
     1921 gattttaaca agatcgcaaa tttcggcaag atgagcttcc cttttcagtt tcgtcccctt
     1981 gagctcataa acgaaattca aataatcctt aaccgtcatg tcaagataca gcggaggctg
     2041 ctcgggaaga aatcctatgt gtttctttgc ctgagacgga ttttccagaa catcaattcc
     2101 cgctacctta acgcttccct cgttggagga aagatagccg gtaatgatat tcatggtcgt
     2161 ggattttccg gcgccgttcg gcccgagaaa tcctaaaatt tcgccttctt tgacagaaaa
     2221 gcagatatcg tcgactgcgg ttttgtcgcc gtatcgtttt gtcagatggc tgacctctat
     2281 catagatcgt accctcctgt tttcaataaa atatctatct tgatggccgc gtcaatcagg
     2341 cggcctgaaa aaccatagaa aaacaacctc tttattctat cattacatcc tgctgtattt
     2401 gttaattttt tttaaaactt tattctttaa ttgataaact ttacgatttt tcttgatccg
     2461 tttcggtcag ccggaggtga agctcctcaa tctgcttctg atcgacctcg gccggggagg
     2521 acgacatcag ttcagaggcg ttctgaacct tggggaacgc gatcacttca cggatcgagt
     2581 cgcagccgag catcagcatg gccagacgat caaggccata agccattccg ccgtggggcg
     2641 gcgcaccata tttaaaggcg tcgatcagga agccgaaccg ttctctggcc gtctgctccg
     2701 ataagcccag ggcagtgaac atccttttct gcagctcggt gtcattgatc cggatcgagc
     2761 ctccgccgac ttccacaccg ttgagcacca tatcgtaggc tttcgccctg cagctgccgg
     2821 gatcggattc gatccggccg acgtcttctt ccatcggcgc ggtaaagggg tggtgtttgg
     2881 cggtgtagcg ctgttcctcc gcgctgtatt caaacagcgg aaactcggtt acccataaaa
     2941 actcgaaccg gtctttcggg ataagatcga gttttccggc cacttcgagc ctgagagcgc
     3001 ccagcgactc gagaacaacc tcgttgttct tgtcggccac gatcatgacc acgtcaccgt
     3061 cgtgcgcgtc cagccggtca tagatggccc gtgcctcatc ctccgtcaga aattttgcaa
     3121 acgaggacga cgtgctttcc ccattaagcc ttgtaaacgc aaggccgccg gcccgataag
     3181 tcttcacaaa tacggcaagc ttgtccaatt ccttgcgcgt cagcttctga aacgcgcctt
     3241 tggcgttgat ggcgcgcaca ctgcctccac cttcaatggc agaagtaaat actttaaatc
     3301 cgcagccggc ggcaagatcg gaaagatcgg taatttccat gccgaaacgg gtatccggct
     3361 tgtcgcttcc gaaacgatcc atcgcttcgc ggtaggtcag acgggaaaac ggcacgggga
     3421 tctcaacacc gagaatctcc cggaataccc ggtgaacaaa cccttcgttc acttccatga
     3481 cgtcgtcgct gtctacaaac gacatttcca gatcaatctg ggtgaattcc ggctgacggt
     3541 cggctctgag atcttcatcc cggaaacaac gggcgatctg catgtaacgg tcaaaccctg
     3601 aaatcatcag cagctgtttg tagagctgcg gagactgggg aagagcgtaa aagctgcccg
     3661 gatgcacccg gctggggacg agataatccc ttgcaccttc cggtgtactt ttgatcagaa
     3721 cgggcgtctc gatttccaaa aaaccatttc ggtcaaaata atccctcgcc gttttggcaa
     3781 tccggtgacg cagggcgata ttcctctgca tttccggccg gcgaagatca aggtagcggt
     3841 atttgagccg cagttcttcc ttgacatcgg ttttttcggt gatttcgaat ggcaccgtct
     3901 ctgatttgga taaaatccgc agttcctcca cggcaagctc aacctcaccg gtcggcagtt
     3961 ttgaattacc ggaatccctt tttcttaaaa caccttttgc cgcaagaaca tattccccgc
     4021 gaaccgtgaa ggctttttca aaaacccccg ccggcgtttt ctcgtcaaat gcaagctgaa
     4081 ggattcccgt acgatccctg agatcaagaa agaggatgcc gccaaggtct ctccggcgct
     4141 gaacccatcc gcaaaccgtt atttgctttt cgatatcttc caagcggggc tcgccgcagt
     4201 aattggtacg ttttaagcct ttcatcgttt ccgccattat tgatcctccc gttatcggtc
     4261 ttgaatattt tagctgtctc ttacagaatc gcaaagccct tcgagcgcat ccttctgggt
     4321 aatcctgtaa aatgcgctga cgattccgga atcaagcgga atctcgccta cggcgcccgt
     4381 cttcatgtta cggagcattg ccttcgattc cgacagctcg ttgtcaccga tcaccgccgt
     4441 ataatcgaat ccgcgtttat ccgcaaattt catctgtgct tttacgctgc ggcccatcag
     4501 atcatattcg gctgtgaagc cttcttccct cagcagacgg acaagctcgg ccgccttttg
     4561 ggaggcattc atccccatcg gtgcgagata taccctttgg ccttccttct tatcaaacga
     4621 gcatccgcgg gattccatca ccatcagcag acgctccaga cccagcccaa acccaagggc
     4681 ggggaggcgc gggccgccaa gctgttccac aagcccatcg taacggccgc cgccgcagac
     4741 agtcagaccg tcgttatcgt caataaattc aaatacggtt cttgtgtaat aatcaagccc
     4801 ccggacgatg gacggattaa cggtaaaatt gatctggcag gatgaaagcc ttgctttgac
     4861 ctgatcgaaa tgagttctgc agtcgtcgca gatataatca aggaccttcg gggcatcttc
     4921 ggcgattttt ttgcattccg cgcttttgca gtcaagaata cgcatgggat tgcgttcaag
     4981 cctgccacgg caggtctcgc agagcccgtc ggcatgctgc tcaaaatact ccctaagcgc
     5041 tttgtgatag tttttccggc actccgggca tccaatcgag ttgatttcaa gccgcagccg
     5101 gtcgataccc agcgtgtcaa acaggctttt tgcgagagaa atcacctcgg cgtccgcagc
     5161 agggtcggcg cttccgaaca cttccgcacc aaactgatga aattcacggt atctgcccgc
     5221 ctgcggcttc tcaccccgga aacaggaaat cagataaaaa agtttaagcg gcaaaggctc
     5281 attatgaagg ccgttttgca gaacaagccg gacggtcgag gcagtaccct ccggccggag
     5341 ggtaacactg cgcccgcccc tgtcttcaaa cgtatacatt tctttttgga ctacgtcggt
     5401 ggtttcaccg acgccgcggc tgaaaagctc ggtatattcg aaagtcggga aacggatttc
     5461 gcggaaaccg aacgccgcgg cggtatccct catcgccgac tcaacatact gccactgata
     5521 gactttgtcc ggcagcacgt ccagcgtgcc gcgcggcgcc tgcaattgat tcgccatatc
     5581 gatcttcctt tcttatatcg cgggaaaata ttttttcttt tttcaggggc cgcggatgcg
     5641 caaaaatacc ccgccccggt caagggacga ggttagaacc cgcggtgcca cccttgttaa
     5701 gcagagtttc gcttcactca acgcccttta acgcagggct gtcgtgcggc ttttggtcac
     5761 cgccggctcg cgggtgtctt cactttacgc ggcaaaagca cgcttccagt cgagacgcgc
     5821 tctccctgtc tgcctctgta aaattactct tcccgttctt tgccttcatt ttcagtcttt
     5881 gattaacttt attttgattt gggatttacg atatttctca agtccaaatc tccccggtca
     5941 agcgcccgaa ccaaaacctc gccggtagca acattggtcg cgaccgggat attatgaacg
     6001 tcgcaaagac ggagcatatt catttcatcc ggctcatgcg gcttgcgcgc gagcgggtcc
     6061 ctgaaaaaca tcagaaggtc aatttcgttg caggcgatgc gcgccgcaat ctgctgtgcg
     6121 ccgcttctgc cgctcaaaaa cctctgtatc tcaagaccgg tggcttcgga aaccagttta
     6181 ccggtagttc ctgtcgcgca aagggaattt ctgctgagaa taccgcagta tgcgatacaa
     6241 aactgcacca tcagttcctt ttttgaatca tgggctataa tcgcaatgtt catccatgaa
     6301 cctccattcc ttttcacttt tcaaaaccgt tatcaggctc cggagttctt cctctgagct
     6361 ttttaaagct tcatcagggg cacatcttcg cccatcagcc tggctgcgat atttttgtaa
     6421 gcgacagccg cgcccttctt gaatgagacc acggttgttc ccgaaaaaga cgcaacggat
     6481 acccgttcat cctccggaac aacaccgatc agctggatgg ccgcgttgtc gatgaccgca
     6541 tcaagatccg tcttcgtctt tttgcggatc agcttggggt tgaccctgtt tatgacgagc
     6601 cttagctgcc cgtatccccg attcagaaga atggaagcga tcctgtcggc atcgcggata
     6661 cagacgggat ccggcgtcgc gacaaccagc gctttatcgg cgccggcggc ggcagtttct
     6721 gctcccgagc cgatcccggc cggggaatca atcagcacgt aatcataata atgggtaagc
     6781 ccgcggcaaa gcctctgcat tccttccggc tccacaagct cgcttgaaac aatctggggc
     6841 gcgggcagaa gcgaaagccc ctcgacagga gtttcgataa tggccttaac cggttcgcac
     6901 cggcccgtca gcacatcgga aagatcgtag atcacagacc gcgtcaccga cagcatcaga
     6961 tcaagcgccc tcattccggc gtcggcgtca accaacagca cttttctgcc tctggccgcc
     7021 aaagcggctc cgcagttcac cgaaaatgt
//
LOCUS       DATALY010000350         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_761143,
            whole genome shotgun sequence.
ACCESSION   DATALY010000350 DATALY010000000
VERSION     DATALY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761143"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VHR42_09630"
     CDS             <1..434
                     /locus_tag="VHR42_09630"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533749.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX3027464.1"
                     /translation="NNMANSLIVGGLKTGMSVSVACPEGYEPSPEVLEFAKTMGRSFE
                     LVHDPLKAAEDADVVITDVWASMGQEGEAEKRRKAFAGYQINEELMKKARPDAMVQHC
                     LPAHREEEITAETFEKHAAEIFEEAENRLHAQKAVMCRLMA"
     gene            738..1196
                     /locus_tag="VHR42_09635"
     CDS             738..1196
                     /locus_tag="VHR42_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX3027465.1"
                     /translation="MTGLMIFWLVAVIALAIFEIVTVQLVAIWFSVGALGALAAATLG
                     YNLLCQAVVFIVISAVLLVLTRPMCRKFLTAKKVNTNADRHIGSIAIVQEEINNLAPS
                     GAAIISGVTWMARSVDGSIIHVGEKVRVERIEGVKLIVSKCGSASRTVGV"
     gene            1288..2253
                     /locus_tag="VHR42_09640"
     CDS             1288..2253
                     /locus_tag="VHR42_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3027466.1"
                     /translation="MDPVTVVWIILAIAVIILIVNCFKIVPQAKAYVVERLGAYHTTW
                     GSGMIKFKVPFIDRIAKVISLKEQVVDFPPQPVITKDNVTMQIDTIVFFQVTDPKLYT
                     YGVERPIQAIENLTATTLRNIIGDLELDHTLTSRDVINTKIRLILDEASDPWGIKVNR
                     VELKNIVPPREIQDAMEKQMKAERERRQSVLHAEGEKTSQILVSEGQKEAQILQAEAQ
                     KQSAILHAEGVKEAKIREAEGEAQAIMKVQQALAESIKLLNEASPSDKVIAIKSLEAL
                     AKMADGKATKIIIPSDLQSIASLAVSVKELVTGDEKLQVPAKPAE"
     gene            2713..3231
                     /gene="purE"
                     /locus_tag="VHR42_09645"
     CDS             2713..3231
                     /gene="purE"
                     /locus_tag="VHR42_09645"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX3027467.1"
                     /translation="MELSMQDVKKVAVIMGSDSDLPIVKGCIKVLKGFGIPVEVHVMS
                     AHRTPSAASQFASEAAANGIGVIIAAAGKAAHLAGVLAAHTTLPVIGIPIKSSTLDGL
                     DALLSTVQMPKGIPVATVAIDGAENAGILAAQILAVGNAEITAKLLAAKKAMAEEVIK
                     KDFQIQAEVENI"
     gene            complement(3265..3507)
                     /locus_tag="VHR42_09650"
                     /pseudo
     CDS             complement(3265..3507)
                     /locus_tag="VHR42_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353102.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
BASE COUNT          902 a          833 c          960 g          812 t
ORIGIN      
        1 gaaacaatat ggctaactcc ctgattgtcg gcggtcttaa aacgggcatg agcgtttcgg
       61 tcgcctgccc cgagggttat gagccttcgc cggaggtgct ggagtttgcg aaaaccatgg
      121 gcaggtcgtt tgaattggtg cacgatccgc tgaaagccgc ggaggatgcc gatgtggtta
      181 tcactgacgt ctgggcttcg atggggcagg aaggcgaggc ggagaaacgc cgcaaagctt
      241 ttgcaggcta tcagatcaac gaggaactga tgaaaaaggc cagacccgac gccatggtgc
      301 agcattgcct gccggcgcac cgcgaggagg aaatcaccgc cgaaaccttt gaaaagcacg
      361 ccgctgaaat cttcgaggaa gccgaaaaca ggcttcacgc gcagaaagcg gtcatgtgca
      421 ggctgatggc ataaaggccg ggttttctga gcgggtgcag ttcaataaac cagtaatagc
      481 aaaccgcacc aaaaaaaaag cagcgcaaag tgccttgaga agggtttttc atagcccatc
      541 aaggcacttt tttgtacagt attttgtatg acgctgtttt gcagcacccg cttattggag
      601 tctttggaaa ttacagaaaa agtaatttcc tgaagtccgc tgtgttgtgt tttaaggggg
      661 gaagtgctac aatagtctta ttcacccgtt attttcgtta acgaatatcc agccgctttg
      721 cggcagaatg gagtaatatg accggtttga tgatattctg gcttgtggcg gttatagcgc
      781 ttgctatctt tgaaattgtg acggttcagc ttgtcgccat ctggttttcg gtcggcgccc
      841 tgggcgctct cgcagccgcg acgctcgggt ataaccttct gtgtcaggct gttgtgttta
      901 tcgtcatttc cgcagtcctg ctggttctga cacggccaat gtgcagaaag tttctcactg
      961 caaaaaaggt caacaccaat gccgacaggc atatcggcag catagccatt gttcaggaag
     1021 aaatcaacaa cctcgcaccg tccggcgctg ccattatttc gggcgttaca tggatggcaa
     1081 ggtcagttga cggatcgatc attcatgttg gcgaaaaggt cagggtggag cggatcgaag
     1141 gcgtaaagct gatcgtatca aaatgcggct ctgcctccag aacggtcggc gtctgacgca
     1201 caaaacccgg ataaaaataa cgtgtcagtg ttccggcggg cagaatcctg tatccgccgt
     1261 attttaaaac taggggaggc attaattatg gatccggtaa ctgttgtctg gattatcctt
     1321 gcaatcgccg ttatcatcct cattgtcaac tgctttaaaa ttgtgccgca ggcaaaggct
     1381 tatgtcgttg aaaggctggg agcctatcac acgacctggg gctcgggcat gatcaagttc
     1441 aaggttccct ttatcgaccg gattgccaaa gtgatatctt taaaagagca ggtggtggat
     1501 tttccgcccc agccggttat caccaaggat aacgtcacga tgcagatcga caccattgtc
     1561 ttctttcagg tcacagaccc gaagctttat acatacggcg tggagcggcc gattcaggct
     1621 attgaaaatc tgacggctac gacgctgcgt aatatcatag gtgatctaga gcttgaccat
     1681 accctgactt cccgcgacgt aatcaatacg aaaatccgcc tgattcttga cgaggcgtcc
     1741 gacccgtggg gcatcaaggt gaaccgtgtc gagctgaaaa acatcgtccc gccgcgtgaa
     1801 attcaggacg cgatggaaaa acagatgaag gccgaacgtg aacgccgcca gtcggtactg
     1861 cacgccgagg gcgaaaagac cagtcagatt ctggtttccg agggccagaa ggaagcccag
     1921 attctgcagg ccgaggcaca gaagcagtcg gccatcctgc atgccgaggg tgttaaagag
     1981 gccaagatcc gcgaagccga aggcgaggcg caggcgatta tgaaggtgca gcaggctctt
     2041 gccgaaagca tcaagctgct caatgaggct tcgccctcag acaaggttat cgcaatcaaa
     2101 tcccttgagg cgcttgcaaa aatggctgac ggaaaagcca ccaagattat cataccgtcc
     2161 gatctccagt cgattgcttc gcttgccgtt tcggtaaagg agcttgtgac aggcgacgaa
     2221 aagcttcagg ttcccgccaa accggcagaa tgattttgta ttcacaaaat atcgtgaatc
     2281 ctgtcgtata attagagaca tattacacaa agaaagaatt tgcacttgaa aagaggacgt
     2341 ttttacaata aaatagatat agaaaaggta aaatccgtgt aacgcagatt agtttggcgg
     2401 cacacagaaa gaaactttaa cccaatgagg tgtttaagtt gtccgtattg tttttttcag
     2461 gcgagagtta ttctatgtgc tgtaaacgtt gcggtaatgc agactcatgt tcttttacaa
     2521 aataagtgca gctaaataca cttccttgga agtgtatttt ttttctctgg atttttgacg
     2581 cgttaatttt ctgcaggatt gggtaaaatt acatacagaa caacagcggc agccgccgct
     2641 tgcaggatac taaagtccgt ttcaccgccg gaaccgggct ggatttttca ttttaatttg
     2701 tgaaggcgga gaatggagct aagtatgcag gacgtaaaaa aagttgcggt catcatgggc
     2761 agcgatagcg atcttccgat tgtcaagggc tgtataaagg tgttaaaggg gtttgggatt
     2821 ccggtcgagg tgcacgtcat gtcggcgcac agaaccccgt ccgcagcctc acagttcgcc
     2881 tccgaggcgg ccgcaaacgg catcggagtt attatcgcgg ctgccggcaa agcggcgcac
     2941 ctcgcgggcg tgctcgcggc gcacacgacg ctgccggtga tcggaatccc catcaaatca
     3001 tcgacgctgg acggattgga cgcgctgctt tccaccgtac agatgccaaa gggcataccg
     3061 gtagccaccg tggcgattga cggagcggaa aacgcgggta ttctcgcggc tcagattctg
     3121 gcggttggca atgccgaaat taccgccaag ctgctggctg cgaaaaaggc catggcggaa
     3181 gaagtaatca agaaggactt tcaaattcag gcggaggttg aaaatatatg aaaaagctgg
     3241 agcagattta cagaaaccgc ttcccgagtc catcacaatt ttgtcgagat cttcgattct
     3301 cagataaccc agcgtgtcgg ccccgatcgt tttgcagatc ccgtcaaccg acatgcggca
     3361 ggcaatcaga ttgtcgcggg aatcaatatc ggtgccgaag taacaggggt tggtaaacgg
     3421 cggcgccgaa accctcatat gaacctcggt tgccccggcg gagcgcagca gcgagacgat
     3481 gcgcgcgctc gtcgttcctc tcacgat
//
LOCUS       DATALY010000351         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_253042,
            whole genome shotgun sequence.
ACCESSION   DATALY010000351 DATALY010000000
VERSION     DATALY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253042"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VHR42_09655"
     CDS             <1..206
                     /locus_tag="VHR42_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX3027468.1"
                     /translation="LFCGVTNAPLATIVLCMELFGAKGLPFFALAVAVSYQISGNGGL
                     YSAQEIVSNKLAIMQKGDEKINE"
     gene            203..1159
                     /locus_tag="VHR42_09660"
     CDS             203..1159
                     /locus_tag="VHR42_09660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX3027469.1"
                     /translation="MNMIILDLEWNGVYTKKTKGYFNEIIEVGAVKMNDRMEMIDTFH
                     AVIKPVVSRKLSSLVQNLTGIAQEELEDGAQFPRAVSQLRKWIGDPAAVVMTWSTTDL
                     MVLLENCRYFLDDERIPFMHYYADLQAYCQMRMEKSTEEKLENAGQQLGLGKACEMLG
                     IHEDELSPHRALDDSILSGRVFANIYETNTFGKVVYAADQAFYERLAFKNTIISDIND
                     EHIRHSDLRFCCDVCSHPLRRTSEWQFRSRAFCADFWCRHCDQKYIGRVQVKLKYDGP
                     EIKKKLIVRPAEQEATSEEPAQSAENSAVADKADTSSPDFKE"
     gene            1271..1753
                     /gene="mscL"
                     /locus_tag="VHR42_09665"
     CDS             1271..1753
                     /gene="mscL"
                     /locus_tag="VHR42_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX3027470.1"
                     /translation="MFGEFKTFALRGNVMDLAVGVIIGGAFQKIVTSAISNLITPLIG
                     CVTGRVNFDNLFIVLSNTSSTPTDQIKTLDQANKLAVATFNYGAFITNLLDFVIMAFV
                     VFLMVKGINRLTSISLIKKPEAAAKTKKCPYCCSDIDVKATRCPHCTSKLGGETADGE
                     "
     gene            1950..>2502
                     /locus_tag="VHR42_09670"
     CDS             1950..>2502
                     /locus_tag="VHR42_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3027471.1"
                     /translation="MSKLLNGKKGVLAFVGCLALVAASIGAVRYVENKVQTTAASAAN
                     TNWGLSFRAEGQTPIGNASSEFLKQYDAYYVGNTSKKVIYLTFDAGYENGYTASILDT
                     LKKHKVKATFFVVGHYIETSPDLVKRMCAEGHIVGNHTYHHYDMSKISDITSFTQEMK
                     SVEELYKKTTGQQMQKFYRPPQGK"
BASE COUNT          693 a          560 c          648 g          601 t
ORIGIN      
        1 cgctcttctg cggcgtgacc aacgcgccgc ttgccaccat tgtactgtgt atggagctgt
       61 tcggcgcaaa gggattgccg ttttttgcgc ttgcggtggc ggtaagctat cagatatcgg
      121 gcaacggcgg attatattcc gcgcaggaaa ttgtgagcaa caagctggcg attatgcaaa
      181 agggggatga aaagataaac gaatgaacat gatcattttg gatctggaat ggaacggcgt
      241 gtatacgaaa aaaacaaagg gatattttaa cgaaatcatt gaagtcggcg ccgtcaaaat
      301 gaatgaccgg atggaaatga tcgatacctt tcatgccgtg atcaaacccg ttgtcagccg
      361 taaactttct tctctcgtac agaaccttac gggaatcgca caggaggagc tggaggacgg
      421 cgcacagttt ccccgtgcgg tatcccaact gcgcaaatgg atcggcgatc ccgctgccgt
      481 cgtaatgacg tggagtacaa cggacttaat ggttctgctt gaaaactgcc ggtatttttt
      541 ggacgatgaa cgtatccctt tcatgcatta ttacgccgat ctgcaggctt actgtcagat
      601 gcgaatggaa aagagcacag aagaaaagct tgaaaatgcg gggcagcagc tgggtcttgg
      661 taaagcctgc gagatgctcg gtattcatga ggatgaattg tctcctcatc gggctctgga
      721 cgacagtatt ctgtcggggc gggtattcgc caatatatat gaaacgaata cctttggtaa
      781 ggtggtttat gcagccgatc aggcgttcta tgagcgcctt gccttcaaga ataccattat
      841 cagcgacatc aacgatgagc atatccggca ttccgatctt cgcttttgct gtgatgtctg
      901 cagccacccc ctgcgccgca cgagcgaatg gcaattccga agccgggcat tctgtgccga
      961 cttctggtgc agacactgcg atcagaagta tatagggcgt gtgcaggtga aactgaaata
     1021 cgacgggccg gaaataaaga aaaagctgat cgtaagaccg gccgaacagg aagccacgag
     1081 cgaggaaccc gctcaatcgg cagagaattc cgcagtcgct gacaaggcag atacttcatc
     1141 gccggatttt aaggaataag ctatccatgt atttagtatg gaagaatgaa aaaaaggttt
     1201 caatgaaagg atggtcacta tgaaaaaacc gaatattgaa atacctgtag caatcggaaa
     1261 aaagctgtct attttcggtg aattcaaaac cttcgctctg cgcggcaacg ttatggattt
     1321 ggccgttggc gttatcatcg gcggcgcatt tcagaaaatt gtaacgtctg cgatcagtaa
     1381 cctgatcacg ccgctgatcg gctgcgtgac cggccgcgta aatttcgata atctttttat
     1441 agtactgagc aatacgagca gcacccctac cgaccagatc aagacgcttg atcaggcaaa
     1501 caaattagcg gtggcgacct tcaattacgg cgcgtttatc acgaatctcc tggattttgt
     1561 gattatggcg tttgttgtat tcctgatggt caagggcatc aaccgcctga ccagcatcag
     1621 tcttatcaag aagcccgaag cggctgcgaa aacgaaaaag tgcccctatt gctgcagcga
     1681 catcgatgta aaagcaacgc gttgtcctca ctgcacatct aaacttggcg gtgagactgc
     1741 cgacggggaa tagagttaat ctcctacagt aaaaattaag gtaaagtaca tataattccg
     1801 gatgagcagt ttggtttcat ccggatttta ttttttatac aggactgtcc ggaaaatcgg
     1861 cttcggacaa ggtcccacaa gcatgtttcc ggtttgtctg ccatatagat ataccgtcac
     1921 cgaatatgat ggaatcgatg gtggggagca tgagtaaact gttaaacggg aaaaaagggg
     1981 ttctggcctt tgtcggctgc cttgccctcg ttgccgcgtc gataggcgcg gtccgttatg
     2041 tggagaataa ggtgcagaca accgccgcct ccgccgctaa tacgaactgg gggctgagtt
     2101 tccgcgcaga ggggcagacg ccgatcggca atgcatcatc cgagttttta aagcagtatg
     2161 acgcatatta tgtaggaaat acgtcgaaaa aggttattta tttgacgttt gacgccggat
     2221 atgaaaacgg atataccgca tccattctgg atacgctgaa gaagcataaa gtgaaagcga
     2281 cgttttttgt cgtcgggcat tacatcgaaa ccagccccga cctcgtaaaa cggatgtgtg
     2341 cggaaggaca tattgtgggc aatcatactt atcatcatta cgatatgtca aaaatatcgg
     2401 acattacttc ctttacccag gaaatgaaat ccgtcgagga gctgtataaa aaaacgaccg
     2461 ggcagcaaat gcaaaaattc taccgcccgc cccagggcaa at
//
LOCUS       DATALY010000352         4781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_507960,
            whole genome shotgun sequence.
ACCESSION   DATALY010000352 DATALY010000000
VERSION     DATALY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507960"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHR42_09675"
     CDS             complement(<1..252)
                     /locus_tag="VHR42_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3027472.1"
                     /translation="MGRIIGIDLGTTNSCVAVMEGGDPVVIPNPEGARTTPSVVAFSK
                     NDERLVGQVAKRQGITNPDRTVISIKRNMGTDFKVKIDNK"
     gene            complement(314..991)
                     /gene="grpE"
                     /locus_tag="VHR42_09680"
     CDS             complement(314..991)
                     /gene="grpE"
                     /locus_tag="VHR42_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX3027473.1"
                     /translation="MAKKKDPNNGPEQKGKKSCNDNSDTPINDTTAAGPEQKAGKDND
                     KAGEKKVSENDVLKLYLEQAISELKKTKSQLEESKKLEEQAKDEVKTYQEKLKSLAAE
                     YENYRRRTSAEKESINADAIAKAVTALLPALDSLEKAAEFTQSNPESIRQGLEMTLKQ
                     LSEGFANLGVSEIDAKGAGFNPDLHNAVMHVEDETLGESVVTDVFQKGYQIGEKVIRH
                     SVVKVAN"
     gene            complement(1066..2097)
                     /gene="hrcA"
                     /locus_tag="VHR42_09685"
     CDS             complement(1066..2097)
                     /gene="hrcA"
                     /locus_tag="VHR42_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX3027474.1"
                     /translation="MELGERKKKILNAIVETYIATGEPVGSKVIAQITDMGLSSATIR
                     NEMSELAEMGFIVQPYTSAGRIPTQKGYRMYVDRLMDKYQLNFDEKREIDNLLKINGG
                     DIESTLSKAGELLAQITGFASLSTSCQDRRNRVRRIQVVPAGRRSLLLVILATSGVIK
                     TRLCRIDEDLTEDMLQFFSRLVNDKVSGKTFEEVTPGLMDAVSGELFEYTYALRPVLG
                     IILEEIRDIMDAEVFLGGEANLLAHREFDGTKVAEIIRYLEKRDEILRLIEDIGGGIS
                     VRIGTENGISPMEDSTLIAAPYDFQGRTAGTIGIIGPTRLDYARLMSNIEYFSTVLSR
                     LINEAFGDV"
     gene            complement(2214..3323)
                     /gene="hemW"
                     /locus_tag="VHR42_09690"
     CDS             complement(2214..3323)
                     /gene="hemW"
                     /locus_tag="VHR42_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX3027475.1"
                     /translation="MEDSLGIYIHIPFCEGGKCPYCDFYSVAFDSQRAEEYAAAVGRL
                     LAVWGQKAGGRVVDTVYFGGGTPSLMGKWLTWMLSCVRRNFNLSADAEITLEANPGRN
                     LYDILPGLRKSGFNRLSIGLQSAHPQELKLLGRRHTPMDAARAVEDAKRAGFENVSLD
                     LMFCLPGQTIQSLRESIDFAAGLSVQHLSAYILKIEPGTPFDLQRSRMNLPDEDEQGE
                     MYLAACDAIEAKGYRQYEISNFALADKQSRHNLKYWNCEEYLGIGPGAHSFFGGKRFF
                     YPRDLRSFLTKALSVDDGGGGSVEEYVMLRLRLSEGILRSELLRRYQRDFGMFDPKKI
                     QDLEKAGLVAADENQIRLSKKGMVVSNSVISYLIF"
     gene            complement(3399..>4781)
                     /gene="lepA"
                     /locus_tag="VHR42_09695"
     CDS             complement(3399..>4781)
                     /gene="lepA"
                     /locus_tag="VHR42_09695"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX3027476.1"
                     /translation="ADPDRAVKEIEDIIGIPAADAPRISAKMGLNIGQVMETIVSSVP
                     APKGDSSAPLRALIFDSYYDSYRGVITYIRVVEGKIGPGTRIKMMATGAQFDVVEVGY
                     MRPTSLEPAKSLGAGEVGYVAASIKNVSDTRVGDTITTVTGGATEPLPGYKKVNPMVF
                     CGIYPADGSKYEDLRDALSKLQLNDASLVYDPETSVALGFGFRCGFLGLLHMEIIEER
                     LEREYNLDLITTAPSVVYRITRTNGEVQYVDNPTNYPDPGLIQMAEEPIVNAHVLTPS
                     QFVGNIMELCQERRGTFIDMKYIDTDRVDLHYELPLNEIVYDFFDALKSNTKGYASFD
                     YEVLGYRESNLVKLDILLNSETVDALSFIVHADKAYGRGRKITEKLKDKIPRQLFEVP
                     IQAAIGGKIIARETVRALRKDVLAKCYGGDITRKKKLLEKQKEGKKRMRQLGSVEVPQ
                     EAFMSVLKLD"
BASE COUNT         1089 a         1361 c         1075 g         1256 t
ORIGIN      
        1 tttattatca attttgactt taaaatcggt acccatgttc ctcttgatcg atatgacggt
       61 gcggtcgggg tttgtaatac cctgtctttt ggcaacctgg ccgaccagac gctcatcgtt
      121 tttggaaaaa gcgacgaccg acggggtagt tctggcgcct tccgggtttg ggataacgac
      181 cggatcgccg ccctccataa ccgctacgca cgagtttgta gttccaagat caattcctat
      241 gattctgccc atgtgatata cctcctgatg gatattcttt attcataatc aatttaatga
      301 aattgccgtc tgtttaattt gccactttga ccaccgaatg gcggatcact ttttcgccaa
      361 tctgatagcc tttctgaaaa acgtcggtta caaccgactc gccaagagtc tcatcctcaa
      421 catgcatcac tgcattatgc agatccgggt taaaccccgc ccctttggca tcgatctcag
      481 aaaccccgag atttgcaaag ccttcggaaa gctgtttcag cgtcatttca aggccctgcc
      541 tgatactttc agggttcgac tgggtaaatt cagccgcttt ctcgagactg tcaagagccg
      601 gcagcagcgc agtcaccgct ttcgcgatcg catccgcgtt aatgctttct ttttcggcgg
      661 aggttcttct tctgtaattt tcatattccg ccgcaagcga cttcagcttc tcctgatagg
      721 ttttgacttc atcctttgcc tgctcttcaa gctttttgct ctcctcgagc tggctctttg
      781 tctttttcag ctctgaaatt gcctgctcta gatacagctt cagaacatcg ttttcagaaa
      841 cttttttctc cccggccttg tcattatcct taccggcctt ttgctccggc ccggccgcag
      901 tcgtatcatt aatcggtgta tcgctgttat cgttgcagct ttttttgcct ttttgctcgg
      961 gcccgttgtt gggatctttc tttttcgcca aattaaaatc tcctttctgt tgtcgggttc
     1021 tatttctaaa acgtattaac gtgttatcgc ttttccgcgc ggcgttcaaa cgtcgccgaa
     1081 tgcctcgtta atcagcctgc tcagcaccgt cgaaaaatat tcaatgttgg acataagcct
     1141 tgcgtaatcc aatctcgtcg ggccgataat tccaatcgtg ccggccgtcc tcccctggaa
     1201 gtcgtaaggc gcggcaatca gagtggagtc ctccatcggg ctgattccgt tctccgttcc
     1261 gattctgacg ctgatgccgc cgccaatatc ttcgatcagc cgcaggattt cgtccctttt
     1321 ttcaagatac cggataattt cggccacttt tgtgccgtca aattcccgat gggcaagaag
     1381 atttgcctcc ccaccaagga acacctcggc atccatgata tcccgaattt cctccagaat
     1441 gatgcccagt acgggcctga gcgcataagt atattcaaaa agttccccgg aaacagcatc
     1501 catcagcccg ggagtaacct cctcgaacgt tttcccgctt actttgtcgt taaccagacg
     1561 cgaaaaaaac tgaagcatgt cttcggtcag atcttcgtca atacggcaaa gccttgtctt
     1621 gatgacgccg gaggtagcaa gaataaccag cagcagagac cttctcccgg ccggcaccac
     1681 ctgaatcctg cgaaccctgt tgcgcctgtc ctgacaggag gtggatagcg acgcgaagcc
     1741 ggtgatctgg gcgagaagct cccctgcttt tgaaagggtg ctctcaatat ctccgccgtt
     1801 tatcttcagc agattatcaa tttcccgttt ttcatcaaag tttaactgat atttatccat
     1861 cagcctgtca acgtacattc ggtagccctt ttgcgtgggt atccggcccg cggaagtata
     1921 gggctgaaca ataaagccca tttcagccag ctccgacatt tcattgcgga tcgtggcgga
     1981 agaaagcccc atatccgtaa tttgggcgat caccttcgag ccgaccggct cgcctgttgc
     2041 aatatatgtc tcgacaatag cgtttaaaat cttcttcttt ctttcgccta attccaaatt
     2101 tatcaccccg ggtgttcgac ctgtttctct tttgattagc gttagcactc ctatacctcg
     2161 agtgctaaca ttaagaatgt atcaccaaaa ctgtacgttg tcaataccct gcgttaaaaa
     2221 atcaggtagg aaatcaccga attgctgact accattcctt tctttgacag ccggatctga
     2281 ttttcatccg ccgcaacaag ccccgctttt tcaagatctt gaattttttt cgggtcaaac
     2341 atgccgaaat ccctctgata acgccgaaga agttcgctcc ggagaatccc ttcgctgagc
     2401 cgcagccgga gcatgacata ctcttccaca ctgcctccgc cgccgtcatc aaccgacagc
     2461 gcctttgtta aaaacgagcg gagatcacgc ggataaaaaa aacgctttcc accaaaaaaa
     2521 gagtgggcgc cgggtccaat tccaaggtac tcctcacaat tccagtattt cagattatgc
     2581 cgcgactgtt tatccgcaag ggcaaagttt gatatttcat actgccggta tccctttgcc
     2641 tctatggcgt cacaggccgc cagatacatt tctccctgct cgtcttcgtc ggggaggttc
     2701 atccggcttc tctgcagatc aaaaggcgta cccggctcaa ttttaagaat ataagccgac
     2761 agatgctgaa ccgacagccc ggccgcaaag tcaatcgact ccctgaggct ctgaatcgtc
     2821 tggcccggca gacaaaacat cagatcaagt gaaacattct caaacccggc cctttttgca
     2881 tcttccaccg cgcgggccgc atccataggg gtgtgccgtc ttcccagcag cttgagctcc
     2941 tgagggtgtg cggattgaag cccgatggaa agccggttaa acccggattt gcgtaatccc
     3001 ggtaaaatat cgtacagatt ccggcccgga ttcgcttcca gcgtaatctc ggcatcggcc
     3061 gagagattaa agttccgcct gacacagctc agcatccagg tcagccattt acccatcagt
     3121 gatggagttc cgccgccaaa gtagacggta tcgacaaccc tgccgccggc cttttgcccc
     3181 catactgcaa gaagccgccc gactgccgcc gcatattctt ccgcgcgctg cgagtcaaac
     3241 gcaaccgaat aaaaatcaca ataaggacat ttaccgccct cgcaaaacgg gatgtggata
     3301 tagataccca gactgtcttc catgaaaacc tccgcaaaaa tccgcagagg gcggcccgaa
     3361 tccgcatttc gcgtcgggca gccctctgcc ggagtggact aatccagttt taataccgac
     3421 ataaacgctt cctgaggaac ttcgacactg ccgagctgcc tcatgcgctt tttgccctct
     3481 ttctgcttct ccagcagttt tttctttctg gtaatatcac cgccgtaaca ttttgccaaa
     3541 acgtctttcc gcaacgccct gactgtttca cgggcaatga ttttgcctcc gatcgccgcc
     3601 tggatcggca cttcaaacaa ctggcgggga atcttatcct tcagcttctc ggtgattttt
     3661 ctgcccctgc cataggcttt gtcggcgtgg acaataaagg aaagcgcgtc caccgtctcg
     3721 ctgttgagca gaatatccag tttcacaagg ttggattccc gatagcccag cacctcataa
     3781 tcaaaggaag catagccctt cgtgttggat ttcagcgcat caaaaaagtc ataaacgatt
     3841 tcattaagag gcagctcata atgaagatca acccggtcgg tatcaatata tttcatatca
     3901 ataaaggttc ctctgcgttc ctggcagagc tccatgatgt tgccgacaaa ctgcgacggc
     3961 gtaagaacat gggcgtttac aatcggttcc tcagccatct ggataaggcc cgggtcagga
     4021 tagttggtgg ggttgtccac atactgaacc tctccgttgg tgcgggtgat tcggtaaaca
     4081 acgctcggcg ccgttgtaat caggtcaaga ttatattcgc gctccagccg ctcctcgatg
     4141 atctccatat ggagcaggcc gagaaaaccg cagcgaaatc cgaacccaag cgccaccgag
     4201 gtctcagggt cgtaaacaag agacgcatcg ttaagctgaa gtttggaaag cgcatcccgc
     4261 agatcttcgt attttgagcc gtcggcggga tagatgccgc aaaaaaccat tggattaacc
     4321 tttttataac ccggcagggg ctccgtagcg ccgccggtca cggtcgtgat cgtgtcaccg
     4381 acccgcgtat ccgatacatt tttaatgctg gccgccacat aacctacttc gccggctccc
     4441 agacttttgg ccggctcgag gctggtgggc cgcatatagc caacctcaac cacatcaaac
     4501 tgcgctcccg ttgccatcat tttgatacgg gttccgggcc cgatttttcc ttccaccacc
     4561 cggatatagg taataactcc gcggtagctg tcgtaatagg aatcaaagat cagcgcgcgg
     4621 agcggagcgg aactgtctcc ctttggagcc ggaacggagc ttacgatggt ttccatcacc
     4681 tgaccgatgt tcagacccat tttcgccgaa attctcggtg cgtccgccgc cggaatgccg
     4741 ataatatcct cgatctcctt gaccgcgcgg tcagggtcgg c
//
LOCUS       DATALY010000353         5037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_851050,
            whole genome shotgun sequence.
ACCESSION   DATALY010000353 DATALY010000000
VERSION     DATALY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851050"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..970)
                     /locus_tag="VHR42_09700"
     CDS             complement(200..970)
                     /locus_tag="VHR42_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027477.1"
                     /translation="MHFKRLLCTVALALTMSFSFGLVQAASPGLIESNKEVNTETLVN
                     EIKSDKAANVSEINDLLDRCKNNDQVAIQQIQSFDCFNNKMVNDSTNDMKIDPNSSRT
                     ITFDDGSSITYTNTLTSANNFNLFSLSRSLTSSTSMVQLSSYSFPTPAKTSKITAEYK
                     VGPIVVGHHGGSATWCDYSRTKCHLYSTNPVLDGWGISISNPSSKIVINNASSAVFSV
                     TATLSLSQAGITFFSVHGTDVMFIETAGAMGFRSQGLA"
     gene            complement(1477..1695)
                     /locus_tag="VHR42_09705"
     CDS             complement(1477..1695)
                     /locus_tag="VHR42_09705"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX3027478.1"
                     /translation="MRKYIIDRFEKGYAVLEEENGHLIDMAAAQLPREAKEGDVILMK
                     SGGSFEIDREETNRRHRIIQDFQKRLMD"
     gene            complement(1720..2655)
                     /locus_tag="VHR42_09710"
     CDS             complement(1720..2655)
                     /locus_tag="VHR42_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX3027479.1"
                     /translation="MARQKKRLTPLGIIAAVAVLAFVLFANLGDRNVFPGWLQNPVNT
                     ANSATGHLIFPDQPAKIENLEVCFFDVGQADSIFVSCDGATMLIDAGNPDDSDTVENN
                     LKRLGVTSLDYVVGTHPHSDHIGGLSQIISDFNVKEIMMTGTANNTNVYYNLLKTIQS
                     KGKKITKPAVGKTTKFGGGSFTVDAPNAAYEDMNNDSIVIRFTYHKLSFLFTGDASKD
                     SEKDMLSRHDNLQADVLKVGHHGSQTATSAAFLKAVNPRYAVISVGASNSYGLPDESV
                     ISRLENAGVKVFRTDENGTVIFKSDGDTITCNTEK"
     gene            complement(2631..4538)
                     /locus_tag="VHR42_09715"
     CDS             complement(2631..4538)
                     /locus_tag="VHR42_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEX3027480.1"
                     /translation="MSGFLPVSKEDMAERGWYYLDFICVTGDAYVDHPSFGPAIISRV
                     LESEGFKVGIIAQPDWKTTDGFQKLGRPRYGFLVSSGNIDSMVNHYTAAKKPRAEDFY
                     SPGGKPHCRPDRTVTVYTQKIKEAYPDVPVIIGGLEASLRRFAHYDYWDDRVRPSILM
                     ESGADLLVYGMGERQTAELSNRLRRGENISGLTDIRGTCYLSKNIPAENTAICPSCER
                     VSSDKAAYAKSCRIQYEQQDAVTGKTVAQDHGGVVLIQNPPALPLNTQELDRVYSLPY
                     MRAWHPVYDSQGGVPAIQEVEFSITHNRGCFGACNFCAISFHQGRMITSRSRESVLEE
                     AQKLIEKPNFKGYIHDVGGPTANFRKPSCKRQLAFGLCKGKKCLAPVPCKNLEVDHRE
                     YLDLLRSLRSLPGVKKVFVRSGLRYDYMMEDPDDSFFRELVKHHVSGQLKVAPEHCSA
                     AVLDRMGKPHIETYVKFMKKFYDITKKAGREQYLVPYLMSSHPGSTLKEAVELALFLK
                     KEHIRPEQVQDFYPTPGTVSTCMFYTGLDPFTMKKVYVPRTPGEKAMQRALLQYFKPE
                     NRRIVLDALRQAGRTDLIGFSGECLVRPDARAQTNVRKPQIRPAEKRGGHPKGKKKRG
                     ALGAWQGKRNV"
     gene            complement(4551..>5037)
                     /locus_tag="VHR42_09720"
     CDS             complement(4551..>5037)
                     /locus_tag="VHR42_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX3027481.1"
                     /translation="VDSRTILDMGGAEKLLGRGDMLFLPVGASKPVRVQGCFVSDNEV
                     ESVIEYIKNGADSNYSEDIMDEIDKQSAKDKSGGEDSRGGDADAMLPQAIECVIEAGM
                     ASTSFLQRRLKLGYARAARIVDEMEARGIVGPFEGSKPRQVLITRQQWIEMTMNEDGK
                     E"
BASE COUNT         1270 a         1264 c         1095 g         1408 t
ORIGIN      
        1 tatattcatt tctacaacta tgaacgcata cagactaaaa atatgctgac accgtatcaa
       61 atgcggtgcc agcacgagta atctaaacct attctacgat ttgggtgctt tttccgtgtc
      121 taattaacag ggttcggtgc aaattgcttg gctgcttgta atttaaatta ctatcaaata
      181 gccatagcgt atcatccaat caagctagac cttgacttct aaatcccatg gctcctgctg
      241 tttcaataaa cattacatct gttccatgta ctgaaaaaaa tgtgatacct gcttgtgata
      301 aagataaagt cgcggtaact gaaaataccg ctgatgaagc gttattgata actattttac
      361 ttgaagggtt tgaaattgaa attccccatc cgtctaatac tggatttgta ctatataagt
      421 ggcactttgt gcgagaataa tcacaccaag tagcagatcc accatgatga ccaacaacta
      481 tagggccaac tttatattct gccgtaattt ttgatgtttt tgcaggagtt ggaaatgaat
      541 atgaagataa ttgtaccatg ctagtggaac tagttaatga tctagataag gaaaaaagat
      601 taaaattatt ggctgaagtt aacgtattag tataagttat actactccca tcatcaaaag
      661 taattgttct cgacgaattt ggatcaatct tcatgtcgtt cgttgaatca ttaaccattt
      721 tattattaaa acaatcaaat gattgaattt gttgtattgc tacttgatcg ttattcttac
      781 accggtctaa caagtcatta atttcagata cattagcagc tttatcactt ttaatttcat
      841 ttacaagtgt ttctgtattt acttctttat tgctttcaat taaaccggga gaagcagctt
      901 gaactaaacc aaatgaaaat gacatggtca acgctagcgc tacggtacat agcagtctct
      961 taaagtgcat aattatccat ccttttttaa tattattgat tcaatagtta aatattagca
     1021 tttttttgtt atgttttatc aagatatatt ttctatttta tagtatatat tgacaatttt
     1081 tatattttat tatagtataa taatcaagag attcatggat aggagggaat aatttgatgg
     1141 gtatgatttt ttcaatatat ttttctgtaa tcattttgtt atcattaatt gggatcgccc
     1201 taattgtatt attattcact gtacttatta aagcgaataa atttctaaat ctaaaattag
     1261 atcaggaaaa gaaaaaaatt aattaggata atagtagaaa aagcctggat aaatccaggc
     1321 tttttctact atacggttat cagacgcccg caatttgaca gccatattaa aaaaatcccg
     1381 tacaaagcac gggattttct catggaatca acttaaattt cgctattgcc ggggaatttt
     1441 aaaatcaggg cacgcgattg gctcaacccg cttatttcaa tccatcagcc ttttttggaa
     1501 atcctgaatg attcggtgcc gcctattggt ttcctcccgg tcgatttcaa acgacccgcc
     1561 ggatttcatc agaatcacgt caccctcttt tgcctcccgc ggaagctgcg ccgcagccat
     1621 gtcaatcaga tgcccgtttt cttcttcaag cactgcgtag cctttttcaa accggtcgat
     1681 gatgtatttt ctcataatta tcccccgtaa tattttccat tatttctctg tattacaggt
     1741 aatagtatcc ccgtcacttt tgaaaataac cgttccgttt tcatccgttc tgaaaacttt
     1801 tacaccggca ttttccagcc tgctgatcac cgattcatcc ggaagaccgt aactgttgga
     1861 ggccccgacg ctgatgacgg cgtagcgcgg attcaccgcc ttcaggaaag cagccgaggt
     1921 agccgtctgg ctgccgtgat ggccgacttt aagcacatcc gcctgcaggt tatcgtgacg
     1981 gctgagcatg tctttttccg aatctttgga cgcgtctccc gtaaataaaa acgaaagctt
     2041 atggtaagta aaccgaatga cgatggaatc gttgttcata tcctcatagg ctgcgttcgg
     2101 agcatcaacc gtaaagcttc cgcccccgaa tttcgtcgtc tttccaacag ccggtttggt
     2161 aatcttcttt ccttttgact gaatggtctt gagaagattg taataaacat tggtgttgtt
     2221 cgccgttccg gtcatcataa tttccttgac gttaaaatcg gaaataattt gagaaagccc
     2281 gccgatatgg tcggaatgcg gatgggttcc caccacataa tcgagactgg taacccccag
     2341 ccttttgaga ttgttctcca ctgtgtcgga atcatcggga ttaccggcgt cgatcagcat
     2401 ggtcgccccg tcacaggaaa caaaaatgct gtccgcctgc cccacgtcaa agaaacaaac
     2461 ctcaagattt tcaattttgg ccggctgatc gggaaaaatc aaatggccgg ttgcgctgtt
     2521 cgctgtgttg accggattct gaagccagcc cggaaagaca ttgcggtcgc ccagattggc
     2581 aaacaggaca aacgccagaa cggcgacggc ggcaatgatt ccaagtggag tcagacgttt
     2641 cttttgcctt gccacgcgcc caacgcccct ctcttttttt tgcctttcgg atgaccgccg
     2701 cgtttttcgg ccggcctgat ctgcggcttg cgcacattcg tctgcgccct cgcatccggc
     2761 cggacaagac attcaccaga aaaaccgatc aggtcggtac gtcccgcctg tcggagcgca
     2821 tccagaacaa tcctccggtt ttccggttta aaatactgga gcagcgccct ctgcatcgcc
     2881 ttctcgccgg gagtgcgggg aacatagact tttttcatcg taaacgggtc aagacctgtg
     2941 taaaacatgc aggtcgagac cgtcccgggc gtcgggtaaa aatcctgcac ctgttcggga
     3001 cggatatgtt cctttttgag aaacagagcg agttccactg cctctttaag cgtactgccc
     3061 ggatgggacg acatcagata gggcacgaga tactgctccc gtcccgcttt cttggtaata
     3121 tcgtaaaact ttttcatgaa tttcacatag gtctcaatat ggggcttccc catccggtca
     3181 agcaccgcgg ccgaacaatg ctccggcgcg accttcagct ggccgctcac atgatgtttg
     3241 accagttccc ggaagaagct gtcgtcaggg tcctccatca tgtaatcgta gcgcagcccg
     3301 gaccggacaa aaactttttt aaccccggga agcgatctca gactgcgcag caggtcgagg
     3361 tattcccggt ggtcgacttc gagatttttg caaggcaccg gggcaaggca ctttttccct
     3421 ttgcacaaac cgaaagccag ctgccttttg caggacggtt ttcgaaaatt cgcggttggc
     3481 ccgcccacgt catggatata acctttaaag ttgggttttt cgatcagttt ctgcgcttct
     3541 tccaaaaccg actcccggct gcgcgacgta atcattcttc cctgatggaa cgatatcgcg
     3601 caaaaattgc aggctccgaa acagccccgg ttgtgcgtaa tggaaaactc aacctcctga
     3661 atagcgggaa ctcccccctg ggaatcgtaa accggatgcc atgcccgcat ataaggcagg
     3721 gaataaaccc ggtcgagttc ctgcgtattc agcggcaaag cgggaggatt ttggatcagg
     3781 acgacgcccc cgtgatcctg ggcgactgtt ttaccggtca cggcatcctg ctgttcatac
     3841 tgaatcctgc agcttttggc ataggctgcc ttgtcggatg agacccgttc acaggacgga
     3901 caaatcgccg tattttcggc aggaatgttt ttcgagagat aacaggtgcc ccggatgtcg
     3961 gtcaggccgg agatgttttc ccctctccgg agcctattgg aaagctcggc tgtctgccgt
     4021 tcccccatgc cgtaaaccag aaggtcggct cccgattcca tcaatatgga gggcctgacg
     4081 cggtcgtccc agtaatcata atgggcaaaa cgcctcaggc ttgcctcaag cccgccgatg
     4141 atcaccggca catcggggta agcctcctta atcttttggg tataaaccgt gaccgtccgg
     4201 tcaggcctgc agtgcggctt tccgccgggc gaataaaaat cctccgccct cggctttttg
     4261 gccgcggtat aatgattcac cattgaatcg atgttgccgg aggaaaccag aaatccgtat
     4321 cttggcctcc cgagcttttg aaaaccatcg gttgtcttcc aatcaggctg ggcaatgata
     4381 cccaccttaa agccctccga ctccagcacc cttgaaataa tggccgggcc aaaactgggg
     4441 tggtcgacat aggcgtctcc ggttacacag atgaaatcaa gataatacca cccgcgctca
     4501 gccatatcct ctttgcttac cggtaaaaat cccgacatgg ttcctccaat ctattctttc
     4561 ccgtcttcat tcatggtcat ttcgatccac tgctgacggg tgatgagcac ctgacgcggt
     4621 ttgctgcctt caaagggccc gacgatgccc ctcgcttcca tttcatccac aatgcgcgcg
     4681 gctctggcat aacccagctt gagccttcgc tgcaaaaagg aggtggaagc cattcccgcc
     4741 tcgatgacac attcaatggc ctgcggaagc attgcgtctg cgtcaccgcc gcggctatcc
     4801 tcgccgccgg acttatcctt ggcggactgc ttgtcaattt catccataat gtcttcactg
     4861 taattggagt cggctccgtt tttaatatat tctatgaccg attccacttc gttgtccgat
     4921 acaaaacagc cctgaacccg caccggtttg gatgctccga caggcagaaa cagcatgtcc
     4981 cccctgccga gcagcttttc cgcgccgccc atgtccagta tggtgcgtga atccacc
//
LOCUS       DATALY010000354         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_334813,
            whole genome shotgun sequence.
ACCESSION   DATALY010000354 DATALY010000000
VERSION     DATALY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334813"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VHR42_09725"
     CDS             complement(<1..659)
                     /locus_tag="VHR42_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027482.1"
                     /translation="MNRTLKCIRFEFGHLCKEAGIFTAIFFAAYLAIILLVYCTLSAT
                     GTSTTNMNFSFAAVIFSFVLMASSYKGHYNNLMMFGNTRSNILFSFFLKNLILSAFFG
                     VISILSDYFNLLCSQIFHFQTFAFLKMIYENKPGSALQLVWFFTLFTLIGSVGLLYGA
                     LAYKFGRQFKRWFWIGFGLFWVLTPLFTFAGFRSTVYHFFLFFFGLRQGAGILHATLH
                     F"
     gene            complement(656..1546)
                     /locus_tag="VHR42_09730"
     CDS             complement(656..1546)
                     /locus_tag="VHR42_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027483.1"
                     /translation="MNNLILETKDLTKKYGQVTAVDHVNLKLCQNKIYGLLGRNGAGK
                     TTFLNLITSRIFAESGEASLFGKPVTENAETLEKICYMPEKNLFLNRVKVRDTLRGAE
                     IFFKNYDPAYARALCEKFELNPDKKYNELSRGYESILRIVIGLASRSELTIFDEPVLG
                     LDAAVRDIFYRELIEDFTEHPRTIIISTHLIEESADIFDEAVIIQKGKLIEQAPVEAL
                     KERAHYISGKSDLVDRAAAGLNRIHTEEIAGVKICAVYQEIDSRKALEITQAGLELSP
                     IPVQKLFIYLTDHNGREAKS"
     gene            complement(1539..1913)
                     /locus_tag="VHR42_09735"
     CDS             complement(1539..1913)
                     /locus_tag="VHR42_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3027484.1"
                     /translation="MRLDFSNEKSIYLQIAENIEDDILKGAIAEEAQIPSTNQMAVLF
                     KINPATAGKGINLLVEEGILYKKRGIGMFVAAGAKKQIAQKRKSGFYDNYIAAMLEEA
                     KNIGITRNEIIEMISREAAAHE"
BASE COUNT          625 a          492 c          517 g          605 t
ORIGIN      
        1 aagaaatgaa gcgtagcgtg cagaatgccg gcgccttgac gaagaccgaa gaaaaaaagg
       61 aaaaaatggt agactgtgct tcggaatccg gcaaatgtaa acagcggagt gagtacccag
      121 aataatccga agccgatcca aaaccaccgc ttgaattgcc tgccaaattt ataggcgagc
      181 gcaccgtaaa gcaggcccac cgagccgatc agagtaaaaa gggtaaaaaa ccagaccagc
      241 tggagcgcgg agccgggttt attctcataa atcattttta aaaacgcgaa agtttgaaaa
      301 tgaaagatct gtgagcacaa aagattgaaa tagtccgaca aaatggaaat cacaccgaag
      361 aaagcggaaa gtataaggtt tttcagaaaa aatgaaaaga gaatgtttga ccttgtgttg
      421 ccgaacatca tcagattgtt ataatggcct ttatagcttg acgccatcag gacaaaagaa
      481 aagatcacag ccgcgaagct gaaattcata ttggtcgtgc tggtgccggt agcggacaag
      541 gtacaataaa caagtaggat aatcgcgagg taagcggcaa aaaatattgc ggtgaaaatt
      601 ccggcttctt tgcaaagatg tccgaattca aatcgaatgc actttaatgt tctgttcatg
      661 acttggcctc ccgcccgttg tgatcggtta gataaataaa cagtttttgc acaggaatcg
      721 gcgaaagttc cagaccggcc tgcgtgattt ccagagcctt gcggctgtcg atctcctgat
      781 aaacggcgca gattttaacg ccggcgattt cttcggtatg gatcctgttc agccccgcgg
      841 cagcgcggtc aaccagatcg ctttttccag aaatataatg agcgcgttcc ttgagtgctt
      901 ccaccggagc ctgctcaatc agcttgcctt tctgaatgat aaccgcctcg tcgaagatgt
      961 cggccgactc ctcgatcaga tgggtcgaga tgataatcgt ccggggatgc tccgtgaagt
     1021 cttcgattag ttcccggtaa aagatatccc gaaccgccgc gtcaagaccg agcaccggtt
     1081 catcgaaaat tgtcagttcc gatcgggagg caaggccgat cacaatacgg agaattgatt
     1141 cgtatccgcg ggagagctca ttgtatttct tgtccggatt gagctcgaat ttctcacaga
     1201 gagcccgcgc ataagccggg tcgtagtttt tgaaaaagat ttctgcgccg cgcagcgtgt
     1261 ccctgacttt gactctgttt aagaaaaggt ttttttccgg catatagcaa atcttttcca
     1321 gagtttctgc attttccgtt accggtttgc caaataggct ggcttcaccg ctttcggcga
     1381 aaatccgcga agtgattagg ttcaggaagg tagtttttcc agccccgttc ctgcccagca
     1441 ggccgtagat tttattctga cagagtttga ggttcacatg gtcgactgcg gtgacctgac
     1501 cgtatttttt tgtcaggtct tttgtctcga ggatgaggtt attcatgggc ggctgcctcc
     1561 ctgctgatca tttcaatgat ttcgttgcgg gtaatgccga tgtttttcgc ttcttcaagc
     1621 atcgcggcga tatagttatc gtaaaagccg cttttccttt tttgagcgat ttgttttttt
     1681 gctccggccg ctacgaacat gccaattccc cttttcttat aaagaatgcc ttcctccaca
     1741 agtaaattaa ttccttttcc agccgtagcg ggattgattt taaacagaac ggccatctga
     1801 ttggttgaag gaatttgcgc ttcttccgca attgcgcctt tgagaatgtc atcttcaatg
     1861 ttctcggcaa tttgcagata gatcgatttt tcgttactga aatccagcct cattatttca
     1921 cccggttctt ttcttaagta gttaattact tatgtaacta actatacagg ttttttacgc
     1981 gatgtcaaga ctttaccgaa aattcgttca gaaacagatc agctcttccg acaaaaaact
     2041 gtttacaaaa aaaattgcaa cttttttccc tgtctggtat aatcatggag gatacggaaa
     2101 cgatagaaaa aatgaacaaa aatcgagtca aaagttctca caatacttcc aaaaaaacat
     2161 gttgcaaagc caatcataat atgatattat ataaaagcgc gctgaacgtg cgcagatatg
     2221 cgatgaagcg ggaggttgc
//
LOCUS       DATALY010000355         5005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_334827,
            whole genome shotgun sequence.
ACCESSION   DATALY010000355 DATALY010000000
VERSION     DATALY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334827"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..342
                     /locus_tag="VHR42_09740"
     CDS             31..342
                     /locus_tag="VHR42_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027485.1"
                     /translation="MQDLVKQIVEMDRKARQITDAAQMEKVSSEKEIAERREQIRNDY
                     IARARKRISINEPNERAAAEADWEEKKKKYAELSRKLDELYSQKGDEWVKEIVARVIG
                     E"
     gene            348..1406
                     /locus_tag="VHR42_09745"
     CDS             348..1406
                     /locus_tag="VHR42_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX3027486.1"
                     /translation="MLSTLSSNVIIAKALAMYGHRLTDEDYRNLMACQTVGNVASYLK
                     NHTVYGKTLAGINENNIHRGQLEARLKQKLFEDYALLCRYEISIGEHFSRYLIVRSEI
                     EQILQSIIFLNSGKPEEYLFDMPDYLNQHTHINLAALTLIKDFDGLLDALEHTPYQKI
                     LEPFAPVQGDKIDYMGIENKLYTYLFSNAFEVIDKYTHGETAKQLREIFNSYVDLLNY
                     ARIVRLKVFYSAEPEFIRGSLLPFGDVSPRILEEMAAAENEEQVTNVMGRTSAGKRFL
                     NVPHTYIDEIPNRAKYKVCRHDIHFSTHSSVVLMSYVFITQAEITDIITIIESIRYKL
                     PPEEISKLLTVYNFNRKE"
     gene            1414..3360
                     /locus_tag="VHR42_09750"
     CDS             1414..3360
                     /locus_tag="VHR42_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEX3027487.1"
                     /translation="MAVLKVEVVSIIGRMADLDKVTALCGRSCVFHPDNALSFYSDTA
                     DFTPLNEENPYLQPLQQLTASLGGAKKKLELLSADEVEKINFSPDDFHSYVETITTSF
                     NDLLKQRADAQEKIQYYTQQIQDFSHFIGLNLNLDEIQECEYIKVRFGSLPKESYSKL
                     ASYKENPYIVFFPCTSDDTHFWGVYFSPIDMVSEVDRIFASLYFEKTQLTEMTGSPEL
                     AVEGLRSKRDEEISKIQRVDEKFDELWKNSKEVCQKLYSWLTEQSVYFGIRRYAARYN
                     ESFILTGWIPAEKESLIKAELDKIESVEYSFEGAEEEKTHSPPVKLKNKALFKPFEFF
                     IDMYGLPAYDEIDPTPFVAITYILLFGIMFGDLGQGICVSIVGWYMWNKMKMKLGRIL
                     IPCGISSAIFGTVFGSVFGFENALNPLYKLLLGMDEKPIEVMKPATTNMIIYSAVGIG
                     VLLVIVAILINIYSSLRRKRYESALFGPNGVAGLIFYGSLIVGFGGQIVFGWKIVSPV
                     FILCFIILPLVSMLFREVLGKLAERRPDWKPESWGEYITQNFFEVFEFLLSFATNTMS
                     FLRVGAFVLVHAGMMMVVFTLAEMSSGVGYLLVVIIGNVFVMALEGLLVGIQVLRLEF
                     YEMFSRFFDGDGRPFDPVVVRAEQ"
     gene            3400..3849
                     /locus_tag="VHR42_09755"
     CDS             3400..3849
                     /locus_tag="VHR42_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HEX3027488.1"
                     /translation="MNSVLFVLPAAALVATVIIAIKAVKNGKKPSKAVAMQLMAFLAV
                     CVVTFAVPVIANATTTGKAAPAASTSETAAAAGGSAVGIGLIAAALATGLSGIGGGIA
                     VGAAAPAAIGATSEDPKAFGKALIFVALGEGVALYGLLISILILNKI"
     gene            3874..4182
                     /locus_tag="VHR42_09760"
     CDS             3874..4182
                     /locus_tag="VHR42_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEX3027489.1"
                     /translation="MRFYLISDNVDTQVGMRLAGIEGIVVHEAAEVQKALNEAMEMDD
                     IAVILITETLLALCSDMVYDLKLNRKRPLIVEIPDRHGNGRTKDSITRYVREAIGVKI
                     "
     gene            4215..4811
                     /locus_tag="VHR42_09765"
     CDS             4215..4811
                     /locus_tag="VHR42_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HEX3027490.1"
                     /translation="MVTNDEKMNKFYLAINHYAEEQRKKIEQEVQEFKRRELDEAEVE
                     ALTEAYRLIQKEMAGMRNSIFHEMAHREMDCRRELLAKRQKISDDVFARCREKLTEFC
                     TKDEYAMLLEKSARELKKTFDKPGTVIRIKDGDRKYEELIRKAFGGDCTLEADASIRI
                     GGIRAYNSEIGIMADETLDSLLEDQHEWFEENSGMAVV"
     gene            4839..>5005
                     /locus_tag="VHR42_09770"
     CDS             4839..>5005
                     /locus_tag="VHR42_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027491.1"
                     /translation="MDNQNVIYGINGPVVTVKGTRSFSMMEMVYVGNERLVGEIINIS
                     DAFTTIQVYEET"
BASE COUNT         1349 a         1206 c         1282 g         1168 t
ORIGIN      
        1 cattcgaatt atcaaggagg tgcagtcaat atgcaggatt tagtaaagca gatcgttgaa
       61 atggatcgaa aagcaagaca gatcaccgac gctgcccaga tggaaaaggt cagttccgaa
      121 aaggaaatcg cggagaggcg cgaacaaatc cgcaacgact atatcgcccg cgcaagaaag
      181 cggatcagca taaacgaacc caacgagcgt gctgccgccg aagccgactg ggaagaaaaa
      241 aagaaaaagt acgctgaact ttcacgcaag cttgacgagc tttacagcca aaaaggcgac
      301 gaatgggtaa aagaaattgt cgccagagtt ataggggagt gatgtcaatg ctttccaccc
      361 tgtcttccaa cgttatcatc gcgaaagccc tcgcaatgta tgggcatcgg ctgaccgacg
      421 aagactaccg gaaccttatg gcctgccaaa ccgtcgggaa tgtggcttcg tacctgaaaa
      481 accacaccgt gtacggaaag acgctggcgg gaataaacga aaacaatatt catcgcggtc
      541 agctggaagc aaggctgaaa cagaagctct ttgaagacta cgccctgctt tgccggtacg
      601 aaatttccat aggcgaacac ttttcaagat atctgatcgt acggagtgaa attgagcaga
      661 tcctgcagag tatcatattc ctgaattccg ggaagccgga ggaatacctg ttcgatatgc
      721 cggattatct caaccagcat acgcacatca atctggccgc gctgaccctg atcaaggact
      781 ttgacggcct gcttgacgcg ttggaacata cgccgtacca aaagattctt gaacccttcg
      841 ctcccgtgca gggagataaa attgattaca tgggcattga aaacaagctt tacacctatc
      901 tgttttccaa tgcttttgaa gtgattgaca aatatacgca cggagaaacc gcgaagcagc
      961 tccgggaaat cttcaattcc tatgtggatc tgctcaatta cgcgcgcatc gtgcgtctga
     1021 aggttttcta cagcgcagaa cctgaattca tccgcggatc cctccttccc ttcggcgacg
     1081 tgagccctcg gattctggag gaaatggctg ccgctgaaaa cgaggagcag gtcacgaacg
     1141 ttatgggaag gacctccgcc gggaaacgtt ttttaaatgt tccccacaca tatattgatg
     1201 aaatcccgaa ccgcgcaaaa tataaggtat gccgtcatga cattcatttc tcaacccact
     1261 cttccgtcgt tctgatgtca tacgtattta ttacccaggc ggaaattacg gacattatca
     1321 cgattatcga atcgatccgt tacaagcttc cgccggagga aatttcaaaa ttactcaccg
     1381 tctataattt taacagaaag gagtaatgga gtattggctg ttttaaaagt tgaagttgtc
     1441 agtattatag gaagaatggc cgacctggat aaggtcaccg cattatgcgg aaggtcctgt
     1501 gtgttccacc cggataatgc gctttccttc tattcggata cggcggattt caccccgctg
     1561 aacgaagaaa atccctatct ccagccgctg cagcagctca ccgcttcact gggcggagca
     1621 aagaaaaaac ttgagctttt aagcgcggac gaggtcgaaa aaatcaattt cagcccggat
     1681 gattttcact cctatgtgga aaccatcacc acctcgttca acgacctgct caagcagcgc
     1741 gcggacgcac aggaaaaaat acagtattat acccagcaaa tccaggattt cagccacttt
     1801 ataggattga acctgaacct tgacgaaatt caggaatgcg agtacatcaa ggtgcgcttc
     1861 ggctcgcttc cgaaggagag ctactcaaaa ctggcgtctt ataaggaaaa cccatacatc
     1921 gtgttcttcc cgtgcacaag cgacgacacg cacttttggg gagtctactt ctcgccgatt
     1981 gacatggtca gcgaggttga ccgcattttt gcaagcctgt attttgaaaa aacacagctg
     2041 acggaaatga ccggcagccc cgaactggcg gtagaagggc ttcgaagcaa acgcgatgaa
     2101 gaaatcagca aaatccagcg tgtagatgag aaatttgacg aattatggaa aaacagcaag
     2161 gaggtatgcc aaaagcttta ttcctggctt accgaacaat ccgtttattt cggtatccgc
     2221 cgctatgcgg cccgatacaa tgaaagcttt atcctgaccg gatggatccc ggcggaaaag
     2281 gaaagtctca ttaaagccga gctggataag atcgaatcgg tcgaatacag ctttgaaggc
     2341 gcggaggagg aaaaaacaca ttccccgccg gtcaagctga aaaacaaagc tttattcaag
     2401 ccctttgaat tttttattga tatgtacggc ctgcctgcct acgacgagat tgaccccacc
     2461 ccgttcgtcg ccatcacgta catactgctg ttcggcatta tgttcggcga cctcggtcag
     2521 ggaatctgtg tttccatagt cggatggtat atgtggaaca aaatgaagat gaaactcggc
     2581 agaatcctga ttccgtgcgg catctcttcc gcaatattcg gaacggtgtt cggttcggtg
     2641 ttcggatttg aaaacgcgct gaacccgctt tacaagctcc ttttgggtat ggatgaaaaa
     2701 ccgatcgaag taatgaagcc tgccacgacc aacatgatta tctattccgc agtcggaatc
     2761 ggcgttttac tggtgatcgt cgcaatactg atcaacattt attcctccct ccggcggaaa
     2821 cgctatgaaa gcgctctgtt cggcccgaac ggagttgccg gcctgatctt ctacggatcg
     2881 ctgatcgtcg gtttcggcgg acagatcgta ttcgggtgga aaattgtaag cccggtattc
     2941 attctgtgct ttattattct tcctctggtc tccatgctgt tccgggaggt tttggggaaa
     3001 ctggccgagc gcagacccga ctggaaaccc gaaagctggg gcgaatacat tacccagaat
     3061 ttctttgagg tattcgagtt tcttctcagc tttgcaacca acaccatgag cttcctgcgt
     3121 gtaggcgcgt tcgtgcttgt tcacgccggt atgatgatgg ttgtgtttac tctggccgag
     3181 atgagcagcg gcgtaggtta cctgctggtc gtaattatcg gcaacgtgtt tgttatggca
     3241 ctggaaggcc tgctggtcgg tatccaggtt ctcagacttg agttctatga aatgttcagc
     3301 cgcttcttcg acggcgacgg ccgtccgttt gacccggttg ttgttcgtgc cgagcagtaa
     3361 agcttgtatt aaatattaaa aatgttggag gtaaatatta tgaattccgt attatttgtg
     3421 ttacccgccg cagcccttgt tgctactgtg attattgcca ttaaggccgt taagaacggc
     3481 aaaaagccaa gcaaagcagt tgctatgcag ctaatggcgt ttcttgccgt atgcgtcgta
     3541 accttcgcgg ttcctgtcat tgcaaacgcg accacaacgg gcaaagccgc ccccgcggcc
     3601 tcaaccagtg agactgcagc cgctgccggc ggctccgcag taggcatcgg cctgatcgcc
     3661 gcggctcttg caaccggtct ttccggcatc ggcggcggta tcgcggtcgg cgcggctgct
     3721 cctgccgcta tcggtgcaac aagcgaagac cccaaggcct tcggtaaggc gctgatcttc
     3781 gtagctctgg gcgaaggcgt cgccctttac ggtttgctga tttccatcct gatcctgaac
     3841 aagatttagt taccgaaagc cgaggtgcag agaatgcgct tttatctgat tagcgataat
     3901 gtggacacgc aggttggcat gaggcttgcg gggattgagg gaatcgtggt ccatgaagcc
     3961 gcggaagtgc agaaagcact gaacgaggcc atggaaatgg acgacatagc cgttattctg
     4021 attacggaaa ccctgcttgc actttgttcc gacatggttt atgacttaaa gctgaaccgg
     4081 aagcgcccgc ttattgttga aattccggac cggcacggca acggccgtac gaaagattcc
     4141 attacccgct atgtccgcga ggcgatcggg gtcaaaatat aggccgcttg ttacggcgaa
     4201 acgggggtta atagatggtt accaacgatg aaaaaatgaa taaattttac cttgccataa
     4261 atcattacgc cgaagaacag cgcaaaaaga tcgagcagga agtgcaggag ttcaagcgca
     4321 gagaactgga cgaagccgag gtggaagctc tgaccgaggc ctaccggctt atccagaagg
     4381 aaatggcagg aatgcgcaac agtattttcc acgagatggc gcaccgcgaa atggactgcc
     4441 gcagagaact tcttgcaaaa cggcagaaaa tatccgacga cgtctttgcg cgctgcaggg
     4501 aaaaactgac cgaattctgc acaaaggacg aatacgccat gctgctggag aaatccgcac
     4561 gggaactgaa gaaaaccttt gacaagcccg gcacggtcat ccgcataaag gacggcgacc
     4621 gcaagtatga ggaactgatc agaaaagcct ttggcggcga ctgcacttta gaggctgacg
     4681 cttccatccg aatcggcgga atccgggctt acaattccga aatcggcatt atggcggatg
     4741 aaaccttgga ctccctattg gaagaccagc acgaatggtt tgaagaaaac tcgggaatgg
     4801 ctgtggtcta agcctatcct ttttggagat gatgaagtat ggataatcag aatgtgatat
     4861 atggcatcaa cggccctgtt gttaccgtga agggaacccg aagcttttcc atgatggaaa
     4921 tggtatatgt agggaatgag cgccttgtag gcgaaattat caacataagc gacgcgttca
     4981 ctactattca ggtttacgag gaaac
//
LOCUS       DATALY010000356         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_77476,
            whole genome shotgun sequence.
ACCESSION   DATALY010000356 DATALY010000000
VERSION     DATALY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77476"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VHR42_09775"
     CDS             <1..675
                     /locus_tag="VHR42_09775"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3027492.1"
                     /translation="SKIPSNVKEGFETSREGLNQIHRYISEIMPGLPMKLVIRSFGAI
                     RPNPFYVKYDQQSGSYQPDQKKINDFSVSQSEENPAFLSLIGIKTPGLTCSEALGRHV
                     ADRMASLLGAERNPEFDPKRPAPLRLNDIPFEERAGIVGKNPAYGRIVCRCRGISEGE
                     IIDSIHMAPGAVTVDGVKRRTGACSGRCQGSFCTQRIVQILARELKVPTGRINKDGPG
                     SYLIGG"
     gene            680..1753
                     /locus_tag="VHR42_09780"
     CDS             680..1753
                     /locus_tag="VHR42_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3027493.1"
                     /translation="MNSYDLVVIGGGPAGMAASLAARQAGVDGILLAERDDRLGGILN
                     QCVHSGFGLIYFGEELTGQQYAERFVRRITGSGVSIYTGTTAVSLSDGIVLLSGEKCG
                     LLQAEAKAVILASGCRERPVGTLPVVGTRPSGIFTAGMAQKMTNLGGYDIGSRFVILG
                     SGDIGLIMARQLRLAGKEVLAILEKEGKCGGLERNRVGCIEKYGIPLRTRCTVSKIHG
                     KGRITGVTVQNFTDGTESDIDCDTLITSIGLIPERELAEEASGGGKLPEWLFLCGNSC
                     YVHDLVDDVTYEAEEIGRQAAKYILSGEARNNIKTAFDKSKVKDSGIFCIACPKACAL
                     TKTENGYAGAVCGRQDPIPSKKL"
     gene            1928..>2983
                     /locus_tag="VHR42_09785"
     CDS             1928..>2983
                     /locus_tag="VHR42_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027494.1"
                     /translation="MKKIIAVLSAAAILCLPGCVSKANGGSAKSQPVYGISSVPSAFI
                     MSPQSESTAISSESSVGSAAAYAADGKIAVSSQSLKSSVPKFSDSSKISSSAAKQKEI
                     VASSSAKSNSAGAGSQTIVSAISRVVSSTASQMTQKPAGDVKILFVGNSFFYVPDTPS
                     QFSQILLNKGINAEIMQKTGGAYTLHHHLMDFKTGKYNKLIEKANIVILQEHFDQPDT
                     VESIKEIGALFKPGTIFYLTITDSDKCQYRIDQLKAAGNIKPIASPLQKMLDNGFAYA
                     KLATGDGHMNRFYGYIEACTIFSTIYSCPIENLPYDQINKETVKYIPGTDKQKSVKTA
                     LKSVTEAITVVNSLWFKA"
BASE COUNT          821 a          692 c          777 g          693 t
ORIGIN      
        1 tcgaaaattc cctccaacgt aaaagaagga tttgagacat cacgggaggg tctgaatcaa
       61 attcatcggt atatttccga aataatgccg ggtttaccga tgaagcttgt catccggtca
      121 ttcggcgcga tcaggcccaa cccgttttat gtgaaatatg atcagcaaag cggctcctat
      181 cagcccgacc agaagaaaat aaacgatttt tcagtcagcc agtcggagga aaatccggct
      241 tttctgagcc tgatcggcat aaaaacgccg gggctgacct gctcggaagc gcttggccga
      301 catgttgccg accggatggc ttctttactg ggagcggaga gaaacccgga gtttgacccg
      361 aagcgtccgg ctcctctgag gctgaatgac attccgtttg aggaacgcgc ggggattgta
      421 gggaaaaatc cggcttatgg cagaatcgtc tgccggtgcc gcgggatatc cgagggagaa
      481 ataatagaca gtattcatat ggccccgggc gccgtaacgg tcgacggcgt aaagcgccgg
      541 acaggcgctt gctcggggcg atgccagggc agcttctgta cgcaaaggat cgtgcaaatt
      601 ctcgcccgcg agctgaaagt cccaaccggc agaatcaaca aggatggccc cggttcctat
      661 ctgattgggg gatgaagtga tgaactccta tgacctcgtc gtcatcggcg gcgggccggc
      721 aggcatggct gcttcgcttg ccgcgcggca ggcgggcgtt gacggcattc ttctggccga
      781 acgcgacgac aggctcggcg gtattttaaa ccaatgcgtc cattccggtt tcggccttat
      841 ttatttcggt gaggaattaa ccggtcagca gtatgccgag cgttttgtac gcagaataac
      901 cggctccggc gtcagcattt atacaggcac aaccgccgtc agccttagtg acggcatcgt
      961 ccttttaagc ggagaaaaat gcggcctgct tcaagcagaa gcaaaggccg taattcttgc
     1021 atccgggtgc agggagagac cggtcgggac gctcccagtc gtcgggacgc ggccttccgg
     1081 aatatttacg gcagggatgg cgcagaaaat gacaaacctc ggcggatacg acatcggcag
     1141 ccggtttgtc attctcggtt caggggatat tggcctgatc atggcgcgtc agctcaggct
     1201 tgcaggcaaa gaggttttgg cgattcttga aaaagagggg aaatgcggcg gccttgaacg
     1261 aaaccgggtc ggctgtattg aaaaatacgg catacccctg cggactcgct gcacggtcag
     1321 caaaatccac ggaaagggcc ggattacggg cgtgaccgta caaaatttca cggatggaac
     1381 ggaaagtgat atcgactgcg atactctgat tacctcgatc gggctcatcc ccgaacggga
     1441 gctggcggag gaggcatccg gcggtggaaa gcttccagaa tggctgttcc tttgcggaaa
     1501 ttcctgctat gttcatgatt tggtggacga tgtaacttat gaagcggaag aaatcgggcg
     1561 gcaggcggca aagtatatat tgagcggtga agcaagaaat aatatcaaaa cagcgtttga
     1621 caagtcaaaa gtaaaggact ctggaatatt ctgtattgcc tgtcccaaag cctgcgcgct
     1681 gacaaaaacg gaaaacgggt atgccggcgc tgtctgcggc cgacaagacc ccattccgtc
     1741 taaaaaactc tgaggccctc taacgccagg caaattcagt ggatgcaatg cagctatctc
     1801 caatattttc agtctctgct ttaaaactta aaaggtaaac gctgataaca ttataagttt
     1861 cgtatattaa tccgcacata tagttatgtt aaaacgcttg attctgaaat actaaacact
     1921 ttgttagatg aaaaaaataa ttgctgtttt gtcagccgct gctattctat gcttgcccgg
     1981 atgcgtttca aaagcaaacg gaggctctgc caaaagccag ccggtatatg gcatatcatc
     2041 ggttccttcc gcgtttatta tgtctccgca atcggaaagc actgcgatat cgtccgagtc
     2101 atccgttggt tcagcggctg catatgccgc tgacggtaaa atcgctgtat ccagccaaag
     2161 cctgaaaagc agcgtgccga agttttcaga cagcagtaaa atcagttctt cggctgctaa
     2221 gcaaaaagaa attgtagcaa gttcatcagc aaaaagcaat tcagccggag ccggttcaca
     2281 gaccatcgta tcagccatat ccagagtggt ttcctccact gcgtcccaga tgacgcagaa
     2341 gcctgccggt gatgtgaaaa tactgtttgt gggaaatagt tttttctatg tgccggatac
     2401 gccgtctcag ttttctcaaa ttttattgaa caagggaata aacgcagaaa ttatgcagaa
     2461 aaccggcggc gcttatactc tgcaccatca tctgatggat tttaaaacag gtaaatacaa
     2521 taaattgatt gagaaagcaa atattgtaat actgcaggaa cattttgatc agcctgatac
     2581 tgttgaatca ataaaagaga taggggctct atttaaaccg ggtaccatat tttatctgac
     2641 tataacggac agcgataaat gtcaatacag gatcgatcaa ttgaaggcag cgggcaatat
     2701 taaacctata gcaagcccgc tgcaaaaaat gctggacaac ggttttgctt atgcaaagct
     2761 tgctacgggc gacggccaca tgaaccggtt ctatggttat attgaggcat gtacgatctt
     2821 cagcacgatt tattcctgcc cgattgagaa tcttccatac gatcaaatca acaaggaaac
     2881 tgttaaatac atacccggaa cggataaaca aaaatccgta aaaaccgcgc tgaaaagcgt
     2941 tacagaggca ataactgtag ttaattcatt atggttcaag gcg
//
LOCUS       DATALY010000357         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_421668,
            whole genome shotgun sequence.
ACCESSION   DATALY010000357 DATALY010000000
VERSION     DATALY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421668"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VHR42_09790"
     CDS             <1..615
                     /locus_tag="VHR42_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX3027495.1"
                     /translation="DEEPDSTYQTMCKIYEEQFHCSTDQLTKEDLRKFVKAAVKIKTA
                     DIIYVGDGNALRLMSLLRKYKIDVLLRDAFHSRTVLCGVNAGAICWCSCGNSDSRKFT
                     SGSDQLIRVNGLGLIPLLLCPHYDVEPARQADLKRMMKSTHNIPAIAMDKGTALEIKD
                     GCYRILRSLENAEAKKCYWKSGEYRMEVLPIQDQFEKVAPLFAK"
     gene            complement(747..1223)
                     /locus_tag="VHR42_09795"
     CDS             complement(747..1223)
                     /locus_tag="VHR42_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3027496.1"
                     /translation="MELTQDQKCLFHDCTEDLFASANVQRMGTFVQHADVSCLEHCIS
                     VALMSLWVSEKLGLSVDRRSMVRGALLHDFFLYDWHEKSDRKGLHGFTHSKTALRNAQ
                     LEFRLNDREKDIIVKHMWPLTLVPPRCREALIVSCSDKFCSLLETLRLYPKYGSIS"
     gene            complement(1293..1556)
                     /locus_tag="VHR42_09800"
     CDS             complement(1293..1556)
                     /locus_tag="VHR42_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX3027497.1"
                     /translation="MKSTGIVRKVDELGRIVLPKELRVVLNNNEKDPLEVFVDEENRI
                     ILKKYEPACIFCNGMNEIINYKGYNVCKDCREKLNEKLAETEK"
     gene            1736..>1992
                     /locus_tag="VHR42_09805"
     CDS             1736..>1992
                     /locus_tag="VHR42_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HEX3027498.1"
                     /translation="MNKLLNYIWAGLILVSILCAAATGRMPELSAAVLSGAGGAVTLI
                     ISMAGMMAAWTGLMKIADRSGATRLLAKLLGPLMHLLFPQC"
BASE COUNT          533 a          509 c          471 g          479 t
ORIGIN      
        1 gacgaggagc cagattccac gtatcaaacg atgtgcaaaa tctatgagga acaatttcac
       61 tgcagcacag accagctgac caaagaagac ctgcgcaaat ttgtaaaggc cgctgtaaaa
      121 atcaaaacgg ccgatattat ctatgtcggg gacggcaacg ccctccggct gatgtctctg
      181 cttcgaaaat acaaaattga cgttctgctg cgggatgcct ttcacagcag aaccgtgctg
      241 tgcggagtaa acgcaggagc catctgctgg tgcagctgcg gaaattccga ttcccgcaaa
      301 ttcaccagcg gaagcgacca gctcatccgg gttaacgggc ttgggctgat tccccttctc
      361 ctctgccctc attacgacgt cgaacccgcc cggcaggcgg atttaaagcg gatgatgaaa
      421 agcacacaca atattccggc tattgcgatg gacaagggaa ccgctctgga aattaaggac
      481 ggctgttacc gcattcttcg gagcctcgaa aacgctgaag caaagaaatg ctactggaaa
      541 agcggcgagt atcggatgga agtgctcccg attcaggacc agtttgagaa agtggctcct
      601 ctgttcgcaa aatagagaga atcggatcat aacccctaaa aaactgtgcg ccctgaaacg
      661 gccttttcgg ccggatcggg gcgcgcttca ttgtgcatat ttacagagaa ccttccgaaa
      721 ccggacggtc cttttttatc ccgctttcag gaaatggatc cgtacttcgg ataaaggcga
      781 agcgtttcga gaagcgaaca gaatttatcg gaacagctga caatcagcgc ttccctgcat
      841 cggggcggta caagagtcaa cggccacatg tgcttcacga taatatcctt ttcccggtca
      901 ttgagccgga attccaactg cgcgttgcga agcgccgtct tcgaatgagt aaaaccatga
      961 agacctttcc ggtcgctctt ttcgtgccag tcatacaaaa aaaaatcatg cagaagcgca
     1021 ccgcgtacca tgcttcgccg gtctacgctg agaccgagct tttcgctgac ccaaaggctc
     1081 atgagcgcaa cggaaatgca atgctccagg caggaaacgt cagcgtgctg aacaaaggtc
     1141 cccattcgct gaacattagc agacgcgaaa agatcctccg tacagtcgtg gaaaaggcat
     1201 ttctgatcct gcgtcagttc cacccgactt cacctctctg caaaccaagt ctatggtgca
     1261 ggaagaaaac ctgtctgaaa aagcgaactc tattattttt cggtttccgc cagtttttcg
     1321 ttgagttttt ccctgcagtc cttgcaaaca ttataccctt tataattgat aatttcattc
     1381 attccattgc aaaaaataca ggcgggctca tacttcttca gaataattcg gttttcttcg
     1441 tccacaaaga cctccaaggg atccttctcg ttgttgttca gaaccaccct gagctccttc
     1501 ggcaacacaa tccgaccaag ctcgtcaact ttgcgaacaa ttccggtaga tttcaaatgt
     1561 aacgacatcc tttcgctaaa acatctttga tttattatga tcagacaaat tataggatat
     1621 ttttccattt ttgtaaacat atgtagagaa caaaatgaaa aaattttttg gaaaattata
     1681 ggatagccat aatcggcttt gaataattat tatacaagga attttcagca gggagatgaa
     1741 caaactgtta aattacattt gggcggggct gatcctggta agcatccttt gtgccgcggc
     1801 aacgggccgg atgccggagc tttccgcagc cgtgctgagc ggtgcgggcg gcgcggtcac
     1861 cctgatcatc tccatggcgg gaatgatggc cgcatggacc gggctgatga aaatcgccga
     1921 ccgctccggc gcgacgcggc tgcttgcaaa gcttctcggc ccgctgatgc atctgctttt
     1981 ccctcagtgc aa
//
LOCUS       DATALY010000358         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_421950,
            whole genome shotgun sequence.
ACCESSION   DATALY010000358 DATALY010000000
VERSION     DATALY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421950"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VHR42_09810"
     CDS             <1..672
                     /locus_tag="VHR42_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001969037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEX3027499.1"
                     /translation="GIQGIQGVQGVQGIQGVQGIQGIQGPTGPTGPTGPTGPTGLTGP
                     TGPTGPTGPTGPQGIQGIQGIQGVQGIQGIQGPIGPTGATGATGATGATGTAVSDAFG
                     YYFSTTPGVVGVNSDVPLNAFTEQRNMALVGSGVIQVSVAGVYKIDFSVNATSSPFST
                     IQLFINNAAVGGTQTTAAATVGQLEGEVIVTLGANSTVELRVTGVGLALGSGVNAFIA
                     LVKIA"
     gene            745..1818
                     /locus_tag="VHR42_09815"
     CDS             745..1818
                     /locus_tag="VHR42_09815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3027500.1"
                     /translation="MITISLCMIVKNEEQVLERCLRSCGNLFDEIVIVDTGSTDTTKQ
                     IASKFTSNIYDFEWVDDFSRARNFAFSMATGEYLMWLDADDVLMPEDLSKLRTLKKVL
                     SHNVDAVMMKYRIGLDQKGRASMTYYRERLVKRASGFRWLDPVHEYIPVTGNISSVDI
                     AVTHQKEKTEDPNRNLRIYQRMIANEEPFSSRNTFYYARELADHGMAAEAAEQYEKFL
                     GSSDGWVEDYISACYELSYCYEKLNNSHNMISALVESFRFSKPRAEICCRIGDYHRSR
                     ESYHEAAFWYELALSLEKPSGWGFVQEDYWGYIPAMQLCVCYYNLGDLKASERCNTLA
                     KSYKPDDPGVISNEKFFLGARKK"
     gene            1941..2888
                     /gene="prmA"
                     /locus_tag="VHR42_09820"
     CDS             1941..2888
                     /gene="prmA"
                     /locus_tag="VHR42_09820"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3027501.1"
                     /translation="MNYHKIIVYTTSEGIEPVCGRLYSLGVTGVEIEDEKDFNEFLKS
                     NQPYWDYVDEELCKKMSGETRVMAYLADDPTQEETFHEIAEALKELKEIDSESRFGRL
                     EMDVQSVSEDDWANNWKQYYKPTRIGKKLVVVPEWESYDARENDRIIKIKPGMAFGTG
                     THETTRLCMELIEKYVSEGTSMLDIGTGSGILAVAALILGAQSALGVDIDEIAVKVAM
                     ENACLNGVGERLSTVCGDLVGKVTGRYDLITANIVADVIIRLAPSVKQYLAKGGVFIA
                     SGIIDTREDEVTDALFAEGLTAFDRRTDRGWVALALRAN"
     gene            2987..3709
                     /locus_tag="VHR42_09825"
     CDS             2987..3709
                     /locus_tag="VHR42_09825"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX3027502.1"
                     /translation="MPRFFSEQVGDGSVRIEGQDAAHISKVLRMKTGDTLVVCDCKGS
                     DHFCKIIHLEQGVVKLAILRTEPSASEPSVSVTLYQALPKSDKMDLIVQKCVELGVKR
                     IVPVITSRCVSRPDGKAIVSKVERWNKIAVQAAKQSGRGILPAVEQAYGFRQALGKLA
                     SHESGILFYEQNGGRLSESLKGTEQDIGIFVGPEGGFEQEETDAAAQCGIAIAGLAPR
                     ILRTETAPLCALSAIMFATGNI"
BASE COUNT          979 a          834 c         1082 g          859 t
ORIGIN      
        1 ggaatccagg ggattcaagg tgtccaggga gttcagggaa tacagggagt ccaggggatc
       61 caaggaatcc agggcccaac gggcccaacg gggccaaccg gcccgacagg accaaccggc
      121 ctaacaggac ccaccggccc gacaggaccc acgggaccca ccggtcccca gggaatccag
      181 gggattcagg gtatccaggg agtccaagga attcagggaa tccagggtcc gataggcccg
      241 accggcgcaa caggcgcaac aggtgcaaca ggcgctaccg gaacagcagt ctccgatgca
      301 ttcggatatt atttctccac aacacccgga gtggttggtg tgaatagcga cgttcctctc
      361 aatgcgttca cggagcaaag aaatatggcg cttgtgggga gcggcgtaat tcaggtgtcg
      421 gtagccggag tgtataagat tgatttctcc gttaacgcca catcttctcc ctttagtaca
      481 atccagctgt ttatcaataa tgctgcggtg ggaggaacac agacgaccgc cgccgcgacg
      541 gttggccaac tggaaggaga ggttattgta acgctcggag caaacagtac cgtcgaactt
      601 cgggtaaccg gcgtaggcct ggcgcttggc agcggagtga atgcgtttat cgctcttgtt
      661 aaaatagcct gatcagcatg gcataggccc gggcgggcga aataatttgt ttcgcccgcc
      721 ttttttacgg aaaggaggga tattttgatt acgatcagcc tttgcatgat tgtcaaaaat
      781 gaagaacaag tgttggaaag atgtctgaga tcctgcggaa acctttttga tgagatcgtt
      841 attgtggata caggctcgac ggatacgaca aaacagattg cgtcaaaatt tacatcaaat
      901 atctatgact ttgagtgggt tgatgatttt tccagggcaa ggaattttgc attctccatg
      961 gctaccggtg aatatctcat gtggcttgac gccgacgatg ttcttatgcc ggaagatctt
     1021 tcgaagcttc ggacgctcaa gaaagttctg agccataatg ttgacgctgt gatgatgaaa
     1081 taccgtatcg ggctggatca aaaaggacgg gcgagcatga cctattacag ggagcgtctg
     1141 gtgaaaaggg ccagcggatt ccgctggctg gatcccgttc atgaatatat tccggtaaca
     1201 ggcaacatca gcagtgtgga tatcgccgtt acgcatcaga aggaaaagac agaagatccg
     1261 aaccgaaatc tcaggatata tcagcggatg atcgcaaacg aggagccgtt cagttcccgc
     1321 aatacctttt attacgcaag ggagcttgcc gatcacggaa tggcggctga agcggctgag
     1381 cagtatgaga aattccttgg cagttccgac ggctgggttg aggactatat cagcgcatgc
     1441 tatgagctgt cttactgcta tgaaaagctt aataacagcc acaacatgat ttccgcgctc
     1501 gtggaaagct ttcggttttc gaagcccaga gctgaaatct gctgccggat cggggattac
     1561 cacaggagca gggaaagtta ccatgaggcg gcattttggt atgaactcgc gttaagtctt
     1621 gagaagccgt ccggctgggg atttgttcag gaggattact ggggatatat tccggccatg
     1681 cagctgtgtg tttgctatta taatcttggc gatttaaaag cctcggagcg ctgcaacacc
     1741 cttgccaagt cttacaaacc ggatgacccg ggcgtgatca gcaacgagaa attcttttta
     1801 ggggccagaa aaaagtaact cgatttcttg gggccggcgg atgcagcaat gcatccgctt
     1861 tttcgtcttg cctgttttgt gcattcatat tataatggtc tggtaaaaca aaggtcgatg
     1921 ttcacaggac ggaggaaaaa atgaattatc ataaaatcat tgtctatacc acttccgagg
     1981 gaatcgagcc ggtttgcgga aggctttaca gccttggcgt caccggcgtt gagatagagg
     2041 atgaaaaaga ttttaacgag tttctcaaaa gcaatcagcc atattgggac tatgtcgacg
     2101 aagagctttg caaaaaaatg tccggtgaga cacgtgtgat ggcctatctc gcggatgacc
     2161 ctacccagga ggaaacgttc catgaaattg cagaagcgtt aaaagaatta aaggaaattg
     2221 atagcgaaag ccgtttcggc aggctcgaaa tggatgtgca gagcgtcagt gaggatgact
     2281 gggcaaacaa ctggaaacaa tattataagc ctacccggat cgggaaaaag ctggtcgtgg
     2341 ttccggagtg ggagtcttat gatgccaggg aaaacgacag gattatcaag ataaaacccg
     2401 gtatggcttt cgggacggga acgcacgaaa cgacaaggct ctgtatggaa ctgattgaaa
     2461 aatatgtctc agagggaacc tccatgctcg atattggaac agggagcgga attctggccg
     2521 tcgcggctct gatactcggc gcgcagtcgg cgctgggcgt ggatattgac gagattgccg
     2581 taaaagtcgc tatggagaat gcctgcctca atggggtcgg agagcgtctt tcgaccgtct
     2641 gtggggatct tgttgggaaa gtgacgggcc ggtatgatct gatcaccgcg aatattgtgg
     2701 cggatgttat catcaggctg gcgccttcgg tgaaacaata tcttgcaaag ggcggagtgt
     2761 ttatcgcatc cggcattatt gacacccgcg aagacgaggt gaccgatgct cttttcgccg
     2821 agggactcac tgcctttgat cgcagaacag accgtggatg ggtggcgctg gcgctgcggg
     2881 cgaactaaga cccgggccca tgacaggctc acacaccggc tcggacgtgc ctgcgcgtca
     2941 cagggtattt gacatgcaaa agaatttata gggaaggaat tcgaacatgc caagattttt
     3001 ttcggaacag gtcggcgatg gttccgttcg gattgaaggg caagatgccg cgcatatttc
     3061 aaaggtcctg aggatgaaaa ccggcgatac gcttgtggtc tgcgactgca aaggcagcga
     3121 tcatttttgc aaaatcatac atttggagca gggggttgtg aaactcgcaa tccttcgaac
     3181 cgagccgtcg gcttccgagc catctgtcag tgttacactt tatcaggcgc tgccaaaatc
     3241 cgataagatg gatttgatcg ttcaaaaatg tgtggagctt ggtgtaaaac gtattgtacc
     3301 ggtcattacg tcaagatgcg tttcccggcc ggatgggaag gcaattgtgt ccaaagtcga
     3361 gcgctggaat aaaattgccg tgcaggccgc gaagcaatcc ggccgaggca tattacccgc
     3421 cgttgagcag gcgtatggtt ttcgccaggc cttgggaaaa ctggcttctc acgaatcggg
     3481 aattttgttt tatgagcaaa acggcggcag gctttccgaa tcgctaaaag gaacggaaca
     3541 ggatattgga atttttgtcg ggccggaagg cggcttcgaa caggaggaaa cagacgcggc
     3601 ggcgcaatgc ggcatcgcga tcgccgggct tgccccccgg atattgagaa cggaaaccgc
     3661 gccgctttgc gctttgtcgg ctattatgtt cgcgacgggc aatatttaaa tattttataa
     3721 ggccccggca tttcactgaa acaaaatagt aaaa
//
LOCUS       DATALY010000359         8997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_683142,
            whole genome shotgun sequence.
ACCESSION   DATALY010000359 DATALY010000000
VERSION     DATALY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683142"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1715
                     /locus_tag="VHR42_09830"
     CDS             264..1715
                     /locus_tag="VHR42_09830"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX3027503.1"
                     /translation="MKLSQLMKDIADIDKKEDREIAGITCDSRRAAEGWAFVCIKGTA
                     VDGHRFAEKAEADGASVIIAEDDTGCKNQLMVPTTRKAWAKMCANWFGNPSDRLHLIG
                     VTGTNGKTSTTYMLKSVLEECGYKVGLVGTIQNMIGDRVLPSGHTTPDPYDLQSMFAL
                     MAAEGCTYAVMEVSSHALDQDRVGGCMFDAAVFTNLTQDHLDYHKTMENYLEAKKKLF
                     RMCRKAVVNKDDPWAGKLIEGLTCPMITYSTYQDTADYTAKNIRPRPDGVDFELVGTG
                     AIGRVRLPIPGMFSVYNAMAAATCALALGLPFGQLIDALSHVKGVKGRAEIVPTGRDF
                     TVVIDYAHTPDGIEKICKTMKQCSKGRLVTLFGCGGDRDKTKRPRMGAAAASLSDFLI
                     VTSDNPRTEEPGAIIQDILTGIPENGTPYTVIENRVQAIHWAIANARPGDTILLAGKG
                     HETYQILKEGRIHMDEREIVADALRETEQNSKL"
     gene            1740..2753
                     /gene="mraY"
                     /locus_tag="VHR42_09835"
     CDS             1740..2753
                     /gene="mraY"
                     /locus_tag="VHR42_09835"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX3027504.1"
                     /translation="MNIMMTIVSASALVAFLLTAVSGRAVIPLLHKLKFGQTIRDVGP
                     SWHKNKQGTPTMGGVMFIGATIVTFLLAIAACYFIVPAKVLNVTPLIKTRLYGGLLMA
                     TGCGIVGYADDYIKVVKKRNLGLTARQKMFGQLLVALAYSLSIYLIRGTDVYVPFVGN
                     VEFGIWFIPFCMFVIVSMTNAVNLTDGIDGLCSSVSFIVCLFFIVAAGVTGFSGQSIL
                     ASILAGALAGFLVWNLHPAKVMMGDTGSLFIGGLITALAFGINQPFLLIPVGIIYIVE
                     VLSVMMQVTYFKITHGKRLFKMSPIHHHCELSGWSENKIVTVFTAITLLGCAAAWVIL
                     IYA"
     gene            2878..4056
                     /gene="ftsW"
                     /locus_tag="VHR42_09840"
     CDS             2878..4056
                     /gene="ftsW"
                     /locus_tag="VHR42_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX3027505.1"
                     /translation="MAVSRSAAVSAAPPKTKKRLRFFSVAQGFDMPFLIILMIILVVG
                     LIMLFSAGYAYSYYWNNGDSFYYIKRQLIFAVLGVTAMLLISTVDYHVWHRFAIPLMI
                     ISYVLLGVVLLLPSSIGVYRWIRLPGLGQFQPSEIAKFSLILLFAHLISLNHKKMKTF
                     TSGYLPFIIILGVTCGLVFIEPHLSGTILLLAIGIVMMYVGGTRLFYLLGTLGLGFGA
                     VVYMIFVKGYEQDRIDVWLHPFEAYTTNKNEAWQTIQSLYAIGSGGVMGQGLGNSRQK
                     HLFLPEPHNDFIFSVICEELGFIGAVLVIVLFSLLVWRGITIGMKSPDKFGSLLAVGL
                     TVQIGLQAVMNMAVVTNTIPNTGISLPFFSYGGTSLIMLLAQMGVVLSISRNVNTRKT
                     "
     gene            4278..5405
                     /gene="murG"
                     /locus_tag="VHR42_09845"
     CDS             4278..5405
                     /gene="murG"
                     /locus_tag="VHR42_09845"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX3027506.1"
                     /translation="MRVLMTGGGTAGHINPALAIAAKIKQMNPEAEILFVGAKGRMET
                     KLVPAAGYKIVTVDVRGFQRRLSLKNVGRNLSAAVHVVTAGAACSRILKDFRPDIAIG
                     TGGYVSGPILRKAAKMGIPVLVHESNAYPGVTVKMLAKYAAATMIAEEAARKYLPGGA
                     NVIVTGNPLRNDFLHLDREAARRELNLDERPLVLSVGGSLGAAHINSAMAEVLARSAK
                     EGKLQHIHATGHAGYEEMKKMLKEKGIGENTPGIRVREYIDDMPRCMAAADLVISRCG
                     AMTLSELPAAGKPSILIPSPYVAENHQYHNAMALVRKGAAVCIEEKNLTGEALWKSIH
                     NITSSPDMMRSMSENARKTAILDATERIYKVIEDTLKKEKK"
     gene            5797..7056
                     /gene="murA"
                     /locus_tag="VHR42_09850"
     CDS             5797..7056
                     /gene="murA"
                     /locus_tag="VHR42_09850"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX3027507.1"
                     /translation="MSRLIIEGGHKLNGAVTIHGAKNGALPILAATLLAKGPSEIQNC
                     PALSDVAASVRILRYLGCMVNRSEGCVVVDSAGLNRCDIPDSLMREMRSSIVFLGAIA
                     ARTGEAHMSFPGGCELGTRPIDLHLSALRQLGMEIVEERGRLECRVNGRMKGATIALA
                     FPSVGATENILLASATARGTTTILNAAREPEIEDLCDYLVQCGAHISGAGDSAIIVEG
                     VDALHGCVHRVIPDRIETVTYMAAAAITGGSIVLQNIHPEHIATVLPAFEEAGCEVTI
                     GNGELYLCAPERLRRIRSVRTMPYPGFPTDAQAPAMAMTSVADGTSVFIENIFESRYK
                     HAGELMRLGAKIKIEGRVAVVEGIPRLSGAQVECTDLRGGAALVIAGLNAEGITDVTQ
                     LHHIDRGYEKLEESLRSVGAVVRRIDE"
     gene            complement(7156..7824)
                     /locus_tag="VHR42_09855"
     CDS             complement(7156..7824)
                     /locus_tag="VHR42_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX3027508.1"
                     /translation="MKVIIIGGGQVGAYIAKMLVGNNCSVKLIENREDVCNRLKQELP
                     EDSVVLGSGTDPNVLEQCGIAGVDVLAAVTGADEVNLVASTISKFEFAVPRVIARVNN
                     PKNSWLFNVGMGVDVGLNMADLMGHLVVEEMDLKNMLTLLKLNRGNYSIVQIKVDGKS
                     AAVKKSLKDLDIPAKSLLIALYRGKEVIIPRGNTSIMGDDNILAFADENSQIKLNALF
                     GNPA"
     gene            complement(7843..8493)
                     /locus_tag="VHR42_09860"
     CDS             complement(7843..8493)
                     /locus_tag="VHR42_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX3027509.1"
                     /translation="MKVIIVGCGKLGSGLARHLVQKGHNVTVIDKDPEAFELLGKDFT
                     GEKVVGLGFDRDVLEKAQIRRADAIVACSKSDETNALIGRLAQNIYKVPRVISRLYDP
                     RKAEIYKTLGIQTISTTTWGITRVAEMLSYNQLDSVLTLGSSNVELVRIETPALLVGK
                     TLNELSVIGEIEVVAISRKNKTFLPTLGTALQKHDVIYLAVMSTSVSRLKSLLGLA"
     gene            complement(8505..>8997)
                     /locus_tag="VHR42_09865"
     CDS             complement(8505..>8997)
                     /locus_tag="VHR42_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3027510.1"
                     /translation="GTEKPETGVMDKPPRSTKDTLLNKPLFIKAFLWYGLLESIICMA
                     AYFFVNRTLVASGLIYRQATTMTLASIVFCQIGMVLNCRTEKQSVFKAGLFSNRRVLF
                     GIVFEVCLLSALIYVPFLQGIFNTAAIGLRDWLFLIILPFPILLLEETRKAITRRIDH
                     KKK"
BASE COUNT         2130 a         2193 c         2452 g         2222 t
ORIGIN      
        1 ttaattggtt agaccgaaaa aagccataat acatgtatta atactgctct tccactaaat
       61 ggtaagcatc tttgcggtta attttccgcc ctgttgcgtt cacgccgtgc ggttgcgaag
      121 caaacgttgc gggctgtcag cccgctttcc tcctcggcct tgcagggcaa aaaatttcct
      181 cgcaaatctt atcttctctt tagtggaaga acagtatgac aggaataaaa ataaaagccg
      241 cttttgcggc ggggaggtaa gttatgaaac tttctcagtt aatgaaggat attgccgata
      301 tagataaaaa agaggaccgt gaaattgcgg gaatcacctg cgactcgagg cgggctgccg
      361 aaggctgggc gttcgtatgc attaaaggaa cggcggtcga cggccatcgc ttcgccgaaa
      421 aggcggaagc agacggcgcc tcggtgatta ttgccgagga cgataccggc tgcaaaaatc
      481 agctgatggt gccaactacc cgcaaagcgt gggcgaaaat gtgcgcaaac tggtttggaa
      541 atccgtccga ccggctgcat ttaatcggcg ttaccggcac gaacgggaaa acctctacga
      601 cctatatgct gaaatcggtg cttgaagagt gcgggtataa agtcggactt gtcggaacca
      661 ttcagaatat gatcggggat agggtccttc cttccggcca tacaactccg gacccgtacg
      721 atctgcagag catgttcgct ttgatggccg cggaagggtg tacgtatgcg gtcatggagg
      781 tatcctctca cgcgctggat caggaccgcg tcggcgggtg catgtttgac gcggcggtat
      841 ttacgaattt gactcaggat cacctggatt atcataaaac gatggaaaac tatctggagg
      901 ctaagaaaaa gctgttccgg atgtgccgga aagcggtcgt caacaaggat gatccctggg
      961 ccggcaaatt gatcgaaggg ctgacgtgtc cgatgatcac ctattcgacc tatcaggata
     1021 ccgccgatta taccgcaaag aatattcgcc ctcgccccga cggggtggac ttcgagcttg
     1081 tcggaaccgg cgcgatcgga agggtgcggc tgccgattcc aggcatgttt tcggtctata
     1141 acgcgatggc ggcggcaacc tgcgcgttgg cgctgggtct gccgttcggg cagcttatcg
     1201 atgcgctgtc ccatgtcaaa ggcgtcaaag gccgagcgga gatcgtgccg accggccgcg
     1261 attttaccgt agtgatcgat tatgcccata cgcccgacgg cattgaaaaa atctgcaaaa
     1321 cgatgaagca atgcagcaaa ggacgtcttg tcaccttgtt cggctgcggc ggggaccgcg
     1381 ataaaacaaa acgtccgcgc atgggagcgg ccgccgcttc gctttcggat tttctcattg
     1441 tgacgtcgga taatccgcgt accgaggagc cgggcgcgat cattcaggat attttaacag
     1501 gaataccgga gaacggtacg ccgtatactg tgatagaaaa tcgggtgcag gcgatacact
     1561 gggccattgc gaatgcacgc cccggcgata cgatactact cgcgggcaaa ggccatgaaa
     1621 cttatcagat actaaaagaa ggccggatcc atatggatga acgcgagatc gtagcggatg
     1681 cgctgcgcga aacagagcaa aatagtaagc tgtaaataga tttgaggttg gatggacgta
     1741 tgaatatcat gatgacaatc gtttcggcgt cggctttagt ggcttttctg ctgacggctg
     1801 tttcgggacg cgcggttatt ccgctgctgc ataagctgaa attcgggcag actatccgcg
     1861 atgtggggcc gtcctggcat aaaaataaac agggcacgcc gacgatgggc ggcgtgatgt
     1921 ttatcggagc gacgatcgtg accttcctgc tggcgattgc cgcgtgctat ttcattgttc
     1981 cggcaaaagt gctgaatgtg acgccgctga tcaagacgag gctgtacggc ggcttgctga
     2041 tggcgactgg ctgcggaatc gtcggatatg cggacgacta tattaaagtg gtgaaaaagc
     2101 gcaacctcgg cctgaccgcc cgtcaaaaaa tgttcgggca gctgctggtc gcgcttgctt
     2161 attcgctgtc gatttattta ataagaggaa cggacgttta tgttcctttt gtgggcaatg
     2221 tcgagtttgg catctggttt atcccgttct gtatgtttgt gatcgtctcg atgacgaatg
     2281 ccgtgaactt aacggacggt attgatgggc tgtgcagttc ggtcagcttt attgtctgcc
     2341 tgtttttcat agtcgccgcc ggtgtgacgg gattctccgg ccaaagcatt ttggcctcta
     2401 ttttggcggg agcactcgcg ggattcctgg tttggaacct gcatcctgcg aaagtgatga
     2461 tgggggatac cggttccctg tttatcgggg gtctgattac cgcgcttgct ttcggcatca
     2521 atcagccgtt cctgctgatc ccggtcggca tcatttatat cgtcgaagtt ctgtccgtga
     2581 tgatgcaggt tacttatttc aagattaccc atggcaaacg cctgtttaaa atgagcccga
     2641 ttcaccacca ctgcgaattg tccggctgga gcgaaaataa aatcgtcacg gtgtttacgg
     2701 cgattacgct cctggggtgc gcggctgcct gggtaatttt aatttacgct taaggacacg
     2761 cagggaataa tccccgtacc cgattcgcat gctcttaaat tcaaaatata tggaatactt
     2821 tgactgtttc tgtgaagtat agtatcatag aaaaaccgga aagacgaggt cgattggatg
     2881 gcagtgtcaa gatcggcggc tgtttcagcc gcaccaccaa aaactaaaaa acggcttcgc
     2941 tttttctcgg ttgcgcaggg ttttgacatg ccgtttttaa tcattttaat gattattctt
     3001 gtggtcggac tgatcatgct gttttccgca ggttacgcct attcgtatta ttggaacaac
     3061 ggcgacagct tttattacat taaacggcag ttgatttttg cggtgctcgg cgtgacggcg
     3121 atgctgctta tttcaacggt cgattatcat gtatggcacc gtttcgcaat tccgctgatg
     3181 attatttcct atgtgctgct tggcgtcgtg ctgttattgc cttcgtctat cggcgtctac
     3241 cggtggatcc gtcttcccgg tctcgggcag ttccagcctt ccgaaattgc gaagttttcg
     3301 ctgattctgt tatttgcgca tctgatctcg ctgaaccata aaaaaatgaa aacctttact
     3361 tcggggtatc tgccgtttat cattatatta ggcgttacct gcgggctggt ttttatcgag
     3421 ccgcatctgt ccggtacgat tctgcttctt gcgattggca ttgtgatgat gtatgtcggc
     3481 gggacgcgtc ttttttatct gctcggtacg ctcggcctcg gcttcggtgc cgttgtatat
     3541 atgatattcg taaaaggata tgagcaggac cgtattgacg tatggctgca tccttttgaa
     3601 gcgtatacga cgaataagaa cgaggcatgg cagacgattc agtcgcttta tgcgatcggt
     3661 tccggcgggg taatggggca gggactcggc aattcccgcc aaaagcatct gtttctgccc
     3721 gaaccgcaca acgactttat tttttcggtt atatgcgaag aactcggctt tatcggagcg
     3781 gtccttgtta ttgtgctgtt ctcgctgctc gtatggcgcg gcatcacgat cggcatgaaa
     3841 tcgccggata aattcggttc gcttctggcg gtcggactca ccgtgcagat tggtcttcag
     3901 gccgtgatga atatggcggt cgttacgaat acgattccga ataccggcat cagtctcccc
     3961 ttcttcagtt acggaggtac gtcgctgatc atgctgcttg cacagatggg cgtggtgctg
     4021 tccatctcac gaaacgtaaa cacaaggaag acgtagcgaa gggagtattg cagtatattg
     4081 caatactccc tttgaaaatt aatagaaggc aaccgttttt ctaaccccgc tatccggttg
     4141 ggaacgcata taaagcgtcc cgccatgcgt tccgataaga tgacgacacc cgtcgctgcc
     4201 ttttttctgt tgacttaacc tgataacctt gttaaattta aatacataca aattgtaata
     4261 gttctggagg actcataatg cgcgttctga tgacaggcgg cgggacggcg ggtcatatca
     4321 accccgcttt ggcaatcgcc gcaaaaatca aacaaatgaa tccggaagcc gaaatccttt
     4381 ttgtcggagc taagggaagg atggaaacaa agcttgttcc ggctgccggt tacaaaatcg
     4441 tcacggtcga tgtgcgcgga ttccagcgcc gtctgtcgct gaaaaatgtc ggacgaaacc
     4501 tgtccgccgc cgtacatgtg gtgacggcgg gtgcggcctg ttcccgtata ttgaaagact
     4561 ttaggccgga tatcgcgatc ggtacgggcg ggtacgtcag cggacccatt ttaagaaaag
     4621 ccgcgaagat gggaataccg gtgcttgtcc atgaatccaa cgcctacccg ggcgttacgg
     4681 tgaaaatgct cgcgaaatat gcggccgcaa cgatgatcgc ggaagaagcc gcgcgcaaat
     4741 acctgccggg cggcgcgaat gtgatcgtta ccggcaatcc gctgcgcaac gattttttgc
     4801 atcttgaccg cgaggccgca aggcgggaat tgaacctcga cgaacgtccg cttgtgctgt
     4861 ctgtcggcgg cagtctgggc gccgcgcaca tcaacagcgc gatggctgag gtgctcgcgc
     4921 gcagcgcaaa agagggaaag ctgcagcata tccacgcgac cgggcacgcc ggctatgaag
     4981 aaatgaaaaa aatgctgaag gaaaaaggga tcggcgagaa tacgcccggc atccgggtcc
     5041 gcgaatatat cgacgacatg ccgcgctgta tggccgcggc ggatctggtg atttcacgct
     5101 gcggagcgat gacgctcagc gaactgccgg cggcgggaaa gccgtcgatt ttgattcctt
     5161 ctccgtatgt cgccgagaac catcaatatc ataatgcgat ggcactcgtg cgcaaaggcg
     5221 ccgctgtctg tattgaagaa aaaaatctga cgggcgaagc tttgtggaag tcgattcata
     5281 acatcacttc atctcccgac atgatgcgca gtatgagcga aaatgcaaga aaaacggcga
     5341 ttcttgacgc aaccgagaga atctataaag tcattgaaga tacgctgaag aaagagaaaa
     5401 aataattgtg caattgaaac aggcaggcct tgtgcctgcc tgtatttttt ataagccctt
     5461 ctatccaggg caatgttgac aagttattca aagaaagccg ttccccgcta tccggtaggg
     5521 agcgcatata aatcgtcccg ctatgcgttc cgatgaggcg gtatggagtg cggagggaga
     5581 tttcccgccg gaaaccggcg ctctaatgca gcatagaata tagagccctg cgataaacag
     5641 catacaatat taacaatggc aaagcccgcg gaacagatta tcatgcccga ttcacccata
     5701 cctccccttt tgcataggat ggttttgcag gggtgtgtta ctgcacagtt tattctgtgg
     5761 tttcacgtcc tcccgaaatg cgaaagggtg cattttatgt cgagactgat tattgaaggc
     5821 ggccataaac taaacggcgc ggttacgatt cacggcgcca aaaacggcgc tctgccgata
     5881 ctggccgcga ctctgctggc taaggggcca agtgaaatac aaaactgtcc cgctctttcc
     5941 gatgtagccg catcggttcg gatacttcgt tatctgggat gcatggtcaa ccggtcggaa
     6001 ggctgcgttg ttgttgactc cgccggttta aatcgctgtg atattcccga ttccctgatg
     6061 cgggaaatgc gctcttcgat tgtttttctc ggagcgattg ccgcccgcac gggggaagcc
     6121 cacatgtcgt tcccaggcgg ctgtgagctc gggacccggc cgattgacct gcatctgtct
     6181 gcgctgaggc agcttggaat ggaaatcgtt gaggaaagag gacgactgga atgccgcgta
     6241 aacggccgca tgaaaggcgc cacgatcgca ctggcttttc cgagcgtcgg cgcaaccgaa
     6301 aacattttgc tggcttcggc tacggcaagg gggacgacta cgatcctcaa cgcggcaaga
     6361 gagccggaaa ttgaagacct ctgcgattat ttagttcagt gcggcgcgca tatttcgggc
     6421 gcgggcgaca gcgcgatcat tgtggagggc gtcgatgctc tgcacggatg cgttcatcgg
     6481 gtaatacccg accgcatcga aacggtgacg tatatggctg ccgccgcgat cacgggcggc
     6541 tcgattgtac ttcagaatat ccatcccgag catattgcaa ccgttctccc tgcttttgag
     6601 gaagccgggt gcgaggttac catcggaaac ggcgaattgt atctgtgcgc tcccgaaaga
     6661 ctgcgccgta tccgctcggt tcgcaccatg ccctacccgg gcttcccgac cgatgcgcag
     6721 gctccggcaa tggcgatgac aagcgtcgcc gacggcacga gcgtctttat tgaaaatata
     6781 ttcgaaagcc gttataaaca tgcgggggaa ctcatgaggc tcggggcaaa aattaaaatt
     6841 gaagggcgtg tcgcggtcgt cgagggaatt ccgagattat ccggggctca ggtggaatgc
     6901 accgatttaa gaggaggcgc ggcgctcgta atcgccgggc ttaacgccga gggaatcacc
     6961 gacgtcaccc agcttcacca cattgaccgc ggatacgaaa agcttgaaga aagtttaaga
     7021 agcgtgggcg cggtcgtccg aagaatcgac gaataaatca ctctgtttat agaacatatc
     7081 aaaaaactta aactaaaata acggaatact ccgatggggg agtattccgt tattttagct
     7141 aatgtacctt gcttattatg ccggattgcc gaaaagggca ttcagtttga tttgagagtt
     7201 ctcgtccgca aacgcgagga tgttgtcatc ccccatgatg gatgtattgc cgcgggggat
     7261 aatgacttct ttgccgcggt aaagggcaat cagcagggat tttgccggga tatccaaatc
     7321 ttttaaagat tttttcacgg cagcggattt gccgtccact ttgatttgaa cgatcgaata
     7381 gttgccgcgg tttaatttca gcagcgtcag catattcttg agatccattt cttcgacgac
     7441 aagatgtccc attaaatcgg ccatatttaa tcccacgtcg acgcccatgc cgacattaaa
     7501 cagccaggaa tttttggggt tattgacgcg cgcaatcacg cggggaactg caaattcaaa
     7561 ttttgagatg gtcgaggcca ccaggttgac ttcatcggcg ccggtgacag ctgcaagaac
     7621 atcgacaccg gcgatgccgc actgctccag aacgttcgga tcggtcccgc tgcccaggac
     7681 gacggaatcc tccggcagtt cctgcttgag cctgttgcac acgtcttccc ggttttcaat
     7741 caatttcacg gagcagttat tgcccacaag catttttgca atataggcac cgacctggcc
     7801 tccgccaata ataattactt tcatcgtatg atcctcctcc ccttaagcta atcccagaag
     7861 ggattttaac cgactgaccg aggtcgacat gaccgccaga taaatgacgt cgtgtttctg
     7921 cagggcggtt ccgagcgtcg gcagaaaagt tttgttttta cggctgatcg ccacgacctc
     7981 aatttcgccg atcacggata attcgtttaa tgttttcccg accaggagag cgggggtttc
     8041 gattcgcact aactcgacat tgctgctgcc gagtgtcaat acgctatcta gctgattgta
     8101 actgagcatt tcggcgaccc gcgtaattcc ccaggttgtg gtggagattg tctggattcc
     8161 gagcgtttta taaatttccg cttttctcgg atcataaagt ctcgaaataa cacggggaac
     8221 tttataaata ttctgggcaa gccggccgat caaagcgttc gtttcgtcgc ttttactgca
     8281 ggcgacaatc gcatcggctc tgcgtatctg agctttctcc aacacgtccc ggtcaaagcc
     8341 caaaccgact accttttccc cggtaaagtc tttgcccagg agttcgaacg cctccgggtc
     8401 tttatcgatg acggttacgt tgtgtccctt ttgaacaagg tgccgagcca acccggagcc
     8461 cagcttgcca caaccgacaa taataacttt catacaatgg cctcctattt cttcttatga
     8521 tcaatccgac gggttattgc ttttcttgtt tcttcaagga gtaaaatcgg gaacggcagg
     8581 ataatgagga acagccagtc gcgaagtccg atcgcggcgg tattgaagat tccctgcagg
     8641 aacggcacat aaatcagagc gctcaaaaga cagacttcaa acacaatgcc gaacaggact
     8701 ctgcggttgc tgaacagacc ggctttaaag accgactgct tttcggtgcg gcagttgagg
     8761 accataccga tctggcagaa aacaatcgag gcgagtgtca tcgtcgttgc ctgtctgtag
     8821 atgaggccgg aagctacaag agttctgttt acaaagaagt aagcggccat gcagatgatc
     8881 gattccaaaa gtccgtacca cagaaatgct ttgatgaaaa gcggcttatt gagaagcgtg
     8941 tcttttgtgg aacgcggcgg cttatccatc acgcctgttt cgggcttctc ggtgccg
//
LOCUS       DATALY010000360         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_509030,
            whole genome shotgun sequence.
ACCESSION   DATALY010000360 DATALY010000000
VERSION     DATALY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509030"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHR42_09870"
     CDS             <1..235
                     /locus_tag="VHR42_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027511.1"
                     /translation="DIDLALMVKSKSGEIKTFIKEFDEELRREYFSLLSMPDDSSDYL
                     IDAHVLNEEAENDPSRAYLLHIIKNDSVAIPAG"
     gene            complement(250..582)
                     /locus_tag="VHR42_09875"
     CDS             complement(250..582)
                     /locus_tag="VHR42_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027512.1"
                     /translation="MNDKAAMLVKAVEELEGLEKTSCNVALVFAEMFKDEVNANDEIK
                     LYNGMKRVLKKYSADKGAISALDEFTGVLTGGASLREILTISREEVLSPTAATDISVD
                     NGCRHGGN"
     gene            complement(668..>1514)
                     /locus_tag="VHR42_09880"
     CDS             complement(668..>1514)
                     /locus_tag="VHR42_09880"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027513.1"
                     /translation="IYARKDYEKNREFARRFIDFASDYGLSISLVLREDIIFGIRDGS
                     PFIDLNTCKGMLPRFAINRCMDPLLGKQLEIMGIRVFNPSAVSEVCNDKARTYQRIAA
                     LEIPMVDTVFSTREYLLSRDNLFEYPAVIKSAGGRGGAEVYKAGSLKELREFVRSTNS
                     DRFVVQKLCGMPGRDVRVFVVGRQIVGAVERYSDRDFRANYSLGGAARLYSLSNEQTA
                     LVRRIIDSFKFDMVGIDFIFDSQGNFLFNEIEDVVGTRTLYMNSNIDIVRIYLEHIRN
                     QKQNQ"
BASE COUNT          425 a          379 c          280 g          430 t
ORIGIN      
        1 ggatatagat ttggccttga tggtaaaatc aaaaagcgga gaaataaaga catttataaa
       61 ggaattcgat gaagagctcc gcagggaata tttcagcctt ttatccatgc cggacgacag
      121 cagcgattat ctcatcgatg cgcatgtatt aaatgaagaa gccgaaaatg acccttcaag
      181 agcatatctt cttcatataa taaaaaatga ttccgtagcg attcctgccg gctgaccgga
      241 tggaaaattc taatttccgc cgtgccggca gccgttatct acggatatat ccgttgcagc
      301 cgtgggactg agtacctctt cccttgatat cgtcagaatt tccctcaatg aagccccgcc
      361 ggtcaaaacg cccgtaaatt catccagcgc ggaaatggct cccttgtcag cggagtattt
      421 tttgagaacc cttttcatgc cgttatacag cttgatctca tcatttgcat ttacctcgtc
      481 cttaaacatt tcggcaaaga ccaaagccac attgcacgat gtcttttcaa gtccctcaag
      541 ttcttcaaca gcttttacaa gcatagctgc cttatcattc atcttaccgc ctccgtcagg
      601 caaaaatatt aaaggccgtg tgaccaatta ccggcaacta tagtattaca tcaaaaaaag
      661 attattatta ttgattctgc ttttgattcc ggatatgttc aagataaatt ctgactatgt
      721 cgatattact gttcatgtaa agagtgcgtg tacctaccac atcctctatt tcgttgaaaa
      781 gaaaatttcc ctggctgtcg aagataaaat cgattcccac catatcaaat ttaaaagaat
      841 cgattatacg ccggacaagc gccgtttgct cattgctcag gctgtatagt ctcgccgctc
      901 ctccaagtga ataattagct ctgaaatccc tgtccgagta tctttctact gcgccgacta
      961 tctgcctgcc tacgacaaaa acccgtacat cccttcccgg cataccgcac agcttctgta
     1021 caacaaatct gtccgaattc gtagacctga caaattcccg cagttcttta agcgagccgg
     1081 ctttatatac ttccgctccg cccctgcctc ctgccgattt tattactgca ggatattcga
     1141 acaggttatc ccgggacagc aggtattccc gcgttgaaaa tacagtatcc accatcggaa
     1201 tttccaaagc tgctatccgc tgataagtcc ttgctttgtc attacatact tcagaaaccg
     1261 cagacgggtt aaaaactctt attcccatta tttccagctg ttttcccaaa agggggtcca
     1321 tgcacctgtt aatggcaaaa cgcgggagca tgcctttgca agtattcaaa tctataaaag
     1381 gacttccgtc cctgatgccg aaaattatat cttctcttaa aaccagggat atactgagac
     1441 cgtaatcgga agcaaagtca ataaatcgtc gggcaaattc cctgtttttt tcataatcct
     1501 tacgtgcgta aatg
//
LOCUS       DATALY010000361         7016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_163575,
            whole genome shotgun sequence.
ACCESSION   DATALY010000361 DATALY010000000
VERSION     DATALY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163575"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VHR42_09885"
     CDS             <1..216
                     /locus_tag="VHR42_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027514.1"
                     /translation="GNMYDKYVVMLQILNYKALKDTQIAGNIQIQLMATDPDIRARDR
                     ILKRSEKEGLYNALDIGSIWLERQLGE"
     gene            complement(225..359)
                     /locus_tag="VHR42_09890"
     CDS             complement(225..359)
                     /locus_tag="VHR42_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027515.1"
                     /translation="MFELLSVSPATGDHTNLTVYIVIAVLAVAAAVTAAIVTNFKKKP
                     "
     gene            489..1463
                     /locus_tag="VHR42_09895"
     CDS             489..1463
                     /locus_tag="VHR42_09895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3027516.1"
                     /translation="MTKKISRAIAVLCTLAITFTLCSCSDTSWSIKVNNEKVPAGMYI
                     YFLLSVSSSIKNNATSSDPWSQKIENQDAITYAKDQAVKYASEFVIIENLCKKEKITL
                     TSSEKSAADSQGQQTYSGSSSTFTNNGISQASINRITEDYSLANKLYTKYYGDKGTKT
                     ITDEALKKYEADYICVKHIFFSDKDDSSNKLTGAALTAVTKKVNDVLAQVQKDPSKFD
                     ELMKKYTQDSGGLQQYPNGYTFKKDSTQYVKEFVSGANSMKVGEIKLITSSYGYHIMH
                     RIDLDKTTMKTQYGQELFQKLIDNEVSKAKIVRNQTTINAYNPKTLKS"
     gene            complement(1556..2320)
                     /locus_tag="VHR42_09900"
     CDS             complement(1556..2320)
                     /locus_tag="VHR42_09900"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX3027517.1"
                     /translation="MPSAFKAYSEGNADYIKSMAQKQAECGAEYLDINAAMLPDEKEK
                     LLWAAENVLSVCGDAGLVIDSADPAAIRYVMEKIKARAKKIIINSVTLEKNRLEGILP
                     LAAENRTGIVALPIENSMPQSSEERVENARKLIALLTGKGIPVSWIYLDIVVEAVSAN
                     WEAPRQALLAAAQLRKEYPELHLVAGLSNVSFGLPKRVYINSAFLSSMIAMGVDSAIM
                     DITNTEMRMQLGSALMVNGQDEYCAGYLETYRDVFE"
     gene            complement(2376..4142)
                     /locus_tag="VHR42_09905"
     CDS             complement(2376..4142)
                     /locus_tag="VHR42_09905"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX3027518.1"
                     /translation="MNRLPEKDVILFDGAMGTYIAQKYHTNIKQCEFENIANPGHVLT
                     VHREYIEAGADAIKTNTFSANTAAFSRSDTVEKIILSGCEIAEKAVKGSNTSVFASIG
                     PIAGENMDEVSAEYRKIIDLFLYRGVRNFLFETFSEYSILTELAKYVKENEKDSFVIA
                     ECAVAPDKYTQSGLSAQSILDGMGGVPEIDAYGFNCACGPMHMLNLVREVNFRQKPAS
                     VMPNAGYPTVISGRTVFESTPDYFAQQLIAIRDCGVKFLGGCCGTTPLHIKKASDLLR
                     CAGAAVPAREKEKSETIPAAPGRPHAKLLSEKVVAVELDSPLDADTGFFLSSAIKLSR
                     AGADLITIADCPIGRARADSSMLAAKLRREYDIEAMPHLTCRDRNLNATKALLLGLNA
                     ENVSNVLCVTGDPIPSSDRNEIKSVFNFNSVRLASFIRDLNKTVFTNRPFTVAAALNV
                     NAVTFDAELEKAKRKQDAGVAVFLTQPIYTGAAIENLQKAKRSLNANILGGIMPIVSY
                     KNACFINNEVAGITIPESLIKKYEGADAEQASAIAVETSLATAREISGIADGFYMITP
                     LKKVEIICEIVTQIKGTGKTSG"
     gene            complement(4314..6386)
                     /gene="fusA"
                     /locus_tag="VHR42_09910"
     CDS             complement(4314..6386)
                     /gene="fusA"
                     /locus_tag="VHR42_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX3027519.1"
                     /translation="MRQFKTGEIRNVVLLGHSGKGKTALAESMLFLAGATDRLGRVED
                     GNTVCDFDPEEVKRRVSVSAKIAPLEWKGCKINIIDTPGLFDFEGEALEGLRAGDCAL
                     ITISAKSGVGVGAEKAWKRAGDSNLPRAFYISKIDEERADFYKVFEDLKNHFGTSVCP
                     VIVPFGDNGNKVYVNLIEQSAFTYDGKGKASPCPMPEDDTLGELITALNEAVAETDEE
                     LLDKYYSGEAFTKEELGRGVHKGLTEGVISPVFCGSSVRLAAIDLLLDSIAGIFPSGS
                     DNHHETFTGADGKEVKAAVSETEPLAAVVFKTLADPYVGKLSFFKVISGRLNAESTVV
                     NARTGQPEKVGKLYMLRGKKQTEVEAVAAGDIGAVSKMLSPLTGDTLCSPQRVVNLKG
                     ITFPEPCLSMAVAPKTKGDEDKISQGLHRLMEEDQTFRFINNTETKQQVISGLGEQHL
                     DVIVSKLKTKFGVSVNLEKPKVAYRETIRKKVKVQGRHKKQTGGHGQFGDVWIEFEPC
                     EREELVFEERVFGGAVPRNFFPAVEKGLRECVVHGVLAGYPVVNLKATLVDGSYHPVD
                     SSEMSFKVAATLAYRAGLAQANPVILEPIGLLTVTVPESNMGDVIGEVNKVRGRVLGM
                     NGNEEGMEVIEAEAPMAEMHDFSTVLRSITQGRGYFTFKFARYEEAPPQVAQNVIANS
                     KHVMEAIE"
     gene            6815..>7016
                     /locus_tag="VHR42_09915"
     CDS             6815..>7016
                     /locus_tag="VHR42_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX3027520.1"
                     /translation="MGDISRKTIKEGIGFTSIRDSRFKQSFISVNFLLPLKKETASIY
                     ALLPMVLRRGCEDYPDMTSLNAR"
BASE COUNT         1728 a         1834 c         1627 g         1827 t
ORIGIN      
        1 ggtaacatgt atgataaata tgtggtaatg ctgcagatcc tgaactataa agcacttaag
       61 gacacgcaga tagcaggaaa tatacagatc cagctcatgg cgacggaccc ggatatccgc
      121 gcgcgtgaca ggatcctgaa aagatccgaa aaagaagggc tttacaatgc ccttgacatc
      181 ggaagcatat ggctggagcg tcagcttggg gaataatccg aagctcaggg ctttttctta
      241 aaattggtta cgatagcagc cgttacggca gccgcgacgg caagaacagc aattacgata
      301 taaaccgtaa ggtttgtatg gtctccggtt gcggggctga ctgaaagaag ctcaaacatt
      361 tcgatcttcc tttcatttac tttacgaata ctttatatgc aatataccat tttaagaatt
      421 tccagtcaat acaaaaacgt taaagtttta taggaactat aaaaaattta taaggaccgg
      481 tggatataat gactaaaaaa atatcaagag ccattgctgt gttatgcact ctggcaatta
      541 cgtttaccct gtgcagttgt tcggacacat cctggtcaat caaggtaaac aacgaaaagg
      601 ttcctgcggg aatgtatatt tatttcctgc tcagcgtaag ttccagcatt aagaataacg
      661 cgacaagctc cgacccgtgg tcgcagaaaa tcgagaatca ggacgcgata acctacgcga
      721 aagatcaggc tgtcaaatat gcctccgaat ttgtcataat cgaaaatctc tgtaaaaaag
      781 aaaaaatcac ccttacatcc agtgaaaaaa gcgcagcgga cagccaggga cagcagacct
      841 attccggaag ctcttcgact ttcacaaaca acggcatcag ccaggcttcg atcaaccgca
      901 tcacggaaga ctattctctt gccaacaagc tttacacgaa atattacggc gacaaaggaa
      961 caaaaaccat caccgatgaa gccctcaaaa aatatgaagc tgattatatc tgcgtcaagc
     1021 acatcttttt cagcgacaag gacgacagca gcaacaaact gacaggcgcg gctctgacgg
     1081 ccgtaaccaa aaaggtaaac gacgttcttg cacaggtgca gaaagatcct tccaagtttg
     1141 acgagcttat gaaaaaatac acccaagaca gcggcgggct ccagcagtat ccgaacggct
     1201 acactttcaa gaaggattcc acacagtatg tcaaggaatt cgtcagcggc gcgaacagta
     1261 tgaaagtcgg ggaaatcaag ctgataacct cctcctatgg ttatcatatc atgcaccgga
     1321 tcgacctcga taaaactaca atgaaaaccc agtatggtca ggagcttttc caaaagctca
     1381 ttgacaacga ggtctcaaaa gcaaaaattg tccgtaacca aacgaccatc aacgcttaca
     1441 atccgaaaac cttaaaatca taaacagaat ataaagccgg aaaaaagcgc cgcggaaact
     1501 tccgcggcgc tgatttttgc tcttttcagg catcattccg tacaatggaa tattattatt
     1561 caaaaacgtc ccgataggtt tcaagataac cggcacaata ttcgtcctgc ccgttgacca
     1621 taagcgcgga accaagctgc atgcgcattt ccgtgtttgt aatatccata atggcgctgt
     1681 ccacacccat tgcgatcatt gacgacaaaa aggcgctgtt gatgtatacc cttttcggca
     1741 ggccgaagga aacattcgac agcccggcga caagatgaag ctcggggtat tctttccgaa
     1801 gctgcgccgc tgcaagcagt gcctgtctcg gtgcctccca gtttgccgaa accgcttcga
     1861 caacgatatc cagataaatc cagctgacag ggattccttt acccgtcagc agcgcgatga
     1921 gcttgcgggc gttttcaacg cgttcttccg acgactgcgg catgctgttt tcgatcggca
     1981 gagccacaat gccggttctg ttttccgccg ccagcggcag aatgccttcc agcctgtttt
     2041 tttcaagggt tacggaattg atgatgattt tttttgccct tgctttaatc ttttccatca
     2101 cataacggat cgccgcaggg tcggccgagt cgattacaag cccggcgtcc ccgcaaacag
     2161 acaggacatt ttcagcggcc caaagaagct tttccttttc gtcgggcagc atggcggcgt
     2221 ttatatcaag atattccgcc ccgcattccg cctgcttttg cgccatggac tttatataat
     2281 ccgcgtttcc ttcggaatag gccttgaagg ccgacggaat ggaactgtta agttttttcc
     2341 ccgattatga tcattctctg tcatcctttc gtcttttagc cggaagtttt gcccgtaccc
     2401 tttatctgtg taacgatctc acagatgatc tcgactttct tcagcggggt aatcatatag
     2461 aatccgtccg cgatgccgga aatctcccgg gctgtggcaa gcgacgtttc cacggcaatc
     2521 gcagatgcct gttctgcgtc cgctccctca tattttttta taaggctttc cggtattgtg
     2581 attccggcca cctcgttatt gataaagcag gcgtttttat agcttacaat cggcataatt
     2641 ccgccaagaa tgttcgcatt gaggctccgc ttcgctttct gcaggttttc aatggccgcc
     2701 ccggtataaa tcggctgtgt aaggaaaacg gctacaccgg cgtcctgttt gcgttttgcc
     2761 ttttccagct cggcgtcaaa ggtcaccgcg ttcacattca gcgcagcggc gacggtaaac
     2821 ggccggtttg taaagaccgt tttgtttaag tcccggataa aagacgcaag tcttacggaa
     2881 ttgaaattga aaacgctttt gatttcattg cggtcgctcg acggtatcgg gtcgccggtc
     2941 acgcaaagca cgtttgagac attttcggca ttgagcccca gcagcagcgc ttttgtggcg
     3001 ttgaggttgc ggtcgcggca ggtaaggtgc ggcatggctt ctatatcata ttcgcgccgg
     3061 agctttgccg cgagcatgct gctgtccgct cttgctcttc caatggggca gtcggcaatg
     3121 gtaatcaggt ccgcgcccgc tcttgaaagc ttgatcgccg aagacaggaa aaagccggtg
     3181 tcggcgtcaa gcggcgagtc cagctcgacc gccactactt tttcagaaag cagctttgca
     3241 tgggggcgcc ccggcgccgc cggtatggtt tcgctttttt ctttttcccg cgccggaacg
     3301 gcggcaccgg cgcagcgcaa aaggtcggaa gcttttttta tgtgcagcgg tgtcgttccg
     3361 cagcacccgc caaggaattt caccccgcag tcccggatcg ctatcagctg ctgtgcaaaa
     3421 taatcgggag tcgactcaaa aacggttctt ccgctgataa cggttgggta tcctgcgttc
     3481 ggcatgaccg aagcgggttt ctgccggaaa ttaacctccc ggacaaggtt gagcatatgc
     3541 atcgggccgc aggcacagtt aaagccatac gcatcgattt cggggactcc gcccattccg
     3601 tcgagaatgc tctgcgcgga aagcccgctc tgcgtgtatt tgtccggcgc gacggcacat
     3661 tcggctatta caaacgaatc cttttcgttt tccttcacat attttgcaag ctcggtcagt
     3721 atggaatatt cactgaaggt ttcaaacagg aaattccgga cgccgcgata aagaaaaagg
     3781 tcgatgattt ttcggtattc cgcgcttacc tcgtccatgt tttcgcccgc gatcggcccg
     3841 atgcttgcaa aaacagacgt attgctgcct ttcaccgctt tctccgcgat ttcacacccc
     3901 gacaggatga ttttttcgac cgtgtcggaa cgggaaaaag ctgcggtatt tgcggaaaaa
     3961 gtattggttt ttatcgcatc ggctccggct tcgatatact cacggtgaac cgtaagcaca
     4021 tggcccggat tcgcaatatt ttcaaattcg cactgtttga tgttcgtatg gtatttctgt
     4081 gcgatatagg ttcccatcgc accgtcaaag agaatgacat ccttttccgg cagtctgttc
     4141 acatttccct ccgtaagaaa ttccgcccct gatttggcag gaacaaagtt tttatgcgga
     4201 tattatacca gattttacgg caacagaaaa gagggctgtc tcaaaaaccc gtccggcggc
     4261 atttcgcccg atgtccggtt tccctgagac agccctcaaa gctcatttta tatttactca
     4321 attgcttcca tgacatgttt tgagtttgca attacatttt gggcaacctg gggcggagcc
     4381 tcctcatatc gcgcaaactt aaaggtaaaa taacccctgc cctgggtaat cgaccttaac
     4441 accgttgaaa aatcgtgcat ttccgccatc ggggcttcgg cttcgattac ctccatgcct
     4501 tcctcgttgc cgttcatgcc cagcacccgg ccgcgtactt tgtttacttc gccgatcaca
     4561 tcccccatgt ttgattccgg tacggtaacc gtcaaaagtc cgatcggctc aagaatgacc
     4621 gggtttgcct gcgcaagccc cgcccggtag gcaagggtgg ccgctacctt gaacgacatt
     4681 tcggaagagt ccaccggatg gtaagaaccg tcgacaagcg tcgctttgag attcaccaca
     4741 gggtaacccg ccagcactcc gtgaacgacg cattccctga gccccttttc aactgccggg
     4801 aaaaagtttc tcggcacggc gcctccgaat accctttctt cgaaaacgag ttcctccctc
     4861 tcacagggtt cgaattcgat ccagacatcc ccgaactgtc cgtgtccgcc ggtttgtttt
     4921 ttatgccgtc cctggacttt tacttttttg cggatggttt cacgataggc aacctttggc
     4981 ttttccaggt tgacgcttac gccgaatttt gtcttgagct ttgaaacgat cacgtcaaga
     5041 tgctgttctc caagcccgga aattacctgc tgcttggttt ccgtgttgtt aataaacctg
     5101 aaagtctggt cttcttccat aagacggtgc aggccctgtg aaatcttgtc ctcgtcgccc
     5161 tttgttttcg gcgctaccgc cattgaaagg cagggctccg ggaaagtaat ccctttcaga
     5221 ttcacaacgc gctgggggct gcagagggtg tctcccgtga ggggcgacag cattttagaa
     5281 accgcgccga tatcccccgc ggcaaccgcc tcgacttcgg tttgcttttt gcctcgcagc
     5341 atataaagct tgccaacctt ttccggctgt ccggtccttg cgtttacaac ggtgctttcc
     5401 gcgttgagcc gtccggaaat gaccttaaag aaagatagtt tccccacata tgggtctgcc
     5461 agtgttttga aaaccaccgc cgcaagcggc tccgtttcgc tgactgctgc tttcacttct
     5521 tttccgtccg caccggtaaa ggtttcgtga tggttgtcgc tgccggacgg gaaaatcccg
     5581 gcaatgctgt caagtaaaag gtcgatggcg gcaagccgta cagacgagcc gcagaaaacc
     5641 ggggagatca caccctccgt caggcccttg tggacacccc tgcccagctc ctcttttgtg
     5701 aacgcttccc ctgaataata tttgtcaagc agctcctcgt ccgtttccgc aacggcctcg
     5761 ttaagcgctg ttatcagctc gccgagcgtg tcgtcttccg gcatggggca gggtgaagcc
     5821 tttcccttgc cgtcatatgt gaacgccgac tgttcaatca ggttgacata aaccttattg
     5881 ccgttgtcgc caaaaggaac gatcaccgga cagactgagg tgccgaaatg atttttcagg
     5941 tcttcgaaaa ccttataaaa atccgcccgt tcctcatcga ttttgcttat ataaaaagct
     6001 cgcggcagat ttgaatcgcc cgcgcgcttc catgcctttt cagcgccgac gcccaccccg
     6061 gatttagcgg atatcgtgat aagggcgcag tctcccgcac gcagcccttc cagagcctcc
     6121 ccttcgaaat caaaaagtcc cggggtgtcg atgatattta ttttgcagcc tttccattca
     6181 agcggcgcga ttttcgcaga aacagaaacc ctgcgtttta cttcttccgg gtcgaaatcg
     6241 caaaccgtgt tgccgtcttc aaccctgccc agcctgtcgg tcgcgcccgc aaggaaaagc
     6301 atggattcgg caagtgctgt ttttcctttg ccgctgtgtc cgagcagcac gacattacgg
     6361 atttcgccag ttttaaattg cctcatgatt cgactcatcc tttccggttg caaaatagaa
     6421 tattcggtat gaccgtcaga accgtgcggt atgccgggag ccgcttcgcg gtggtgtatc
     6481 gcatcctttc aaaaaatgat tcggctttga cggaacatta tcaaaaacac atgatgtaat
     6541 atttactatg tttatccgtt accgaaatat tacatattaa tagtatatta ttaatattat
     6601 cctttttcaa gcagaaaatt ttgcattatt gataattact gctgatttct gatgataact
     6661 tttatccata attacaaata tcagtccggc tattcggatt caaaaagtaa atatagccaa
     6721 ctttttcccg ctgtggtata ataagaaagt cgtactctta ataaaattat cgtaaataat
     6781 aattttgact gatattgata aagggggcca aactttgggt gatatttcac gcaaaacaat
     6841 aaaagaaggc atcggcttca cttccatccg tgattcacgt tttaagcaga gttttatttc
     6901 cgtgaacttt ttgctgccgc taaaaaagga aacggcttcg atttacgcgc tgcttccgat
     6961 ggttctccgg cgcggctgtg aggactaccc cgacatgacg tccctgaacg caaggc
//
LOCUS       DATALY010000362         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_763241,
            whole genome shotgun sequence.
ACCESSION   DATALY010000362 DATALY010000000
VERSION     DATALY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763241"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..2183
                     /locus_tag="VHR42_09920"
     CDS             222..2183
                     /locus_tag="VHR42_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEX3027521.1"
                     /translation="MSDRLAVVKIKVLNIIGRMVELEKVANILGKSNVFHPDYALSFY
                     TNTAGFSPVSEENPYAEPLRRLTDTSKSMGKKAEPNGKHTERLPDSVSDWNTYTQKIV
                     QAWNGFSEKRVEHQQKIASLNREIEKVQHFTGLDIRLNEVNECRFIKIRFGFLPAEGY
                     EKLDEIKENPYVVFFPSTSDTSGHWGMYCAPIDQIEEIDRIFSGLNFERTRFTELSGT
                     PEQYLSELDSKRNEERQQIEEINRQINDFWEQEKKDFEILYSWLSEKYICFGIQRYAA
                     RYGDSFILTGWIPAERESGIKKQLDSLSTIRYAADAAEDPEVLTHSPPVLLKNHKIFS
                     PFEFFIKIYGMPRYNEMDPTPVIAFTYALIFGIMFADLGQGICISVIGWLMWKKFHMA
                     LGKAMIPCGIMSALFGTVFGSVFGFEHALDPFYQTVFHLPGKPISVMEGNTTTLVIYS
                     AVGLGIFLVILAIALNIISSLRRGLLENGLFGPNGVAGFVFYVSLIAGALGQIVFGVK
                     LMTPVYVLVLIVLPVIVMLFREMLGGLIEGRPDWKPESMGNYIMQNFFEVFEFLLSYL
                     TNTISFIRVGAFVLVHAGMMLVVFTLAESSSGIGYVLILLIGNAFVIALEGLLSGIQT
                     LRLEFYEMFSRFFDGAGRAFAPVVVSQES"
     gene            2221..2679
                     /locus_tag="VHR42_09925"
     CDS             2221..2679
                     /locus_tag="VHR42_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HEX3027522.1"
                     /translation="MNIVFMCLPVAALVITVAFAIHAVKKGAKKRTALLAQIIAFAAV
                     CLISFSIPVGVSAASTGAAAGAAAGTTTSQAAAAASSDKGLSMLAIALASGIAAIGGG
                     IAVAAAAPAAIGATGENSEVFGKALIFVALGEGIAIYGLLIAILMLNKVQ"
     gene            2705..>2793
                     /locus_tag="VHR42_09930"
     CDS             2705..>2793
                     /locus_tag="VHR42_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit F"
                     /protein_id="HEX3027523.1"
                     /translation="MRFYLISDNVDTQVGLRLAGIEGVVVHKA"
BASE COUNT          650 a          650 c          714 g          779 t
ORIGIN      
        1 gtaaagcgtt ctcccactgt gatttcatat cggcataggg aagcgaaatc ttcctgaagc
       61 ttttgcctta atttcgcttc aacctgatcg cggtgaattt ctgtttcctc aatccccgcc
      121 ataacttttg aataatcggt acggtttttc agataggaag caacttcgct taccgtctga
      181 aataaagaaa ctgcttacga tcgtaaatta catggaaggg agtgtctgat cgcttggccg
      241 ttgttaaaat taaggttctg aatatcatcg gccggatggt tgaattggaa aaagtcgcga
      301 atattcttgg caaatccaat gtttttcatc cggattatgc actttccttt tataccaata
      361 cggctggctt ttctccggtt tcagaagaaa atccgtatgc ggagccgctc cgtcggctga
      421 cggacaccag caaatcgatg ggcaaaaagg cagagccgaa cggaaaacac accgagcggc
      481 tccccgattc cgtcagcgac tggaacacct acacgcaaaa aatcgttcag gcgtggaacg
      541 ggttttcgga aaagcgggtg gaacatcagc agaaaatagc ctctctgaac cgggaaatcg
      601 aaaaggtgca gcattttacg gggcttgata ttcggctcaa tgaggttaac gagtgccgtt
      661 ttattaaaat tcggttcgga ttcctccctg ccgagggtta tgaaaagctg gatgaaatca
      721 aggaaaaccc gtatgtcgtc tttttcccga gtaccagcga cacgtccgga cactggggta
      781 tgtactgtgc gccgatcgac cagatcgaag agatcgaccg cattttttcc ggtctgaact
      841 ttgagcgtac gcgttttacc gaactgagcg gaacgcccga acagtatctt tccgagctgg
      901 actccaagcg gaatgaagag cgtcagcaga tcgaggaaat taaccggcag atcaatgatt
      961 tctgggagca ggaaaaaaag gatttcgaaa tcctctactc atggctttcc gaaaaataca
     1021 tttgcttcgg cattcagagg tatgcagccc gttacggtga cagtttcatc ctcaccggat
     1081 ggataccggc ggaacgtgag agcggcataa aaaagcagct cgacagtctc agtacgatcc
     1141 ggtatgcggc cgacgctgcg gaagacccgg aagtgctgac acactctccc ccggttctac
     1201 taaaaaatca taagattttc agtccgttcg aatttttcat aaaaatttac ggaatgccgc
     1261 gctacaatga gatggacccg accccagtga tcgcgtttac ctacgcctta atcttcggca
     1321 ttatgtttgc ggaccttggt cagggaattt gtatttccgt aatcggttgg ctgatgtgga
     1381 agaaattcca tatggctttg ggaaaagcca tgattccgtg cggaatcatg tcggcattgt
     1441 tcggcaccgt gtttggctcc gtcttcgggt ttgagcatgc tcttgaccca ttctatcaga
     1501 ccgtgtttca cctccccgga aaaccgatct ccgttatgga aggaaacacc actacccttg
     1561 taatctattc cgcagtgggt ctcgggattt tccttgtgat tctggctata gcgctgaaca
     1621 ttatttcctc gttgagacgc ggtttgttgg aaaacggact gtttggtccg aatggagtgg
     1681 cgggctttgt attctatgtt tctctgattg ctggtgcact cggacagatt gtgtttggcg
     1741 taaagctgat gactcccgtc tatgttctgg tgctgattgt tttgcctgtg atcgtcatgc
     1801 tgttccggga aatgcttggc ggactcattg aagggcgtcc tgactggaag ccggaaagca
     1861 tggggaatta tatcatgcag aacttctttg aggtcttcga attcctgctc agctacctta
     1921 cgaataccat cagctttatc cgtgtgggcg catttgtgct tgttcatgcc ggcatgatgt
     1981 tggttgtgtt cacgttggcg gaatcttctt ccggaatcgg ttatgtgctg attctgctga
     2041 tcggaaatgc atttgtaatc gcactggagg gcctgctttc cggaattcag accttaagac
     2101 ttgaatttta tgaaatgttc agcagattct ttgacggggc tggccgagcc tttgcaccgg
     2161 tcgtcgttag tcaggaatct taaaataact taattttgca attatttagg aggaaaaatc
     2221 atgaacatcg tatttatgtg cctgcctgta gctgctctgg tcatcaccgt tgctttcgca
     2281 atccatgccg ttaagaaggg cgcaaaaaag cgtaccgctt tacttgcaca gattattgct
     2341 tttgctgcgg tatgtctgat ttccttcagc attccggtcg gtgtttccgc cgcttccaca
     2401 ggagccgctg caggtgccgc cgcgggtaca acaaccagtc aggctgctgc tgccgcttct
     2461 tctgataaag gtctgagcat gcttgccatt gctcttgctt ccggaattgc cgctattggc
     2521 ggcggtatcg cagttgcggc tgcagctcct gccgcaatcg gcgcaaccgg cgagaattcg
     2581 gaagttttcg gtaaagcact tattttcgta gctcttggcg aaggtattgc catctacggt
     2641 ttgctgatcg ctatcctgat gcttaacaag gtacagtaac ggttcaaaat acggaggttc
     2701 gcgcatgcgt ttctatttga tcagtgacaa tgtcgacact caggtcggtt tgcgcctggc
     2761 gggcattgaa ggcgtcgtgg tccacaaggc cga
//
LOCUS       DATALY010000363         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_78328,
            whole genome shotgun sequence.
ACCESSION   DATALY010000363 DATALY010000000
VERSION     DATALY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78328"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..324)
                     /locus_tag="VHR42_09935"
     tRNA            complement(248..324)
                     /locus_tag="VHR42_09935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(288..290),aa:Arg,seq:ccg)
     gene            complement(444..1766)
                     /locus_tag="VHR42_09940"
     CDS             complement(444..1766)
                     /locus_tag="VHR42_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX3027524.1"
                     /translation="MEDIQEFSMNNEWKNFADTKPVYFDFMEDMIYFFMFFPALVFAD
                     SFSGSDPLIFLKILVVPCSMLLMTVNRRLTRKFHWFALIEILIIFGVFLIPTGQINRI
                     TFTASATGAAFVSFHKVQNILRMKQYRNDPDVAVNFSKFLGYLTLAGGLAAIYFTYML
                     ALSQKKTQTVLCCFVSFVLMLTLYEVYIHIAGTYCLLEWKKSKTGSLIKIKDFNLLFA
                     VWAVILFSLTGLISYVLVYSTGIDRVDSTILSIFRQNNIGVPSSSGGNQIDFTKTLNR
                     MALNNKPGYAAMIFSKVLNLLLIFLAAVIGIALLLLITRALYSILKKFRINLNEESST
                     VFSFEEASAEIRQKIKTGIGKTFRLNGNSNRLSIRRLYYRTIKRHQKSGLEVYPSDTP
                     KEIMLKIKQQSGLDLDLATNLYEKARYGESDSTKQEMEQMKNLLKSRS"
BASE COUNT          520 a          391 c          429 g          521 t
ORIGIN      
        1 atgacttctt ttccatgctt tcaacacgca gctttctgtg aatatattat cccttttctg
       61 cgtgtccggc aagcttggct ggggtcagtg agcattatat cgagaatttt ggtaaaagca
      121 aaaaaagcaa ggcagaatgg gcgcttttgt acccacctgt cttgctttta cttgtgttat
      181 cagcgtttcc ataattgaat aattaaaaga acgaaccaga cattgtataa atgtctggtt
      241 catttcgtgg tgcgcttgac aggagtcgaa cctgcgacct ttagagtcgg agtctaacgc
      301 tctatccagc tgagctacaa gcgcatattg agcaatcgtc catttgaaaa acatgtcttc
      361 aaatgggctt catcagacat gttgctcaac tattatagca gaagcgctaa tctttgtaaa
      421 ggctgctttt tcaatgggta gagtcagctt cgggatttaa gcagattctt catttgttcc
      481 atttcctgct ttgtgctgtc gctttcccca taccgtgcct tttcgtaaag attcgtagca
      541 agatccagat ccagccccga ttgctgtttg attttgagca taatttcctt tggggtatcg
      601 gaaggataaa cctccaaccc acttttctgg tgccttttta tcgttcggta ataaagccgg
      661 cggatggaga ggcgattgga atttccgttc aggcggaatg tttttccgat gcccgttttg
      721 attttttggc gaatttcggc ggaggcctct tcaaaggaaa aaaccgtgct gctctcctcg
      781 ttcagattga tccgaaattt cttcagaata gaatacaggg ctctggtaat cagcaggagt
      841 agggcaatac ctatgacagc cgcaagaaaa atcaataaga ggttgagtac cttggagaag
      901 atcattgcag cgtacccggg cttgttattc aacgccatac ggttcagagt tttggtgaaa
      961 tctatttgat ttccgccgga agaagacggg acgccgatgt tattctggcg aaaaatcgaa
     1021 agaatggtgc tgtcgacgcg gtctattccg gttgaataga caagaacata ggagatcagg
     1081 ccggtgagtg agaataaaat cacagcccaa acggcaaaaa gaagattaaa gtctttgatt
     1141 ttaataagag accctgtttt acttttcttc cattccagca ggcagtaggt tccggcaatg
     1201 tgaatataga cttcatacaa ggtcagcatc agaacgaatg atacgaagca gcagagaacc
     1261 gtttgtgtct ttttttgcga cagcgcaagc atataagtga aataaatcgc ggcaagcccg
     1321 cctgccaggg tcagatatcc cagaaatttg ctgaaattca ccgcaacatc cgggtcgttg
     1381 cggtattgtt tcatccgcaa aatgttttga actttgtgaa atgatacaaa agcagccccg
     1441 gttgcgctgg cagtaaacgt gatccgattg atttgaccgg ttggaatcag gaagacaccg
     1501 aaaataatca gaatttcgat taaggcaaac caatgaaatt ttcgggtcag tcgtctgttg
     1561 acggtcatca gcagcatgga gcacggcacg acgaggattt tcaggaagat cagtggatcg
     1621 gatccggaaa aactgtccgc aaacaccaaa gcggggaaaa acataaagaa ataaatcatg
     1681 tcttccatga aatcaaaata gaccggtttc gtatcggcaa agtttttcca ctcattattc
     1741 atggaaaact cctgaatgtc ctccatcaat aatccgcctc gcacatcgta agattttccg
     1801 tattcggaat attggtttta gagttgctgt cccggatcaa aatccattgc aggctgtgac
     1861 c
//
LOCUS       DATALY010000364         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_683330,
            whole genome shotgun sequence.
ACCESSION   DATALY010000364 DATALY010000000
VERSION     DATALY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683330"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VHR42_09945"
     CDS             complement(<1..1071)
                     /locus_tag="VHR42_09945"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3027525.1"
                     /translation="MLKNLCKEYTDLNQQELDKLARLEEMLPLIADLMNADVFIDCLT
                     RDPDVAVVAAEAKPKSHPSLYQGSVAGQLALRKNEPAALRTLEIGMETRDLKALTQEQ
                     IMVKQSVVPIKFGEKVIGALIMEQDVTNAMNQNARMEILSETAEQLTQTLMNFQEGDG
                     DNPITHHLNDAILVFDQDGLSTYANPVAEELYRKLGYLDRITGMHFDNLSLNETKFRN
                     LLENQNLAVSEINIGGLTLQVKYARMRIGGNQKGIIMLIRDVTEVKEKEKELILKSVA
                     IREIHHRVKNNLQTIASLLRLQSRRLNDQESKIFFQESINRVLSMAATHEILAQNGVD
                     DVDIMTLLLKLKASTFNFESRPD"
     gene            complement(1104..2249)
                     /locus_tag="VHR42_09950"
     CDS             complement(1104..2249)
                     /locus_tag="VHR42_09950"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3027526.1"
                     /translation="MFDSVYQFSMPRETWIGNRSVEKLGPVLKKNNISKCLLVTDSFM
                     AGSETGLKIQQILEQYQIDLSVYSGVTPNPTMDEVTEGLGQFLQQECDALISLGGGSP
                     HDCAKGIKYSVLKGNVCSLEKVFFAAVNTTAGTASEITSFAVITDDEKHSKLTIVDEE
                     LIPDVAIDDPSLMLDMPRSLTAATGMDAMTHAIEAYVAKGRNYLTDSSAVSAIRLIDR
                     YLAKACREGHDLEARNGMVYAQYMAGMAFSNAGLGIIHAMAHQLGGLYNLPHGVCNAA
                     LLPYGVRFNLNAGCDLYGELAKSLQLCTLSIPNKLAGERLVDYLIRFNAKLEMPQDLK
                     QLNVNKEDFEKLAEMAMHDVSLTSNPVGADQEAIRSIFEKAYLGECK"
BASE COUNT          546 a          590 c          553 g          679 t
ORIGIN      
        1 gtctggccgg ctctcaaaat tgaaggtgct tgctttcaat ttcaaaagaa gcgtcataat
       61 atcgacgtcg tcaacaccat tttgggccag aatttcatgg gtcgcggcca tgctgagaac
      121 ccggttgatg ctttcctgaa agaagatttt tgactcctga tcattcagcc gtctgctttg
      181 aagtctcagc aggctggcaa tggtctgcag attgtttttg acccggtgat gaatttcacg
      241 aatcgcaacc gatttgagaa tcagctcttt ttccttttct ttaacttccg taacatcccg
      301 gatgagcata ataatgccct tttggtttcc cccgattctc attcgtgcat atttaacctg
      361 tagcgtcaga ccgccgatgt ttatttccga aaccgccaaa ttttgatttt cgagaaggtt
      421 ccggaatttc gtttcattaa gtgaaagatt gtcaaaatgc ataccggtaa ttcgatcgag
      481 atagccaagc ttgcggtaaa gctcctctgc aaccgggttt gcgtaagttg agaggccgtc
      541 ctgatcaaaa accagtattg cgtcgttcag atgatgggtg atggggttat ccccgtcgcc
      601 ttcctgaaag ttcatgagcg tttgggtcag ctgttcggct gtttccgaaa ggatttccat
      661 ccgggcgttt tgattcatgg cgttggtaac atcctgctcc atgattaacg ctccgatcac
      721 cttttcaccg aatttgatcg gaacaacgct ctgctttacc ataatctgtt cctgtgtaag
      781 cgcttttaaa tccctggtct ccattccgat ctcaagtgtc cgcagagcgg caggctcgtt
      841 ttttcgcagg gccaattgcc cggcaacact gccctgatac aaagaaggat gtgattttgg
      901 tttggcctcc gcggcaacaa ccgccacatc cggatccctt gtaagacagt caataaaaac
      961 atccgcgttc ataagatcgg caatcagagg aagcatttct tccagacgcg ctaacttgtc
     1021 cagttcctgc tgatttaaat cggtgtattc tttgcagagg tttttgagca taagaccctc
     1081 ccgaacaaaa acttgagccc tgtttatttg cattcgccaa gataggcttt ttcaaatata
     1141 cttctgattg cttcctgatc ggcgccgacc ggattgctgg tcaaggacac gtcgtgcatt
     1201 gccatttcag caagtttttc aaagtcttct ttgttcacgt tcagctgttt gaggtcctgc
     1261 ggcatttcca gttttgcatt aaaacgaatc aggtaatcga ccagcctttc ccccgccagc
     1321 ttattcggta tggacagggt gcatagctga agagatttcg ccagttctcc atagagatca
     1381 cagccggcat tcagattgaa gcgtacgcca tagggaagca gcgccgcatt gcagacgccg
     1441 tgaggaaggt tatacaaccc gccaagctga tgcgccatgg catggatgat tccgagcccg
     1501 gcgttggaaa aagccatccc tgccatatat tgggcataaa ccattccgtt gcgggcttcc
     1561 agatcatggc cttcccggca agccttagcc agataccggt caatcagcct gattgcgctt
     1621 acggccgaac tgtcggtcag ataattacgg cctttcgcca cataggcttc aatggcatgg
     1681 gtcatggcat ccattcccgt cgcggcggtc agggatcggg gcatatcaag catcagcgaa
     1741 gggtcgtcaa ttgcgacatc ggggattagc tcctcatcca caatggtcag cttcgagtgt
     1801 ttttcatcgt cggttataac cgcgaaactg gtgatttcac ttgccgtgcc tgccgtggtg
     1861 tttactgcgg cgaagaaaac cttttcaaga ctgcagacgt tcccttttaa aacagagtat
     1921 ttaattcctt tggcacaatc atgggggctg cccccgccaa gagaaatcaa ggcgtcgcat
     1981 tcctgctgca aaaattggcc caggccttcc gtgacttcgt ccatggtcgg gtttggcgtc
     2041 acaccgctgt aaacactgag gtcaatctga tattgttcca gaatctgctg gatttttaat
     2101 ccggtttcgg agcccgccat aaacgagtca gttaccagaa ggcatttcga aatgttgttt
     2161 tttttcaaaa caggccccag tttttcaacc gatcggtttc cgatccaggt ctctctgggc
     2221 atgctgaatt gatagacgga atcaaacatt agattttccc cctgcaattt tgttctgctg
     2281 agaaattttc catgacagac agagccagat cgtagagcgt ggttctttgg ttcatactgt
     2341 catttctgag ttttttataa gcttcgtc
//
LOCUS       DATALY010000365         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_852254,
            whole genome shotgun sequence.
ACCESSION   DATALY010000365 DATALY010000000
VERSION     DATALY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852254"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VHR42_09955"
     CDS             <1..441
                     /locus_tag="VHR42_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX3027527.1"
                     /translation="CVELGVKRIVPVITSRCVSRPDEKAIVSKVERWNKIAVQAAKQS
                     GRGILPAVEQAYGFRQALGKLASHESGILFYEQNGGRLSESLKGTEQDIGIFVGPEGG
                     FEQEETDAAAQCGIAIAGLGPRILRTETAPLCALSAIMFATGNI"
     gene            complement(540..1625)
                     /locus_tag="VHR42_09960"
     CDS             complement(540..1625)
                     /locus_tag="VHR42_09960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3027528.1"
                     /translation="MSKKFRKLPAILCVIFAALSFTGCADTTWAVKYNSITIPSGVYI
                     TYLINAKGDLESQAESSSSSSSSSSSAGLGGTSISGSSSSSTSSATDIWSQKIDGTNA
                     LTWAVSQATLNCKKLAYIEQQCTEKKLTLTSTEKTTANSYADQYFSTYTVLNSNGVSS
                     SSLEKIVQSSYLGDKLFTYYYGTGGSKAIPDSQAKDYYVKNYVHIKQIFFNKYDSSSN
                     LLSDDKLAKLKTKANQVLALAEKDKAGFDALVKKYNEDPGMTETPDGYIFNKSSSYIQ
                     NFKDTAFSMKVGDIKLIETTEGYHIMYKVNVDPNASTYSQNKSGVLHAMKDTEFNTMV
                     ENALKNEKFQVNSQTINRYNPKNLKDS"
     gene            1818..>2426
                     /gene="alr"
                     /locus_tag="VHR42_09965"
     CDS             1818..>2426
                     /gene="alr"
                     /locus_tag="VHR42_09965"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3027529.1"
                     /translation="MSRFLKRTWAEVDLDAIAHNYKAIRSYIREGCKIMAVVKADAYG
                     HGAPLAAQELQTLGADYFGVSNIEEAMQIRRSGITKPILILGFTPVNYARNLIDEQIT
                     QTVFSYEYGRELSNAAREAGGKIRAHIKMDTGMSRIGFVVEDEPAPDVIGRIEKTVAL
                     PGLEFEGVFTHFAVADEPENNFTQTQFERFTRALDALEAKGVT"
BASE COUNT          581 a          520 c          646 g          679 t
ORIGIN      
        1 tgtgtggagc ttggtgtaaa acgtattgta ccggtcatta cgtcaagatg cgtttcccgg
       61 ccggatgaga aggcaattgt gtccaaagtc gagcgttgga ataaaattgc cgtgcaggcc
      121 gcgaagcaat ccggccgcgg catattaccc gccgttgagc aggcgtatgg ttttcgtcag
      181 gccttgggga aactggcttc tcacgaatcg ggaattttgt tctatgagca aaacggcggc
      241 aggctttccg aatcgcttaa aggaacggaa caggatattg gaatttttgt cggaccggaa
      301 ggcggcttcg aacaggagga aacagacgcc gcggcgcaat gcggtattgc gattgccggg
      361 cttggccccc ggatattgag aacggaaacc gcgccccttt gcgctttgtc ggctattatg
      421 ttcgcaacgg gcaatattta atcaggcccc ggtgcttcac tgaaacaaaa tagtaaaagc
      481 aaaagggtct gccgcaaacg gcagaccctt ttgctttttt attatcagaa tgaaaattct
      541 tatgaatcct tcagattttt agggttatag cggttaatgg tctggctgtt aacctgaaat
      601 ttctcatttt tcaacgcgtt ttccaccatt gtattgaatt ccgtatcttt catcgcgtga
      661 agtacgcctg atttattctg actgtaagtt gaggcgttgg gatcgacatt gactttatac
      721 atgatgtgat acccctcggt agtctcaatc agcttgatgt ctccgacttt catcgaaaag
      781 gcagtgtctt taaagttttg aatgtagcta ctgcttttgt tgaagatata tccatccggg
      841 gtttccgtca tgccggggtc ttcgttgtat tttttgacaa gcgcgtcaaa accggctttg
      901 tccttttcag caagcgcgag gacctgattg gcctttgtct tgagcttggc gagtttatca
      961 tccgataaga gattgctgct gctgtcatac ttattaaaga atatttgctt gatgtggacg
     1021 tagttcttca cataataatc tttggcctga ctgtccggaa tcgctttgga gccgcccgtg
     1081 ccgtaataat aggtgaacag tttatcgccc agataagaac tttggacgat tttctccagc
     1141 gaagaggagg aaacgccgtt gctgttgagt acggtgtaag tgctgaaata ctggtcggcg
     1201 tacgagttgg cggttgtttt ttcggtcgac gtcagcgtca gttttttctc tgtgcattgc
     1261 tgctcgatat aagcaagctt tttacaattg agagtggcct ggctgaccgc ccaggtcagg
     1321 gcgttcgtcc catcaatttt ttgggaccag atatcggtag cggatgaggt gcttgatgaa
     1381 ctggaaccgc tgatcgaggt tccgccgagg ccggccgacg aagaggaaga cgacgaactt
     1441 gagctgcttt cggcttggga ctccagatcg cctttcgcgt tgatcaggta ggttatgtat
     1501 acgcctgacg ggatcgtgat actattatat tttaccgccc atgtggtgtc cgcacatccg
     1561 gtaaaggaca gggcggcaaa aatcacgcag agtatcgctg gtaatttcct gaattttttt
     1621 gacattacgg gtcctccctt gaaagactgt ttaaaaaatt atataccaat attttgattc
     1681 tgtccacaat atttacgcga aagcctcaga ggatggagac tgatattttc aggttgattt
     1741 ttgaatgttt aagcttataa tggtatatgt atcgaattta agcaaataaa ctgaagacag
     1801 aatccggagg taacaatatg tcccgctttt taaagagaac atgggcggaa gtcgatcttg
     1861 acgccattgc ccataactat aaagccattc ggtcttatat ccgtgagggg tgcaagatca
     1921 tggccgttgt caaggccgat gcttacggtc acggcgctcc gctggcagcg caggagcttc
     1981 agacgctggg ggctgattat ttcggcgttt ccaatattga ggaggcaatg cagatccgcc
     2041 ggagcggcat cacaaagcct atccttattc ttggctttac ccctgtaaat tatgcgcgga
     2101 acctgattga cgaacagatt acccagactg ttttcagtta tgaatatggc agggaattgt
     2161 cgaacgcggc tagagaggcg ggcgggaaaa tccgggcgca catcaaaatg gataccggaa
     2221 tgagccgcat cggatttgtg gttgaagacg agcctgcgcc ggatgtgatt ggtcggattg
     2281 agaagactgt cgccctgccc ggcctcgaat ttgaaggggt ttttactcat tttgcggttg
     2341 cggatgagcc agaaaacaat tttacccaaa cccagtttga gcggtttacc cgcgcgcttg
     2401 acgcgcttga agcgaaaggg gtcaca
//
LOCUS       DATALY010000366         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_509802,
            whole genome shotgun sequence.
ACCESSION   DATALY010000366 DATALY010000000
VERSION     DATALY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509802"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VHR42_09970"
     CDS             <1..297
                     /locus_tag="VHR42_09970"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX3027530.1"
                     /translation="PDTKGMIAALSADSLISVGEGLRNVLEPVAELEEVARIEKLMNQ
                     KGAVGACMSGSGPTVFGIFDDITVAKACVQALGREYREVYLTEPSCHGCEILEN"
     gene            360..1037
                     /gene="spoIIR"
                     /locus_tag="VHR42_09975"
     CDS             360..1037
                     /gene="spoIIR"
                     /locus_tag="VHR42_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HEX3027531.1"
                     /translation="MNKLEKSILCGFLIAVIVSMASGFTTFAGQCSDIRQNVLRLHIL
                     ANSDSSADQALKIKVRDKILAKSGELFQKSDSKADAIQKADARLEEIRKTAQNEVVAE
                     GYDYKVQAKIVHMYFTTRTYGNITLPAGNYDAVRITIGKAQGHNWWCVLFPPLCLPSA
                     EGTEKLSDVLNSDEVGIVTGSGDKTIVIKFKVLEWYEDAKNFISDGCSKAKDALAGTA
                     DWVSGVR"
     gene            complement(1265..2026)
                     /locus_tag="VHR42_09980"
     CDS             complement(1265..2026)
                     /locus_tag="VHR42_09980"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HEX3027532.1"
                     /translation="MLRLVFGRAGSGKTAFTLEKADEIARMGGEAVVIVPEQFTFETE
                     RVLTGKYGPQTCLNIEVLSFSRLCERVFSLAKETLDPLLGTKPDPELVRQLRDGILHY
                     DYITGSHDLMIHNYGPGRTIASIHAEVPCDVSVLKLHESIDLAEKEMSEKLDIILIIH
                     MDPLNNDDEKVRAAYSETIDLLRNYSEVKSIHDFRIVGDSEKKNLVFDIVVDSKKVVS
                     KKDEQALKEKIDATIKQNHPLYNAIVSIDQDYTEN"
BASE COUNT          578 a          524 c          560 g          579 t
ORIGIN      
        1 cccgacacga aggggatgat cgcggcgcta tcagccgaca gtctcatcag cgtcggcgag
       61 gggcttcgca acgtgcttga gccggtggcg gagcttgaag aggtcgcacg gatcgaaaag
      121 cttatgaacc aaaaaggcgc ggttggcgcc tgcatgagcg gaagcgggcc gacggtcttt
      181 gggatttttg acgacatcac tgtggctaag gcgtgtgtgc aagctctcgg cagggaatac
      241 cgcgaggttt atctgaccga gccttcctgc catggatgtg aaatcctgga aaactgaata
      301 ttaccaaaat tttccgtcca taatttgatc gaacacatga aaggacggaa aattacatta
      361 tgaataaatt ggaaaaatcc atactgtgcg gattcctgat tgccgtaatc gtttccatgg
      421 cgtccggttt taccaccttc gcaggccagt gcagcgatat ccgccagaat gttctcaggc
      481 tgcatatcct ggcgaattcc gacagcagcg cggatcaggc gctgaaaatc aaagtcaggg
      541 ataagatcct tgcaaaatcc ggtgaacttt ttcaaaaatc cgactcgaaa gcggacgcca
      601 tccaaaaagc ggacgcgcgt ctggaagaaa tcagaaagac ggcgcagaat gaagtggtgg
      661 ccgagggtta tgattataaa gttcaggcca aaatcgtcca tatgtatttc actaccagaa
      721 catacggcaa catcaccctg ccggcaggaa attacgacgc ggtgcgtatc acgatcggca
      781 aggcgcaggg ccacaattgg tggtgtgtgc tgttccctcc gctctgtctg ccgtcggccg
      841 agggcacgga gaagctcagc gatgtgctca acagcgatga ggttggcatt gtgaccggtt
      901 ccggtgacaa aacgattgtg ataaaattta aagttcttga gtggtatgaa gatgccaaaa
      961 attttatcag tgacggctgc tccaaggcga aagatgctct ggccggcaca gcagattggg
     1021 tgtccggcgt ccgataagga attgatccgg ccactggtat ccgcctgcga cagagcggta
     1081 tgtactgcaa cctgttttta aaaaattaat cccggtcaat cgcgtttgac acagcagagg
     1141 ccgcctcctc gtgaggcggc ctttacgtta attacggtgg gagcatagtt cgcgtcttta
     1201 gaatgtccgg ctttccgaaa agacgacagc agaaattttc cactgttcgt tttaacgacc
     1261 ggctttaatt ttccgtgtaa tcctggtcga tactgacaat ggcattataa agcggatggt
     1321 tttgtttgat cgtagcgtca attttttctt taagcgcctg ctcgtctttt tttgagacga
     1381 cttttttact gtccacaaca atatcaaaga caagattctt cttttcgctg tcgccgacga
     1441 tccgaaaatc gtgaatggat tttacttcgc tgtaattccg gagcagatca atcgtttccg
     1501 aataagccgc gcggactttc tcgtcgtcat tattcagagg gtccatatga atgattaaaa
     1561 taatatccag tttctcagac atctcttttt cggcaagatc gattgattcg tgaagcttta
     1621 aaacagaaac gtcgcatgga acctcggcgt gaatcgaggc aatggttctg cccggaccgt
     1681 aattatggat catcaggtca tgcgatccgg taatgtagtc ataatggagg atgccgtctc
     1741 tgagctgtct gacaagttcc gggtccggtt tcgttcccag aagcggatca agagtctctt
     1801 tggccagact gaaaacccgc tcgcaaagcc ttgaaaagct gagaacttcg atattcaggc
     1861 aggtctgggg gccgtatttc ccggtaagga cgcgctctgt ctcaaatgtg aactgctcgg
     1921 gaacaatcac cacagcctcc ccgcccattc gggcgatttc atcggctttt tccagcgtga
     1981 atgcggtttt tccgcttccg gcccggccaa ataccagcct taacatgatt ccttccccca
     2041 gtcgtttttt taatctgtaa tgtatgcaaa aagatgcctt tcgttcgcaa atgcatgtga
     2101 attgccaaac gaagacatca taaagtcatt gattcggcag atattaatta taacatgaat
     2161 ccatttccgt tcaaacctta tggcaggaat ctatgcctgt taatatacag gtaaaacaaa
     2221 ttatggtata atgatcttta t
//
LOCUS       DATALY010000367         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_510137,
            whole genome shotgun sequence.
ACCESSION   DATALY010000367 DATALY010000000
VERSION     DATALY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510137"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..832)
                     /locus_tag="VHR42_09985"
     CDS             complement(146..832)
                     /locus_tag="VHR42_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX3027533.1"
                     /translation="MLEQLTKRINIVTGHYGSGKTNLSVNLAKDLNRSGKKVSLVDLD
                     IVNPYFRAADLTKELEAEGIRVIAPIYANSNLDIPALPPAIYSVFNDKTYTAVIDVGG
                     DDAGAAALGRFSDLITEENDFDHFYVINARRILTQTPEETVVILKEIEKSSRIPVTAL
                     VNNTNLSKETDAGVVRASCEYARRVSELSGLKIKFTSVRKDIAAQLGDLPDCGGPVYP
                     VDIDVQLPWD"
     gene            1034..1747
                     /locus_tag="VHR42_09990"
     CDS             1034..1747
                     /locus_tag="VHR42_09990"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX3027534.1"
                     /translation="MKKRPIFQNRSTKTIPKAARAIIAALLILVCASFALFLAIRPHN
                     GGAASQTSLSVKTAEKSVSGSSSSSSQKPVSSSSENKSSSSAVSSSAQTSSVSQQSSS
                     SSSKASAVSIILPLRIRVDTSAQRVFVYDAKNKLVKEFVCSTGTDGNDTPKGNYTVQE
                     RGYSFFSEKYQEGAYYWVQFYGNYLFHSVPFDKGRKIEAVEAAKLGTKASHGCVRLSI
                     ENAKWMYVNIPRGTKVVIE"
BASE COUNT          543 a          536 c          478 g          512 t
ORIGIN      
        1 tgattttctt cgggcatgca tgagtgcaaa gcccgcagcc cttgcaaata tcctcgttta
       61 ctgtaacctt cggcatttta acacctccat taaaaattcc gccgccattt tggccgttca
      121 aagactgcac ggccgcgccg cgatttcagt cccagggaag ctgcacatct atatcgacag
      181 gatagaccgg cccgccgcaa tccggcagat cgcccagctg agcggctata tctttcctga
      241 cgctggtaaa tttaattttc agccccgaca gttccgagac gcgccgggca tattcacagg
      301 aagcccggac caccccggca tccgtctctt tggaaaggtt tgtattatta acaagcgccg
      361 tgacaggaat gcgggaagac ttctcaatct cttttaagat tacgaccgtt tcctccggcg
      421 tctgtgtcag aatgcgcctt gcatttatga cgtagaaatg atcaaaatcg ttttcttcgg
      481 taatcaggtc tgaaaaacgg ccgagcgcag cggctccggc atcgtctccc ccgacatcaa
      541 tcacagctgt ataggtcttg tcgttaaata ccgaatagat tgccggcggc agcgcgggaa
      601 tatcaagatt gctgttggcg taaatcgggg caatcacccg gattccttcc gcctcaagct
      661 cctttgtcag atcggcggcg cggaaatacg ggttgacaat atccagatcc accaggctaa
      721 cctttttgcc ggaccgattc aggtctttgg caagattgac ggaaaggttg gtctttccgc
      781 ttccataatg accggtcaca atattgattc tctttgttaa ttgctctaac attccggcac
      841 ctgtgccttt cgaagcagtg tttgtaagca aacggagttt ttcgatacaa aactattata
      901 gcatctttgt aaagccatta caattgcagg aaataccaaa ctgatcctgc attttgattg
      961 cgcaatggag tcaatgctcc tataataaga gaaaaagaat cgactgcaat ttcaattttc
     1021 ggaggaaatc agaatgaaga aacgacctat tttccaaaac cgttccacaa aaacaatacc
     1081 gaaagcagcc cgagcaatca tcgcagcatt actcattctg gtctgcgcgt cattcgccct
     1141 ttttcttgcg atccgccctc ataacggcgg cgccgcgtcg cagacgtctt tatccgtaaa
     1201 aaccgctgaa aagtcggttt ccggaagttc gtcctcatct tctcaaaaac cggtctcatc
     1261 aagctcggaa aacaaatcgt cctcatcggc tgtttccagc agcgcccaga cgtcttccgt
     1321 ctcccagcag tcgtcttcct catcgtcgaa agcttcggcg gtcagcatca tcctgccgct
     1381 tcggattcgt gtggatacat ctgcccagag agttttcgta tacgatgcca aaaacaaact
     1441 tgtcaaagaa tttgtctgct cgaccggaac agacggcaac gatacaccca aaggaaatta
     1501 tactgttcag gaacggggct actccttctt tagcgaaaaa tatcaggagg gcgcctatta
     1561 ctgggttcag ttctacggaa attatctgtt tcacagcgtc ccgtttgata aaggccggaa
     1621 gattgaagcg gtggaagcgg ctaagcttgg cacaaaggcg tctcacggct gtgtcagact
     1681 ttcgattgaa aatgcaaaat ggatgtatgt caacattcca agagggacaa aagtggtcat
     1741 tgaataaaac agggggtgca tcccgcctgc gcagttttag aatgagtgtt ctaaaaaaca
     1801 gaggagggcg gaatgatccg ccctcctctg tttgccgatt catgattgct gaaaagcata
     1861 agatgaaaac aaatttgcaa aaagcttaag actgcccgaa gccggcgctt tcactgtatg
     1921 gcgttacgat aaccaaaggc ggtgccggac ggcggttaaa cccgatggta ttcctcaacg
     1981 ccccggataa aggtccgctc aacggtcagg tcatcattca agaccagaat atcggcgttt
     2041 gacccgactg caagccggcc aacctgatg
//
LOCUS       DATALY010000368        24358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_255945,
            whole genome shotgun sequence.
ACCESSION   DATALY010000368 DATALY010000000
VERSION     DATALY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24358
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255945"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VHR42_09995"
     CDS             <1..997
                     /locus_tag="VHR42_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX3027535.1"
                     /translation="DLVDFGDFDGDGVNDIAIGWTQFTSGTDLRLSVYSLKAKTYKRV
                     YSGSFTTMKVLDINNDGKQDILLVEQDTVAKKSTARLVSYSCGRLSEIASVPLDSTVS
                     SYAELYETTVGKNDKGVLLDGYKGAHSMVTELVYWKNGSLQVPLYDKRSGTVISTFRD
                     DPVACSDLDEDGTVEIPFPVVMQGYEKAAYSKKLWTVRWADFAPATGSGSVFQTKFSC
                     VINSAEHYYFILPGDLVKKTGFAGNVTVDKKNGDNDWIFCQWDPKSKSEGRLLFAIRS
                     YTTQEWSSLTNKKSLNVIYQNDLTTYAAILGQKQGNDDPLYLTLDQIRNNFKIIS"
     gene            1071..1769
                     /locus_tag="VHR42_10000"
     CDS             1071..1769
                     /locus_tag="VHR42_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3027536.1"
                     /translation="MKKILVVEDEDAIREFVIINLKRAGYNVFEAGTGEDALKIYEEH
                     SGKFDVVLLDIMLPGIDGFAVCRKLREKSKSIGIIMLTARTQELDKVGGLMIGADDYV
                     TKPFSPSELVARIDALYRRLEMDKADSRKQDEIPSGPFLLNLKSRSLFKNDRQIVLTQ
                     VEYQIMKYFMENPDTALSRSDILNHVWGEDYFGELKIVDVNVRRLRMKIEDDPSNPNY
                     IMTVWGYGYKWNLK"
     gene            1873..3285
                     /locus_tag="VHR42_10005"
     CDS             1873..3285
                     /locus_tag="VHR42_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3027537.1"
                     /translation="MLGRGITKRWLINSMGVIFAILAVVWIFFALSIRGYYYSNVMTN
                     LRSRAQNNSSNFTRMIDTQNPDFYGTAYELVDSFLDKDKMELMILDKNGIVLATTSGF
                     TPADTKKYQDFLTASSSSSGIGEWNGVDDETGEKIMAVTSMLKDSDGNVMGASRYVVS
                     LSKIDMQIAIFVLISGIICLAIILFVVMSGTYFINSIVIPVRDIGATARRIASGDFEV
                     RIDKQYDDEIGELCDTINYMAGELGATDRMKNDFISSVSHELRTPLTAIKGWGETILS
                     SGPSDRETLSKGMTIIIDETERLSSMVEELLDFSRMQNGKFKLIMDKIDILAELGEAV
                     LMFSDRAHREGLMFIYNEPASLPPVMGDRNRLRQVFLNILDNAFKYSDPGGTVTVSTV
                     DNTDHLKVIISDTGCGISGYDLPKVKNKFFKANSTRRGSGIGLAVADEIVCLHGGTLE
                     LDSQEGKGTTVTITLPSYPK"
     gene            3315..4244
                     /locus_tag="VHR42_10010"
     CDS             3315..4244
                     /locus_tag="VHR42_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX3027538.1"
                     /translation="MAEKKHKTMIGGQAVIEGVMMKGPRSTAMAVRKTDGEISLETWE
                     NKDLGDRSPFFRIPLIRGVVNFVQMLRFGYQCLMKSAEIAGIEEEPSNLEQKVEKAFG
                     EKTLKALMSLSSLIGIVLAIVLFVVIPSVFISKTDVFLHYGAYKTLAEGLLKIGIFLL
                     YLFLISKISDIGRVFEYHGAEHKTISCYENGKELTVENARGCTRFHPRCGTSFILIVF
                     IVSILVFSFVTWSSLAVRILLKLLLLPFVVGVSYEIIKFAGRHDNSLMRFLLAPGLWL
                     QRMTTREPDDSQLEVAIQSLEAVLTGNPEDDGW"
     gene            4297..5175
                     /gene="prmC"
                     /locus_tag="VHR42_10015"
     CDS             4297..5175
                     /gene="prmC"
                     /locus_tag="VHR42_10015"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX3027539.1"
                     /translation="MTLRQAYQDAVRRLRNAGISDPAFDAACILEKNTGIKRHELPLK
                     GDTPAGDLAAFRSDVERRERREPLQYILGEWEFMGLRFSVGSGVLIPRPDTELLAGCA
                     IEELKTADRPAVLELCGGSGCVSVSIAKFVPGCRVYCLELSGQAISYLRGNLASNEVE
                     DRVKIIEGDMLNFEGCVGIPSPGSLDAVVCNPPYIRSEEIGTLQAEVGCYEPHLALDG
                     GPDGLLFYRAAQKWLTLLKAGGAAIFEVGAGQARDVTDILHQAGLNEIFVKKDYGGVE
                     RVVGGRMPAPKPKTQE"
     gene            5260..6333
                     /gene="recA"
                     /locus_tag="VHR42_10020"
     CDS             5260..6333
                     /gene="recA"
                     /locus_tag="VHR42_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX3027540.1"
                     /translation="MNDKKKALETALAGIEKQFGKGAVMKLGQNAAMNVGAISTGSLS
                     LDIALGIGGVPKGRIIEVYGPESSGKTTVALHMIAEAQKAGGEAAFIDAEHALDPVYA
                     KALGVDVDSLLVSQPDTGEQALEIAEALVRSGAIDIIVVDSVAALVPRAEIEGEMGDS
                     HVGLQARLMSQALRKLAGAISKSNCVAVFINQLREKVGVMYGSPEVTPGGRALKFYAS
                     VRIDVRKIESIKSGSDIIGTHTRAKVVKNKVAPPFREGEFDIMYGTGISKEGEIVDLG
                     VKLDIIQKSGSWFSYGEKRLAQGRDNVKELLRQNPELSEEIEKKVLENKDKLVTIKKI
                     PAREFKLAVPKPEAKIDVEADDI"
     gene            6337..6942
                     /locus_tag="VHR42_10025"
     CDS             6337..6942
                     /locus_tag="VHR42_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX3027541.1"
                     /translation="MLITRIKKLRSGRYGVYGDYRFLVSVDEEILVRRNLAEGREIDD
                     GQIQAIVEESVSSMAKDKALSLLSYRDHSKAELKNKLVRTVGEAAAWDAADKMEELGL
                     VNDADYAAKLARELFNKKFYGSARVTYEMLHRGIDREIVEAAVEELEIDPVEQILKFL
                     RRRYPAGISDEKNRKRASAALMRNGFQWDEIRQAFREYERI"
     gene            7010..8350
                     /gene="rimO"
                     /locus_tag="VHR42_10030"
     CDS             7010..8350
                     /gene="rimO"
                     /locus_tag="VHR42_10030"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX3027542.1"
                     /translation="MNIKVGLISLGCPKNQVDAETMLAGLSSAGFGITKDAGLADVVI
                     INTCGFIEDAKKESIENILEMAQLKKEGTIKGIVVTGCLAERYYKEMQQEFPEVNCIL
                     TVGRAGDIEQAVRAAFDGGSLFLDGKPEQLELGGKRVVTTLPFFAYLKIAEGCDNCCS
                     YCVIPKLRGRFRSRPMEDILDEARRLVGGGVRELTLVAQDTTRYGEDLYGRMMLPGLL
                     RELCKIEKLEWIRLLYCYPDKVTDELIDTIADEPKIAKYIDLPIQHVSTRIVRQMNRR
                     DDRAKLETLIGRLREKIPGLTLRTTLIVGFPGETQEEFAELIEFVKQIRFERLGAFAY
                     SREDGTPAAEMDGQIEEELKLRRQEIVMEEQSRINDELNQKMVGGAVKVLIEGFDRYA
                     ECYFGRSEADAPDIDGKIFVQSKQKLEPGTFQMVRISGVMDFDLLGEVVSESNQ"
     gene            8376..8915
                     /gene="pgsA"
                     /locus_tag="VHR42_10035"
     CDS             8376..8915
                     /gene="pgsA"
                     /locus_tag="VHR42_10035"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX3027543.1"
                     /translation="MNTPNKLTILRMLLVPVFLVLLLIESIPGHMFWAMLCFILASVT
                     DLIDGHLARKYDQITDFGKFLDPLADKLLVTAALVGFVQLGLSDSWVAMVIIAREFLV
                     TSLRLVAAGSGKVIAANIWGKAKTNSQIFAILLTMLGSLLRWPAGIGVAALWVAAALT
                     IISGIQYTWAYRNYIDTSK"
     gene            8991..9155
                     /locus_tag="VHR42_10040"
     CDS             8991..9155
                     /locus_tag="VHR42_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027544.1"
                     /translation="MHRHQRKSALAESALGGSSGKCAREHGAGKADAEYCAAEAPLSP
                     TREKSEWNRG"
     gene            9250..9918
                     /gene="epsC"
                     /locus_tag="VHR42_10045"
     CDS             9250..9918
                     /gene="epsC"
                     /locus_tag="VHR42_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX3027545.1"
                     /translation="MLQNLRYDIKAVRERDPAARTKLEVLLLYSGLHAMIAYRIAHWL
                     YGHKRYFLARWVSQHAKFKTGIEIHPGAKIGRGLFIDHGAGVVIGETTEIGDNCTLYQ
                     GVTLGGTGKDKGKRHPTLGDNVMVGSGAKILGPFLVGSNSKIGANALVLSEVPPDSTA
                     VGVPARVVRIRGKKVDCDCDLDQIHMPDPVSQEMCKLCMRIDELEKELKSLKKERNGA
                     DEDL"
     gene            9905..11305
                     /gene="cysS"
                     /locus_tag="VHR42_10050"
     CDS             9905..11305
                     /gene="cysS"
                     /locus_tag="VHR42_10050"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX3027546.1"
                     /translation="MKIYNTLTRKKEEFLPLEPGKAKIYACGPTVYNFIHIGNARPLI
                     VFDILRRYLEYRGFEVTFVQNFTDVDDKMINRANSEGVTVAEIAEKYIGEYRRDAQGL
                     NVREATIHPRATENMDEIIDLIRTLIDRGYAYESGGDVYFRARRFKQYGKLSHQPLED
                     LEAGARIDISEVKEDPMDFALWKTAKSGEPMWESPWGKGRPGWHIECSAMARRFLGKT
                     IDIHCGGQDLIFPHHENEIAQSECANSAEFAHYWMHNGYINVDNRKMSKSLNNFFTVR
                     DVAERYGYEPIRFLMLSAHYRSPINYNVEIIEQSKSALERIYQCRENIDFVSASLPEK
                     NIQGDAVMKETLLKRKEQFIRAMDDDLNTADAISAIFDLVKDINVRLGSDVKASKPGL
                     TVAAALIDELCGVLGLLYKKEAKSLDAEIEELIRKRNQARKNKDFKEADKIRDELKNR
                     GIKLEDSPQGVKWSRV"
     gene            complement(11994..12500)
                     /locus_tag="VHR42_10055"
     CDS             complement(11994..12500)
                     /locus_tag="VHR42_10055"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3027547.1"
                     /translation="MSEFSSIKPEQLQGNVFDKIGNQWMLITAGDGNSFNTMTASWGG
                     MGVLWSRPVAFAFIRPTRYTYEFVEKSDRFSLSFYDENYREALNLCGTKSGRDLDKAK
                     ATGLTPVEAEKTVYFEQAKLVLICKKLYSDDFKQQNFIDRSVDEQMYHGDYHRMYIGE
                     IEKILIKK"
     gene            12719..13396
                     /locus_tag="VHR42_10060"
     CDS             12719..13396
                     /locus_tag="VHR42_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX3027548.1"
                     /translation="MRENIPEFEGSLRKHMLKVPESIYKASGILIFGKRIRSLVFTTD
                     VCIIRNINADAVIAVYPFTPQPIITQAIMLTADIPVFCGVGGGITQGKRVVNVALHAE
                     FQGAIGVVVNAPTSNDVIAAVRETIDIPVVVTVVSSQDDIGARLEAGASIINVSGGAA
                     TPEIVAEIRSRFPVLPIIATGGPDEKTISRTIRAGANAITWTPPSGSEILGRIMESYR
                     EEEPSGG"
     gene            13620..14837
                     /locus_tag="VHR42_10065"
     CDS             13620..14837
                     /locus_tag="VHR42_10065"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX3027549.1"
                     /translation="MKEIKKVVLAYSGGLDTSIIIPWLKENYGCEVIAVAGDVGQGKE
                     LSGLKEKAIKTGASKIYIEDLTKEFIEDFIWPTLKADAKYEDYLLGTSFARPIIAKRL
                     VEIAKAEGADAIVHGCTGKGNDQVRFELTIKAFAPEMEIIAPWRIWDLKSRDEEIDYA
                     EAHHIPLKITRETNYSKDMNLWHLSHEGLDLEDPANEPMYDKILELGVSPEKAPDHPT
                     YVTIGFEKGIPVSIDGVKTGSVEMIQKLNKLGGENGIGILDIVENRLVGMKSRGIYET
                     PGGEILYHAHKTLETLTLDKETFHFKEIIGQKFAEVVYNGQWYTPLREALSAFTDKTQ
                     ENVTGEVKLKLYKGNIINAGTTSPYSLYSEEVATFGEDEVYNQKDSEGFINLFGLPIK
                     VQAMLQKSGAESK"
     gene            14980..17412
                     /gene="argH"
                     /locus_tag="VHR42_10070"
     CDS             14980..17412
                     /gene="argH"
                     /locus_tag="VHR42_10070"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX3027550.1"
                     /translation="MWAGRFSKETDSRVNDFNSSISFDCRMFREDIGGSMAHAEMLGK
                     CGIIQKEESIAIIAGLKEILQDIENGRLLFDPDAEDIHMFVEAELTRRIGSAGKRLHT
                     ARSRNDQVALDLRLYLRAEITEIKAALSGLIQTLAKKADENLTAIMPGYTHLQRAQPV
                     TFAHHLLAYAQMFARDLTRLSDCAKRMNCSPLGCGALATTTYSIDRSMTADLLGFSAP
                     TANSLDGVSDRDFVIELAGALSIVMMHLSRFSEEIVLWCSWEFKFIELDDAYATGSSI
                     MPQKKNPDVAELVRGKTGRVYGDLIALLTVMKGLPLAYNKDMQEDKEAVFDAIDQVKL
                     CVSTFAPMVETMTVLKDHMRQAASKGFINATDCAYYLTKKGIPFRDAYKITGSLVAYC
                     IEKGKTLEVLELAEYRKFCSEFDEGVYQAISLDRCVSGRSVQGGPAPGVGETAAETNQ
                     CAAGGVPKTGFYRGIISISFLKTRAFRRRFTGCAIWRGIIMKFKIFVDGQEGTTGLKI
                     YERLGLQEQIDVLAITPALRKDPSERRRLLNQADVAFLCLPDAASREAVSMIENENTR
                     VIDSSTAFRCDPGWVYGFPELDRSQRDKIIHAKRVAVPGCHATGFHSIVHPLVTGGLI
                     ETSYPLTCHSVSGYSGGGKKLIARYEQVQTGDSGHEQSLRGLRYYALGLRHKHLAEMR
                     YVNGLDRPPLFTPSVGDFSRGMLVTVPLWKNQMKKPMGAAEVRDYYAEYYQGEKFIRV
                     MPFDSERFLEDGFLDAMGCNGTNSLELFVFGHDDQMFIVSRLDNLGKGASGAAVQCMN
                     LMLGQREDAGLF"
     gene            17442..18656
                     /gene="argJ"
                     /locus_tag="VHR42_10075"
     CDS             17442..18656
                     /gene="argJ"
                     /locus_tag="VHR42_10075"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX3027551.1"
                     /translation="MKYIDGSVTAPLGFQASGMHCGIRKNRSKKDLALIFSQQECDAA
                     AVYTQNKVKGAPITVTKQHLANGKARAVICNSGNANTCNADGEEIADLMCKHTARVLG
                     ISEDDVIVASTGVIGQPLPVEPIISSLPKLAGLLADDGAAQAAEAIMTTDTRKKEVAV
                     EFLLNGKPCRIGGIAKGSGMIHPNMATMLCFLTTDADIDSGLLAQALTASVMKSFNMV
                     SIDGDTSTNDMVSILASGLCGSGRIVSADESYQTFCGALDEITISLAREVARDGEGAT
                     KLLECRVTGAKTEQAAKAVAKSVIGSSLFKAAMFGADANWGRVLCAVGYADADLDIGK
                     VDVAFESENGLIEVCKCGAGLDFSEEKARSVLIHDEIVIHISLGDGEFEAAAWGCDLT
                     YDYVKINGDYRT"
     gene            18689..19552
                     /gene="argB"
                     /locus_tag="VHR42_10080"
     CDS             18689..19552
                     /gene="argB"
                     /locus_tag="VHR42_10080"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX3027552.1"
                     /translation="MNQVSDHYTARVLVHALPYIQEYNNKTVVVKYGGNAMINRELKD
                     AVMTDLVLLSLVGIKIVLVHGGGPEITEMLEKIGKASLFVGGLRYTDAETMDIVKMVL
                     AGKVNKELVQSLEMKDGHALGLCGLDGGMIRAEKLEPGGVDLGYVGKIVSVDVTPIND
                     ALSKGYIPVIATMGAGRDGSVYNINADTAAARIASDIGAEKLILLTDTRGLLRDREDE
                     ESLISLIHVSDVPGLVNENIISGGMIPKVDCCVEAVRRGVKRAHIIDGRIPHSILVEM
                     FSDEGIGTMFQ"
     gene            19626..20777
                     /locus_tag="VHR42_10085"
     CDS             19626..20777
                     /locus_tag="VHR42_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3027553.1"
                     /translation="MPTYTRFNVALRSGKGATAVGVDGKKYLDFGSGIGVNALGYSDE
                     GWVRAVSGQAALLSHTSNLYYSPVQAELAGLLCSLTGFGAAFLANSGAEANEGMIKLA
                     RKYSFDRYGDGRSVVVTLKNSFHGRTVTTLSATGQEEFHRYFFPFTCGFRFAEPNDFG
                     SVKEACKGDVCGILLEAVQGEGGVYPLDPDFVRQTAEFAKENDILLMFDEVQTGVGRT
                     GRLFAYEHYGVSPDAISSAKALGGGLPMGAVLCSERLKEVMGPGSHGSTFGGNPIACA
                     GAIEVLSRVSKPEFLIKVNEKALYFRKKLAEIKDVARIRGLGLMIGFEVDGVDSKQAA
                     AKLSENGLLVLTAKKALRLLPPLVITKDEIDRGIEILKTVFAELKHTEG"
     gene            20782..21687
                     /gene="argF"
                     /locus_tag="VHR42_10090"
     CDS             20782..21687
                     /gene="argF"
                     /locus_tag="VHR42_10090"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX3027554.1"
                     /translation="MKHLLKLLDLSKEEILDILNLGDQLKYEKKHGISHPRLAGKTLG
                     MIFEKASTRTRVSFETGMYQLGGNALFLSSKESQIGRGEPIQDTARVLSRYLNGIMIR
                     TFGQKEVEDLAAYGSIPIINGLTDFAHPCQVLADLMTIREFKGKLDGLKLCFIGDGNN
                     MANSLIVGGLKVGMEVSVACPEGYDPDPVVLEFAKTFGDKFRLCRDPEKAAGGADAVF
                     TDVWASMGQESESQKRKLKFDGYQVNDKIIAAANPGTMVQHCLPAHRGEEITEKVFEA
                     HADEIFEEAENRLHAQKAVLVRLME"
     gene            21879..22310
                     /locus_tag="VHR42_10095"
     CDS             21879..22310
                     /locus_tag="VHR42_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX3027555.1"
                     /translation="MTGWLMFWLVAVILLAIIEIATTQLVAIWMAVGGVGALVACSFN
                     ASVLTQFLIFIAISAALLLLTRPMVKKFLTVKQVRTNADRLIGGIATVTEEIVNDESR
                     GEAKIGGVTWMARSLDGEKIPPDQKVQVERIEGAKLIVSKL"
     gene            22330..23292
                     /locus_tag="VHR42_10100"
     CDS             22330..23292
                     /locus_tag="VHR42_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3027556.1"
                     /translation="MPFTTVVWLVLAVLVIILLVSCFRIVPQAHAYVIERLGAYHTTW
                     GSGSIKFKAPFVDRIAKVISLKEQVVDFPPQPVITKDNVTMQIDTIVFFQVTDPKLYT
                     YGVERPLQAIENLTATTLRNIIGDLELDHTLTSRDVINTKIRTLLDEASDPWGIKVNR
                     VELKNIVPPREIQDAMEKQMKAERERRQSVLRAEGEKTSQILVSEGQKEAQILQAEAQ
                     KQAAILHAEGVKEAKIREAEGEAAAILKVQEAVAEGIKLLNVSSPTDQVIALKSLDTL
                     AKVADGKATKIIIPSELQSIASLAVSVKELVTGDKAEQQETQNS"
     gene            23643..24149
                     /gene="purE"
                     /locus_tag="VHR42_10105"
     CDS             23643..24149
                     /gene="purE"
                     /locus_tag="VHR42_10105"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX3027557.1"
                     /translation="MSESKKVAVIMGSDSDLPVLKGCIRTLKELGIPAEVHVMSAHRT
                     PDRAAEFSKNAIRNGFGVIIAAAGKAAHLAGVLAAGTTLPVIGVPVKSSTLDGLDALL
                     STVQMPKGIPVATVAIDGADNAAILAAQILAVSDQGLAEKLAEMKKNMADGVMKKDQN
                     IQVEVEKI"
     gene            24146..>24358
                     /locus_tag="VHR42_10110"
     CDS             24146..>24358
                     /locus_tag="VHR42_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX3027558.1"
                     /translation="MKKLEQVYEGKAKKVFKTDDPNLFIVDYKDDATAFNGLKKGTIQ
                     NKGIINNRVTNYLMKMLESKGIHTHFV"
BASE COUNT         6286 a         5482 c         6849 g         5741 t
ORIGIN      
        1 ggatctggtt gacttcggtg attttgacgg tgacggtgtc aacgatattg ccattggctg
       61 gacacagttt accagcggca ccgacctgag gctgagtgtt tattctctga aagccaagac
      121 ttataaaagg gtttacagcg gcagttttac cacaatgaaa gttctggata ttaacaatga
      181 tgggaaacag gatatccttc tcgtcgagca ggataccgtt gcaaaaaagt ctacggcaag
      241 gctcgtgagt tattcctgtg ggcgtctttc tgaaattgct tctgttccgc ttgacagtac
      301 ggtgagcagt tacgcggaac tgtatgagac cacagtgggg aaaaacgaca agggcgtgtt
      361 gcttgacggg tataaaggcg cccattccat ggtgactgag ctggtttact ggaaaaatgg
      421 gagtcttcag gtgccgcttt atgacaaacg aagcggaacc gttatctcta cattccggga
      481 tgatccggta gcctgcagcg atctggatga agacggtacg gtggagattc cgtttccggt
      541 tgtaatgcag ggttatgaga aagccgcata cagtaaaaaa ctatggactg tacgatgggc
      601 tgattttgcc ccggcaaccg gttccggaag cgtttttcag accaaatttt cctgcgtgat
      661 caacagcgcc gagcattatt actttatact gccgggggac ctggtgaaaa aaacaggttt
      721 tgcaggcaat gtcacagttg acaaaaaaaa tggcgacaac gactggatat tctgtcagtg
      781 ggatccgaaa agcaaatccg aaggcaggct tctgtttgcc atccgctcct acacgacgca
      841 ggaatggagt tctcttacca acaaaaaatc gctcaatgtg atataccaaa atgatctgac
      901 cacctatgcc gccatactgg ggcagaagca gggtaatgac gacccgcttt atcttaccct
      961 ggatcagatc agaaacaact ttaagattat tagctgagcc gtctgaaatc cggggcgaac
     1021 cggactgcgg gggcgtacag aatttatttg aaccgggagg gattgctatg ctgaagaaga
     1081 ttctggtggt ggaagatgaa gacgccatca gggaatttgt tattatcaac ctcaaacgcg
     1141 ccggttacaa tgtttttgaa gcgggcaccg gcgaggatgc gcttaaaata tatgaagagc
     1201 acagcggaaa atttgatgtc gtgctgctgg atattatgct tcccggaatc gacgggtttg
     1261 ccgtttgcag aaagcttcgg gaaaagagca agtcgattgg gatcattatg ctgaccgcac
     1321 gcacacagga gcttgacaag gtcggaggat tgatgatcgg cgccgacgac tacgtcacca
     1381 agcctttcag cccgtcggag cttgtggcgc gcatcgacgc gctttaccgc agactcgaga
     1441 tggataaggc cgacagccgt aaacaggacg aaataccatc gggcccgttc ctgctcaatc
     1501 tcaaaagccg gtctcttttt aaaaacgacc ggcagattgt gctcacgcag gtggaatatc
     1561 aaattatgaa atattttatg gaaaatccgg ataccgcgct cagccggagt gacattctca
     1621 atcatgtctg gggcgaggat tatttcggcg agcttaaaat tgtcgatgtg aatgtccggc
     1681 gtctccggat gaagattgaa gacgaccctt ccaacccgaa ttatatcatg acagtctggg
     1741 gatatggcta caaatggaat ttaaagtaaa ttgcctttcc gccggggacg aacccttccg
     1801 cttttcagaa atgcggatgc gcttgatttt ttaacagaca caccagttcg cggaaaggag
     1861 gattctggac ggatgttagg cagaggaatt acaaaaagat ggctgatcaa cagtatgggc
     1921 gtaatttttg ccatacttgc tgttgtctgg atttttttcg cgctttcgat caggggatat
     1981 tattacagca atgtcatgac aaacttaaga tcccgggcgc agaacaacag cagcaatttt
     2041 acccggatga ttgacactca gaatcccgat ttttacggaa ccgcttatga gctggttgac
     2101 agttttcttg ataaagacaa aatggaactg atgatcctgg ataaaaacgg gattgtgctg
     2161 gcgacaacca gcgggttcac cccggccgat accaaaaagt atcaggattt tctgaccgct
     2221 tcctcgtcct cgtcgggcat cggcgaatgg aacggggttg acgatgaaac cggcgaaaaa
     2281 attatggctg ttacctccat gctgaaggat tcggacggaa atgtcatggg cgcgtcgagg
     2341 tatgttgttt cgctgtccaa aattgacatg cagatcgcga tttttgtgct gatcagcgga
     2401 attatctgtc ttgccatcat tcttttcgtg gtgatgtccg gaacctattt tatcaattcg
     2461 attgttattc ccgtcaggga tatcggcgcc acggccagaa gaattgcgtc cggcgatttt
     2521 gaagtcagaa tcgacaaaca atatgatgat gaaatcggtg aattgtgcga tacgatcaat
     2581 tatatggcgg gggaactcgg cgccaccgac cggatgaaaa acgatttcat ttcatctgtt
     2641 tcacacgaat tgcgcacgcc gcttaccgcc attaagggat ggggcgaaac gatcctttcc
     2701 tccggcccgt cggacaggga gactctttcc aagggcatga cgatcattat tgatgaaacc
     2761 gagcgtcttt cttctatggt ggaagagctt ttggatttct ccagaatgca aaacggaaaa
     2821 ttcaaactga tcatggataa aatcgacatt ctggctgagt tgggcgaagc cgtgctgatg
     2881 ttttccgaca gagcccatcg ggaaggcctg atgtttatct ataacgagcc tgcctccctt
     2941 ccgcccgtta tgggcgaccg gaaccggctc agacaggttt ttctcaatat tctcgataac
     3001 gcttttaaat attccgatcc gggcggaacg gttacggttt caacagtgga caataccgat
     3061 catttaaaag tcataatcag tgacaccggc tgtggaattt cgggatacga cctgcctaag
     3121 gttaaaaata aattcttcaa ggccaattcc acccggcgcg ggtcgggcat cggtcttgcc
     3181 gtagcggacg aaatcgtctg tctgcatggg ggaacactgg aactcgacag ccaggagggt
     3241 aagggcacaa ccgtgacgat tacgctgccc tcatatccga aatagacctt tgcaagtgaa
     3301 ggagtgttgc cgttttggca gagaaaaagc acaaaaccat gattggcggc caggcggtaa
     3361 tcgagggcgt gatgatgaaa ggtccccgca gtaccgcaat ggcggtcaga aagacggacg
     3421 gggaaatcag ccttgaaacc tgggaaaata aagatctcgg agaccggagc ccctttttcc
     3481 gaataccgct tattcgggga gtggtcaatt ttgttcaaat gctccgtttc ggttatcagt
     3541 gcctgatgaa atcggctgaa attgccggaa tcgaagagga accaagcaat ttggaacaaa
     3601 aggttgagaa ggcttttggt gaaaaaacgt taaaggctct gatgagcctg tcttcactga
     3661 tcgggatcgt tctggccatt gtcctttttg tggtgattcc atcggttttt atttcgaaaa
     3721 ccgatgtttt tcttcattat ggcgcctata aaacattggc ggaaggtctg ctgaaaatcg
     3781 gtatcttcct gctttacctg tttctgattt ccaaaatcag tgacatcggg agagtttttg
     3841 aataccatgg ggccgagcat aaaacaatca gctgctatga aaacggaaaa gagcttacgg
     3901 tagaaaatgc aaggggatgc acccggtttc accccagatg cgggacaagc ttcatcctga
     3961 tcgtttttat tgtaagtatt ttggtctttt cgtttgtaac ctggagcagt ttggcggttc
     4021 ggattcttct gaaattgctt ctgctgcctt tcgtagtcgg cgtgtcttat gaaattatca
     4081 aattcgccgg caggcacgac aattccctga tgagattcct tttggccccg ggcctgtggc
     4141 tgcagcgcat gacaacaagg gaaccggacg acagtcagct ggaggtcgcc atccaatcgc
     4201 ttgaagctgt tttaaccgga aatccggagg acgacggctg gtaacccgat tttgaatgaa
     4261 ttccggcgtt taggggccgg tctggaggca aaaggaatga cgctcaggca ggcatatcag
     4321 gacgcagtca ggcggctcag aaacgccgga atatcagatc ccgcgtttga tgccgcctgt
     4381 attcttgaga agaatacggg aataaaaagg catgagctgc cgctgaaagg cgacacgccg
     4441 gccggggatt tggccgcttt ccgctcggat gtggaaagaa gggagagacg ggagccgctt
     4501 caatatattc tcggtgagtg ggagtttatg ggcctgcgtt tttcggtcgg cagcggagtg
     4561 ctgattccga ggccggatac cgagttgctg gccggctgcg cgattgagga gttaaagacg
     4621 gccgaccggc cggccgtgct ggagctttgc ggcgggagcg gctgcgtctc ggtgtccatc
     4681 gcaaaatttg ttccggggtg tcgggtctat tgcctggaat tgtccggaca ggcgatttcc
     4741 tatcttcggg ggaatcttgc ttccaatgag gtggaggacc gggtaaagat tattgaaggc
     4801 gatatgctta attttgaagg ctgtgtcggc attccatcgc ccgggagcct tgacgccgtc
     4861 gtttgcaatc ctccttacat ccgttcggag gaaatcggaa ccctccaggc ggaggtcggc
     4921 tgttatgaac cgcatctggc tttggatgga gggccggacg gccttttatt ttaccgggcg
     4981 gctcaaaagt ggctgaccct tctgaaagcg ggaggagccg caatttttga agtcggggcc
     5041 ggccaggcac gggacgtgac ggatattctt catcaagccg gtctaaatga aatttttgtg
     5101 aaaaaggatt atggcggcgt cgagagagtc gtaggcggcc ggatgcctgc tccgaagccg
     5161 aaaacgcaag aataagattg cttttaaaaa tgcgatcagt tataataaat ctggctaaat
     5221 caatgaacaa aagaaatttt tatggagggg gctgcctgaa tgaatgataa gaaaaaggct
     5281 cttgaaacag cgctggccgg catagaaaaa cagtttggca aaggcgcggt tatgaaactg
     5341 ggacagaacg ccgcgatgaa tgtgggagcc atttccaccg gttcgctttc ccttgacatt
     5401 gctcttggaa tcggcggtgt gccaaagggc aggatcattg aagtctacgg ccctgaatcc
     5461 tccggtaaaa caacggttgc cctgcatatg atcgcggaag cgcagaaagc aggaggagag
     5521 gccgctttta tagacgcaga gcacgccctc gacccggttt atgcaaaggc gcttggcgtt
     5581 gatgtagatt cgctgctggt gtcgcagcct gataccggcg agcaggctct tgaaatcgcc
     5641 gaggcacttg tccgaagcgg tgcaattgac attattgtcg tcgactcggt ggcggcgctg
     5701 gttccccggg ctgaaatcga aggggaaatg ggcgattccc atgtgggtct tcaggcgaga
     5761 ctgatgtctc aggctttgag aaaactggcg ggagccattt ccaaatcgaa ctgcgtagcg
     5821 gtctttatca atcagctccg tgaaaaagtg ggcgttatgt atggcagccc cgaggtgact
     5881 ccgggcggcc gggcactgaa attttatgcg tcggtcagaa tcgatgtgcg aaaaatagaa
     5941 tccatcaaat ccggttcgga tatcatcggt actcatacca gagccaaggt tgtgaaaaac
     6001 aaggttgctc cgcctttccg cgagggagaa ttcgatatca tgtacggtac gggcatctca
     6061 aaggaaggcg agattgttga ccttggagtc aaactggata ttattcagaa gagcggctca
     6121 tggttctcct acggtgaaaa acgactggca cagggaagag acaatgtcaa agaacttctc
     6181 cgtcagaatc ctgagctttc tgaggaaatc gagaagaaag tgttggaaaa caaggacaag
     6241 cttgtgacca tcaagaaaat accggcaagg gagtttaaat tggctgtccc taagccggag
     6301 gcaaaaatcg acgtggaggc ggatgatatc taatcaatgc tgatcaccag aattaaaaaa
     6361 ctgcggagcg gccggtatgg tgtttatggc gattaccggt tccttgtttc cgtggatgag
     6421 gagatccttg tgcgccgcaa tcttgcagaa ggccgcgaga ttgacgacgg ccagattcag
     6481 gccattgtcg aagagtcggt atcctccatg gcaaaagaca aggccctgag cctgctgtcc
     6541 tatcgggacc attccaaggc tgaacttaaa aataaacttg tcaggacggt aggggaggcg
     6601 gcggcatggg atgccgcgga taaaatggag gagctggggc tcgtcaacga cgccgattat
     6661 gccgccaagc ttgcccgcga acttttcaac aaaaaatttt atggctcggc ccgggtgacc
     6721 tatgaaatgc tgcaccgcgg aatcgaccgc gaaattgtcg aggccgctgt ggaagaattg
     6781 gaaatcgacc cggttgagca gatcctgaaa tttttgagaa gacggtaccc tgccggtatc
     6841 agcgacgaaa aaaacagaaa aagggcctcc gccgctctga tgcgtaacgg atttcagtgg
     6901 gacgagatac ggcaggcctt cagggaatat gaaagaatat aatttgtttc tccgacggca
     6961 aaccgttgcg gcggaggaat agtttaaaca ggatggatgg agtatggcga tgaatataaa
     7021 agtcggcctg atttcgctgg gctgtcccaa aaaccaggtg gacgccgaga cgatgctggc
     7081 aggtctcagt tcagcgggtt tcggaatcac aaaagacgcc ggcctggcgg atgttgtaat
     7141 cattaatacc tgtggattta tcgaagacgc gaaaaaggaa tccatcgaga atattttaga
     7201 aatggcccag ctcaaaaagg aaggcacgat aaaaggcatt gtcgtaacag gctgccttgc
     7261 cgagcgttac tataaagaga tgcagcagga atttcccgag gttaactgta ttctgaccgt
     7321 tgggagagcg ggagatatcg agcaggcggt cagagctgcc tttgacggcg gcagcctgtt
     7381 tcttgacgga aagccggagc agctcgaact gggcggaaaa agggtcgtca cgacactgcc
     7441 gtttttcgcc tatctgaaga ttgccgaggg ctgtgacaac tgttgctctt actgcgttat
     7501 tccaaagctc cgcggtcggt ttcggagcag gccgatggag gacatattgg acgaggcccg
     7561 ccggcttgtc ggcggcggcg ttcgggagct gacccttgtt gcgcaggata cgacgcgata
     7621 cggggaagat ctttacggcc ggatgatgct gcccgggctg ctcagggagc tttgcaaaat
     7681 cgagaagctg gaatggatcc ggctgcttta ctgttatccc gacaaggtga ccgatgagct
     7741 gatcgacacg attgccgatg agcccaaaat tgcaaaatat attgacctgc ccattcagca
     7801 tgtcagcacg aggatcgtcc gtcagatgaa cagacgggat gaccgcgcca agctggagac
     7861 gctgattggc aggcttcgcg aaaaaattcc gggccttacg ctgcgcacga cgctgattgt
     7921 cggtttcccg ggggaaacgc aggaggagtt tgctgagctt atagagtttg tcaaacagat
     7981 ccggttcgag cgtttgggcg cttttgccta ttcccgcgag gacggcacgc cggcagcgga
     8041 aatggatggt cagattgaag aagaattaaa actgcgcagg caggaaatag taatggaaga
     8101 gcagtcgcgc atcaacgacg aactcaatca gaagatggtc ggcggtgcgg taaaagtcct
     8161 gatcgagggc tttgaccgtt atgcggagtg ctattttggc aggagcgagg cggacgcgcc
     8221 ggatattgac ggcaaaattt ttgtgcagtc gaaacagaaa cttgaacccg ggacattcca
     8281 aatggtcaga atcagcggcg ttatggactt tgaccttctg ggcgaggtgg tctcggaatc
     8341 aaatcagtaa tagcgggacg gaggactata atcggatgaa tacccctaac aagctgacaa
     8401 tacttcgaat gctgcttgtt ccagtttttt tggtgctgct gcttattgaa tcaataccgg
     8461 gccatatgtt ttgggccatg ctctgtttta ttctggcgtc ggtaaccgat ttgattgacg
     8521 ggcatctggc acgcaaatat gaccagatta ccgacttcgg aaaatttttg gaccctcttg
     8581 cagacaagct tctggtcacg gcggctctgg tcgggtttgt gcagcttggt ctttccgact
     8641 cgtgggtggc gatggtgatc atcgcgcgtg agttcctggt aacatccctg cggcttgtgg
     8701 cggcgggaag cggtaaggtg atcgctgcga atatctgggg aaaggcaaaa accaacagcc
     8761 agatttttgc gattcttctt acaatgctcg gcagtttgct ccggtggccg gcaggcattg
     8821 gtgtcgcggc cctttgggta gcggccgctc tgacgataat ttccgggatt caatatacct
     8881 gggcttatcg gaactatatc gacacgtcaa aataaaccat tgcgggactg agccgcatat
     8941 gataatatta ttacagcgaa tgaaatgcag cgaccgggaa aagtatccga atgcaccgtc
     9001 atcagagaaa gagcgcgctg gctgaaagcg ctctgggcgg atcatcgggg aaatgcgccc
     9061 gcgagcacgg cgccggaaag gctgatgccg agtattgcgc tgcggaggca ccgttatcgc
     9121 cgacgagaga aaaatcggag tggaaccgcg gataagtccg cctccggcct gcgggccggg
     9181 ggcggttttt gatttttgta aaaacctttc tggccttaag gtaaaaccaa atcaaaggga
     9241 ggcaagaaga tgctccaaaa tttacgatat gatattaaag cggtcagaga gcgcgacccg
     9301 gcggcccgga caaagctgga agtgctgctt ctttactcgg gtctgcacgc gatgatcgcc
     9361 taccggatcg ctcactggct ttacggccat aaacgctatt tccttgcccg atgggtatcg
     9421 cagcatgcca aatttaaaac cggcatagag attcaccccg gtgcgaaaat cgggaggggg
     9481 ctgtttattg accacggagc cggcgtcgtg atcggtgaaa ccaccgagat cggcgataac
     9541 tgcaccctct atcagggcgt gaccctcggg gggacgggta aggataaggg aaaacgccac
     9601 ccgacactgg gcgataacgt catggtaggt tccggcgcga agattctcgg cccttttctg
     9661 gtaggaagca attccaaaat cggggcgaac gcgcttgtac tgtcggaagt gcctccggat
     9721 tccaccgcgg ttggtgttcc ggcacgggtt gtccggatca gggggaaaaa ggttgactgt
     9781 gactgtgacc tggatcagat ccatatgccc gacccggtat ctcaggaaat gtgcaaactc
     9841 tgcatgagaa tcgacgagct cgaaaaagag ttgaaatcgc ttaaaaagga gaggaatggc
     9901 gcagatgaag atttataata cgctgacaag gaaaaaagag gaattcttac cgcttgaacc
     9961 cggaaaagcg aaaatttacg cctgcgggcc gaccgtctat aactttatcc atatcggaaa
    10021 cgccagaccg ctgattgtct ttgatatttt aagacgttat ctggaatacc gcggctttga
    10081 agttacgttt gttcagaatt tcactgatgt tgacgacaaa atgattaacc gggccaacag
    10141 cgagggcgtc acagtagcgg agattgccga aaaatatatc ggggaatacc gcagggacgc
    10201 gcagggcctg aatgtccggg aagccaccat ccatccccgt gcaactgaaa atatggatga
    10261 aatcattgat ttgatcagga ctctgattga ccgaggatac gcctatgaat ccggcggcga
    10321 cgtctatttc agagccagac ggtttaaaca atacggcaag ctttcacatc agccccttga
    10381 agatctggag gcgggtgcga gaattgatat ctcagaggtc aaggaagacc cgatggactt
    10441 tgccctttgg aaaacagcca aatcgggcga gccgatgtgg gagtccccct ggggaaaagg
    10501 aaggccggga tggcacattg aatgctcggc aatggccaga aggtttctcg gaaaaaccat
    10561 cgatatccac tgcggagggc aggatttgat ttttccgcat catgagaacg aaatcgcaca
    10621 gagtgaatgc gccaattccg cagaattcgc ccattactgg atgcataacg gctatattaa
    10681 cgtcgataac cgcaaaatgt cgaaatccct taacaatttt ttcactgtcc gcgatgtcgc
    10741 ggaacggtat ggctatgagc caatccgttt tctgatgctt tctgcccatt acagaagtcc
    10801 tatcaattac aatgttgaga tcattgaaca gtcgaaatcc gcgcttgaac ggatctatca
    10861 atgccgggaa aatatcgact tcgtatcagc ctccctcccg gaaaagaata ttcagggcga
    10921 tgcggtgatg aaggagaccc tgctcaagcg taaagagcag tttatccggg caatggatga
    10981 tgatctaaat accgccgacg ctatttccgc tatttttgat ctggtaaagg acattaatgt
    11041 ccgtctgggt tctgatgtga aggcatccaa acccggtctg acggtagccg ccgctttgat
    11101 agacgagctt tgcggtgtcc ttggtttgct ttataagaaa gaagccaaga gtctggacgc
    11161 tgaaattgaa gaactgattc gcaaaagaaa ccaggcgcga aaaaacaagg attttaaaga
    11221 ggccgacaag atccgggacg agctcaaaaa ccgggggatt aagcttgaag acagcccgca
    11281 gggcgtgaaa tggtcgagag tttaaaccct gattgattta aactggttgc tgccgaaccg
    11341 ccgctctgga gaaacagggc ggcggtttct ctgttttgtc tgtgcatccg agtttagtgt
    11401 tcattcgggt tccggatata atttgaagta acatggtaaa tatacatata taatttttat
    11461 aatgtttaaa aaatatattg acattgcgta gatgctgtgc tattctaaag ccagagaaca
    11521 caagaaagta agaaagtaag aaagggaggc gattccaatg ggctggttga ttcccgggaa
    11581 aacttcagat ttttatggcg aggactgcca ggccgaacat cattaaattg cgggagagtt
    11641 cgatcaatga cctcccgaag cctgcgactt ttcatgcaag atggggcttt ctgtcccgcc
    11701 agttttgaga acgaggcaaa aacaggatcc cggtgttttt gaatgacatc acttgataat
    11761 ttttgctgat ttaacacaat gcagcgttta gagggctcat ctccctcgat ttaaagcgca
    11821 ttccacggat tcttccacaa tcagccacca tggagtggaa aagtgagagg caggtgaacc
    11881 acatttctgt tcccgggtag ccgggagatc atccggtatg cagatccgga atgtttcgaa
    11941 aaacaaaaat ctgatcaaaa aggggccgta ccagatggta cggccctaat tgattatttt
    12001 tttatcagga ttttttcgat ttcaccgata tacatccggt ggtaatcacc atgatacatt
    12061 tgctcatcaa cagaccggtc gatgaagttc tgctgtttaa aatcatcgga gtaaagcttt
    12121 ttgcagatga ggacgagctt tgcctgctca aagtatacgg ttttttccgc ttcgaccgga
    12181 gtgaggccgg tcgctttggc tttgtcgaga tctctgcccg atttggtgcc gcacagattc
    12241 agggcctccc ggtaattttc atcataaaag ctgagggaga aacgatcact tttttcaaca
    12301 aactcatagg tgtagcgggt tgggcggata aaagcgaatg ccacgggcct gctccagaga
    12361 acccccattc cgccccagct ggctgtcatt gtattaaagg aatttccgtc gccggccgta
    12421 ataagcatcc attgatttcc gattttgtca aagacatttc cctgaagctg ttccggtttg
    12481 attgaagaaa actctgacat aaaaccaagc ctcctaattt tgtatggtta ttcttttttt
    12541 attataagct ttttgtctgc ttttttaaat aatttggaaa gaatattaaa attgttccct
    12601 gcgaccgatt cgtaggcagc ggccttgaac ttattaaagt ttataaaata cgactttatt
    12661 tcggaaaata tctgtattat aatatatgga aatatataca agtcaaggaa gtggtattat
    12721 gcgggaaaac attcctgaat ttgaaggcag cctaagaaaa catatgctta aagtgccgga
    12781 aagtatttat aaagcgtcgg gaatattaat attcgggaaa cgaatccgat cgttggtttt
    12841 tacgacggat gtctgcatta tccgcaacat aaacgccgac gctgtcattg ccgtttatcc
    12901 ttttacgccc cagccgatca ttacgcaggc catcatgctc actgccgata ttcctgtttt
    12961 ctgcggagtc ggcgggggca ttacacaagg caaacgggtt gtaaacgtcg cgctgcatgc
    13021 agaatttcaa ggggcaatcg gcgttgtggt caacgctccg acgtcgaacg atgtgattgc
    13081 cgccgtccgg gaaaccattg atattccggt cgtggttacg gttgtttcca gccaggacga
    13141 tattggagcc cgtctggagg cgggagcatc catcattaat gtgtcgggag gcgccgccac
    13201 gccggaaatc gtagcggaga tccggagtcg ttttcccgtt cttccgatta tcgctaccgg
    13261 cgggcctgac gaaaaaacga tcagcaggac aattcgtgcg ggggcgaacg ctattacatg
    13321 gactcctccg agcgggtcgg aaattctggg ccggatcatg gaaagctacc gggaagagga
    13381 accctcgggc ggataagccg gtttatcttc tcgtctcata tcaatttata atatggcgcc
    13441 tgatttgtga attaattttg tggaaagcca caattatgaa taaatattca ttaaatgctt
    13501 gatttaatga atatttatta gtataatgag ttcacaaacc aacggccgcc atattttttt
    13561 atcaagaaat atggatattc gggaatcgta aggccggtta ttatcggagg cgtaagggaa
    13621 tgaaagaaat aaagaaagtt gttttagcat attcgggcgg gctggataca tctatcatca
    13681 tcccgtggct gaaagaaaat tacggctgcg aagtgatcgc cgtggccggc gacgtgggtc
    13741 agggaaagga gctttccggc ttaaaggaaa aggcgattaa gaccggtgct tcaaaaatat
    13801 atatcgagga cctgaccaaa gaatttattg aagattttat ctggccgaca ctcaaggccg
    13861 atgcaaaata tgaagattat ctgcttggaa cgtcgtttgc acgtccgatc atcgccaaac
    13921 gtcttgttga gattgccaag gcggagggtg cggacgccat agtccacggc tgtacgggca
    13981 agggaaacga tcaggtgcgc tttgagctga cgatcaaggc tttcgcccct gaaatggaaa
    14041 ttatagctcc ctggcgtatt tgggatctca aatcaaggga tgaggaaatc gactatgccg
    14101 aggcgcatca catcccgctc aaaatcacaa gggaaaccaa ctattccaag gatatgaatc
    14161 tctggcatct cagccacgaa gggctggatc ttgaagatcc cgcaaacgag ccgatgtatg
    14221 acaaaatact ggagcttggc gtttctcccg aaaaggcgcc ggatcaccct acttatgtta
    14281 ccatcgggtt tgaaaaaggc attccggttt ccatcgacgg cgtcaagacc ggcagcgttg
    14341 aaatgattca aaaactcaac aaactgggcg gagaaaacgg catcggtatt cttgacatag
    14401 tcgagaacag gctggtgggt atgaagtcga gaggaattta tgaaacgccg ggcggtgaga
    14461 ttctttatca cgctcacaaa acgctggaaa cgctgaccct tgataaagaa accttccatt
    14521 ttaaagagat catcggtcag aaattcgccg aagttgttta taacggtcag tggtataccc
    14581 cgctccggga agctctgtcg gcgttcaccg acaagaccca ggaaaatgtc acgggagaag
    14641 tcaagctgaa gctttacaag ggtaatatta tcaacgccgg aacgacctcg ccctactcgc
    14701 tctattccga agaagtcgca acctttggtg aagacgaggt ttacaaccag aaggattccg
    14761 aaggattcat taatttgttt ggtcttccga ttaaggttca ggccatgctg cagaagagcg
    14821 gcgcagagtc gaagtaattc caaatttgca aagataccgt tcgaggagtt ccatacactt
    14881 agactgtcgt atggaactca tcaattttaa accgttccgc cgccgtggaa agccgggtgg
    14941 ttcggctctt cagaataaag ggaggtcttt tccgtgaaaa tgtgggcggg acgtttttcc
    15001 aaagaaaccg acagccgggt gaatgatttt aattcctcca tctcgtttga ctgccggatg
    15061 ttccgtgagg atatcggcgg aagcatggcg catgccgaaa tgttggggaa atgcgggatt
    15121 attcaaaaag aagaatccat tgctatcatt gccggcctga aagaaatctt acaggatatt
    15181 gaaaacggca ggcttctgtt tgaccccgat gctgaggata tccacatgtt tgtggaggcg
    15241 gagctgacca gacgtatcgg cagcgcgggc aagaggcttc ataccgcccg aagccgaaac
    15301 gatcaggtgg ctcttgatct caggctttat ctcagagcgg aaataacgga gatcaaggcc
    15361 gcgctttcgg gcctgattca gacgcttgcg aagaaggcgg atgaaaattt aacggcaatc
    15421 atgcccggat atacgcactt gcagagggca cagccggtta cctttgccca tcatctgctg
    15481 gcgtatgcgc aaatgttcgc ccgggatctt acaaggcttt ccgactgcgc caaaagaatg
    15541 aattgttccc cgttgggatg cggggcgctc gccacaacga cctattccat cgaccggtcg
    15601 atgacggcag acctgcttgg attctccgct ccgacggcaa acagcctcga cggcgtatcc
    15661 gacagagatt ttgtcattga gcttgccggc gctctgtcaa ttgtgatgat gcatctttcc
    15721 cggttttccg aagaaattgt tctctggtgt tcctgggaat tcaaatttat cgagcttgac
    15781 gacgcttatg ccaccggatc aagcattatg ccccaaaaga agaatcctga tgtggcggaa
    15841 cttgtccggg gtaaaaccgg gagagtttac ggcgatctga tcgcgctgct gaccgtgatg
    15901 aagggtcttc ctctcgccta taacaaagat atgcaggagg ataaggaggc cgtctttgac
    15961 gcgattgatc aggtgaagct ctgcgtttca acatttgcgc cgatggttga gaccatgacg
    16021 gtattaaagg atcatatgag gcaggccgcg tccaaaggat ttatcaacgc gaccgactgc
    16081 gcgtattatc tgacgaaaaa gggtattccg ttccgggatg cttataagat taccggaagc
    16141 ctggttgctt actgtattga aaagggaaag acgctggaag tgctggaact tgccgagtac
    16201 cggaaatttt gttccgaatt tgacgaaggc gtctatcagg cgatttcgct tgaccgctgt
    16261 gtttcgggca gaagcgtaca gggcggcccg gcccccggag tcggtgaaac agcagctgaa
    16321 acaaatcaat gcgctgctgg cggagtacca aaaacaggat tctatcgggg aataatctca
    16381 atcagctttc tgaaaacgcg ggcgtttcga cgtcggttca cgggctgtgc gatttggcgg
    16441 gggatcatta tgaaatttaa aatctttgtc gacggacagg aaggcacaac cggcctgaaa
    16501 atttatgaaa gactggggtt gcaggaacaa attgatgttt tggcgatcac tcccgctctt
    16561 cgcaaggatc cgtcggaacg acgaaggctt ttgaatcagg cggatgttgc ttttctctgt
    16621 cttccggacg cggcatcgag agaagcggtt tcgatgatcg aaaacgaaaa tacgcgcgtg
    16681 attgattcga gtacggcatt ccgatgcgat ccgggctggg tttacggctt tcccgagctt
    16741 gaccggtcgc agagagataa aataatacac gcgaaacggg ttgccgtgcc gggctgccac
    16801 gccacaggat ttcattccat tgtccatccg ctggttacgg gagggctgat tgaaacttct
    16861 tatccgctga cctgccactc ggtttccggt tacagcggcg ggggaaaaaa gctgattgcc
    16921 cgatatgagc aggttcagac cggcgactcc ggtcatgaac agtcgctcag gggtctccgc
    16981 tattacgcgc tggggctgcg ccataagcat ctcgccgaga tgcggtatgt caacgggctt
    17041 gatcggcctc cgctttttac gccgtcggtg ggtgacttca gccggggaat gctggttacg
    17101 gttcccctgt ggaagaacca gatgaaaaaa ccgatgggtg cagccgaggt ccgggactat
    17161 tatgcagagt attatcaggg cgaaaagttt atccgcgtca tgccgtttga cagcgaacgt
    17221 ttccttgaag acgggtttct cgacgccatg ggatgcaacg gcaccaacag tctggaatta
    17281 ttcgtgtttg gtcatgatga ccagatgttt attgtctcgc ggcttgacaa tctgggaaaa
    17341 ggcgcatcgg gggcggccgt ccagtgtatg aacctgatgc tggggcaaag ggaagacgcg
    17401 ggactttttt aaaaaaataa gagaagatgg agtcaacagc tatgaaatat atagatggtt
    17461 ccgtgacggc gcctctgggc tttcaggctt ccgggatgca ttgcggaata aggaagaatc
    17521 gttccaaaaa agatctggcg ctgatttttt cgcagcaaga atgtgacgcg gctgcggttt
    17581 atacccaaaa caaagtcaag ggcgcgccga ttacggttac aaaacagcat ctcgcaaacg
    17641 gcaaggcaag agcagtcata tgcaacagcg gcaacgccaa tacctgcaac gccgacggcg
    17701 aggaaatcgc ggacttgatg tgcaaacata ccgcaagggt gcttggcatc agcgaggatg
    17761 atgtgattgt cgcctccacc ggcgtgatcg ggcagcccct tccggtggaa ccgattatca
    17821 gcagcctgcc gaagctggcc gggctgttgg cggacgacgg cgcggcacag gcggcggaag
    17881 ctatcatgac caccgatacc cgaaaaaaag aagtcgcggt ggaatttctc ctcaatggga
    17941 aaccctgccg gatcggcggc atcgccaaag gctcgggaat gattcacccc aacatggcaa
    18001 ccatgctctg ctttctgacg accgacgcgg atattgattc cggcctgctt gctcaggcgc
    18061 tgacggcaag cgttatgaaa tcctttaata tggtgagcat cgacggcgac acttccacga
    18121 acgatatggt ttccattctg gcgtccggcc tttgcggcag cggaagaatc gtttcggcgg
    18181 acgagagtta tcagacattc tgcggcgcgc ttgacgagat tacgatctcc cttgcaagag
    18241 aggttgcaag agacggagag ggcgcgacca aactgcttga atgcagagtg accggcgcga
    18301 agacggagca ggccgccaaa gcggttgcca agtcggttat cggctcgagt cttttcaagg
    18361 cggcaatgtt tggggcggac gccaactggg gccgcgtatt gtgtgcggtg ggttatgccg
    18421 acgccgattt ggatattgga aaggtcgatg tggcctttga atcggagaat ggtctgatcg
    18481 aggtttgcaa atgcggagcg gggcttgatt tttcagaaga aaaggcccgc tcggtactga
    18541 tccatgacga gatcgtcatc catatctcgt tgggggacgg agagtttgaa gcggcggcgt
    18601 ggggatgcga tcttacttac gactatgtga aaattaatgg agattacagg acctgatgag
    18661 agaaaggcct gtgacactgg aggataaaat gaaccaggtt tccgatcatt ataccgcgcg
    18721 ggttttagta cacgccctgc cttatattca ggaatataac aataaaaccg tggttgtcaa
    18781 atatggcgga aacgcaatga tcaaccggga actgaaggac gcggtgatga ccgatctggt
    18841 tctgctatcg ctggtgggaa tcaaaattgt tcttgttcac ggcggcggcc cggagatcac
    18901 cgaaatgctg gagaagatcg ggaaggcgag cctttttgtc ggcggtttaa ggtacacgga
    18961 tgctgagacg atggatatcg ttaaaatggt gctggcgggg aaggtcaaca aagaacttgt
    19021 gcagagtctt gaaatgaaag acggacacgc gctggggctt tgcgggctcg acggaggcat
    19081 gatccgggcg gaaaagctgg agcccggcgg agtggacctc ggctatgtcg gtaaaattgt
    19141 ttcagttgac gtaacaccaa tcaacgatgc cctttcaaaa ggatatatcc ccgtaattgc
    19201 tacgatggga gccggccggg acggcagcgt ttataacatc aacgccgaca cggcggcggc
    19261 ccggattgcc tccgatatcg gagcggagaa gctgatcctg ctgaccgata ccagagggct
    19321 gctcagagac agggaagacg aggaaagcct gatctctctt attcatgtca gcgacgttcc
    19381 cggccttgtc aatgagaata ttatttccgg cgggatgatt cccaaagtcg actgctgtgt
    19441 cgaggcggtt cgccggggcg tgaaacgggc gcatatcatc gacggccgga ttccccactc
    19501 gattcttgtg gaaatgttct ccgatgaagg aatcggcaca atgtttcaat aattaaaaaa
    19561 cataacggga ggtgtgccat ttgacggaat ttcaagaggt taagaatgca tccgatcaat
    19621 atctgatgcc cacctacacg cggtttaacg ttgccctgcg gtccggaaaa ggagcgaccg
    19681 ccgtcggcgt tgacgggaaa aaatatctgg atttcggaag cggcatcggc gtcaacgcgc
    19741 tgggttacag cgacgaaggg tgggttcggg ccgtcagcgg gcaggccgct ctgctttccc
    19801 atacttccaa tctatattac agcccggttc aggccgagct tgccgggctg ctttgcagtc
    19861 tgaccggatt cggcgcggcg tttctggcca attccggagc ggaggccaat gaaggcatga
    19921 tcaagctggc gcgcaaatac agttttgacc gctacggcga cggacgctca gtagttgtga
    19981 cgctgaaaaa ttcctttcac ggccggacgg tgacaacact ttccgccact gggcaggaag
    20041 aatttcaccg ctatttcttt ccgtttacct gcggctttcg ttttgccgag ccgaatgatt
    20101 tcgggtcggt caaagaagcc tgtaagggag acgtctgcgg gattcttctc gaggcggttc
    20161 agggggaggg cggcgtttat ccgctcgacc cggatttcgt taggcagacc gcagagttcg
    20221 cgaaagaaaa cgacattctg ctgatgtttg acgaggttca gaccggggtg gggagaaccg
    20281 ggcggctttt tgcctatgag cattacgggg tgtcgcccga cgcaatatca tccgcgaaag
    20341 cgcttggcgg cggtcttccg atgggggccg tgctatgctc cgaaaggctg aaagaggtca
    20401 tgggccccgg cagtcacggc tcgacattcg ggggaaatcc aattgcctgc gccggggcga
    20461 tcgaggtact cagccgggtt tccaaaccgg agtttctcat taaggtaaat gaaaaggccc
    20521 tgtattttcg aaaaaagctt gctgaaatca aagacgtggc ccgcatcagg ggtctgggac
    20581 tgatgatcgg ttttgaagtg gatggcgtcg attcaaagca ggccgccgcg aagctctcgg
    20641 aaaacggtct tctggtgctg acggccaaaa aggccctgag gctgctgccg ccgcttgtga
    20701 ttacaaagga cgagattgat cggggcatcg aaattttaaa aacggttttt gccgaactaa
    20761 aacatacgga gggatgagcg catgaaacat ctgttaaagc tgcttgattt aagcaaagaa
    20821 gaaattctgg atatccttaa tcttggcgat cagctcaaat atgagaaaaa acacggtatt
    20881 tcccacccga ggctcgcggg gaaaacgctg ggaatgattt ttgaaaaggc ttccaccaga
    20941 accagagtat cgtttgagac cggcatgtat cagcttggag gaaacgcgct gttcttaagc
    21001 tccaaagaat cacagatcgg caggggcgaa ccgatacagg acaccgcccg ggttttatcc
    21061 cgctatttaa acggaattat gatcagaacc tttggccaga aggaagtcga ggatcttgcg
    21121 gcgtatggat cgatcccgat tatcaacggc ctgaccgact ttgctcatcc ctgccaggtg
    21181 ttggccgatc tgatgaccat ccgggaattc aaaggaaagc ttgacggact taagctctgc
    21241 tttatcggcg atgggaacaa catggcgaat tccctgatcg tcggtgggct gaaagtgggt
    21301 atggaggttt cggtcgcatg ccccgaggga tatgatcccg accccgtggt tctggagttt
    21361 gcaaagacgt tcggcgacaa attcaggctc tgccgcgatc cggagaaagc cgcgggaggc
    21421 gcggatgccg ttttcaccga tgtctgggct tccatgggtc aggaaagcga gtcgcagaag
    21481 agaaaactga agttcgacgg ctatcaggtc aacgataaaa ttatagctgc cgccaacccg
    21541 ggaacgatgg tgcagcactg cctgccggcc cacaggggcg aggaaatcac cgaaaaggta
    21601 tttgaggcgc acgctgatga aatctttgag gaagcggaaa acaggctgca tgcgcaaaag
    21661 gccgtgctgg tgcggctgat ggagtaagat tcagggacgt tttgcccatt tgaggcggcg
    21721 tccctttttt ctgtttttcg gttgttgtaa aggcgggacg ggcgtgataa aataaatata
    21781 atttacaaaa aacggattct gagaaaccgg aattcgacat aggaatgaag gagttctatt
    21841 tcggcggaat ccattccgcc ccgaataggt ggtaggttat gaccggatgg ctgatgtttt
    21901 ggctggttgc tgtgattctg ctcgcgatta ttgaaattgc gacgacgcag cttgttgcaa
    21961 tctggatggc cgtcggagga gtgggcgcgc tggttgcctg ctcgttcaac gcctcggttc
    22021 tgacgcagtt cttaattttt atcgcaattt ccgcagcatt gctccttttg acaagaccga
    22081 tggtgaaaaa atttttgacg gtcaagcagg tgcggacgaa tgccgacagg ctgattggcg
    22141 gcattgcgac cgtgacggag gaaattgtca acgacgaaag caggggagag gccaaaatcg
    22201 gcggcgttac atggatggcc agatcgctgg acggtgagaa gatccccccc gaccaaaaag
    22261 ttcaggtgga gcgcattgaa ggcgcgaagc tgattgtatc caaattatag tgcgacggag
    22321 gaattgatta tgccttttac tactgttgtc tggcttgtgc tggctgttct tgttattatt
    22381 ctgctggtca gctgtttccg gatcgtgccc caggctcatg cttatgtcat tgagcgtctg
    22441 ggtgcctatc acaccacatg gggctccggc agtatcaaat ttaaagcccc ctttgtcgac
    22501 cggatcgcca aggtgatttc cctcaaggag caggttgtgg attttccgcc ccagccggtg
    22561 atcaccaagg ataacgttac catgcagatc gataccatcg tcttttttca ggtgaccgac
    22621 ccgaagctat acacttatgg tgtggagcgg ccgcttcagg ccattgaaaa tctgacggcc
    22681 acgacgcttc gtaatatcat cggtgatctt gagctggacc acacgctgac atccagagac
    22741 gtcatcaaca ccaaaatccg cacgctgctt gacgaggctt ccgacccctg gggcatcaag
    22801 gtcaaccgtg tggagcttaa aaatatcgtg ccgccccgcg aaatacagga cgcgatggag
    22861 aaacagatga aggcggaacg tgaacgccgc cagtccgtcc ttcgggcgga gggcgaaaag
    22921 accagccaga ttctggtgtc cgagggccag aaggaagcgc agatccttca ggctgaagcc
    22981 cagaaacagg cggcaatcct gcacgcggag ggcgttaagg aagcaaaaat aagggaagcc
    23041 gagggcgagg cggccgcgat tctcaaagtt caggaggctg tcgccgaagg aatcaagctg
    23101 ctcaatgttt catcgccgac cgatcaggtt atcgcgctga aatctctgga tacgcttgcc
    23161 aaagtggcgg atgggaaagc aaccaaaatt atcattcctt cagagcttca gtcgatcgca
    23221 tcactggcgg tttcggtgaa agaactggtg accggtgata aagccgaaca gcaggaaacg
    23281 caaaatagtt aaaacctcaa gctttttttc gaataaaaaa gtgcagttta gacaaattag
    23341 tcggctgagt tgtgaaaagg cttgaaatag tcggccgttt acaataaaat ataaatagga
    23401 attcagtcac agacaaagta ccttgacgtt taattttgcg gaggtgtaca atttgtccgt
    23461 aatggtgaaa cggagttgct ccatgctgtc atcaggatca gacggccttg aaaatagtta
    23521 tgttttgaat acttctgcga tggggcatac aatataagtg caagcaaaat acacttctca
    23581 cgaagtgtat ttttttcttt tcatgtaatt atcaggaaaa atcaggtgtg ggaggccatg
    23641 aaatgagcga aagtaaaaaa gtagccgtaa ttatgggcag tgacagtgac ctgcctgttt
    23701 taaagggctg tatcagaacg ctgaaagagc tgggaattcc ggccgaggtc catgtaatgt
    23761 ccgctcacag aacgccggac agagcggcgg aattttctaa gaatgccatt cggaacggtt
    23821 ttggtgtcat tatcgcggct gcgggcaagg cggctcatct cgccggcgtt ctcgcggctg
    23881 gaacgactct tccggtgatc ggggttccgg tcaaatcttc aacgcttgac gggcttgacg
    23941 cgctgctttc caccgtccag atgcccaagg gtatcccggt cgccacagtg gccatcgacg
    24001 gcgctgacaa tgcggcaatc cttgcggcgc agattttggc ggtatccgat caggggcttg
    24061 ccgaaaagct tgcggaaatg aagaagaata tggctgacgg tgtgatgaaa aaagatcaaa
    24121 acatacaggt ggaggtagaa aaaatatgaa aaaacttgaa caggtatatg agggcaaagc
    24181 gaaaaaggtt tttaaaaccg atgacccgaa tttgtttatc gtcgactata aggatgacgc
    24241 gactgcattt aacggcctga aaaagggaac gattcaaaat aagggtatca tcaacaaccg
    24301 tgtaaccaat tatctgatga aaatgcttga gagcaaagga attcacaccc attttgtc
//
LOCUS       DATALY010000369         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_79459,
            whole genome shotgun sequence.
ACCESSION   DATALY010000369 DATALY010000000
VERSION     DATALY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79459"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VHR42_10115"
     CDS             complement(<1..1039)
                     /locus_tag="VHR42_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3027559.1"
                     /translation="MDNYIGKKLDGRYEVQEIIGVGGMAVVYKAYDCIDDRTVAVKIL
                     KDEYLTNEEFRRRFKNESKAVAVLSHPNIVKVYDVSFGEKFQYIVMEFIDGITLKEYI
                     DQQKNLTWKEALHFTVQILRALQHAHDRGIVHRDIKPQNIMMLEDGTIKVTDFGIARF
                     AHSETRTMTDKAIGSVHYISPEQARGGYTDEKSDIYSVGVMLFEMLTGELPFEADSAV
                     SVAIMQLQQNPKQPREINPDIPEGLEEITLRAMQKDPAQRYASASAMLDDIEEFKRNP
                     SAHFEYKYFVDESPTKYVDAINKIKKTEEPEGKKQKAKKAHAIPILSGIAAALLLIAL
                     IVVFIALYANGY"
     gene            complement(1045..>1762)
                     /locus_tag="VHR42_10120"
     CDS             complement(1045..>1762)
                     /locus_tag="VHR42_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX3027560.1"
                     /translation="KLRKTNQDDFACGMFPDGGVWAAVCDGMGGASGGSVASSVAVKV
                     ISEKITGGYKGQDSDGIKDLLFESIAAANTAVFQKAQEVPELSGMGTTVVAGIAAPST
                     GKLHIVHAGDSRAYLIASDSIRQITRDHSIVQDMLDTGKLTPEEAKTHPQKNIITRAL
                     GVEENLDIDYSEADFNEDGTVLICTDGLTNHVDTDAIAEVVNNTDYEQCPARLIDLAN
                     QSGGSDNITVVVMNNCLNRE"
BASE COUNT          369 a          491 c          415 g          487 t
ORIGIN      
        1 aataaccgtt ggcataaagc gctataaaca caacaatcag cgcaatcagg agcagcgccg
       61 ccgcgattcc ggaaagaatc gggatggcat gggctttttt cgctttttgt ttcttgccct
      121 ccggttcctc ggtcttttta atcttattga ttgcgtcgac atatttggtg gggctttcgt
      181 cgacaaaata tttatactcg aaatgggcgc tggggttgcg cttgaattcc tcaatatcat
      241 cgagcattgc cgaagcggac gcataacgct gggccgggtc tttctgcata gcgcgcaggg
      301 taatctcctc aagaccctcg ggaatgtcgg ggttaatctc cctcggctgc ttcgggttct
      361 gctgcagctg catgatggcg accgaaacgg cgctgtccgc ttcaaaggga agctctcctg
      421 tcagcatctc aaacaacatg acgcccaccg aataaatatc cgatttttcg tcggtgtagc
      481 cgcctcttgc ctgctcgggg ctgatatagt gaaccgaacc gattgccttg tcggtcatgg
      541 ttctcgtttc ggaatgggca aaacgcgcga ttccgaaatc ggttactttt atggtcccgt
      601 cttcaagcat cataatgttc tgcggcttga tatcgcggtg aacgatccca cggtcgtgcg
      661 cgtgctggag ggcgcgaaga atctgaacgg taaaatgcag cgcttccttc caggtcaggt
      721 ttttctgctg gtcaatgtat tcttttaacg tgatgccgtc aataaattcc atgacgatat
      781 actgaaattt ttccccgaaa ctcacgtcat acactttgac aatattcggg tgtgacagca
      841 ccgctaccgc tttggattca tttttgaagc gccgccgaaa ttcctcgttg gtcagatatt
      901 catcttttaa aattttaaca gccacagtcc tgtcgtctat gcagtcgtag gccttgtaaa
      961 ccacggccat tcccccgacg ccgatgattt cctgcacttc gtatcttccg tctaattttt
     1021 taccaatata attatccatc tgaatcactc ccggttaagg caattgttca taaccactac
     1081 ggttatatta tcgctgccgc cgctttggtt ggcaaggtcg atcagccttg ccgggcattg
     1141 ttcataatcc gtgttgttca ccacttccgc gatcgcgtcg gtgtcaacat gattcgtcag
     1201 cccgtcggtg cagatcagca ccgtcccgtc ctcattgaaa tccgcctcgc tataatctat
     1261 gtcaaggttt tcctcaaccc cgagggcccg cgtaatgata tttttctgcg ggtgcgtctt
     1321 ggcttcttcc ggggtgagtt tcccggtatc gagcatatcc tgaacgatgg aatgatcccg
     1381 cgttatctgc cggatactat cgctcgcaat cagatacgcc cggctgtcac cggcgtggac
     1441 aatatgcagt ttccccgtcg aaggcgcggc aatccccgcg accacggtgg tgcccattcc
     1501 ggaaagctcg ggcacttcct gagctttttg aaaaacggcc gtgttggccg cggcaatgga
     1561 ttcaaacaaa aggtctttga ttccgtcgct atcctgccct ttatagccgc ccgtaatttt
     1621 ctctgaaatg actttcaccg ccaccgaact cgcgacgctg ccgccgcttg ctcctcccat
     1681 gccatcacag acggccgccc atacgcctcc gtccggaaac atgccgcagg caaaatcatc
     1741 ctgattggtc tttcttaatt tc
//
LOCUS       DATALY010000370         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_594461,
            whole genome shotgun sequence.
ACCESSION   DATALY010000370 DATALY010000000
VERSION     DATALY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594461"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VHR42_10125"
     CDS             <1..925
                     /locus_tag="VHR42_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX3027561.1"
                     /translation="EKVDRVPSAFWLHFRKEQFHGENAVKAHLDLFEKTDVDILKVMN
                     ENKLTCSCPFGKNIDNWKHIRPLSLKDAFVADQLEIIKRVVDSVGDQAVVIATIHGIV
                     ASASHMSGEHYADFRKILPAQLKEKPGIVADGLRIIAEGLAEFSRACLEAGAQGIYYA
                     ALGGESYLIGDEDFDRYIKPNDLMVIEASKSPDAFNSLHICKDHVSLKRYADYHPQVV
                     NWGVYENNPTLLEGKKYFPDAVLLGGLDDNPGSAIVSGTDKEIEEEVQSLLNQIGTRK
                     FILGADCTLPTDINYERIRTAVEATAKYTAE"
     gene            935..1600
                     /locus_tag="VHR42_10130"
     CDS             935..1600
                     /locus_tag="VHR42_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3027562.1"
                     /translation="MGINPMEHKKTLKEEIRNQIIDRVVAGEFNSDSILTEKMLTSLF
                     AVSKAPVREALIELCNEKILQSIPRCGYRIVRLTPRDIAQAVEVRSVLEFAAFDKMAA
                     CLSPEMTEPLKCFNVECDRSRISADVWTHWNNNREFHLLLAGLCGNLLLIEMLGKTLG
                     LLSRAYAQSYWEEWHDSLVSLDIEKHKLIVAALEQGKIKTARSALQTDLQLMAKRFHL
                     PGA"
     gene            complement(1680..2222)
                     /locus_tag="VHR42_10135"
     CDS             complement(1680..2222)
                     /locus_tag="VHR42_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX3027563.1"
                     /translation="MSEVLKSGMPAPDFTIQTPWGKTETFYKMADGKKCFLFFLRYVG
                     CTLCQVEIHQLIQSYHRFAEKDAKFFVVLQSAPENIRENLTQEQIPFTIICDPEQAIY
                     RTYHVKAAENQSEMGTPDLFADKLKIAQKNGFSHGKYEGNELQLPATFLIDQDHSILF
                     AHYGSHVGDTPDHGELLKLL"
     gene            2400..3572
                     /locus_tag="VHR42_10140"
     CDS             2400..3572
                     /locus_tag="VHR42_10140"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3027564.1"
                     /translation="MDSYFSKINQSRSTDEKLILVNLALMFCSCYISPFAVGLSAIYL
                     VLSRKRRQRFTLVPNLFWLGVWAGLTTVTGLMYRNLLGVACGLIFFLFILFGMYAWTI
                     MTDKLAGQIIRLAAIMSIAAFAIAVVQKLMGIDRTPATFFNPNFYAFACEVVFILCVS
                     ALFSRKAEPLLYSLALAANLGGILLSGCRSAWLAVLGGVLVFLICTGKYKACGISLSF
                     AAVCGIVLLLKPDLIPRSDDFGTTVTLRQMIWSAGYQVFLKHPLFGEGFLSYRFATVG
                     MGRAHMSHGSVIAHAHNILLDSLINFGLVGTAIALLFLVPAVLKWVKRRRHNPAGAIA
                     VAVFTAMLIHGITDVPVLGYQTGSLTMLLLALSGAVKSSGDDRRVWLPKFMRRKIG"
     gene            complement(3720..4355)
                     /locus_tag="VHR42_10145"
     CDS             complement(3720..4355)
                     /locus_tag="VHR42_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX3027565.1"
                     /translation="MIGIFFKSLLIGYSGAIMPGSMLTYTIDKSFHHGAKSGVLVALG
                     HSLVDMFLVILLFAGTGRFLTGDLTKSIIGLVGGIVLFYLGIKMAADAFLNKISLENQ
                     DSKSSGQRNMFFAGFILSITNPLVIVWWSVIGLALIMSSYSAFGIAGVAVFYFGHILA
                     DVSWLTFISALVGKTSRLLTPKVYKMIILLLAVCLIGFGINFLIGSIRVLF"
BASE COUNT         1179 a          985 c         1095 g         1172 t
ORIGIN      
        1 tgaaaaggtt gatcgggtcc caagcgcttt ctggcttcat ttcagaaagg aacagtttca
       61 cggcgaaaac gcggtgaagg cgcatcttga tctgtttgaa aaaacggatg tggatattct
      121 caaagtaatg aatgaaaaca aattgacctg ttcttgcccg tttggaaaaa acattgataa
      181 ttggaaacat atccgtccgc tgagtctgaa agatgctttt gtggcggatc agctggaaat
      241 tatcaaacgt gttgttgact cggtaggaga tcaggctgtt gtaattgcta caatccacgg
      301 catcgtagcc tccgcttccc atatgagcgg agagcattac gcggacttta gaaaaatcct
      361 gccggcgcag ctcaaagaaa agcccgggat tgtagcggat ggtttaagaa ttatcgccga
      421 gggtctggcg gagttttcaa gagcgtgtct ggaagcgggc gctcagggaa tttattacgc
      481 agcgcttggc ggcgaatcct atttaattgg cgacgaggat tttgaccgtt acataaagcc
      541 caatgatctg atggtgattg aagcatcaaa aagcccggac gcgtttaatt ccctgcatat
      601 ctgtaaagat cacgtcagtc tgaaacgata tgcggattac cacccgcagg tagtcaactg
      661 gggagtgtac gaaaataatc caactttact ggaaggaaaa aaatactttc ctgatgctgt
      721 cctgctcggc ggattggacg ataatcccgg cagcgcaatt gtttccggaa ccgataagga
      781 aatcgaggaa gaggtgcagt ccctgctcaa tcaaattggt acgcgcaaat ttatccttgg
      841 ggcggactgc acactgccga ccgatataaa ttatgagaga attcgcacgg cggtggaagc
      901 cacggcgaaa tacacggcgg agtagagtct gattatggga atcaatccga tggaacataa
      961 aaaaacgctg aaagaagaaa tccgaaatca aatcattgac cgggtcgtag ccggagaatt
     1021 taattccgac agcattctga cggaaaaaat gctgaccagt ctgttcgcgg tcagcaaagc
     1081 tccggttcgg gaagcgctga ttgagctctg caacgaaaaa atactgcaaa gtatcccacg
     1141 gtgcggatac cggatcgtca ggctgactcc gagggatatc gcacaggcgg tagaagttcg
     1201 gtcggttctg gaatttgcgg cgtttgacaa gatggctgcc tgcctgagcc cggaaatgac
     1261 cgagcctctt aaatgcttta acgtggaatg cgaccggtca agaatttcgg cggatgtttg
     1321 gactcattgg aataacaaca gggagtttca tctgttgctc gcaggtcttt gcggaaacct
     1381 tctgctaatc gagatgttgg ggaaaacgct ggggctgtta tcccgtgctt atgcccagag
     1441 ctattgggag gaatggcatg acagcttggt gagtctggat attgaaaagc ataaactgat
     1501 cgtggcggct ctggagcaag gaaaaatcaa gacggcccgg tccgctttgc agacggattt
     1561 gcagttaatg gccaagcgat ttcatttgcc gggtgcataa aaattccgcc ggcgcacagg
     1621 aaaaggcctc ccacggtttt gggtttgata ccgaaaccgt gggaggcctt gttctccaat
     1681 cagagtaatt tcaaaagctc accgtggtcg ggcgtgtcac cgacatggct gccgtaatgg
     1741 gcgaaaagaa tagaatggtc ttgatcaatc aggaaggttg caggaagctg aagctcgttt
     1801 ccctcatact tcccgtgaga aaagccgttc ttttgggcga ttttaagttt atccgcaaac
     1861 aggtcggggg ttcccatttc cgattggttt tcggcggctt tgacatgata agtacgataa
     1921 atcgcctgtt ccgggtcaca aataatggta aatggaatct gttcctgtgt gagattttca
     1981 cgaatgtttt ccggcgcact ttgtaaaacg acaaaaaact tagcgtcttt ttcggcaaac
     2041 cggtgataac tttggatgag ctggtgaatt tcaacctgac acagcgtgca gccgacatac
     2101 ctcagaaaaa aaagaaagca tttcttccca tcggccattt tgtagaaagt ctccgttttg
     2161 ccccagggcg tctgaatggt aaaatcggga gcgggcatgc cacttttaag aacttcagac
     2221 atgataattc ctcctaatat tatcctccgg tggttttttc aaccgactga cgaaggtttt
     2281 ttagcaaata gaacggcttt gaccattcat tgattgacac ttccaaaaca gataagatat
     2341 aattgtttca tataaccgtt ctgcttaacc catttaaaaa gactggaaag aagattggaa
     2401 tggattctta tttcagtaaa attaatcaaa gccgctccac agacgagaag ctgattcttg
     2461 tgaatcttgc cttaatgttt tgctcctgct atatcagccc gtttgcagta gggctgagcg
     2521 ctatttatct ggtactcagc cgaaaaagaa gacagcggtt tactcttgtt cccaatctct
     2581 tttggttggg ggtatgggct ggtttgacaa ccgttaccgg tcttatgtac cgaaatttat
     2641 tgggggttgc ctgcggcctt atcttttttt tgtttatctt atttggaatg tatgcctgga
     2701 caataatgac ggacaagctt gccgggcaaa tcatccggct cgcggccata atgagtatag
     2761 ccgcttttgc catagctgtc gttcagaaat taatgggaat agacagaacg ccggcaacct
     2821 ttttcaaccc caatttctac gcatttgcct gtgaggttgt atttatactg tgtgtttccg
     2881 cccttttttc aagaaaagcc gaaccgttgc tctattctct cgccttagcg gccaatttgg
     2941 gcggcatatt gctctccggc tgtcggtcgg cctggcttgc cgtattgggc ggtgttctgg
     3001 tgtttctgat ctgcactggt aaatacaagg cttgcggtat cagtttatct tttgccgcgg
     3061 tctgcggtat cgtactcctt ctcaaaccgg atttgatccc gcgttcagat gatttcggca
     3121 caacggtcac cctaaggcag atgatctgga gcgcaggata tcaggttttt ttaaagcatc
     3181 cgctttttgg agaaggtttt ctctcttatc ggtttgctac ggtgggtatg ggcagggcgc
     3241 atatgtcaca tggctccgtt atcgcgcacg cccataatat tttgctggat tcgctgatta
     3301 atttcgggtt ggtcggaacc gccatcgcgc tgctgttttt ggttccggcc gtcttaaagt
     3361 gggtgaaacg acgccggcat aatcccgccg gcgcaatcgc cgtagcggtt ttcactgcaa
     3421 tgttgattca cggaataacg gacgttccgg ttttgggtta tcagacagga agcctgacca
     3481 tgctgttgct cgcgctctca ggcgctgtaa aaagttccgg tgatgaccgg cgcgtctggc
     3541 tgccgaaatt catgcgccgt aaaataggat aagcttacag gccgatcccg gccaaagttt
     3601 ctgcgtttcc gccaaatgtc tcagcgacat tattgttagt atattccaat atttgataca
     3661 aatcaagcgg aaagtaagcg cgatttacat cgccatgatc cgccaccatt gctttcaact
     3721 cagaagagaa ctcgaatact gccgatcaaa aagttgatgc cgaaaccgat cagacagacc
     3781 gctaaaagca ggataatcat cttatatact tttggggtca ataaacgact ggtttttcct
     3841 accagcgctg agataaaagt taaccatgaa acatccgcta aaatatggcc gaaataaaac
     3901 acagcgacac cggcaatacc gaaagcggaa taagaactca taattagcgc aaggcctatt
     3961 accgaccacc agacgataac caatgggttt gttatactta aaatgaagcc ggcaaaaaac
     4021 atgtttctct gcccgctgct tttggaatcc tgattttcca gcgagatttt atttaaaaat
     4081 gcgtcggctg ccattttaat tcccaaatag aagagcacta ttccgccgac aagaccgatg
     4141 attgattttg tgagatcacc ggtcagaaac ctacccgttc cggcaaataa caaaatgacc
     4201 agaaacatat cgacaagact atgtcccagg gcgacaagca cccccgattt tgctccgtga
     4261 tgaaaacttt tgtcgatcgt ataggtcagc attgagccgg gcataatggc tcccgaatag
     4321 ccgatcagta atgatttgaa aaagatgcct atcataaagt acccccagac cagatttgac
     4381 aagggatgaa cgttttgtca aatccattct aaaccctcca gttactggag g
//
LOCUS       DATALY010000371         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_79474,
            whole genome shotgun sequence.
ACCESSION   DATALY010000371 DATALY010000000
VERSION     DATALY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79474"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..674
                     /gene="prfB"
                     /locus_tag="VHR42_10150"
                     /pseudo
     CDS             1..674
                     /gene="prfB"
                     /locus_tag="VHR42_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071422814.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(726..1724)
                     /locus_tag="VHR42_10155"
     CDS             complement(726..1724)
                     /locus_tag="VHR42_10155"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3027566.1"
                     /translation="MPYSREIFEKSIARLEERRAGAENEQQRLKKILYEKIPRLGQIE
                     KELAQTGIRSAKEILSGTGNAGVKIEQLKKRNLELQAERAEILTVCGCQPDILKISYF
                     CSKCNDTGFIGDRICDCLKTLLREESCKKANAGSPLPLCDFDSFSLAYYPENKQIESG
                     ISIRQHMSKVFSFCKKYASSFDRNSGSMIFLGQTGLGKTHLALSIANSVISSGFTVIY
                     DTSQNIFLKMEEEYFGRAPKKFSFSAMECDLLIIDDLGSEFNSSFNVSTFYNIINSRI
                     LSGRPVIVSANITEEELLSRYSERIFSRLIGDFILLKFFGSDIRQLKLQQKAGLAI"
     gene            complement(1748..>2130)
                     /locus_tag="VHR42_10160"
     CDS             complement(1748..>2130)
                     /locus_tag="VHR42_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEX3027567.1"
                     /translation="DTHEMAESKILQLEAHRSFEGKVKAVMGITSRNLSQTEKQHLNR
                     WHTEWAMSPELVKMAYDICVDRTGKLSFSYINSILKSWHEKGYTTIEQAGSERKQSAK
                     PTAKPHSFSVDEYVRLSMKRLHDE"
BASE COUNT          519 a          508 c          542 g          561 t
ORIGIN      
        1 accggatgta gacccactgg gctgagcgtc ataattttaa agtcaaggtt cttgattatc
       61 tcgatggtga ggaagcgggc ttgaaaagcg cttcggttct ggtggagggg cttaacgcct
      121 acggctatct caagtcggaa atcggcgttc accgccttgt gcgcgtttca ccatttgacg
      181 cttccggcag gaggcatacg tcttttgcct cgcttgaagt tatgccggaa atagacgaca
      241 cgatcgcggt tgacatccgt accgaagata tcaaagttga cacctatcgc agcggcggcg
      301 cgggcggcca gcatatcaac aagaccgaat ccgccatccg catcacccat ctgcccaccg
      361 ggatcgtggt aacctgccag aacgagcgca gtcagcatca gaacaaagag gttgcgatga
      421 gaatgctgaa atccaaactg gtggagatta aagaacgcga acatctcgac aagattgagg
      481 atatcaaggg tgtgcagaag gaaatcgggt ggggttctca gatcagatcc tatgtcttca
      541 tgccttacac gttggtgaag gatcaccgga ccaattttga aatgggaaat attaatgcgg
      601 tgatggacgg agatatcgac ggattcatca atgcttacct taaagcgatg agtcttgggc
      661 agatcgaaaa ataaaaaatg gtttcctgtt gcaagaacct ccgctgcgaa cagcggaggt
      721 tctttttaaa ttgccaatcc ggctttctgc tgcagtttca gctgccggat atcgctgcca
      781 aaaaatttaa gaaggataaa atcgccgatc agccttgaaa aaatccgttc gctgtaacgg
      841 ctgagcagtt cctcttccgt aatgttggcg ctgacaatga ccggcctgcc cgataagatt
      901 ctggaattga taatattata aaaagtagaa acattaaagg aagaattgaa ttcactgccg
      961 agatcatcga tgatcagcag atcgcactcc atagccgaaa acgagaattt tttcggagct
     1021 cttccaaaat actcttcctc cattttcaga aaaatattct gagaggtatc gtaaatcacc
     1081 gtaaagccgc tgctgatcac actgttggca atcgagaggg caagatgggt tttgccaagc
     1141 ccggtctggc ccagaaaaat catgctgccg ctgttccggt caaaggatga agcgtatttt
     1201 ttgcaaaagg agaacacctt cgacatgtgc tgcctgatgc tgattccgga ttcgatctgt
     1261 ttgttctccg ggtaataggc caggctgaac gaatcaaaat cgcagagagg cagaggggag
     1321 cccgcatttg cttttttgca ggattcttcc cgcagcagcg ttttgaggca atcgcagatc
     1381 cgatccccga taaaacccgt gtcattgcac ttggaacaaa agtagctgat tttcagaatg
     1441 tcgggctgac agccgcagac agtcagaatt tcggctcgtt cagcctgcag ctcgagattg
     1501 cgctttttca gctgctcaat tttaaccccg gcgtttccgg tcccggaaag gatctctttt
     1561 gccgagcgaa taccggtctg tgccagttct ttttcaattt ggcccagacg cgggattttc
     1621 tcgtaaagaa tttttttcag tctttgctgt tcgttttccg cgccggcccg tctttcttcc
     1681 agccttgcta tggatttttc aaagatttcc ctgctgtacg gcatgctccg tcctcctgtt
     1741 catcggttta ttcgtcgtgt agtcttttca tggaaagccg cacatactcg tccacgctaa
     1801 aggaatgggg cttcgccgtc ggcttggcgg actgcttacg ttcactgccg gcctgctcga
     1861 tggtcgtgta acccttctcg tgccaggatt ttaaaatgga gtttatgtag gaaaaagaga
     1921 gtttcccggt gcggtcgaca cagatgtcat aggccatttt aacgagttcc ggcgacatgg
     1981 cccactcggt gtgccaccgg ttgagatgct gcttttcggt ttgggagaga tttcggctgg
     2041 tgatgcccat aacggctttt acctttcctt caaaggaacg gtgcgcttcc agctgaagaa
     2101 ttttactttc ggccatctca tgggtgtcga
//
LOCUS       DATALY010000372         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_594939,
            whole genome shotgun sequence.
ACCESSION   DATALY010000372 DATALY010000000
VERSION     DATALY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594939"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHR42_10165"
     CDS             <1..190
                     /locus_tag="VHR42_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX3027568.1"
                     /translation="SIEVHVADIRTCVLHPASSTHRQLTEEQLVAAAINPGMIRFSVG
                     LESAEDIIADIDQSLSRV"
     gene            complement(488..1084)
                     /locus_tag="VHR42_10170"
     CDS             complement(488..1084)
                     /locus_tag="VHR42_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3027569.1"
                     /translation="MAELLYQGHDSYRITAQNGMVIYIDPFEGKGYDQPADLILVTHE
                     HTDHNKVSLVPQKQGCTTIRSSDALKSGEYRSFIIGGVKIEAVEAYNRNHKKEECVGY
                     ILTIDQVSVYAAGDTSKTEQMKKLGSRKLSWALLPIDGVYNMGPEEASECAALIGAAH
                     NIPVHMKPGKLFDRGMAESFHAPGRVILAPGESVELKK"
     gene            complement(1148..2083)
                     /locus_tag="VHR42_10175"
     CDS             complement(1148..2083)
                     /locus_tag="VHR42_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027570.1"
                     /translation="MENDRLKEIRAGLSALPVMEQCMNNLRRQLSDAQSEYERLKRKF
                     EAESQDVDKLYKESFTVMLLRKIGRYEGKLNKETQEQLAAKMEFDKAGDRVERLRSEE
                     ADAESRVEQLKNDRRQFEEELKRRRDEMKNNMALENHERFHKMEKQLQELSKQSAETD
                     QAISAAEKVLSTAQSALDSLQSAESWATYDVWSNGGILSHSAKYEAVDDAEIAMESVN
                     EQLENLHKELSDINLQDSIQFTPIDSTTRTIDFWFDNIFTDLNVRSRIQDNMDTLEVL
                     CKNLNDLMDSLKSSKSKIETKETELEQDEENLLVG"
     gene            complement(2116..3405)
                     /locus_tag="VHR42_10180"
     CDS             complement(2116..3405)
                     /locus_tag="VHR42_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEX3027571.1"
                     /translation="MNLVDGKIISSGECDAVLDELDQRILRTFQKGRLDPETVISACD
                     RLVSNLDEAQYLKKMAGIGIDEALGKSYLDEARRMFSEDSLRYRLSVELGEDYSRPNT
                     FTPSFRNYAVKERTFPLGVLLHIAAGNADGLPAFSVLEGLLTGNINILKLPAAEGGIS
                     VQILLELIKAEPSLAEYIYVFDYSSKDIVHIKKLISVTDAVVVWGGKEAVSALRCLVP
                     PNVRLMEWGHKASFAYITEQGITESGLLGLAKNITQTGQLLCSSLQGFFIDTDNMNLV
                     CDFCGRFLPVLEDAVRKSPQKIGIGIRSQVALKLYNEELDELYRNSKVFRGVNCSLTA
                     YPDTILETSIPFGNPWVRPLPRSKLLSALRPYKNYLQTVGLLCSAAELEDLSEILFQA
                     GAVRVCRGENMSDTYCGAPHDGEYSLRRYTKIVSLEN"
     gene            complement(3402..>3981)
                     /locus_tag="VHR42_10185"
     CDS             complement(3402..>3981)
                     /locus_tag="VHR42_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HEX3027572.1"
                     /translation="LIHYEKSGFPVRFMGFPAYFMFLIKELMNSRIHLKLHPKSLVIL
                     AGGWKQFFAERVDKQTLYQMSKETLGIGGDRIREFFGAVEHPIAYFDCPNHHFHVPVY
                     SRVIIRDTELNPVKYGEPGLLNLITPMMTSMPFVNVMTDDLAVLHPGEECGCGIHSPY
                     FDILGRVGLADIKTCAAGASELLNVLQKGGTK"
BASE COUNT          983 a         1063 c          838 g         1097 t
ORIGIN      
        1 atcgatcgaa gttcatgttg ccgatatccg cacctgcgta cttcacccgg ccagctcaac
       61 gcaccgccag ctcacggagg aacagcttgt agccgcagcc ataaaccccg gcatgattcg
      121 cttctcggtc ggtcttgaaa gtgcagagga tatcatcgcc gatatagatc aaagcctatc
      181 gagggtataa ttagtacaat actattttct ttgaaacagc caaaaatttt ttattaacta
      241 ggcctaaact caattttcaa attctctcta ttaaaaataa atataaagca gcaacggcat
      301 atccggcctt cgcatctggc cgggtatgcc gttttataga atctaaagca aaactgaatc
      361 agccgctctt tcttttcgct attctttaac gtaaaagtaa aacgatttcg taccccaggg
      421 ctgaactgta atagtttttg cgagctgagc ataaacaggt gtttttaaat ttcacatcac
      481 ccaaccttta ttttttcagc tcaaccgact ccccgggtgc aaggatgaca cggccgggcg
      541 cgtgaaagga ttccgccatt ccccggtcaa aaagttttcc cggcttcatg tgcaccggaa
      601 tattgtgagc cgctccgatc aatgccgcgc attctgacgc ttcctcaggc cccatgttat
      661 aaacgccgtc gatcggcaga agagcccaac tgagcttccg gctcccgagt tttttcatct
      721 gttccgtctt tgaagtgtcg cccgcggcat aaacggacac ctgatcaatc gtcaggatat
      781 acccgacgca ttcctccttt ttgtggttgc ggttataggc ttcgaccgct tcaattttga
      841 cgccgccgat tatgaaagaa cggtattctc cgcttttcag cgcatccgat gatcgtatgg
      901 tggtacatcc ctgcttttga gggaccagag aaaccttatt atggtccgta tgttcatgcg
      961 ttaccaatat caaatccgcc ggctggtcat aaccctttcc ctcgaacgga tcaatataga
     1021 ttaccattcc attttgcgcc gtaatccgat aactgtcgtg accctgatat aaaagttcag
     1081 ccatttccat cagcctctcg tgaaaatctt gcgttttttt ccgtcagcga cattaagccc
     1141 ataaacatca gccaaccagt aggttctctt cgtcctgttc cagctcagtt tccttcgttt
     1201 caatctttga tttgctgctt tttagggaat ccatgagatc attcagattc ttacatagta
     1261 cttccagagt gtccatattg tcctgtattc tggaacggac gttcagatcg gtgaaaatat
     1321 tgtcaaacca aaaatcaatg gtacgcgttg tggaatcgat gggagtgaat tgaatcgaat
     1381 cctgtaaatt gatatcggag agttctttgt gaagattttc aagctgctca tttacgcttt
     1441 ccattgcaat ttccgcgtcg tcgaccgctt cgtattttgc agaatggctc aggataccgc
     1501 catttgacca gacgtcgtag gttgcccagc tttctgcgct ttgcaggctg tccagcgcgc
     1561 tttgcgctgt tgaaagcact ttttccgcag cgctgattgc ctggtcggtt tcggcagact
     1621 gtttggaaag ctcctgcagc tgcttttcca tcttgtgaaa ccgctcatga ttttcgagcg
     1681 ccatattgtt tttcatttca tcccggcgcc gctttaattc ttcctcaaac tgccggcggt
     1741 cgtttttcag ctgctcaacg cggctttccg cgtccgcttc ctcagagcga aggcgttcga
     1801 cacgatcgcc cgccttgtca aattccatct tcgcggcaag ctgttcctgt gtttccttat
     1861 tgagcttccc ctcataccgc ccgatttttc taaggagcat cactgtaaac gattccttat
     1921 agagcttatc cacatcctgc gattccgctt caaatttgcg tttcaggcgt tcgtattcac
     1981 tctgcgcgtc gctgagctgc cgccttaagt tattcataca ctgttccata acgggcaggg
     2041 ccgagagacc cgcgcgaatt tcctttaatc tgtcgttttc catactgccg cctgctttcc
     2101 tatattcaaa tccagtcaat tttctaatga tacaatcttg gtatagcgcc gtaacgaata
     2161 ttcgccgtca tgcggagcgc cgcaatatgt atccgacatg ttttctccac ggcatacgcg
     2221 cacagctcct gcctgaaaaa gtatttccga taggtcttcg agctccgctg cgctgcaaag
     2281 aagcccgaca gtctgcaaat aatttttata aggccgtagg gcagacaata atttagaacg
     2341 gggcagcggc ctgacccacg ggtttccgaa cggaatggac gtttccagaa tcgtatccgg
     2401 gtatgcagtc agactgcaat taacgccgcg aaacactttg ctgtttcggt acaattcatc
     2461 aagttcttca ttataaagct tcagtgctac ctgcgacctg atgccgattc cgattttctg
     2521 cgggcttttg cggactgcgt cttccaagac aggtaaaaaa cggccgcaaa aatcacaaac
     2581 caggttcata ttatccgtat cgataaaaaa cccttgcaga gagctgcaca aaagctggcc
     2641 ggtctgcgta atatttttcg caagccctaa aagcccgctt tccgtgatac cctgctcggt
     2701 tatataagca aagctggcct tatggcccca ttccatcagc cgcacgttcg gcgggacaag
     2761 acagcgcagg gcagaaactg cttccttccc gccccagacg actacggcgt ccgtcaccga
     2821 aatcagcttc tttatatgaa cgatatcttt tgacgaataa tcaaaaacat aaatgtattc
     2881 ggcaagcgaa ggctccgctt tgatcaattc aagcagaatc tgtacggaaa taccgccctc
     2941 cgctgcgggc agcttgagga tattgatatt gccggtcagc agcccctcca gaacgctgaa
     3001 tgctggaaga ccatccgcat ttcccgccgc aatatgcagc agaactccca gtggaaaggt
     3061 cctctccttt acagcataat tcctgaaact cggtgtaaat gtgttaggac ggctgtaatc
     3121 ctcacccagc tctacactca ggcggtacct caggctatct tcgctgaaca tccggcgcgc
     3181 ttcatcaaga taactttttc ccagcgcttc gtcaattccg atcccggcca tcttctttaa
     3241 atattgggct tcatctaaat ttgatactaa cctgtcgcag gcggatatta ccgtttccgg
     3301 atcaagtctg cccttttgaa aagtacgaag tatcctctgg tcaagctcat ctaatacagc
     3361 gtcgcattcg ccggaactaa taattttgcc atcgactaaa ttcattttgt tccgcccttc
     3421 tgcagcacat tcagtaattc ggacgctccg gccgcacatg tcttgatatc ggccagacca
     3481 acgcgtccca gaatgtcaaa atacggtgaa tgaatcccac agccgcactc ctcgcccgga
     3541 tgcaagacag caagatcgtc ggtcataaca ttgacaaacg gcatactcgt catcatgggc
     3601 gtaatcaggt ttaaaagccc cggctctccg tattttacag gattaagttc agtatcccgg
     3661 atgataaccc tagagtaaac aggcacatga aaatgatgat tcgggcagtc aaaataagct
     3721 atggggtgtt ctaccgcgcc gaaaaattcc cggattcggt cgccgccgat gcccagagtt
     3781 tcctttgaca tttgataaag agtctgctta tctacccgct ccgcaaaaaa ttgcttccag
     3841 ccgcctgcca gaattacaag agatttcgga tgaagcttta aatgaatccg ggagttcata
     3901 agttctttga ttaaaaacat gaaataggcg ggaaatccca taaaccgtac cggaaaaccg
     3961 ctcttttcat aatgaatcaa a
//
LOCUS       DATALY010000373         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_595682,
            whole genome shotgun sequence.
ACCESSION   DATALY010000373 DATALY010000000
VERSION     DATALY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595682"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHR42_10190"
     CDS             <1..323
                     /locus_tag="VHR42_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027573.1"
                     /translation="GGQKQRMTIARALVGSPQILILDDSASALDFATDAALRKSIKNE
                     TAGMTVLIVSQRVSTIRRSDKIIVLDDGAVAGIGTHEQLMESCEVYQEICLSQLSKDE
                     VYKK"
     gene            320..2068
                     /locus_tag="VHR42_10195"
     CDS             320..2068
                     /locus_tag="VHR42_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027574.1"
                     /translation="MRKSVLKRLMRYAKPYSLYLFIALVSAVISISMTLYGPILIGKA
                     IDHIIGTGKVDFPGILPILGILAFTIVLSAVFQWVMAYCTNKVTYQTVRDLRMQTYRK
                     INRLPLKYIDGHAHGDLISRIVNDVDQVSDGLLQGLTQLFTGVVTIIGTLIFMLTISP
                     VITVVVVLVTPLSLFVAGFIAKLSHRQFKEQQATQGELSGYIEELISGQKIVKTFHYE
                     ERSEQQFDEINGRLYRCGVKAQFYSSLSNPSTRFVNGIVYTSVAVIGSVCAVTGRPGP
                     LTIGQISSFLAYANQYTKPFNEVTAVITQIQTAFASAQRLFSTLDEMEEIPDAPDALV
                     LNSCEGNIDVGHLNFSYRPEAKLIQDMTLSARKGDRIAIVGPTGCGKTTIINLLMRFY
                     DADSGTIKIDGINTRNITRSSLRSMFGMVLQDTWLFSGTVRDNIAYGRKDATEEEVIA
                     AAKEAHAHSFIVKLPDGYDTVLSEDGGNLSLGQRQLLCIARVMLADPPILILDEATSS
                     IDTRTEIRIQKAFARMTQGRTSFIVAHRLSTIKEADLILVMNRGSIIEQGTHEQLLAK
                     DGFYANLYNSQFAAAE"
     gene            2205..3050
                     /locus_tag="VHR42_10200"
     CDS             2205..3050
                     /locus_tag="VHR42_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX3027575.1"
                     /translation="MDKTEKRKLQILACKVRMGAIEGVFNAKSGHPGGSLSAADLFTY
                     LYFKEMKIDPKDPKNPDRDRFVLSKGHCAPGLYATLALRGYFSVDELKKLRHIGAMLQ
                     GHPDMKGTPGVDMSTGSLGQGVSAACGMALAGKMDKKGYRVYSVLGDGEIEEGQVWEA
                     AMFAAHHNLDNLCLIVDNNGLQIDGPVAEVGGPEPIDEKFRSFGFDVQIISGNDFDEI
                     ETAFSHAKTVKGKPSVIIAKTVKGKDVSFMENKVNWHGAAPNAEQYEVAMQDLNKVLS
                     GLEAE"
     gene            3051..3995
                     /locus_tag="VHR42_10205"
     CDS             3051..3995
                     /locus_tag="VHR42_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX3027576.1"
                     /translation="MAEMVKKATRESYGQALAELAEQYPQVVVLDADLAEATKTGIFK
                     KAYPDRFIDCGIAECNMVGVAAGLAACGKIPFATSFAMFSAGRAYEQVRNSVGYPKLN
                     VKIVGSHAGISVGEDGATHQCNEDLALMKTIPGMVVLNPADHYEMKAAVKAAIEHHGP
                     VYIRLGRLAVDSFNNADDYKFEIGKGITLRDGKDVTIVATGLMVSRALQAVKTLEEQG
                     IDARLINIHTIKPIDRELILKAAKETGRIITVEEHNVVGGLGDTVCSLLAEELPTPVT
                     KIGVNDVYGHSGPAVDLLDEFGLNAAHIVEVTKKVLGK"
     gene            4318..5526
                     /locus_tag="VHR42_10210"
     CDS             4318..5526
                     /locus_tag="VHR42_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX3027577.1"
                     /translation="MNFNVNELLNSLSFTLDYVEKGLISNEKNHGRRVAYISARIGEM
                     LGFNNLNLFDLISYSLLHDNGVAKSLVKKTDSGEYVNAELNSIHCVEGEKNIKVFPFF
                     HPVTNVLLYHHENFDGSGFFGLSGSEIPLYSRIIALADFIAIQYSEDKVSEEVIRSIE
                     KTKSYFDPDLLSVFEELSHNMEFWLHLNDDFVYTALAAVTPHVNRNFDYKNMRTVSQV
                     FCNIIDAKSPFTGSHSRGISDKTGILCEYYEFDQESYWKMRIAADLHDLGKLMIPNEI
                     LDKPAALTKQEILIIQSHPFYTRKALEMITGFEEIADCAANHHEKLNGSGYPFGLNQD
                     QLDYKSRLLGCIDIYQALTEDRPYRKAMAHNIAIRIMREMASKNLIDQSIVEDLNFVF
                     SVTSKFAVSK"
     gene            5596..5739
                     /locus_tag="VHR42_10215"
     CDS             5596..5739
                     /locus_tag="VHR42_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027578.1"
                     /translation="MSYVDPKLRSRFESLSIDLKNEILKRDVKLYTLNDLIKCLEDIV
                     SGK"
     gene            5933..6196
                     /locus_tag="VHR42_10220"
     CDS             5933..6196
                     /locus_tag="VHR42_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027579.1"
                     /translation="MLLHKIEAEVVPMLCDLSPEELSYLATTFAVTLAKGLDDHSMRV
                     LCSFFVDVVGTLNLITNQRSLLEKCHEKCEEHHEKPEKPHDRK"
BASE COUNT         1656 a         1523 c         1534 g         1497 t
ORIGIN      
        1 ccggcgggca gaagcagcgg atgaccatcg cacgcgcact ggtgggcagc ccacagattc
       61 tgattctgga cgacagtgcc agcgcgctgg actttgcaac tgacgccgca ctgcggaaat
      121 ccattaaaaa tgaaacggca ggcatgaccg tgctgatcgt ttcccagcgg gtaagcacca
      181 tccggcgctc ggataaaatc atcgtactgg acgacggggc ggtcgcggga atcggcacgc
      241 acgaacagct gatggaaagc tgcgaggttt accaggaaat ctgcctctcg cagctcagca
      301 aagacgaggt gtacaaaaaa tgagaaaatc cgtattaaag cgtctgatgc gttatgccaa
      361 gccgtattcc ctctatcttt ttatcgcgct tgtcagcgcg gtaatcagta tctcaatgac
      421 attgtacggc cccattctca tcggtaaagc gatcgaccat atcatcggta ccggaaaggt
      481 ggattttccg ggaatcctgc caatccttgg aatacttgcc tttacgatcg tactgagcgc
      541 cgtatttcag tgggtaatgg cctactgcac aaataaggtt acctaccaga cagtgcgcga
      601 cctgcggatg cagacctacc ggaaaatcaa ccggctgccg ctgaaatata ttgacggaca
      661 cgcccacggc gacctgatca gccggatcgt caacgacgtc gatcaggtat cggacggcct
      721 gctgcaaggc ctgacgcagc tttttactgg cgtcgtcaca ataatcggca ctctgatttt
      781 catgctgacg atcagcccgg tcatcacggt tgttgtggtg ctggtcacac ccctgtcgct
      841 tttcgtcgcc ggttttatcg caaagctttc ccaccggcag tttaaggagc agcaggcgac
      901 gcagggcgag ctgagcggct acattgagga gttgatcagc ggtcagaaaa tcgtaaagac
      961 cttccattat gaagaacggt cggaacaaca gtttgatgaa atcaatggac gtctttaccg
     1021 ctgcggcgta aaagcccagt tctactcctc cctcagcaac ccgagcacac gctttgtcaa
     1081 cggaatcgtc tatacctccg tggcggtgat cggttccgtc tgtgcggtga ccgggcgccc
     1141 ggggccgctg accatcgggc aaatatcttc tttcctcgcc tacgccaacc agtacaccaa
     1201 accgttcaat gaggtcaccg cagtcattac gcagattcaa accgcatttg cctccgccca
     1261 gcggcttttt agcacccttg acgaaatgga ggaaattccc gacgcgccgg atgcactggt
     1321 gctcaatagc tgcgagggca atatcgacgt agggcatctg aacttctcct accgtccgga
     1381 ggcgaagctg attcaggata tgaccctctc cgcccgcaaa ggcgaccgca ttgcaatcgt
     1441 cggcccgact ggctgcggca aaaccaccat catcaatcta ctgatgcgct tttacgacgc
     1501 ggacagcgga accataaaaa ttgacggcat caatacccgg aacatcacca gaagcagcct
     1561 gcgctccatg tttggaatgg tactgcagga tacatggctg ttttccggca ccgtgcgtga
     1621 caatatcgcc tacggcagaa aagacgcgac agaagaagag gttattgcgg cggcaaaaga
     1681 ggcgcacgcc cattccttta ttgtaaagct gcccgacggc tacgacaccg ttctttccga
     1741 ggacggcggc aatctttcgc ttggccagcg ccagcttctc tgcatcgcac gggtcatgct
     1801 ggcggacccg ccgatcctga ttctggacga ggcgaccagc tccatcgata cccgaaccga
     1861 aatccgtatc cagaaagcgt tcgcacggat gacgcaggga cgcaccagct ttatcgttgc
     1921 ccaccgtctt tccaccatca aagaagccga tctcatcctt gttatgaacc gcggcagtat
     1981 tattgaacag ggcacccacg aacagcttct ggcaaaggac ggtttttacg caaaccttta
     2041 caacagccag tttgcggccg cggagtaaaa actaaaggga gatgtcgacc gcagtcgaca
     2101 gagagagtaa gcgactttat gcgattcaat tccttcttta gtattgaatt accagatagt
     2161 gcgttgtata attatataga attaacgaaa atcaggaggt cagcatggat aaaacagaga
     2221 agagaaaatt gcagattctg gcttgcaagg tcagaatggg tgcgatcgag ggagttttca
     2281 atgcaaaatc agggcacccg ggcggatcgt tatccgccgc ggatctgttc acctatctct
     2341 atttcaaaga gatgaaaatt gatccgaagg accccaagaa tccggacagg gaccgtttcg
     2401 tcctttccaa aggccactgc gcgcccggac tttacgcgac actggcttta agaggttact
     2461 tttcggtaga tgaactgaag aaactgcgtc atatcggggc aatgctgcag gggcatccgg
     2521 atatgaaggg tacgccaggc gtcgatatga gcaccggttc cctcggccag ggcgtttccg
     2581 ccgcctgcgg gatggcgctt gcgggcaaga tggacaaaaa aggctaccgt gtatattcgg
     2641 tcttgggcga cggcgaaatt gaagaaggac aggtttggga agccgcaatg tttgccgcac
     2701 accacaacct ggacaacctc tgtctcatcg ttgacaacaa cggtctgcag attgacggcc
     2761 cggtagcgga agtcggcggc cccgaaccga tcgacgaaaa attccgttcg tttggctttg
     2821 acgttcagat catcagcggc aatgatttcg acgagatcga aaccgcgttc agccatgcca
     2881 agactgtaaa aggcaagccg agcgtgatta ttgcaaagac cgttaagggc aaagacgtat
     2941 cattcatgga aaataaagtg aactggcatg gcgccgctcc caacgcagag caatacgaag
     3001 ttgcgatgca ggacttgaac aaggtgctgt ccggactgga ggcggaataa atggctgaga
     3061 tggtaaagaa agcaaccaga gagagctacg gccaggcgct ggctgaatta gccgaacagt
     3121 acccgcaggt tgtcgtttta gacgcagacc ttgcggaggc aaccaaaacc ggaattttta
     3181 agaaagctta tccggatcgc tttatcgatt gcggcattgc ggaatgcaac atggtcggtg
     3241 tggccgccgg tcttgcagcc tgcggcaaaa tcccgtttgc aacttccttt gcaatgttct
     3301 ctgcgggccg cgcctatgag caggttcgca attccgtggg atatccgaag ctgaacgtaa
     3361 aaatcgtggg ttcccacgcc ggtatttccg tcggcgagga cggagccacc catcagtgca
     3421 acgaagacct tgcgctgatg aaaacaattc ccggcatggt cgtgctgaat cctgccgacc
     3481 attatgaaat gaaagcagct gtcaaagcgg cgatcgagca tcacggcccg gtttatatcc
     3541 gtcttggccg tcttgccgtg gatagcttca acaacgccga cgattacaag ttcgaaatcg
     3601 gtaagggtat taccctgcgc gacggcaagg atgtcacgat tgtggcgacc ggcctgatgg
     3661 tatcccgtgc gctgcaggct gttaaaacgc tggaggagca gggaattgac gcgcggctga
     3721 tcaacatcca caccattaaa ccgatcgacc gtgagctgat cctcaaggcc gcgaaggaaa
     3781 ccggccggat catcaccgtg gaagagcaca acgtagtcgg cggcttgggc gataccgtgt
     3841 gcagtctgct tgccgaagag ctgcccaccc cggttaccaa aatcggcgta aacgatgttt
     3901 acggccattc cggtcccgca gtcgacctgc ttgacgagtt tggcctgaac gcggctcaca
     3961 ttgttgaggt taccaaaaag gttcttggca aataaattct aagtttagct taatatgcta
     4021 ccgcgaaccg gcccgctttt cggcgggtcg gtttattaat ttaagtaata atcagacaat
     4081 ttaagttgaa ttgcggcata aatcaggatc aaatattaat ttctatcatg aaattaaaaa
     4141 aagtttctgt tttctgcaaa tttaattgtt attattcctt ttagttatta aatatagtgg
     4201 ccagagtagt taaaatatat ttaagtaaat tacagtaatt gacgtattaa aattccaata
     4261 actgtttatt ccatcttaca attaacttcg ttacaatact atctggagat tacgaaaatg
     4321 aattttaatg taaacgaact actgaattca ctctccttta ctctcgatta cgtagaaaaa
     4381 ggcctgattt caaacgaaaa aaatcacggc cgccgcgtcg cgtatatatc tgccaggatt
     4441 ggggaaatgc tcggtttcaa caatctcaat ctattcgatt tgatttccta ctcccttctg
     4501 catgacaacg gggttgcaaa atctcttgtg aaaaagaccg attcggggga atatgtaaac
     4561 gcggagctga acagcatcca ctgcgttgag ggagaaaaaa acataaaggt atttccgttt
     4621 tttcatcctg ttacaaacgt acttctctac catcatgaaa atttcgacgg cagcgggttt
     4681 tttgggttat ccggcagtga aatccctctg tattcccgca ttatagcact tgcagatttc
     4741 attgccatac aatattcgga agataaagta agcgaagagg tcatccggtc aatcgaaaaa
     4801 acaaaatcgt attttgaccc ggatttgctc agcgtctttg aggaactcag ccacaatatg
     4861 gagttctggc tccatttgaa cgatgatttt gtttataccg cgctggcagc ggttaccccg
     4921 catgtcaacc gtaattttga ttacaagaac atgagaaccg tttctcaggt tttttgtaat
     4981 atcattgatg ccaagtcacc ctttaccggg tcgcattcgc gcggaatcag cgacaaaacc
     5041 ggcatcctct gcgaatacta tgagtttgac caggaatcct actggaaaat gcgcatagcg
     5101 gcagacctgc acgatcttgg aaagctgatg attccgaacg aaatactcga caaaccggcc
     5161 gctctgacaa agcaggaaat tttgattatc cagtcccatc ctttttacac ccggaaggcg
     5221 ctggaaatga tcaccggatt cgaagaaatc gccgattgtg ccgcgaacca tcacgaaaaa
     5281 ctgaacggca gcggctatcc tttcggcctg aatcaggatc agctggatta taaatcccgt
     5341 cttttaggct gcatcgatat ttatcaggcg cttaccgagg acagacctta ccgcaaagcg
     5401 atggctcata acatcgcaat tcgcattatg agggaaatgg cttcgaaaaa tctgatcgac
     5461 caatcgatcg ttgaggattt gaactttgtc ttttccgtga catcaaaatt tgcagtttca
     5521 aaataatttt tcaaccgccc ttaccgggcg gttttttttc ggagaaacgg gaaaactgtt
     5581 tttgaggtga taggtatgtc ttatgttgac ccgaagctgc ggagccgctt tgaatcgctc
     5641 tccattgatc tgaaaaacga aattctcaaa agggacgtta agctgtacac gctgaatgat
     5701 ttaataaagt gtctggaaga tatcgtatcc ggtaaataaa tgttaaaaat gtataaattt
     5761 taacattatt ctatagattc tctaaaaact gaatttattt gttatactaa tttatagttt
     5821 acaagttcct cacgcgagga ttttcaataa ccttttgaaa aagccgtcca ttattgggcg
     5881 gctttttcaa tgatattcct ttttcgtacc gtttgccgca gtacaacggg atatgctgct
     5941 gcataaaata gaagcagagg tggttccgat gctttgtgac ctttcccctg aagagctgtc
     6001 ctatctggca acaacgtttg ccgtaacgct tgcaaaaggc ctggatgacc actccatgag
     6061 agtgctgtgc agctttttcg ttgacgttgt aggaactctt aacctcatta caaatcagcg
     6121 gtctttgttg gaaaagtgcc atgaaaaatg tgaagagcat catgagaaac cggaaaaacc
     6181 gcatgatagg aaatgaacat cctccggggt
//
LOCUS       DATALY010000374         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_595844,
            whole genome shotgun sequence.
ACCESSION   DATALY010000374 DATALY010000000
VERSION     DATALY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595844"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..1110)
                     /locus_tag="VHR42_10225"
     CDS             complement(331..1110)
                     /locus_tag="VHR42_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX3027580.1"
                     /translation="MDYGLTVLIGIAVLVILLLSLSLVVINEYERGVVFRFGHVIGVL
                     GPGLKLRIPFGIDIVRKVDLRTRTIDVPQQEIITKDNISIKVDAVVYYHIQDPVLVIT
                     KVVNFNSSTTMLGQTTLRSVLGQHELDEILTSKDNINTILKNMLTETMSSWGIIITAV
                     ELRSIEIPDSMKRAMAKQAEAERERRAKVTAAEGEAQAAAKLYEASEVIRKSPSALQL
                     RYLQTLTEIAVEKNSTIVFPLPMEFMRAFGKKVEPEQPEEK"
     gene            1306..1554
                     /locus_tag="VHR42_10230"
     CDS             1306..1554
                     /locus_tag="VHR42_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027581.1"
                     /translation="MTKEPEEIKVLTKDTRETKEAICLLNGENVSIDHRCLEIPGRPG
                     DRAFLRIDSWNCTKAESCGNPDCVGRVRTGIVRNWKEQ"
     gene            complement(1638..2768)
                     /locus_tag="VHR42_10235"
     CDS             complement(1638..2768)
                     /locus_tag="VHR42_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3027582.1"
                     /translation="MDFQYYLPVNLIFGRGKISKLGTETAQYGKKALLVTGMSSTKKT
                     GLLAKAETILKEAGVSSVVFDRVEPNPLTTTVHTGAKLAREECCDVVVGLGGGSIMDA
                     AKAIAFMAVNSGDVSDYVFGRKTSTEALPLILVPTTCGTGSEGNEFAVLSNPENHDKK
                     SLRTMPIVAKTSLIDPELMMTMPKGVLASVGFDALCHNMEAFLSASGQPISNVLAQEG
                     IRLMAEYLPKVYGGSSDPSDWEKVTMGSTIGGMVISIASVTAPHGMEHPASGLRNIVH
                     GRGLAALTPVIFEESISSAPEKFSVISKLLGGKDEKDCADQVRKFLTKIDLTQSLGDQ
                     GVLESDIDWMAQNCLKVSVGSMGNHPIVFGQEDIRRIYKKAL"
     gene            complement(2922..>3651)
                     /locus_tag="VHR42_10240"
     CDS             complement(2922..>3651)
                     /locus_tag="VHR42_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HEX3027583.1"
                     /translation="KGAAVQAFENVLASLNERYKAYCTLSGTAFQPINYPHRVLEYAE
                     LFRMAQRHFGGELERETQNMASELLEKGEDKRFIPLRIAERLCEIAEVKTPTIVVFFA
                     APYCPHNTLKAEVPEEKALLGKISKLVSAFGKETGENYRVMQFFPSLSDSSYLKIDDD
                     AQSLETLMRNFPAYSRIYPLPFDRIRLLNLAGLNYGCFGKDAHKWTERVYLPYSFEIF
                     PKLILKTVEEFLMNGTEDFQGRHD"
BASE COUNT          894 a         1008 c          869 g          880 t
ORIGIN      
        1 acatacagca gccgcttttt tataaaacgt ttccggacaa aatcatttaa cacacttcct
       61 aaaaacagca gcagaaagaa cacctttccg ggtgcgtttc cgtaggctat tcccgcgaag
      121 gccatagcga ttatgacgag catgacaatc cgaagatatc ggccgtaatt tttcataggt
      181 catcccattg aaacagtctt cttatcattt tacccgaatc tcacggccaa aagcaacaaa
      241 ccgtgaaaag ccggactggc gccgctcgaa tgaacggcgc cagtccgctg aaccctgaat
      301 tttccgcttt catgcttcga atagcagagc ttatttttct tccggctgtt ccggctcaac
      361 ttttttgccg aaggcccgca tgaactccat aggcaatggg aaaacgatgg tggaattttt
      421 ttccaccgca atttccgtca acgtctgcag ataacgcaac tggagagccg aaggcgattt
      481 ccgaatcact tcggaagctt cataaagctt cgcggccgcc tgcgcttcgc cctcggccgc
      541 agtgaccttt gcacggcgtt cccgctcggc ttcggcctgc tttgccatgg cgcgcttcat
      601 ggaatccgga atctcaatgc tgcgcagttc gactgccgta atgatgattc cccaggaaga
      661 catggtctcc gtcagcatat ttttaaggat tgtattgatg ttgtccttgc tggtcaggat
      721 ttcatccagc tcatgctggc ccagcacgga gcgcagagtc gtttgcccca gcatcgtggt
      781 gctgctgttg aaattgacca ctttcgtaat aacaaggacc gggtcctgaa tgtgataata
      841 aaccacggca tctactttta tggaaatatt atcctttgta ataatttcct gctgcggcac
      901 atcgatggtg cgcgtgcgaa gatcgacctt ccgcacgata tcgatgccga acggaatccg
      961 cagcttcagg ccgggaccaa ggaccccgat tacgtgtcca aagcgaaaaa ctactccccg
     1021 ctcatattcg ttgatgacaa ccagagacag tgaaagcagc agaataacca atactgcaat
     1081 accgatcagc acagttaagc cataatccat ctttaacatc ccactttccg cgcacagggc
     1141 gctgtatgaa tcacaatagc agcaatccgt gaggatcaag gttattgtat ccctgttatg
     1201 tcggcgatgc aagagaattt ctgaaaaagt atgctgcccc gacattgact tttcttttcc
     1261 gattgtacta tgataaacac agtaaacaaa aaggagagaa cagccgtgac aaaagagcct
     1321 gaagaaataa aagttttgac caaagatacc cgcgaaacaa aagaggccat atgccttttg
     1381 aatggtgaaa acgtttccat cgaccaccgt tgccttgaga ttcccggcag accgggagac
     1441 agagcctttc tccgtatcga cagctggaac tgtacaaaag cggaaagctg cggaaatcct
     1501 gactgcgtcg gccgggtacg gacggggatc gtgaggaact ggaaagaaca ataattttcg
     1561 aagagcaatc aataagccct catcggtcag ccgggttacg gctgtccgac gggggctttt
     1621 ctatatctgt attaaaatta aagcgctttc ttgtaaatcc ggcgaatatc ctcctgcccg
     1681 aacacaatcg gatgattccc catggagccg accgaaacct tcaggcagtt ctgcgccatc
     1741 cagtcgatgt ccgactccag cacgccctgg tcccccaggc tctgcgtcag gtcgatcttt
     1801 gttaagaatt tacgaacctg atccgcgcag tctttctcgt ctttgccgcc cagcagcttt
     1861 gaaatcaccg aaaatttttc aggggcgctg ctgatggatt cctcaaaaat gaccggcgtc
     1921 agtgccgcca gaccgcgccc gtggacaata ttccgcaggc cgctggccgg atgctccatg
     1981 ccgtgcggcg ccgtcacgct ggcaatggaa atcaccatgc cgccgatggt actgcccatc
     2041 gtgacctttt cccagtccga agggtcagag ctgccgccgt atactttggg aagatattcc
     2101 gccatcaggc gaatgccctc ctgagccaga acattggaaa tcggctgacc ggaagcggaa
     2161 aggaatgcct ccatgttatg gcacagagca tcgaatccca ccgaagccag cacgcccttc
     2221 ggcatggtca tcatcagttc cgggtcaatc agagaggtct tggcaacaat cggcatcgtg
     2281 cggagcgact ttttatcgtg attttccggg ttcgacagaa ccgcaaattc attaccctcg
     2341 ctgcccgtgc cgcaggtggt cggtacgaga atcagcggca gtgcctccgt tgacgttttt
     2401 ctgccgaaca cataatcgga aacatcgccg ctgtttacgg ccataaaggc aatcgctttt
     2461 gcggcgtcca tgatgcttcc cccgccaagg ccgacaacca cgtcacagca ttcctcccgc
     2521 gccagcttcg cgccggtatg gacggttgtg gtaagaggat tcggttcaac acggtcgaaa
     2581 acgacggagc ttacaccggc ctcttttaaa attgtttcgg cttttgccaa cagccccgtt
     2641 tttttcgtac tggacattcc tgtaaccaga agcgcctttt tcccgtactg ggcggtttcg
     2701 gttcctaatt tggaaatttt ccctctgccg aaaatcaaat ttacaggaag ataatactga
     2761 aaatccatgt tgtttctcct ctctgttgat aaagctccgc cgtacgcaag taccggttcc
     2821 gcgctcagat cggcctaagc ccgaagacat gcccggcggc ggtagattat ttgaatctga
     2881 agctatactg cgtaaagtat gcaccttttt gcatacgatt gtcaatcgtg ccgcccctga
     2941 aagtcctcag ttccattcat caaaaactct tccactgttt tcagaatcaa ttttggaaaa
     3001 atttcaaacg aatacgggag atacacccgc tccgtccatt tatgcgcgtc tttaccgaag
     3061 cagccgtaat tcaggcccgc caggttcaac aaacggattc ggtcaaaggg cagcgggtaa
     3121 atccggctgt atgccgggaa attgcgcatc agcgtttcca gggactgcgc gtcgtcgtcg
     3181 attttcagat aactgctgtc ggaaaggctg ggaaagaact gcatcacacg gtagttttct
     3241 ccggtttctt ttccgaaggc cgacaccagc ttcgaaattt tgccgagcaa agccttttct
     3301 tccggcacct ccgccttcag ggtgttgtgc gggcaatagg gagcggcaaa aaacacgacg
     3361 atcgtcggag tcttcacctc agcgatttca cacaggcgct cggcaattct aagcgggatg
     3421 aagcgtttgt cttccccctt ttccaacagc tccgacgcca tattctgcgt ctcccgttcc
     3481 agttccccgc cgaagtgccg ctgtgccatg cggaacagct ccgcatattc gaggacccga
     3541 tggggatagt taatcggctg aaacgccgtt ccgctcagag tgcagtaggc tttataacgt
     3601 tcattgagcg aagccagaac attctcaaac gcctgaacag ccgctccctt c
//
LOCUS       DATALY010000375         8839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_257739,
            whole genome shotgun sequence.
ACCESSION   DATALY010000375 DATALY010000000
VERSION     DATALY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257739"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VHR42_10245"
     CDS             complement(<1..618)
                     /locus_tag="VHR42_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX3027584.1"
                     /translation="MRKIRILTDSASDIPLKIAERLDIKVLPIPITHDGKSYHERVEF
                     SNEQFYQLLLESRVVPFTSHINANTYLEEYKKAYEQGYTQILNVTINSLGSSMFDSAN
                     LAKRMFYEETGVAEDRMRIEVMDSKTYTIAYGLAVIRAAEMADRGIELDDIVAYLSDW
                     FDRLEIMFSVYSLDFVKKSGRVGCAAAFVGELLGLRPVIVFVDGVA"
     gene            complement(622..1152)
                     /locus_tag="VHR42_10250"
     CDS             complement(622..1152)
                     /locus_tag="VHR42_10250"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX3027585.1"
                     /translation="MEYSQKGEIFLPVLNIVLVEPEIPQNTGNIARTCAATGARLHLV
                     KPLGFQIDDRKLKRAGLDYWWMLDITFYESLDDFFRKNNGPFYYFTTKARNVYSDIAY
                     PDGSYLVFGKETAGLPEELLVNNKENAVRLPMRDEARSLNLSNSVAVGVYEVLRQWEF
                     PDLKEQGNLTRFNWEE"
     gene            1324..1869
                     /locus_tag="VHR42_10255"
     CDS             1324..1869
                     /locus_tag="VHR42_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HEX3027586.1"
                     /translation="MYFESAGRQNTEKTIELTLRTARERKIEHLVVASTGGDTPRLLN
                     PKGLNVVCVTHAYGSPKPGENDMPEDIRKQLSDNGIKLLTATHVLSGAERGISKEFGG
                     IYPVELIAHTLRMLGQGTKVCVEIAVMALDAGLIPYHTPVIAMGGSGKGADTAVILTP
                     SHANDIFETKIHEYICKPYNV"
     gene            complement(1948..3126)
                     /locus_tag="VHR42_10260"
     CDS             complement(1948..3126)
                     /locus_tag="VHR42_10260"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HEX3027587.1"
                     /translation="MYDFEMIVDRRGCGSYKWDAIAAEMGEGAEDVTPMSVADMELKT
                     APEITEALKKAVDFGVYGYTGPTEGYYEAVQGWMERRHGWKIKKDWISLSPGVVSAFY
                     NAIRALTHPGDKVIIQRPVYYPFSSAVKLSGCEIVNNPLILKDGRYEMDFEDLEQKAK
                     DPRVKVLLFCNPHNPVGRVWTAEELKKLGEICLKNNVVVVSDEIHFDFVFKPFHHTVF
                     ATISPEMEQNCMILTAPSKTFNLAGLQCSNIIIPNPELKAAFDLAFENTGTFSLNYFA
                     YPACEAAYRSGEGWLDGLLELLESNKKILADFLADELPQIKLIEPEGTYLAWLDCRGL
                     KMNRDELETFMKQKARVFFDEGYLFGEEGNEFERINLACPEKMLKKVLENIKAAIKRT
                     "
     gene            3790..4548
                     /locus_tag="VHR42_10265"
     CDS             3790..4548
                     /locus_tag="VHR42_10265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3027588.1"
                     /translation="MKALITGASSGIGRDMARVLSRRGYDLILVARRRDRLEELAREL
                     PVKVRIICLDLSGEKSCRELHDLTAEEKIDILINNAGFGLFGTFQETDLEKELDLIDL
                     NIRAVHILTKLFLQDFIKRDSGYLLNVASSAAFLPGPLMSSYYASKSYVFRLTEAIYE
                     ELRRSGSRVSVSVLCPGPVKTEFEKTADVNFTIQKLESSRVAEYAIDRMLRRKLVIIP
                     GAIMKTARFFERFLPEKALLRAAWHMQERNMNGD"
     gene            4554..4952
                     /locus_tag="VHR42_10270"
     CDS             4554..4952
                     /locus_tag="VHR42_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027589.1"
                     /translation="MKKTSLLRSFLRFGLPVCLVSFSLLLIGIRFAAGNQMNSKIILS
                     CFILSLIFGAVSFALFRFRIWMLCFWAGLAIGFFDMFRIFIDGMEGWGDLIGLFSLFI
                     WAIAGILGGAAAQLLRFLLRKFTGRRTRRE"
     gene            complement(4957..6141)
                     /locus_tag="VHR42_10275"
     CDS             complement(4957..6141)
                     /locus_tag="VHR42_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3027590.1"
                     /translation="MNQLEQRADKIAEEMKGTIEEISDYIFNHPELGDREYRSSEYLS
                     GLLADHGFNVETPYLGLETAFRAEFGDGEGPKIAFLPEYDALPGYEGGAAHACGHNWI
                     AASTIGAALILSKMKDAFRGKVVVIGTPAEETSGRKVDLTEKGAFDDVDAVFQLHLYQ
                     NNNFNAKALAIDSWEFEFKGKASHAAAFPYDGINALDAVNLTFAGIGALRQQLRQDVR
                     IHGVITKGGEAPNVIPDLCSCKFYVRSEKRAGLDDVSEKVKNCARGAALMTGTKLEIR
                     QFENSYDDLVLNSVLVELMKDQLRRAGIENFSDEIENAGSTDIGNVSYRVPTVYGNIG
                     VGDGSVNVHEAAFLTLANGREAKQKLLIAARAFAGAALELFSRPELMKEAKEEFEHSV
                     GR"
     gene            complement(6337..6990)
                     /locus_tag="VHR42_10280"
     CDS             complement(6337..6990)
                     /locus_tag="VHR42_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3027591.1"
                     /translation="MSILTIEHLNYSYDNGKTPVLKDINFEFEKGKTYSIVGKSGSGK
                     TTLLSLMSGLENSKSGKILYDGSDIAKIDREKYRSQNIGVIFQSFNLLPHLTALENVV
                     LSMDVAGMKLNDKKKHALDILEKVGLNAEKAGRRVLKLSGGEQQRVAIARALSYNPDI
                     ILADEPTGNLDGGTEAEVMKILKGLAANDNKCVIIVTHSPNVSKNTDCVYSLEPIAI"
     gene            complement(7007..8515)
                     /locus_tag="VHR42_10285"
     CDS             complement(7007..8515)
                     /locus_tag="VHR42_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3027592.1"
                     /translation="MYIVKNAWKSISRSKGRNILIGIIVLVIAASSCVALAIRSSANE
                     VVRQQKESNTITATIGVNREAIQKQAQSSSSDMRTIMNSVPSLTLAQLKKYADSSYVK
                     SLEYQISLSLDSTNITAASNTSTSSTGSGSSSAAKQSGVEGGQAPGGNFSEQSQGDFQ
                     LVGYSSTSAMTDFVSGTYKIKSGSMFSDSDTTNDCVISSDLAQANSLKVGSQIILTNP
                     NDATQKVTYKVVGIYTDTTSSDGSQMNLFSNAANKILTNYTEANKIVTAASGSSTTKL
                     ESRLSSSFTLKSASSVDSFEAELKTKGMSSYYSVTTNEDSVTSSLEPLKNLSNFAGIF
                     LILVLAIGGIILVVLNMFNIRERKYEVGVLRAIGMKKGKVALQFVTELFIVTFAALTI
                     GAAAGSAASVPVANYMLKNQISSIQSQQTQVAQNFGRPGSGGGGMGGSGIGGFFGTSK
                     NVSYVNQINAVISGRVLLQMAGIGILLTIISSGISMVFISRYEPLKILSSRS"
     gene            complement(8633..>8839)
                     /locus_tag="VHR42_10290"
     CDS             complement(8633..>8839)
                     /locus_tag="VHR42_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027593.1"
                     /translation="IFFKLCLIILSYCGWFVNLIFAVVRIKSTIFRGHNRQRAFTGAF
                     HNSMPAPDQMDRAAGYDYGFESSR"
BASE COUNT         2140 a         2284 c         2098 g         2317 t
ORIGIN      
        1 agccaccccg tcgacaaaaa caatcacagg acgcagccca agcagctcgc cgacaaatgc
       61 tgcggcgcag ccgaccctgc cggatttctt cacaaagtcc agggaataaa cggaaaacat
      121 aatttccagc cggtcaaacc agtcggaaag atacgcgacg atgtcgtcaa gctcaatgcc
      181 ccggtcggcc atttcggccg ccctgattac ggccagccca taggcgatag tgtaggtttt
      241 ggaatccatg acctcaatgc gcatgcggtc ttccgcgacg cctgtttctt cataaaacat
      301 ccgtttggca agattggctg aatcaaacat gctgctgcca agcgaattga ttgtaacatt
      361 tagaatctgt gtatagccct gctcataagc ctttttatat tcttccagat atgtattggc
      421 attgatatgc gacgtaaacg gaactaccct gctttccaga agaagctgat aaaactgctc
      481 gtttgaaaac tccacccttt catgatacga ctttccgtca tgcgtgatgg ggatgggcaa
      541 aacttttatg tcaagccttt cagcgatctt gagcggaata tcgctggctg aatccgtaag
      601 gatacggatt ttccgcattc ctcattcctc ccagttaaat cgtgttagat tgccttgttc
      661 cttcaggtca ggaaattccc actgccgaag cacttcataa acgcccacgg caaccgagtt
      721 tgaaagattc aggctgcgcg cctcatccct catcggaagc cgcactgcgt tttccttgtt
      781 gtttacaagc agctcttccg gcaggcccgc cgtttctttg ccaaagacca gatagcttcc
      841 gtcggggtag gcaatgtcgg aataaacatt cctggctttt gtagtgaaat agtaaaaagg
      901 accgttgttt ttacgaaaga aatcatccag actttcataa aaagtaatat caagcatcca
      961 ccaataatcg aggcccgctc ttttgagctt gcggtcgtcg atctgaaatc ccagaggctt
     1021 gacaagatgc agtcttgcgc cggtcgcggc gcaggttctc gcgatgttgc cggtgttctg
     1081 cggaatttcc ggttcgacca gcacaatatt cagaaccggc aaaaagatct cccctttttg
     1141 agaatattcc atataatttt acatcaaagg gacatttttt tcaagtgtgg gttttgaagc
     1201 gatcccgttc gaatggattc ggccgcaaaa gtggaactta tccataaaat ttatgcctct
     1261 cgcctcgggc ggttatgata taatgaaaag agtgatcgat ttgacgataa aggggaaaga
     1321 aacatgtatt ttgaatctgc gggcagacaa aacaccgaaa aaaccattga gcttacgctg
     1381 cgaacggccc gggaacgcaa aatcgaacat cttgttgtcg cttcgacagg cggagatacc
     1441 ccaaggcttc taaacccaaa aggcctgaat gttgtttgcg tcacacacgc ttacggctcc
     1501 ccgaaacccg gagaaaacga tatgccggag gatatccgaa agcaactgtc tgacaacggg
     1561 atcaaactgt tgaccgccac tcacgtgctg agcggagccg aacgcggtat cagcaaagaa
     1621 ttcggcggaa tctatccggt ggagctgatt gcgcataccc ttcgtatgct cggccagggc
     1681 accaaggtct gtgtggagat cgcggtcatg gcgcttgatg ccgggctgat cccttatcat
     1741 actccggtga tcgcgatggg cggctcgggc aagggcgccg acacagccgt cattctgaca
     1801 ccctcccacg ccaatgacat ttttgaaacg aagatccatg aatacatttg caagccctac
     1861 aacgtataac tcctgcaatt caaataaaca agaggccccc cgctgaaagc gaggggcctt
     1921 cgctgtcaca gaccgtcatg gtgtacttta tgtacgcttg attgccgctt ttatgttttc
     1981 cagaactttt ttcagcattt tttcggggca ggcgaggttg atacgctcaa actcgtttcc
     2041 ttcctcccca aaaaggtatc cttcgtcaaa aaacactctt gctttttgtt tcataaaggt
     2101 ttccagctca tctcggttca tcttaagccc gcggcagtcg agccatgcaa ggtaggtccc
     2161 ttccggctca atcaatttga tttgcggcaa ttcatcggcc agaaagtccg caagtatttt
     2221 cttatttgat tccagcagtt cgagcagacc gtcaagccat ccctcacccg accggtaagc
     2281 ggcttcgcag gcgggataag caaaataatt aagcgaaaag gtcccggtat tttcaaatgc
     2341 gagatcaaaa gccgccttga gttccgggtt gggaataata atattggaac actgaagccc
     2401 ggccagatta aaggttttgg agggagcggt cagaatcata cagttctgct ccatctccgg
     2461 ggaaattgta gcgaaaacag tatgatggaa tggtttaaaa acgaaatcaa aatgaatctc
     2521 gtcggacacc accaccacat tgttttttaa gcagatttca cccagcttct ttaattcctc
     2581 cgccgtccac acccgtccga ccgggttgtg gggattacag aacagaagca ccttgacccg
     2641 agggtctttc gctttttgtt caagatcctc aaaatccatt tcatagcgcc cgtcttttag
     2701 aatcagcgga ttgttgacaa tttcacagcc gctcagtttc acagcgctgg aaaacggata
     2761 gtaaaccgga cgctgaatga tcaccttatc gcccggatgg gtcagcgcac gaatcgcgtt
     2821 ataaaaagcg gataccacgc cgggggacag agatatccaa tcctttttga tcttccagcc
     2881 atgcctccgt tccatccatc cctgaacagc ctcataatag ccctccgtcg gaccggtata
     2941 accataaacg ccgaaatcaa ccgccttttt tagggcttcg gtgatttccg gggccgtttt
     3001 cagttccata tcggcgacag acataggggt gacgtcttcc gctccctccc ccatctcagc
     3061 cgcgatggca tcccatttat aggagccgca gcctcgcctg tctacaatca tttcaaaatc
     3121 atacatagac ttttcctcct gctcatattt gataattttc tattatttta ataattcttt
     3181 accatcaata gaatacacga ttctttcggc tttttcaaga gtccaaagac ggccgaaaac
     3241 atttttggcc atgaaaggtg aaacaatccc agcccgtccg aataaaatga agtgtaccgc
     3301 caaggaggtc ttggaatgag aaacatcgtg cctttattag tacttggctg ccttctaaat
     3361 aattcaggac accggggctg cggcccgcaa ttttgggata actgtaattc gggctgctgg
     3421 gattccgact gccggtctgg ctgcgggttt ggctgccggc cggactgcga gcgtgtctgg
     3481 gaaccctgta acagcccgag gtgcagaggt gtcggctctt gctgcaggcc atgcggccgg
     3541 tgggagagcc catggttccc cggggattcc tgccgcggct catcctgcag aagcttttgg
     3601 aactggtaaa aaggcagagc cctttgccat aggcaaaggg ctcttttatg catgaatgcg
     3661 gccgctttgg caatgcggtt gttttctgaa atttttcgtg ttatattggt aataaacaaa
     3721 atgaaatatc gtgcgaacgg cgagtttcag ccacgcggca caacaaacaa tgaaaaagag
     3781 gcggtatcaa tgaaggcgct gataacgggg gcaagttcag ggatcggcag ggatatggca
     3841 agggtgttga gccgaagagg atacgacctg attcttgtcg cgcgccgcag ggaccgtctg
     3901 gaagaattgg cgcgggaact gccggttaaa gtaagaatca tctgcctgga tctatccggc
     3961 gaaaaatcct gccgggagct tcacgatctg acagcggaag aaaagatcga tattctgatc
     4021 aacaacgcgg gattcgggct ttttgggacg tttcaggaaa ctgatcttga aaaagagctg
     4081 gatctgatcg acttaaacat cagggctgtt cacatcctga caaaactgtt tttacaagat
     4141 tttataaaaa gggattcggg gtatctgctc aacgtcgctt cctcggccgc ttttctaccc
     4201 gggcccttga tgtcgagcta ttacgcttca aaatcctacg ttttcaggct gaccgaagcc
     4261 atctatgaag aactgcgccg gtcaggcagc cgggtatcgg tcagcgtgct ctgccccggc
     4321 ccggtgaaaa ccgagtttga aaaaaccgcc gacgtcaatt tcaccattca gaaactggaa
     4381 agttcccggg tcgcagaata tgccatagac cgcatgctcc ggcgcaagct ggtgattatt
     4441 ccgggggcta ttatgaaaac cgcgcggttt tttgaaagat ttctgcccga aaaagcgctt
     4501 cttcgggccg catggcacat gcaggagcgt aacatgaacg gagattaaat ggaatgaaaa
     4561 aaacttctct tctccgaagt tttttacgct tcggtctgcc cgtctgtctc gtttcctttt
     4621 ccctactttt gatcggcatc cgtttcgcgg cgggaaacca gatgaattcg aaaatcatac
     4681 tcagctgttt catcctgtcg ctgattttcg gcgcggtttc gttcgctttg ttccgtttca
     4741 gaatatggat gctttgtttc tgggcagggc tggccatcgg ctttttcgat atgttccgca
     4801 tcttcattga cggaatggaa ggctggggcg atctgatcgg gctgttctct ctttttatat
     4861 gggcgatcgc gggaatcctg ggcggagccg ccgcccagct cctccgtttt ctcctgcgta
     4921 agttcacagg ccgccgaacc aggcgcgaat aatcttctaa cgtcccacgc tatgctcaaa
     4981 ttcttctttt gcttccttca tcagttccgg ccggctaaaa agctccaaag ccgcaccggc
     5041 aaacgcccgg gccgcaatca gcagcttttg ttttgcctcc cggccgttgg caagcgtgag
     5101 aaacgcggct tcatgaacgt tgaccgatcc gtccccaacg ccgatgtttc catacacagt
     5161 ggggactctg tagctgacat tgccgatatc ggtggagcct gcgttttcaa tctcatcgct
     5221 gaaattttca attcccgctc ttcgcagctg atccttcatc agctcaacca gcacggaatt
     5281 aagcaccaga tcgtcataag aattttcaaa ctgccggatt tccagctttg tcccggtcat
     5341 cagggccgct cccctcgcgc agtttttcac tttttccgaa acgtcgtcaa gacccgctct
     5401 tttttcggaa cggacataaa atttacagct gcaaagatcc ggaatcacat tgggcgcttc
     5461 accgcctttt gtgatgaccc cgtgtattct cacatcctgc ctgagctgct gcctgagcgc
     5521 accgattccc gcaaaagtca ggttaacggc gtcgagagcg ttgataccgt cataaggaaa
     5581 cgcagccgca tggctggctt ttcccttaaa ctcaaattcc cacgaatcga tcgctagcgc
     5641 tttggcgtta aaattattgt tttgataaag gtgcagctgg aaaaccgcgt caacatcgtc
     5701 aaacgctccc ttttcggtca gatccacttt tcttcccgac gtttcttccg caggtgtgcc
     5761 gatcaccacc actttccccc gaaaggcgtc cttcattttg gaaagaatca gggccgctcc
     5821 gattgtgctc gccgcgatcc agttatgccc gcaggcatgc gccgctccgc cctcatagcc
     5881 gggcagcgcg tcatattcgg gaagaaaggc aattttaggg ccctcgccgt caccgaattc
     5941 ggcccggaaa gccgtctcca gacccagata gggagtctca acattaaacc catgatcagc
     6001 gagcaggcct gagagatatt ccgaagaccg gtattccctg tcgccaagtt cgggatgatt
     6061 gaaaatatag tcgctgattt cttcaattgt acctttcatt tcttctgcga tcttatccgc
     6121 cctttgttcc agctgattca tattctgctc ctttaccgtc atcccgattt ctacggacac
     6181 cccgctccgg attcttgccg gagacgggtt ttttatccga ttattctgac tatcattatt
     6241 tcatattttt cgattttatt caattgttac gctttttgaa ttcccgatca acaaaacagg
     6301 ggacggccgg tcggccgtcc cctgtcaatc gtttgttcag attgcaatgg gttcaagact
     6361 gtaaacacag tcggtgtttt tggagacatt gggggaatgg gtaacaatga tgacgcattt
     6421 gttgtcgttg gcggcaagcc ctttgagaat tttcattact tccgcctcgg ttccgccgtc
     6481 gagattgcct gtgggctcgt cggccagaat aatatccgga ttataggaca gcgcccttgc
     6541 aattgccaca cgctgctgct cgcctccgga aagcttcaac acccttctgc ccgccttctc
     6601 agcattgagt ccgacttttt cgagaatgtc gagcgcgtgc tttttcttgt cattgagctt
     6661 cattcccgcc acatccatcg agagcacgac attttccaga gccgtcaggt gaggcagaag
     6721 attgaagctt tgaaaaataa cgccgatgtt ctgactgcgg tatttctcac ggtcgatttt
     6781 cgcaatatca ctgccgtcgt agagaatctt gccgcttttg ctattttcaa gaccggacat
     6841 cagggaaagc agcgttgttt taccggaacc ggacttgccc acaatcgaat aggttttgcc
     6901 tttttcaaac tcaaagttga tatccttcag caccggggtt ttaccgttat catatgaata
     6961 attgaggtgt tctatcgtta agatgctcat ttcaatatcc ctcccgttat gatctgctgc
     7021 tgaggatttt cagcggctcg tagcgtgaaa taaagaccat tgagattccg ctgctgatga
     7081 tggtcagcaa aatgccgatt ccggccatct gcagcaagac cctgccgctg attacagcat
     7141 taatctgatt gacatagctt acgttcttgg atgtgccgaa gaagcctccg atgccggagc
     7201 cgcccatccc tccgccgccg cttcccggtc ggccaaagtt ctgcgcaacc tgcgtctgct
     7261 gggactggat gctgctgatc tggtttttaa gcatgtaatt tgcgaccgga accgatgccg
     7321 cggatcctgc tgcagcgccg atggtaagcg ccgcgaacgt gacaataaag agttctgtta
     7381 caaactgcag cgcgactttg cctttcttca taccgatggc ccggagtacg cccacttcat
     7441 atttgcgttc acggatatta aacatattaa gcacaaccag gataataccg ccgatcgcca
     7501 gcacgagaat caggaagata ccggcaaaat tgctgaggtt tttaagcggt tccaggcttg
     7561 aagtcaccga atcttcattt gtggtaaccg agtaatagct gctcatgcct ttggttttga
     7621 gttccgcttc aaacgagtca acgctgcttg cgctcttgag cgtgaagctt gacgaaagcc
     7681 tggattcaag tttggtggtg gaactgccgg aagccgcggt aacaatctta ttggcctccg
     7741 tataatttgt aaggatcttg ttggccgcgt tcgagaacag attcatctgc gagccgtcgg
     7801 aagacgtcgt atcggtgtag attcccacaa ccttgtacgt cactttctga gtcgcatcat
     7861 tggggttcgt cagaataatc tggctgccga ctttcaagct gtttgcctga gcaagatcgc
     7921 ttgaaattac gcagtcgttc gtcgtatcgc tgtcactgaa catcgatccg gatttgattt
     7981 tataagtacc gcttacaaag tcagtcatcg cggaggtcga actgtagcca accagctgga
     8041 aatcgccctg agactgttcg ctgaaatttc cgccgggagc ctggccgcct tcaacgccgg
     8101 actgctttgc ggcagaggat gagccgctgc ccgttgaact tgtggacgta ttggacgccg
     8161 ccgtgatatt tgtggaatca agcgagaggg atatttgata ttcaagactc ttgacatagc
     8221 tggaatcagc gtatttctta agctgagcca gcgtcaggct gggaacgcta ttcataatgg
     8281 tccgcatatc cgaactgctt gactgcgcct gtttttgaat tgcctccctg ttgacgccga
     8341 ttgttgccgt aatcgtattg ctttcctttt gctgtctgac aacctcgttg gcagagcttc
     8401 gaatggcaag agccacgcag ctggacgccg cgatcaccaa aacaataatg ccaatcagga
     8461 tgtttcttcc ttttgatcgg gagatacttt tccaagcatt tttcactata tacatgcggg
     8521 acactcctta cgctttttat taccatccca tgatagcaag ctattgtgat agatttatga
     8581 cagaaagcgt aagaattcat gaaaacaacg gacaaacaca aataatattc cgctatcgac
     8641 ttgattcaaa accgtaatca taccctgccg cgcgatccat ttgatcgggt gcaggcatgc
     8701 tattatggaa agcgcccgta aaagcgcgct gccgattatg gccacggaaa atcgttgatt
     8761 taatacgcac aacagcaaaa attaagttga caaaccatcc gcaatatgat aatataatca
     8821 agcacaattt aaagaatat
//
LOCUS       DATALY010000376         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_81505,
            whole genome shotgun sequence.
ACCESSION   DATALY010000376 DATALY010000000
VERSION     DATALY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81505"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1657
                     /locus_tag="VHR42_10295"
     CDS             <1..>1657
                     /locus_tag="VHR42_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX3027594.1"
                     /translation="VGAGDAEGSMNAANILKPALSRGDIQVIGATTFKEYRKHIEKDA
                     ALERRFQPVIINEPSMDETFDMLKGIKSFYEEYHKVSIPDEVLRQAVFLADRYINDRF
                     MPDKAIDLLDEACSDLNLKNENLSEIARLEEEVSNLVAQRDIVLQTSGDESIYARIAG
                     LKSRQMKLEAQIGELKAKPLPVLGVDDLSRVIELWTKIPASRVKEQDFERLIHLEERL
                     KTHLIGQDEAVNAVSAAIRRRRAGISEKRKPVSFIFVGPTGVGKTELVKCLAEDLFGT
                     RDALVRLDMSEYMEKHSVSKLIGAPPGYVGYDEAGQLTEKIRRRPYSVVLFDEIEKAH
                     PDVLNMLLQILDDGRITDAQGRYVNFENTIVIMTSNAGSERKDGSVGFGRTVSEQGRE
                     KALKSLKEIMRPEFINRIDEVISFNQLTEEDFSKICIIMLSELKRAMARSGVTMTYDQ
                     SLVEWLVRESYSVKYGARNLRRKIEKEIEDKAAGIIIKKRLQSTVYRRVIYRRSDDQT
                     ICLINLFHPTKFLLRGKCAGISGLAHTTGGAASDFLRPYMEYLY"
BASE COUNT          434 a          400 c          478 g          345 t
ORIGIN      
        1 gtgggagcgg gcgacgcgga aggctcgatg aacgccgcga acattctgaa acccgccttg
       61 tcccgcggtg atattcaggt aatcggagcc accacgttta aagaataccg aaaacatatc
      121 gaaaaggatg ccgcgctgga gagaaggttc cagccggtaa tcatcaacga acccagcatg
      181 gacgagacgt ttgatatgct gaagggtatc aaaagctttt atgaagaata tcacaaggtc
      241 agcattcccg acgaggtgct cagacaggcg gtatttctgg cggaccggta tatcaacgac
      301 cgctttatgc cggacaaagc catcgatttg ctggatgaag cgtgttccga cctgaactta
      361 aaaaacgaaa acctgtcgga gatagcacgc ctggaagagg aggtttcaaa cctggtggct
      421 cagcgggaca ttgttctgca gacatcgggg gacgagagca tttacgcgcg catcgccggg
      481 ctgaaaagcc ggcagatgaa gctggaggct cagatcgggg aactgaaggc gaaaccgctg
      541 cctgttttgg gcgtggacga tttgtcgcgg gtcattgagt tgtggaccaa aatccccgcg
      601 agccgggtga aggagcagga tttcgagagg ctgattcatc tggaggaacg actcaagacc
      661 catttgatcg gtcaggacga ggcggttaat gccgtatcag ccgccattcg ccgtcgcagg
      721 gcgggaatca gcgaaaaacg caagccggta tccttcattt ttgtggggcc gaccggcgtg
      781 ggaaagaccg agcttgtcaa atgcctggcg gaggatctgt tcggcacaag agacgctttg
      841 gtccggttgg atatgtcgga atatatggaa aagcacagcg tttccaagct gatcggcgcg
      901 cctcccgggt atgtggggta tgacgaggcc gggcagctca cagaaaagat tcgacgcaga
      961 ccgtattcgg tggttctgtt tgatgaaatt gaaaaagcgc atcccgatgt tctcaacatg
     1021 ctgctgcaga ttctggatga tgggcggatc accgatgcgc agggccgata tgtgaacttt
     1081 gaaaatacca tcgtcatcat gacctctaac gcgggttcgg agcgcaagga cggttcggtc
     1141 ggcttcggca ggaccgtcag tgaacagggc agggaaaaag cactcaaatc gcttaaggaa
     1201 atcatgcgcc ccgagtttat caaccgtatc gacgaggtca tctcctttaa tcagctgacg
     1261 gaagaagatt tctccaaaat ctgtatcatt atgctcagtg aactgaaacg ggcaatggcc
     1321 cgcagcgggg tgaccatgac ttatgatcaa tcgctggtgg aatggctggt gcgcgagtct
     1381 tattcggtta aatacggcgc acgcaacctg cgccgcaaaa ttgaaaagga aatagaggac
     1441 aaagccgctg gtatcatcat caagaaacga cttcaaagta ccgtttaccg acgcgtaata
     1501 taccggcgat ccgacgatca aaccatctgc ctcataaatc tgtttcatcc aactaagttc
     1561 ctgctcagag gaaaatgcgc agggatttcc ggccttgcac acaccacagg cggtgcagcc
     1621 tcggatttcc tgcgccccta tatggaatac ctctacg
//
LOCUS       DATALY010000377         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_339018,
            whole genome shotgun sequence.
ACCESSION   DATALY010000377 DATALY010000000
VERSION     DATALY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339018"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..698)
                     /locus_tag="VHR42_10300"
     CDS             complement(153..698)
                     /locus_tag="VHR42_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HEX3027595.1"
                     /translation="MNMIIGRRQIILAALVLALGAAIFLNYHFSQTGKSTDLTGLTSK
                     TSNLGDATYVDNQKLSSKATSYFAQARLSRTQSRDQAKETLASVESNSSTSSSQKQEA
                     QEGIKQIATNIQSENAIENIVKAKGFSDCVCFINAGQANVVVATKSTSTLSASDAIQI
                     KDIVMNQSKLSADNIKIIQVN"
     gene            complement(728..1312)
                     /locus_tag="VHR42_10305"
     CDS             complement(728..1312)
                     /locus_tag="VHR42_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027596.1"
                     /translation="MRKSGKMIDFTKIKEMVFSKNGSIILVIIGFIGIGLIFVSQYLP
                     TSTSKASSTSSTSTTDQYAKKTESQLENIIGQISGVGKVKVMVTVGSGVEYVYEQNVK
                     TSTDTTKNNDSSGTTQTQQNSDEETNPVVVENASGGQQALVKTEIQPKVTGVVIVCDG
                     GDDSEVCESIVNTVTTALDLPANHVSVSKRNITK"
     gene            complement(1314..1787)
                     /locus_tag="VHR42_10310"
     CDS             complement(1314..1787)
                     /locus_tag="VHR42_10310"
                     /inference="COORDINATES: protein motif:HMM:NF021121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HEX3027597.1"
                     /translation="MEAVRSWAVAVCLAALAAGMAGVVAPGGNLEKSYKFAVSLFFLC
                     CLLTPLFGLKKIDLNLNLTQTAVSQTYDISDTVKDQEKQVAEDEISQTVRNYCVQQGV
                     TPKSVKTTLIKQKDGKFSVGNVTVIVKQADAKQKNKLISFVKKELGLDIKVNEGG"
     gene            complement(1788..2930)
                     /locus_tag="VHR42_10315"
     CDS             complement(1788..2930)
                     /locus_tag="VHR42_10315"
                     /inference="COORDINATES: protein motif:HMM:NF021088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027598.1"
                     /translation="MKRKLLISLLIFMVMIPMLSQTVMAADTQSILQEQTQKSGASDL
                     YQKAPESAKSSLSQLGIKTPDSASLARFTPANLFRLVCSQAKEAAQKPIKAVLTIFGI
                     LMLCALLNTMKNTFAEKSLKTVFDVVCGLCIAAAIMVPVSQCITQAAQVIEQSSQFML
                     SVLPVFSGLVIASGHPASGALYQGFLVAVSQVISQIAATTFVPMISIYLGFCVIGSVS
                     PGIQINSVAKFFKNIVTWGVVLLLTAFTGILTVQGLISQAADTVTMKTAKFVVGSVVP
                     VIGSTISDAMNTVVGCANLLKTATGAYAIVVFLFMYLPPVINCVLWILAAEISLAFAD
                     LLGINNMSGLLNSVKQALQVLIALILTAALAMIISISVMLLLGMVN"
     gene            complement(3039..3428)
                     /gene="spoIIIAD"
                     /locus_tag="VHR42_10320"
     CDS             complement(3039..3428)
                     /gene="spoIIIAD"
                     /locus_tag="VHR42_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HEX3027599.1"
                     /translation="MNMFQIAGIVITVAALAVLLRQYKQEYSLMLSLGAGILIFMLVI
                     SRAQPVFAQIQSLVSKSGINTGYLEVLMKALGICFITQFAADSCKDAGESAMASKVEM
                     AGKFAVLLVSLPLFAQVAELAVSLMGN"
     gene            complement(3434..3628)
                     /gene="spoIIIAC"
                     /locus_tag="VHR42_10325"
     CDS             complement(3434..3628)
                     /gene="spoIIIAC"
                     /locus_tag="VHR42_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HEX3027600.1"
                     /translation="MDINFIFKIAAIGIIVSVLHQLLMRSGREEQATMTSLAGLVVVL
                     FMIIQQISRLFDTIKSIFRL"
     gene            complement(3715..>4269)
                     /locus_tag="VHR42_10330"
     CDS             complement(3715..>4269)
                     /locus_tag="VHR42_10330"
                     /inference="COORDINATES: protein motif:HMM:NF021090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HEX3027601.1"
                     /translation="AAKKRICVKTEEAEMIKAVGLILMFLVCTAGGAFKSLDLSKRVR
                     QLESFMGAITLISTEIRYFASPTDVIMGKIGALDEYKELRVFGLCRKNLDQYRDFPKA
                     WEQSIEQSKPYLSLNDSDYETLLWFGKVLGTTDVEGETANCERYKNLLGQRLQRARED
                     QRKRGRMYTSLGVLAGVFIVVLLV"
BASE COUNT         1039 a         1127 c          928 g         1175 t
ORIGIN      
        1 gcaacccctt tgatttccct tgctgtcatc cctgctacgg ttgccagtac atcttcagaa
       61 attttaaggg ttcccgcatc tgcgtaatga ttttgttcca tatcacgcgc cctcctttcc
      121 ctctattata ccacatgttg ctaaagacac aactagttta cctgtataat tttaatatta
      181 tcggcagaaa gttttgactg attcatcaca atatctttga tctggatcgc gtcgctcgcc
      241 gatagtgtac tcgtgctttt ggtcgcaacc acaacgttgg cctgaccggc gttgataaag
      301 catacgcagt cgctgaatcc cttggctttc acgatattct cgattgcatt ctcactctgg
      361 atattggttg caatctgctt tattccttct tgcgcttcct gtttctgact gctggatgtt
      421 gaagagtttg attcgacact tgcaagcgtt tcctttgcct gatcgcgaga ctgggtccgt
      481 gagagccttg cctgggcaaa atagcttgta gctttcgaag aaagcttttg attatccaca
      541 taggttgcgt cgccaagatt gctcgtctta ctcgtaagcc cggttagatc cgtgcttttt
      601 cccgtctggg aaaaatggta gttcaaaaaa atggccgctc ccagcgccaa caccagtgcc
      661 gcaaggatga tctgccttct tccaatgatc atattcatgc tgttttcctc ccagtttgtt
      721 tctattttta tttcgttata tttctcttgc tgacactcac atgattcgca ggcaggtcaa
      781 gcgccgtagt caccgtgttg acgatgcttt cgcagacctc cgaatcatcc cctccgtcgc
      841 agacaataac aactcccgtg actttcggct gaatttcggt tttcaccagg gcctgctgac
      901 caccgctggc attctccaca accacgggat tcgtttcctc atcactgttt tgctgagtct
      961 gcgtggtgcc ggagctgtcg ttgttcttgg tggtatcggt acttgttttg acattttgtt
     1021 cataaacgta ttcaacgccg ctgcctacgg ttaccataac ctttactttt ccgactccgc
     1081 tgatttgtcc gataatattt tcaagctgac tctccgtttt ttttgcatat tgatcggttg
     1141 tcgacgtgct tgaagttgaa ctcgctttgc ttgtactggt cggcagatac tgtgacacga
     1201 atatcaggcc gattccaata aaaccgatga taacaaggat tatggatccg ttcttgctga
     1261 aaaccatctc tttgattttg gtaaaatcaa tcattttgcc tgatttcctc atgttatcca
     1321 ccttcattta ccttaatatc cagccccaac tcttttttca cgaatgagat caatttgttc
     1381 ttctgcttag catccgcctg ttttacaatg accgtgacgt ttcccacaga aaacttgccg
     1441 tctttctgtt tgatcagcgt cgtttttacc gatttcggcg tcaccccctg ttgaacgcag
     1501 taatttcgga ccgtctgcga aatttcatct tcagccacct gtttttcctg gtctttcacc
     1561 gtatcggaaa tatcgtaagt ttgagaaaca gccgtttgag tcagattcaa attaagatca
     1621 atctttttta atccgaataa cggcgtgaga aggcagcaga ggaaaaaaag cgaaactgca
     1681 aacttgtatg atttttcaag atttccgccc ggggcgacca ctccggccat tccggcggcc
     1741 agcgcggcca gacagacggc aaccgcccac gatcgaaccg cttccattta attcaccatc
     1801 cccagaagaa gcatgacaga tattgagata atcattgcca gtgccgctgt caggatcagc
     1861 gcgatcaata cctgcaacgc ctgcttcaca gaatttaaaa gacccgacat attattgatc
     1921 ccgagcaggt cggcgaacgc aagggaaatt tccgccgcca gaatccacag gacacaatta
     1981 atcaccggcg gaagatacat gaaaagaaac actactattg catatgcgcc agttgccgtt
     2041 tttagaagat ttgcgcaccc aacaactgta ttcattgcgt cgctgattgt gctcccgatg
     2101 accgggacta cgcttcccac cacaaacttg gctgttttca ttgttacggt gtccgccgcc
     2161 tgtgagatca gcccctgaac cgtcaggatg ccggtaaaag cggtcagtaa cagcacaacg
     2221 ccccaggtaa cgatattttt aaaaaattta gcgacactgt tgatctgaat tccgggagat
     2281 accgagccga ttacgcagaa cccaagataa atgctgatca ttggcacaaa ggttgtggcg
     2341 gcaatttgag aaatcacttg ggataccgca accagaaatc cctgataaag cgctcccgaa
     2401 gcgggatggc cggaggcaat gacaagtccg ctgaataccg gcagcacgga gagcataaat
     2461 tgcgacgact gctcaatgac ctgcgccgcc tgcgtaatgc attgcgaaac cggaaccata
     2521 atggccgccg cgatacaaag gccgcagacc acgtcaaata ccgttttcag ggatttttcg
     2581 gcaaaggtgt ttttcatcgt gttcagcagc gcacagagca tcaggatacc aaaaatggtt
     2641 aataccgctt taattggttt ctgcgcggcc tctttcgcct gactgcagac cagccgaaac
     2701 agattcgccg gtgtaaaccg cgcgaggctt gcggaatccg gtgttttgat tccaagctgg
     2761 ctaagtgatg attttgcgct ttccggcgct ttctgataga gatccgacgc accgcttttc
     2821 tgggtctgct cctgaagaat gctttgcgta tccgccgcca tgacagtctg agacagcatt
     2881 gggatcatca ccataaaaat aagtaagctg atcaatagtt ttcttttcat tccgttatcc
     2941 gcctttttac tgcctgaacc attcaaattc atttgtctta gggctgatcc tgaaagaaac
     3001 aatcgcgtag aattattttg aaatatatat cttccttatc agttgcccat caaactgacg
     3061 gcgagctcgg caacctgcgc gaaaagcggc aaagagacca gcagcaccgc gaatttcccc
     3121 gccatttcca ctttggaagc catcgcactt tcccccgcat ccttgcagga gtcggcggca
     3181 aactgcgtga taaagcaaat tcccagagct ttcatcagca cttccagata tccggtattg
     3241 ataccggatt tggataccag cgactgaatc tgggcaaata ccggctgggc tctgctgatc
     3301 actaacataa aaataagtat cccggcgccc agactgagca tcagggaata ttcctgctta
     3361 tactgcctga gcagaacggc caacgccgcg accgtaatca caattcctgc aatttgaaac
     3421 atattcatct ttatcacagc ctgaatatag attttatcgt atcaaacaat ctgctgatct
     3481 gctggatgat cataaacagt acgacaacca ggccggcaag ggaggtcatc gtggcctgct
     3541 cctcgcggcc tgaccgcatc agcagctgat gaagcaccga cacgatgatt ccgatggcag
     3601 cgattttaaa aatgaaattg atatccattt caattcctcc atgccgcggg ggcatctgat
     3661 tttttcatac cggccggagc ggtgacgccg gtcaggcctt tcggtccata tccgtcagac
     3721 caaaagaacc acaatgaaaa cgccggccag cacacccagg gaagtataca ttctcccccg
     3781 tttccgctga tcttcccgcg cgcgctggag tctctgcccc agcaggtttt tatatcgttc
     3841 acaattcgcc gtttcccctt cgacatcggt agttccgagc acttttccaa accagagcag
     3901 cgtttcataa tcgctgtcgt tgagcgaaag atacggtttg gattgttcaa tcgattgctc
     3961 ccacgctttg ggaaaatccc tgtattgatc gagatttttc ctgcacaggc caaaaacgcg
     4021 aagctcttta tattcgtcga gtgcgccgat ttttcccata atgacgtcgg tcggggaagc
     4081 aaaatagcgg atttcggtcg agatcagggt aatcgccccc ataaatgact caagctgccg
     4141 gacccgtttg gagagatcga gcgacttgaa ggcccctccg gccgtgcaga ccagaaacat
     4201 caaaattagt cccaccgctt tgatcatttc agcttcctcc gttttgacac agattcgttt
     4261 tttcgctgc
//
LOCUS       DATALY010000378         8346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_687049,
            whole genome shotgun sequence.
ACCESSION   DATALY010000378 DATALY010000000
VERSION     DATALY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687049"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..531
                     /locus_tag="VHR42_10335"
     CDS             46..531
                     /locus_tag="VHR42_10335"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX3027602.1"
                     /translation="MENEFITFTIGKQKTIALIAHDGKKRELIEWCSENQEILRNHFL
                     CGTGTTANLVAEKTGLPVKGYNSGPLGGDQQIGAKIVEGVINFVVFFSDPLEAQPHDP
                     DVKALLRIAQVYDIPIANNRATADFMIHSEFMNREYEHKVINFRQNVIDRANDKSLLN
                     K"
     gene            548..775
                     /locus_tag="VHR42_10340"
     CDS             548..775
                     /locus_tag="VHR42_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX3027603.1"
                     /translation="MITVKVRLDSVEKVKEFSSVISRVEAECELIEGVHILDAKSIMG
                     IFSLNLAEPIQLDIHSDDRKILDRLKNFIVA"
     gene            917..1582
                     /locus_tag="VHR42_10345"
     CDS             917..1582
                     /locus_tag="VHR42_10345"
                     /inference="COORDINATES: protein motif:HMM:NF044761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3027604.1"
                     /translation="MKSPETRKEELLAAATALFRKDGVDRTAVSDIVREAHVSQGTFY
                     NYFQSKDEIFAAVLEQATSDMMEEMRKTGERTDLDPFQKLKLLSLQDFQLNRTNDSLF
                     DVLHESRYADAHQKYIVARIEKLRPIYANVIRQGVEMGVFNTPYPEEAAYFMLTAIKF
                     VFDPAFFTYSEEGMLRMGQAVGDFCERILGAAHDADSSGGDEWANSIKKYLGRNQHES
                     SFS"
     gene            1563..2798
                     /locus_tag="VHR42_10350"
     CDS             1563..2798
                     /locus_tag="VHR42_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3027605.1"
                     /translation="MKVVFHDEQMSFQTLRLLSEIPSGLADVNEVIGTARKITEGSYE
                     SWCEEWTKTADRVRTQADQFLAGGHRLSAAETFLRACNYYRTAGFYRGLLIADGCTEE
                     VRENLKKLDGLSLECFSKVIQYGKTKIEPIEVPYENTTLPGHFYRMPSEEDFKTAPTL
                     VMINGYDGTKEEMYGCALEALKRGMNCFAFEGPGQGEMIRTRGIPFRADWENVVTPVL
                     DYLIQELGVAPDRIVLWGESMGGYLAPRAAAFDHRAAACVANSGVYDFMGTRGPQGID
                     RNTYFRSIEQMPDAAFEAIIKKQMDSVIQLNWALKHGMYVFQAKSLKDYFLKAKDYYM
                     GDIAGQIACPTLVIDTEGENNFPGQAKMLYDALNCPKDYLLFKEAEGAEEHCEEGAKL
                     YANCRIFDWIEDTLGGLGR"
     gene            complement(2887..3657)
                     /locus_tag="VHR42_10355"
     CDS             complement(2887..3657)
                     /locus_tag="VHR42_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3027606.1"
                     /translation="MEYTVKKLALLSGVSPRALRFYDEIGLLRPARINSSGYRLYGEQ
                     EVNLLQQILLYRELGLSLEEIKQIVTQPDFDVLAALQEHRKQLLEQRTRLDALIENVE
                     KTIRNAKGETSMTDSEKFEGFKQKLIEENEQKYGAEIREKYGVETVEQANRKLAGMTE
                     EEFSRAAALEKQMIELFLEAFPTGDPAGEIAQRAADLHRQWISFYWAQYSPEAHAGVA
                     RMYVADPRFTAYYDKYQPGLAKFLRDAVLIYTGQPKNQ"
     gene            3858..4319
                     /locus_tag="VHR42_10360"
     CDS             3858..4319
                     /locus_tag="VHR42_10360"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX3027607.1"
                     /translation="MNFLSDDIRVLRFNGVLINGYSRKQVDEVLRQIAEDYREREDTI
                     EDLKNKISMLKETVDHYKTIEESMQHCLIIAQHTGDEIVKSANEKAKDILDDADSTSH
                     RLISEAYQQANKIKLSFDDLKAKVSTYRMKSEAILKSQLDVLNQLPDDSAE"
     gene            complement(4392..5336)
                     /locus_tag="VHR42_10365"
     CDS             complement(4392..5336)
                     /locus_tag="VHR42_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX3027608.1"
                     /translation="METDSRIYVAGHTGMVGSALVRGLKRQGYTNLVARTHAELDLTD
                     QAATERFFREEKPEYVFLAAARVGGICANSTYPADFIMENMMIECNVIRSACARGVKK
                     LMLLGSSCIYPKLCPQPIKEEYLLTGELEPTNEAYALAKISGIKMCQSYNMQYGTKYI
                     SVMPASLYGLNDRFDFNNSHVIPAMMMKFYRAKLEERPFVELWGTGTPLREFLYTDDM
                     ADACISLMNHYEGNDFINIGSGEEISIRELSEIIRKTVGYTGEIIFDPSKPDGTPRRV
                     LDNSKILQTGWRPQVPIEEGIRREYEYFLEKIMPDLFS"
     gene            complement(5370..6401)
                     /gene="gmd"
                     /locus_tag="VHR42_10370"
     CDS             complement(5370..6401)
                     /gene="gmd"
                     /locus_tag="VHR42_10370"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX3027609.1"
                     /translation="MKKALITGITGQDGSYLAEFLLHKGYEVHGLVRRSSSANTQRID
                     HLLEEDNESGSRLFLHYGDLNDTGSISRLVDRTAPDEIYNLGAQSDVKISFDVPEFTS
                     DVDALGALRLLESINDINPLIRYYQASSSELFGRVREIPQNENTPFYPRSPYAAAKLY
                     AYWITVNYREAYHLFASNGILFNHESPRRGENFVTRKITKGIAKILKGEQEKLMLGNR
                     DAKRDWGFAGDYVEAMWMILQQQEPDDYVISTGETHTVREFCELAFRCAGMELEWVGK
                     GIEEKGIDRETGRELVAVSSRYFRPSEVNLLLGDSSKAREKLKWAPKVSFEQLTAMMV
                     ESDRKAAGV"
     gene            complement(6531..7430)
                     /locus_tag="VHR42_10375"
     CDS             complement(6531..7430)
                     /locus_tag="VHR42_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3027610.1"
                     /translation="MEQTQKMYMDYIAAHFPGTDPGQIQFDFFSDPYSDIVSFGGRVL
                     KFSRYDWSAAHLMNEVGAIRLIRPYVDLPLPHVNLLETDVSLCEPVPGKPLLRHRLFA
                     LKNTEQSLIAAQLGVFLKQLRSIPQEEALRHGIRETSPSLEPGFWKREYDDLWRKIAP
                     YCDNSAKEQIQELFGHVWDNEKFLQFTPSVLHGDLTPEHIRYDCGTNGICTVTGFGLA
                     RLGDPAEDYAALLAGYGEGFLRRMEKHDDALPELIDRARFYASLRPLRRARELADRIT
                     TRDFSGFRLCLTESDLMPVGFPW"
     gene            complement(7500..>8346)
                     /locus_tag="VHR42_10380"
     CDS             complement(7500..>8346)
                     /locus_tag="VHR42_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-fucosyltransferase family protein"
                     /protein_id="HEX3027611.1"
                     /translation="VYWGIESMYSLSMNTNSFESFFEPVSDCSLEEVIRPEFSYYPPS
                     WNFQNALADDTDKLRMDHRDLKGMMESNADVLISDIYYPISAMLPWVGAGHWAYGKTP
                     VQIYRTLYDKYLKLKPETKREIQKYVNTTPNFRDERPIIGVHVRENAIVNEVAQLYDL
                     NEFYKPRIWDFIVRFNARHIFLITDSRKLFREYKKLYSRNGLLMYTDSKKELFQPRVM
                     TCLLDYPNKRHKGAELQKDTVEIIKDTYLAAQCDFFIGNGYSSLSNAVMHLKDWPEPN
                     IKLLY"
BASE COUNT         1998 a         2063 c         2221 g         2064 t
ORIGIN      
        1 tccagtcggc gtggaaagaa cataaaataa aatcaggaga acaatatgga aaatgaattc
       61 ataacattta caatcgggaa gcagaagacg atcgccctga ttgcccacga cggaaaaaag
      121 cgggaactga ttgagtggtg ctcggagaat caggaaattc tgcgcaacca tttcctctgc
      181 gggacgggca cgacggcaaa tctggtggcg gaaaaaaccg gtcttccggt aaaaggctac
      241 aacagcggcc ctctgggagg cgaccagcag atcggtgcga aaatcgtgga gggcgtcatc
      301 aactttgtgg ttttcttttc cgatcctctg gaagcgcagc ctcatgatcc ggacgtgaaa
      361 gccctgttac gtatcgctca ggtttacgat attcccattg cgaacaaccg ggcgaccgcc
      421 gatttcatga ttcattccga attcatgaat cgggaatatg aacataaagt aatcaatttc
      481 aggcaaaatg tgatcgaccg ggcaaacgac aaaagcctgc tgaacaaatg agaaagcgcg
      541 gtggattatg ataacggtta aagtacggct tgacagtgtt gaaaaagtaa aggaattctc
      601 ctctgtgatt tccagagtgg aagcggaatg tgagctcatc gaaggggtcc atattctcga
      661 tgcaaaatcc atcatgggaa ttttcagcct gaatctggcg gagccgattc agcttgacat
      721 tcattccgac gaccgcaaga ttctggaccg tctgaagaat ttcattgttg cgtaacaggc
      781 atcgcctcgt ttcgcgcccg gggaaaatcc cgattcgcac atttttggtg cggattggga
      841 ttttcttatt gacaaataga aaaaagagac tatactgttt gataatgact gacgtcagtc
      901 agaaaggaaa gaacaaatga aatcacccga aaccagaaaa gaggaactgc ttgcggcggc
      961 aaccgcacta tttcgaaaag acggagtgga ccgcacggcg gtcagtgata ttgtgcggga
     1021 agcacatgtc tcgcagggga cgttttacaa ttactttcag tccaaggatg agatattcgc
     1081 cgccgttctg gaacaggcga cgtcggatat gatggaagaa atgcggaaaa cgggagaaag
     1141 aaccgatctt gacccctttc aaaagctgaa attactttcc ctgcaggatt ttcagttgaa
     1201 ccgcaccaac gacagcctgt tcgacgttct gcatgagagc cgctatgcgg atgcccatca
     1261 gaaatatatc gttgcccgca ttgagaagct tcggcccatc tatgcgaacg tgatccggca
     1321 aggcgtggag atgggtgtat tcaacacgcc ctatccggaa gaggcggcgt attttatgct
     1381 cacggcgatc aaatttgtat ttgatcccgc cttttttacc tattccgagg aaggaatgct
     1441 ccggatgggg caggccgtcg gagatttctg tgagcggatt cttggtgccg cgcatgatgc
     1501 agacagcagt ggcggggacg agtgggcgaa cagtatcaaa aaatatttag ggaggaatca
     1561 gcatgaaagt agtttttcat gatgagcaga tgtcttttca gacactgcgc ctgttgagtg
     1621 aaatcccttc ggggctggcg gatgtaaatg aagtaattgg cacggctcgg aagattaccg
     1681 agggcagcta tgaaagctgg tgcgaagagt ggacgaaaac cgccgatcgg gtccggacac
     1741 aggccgatca gtttttggcc ggcggtcacc gtctgagcgc ggcggaaact ttcctgcgcg
     1801 cctgcaacta ttaccgtact gccggattct accgcggtct tctcatagcc gacggctgta
     1861 ccgaagaagt cagggaaaac ctgaaaaagc tggatggtct gtctctggaa tgcttttcca
     1921 aagtgattca gtacgggaaa acgaagattg aacccatcga agtgccgtat gaaaacacaa
     1981 ctctcccggg tcatttttac cggatgcctt cggaggagga tttcaaaacc gccccgaccc
     2041 tggtcatgat caacggctat gacggcacaa aagaagaaat gtacggctgt gcgctggaag
     2101 cactcaagcg cggcatgaac tgttttgcgt tcgagggacc gggacagggc gagatgattc
     2161 gtaccagagg gattccgttc cgcgcggatt gggaaaacgt ggtgacgccg gtgctggatt
     2221 acctgattca ggagttggga gttgccccgg accggatcgt tctgtgggga gaaagtatgg
     2281 gcggctatct cgcgccgcgg gccgccgcct tcgatcaccg tgccgccgcc tgtgtcgcca
     2341 acagcggggt ctatgatttt atggggacgc gcggaccgca ggggatcgac cgaaatacct
     2401 atttccgctc gattgaacaa atgccggatg ctgcatttga agcaattata aaaaagcaga
     2461 tggattcggt gatccagctg aactgggctc taaagcacgg catgtatgtt tttcaggcga
     2521 aaagtctgaa ggattacttc ctgaaggcaa aggattatta tatgggcgac attgccggac
     2581 agattgcctg tccgacgctc gtcatcgaca ccgaggggga aaacaatttt cccggtcagg
     2641 cgaaaatgct gtatgacgct ctgaactgtc cgaaggatta tctgcttttc aaggaagcag
     2701 agggcgccga ggaacattgt gaggaaggcg ctaagctgta tgccaactgc cggattttcg
     2761 actggatcga ggatacgctg ggcggactcg gacggtagat actgacataa ggaccacgct
     2821 ttacagagaa ttggaaagcc cgccgggata cctctttcgt atcccggcgg gcttctctgc
     2881 tttatgctat tggttcttcg gctgcccagt gtaaattaaa acggcgtccc gtaaaaattt
     2941 cgcaaggccc ggctggtatt tgtcgtaata ggccgtaaag cgcgggtcgg caacatacat
     3001 tcgggcaacg cccgcatgtg cctccgggct gtactgagcc caatagaacg aaatccactg
     3061 ccggtgaagg tcagccgcgc gttgcgctat ttctcctgcc ggatcgcctg ttggaaaagc
     3121 ttccagaaag agttcaatca tctgcttttc cagcgccgcc gcccggctga actcctcttc
     3181 cgtcataccg gccagtttcc ggtttgcctg ttccaccgtt tccacgccgt atttttcgcg
     3241 gatttccgct ccatattttt gctcattttc ttcgatcagc ttttgcttga agccctcgaa
     3301 tttttctgaa tcggtcatcg atgtttctcc tttcgcgttt cggatagttt tttccacgtt
     3361 ttcaatgagc gcgtccagcc ttgtccgctg ttccagaagt tgcttgcggt gttcttgcag
     3421 tgctgccagc acgtcgaaat ccggctgggt gacaatctgt ttgatttcct caaggctgag
     3481 accaagctcc cggtagagca gaatctgctg cagcagattc acttcctgtt cgccgtacag
     3541 ccgatagccg gaagaattga tccttgccgg acgcagcagt ccgatttcat cgtagaatct
     3601 cagggcacgg ggactcaccc cggaaagcag tgccagcttt tttacggtgt attccatgca
     3661 atcgacctca tttcctcttt tgtgaatccg gttccactga aaagaatcat acacctttcc
     3721 gctacgtcaa ggtcaatacc tgttttaact attttttgtt tcggttggcg gagcgaaaaa
     3781 aggattcacc ttgcggtaat tgcagaatat tataatacag aataatattt atcggaaacg
     3841 gcaggagggc gaggcaagtg aactttcttt cggatgacat tcgggtgctt cggttcaacg
     3901 gtgttctaat caacggttac agcagaaaac aggtggatga ggtgcttcgg caaatcgccg
     3961 aagactaccg ggaaagggaa gatacaatcg aagatttgaa aaacaagatt tccatgttga
     4021 aggaaacagt cgatcactat aagacgattg aggaatccat gcagcactgc cttattattg
     4081 cccagcatac cggcgatgaa atcgtcaaaa gtgcgaacga aaaggcgaag gatattctcg
     4141 acgacgcaga cagtacttct cacaggctga tcagcgaagc ctatcagcag gcgaacaaga
     4201 ttaagctttc ctttgacgac ctgaaagcga aagtcagtac ctatcggatg aagtcggaag
     4261 cgattttgaa atcgcagctg gatgttctga accagcttcc ggatgattcc gccgaatagt
     4321 attttgccgg agagagaaaa aagagcggct cctgcatcgg caaacagccg gtacagaagc
     4381 cgcttttcca atcaggagaa aaggtcgggc atgatttttt ccaaaaaata ttcgtattcc
     4441 cgccgaatcc cttcctcaat cggtacctga gggcgccatc cggtttgcag aattttgctg
     4501 ttatccagca ctctccgggg ggttccgtcc ggcttggagg ggtcgaagat gatttcaccg
     4561 gtatacccga cggttttccg aatgatttcc gaaagttccc gaatactgat ttcctcccca
     4621 gaaccgatat tgataaaatc gttgccttca tagtgattca tcagactgat gcaggcgtcc
     4681 gccatgtcat ccgtgtaaag aaattcccga agcggggttc cagtgcccca cagctcgaca
     4741 aacggccgct cttccagctt ggcacggtaa aatttcatca tcatggcggg aatcacatgg
     4801 gaattgttaa agtcgaaccg gtcgttcagt ccgtatagac tggccggcat gacggagata
     4861 tacttagtgc catactgcat gttgtacgac tgacacattt tgatgccgga tattttcgcg
     4921 agcgcatacg cctcgttggt gggttccagc tcgccggtca gaagatattc ctccttgatc
     4981 ggctgcgggc aaagctttgg atagatgcag gagcttccga gcagcatcag ctttttgacc
     5041 ccgcgggcgc aggcacttcg gatcacgttg cattcaatca tcatattttc cataataaaa
     5101 tcggcgggat aggtgctgtt tgcgcaaatt cccccgaccc tggctgctgc aagaaagaca
     5161 tattccggtt tttcttcccg gaaaaaacgt tccgtggcgg cctggtcagt caggtcaagc
     5221 tcggcgtggg tccgcgcgac aaggttggta tagccctggc gctttaggcc tcttaccagt
     5281 gcggagccaa ccataccggt gtggcccgcc acatagattc tgctgtctgt ttccaaagat
     5341 tatgcccccc gttttctgaa attgccggat cagaccccgg cggcttttcg gtcactttcc
     5401 accatcatgg cggtcagctg ctcaaacgat actttcggcg cccatttcag cttttcccgt
     5461 gctttggaag aatctccgag cagcaggttg acttcgctcg gacggaaata ccggctgctg
     5521 acggcgacca gttcacgacc ggtttcccgg tcgattccct tttcctcaat gccttttccg
     5581 acccattcca gttccatgcc tgcacagcgg aacgcaagct cgcagaattc gcggaccgtg
     5641 tgggtttcac cggtcgagat aacataatcg tccggctcct gctgctgcag aatcatccac
     5701 atcgcttcca cataatcccc ggcgaagccc cagtcgcgct tggcatcccg gttgccgagc
     5761 atcagctttt cctgttcccc tttcagaatt ttggcgattc ccttcgtgat tttccgtgtg
     5821 acaaaatttt cgccccgccg aggtgattca tggttaaata ggattccgtt gctcgcgaac
     5881 aggtgatagg cttcgcggta attcacggtg atccaatagg cgtacagctt tgcggccgca
     5941 taagggctgc gcgggtaaaa cggggtattt tcgttctgcg gaatctctct tactctgccg
     6001 aacagctcgc tggaggaggc ctggtaatac cggatcagcg ggttgatatc gtttatggat
     6061 tccaacagcc gcaatgcgcc gagggcgtcg acgtccgagg tgaattcggg aacgtcaaaa
     6121 gagattttta cgtcactctg cgcgcctaga ttatagattt cgtccggcgc cgtccggtca
     6181 accaaacggc tgatgctgcc ggtgtcgttc aggtctccgt aatgcaggaa aaggcgactg
     6241 ccgctttcat tgtcttcctc cagtaaatga tcgatcctct gtgtgttggc ggaactgctt
     6301 ctgcgcacca gtccgtgaac ttcatatcct ttatgaagca gaaattctgc cagataagaa
     6361 ccatcctgtc ccgttatgcc tgtgatcaat gcctttttca tagttacctc ttatgcttct
     6421 atataaatta aaaatcaaga agtccggctt ctgatttatt aatattctat gaaatcgagc
     6481 gtggtcctgt gatttgcgcg attctgcgtg ggaatgaaaa acaaaacctt ttaccatggg
     6541 aacccaaccg gcataagatc ggactccgtc aggcagaggc ggaatccgga aaagtcgcgc
     6601 gttgtaatcc ggtcggccag ttcccgcgcg cgtcttagcg gccgcaggct ggcgtagaag
     6661 cgtgcccggt caatcagctc cgggagtgcg tcgtcgtgtt tttccatgcg ccgcaggaag
     6721 ccttcgccgt acccggccag cagagcggcg taatcctccg ccgggtcacc aaggcgggca
     6781 aggccgaagc ctgttacggt gcagattccg tttgtgccgc agtcatagcg gatatgctct
     6841 ggggtcaggt ctccgtgcag gaccgagggg gtgaactgaa gaaacttttc gttatcccat
     6901 acgtgcccga acagttcctg aatctgttct ttcgcggaat tgtcgcaata aggggcaatt
     6961 ttccgccaca ggtcatcata ttcccttttc cagaaaccgg gttccagcga aggggacgtt
     7021 tcccgaatgc cgtgccggag cgcttcctcc tgcgggatcg atcggagttg tttgaggaaa
     7081 acaccgagct gggcggcaat caggctctgt tcggtatttt tgagggcaaa cagccgatgc
     7141 cgcaggagcg gctttcccgg tacgggttcg cagagggaaa cgtcggtttc caaaaggttc
     7201 acatggggca gggggaggtc gacgtaagga cgaatcagcc ggattgcccc gacttcgttc
     7261 atcagatggg cggccgacca gtcataacgg gaaaatttca gaactctgcc gccaaaggaa
     7321 acgatatcgc tgtaagggtc gctgaaaaag tcaaattgaa tctgtcccgg atcagttccg
     7381 ggaaaatggg ctgcgatata atccatatac attttttgcg tttgttccat agtggtattt
     7441 ctcctccgtg accggcgccg gaagaattca gacgcctggg aaaaaccgaa tcgctttttt
     7501 cagtacagca gtttaatgtt tggttccggc cagtctttca ggtgcatgac ggcgttggaa
     7561 aggctggaat atccgttgcc gatgaaaaag tcgcactgag cggcaaggta ggtgtccttg
     7621 ataatttcaa cggtatcctt ttgcagttcc gcgcctttgt gccgtttgtt gggataatcc
     7681 agaagacagg tcatcacccg cggctgaaac aactcttttt tgctgtccgt atacatcagc
     7741 agcccgtttc tgctgtaaag ttttttgtac tcccggaaca attttcggga atcggtaatc
     7801 agaaagatat ggcgcgcgtt gaaccggacg atgaaatccc agatgcgcgg tttataaaac
     7861 tcgttcaggt cgtatagctg tgccacttca tttacaatcg cattttcacg cacgtgaacc
     7921 ccgatgatcg gccgttcgtc ccggaaattc ggagttgtgt tgacgtattt ttgaatttcc
     7981 cgtttggttt ccggtttcag tttcaggtat ttatcataca gagtccggta aatctgaacc
     8041 ggtgttttcc catacgccca gtgcccggct ccgacccacg ggagcattgc gctgatcggg
     8101 taataaatgt cgctgattaa aacgtccgcg ttgctttcca tcattccttt caggtcacgg
     8161 tgatccatgc gcagcttatc ggtgtcgtcc gccagagcat tctggaaatt ccatgaggga
     8221 gggtaatagc tgaattccgg ccggatcacc tcttcgaggg agcagtcgga aaccggttcg
     8281 aaaaagcttt caaaggaatt ggtgttcatg gaaagactgt acatgctttc gatgccccag
     8341 taaacg
//
LOCUS       DATALY010000379         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_687318,
            whole genome shotgun sequence.
ACCESSION   DATALY010000379 DATALY010000000
VERSION     DATALY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687318"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2827)
                     /gene="addA"
                     /locus_tag="VHR42_10385"
     CDS             complement(<1..2827)
                     /gene="addA"
                     /locus_tag="VHR42_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEX3027612.1"
                     /translation="MAERVWTKDQNDAITAPGTLLVSAAAGSGKTAVLVERVIQKITD
                     SENPCDADRLLVVTFTNAAASEMRERLSQRLEQMIAENPEDLNLQRQQILLQNSHIST
                     VHAFCLDLIRENFEKLDLSPDFRIADDSEMKVLLQDTLDQLLEENYADAVRDSSVDNI
                     DEKPFIILSEMLGTGRDDTQLADAVIRLHRFVRSLPDPQGWLSEKLEMYRPGRKITET
                     EWGKPALDYLSSAIGNDIALTEQALSEIHRDERLIKAYGPAFESDLSGLRSILRLTES
                     GDWDGVCRAAGEFGFQKLGPLRKFEDERLKSRVTGLRDAVKKSLVSLKKGILCCDEQE
                     FQSDINQLYPVVRCLFEIVGHLDQRLFEEKIGRNILDFSDLEQLTLRLLVEKKAGGFV
                     PTSIAERVSRRFEEVMIDEYQDTNLAQDTIFRSVSRGETNLFMVGDVKQSIYRFRQAM
                     PEIFIEKKERFAPYGEGVSPARIILGKNFRSRSGVTDAVNFIFHLLMTKQMGEISYGR
                     EEELIPGADYPVRQEPDFEIRVVDASGYEGEDDRDTLEAQAVASMIRKMVDSGFTVSA
                     RGGTRPAEYRDFCVLMRSTSGRAEKYLNALAGAGIPAYSDLSNGYLGSYEVSVILSLM
                     RILDNPLQDVPLLSVMFSPAFGFEPDDLAKIKLENRKKSLYLALTRYASVHGGKYQDF
                     LSMIGKLRSLAAVLPTDRLLLRIYSMTGFPNICGAMTNGEKRRANLRLLLDYARNYEA
                     AGYRGLSGFIRFIDRLGEQNGDLAPASTVTESANVVRVMSIHKSKGLEFPVCVLAGCA
                     RQFNREDLNKAALFHPVYGFGSMVRDAKLNCRYTTLPREIVRLETGRSALSEELRTLY
                     VALTRAKEKLIAVLTPANPEGTLKKAAALIAPNGKDGALISPYTAGRAQSYAEWLLAC
                     ALLHPSGSSLRERAGISSYPTAPAGRDW"
     gene            complement(2820..>4095)
                     /locus_tag="VHR42_10390"
     CDS             complement(2820..>4095)
                     /locus_tag="VHR42_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX3027613.1"
                     /translation="MTRAIARFFSEKPEYERRMAVLKRAADKTPFTFRDVTNARALFG
                     ERMQISPSRLETFRQCRFLYYCRYGLRAFPRGRAEMDAPEIGTLIHFVLERLLREHSG
                     EGLWTIARDELSSQVKSLLKEFADGFLGGLKDKPERFKYLYLRLEGQVIGLIEHIARE
                     LEQSMFRPVDFELTIGRDVKPLTVDLPDGGSITVEGKVDRVDLMKRDGASYLRVVDYK
                     TGSKEFNLSDVIYGLNLQMFLYLFALCDNGGGRYSAQGEITPAGVLYVPSGRPNITAA
                     RSAGEEDISAARDSAYKMSGLLLDDPEVIRAMERDGSGVFIPAKLRKSEDADGNVSYE
                     PDSRASVASLEKFGFLKRHVVKTLKDMALTLHEGDVAAEPVQGISYHPCDYCDYACVC
                     GHEPGDGIRLLRKYDKEEVWKSLKEEGGVENG"
BASE COUNT          922 a         1205 c         1046 g          922 t
ORIGIN      
        1 cccagtcccg ccctgccggg gccgtcggat aggaggaaat cccggcccgc tcccgcagag
       61 acgaaccgga cggatggagg agcgcgcagg cgagcagcca ttccgcatat gactgcgccc
      121 tcccggcagt atagggcgaa atcagcgcgc cgtctttgcc gtttggggca atcagggcgg
      181 cggccttttt gagcgtgccc tccgggttgg ccggggtcag aacggcgatc agcttttctt
      241 tggcccttgt gagagccaca tacagtgtcc tgagttcctc cgacaaggcg ctgcggccgg
      301 tttcaagcct gacgatttcg cgcggcagcg tggtgtagcg gcagttgagt tttgcatccc
      361 ttaccatgga gccgaaaccg taaaccggat ggaacagggc cgccttgttg agatcctccc
      421 ggttaaactg cctcgcacag ccggcgagca cgcagacggg aaactccagt ccttttgatt
      481 tatggatgct catgacccgc accacattcg ccgactcggt tacggtggaa gctggggcaa
      541 ggtcgccgtt ctgctcgccg agcctgtcga taaagcggat aaaaccggaa aggccgcggt
      601 agccggccgc ctcgtaattt ctggcgtaat caagcagcag ccttaaattg gcccgccgtt
      661 tttccccatt ggtcattgcc ccgcagatat tgggaaatcc ggtcatgctg taaatgcgga
      721 gcaggagtcg gtcggtcgga agaacagcgg caagacttct caattttccg atcatggaaa
      781 ggaaatcctg atatttgccc ccgtgaaccg atgcgtatcg ggtgagagcc aaatacaggc
      841 tctttttacg gttttccagt ttgattttgg cgagatcatc cggctcaaag ccgaatgcgg
      901 gtgaaaacat caccgacagc agcggaacat cctgaagcgg attatccaaa attctcatca
      961 gcgacaggat caccgagacc tcataagagc cgagataacc gttcgacaga tccgaataag
     1021 cgggaattcc cgcgccggca agcgcgttga ggtatttttc cgcccggccg cttgtgctgc
     1081 gcatcagtac gcaaaaatcc cggtattccg cgggccttgt gccgccgcgg gcgctgacag
     1141 tgaaaccgga atcgaccatt ttccgtatca tcgaagcgac ggcctgtgct tcaagcgtat
     1201 cgcggtcatc ctccccctcg tagccggaag cgtcaacgac gcggatttca aagtcgggtt
     1261 cctgccttac gggataatcc gcgcccggaa tcagctcctc ctcccgaccg taggagattt
     1321 cacccatttg tttggtcatc agcagatgaa aaatgaaatt gaccgcgtcg gttacgccgc
     1381 tccggcttct gaaattcttc ccaagaatga ttctggccgg cgaaacccct tcgccatagg
     1441 gagcaaaacg ctcttttttt tcaataaaaa tttccggcat cgcctggcga aaccggtaga
     1501 tgctctgttt tacatccccg accataaaca gattggtttc cccgcgtgag accgaacgaa
     1561 aaatggtatc ctgcgcgaga ttggtatcct gatattcgtc gatcataacc tcttcaaacc
     1621 gccgggacac ccgctcggcg atggaggtcg gaacaaatcc gccggccttc ttttcgacaa
     1681 gaagtctgag ggtaagctgc tcaagatcgc tgaaatccag aatattacgc ccgatcttct
     1741 cctcaaaaag ccgctgatcc aaatgaccga ctatttcaaa caggcaccga acaaccggat
     1801 aaagctgatt gatatctgac tggaattcct gctcatcaca gcatagaatt ccttttttta
     1861 aggaaacaag tgattttttt accgcgtccc tcaggccggt aaccctgctt ttaagccgct
     1921 cgtcttcgaa tttgcgcaga gggcccagtt tctgaaaacc gaattcgccg gccgcacggc
     1981 agacaccatc ccaatcgccg gattccgtaa ggcgaaggat tgaccttaga ccggaaagat
     2041 cgctttcgaa cgccggtccg taagccttga tcagccgttc atcccgatga atctccgaca
     2101 gggcctgctc ggtcagcgca atatcgttgc cgattgcaga gctgagataa tcaagagccg
     2161 gttttcccca ctcggtctcc gtaatttttc ggcccggccg gtacatctca agtttttcgc
     2221 tcagccaccc ctgcggatcc ggcagagaac ggacaaacct gtgcagcctg atgacagcgt
     2281 cggcaagctg ggtatcgtcc ctgcctgttc caagcatttc agaaaggatg atgaacggtt
     2341 tctcatcaat attatccacg ctgctgtccc gcaccgcgtc cgcgtaattt tcctccagaa
     2401 gctggtcgag cgtatcctgc agcagaacct tcatttcgct gtcgtccgca attctgaaat
     2461 ccggggaaag atcaagcttt tcaaaatttt cccggatcag gtcgaggcaa aaggcgtgca
     2521 ccgtgctgat atgggaattc tgaagtaaaa tctgctgccg ctgaagattg aggtcttcgg
     2581 gattttcggc gatcatctgc tccaaccgct gggatagcct ttcacgcatc tcggaggcgg
     2641 ccgcgttggt gaatgtcacc actaaaagcc ggtcagcgtc gcagggattc tcagaatcgg
     2701 tgatcttttg aatcacacgc tcgaccagca cggcagtctt tccgcttccc gccgcggcgg
     2761 agaccagaag cgtgcccgga gcggtgatgg cgtcgttctg atctttggtc cacactcttt
     2821 cagccatttt ccacgccccc ttcctcctta agcgatttcc agacttcttc tttgtcgtat
     2881 ttccggagca gacggatacc gtcccccggc tcgtggccac agacacacgc gtaatcgcag
     2941 taatcgcagg ggtggtagct gattccctga accggctctg ccgcgacgtc cccctcatgc
     3001 agagtcaggg ccatgtcttt gagcgttttg accacatggc gtttgagaaa accgaatttt
     3061 tcgagagaag ccaccgaggc tcgggaatcc ggttcataag aaacattgcc gtcggcatct
     3121 tcggattttc tgagctttgc gggaataaaa acgccggaac cgtcccgttc catcgcgcgg
     3181 atcacctccg gatcgtccag aagaagacca ctcattttat aggcgctgtc cctcgccgct
     3241 gaaatatctt cctcaccggc cgaccttgcc gctgtgatgt tcggccggcc ggaaggaaca
     3301 taaagtacgc cggcaggcgt gatctcaccc tgtgcggaat atctgccgcc cccgttgtca
     3361 cagagtgcga aaagataaag aaacatctgg agattaagac catagatcac atcggacaga
     3421 ttgaattcct tacttccggt tttataatca accactctga gataagaggc cccatcccgt
     3481 ttcatcaggt cgacccggtc gactttgccc tccaccgtta tgcttccccc gtcggggaga
     3541 tccaccgtca ggggtttcac atcccggccg atggtcagct caaaatccac aggccggaac
     3601 atgctttgct ccagctctct ggcaatatgt tcaatcaggc cgatcacctg accctccagc
     3661 cgcaggtaaa ggtatttaaa acgttccggc ttatccttaa gccccccgag aaaaccgtcg
     3721 gcaaactcct taagaaggct tttaacctgc gaggaaagtt cgtcccgggc aatagtccac
     3781 agcccttccc cgctatgttc cctcaacagc cgttcgagga caaaatggat cagcgtaccg
     3841 atttcaggcg catccatctc cgctcttccc ctcggaaacg ccctgagccc gtaacggcaa
     3901 taatagagaa accggcattg ccggaaggtt tccagccggc tgggcgaaat ctgcatccgt
     3961 tccccaaata aagccctggc gtttgtaaca tcgcgaaacg taaagggggt tttgtccgcc
     4021 gcccgcttaa gaaccgccat ccgccgctca tactccggct tttcggagaa aaaacgcgcg
     4081 attgcccggg tcatc
//
LOCUS       DATALY010000380         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_339834,
            whole genome shotgun sequence.
ACCESSION   DATALY010000380 DATALY010000000
VERSION     DATALY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339834"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VHR42_10395"
     CDS             complement(<1..612)
                     /locus_tag="VHR42_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX3027614.1"
                     /translation="MTGLGAVLTAVIAFLVTAVSGFSLIPYLKRLKFGQSIKEIGPVW
                     HKSKQGTPVMGGLMFILGILAAVLVLFAVFSGKIKDTDKIRLAGGVLMALVFGLIGFM
                     DDYIKVVKKRNLGLTAKQKLFLQLATGFVFVLVDMIYSGGRMLMTVPFFNVAINLRYP
                     IAYLLIALFIVVATVNSVNLTDGIDGLCSSVTFIAALAFMLTSK"
     gene            complement(614..1858)
                     /gene="murF"
                     /locus_tag="VHR42_10400"
     CDS             complement(614..1858)
                     /gene="murF"
                     /locus_tag="VHR42_10400"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX3027615.1"
                     /translation="MVSKALNLHAEEFQKGACAAVSQKTVDSPAGTVVMVDDTQKALL
                     QIAGAYRRLFDIPVVGITGSVGKTSTKEMVYAVLSEQYKTHKNEGNLNNQIGMPMSVF
                     GIGQDCEAAIFEMGMNSFGEISNLSKVAVPDIAIITNIGISHIEMLGSRENIRKAKLE
                     ILDGMKRDSTLIINGDDEMLSNISGRVGVKTMTYGVVNHACDAVADNILINENGTRFS
                     IIYRGECIPASVPAIGMHHVYNALAAFLAGVGLGMRPETAAAGMKNYKTAGMRQKIVK
                     RFGMTFIEDCYNASPASMSAAFEVLKVVGENRRIAVLADMLELGASARQAHLDVGKEA
                     AESGVDILFCYGDNAVFYCEGYHSAQKAGGKCMHFDSGKAVGDALSAILRPGDTVLFK
                     GSLRMNLEQTVAGIYQLLKGGN"
     gene            1849..>2035
                     /locus_tag="VHR42_10405"
     CDS             1849..>2035
                     /locus_tag="VHR42_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX3027616.1"
                     /translation="MKPIDLKLILEATGGYSDPECGNPVITGISTDTRKITQGCLYIA
                     LTGERFDGHDFIADAFRK"
BASE COUNT          531 a          555 c          463 g          486 t
ORIGIN      
        1 tttggacgta agcataaacg ccaaagcggc gataaaagtt accgaggagc acagaccgtc
       61 tattccatcg gtcagattca ccgagttaac tgtagcaacc acgatgaaca gggcgatcag
      121 caaataggcg atgggatacc gcagattgat cgcaacatta aaaaacggaa cggtcatcag
      181 catgcgtccg ccgctgtaaa tcatgtcaac cagcacaaac acaaaacccg tggcaagctg
      241 taaaaagagc ttttgcttgg ctgtcagccc cagattacgc tttttcacaa ctttaatata
      301 atcatccatg aatccgatca aaccgaaaac aagggccatc aggacaccgc ctgcaagcct
      361 gattttatcc gtatctttga ttttgcccga aaaaacggcg aaaagaacaa ggactgcggc
      421 aagaatgccg agaataaaca tcagccctcc cataacagga gtgccttgct ttgatttgtg
      481 ccagacaggg ccgatttctt tgatgctttg tccaaacttc agccgtttca ggtagggtat
      541 caaagaaaag cccgacacag ccgtgacgag aaaggcaatt actgccgtaa gtacggcccc
      601 caaacccgtc attttaattt ccacccttca gtagttggta tatcccggcg accgtttgtt
      661 caagattcat gcgcaggctg cctttaaaca gaaccgtgtc ccccggccgc agtatggcgg
      721 aaagggcgtc gcccacggct ttcccgctgt cgaaatgcat gcattttccc ccggccttct
      781 gggccgaatg atagccctcg caatagaaaa cggcgttatc tccataacaa aaaagaatgt
      841 cgacaccgga ttctgccgct tcttttccca catcaaggtg ggcctgtctc gcgcttgctc
      901 cgagctccag catatcggca agaaccgcga tcctgcgatt ttcgccgaca actttcaaaa
      961 cctcaaacgc ggctgacatc gacgcagggc tcgcattata gcagtcctca ataaaggtca
     1021 ttccaaaccg tttgacgatt ttctgtctca ttccggcagt tttgtaattc ttcataccgg
     1081 ctgcggcagt ctccggtctc attcccaaac caactccggc caaaaaggcg gccagtgcat
     1141 tgtaaacatg gtgcattccg atggccggaa ccgatgccgg gatacattct ccgcgataaa
     1201 taattgagaa acgggtgccg ttctcattaa taagtatatt gtccgccacg gcgtcacatg
     1261 catgattcac gactccgtaa gtcatcgttt tgaccccgac ccggcccgaa atatttgaaa
     1321 gcatctcatc atcgccgttg atgatcagcg tggaatcccg cttcatcccg tccagtattt
     1381 caagcttggc tttccgtata ttctcacggc ttcccaacat ttcaatatgg gaaataccta
     1441 tgttggtaat gatggcgata tcgggaacgg ctactttcga aagattggaa atttctccga
     1501 aactattcat acccatttcg aaaatggcag cttcgcaatc ctgcccgatc ccaaaaaccg
     1561 acatcggcat accgatttgg ttattgaggt taccctcatt tttatgggtc ttatactgct
     1621 ccgataaaac cgcgtacacc atttctttgg ttgaagtttt cccgacgctg cccgtgatgc
     1681 cgacaaccgg gatgtcaaac agccttcggt aggcgcccgc aatctgaaga agagcctttt
     1741 gcgtatcatc gaccatgacg accgtgcccg ccggagaatc caccgtcttt tggctgaccg
     1801 cggcgcacgc gcctttctgg aactcttccg catggaggtt aagagctttt gaaaccaatc
     1861 gatttaaagc tgattctgga ggcgacgggc gggtattccg acccggaatg cgggaacccg
     1921 gtgattaccg gaatcagtac cgatacaaga aaaataacgc agggctgtct ttatatcgcc
     1981 ctgaccggcg aacgatttga cggccatgat tttatcgcgg acgcgttccg gaaag
//
LOCUS       DATALY010000381         7802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_167972,
            whole genome shotgun sequence.
ACCESSION   DATALY010000381 DATALY010000000
VERSION     DATALY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167972"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1605
                     /locus_tag="VHR42_10410"
     CDS             190..1605
                     /locus_tag="VHR42_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEX3027617.1"
                     /translation="MTATTAENTGAVNASVKVILSTDSMTAVLWMTPPESGGNSVSGE
                     MIEKALKDAGVIQGIDKKLVEVLGKQPVYSKEIPIANGVDPVDGQNGKISYRFQISTQ
                     ARPKVNSDGTVDYHDLGIIQNVRKGDVLCDITPPTPGSDGFNVRGRVIKSKAGKPVSS
                     PNGKNTALSEDKSQLISTINGHVMIMGTKVVVNDTFIVSENVDNSTGNIKFVGNVSVI
                     GAIKEGFVVEAEGNVEVSGSILGGSIIAGGNITVRGGVVGMNRSTIKSKGDFSSTFLE
                     NCAVSAGGQVKASSIMNCNINCGRTMELTGYNSKLIGGHYVVGGDIIAHTIGSPSNIP
                     TDLVLGANPSLVTLQRSSSKQLKEIHSQIQKLEQIIRLLDRYKRINQLPPDKEEIYNN
                     SVYSYNDLQTKLQSLTEQVSAYNEEIMNSDKGKVVCKGTAYRGVKITIGFARLNILEP
                     VFSSSFKKEDTSITINNISPY"
     gene            2110..2256
                     /locus_tag="VHR42_10415"
     CDS             2110..2256
                     /locus_tag="VHR42_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027618.1"
                     /translation="MADAEAREDMEDMEDMAGMEDGQAGDRVFSAAGISERWARDSLL
                     FVLY"
     gene            complement(2462..2537)
                     /locus_tag="VHR42_10420"
     tRNA            complement(2462..2537)
                     /locus_tag="VHR42_10420"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2502..2504),aa:Ala,seq:ggc)
     gene            2694..3647
                     /locus_tag="VHR42_10425"
     CDS             2694..3647
                     /locus_tag="VHR42_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3027619.1"
                     /translation="MEIVILDGCAVNPGDLSWNDIEKFGNLTVYDNTPEELIVPRSED
                     AEAIFVNRVVLNREIIGSLKKLRFVGALGTGYNMIDLDACREKGVTVCNIPDYSTMSV
                     AQHVFALILHFTNSPASFHSAVLDGKWPGRREMNFLDYHFIELWGKTLGIIGLGSIGK
                     QVAKIASAFGMNVIATSRTQTEGIRDGINMASLEAVLGQSDILSLNCPLTDQTRGMIN
                     ENTIKQMKDGVILINTSRGPVIDEHALADALNKGKVRGAGLDVLTSEPPEKSCPLLTA
                     KNCLLTPHIAWATKEARTRLLKIVAENLEGYLNGKPVNRIV"
     gene            complement(3718..4398)
                     /locus_tag="VHR42_10430"
     CDS             complement(3718..4398)
                     /locus_tag="VHR42_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3027620.1"
                     /translation="MNFDMNFGQNDVNPTDLNGYGNVFGEIPGMDNVIADPMTAQTPE
                     QTAAVNPMGEDVMPGLMPDNRYSEQDDDMKNFMEQSYLMNPSCFGRYSAVYADQLYQF
                     INDEYRDHLYYLALSRKAPNNSSRHIFRNMAQDELKHARRFGAAYFLITGKKYFPTQS
                     MVGPVSVPSSYHQALRDRYIAESRDVVKYRQFARNTEDTCLKRMANNTSDDERSHAQE
                     IMELIQST"
     gene            complement(4490..6097)
                     /locus_tag="VHR42_10435"
     CDS             complement(4490..6097)
                     /locus_tag="VHR42_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX3027621.1"
                     /translation="MAKNTGKSLIKGTMILVIGNIIVKIIGALFKLPLANIIGADGMG
                     LYNASFIVFDIFLVLSTAGFPLAISKMVANSCAHRDGAEALKIFRVSRRIFAVMGIAF
                     TLIMALGAKTFSEMIGNTRAYYSILLLSPAILFVSLMSSYRGYYQGTNDMIPTTVSQV
                     TESLCRLIIGLSLSWYLKSAGYGTEIVAAGAIVGITAGEFASTFTLAMIHRRRMKRRR
                     VLKKCRTNSLAILKSMFSTAIPIGISTIIIAVINMLDNSVVMHRLQFIGNTEQQANTL
                     YGSFNMAFTVFSLPITIVSAITISVFPVLSYASACKNFPRVSKTSEASLRIAMIAATA
                     STALFASLSYPIIMLLYFNQPRDAKLAAPLLMLLAPSAITISMTMLSTVILQAIDKIM
                     VPSRSSIIGGAVCLASNWILVGNPKIGIYGAPVGIFICYAITSALNFRAIKKSGKVHL
                     SFRNLFFKPLIPAAVMALTGALSFNAFYASMGLLKSAAFSILLGLFNFIIVLFLNHTV
                     ERGDLVLLPKGTKIIRALEKVHLLDQI"
     gene            complement(6328..6810)
                     /locus_tag="VHR42_10440"
     CDS             complement(6328..6810)
                     /locus_tag="VHR42_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEX3027622.1"
                     /translation="MDSISKITVTEDVALITLRNSPADMKFVANAFDLIAQKSINVDM
                     ISQTAPLSERINLSFTISEEDLGAVIELLATLRADNPDIKSDISGSNCKISLYGEPMR
                     FTPGVAAQVFDTIASLNLDVRIITTSEIDISILIPQAEYNLAEDAFAKVFHHHILSIN
                     "
     gene            complement(6813..7577)
                     /gene="dapB"
                     /locus_tag="VHR42_10445"
     CDS             complement(6813..7577)
                     /gene="dapB"
                     /locus_tag="VHR42_10445"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX3027623.1"
                     /translation="MNRIILSGCNGKMGGVVSKTITENRDCVIVAGIDVKSGQQEDYP
                     VFSDPSGFQGKADVIIDFSNPGMLDCLLTFAKSRKIPIVIATTGFSRAQIQEIEAAAK
                     EIPVFFSANMSLGVNLMIEIAKKAAAVLSPNFDIEIIEMHHNQKVDAPSGTALMIADG
                     ISSVLDKEPQYVYDRHSQRKKREKTEIGIHAIRGGTVVGEHEVIFAGQDEMITIRHSA
                     MSKQVFAAGALKAAIFLMERKPGLYQMEHLVNTIGG"
     gene            7608..>7802
                     /locus_tag="VHR42_10450"
     CDS             7608..>7802
                     /locus_tag="VHR42_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027624.1"
                     /translation="MKNRTGTNPIRPVHRGKTKLLHCRKFPYNQIIANDFVLIQFLEF
                     PHFSQASEQEAYGTHRPHIQS"
BASE COUNT         2186 a         1733 c         1897 g         1986 t
ORIGIN      
        1 atggatgatc ccggatgttt tgcggaagcc gcgtgaaagg cgtattttta tatgccgttt
       61 cagtcacctt gtgatatcag ggtgactttt tttatttttt aacaaagttt tattaaaaaa
      121 ctaataatat taaaaaacca aacgatatac aataaggaga ttattattct tagaacggag
      181 ggtcttttct tgacagcgac aacagcggaa aacacaggag ccgtcaacgc ttctgttaaa
      241 gtaattctat ccactgatag tatgactgcc gtcttatgga tgactccacc ggaaagtgga
      301 ggaaattctg tttcgggcga aatgatagaa aaagcgctga aagacgcagg agtgattcag
      361 ggaattgata aaaagctggt cgaggttctc ggcaaacaac cggtttactc caaagagata
      421 cctattgcaa acggggtaga tcctgttgat gggcagaacg gaaagatcag ttaccgattt
      481 cagatatcaa cccaggcacg tccaaaggtg aattctgacg gtacggttga ttaccatgat
      541 ttggggatta ttcaaaacgt caggaagggg gatgttcttt gcgacatcac tcctccgaca
      601 ccgggcagcg atggattcaa cgtccgaggc agggtcataa aatcgaaagc gggcaagccg
      661 gtgtcttcgc cgaacgggaa aaacaccgct ctgagcgaag acaagtctca gttgatttct
      721 acaatcaacg gacacgtcat gattatggga accaaagtcg tagtcaatga tacgttcatt
      781 gtaagcgaaa acgttgacaa ttctaccgga aacatcaaat ttgtcgggaa tgtcagcgtt
      841 attggagcca ttaaagaagg ttttgtcgtt gaggctgaag gaaatgtgga agtcagcggt
      901 tcaattctcg gggggtcgat catcgctggc ggaaatatca ccgtaagggg cggcgtggtc
      961 ggcatgaacc gcagcacaat taaaagcaag ggcgatttca gcagtacttt tctcgaaaac
     1021 tgcgcagtca gcgcaggagg tcaggtcaaa gcctccagta tcatgaactg taatatcaac
     1081 tgcggacgga ctatggagct gacgggatac aattccaaac tcatcggcgg tcattatgtg
     1141 gtcgggggag atattattgc tcatacgatt ggctcgccct caaacatccc gactgatctg
     1201 gttctcggag caaatccatc tcttgtgacg ctccaaagat cttcgtcgaa acagcttaaa
     1261 gaaatccaca gccagataca aaaactcgag cagatcatcc gtctgcttga ccgttacaaa
     1321 agaatcaacc agcttcctcc cgacaaagag gaaatttata acaattccgt ttacagctat
     1381 aatgacctgc aaactaaact ccagagtttg acggagcagg taagtgccta taacgaagaa
     1441 atcatgaatt ccgacaaggg gaaagttgtc tgtaagggca cggcttatcg cggagttaaa
     1501 ataacaatcg gatttgcacg tttgaatatt cttgagccgg ttttttcttc ttccttcaaa
     1561 aaagaagaca catccatcac gattaataat atttccccct attaaaaaca aattacacaa
     1621 aaaaagttcc ctttaacgga aaaccgtaaa gggatcgaaa tgaaatagta ttagggggaa
     1681 aggtttggtt ccatgctttt atattacacg ataaatgtta agggcagtta aacgttgcgt
     1741 gaagtttatg tcaaattaac atgaagattg tgtgaagttc atagacagga agagcattgg
     1801 atctgggctg ctaatattta cttgacatac cgcaatatct atgatacact tagaaagtaa
     1861 atttaggatt tactttactc ttcttttcac gccaaaccga aggcatctta ttcggcggtg
     1921 atggtcacaa aactgtattg ccgacctgat caaaagcaga gactcacagt cgttaacccg
     1981 gacgtcacgc gggaccaaag aatacggcga tcgctaaatc ttagcgatcg ccttttagca
     2041 tctataaacc ccgcgggggg gtctttatat cattgatttt taatcaataa ggacgcggcc
     2101 tcggcggtct tggcggatgc ggaggcccgg gaggacatgg aggacatgga ggacatggcg
     2161 ggcatggagg acggccaggc cggtgaccgg gtcttctccg cggcgggaat atcggaaaga
     2221 tgggcaagag attccctcct ttttgtttta tactaaatta tattccaaaa aggcaggatt
     2281 gtttcggaca acatatgcgc agataattgc ttcaagtgtc ggcacaaagt ctttgtattt
     2341 tttcataaat acttcccgtt taaatttgat aagcgtgtgt atcgttcctt aaaaataaga
     2401 acataaagca aaacgcaccc cacaaaaaag tgaagtgcgc gatttgccac gatttaactg
     2461 ctggtggaga tgagggggat cgaacccctt acctcttgaa tgccattcaa gcgctctccc
     2521 aggtgagcta cacccccaca tggcgtatgt ttcctgccgc atttctgacg gcttgactat
     2581 aatatcatat tttttcgtcg aatgcaagat gttttttaaa aaacatggta ttgactttgg
     2641 ctttttttgc cgttaaagta tagaaagaga ataattaggt aaggtgtgat aatatggaaa
     2701 tcgtaattct tgacggctgt gcggttaatc cgggggactt aagctggaat gacattgaaa
     2761 aattcggaaa cctgaccgta tatgacaata ctcccgaaga attaatcgtt ccaagatcag
     2821 aagatgccga agcaattttt gtgaacaggg ttgtgctcaa tcgcgaaatt atcggttcac
     2881 ttaagaaact tcggttcgtc ggcgcgcttg gaaccggata taacatgatt gatctcgacg
     2941 cctgccgcga aaaaggcgtc accgtctgca acatccccga ttacagcacc atgtcggtcg
     3001 ctcagcatgt atttgcgctg attcttcatt ttaccaacag ccccgcatct ttccattcag
     3061 ccgttctgga tggaaaatgg ccgggaaggc gtgagatgaa tttccttgat tatcatttta
     3121 tagagctttg gggtaaaacg ctcggcatta tcggcctggg aagcatcgga aaacaggtcg
     3181 ccaaaattgc ttccgctttt ggtatgaacg taattgcaac aagccggaca cagactgagg
     3241 gaatccggga cggaattaat atggcctctc ttgaagcggt actcgggcag tcagatattc
     3301 tcagtctaaa ctgccctctg accgaccaga ccaggggaat gatcaatgaa aatacgatta
     3361 agcagatgaa ggacggcgta atactgatca acacgtcccg cgggccggta atcgacgaac
     3421 atgctctggc ggatgcgctc aacaagggta aagtcagggg agcggggttg gacgtattga
     3481 ccagcgagcc tcccgaaaag agctgtccgc tgctgacggc aaaaaattgc ctgttgacac
     3541 ctcatatcgc gtgggcgacg aaagaggcca gaaccagact gcttaaaatc gtagcagaaa
     3601 atctggaggg ttatctgaac gggaagccgg tcaaccggat tgtctgaccg ggcggcataa
     3661 acggtataaa acgggaaatc cggccgggcg aaagcctgcc ggattttttg ttgccaatta
     3721 agtactttga atcagttcca taatttcctg cgcatgactc ctctcgtcat cgcttgtatt
     3781 gtttgccatg cgtttcagac atgtatcctc ggtattcctc gcaaattgcc ggtatttgac
     3841 gacgtctctc gattcagcga tatagcggtc tctcaacgcc tgatgatagg aggatggaac
     3901 agataccggc ccgaccatcg actgtgtcgg gaaatacttt ttcccggtga tcagaaaata
     3961 agcggcgcca aatcttctcg catgcttgag ctcgtcttgc gccatattgc ggaaaatatg
     4021 acgagaggaa ttattgggcg cctttcttga caacgcgaga taataaagat gatcccgata
     4081 ttcatcattg atgaattgat aaagctggtc tgcgtaaacg gccgaatatc tgccaaaaca
     4141 gctgggattc atcaggtagc tttgttccat gaagtttttc atgtcgtcgt cctgttcaga
     4201 ataccgatta tccggcatca gtccgggcat tacatcttcg cccataggat tcacggcagc
     4261 cgtctgctca ggggtctgag cggtcatagg atcggcgata acgttatcca tcccgggaat
     4321 ttcgccaaag acatttccat aaccgttcaa atccgtggga ttgacgtcgt tttgaccgaa
     4381 attcatatcg aaattcataa atatcctccc gcctgttatt tattatgcaa tatcagaata
     4441 tgagcgtcgg agcgatattg ttcatcagaa aaaagccggg aggagaatgt tatatttggt
     4501 caagcaagtg gactttttca agcgcccgaa taattttggt tcctttcggg agaaggacaa
     4561 gatcgcccct ttccaccgtg tgattaagaa acagcacgat tataaaatta aacagtccaa
     4621 gaagaatact gaatgccgcc gacttgagaa gccccattga agcataaaag gcattaaaag
     4681 acaaggctcc ggtcagcgcc atgacagccg cgggaatcag aggcttgaaa aacagattgc
     4741 ggaaagaaag atgaactttt ccactttttt taattgctcg gaaattgagt gccgaggtaa
     4801 ttgcgtaaca gataaagatt cccaccggcg ctccgtagat tccgattttg gggttgccga
     4861 ccagaatcca gtttgacgca aggcatacag ccccgccgat aattgaggaa cgggacggaa
     4921 ccatgatttt gtcaatcgcc tgaagaatta ccgtggacag cattgtcatg gatatcgtga
     4981 ttgccgacgg ggcgaggagc atcagcaggg gagccgcaag ctttgcgtcc ctgggctggt
     5041 tgaaatacag gagcatgatg atcggatacg acagggacgc gaagagggct gtggacgccg
     5101 ttgcggcgat cattgcaatc cgcagcgagg cctccgacgt tttgctgacc ctcggaaagt
     5161 ttttgcaggc ggaggcgtag gaaagaacag ggaaaacgct gattgtaata gcggaaacga
     5221 ttgtgatggg caggctgaaa acagtaaaag ccatattaaa tgaaccgtaa agggtattgg
     5281 cctgctgttc ggtgttgccg ataaactgga gccggtgcat aacgaccgaa ttatccagca
     5341 tattgatgac agcgatgatg atggtgctga tcccaatggg gatggcggta gaaaacattg
     5401 atttgagaat cgccagagaa tttgtccggc attttttcag aacgcgcctg cgcttcatcc
     5461 gccggcgatg aatcatcgca agagtgaacg tggaggcgaa ctcgcccgcc gttatgccga
     5521 cgatggcccc ggcggctaca atctctgtgc cgtatcccgc acttttcaga taccatgaga
     5581 gtgaaagacc tataatcagc cggcagaggg actccgtcac ctgcgaaaca gtagtgggta
     5641 tcatatcatt ggtgccttga taatatcccc gataggaaga catcagcgat acaaacagaa
     5701 tggcggggga gagaaggaga atcgaataat aagccctggt atttccgatc atttcggaaa
     5761 atgtttttgc gccaagagcc attataagtg taaaggctat acccattaca gcaaatattc
     5821 gtctggaaac ccggaatatt tttaatgctt ccgcgccatc ccgatgggcg cagctgttag
     5881 cgaccatttt ggagatggca agcgggaagc ctgcggtgga cagcacgagg aaaatatcaa
     5941 agacaataaa agaagcgtta tacagcccca taccgtccgc accgataata tttgccagcg
     6001 gaagtttaaa cagagccccg atgatcttga caatgatatt gccgatcacc agaatcattg
     6061 tgccttttat cagacttttg ccggtgtttt ttgccattcc gtactcctcc agataattgg
     6121 agcccctcat atttggaaac aggcctttgg cgtccatata taagatttat tcgcgaacag
     6181 gccggggaat gacagtttac ttagccggcg ggataaaatc gaatagagcg ggccttaatt
     6241 ttaacgaaaa aaaagccata tcggtcgatt gaccaataca gcttttttca aacagcgcct
     6301 ccgccgttct ataacggagg gcgttcttta attaatggat agaatatgat ggtggaagac
     6361 ctttgcaaaa gcgtcttccg cgagattata ttccgcctga ggaatcagga tggaaatatc
     6421 aatctcagat gtagtgataa ttctcacatc gagattcaga ctcgctatgg tatcgaatac
     6481 ctgcgccgct acgccgggcg taaacctcat aggctcacca taaagcgaga tcttgcagtt
     6541 actgccgctg atatccgatt taatatcggg gttatcggcc cggagagtgg ccagcagttc
     6601 gataaccgcg ccgaggtctt cctcggatat ggtaaacgac aggttgattc tctcgctcag
     6661 cggggcagtt tgtgaaatca tatcaacgtt tatgcttttt tgagcgatca aatcaaaagc
     6721 gtttgcgaca aacttcatat ctgccggcga atttctgagt gtgatcagag cgacgtcttc
     6781 cgtcactgtg attttgctga ttgagtccat cgttaacctc ctattgtatt gacaaggtgc
     6841 tccatttgat agagccccgg ttttctttcc atcaaaaaga ttgcggcttt cagtgcgccg
     6901 gccgcgaata cctgcttgga cattgccgag tgccggatgg taatcatctc gtcctgaccg
     6961 gcaaaaatca cttcatgctc gccaaccacc gtgccgcctc gaatcgcgtg aattccaatt
     7021 tcggttttct cgcgtttttt ccgctgcgaa tgtcgatcat agacatattg aggctctttg
     7081 tccaaaacgg atgaaatacc gtccgcaatc atgagagccg ttccgcttgg agcatcgacc
     7141 ttctggttgt ggtgcatctc tatgatttca atatcaaaat tcgggcttaa caccgccgcc
     7201 gctttttttg caatttctat catcaggttg acgccaagtg acatattggc agagaaaaag
     7261 acgggtattt cttttgcagc cgcctcaatt tcctggatct gcgcgcggct gaatccggtc
     7321 gtggcaatca cgataggaat ttttcggctt tttgcaaaag tcagaagaca atccagcata
     7381 ccgggattgg aaaaatcaat gataacatcc gcttttccct gaaacccgga aggatcggag
     7441 aaaacagggt agtcctcctg ttggccggac tttacgtcga ttcccgcgac aataacgcag
     7501 tcccggtttt ccgtaatggt ttttgaaacc acgccgccca tttttccgtt gcatccgctt
     7561 aaaataatgc gattcatttt aacctccata cgcccggagc gtattctttg aaaaaccgca
     7621 cggggacgaa tccgatccgc cccgtgcaca gggggaaaac aaaattatta cattgccgaa
     7681 aattccccta taatcagatt atagcaaatg atttcgtttt aattcagttt ttagaatttc
     7741 cgcatttttc tcaagcatcg gagcaagagg catacggcac tcaccgaccg catatccaat
     7801 ca
//
LOCUS       DATALY010000382         6307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_340479,
            whole genome shotgun sequence.
ACCESSION   DATALY010000382 DATALY010000000
VERSION     DATALY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340479"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VHR42_10455"
     CDS             <1..261
                     /locus_tag="VHR42_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX3027625.1"
                     /translation="RWEEEESYAVEDQLPMGHGEKSVYLAMKFIRLTDGEALAIRWHM
                     GGYDDAVRSFGSGRAQSNAYRAYPLAAALNIADMYVSYIINR"
     gene            443..592
                     /locus_tag="VHR42_10460"
     CDS             443..592
                     /locus_tag="VHR42_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEX3027626.1"
                     /translation="MATWIIAGLIAGFTVYIIGKSVRKIWKGDSGCSGCCSQCTGCRP
                     VEKTK"
     gene            760..1623
                     /locus_tag="VHR42_10465"
     CDS             760..1623
                     /locus_tag="VHR42_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX3027627.1"
                     /translation="MYQILNKANLNPTVTKMVITAPFVARKTQPGQFVILRIDEKGER
                     LPFTVADYDREKGTVTIIFQQVGKATKQLGLLGEGDEIMDFVGPLGMPTPLEGIRRAA
                     VVGGGLGCAIAWPQARKLFDSGAKVDIIAGFRNRDMVILEDEMRACSDRFFLCTDDGS
                     YGEKGLVTNILERLIAEGAHYDCVIAIGPLPMMKFVSRLTKQYQIRTIVSMNPIMIDG
                     TGMCGCCRVTVGGKTKFACVDGPDFDGHQIDFDEAMARLSTYKKEETRELDHYCRMME
                     QADGLEQQPGR"
     gene            1668..3062
                     /gene="gltA"
                     /locus_tag="VHR42_10470"
     CDS             1668..3062
                     /gene="gltA"
                     /locus_tag="VHR42_10470"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX3027628.1"
                     /translation="MPNMSNRKVPVPEQEPLKRNKNFEEVSLGYTREMAEEEAQRCLH
                     CKHRPCVSGCPVNVRIPEFIALIAQGDAAGAFEKIHETNSLPAVCGRVCPQESQCEAK
                     CVRGIQGEPVAIGRLERYAADFNRQNSPQRPKKAETNGHKVAVVGAGPSGLTCAGDLA
                     KLGYEVTVFEAFHVPGGVLMYGIPEFRLPKAIVENEIDNLRALGVNIMTNMVIGKTLT
                     VDELFHDMGFEAVFIGSGAGLPRFMGIPGEGLVGVYSANEFLTRVNLMKAYRDDYDTP
                     VKKAGSVAVIGGGNVAMDAARCAKRLGAETVSIVYRRSEEEMPARKEEIHHAKEEGIR
                     FHLLTSPVAIHGNEKGAAEELECIEMELSEPDASGRRAPVPIKGTNFTLKADAVIMAL
                     GTSPNPLLGRTTPGLETDRRGCILVDEDSMMTRRDRIYAGGDAVTGAATVILAMGAGK
                     QAAIAIDGQIRGKR"
     gene            3614..5434
                     /gene="glmS"
                     /locus_tag="VHR42_10475"
     CDS             3614..5434
                     /gene="glmS"
                     /locus_tag="VHR42_10475"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX3027629.1"
                     /translation="MCGIVGYVGGRDSAEVLLDGLSSLEYRGYDSAGIAVFEKDSIKV
                     VKSKGRLKNLRDKLEAEGTPSGSCGIGHTRWATHGAPSDVNAHPHSGERVTIVHNGII
                     ENYSEIKKKLIEKGCRFESDTDTEVAAKLIDYYYQSDPFEAIRKALLRIRGSYAFGIL
                     FREYPNRIYAVRKDSPLIVGAGKGENFIASDVPAILKYTREYYLLEENEVAVLTGDEV
                     NIFDIGGEPVKKEKLTANWDVAAAEKGGFEHFMLKEIYDQPKAIRDTVSPRLIGGLPA
                     FNEGMHGADCFEHVGKVRIVACGTAMHAGMVGKYAVESLARIPVEVDIASEFRYRNPI
                     FASDELVVIISQSGETADTLAALRLAKGQGIRTLAIVNVVGSSIAREADGVIYTWAGP
                     EIAVASTKAYSVQLSVMYLFAIRLALAAGAMNESEAASLCKEIEKLPDVVQKLLCCKE
                     QCQHLASLYQNSRSLFFIGRGMDYASSMEGSLKLKEISYLHSEAYAAGELKHGTISLI
                     VDGTPVVAVATQKNLFEKTVSNIKEVKARGAHVLLVCREGEKEVDKIVDHVIELPNMP
                     DILMPSLSVIPLQLFAYYMAVERGCDVDKPRNLAKSVTVE"
     gene            5715..6053
                     /locus_tag="VHR42_10480"
     CDS             5715..6053
                     /locus_tag="VHR42_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3027630.1"
                     /translation="MVRSYADFALLCKALSDETRLQIIDMLSCGELCACDILKPFHIT
                     QPTLSYHMKILSECGIVNADRRGAWMHYTINDDMIKRISEYWKHLTSDKENCICRTCS
                     EKSDECDCEE"
     gene            6068..>6307
                     /locus_tag="VHR42_10485"
     CDS             6068..>6307
                     /locus_tag="VHR42_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA synthase subunit gamma"
                     /protein_id="HEX3027631.1"
                     /translation="MESNVNQTTDKCSCSSGCCGSTKPLPIPKAPYIRGLNLTMVGAV
                     PRITTELASRDILGAWKVRWGIGRMNYKVAPGLYSV"
BASE COUNT         1589 a         1394 c         1838 g         1486 t
ORIGIN      
        1 cggtgggaag aggaagagtc ctatgcggtg gaagatcagc ttcccatggg gcacggggaa
       61 aaatccgttt acctcgcgat gaaatttatc cggctgaccg acggggaggc gctcgccatc
      121 cggtggcata tgggcggcta cgacgacgcc gttcgttcat ttggaagcgg gcgtgcccag
      181 agcaatgcgt atcgcgctta tccgcttgcg gccgcgctga acatcgccga tatgtatgtt
      241 tcttacatta tcaaccggta gaccggctga tccccaaaaa atacctatgt ttctgtgagg
      301 cctgaacact gtttcaggcc tttttttggg ttacgcccta tttgaggctg ccgttagaat
      361 ccggttgaca gggctggcat cttttgctat actataaatg aaaacgataa tcattatcaa
      421 tagcagaaaa gagtgatgca aaatggcaac ctggattata gcgggactga ttgcgggatt
      481 caccgtctat attatcggaa aaagtgtaag gaagatctgg aaaggggatt ccggctgttc
      541 gggttgctgt tcccaatgca cgggctgccg cccggttgaa aaaaccaaat aaaatccgat
      601 cagatattcc cccgaatcgt tttgaatatg atataataaa tcagacggct gtaaacctgt
      661 cggggtatgg ccgtctgacc tttattgccg cctgtggctt ggaaaccgcc cgcggctgtt
      721 aaaatttaaa tgataaaaaa ctgaaatgcc ggtgatgtca tgtatcagat attgaataaa
      781 gccaatttaa atccgacggt tacaaaaatg gtgattaccg ctccgtttgt cgcgcgaaaa
      841 acacagccgg ggcaattcgt tatccttcgg atcgacgaaa aaggagagcg cctgccgttt
      901 accgttgcag attatgaccg cgaaaaggga acggtaacga tcatatttca gcaggtgggg
      961 aaggcaacca aacagttggg actgcttggt gagggagacg aaatcatgga tttcgtcggc
     1021 ccgctgggga tgccgacgcc tctggaggga atccgcaggg ctgcggtggt cggaggcggg
     1081 ctcggctgcg ccattgcctg gccgcaggca agaaagctgt ttgattcggg cgcaaaggtc
     1141 gatatcatcg ccggtttccg caatcgggat atggtgattc tggaagacga aatgagagct
     1201 tgttccgatc gcttttttct ctgcaccgac gatggttcct atggtgaaaa ggggctggtg
     1261 acgaacattt tggaaaggct gatcgcggaa ggcgctcatt atgattgcgt cattgccatc
     1321 ggcccgctgc cgatgatgaa gtttgtctcg cgcctgacca aacaatatca gatcagaacg
     1381 atcgtcagca tgaatccgat catgattgac gggactggga tgtgcggctg ctgccgcgtt
     1441 accgtcgggg gcaagacaaa gtttgcctgt gtcgacgggc cggattttga cggtcatcaa
     1501 attgattttg atgaagctat ggcccgcctg tctacttata aaaaagaaga aaccagggag
     1561 cttgaccatt attgcagaat gatggagcag gcggatggat tggaacagca gcccggcaga
     1621 tagaacgggt tttcaaaaga ttaccgcgat gtcacaggga ggtaaatatg ccgaatatga
     1681 gcaatcgaaa ggttcccgtg ccggaacagg agcctttaaa gagaaataaa aattttgaag
     1741 aagtgtcgct gggttatacc cgcgaaatgg cggaggaaga agcgcaaaga tgccttcact
     1801 gcaagcatcg gccgtgtgtt tccggctgcc cggtgaatgt cagaataccg gagtttatcg
     1861 cgctgatagc ccagggagac gcggcgggtg cgtttgaaaa gattcatgaa accaattcac
     1921 tgccggcggt atgcggacgg gtttgcccgc aggaatccca gtgtgaggcg aaatgcgtca
     1981 gggggattca aggcgagccg gtagccatcg gcaggctgga acgttacgcc gccgatttca
     2041 atcggcagaa ttcccctcag cgccccaaaa aagcggaaac caacggtcat aaggttgcgg
     2101 ttgtcggcgc cggcccttcg gggctgacct gcgcgggcga tcttgcgaag ctcggctatg
     2161 aggttacggt ttttgaagcc tttcatgtcc cgggcggcgt gctgatgtat ggaattcccg
     2221 aatttcggct tccgaaagca atcgttgaga atgagatcga caatctccgc gcgctcgggg
     2281 taaacattat gaccaatatg gtgatcggca aaacactgac ggtggatgaa ctgtttcacg
     2341 acatgggctt tgaggctgtt ttcatcggct caggcgccgg tctgccgaga tttatgggaa
     2401 taccgggcga agggcttgtc ggggtatatt cggcaaatga attcctgacc cgcgttaatc
     2461 tgatgaaagc atatcgcgac gattacgata cgccggtgaa gaaggccggg tctgtggccg
     2521 taatcggcgg cggcaacgtg gcgatggacg ctgcccgctg tgcgaagcgg ctgggcgcgg
     2581 agacagtatc catcgtttac cgccggagcg aagaggaaat gccggccaga aaagaagaaa
     2641 tccatcatgc aaaggaagaa ggcattcggt ttcatctgct gacttctccg gttgcaattc
     2701 atggaaatga gaaaggcgcg gctgaagaac tcgaatgcat cgaaatggaa ctctccgagc
     2761 cggatgcttc cgggcgccgg gctccggtac cgataaaagg aacaaatttt acgctgaaag
     2821 cggacgctgt catcatggcg ctcggaacct ctccgaaccc gctgctcggg cgaacgacgc
     2881 ccggacttga aaccgacagg cgtggctgta ttcttgtgga tgaagattcg atgatgaccc
     2941 gcagagatcg gatttatgcg ggaggcgacg ccgtaaccgg agcggcaacg gtcattcttg
     3001 cgatgggcgc gggcaagcag gcggcgatcg cgatcgacgg gcaaatcagg ggaaagcgct
     3061 gatcaggaag attggttgga tatttcagag ggggacttcc tgcgggcagg cattatgcct
     3121 accgcggccg tccctctctt ttgatccgat ttgtgtttgt cgggatgatt tctcaccaaa
     3181 acaggttgaa ttcgatatga tggaatgtaa ccggttatca gcttttgcaa aggacaatca
     3241 tacggttttt cttgacattt cgtcagggta gtgtatcata ataatgtctg attaaagtcg
     3301 gcggctattg ttctcggtta aaatcggccg ttagcgggga agcggggctt taaaatcaga
     3361 tgtttatagc aagggaggga agccgatgtt atctgacggc ccgtgaagac gaggctgaaa
     3421 attgaatatt ctcagcgcca ggaccggcta tcgcgccggt tgacgaggtg gggggttaat
     3481 cgaaaaattc ggcgggtgcc cctccggctg ttagtgttgc agccgtgagt cttcctgtaa
     3541 aaaacgcggg taatcgcgca acaaagggaa gatagcagcg ctataattat gccattttgg
     3601 aggtttacat aatatgtgtg gaatcgttgg ttatgttggc ggcagggatt cggcagaggt
     3661 gttgctggat ggtctgagca gcctggaata cagaggttat gactcggcgg gaattgcggt
     3721 ttttgaaaag gattcgatca aagtggtcaa atccaaaggc cgtctgaaaa acctgagaga
     3781 taaacttgaa gcggaaggaa caccttcggg aagctgcggt atcggccata ccagatgggc
     3841 gacccacggc gcgccttccg atgtgaatgc ccatccccat tcgggtgagc gcgttaccat
     3901 tgttcataac ggaatcattg aaaactatag tgaaatcaag aaaaaactga tcgaaaaagg
     3961 ctgccgtttc gagtcggata ccgatacgga agttgcggcg aagcttattg attactacta
     4021 tcagtccgac ccgtttgaag cgattcggaa agcgctgttg agaatacggg gttcctatgc
     4081 gttcggaatc ctgttccggg aataccccaa ccggatttac gctgttcgaa aggacagccc
     4141 gctgattgtc ggagccggaa aaggcgaaaa tttcatcgcg tccgacgttc ctgcaatttt
     4201 aaagtacaca agggaatact atttgctgga agaaaatgaa gttgcggtgc tgaccgggga
     4261 tgaagtgaat atctttgata ttggcggaga gccggtaaag aaagagaagc tgacagcgaa
     4321 ctgggatgtg gccgcagcgg aaaaaggcgg ttttgagcat tttatgctca aagagattta
     4381 tgatcagccc aaggcgattc gagatactgt gtcgccccgc ctgatcggag gtcttccggc
     4441 gtttaatgaa ggaatgcacg gtgcggattg ttttgaacat gtcggcaaag tccggatcgt
     4501 tgcatgcgga acggcgatgc atgcgggcat ggtcggcaaa tacgccgtcg agtcgctggc
     4561 aagaatcccg gttgaggttg atattgcgtc tgaattccgt taccgaaacc cgattttcgc
     4621 ctccgacgag ctggtggtga ttatttccca gtctggtgag acggcggata cgctggcggc
     4681 gctccgtctg gccaaaggtc agggaatccg aaccctcgcc attgtgaatg tggtcggctc
     4741 ctcgattgcg cgcgaagccg acggcgtcat ttatacctgg gcagggccgg aaattgccgt
     4801 ggcatcgacc aaagcctatt cggttcagct gtcggtgatg tatctgttcg caatcagact
     4861 ggccctcgcg gcgggtgcaa tgaacgagtc ggaggccgct tcgctctgta aggaaataga
     4921 gaaactgccc gatgtggttc aaaagcttct gtgctgtaag gaacaatgtc agcatcttgc
     4981 gtcgctctat caaaattccc gcagcttgtt ctttatcggc cgcggtatgg attacgcgtc
     5041 gtcgatggaa ggttccctga agctcaagga gatttcctat cttcacagcg aggcctatgc
     5101 ggctggggag ctcaaacatg gaaccatatc gctgatcgtc gacggaacgc cggtcgtggc
     5161 ggtggctacg cagaaaaacc tgtttgaaaa aacggtcagc aatatcaagg aagtgaaagc
     5221 gcgcggcgcg catgtcctgc tggtttgccg cgaaggggaa aaagaagtcg acaagatcgt
     5281 cgatcatgta atcgagctgc cgaatatgcc ggatattctg atgccgtcgc tgtcggtgat
     5341 tcctctgcag ctgttcgctt attatatggc ggtcgagcgc ggctgtgacg tggataaacc
     5401 gcggaacctt gccaagtccg tcacggtaga ataaacggtg acgcttgaaa agccgctttc
     5461 gcgcagaaac taaaattgaa catgcgtaac atcgtatgat caattgcaac gatgtttgaa
     5521 caaaattgtg cggtcaactt gttaagttat aatcagatcc tgtttgtgct cttgatttgt
     5581 gatgatttag agccaaaaca gggtctgatt tttaggatca aatttataaa tttgcattga
     5641 caacatgggt atttcgggca tattatatag ataacaataa atgttatata agcttgtcta
     5701 taaaggagga cattatggtt agaagctatg cagattttgc actgctttgt aaggctctgt
     5761 ccgatgaaac acgcctgcag attattgata tgctttcatg tggggagctt tgtgcatgcg
     5821 atattttaaa accgtttcat ataacgcagc caacgctttc ctaccacatg aaaatccttt
     5881 ctgaatgcgg tatcgtcaac gccgaccggc gaggtgcatg gatgcattac acaataaacg
     5941 acgatatgat aaagcggatt tcagagtatt ggaaacatct gaccagtgac aaggaaaatt
     6001 gtatctgccg cacatgcagt gaaaagtccg acgaatgcga ttgtgaagaa taatgggagg
     6061 cgataggatg gaatcgaatg tgaatcaaac gaccgacaag tgttcctgct cttccggctg
     6121 ctgcggcagc accaaacctt tgcctattcc gaaagcacct tatatcaggg gcctgaatct
     6181 gacaatggtc ggtgcggttc cgcgtatcac aacggaactt gcttcgaggg acattctggg
     6241 cgcatggaaa gtgcgctggg gcattgggcg catgaattat aaggtcgctc ccggcctgta
     6301 cagcgtg
//
LOCUS       DATALY010000383         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_426968,
            whole genome shotgun sequence.
ACCESSION   DATALY010000383 DATALY010000000
VERSION     DATALY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426968"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VHR42_10490"
     CDS             complement(<1..254)
                     /locus_tag="VHR42_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3027632.1"
                     /translation="MKQQSTSKGFAILSIASILCKVLSLVYVPLQTAIVGYSGNGMIS
                     SGYRIYIFMFSLSNAGLPIALSKLISESDAVGNYKGSKKI"
     gene            454..1110
                     /locus_tag="VHR42_10495"
     CDS             454..1110
                     /locus_tag="VHR42_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX3027633.1"
                     /translation="MSGIQLVIEVFLHLDKHLGTFMGQYGVLAYVALFLIIFCETGLV
                     VTPFLPGDSLIFAAGALAAAGIMTPYVVLLLIAAAILGNTVNYEIGRFLSEKIQRREN
                     IRFIKMEYIDRTHRFFEKYGGITIIITRFVPIIRTFSPFVAGVGKMPYKRFLSFNAIG
                     GISWATLFFVVGYFFGNLPFVKSHFSFIVIGIILVSLLPAVIAFFKARQPGKKQEEEE
                     "
     gene            complement(1119..2288)
                     /locus_tag="VHR42_10500"
     CDS             complement(1119..2288)
                     /locus_tag="VHR42_10500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX3027634.1"
                     /translation="MAEVTLRRGEENDIYKGHLWIYDNEVEGIRGEFEPGDIVDVVSA
                     RGRFLGRGYINPKSKILVRLMTGKKEEIDRGFLKRKIEDAVNLRKKLGLDSAYRVVFG
                     EADYLPALIVDKFADCLVIQTLALGIDRMKKDIVEILSECYHPRCIYERNDVPIRRKE
                     GLELTTGALFGQSPGVVEIEENGIRMLVDIENGQKTGYFLDQRENRAAIEPYVRGCEV
                     LDCFCHTGGFALHAAKFGASAVEAVDISEPALEFVKQNAALNGFETITAKQANVFDLL
                     KGYQDQKRLFDTVVLDPPAFCKTKSALPGAYRGYKEINLRAMKLIRPGGFLITCSCSH
                     FMTPDLFLKMLSEASADVRVASRIIEIRYQAKDHPVAVNADESLYLKCVILQILG"
     gene            complement(2443..3732)
                     /locus_tag="VHR42_10505"
     CDS             complement(2443..3732)
                     /locus_tag="VHR42_10505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3027635.1"
                     /translation="MKILRKIKTALSVFRRQGFREVCRLGVKKAAFLLRYVLHCAAGG
                     FYRLGFRKVFRMFRKNSKPRVLYVTSRFEAEGGGQTARYRIHNLIEALRGKAEAAFEV
                     ADSGIALDDCAIDRADVIVLMRLDWSEHTDGLIRRAKKWRKPLVFDIDDIIFLPEYIN
                     RFCQAIEDHSEETVRLYRETFLNFEKTFRQCDYATASTPFITQKMEQAGKRAFVIHNG
                     LNDKQLGIASGLEFGGMAKRNVNYLAYLSGSKTHDRDFRQALPAVVRILREYPDLRLR
                     IAGYLDLEGWPEDLIGRVDHAGYMKYTQLLKYSAENFINLAPLDTENPFCHAKSELKY
                     FEAAIVGVPTVASPTDTFVRCIVSRENGLLASTEEEWYQSIKSLIDDEVLYQTIAENA
                     RKHALDNYSPPAIGRQAGEAYSRILQLYREECSESGR"
     gene            complement(3729..>4223)
                     /locus_tag="VHR42_10510"
     CDS             complement(3729..>4223)
                     /locus_tag="VHR42_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027636.1"
                     /translation="AGVPALGFDIGAIGGRIRKTGFGYLLPFGADERQILQKIGEVMD
                     DRAGLLEIRDRLLGYQPKSIAEMGAEYAALYQKVILKEGKPPAAEVDNRMISQAIARA
                     AEGTETMGIAYGARLEAMRERIAKMLPGFISKRILKGQYPFKQQLKRLAARLSGKNGT
                     GKQK"
BASE COUNT         1020 a         1139 c          957 g         1107 t
ORIGIN      
        1 aatatttttt tggagccttt ataattgccg acggcgtcgc tttcggaaat cagtttcgag
       61 agcgcaatcg gcagccccgc gttagacagg gaaaacataa aaatataaat ccggtagccc
      121 gagctgatca tcccgttgcc ggaatacccg acaatggcag tctgcagtgg cacataaacc
      181 agcgacagca ctttacatag gatgctcgcg atgcttaata tggcaaaacc tttcgaggta
      241 gattgctgtt tcatgatatt tctccgttaa atagattccg catgcgacgg gattggcaaa
      301 caggacggca tcattgttaa ggtcaatcca ttaaaaccca atgagattaa tatacaccgg
      361 gcggcacacg aaatcaatcc atattgaatt attggtgaaa tttctccgat tttatgatat
      421 gatagaagaa atctgtttca gaaggagact gttatgtcag gtattcagct ggttattgag
      481 gtttttctgc atctggacaa gcacctcggc accttcatgg gccaatatgg ggtgctggcc
      541 tacgttgccc tttttttaat cattttctgc gaaacgggcc ttgtagtcac accgtttctg
      601 ccgggtgatt cgctgatttt cgcagccggg gcccttgcgg cggcagggat catgacccca
      661 tatgtcgtat tgctgctgat tgcggccgcc attctcggca ataccgtaaa ctatgaaatc
      721 gggagattcc tgagcgaaaa gattcagcgc agggaaaata tccggtttat taagatggag
      781 tatattgacc ggacgcaccg gttctttgaa aaatacggcg ggatcacgat catcatcaca
      841 agatttgttc cgattatccg tactttctct ccttttgtcg cgggcgtcgg aaaaatgccc
      901 tacaaaagat ttttaagttt taacgctatc ggcggaattt cctgggccac actatttttt
      961 gtcgtaggct atttcttcgg aaatcttcct tttgtgaaaa gccatttcag tttcattgtg
     1021 atcggaatca ttctggtttc cctgcttccc gctgtgatcg cgttctttaa ggcaagacag
     1081 ccgggcaaaa aacaggagga agaagaataa tccccctgtt aaccgagaat ttgcagaatc
     1141 acgcatttca aataaagtga ttcgtccgca ttgaccgcta ccgggtgatc tttcgcctgg
     1201 tagcggattt ctattatcct gctggcaacc cggacatccg ccgaggcttc gctgagcatt
     1261 ttcagaaata aatccggcgt cataaaatgc gagcaggagc aggtaatcag gaatcctccg
     1321 ggtctgatca gtttcatcgc ccgcagattg atttccttat agccgcggta tgcgcccggg
     1381 agtgccgatt tggttttgca gaaagccggt ggatccagaa caaccgtgtc aaaaaggcgt
     1441 ttttgatcct gatagccttt gagcagatca aaaacattcg cctgttttgc cgtaattgtc
     1501 tcaaaaccgt tgagcgcggc attttgtttg acaaattcca acgcgggttc cgaaatatcc
     1561 acagcctcga cggctgaggc accgaatttt gcagcatgaa gggcaaaacc tccggtatga
     1621 cagaaacaat ccagcacctc gcagccccgg acataaggct cgattgcggc cctgttttcc
     1681 cgctggtcga gaaaataacc ggttttttgt ccgttttcaa tatcaaccag catcctgatg
     1741 ccattttcct cgatttccac gacgccgggg ctttgaccaa aaagcgcgcc ggtggtcagc
     1801 tcaagccctt cctttcgacg gatcggaacg tcgtttctct cataaataca gcggggatga
     1861 tagcattccg agaggatctc cacaatatct tttttcatcc ggtcaatacc caaagcgagc
     1921 gtctggatga ccagacagtc ggcaaacttg tcgacaatca gcgcgggcag ataatcggct
     1981 tcgccaaaaa cgacccggta ggcactgtca aggccgagtt ttttcctcag gtttaccgcg
     2041 tcttcaatct tccttttcag gaatccccgg tcaatctctt ctttttttcc tgtcataagc
     2101 ctgaccagta tctttgattt cgggttaata tagcctcttc ccaagaaccg gccgcgggcc
     2161 gaaacaacat ccacaatatc gccgggctca aattctcccc ggattccctc cacctcattg
     2221 tcgtaaatcc agagatggcc tttataaata tcgttttctt cgccccggcg caatgtaact
     2281 tccgccatat ttaaaatcct ccgaatcgta ttctgtccta atatttcttt atccgtgttt
     2341 agccgccgcg attcggcgca aggccggtgt atttcaccgg attcgccccg caccggattt
     2401 cccggtaaaa aggaaagccc gataccattc aaatccggaa tgttatcggc cgctttcgga
     2461 acattcttcc cggtaaagct gcaggatacg ggaatacgcc tccccggcct gccgcccgat
     2521 tgccggcgga gaatagttat caagcgcgtg tttacgggca ttctctgcaa tcgtctggta
     2581 aaggacttca tcgtcaatca gtgatttgat cgactgatac cactcctctt cggtcgatgc
     2641 aagcaaaccg ttttcccggc ttacaataca gcggacaaac gtatcggtcg gcgacgcgac
     2701 ggtcggcacg cctacgatcg cagcctcaaa atatttgagc tcactttttg cgtgacaaaa
     2761 cgggttttcc gtatcaagcg gcgcgaggtt gataaaattc tccgcgctgt atttcagcag
     2821 ctgtgtgtat ttcatataac ccgcgtgatc gactctgcca atgagatctt cgggccagcc
     2881 ctcaagatca agataaccgg caatcctcag cctgagatcc ggatattctc tcaaaatccg
     2941 gacaacagcg ggcaaagcct gccgaaaatc gcggtcatgg gttttgcttc cactgagata
     3001 agcgagatag ttcacgtttc gttttgccat tccgccaaac tccagcccag acgcgatccc
     3061 cagctgttta tcattcagac cattgtgaat aacaaacgca cgttttcccg cctgctccat
     3121 tttttgcgta ataaacgggg tgctggccgt cgcgtagtca cactggcgaa acgttttttc
     3181 aaaattcaaa aaggtttccc gataaagccg aaccgtttcc tcgctgtgat cttcaatcgc
     3241 ctggcagaaa cgatttatat attccggcaa aaagataata tcgtcgatat cgaataccag
     3301 cggctttcgc cattttttcg cgcgccggat cagcccatcg gtatgctccg accaatcaag
     3361 cctcatgagg acaataacgt cggcccggtc gatcgcgcag tcatccaacg cgatcccgct
     3421 gtccgccact tcaaaagcgg cctcggcttt tccgcgcagc gcttcgatca gattatgaat
     3481 ccggtagcgc gccgtctgcc ccccgccctc cgcttcaaaa cggctggtta catacaagac
     3541 ccggggtttg gaattttttc ggaacatacg gaaaaccttc cgaaagccaa gccggtaaaa
     3601 cccgcctgcg gcacagtgaa gcacgtatcg gagcaggaaa gccgcctttt tgacacccag
     3661 cctgcaaact tcccggaatc cctggcgtct gaacacacta agcgcggttt taattttacg
     3721 aagaattttc atttctgctt ccccgttcca ttttttccac tgagtcgggc ggcaagtctc
     3781 tttaattgct gtttaaacgg atattgcccc tttaaaattc gtttggaaat aaaaccggga
     3841 agcatcttgg cgattcgttc ccgcatcgct tcaagccggg ctccgtaagc gatgcccatt
     3901 gtttccgtcc cctccgcggc ccgcgcaatc gcctgtgaaa tcattcggtt atcgacttcg
     3961 gcggccggag gctttccctc ttttaatatt actttctgat acagagcggc atattccgcg
     4021 cccatttccg caatggattt cggctgatag cccaaaagcc gatctctgat ttcaagcagc
     4081 cccgcccggt catccataac ttcaccgatt ttttgaagaa tttgccgttc atcagcccca
     4141 aaaggcaaaa gatacccaaa accggtctta cgaattctgc cgccgatggc gccaatgtca
     4201 aatcccagcg ccggcactcc ggc
//
LOCUS       DATALY010000384         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_427008,
            whole genome shotgun sequence.
ACCESSION   DATALY010000384 DATALY010000000
VERSION     DATALY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427008"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..808)
                     /locus_tag="VHR42_10515"
     CDS             complement(389..808)
                     /locus_tag="VHR42_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027637.1"
                     /translation="MKTKIVSLVLALTLMFTFSVPAFAKTASGPSGDIHIMLINTDDA
                     TSMVSFNGDCADCSAIIHAKTGTTKIAMTGVLKRVTDNGTTTVKSWTKTVNGETLTFD
                     KNWYVASGYDYEFEVLAKVYRNGTMEAISVTDSAHCG"
     gene            complement(894..1610)
                     /locus_tag="VHR42_10520"
     CDS             complement(894..1610)
                     /locus_tag="VHR42_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HEX3027638.1"
                     /translation="MKKTELNDRLFNTVLKAAVDENFWRELDDMPEEKELRPEYLPSP
                     ALKQKIRQAVKKGNRRAVLQKIRKAAKRAAVIAVILISVVMGSLLSVEASRNAIFNSV
                     LEWKADHVDIYFRQEEKPQPEGTGIYKPQYIPEGFVELKSEKVGLSYETTYQNEKEIS
                     IIFDQSPLSSGKTMMDTEHSKYQKITISGQKAYLLEAKTPGDKTQIIWQVDKVSLKLS
                     SKIDKKELILMAESVKYQQR"
     gene            complement(1588..2157)
                     /locus_tag="VHR42_10525"
     CDS             complement(1588..2157)
                     /locus_tag="VHR42_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3027639.1"
                     /translation="MLICIQTIQNDSERSLVEDIYRSHIGTMIYIAQGILKDRYRAED
                     AVSQAFLKIIDNLQKITWYDCNKTRGFVVIIVRNICLNMIKDEKHEKILPLELCGDVA
                     ENAQDEPLNFLINEETYNFILSCLSELCDSHKDILRLKLIYEYTDEEIADILDISQEN
                     ARVRLHRAKKALIAQMQKRGGNYEKNGTE"
     gene            2504..3163
                     /locus_tag="VHR42_10530"
     CDS             2504..3163
                     /locus_tag="VHR42_10530"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEX3027640.1"
                     /translation="MSTQISKDVSCPHCGAAVKTQMWPGISAEESPEMREKILNETLF
                     DWKCPECGYEARLVYPCLYHDKDRKFMIYVVPNGNDCALQSVDISEAFPQFRELTKRV
                     VTTPTELKEKVLIFEAGMDDLAVELVKLALTDVAEKKHGKSVSSGYFCYADEPTEKIG
                     FSFFLKGSSEPVRQDTRLDVYRKSLEIAENVGFSDGGEFTAVDSQTARNVLREYRGEE
                     D"
     gene            3166..>4167
                     /gene="asnB"
                     /locus_tag="VHR42_10535"
     CDS             3166..>4167
                     /gene="asnB"
                     /locus_tag="VHR42_10535"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX3027641.1"
                     /translation="MCGLVGFTGEVIDREAVIKTMTDKITHRGPDSSGTFANGDISMG
                     FRRLSIIDIASTGDQPIYNEDRTLVLMFNGEIYNYQDLRKTLIEKGHKFYTNTDSEVL
                     VHGFEEWHEELLNRLRGMFAFAIWNTVDKSLFIARDFFGIKPMHYTQVGNSFVYASEI
                     KSILDFPGFEKKLNKNALNNYISFEYSVPPETFFEGVFCLMPAHYLWYRKGKVETHRY
                     WEPKFEADGGMTQEQAVSEIEKVFENSVSAHKISDVEVGCFLSSGVDSSYVSTYFSGQ
                     KTFTVGFDYGEKYNEIDWAKNLSEKIKVDHHCKVISADEYWGNIRKVQYHMDQPLADA
                     "
BASE COUNT         1045 a          972 c         1017 g         1133 t
ORIGIN      
        1 gatttttggc tttgtgtaat gctttgattc tcacttgaca ctttagccgc aaacgctggg
       61 acggcgatcg caaacgaaat tgccagaaca agaaccagac tgactaactt tttaaagtta
      121 tgtttcataa tcttaattct cctttattat aagtttttat gtttgaaaac tttgacataa
      181 cacggataag cagggggatt tttcaccccg ttataagtca ttggattcat cccctttttc
      241 aagaggtttt atttcaatgc gcgttgcgca ccgtgcacgg gaacacaaca caggagtttt
      301 taggttcgcg ggccggcaga ggagtgactg ccggcccgct atgtagaaga gatgaatggg
      361 tgtaagcgat ttaaaatgag aaaacggatt atccgcagtg cgcggaatcg gttacgctga
      421 tggcttccat cgtaccgttg cggtaaacct ttgcgagcac ttcaaactca taatcgtaac
      481 cgctggcaac ataccagttt ttatcaaacg taagggtttc accgtttacc gttttcgtcc
      541 agcttttgac cgtggtggtt ccgttatcgg ttaccctttt gagaaccccg gtcatagcaa
      601 tctttgttgt gccggttttt gcatgaataa tggcggaaca atccgcgcaa tcaccattaa
      661 aactgaccat tgacgtagcg tcgtcggtat tgatgagcat aatgtggata tctccggagg
      721 gaccggatgc ggtttttgca aaagcgggaa cactgaaggt gaacatcaga gtcagtgcaa
      781 ggacaaggga aacgattttc gttttcataa gtaagacctc tcctctatat ttttgggttc
      841 tatataataa acaacgaaac cccctgtttt gttacacttt tttccaaact tttttatctt
      901 tgttgatatt tcacactttc agccatcaaa atcaattctt ttttgtcgat ttttgaactg
      961 agcttaaggc tgactttatc gacctgccat ataatctgtg ttttatcacc gggagttttt
     1021 gcctctaaca ggtacgcttt ttgtccgcta attgtaattt tctgatattt tgaatgttcg
     1081 gtatccatca tcgttttccc acttgataag ggggactgat caaaaataat ggatatttcc
     1141 ttttcgtttt gataagttgt ttcataagac aaaccaactt tttccgattt caattccaca
     1201 aacccctccg ggatatactg cggcttatag atgccggtgc cctccggctg cggcttttcc
     1261 tcctgccgga aatagatatc cacatggtcg gctttccact ccagaacgga gttgaaaatc
     1321 gcgttccggg aggcctccac gctcagcagg cttcccatga ccacagaaat caggatgacg
     1381 gcaatgaccg ccgccctttt ggcggccttc cgaatttttt ggagaaccgc ccggcggttt
     1441 ccttttttta cggcctgccg tattttctgc ttcagcgcgg gcgagggcag atactccggc
     1501 cgcagttcct tttcctccgg catatcgtcc agttcccgcc aaaagttttc gtcaaccgcc
     1561 gctttcagga cggtgttgaa aagccggtca ttcagttccg tttttttcat aattgccgcc
     1621 ccttttctgc atttgcgcaa tcagcgcctt ctttgcgcgg tgaagacgca cccgcgcatt
     1681 ttcctgggaa atatcgagaa tatccgcgat ttcctcatcg gtatattcgt aaatcagctt
     1741 cagccgcagg atatccttat ggctgtcgca cagttccgaa aggcaggaca ggatgaaatt
     1801 ataggtttct tcgttaatca ggaagttcag gggctcgtct tgcgcgttct ccgcaacgtc
     1861 cccgcagagc tccaacggga ggattttctc gtgtttctca tcttttatca tattcaggca
     1921 gatgttcctt acaataataa caacgaaacc ccttgttttg ttacagtcat accacgtaat
     1981 tttttgcaga ttgtcaatta ttttaagaaa tgcctgtgat accgcgtctt ccgcgcggta
     2041 tcggtctttt aagattccct gcgctatata aatcatcgta ccgatatgac tgcggtatat
     2101 atcttccaca agactgcgtt cggaatcgtt ttggatggtt tgaatacaaa tcagcatacc
     2161 ggctgcctcc tgatttcgac aattttctaa attataatct atatcaggaa caaaggcaat
     2221 taacaatttg ttgcttaagg tggcggaaac cgggcaaaaa tagcagtatt gtggaattct
     2281 attccttttt tctgcccgct gtgatataat cagtaaatat gttaactagg aagtgctgat
     2341 taaatcaggc gctcaaaatg ccgggccggt tttatggctg actggggaaa agcgggcgga
     2401 tacctgccgt attcggaacg ctttttgacg atgtccaaga cgcaaaaccg ggggcaaacg
     2461 cgcagcggga tttactcagc ggttccttaa ggagggaacc gaattgtcaa cgcagatcag
     2521 caaagacgtg agctgtccgc actgcggtgc cgcagtgaaa acgcagatgt ggccggggat
     2581 cagtgcagaa gagagcccgg agatgcggga gaagatcctg aacgaaaccc tgtttgactg
     2641 gaaatgcccc gaatgcggct atgaggcgcg gctggtatat ccttgccttt accatgacaa
     2701 agaccggaaa tttatgatat atgttgtacc gaacgggaac gactgcgcgc ttcagtccgt
     2761 agatatcagc gaagcatttc cgcagtttag ggagctgacc aaacgggtgg ttacaacgcc
     2821 gaccgagctg aaggaaaaag tcctgatttt cgaggccggt atggatgacc ttgcggtgga
     2881 gttggtaaag ctggctctta ccgacgttgc ggaaaaaaag cacggaaaat cggtttcctc
     2941 cggttatttc tgctatgcgg atgaaccgac ggagaagatc gggttttcct tttttctcaa
     3001 aggcagcagc gagccggtgc gccaggacac aaggctggat gtttaccgga aatcacttga
     3061 aattgcagag aacgtcggtt tttccgacgg cggtgaattt acggcggtgg attcccagac
     3121 cgcccggaac gtactgcggg agtaccgcgg cgaggaggat taaaaatgtg cggattagtc
     3181 ggatttacag gtgaagtcat cgaccgggaa gcggtcatta aaacgatgac ggataaaata
     3241 acccacaggg gaccggattc cagcggaacc tttgcgaacg gcgatatttc catggggttc
     3301 cgccgcctga gcatcatcga tatcgcttcc accggcgacc agccgattta caacgaggac
     3361 cgcacgctgg tgctcatgtt caacggcgaa atctacaact atcaggattt gcgcaaaacc
     3421 ctgattgaaa agggacataa attttatacg aacaccgatt ccgaggtgct ggttcacggt
     3481 tttgaggaat ggcacgagga acttttaaac cggctgcgcg gtatgttcgc gttcgccatc
     3541 tggaatacgg tggacaaaag cctgtttatc gcccgcgact ttttcggcat caagccgatg
     3601 cactacaccc aagtcgggaa cagctttgtt tacgcttcgg agatcaaaag cattctggac
     3661 tttccgggct ttgagaagaa gctgaataaa aatgccctca acaattatat ttcattcgaa
     3721 tactccgtac cgcccgaaac ctttttcgag ggtgttttct gcctgatgcc ggcgcactat
     3781 ttatggtacc ggaaaggcaa ggtggagacg caccgctact gggagccgaa atttgaggcg
     3841 gacggcggaa tgacgcagga gcaggcggtt tccgagatcg aaaaagtatt tgagaactcc
     3901 gtcagcgccc ataaaatcag cgacgttgaa gtaggctgct tcctttccag cggcgtggat
     3961 tccagctacg tttccacgta tttttccggc cagaagacct ttaccgtcgg ctttgactac
     4021 ggcgaaaagt acaacgagat cgactgggcg aaaaatcttt ccgaaaaaat caaggttgac
     4081 catcactgca aggtgatttc cgctgacgaa tactggggaa atatccgtaa agtgcagtac
     4141 cacatggatc agccgcttgc cgacgct
//
LOCUS       DATALY010000385         5688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_514286,
            whole genome shotgun sequence.
ACCESSION   DATALY010000385 DATALY010000000
VERSION     DATALY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514286"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VHR42_10540"
     CDS             <1..360
                     /locus_tag="VHR42_10540"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3027642.1"
                     /translation="TLGESSLSFVCEHYSHGSDEDYLSERLRAGAIKGAFVGEKLAGF
                     IGQHAEGSMGMLEVLPQYRHNHIAVGLETYLINQLLREHHTPYAQVIVGNTASLSLQK
                     KLNLSISEGLVSWMYQD"
     gene            complement(409..4704)
                     /locus_tag="VHR42_10545"
     CDS             complement(409..4704)
                     /locus_tag="VHR42_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HEX3027643.1"
                     /translation="MKLLHLADLHIGRRIGEFSMLEDQRYILDQILQIADAEQPSGVL
                     MAGDIYDKGLPPGEAVELLDDFLTGLATRQIPVFLVSGNHDSPERLNFGSRIMGKSGI
                     YIAGTFRGEVRKETLEDKFGKVNIYLLPYLKPAAARPYFETQIDTCDGAVRAALASVS
                     LDANERNILIAHQFITSGGQEPERTDSESLSLGGLDNVDASAFDGYDYVALGHLHGPQ
                     RIGRNTIRYSGSPLKYSFSEARQHKSVTVLELAEKGTLTYRLVPLVPLRNMRELKGPI
                     EELLRVGSEDPASSQDYIHATLTDEDEVYDALGKLRQVYPNLMRLDFENSRTVINAES
                     KTAASGDVAHKSPLELFSEFYQNQNNTELTEEQKNIMTDIFEQAGGQCNLKPIKIVMS
                     AFGPYSGEAVLELERFGGSGLFLITGDTGAGKTTIFDAVAFALYGEASGETRSAEMFR
                     SHFAEAETKTFVELTFIHKGKQYTVYRKPKYDRPKKNGSGTTSESADASLTLPNGDVV
                     AGYKEVTAAIVDLLGINYKQFKQIAMIAQGEFQKLLLADSKDRAEIFRRVFNTELYRA
                     GQDILKKREKEAKTLCEENGRSILQYLSGILVPDASESYRPVAELIEQKNVLVTEQII
                     ECLKRALEDDKNSWNQEKLNSAKLERAIAELIAAVTQAQYLNVSFDKLADAQKTQLEL
                     TAKSEEMSCREKELALAEKALFTVQPLEAAYLREKSQRDGLSESIRKLESDIAAKSTE
                     KEHFYALLLTEQAKEPERDQLAAEIDRLTKTLPQYEAEEVLAQEAARLINEQHRVESE
                     TDRLKQDKNRLEGLRKSLNDELERTAEAEVQLSDCRHTLELLRTTETGLRGLLQQISA
                     LKTLHKEGESLRQRFIELQKAYQESNTEYLQKEAAFFLEQAGILAENLKDGEPCPVCG
                     STDHPQRAVHTLNAPSEAELKLLKEQNEALRQGTQEASERAGSKDAEFKASAEHLRRS
                     AEELLRSKPIPDKLGGLSAAVSEELAACLVEQEKKVKEQDQLSLQAKQHKNSREQLTE
                     AEQKIRETEDLLIKANERSSSISSSLAAKNAELSVLRTTLELPSKVQALEQIKAKQQC
                     FAELKQALQQADDSYHKAQNALQSNQAVWKDQTERMATVSAQMETAFSAYTEKLVSCG
                     FSDEEEYRMSLRNEQQLQEIRTVLEEYRDCVKRIQAERLRLEEELKDKQPQNLELLLN
                     RKAELETEKTASNERIRTLWARLDANGKTLKSLQKADLERKKNEADFILISGLSKTAN
                     GELPGKQKLAFEQFVQASYFNQIIVEANKRLRYMTDNRFELLRRDNATDYRSQSGLEL
                     DVLDNYTGKVRTVKSLSGGESFKASLSLALGLSDVIQSFAGGVEVDTMFIDEGFGALD
                     AESLEQAIRTLNGLTSGNRLVGIISHVSELKERIDRQVVIQKGVTGSTLKVIV"
     gene            complement(4880..5242)
                     /locus_tag="VHR42_10550"
     CDS             complement(4880..5242)
                     /locus_tag="VHR42_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3027644.1"
                     /translation="MEFCWSTLQVKNMEESLSFYQGILSLPLNRRFPAGPNMEIAFLG
                     DGDTKIELVCSKDKENINIGQDISWGFAVNSLDETMKHLQENGVTSFNGPFQPNPHVK
                     FIYVIDPNGMKIQLVENM"
     gene            complement(5470..>5688)
                     /locus_tag="VHR42_10555"
     CDS             complement(5470..>5688)
                     /locus_tag="VHR42_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HEX3027645.1"
                     /translation="RLEDNIPDIIRILSELPKPERLVQMLSTAGCAVTLKDIDLPEEI
                     REPSIRLSPYVRNRLTFMRLLKLFELER"
BASE COUNT         1318 a         1468 c         1286 g         1616 t
ORIGIN      
        1 actctgggtg aaagctccct ttcatttgtt tgcgagcatt actcccacgg gtctgacgag
       61 gattatcttt ccgaacggct tcgggcagga gcaataaagg gcgcttttgt aggtgaaaaa
      121 ttggccggtt ttatcggcca acacgcggaa ggctcgatgg ggatgctgga ggttttgccg
      181 caataccgac ataatcacat tgcggtcggt ttggaaacgt atctcatcaa tcagctccta
      241 cgcgagcacc atactcctta tgctcaggta attgtaggga atacggcatc gctgagcctg
      301 cagaaaaaac taaatttgtc catttcagaa ggtcttgtga gttggatgta tcaagactaa
      361 tgacaagatt atagcaaagg cagcgcttaa aatgagcgct gccttttatt atactataac
      421 tttcagcgtg ctgccggtaa cacccttctg tatcacaacc tgacggtcaa tgcgttcctt
      481 cagctcgctg acgtgagaaa tgattccgac cagccggttg ccggaagtaa ggccattcaa
      541 cgtacggatt gcctgttcaa gcgattccgc atccaacgct ccgaaccctt catcgataaa
      601 catggtgtcg acctccacac ccccggcaaa gctttggatt acatcggaaa gaccaagggc
      661 gagcgaaagg gaagctttaa atgattctcc gcctgagagt gatttcaccg tacgcacctt
      721 tccggtgtaa ttgtcaagca catcaagctc taaaccggac tgtgaacggt aatccgtcgc
      781 gttgtcgcgc cggagcagtt caaacctgtt gtcggtcata tagcgaaggc gtttattggc
      841 ctcaacaatg atttgattga agtaggacgc ctgcacaaac tgttcaaacg caagcttctg
      901 ctttccgggc agttctccgt ttgccgtctt tgacagcccg ctgattaaga tgaaatccgc
      961 ttcgtttttc tttcgctcca gatccgcctt ttgcagtgat ttgagagttt ttccgtttgc
     1021 atccaggcga gcccaaagag tgcgtattcg ttcattcgaa gcagtttttt ccgtttcgag
     1081 ctctgctttt cggtttagga gcagctctaa attctgcggt tgcttatctt ttaattcttc
     1141 ctccaaccgt aaccgctccg cctgaatccg tttcacgcag tctcggtatt cctcaaggac
     1201 ggtgcgaatt tcttgcaact gctgttcatt gcggagcgac atacggtatt cttcctcatc
     1261 gctgaatccg caggaaacaa gcttttccgt gtaagcagag aaggccgtct ccatttgtgc
     1321 cgacacggtg gccatgcgtt cggtttgatc cttccaaacg gcctgattgc tttgaagcgc
     1381 attttgggcc ttgtgataag aatcatcagc ctgctgcaaa gcctgcttca attcggcaaa
     1441 gcactgctgc tttgctttaa tctgctcaag cgcctgtact tttgagggaa gttcgagtgt
     1501 ggtgcggagc acggaaagct ccgcgttttt tgcagcaagc gaggaagaaa tggaactact
     1561 ccgctcgttc gctttgataa gtaaatcttc ggtttccctg attttttgtt cggcctccgt
     1621 cagctgttca cgggaatttt tatgctgttt ggcctgcaga gaaagctggt cctgttcttt
     1681 gaccttcttt tcctgctcca ccaagcatgc cgcaagttcc tcgctgactg ccgcagacag
     1741 cccaccaagt ttgtcgggaa tcggcttcga ccgaagcagt tcttcggcag accttcgcaa
     1801 atgctcggcg gaagctttga actcggcgtc cttactccct gcccgttcgc ttgcctcctg
     1861 cgtaccttga cgaagcgctt cattttgttc tttcagcagc ttcaattccg cttcgctcgg
     1921 cgcattcagc gtatgaaccg ctctttgcgg atgatcggtg gaaccgcaga ccggacatgg
     1981 ttcaccgtct tttaggtttt cagccaaaat tcccgcctgc tcaaggaaaa acgcggcttc
     2041 tttctgaaga tattctgtat ttgactcctg atacgctttc tgcagctcta taaaacgctg
     2101 ccggagactt tccccttctt tatgtaaggt ctttaatgct gaaatctgct ggagaagccc
     2161 acgcagcccg gtttctgttg tgcggagcag ttccagtgtg tgacggcagt cggaaagctg
     2221 cacttccgct tcggcagtgc gttccaactc gtcgttaagg cttttccgca gaccttccag
     2281 acgatttttg tcctgtttca gcctgtctgt ttcactctca acccggtgct gctcatttat
     2341 aaggcgagcg gcttcctgag caagcacttc ctccgcttca tattgcggaa gtgttttcgt
     2401 cagacggtca atctccgctg caagctgatc gcgctctggt tccttggcct gttcagtcag
     2461 aagtaacgca taaaaatgct ctttttcagt actctttgcg gcaatgtcac tttccaattt
     2521 acgaatactt tcggataaac cgtcccgctg ggatttttca cgaagataag ctgcttccaa
     2581 aggctgaacc gtgaacaaag ctttttcggc caaagctagt tctttttcac ggcaggacat
     2641 ctcctctgac tttgctgtca gctccaattg tgttttctgt gcgtcggcca gcttatcgaa
     2701 ggaaacgttg aggtactgcg cctgcgttac tgcggcaatt agttccgcaa tagcacgttc
     2761 cagtttagcg gaattcagtt tttcctgatt ccagctgttt ttgtcgtctt ccaaagcccg
     2821 tttcaggcat tcgatgatct gctcggtcac caatacattt ttttgctcga taagctcggc
     2881 aaccggacgg taggattccg aagcatccgg aaccaatata ccggacagat attgaagaat
     2941 gctccgcccg ttttcttcgc agagcgtctt tgcttctttt tcccgctttt ttaaaatatc
     3001 ctgtcccgcg cggtacagtt ccgtattgaa aacgcggcga aaaatttcag cgcggtcttt
     3061 gctgtctgcc agcagcagct tttgaaattc accctgtgca atcatcgcga tctgtttgaa
     3121 ctgcttgtaa ttgataccga gcagatcaac gattgcggcg gtcacttcct tatacccggc
     3181 aaccacatct ccgttcggca gagtcaatga cgcgtcagcc gattccgatg tagttccgct
     3241 gccgttcttc ttcggacggt cgtatttggg ctttcggtaa acggtatatt gcttcccctt
     3301 atgtataaac gtgagctcta cgaatgtttt ggtttccgct tccgcaaaat gactccgaaa
     3361 catttccgca gaacgcgttt cgccgctcgc ttccccgtac agcgcaaagg caacagcatc
     3421 aaaaatggtt gttttgccgg caccggtatc gcctgtgata aggaacaagc cgctgccgcc
     3481 gaagcgttcc aactcgagaa ccgcctcgcc ggaataaggg ccgaatgcgc tcatgactat
     3541 ttttatcggt ttcaagttgc attgcccccc cgcctgttca aagatatcgg tcatgatgtt
     3601 tttctgttct tccgtcagtt ccgtgttgtt ctgattttga tagaactcag aaaacagctc
     3661 cagcggactt ttatgagcaa catcaccgga cgctgctgtt ttcgattcag cattgattac
     3721 cgtacggctg ttctcaaagt ccagacgcat cagattcggg tatacctgac gcaacttgcc
     3781 caaagcgtca taaacttcat cctcgtccgt tagggtcgcg tggatataat cctgcgaaga
     3841 tgccgggtcc tcacttccga ctcgaagcag ctcttcaata gggcctttca gttcgcgcat
     3901 gttgcgcagg ggcacaaggg gcaccaatcg ataggtaagg gttccttttt ccgctaattc
     3961 aagcaccgta accgacttgt gctggcgtgc ctcggaaaag gaatatttca ggggtgaacc
     4021 ggagtaacga atcgtattgc ggccgatacg ctgcggcccg tgcaggtgac cgagcgccac
     4081 atagtcatac ccgtcaaaag cggaagcatc cacattatcc agcccgccga gggacaggct
     4141 ttccgaatcg gtacgttccg gctcctgccc tccacttgtt atgaactgat gtgcaatcag
     4201 aatattccgt tcattcgcgt cgagggaaac ggacgcaagc gccgcgcgca ccgcaccgtc
     4261 gcaagtatca atctgggtct caaaatatgg tcgtgccgcg gccggcttta aatacggaag
     4321 caaatagatg tttaccttgc cgaacttgtc ctccaacgtt tccttacgca cttcgccgcg
     4381 gaaggttccc gcaatataaa tcccggattt ccccataatt cggctgccaa aattcagacg
     4441 ctccggtgaa tcgtgattcc cgctgactaa aaataccgga atttggcgcg tggcaagccc
     4501 ggttaaaaaa tcatcgagca actctaccgc ctccccggga gggagacctt tatcgtagat
     4561 atctcctgcc attaaaactc ccgaaggctg ttccgcatcg gcaatctgca aaatctggtc
     4621 taatatgtat cgctgatcct ccagcatact gaactcaccg atgcgcctgc cgatatgcaa
     4681 atctgctaaa tggagtaatt tcataacatc ctcacttcct ttactttttc tatatgaaat
     4741 ataaaaacct attcactcaa tatcttattt tactgaaaaa agccgtgaaa gaaaagggta
     4801 tttaatcatt agttggcata agtaagtaat tttcttctgc cataatgaaa aaagcgcctc
     4861 cgaagaagcg ctctttctgt tacatgtttt cgaccaactg tattttcatt ccattcgggt
     4921 caatcacata gataaattta acatgtgggt tcggttgaaa cgggccattg aagctcgtca
     4981 cgccattttc ttgaaggtgt ttcattgttt catccagtga atttaccgca aatccccacg
     5041 aaatatcctg cccgatgtta atattttctt tgtctttgct gcagaccaat tcgatttttg
     5101 tatcaccgtc gccgagaaat gcgatttcca tattcggccc tgccggaaag cgcctgttca
     5161 gcggcaaact gagtattccc tgataaaagc ttagtgactc ctccatattt ttaacctgta
     5221 atgtgctcca gcaaaattcc atttattttt cctccttaaa ttctttccgt tccagttccg
     5281 caaattcaga aataaacata tctgctaggc ctagtttgta aaatgattga agttgtgcaa
     5341 aaagataagt cagccggact tctatgctga aacgtatcac aaagataata cagagaaaga
     5401 aaaaccggaa taaaatcttt catttatggg ttttattccg tcgtttttct ttttacaaac
     5461 aaggcctaat taccgttcta attcaaacaa tttcagcaga cgcataaacg tgagccggtt
     5521 gcggacatag ggtgacagac ggatggacgg ttcacgaatt tcttccggta aatcgatgtc
     5581 ttttaaagta actgcgcagc ctgccgtgga aagcatttga acgagtcttt ccggcttcgg
     5641 aagttcggat agtatgcgga taatatccgg tatgttatcc tccagccg
//
LOCUS       DATALY010000386         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_84738,
            whole genome shotgun sequence.
ACCESSION   DATALY010000386 DATALY010000000
VERSION     DATALY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84738"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..226
                     /locus_tag="VHR42_10560"
     CDS             32..226
                     /locus_tag="VHR42_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027646.1"
                     /translation="MPEKMEKRIVSAGNAEYASSSDEKEPENAGQPQNSGEKNDQQNG
                     TPQTPELDPELSLGRHGPAQ"
     gene            226..558
                     /locus_tag="VHR42_10565"
     CDS             226..558
                     /locus_tag="VHR42_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027647.1"
                     /translation="MSLHDQIEEDLSIFLDLDEMAAKHTVCLPGDPAPREIAAVVDND
                     EGQQNALRSQSGVYSGTVLFYAKTSDVSGITANSMIQFDDRPYQVAGIVEEDGMTQVT
                     LQAGEGGF"
     gene            558..1235
                     /locus_tag="VHR42_10570"
     CDS             558..1235
                     /locus_tag="VHR42_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027648.1"
                     /translation="MANNVLYANTKQLDKVADLLKNYPEEATKIMNRVLVRSESAVKS
                     AVKKEIPKVYAITSPKINDALNGKKRKIKTIMGAAGEGSISIEVLGRPLTLSQFRATP
                     KSPPEKTKSGKRRKFHVRAMFLKEKGMLPVGPVHGKDGKLKSVFLMPTTSGSEQYLFA
                     YRTGIKNGQKEKLKVVRSVSVPQMVMNEKVGPAIIDKVNQTVFDRLTHELDWSFGNLG
                     TNLAGGK"
     gene            1238..>1608
                     /locus_tag="VHR42_10575"
     CDS             1238..>1608
                     /locus_tag="VHR42_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027649.1"
                     /translation="MQTVSSLEQIKKWLEDLITAEKITFKSPQKDNKADTYKLVPPAV
                     HVGFVPPNSIVDQNSKIRIPCLVVGTSETDGDWENTMMNLQITAAVYDPGSQTMASDR
                     QQLQLIPNFDGYVTLLNFLDRV"
BASE COUNT          490 a          329 c          409 g          380 t
ORIGIN      
        1 acatatcatt ccggccgggc ggacgatcgc tttgccggaa aagatggaaa aacgtatcgt
       61 ttctgccggg aacgccgaat atgcttcttc ttcggatgag aaagaacctg aaaatgcagg
      121 acaaccgcaa aattccggag agaaaaacga tcagcagaac ggtacgccgc aaaccccgga
      181 actggatccg gaacttagct taggccgtca tgggccagcg cagtaatgtc gctgcatgat
      241 cagattgagg aagatctttc gatctttctt gatctggatg aaatggccgc caaacatacc
      301 gtatgccttc ctggagatcc cgcaccgcgg gaaattgctg ctgtggttga taacgacgaa
      361 gggcagcaga atgctctgag atctcagagc ggcgtctatt ccggaactgt tctgttttat
      421 gcaaaaactt cggatgtttc cggaatcacc gcaaattcca tgattcagtt tgatgaccgg
      481 ccgtatcagg ttgccgggat tgttgaagaa gacggcatga cgcaggttac gctccaggct
      541 ggagagggtg ggttttgatg gcgaacaatg ttctttatgc gaacacaaag cagttggata
      601 aagtcgccga tttgctaaaa aactatccag aagaggcgac caaaatcatg aatcgagtac
      661 tggtacggtc agaaagtgcc gtaaaatcag cagttaaaaa ggaaattcct aaagtatacg
      721 ctattacaag ccctaaaatt aatgatgctt taaatggtaa aaaacgtaaa ataaaaacta
      781 ttatgggtgc ggcgggagaa ggcagtattt caattgaggt tttaggacga ccgcttacgt
      841 tatcacagtt tcgcgcaacg ccaaaatctc ctccggagaa gaccaaaagc gggaaaagac
      901 gcaaattcca tgtgagggca atgttcttga aggaaaaagg tatgttaccg gttggaccgg
      961 tacatggaaa agacggtaag ttaaaatcag tctttctaat gccgacgacg agtggttcgg
     1021 aacaatatct atttgcatat cgcactggta ttaagaacgg tcaaaaagaa aaattaaaag
     1081 tagtacgttc tgtgtccgtt ccgcaaatgg tgatgaatga aaaagtaggg cctgcaataa
     1141 ttgataaggt aaatcaaacc gtttttgatc gattaacaca tgaattagat tggtcgttcg
     1201 ggaacctggg tacaaatctt gcgggaggaa agtaacgatg cagacagttt catcgcttga
     1261 acagatcaaa aaatggctgg aagaccttat cactgctgaa aagattacat tcaagtctcc
     1321 tcagaaagac aataaggcgg atacatacaa gttggtccca ccggccgttc atgtgggatt
     1381 tgttccgccg aacagcattg tggatcagaa ttcaaaaatc aggattccat gcctcgttgt
     1441 gggaacgtcc gaaacggacg gtgattggga aaatacaatg atgaatctgc agatcacggc
     1501 agctgtttac gacccgggca gccagacgat ggcgtccgat cggcagcagc tccagctgat
     1561 cccgaatttc gatggctatg ttacactgct gaatttcctt gatcgtgt
//
LOCUS       DATALY010000387         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_858013,
            whole genome shotgun sequence.
ACCESSION   DATALY010000387 DATALY010000000
VERSION     DATALY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858013"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VHR42_10580"
     CDS             <1..453
                     /locus_tag="VHR42_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HEX3027650.1"
                     /translation="DICVEGEDESASLSLTLNTLHMIAAKQRSLRFIKAAFEMRCAVG
                     AGYTPMLDGGCGVCGRTDARNLDCAGGCLICDSCALKPEHKGIAVGLHPDVIRAIRYI
                     AEAAPSRVFAFRLTEEAELNFFDVCENYLTAQIDRRFPTLEYFNALYG"
     gene            499..2889
                     /locus_tag="VHR42_10585"
     CDS             499..2889
                     /locus_tag="VHR42_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX3027651.1"
                     /translation="MPDFERAQITLEYNKILAELAGCAHTEGAAEMALQLTPTHDIDR
                     VRQLQAQTGDAKKLASLKGTPSFGSVKDVNPALDRADRGAILTMRELLDIAGVLRTAR
                     LLIDYINSDKNYETVLDEHFKRLTVNRPLEERIGRTIIAEDMIADEASPALADIRRKM
                     RNASNRVKDILQRYVSGDFSKYLQENIITQRSGRYVIPVKNECKNEIKGLVHDTSASG
                     ATVFIEPMGVVEANNELRELESSETHEIDRILAELSAACAGAADGISLNYYNITALAF
                     IFARAELSFRQNAAQPRISAGYSVELFHARHPLLDAKTVVPINITLGGEFDTLVITGP
                     NTGGKTVSLKTLGLFALMTQAGLHIPADDTSTMPVFTDILADIGDEQSIEQSLSTFSA
                     HMVNIVSILREAKKGSLVLFDELGAGTDPVEGAALAVAILESVRERGALCAATTHFAE
                     LKEFALNTEGVSNASCEFDVETLRPTYRLIIGTPGRSNAFAISTKLGLDDSIVERART
                     KMSGSSRRFEDVIEKLEATRIEMETQRDAAVKMRRDYEAHTAEAEQALKMKIAETERE
                     LEKQRRKAAQMLESAKVSSEFVFGQLSELKRQRESERFADELETARDKVRRTLRETDD
                     IVNPVREKVTQNYRPPRPFAKGDDVLLVNINKTGILLDSPDGNGDVTVQAGIIKMKTN
                     VKNLMLADGERVTFTDRDKKKVSATSYRTSVRADFKNELDLRGMNGEDAWAAVDKYLD
                     EAKLVNLQSVRLIHGKGTGALRTALWKLIKGDPRIASFRMGQYGEGDLGVTVVELK"
BASE COUNT          814 a          765 c          823 g          622 t
ORIGIN      
        1 gatatctgcg ttgagggcga agatgaaagc gcatcgctca gcctgacgct taacacactt
       61 cacatgattg cagcgaaaca gcggagtcta cggtttatca aagcggcgtt cgagatgcgg
      121 tgcgctgtcg gggcgggtta tactccgatg cttgacggcg gatgcggggt atgcggcaga
      181 acggatgccc ggaatcttga ctgcgcaggc ggctgcctga tctgcgattc atgcgcctta
      241 aaaccggagc ataagggtat tgcggtcgga ctgcatcccg acgttatccg ggccatacgg
      301 tatatagccg aagccgcgcc gtcaagggta ttcgcgttcc ggctgaccga agaggcggaa
      361 ttgaattttt ttgacgtctg cgagaattat ttgacggcgc agattgaccg caggtttcca
      421 acactcgaat attttaacgc gttatacgga taataagaaa catataaaca gacaaagata
      481 agcagggatt taaatatcat gcctgatttt gaaagagcgc agataacgct cgaatataat
      541 aaaatactcg ccgaacttgc cgggtgtgcc cataccgaag gcgcggcgga aatggcgttg
      601 cagctcacgc cgacgcacga cattgaccgc gtgcggcagc tgcaggcgca gacaggcgat
      661 gccaaaaagc ttgcctcatt gaaaggcaca ccctcattcg gctccgtcaa agatgtaaat
      721 ccggcacttg accgggcgga ccgcggagcg attcttacga tgcgcgaact gctcgatata
      781 gccggagtgc tccgcaccgc gcggttactt atcgattata taaattccga caaaaactac
      841 gaaacggtgc ttgacgaaca ttttaaaagg cttacggtca accgtccgct cgaggagcgg
      901 atcgggcgca ctataatcgc cgaagatatg atagcggacg aagcaagtcc cgctctcgca
      961 gatatccggc ggaaaatgcg caacgcgtca aaccgcgtca aagatatttt acagcggtat
     1021 gtatcgggtg acttttccaa atacctgcag gagaatatca tcacgcagcg cagcggaaga
     1081 tatgtgatcc cggtcaaaaa cgaatgtaaa aacgagataa agggtctcgt ccacgacaca
     1141 tcagcttcgg gagccaccgt attcatcgag ccgatgggtg ttgtcgaagc taataatgag
     1201 ctgcgcgagc ttgaaagcag cgaaactcat gaaatcgacc gcattttagc cgaactgtca
     1261 gccgcttgtg ccggggctgc ggacgggatt tcgttaaatt attacaatat tacagcgctt
     1321 gcgtttatat ttgcgcgggc ggaattatcg ttccgccaaa acgcagcgca gccgcgcatc
     1381 tcggccggat acagcgtcga gctgtttcat gcgcgtcatc cgctgctgga tgccaaaact
     1441 gtggttccga taaacataac gctgggaggc gagtttgata cgttggtcat aaccggcccg
     1501 aacaccggcg ggaagaccgt ttcgttaaaa actctcggcc tgtttgcgct gatgacccag
     1561 gcggggcttc acattcccgc cgacgacacc tcaacaatgc cggtatttac cgatattctg
     1621 gccgatatag gcgacgagca gtcaatcgag cagtcgctgt ccaccttctc ggcgcatatg
     1681 gtaaatatcg taagtatact gcgtgaagcg aaaaaaggca gtcttgtgct gttcgacgag
     1741 cttggcgcgg gtacagatcc ggtcgagggc gcggctctcg cagtcgcgat acttgaaagc
     1801 gtgcgcgaac gcggcgcact ctgcgcggct accactcact tcgccgagct gaaagaattc
     1861 gcgctgaata ccgaaggcgt gtcaaacgca tcctgcgagt tcgacgtgga aacattaagg
     1921 ccgacttacc gcctgattat cggcacaccc gggagatcaa atgcattcgc tatttctaca
     1981 aaactcggac ttgacgattc gattgtcgag cgcgccagga caaagatgtc cggcagcagc
     2041 aggcgttttg aggatgtcat cgaaaaactt gaagcgacgc gcatcgaaat ggaaacccag
     2101 cgcgacgccg cggttaaaat gcggcgtgat tatgaggctc ataccgccga agccgaacag
     2161 gcattaaaaa tgaaaatcgc ggaaaccgaa cgtgagcttg agaagcagcg ccggaaggcg
     2221 gcccagatgc tggaaagcgc caaggtttca agcgagtttg tattcggaca gctctccgaa
     2281 ctgaaacgtc agcgcgagtc tgagcggttc gctgacgaac ttgagacagc acgcgataaa
     2341 gtacgccgta cattgcgcga aaccgatgat atcgtcaatc ccgtccgcga aaaagtgact
     2401 caaaactacc gtccgccgcg tccgttcgcc aaaggcgacg acgttctgct cgttaacatc
     2461 aacaaaaccg gcatattgct cgattctccg gacggaaacg gtgacgtaac cgttcaggcg
     2521 ggtattatca agatgaaaac caacgtaaag aacctcatgc tggctgatgg tgagcgcgtt
     2581 acttttacag accgcgacaa aaagaaagtt tccgcgacaa gctaccgtac ttctgtacgc
     2641 gccgacttca aaaacgaact tgatctgcgc ggcatgaacg gcgaggatgc ctgggcggcg
     2701 gttgacaaat accttgacga ggcgaagttg gtcaacctgc agagcgtccg tcttattcac
     2761 ggcaagggca caggcgcgct caggaccgcg ctgtggaaac tgattaaggg agacccgcgt
     2821 atcgcttctt tccgaatggg tcaatacgga gaaggggacc tcggtgttac ggtggtcgaa
     2881 ttaaaataaa tttttaaata taaacagtaa aataaaaaac aagaaggaga aaattatgcc
     2941 aaaccttgat ttaaacctcg gcgcacagct ttacacgctc cgcgattaca ccaaaaatgc
     3001 cgtcgacttc acagaaacaa tgaa
//
LOCUS       DATALY010000388        11207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_343701,
            whole genome shotgun sequence.
ACCESSION   DATALY010000388 DATALY010000000
VERSION     DATALY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343701"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..91
                     /locus_tag="VHR42_10590"
     CDS             <1..91
                     /locus_tag="VHR42_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027652.1"
                     /translation="NGAVWLDPEKTTPYEFYQYWRNVDDADVP"
     gene            complement(68..490)
                     /gene="tyrS"
                     /locus_tag="VHR42_10595"
                     /pseudo
     CDS             complement(68..490)
                     /gene="tyrS"
                     /locus_tag="VHR42_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
     gene            complement(520..954)
                     /locus_tag="VHR42_10600"
     CDS             complement(520..954)
                     /locus_tag="VHR42_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027653.1"
                     /translation="MISANPGSPPRPLAKIASGGEISRVMLAIKKVLAGSDDIDTLIF
                     DEVDTGVSGKAAQKIGMSLRQVSKTRQVVCVTHLAQIAAQADRHILIEKHIRGGNTYT
                     SLRALDFDGRKKELARIMGGVDITELTLRNAEEMLRLSGISN"
     gene            complement(1016..2221)
                     /locus_tag="VHR42_10605"
     CDS             complement(1016..2221)
                     /locus_tag="VHR42_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3027654.1"
                     /translation="MLSQLHIENVAVIENADLNLKRGLNVLTGETGAGKSILIDSINL
                     ALGERVTKDIVRTGSRKALVGALFTEISEEAEKLVRELGMECEEDGSLLIQREISADG
                     RGSCRISGRPATVSMLREIGRKLVNIHGQHDNQALLSPDKHIFYLDRFSQTHELLSRY
                     EEAYRRVKQIERELSGILTDETARVRRIELLSYQINEIESAELKPGEEEDLAAQKTVI
                     VNAEKITQSVNRAYTLLSGDNETAENTDSAERGARELVSTAGECLAAAAEYNTDYKSL
                     AQRITDLSYEIEDCVSELRSGMNETEYNQEDLDWIEQRLDMIYRLKLKYGGSVPEIMS
                     YLENAKKELDGMESSDEKAQKLKKMLDQEKNKLTENAGALSRARHHACKSMERGVKSE
                     LEFLDMPRR"
     gene            complement(2253..2702)
                     /gene="argR"
                     /locus_tag="VHR42_10610"
     CDS             complement(2253..2702)
                     /gene="argR"
                     /locus_tag="VHR42_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX3027655.1"
                     /translation="MKQSRHKKIIELIKEHDIGTQEMLLEMLRKNGFDVTQATVSRDI
                     KELRLVKTLSKNGEYIYSTGKMPSNDLSAKFDFLLNESVIKVDYVMNQVIIKCYAGHG
                     NALCEIMDVMHFEKIVGSLAGDNTIFVIMRNEQDAKELYDFFIEKLS"
     gene            complement(2707..3558)
                     /locus_tag="VHR42_10615"
     CDS             complement(2707..3558)
                     /locus_tag="VHR42_10615"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX3027656.1"
                     /translation="MKIAVIANEERDLNYCCTKAICSRLVSLGAQVLVSREVPGFEDS
                     PVRKLEKEEIYQQAGIIVALGGDGTILHIAKKAAQTNIPVLGVNVGRLGYMAGIEAYE
                     LESLSKLIGRDYQIDHRMLLRIEITGARGKKVFHALNDAVVSKGALSRIIDIEVTCNG
                     RRVSGYRADGVIVSTPTGSTAYALSAGGPVIDPVLESIGVIPICPHSLISRSLLFSPA
                     SKIGIVTGKANEKEVFLTVDGQETIQLSDDDQVSITRADHKAKLIRLKDNTFYEVVNQ
                     KLSERGI"
     gene            complement(3555..4373)
                     /locus_tag="VHR42_10620"
     CDS             complement(3555..4373)
                     /locus_tag="VHR42_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX3027657.1"
                     /translation="MSRKRRIDVLLVEKGLEQSRERAKALIMSGLVFADNQRVDKPGD
                     EINPDSQIEIRGNTLRYVSRGGLKLEKAMDAFGLEMNGKVCMDIGASTGGFTDCMLQN
                     GASKVYSVDVGYGQLAWKLRNDPKVVNFERTNIRYLTDEQVGEQVDFFSVDVSFISLT
                     LVLPVAYRFLKNNGEAVCLIKPQFEAGREKVGKKGVVRDPRVHVEVIEKVCNFAAKLG
                     FVVEGITFSPVKGPEGNIEYLLYLKKTCQTHENLFKIEDVVILSHKELDGGEAH"
     gene            complement(4351..6240)
                     /gene="dxs"
                     /locus_tag="VHR42_10625"
     CDS             complement(4351..6240)
                     /gene="dxs"
                     /locus_tag="VHR42_10625"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX3027658.1"
                     /translation="MDKNRLLDRINSPEDLKYLSKKQLNIMCEEIRNELIDTVSNTGG
                     HLSSNLGVVELTVALHRVFNSPEDQIVWDVGHQCYTHKLLTGRRNRFETIRQYGGLSG
                     YTKTYESAHDPFGAGHSSTSISAAYGMAKAKTLKKEDGYVIAVIGDGALTGGLAYEGL
                     NNAGRSHDNLIVILNDNKMSISKNVGAIARHLAVIRAKPGYLKTKDFIEYILNHTGII
                     GKKLRKIMVASKSAIKNTLYHSTIFEDMGFVYLGPGDGHNIEEICRLLERAKTINRPC
                     LIHLMTVKGKGYTFAEADPRTFHGVSQFDVETGDLVAGSGRASFSDVFGKTLIRLAEK
                     DESICAITAAMAAGTGLTDFAYTYRQRFFDVGIAEEHAVVFAAGLARNGMIPVFAVYS
                     TFLQRAYDQILHDAALQDLKVVFAIDRAGLVGEDGETHQGVFDAAFLNTIPGMVVYSP
                     TTNDELQNFLYAACYETEGPACVRYPRGCEPMLPADFHPSFGSFDLYGSGSADILLIT
                     YGRLFAYASAAADELKRTGRNVSVLKLNRIKPIDQDCFEITSYFKSVYFFEEGIRSGG
                     VGENFLDGLNKKGFQGSSKIYAIDDQFVAQGNVDKLLSILKLDTQGMVEAVLRECENE
                     PKKKN"
     gene            complement(6282..6728)
                     /locus_tag="VHR42_10630"
     CDS             complement(6282..6728)
                     /locus_tag="VHR42_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEX3027659.1"
                     /translation="MRVLYLILKNYILVSAVVSWMLSQVIKTILTLISTKRFDTERIF
                     GAGGMPSAHSAMVCALLMGIARKCGPSSPEFALAVAFAAVVMYDAMGVRRAAGEQAKI
                     LNKLVDKWEEDGTEITDKDLKEHLGHKPVEVLAGALLGILVSLAMA"
     gene            complement(6748..7647)
                     /locus_tag="VHR42_10635"
     CDS             complement(6748..7647)
                     /locus_tag="VHR42_10635"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX3027660.1"
                     /translation="MNNNLVEQRINEYTDMVNKALDRYLPDTSCPQKDVCEAMRYSAM
                     AGGKRIRPTLLLEFCKVAGGDAEATLPFACAVEMIHTYSLIHDDLPCMDNDDLRRGKP
                     SCHIRFGEATALLAGDALLSLAFETALCNNYKGKIKPENALRAVYELARASGAEGMVG
                     GQIIDLESEGHQVSLETLKFMHNKKTGAMITASARMGCILADAGENEIQWAAGYADNL
                     GLAFQIVDDILDATSDNETLGKPVGSDEDNDKSTYITHLGIEKSKVLVQKLTDEAVLS
                     LKGFGPKSEFLSLLAEALANRDR"
     gene            complement(7637..7858)
                     /gene="xseB"
                     /locus_tag="VHR42_10640"
     CDS             complement(7637..7858)
                     /gene="xseB"
                     /locus_tag="VHR42_10640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX3027661.1"
                     /translation="MKKEMTFESAISRLEKIVELLEQGDVTLDESMKLFEEGAHLSVL
                     CSRKLTEAEQKIIKINDSEKENGEANDEQ"
     gene            complement(7886..9133)
                     /gene="xseA"
                     /locus_tag="VHR42_10645"
     CDS             complement(7886..9133)
                     /gene="xseA"
                     /locus_tag="VHR42_10645"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX3027662.1"
                     /translation="MNNGFIILSVSQLNRYVKSLLESDPNLINLFVRGEISNFTNHYR
                     SGHLYMTLKDESAVIKAVMFKSSAQRLQFLPQNGMKVIAMGRASLYDRDGQFQFYIND
                     LQPDGVGALHTAYEQLKERLGREGLFDEKNKKPIPEYPERIGIITSPTGAALQDMLQI
                     LNRRWPVAEILLCPVLVQGEEAPRQICRAIRYFNERQPVDVLITGRGGGSIEELWAFN
                     DETVARTIVDSRIPVISAVGHETDYTIADFAADLRAPTPSAAAELVSPDLAAVAEYVR
                     SLEFSIEKATKNKINDRKVRLRDVTSTRALKEPLYNVEFRRMKLDRLTADLAGAARAE
                     VSLQNACFRELAAKLDALSPLKVLSRGYSIAYDRRGDILSSVKGLLPDDEIDLRLSDG
                     TAKCKVSGIEALGKKRKKPVAAK"
     gene            complement(9130..10068)
                     /locus_tag="VHR42_10650"
     CDS             complement(9130..10068)
                     /locus_tag="VHR42_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M22"
                     /protein_id="HEX3027663.1"
                     /translation="MGFYLGIDTSNYTTSAAVYDGETGAIYQQKKLLWVKKGEKGLKQ
                     SDAVFQHVRQLPEMIGKLMDGSGFAVKAVGASIRPRDAEGSYMPCFTVGECVARSVAS
                     AAGIAFYDFSHQAGHIVAALFSAGRLEMLKEKFIAFHVSGGTTEAVLYCPGNPLEFNI
                     SLIAKSLDLKAGQLIDRTGLLLGLDFPAGPALEKLALQSSREFKIKPCMKGPDCCLSG
                     IENQCADMKRRGEPDEDIAKFCMDSVEAVIEAMTQGLLRDHPGSRLLYAGGVMSNRSI
                     REKMTGKYGAVFAEPAFSSDNAAGIAVLTKLRADYS"
     gene            complement(10073..10546)
                     /gene="nusB"
                     /locus_tag="VHR42_10655"
     CDS             complement(10073..10546)
                     /gene="nusB"
                     /locus_tag="VHR42_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX3027664.1"
                     /translation="MNRREAREQALCLVFERLFNNEPLPEIIELAIESRELTPDPFAK
                     ELAAGVIEHLDEIDGTIEKYSIGWSKSRLSKVVLSILRLAIFEISYKSEIPISVSINE
                     AVELSKKYAGNGDGAFINGMLGAFVRSEKGEDPEKAKNIEKSEKTEKSETSEEAE"
     gene            complement(10796..11167)
                     /locus_tag="VHR42_10660"
     CDS             complement(10796..11167)
                     /locus_tag="VHR42_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX3027665.1"
                     /translation="MEQNHYADAGTLKISEDVLATVAGMTAREIKGVASLSLRPGPEP
                     RGFMKNRSLGKTIRLELKDGEAVIDIYVNLFIGFKIPDVASEIQDKVKESIQNMTGIT
                     VSKVNVHVSGVVIDQGAGGKQ"
BASE COUNT         2655 a         3024 c         2446 g         3082 t
ORIGIN      
        1 gaacggcgcc gtctggcttg atcctgaaaa aacaacgccc tatgagtttt atcagtattg
       61 gcgtaatgta gatgacgccg atgtccctta aaaagtcaat ataattcagg ttcaacagcc
      121 agtcggcatt attaaccata agcgctttcc cgtcagaaaa gtcgataaag caggacatct
      181 gcttccggaa acagccgata ttgtggctga ttgtttcccg cgtcatcatt ttcctcatgt
      241 cggtacgtcc gctggggtcg ccccccattg ccgtgccccc gcccagaata gcaatgggcc
      301 tgtgcccggc tttctgcata tgggccatca ccatcagctg aaggaagtgg ccaacgtgaa
      361 ggctgtcggc tgtgggatca aatccgatat agaaagtgag cttctcttta tcaagcatct
      421 cccggatttt ttcttcattg gtggtctgcg caatcattcc gcgctgtttg agttcttcaa
      481 aaacgttcat gttgactttc atgcctttca ctctaaaatt cagttggata tcccggaaag
      541 ccgaagcatt tcctctgcgt tgcgaagcgt aagctcagtg atatccacac cgcccataat
      601 ccgggcgagt tccttttttc tgccgtcaaa atccagcgcc cgcaggctgg tataagtatt
      661 cccgccccgg atgtgcttct cgatcagaat atggcgatcg gcctgggcgg cgatctgagc
      721 aagatgggtt acacagacga cctgtctggt tttggaaacc tgccggagcg acatgccgat
      781 tttttgagcc gctttgccgc ttacgcctgt gtcaacttca tcaaaaatca gtgtgtcaat
      841 atcatcactg cccgcaagca ctttcttaat cgcaagcatc acccgagata tttcgccgcc
      901 ggatgcgatt ttcgcaagcg gccttggcgg ggaacccgga tttgcggata tcaaaaattc
      961 cacccggtcg gtcccgttgg cggccggctc ctgcggttca atctgaataa caaatttaac
     1021 gccggggcat atcgagaaat tccagttcgc tttttacgcc gcgctccatt gatttacacg
     1081 catgatgcct tgcgcggctc aaagcgcccg cattctcggt aagtttattt ttctcctgat
     1141 cgagcatctt ctttagcttt tgcgcttttt catcggatga ttccattccg tcaagttctt
     1201 tttttgcatt ttcaagataa ctcatgattt cgggaactga gccgccatat tttaatttaa
     1261 gacgatagat catgtccagg cgctgctcga tccagtcgag atcttcctga ttatattccg
     1321 tttcattcat gccggatctc agctcgctca cgcaatcttc aatttcatag gaaagatcgg
     1381 tgatacgctg agcgagactt ttataatccg tattatattc cgcggcagcg gcaagacatt
     1441 ctcccgccgt ggatacaagc tctcgggcgc ctctttccgc ggaatcggtg ttttctgccg
     1501 tttcattgtc accgctcagc agcgtataag cccgattgac cgactgcgtg attttttcgg
     1561 cgttcacaat cacggttttc tgcgccgcga gatcttcctc ttcgccgggc tttagttccg
     1621 ccgactcaat ttcattaatc tgatagctca gcagctcaat ccgccgcacg cgggccgttt
     1681 catccgtgag tatcccggaa agttcccgtt caatttgttt caccctgcgg taagcttctt
     1741 cataccggct gagaagctcg tgcgtctgtg aaaaacgatc aagatagaaa atgtgtttgt
     1801 cgggagaaag aagcgcctga ttatcatgct ggccatggat gttgacaagt tttcggccaa
     1861 tctccctgag catggaaacc gttgccggac ggcccgatat ccggcaggaa cctctgccgt
     1921 cggcggaaat ctcccgctga atcagcaagc tgccgtcttc ctcacactcc atccccaatt
     1981 ccctgaccaa tttctccgct tcctccgaga tttcggtaaa aagggcgccg acgagggctt
     2041 ttctgctgcc ggtcctgaca atgtccttgg taacccgttc ccccaaggca aggttgatcg
     2101 agtcaatcag gatagacttc cccgctccgg tttccccggt cagaacattt aatccacgtt
     2161 taagatttag atccgcgttc tcaatgaccg ctacgttttc aatatggagc tgcgacagca
     2221 ttttatcacc cgccctttct ctttcgcttg aatcaggaca atttttcgat aaaaaagtcg
     2281 tataattctt ttgcgtcctg ctcatttcgc ataataacaa aaattgtatt gtccccggct
     2341 aaggaaccca cgattttctc aaaatgcatc acgtccataa tttcgcataa cgcattgccg
     2401 tggcccgcgt agcacttgat gattacctgg ttcatcacat aatctacttt aataacagat
     2461 tcgttcaaga gaaaatcaaa ttttgcggat aaatcattag acggcatttt tcctgtcgaa
     2521 tagatatatt ctccattttt ggacagggtt ttgaccagcc ggagctcttt aatatctctg
     2581 gaaacagttg cctgggttac gtcaaaaccg tttttgcgaa gcatttcgag cagcatctcc
     2641 tgagtcccga tatcatgctc cttaatcagc tcgatgattt tcttatgacg gctctgtttc
     2701 atacaattag ataccccttt cggataattt ctggttaacc acctcataaa aggtattatc
     2761 tttaagcctg atcagttttg ccttgtggtc tgcgcgtgta attgacacct gatcatcgtc
     2821 gctgagctga atggtctcct gcccgtcaac cgtcaggaac acttcttttt cgtttgcttt
     2881 tccggttacg atcccgattt tggacgccgg agaaaaaagc agtgacctgg agatcagcga
     2941 atgggggcag atcggaatta ccccgatgct ttccagaaca ggatcaatca ccgggcctcc
     3001 ggcagataac gcataggccg ttgaaccggt gggagtcgat acgatcacgc cgtcagccct
     3061 gtaaccgctg acccgcctgc cgttgcaggt aacttctata tcgatgattc tcgacagtgc
     3121 tcctttggac acgacggcgt cattgagcgc atggaatact tttttaccgc gggctcctgt
     3181 gatctcgatc ctgagcagca tccggtggtc gatctgatag tccctgccga tcagctttga
     3241 aagagattcc agctcatacg cctcaatacc ggccatatat cccagtctgc cgacgttgac
     3301 tccaagaaca ggaatattcg tttgggcagc ttttttcgca atatgaagga tggtgccgtc
     3361 ccctccgagg gcaacgataa tcccggcttg ctgataaatc tcttcttttt ccagtttccg
     3421 gaccggggaa tcctcaaaac ccggaacttc ccgtgaaacc aaaacctgag ctccaagcga
     3481 gacaagacgg gaacatatcg cttttgtgca gcaataattc agatcccgtt cctcattcgc
     3541 aatcaccgca attttcaatg cgcttcacca ccgtcaagct ctttgtgaga gagtattacg
     3601 acatcttcta ttttaaacaa attttcatgg gtttgacaag tcttttttaa atatagtaaa
     3661 tattcaatgt ttccttcggg gcctttgacc ggagaaaagg taataccttc aacaacaaag
     3721 cccaattttg cagcgaaatt gcagaccttt tcaattacct caacatgtac gcgcggatct
     3781 ctgaccacgc ccttctttcc gaccttttcc cttccggctt caaactgggg cttaatcagg
     3841 caaacggcct cgccattgtt ttttaaaaaa cgataggcaa ccggcagaac aagagtcagt
     3901 gatataaacg aaacatccac cgagaaaaaa tcaacctgtt cgccaacctg ctcgtcggtt
     3961 agataacgaa tatttgtgcg ctcaaagttg acgactttcg gatcatttct gagtttccac
     4021 gcgagctgcc cgtaacccac gtccacggaa taaacttttg aagccccgtt ttggagcata
     4081 cagtcggtaa atccgccggt ggaggcaccg atatccatac agaccttacc gttcatttcc
     4141 agtccaaaag catccatggc tttttccagt tttaatccgc ctctgctgac ataccgaagc
     4201 gtatttcctc tgatttcaat ctgggaatcc ggattgattt catcgcccgg cttgtccact
     4261 ctttgattgt cggcaaaaac gaggccggac ataatcaaag cctttgccct ctcgcggctc
     4321 tgctccagac ccttctcgac cagcaaaacg tcaattcttc tttttcggct cattttcaca
     4381 ctcccgtaat actgcttcaa ccattccctg cgtgtccagc ttcaaaattg aaagcagctt
     4441 atcgacgttc ccctgcgcta caaactgatc gtcgattgca taaatttttg atgaaccctg
     4501 aaatcctttt ttattcaggc cgtccagaaa attttccccg acaccgcctg aacggattcc
     4561 ttcctcgaaa aaatataccg atttaaaata agaagtgatc tcaaaacaat cttggtcgat
     4621 tggcttgatc cggttgagct ttagcaccga aacattccgc ccggttctct tcaattcgtc
     4681 cgccgcagcg gacgcgtatg caaaaagcct gccgtatgtg atcagcagaa tatccgcgct
     4741 tccgcttcca taaagatcaa agctgccgaa agacgggtgg aaatcggctg gaagcatggg
     4801 ctcgcagccc ctcggatatc gaacacacgc agggccttcc gtttcataac aggcggcata
     4861 cagaaaattc tgcagctcat cgttggtcgt agggctgtaa acaaccattc ccggaatggt
     4921 atttaaaaac gctgcgtcaa aaaccccctg atgcgtctcg ccatcctcgc cgacaagtcc
     4981 ggcgcggtca atcgcgaaaa cgacttttag gtcttgaagc gcggcgtcat gaagaatctg
     5041 atcatatgcc ctctgcagga aggtagagta aacggcgaac acaggaatca taccgtttct
     5101 ggcaagtcct gcggcaaaaa caacagcatg ttcctcggcg atacccacat cgaaaaaacg
     5161 ctgccggtaa gtataggcaa agtccgtaag gccagttccg gcggccattg cggcggtaat
     5221 cgcgcagata gactcatcct tttcagccag tctgatcagc gttttcccaa atacatcgga
     5281 aaaacttgcc cgcccgctgc cagctacaag atcccccgtt tcaacatcaa actgggatac
     5341 accgtgaaag gttctgggat ccgcttccgc aaatgtatag cctttcccct ttacagtcat
     5401 cagatgaatc aggcagggcc ggtttatcgt tttcgcccgt tcaagaaggc ggcatatctc
     5461 ctctatgtta tgcccgtcgc cggggccaag gtagacaaaa cccatatctt caaagattgt
     5521 gctgtgataa agcgtatttt taatggcgga ttttgaagcc accatgattt ttctcagttt
     5581 tttcccgata atgccggtgt gatttaaaat atactcgata aaatccttgg ttttcagata
     5641 acccggtttc gcccgaataa cggcaagatg acgcgcgatg gctcccacgt ttttggatat
     5701 ggacatttta ttatcattga gaataacgat aagattgtca tggctccggc cggcattatt
     5761 cagcccttca taggcaagcc cgccggtcaa tgcgccgtcg ccgattaccg cgatgacata
     5821 gccgtcttct tttttcaggg ttttggcctt tgccataccg taagcagccg atattgatgt
     5881 gctgctgtgc cccgctccga acgggtcgtg agcgctttca taggttttgg tgtaaccgga
     5941 aagcccccca tactggcgaa ttgtttcaaa ccggtttctt cggccggtaa gaagtttgtg
     6001 ggtgtaacac tgatgaccga catcccagac aatctggtct tccggtgaat taaaaactct
     6061 gtgaagcgca accgtaagtt caactacgcc aagattggat gagagatggc cgcccgtgtt
     6121 ggagacggtg tcaatcagtt cgttacgaat ttcctcacac attatattaa gttgcttttt
     6181 gcttaaatat tttaagtcct caggactgtt aattctatca agcagacgat tcttatccat
     6241 tcagagcctt cttccaaata tgctgcggaa taaaaaatca tttaggccat cgccagtgag
     6301 acaagtattc cgagaagggc ccccgctaaa acttcgaccg gtttatggcc caaatgttct
     6361 ttgagatctt tgtcggtaat ttccgtcccg tcctcttccc acttgtcgac cagcttattc
     6421 agtattttgg cttgctctcc cgcggcgcgc cgtactccca tcgcatcata catgaccacg
     6481 gcggcaaagg ccaccgcaag ggcaaattcc ggcgaagacg ggccgcattt tcgtgcgatt
     6541 cccattaaaa gagcgcagac cattgcggaa tgtgcgctcg gcattccgcc ggcgccgaaa
     6601 attctttccg tgtcaaacct ttttgtactg ataagcgtca ggatggtttt tatcacctgt
     6661 gacagcatcc agcttacgac tgcggaaacg aggatgtaat tctttaaaat cagatataaa
     6721 accctcatcg gagtcaattc ccttctttta cctgtcccga tttgcaagag cctctgcaag
     6781 aagcgacaaa aattcgctct ttggcccaaa tccttttaaa cttagaacgg cttcatccgt
     6841 caatttttga accagaactt ttgatttttc aatccccaaa tgagtaatat aggtcgattt
     6901 atcgttatct tcgtcactgc caacgggctt accaagagtt tcgttgtcac tggtcgcgtc
     6961 aagaatatca tccacgattt gaaaagcaag cccaagatta tcggcatatc ctgcggccca
     7021 ttgaatttcg ttctctccgg cgtccgccag aatacagccc attctggcac ttgcggtaat
     7081 catagcgccg gtcttcttat tatgcataaa tttcagcgtt tccaggctga cctgatgacc
     7141 ttcggactca agatcaataa tctgaccgcc gaccataccc tctgctccgg aggctcttgc
     7201 cagctcataa acggctctca gtgcattttc cggtttgatt ttacccttgt aattattgca
     7261 taaagccgtt tcaaatgcaa gacttaacag cgcgtcgccc gccagaaggg ccgttgcctc
     7321 gccgaaccgg atatggcagg aaggcttgcc gcgtcttaaa tcatcgttgt ccatacaggg
     7381 aagatcgtca tgaattaaag aataagtgtg gatcatctca actgcgcagg cgaacggaag
     7441 cgtagcctcg gcatctccgc cggctacttt gcagaattca agcaaaagcg tcggccggat
     7501 gcgttttcca cctgccatcg cgctgtaccg catcgcttca caaacgtctt tttgcgggca
     7561 ggatgtatcc ggcaggtatc tgtccaaagc tttgttgacc atatccgtat attcatttat
     7621 acgctgttca accaggttat tgttcatcgt tggcttctcc gttttctttt tcactgtcgt
     7681 ttattttaat gatcttctgt tcggcttcgg tgagcttccg gctgcagagg accgaaagat
     7741 gcgctccctc ttcaaaaagc ttcattgatt catccagtgt gacgtcgccc tgctccagta
     7801 attccacaat tttttcaagt cttgaaattg cgctttcaaa tgtcatttct tttttcatca
     7861 tttccgcctt tcgcaacgcg tgattttatt tcgctgccac aggttttttt cttttcttac
     7921 cgagggcttc aattccggac actttgcatt ttgcggtacc gtccgaaagc cgtagatcaa
     7981 tttcatcatc cggtaaaaga cccttaacgg atgaaagaat atccccgcgc cggtcataag
     8041 caatggaata gcccctcgag agtaccttaa gcggacttaa cgcatccagc tttgcggcaa
     8101 gttcccgaaa acaggcattt tgcaaagaga cttcagcacg ggcagcgccc gccaggtctg
     8161 cggtaagcct gtccaacttc atccttcgga attccacgtt atatagcggc tctttcagcg
     8221 ctcttgtgga agtgacgtct cttaaccgta cctttcgatc atttatttta ttctttgttg
     8281 ctttttcaat tgaaaactca agcgatcgga cgtattcggc tactgcggca agatccggcg
     8341 aaaccagctc tgcggcggcc gaaggggtcg gagctcgaag gtcggcggca aaatccgcga
     8401 ttgtgtagtc ggtttcatgt cctactgcgg atattaccgg aatccgcgaa tctacaattg
     8461 tacgcgccac cgtttcgtcg ttaaacgccc aaagctcctc aatggagcct cctccccggc
     8521 cggtaatcag cacatcaacc ggctgccgct cgttaaaata acggattgcc ctgcagatct
     8581 gacggggcgc ttcttcgccc tgaacaagaa ccgggcagag caatatctca gccaccggcc
     8641 agcgccggtt taatatttga agcatatcct gcagtgcggc gccggtggga gaagtaataa
     8701 tgccgatgcg ctcaggatat tccgggatcg gttttttatt tttttcatca aacaggcctt
     8761 ccctgcccag cctttccttg agctgctcat aggcggtgtg aagcgctccg accccgtccg
     8821 gctgaagatc gttaatataa aattgaaact gcccgtcccg gtcgtaaagc gacgcccttc
     8881 ccatagcgat cactttcatt ccgttctgcg gcagaaattg aagacgctgc gcactgcttt
     8941 taaacattac cgccttgatg acggcgcttt catctttgag cgtcatataa agatgcccgg
     9001 aacgatagtg attcgtaaaa ttagaaattt cccccctgac aaataaatta atcaggttgg
     9061 gatctgattc cagcagggat ttcacatacc ggttcagctg ggaaaccgac agaattataa
     9121 aaccattatt catgagtaat cagccctcag tttggtcagc accgcaatgc cggccgcgtt
     9181 gtcggaagaa aaagcgggct ccgcaaatac cgcgccgtat ttcccggtca ttttttcgcg
     9241 aatggaccgg ttggacataa ctccccctgc gtaaagcagc ctggagccgg gatgatcccg
     9301 cagcaggccc tgtgtcatgg cttcgatgac cgcctcgacg gagtccatgc agaattttgc
     9361 aatgtcctca tccggctctc cgcgtctttt catatcggcg cattgatttt caatcccgga
     9421 aaggcagcag tccggccctt tcatgcaggg tttaatttta aactcccggc tgctctgcaa
     9481 tgcaagtttc tccagcgcgg ggcctgcggg aaagtcaagg ccgagcagca gcccggttct
     9541 gtcgatcagc tggcccgctt ttaaatcaag cgattttgct ataaggctta tgttaaattc
     9601 gagaggattt cccgggcaat agagaacggc ctctgtggtc cctcccgaaa catgaaacgc
     9661 aataaatttt tctttcagca tctcaagtct gccggctgaa aaaagagcgg cgacaatatg
     9721 ccccgcctga tgtgaaaagt cataaaacgc aatgcccgcc gccgaagcga cggagcgtgc
     9781 aacacattca cccacggtga agcagggcat gtaggagcct tcggcatctc ttggtcttat
     9841 ggaagcgccg acggctttga ccgcgaaccc gctgccatcc atcaattttc cgatcatttc
     9901 gggcagctgc cggacatgct gaaacaccgc gtcgctctgt ttgagccctt tttcgccctt
     9961 tttcacccaa aggagcttct tttgttgata aatcgcacct gtttccccgt cataaacggc
    10021 tgctgaggtg gtatagttgc tggtatcgat tccaagataa aaccccatcg gactattccg
    10081 cctcttcgct tgtttcgctt ttctcagttt tctcggattt ctcaatattt ttggcctttt
    10141 ccggatcttc ccctttttcg ctgcggacaa acgcccccag cattccgtta ataaacgcgc
    10201 cgtcgccatt tccggcatac ttttttgaca gctcaaccgc ctcgttgatg ctgacgctta
    10261 tgggaatttc cgatttgtat gaaatttcaa agattgcaag cctcaagatg gacaacacaa
    10321 ctttggaaag tcttgatttg ctccacccaa ttgaatattt ttcaattgtt ccgtcaattt
    10381 cgtccagatg ctcaatgacc ccggccgcaa gctctttcgc aaacgggtcg ggggttaact
    10441 ctcgggactc tatagccagc tcgataattt ccggaagcgg ctcgttgtta aacaaccttt
    10501 caaatacaag acagagcgcc tgttcccttg cttctcttct attcatcatt tccctctcgc
    10561 tttaacttca atttttgatc acagcccgca ggacaaaaga tggattcgtt ctaacgcggc
    10621 tttccatttc aaaaactcat tattaagatt atgtgccaac acgcttttat gtatgccgga
    10681 aaatattttg ccgcggaagg aacaaccggg tccgcgccgg ataatgccct gcctgccagc
    10741 cgccatacgg cacgttaaaa gaaaccctcc agaataactg gagggttatc cgaggctatt
    10801 gtttgccgcc ggcgccctgg tcaatgacaa ctcccgaaac atgaacattg acttttgaaa
    10861 ccgtaatgcc cgtcatattt tgaatagatt ccttcacttt gtcctgaatt tcagaagcca
    10921 catccggaat tttaaacccg ataaacagat tgacataaat atcaataacc gcttccccgt
    10981 ctttcaattc caaccggatg gtttttccaa ggcttctgtt tttcataaag cccctcggct
    11041 cagggcccgg gcgcaatgac aggcttgcaa cccctttgat ttcccttgct gtcatccctg
    11101 ctacggttgc cagtacatct tcagaaattt taagggttcc cgcatctgcg taatgatttt
    11161 gttccatatc acgcgccctc ctttccctct attataccac atgttgc
//
LOCUS       DATALY010000389         5931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_859581,
            whole genome shotgun sequence.
ACCESSION   DATALY010000389 DATALY010000000
VERSION     DATALY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859581"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VHR42_10665"
     CDS             complement(<1..882)
                     /locus_tag="VHR42_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3027666.1"
                     /translation="MKQDTFRQIRNSLEKVFRSSPFYKNIFAAAGAEFEDIQTPEDFE
                     KLPFTDKNALREAYPLGLLAVPDREVVRIHSSSGTTGSPVIIPYTKQDVEDWATMFAR
                     CYEIAGVTDRDIIQITPGYGLWTAGIGFQAGCEKLGAMAVPMGPGNTDKQFRMMMDLK
                     STVLCSTSSYALLIAEEAEKRGILNSLHLKKGIIGSERWGELMRGRIREGLQIDLFDI
                     YGLTEVYGPGIAIDCEHHAGLHYFDDYLYFEIIDSETGEVKPAGEWGELVITTLKKQA
                     APLVRYRTHDITRLIDGT"
     gene            complement(905..1501)
                     /locus_tag="VHR42_10670"
     CDS             complement(905..1501)
                     /locus_tag="VHR42_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX3027667.1"
                     /translation="MKFDILIAGVGGQGQVLASRILGAAAIADGKSARTGETIGMAQR
                     GGCVVSNVRIGDVSAPTVPIGEADLLIGFELCETARNLSRLAPGGKVILNRQMINPIT
                     VSLGFAEYDAEAMLARIRQKAGGLLEVDAVSLAGQAGSARAANVVLLGAAAGMGNLPF
                     PKETVLRAVEENVAAKFHDLNVRAFELGFDYAQSKQKK"
     gene            complement(1498..3291)
                     /gene="iorA"
                     /locus_tag="VHR42_10675"
     CDS             complement(1498..3291)
                     /gene="iorA"
                     /locus_tag="VHR42_10675"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX3027668.1"
                     /translation="MKKLLMGNEAIAFGAMKAGVRVVCGYPGTPSSEVLETVAKRNDG
                     SIYVEWSVNEKAAMEIGAGASYTGARVMVTMKQVGLNVASDPLMSLSYIGVKGGMVIL
                     VADDPGPNSSQTEQDTRHFAKFANLPVLDPSSPEEAYEMACYAFELSEKTGLPVFLRP
                     TTRICHACGTVEVSDEKEVHKAEGFVKDPKWVIFPALSYQNHGRLEKLQTEIAETFDR
                     SRFNRIQGEGRLGIAASGVAYAYCADVLKKHGFGGKLFKMGTPYPFPENLAGRFLMGL
                     ESVMVFEELDPVVEDALIRTAALKGLKVKIRGKATGDVPNRGELSYEIVERAILNYCG
                     VQQQDHTTPTAALSLPARPPVLCAGCPHRASFYAVKQAMKGKKAIFAGDIGCYTLGNA
                     KPLEMVDTCLCMGASVTVAQGIKRVEPNASLFSFIGDSTFFHTGIPGVINALYNGTKI
                     TLIVLDNSTTAMTGHQPHPGIGKTMMGHDSVKVSIYNTLKGIGVPYVKRVNPFDLKRA
                     VETVREAESQEGVSAVIFEAPCIAIYKKTEVLTVAQEKCKKCGKCIREIGCPAMYLRK
                     DRSAAIDPTLCYGCGLCAKICPFDAIGREDQ"
     gene            complement(3868..4842)
                     /gene="pfkA"
                     /locus_tag="VHR42_10680"
     CDS             complement(3868..4842)
                     /gene="pfkA"
                     /locus_tag="VHR42_10680"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX3027669.1"
                     /translation="MGKEMKTIGVLTSGGDAPGMNAAIRSIVRAGIANNLKVIGIRYG
                     YNGLINGDLVEMNLRSVSDIVHRGGTILYTARSPEFKTEEGMNKAVKVCHDAKIDGIA
                     VIGGDGSFRGATDLSQRGIPCVGLPGTIDNDIASSEYSIGFDTAINTVKDMVDRLRDT
                     TESHDRCSVVQVMGRNAGFIALHSGIAVGATFIIVPELSYDIENVMIPRMLEIEKTGK
                     KHFIIIVAEGVGDVDKIAKTIQDHTGIESRATVLGHVQRGGSPTVRDRVIASEMGYHA
                     VELLSQGIGNRVVVMKDNKVIDYDIYEAFKMTKPFEDKLFHIAQTINI"
     gene            complement(4916..5107)
                     /locus_tag="VHR42_10685"
     CDS             complement(4916..5107)
                     /locus_tag="VHR42_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027670.1"
                     /translation="MWTVIYMAQTKESVLKVQELLAAAELAVRVRPVGEGGKDGFFEI
                     LVPETEAQLAHTILIKNGY"
     gene            complement(5299..>5931)
                     /locus_tag="VHR42_10690"
     CDS             complement(5299..>5931)
                     /locus_tag="VHR42_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX3027671.1"
                     /translation="VIGFYLTGHPLAAYRDYIRQKRLVSVRDILSSSGANRYGDGKTA
                     EILGMIQAKQLKSTRSGKTMAFLTLDDGSGSIEIVVFPPALERFRSVLNVDDVVQIKG
                     KISVREDEAPKLLCDEALTPDLRFSEQKGGKKTVREGLYLRVPSKDGPEYRKAENLVS
                     IFDGDFPLYILFRDRQKLALAPKRMWVSLNNVLLSELKAVLGEENVRVRR"
BASE COUNT         1373 a         1688 c         1376 g         1494 t
ORIGIN      
        1 ggtgccgtcg atcaggcgag tgatgtcgtg tgtgcggtag cgaaccagcg gagccgcctg
       61 ctttttcagc gtggtgatta ccagctcgcc ccattcaccg gccggtttga cttctcccgt
      121 ttcactgtcg attatttcaa aatacaaata atcatcaaag taatgcagac cggcgtgatg
      181 ctcgcagtcg atggcgatcc ccgggccgta aacctcggtc aggccgtaaa tatcaaacag
      241 atcaatctga agcccctccc gaattctgcc gcgcatcaat tcgccccagc gttccgaacc
      301 aatgatgcct ttctttagat gaagggaatt cagaattcct cgtttttcag cttcctctgc
      361 gatcagcagt gcataggatg aagtcgagca aagaacggtg ctttttaaat ccatcatcat
      421 ccggaactgc ttgtcggtgt tgccgggccc catcggaaca gccatggccc ccagtttctc
      481 acagcctgcc tgaaagccga tgcccgcggt ccacagcccg taaccgggtg tgatctggat
      541 gatatcccgg tcggttacgc cggcaatttc atagcagcgg gcaaacatgg tcgcccagtc
      601 ttccacatcc tgctttgtgt aaggtatgat cacaggactt ccggtggttc cggatgagga
      661 atgaatgcgg acgacttcgc ggtctggaac ggccagaagg ccgagcggat acgcttctct
      721 cagagcattt ttgtcggtaa agggcagttt ttcaaaatcc tccggcgttt gaatatcttc
      781 aaattccgca cccgcggcgg caaatatgtt tttataaaaa gggctcgacc ggaacacctt
      841 ttccaaagaa ttcctgatct gccgaaacgt gtcctgtttc atgccagcca tctctccctg
      901 caatttattt cttctgtttg gattgagcgt aatcgaaacc taattcaaaa gcgcggacat
      961 ttaaatcatg aaattttgcc gcaacatttt cctcaacggc acgaagaacc gtttctttgg
     1021 gaaacggcag attgcccatc cccgccgccg ctccaagcag aacgacattt gccgcccggg
     1081 ccgatccggc ctgcccggca agagagaccg cgtcgacctc cagcagcccg cccgcctttt
     1141 gccgaatccg ggcgagcatt gcctcggcgt catattcggc aaagccgaga gataccgtga
     1201 tcggattaat catctgacga ttgagaatca ccttgccgcc cggcgcaagt ctggaaagat
     1261 ttctcgccgt ttcgcacagc tcgaacccaa tgagcaggtc ggcctccccg atcgggacgg
     1321 tcggggccga cacatcgccg atccgcacgt tgctgacaac acatccgcct ctttgcgcca
     1381 tgccgatggt ctcccctgtg cgggccgatt ttccgtcggc aatcgccgcg gcgccgagaa
     1441 tccgggaagc cagaacctgg ccctgtccgc cgactccggc gattaatata tcgaatttca
     1501 ttgatcttcc ctcccaatcg cgtcaaaggg acaaattttc gcacaaagtc cgcagccgta
     1561 acaaagcgtc gggtcgatgg ccgcggaacg gtctttccgg agatacatgg ccgggcagcc
     1621 gatttcacgg atacatttgc cgcatttttt acatttttcc tgagcgaccg ttaaaacttc
     1681 cgttttctta tagatcgcaa tgcagggcgc ctcgaaaatc acggcggaaa ctccctcctg
     1741 cgactctgct tcacggacgg tttcaaccgc acgtttcaaa tcgaaagggt tcacccgttt
     1801 gacatagggc actccgatcc ctttgagcgt attataaata ctcaccttca ccgaatcatg
     1861 tcccatcatg gttttgccga tgccgggatg gggctggtgg ccggtcatgg ccgttgtgct
     1921 gttgtcaagc acgataaggg tgatttttgt cccgttataa agcgcgttga tgactccggg
     1981 aattccggtg tgaaaaaagg tagagtcacc gataaacgag aaaaggctgg cgttcggttc
     2041 cacccgtttg attccctgcg caacagtgac ggacgcgccc atgcacaggc aggtatccac
     2101 catctccaga ggcttggcgt tgccgagggt ataacagccg atatcacccg caaaaattgc
     2161 ttttttgcct ttcatcgcct gtttcacggc gtaaaacgag gcacggtggg gacacccggc
     2221 gcaaagcact ggaggccggg ccggaagtga cagcgcggca gtcggagtcg tgtgatcttg
     2281 ctgctgaaca ccgcagtagt tgagaatggc cctttccaca atttcataac tcagttcgcc
     2341 ccggtttggc acatctccgg tcgcttttcc tctgattttc acctttaggc ctttcagtgc
     2401 ggccgtccgg atcaaagcat cctccaccac gggatcaagc tcctcaaaga ccatgacgct
     2461 ctcaagaccc atgagaaacc gaccggcaag attctcgggg aacggataag gcgttcccat
     2521 cttaaacagt ttccctccga aaccgtgctt tttcagaaca tccgcgcagt aggcgtaagc
     2581 cacacccgac gcggcgatgc caagccggcc ttccccctgg attcggttaa accggctccg
     2641 gtcaaaagtt tccgctattt cggtctgaag tttttcaagc ctgccgtggt tttgataaga
     2701 aagagcggga aaaatcaccc acttcgggtc ctttacgaaa ccttcggctt tatgtacctc
     2761 tttttcatcc gaaacttcta cggttccaca ggcgtgacag attcttgtcg taggccttaa
     2821 aaacaccggc aaaccggtct tttccgacag ttcaaaggca taacatgcca tttcataagc
     2881 ttcctcaggg cttgacgggt ccagaaccgg aaggttcgca aactttgcaa aatgccttgt
     2941 atcctgctcg gtctgggagg aattcggccc gggatcgtcg gcaaccagga tcaccattcc
     3001 gcctttgacg ccgatataac tcaggctcat cagcgggtcg gaggcgacat tcaggccaac
     3061 ctgtttcatt gtcaccatga cccgcgctcc ggtgtaagac gcgccggcgc cgatttccat
     3121 ggcggctttt tcattgaccg accattcaac atagatactt ccgtcgttgc gtttcgcgac
     3181 tgtttccagc acctcgctgg atggagttcc gggataaccg caaacaaccc gaaccccggc
     3241 tttcatcgct ccgaaggcga ttgcctcgtt gcccatgagc agcttcttca tatgcttcac
     3301 cacttaattt catcaatatt ttacacgcgg gcaaataaaa aacgtctgtc cttgtaaaat
     3361 tttacaagga cagacgtatc ttcccgtctg cggtaccacc ttgcttgagc cgcaaatatt
     3421 gcagccccgc ctcgttggaa tatcgaaata ttcccgcctg ttaacgcctg cgacgcgtcg
     3481 gattttactt gccattcccc gaattcccgg gataaaaacg gctttctttc cgccctcggg
     3541 agcccataga gccaactgac tcatgccgcg atcacagcaa cccgcggctc tctgaatgcc
     3601 cagaactggc tttctctccg tcttcggttt ctcatcttat tctctattta aaaatagcac
     3661 aacaccatga cattgtcaaa tcggcgggcc ctgaagccgg atggcatctt ttgccgctgt
     3721 gtctatttca tattatggct ttcagttttt tacgacacac atcccggctg ccgcggatca
     3781 attttaaatt caggcaaaag agaacccttc agccgatttg cggctgaagg gttctctttg
     3841 agaaacggat gtattggaac aagtccgtta gatattgatt gtctgggcaa tgtgaaaaag
     3901 cttgtcctca aacggcttag tcatcttgaa tgcttcatag atatcataat cgattacttt
     3961 gttgtctttc atgacaacaa cacggtttcc gataccctga gaaagaagct ctacggcatg
     4021 atagcccatt tcagaagcaa tgacgcggtc gcggaccgtc ggcgagccgc cgcgctgtac
     4081 atgaccgaga acagtcgccc tggactcaat gccagtatga tcctgaatgg tcttcgcaat
     4141 tttatccaca tctccgacgc cttcggcgac gatgataata aagtgctttt tgccggtttt
     4201 ctcaatttcg agcatccggg gaatcataac attctctatg tcataagaca gctcaggaac
     4261 gatgataaat gtagctccga ccgcaatacc ggaatgtaac gcgataaaac cggcgtttct
     4321 tcccataacc tgcacaacgc tgcagcggtc atgagactcg gtggtgtcac gcagacggtc
     4381 gaccatatcc ttaaccgtat tgatcgccgt atcaaaacca atggaatatt cacttgaagc
     4441 gatatcgtta tcaattgttc cgggcagccc gacgcaggga attccgcgct gggaaagatc
     4501 ggttgctcca cggaaagaac cgtcgccgcc gattactgca ataccgtcaa tcttggcgtc
     4561 gtggcaaact ttgaccgctt tattcatacc ctcttcggtt ttaaactcgg ggctgcgggc
     4621 tgtatagaga attgtgccgc ctctgtggac aatatcggaa acactgcgaa gattcatctc
     4681 gacaagatcc ccgttaatca gaccgttata tccatagcga atgccgatca ctttcagatt
     4741 attggcgata cccgctctta cgatggagcg aatcgccgca ttcataccgg gagcgtcgcc
     4801 tccgcttgtt aaaactccaa tcgttttcat ttctttgccc atcttctata tcctcccaaa
     4861 acttgacttt tgtcgtcaac tcacaaagaa cattatacaa ggttaaaatg atgtgtcaat
     4921 acccattttt gattaggatt gtatgcgcaa gctgcgcctc ggtctcgggc acaaggattt
     4981 caaaaaatcc atcctttccg ccttctccga cgggacggac cctcaccgca agctccgcag
     5041 cagccaataa ttcctgaacc tttaataccg actccttcgt ctgagccatg tatatcacag
     5101 tccacatgct tacccctccg cctgtaccgt tgccgtcaag cttgtacggc atccggttga
     5161 acgatcaatc taatctttct gcattgtatt ccgtgcggaa agatgattat accttttaaa
     5221 ttttcttttt cgattttctt ttttcgttga gtcgacaatt attatattat accatttttt
     5281 ttaaagttca accactaatt atcgccgaac ccgaacattt tcttccccca gcaccgcttt
     5341 aagctccgaa agcagcacat tattcaggct cacccacatc cgtttcggcg ccagagcaag
     5401 cttttgccgg tcccggaaca gaatatacag cggaaaatct ccgtcgaaaa ttgacacgag
     5461 gttctccgcc tttcggtatt ccggcccgtc tttggacggc acccggagat aaagaccctc
     5521 ccgaaccgtt ttctttccac ccttttgctc tgaaaaccgc aggtcgggtg tcagcgcttc
     5581 atcgcacagg agtttcggcg cctcatcctc cctgacgctg atcttccctt tgatttgcac
     5641 aacgtcgtca acattgagaa ccgagcggaa acgctccaga gccgggggga aaaccacaat
     5701 ttcaatcgaa ccgcttccat catcgagcgt cagaaacgcc atggttttcc ctgagcgggt
     5761 cgatttgagc tgttttgcct gaatcattcc gagaatttcc gctgttttac cgtcgccgta
     5821 acggttcgcc cctgacgagc tgagaatgtc cctgaccgaa accaggcgct tctgccggat
     5881 ataatcccgg taagcggcaa gggggtggcc ggtcagataa aaaccgatga c
//
LOCUS       DATALY010000390         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_772639,
            whole genome shotgun sequence.
ACCESSION   DATALY010000390 DATALY010000000
VERSION     DATALY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772639"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1047
                     /locus_tag="VHR42_10695"
     CDS             253..1047
                     /locus_tag="VHR42_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020619665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX3027672.1"
                     /translation="MARIQCDFFSNSLMMNTSVTVILPTIDREEQSVLTSRFQEGKKF
                     QTLLLLHGMFGDHTDWSRSTQIETYAEENTLAVVMPSAENSFYTDMKIGGKYWTYVSE
                     ELPRACRAMFPLSDKREDNFVAGLSMGGYGAFKLALRKPENFAAAASLSGVMDISGLV
                     NDPDVSKELIAAIYGGVDQLKTEENDLYLLAKINALKKSAAPMLYQACGTEDSLYQDN
                     IAFRDYLESLGLVHAYEEGPGVHDYYFWNAYIKKAIEWMPLKRTAV"
     gene            1093..1470
                     /locus_tag="VHR42_10700"
     CDS             1093..1470
                     /locus_tag="VHR42_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027673.1"
                     /translation="MQRKKALKTMYKDFSPSGEYAKFKEANGEDTFLKMNLLYAAFLL
                     DGLYRQENSDEFDDLCEDVLELSLAEEFDRFSMEDVAEAVEYIIRDSNYTIQEYHKAY
                     KRDGERVTEELIALLDSGKLNED"
     gene            1711..1863
                     /locus_tag="VHR42_10705"
     CDS             1711..1863
                     /locus_tag="VHR42_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027674.1"
                     /translation="MFLVKIKWLGSDYSAMDERRKGYDSENVSKRTRIAALIGGLSMC
                     FLAHLL"
     gene            complement(1979..3379)
                     /locus_tag="VHR42_10710"
     CDS             complement(1979..3379)
                     /locus_tag="VHR42_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3027675.1"
                     /translation="MNITDLISKQNIWWKTGKMDGSRLYKRIRTEFSEIVKNLYNRKI
                     IYLSGPRSVGKTTLLFQTADYLLNIRVNPSRVLYMNGGEFSFALQKPSIREIIENYAA
                     YVVNKNIDQLTDPIYILIDDVHTFKDWQIYLQYYVEKGYNFKFLVSGPTPVPYLPDDL
                     TFFERIAEIPVYPLTQSQFVEFYCLYKEIDFDLVKYKSVLPEYDIFGNPGKFFEAVSS
                     YLPQLKTFQAGKIRMIQEYLLAGGYPGYFGSDSITSWHKRLADEIVEHSIYRDIVCIN
                     SIKSPENLKRLVYLIASMNGTEQSFAALGKMLKIDTVTVMSYLSYLSFNNLIRVSENF
                     SGNTGKIVRKNKRFYTIDCGVKNALSGNTDLLPNDIYQSITNSCLLTVSDYAKKRNIP
                     IGFWRDNKRETDIILQSESRLLPIKIAHDNFIGNREIKDLLYFTNGHKSDSAIFITSD
                     LLKKDGDIYFIPYWLF"
BASE COUNT          947 a          713 c          862 g          925 t
ORIGIN      
        1 ggtgtcgctg gaggccggct tcgaaagtct gagccatttt taccatctgt ttaagctccg
       61 ctacaacgtt acgccggcgg atctgagaaa cgggaaagcc tgatcctcta aatcttgaca
      121 gggtggcctg tttcgcgtaa aatacaaatc ggtatgtgaa ttttcaaccc ggttttcgcc
      181 gggtcagggc agccaaaaag ggcggggccc ttcatttgga cgcgtgccct atatcgacgg
      241 gaggttttac ccatggcgag gatacagtgc gacttttttt caaacagcct gatgatgaat
      301 acttcggtta ccgttattct cccgaccatc gacagagaag aacaaagtgt gctcacatcc
      361 cgttttcagg agggcaaaaa atttcagact ctgcttcttc tgcacggtat gttcggcgat
      421 cacacggact ggtcaagatc cacgcagatt gaaacctacg ccgaagaaaa cacgcttgct
      481 gtcgttatgc catcggcgga aaacagcttt tataccgata tgaagatcgg cggaaaatac
      541 tggacttatg tcagcgagga gcttccgagg gcctgcaggg caatgttccc tctttccgat
      601 aagagggagg ataattttgt ggcgggcctt tcgatgggcg gttacggggc gtttaagctg
      661 gcgctcagaa aacccgaaaa ttttgcggcc gccgccagcc tgtcgggagt gatggatatc
      721 agcgggctgg tgaatgaccc ggatgtttcc aaggagttga tcgccgcaat ctacggcggt
      781 gtcgatcagc tcaaaacaga ggaaaacgac ttgtacctgc tggcaaaaat aaatgctctg
      841 aaaaaatccg ccgcaccgat gctgtatcag gcctgcggaa cggaggattc cctctatcag
      901 gacaatattg cgtttcggga ttatcttgag tcccttggcc ttgtgcatgc ttatgaggag
      961 ggccctggcg tacatgacta ttatttctgg aatgcctata tcaagaaagc catcgagtgg
     1021 atgccgttaa aaagaacggc ggtctgaccg ctggcggccc gggtgggaac ccggcgacag
     1081 gatgatcata aagtgcaaag aaagaaggcg ctgaaaacta tgtataaaga tttttctcca
     1141 tccggagaat atgccaaatt taaagaggcg aacggtgagg atacgttcct gaaaatgaat
     1201 cttctctatg cggctttttt gctggatggg ctgtatcggc aggagaattc ggatgaattt
     1261 gatgatttat gtgaagatgt gctggagctt tcgctggccg aggagtttga ccggttcagt
     1321 atggaagatg tggctgaagc ggtggaatac attattcggg acagtaacta taccatacag
     1381 gaataccata aggcctataa gcgcgacggc gaacgggtga ccgaggagct gattgcgctg
     1441 ctggattccg gaaagctgaa cgaagactga aagtgtaatc cgaaagtttg attccgctgt
     1501 aaaggaattc cacattacag acaaaaagcc gctgtccgat tgcgacagcg gcttttttgt
     1561 ggcaggaaaa cattgacaat cagcagaaaa tgtcatataa tcatatatat tacaaaatca
     1621 ggaacctgat tttcaaaatt taaggtgact accatgaact ttcgtcttat ttttatatgc
     1681 ggaagcattt tcgttcttgg gggttttttt atgtttttgg ttaaaattaa atggctcgga
     1741 tccgattaca gcgcaatgga tgagagaaga aaaggttatg attcggaaaa tgtttcaaag
     1801 cgaactcgaa ttgcggcgct gatcggcggg ctttccatgt gttttttagc gcatctctta
     1861 taaggttcaa aaggcgttca gtaagaccct gcgttaccca aaggtttcgc agggtctttt
     1921 ttatgccgga attattttat gacaggcgga aatgacatat cgcattcaga accaaaggtc
     1981 agaaaagcca gtaaggaata aaataaatat caccgtcttt tttcaataaa tcgctggtga
     2041 taaaaatggc ggaatcggat ttgtgaccgt ttgtaaaata gagaaggtcc ttaatttccc
     2101 ggttgccgat aaaattgtca tgggcgattt taatcggaag caggcggctt tcagattgga
     2161 gaataatatc agtctcccgc ttgttgtcac gccagaatcc aatcgggatg ttacgctttt
     2221 tggcgtaatc ggacaccgtc agcaggcagg aattcgtaat cgactgatag atatcgttcg
     2281 gtaaaagatc cgtattgccc gataaagcgt tttttacgcc gcagtcaatg gtatagaatc
     2341 ttttgttttt ccggacgatt tttccggtat tgccggaaaa attctcgctg acgcggatca
     2401 gattgttaaa cgacagatag gaaaggtagc tcattacggt gacggtatcg attttcagca
     2461 tttttccaag cgccgcgaaa gattgttccg tcccgttcat tgatgcgatc agatagacaa
     2521 gccgtttgag attttccggc gatttgatgc tgtttatgca aacaatgtcg cggtaaatgg
     2581 agtgctcaac aatttcatcg gcaagtcttt tatgccagga tgtgatgctg tcgctcccaa
     2641 aatagccggg gtaccccccc gcaagcaaat attcctgaat catccggatc ttgccggcct
     2701 gaaacgtctt caactgcgga agataggaag acaccgcttc aaagaatttt cccgggttgc
     2761 caaaaatatc gtattccggc aaaacacttt tgtatttgac cagatcaaaa tcaatctctt
     2821 tataaagaca gtagaattcc acaaactgcg attgtgtcag cggatagacg gggatttcag
     2881 caattcgttc aaaaaacgtc aggtcgtccg gcaggtaggg gaccggggtg ggcccactga
     2941 caagaaattt aaaattatac cctttttcga cataatactg aaggtatatc tgccagtctt
     3001 taaaagtgtg gacatcatca atcagaatat aaatggggtc ggtcagctgg tcgatgtttt
     3061 tgttcaccac ataagccgcg taattttcaa taatttcgcg gatgctcggc ttttgcaggg
     3121 caaaggaaaa ttccccgccg ttcatataaa ggacccggga tgggttgacc cgaatattga
     3181 gaagataatc cgccgtctga aaaagaagcg tggttttccc cacgcttctc gggccggaaa
     3241 gataaataat tttacggtta tatagatttt ttacgatttc ggaaaactcg gtgcgaatcc
     3301 gtttatagag acgggagccg tccatttttc cggttttcca ccaaatattc tgtttgctga
     3361 ttaaatcggt aatattcata caatgccacc gttttatgga agccaaaagg ctaaaataat
     3421 gaaaaacgat agattattca tttctct
//
LOCUS       DATALY010000391         4221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_861254,
            whole genome shotgun sequence.
ACCESSION   DATALY010000391 DATALY010000000
VERSION     DATALY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861254"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1207
                     /locus_tag="VHR42_10715"
     CDS             191..1207
                     /locus_tag="VHR42_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX3027676.1"
                     /translation="MNMDDWVKNTIKSPRKKAMPVLSFPSVQMMGIHVADLIKDSNMQ
                     AEAMKRVADRTDAAAAVSMMDLSVEAECFGSTIRFAEDEVPTVVGGIISRKEDAEALK
                     IPKVGAGRTGVYLDAIHKAMRKIEDRPVFAGVIGPFSLAGRLMDVSAAMLYCYDDPEM
                     VHLVLEKVTDFLIDYCMAYKATGANGVIMAEPLAGLLSPALAQEFSSPYIKKIVDHVQ
                     DRDFILIYHNCGGSTVRIIDSILTTGAAAYHFGNAIQMSEMLPHIPPDKIAFGNIDPA
                     GQFRNGTPDSIREATLALLKECGSYPNFVISSGCDIPPLSRWENIDAFFAAVREFYDH
                     AVKK"
     gene            complement(1349..1996)
                     /locus_tag="VHR42_10720"
     CDS             complement(1349..1996)
                     /locus_tag="VHR42_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027677.1"
                     /translation="MATKDSRMAGGFAVCGALYIIIFMVVVNVLTSPSYPWAIYPAFA
                     AVWWPLSVILPRRFGSEFFALAGSTLSSLFLIAVNCVSSPAYPWVLFALPIPVLWPVF
                     QCFGGRAAKLSFALPVSLLLFLYYAALNVTIAKGFPWCMFILYAAAWWPLSIFFAHRG
                     HGFGLSVAGAVITSVLFFALNRITTPNQIWFIYPVFAVLWWPLSVYFFSFRRARA"
     gene            complement(1986..2399)
                     /locus_tag="VHR42_10725"
     CDS             complement(1986..2399)
                     /locus_tag="VHR42_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX3027678.1"
                     /translation="MKQIQEHVEWLFRDVPDGERKSQLMQEIEQNLEEKVKDLRTQGK
                     SEEDALNKAFVDFGDIDEIKAELKTGRRGNDGSLALGFSIWGSVLIIALVVFINFYYT
                     PHVIWFVYPTFGVLWWPLALFFHWLKKRGELRDGD"
     gene            complement(2396..2725)
                     /locus_tag="VHR42_10730"
     CDS             complement(2396..2725)
                     /locus_tag="VHR42_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX3027679.1"
                     /translation="MLSSDIIRGHLDAIVLRLVEEKDRYGYEISKEIESRTTGRFQIK
                     EATLYAVFQRLEKKELLKSYTGSVTFGSKRRYYTITELGRVYLKEETQQWREAKELID
                     IFMEDSK"
     gene            2964..3376
                     /locus_tag="VHR42_10735"
                     /pseudo
     CDS             2964..3376
                     /locus_tag="VHR42_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390690.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
     gene            3398..3946
                     /locus_tag="VHR42_10740"
     CDS             3398..3946
                     /locus_tag="VHR42_10740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3027680.1"
                     /translation="MKHLGTKTLETEVAIEEREILANLLEKYLYEFSQYDGCDVNKLG
                     LYGYKYLDCYWLEDKRWAYFIEVDGKLAGFVMVNGVSEADDRETDYSVAEFFVMVKYR
                     RQGIGKRAFFMTADLHRGRWQLKRHPKNKGSVYFWDKAVDEYTKGKYELVKSYPNTEY
                     ADGTPGDIFFFDNSQLDKNEVK"
     gene            3948..>4221
                     /locus_tag="VHR42_10745"
     CDS             3948..>4221
                     /locus_tag="VHR42_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3027681.1"
                     /translation="MIETQRLTVRRFTAGDWKDLYEYLSDPKVVIFEPYEVFSEDECK
                     REAARRSGDDAFWAVCLKQNQKLIGNIYLVKQDFDTWELGYVFNSSY"
BASE COUNT         1189 a          974 c         1082 g          976 t
ORIGIN      
        1 taaaacggga acgattgtat aattgaagca tcctacatat attccggaag caagtttgaa
       61 agataaatct ttcaaaacgg gagttttgtt ctgcggcttt cgccgcgtgc gcacagaaac
      121 aaggcgcgaa tgcggtctgc aacgcaccag ccaaaacttc cggctcggat cgaaagggtg
      181 gccaataaaa atgaacatgg atgattgggt caaaaataca attaagtccc cccgaaaaaa
      241 ggcgatgccg gttttatcgt ttccctccgt ccagatgatg gggattcatg tggcggacct
      301 catcaaagac agcaacatgc aggcggaagc aatgaagcgc gttgcagaca gaacggacgc
      361 cgcggcagcc gtcagcatga tggatttatc cgttgaggcg gaatgctttg ggtcaacaat
      421 ccggtttgca gaggatgagg tgccgacggt cgtcggaggt atcatatccc ggaaagaaga
      481 tgccgaggca ctcaaaattc ccaaggtagg cgctggacga accggggttt accttgacgc
      541 gattcacaaa gctatgagaa agatcgaaga tcgcccggtt tttgcgggag tcatcggccc
      601 tttttccctc gcaggacgtc ttatggatgt gagcgcggcg atgctttatt gttacgatga
      661 tccggaaatg gtgcatcttg ttctggaaaa ggtcacggat ttcctgattg attactgcat
      721 ggcgtataaa gccacgggcg ccaacggcgt tattatggcg gagccgcttg cggggctgct
      781 ctcgccggcg cttgcacagg aattttcatc cccttatatt aagaagattg ttgatcatgt
      841 tcaggatcgg gattttattc tgatctatca taactgcggc ggctcaaccg tccggattat
      901 cgattccatc cttacaacgg gggcagccgc ataccatttc ggcaatgcca ttcagatgag
      961 tgaaatgctc ccgcacatcc cgcccgacaa aattgcgttc ggcaatatcg accctgcggg
     1021 acaatttcgc aacgggacgc cggattcgat ccgggaagcg acgcttgctc tcctgaagga
     1081 atgcggaagt tatccgaatt ttgtgatctc atcgggctgc gatatcccgc cgctttcgag
     1141 atgggagaat atcgacgcct tctttgcggc agtcagggaa ttttatgatc atgcggttaa
     1201 gaaataaaca tagagcccaa agtaaacaga atccccgttt catgaagaga cggggattta
     1261 ctctatttat aaaccaaaaa aaccgggtca aactctgaag cccccgcgag ataccgcact
     1321 tcgcgcctcg aatatgtttc tggctgattc atgccctcgc tctgcggaag gagaagaagt
     1381 agacggaaag cggccaccac agcacggcga aaacagggta gataaaccag atctgattgg
     1441 gcgttgtaat cctgttgagc gcaaagaaca aaacgcttgt gatcacagcg cctgcaaccg
     1501 aaaggccgaa tccatgccct ctgtgcgcaa agaaaatcga gagcggccac caggcggcgg
     1561 catatagaat aaacatgcac cagggaaagc ctttcgcaat ggtcacgttg agcgccgcgt
     1621 aatataaaaa caggaggagc gaaacgggaa gagcaaagga aagcttcgcg gccctgccgc
     1681 caaaacactg aaaaacaggc cagagcaccg ggatgggcag agcaaagaga acccacgggt
     1741 acgcggggct tgatacacag ttcaccgcaa taagaaagag gctgctaagg gtgctccccg
     1801 ccagagcaaa aaattctgag ccgaaacggc gcggaagaat taccgaaagc ggccaccaga
     1861 ctgccgcaaa tgcggggtaa atcgcccagg gataagaggg actcgtcaga acgttcacaa
     1921 caaccataaa aataatgatg tagagcgcgc cgcaaaccgc gaaaccgcca gccatgcggc
     1981 tatccttagt cgccatcgcg cagctcccct ctctttttga gccagtgaaa gaacagggcg
     2041 agcggccacc acagcacccc gaaggtcggg tagacaaacc agataacatg cggggtgtag
     2101 taaaaattga tgaacacgac aagcgcgatg atgagcaccg agccccagat ggaaaacccg
     2161 agcgcgaggc tgccgtcgtt ccctctccgg cctgttttga gctccgcctt gatttcgtca
     2221 atgtcgccga agtcgacaaa cgccttgttg agggcgtcct cttcggattt tccctgcgtc
     2281 cggagatcct tcaccttttc ctccaggttt tgttcgatct cctgcatcag ctgggatttg
     2341 cgttccccgt ccggcacgtc ccggaagagc cactccacgt gctcctgtat ctgcttcatt
     2401 tcgaatcctc cataaaaata tcgatgagtt ccttggcctc ccgccactgc tgagtttctt
     2461 ccttcaaata tactcgtccg agctcggtga tcgtgtaata ccgtctcttt gagccgaatg
     2521 taacgctccc ggtataggac ttcagcagct cttttttttc aagccgctga aaaaccgcgt
     2581 aaagggtcgc ttccttgatc tgaaacctgc ccgttgtgcg gctttcgatt tcctttgaaa
     2641 tctcgtagcc gtaacggtcc ttttcctcaa ccagcctgag gacgatggcg tccaggtgcc
     2701 cgcggatgat atcgctgctt aacatcccga acctcctttg cgtgatagtt caatctgata
     2761 gagctctatg tgatagagca atgataacat ctattgccat tcatgtcaat aggaatcggg
     2821 ataattttat gcggcatctg ccaattggat gttaaaatcg gtcaaaacgt aatgttattg
     2881 aatcgggcat tgcaaaagaa gaactgtaat ggtaaaattt atgaataatc aggccgcgat
     2941 tatatcatta ggggggaaaa ctaatggaat gccgtatgtg tgaaaaccta aaaaatataa
     3001 agaatgaccc ttactttatc atggaactgg aaaccggcta tgtggttctt ggatggtatc
     3061 aaaggtttcg gggttatacg gtattttaat gcaaaataca agcggaggaa ttgcaacagc
     3121 tggaacccgc tttcaaaata aagtttcttg aagaaatgtc ctcgttgccg aagctgcttt
     3181 cgatgtattt aagcccgata aaatgaatta tgaaatgctc ggcaacgggg tttgtcatct
     3241 ccactggcat taaagcccgg tatggtggct gcccaaagat gatttgtttg atgaagcaac
     3301 aagaccaaat gaaaaacaaa gaaacgaaat gattcggaaa atcagaaata aaatcgaacg
     3361 gctaaataaa aagtaaacat gtcggaggtg cggcgcaatg aaacacttgg gcacaaaaac
     3421 gctggagacg gaagtcgcga tagaagaaag agaaatcctt gccaatctgc tggaaaaata
     3481 tctatatgaa ttttcccaat atgatggttg tgacgtcaac aaacttggcc tttacggcta
     3541 caaatatctt gactgctatt ggctggaaga caaacgatgg gcgtatttca tcgaagtcga
     3601 cgggaaactg gccggttttg taatggtaaa tggtgtttcg gaggctgatg acagggaaac
     3661 ggattattct gtcgccgaat ttttcgtgat ggttaaatat cgacgacagg gtattggaaa
     3721 gcgggctttt ttcatgaccg ctgatttgca tcggggaaga tggcaattga aaagacaccc
     3781 gaaaaacaaa ggctccgtat acttctggga taaagcagtg gatgaatata caaaaggtaa
     3841 atatgagctg gtcaagtcct atccaaatac tgaatatgcg gacggaacac caggcgacat
     3901 cttcttcttt gataacagcc aattggataa gaatgaggta aaataatatg atagagacgc
     3961 aaagattaac cgttcgccgg tttaccgccg gggactggaa agacctgtac gagtatttgt
     4021 ctgacccaaa ggtggtcatt ttcgagccgt atgaagtttt ttcggaagac gagtgtaaac
     4081 gcgaagcggc aaggcgttcc ggggacgacg ccttctgggc ggtttgcctc aaacagaacc
     4141 aaaaactgat cgggaatatc tatctcgtga agcaggattt tgacacatgg gaattgggat
     4201 atgtcttcaa ttccagctat c
//
LOCUS       DATALY010000392         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_773116,
            whole genome shotgun sequence.
ACCESSION   DATALY010000392 DATALY010000000
VERSION     DATALY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773116"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VHR42_10750"
     CDS             complement(<1..625)
                     /locus_tag="VHR42_10750"
                     /inference="COORDINATES: protein motif:HMM:NF040016.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HEX3027682.1"
                     /translation="MAFRRVEYPDGASQSEHPGLPAEFKNGGIQIQNELSLDIQAQTA
                     LILCELIRLYTCSDSSSVKDETAAAILCSIRYALDAFAKSCSTREALYLSLQKNGVRA
                     AYNAGREQIRVCFEQSKQLYEKLFKNRLTVPLDSYNDTLEHALPSFFSSYDMEFGAHE
                     TICSMDYPLAFDDMFIEGVFYIHQYLNKLELETEFCKCFSHRNIMDFL"
     gene            complement(607..1098)
                     /locus_tag="VHR42_10755"
     CDS             complement(607..1098)
                     /locus_tag="VHR42_10755"
                     /inference="COORDINATES: protein motif:HMM:NF040063.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HEX3027683.1"
                     /translation="MTPSVFESLDISLHSDRIRELMLLNQDLQGGGMMLSRTEADEIL
                     TERSRALKSRGRIELDIGVTKTLLKRLAVSPFVGRDQLVKTVSALYEVFHHIKNQTSD
                     HVGDDEVIDAIMIYFEKVCGGSAELLTGKGTERIIENYRDGQPLSDIPQNTEEESYWH
                     FDE"
     gene            1397..2332
                     /locus_tag="VHR42_10760"
     CDS             1397..2332
                     /locus_tag="VHR42_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX3027684.1"
                     /translation="MKQTTAYSIERRFFALGTVNSIRVNDPVDERVVDEAMRRVFEID
                     YKMSAFRPESEIGRLNRLAGKGPQELSTETFLLLKRSEDMARESGGAFDVTIRPLVEL
                     WGIGKKRGFIPTAGEISWAKALTGGLCLEDKTKSASFRKQGQAVDLGGIAKGYAADET
                     VRVLKTGGIENAVVNFGGNVIVLGTRPDGKPWRVGIQNPLAPTGRFLGALTMKDETVV
                     TSGSNEKFFMKDGIRYHHILDPRTGTPAQSGVLSVSAVCRRSVDADALTTALFILGPE
                     KGAGLIRKYGADVIFVMENLRVIATVGLKDRFRIL"
     gene            2419..>3420
                     /locus_tag="VHR42_10765"
     CDS             2419..>3420
                     /locus_tag="VHR42_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3027685.1"
                     /translation="MKMLHQKKSVLQIFRLLVQIAFFIFLPSLYYSTFQGIKQFYQSI
                     MQGTFDLTALLPQMIEAIAIIPLTILLGRFFCGWMCAFGALSDFIYRITGEFVKVKLS
                     ERSDRILKSLKYVWLLVLIVVFWSFDITVLNALSPWNVFGMLATFGKTPDFSYVAANL
                     FGGLLFLILILGASAVVERFFCRYLCPLGALLSLGSLLRIAKIRKPAEHCGNCRICTN
                     QCAMGIPLYQKNIVKTGECINCMKCVTACPRHNVSLAVAGEDIRPVLASTMAVAAMTG
                     MYYTVNLAERMTGVTTATAAVTPSSSAGTSSQDTSSDSSQSSSQQTVTSGDAKASSAA
                     "
BASE COUNT          858 a          768 c          899 g          895 t
ORIGIN      
        1 tgagaaagtc catgatgttc ctgtgagaaa aacatttgca gaattcggtt tcgagctcca
       61 gtttatttaa atattgatga atataaaaga ctccctctat gaacatatca tcaaaagcca
      121 gcggataatc catgctgcag atggtctcgt gggcaccgaa ttccatatca taagaggaaa
      181 aaaatgaggg aagcgcgtgc tcgagggtat cattgtagga atcaagcgga acggtcagcc
      241 gatttttgaa aagtttttca taaagttgct tcgactgctc aaaacaaacg cgaatctgct
      301 ccctgccggc gttataagcc gcccggacgc cgttcttctg cagcgaaaga taaagggcct
      361 cccgggtact gcagcttttt gcaaaagcgt ccagcgcgta gcggatggag cataggatgg
      421 cagcggcggt ttcgtctttt accgagctgc tgtcggagca ggtataaagc cggatcaatt
      481 cacaaaggat cagggcggtc tgcgcctgaa tatcaaggga taattcattc tgaatttgaa
      541 ttccgccgtt tttaaattcc gcgggtaatc cgggatgttc cgattggctc gccccgtccg
      601 ggtattctac tcgtcgaaat gccaatacga ttcctcctcg gtgttttggg gaatatcaga
      661 cagcggctgt ccgtcgcgat aattctcgat gatcctttca gtgccttttc ccgttaagag
      721 ctcggccgag ccgccgcaga ctttctcaaa ataaatcatg atcgcgtcta tgacctcgtc
      781 gtcgccgaca tggtcggaag tctgattttt aatatgatga aaaacctcat agagcgcgct
      841 gaccgttttg accagttggt cccgtccgac aaaaggggag actgcaagcc ttttgaggag
      901 agttttggtc accccgatat ccagctcgat tcggccgcgg cttttcagcg cgcggcttcg
      961 ttcggtcaaa atttcatccg cttccgtgcg agaaagcatc atgcctccgc cctgtagatc
     1021 ctgattaaga agcatcagct cgcgaattct gtcggaatgg agggatatgt ctaatgactc
     1081 aaaaacggaa ggtgtcatcc gaaacgctcc tttataaaaa aaatttataa aaagtacttg
     1141 acattaggac ttatgtgtgg tatagtatag ttgaattata atataaatta tatcaatagc
     1201 atacgagtat attatatcag aaataatata aaatgcaaga acgccgcagt aacactgcgg
     1261 cgttcttgtt gttttattat cctatctttt gccgatcatt aacagaagat tcatgggtta
     1321 atcacttttc tcacatagat agatcacatt cttccgctac aataatccta ttgaaatatc
     1381 gcggagggat ctcaaaatga aacagacgac tgcatacagc attgaaagaa gatttttcgc
     1441 gcttggaacg gtcaacagta tccgggtcaa tgatccggtt gatgaacggg tcgttgacga
     1501 ggcaatgcga agagtttttg aaattgatta taagatgtcg gcttttcggc ccgagagcga
     1561 gatcggaagg ttaaaccgac tcgcgggaaa aggcccgcag gaattatcga cggagacatt
     1621 tttgctgtta aagcggtcgg aagatatggc gcgggaaagc gggggggcct tcgatgtcac
     1681 catccgtccg ctggttgagc tttgggggat cgggaaaaaa cgggggttta ttccaaccgc
     1741 cggggaaatc agctgggcaa aagccttgac gggaggcctg tgtctggagg ataagacaaa
     1801 atccgcttct ttcagaaagc aggggcaggc ggtggatctg ggcggaattg caaaaggtta
     1861 cgccgccgac gaaacagtca gggtcttaaa gacgggcggc attgaaaacg ccgttgtcaa
     1921 ctttggcgga aacgtcattg ttttggggac aaggccggat ggaaaaccat ggagagtcgg
     1981 gattcaaaac ccgctggcgc cgaccggacg atttctcggc gcattgacta tgaaggatga
     2041 aacggtggta acttcgggaa gcaatgagaa attttttatg aaggacggta ttcgctacca
     2101 tcatattctt gacccccgaa cgggaactcc ggcacaaagc ggcgttttaa gcgtgtccgc
     2161 tgtttgccgc cgctctgtgg atgccgacgc actgaccacg gcgctgttta tacttggtcc
     2221 ggaaaagggt gcgggactga tccgaaaata cggcgccgat gtaatttttg ttatggagaa
     2281 tctgcgggtc attgccacag tgggtctgaa agaccggttt cgaatcttat agtcaatttt
     2341 atggatagca gccgatatat tctgctaaat ggtttagccg caaaagatta aatttaaatg
     2401 gaggagtatc aaacagccat gaaaatgctg catcagaaaa aatctgtatt acaaattttc
     2461 cgactgcttg tgcaaatagc attttttata tttctgccgt ctttgtatta cagcactttt
     2521 caggggatca aacagtttta tcaaagtatc atgcagggaa cctttgacct gacggcgctt
     2581 ttgcctcaga tgattgaagc gatcgcaatt attccgctga ccattcttct cggacggttt
     2641 ttctgcggat ggatgtgtgc gttcggggcg ctgagcgact ttatttatcg tatcacggga
     2701 gaattcgtga aagtgaaact gagtgagaga tccgaccgga ttctgaagtc tctgaaatat
     2761 gtgtggctgc tggttctgat tgtcgtgttc tggagttttg acattacggt attaaacgcc
     2821 ttgagcccgt ggaatgtttt cggaatgctg gcaacgttcg gcaagacgcc tgatttttct
     2881 tatgttgcgg cgaatctttt cggggggctc ctttttttga tcctgatcct tggcgcgtcg
     2941 gcagtcgtcg aacggttttt ctgccgttat ctctgccctc ttggggcgct gctctcgctc
     3001 ggctcactgc tgcggatcgc gaaaatccgc aagccggcag agcattgcgg taactgccgg
     3061 atctgcacga accagtgcgc aatgggcatt ccgctttatc agaaaaatat tgtgaaaacc
     3121 ggcgaatgca ttaactgcat gaaatgcgtg acagcctgcc cgcggcacaa tgtaagcctt
     3181 gctgtggcgg gggaggatat ccgtcccgtt cttgcttcaa caatggcggt tgccgcaatg
     3241 accggcatgt attacaccgt taaccttgcg gagcgtatga cgggcgtgac cacggctacg
     3301 gccgccgtca cgccgtcctc ctcggctggt acttcttcgc aggatacctc gtccgattcg
     3361 tcgcaaagca gctcacagca gacggtaact tcaggcgatg cgaaggcttc ctccgccgct
//
LOCUS       DATALY010000393         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_861774,
            whole genome shotgun sequence.
ACCESSION   DATALY010000393 DATALY010000000
VERSION     DATALY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861774"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..715)
                     /locus_tag="VHR42_10770"
     CDS             complement(188..715)
                     /locus_tag="VHR42_10770"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX3027686.1"
                     /translation="MNNLILCGFMGCGKTTVGRILASRLGFRHIDMDHWIEQREGTSV
                     PEIFQQRGEPYFRRLEEKAAKELSKFSGCVISTGGGAVLNPEIVRVFRSGGMIIHIDV
                     PLKVIKYRLTGNTGRPLLDGPAGDDHLLRLYESRLPAYRRAADLTIHNPNNLSAERVA
                     HKIILLLKRKRLFPC"
     gene            complement(750..1592)
                     /gene="aroE"
                     /locus_tag="VHR42_10775"
     CDS             complement(750..1592)
                     /gene="aroE"
                     /locus_tag="VHR42_10775"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX3027687.1"
                     /translation="MTAPEFRLIGHPVGHSISSQIHQRLFQLSHTNGNYQLMDVCPDE
                     LEATVEKLRNLSGFNVTIPFKQKIIPFLDEMDKRAARYGAVNTIKCENGRLTGYNTDA
                     DGFLHALTSAGIRLKGNVLICGAGGAARMMACEALERECSLTVATRTIGSAQIFADDL
                     KKSYPDADLSACALVYASGSFDLILNATPCGMYPDVEGCPIPRQVARSAKAVFDAVYN
                     PNPTMLLKKSAAAGAKTQGGLTMLVWQAAAAHEIWYDAKFRDDDILRICIEMEQILKN
                     EFAQ"
     gene            complement(1639..>2314)
                     /locus_tag="VHR42_10780"
     CDS             complement(1639..>2314)
                     /locus_tag="VHR42_10780"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX3027688.1"
                     /translation="AEAVAGFSLGECSALTASGAVNPATGFQVIRSRAQAMQKAANES
                     KSAMFAIIGAQADAIEDACKNASGYVVPVNYNSPGQIVIAGEEEAAQAAAKILSGTGV
                     RVVRLAVNAAFHSRLMENASKCFHDEIKDFSFSKPAVDFYSNITGDRTLIDDIPEYLK
                     KQMTSPVLFSNEMSSMFRDGIDTFIELGPGKTLCGFIRKGLKGVRALNIEDVKSLEKC
                     LNVLDK"
BASE COUNT          536 a          582 c          594 g          602 t
ORIGIN      
        1 aatagcccag ggtaaaccag ctaaaaaagc gatttgctgc aaaagactga cgcattcgtt
       61 aagcttccct ttctcaacag tatcgtttgc tgttacgatc tacggaaaaa cagcatttgc
      121 cattcggttt ggttcagtat agaacagaat ctcattcttg tcaagcagaa agatgaaaac
      181 tcgcgggtca gcacgggaac agacgtttcc tcttgagaag caggatgatt ttatgtgcga
      241 cccgttcggc gcttaaattg ttgggattat gtattgtgag gtcggcagcc cggcgatagg
      301 caggtaaccg cgactcatat agccggagaa gatgatcgtc gccggcgggc ccgtccagca
      361 aaggtctgcc tgtgtttccc gtcaggcggt atttaatcac tttcagaggc acatcaatat
      421 ggatgatcat tcctcccgac cggaaaaccc tgacgatttc cggatttaac accgcgcccc
      481 cgcctgtgga gataacacac ccgctgaatt ttgagagttc cttcgccgcc ttttcttcca
      541 agcgtctgaa atagggttct ccgcgctgct gaaaaatttc aggaacggag gtgccttcgc
      601 gctgctcaat ccagtgatcc atatcaatat ggcggaaccc gagacgggaa gccagaatac
      661 ggcccacagt tgttttgccg caccccataa atccacatag aatcagattg ttcatgttat
      721 cctccgcaaa aaaggcgctg gcccgttgct tattgggcaa attcattttt cagaatctgc
      781 tccatctcga tgcagatccg caggatgtcg tcatcccgga atttggcgtc ataccagatt
      841 tcatgggctg cagcagcctg ccagacaagc attgtcaggc caccctgcgt tttggcgccg
      901 gcggcggctg atttcttgag gagcatggta gggttaggat tatataccgc gtcaaaaacg
      961 gcctttgcgg agcgggcaac ctgccttgga atagggcagc cttccacgtc ggggtacatt
     1021 ccgcaggggg tcgcatttaa tatcaggtca aagctgccgc tggcatacac cagcgcgcat
     1081 gcggacaaat ctgcgtcggg ataactcttt ttaaggtcat cggcgaagat ctgcgcactt
     1141 ccaatagtac gggtggcgac ggtcaggctg cattctcttt cgagtgcttc gcaggccatc
     1201 attctcgccg cgcctccggc tccgcaaatc agaacattcc ctttaaggcg gataccggcc
     1261 gatgtcagcg cgtgaagaaa cccgtccgca tcggtgttgt aaccggttaa acgtccgttt
     1321 tcacatttga tcgtattgac cgcgccgtaa cgggcggccc gcttatccat ttcatcaaga
     1381 aaaggaatga ttttttgttt aaacggaatg gtgacattaa atccggataa atttctgagc
     1441 ttttccactg tcgcttccag ttcatcgggg caaacatcca taagctgata gttaccattt
     1501 gtgtgggaaa gttgaaaaag ccgttggtgg atttgtgatg atatactgtg gcccaccgga
     1561 tgtccgatta ggcggaattc tggtgctgtc atacaggcct ccaatgaatt acaagtttac
     1621 ggtctttgtg ttaatccgtt atttatccag gacgttcagg catttttcca gtgatttgac
     1681 atcttcaata ttgagggcgc ggacgccttt caggcctttc cggataaatc cgcaaagcgt
     1741 ttttcccggg ccgagttcaa taaaggtgtc gattccatcc cggaacatgg aagacatttc
     1801 gtttgaaaat aaaacggggc tggtcatttg cttcttaaga tattcaggaa tatcgtcaat
     1861 gagtgttctg tcgccggtaa tattcgagta gaaatctaca gccggtttag aaaatgagaa
     1921 atcttttatt tcatcatgga aacattttga ggcgttttcc atgagcctgg aatgaaaggc
     1981 cgcgttgact gcgagcctga ccaccctgac gccggtgccg ctcagtattt ttgcggctgc
     2041 ctgggcggcc tcttcttccc cggcaatcac aatttgaccc ggggagttat agttgacggg
     2101 aaccacatag ccgctcgcgt ttttacaggc gtcttcaatg gcgtctgcct gcgctccgat
     2161 tatggcgaac atggcgcttt ttgattcgtt tgccgctttc tgcatggcct gcgcgcggga
     2221 gcggatgacc tgaaatccgg tggccggatt tacggctccg gatgcggtaa gcgccgagca
     2281 ttcacccaga gagaaaccgg cgaccgcttc cgcc
//
LOCUS       DATALY010000394         6527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_774225,
            whole genome shotgun sequence.
ACCESSION   DATALY010000394 DATALY010000000
VERSION     DATALY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774225"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VHR42_10785"
     CDS             complement(<1..465)
                     /locus_tag="VHR42_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX3027689.1"
                     /translation="MKIVVNAVWRYIKQIDRVLLVLTLLAASYGLLMVYSTNLYLQKS
                     KPVFMQLIGILIGITGMVILSKIDYHVYSSIWKYLAAAGITLIVVTLILGTGTKARQD
                     NVAWLILPGGMSVQPAEYVKLIFIVTFSVHIDIVKENIDRLINVVFLGIHGVI"
     gene            825..2492
                     /locus_tag="VHR42_10790"
     CDS             825..2492
                     /locus_tag="VHR42_10790"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX3027690.1"
                     /translation="MLSDIEIAHAAKMRPIAEIAAGLGISSDELEFYGKYKAKLSDEL
                     SARLKNKKPGKLVLVTAINPTPAGEGKTTTTVGLGEAMAKIGKKAVIALREPSLGPVF
                     GIKGGAAGGGYAQVMPMEDINLHFTGDMHAITAANNLLCAIIDNHLQQGNELGIDPRR
                     ILFHRAMDMNDRALRNLVVGLGGKMNGVPREDGFSITVATEIMAILCLAKDLPDLKKR
                     LGNILVAYKSDGNPVWCRDLKVQGAMTALLKDALKPNIVQTLEGTPAIMHGGPFANIA
                     HGCNSIRATGLALRLADYCITEAGFGSDLGAEKFFDIKCRVAGLKPDAVVLVATIRAL
                     KYNGGVPKDQLKQENVAALERGGVNLRAHIENMRKYGLPVIVAINLFDTDTTAEINAV
                     EKICREEKAEFSLSEVFAKGGEGGIELAKKIVDAVEKPSDFKPVYDLCLTVEEKIETI
                     AKEIYGAQSVDFSPAAKKSIASIVSLGFGGLPVCIAKTQYSLSDNPALLGRPSGFRLS
                     VRDVQLSAGAGFIVVYTGNIMTMPGLPKRPAAEMIDIDENGEITGLF"
     gene            2589..3050
                     /gene="tsaE"
                     /locus_tag="VHR42_10795"
     CDS             2589..3050
                     /gene="tsaE"
                     /locus_tag="VHR42_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX3027691.1"
                     /translation="MQGRVASLKIITHSVAETEEAARNFAKRLAPGDVVAFFGGLGMG
                     KTSFIRGLAAGLGIRDAVSSPTFALVHEYRGPVPLFHFDMYRVGSWQDLDSTGFYDYL
                     ESEGICVIEWSENIEEALPPGTIKVGISRGPDDDTRVIDIKENGEDENTGD"
     gene            3031..3738
                     /gene="tsaB"
                     /locus_tag="VHR42_10800"
     CDS             3031..3738
                     /gene="tsaB"
                     /locus_tag="VHR42_10800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX3027692.1"
                     /translation="MKILAIESSAAPASAALWEDGLIKGEYLVNTRKVHSQTLMPMVE
                     NLLKNTGTSVGEIDLFAVSSGPGSFTGIRIGVAAVKGMSLGLGRPCAAVSTLEAIALG
                     ASGFDGVVCAVMDARRNQFYNALFDFSPAGYQRLTEDRAVSSSQLGEELLKFEKNILL
                     VGDGAELCYNNLKDLRRLLLAPEHIRYQRAACVAMLGNRAHLENTTVSAEDLLPVYLR
                     LPQAERELKKRLQEKNG"
     gene            3789..4229
                     /gene="rpiB"
                     /locus_tag="VHR42_10805"
     CDS             3789..4229
                     /gene="rpiB"
                     /locus_tag="VHR42_10805"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX3027693.1"
                     /translation="MIAVGCDHGGYTLKEEIKKHLDNLKIAYEDFGTFSEESCDYPDF
                     AEKVCRAVISGKFEKGLLFCGTGVGISIAANKIKGIRAACCSDYFSAKYTRLHNNANV
                     LCLGGRVVGPGLACELVDVFLNTPFEGGERHSRRVEKISNLENK"
     gene            4245..4877
                     /gene="upp"
                     /locus_tag="VHR42_10810"
     CDS             4245..4877
                     /gene="upp"
                     /locus_tag="VHR42_10810"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX3027694.1"
                     /translation="MKDNVFIMNHPLIQHKLTLIRDKNTGSKEFRELVGEIATLMCYE
                     ATRDLPLEEVEIETPVAVAKTQVISGKKLAFVPILRAGIGMMDGVLQLVPAAKVGFIG
                     LYRDPESLKPVDYYCKLPNDIGEREVIVLDPMLATGGSAVEAIRLIKSKGATNIKFMC
                     VIAAPEGIETLTNAHPDVALYCAAKDVKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4926..6158)
                     /locus_tag="VHR42_10815"
     CDS             complement(4926..6158)
                     /locus_tag="VHR42_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3027695.1"
                     /translation="MELKVTYDNSKYGDKWIEDLKSGFESAYSSVKVNAHADGQLSQK
                     ILPMLESGKDLPDLLFLSASDWQYMAQKGWLSDLTSLYSETVDSGKTVKQKIKPEYLG
                     YCGYEGKYWVMPWADDVPGLVYNETLFEDNGWTVPKTMNELFELIPKITSKGIAPFAW
                     SGEEISSWDTAVINWWAQYEGLGSIKNYLKMTTPEVYGQRGRLEALKTFEKIIIDTSN
                     SVPDALKISREKAVKLFYEGKAAMMPAGPWIQNLSGLSVPYGFQLKMMAVPAIDGAKQ
                     TDIKFVKPGDIAIIPEKAKNKKQAAAFLRYLSTDQALVQFTADTAVPRPFQYPVNTSV
                     GLSDFEQSVMKIWDSGKTVYEYSSNPLYRTRYFDWPSRGEPFYQIVCGSLTAQQAFEE
                     NLDYAKKHWSADSKATSK"
BASE COUNT         1626 a         1665 c         1658 g         1578 t
ORIGIN      
        1 gatgacaccg tgaattccca aaaacaccac attgatcagc cggtcgatat tctcttttac
       61 gatgtcgata tggacggaaa aagtcacgat aaaaatcagc ttgacgtatt cggcaggctg
      121 cacgctcatt ccgccgggaa gaatcagcca tgcgacatta tcctgcctgg ctttggttcc
      181 cgtgcccaga attaatgtca ccacgatcag tgtaatgccg gcggcagcta ggtatttcca
      241 tatggaagaa tagacatgat agtcaatttt agacaggatc accattccgg tgattccgat
      301 caggattccg atcagctgca taaaaacggg cttgctcttc tgcagatata aattggtgct
      361 gtaaaccatc agcagcccat aagacgcggc aagcagtgtc agaacaagca ggactcgatc
      421 gatctgtttt atgtaacgcc agacagcgtt aacgacaatt ttcatgaaaa cctccgtgga
      481 aaagcggtaa atccgccctg tttcgatgct ccgggctgaa aatgtatata atgtattata
      541 ttctgatacc ggaaagattg caagataaaa acctttgacg tatcttcgaa gaatgattat
      601 aattaaatta cggaaaacgc cggttatccg ggctttttga ctgtctgtaa cacgattgcg
      661 gcgttgccgt tcaatcggat tatgaggccg ttttccggtt cttattcggt ttttcataaa
      721 attggatcat cattgcggca ttttcttccg gccgccgccc ggatctgtcg tcaaccggtt
      781 cccacaccgg attaaatacc atattatttt acgggagaga ttttatgctc agtgacattg
      841 aaatagccca tgccgcaaaa atgaggccta tcgcggagat tgcggccggt cttggcatca
      901 gcagcgatga gctggagttt tacggtaaat acaaagcaaa gctctcggac gagctgtctg
      961 cgcgcctgaa aaacaagaaa cccggaaaac ttgtgctggt aacggcaatc aacccgaccc
     1021 cagccggcga aggcaaaaca accacaaccg tcggccttgg cgaagcaatg gccaaaatcg
     1081 gcaaaaaggc ggttattgcg cttcgtgaac cttccctcgg gcctgttttc ggcatcaagg
     1141 gcggagcggc cgggggcgga tatgctcagg tcatgccgat ggaagacatc aaccttcatt
     1201 ttaccggcga tatgcatgcg atcacggcag ccaacaacct gttgtgcgct attatcgaca
     1261 atcatcttca gcagggcaac gaactgggaa tcgacccgcg ccggattctt ttccaccgcg
     1321 ctatggatat gaacgaccgt gcacttcgca atctcgttgt cggtctcggc ggaaaaatga
     1381 acggcgttcc ccgggaagat ggtttttcca ttaccgtcgc cactgaaatc atggcgattc
     1441 tttgtctcgc aaaagactta cccgatctga aaaagcggct tggcaatatt ctggtcgcgt
     1501 ataagtcgga cggaaacccg gtctggtgcc gtgatctgaa ggttcaggga gccatgaccg
     1561 cgcttctcaa agacgcgctc aagccgaata ttgttcaaac gctggaggga actccggcaa
     1621 ttatgcatgg cggcccattc gccaacatcg cgcatggctg caattccatc cgcgcgacag
     1681 gtcttgcgct gaggcttgcc gattactgca tcaccgaggc tggtttcggc tccgatctcg
     1741 gtgcggaaaa attctttgat atcaaatgca gggtggccgg cctcaaaccc gatgcggtcg
     1801 tgctggttgc caccatccgt gcattgaaat acaacggcgg agtgccaaaa gaccaactaa
     1861 agcaggaaaa cgtcgcagct ctggagcgcg gcggcgtgaa tttgcgcgcc cacatcgaaa
     1921 acatgcgcaa atacggtctg ccggtcattg tcgccatcaa cctttttgac accgacacca
     1981 ccgccgaaat taacgcggtt gagaaaatct gccgtgagga aaaagcggaa ttctccctgt
     2041 ctgaggtatt tgctaaaggc ggcgaaggcg ggatcgagct tgccaaaaaa attgttgatg
     2101 ctgttgagaa gccttcggat ttcaagccgg tctacgacct ttgcctgacc gttgaagaaa
     2161 agatcgaaac catcgcaaaa gaaatctatg gcgcccagag cgtcgacttt tcccctgcgg
     2221 ccaaaaaatc cattgcctcc atcgtctctc tggggtttgg cggacttccg gtctgcatcg
     2281 ccaaaaccca gtactctctg tcggacaacc ccgcccttct cggaaggcct tccggcttcc
     2341 gccttagcgt cagagatgtt cagctctccg ccggagcggg atttattgtc gtttatacgg
     2401 gtaacattat gacgatgccc ggactgccga aaagacctgc ggccgagatg atcgacatag
     2461 atgaaaacgg cgaaatcacc gggctgtttt aaacggccga aaaacggaaa caacataaaa
     2521 gatgggtaac cggcgggccg ctcagcccgc cggccgctgc gcgccgtagc tcacgggcgc
     2581 ggcaataaat gcaaggacgt gtggcatctt tgaaaatcat aacccatagt gtggctgaaa
     2641 cagaagaagc cgcccgtaat tttgccaaaa gactcgctcc gggtgatgtt gtcgcctttt
     2701 tcggggggct gggtatgggc aagacttcgt ttatccgcgg gcttgccgcc ggccttggaa
     2761 tccgggatgc ggtttccagc ccgactttcg cgctggttca cgaatatcgc gggccggttc
     2821 ccctctttca tttcgatatg tatcgggttg gcagctggca ggatctcgat tccaccggtt
     2881 tttacgacta tctggagtcg gaaggcatct gcgtcattga gtggagcgaa aatatcgagg
     2941 aagcccttcc gcccggtacg ataaaggtcg ggatcagccg gggtccggac gacgacaccc
     3001 gggtgattga tataaaggag aacggtgaag atgaaaatac tggcgattga gtcatctgcc
     3061 gccccggcat ccgcggcgct ttgggaagac gggctgatta aaggggaata tttggtcaat
     3121 acgagaaaag tacacagcca gactctcatg ccgatggtgg aaaacctgtt gaaaaacacc
     3181 ggaacctccg tcggggaaat tgatctgttc gccgtatcca gcggccccgg ttcttttacc
     3241 ggaatccgca tcggagtggc ggcggttaaa ggaatgtccc ttggtctcgg ccgcccctgc
     3301 gcggctgtct caacactgga agccatcgcg ctcggcgctt ccggttttga cggtgttgtc
     3361 tgcgccgtaa tggatgcgcg gcgaaaccag ttttacaacg cgctttttga cttttcacct
     3421 gccggatacc aaagactgac ggaagacaga gccgtttctt cctcgcagtt gggtgaagag
     3481 ctgcttaaat ttgaaaaaaa tattcttctg gttggagacg gcgcggaatt gtgctataat
     3541 aatttaaaag atctccgtcg cctgctgctg gctcctgaac atatcagata tcagagggcg
     3601 gcgtgtgttg ccatgctcgg caacagggcg catctggaaa acaccactgt gtcagccgaa
     3661 gatttactgc cggtttattt gcgtctgccc caggccgagc gggagcttaa gaaacgtttg
     3721 caggagaaaa acggctaaag aaccggcctt tttcccattt gcatatgaaa cggaaggatg
     3781 ttgtgtacat gattgctgtc ggatgtgatc acggcggtta cacgctgaaa gaagaaatca
     3841 aaaaacatct cgacaatctg aaaatcgctt atgaggattt cggaaccttc agtgaagaat
     3901 cctgcgacta ccctgatttt gctgaaaagg tctgtcgcgc agttatttcg ggaaaatttg
     3961 aaaagggcct gctgttttgc ggcaccggcg tagggatttc tatcgcggca aacaagatca
     4021 aagggatccg ggccgcctgc tgttccgatt atttcagcgc gaaatacaca aggctccata
     4081 ataatgcgaa tgttctttgt cttggcggac gggtcgtggg ccccggcctt gcatgcgagc
     4141 ttgtcgatgt atttttaaat accccgtttg aaggcggaga acgtcactcc cgcagagttg
     4201 aaaaaatttc aaaccttgaa aacaaataac cggaggctgc gataatgaaa gacaatgtat
     4261 ttatcatgaa tcatccgctg attcagcata agcttaccct gatcagggat aaaaacaccg
     4321 gctccaagga attccgtgaa ctggtcggag agattgccac gctgatgtgc tacgaggcaa
     4381 ctcgtgacct gccccttgag gaagttgaaa ttgagacccc tgttgctgtt gcaaaaaccc
     4441 aggtgatttc aggaaagaaa ttggcttttg tgcccattct ccgtgccggt atcggcatga
     4501 tggacggcgt gctccagctt gttcccgcgg ccaaggtcgg atttatcggc ctctatcgcg
     4561 atccggaaag cctcaaacct gtcgactatt actgcaagct tcccaatgat atcggagaac
     4621 gcgaagtaat cgtcctcgac ccgatgctgg ctaccggcgg gtccgcggtg gaagcgattc
     4681 gtctgatcaa atcaaaaggc gcgacaaaca tcaaattcat gtgcgtgatt gcagctccgg
     4741 aaggcattga aacgctcaca aacgcgcacc cagatgtggc tctctattgc gccgcaaaag
     4801 acgttaagct taatgaacac ggctatattg tccccggtct gggcgatgcg ggcgaccgtc
     4861 ttttcggaac gaaataagtg atatgaaaaa aaacggcccg cttatcgcgg gccgtttttt
     4921 tgtttttact ttgatgtcgc cttgctgtcg gcgctccagt gttttttcgc ataatccaga
     4981 ttttcctcaa aagcttgctg cgccgtcagg ctgccgcaaa caatctgata aaaaggctcg
     5041 ccccggctgg gccaatcaaa ataccgggtt ctataaagcg gattggaaga atactcatat
     5101 actgttttcc ccgaatccca gattttcatt acgctttgct caaagtcgct cagaccgacc
     5161 gatgtgttaa cggggtattg gaaaggccgc ggcaccgcgg tatccgccgt aaactgcaca
     5221 agcgcctgat ccgttgacag gtaccgcaga aatgcggccg cctgtttttt gtttttcgct
     5281 ttttcaggaa tgatcgcaat atcgccgggt tttacgaatt ttatatccgt ctgctttgct
     5341 ccatcaatgg ccgggaccgc catcattttt aactgaaacc cataagggac cgatagcccg
     5401 gacagattct gaatccatgg cccggccggc atcatggccg ctttcccctc ataaaaaagt
     5461 ttaacggctt tctcccggct gatttttaag gcgtccggaa cgctgttgga tgtgtctatt
     5521 atgatttttt caaaagtctt aagcgcttcg agtctgccac gctgaccata aacttcagga
     5581 gttgtcattt tcagatagtt cttgatggag cccagaccct catattgcgc ccaccagttg
     5641 atgacagccg tatcccagct gctgatctcc tccccgctcc atgcaaatgg ggcgatccct
     5701 ttgctcgtga ttttcggaat cagctcgaaa agctcattca tcgttttcgg aaccgtccag
     5761 ccgttatcct caaacagcgt ctcgttgtaa acaaggcccg gaacatcgtc ggcccacggc
     5821 ataacccagt attttccctc ataaccgcaa tagcccaaat attcgggctt gatcttctgc
     5881 ttcaccgttt tgcccgagtc aaccgtttcg gaatagagag aagtcaagtc cgaaagccag
     5941 cctttttgag ccatatactg ccaatcggag gcgctgagga acaaaagatc cggaagatcc
     6001 ttgccggatt caagcatcgg cagtattttc tgactcagct gaccgtcggc atgggcattt
     6061 actttcaccg acgagtaggc ggattcaaaa ccgcttttca aatcttcaat ccacttatca
     6121 ccatatttgc tattatcata ggtcactttt agttccaatt ttgaattgcc tgcacggctg
     6181 cccgcgctat ccgtaccgga tgatgaaccc gatccggccc tgctgcatgc ggacggtacc
     6241 gccagcagaa cgcacaggag caaaaggcta attttattcc cggttttttt caaaaatgat
     6301 atcactttat tcagcctcaa tgattccgac cagatcttca attttattga cagaagcgtc
     6361 gaaagtagtt acatttccaa aaccatagga cgcaaagata aacggtatgc ctgcgaaatg
     6421 ggctgattcc caaaatcacg agcaaaggga tcgccccatt tgcatggagc ggggaggaga
     6481 tcagcagctg ggatacggct gtcatcaact ggtgggcgca atatgag
//
LOCUS       DATALY010000395         6972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U6976 k141_862485,
            whole genome shotgun sequence.
ACCESSION   DATALY010000395 DATALY010000000
VERSION     DATALY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955592
            Sequence Read Archive: SRR12844114
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:23:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,102
            CDSs (with protein)               :: 2,102
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 33 of 45
            Pseudo Genes (internal stop)      :: 5 of 45
            Pseudo Genes (multiple problems)  :: 7 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862485"
                     /isolate="SMAG_U6976"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.23 N 117.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VHR42_10820"
     CDS             <1..1250
                     /locus_tag="VHR42_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3027696.1"
                     /translation="RSILDFGSIWETLSCAGVEFVSVNEKFDTSTPGGRAMLYIIMVF
                     AQLERETIAERIKDNYYQRVKKGAWPGGPAPFGFRIADKKVNGICVIEPAPEIETVKK
                     IFAVYSISGMSLGKVAKRLTLEGVPCAKHAVWDNVSVFRILRNPAYVKADIDVYLYYR
                     NRGLIIYNDPADFAGKNGGIIVGKRLSGERKYADLSAHLFALGAHEGVIDSQEFLACQ
                     YKLDANRQIKNTGKGRLTWLSGLMHCESCGYSLKVQREPPHGRLRLVCSGRTNYYACG
                     ERHTETVEQAEAFTENAILDYIKKSRLNPDRQKKDSDRASDENALKLSLLEAEEKISN
                     LIKALSESNGVTMKYINAELLRLDEEKKRIIEGLSALSVSVPTSPFVIDFPSLSFEDK
                     KAAANMMIKKVYCSPSGFRVEWK"
     gene            1396..1701
                     /locus_tag="VHR42_10825"
     CDS             1396..1701
                     /locus_tag="VHR42_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis protein"
                     /protein_id="HEX3027697.1"
                     /translation="MKIDGNEKAMRASELFYEDRKEASRLAAEFMTEVLGSGEDHCSC
                     KIPCEIHGNCKECIISHRGARDHLPCCFWDMVNDIADHPNGLCDHEKPSIPKNKKTL"
     gene            1771..2079
                     /locus_tag="VHR42_10830"
     CDS             1771..2079
                     /locus_tag="VHR42_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis protein"
                     /protein_id="HEX3027698.1"
                     /translation="MKIDGNEKAMEGMKRFYEGNRKEAARLDAEFRAEVLASGEDHCS
                     CKIPCETHGNCKECVISHRGARNHLPCCLWNMVNEIADNPNGLCAHIKPSMRKNKKTV
                     "
     gene            complement(2188..3138)
                     /gene="msrB"
                     /locus_tag="VHR42_10835"
     CDS             complement(2188..3138)
                     /gene="msrB"
                     /locus_tag="VHR42_10835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX3027699.1"
                     /translation="MSEIYLAGGCFWGMEKYIASIRGVQSTQVGYANGKTEHPTYEEV
                     CYRNTGHAETVHVIYDPGIVSLDFLLQLYYESIDPVSVNRQGGDSGTQYRTGIYFISE
                     EDRPVIERSIALLQKRYEKPIAIEVKPLSCFSPAEEYHQKYLDKNPDGYCHIRKDKFE
                     KAAKAVVDPAAYPAQGADVLRKNLTPAQYEVTQNSATEPPFQNEFYNAFKPGIYVDIT
                     TGEPLFASNDKFESGCGWPSFSKPVDPNVIREKTDTSHGMMRTEVRSRTGNAHLGHVF
                     SDGPKNLGGLRYCINSASLRFIPKEDMESEGYGYLLGLIR"
     gene            complement(3287..3787)
                     /gene="crcB"
                     /locus_tag="VHR42_10840"
     CDS             complement(3287..3787)
                     /gene="crcB"
                     /locus_tag="VHR42_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX3027700.1"
                     /translation="MFDIGIKIQYNISGNAPLPCKNRQTANDFCFEAEIIGRFIFGGI
                     TILDCLFVGIGGFIGTVFRYLMGLIPLKSENGFPINTLLVNIIGAFAIGLIAAVSAKN
                     VSLSPRMVLFLKVGICGGFTTLSTFSLEASNLIQNGSVGVAFSYALLSVALSIAAVFG
                     AQLLIR"
     gene            4368..4640
                     /locus_tag="VHR42_10845"
     CDS             4368..4640
                     /locus_tag="VHR42_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX3027701.1"
                     /translation="MNQDKTLVCKDCGNTFVFTAEEQDFFAEKGFTNEPTRCKSCRAA
                     KRNGGRTNDREMFPAVCSACGKKTQVPFRPSGDKPVYCSDCFSKRR"
     gene            complement(5094..5333)
                     /locus_tag="VHR42_10850"
     CDS             complement(5094..5333)
                     /locus_tag="VHR42_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027702.1"
                     /translation="MEEAGEKAESMSFEYSPVRSCRRIIRKSKLGIPRFILTAGDHDI
                     ALKTPGLYAIFPIINCGGNSTAYWTFKFTWASLIL"
     gene            complement(5376..5957)
                     /locus_tag="VHR42_10855"
     CDS             complement(5376..5957)
                     /locus_tag="VHR42_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX3027703.1"
                     /translation="MKAQKRGHMHLHRAILYTFSVFVTAVFVLFLILGAMDIEARAEW
                     VGEPEPSNMKTAGKSAVVIAWPEDNLSQATPEIYFPISDLERLEIERCVMAEAGAECF
                     QGQMAVAQCILDGAIRDGISPVEALDAYGYTSARKEPSDSVRSAVSAVFDRGEMAVDG
                     EILYFYAPLFAEGEWHEENAVYVATIGGHRFFR"
     gene            complement(6114..6338)
                     /locus_tag="VHR42_10860"
     CDS             complement(6114..6338)
                     /locus_tag="VHR42_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3027704.1"
                     /translation="MEFRQTWKGREIKDYDPELQEHVNEILSHEFLPYALADHERARR
                     ILKRIITDEYGKEYLADYDGNYLGPWLEKD"
     gene            6597..>6972
                     /locus_tag="VHR42_10865"
     CDS             6597..>6972
                     /locus_tag="VHR42_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3027705.1"
                     /translation="MPDKITAIYARQSIDKKDSISVESQIEFCRRECAGLYEIYEDRG
                     FSGKNINRPAFERLMRDVEAGRVSKLVCYRLDRISRSILDFGSIWETLSCAGVEFVSV
                     NEKFDTSTPGGRAMLYIIMVFAQ"
BASE COUNT         1880 a         1706 c         1612 g         1774 t
ORIGIN      
        1 cgcgcagcat cctcgatttc gggtccatct gggagacgct ttcgtgcgct ggcgtggagt
       61 tcgtctcggt taacgaaaag ttcgacacct ccaccccggg cggccgcgcc atgctctata
      121 tcatcatggt gttcgcacag ctggagcgtg aaaccatcgc cgaacgcatc aaggacaatt
      181 attaccagcg cgtgaaaaaa ggggcgtggc ccggcggccc cgcccctttc ggctttcgta
      241 ttgctgataa aaaagtaaac ggcatctgtg tgattgagcc ggcgccggag atcgaaaccg
      301 taaaaaaaat cttcgccgtc tattccattt caggcatgtc gctcgggaag gtcgcaaagc
      361 gcttaaccct ggaaggcgtg ccttgcgcga agcacgccgt ctgggacaac gtttctgtat
      421 tccggatcct gcgtaatcct gcttatgtga aggcagatat cgacgtttat ctttattata
      481 gaaaccgggg attgatcatc tacaatgatc ccgccgactt tgccggcaaa aatggcggaa
      541 tcatcgtcgg caagcggctg tcgggagaac gaaaatacgc ggatctgtcc gcgcatctgt
      601 tcgccctggg cgcgcacgag ggcgtgatcg attctcagga gtttctcgcc tgccagtata
      661 agctcgacgc aaaccgtcag atcaagaata cgggcaaagg cagactcacc tggctttccg
      721 ggctgatgca ttgcgaaagc tgcggctaca gcttaaaggt ccagcgcgag ccgccgcacg
      781 gacgactgcg ccttgtctgc tccggccgaa ccaactatta tgcatgcggt gaacgtcaca
      841 cagaaacggt ggaacaagcc gaagctttca ctgaaaacgc gattctcgac tatataaaaa
      901 aaagccgatt aaaccccgac cggcagaaga aagacagcga tcgggcctcg gatgaaaacg
      961 cgctcaagct ttcactgctc gaagctgagg agaagatatc gaacctcatt aaagcccttt
     1021 ccgaaagcaa cggggtgacc atgaaatata ttaacgccga gctcctgcgg ctggacgaag
     1081 aaaaaaagcg catcattgaa gggctttccg ccctctcggt ctccgttccc acatcaccgt
     1141 ttgtcatcga cttcccgagc ctgtccttcg aggataaaaa agcggccgca aacatgatga
     1201 tcaaaaaagt atactgttct ccctccggtt tccgcgtcga gtggaaataa ccgtaaacag
     1261 atattatttt ctatcctcga taaaaacagg gatttgataa atataaagtc ttattgtatg
     1321 ataaagctgt atgataagga aggtattgcc aagatgcagg ttaaggctga aatccataac
     1381 taaggagcca aagcgatgaa aattgacggg aacgaaaagg cgatgagagc ctcagaactg
     1441 ttttacgaag acagaaagga agcttcaagg ctggcggcag aatttatgac agaagtcctg
     1501 ggctcgggcg aggatcactg ctcctgcaaa atcccgtgtg aaatccatgg aaactgcaaa
     1561 gagtgtatta tcagtcacag gggagcaaga gatcatttgc cgtgctgctt ctgggacatg
     1621 gtcaatgata ttgcggatca cccaaacggt ctgtgcgatc atgagaaacc cagcatcccg
     1681 aaaaataaaa aaacattata gaaaaatgcc tatgtcccag cttcggaagc acccaaatac
     1741 agattactac atagctgagg agcgtagttt atgaaaatcg acggaaatga aaaagcgatg
     1801 gaaggcatga aacggtttta cgagggtaac aggaaggaag cggcaaggct ggacgctgaa
     1861 ttcagagcag aagtcctggc atcgggtgaa gaccattgct cctgtaaaat cccttgcgaa
     1921 actcacggaa actgcaaaga atgtgtaatc agtcacaggg gagcaaggaa tcatttgccg
     1981 tgctgcctgt ggaatatggt taatgaaatc gccgacaacc caaacggtct ttgcgcgcac
     2041 attaaaccga gcatgcgaaa gaataaaaaa accgtttgat cgattaaagt catattattt
     2101 ttattgcgct cgatgataaa cgcgttatat aaataaagtg caggacgctg tgtcccgcac
     2161 tttatttata ttaaatttac cagccgctta ccttatcagc cccagcagat atccgtatcc
     2221 ttcgctttcc atatcttctt tcgggatgaa ccgcagggac gcgctgttga tgcagtaacg
     2281 cagcccgccc agatttttgg ggccgtcgct aaacacatgg cctaaatgag cgtttcccgt
     2341 gcggcttctc acctcggttc tcatcattcc atgagaagta tccgtttttt cacggatcac
     2401 attgggatca accggttttg aaaagctggg ccagccgcag ccggattcga acttatcgtt
     2461 cgaggcgaaa agcggttctc cggtagtgat atctacataa ataccgggtt taaacgcatt
     2521 atagaattcg ttttgaaacg gaggctcggt ggcgctgttc tgagtaacct catactgcgc
     2581 gggtgtcagg tttttgcgca gaacgtcggc gccctgtgcc ggataagcgg caggatccac
     2641 cacggctttc gccgctttct caaatttatc ctttcggata tggcaataac cgtccggatt
     2701 tttatccagg tacttttgat gatattcttc tgcgggactg aagcagctaa gcggttttac
     2761 ctctatggca atgggttttt catacctctt ttgcagcagc gcaatggaac gctcaatcac
     2821 cggccggtct tcttcgctga taaagtagat tcccgtccgg tactgcgttc cggaatcgcc
     2881 gccctgccgg ttaaccgaaa cagggtcgat cgattcataa tataattgca gcaaaaaatc
     2941 cagcgaaaca atcccaggat catagatcac atgtaccgtt tcggcatgtc ccgtgttccg
     3001 gtagcagacc tcttcatagg taggatgctc ggtcttcccg ttcgcgtagc ctacctgcgt
     3061 cgattgaacc ccccggatcg acgcaatgta tttttccatt ccccaaaagc agccgcccgc
     3121 aagatagatt tccgacattc cgcacctccg ttcttctgtt ttgccttgtt gtcaccctat
     3181 gatcttgcct ctgtatgccg ttctctattc aaacgattcc gcatatcccg cgcgccctcg
     3241 gttacgcgcc acagaccgga tgtttccccg ccaacacagg tgctatttaa cgaataagca
     3301 actgagctcc aaacaccgca gctatcgaaa gagcgacact gagaagtgca tacgaaaacg
     3361 caacgcctac ggatccgttt tgaatcagat tcgatgcctc caaagagaag gttgaaagcg
     3421 tagtaaaacc tccgcatata ccgactttaa ggaatagcac cattcgcgga cttaatgaaa
     3481 cgtttttggc cgatactgcc gctatcaaac caatggcaaa cgccccaata atattaacca
     3541 aaagcgtgtt tatcggaaac ccattttcag actttaatgg gataagcccc atcaggtaac
     3601 ggaagacggt accgataaat cccccaatgc cgacgaacag acaatctagt atagtaatcc
     3661 ctccaaaaat aaaacggccg ataatttctg cctcaaagca gaagtcatta gccgtttggc
     3721 ggttcttgca tggaagcggt gcatttccag agatattata ttgaatcttg atccctatgt
     3781 caaacatatt cgtagcggac gcgcctaaga cgaatgaaca gattatgcga atgaaagttt
     3841 tgagtttttt attacaaccg aatatgacat aaatgataac ataatattat agattttatc
     3901 tttttatggt agattattcc atgtaagaga attgagtttt cccgtattgc atatcatttc
     3961 atgctttaca taagggggaa gacgcttgat tcattgtgat aaagaccaag caaatgaaat
     4021 caatctttta ctccggacgg caatgcaatc cattccacag ttgggtatat atagtatttt
     4081 tacatgcccg aactgcggcg gtaccgccgc agcgatcaaa tacggagaga atgaaataca
     4141 ggtttggtgt ctccgctgca gtatcgttgt tgccgacagc agggatgccc tgcagcacat
     4201 ctgttcttta tactgaattg ctggggaaaa agtcgataaa tgacgaatga tatttaaaaa
     4261 tactgaaatt gttttattga caatgaaaat aaaatagttt atgctttaga tagtgctctt
     4321 agcttctaat ggcaagagcg caattttttt aaacggaggt tcaatacatg aaccaggata
     4381 agaccctcgt atgtaaggat tgcggcaata cgtttgtttt taccgccgaa gagcaggatt
     4441 ttttcgctga gaagggattt actaacgagc ccacccgctg caaaagctgc cgcgctgcga
     4501 agagaaacgg cggaagaacg aatgacagag aaatgtttcc ggccgtctgc tccgcctgcg
     4561 gcaagaaaac ccaggttccg ttcaggccca gtggggacaa gccggtctac tgcagcgatt
     4621 gtttctcaaa acgcagataa gttagaaaga taatcagccg aaaagaataa tcgtttgact
     4681 aaaaatacat ctttaaggaa gatgtatttt ttgtattcca ttgctttttt gagcctggtt
     4741 tcctattttt gagttatttc cagctatttc ctatgaccct ataattgaca aaattgcagt
     4801 tatataatat attttatgtg aaacatttga ggaacagcaa gttgagttat tcccggtaac
     4861 gttaaagcaa tggtttcttc ttttttacat aaatttgaga aattatttca ctttcttata
     4921 atgtattgag aaatttgaga aaatacgcgc ttaccttgcg atcaattcat atcgaatcaa
     4981 catatattcc cttctattgc taagctattt taagggaggt atagcgtttg acccgatgtg
     5041 acgaaaaaca aataggcaga atcaatctat ttcttaaagc ggcgcgtcag tctttacagg
     5101 atcagggatg cccaagtgaa tttaaatgtc caatatgcgg tgctgttgcc accgcaatta
     5161 ataatgggga aaatggcata cagacctggt gtcttcaatg caatatcgtg atcgccggca
     5221 gtaagaatga agcgcgggat tcccagttta cttttccgga tgattcgcct gcatgatcta
     5281 accgggctgt attcgaatga catactttct gctttttccc ccgcttcctc catttaaaaa
     5341 accgccgata gggcggcttt tttattttgt ccttttcagc gaaaaaagcg gtgtccgccg
     5401 atggtcgcca catacaccgc gttttcttcg tgccactcgc cctcagcaaa cagaggcgcg
     5461 taaaaataca ggatttcacc gtccacggcc atctccccgc ggtcaaaaac ggcggacacc
     5521 gcgctgcgta cactgtccga aggctctttt cgcgcgctcg tatacccgta agcgtccagc
     5581 gcctccacgg ggctgatacc atcccggatt gctccatcca ggatacattg cgctacggcc
     5641 atctgtccct gaaagcactc ggctccggct tcagccatga cacaccgctc gatctccagc
     5701 cgttccaaat cggatatcgg aaaatagatc tccggtgtgg cctgggacag attgtcctcc
     5761 ggccacgcga taacaacggc ggatttgccc gccgttttca tattcgaagg ctccggttcg
     5821 ccgacccact cagcccttgc ctctatgtcc attgctccga ggattaaaaa caaaacgaaa
     5881 actgcggtaa cgaaaacgct gaacgtataa agaattgccc ggtgcagatg catgtgtccc
     5941 cttttttgag ctttcatggt ttttcgccgc cttcctgaat tatttgcacc ttcccgtcgc
     6001 ttattcaact gcggagcata tatatgtatt gccgcaccca ggctgtggca gaatcttgca
     6061 tattgcatgc ccgctttggg cagcttgtcc ggtttaaaaa cagcgaaatt gatttaatct
     6121 ttctcaagcc aggggcccag ataattgccg tcataatcag caaggtattc tttgccgtat
     6181 tcgtccgtaa tgatccgttt taatattctt cgcgcacgtt catgatctgc cagcgcgtaa
     6241 ggcagaaact catgggacag gatctcattg acatgttcct gcagctcagg gtcgtagtcc
     6301 ttaatctccc tgcccttcca ggtttgtctg aattccatcg ccttaagccc ccttctgacg
     6361 aacctacctc cgacggagcc ggtattaaaa atctatggcg gacagccccc ccatatgaat
     6421 gattttttaa acgtcttaaa ccggatgtat aatctcagat tttaagccca gcctgccttc
     6481 ccaataaaaa aacctgccca aacagggcag gacgacataa ttttgacgaa atgtttatat
     6541 gcctttttat ataattaaac tgtgaatttt tcaaatatga agaggggcca cgagctatgc
     6601 ccgacaaaat cacggcgatc tacgcccggc agagcattga taaaaaggac agcatctcgg
     6661 tggaaagcca aatcgaattc tgtcggcgtg agtgtgcggg tctttacgag atctatgaag
     6721 accggggctt ttccgggaag aatatcaacc gtccggcctt cgaacgcctt atgcgcgacg
     6781 tggaagcagg ccgggtatca aagcttgtct gctatcggct cgaccgcatt tcgcgcagca
     6841 tcctcgattt cgggtccatc tgggagacgc tttcgtgcgc tggcgtggag ttcgtctcgg
     6901 ttaacgaaaa gttcgacacc tccaccccgg gcggccgcgc catgctctat atcatcatgg
     6961 tgttcgcaca gc
//
